LOCUS       DAWDHD010000001        58997 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_54_length_58997_cov_14.177717, whole genome shotgun sequence.
ACCESSION   DAWDHD010000001 DAWDHD010000000
VERSION     DAWDHD010000001.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 58997)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 58997)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..58997
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54_length_58997_cov_14.177717"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT        15253 a        14895 c        11430 g        17419 t
ORIGIN      
        1 tgacgcaata cctgatgcat ctgagactga cccatgcctg ccatctgtta gagagtacct
       61 ctctgccggt tacagaggtt gccctgcagt ccggcttttc taatgtaagt cattttatcc
      121 gtagctttca cgcttctttt cggatgtcgc cgctgcaata ccgcaagaaa cgggatcagt
      181 ccttcacata aattctgata ttggccggtg ttaccggata ggttccggtg tcaatggttt
      241 tgggtatact ctgtccttca aatactgcct ctattttttc cagtgtcatg gatccccagc
      301 tctccggacg ctgcgcaatt gcagcatcta catagccggc cttcatgaat tctccaactt
      361 catccgtgaa gtccactgtt accagtttta ccttcttccc tttggactta agatatctgg
      421 caaaagccaa tccccatcct acatttgtag cgcacagtac agagatatcc ggatgctccg
      481 taagaagccg gtcagttaat tttgttgctt cttcagcagt aggttctccc ggaccggtca
      541 tatcataata aggaattctg ttttctttca gaacatctac aacaccgtgg tatctgtcct
      601 ctacagtctc aatgaggtta tccttccact tgataatgcc aacggcaccg tcggctccca
      661 aggcttctct cacagcctct ccggtactcc gtccgcactg ataactgtca gttccgatga
      721 gtgtgcgata gggaacacta tcaaatgcag acaggatgaa aatcacctcc acacccttct
      781 ctgccatctt ctgcagcaat ttttccactc tgagatccgc aatgggtgag atacagattc
      841 catcataacg ctcctccaac acctttttca gaaaatgcac catttccaga gttcctgctt
      901 ctcctcttcc cttaggtgca aataccgtaa tatcaaaatt cagaattttg gcagtgcgat
      961 aggcctgttt cgtcgccgga taccagaaag gatcatccag atcccatacc atggcaattt
     1021 tcctgcgctg taccggtgca taatccaccg gtatattagc caggatttca ttcagctgat
     1081 ttaattcgct gttcaatcgt tcggatacat gccccataag gctggcattc tgcttctgtt
     1141 ctgcagtaat tcccgccacg ttttccgagg atgccgatgc ctgactggag attgttgcaa
     1201 tagattccat gacttccagt agctcttcct tcttcccatt gacttcatcc atacgttctg
     1261 tgaaaaaaaa ctgcgcttct gccagttctt tcacactgtg atttatattt tcaaaggaag
     1321 aaattgtatc ccctaccgcg gttttctgtg cttcaaaagc tgcatcggat tcctccatga
     1381 tctctttcag ttttgttaac gaacctataa tctcctgaat ggatgcattg atactctgac
     1441 ttgccttatg acattcctcc gacaattttc gtacttcata tgccacatga gcaaatcctc
     1501 ttccggcttc cccggctctg gcagcctcga tggatgcatt taaagacagc agattcgtct
     1561 gcttggcaat actatagaga acacttgtga cagattcaat ctttgcattt ttatccagaa
     1621 gcgtacgaat ctcttctgtg atcgtatcca tggaaccttt gagtttttcc tgtgttttcc
     1681 ccagatgttc cactctttgt ctgccaagat cactctgttg ctgcatctgc tgaacctgtg
     1741 tctgcatagt tgcagtctcc tgatccatat ccgtgatctt cgctgtgaat tcctcggcta
     1801 ttttccggca ttgcaataca tctctttcct gccttgaggc accttccgca agcacctgta
     1861 cgccatgtcc caccttatcg gaggactctg tcagcccctc tgccgcacct tcaaattgtt
     1921 cggataccaa tcccagatta ttgacagtct gtttcaagga ttgtagctgt tccttatatg
     1981 attcttctgt ttgtccgctg cttaccgctc ttttgttcca taaaggcata gattatccct
     2041 ccaatgctct tctcgccagt tccaggcatc ccatgatgcc ctgatcatca tgcagtgacg
     2101 caggtactac ataatgctcc ggatcctcca gttcttctgt tatcacatag ccattgatat
     2161 aggtaatcag ctttttgcgt attaatggga atagttgctc ctgatgcatg acaccgcctc
     2221 cgagaatgat catttccgga gaaagtacca tcacataatt tgtcaatgcc tgcgcaaggt
     2281 agtctgcctc cagatcccat acttccggac aatctgccag ttccactgct tttttgcccc
     2341 atctggcttc tatggcagga ccagccgcca ttccttccag acaatgatcc ttgtgatagg
     2401 ggcattttcc ttcataatgg tctgtatccc gttttgtgat gaggatatgt cctgcctcag
     2461 gatgaagcat accgtgcagc agcttcccgt caatatacac gcctgctccg attccggttc
     2521 ccacggtcaa atacatgacg caatgtttcc cttttgcctg cccgaaagta acctcaccca
     2581 gtactgaacc atttacatcg gtatcaaatc ccataggaca ttttaacgct gctttcatgc
     2641 ttcccaaaaa atcaaagttt gtccatgcaa gtttagatgt ggtagtaata tatccatatt
     2701 tttccgatgc tggatccagt tccaccggtc caaaacttgc aattcccaat gcgtctatct
     2761 gcttgtcctg aaaatatgcg atcaatttcg gaatggtctc ttccggcgtc gttgtagcta
     2821 tagatacctg ctcatagatc tttcctgttt catctccgat ggcacagacc atttttgttc
     2881 cgcccgcttc taatgctccc aatttcatag tgattccttc tttcttttta ttttttatct
     2941 tccgatctat tcccataggt tttctgatat aaatgcagcc agggtgtatg ctctggcaat
     3001 ctggagaaat ccttttgatc atagatttcc tgaatctgtc cattccgtat ccggatcaga
     3061 cggtcagcca gagccagaga atcggcaaga ttcaccgcca ggatcaccac tgcaatattt
     3121 tttttcaaaa accgttccat cagctcccag atatgactgc gcacggacac ttccgcattt
     3181 ttaaacggct gtacgcacaa cacgacctgc ggatgctgca tcagaattct cgtatatacc
     3241 agatcatact tctgctgcct ggtcaggttc cacaccggag tgtcaaatac gtcctccccc
     3301 aacagatctt tttgttcctc ctggatactt ctccggattc cagactgatt ccacacaccc
     3361 cggattcgat gatccatggt aaaacatagg ttatcaaaat aactcatatg aggaaacagc
     3421 atagtctctg tgggtagctc ctggattacc gcaatatctc tgtttttctc acttttttgt
     3481 gtccctttcg tcagataaaa gcttcctttc tctggaatct ctccttgaaa cagctccaca
     3541 aaatcagcaa tgcaacgatt atccagatcc tgcaatacaa cgcattctcc ctgcccgacc
     3601 cgaatcgtaa tatctttcag attaccatat tgtatcttat ccatgatcaa tacggtctgt
     3661 ctgtctgtat ctgcttcctg tcgtctatgt tgagatctca cccacctgtc atattcctcc
     3721 acactctgta gcggaaatgt atcaggaatc ggatcacctc tctgaaacgt tttgatgatc
     3781 tgcccgttta ccattaatgc catacgatca cacagatagg ttgcttcttc aaaatgagcc
     3841 gtgatgtaaa tgaaagaaat tcctctttct gcgtacagtc gtatgatcct gtgcaattcc
     3901 tccagttcgc tctcgctaat aaaagtactg acattttcca atacgatcag ctgacttcct
     3961 gccaccaccg ctttcaaaac ttctgtcaca aacttttgaa atattgtcag ttcgctgaca
     4021 taagcccccg ccgggatatt gatcccgatt tcatccagaa aaggctgcag cagtcgttcc
     4081 aacattcccg gatgaatcag atacttatga aatcctctcc gcaggacaaa aatattgtct
     4141 accacggtta gatcctccgc cagactgctt ttgttgcgga tgatactgat cctgttcgtc
     4201 cggttatgcg acctctgcca ctgattgacc aattgctcac gataatatac atatccgtag
     4261 tgaagcggca tattttgttg caacagtttg agaaaagcat ccattccata acgattgacc
     4321 gggatcagac cgaatacctc tccctttcgt accgttatat taaaattatc caggagaatt
     4381 cttccctgtt ccttgtaggt caccttctcc atgcgcagca cttccctgat caacagtagt
     4441 tttcctgcct ttcttccata tgaatcagat tacgatcact gtagattaca ggaattctta
     4501 tcttcacatc cgtgccaatc tccggcatag aatacacatg aattccatat tcttctccaa
     4561 atagtaaatg gatacggtta ttgacattta ccagtgcgat cccgcctttt gtctcggatt
     4621 ctcccgttgc gagaatacct ccgctttttt gcaatctttc attgatatcc gctactttgc
     4681 gttcttccat gccgttgccg tcatccgaga tacacagaag cagacaactt tccgtcattt
     4741 ccagtaggat ccggatatgt cccgttccca gtttgcactc tgtcccatga atgatactat
     4801 tttccaggat cggctgcaag gtcagtttgg ggatacgaca gctgtaaatc gctgcttttt
     4861 cctcgggatc acattcaacg gaaagctgca gtctgtcgcc gaaacgatac ggctgaattt
     4921 taaaataagt catacagttc tgcaattctt cttccactga caccagattt tctactttgc
     4981 tgatggtata gcggaaaaaa gtggccaatg cctctgtcat atctgcgaca cttaccagtc
     5041 ctgcaatcag tgcctcacta cggatgctct ccaatgtatt atataaaaag tggggattga
     5101 tctgattttg taatgccaga tactgtgcct gtctcttatt caggttcagc atctcctgtg
     5161 actgcatcag atactgcatt ttgctgacca gactctccag ctccggagaa atgtggatcc
     5221 catcttctcc caccagattg ctggtggtat aaccgtccag aaataattct atccgcttct
     5281 cgatacgccg gtaaggcagc catatctcca ctacagcata gcagatacca agcaccaaca
     5341 ggcaccctcc aaagatcacc gggaaccagc ctgccccatt ccccctgatt ttttcataga
     5401 taagatatcc caagagcgca gccagaaaca acaccataaa actccacgca cacaatgtcc
     5461 gaaagttcag ataatgcatt tttccctgct tcatacgctc tcctctcaac cgtacagctt
     5521 ccggtactct gccggattta cgccggcaat cttatgaaaa gtctgggtaa aatgctttct
     5581 gtcattatat cctacccgct cacagatctc ggtcacagac aatcctgttt ctctcaacag
     5641 cgttttagcc tcatccattc ttacccgcat cagatatttg gcaaaccctt ctcctgtttc
     5701 cttcttaaat agggcactaa aatatgctac actgaaaccc acatggtcac atacttcttc
     5761 caatgtaata ttttccgcat aatgtttgtg aacatactgc tttgcctggc ggatcggccg
     5821 ggaagtctcc ccctgtctgg cttccagagc ttctgtcatc agttcttcct gcagcgttga
     5881 cagttcatga aacaattctt ccgcagagcc acaatgcata cacttttctt caaatatagt
     5941 gggagatgta gctcttacac gcacaataaa aaatcttcct gctgacagca ccgtttcctg
     6001 aatttcccgt cctctgacat ccggtacact catcaccgca tttttcagtt cttctccgca
     6061 cttgctggct tctgccggat ccagaacttc tatggcatgt tccatttctt tacaatatcg
     6121 atccagaata tcctgcattg gtaatccgga tgccggaggt acttcttcca gaagtcttcc
     6181 ccagccttcc acgatcctct cccgaatcgc aagctctgct tttcttatgg atgcggtcaa
     6241 ttgttccgca tccactgtca caccgccaat ggcaagtgaa aatcttaccg gtcccaacat
     6301 ggaacggttc gcttccagtt gtcgcaaaaa ttccaaaagc aaagttctta ttttatccgt
     6361 atcttctttt ataaaattaa gaataccata cccggaatct ttcacataat caaaaaggca
     6421 gtcattacag ttttttgaca ggcaatgata gaacaactct ttcgtttttt cacagatcac
     6481 agtctgtgct gccttactca tttgttccgg atcatatcct gctttcacca ctatggcctg
     6541 ataacaatca gcttccgcct taaaataata gttctcctgc agctgcactg cggttggatt
     6601 ataggcatct tctacaagat ccttcagcaa cgcatatctg aggcgattta catcgctctc
     6661 actgactctc cgctcagcca tttccatacc ctgggcagat ttctttgcat ccagccgctc
     6721 accaattttt cgcagtgttt cgttcaattc gtcctgttta ataggtttta aaaggtaatt
     6781 tcccacacca agggaaagcg catttcgtgc atactcgaaa tgtgcatatc cactgataat
     6841 gatgatttcc agttccggac atatcttttt tgcatttttt atcagctcca gcccgtccat
     6901 tcccggcatg cgaatatccg tgatcagaat gtccggtcta tattgctgca gcatatccag
     6961 tgcctgtatg gcattttctg cctgtgccgc catctgcatt cccagtttgt cccactcgac
     7021 caatgcctgg acaagcttgc agatcagctt ctcgtcatct acgatcatta ccttccacat
     7081 aattttagcc tcattattag cctttttttc cataaatata ggtttatttt agcactcgtt
     7141 taaatacaat tcaacaggaa tatcccatat ttccggatat tcctgttgaa tttttatatt
     7201 tatggaagag aaaagatttt gcttactgct gtttggtcag aacagatact cctgtatcgg
     7261 ttacagcacc accgagactt tcaccattga tggcttttac agctgctttt acaccttcat
     7321 accccataac atcgggattc tgagccatgg tacacaacag atatccgtca tcgatcagac
     7381 ccaggatagc atcagactta tcaaagccta caccgattac gttatcctta ccggaagcct
     7441 tgatggcgtt accggcgccg gtggtggaac cttcgttaca tccgaagata cctacaacgc
     7501 cctgggtaat atagttctct gcaatacttt gggattttgc tgcatcgcct tcgccgtact
     7561 gagtctccag aagttcgaaa tcagttcctt caaatgcaga acggaaacct tcttctctca
     7621 taacgcagga atcggttgct gcatttacat tgatgatacc aatggaacca ctggtgacac
     7681 ctgcatctgc caatgctttc ttcatttctt cacctgctgt cttacctgca gcattgttgt
     7741 cggtagagaa agtagcttct gcggatacgt tcgcagggga atctacgtag atcagcttaa
     7801 cgcctgcatc tgatgcctct ctcagtgcgg aagaaatcgc atcgggaccg tttgctgcta
     7861 cgatgatagc ctgtgcacct gcactgactg cgttgtttac acactcgatc tgctgagcat
     7921 catccttggt attgggagcc ataaactgaa ctgttacccc cagctctgct gctgccttct
     7981 gtgcgccctc attcagagtt acccagtgct gatccagaga atccatggtg atcagagcga
     8041 tcagagtatc ggatcctgcg gtggttcctg cgtctgttgc agttgaagcc tgctctgtgg
     8101 tggtggatgc tgccgcactg ttctgtgcct caccggtggc tgcggttcct gcgttattgc
     8161 tgctgccaca tccggtcagc attgctgttg tcatgcataa tgcacaagct actgataata
     8221 cctttttcat tttgtaaacc ctccttaaaa gtgttcattg atttatctta gcatcttaat
     8281 tgatgtctaa ttcttagccg gtttcttctt aaatacacca ttccttacaa caacatccag
     8341 aagtactgca aacactacga tgataccgat aaccagtctc tggataccta cctgggcgcc
     8401 aatcatattc aggccattct ccagtgtctg ccatacggaa gcacctgcca gagttcccaa
     8461 cagcaatccg gttcctccca aaggagaaat accaccgata acgcctgcgg ctaccgcata
     8521 catctcgtac atgttaccgg cttccatgtt acccattccg gcctgaccgc agaggatcaa
     8581 tcctaccaca aaagaacaga atgcactgac cagataggtc ttggtggtgg tctttactac
     8641 gctgacgccg gacagctttg ctgcatccac attggaaccg atggcataaa tatgtctgcc
     8701 ggtacgtgtt ttggacagca gaaagaagaa tacgataaag ataataattg caatccagaa
     8761 agtattgtaa acgcccaatg ttttaccata gtagaaaaca ttctggaaag tctctccggc
     8821 atctttgccg acaccggatg cgatggcatc cgtattacgg ttaccgttta ccatgtaagc
     8881 aacacctctt gctacaaaca tggttcccag agtagcaatg aagggaggca gtttccactt
     8941 acctaccaga ataccattca gtaccccgat gatcatgcaa catacaaaag taatcaggat
     9001 agctactaca gggttcacac ccatggtcat cagtgttgct gcgatcatgg cactcatacc
     9061 taccacagat ccaatggaca gatcgatatt accggtgatc agcacataac tctgaccaat
     9121 accaatgatc agatactttg acatggatcg taacaggttc attacattct gaccggaaaa
     9181 tacggccgga tttataatac caaaagcgat atagatgatg atcagaccgg caaaagcggt
     9241 aagggctcct cccattcctc tgatcgcaaa aattcttttc aacggacttt gctttttcat
     9301 aatgatattt tcacctttct tatggttata atttaagcgt tagctgcgat cttcttctcg
     9361 aactgggttg ccagggtcag gatctcattc tgcgttgtgt ttctggcatc caattcccca
     9421 gtaatccttc catcacacat tacaatgata cgatcggcta ttcccatgac ttccggcatc
     9481 tccgaagata cgaacattac ggcaattccc tgttttttca gttcgttcat gaggttatag
     9541 atttcaacct ttgctgccac gtcgattcct ctggtgggtt catcgaagat aacgactttt
     9601 gaattccgtg ccagccattt tcctacgact accttttgct gattaccacc agataagtta
     9661 ccggcattga tgtccacgtt cggtgttttg atcttcaggt tttctactgc ctcgtcacac
     9721 atctgatctt ccttcttgct gctgactact cccagtttgt tgcagagaat atccagatta
     9781 ggtaatgcaa tattgtgccg gatgctcagt ttggtacaca atccatcctt tttccggtct
     9841 tccggtgcca atacgattcc tgcgcggatt gcatcattgg gatttgtgat ttttaatgtt
     9901 ttcccttcca gaatgatctc tccgctgtcc tttggatcca ctccgaagat tgctcttgtc
     9961 gtttctgttc ttccggcacc catcagtccg gcaaagccca cgatctcgcc ttcatacaat
    10021 gcgaaattga tatcccgcac catgcgacct gcgtttaatc ccttgacttc cagaatcttt
    10081 tccccttttt tacacgactc tctcgggaac tgttccttga tctctcgtcc taccatattg
    10141 gctataatct ggtccatcgt ggtatctttg aaattcatgg aagtaatata ctgtccgtct
    10201 ctcatgatgg tcacacggtc tacaatatgc tgaagctctt ccagtctgtg ggatatgtat
    10261 acaataccgc atcccttgga tcgcagatcc ttaataatgc ggaacagatc atctatttcc
    10321 tttgctgtca gtgcggatgt gggttcatcc atgatcagga ttctcgcatt gaccgacaat
    10381 gctttggcaa tttcgaccat ctgctgtttg gataccggca gttctcccac gacctgcttc
    10441 gggtcaatat cgatcttcag atcatttaat atccgggagg cttccgcttc catatcaccg
    10501 tttgccaata caataccttt tgtcttctcc ctgcctaaga acatgttctc tgctacggac
    10561 aggtgcttac acatattcag ttcctgatgg ataatagcaa ctccggcttc ctgtgcttgt
    10621 ttcggtgtca gatccccata ctccttgcca aagatttcca ggcttccact gtctctggta
    10681 tacacaccgc tcagaatctt catcagcgta ctcttgccgg cgccattctc tcccaacagg
    10741 gccatgactt ctccggatcg caattcaaac tgtacatgat ccagtgcttt tactcccggg
    10801 aagcttttgc atatatcctt catggatact atgacttctt tttcactcat accttctctt
    10861 cgtccttccc ttccattaat aaataggcta attttagctg tttttgcgtt ccccttaact
    10921 ttatgttaac agtatagcaa aagccccctt caaagataag gggactatct ttcttattga
    10981 agggggtttt ttatgcacta tgaatatgaa attctaaatt acacctttgt acctatacaa
    11041 gtttacgatt ctgtttttgt ttctcttctt tttatatcgg cagtttgtat accagttttt
    11101 ctttccagct cttccatcat gacattgctg ttacgaaaaa cacataaaat cactcccacc
    11161 atcagggaat tggccagaca gttcccaagc ccataagcca gaaatggaat ggatatggtg
    11221 tagtaaatgc caaatccaaa attcaccaga accgcattga cgatccgaag caggatgctc
    11281 agactgcaca cactgcccaa cagcaatgcc gcacgattac tctgacgcag agcgacacgg
    11341 agtgcataaa gcgcaaaagc aaacagtgcc acgatcacca atactcccac aatgatacca
    11401 aaccacagga aaatgctgtg aataatgtag gttgtacagc tctcctgtga cagggaccat
    11461 gtcccgttat tttgatctga ggtaaaaagt aatgcctcac tgatctttcc cccacccaac
    11521 aatgaaaagc catgctgttc ctgctgaata ttgctggcaa tataaccggt ctcccctttt
    11581 cccatccggt aatccatccg ccgccaaata gagtaatctc ttttttcccg gcagaatgcc
    11641 ccgtttgatg gcaaggaaca aaattacgat agataacaag aaagcttcca tcataccggc
    11701 agagcaggta atatcctgcg ataacagcag catcgctgcc agatacaccg catccagaat
    11761 caggattacc ttccagctta aggttcgcat gcgatataaa agcaaggcat acagaaccgg
    11821 aagaaacatc cacagcagat agacagcttc gtatctgtca tgataactgc cactccacat
    11881 atgtgtcccc agcatgctgc ctgccagccc tgcagctata aaaagcgccg tcagcccata
    11941 gctgaaacgt accagtttca tatagttttc atagagaagt ccgagaatca gcaacagtcc
    12001 gattccgtta tacaggatca tattacgcag ataattttcc gctcccacca tctccatatg
    12061 ggaaaacaag attccctgca tgacaattcc caccagtgtc agtatcaccg tgatgccaaa
    12121 caacactgtc ggaaacttcg gcctgtggat ccggttcaat tccctgccgg tctgcagcgg
    12181 atcccccatc tggcgtaccg cttcctgtgc ggctgccagg ggtgtcattc cctcccggtg
    12241 atagtcctcc gtctgttcct caatgtgatt gccaatctcc ctgcggattg cgcatctggc
    12301 atggggattc tcgatctgtt ctcctatggc atccagataa tcccggatgc ttccggcatt
    12361 ccgccaatcc gtattctctt ccgccgccgg ttgttctgcc tcctcatgcg ctgccagcac
    12421 tacccttttt tcttcctttc ccataatcag caccctctct gaagactcaa cactccggaa
    12481 actgcagtct ggtattcctg ccattccttc ttcttttctt ccgcatgctt ttttcctgca
    12541 ggagtcagct ggtagtattt tctcgtcttg ccggaagtat tgtcctcgta agacaccaca
    12601 tctccacggc tctccagcga gtgcagcaga ggatacagtg tcccggcctt tagttcgaag
    12661 acattgttag atctctctcg cagtgcctca atcatctcat agccatacat gtccttggtc
    12721 tccaatagac gcaggatcat cattgccata cttccctgta ccagattcct atcgattgcc
    12781 atatgtatac gctcctctct atatgggctt tctacacaca atatatatcg tcagtctata
    12841 tatgccaagg aaattattct cacttctctt ttgtgctttc tttcgcacgg tatcgtatgc
    12901 tatactgttc ccagatattt tcttgatcat ttctacaaaa cccaacatta tataaaataa
    12961 aggaggtata caatgaaaaa aatatggatc gtcggttccc tcggtcacat ggggcaggcg
    13021 ttgatcaggt tgttagacat gatggaatat gaattatacg aaaccgataa ggacgagctg
    13081 gatatcacgg atgaacatgc agtttccctg ttcatgcacc ggaatcgtcc cgatgtcgtc
    13141 atcaactgtg caggatatca tccttctgcc ggtgcagcgc agtcggagac ggatatggct
    13201 tacaaggtaa acgccgtagg tgcgaggaat ctggcacagg ctgcggagag tattcaggca
    13261 aaactcatcc agatctctac ggatgatgta ttttccagcg catccgacac accctataat
    13321 gagttcgatg atgtaaatcc tgtgggcctg tttgcgaaat ccaaatatgc cggagagaaa
    13381 ttcgtcatcc agctgatgac ccgctatgtc attatccgca gttcctggat ctacggcatc
    13441 ggcaaagact ttttgaatga agtactggaa gctgccgcag attccaaagt atccaccatg
    13501 gaattatgga acaatgaccg cgccgttccc acttctgccg cagagcttgc ccgggtggta
    13561 aaatttttca tcgacaacga ccattacggt atctatcacg ccgtatgcag cggcggttcc
    13621 tgcacacgtc tggaatttgc cgaagagatc ctgcgtctcg ccggaaaaga ggatcagctt
    13681 accgtcacgg taaaagatag ggctccggag aaatattccg tgctggataa tatgatgctg
    13741 cgtctcaccg gcatcgaaga acccagtggc tggagagaga cacttgcttg ctacatgaaa
    13801 gaaaccggag gactggaata gtggaaaaac acgaaaaaga aaagaaaaaa ctcggtctgt
    13861 ccaccaaaat ctttatcgcc ctgttaaccg gtgcggtatg tggtgtgctg atccattatt
    13921 atatgccggg agggtacttt agagatacga ttctgatcaa cggtattctt tatgtactgg
    13981 gaaatggttt tatacgcctg atgcagatgc tggtggtccc tctggtattc tgctccctgg
    14041 tctgcggcgc catggccatc ggtgatacga aaacattggg aactgtgggt gtgaaaacga
    14101 tcctgttcta cctgtgcacc actgctttgg cgatctgcgt ggcactgggc attgccagtc
    14161 tgatcaatcc cggcgtaggc ctgaatatcg cgctgccaga agatgccgct gaggttgcca
    14221 ccacacaggt agattttgcg gagacgttgt tgaatattat tccgaaaaat atttttacct
    14281 ctctgtccgc cggagatatg ctgccgatca tcttctttgc tttatttgtc ggtattcttc
    14341 tggctgccat gggcaaccgg gtctccactg tggcgaactt tttcagtcag ttcaatgaca
    14401 ttatgatgga aatgacgatt gcagtcatga aagctgcccc tgtcggtgtc ttctgcctga
    14461 ttgcgaagac cttcgcaggc atcggcttcg acgcttttgt gcctatgctg aaatatatgg
    14521 gaagtgtcat tctggcgctg gcagtacagt gtttcgtggt atatcaggtc atgctgtttg
    14581 tgttcacccg cttgaatcct tttaagttca ttaaaaagtt cttcccggtt atgacctttg
    14641 ccttctccac ttccaccagt aatgcgacga tccctctgtc tatcgacacc ctgtataaaa
    14701 aaatcggtgt ctccaaacag atttcgtcct ttacgattcc tctgggtgcc accattaaca
    14761 tggacggtac ttccatcatg cagggcgtag cagttgtttt catagcacag gcttacggta
    14821 ttcctctgac cccgggtgcc attgctactg tcattgccac cgcgacgatc gcttccatcg
    14881 gtactgcagg tgtccccagt gtcggtctgg tcaccctgtc catggtactg acctccgtgg
    14941 gactgcctac cgaaggaatc gcactgatta tgggtatcga ccgtattctg gacatgctgc
    15001 gtactgccgt aaatatcact ggtgatgctg tatgtaccac catcgttgcc aagcagcagg
    15061 gagcctttaa cgaaaagact ttccgggcag ataactgatc acctgtttac agctttccag
    15121 atggctgcac tgtataaaaa aagtgaaaca ttctgtcttg tgctcaagtg caccgtcaga
    15181 tgtttcactt ttttatagct ctcagctatt ctatttcagt ttattcaatt ttgcccggtt
    15241 ggcaataacg atcagccgca tatcctgtgt cagaggtact tccggatcga tattggtatt
    15301 tacatttttt ccttcgatga tcgccaccac gttaatggaa tattttttcc gcagattcag
    15361 ctcgatcagg ttcttcccga tccactgctg tttgacttcc atttccacca tagacacatc
    15421 ctgggacaat tcgataatgt ccatgaaacc ggagcttaac agatttttgg ccagatggat
    15481 ccctgactca ctctcgggga atactacacg gtctgctcct actttcgtaa ggatcttgca
    15541 gtttatctca gtggcgcatt tggtgataac ggtctttacg cccagttctt tacacagcat
    15601 ggttgccata acactggctt ccagatgatt cgccatggtc acaatgacca catcaatatt
    15661 cgcaataccg atctgtctca acgcttccga atctgtaaca tccgcacatt tacagtatgg
    15721 aatctctgaa attgccgcat tgacaactgc ctctctgttg tccaccgcta ccacctctgc
    15781 gccgttctct gccaactcct tcgccacact tcttccatat cttccaagtc caaatactgc
    15841 ataggtctga ttgattccca tagtctcttc cttcctgttg cttcgatctg ctttatccga
    15901 cgctgatctc ttctgtcggg ttcttcacaa tatttttgtt ctttttctgc gtgctgaatg
    15961 ccactaccag agatatggga ccgactcttc ccagatacat ggtcacgata ataatcagtt
    16021 ttccccaaag attcagcatg gaagtcaggt tcctgcttaa tcccacagta gctgtggcac
    16081 tcactgtttc atacacaata tctatcacat ccgcatctgt cactgcagat aaaagaattg
    16141 tggacaacga cgcaatgata aaaaacatac ctacaaccgc taccgattta ttcaccgcct
    16201 gcttcggaat atttctccca aaaagctccg catcctcctt attacctata gtggcaaaca
    16261 tagataccag cagcactgcg atcgtaactg tcttcattcc tcctgcggtt cccaccggag
    16321 agccgccgat cagcatcaaa agcaccgaaa tgatcgcaga ggtattggtc agatcctctt
    16381 gtggaattgt ggcaaaacct gcggttcgcg tagttactgc ctggaacagg gatgcccaga
    16441 tccgttgcgg caccgtgtaa tcctgcatcg tcagaggatt attgtattca aaaatataaa
    16501 atgccgccgc tcccactaca ataagtattc ctgtcaccgt gagtgctatt ttactgtgca
    16561 gtgtcagcag tcggaaacat ttcagcttct ggctccggat atttttcaaa acccgcagta
    16621 catcccacca gacgatatag cccaaacctc ccaatatgat caacagcatc gtgaccagat
    16681 tgaccatggg ctgaaataca tagccgcaca gactgtcttc cgccataata tccattccgg
    16741 cgttgcagaa ggcagatacg gcattaaaaa tcgaaatcca tatgcctctg agtccgtatc
    16801 ccggcacaaa tactgtcatg tacaaaagag ctccgcatcc ctccaccacc agagtaccta
    16861 tgagaacctt gcggaggaat ttcacgattc cggagatatt attcagatta aatacatcct
    16921 gcagcatcca ccggttaccc agtcccagtc gtctgtgcag tcccatcatc acaccgtaca
    16981 tgaccgtaat gactcccaga cctcccaact ggatcagaaa caggatcacg atctgtcccc
    17041 agacgctcca ggtggaatat gtgggaaccg tcaccagacc ggtcacacat atggatgtgg
    17101 tagccgtaaa taaagcatct atataaggaa ccgcttctcc ggtagctgaa ctgatgggta
    17161 atgccagcag gaagctaccc actaatatgg cacacagaaa gctaagcatg atcttctgtg
    17221 tcgtagacaa ctgtattatt tttttactgt tcataacaca ctccattccg ccgatgcttt
    17281 atctcatcag tggaatcgct aatcaaattt cacgggtgca atccgcaatg ctatgattat
    17341 aacacacttt taaataaaaa actcctattg gagacagttt gttgctgtat ccatcatagg
    17401 agttttcttc tgatcttatt tttcttccct tataccttaa aacgatatcc aacaccccag
    17461 atggtctcga tatattgagg ctttgcagta tctttttcaa ttttctcacg gatcttcttg
    17521 atatgcacgg taaccgtagc gatatcaccg atggaatcca tatcccagat ctcccggaac
    17581 aattcttcct tggtaaatac atggttcgga tgctccgcca ggaacgtcag cagatcgaat
    17641 tccttggtcg taaacgtctt ctccacaccg tccacataga cacgtcttgc agtcttatca
    17701 atacggatac cacggatctc cacgatatca ttctgctgct tctggttagt tcccaccagt
    17761 ctctcataac gggccatgtg cgccttcaca cgggctacca gttcactggg gctgaaaggt
    17821 ttggtcatat agtcatccgc acccagccct aagccgcgga tcttatcaat gtcgtccttc
    17881 ttcgcagata ccatgagaat gggaatattt ttctcctcgc ggatcttctt acatacttcg
    17941 aagccgtcca tgccgggcag catcaggtcc agaatgatca gatcataatc gcctgccagt
    18001 gcggtcttca gtccctcatc gccggtattg cagatatcga ccttgaaatc actgagctcc
    18061 agataatcct tctcaagatc tgcaattgcc acttcatctt caataattaa aatcttactc
    18121 attatttatc ctccatttac agaatgtcct tgtattatcg attatcatgc catttctgtt
    18181 tcattccctt attcattact tggttccacc cggctgcagc tcaatatatt ttctcaatac
    18241 aaaatgcata caggtgcctt ccccttcttt ggctgtcgcc catacataac cgccatgatc
    18301 ctcaacaatt tttttaacaa tggataatcc gatgccactg ccgccctggg cggaattgcg
    18361 cgaagcatcc gtgcggtaga acctctcgaa gatcttctgc agatccttct gtgcgattcc
    18421 cttgccgttg tcctcgatct ctacccggat ggaatccact tcgtcaagaa tacggatatc
    18481 aatatgcccg tcaggcttgt ccatatattt tacgctgtta ctgataatat tattgatgac
    18541 cttcttcatc tgctccggat ccgcgatgac aactgtgtca ggagatacca gattcgaata
    18601 gttcagcttg ataccccgct gctccagatc cagtccgact tcttccacac agtcaccgaa
    18661 ataatccgcc acgttgatcc ggtggaaatt gtacgggatc cggttattat caattccgga
    18721 ataagtggtc agctcgttga tcagacgatc catatcaatg gctttattgt agatggtctt
    18781 gatgtatttg tccatcttct ccggcgtgtc tgccacgccg tccatgattc cttccacata
    18841 acccttaatg gctgtgatag gggtcttcag atcatgggaa atattgctga ccagctcttt
    18901 gttctgcttc tcatgctgaa tgttctcttc tgtggattcc ttcagccgca gacgcatatc
    18961 ctcgtaattg cggtagagat caccgatctc ccccttggca tccgtctcca gaacataatc
    19021 aaagttgcct tccttgatct tgcgcattgc cacattcaac tcattgatag gttcaaatac
    19081 gcccttgtgg atccactggg tcagcatcag gctggtgaag atcaggatca caatgatcgc
    19141 cacgaacata tcgatcagca gatgtctcga gatcagcgaa ttgaccttcg tgacgataaa
    19201 tacgctgccc ggtgttccat cccgaaaggt gaaatcaatc tgttttacat acttctccag
    19261 ttcattgtag aaatatccgc tgtcatccga cagattctcg tcaccatagg caggcagttt
    19321 ttcaaaaatc tgctgtgcgg cttcttcatt gccggcataa tacagcttat caccttttct
    19381 tacaataatg taagtagact tccgcgatac ctcctcattg atatgatcca gatattcttt
    19441 atcttccagc ctgctgctgt cttccttcac ctggtccagc agcacataat acgcctgatc
    19501 cgtggtgttg gtgaattccc tgaaattctc cgacatcatg gaataatcaa tatccgtcag
    19561 gctgagaccc tgagagtaat tcatcaggta aatggcaatc attacaaaag ccatgatcgt
    19621 caacaataac ggaagtaata taatacttat aaatgttaca cgaagtcttg ttttaaattt
    19681 catattactc ttgcctttca gcaacctgca cgtgaataat ttatatttta tactttactt
    19741 tcgctcatta tagcatgatt cccaccttct gtgtataaag gaagcaagaa tatcattaaa
    19801 aaaatataaa caaaattttt tcatttttct gttgacatta ttctcgcatc atgtataata
    19861 agcttataag aaacagtaat cattactatt attgagaaag gggcttatca tggcactgaa
    19921 acgaagcaga caacgggaaa tgatcaaatc ctttctcatg ggacgcaagg atcaccctac
    19981 cgctgatgtg atctattcta atctgaagca gcaggatccg aacttaagtc tcggtaccgt
    20041 ctatcgtaat ctgactctgt tgtcagacat gggagagatt ttacgactcc gggtcggtga
    20101 cggtgttgac cattttgatg ctgatacttc cgaacattat catttcgtgt gtacggaatg
    20161 cggaagcgtc attgatctgg acatggatag tattgattcc atcatggaga cagccggaga
    20221 gagatttgac ggccggatcc ggggacatgt cacttatttc tatggcactt gtcccgcctg
    20281 caccagggtt gctacggagt cctagttttc tgaatgaaaa ctggtttcgt tccataatat
    20341 atttgtgctt ataaagcaca cgcaaaaaga gaaaactgaa aacaaaacat ttcattagag
    20401 aaaaggagaa aaaattatgg caaagtatgt atgtcaggta tgtggttatg tttatgaagg
    20461 tgatgcggct cccgcagagt gccccatctg tcacgctccc gcttccaagt tcaccaagca
    20521 ggaggaaggc aagacctggg ctgctgagca tgtagtaggt gtagcgaaag gtgctcccga
    20581 ggatatcatt gcagatctgc gtgcgaactt ccagggtgaa tgctctgagg ttggtatgta
    20641 tctggcaatg gcaagagttg ctcacagaga aggttatccc gagatcggtc tgtactggga
    20701 gaaggctgct tacgaagaag ctgagcatgc tgcaaaattc gctgagctgt taggtgaagt
    20761 cgtaaccgat tccaccaaga agaatctgga gatgagagta gatgccgaga acggtgctac
    20821 cgcaggtaag tttgatctgg ctaagagagc caaggctctg aacctggatg ctatccatga
    20881 caccgtacac gaaatggctc gtgacgaggc aagacatggc aaggcatttg agggtctgtt
    20941 aaagagatac tttggctaag ctacaagcca tgcaaggata aaataaaaag tcactgggag
    21001 gacatttatg tacttccagt gactttttat tttagattta tacggcgaca gccgtccatg
    21061 agcaagtcac gcagtgactg cgaatgggtt gcatggcgcc agccttagga gcgagccagc
    21121 ccttatacgg ttccggagat caccagcgtg ctgacgccgg ggttgatccc ccacgctcct
    21181 gtaacgggat cctgggtgta agtcgctgcg ggtaccgtaa tacctcctgc agttccggta
    21241 aataatccgg tagtcgtatc atacgtatag tttgttccct ctgcccatgc agttccatta
    21301 aaattcacag tcagattctc caatacggga ttaaaggtat ctgtgatcac gacaccggta
    21361 gctgcatctg ctgccgtatt ccccagattc tgtatcagga acgtatacgt caatgttcca
    21421 ttctctgtca cgggtacggg gctgacggac tttgtgatcg taagaagcgg tccgcttgcc
    21481 gcatttactg tctcattcac agtaacagga gtgactcctg ccccggcaat cgtcgccgta
    21541 ttcgtaatgg aagcctctgc tgccagcggc gcataagagt tcacctcagc ctcataggtt
    21601 agtgtcacat tacctcccgc aggcacagta attcctgtga ttgtcagagg cggtcctgcc
    21661 gttacagccg gtgttgcctg taaaattccg tttgcatagt attttacggt ccccggaata
    21721 tacgtcagag gtgtcagttc tctggtatta aacaaatact ctcccagatt atccgttact
    21781 gtaattccgt taaatgctgt agtgcctgag ttcacaatgc tgatcacata cgtcacattt
    21841 tcattctgcc cgtaggtatc ccgcactgct gtcttggttg ctgacaatac ctccgtgatc
    21901 tcacccactg ctatattgga gtttgtaatc tcatttccat agcgaagctg tgcctggttc
    21961 gtaaatctag ccatatattt ttgtatcctt tcattgtatt tggatttact ataatatatg
    22021 actgttttta agaaagttga cggattctgt caccgcattt cttgactttc ctcttccacc
    22081 tccgtatgat ggaatcacag gcattcactg gcgctgctcc aaaaaacatc gaggattcaa
    22141 catgaagaaa aaagtattta acatcatcca gatcggtgac aaaagtaacc gtctgagccg
    22201 gctctttgat atttttattg ctatcgttat ctgtgcaaat attctggtga cttttctgca
    22261 aacctttgat gagcttgctt ttttatttcc ggtatttcac gtgatcgagg ttatcactat
    22321 tctgatcttc tgtgtggaat atgtcctgcg gatctggact gcggactatc tatatcctga
    22381 taaaaacgag ctccgctccc ggctgcggtt tctgatctcc ttcgacggca ttatcgacct
    22441 gctgacaatt ttaccctttt tcttcctgtc cggtatggtc atcttccgga tgttgcgggt
    22501 ggcgaggatc ttccatctgt tccgcctgaa tgcacggtat gattccttca atgtcatcac
    22561 tacggtatta tatgaaaaac gcaatcagat catttcctcc gtgttcatcg tactgatcct
    22621 gatgctcgcc agcagtctgt gcatgtacag cgtggaacat gattcccagc cggaagtatt
    22681 ccgcaatgct ttcagcggca tctggtggag catgtcaacg ctactcaccg taggctacgg
    22741 tgacatctat cccatcacca cgcttggccg gatcatggcg atctgtatcg cctatctcgg
    22801 tgtcggtgct gtggctatcc ccaccggtat catcagtgcc ggtttcgtgg aacagtacca
    22861 gcgcaaaagc agtctctcta atattaaaga agcagatatt catgagatcg ccgagatttt
    22921 cgtagataaa cgctttgcgg gcaaatccgt ggaagaaatg gaagaggcag aacaggtcac
    22981 gatcttcctc attctgcggg aagatctgtc cattctgcct caaaaagata cgatgttgaa
    23041 attaaatgac atcattgtca ttcgtggtga gaatccctaa caatagtgaa gactgcctct
    23101 tttgcagggg cagtctttat tatttgtgat acttatttgg cttccacggt ttccgtagtt
    23161 tctgttgcca ctgtttcact ttctgcggtt tcagcctcga cattctcggt ctccatcgca
    23221 gtttccgttg tctcgctttc cgttatcgta gtatctgcag cttcggtgct ttctgtctct
    23281 tcagcagtct cctccacctc caccagatcc tccagtgtat aattgaagcc tgtcaccacg
    23341 ttggtggcca cgacatacac tttcatttca gagggaaatg caatgtaata ctggctggtc
    23401 atggaattca gattaccgac attaatgatc acgctgcgat ctgcagtctc gtactgaatg
    23461 gtacgctccg gatccgccag tccgtactgt gacatatccg tcacattctc aatcacctga
    23521 tctgccttta acggtgccac cttcaggatc atcgccttga tacgctcctg gttcagattc
    23581 agggaatgat cgtccgcata gtaccatgtg tcatcctctt tttcaaaggc ataggtctct
    23641 ccctcatatg catagcggaa gctgataata tcatcggagt tcacatccaa caccgtctcc
    23701 tgtgtatcct cggtggtaga cgtcgcagaa gatgcatttt tattatactg gcggatgccc
    23761 aggaatgctg ccgccagcac caccagtgcc agcagcattc caatcatctg tctttgtttc
    23821 tttttcatat ctataaactc acgcctttca caattatttc ttccgtctgc ggaaccagat
    23881 cacgaatccg gcgatcatac agcccacggg aagaatcacg gtaaccagca gccccaacaa
    23941 cagaatctgt gcggaatcca ctaccaaatt agagacctca tagcttttta ccggaataga
    24001 taccgaggtc tcatgatctg ccattccact gaaagtattg gtgaacaggg tcaggttcgc
    24061 accgctgacg acagagttgg catcgtcggt gaacagctgc tcacaaccgt acacgaccag
    24121 tgtcgcatct ccatcatcca gtgtctttac tgcctcaaca cctaatgcga agggaccatt
    24181 catatcattt tcactctgtg aaaaatcctg tgtattgttg acatcctgtt ttgcaaaaga
    24241 gctgtcggag gtgctcagga atacatcata ggtaatatcg ccggtggctg tctcgtcggt
    24301 acttacttcc tccgggacga tgatgccctg agaatacggc aggaacagat attttccgta
    24361 ggtaccggag gtatagctgt cagagctctg tgtcggcaga atataatacg ggcttctgta
    24421 gtagccgttg gaatcattct ccactaccag tcctttggca atgctcagat tcatgtagct
    24481 taagatagca tctatattgg gtgtctcctc atcggtataa ccggttacca ggatgacttt
    24541 tccaccgttg ttcagatagt cgatcacctt atccttatca tcggatgaaa aatcgctggt
    24601 ggcaccgtta atgaacagac aggccgcatc gtcagggata gcatccagat ccatcagatt
    24661 tacggtctca taatccacat tttctttgtt cagtgcggat gtaaaagtgt tgcttaaact
    24721 cagttcatta tgtcctgttg tcatatatac cttgggcata tcatcattta acacataatc
    24781 cagcgcactg gtgatctgtc cttcgccatc ataaccggtg gtcgtggtgt tataactgta
    24841 cgtcgaatag tcaaaatcat aagaagactc atacacatcg ttgtaatcaa tgaccttgct
    24901 gcgcttgtcg ctgaccacga tcaggctgtt ggtgctgatg ttgccggtgg tgtactgggt
    24961 gtagaacatc gggttaacgg tgggatccac atattccacg gtgatatggt cggacagatc
    25021 atcataccgc tgcagggtct ggcccagcgt ggtatcctga ttgtctttat ttaccaacac
    25081 atagattgtc acatcatcct gcatattttt cacatagtcg acggtctgat ccgtcaggga
    25141 atacagcttc tggctggtca tgtcgatagc tgtccagccg gaaggcatct cgcccatgat
    25201 aatattaacg actactacca gcgctacagc tacagcgatc ataccggtac tgtaagcact
    25261 gaagcttaag tttttcacgg acatgctgta acggcgtttc tgaatggact gtaccgtcag
    25321 gaacagaacc agtgccgtaa cggatacata atatacgata gaagatacat ccagtgtgcc
    25381 atttaacagg ttggaaaatg gtgtataaag gtcaaagcaa cgtagcagct ttgtcaacag
    25441 attgccggtg ctggaaataa tggagcagat ggaatccatc atatatccca ggaacaatac
    25501 taaaaaagta agtactgctg caatgacctg gctctccgtc agggaggaca ggaacaggcc
    25561 gatggcaatg gcggtcatcc cgaagaggaa atatgccagg atcgacagat aggcctctcc
    25621 catgggaaca ctgccgtact gcgccatgat cagcgggtaa aagcagatga tcgctacggg
    25681 aatgacaaat atagttaaca gagccaggaa ttttcccata acgataccgc ctacggacac
    25741 cggtgctgtc aggatcagct gatccgtctt gctccgcttt tcctccgcca gaatacgcat
    25801 agtcaggatc ggcacggtca gcatgaatag aaaggtaacg ctggatacca catacgcaaa
    25861 ataaggatat ccgttcatca ggttataaac gctgaaatac agtcccagga aaaacagggt
    25921 gactgcaata aataaaaatc cgataaagga acggaaatag gatttcaatt ctctcttata
    25981 aatggctagc atatgattcc gcctcctttg tagttacagt ttcttttttc tcttctccgg
    26041 gctgtgcttc ctcttccgaa gcttctgtct gctgtgcggc agcatctgac atttccggcc
    26101 tttttctttt tgagaatagt ttgtgcttct ccggctgtgt gggtgctaca tcctcggtca
    26161 gttccaggaa gatgtcttcc agagacttct ccgcatgggt catctctaaa ataggcagct
    26221 ttgcatctgc cagtgcataa aatacctgct ctctgatatc tgcattctct ttggagatca
    26281 ctttcagctt tgcaatattt ttgggagtct cagtattttc ttcgatccgg tcgatctgtg
    26341 agatctcctg caatgcactc ttcaccgcag cggcgcttcc ctttacttcc agatccagtt
    26401 ccatggaacc actcataagc ttctgcagat tctccggaga atcactggct accagcttac
    26461 cgtgggctat gatcataata tggtcgcaga ccgcactgac ctccgacaga atatgggagc
    26521 tgaggatcac ggtatgattc tcccccagct tgcggatcag atcacggatc tcgatgatct
    26581 gtttcggatc cagacctacc gtgggctcat ccagaatgat aacctcaggg tagccaagga
    26641 ttgcctgcgc cagtccgact ctctgcctgt aaccttttga cagattcctg atcagacgct
    26701 gctgcatatc ggtgatctgt gtcatttcca tgacctcatc aatctgctgc ttccgctctt
    26761 ttttcggcac ttttttcagt tctgccacga aatccaggta ttctctgaca gtcatgtcaa
    26821 catacagcgg cggaagctcc ggcagataac cgatggcacg ctttgcctcc tccggatctt
    26881 tttggacatc cttgccatcg atcgtcacag taccctccgt ggctgccaga taacccgtga
    26941 tgatgttcat ggtcgtggat ttacctgcgc cgtttggacc aagaaagccg taaatctgtc
    27001 ccttttccac acggaaagag agatgatcta cggcaatatg gtcaccatat ttttttgtaa
    27061 gattgcttac ttctatcata tttgcctccc ggatatgatg tgataccccc acctgatatc
    27121 tacgggtacc cgtaactgat acccacaggt acccagaact gataccccga attgcttcgc
    27181 agcaagctgc tcccgcaatt ctaaaaacat tcggaatccg ctgatttgct acgcaaatcg
    27241 ctacttcctc atgttttacg ggtgtaaaat tcaaccgctt tatcatgcaa gcatgaacag
    27301 cggttaacct ttacaccctt gtatcagtat atgtctcaaa tgtcatgaaa aggtgaaaca
    27361 tttgtgaaaa atctgtgaag ccgcaaccct taaagcttcg tcacggtcag cgaaatgctt
    27421 aaatttcctg cctccgccgc cagatgcagc ggcagcttcg ccacaggaat atcttcaaaa
    27481 atgaattccg gatcaccgga tgcggccgac tggaataaga aaagtacccc ctccacatag
    27541 agcaccactt ttttctgatt gctgtaaatt gtcagggaga ccagcccgtt cttctgacga
    27601 tgcagggtag aaagtgccgg atagagcacc ggttcgctgc tccacttcgc ctgataatag
    27661 tagtatcgct ctgtcagtgt cctgccgtcc gccgccagga gtgcttggca tgtcgtttct
    27721 ccgggtaatc cacggtacac tgggagattt tccggtctta tccacaggtc cccagtgagg
    27781 aatccacgct ttctgcacag gctgtaaaac gttctcactt cggagcagtt caccccctct
    27841 gccccggtcc cggttccttc cagccggatc gcatttaccc ccatccggac cagccgttcc
    27901 agatctcttc gtacctgctc caccctcgtc ccggcatcac tgatctgcgg gggaagccgg
    27961 tagactactt catgtggcaa aaactctttt tcattgagaa agattccctt tttttctacg
    28021 acacgcacgt cataaatggt cagttcctgc cgccaataca tttccacttc cccggttata
    28081 tcctcagcat gatctgattc ttcctcgttc tttgtacccc ccatattttc tccatactcc
    28141 acggttccga accttccctg tgccttcacc agatagacat agggatcccg catgccgtcc
    28201 cagagatgcg gccgcaacag caacgccgtc atggaatccg cttcttctaa tgagtattcc
    28261 ccatgaaaaa caaggcgttc gccctccgca ccttcccaga tctcaatctg taaagcggac
    28321 agcaaacgcc ctatcttttc cggcagggca aattccagat aagccttgcc attttccagt
    28381 tttgttgtaa ttccaagatc gtctctcatc tgcatttctc tttcctgcct ttcgcattct
    28441 gttactaaga gtttttgcag ataacatttt atttatgccg gaaatccatc tttcccgcac
    28501 actattatta catgggacaa gacctgcggc cgggcacttc ctctccggat acataacctg
    28561 catgaaattc catgttgatg tcccgaagct ccttcttccc gtcgatgtat gcctgataca
    28621 tttccgccag tcccgaagta cagccatcac agttggcttc catatatccc agagactccg
    28681 ccacgatctg ctcccgctct tcttttgtgg tatccttaat cagatatcct gccattttct
    28741 tttcctctct tgtatttctt ttacagccaa tgtatcacag caatctgccg ggtgcaagtt
    28801 tttctctgca tttagagtaa tcgaaaatct acgaccgaag aattctctca tagatctgca
    28861 aatcctcatt cttaaacaca ttaaagatca catttttgac actgctgtca ggagtctcct
    28921 ccaaatactc ctgtaccgtc tgtacggcaa tctgcgccgc cttgtcgttc ggaaagcaga
    28981 ataccccagt ggatataccg cagaacgcca cactttccag cccgtatgcc gatgccagtt
    29041 ccagacagga caagtaacag gaccgaagca atgcgcaatg tttctccgtc agctttccct
    29101 gcacaatagg acctacggta tgcaggacat aacggcaggg cagattgtag gctttcgtga
    29161 tcttcgcctg tccggtgggt tcctcatgcc cctgttcttc catcatccgg gcgcagtcca
    29221 gtcgcagctg cacccccgca aagctgtgga tgcagttgtc gatgcactta tgattcggca
    29281 cataacatcc ggtcattcca ctgttggtgg cattgacgat ggcatcgcat ttcagggtca
    29341 tgatatcccc ggctgccggg atccgtaaag caggcacaat aaagtgcccc ggttacccaa
    29401 aagatactat tgggacaaac atataaacct gcaattgaaa tatttttacg tttttgtaac
    29461 aataggaaca ggaaccttcg ttatgactca ttgaaaggcc tgggaacaac aaatgatata
    29521 tctgatgatg atcgacgccg aagaagacaa acaaaagttt gcgattctct atgaaaccta
    29581 tcggcacctt atgatgaaag tagctctgaa tgtgctgaag gatacttttc ttgcggaaga
    29641 tgccgtacat gaggcgttta taaaaattgc gaaaaacatg gaaaaaatag gggaaatcga
    29701 cgccacggcg acgaaacgct acctgcttac cataacgaaa aatgcgacta tagctattta
    29761 ccggaagcga aaccatcaat ttcaacggga aatctatgtg gatgagctgg aagaatgtaa
    29821 tgtcccggtg acttatgcag agacggatgt ggatgacgga atactgtaca tccttaggtg
    29881 cctgcccgta aaataccggg atgtatttct cctcaaatac tccggcaata tggacaacga
    29941 tgaaatcgca aaagtattaa acatcaccga aggaacgatc cggcagcgat tatcccgggg
    30001 aaagattatg atacaggaag ctttgaataa acgggaggaa aataatgaga tccatggaag
    30061 taacggatga atggctctat cagtatatgc ctgtggtgga caatgccatg ataaaagcat
    30121 tggaaggtca ggttgatgaa gactatgtat tttcaaaacg atttgaacgg aaaatgaaaa
    30181 aagtgatccg tcacgaagcc catccgatgc tgaccggtgc gcaacagctc ctgaaacgcg
    30241 tggcaatctt tttcacaggt atcgtatgcg ccgggctggt tctgacgtta agtgtggatg
    30301 cttatcggga taagtttttc atgacactaa agaccctgtg cgaggatgct acgcagtata
    30361 actactcttc cgaggaaaaa gatgcggtac tcactcccac gataccgacc tgcatcccgg
    30421 agggctatac agagacagac cgaagagaca gtcctctttt ttgtgaaatt ttctatgaaa
    30481 acaatgccgg agaacagctc atctgggagc aaatgctaat ttccgcgaat aatgccctga
    30541 cactcgtatt agacaatgaa tacgatgccg aagaggaacg tgtcatcggc ggtcacattg
    30601 ccacggtata tacttatgtg gatggttacg ccatggctta ttatgaagct gacaattatg
    30661 tgtacgtgat caccgcagat cacatgacca ccgaggaaat gtttcagctc attgccagta
    30721 tggatatctg attgtataat aaaataaaaa ggaggcaaga accataatgc gaaataaaaa
    30781 attaagaacc gttttctgct gcattctgat ttttctgctc gtatttgcct tattttgcct
    30841 tggagactac gggatccgct attatcatac tcccatcaaa aaggattatg actataccgg
    30901 aaccggaatg ctggtcagtc tgacggaacc ggataccttc gaacaggtac cggtggagat
    30961 ccacgggaaa accttacatt atctctgggg gaatcaggaa gacgccatag cgggaaatgt
    31021 ctggatgaac ggagagaggc ttttcgtgga aggtgcggat gacatgggat tctatgcacc
    31081 ttttatctat gaagaaccct attactcctg cctgaccgaa tctgtaacaa atatcccggg
    31141 aaatatgttt cttatctccc gggatctgaa aaccgtattc tgcggtgtac ttatggacgg
    31201 gaaagaattt ctgctgataa taccggcaga aaataaatcg gatgccgacc gtaccctgaa
    31261 atccggtctg gaacagtccc agcctctttc ccgttggctg agtgactatc ccttattcct
    31321 gtaactgtcc gtccacaata cgcacgatac gtttcgcaga ctgtgctacg cccaggtcgt
    31381 gggtgatcat gacaatggtg ttgcccatat cgtttagttc ccggaacagc ttcagtactt
    31441 ccttgccgct ggtctgatcc agagcaccgg taggctcatc cgccagaagg atcgccggct
    31501 gtcccaccag tgctctggca atggatactc tctgcttctg tccgccggac aattcgttgg
    31561 gcttatgtcc gatacgatga tccagcccca gcagtttgat agtattttta cacttttctt
    31621 ctgcttcctc atgagtcatg ccacgcatca gcaggggcat gataatattc tgcatgacat
    31681 tataggtcgg gatcagctga tagttctgga aaatgaaccc gatcttctgg ttgcgtacca
    31741 tggtcagatc gtcctccttg gtcttgtgga ttgccatgcc atccagataa tattccccac
    31801 tgtccatcac atccatacag ccgatcatat tcattaacgt agtcttaccg gaaccggaag
    31861 ggcctaagat tgccacatat tctcccttat ttacctgaaa attgatgtcc ttcaaaatcg
    31921 gcagacgctc ctctcccagc atatagcctt tatttatatt tttcatggtg atcattgcgt
    31981 tttccatcat aatccttaca cctttcgcac tactcctgat ttaaagcttc tacggggatc
    32041 agtctcgatg ccttatatgc cggatagaag ccgaagatgc ttccggtgag gacaccaaac
    32101 aacagcgaca atactgcacc ctgtaccgac agggacactc tcacgttgaa cagctgcacg
    32161 aatggcgtaa ttccaagagc caccagcacc cccagtacgg ctcctaccag actggtaaag
    32221 cttgcctcca ataaaaactc caacaggata tccttccggg aacagcccag tgctttcaaa
    32281 ataccgatct cgttggtacg ctccttgatg gatacaaaca gcacgttcat aataccgata
    32341 ccgccaacga taaatacgat cactgccatg gagatcagca gtaatgtcaa agtctcattg
    32401 gaagcggagg ctgcctccat cttgcttccg gcatcgctga tggtaaatgt ggtattggga
    32461 tagctctctt ttaaggcact ctccacatcc gtcattacgg tatccacctg attcacatcc
    32521 tccgcaatga ccgtgatggt tggactgata ttcgtaccgg taatgtattt gatgccggtt
    32581 tcgtaaggta tatagatcgc cgtatccgga ctgatgccgg atgccacagt tcccatctcg
    32641 gatagtaccc ctgatactat ataggggcgg ccatcaatat agactatacc gtcataagca
    32701 tcgtaggcgc tgccaaaaat ttcctttgcc acggtggcac ccagtacgca caccttttcc
    32761 ttgctctcgt tgttttcttc tgtgaggaaa tccccgatgg ccatttccag attactgatg
    32821 gcagcataat tgtcctccac accggcaatg gtgtaatagg aagcagaggt catatcgcct
    32881 ccctccaccg atgctgtagt ggaataagag accgtagttg cagagattcc cgatacaaaa
    32941 gtcgccagat catcacagtc ttcactggtc aatgtcacgg cttcgctctc ttcctcctgt
    33001 ccgaatccga aaaatccgcc gaaatccatg ccaccgccgg gaccaccaga cgtaccgccg
    33061 ccggaacctc ctccgggtcc tccgcccata ttggtgacgg taacgcttcc tccaccgccc
    33121 ggcatttcaa agctaaaacc accactgtca ctgctgtcac aggaaatatc aatagctccg
    33181 gcattcagat tcttgaactg ctccgccact tcttcttttc cgccggtacc gattgccaat
    33241 accagcataa tagtggcaga tcctacaacg ataccgatgg aagtcaggat cactttgaat
    33301 ttattctgtg acaggttcag ccacaccaga cgcaatattt cggataatct catattctac
    33361 ttcttccctt ccattgacct tactttccca caggtctccg ctctacttcg taatgctgct
    33421 ttcgatcaat acggtatctc cttctgcaat atctccggag atctccacgt tagaactatt
    33481 tgacagtcct gtcgttattt cctttttcac aatgctgccc tctgctgtct tcacttttac
    33541 ataagtcttg tcatcctctg tgataatggc tctgcgtgag acatacagca cttcttccgt
    33601 ttgttccgta acgaaagtca catctgcggt catgccctgg aacagtcctg aaacatcccc
    33661 ttctaatgtg acagttacgt cataagttat gtttccactg gaatccgatg tcgcatctcc
    33721 gataaccgtc acctttgccg taaagatatc ttccggatat gccgtcaatg caatattcgc
    33781 cgcagatccc agagagatat ccgtcatgtc atcttcatcc actgttaccg tcatggtcac
    33841 ttcatccata tcatacaaag tgaccagaga ggttccggtg ctgatcttgt ctcctgcttc
    33901 cagttccaca ctggtgatca caccgttgta tctggcgcat accgcgttgc catcgatata
    33961 ggtgctgaac tcgttccatt tttccagagt ctcatcgtag gtttcctgct gatcggacag
    34021 atcctgttcc agatacccca gcgcaatatc gtaagtctcc tgtgcggtat tgtaagccag
    34081 cgtccgcagt ttttttgtct cggctgcggt cagttttccc tgctccagtt cccgctttgc
    34141 tttattataa gcggtctcgg ctgtggtcac attttcttct gcctgctcta ccttctgttc
    34201 cagttcttcc ttctgctgtt ccagagagtc cgcagtagac tgtgcctgtg tcgtcagctg
    34261 tgcaaaatag acatatccat aggtatcatc tgccggatcc gtattgttca gactgtattg
    34321 gcagttggat aatacctgtg ccgcatcttc gtaagcagtt gttacttcct ccagctgtgc
    34381 ctgataatct tccagactgg tctgcgcctg ctccaatgcg cttgctgcag aatctaccgc
    34441 attctgtagc tcctgcagtg tggtgctgta ttcggtatca atataactgc cataggcagt
    34501 gctgctgtca taagtatatt ttgcctgctg tctggacagg gtctggtcag cctctacggc
    34561 ctccagatct gcttttgctt tctcaacgtt agactgcagt tcgtcttcca ggtcggagac
    34621 actgtcttct tccagcaggt acaggggatc tccctcggac acctgctgcc ccacggagac
    34681 cactacctct gccacggtca gggtagaatc gtcaccattg ctgccggagc tgctgccgcc
    34741 catattgaag atctgaccaa acatgtctaa gcctccctga gctccgccgc cggaagcact
    34801 catgccgcca aaaccaccgg aaccagagct gctgccggca gtactaccgg aggagccgcc
    34861 ggaggaactg ctgccggaag tatccgttct ctgcagggca ctcatatcca gatcaaaggt
    34921 ctgatccacc gtaccgatgt ccacggaacc gctctccgtc acacctttga ccagtgtacc
    34981 gtattttacc gtggtctctt tataaatgac ttccgtgtcg gaaccaccgg agagaccaat
    35041 ggcaccagcc actacagcga tcacacagac accacagaca cctgtggtga gcttgcgtct
    35101 tttcgaccag cttttccatg atccaatccg gcgcatttga tttcttatat tttctttgat
    35161 ctttttccca ttttcattct gcttcataac tgttcccaat cattcctgtc tgtttctcaa
    35221 catttgctcc atccgcttga tttattgtcc tccgggaccg cgcggcattc cgccgccgaa
    35281 gctgccggaa ttaccacccc cgggaccacc ctggggcatg tcaccgcctc ccggcatctg
    35341 catatcctcc gacatctcga actccgtatc actgccgact gcggtatcag aaccgctgcc
    35401 ggaatcggca gtctcctccg tactcttgac ttctgcctcg gagctgacct tacttgccag
    35461 gtagatggta tcgccctctt ccagtcctga caggatctca atgtccacac cgttggtaat
    35521 gccggtctct acctccgcca attccctgcc acccagtgct gtcttacgat agacataggt
    35581 cttgccattc tcttctacga tcgccttacg ggatacatag aggacatctt ctttttcttc
    35641 ggtaacgaag atcacatctg ccgtcatgcc gccgtagagc tgctccagtt caccttccac
    35701 gctgatctcc acagtgtagc ttaccgtgtt gctgtaatcg gaagtagccg tggtattgat
    35761 ggagaggatg cttccggtat aggaatcctc cgggtatgcc gtaaaggtga tatctaccgc
    35821 atctccaacc tgcaggtcca caatatcctc ctgggtcaca tccacggaga ttctcatgtc
    35881 atccgacgtt gcataggaaa acagagctcc tgtcgtcgtc agacggtctc ccgcttcgta
    35941 gctgacctct gtgatcacac catcttctgt ggcatacagg ataccatcac ttccgacaaa
    36001 atcctcaaac gcctgtaact gttcttcccg tttttctttc gtctcttccg cttcctgtaa
    36061 ggtctccttc agatcttcca cagcagcgtt gtaagtggtc tgggcattct gcccggtgat
    36121 caccgcttcc tgataggtct cttctgtatc cagcttatcg attttttgct ttgcctgtgc
    36181 cgctgccagc tggttctgca gatcggtaat agcactttca ttctccgata aagcatcctc
    36241 tgcctgcgtc aacgctgatt ttgcctgctg gtatttggat ctggcactga gataaccatt
    36301 ctggatcgtc ataaaattca ctgtattatc ggttccggcg ccttcataac cttcttttgc
    36361 cgcttcataa tcttccaatg catcctgata atcttcctgg gcttctgtca gcttctcctg
    36421 cagagaagct gtattcgcct gcttctgctc cagctgtacc tgcagagatg ccactttgtt
    36481 cgtcacgctg gtcccggaag tattgcggat ggatgccgca taggaagcac tgatcttctg
    36541 ggtatcgtag tcggtctggg cttccagcag ggacaattca taggtactct ctgcctccgc
    36601 atagtctgcc tgcgcctcca ctaccgcatt ttgcaacagc gcccgaaccg cctccacgct
    36661 gtcttccgtg aatttcagca gttcctcgcc ttcggtgaca cgctgcccgc tggcagcata
    36721 gatctcctct atcttcagat atttctgtac ggtatcatcg tcatcttcct cactgtcatc
    36781 ctcgtcatcg tcactgacgt ccagattcag atcgtaatct atggattttg tgttgtattc
    36841 cagagagccg ctctcagtaa cacctacctt taaagtacca cgctccaccg tctcttcttt
    36901 gtagatccac tgttctttct gaagtaacgg tgcaataaac acggtatagc aggctgccag
    36961 gatcagcaga agtaatgcga tccccgccag gatcagtttt ttctgctttt tcgtgaggga
    37021 tttcagttta tcaaaatgtg ggccttttaa tttcatgact gtgtctgtcc tccctgctgt
    37081 tcatttgcat tttccgtaga ttccccgttg tcggagccgc ctttcggcat gccaccgttt
    37141 tccggcactt ctccgctgcc cgggactcct tccggcatgc caccgttttc cggcacttct
    37201 ccgttacccg gggctccttc cggcatctca ccattttcca gcttttcgcc gcctccggga
    37261 ccgccctgcg gcatcgcatc gccgtcggac acatcgtcag gtctgccctg catactctgt
    37321 ctctgctgta actgcttgat ctcttcctcc gtcatgccga agactacggt gacggattcg
    37381 ttggcactta agctgtcctc ctcatcttcc acgttgatct gtaccgtcac gctgtatatg
    37441 acactggtac ggctgtcaga cgaggatacc ggatcaatgg ctgtgatcac accggtatat
    37501 ccgctgcctg cggaggactg cacataagca ctgtcgccta cgatcagttt tgcaatatcc
    37561 gactgatcta ccgatacggt gaccgtcagc tccttcggat tgctgtaaac gaataccact
    37621 gcatcggaag tcagagtctg ctccgcccgc accatgctcc gcaggatcgt accatcctca
    37681 gatgcataga aatatccgtc ccccacgctg ctttcaaaca gttccagatt ctccttggca
    37741 tcttcataag tacttttcag agctgccaga tcggacttgg ctttttccac cgtagtatta
    37801 taatcactct ctgcccgttc tgcgccggac tgggaagtct cataggtcag ctttgcctgc
    37861 gccagctgga tctcataatc ttccttcgcc tccgtgactg cctgctgaag gctgggaagc
    37921 ttcaactgca atgtctgtaa gttaaaagaa gcattggtga ctgcatcctc atatttttcc
    37981 tgcgcttctt ccagatcctt caggttctgt tccaaaatgc tgtagaggga tgccagagtt
    38041 ttcacatttg aggaattgct gtcctgtcca ctgctgccca gcacattgcc tgcggttcca
    38101 cccgcttgca cagatccggc tgacccaccg gtgatctggg accagctgat gccccactca
    38161 tccagtttat ccgtcagtgc tttcaggttc tcatcataga ttgcctgata ctcatccacc
    38221 ttgaaataac tcttatagga accgtcattc acataggatt catactctgc tatatcatcc
    38281 tcggcatcct gtaattcctc ttctgccctg gtcacagcac tctgcagact tgctacggtc
    38341 tcatcataaa cgtccttcgc ctgctgtccg gtgaggatgg cactgtctct cgtatactcc
    38401 gctgtgatca ggttctgctc atattcgata gctccggtgc gatacgcaag ctccgcatcc
    38461 tccagcgctc tttccagttc ctttctggct tcctccacac tgtcctccgt aagctttaaa
    38521 atcttatctc ctgcagttat ctcttccccg gaggccacat agacttcctc gatctttagc
    38581 tctgtcgtca gattttccac atcgaaggtc tcctccgtga taccgacatt ggtaacacct
    38641 gacgtagcag tcatatcctc cgtgaagctc agccccgcca tattacccgg catggataat
    38701 tctgaccgat tgccgtttct atgtaatacc accactgctg ccagagaacc gccacccagc
    38761 gtaagtacca gtaccagcgc aacgatgatc cattttttgt ttttcttttg ggaaaaaatt
    38821 atctgcttga tctctttgtt cttcacggca aacctccgat tgctattccg tccaaattcc
    38881 attgttttct caagtatagt tggattttgt attaaaaaag tgttcactct gtgaaaaatc
    38941 tgtgtcctgt agaagctttc cccctgcctg cttctatgca attgcacaaa aagaaggccc
    39001 tgttagatgc tgctttcgca aaagtctcta acatgacctt ctatcataag cttacgaatt
    39061 cgtgctatcg cgctaatatg cccggaaaaa caaatcaccg cttccgggag atgcagatat
    39121 cctccagatt ctgcgcgccc tccggtccga tgggtctgcc gcccgtggtg atgccccatc
    39181 ctgcacggat cttgataata tcgtcgatct gtccctggga caacaacgtc ttcttcccta
    39241 agataaagtt gttcatcatc aggatcttcg cataatattc cgtggtctcc atgcggtaat
    39301 atgcctgcat gagattgtct ccccaggtca gcgcgccatg gtttgctaac agtactgcat
    39361 tgtattcccg acaataggga gctaccgctt ccggcacttc ctctgtgccc ggcagggcaa
    39421 aatgcactaa cggcacacag cctaaggaca gaaccccttc catcagcacc ggctcctcca
    39481 gactgatgcc cgcacaggca aacgccgtcg ccgtcagagg atgtgcatgt accacgccct
    39541 gtactttcgg gttctctttg aatactctga ggtgcatctt gatctcgctg gaaggcttgt
    39601 gcgttccctc caacacattc ccatccagat ccagcttcac cagcatatcc tctgtcataa
    39661 atcccttaga aactcccgta ggtgttgccc agatctcatt atctccgact ttacaggaga
    39721 tattcccgtc gttcgctgcc acgaagccgc cctgatacat cctgcgtcct acctctaaga
    39781 tcatctgttt tgcctgtgca tctgatatat attccatact aatctccatt ccgcagacga
    39841 ttccttccat cggaggaatc cgccttttcc gttccggcat gcaataatat attaccttat
    39901 tgcccttctc cttgtccagc tgatattccc aataattttt tatgcatgct tcctaacata
    39961 ttctttcgta actaattatc tttccctagg tattgtacca catttctatt cttttttatg
    40021 atactaaaat acttatgacc cgccccgata tgagcataga agtaggatga taatccctcg
    40081 atatgcgagt agagggtagg gtgccatgtg ccagtggcac ctgtttagca cggaccgaag
    40141 cggaatgaaa tatcttctca tgtctataca aaaaaacgtc aaaatgctat aattaaattt
    40201 gaattatttt gccaacacat acgaaggaag catagcacta tgacacggaa taaaattaaa
    40261 ttttctcata ccaaatggct gctgccgtta tggatacttc ttatcggcag tattgtctta
    40321 tatatgatcg ctcatgcggt acagcaagac gacttcaggc acatccgcac tcttgcggaa
    40381 ctcaatgctg taacctacgg tgataacatg atcgcagatc tgtatgccgg tatcagtatc
    40441 acggatacac tggagcagct tctgatcagt acggacggca gaattgacaa atttgatacc
    40501 attgcagaca gaatgatggc agattacgtc caaagtatcc agctggctcc cggaggtgtc
    40561 gtaacagata tctactccac tgaaggaaat gaagcaggta aaatagatct gatccacgac
    40621 aaagaccgcg gagagatcgt aaattatgga attgatcatg atctgcttgt catgcacggg
    40681 ccttttgaac tgaagcaggg aggttacggc attgctatcc gcaatcctgt ttttttacaa
    40741 gacgaaaacg gcaattctta tttttgggga ctgactattg ttatcatcaa agctccggaa
    40801 attttcaggg actctgttga ttctttgtcc ggtttcggtt atcaataccg gctatcgaag
    40861 accctctccc ctctgaccga tgaatatgcc gtggtagacc agtcagagga gacgttgacg
    40921 gatcccgttg cttatgattt tactctgggt ggatgtaact ggaggctgga agtcatgccc
    40981 accggtggct ggaagaatgg cacactttta caactgatag tattatgtgg cattgcagtg
    41041 atcctactac tggtggttct aacgatatct atactattcg ttgacgagca acgcaaaaaa
    41101 tttcgtagac tctctctgac ggattctatg accggtctct taaatcgtaa cggatttaat
    41161 ttgcagctgg aggagtatct ggagggaaac aagcagaaaa actgtgtcgg tatattactt
    41221 gacgtagata atttcaaatt catcaatgat gtctatggtc atactatcgg tgatcaggtt
    41281 ctattgcagc tttcacaatc cttagtgcag gcttttccgg ataattccat tattgccaga
    41341 aatggcggtg atgagttctg tattatcctg aaagattgca gtgcagaaga ggctgcccca
    41401 atgatagacg ctttcagcaa ggcttcccgc tctttttctg ccaagggtgc cgaacacaac
    41461 tatagtattt ccctgggcta tgcggaatac cctgccaacg cagaaaaagt ctctgacatt
    41521 ctccgttatg ccgacattgc actctatgag gtaaaattac agggaaaaca tggtgctctg
    41581 gcctaccggc cggatttcca caattcgaaa cggacacagc ttggtttttc attatcggat
    41641 atttctgaca acctgccggg tgcctttttc atctaccggg ccgaaaagga ggatgaacgc
    41701 atcctgtatg ctaatcagga aatgcttcag ctgacaggat gtaaggatct ggatgatttt
    41761 atgcatttta cgaaacatca attccgtaat ctggtgcatc ctgaagatct gacacaggta
    41821 gaagaaagca tctggcagca gatagaatcc ggcatgaacg gatacaatga ttatgtcaaa
    41881 taccgacttg ccgtcaaaga tggaacttat aagacggttc tggattatgg gcgtatcgtg
    41941 gagagtgaac attatggctc ggttttctat gtattggtag tggattgcga atttatcaaa
    42001 acacactacg atgattaatc ataaaaataa aagaataaca aactttcctc cggggcatac
    42061 tggttccgga ggaattttta tatgaaagat cagattaagt cttacttaca acgtttgaat
    42121 cacggagaag ctctggaaaa tgtccaggcg gatttcgtcc gggaatgtaa ggatgtggat
    42181 cctgccgaga ttatgcatgc cgagcaggag ctgctgcggg agggtacttc catcgaagaa
    42241 ttgcagcggc tctgtgatgt gcattccgcc ctgttccatg gttccacgac agaagagaaa
    42301 attgccaatg ccgaaaaaac aacagtccat tctccagctg cccagaaact acactctaaa
    42361 gacataaagg accaacggct ctccgccgct gccgccctga ttcagatctc cgggcatccc
    42421 ctgcagactt ttacgctgga aaatgcagca ctggaaaagc tgattgcccg tgcgaggcag
    42481 gcattggaaa ataatggaat ttccaatgaa ctgcttgacg agctgcgcca gattgctatc
    42541 cactatgcca gaaagggaga cttactttat ccacatttga aggtacaata tgggatcagc
    42601 ggcccctctg cagtcatgtg gacagtggat gatgagatcc gggatgaact gaatgcgctt
    42661 gcaaaacaaa aacaccaaaa agaaaccgat gcatggaaac aacgtttgaa tgccgtatta
    42721 aaccgcatgg aagaaatgat ctacaaggaa tccaatatcc tcttccccaa ctgcgcactg
    42781 aatttctcgg aggaggaatg gtatcacatc taccgggatg ccaaagatta tgcggaatgc
    42841 ttcggtatcc ggggtgacag ttgggaggct gcggaagcac atctggctga cactgcctct
    42901 acggttggca ccatgtctgc ggaacatgct tttgcagatg gtgagaccag aatccacctt
    42961 cccggcggca gtctgaccct gtcacagctg accgccatgt tgaatacgat tcctttggag
    43021 attaccttcg tggacatcga taatgtcaat cgttatttta acgaaggccc caaggtattc
    43081 aaacgtcccg gcatggcatt ggaccgtgag gtcttcacct gtcacccgcc taaaatcgaa
    43141 gaacaggttc gccgcattat cggagaattc cgtgcaggga atctggacca ggtaccggtc
    43201 tggatggaca agggcggccg gacattctta gtgacttatt acgctgtccg ggataagcag
    43261 gagcaatatc ttggaacact ggaactggta caggatatgg aattcgcaaa agagcacttc
    43321 cgctagggaa atgctctttt tgcttatatg ccgccagaag aaagcaaata tttctgtgga
    43381 tgtattgtaa ttttaagttg caatatgccg taattttttt acaccatatt actgcagaga
    43441 agctaaaatt tggttatact gtaagcatag gagattactc attagtgctt ttgtgaagga
    43501 tggtataaga tatgcttaaa aggttcacct tgaaaaatta taaaaacttt aaagatgaaa
    43561 ttacaataga ttttgaaaat attgcaggtt atcagtttaa tacagattgt ctttcggatg
    43621 gtgttattgg aaaaatgtta atttatgggt gcaatgcaac tggaaagacg aatctgggaa
    43681 aagcacttct gaatattact ttaacaatgt tcggtataat aagatataca ggtaatggaa
    43741 ttttacttaa tgcagattct aaagaggatg cggcaacgtt tcaatatgaa tttcagtttg
    43801 atgacacaga attaagttat aaatactgac tcatttccca aagaccagaa caatatagaa
    43861 gtatgatttt taaaagtttc ataattgctt ttagccctat ccagtacagc ttctttccac
    43921 tgaggcaatg atcccggaac attataggac ggttctattg ctcccatttt ttgccatgag
    43981 ccatgacttt ccagatttgt ttccgccatc atatagatcc ccatctcatc gcacatataa
    44041 taaaaaggaa tttgattagg ataatgactt gtccttaccg cattaatatg attcttttga
    44101 aaatattcca gatctaaaag catttctttt tccgtaatgc atctacctac atgtggttcc
    44161 catagtcgct atgtgcatcc attcggttta cttggtatat ttctggatca tctagccatc
    44221 tgatatctgc tttcatctta tacctcgctt tatatttgtc cattttctgc atttatcctt
    44281 tttgattttt aattatttca actatctgaa tcattctttc cagacatata tcaaaaaata
    44341 ttcgataact tttgcaaaaa taattttcat attctccatt catggcatta aaatccttat
    44401 ttcttcggca tcctccttta catattttat agaatttgca atttttacaa ttttctgcca
    44461 ctatttgaga ctgttccaca aatgcaattt cttttctttt cttatcaatt tcttccaaac
    44521 gattgcagtt aaagttacct agtttatatt cgtctaacgc ataaaaatca caagggtaca
    44581 cacttccatc tgcttccaca acattctgta taccacagct tcctctttgc tcacacgatt
    44641 ccggctgata gcctaaaaga atccctatat aattttcgaa ctgtcttata tacggttgtt
    44701 cacccttttt ccagtcctca taccataagt tgaacaattc cgtcaaaaac atcccgtatt
    44761 gttcaggatt taaagcatat ggtgtcttcc ctctgtcttc tcccaaagga tccagacatg
    44821 caatatactg ttgatatttc catccctgtt ccttataata attataaatt tccgctgcgt
    44881 gaccggccgt tttttgtgtt actactgtga gaatattgta atccacacca tattgatcca
    44941 taagttccgc tgccttctta atcctgtcaa atgtcggttc ccctcttctg ccatgacgat
    45001 aaaggtcatg tatctgcttc gtaccatcta ccgaaaggcc aatcaaaaaa tgattctccc
    45061 taaaaaattt gcaccattca tcatctatca agtacccatt cgtctgcatc gcattattta
    45121 cacgtattcc gcacttattg taatgtcttt gatattccat cgctttttcg taaaacgcaa
    45181 ttccacgtaa agtcggttca ccaccttgat acgcatagct tatcatgcct tccgcatgaa
    45241 acattgtttt acgaataaca ttcttaagtg tctgctctgt catgaatcca taggattccc
    45301 gcatcctttt tttcatttca tcgcaataaa agcaataatc gcatttcata ttgcacattc
    45361 ccgacgaggg ttttatcagt acactaattg gtggcatatt gttctcctgt ctttcgcctt
    45421 atacaagtga tacaaacact ttaattaccc cggccaatac cataacccag ataggattca
    45481 tctttgtttt tcgcagtata actacacata tggcaaaaat caataccatg acccattttg
    45541 ttccagtaaa agttatcgta ttgcttcccc agaaggccgt cataagaatc aaaattccgg
    45601 ctgacgcaat catcgccacg actgccggac gaagcgaacg caacactccc tgcagcatat
    45661 ccatactccg gtattttaag tacaattttg ccagcactgt cacaataatg caggacggca
    45721 aaatacatcc aagtgttgct actactgccc cgggtatacc tgcaatcttc agtcctacaa
    45781 aggttgcaga attaaccgca atcggtcccg gtgtcatctg tgatatcgta ataagatccg
    45841 taaactctga catagtcagc catccatggt cagtaactac ctgtccctga atcaggggca
    45901 tggcggcata tccaccacca aagctgaaca tccctatctg aataaagctc cagaataatt
    45961 gtaaatatat cattttccac ttctcctttt tgatagaagt atgcgtatta caccaattat
    46021 tccgcacgtt atcacaatgt acactacatt tatgctaaac acacatgccg caataaacgc
    46081 tcctaccata atgaccagag ataaagaatc cttttcctta ataataggca tccccatatc
    46141 caccgtaaca gaagctatca ctgcaccaac tcctgcctgc attccctcca acaactgact
    46201 gatcagccaa ttatttctga aagcattata aaatatagat attactgata aaatgacaaa
    46261 aggaggcaga acggtcccca gaattgccac aaagatccct gcagctcctg ctagtttata
    46321 acctacaaca atcgcaccat ttaccgctat ggctcccgga gaggattgtg ctattgccac
    46381 cagatcaagc atttcatttt cctctatcca atgatattcg tccacaaact ttttcttcat
    46441 cagtgtaaca atgacatatc ccccaccaaa tgtaaaagtg ctcaaatata gcgtagatat
    46501 aaacaatttc aatagtacta tttttttatt tttttcttcc atagactcct ttttagaaaa
    46561 atcgcttaaa tcttccgact taagcgattt ttctatatct ctacttattt tcttctatta
    46621 cttcattacc acgatccatc aaattcttga tggtgtcatc cagagactga gatccgagaa
    46681 gatacttatc tgtttcctct gtaagaatgc tatcaatttc actctgataa tcaggaatgt
    46741 atgttccata gctgtcaacc caatccggat catccataat cttggttaag gtatctgtgt
    46801 caagcttatc ctcaaatccg gcaataatct gctttacact atcagatttc acagctccct
    46861 tttcattaga aataaacatt cccttaatat ctacaccttc cgttgtccag aaccgtaaga
    46921 aatcatatgc ttccttggga tgctcggatg tctttgcaat agaaaagata tttccacctg
    46981 cttcattata atgttcacca tctttgtccc ataaaggctg gcgtgcaaat gtagttacga
    47041 aatcatgtgc atatttttca tttccaatgt cactaagcat agctgttccc atgggaagca
    47101 ttgcaatatt tccattaaag aactgatcgc gataattcat gttcagagct ttaacatctg
    47161 ccaacggtgt agaactatga tctacatttt ccatatcata acgcagctgc aagaacttag
    47221 ctaagttggg attatcaaaa gttaaggttt tatcatcatt aaagattgtg cttccaccct
    47281 ttccactact gattccccat aagttacagc tcccccagga gtgaaaatag gaaccatatt
    47341 ttgtgtccgc tccacttcca cttgtcagct tttcagcata ttcacgataa tcatcccagg
    47401 tccaatccaa agaaggtaca tccagtccgg ctgcatccaa catatctttg ttgataagaa
    47461 ccagattata tttcatttct cccggaatac catatgtctg atcgtttact cttacaatta
    47521 cattgtatgc gtcctctgca gtagttcctt cctcagtaaa ataatcatcg agagacaggt
    47581 aggatccaga ctccgcacgc acaatatagg aagcgtaatt aggcgccatc agaatatcca
    47641 ttgcttcgcc acccatcagc ataatatcag tctttgtcag atagtcatta ctgttcatgt
    47701 cacctacata atcaaactgc acattaatat taggatggag ttccttgtat gctgccgcaa
    47761 ccttttggtt ctgttcatca tgattggtct gccagctgac aaaacgcaga gtaatttcct
    47821 cctgattttc atctgctgcc tgagtagttc ctgttgtttc cttgttctca gtcgtggtag
    47881 ctgtattcgt tgtttctgtg ttttctccac cggaacatcc tactaaggcg gttgccataa
    47941 tggaagccag taccaggctg agtcctctct tcatctttct cataataatt cttctccttt
    48001 catgtgtgta tatgtaaatg ttacaaatac ccttacatca accctttacc gcacccgaag
    48061 caattccatc aataatataa cgttgcccga acaggaacag aataattaat ggtatcgttg
    48121 ctgatacagc tgcagccata gtcagggaat agatgcttcc agattcagaa gcaaataatt
    48181 tcatacctag ctgaattgta aatagtttac ggttgcttaa aaaaatcaat ggtgtttgat
    48241 aatcattcca cgtccatacg aatttcaata ccgctaatgt agcaatagag ggctttacca
    48301 acgggaatat aatttgaaaa aatattctca agtgaccagc accatctatt ttggcagatt
    48361 cacagagcga atccggaatt gccatcatgg cctgacgtag caaaaatact ccatataggc
    48421 taaacatggt catcatgata attcccatgt gtgtattata aaggttcatt ttctgcataa
    48481 ttacaaatcg actgacaatc ataacctgtg ccgggatcat cattgttgcc aaatacacta
    48541 aaaaaagctg atctctgcct ttccacttaa tctttgtaaa tccgtaagct cctaaagctg
    48601 ataccaaaat ttgaagaacc gtggatatta ccgtcacctt aatgctattc aggtagtaca
    48661 ttccaaaatt ataggcagat ccccacactt ctttatagtt ggcaagtccg acactgtgtt
    48721 ccggaatcca tttgatagga aactgaaata cttcgttttc cggtttaaat gatgtggaaa
    48781 tcatccatag taacggagtc agcatagtga agccaatcgc aaataaaaaa actgtaaaaa
    48841 tcactttgtg tatctgtatc tttttcatat actgcctcct tcctattcat tagcaaaacg
    48901 atcttggaat ttccactgta ctaatgtaac tgcgaaaatc aaaaggaaca agacccatgc
    48961 cagtgcacag gcgtagccca ttttaaaatc tgtaaatgct gtcttatata tgtaataagc
    49021 cattactgaa gtagaatttc ccggtccacc tgctgttaat acgctgatca tatcgaatac
    49081 cttaaatgaa ccgataattc ccattatagc caggaaaaaa gtcgtcggac ttacctgcgg
    49141 tactgtaata taccggaatt gctgaatgct ggatgcgcca tcaatctgtg ctgcctcata
    49201 caaatcagca ggaacatttt taagtcctga catatatacc gcaacataat atcctacctg
    49261 cgaccagata ttaatcaaca taattgcaat caatgcccaa tgttcatcaa ccaaccattt
    49321 cggagggttt tcaattccaa tagtacgcag gaactcattg atcgggccgt aactcggttg
    49381 aagcattacc tgccatactg tacatactgc tactacactg gcaatgtatg gaataaatat
    49441 cattacccgt atagcagacc ctccatatgt atgtctgtta atcagatctg ccatcactaa
    49501 tcccaaaatt aacagacaag gtactgtcac cgcagtaaat aaaatgttat ttcttaagga
    49561 tacaacaaac caatcatctt taaacagttt caaataattt ctcaatcctg taaattttat
    49621 tgaatcccac cccttcagaa agttccattc cgtaaaactg attcccaatg acatgatcac
    49681 gggtatcaat ataaatatga tgaatccaat cagccctgga agaacgaaac agattcctgt
    49741 caacgtttct tttcgtttca tacaatgcct cctctttgta attgtcaaaa tgacgaattc
    49801 acgtatcatt tttgtgtttt ttactctaaa ctgcacaatt aaaaaagtaa agttgacatt
    49861 tttccttggc aatgaaatta gtacacttcc atacgaattt cactttatgc cattgacatt
    49921 cctttcaaac atcctatatt atgtatacac attacaaaaa attgaggtga ttatatggaa
    49981 aaaatataca attttgccaa aaaagatttt tttatttttg catctaccta tgttgcaatt
    50041 atttttttgg ttcttttatg tttttttcct gtatgccggg catttgaacg ttctgaacaa
    50101 aatcaggcta ttgcggaaat acgtgattac gcctccagta tgttaggaga actggatctc
    50161 caagagcagg ctatttttaa cgccaccaga aatctataca gtgatcgcga ttttacgtcc
    50221 atttattata acagtacgcg cagttcatct tcttccttat tctatgacat gacactgttg
    50281 caaaaacgaa ttaaactgta ctatcaaaat cttgagtatg ttcaggatgt attggtatac
    50341 cttcccaaat tcaattatgt acttacacaa aattatattt ttgacagccg agattctttt
    50401 tattcctata taaaatcaag tgccttcgaa ggaactcctg actggttaga aacctttccg
    50461 ttaaataata ctggcatttc tttttatagt gaccaactaa cagattgcgt taattccgaa
    50521 gaaataagaa actccttgaa cttttcctat tattttccta cttacggaga tcccaatgta
    50581 agaatgctgg tgattgttca gttagacccg gatgctgttg ctaaaagttt tctcttaaag
    50641 tctgcttctg actatggatt tactgtattg aaaaatggcg atggtgaggt tctagttcaa
    50701 cataactatc cagataattt agcagaaaaa tcgtgggaaa taataaattt accgcaaagc
    50761 gatcaaggtt atcttacaat cggagtcaaa aatgaatact tcaaaccaat tcatcaggct
    50821 acgatccgtt taatttttgc tgatctgggg atagcatttc ttttaggcac gattgcatcc
    50881 atttactttg cttacagacg ctcgcaccct attgaacgaa tccttcatat cattcacgat
    50941 atggatcgac agcccaatgt gcagaattct tttttggaaa ttgaaggcac tgtattaaat
    51001 atgatctcgg aaatcagtca ttgcaagaca accatcgaaa gtcttgatac tatggttgcc
    51061 gacagcttaa aagaaaaact ttttatttct ggtctaagta cagagcagga gattcactct
    51121 tttcaacagt attttgggga tttttctttt tctatgaatg cccttgtttt ttctataccg
    51181 gaaaaaacga ctttttctaa agaaatatca ttaaaagaat tgctttatga gcagctttta
    51241 cagctaagtc agaaaaccat tatattatat caggcagaaa actatatata ctgcctaatg
    51301 gaatctgttt caggtctgtc agatctgttg agcaattgtt taaaacatat acgtgaactc
    51361 cataatcagt ctgttaaagt gggcatcagc aatccttttc aggatatttc ctacacccag
    51421 catgcttcca ctcaggcaaa acgacgttta tccgccggat accgcattga cggtgtatat
    51481 gtatttactc acacttattc ctctcgtgct gtccgctctt tgattagcat tcaggatctt
    51541 gacaaccttc aacgcgcttt gcttagtgga aatggtcaaa atgctgatcg gattttggaa
    51601 accatccacc agacaatcag tcactccgaa caaaattctg tagaactgcg ccagatgttc
    51661 ttttctcttc gttccgttta tgcaactgtc tgcaatcagt tttcattgga agcagaacga
    51721 aacggggaaa cacgctacca cgctcctatt cttccaaatg atttggacga atacgatctg
    51781 gattccgttt atgaagtatt ccgtagtctg aatattgaat tgcaacagca atatgagatt
    51841 gtaatggctc gtaccgccag aagcctcggg acagatattt tggcttacat agatcagaat
    51901 tataccgatc ctaacctttg tgcaggagtc attgcagatg ttttccatat ctctgaaaaa
    51961 tatgtatttc agttactaaa aagttgttgc aacgaaactt taaacgacag aatactacgc
    52021 cttagaattg atgaggggat ccggcttttg acaactaccg atttgacgat tactacgatt
    52081 gcaaagaaaa cagggttttc atccagcaac acaatgtaca aagcgtttat gagagtaaaa
    52141 ggaatttccc cttcttccta tcgtggtaaa gaaatataaa acaggaggct atatatttat
    52201 gaaagcaatt atgataatgt ttgattcctt aaaccggcat tttcttccca actatggctg
    52261 cagctggacc gtcatgccac aatttcagcg cctggctgaa aaggctctta cttttgactg
    52321 tttctatggc ggaagcatgc cctgtatgcc cgcacgcaga gaattgcaca cagggcgcta
    52381 caattttctt cactcatcct ggtgccctat gcaaccgttt gacgactctg tcattaagcg
    52441 tatgaaagat gcgggaattt acactcatat ttccacggac cattttcatt actggcagga
    52501 tggcggctct tgctatttaa ccaaatttga cagtcatgaa attgttcgtg gacaacaggg
    52561 agatccatgg atgggacagg ttgcctggcc tgactaccct gatacgcttt ctcgacgcaa
    52621 aaatacacaa agttggcgtc acgactgggt aaaccggcaa tttatcacaa ctgaaactgc
    52681 tatgccacaa tgtaaaacct ttacaagcgg ccttgatttt attgaacgaa atcataatga
    52741 agacctgtgg ttcttacaga tagaggcttt tgaccctcat gagccttttt atacccaaaa
    52801 tgaatataaa aaattatatc ctgataacta ccatggtaaa aatctggatt ggcccgatta
    52861 tggaaagaac gaatatggag aggccgctac cagacatgtc cgctatgaat acgcctcact
    52921 tatgagtatg tgtgaccact atcttggaaa agtattagat atgatggata aatacgacct
    52981 ttggaaagat accatgctta ttgtcaatac tgaccatggc tttatgcttg gtgaaaaaga
    53041 atggatgggt aaaaatgttc agcctatgta tgaagaactg atccacatcc cgttcttcat
    53101 ctatgaccca cgaaatccca ttcaaggtga acggaggaat cagcttgccc aaactattga
    53161 tattgtacct acattagccg aattcttcca aattgctccc ccagagtata tggatggtca
    53221 tagtttgacc cctgtcataa gaaatgatac acccatacgt aattatgcac ttttcggtat
    53281 ctttggtggt catatctgta ttacagacgg gcgatatgtc tatatgcgtt cctgtaagga
    53341 tccagaaaat cagccattat atgaatatac cgctatgcct tgccatatgg atcggccttt
    53401 ttcccgggaa gaaatgtcag aagcagaact ttgtgataag aatgctttca attttatgaa
    53461 aaaaagtggg gtttttaaag ttcctgttca ccactctaca gattcttatc gttttggtac
    53521 tatgttgttt gacctcctta cagatcctga gcaaaaatgc cctattcatg acagcaatat
    53581 agaagatcac ctctgccaag caatgaagaa aatgatgaag gataatcagg cacctaaaga
    53641 acaatttgaa cgaatcggtt taaccgaata ggcaaccaca ataatttctc acattgcaaa
    53701 atgccagaaa cagattttta ccattctgtt tctggcatta cttaatgtgc aattataaca
    53761 tttttctcca tcgttcacgt attgcaaacg ctgctgtctt aggctgtcgt tccctcgtaa
    53821 atataccctt tttattacca tcaaccctct ttagtccaaa tgccgtcata aaatctgcaa
    53881 atgcccaaat atgttcccct atcacaaaat cgcattcatc catggcttta ttgttttctt
    53941 caatacacct cttttgatat tcctctgaaa aaatcacttc ggggagttta tgcaatcctg
    54001 ctattgtatc tgcaccatat tctgtcacaa tgacaggttt tccgtatttt tcatgccact
    54061 ttatcaaatc tgtctttagg ctaggatata tccgttctat tttgccagct ccgacatacc
    54121 agccaaaata tctgttaatg catactacat caaaaaactg tgatatctta tcctcctcca
    54181 catcaacatt catggttcct gttattggtc tttcactatc cagttttcta atatattttg
    54241 taacttcctc aaaataattt cttgaggcct cttcgctgga atgcggttcg tttgtaatac
    54301 tccacataac aacacaagga tggttcttat ccctttggta taattccttc aatacctgct
    54361 tatgatatgc tagtgttttt tcatttactt tatcttctgt gaaaatacct ccattttcgc
    54421 catcaaaaaa gttcattcca accgccggta cttcatcaat aaccacaatt cctttttgat
    54481 cggccatttg catcatttct tctgcgtaag gataatgtga agttctaaag gaatttgcac
    54541 cgatccattt cagcaattca cagtctctga catttaatgc ctcatccaat cccttgcctc
    54601 tgatatcact gtcttcatgt ttaccaaatc ctttaaagta aaaaggcttt ccattgatta
    54661 agaattgttt ttctgtcagc tgaatcgtcc taattccaaa tggcaaagta taatgatcct
    54721 gtccaaaata aatatccagt ttatataaat aagctgatcc cggctgccag agttgtgcgt
    54781 cctttaccaa aaccatatct ttcatctcat tactctcagc aactacctga ttcttttcat
    54841 cccggatata tacttttatg aatttctctt ctgcattagt ttttatttca taatgtacca
    54901 tcccatcctt atcatctact gtcgttctaa ccgaaatgtc ctcaatatat tctttgggcg
    54961 tacaatataa tctgaccggt ctatgaatac ccgaataatt aaagaaatca aaatcaaact
    55021 tttgttccat ctggccttcg ggatgcatct catccctaac atattctata tgaccaacag
    55081 gaaggcaatc ccattccagt atatttccta ctgctactgt caaacgattt tccccttcat
    55141 tggccatttc cgtcacatct gcctcaaagg gcaaaaaacc acccttatgg cgagtaattt
    55201 cttttccgtt cagatatatt acagcatgat gcgtcacact tccaaatcgt agtacaagcc
    55261 tctgatcttt ccataatctt ggaactgcaa attttctctc ataccatacc caccctacat
    55321 gatctctcag ttcttgatct gtagtaatat cattatagct tgatggtaca ggcatatcga
    55381 ttacatcttc caatggtttt tcaaaccatt tttcttcaaa tccctgttta taatagttat
    55441 ctctcttaaa tttccagata ccgtttaact ctttaatctg tcttgcttca ttatctaccg
    55501 gaaataacat atcaaatctc cttttccctg gcatcatctg ttcttatcca catagtaact
    55561 tttcatcttc aaaacgtttt gatatctata ttgtacctca actgtaaaag aaatctttgc
    55621 attttctatt gtgtttatgt catatcatgc tatactaatg taaatcaatt taggagtgga
    55681 tattatacgg tatttaccat gtcaaaagag aggatcagct tgtcaacaga ttttcaggaa
    55741 aacaattata caatagagga attaaatctg cccatcagta tctcccatgt catttcaaaa
    55801 ggagaagact gctctgactt acacattcac tcagatcagt atgaaatata tgtatttctt
    55861 agaggagata ttgattactt tgcgggtaac atcagaaagc atctggttcg cggagatgct
    55921 ctgatagtat ctcctaacga actccatcgt ccatatctta taaaagaaac ggcgtatgag
    55981 cgtgtcatta tccatgtaaa ccaagatgta ataaagaaat tgtctactcc tgtcagcaat
    56041 atatacagat ttttagaaca gtataactgc tgtttcttcc atctgcccga tgaaatttta
    56101 aatcaatttc taaataatga aaggatgatt caacaatatt ataataccaa aaatgagttt
    56161 ggatccgata ttcttttgga aacatggtta agtatcacgc tgatacattt aatacaattt
    56221 ctccaaaaaa atcagaataa agtaaatgaa gggattactg tttttcctga tcttgttaaa
    56281 aatgctatcc aatatgtaga tgatcattac acggaaaata tatcagtagc ttcaatatcc
    56341 agatacctga atataagtag ttcccatctc aatcaccttt ttaaatatta cactgggaat
    56401 actctgtgga actatgtaat atccaaacgt atgctcatgg ctcacaaaat gattcttgag
    56461 caaaaatcaa ttaccgaaat ctgttatgcg gttggcttca agaactactc tcattttatt
    56521 aaaaccttta caaaaacctt tcatgtttct ccaaaaaaat atgaaaaaga gattctaacg
    56581 gatcgcattg tgtaactttc ctattaaaaa actttacacc actattttct gtcatattaa
    56641 agatataaaa aacaggaggt tactcaatga aacaattatt ttacaaagaa catcttattg
    56701 aaaatgaatc tgttcgtatt gctgaaaact tagaaaaatt tttattaggt atcggcctag
    56761 aacaaaatga cattgatcat gctaaaaacc ctattatccg aaatttatta atgaaaatag
    56821 tacatattaa caatccacgt atgcatcaat attttgtaga tgatctactt actacttcag
    56881 atccagaaat caaattatat aaattcaaca gctaatatca actagcaaag attttcgtac
    56941 actctcataa gatataattt tgaatatagt ttgctaaata ttacattatt tgaagagcat
    57001 ttccgttaag gaaatgctct ttttatctct tcctgcgctt cttccacata aagctgcgca
    57061 ttctccagat tccgccggat ctcttctttg gtaacctccc gttttacttt cccaggattc
    57121 ccaataacca gtgaattatc cggaatctcc acattctgtg tcaccagcgc tccggcaccg
    57181 atgatacagt tcttcccgat cttacagccg ttcatcaaaa tagcacccat gccgatcatg
    57241 gtattgtcgc cgatcttaca gccatggacg atggctccat gtcctatggt cacatggtct
    57301 ccgatctcca ccggatatcc gctgcccaga tgcacgaccg cattgtcctg aatattactc
    57361 tcttttccga tgacgataga atctctgtcg ccccggacca ccgcattgta ccaaatcccg
    57421 ctgttttctc cgatcgtcac atctcccagc accactgcac cgggtgctat aaatactgtc
    57481 tcatggatct gcataaacgt tttctccttg tgttctcatc tatttattat aaagtacaca
    57541 ctgcatataa cggtatattt ttcatccagc cctgatcaat atatttcttt gccgagaaac
    57601 gaattgccat ttgcggatga tatttttcgc aatagcattt cagactctgc gatttcaggt
    57661 ttgtctctgc ttttacttct atgggaacaa catcttttcc catttgtatc ataaaatcct
    57721 gttcaaaagt cgccttttcc gttccataat aataaggcgt atattttgta tccgagacaa
    57781 tctgctgcaa tacatactgc tcagtcagcg cacctttaaa ctcgacaaaa atatcattac
    57841 cttccaggat actcatggca tccaagtcgc tcattgctcc cagcaatccc acatccagca
    57901 taaacagttt gtacacgttg atctccttgt atgcagccag cggcacatgt ggctcattca
    57961 cacgatttac cttatgaatc agtccgcaat ccaagagcca ctgtattgct atctcaaaat
    58021 ccgcacttct tgcacccttt ttaatctgtc caaagaaaaa cttcttattt tccttcgcca
    58081 actgtatcgg aatagattcc cataccatac gaattctggg aagatctttg gcatctatat
    58141 gttttccaaa gtctccccgg tactgacgta ctatgtcttt ttgtaactcc cgaacaccgg
    58201 catagtcctt ctgctctctg aaaaggtcaa ctactgcagg cattccaccg gtataataat
    58261 aattttttag ccaaaatagg tatttatccg aaaagttgtc tataatggaa aagtccttgc
    58321 tgtccaatgc cccagacaaa gcttcttccc ccatggcgca tagaaattcc ctaaaattca
    58381 aaggatagag gtccaacagt tccaccttgc ctaccggata agatactccc tgatgaatcg
    58441 ctactcctaa cagggacccc gctgcaagaa catgatactc cggtgcatct tcgcagaagt
    58501 acttcagaga ttccaggact ctcggtgcat cctgaatttc atccagaatg atcaacgtat
    58561 ctccgggagt aattgtgacc cctgtttcga tattaagatt cattagaatt ctctgaatgt
    58621 caaaatccat ctcaaagaca tcgctcattc tcttattatt ataaaaggat acatatgcca
    58681 ctttggggaa aaattgtctt ccgaattcct tcattaacca tgtttttccc acctgtcttg
    58741 cgcccataat aatcatgggc tttctttttc ccttttcttt ccagctttgt aattgtttca
    58801 tggcaaatcg tttcattggc ggcctccttt cttacataat atagtttacc acattttttc
    58861 cgatcaaatc aattgtatat tcaacatttt ttctgaccaa tattttatga tttaatacat
    58921 tttttccaat caaaaaggaa cattggtaaa tagcaaattt aacttccgtt ttgcaaaggt
    58981 aacttcttct tgaatta
//
LOCUS       DAWDHD010000002        52543 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_60_length_52543_cov_11.569349, whole genome shotgun sequence.
ACCESSION   DAWDHD010000002 DAWDHD010000000
VERSION     DAWDHD010000002.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 52543)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 52543)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..52543
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60_length_52543_cov_11.569349"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    44552..44651
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT        12918 a        14044 c        10366 g        15115 t
ORIGIN      
        1 ccgccgaaag cactcatatc catgccgggc atggagtaca tcttcatcat atcctgcata
       61 cgtctgctct cttcggacag cgtgatcatg gaagagatct tcttgttctt cagcttctct
      121 accttgacag tgatcttgtc gttcttcaga gctttcttca tcagcttgct gatgctctct
      181 gcctgctctt ccatctcttt ttcagccttc ttggaagtct tggacttcag ggcatcggtc
      241 acatccgcat cgatacgctg gaacttgatt ccctcattct tctgctccaa ctgggagatg
      301 aagggctgat cgatattgtg ggtcaggatc acagcatcca tcttggcagc cttgaacata
      361 ttaatatact ggctctgctg ctgttcatcc gttacatagt agatgatctt ctccttcttc
      421 tcttcctcgg tattctcccc ggaagcctca tcttcagatt tatcctctac gacctcagct
      481 tctaccttgt tgccgttctc atctacagca ttttccttat tctcttcctc gtcctgaggc
      541 ttgatctcca gacactcggg cagagtcaga tatttgccgt ccagattctt gaagaggata
      601 taatcattca tcttctcaca gaacttgtca tccttcaggc agccgaactt gatgaaggga
      661 ctgatgtcat cccagtattt gtcgtactct tccttatccg tcttgcacat accggccagc
      721 ttgtccgcta ccttcttggt aatgtactcg gcgatcttct tgacaaatcc atcattctgc
      781 aatgcacttc tggatacgtt cagaggcaga tccgggcagt cgatgacacc ctttaacagc
      841 agcaggaact caggaatgac ttccttgatg ttatctgcga taaatacctg attgttgtac
      901 agcttgatgg taccttcgat ggactcatac tccatgttga tcttcgggaa gtacaggata
      961 cctttcagat taaaaggata atccatattc aggtggatcc agaacagagg ctccttgtaa
     1021 tcctgaaata ccttgcggta gaagtccaga tactcttcct tcgtgcactc attgggatgc
     1081 ttggtccaca gaggctgtgt ctcgttgatc aattcaggac gctttctgat cttcagcttc
     1141 ttaggctctt ctgttccctc agcagcttca gcgttttcac cttctgccgc ttccttcttc
     1201 tccggctcga tctcttccag gacttcatca tcgggaagct tttcctcttc cttgatgatc
     1261 tgggtctcct tggtgttcgc cttggacaga tagatctcgg taggcatgaa ggaacaatac
     1321 ttcttcacga cctcacgagc cttgtactca ttacagaact ccagacaatc ttcatttaaa
     1381 aacagggtaa tggtcgtacc gacatccgtc ttgtctccgt cttccatgga gaactcggtg
     1441 ccgccgtcac actcccagtg tactgccttg gcgccatcct tgtaagataa cgtatcaata
     1501 tgtacttcat ccgctaccat gaatgcggaa tagaagccca gtccgaaatg accgatgatc
     1561 tggtcggaat tgctcttgtc cttatatttc tcaatgaaat ccgtagcacc ggaaaatgcg
     1621 atctggttga tatactcttc cacttcgtcg gcggtcatgc ccagaccgtt atccttgaag
     1681 gtcagtgtct tcttctcagg atccacgatg acatccacac gtcccttata accttccgga
     1741 agagaatatt ctcccatcat gtccagtttt ttcagcttgg tcacggcatc gcagccgttg
     1801 gagatcaact ctctgtagaa aatatcgtga tcggaataca accacttttt aatgatcgga
     1861 aatatgtttt cactgttaat tgataaactg ccgttttttg ctgccattat taccacatcc
     1921 ttttctaaaa ttgcacagcc atggctgact gtttcctaac tgattctaag tgtaggactg
     1981 taaaattgaa aagtcaagac aaatttagca ctcattataa atgagtgcta acaatcagtc
     2041 aaaaatccag tatttatcaa tatttccgta attctaaaat tttaataaag cgtgtaaaaa
     2101 accagaaatt actcctccgg aaagtacttt gcatacacat caaaaaagtt gtccgtagtc
     2161 acctgttcgt ccatgatcag cgtgatctcg tcccacagct tgtcattgag ctgacgcacc
     2221 agtgtattca caaagctgtc atactcctgc ccgaagactt cccgtttccg ctcttctaca
     2281 atctttaatt tattagcgtc cgtctcttcc cgatccagcg tattgatgca tttgataatg
     2341 taatagcctc gttcacattt cacaacctga ctcacctcgt ctgttgccaa attgaaagcc
     2401 gcttcctcat aagcgctctc cgcttctcct ttgccaaagg aataggtgat ctcggaatcg
     2461 tcgctgtatt tggatgccag ttccgtgaaa tcgtgttctc cgtccaccgc caggtctctt
     2521 acctcgcagg ctttctcata ggcactctga atggagcttt ccgaataagg cttcaggttt
     2581 ccgtccatat ccgtggatgc tgttgcaaaa aagatctgct gcaccgtaat tttgcgtgcc
     2641 tcgtcatcac tgatctccgg attgatgtcc tgtataatca actgatagac cttgtctgcc
     2701 atggcatatt cccgatacag attctccaca atctcctccg tggcacccat ggcctcgatc
     2761 tccgtatcat ttaaggaaga ataatactcc tgtgccgcct ggacgacctt tgcctcctcg
     2821 gcttcatcca acgtgacctc tttctctttt gccagcaggt acatggtctt gatctgagca
     2881 attcttgcca gcactgtctc cttgacatta tcctccagag tcactccgtt cagggaagta
     2941 ttccagatct ccgtcccgta gacattctca tattgattct gggtcgtggt caggtagatc
     3001 atgatctccg ccttagagca ggactcgtcc ccgatccgaa atacctcatt tttcccaaat
     3061 cctgtggtaa ataccacttt tgtgccgtca ctgctgccgc aggcggtcag tcccgtcacc
     3121 atacttattc ccagcactcc cgccaccaat accgcggtga tatgacatct atttttaaaa
     3181 aacataacat ttcctacttt ctcaaagtct ctaaattctg attataccct atttttttct
     3241 tctgtgccac ggtttcttgg aagttcagaa aaatctaaga attctacaga caaaaaaatg
     3301 cgtgcggata ttctccacac gcatttttaa tattattcaa ttttgatacc agattactca
     3361 attacatgga tccagccctt aggagcttcg atcctgccca tctggatacc ggtcagagtc
     3421 tcatacagtt tcttggtgac aggtcccatc tcggtcatgc cgctgggcag gcagatttct
     3481 ttgccgtggt caacgatctt gcctacaggg gagatcactg ctgcggtacc acacagacca
     3541 gcctctgcga agtcttttac ttcatccagg tatacttctc tctcttctac ttccagtccc
     3601 agatattcct tggcaacata taccagagag cgacgggtga tggaaggaag gatactgtcg
     3661 gacttgggag ttacaacctt gttatccttg gtaacgaaca ggaagttcgc accgccggtc
     3721 tcctctacct tggtacgggt agcagcatcc agatacatat tctcatcgta gccttccttg
     3781 tgagcggata cgattgcatg taagctcata gcatagttca ggccagcctt gatgtgaccg
     3841 gttccgtggg gtgccgcacg gtcgaagtcg gtgacacgga tggtcagagg cttaacgcct
     3901 cccttgaagt aaggtcccac aggggtgcac aggatacgga actgatactc atccgcaggc
     3961 tttacaccga tgacagggtt gctgccgaac atataaggac gaatgtacag agttgcgccg
     4021 ctgccgtaag gaggcacgaa agcatcgttc gccttaaccg tctcagtaac agcttccacg
     4081 aaacgctcct tggggaaaat aggcatctcc agtcttgctg cggaagactc cagacgctca
     4141 ccgtttaagt caggacggaa ggttacgata tgtccgtctt ctgtggtgta ggccttcagg
     4201 ccctcgaata cggtctgtgc atactgcagt acaccagcac actcatttaa tacgatgttg
     4261 gggtcctgtg tcagctgacc gtcatcccat ttgccgtcct tgtaattgga cacatatctg
     4321 taatccgtag ggatataacc gaaaccgata ttaccccagt caatgttctt cttttccatt
     4381 ttgtattcct tctttcctcg gtttaccact ctgcctgttg atttctctta acagaattta
     4441 ttgattttca ttattatacc atttgtaata aaagtcaata tacacattta tgctttccag
     4501 tgcttttttc agcgaaaatg tgtcgttttt cttcttttca cagttttttc agattaaaat
     4561 cactacatat ctgtcattgc atttaagact ttttccgctc atatttcaag aactggtaag
     4621 tgatgtcaaa atagacctgc tcatcgctgt ccgcggtgat ctcccactcg ggatcactgt
     4681 ccagatccgg gaaataggca tccgcctcgt aggcatgatc gatcttcgtc acatgggcca
     4741 cgtcacagta aggcagcatc tggcggtaaa tactctctcc gccgatgata tagatattct
     4801 cttcgtcata tttcttcagt tcttccagca attcttcaat agagtgtacc acgatcgcat
     4861 ccttgacctg ataattcgga tctttggaca aaataatgtt gatgcggttc tttaaaggca
     4921 tgccctgtgg gaaagtctcc aaagtcttgc gtcccagaac cactacctta cctgtcgtct
     4981 cttcgcggaa atgcttgtga tcgttgggga tgcggatcag cagttcgttc ttgctgccga
     5041 tggcccagtt gttgtctact gctacaatta aattcatggt gtacctcgtt tctgtttgtt
     5101 tctatgataa taaccggttt ctccgtgctt cgcactcacg aaaccgtact gatattcgta
     5161 ttccgggcta ccgccctact tactcatgtc agattgcaat cggaatatcc ctgatctgct
     5221 cccctgtctg gtaaccctcc agcttcacgt catccggtgt aaactggtaa aaatccttaa
     5281 tctccgggtt cagccagaac ttcggtgccg ggaaaggctc tctgcttatc agttccttaa
     5341 tggcatccac atgtcggtca tagatatggg catccgcaat gacatgcacc agctcaccca
     5401 cgttcatatc gcagacctgt gccagcatat gcaccaacac cgcatactgt accacattcc
     5461 agttgttggc tgccagcacg tcctgggaac gctgattcag aatggcattc agggtcagcc
     5521 tgtcctctcc ctttttctga gtcacattaa aggtcatgct gtaggcacag ggatacagat
     5581 tcatctcatg caagtcctga tgcacgtaaa tattggtcat gatgcggcgg ctgaagggat
     5641 tgttcttcag atcgtagatc acacgatcca cctgatccat catgccctct ttatactgat
     5701 gtttcacacc catctggtaa ccgtaggcct taccgatgga accatccgca tccgcccatt
     5761 catcccagac atggcttttt aagtcatgaa tgttgttgga cttgcgctgc cagatccaca
     5821 acatctcatc cgtggcggat ttcagcgccg tctttcgtaa ggtgaggatt gggaactctt
     5881 tcgacaggtc ataccggttc accactccga atttcttaat agtgtaggca ggggtgccgt
     5941 ccggccaatg gggacgcact ttctcgccct ccgtgctggt accgttggtc agaatgtcat
     6001 tacacatatc gataaaaata tgatccgcta aactcatggt gtctccttat tctatcttct
     6061 aatatataac cgatttctcc gtgcttcgca ctctcgaaat cgctctgata ttcgtattcc
     6121 aggctaccgc cttgcttact catatcagtt tgattctctc atcagccaaa agccttgact
     6181 gcaagcagtc acgcagaagc tgatggagaa tcttattatg ttatataacc gatttctccg
     6241 cgcttcgcac tctcgaaatc gtacacccct tcgtattccg gactctcgtc ctgcatactc
     6301 agggttgctt atgcctctca taggaagtat ttcctgcctg caagcagtca gaaatacttc
     6361 ctatgaatgc acttatattt cccatttgtc acacagggaa ttgatctggt cggcgaactt
     6421 ggccatgtcc ttattggtca tgacaccctc gctcttggta accagcgcca acagacgtct
     6481 tgcagaagcc aacagcctct cgaagactcc ggtgacaggc ttcgtctttt tcttcacggc
     6541 aaccggtgca gcctcatagg taaatttatt gctgatcagg tcgaactccg tgccactata
     6601 aggtgcgtaa gccctctgcc catactcgat cttcaggcat tcggtgaatg cagtgcaggc
     6661 actgtcttca ccgtgtacca caaacacctt tttcggcttc tcttcaaagg cctggatcca
     6721 ctcgatcagt ccttctttgt ccgcatggcc gcttaatccc gccagtttcc ggatctgtgc
     6781 ccgcacctga atggtctcac cgaaaagctt tgcctcatcg gcaccctcta ccagcgctct
     6841 gcccagggtt cccacggact ggtaacccac gaagagaatc gtacattctt ctctccacag
     6901 gttatgcttc agatgatggc ggatacgtcc cgcatcacac ataccggaag cggaaatgat
     6961 gaccttgggg gtctcgtcaa aattgatggc cttggattcc tcactggtga tggacagctt
     7021 caatcccgaa aatgcaatgg ggttgatgcc ctctttcaca tattccatag cctcgtcatc
     7081 aaagcactca aaacgattct cctgaaatat accggtggcc tccaccgcca tgggactgtc
     7141 cacatacacc gggaaatcct catgtccttt gaccagacgt cccaccttga ttttccgcag
     7201 gaaatacagg atctcctgag tacggcccac agcaaaggaa ggaatcacca cattaccgcc
     7261 acgatccagt gtctcctgca gtacctttgt caattccgcc acatagtcta gtttgtcctt
     7321 gggatgcatc ctgtcaccgt aggtagactc catgaccaca taatccgctt cttttgtggg
     7381 tgtgggatcc ttcaacaacg gctgatgctt gttgccgata tcgccggaaa atacgatctt
     7441 cttcgtattg ccgtcctctg tcagccagac ttcgatgctg gcagacccca gcagatgtcc
     7501 gatatccgtg aaacggatct tcacgccctc gcacacttct acgatcgtat tgtaatgaca
     7561 gggaaccagt ctgcggatca cgccgtccgc atcctccatg gtatacaacg gttccaccac
     7621 tgcattgttg gcagaacgtt ttgccttgcg gttcttccac tctgcctcag cactctggat
     7681 atgcgcacag tcacgcagca tgatgctgca cagatccgcc gtggcatccg tggcatagat
     7741 ctggcctctg aatcctctgg catatagaag tggcagaagt cccgaatgat ccacatgggc
     7801 atgcgtcaga aagacataat cgatcatgga ttccgccacc ggcagcggaa cattctcaaa
     7861 tacattgatg ccctgctcca taccataatc caccagaata tgctttccgg ctacctcaag
     7921 ataatggcag cttcctgtta cttcatggtc tgcccccaca aacacaagtt tcatagatct
     7981 acctcccgtt ttgttagttt attttaaagc attggctgtt tcgtagaact gataatagat
     8041 ccctttttgt tccatgagct ggtcgtgatt gccttcctcc acaatgccgt catccgtcag
     8101 caccaggatc ctgtcggaat tgcggatact ggaaagtctg tgggcgatag tcagagtagt
     8161 acgcccctcc gacaattctc ccagagattt tgccacggcg aactcactct cattgtccag
     8221 tgcggaagtg gcctcatcca gaatgatgat gggcggattt ttcagaaata ctctggcaat
     8281 actgatccgc tgtttctgtc cgccggacag ctttacaccc cgttctccca cataagtatc
     8341 ataaccgtcc ttcagtccca tgatgaattc atgagccccg gcacgctttg ccgcattgat
     8401 cacatcttct ttcgaagccc cgggacgtcc ataagcgatg ttttcataga tcgtaccgga
     8461 gaaaagatac acatcctgct gtacgatacc gatattgccc cgcagactct ttaacgtaaa
     8521 atgcttaatg tcctggccgt ccaacaggat cctgccttct gagacatcat aaaatctcgg
     8581 gatcagattg cacagcgttg tcttaccacc accggaagga ccgacgatgg ccaccttttc
     8641 tcccgcgtgg atctgcaggt tcagatcagt gaataccttg ttatggtcat ccggatattc
     8701 aaagcttact ttttcaaaag agatttctcc cttcgggtcc ttcagttcta tggcatctgg
     8761 ctcatcgaag atctcgatat ccgcatccac gatctgtaaa aagcgctcga taccggtcat
     8821 gccccgctgg aactgttcgg caaattcgat gatacgccgg attgttgcga tcagggtgga
     8881 tacatacagc atatatgcca ccaggtcacc agcggcgatc cgtccgttta ccataaaaat
     8941 accgcctgcc accaatacca ccagatacat caatccgtca aacagcttca ctgtggtatt
     9001 gaatgccgcc atgtaacggt aggtcagctt cttaatatcc agaaaagtgc cgttatcttt
     9061 ctcaaacttg acattttcca cctcttcatt ggtaaatgcc ttgaccacct tatggcccag
     9121 cagactatcc tcgatccggg cgttcagctc tccgatctgg ttacgctgct tgcgaaaggc
     9181 acttcgcatc cggaaattca ggatcatgca cacaccgcac atcagcggca cgcacagaaa
     9241 gatcatcaat gtcagcggca gattgatatt tgccagaatg ataaaagaga ttacggtctt
     9301 gattccggcg atgaaaaatt cttctgggca atgatgggcg aattccgtca catcaaacag
     9361 atcattggtg atccgcccca tgatctgacc gactttggta ttgttataat aagtattgga
     9421 cagcttctgc aaatggttgt atgcatctct gcgcatgtcc gtctcgatct tcgcgcccat
     9481 gacgtgtccc atatcggaca tatagtaact ggcgatgcaa tccacgatac gaagtccgaa
     9541 atacagaact cccagtgaaa gtatggtctt caccgacagg gacgcaagat cccgcagccc
     9601 ttcattggta atgtagcgca taattaacgg caacaccagt tcacagaccg tggtcagtgc
     9661 tgcacagaac agatccatcc acaaggtaaa acggtatttt ttaaaataag gtgcaaacct
     9721 gcgaaacaat tcgcgggtgg aatattgctt gttatccatt gttgtgttcc tttattttgg
     9781 tttcagagct ataggtattg tccttctccc acatagaagc gtacttttcc tttttgctca
     9841 tttttctggt cataaagtgt tttgtccgca gccgacagga aatcccacac tcggtttggt
     9901 tccttcggaa tagcacagac accacccacg gaaatcgtaa gccagggttc cacagaagag
     9961 tcttcattgg gaatctcaca ttcccggatg cccatacata gctgtcttgc atgttcctct
    10021 gcatactccc tgtcacgtcc cgtcatgacc accaggaatt catctccgcc gtaacgcgcc
    10081 acatagtcct ccggaaaaca ttttttcaga gtatccgcta ccgcgcagat tccttcatct
    10141 ccccttccat gtccatagcg atcattcaac tgtttgaaat ggtcaatatc caggaacaga
    10201 atgcccagtt ccttctcatt cagaagacag tcttcaaaca catcctccag ataacgattc
    10261 aggcttctct tattatagag tcctgtcagc tcgtcatggt ctgcctgata caacagccgg
    10321 tttctcttac gttcctgttc cttctgctcc tcttctgccg cctgtaatcg ctttctaagc
    10381 tccatcatat acatgacctg actgttttct gtcagctccc agctgtcttt caagtggaag
    10441 aaggtgtctg cgctctgttt gaattcttcc ggagtcattc tggaactgca ataacgcatc
    10501 cgcagagaca aaagctgcaa tagcagactc ttgttctgtt caatggcagc ctttggttcc
    10561 agacagttca atacttcctc tatctgttcc ggtttgccta tcttttccac atattgcagc
    10621 agattctgaa tactgtcgta ttcggaagag acctgtgtca tatcctctaa tgccattacc
    10681 gctgtacgta catgctcttc tgccttcttc cggtctccgt ttcgctccgc cagatgagcc
    10741 agaaatacat tgacacccag ccttgtctca cgtatgacct cctcgctctg cccggtctct
    10801 tccagtttac acagtacctt ttccgcctcg tcatccatat gcagatgcag caggcagcat
    10861 ccgtactctg ccagcatttt ccggaaattg atcctgctgt atgtactgtt gtcatccgcc
    10921 tgttcaaatt cgcggataca ttccgcatgg cactgcacag ctctttcata agctcctatg
    10981 agataatacg catccgacat attgctcagt acaatgctgt gcaccatctt atcatcgtat
    11041 ttttccgtat aatccaatgc tttatcgtac tgttccatgg ccagcgacag gttgttctgc
    11101 atatgtgcca caataccgat catattgtac actctggcta catgatgctc cagatccgtt
    11161 cccagaagac acctgacgct ctcatccaga taatgcaggc agccctcctg attccctgtt
    11221 gtcagttgat aaaaggcagc aaaatagcag atccatccgt ggattccctt atcatcctgt
    11281 tctgtcacag aggtaagtgc ctgctccaga gtctcccagg taaaaggaat tccgtctgtc
    11341 atctgctgat tcaggcgttc tgctgtctct aacatttgct gtctcgttgc gctcatatct
    11401 atatccatac cgtcctgtta ttctatgtca attccgcaaa tcacggttcg ccggttccta
    11461 tgcccagccc ggtgggcata atctatttta cttattattg tattatttta tatcgcagta
    11521 cgcaagtttt tttcctgaat aaaatgtttc ccaatctccc gcaaaaatgt taaaatggag
    11581 ttatatttcg taagctagta caaaaaggca tggttatttc tatggaaaat ataacagtta
    11641 ataaagagtt tcatgaactg cattttgaca gcatccaggc tttacgcggt ctggctgctc
    11701 tttttgtggt atttcagcat gtccgttttc tgaacttcgg agctttcggt gtggacatct
    11761 ttttttgtat cagcggattc atgatcatgt ttaccacgga aaaaagtacc aaatatttct
    11821 tccgcaaacg cctggtccgg attcttccgc tttactacct gatgacactg ggcacctatc
    11881 tgtcgctgtt gttgtttcct tccatgttcc aacagaccag acacgatttt tcctatctgg
    11941 tcaaaagtct gctgtttatt ccttttgata tcggcagcgg agcgatccag cctttggtac
    12001 ggatcggctg gaccattaac tgtgaaatgc tgttctatct gctgtttttc attgcctttc
    12061 acatcagcat gaaataccgc ggtctgatct gcagcgcatt tctggtgatt ctggtgggaa
    12121 tggtacagat atttgctccg gcgatctcat cctggcagtc tccgataggt aatgtacttt
    12181 ctcctttcct cattttttac ggagatcctg tcatgttgga atttctcttc ggaattctgg
    12241 cttactatct gctgcggtat ttgtatcgca ggcataccct tcatccggtt tcgcggacag
    12301 taagcctcgt atccgcggta tgtatcccgg taatcttttg tctgcttgct tactttacgc
    12361 cccggatcaa tgtgattggg ttcctcaggc ttccccagtg ggggctccct gctctgctat
    12421 tgttaatcct tgcttttctc gcaggcctgc ggctttccat gccccgcttt tttgtgcagt
    12481 tgggaaatat cagctattcc ctgtatctgg tacactacta tccggttatg tttctggacc
    12541 gtgtggtatt tgacttttcc accctatccg tattatctgt tgtcggactg atcgtaagcg
    12601 ttgtaataag cattgttctg gcttatctgt gctggatcct gatagagcgg aaattcaccg
    12661 gatggctgcg tagaatactg ctgcgttaat tgtatatgca atttacaaaa aagatgcaca
    12721 ctttttttat tgacatttcc ggagccctat agtatattat aacttgtccg taagatatgc
    12781 gcgagtggct cagcggtgga gcacctcctt gccaaggagg gggtcgcggg ttcgatcccc
    12841 gtctcgcgct ctcatgaaaa aaagaacgat accaaatcgg tatcgttctt tttttcgagc
    12901 cctgccaggg ctcgaaagtt cgatgtctac gctccgctcc ggtccgcgca gtccgaggtc
    12961 ccccggacct cgtgcgccgt ctcgcgcttc ttttatttgc agcggaagcc ttgattttac
    13021 agggtttccg ctctttttgt gtctgcatga aattgtagac agctgtagac agccgattgt
    13081 tataccacga aagacaatat aacgtttggt taataattgc atctgccaat ctgtctatat
    13141 gtacgaattt tatctctatt gcgtaccgac ctacatctat ccgcccatta cacgtagcca
    13201 cctactgttc cttgcttcat ttcaaaatct tcaccacggt cgctaggatc cacagtaaac
    13261 ccttcacttg aggaagagct accgcctccg gcggtactgt cactgttgcc agtggttcca
    13321 ccagtgatta acttgtctac taaatccgtc atatctcaat gataaaatga tttttctttt
    13381 cataaatact ccaatctgct acaatgttta tgagtttaga cattataaca caaaaataat
    13441 aaaattgaat atcgggttaa taatgatgtc ggaaggttag aaatgcgttg tagacagctg
    13501 gttctacaac gaatgtggtt aaaactaaaa aagccaccac aaaatggtga ctcaataaca
    13561 taagcaagaa accatccggc aggcgtcgcc tctcctgcat ctcttttgac ccatagggtg
    13621 cgtggttgca acgaaattta ccacctcgga tagttcctct cttattgtat atagattata
    13681 ccctgtttat tccctactgt aaagaatttc tttatttcgc aagtaattct gcaatcttct
    13741 ctcactaatt ttccagcatg aaatcacaga atttttatat ccatgttcat cccccgaagt
    13801 acataatctt aatacaatct gtaaaaatcc ttcttccgtt tgtattcttt taataatcag
    13861 tcccgtatat gcatgcttat ccctaataat ataatccggg gtacttattg cctcctgtat
    13921 atttttaata ctttattata tgcctctgga tgtctgtcct taatatgttg aatctgattg
    13981 tctgtgataa taacctcttc ggtaataata tcctctgtta tacatttata tattttttga
    14041 tttatttttc ctatgctata cacttcactc ttttcaccac cttcatttta acttcatgtt
    14101 tctttcttcc attcacgtct ctgggaaaac gactatagat cgctagtcaa cgatatgaga
    14161 atttgtaaga gaaatattgc tcttattgct aagataacat gaacggccat ttttacaaga
    14221 aaaaagacga ctgcactcac atgcaatcgt cttctatctt ctttactgtt ccagtttatc
    14281 cgcatatttg atacggtaga ttctccgcag ggaatcattg atgcgttcct cggaaatggt
    14341 gccgttctgt acagcctcca atactccgtt gtatgccgtc tcaaaattct ccggtgcata
    14401 gatcatatca cagcctgcgc gaagtgccag taccgcagct tcatctgctc catagtaatt
    14461 ggagattgca gcctcattca tggctccgga cacgatcaca ccgcggaaat tcagttcatt
    14521 tcttaaaata tccgttacca ccgcgctgga gaacgtgcag ggggtattgt ctccggtcag
    14581 ggaagatgac gccatattac tgatgcggat cattttggtc atgccattgt cgatacaagt
    14641 ctggaaaacc acaaattcat tggctctgaa atcttccgca cttctgtctg tagatgccat
    14701 gccgtccttc gtgctctggg tactgctgcc gataccaggg aactgtccga tacatgcggt
    14761 cactttctgc tcctgtaatc ctgcctgcat atatcctaca aaggatgcca cattgtccgc
    14821 ttccgatccg taagaactgc tgcctgccgc atttccatcc actacactga gatctgcaga
    14881 aggtgcgaaa tcaagattaa agcccagctc tgccagataa gttcccaccg tggttcccgc
    14941 taggtacgca ttgttggtat ctccggtagc cccgatgctc tggggtgtgt ccactttagt
    15001 gccaaggcct gccgcagcta ccgcatctgt atcgcttccc tctccgtcaa tggcaataaa
    15061 caggggatag ctggtatata atttggtatt atcgatcatc tgcttcagct gatctgcaga
    15121 ctggatattc ttcgctgcat atacgattcc acctaccggg tatttcgaca aagcatcctt
    15181 ggtaccgtct cccgcctgta ctgcagcaga cacaccggtg atcgattccg gcgtcgtaat
    15241 gaataatccg gctaccttat cttccagagg catgttctgg atgattgcct cattgataat
    15301 ggtatccagc ttctgctcat ccgtgagttc caccactgtc tcctggcttt ccgtcggtgc
    15361 ctggattgtc tcctcatccg aaaggatctc atccaccttg ttctgctgtt cttcctgatt
    15421 acgactcatg ctggtaatct tgcttacagc aaaagcaatt cccaccgcca gcaacaggat
    15481 cataatacct acggccgtat atgcaatgat ctgatttctc tgtcttctct tatgtctgag
    15541 ttctcttttg tcctgctgat tctgttcgtt tgtgtccatt ttcataactc ctctatctgt
    15601 actattcttc tattgtacat gatatcggta ggtttttcca ctggaaaatt gtacaactat
    15661 tcagaacctc attcgccaaa taaaaaatcc aatatggact tacaaatgca agcatttgac
    15721 gaccatcttg attttttact tggctctgaa tagttataca acattaagag agcgtaccga
    15781 tcattctttc aatcagtacg ccctctcaga ctacaggctc caatggcttg ccctctcttt
    15841 cttaaatttg atcttccatt ttgtcttttg ttctctttcc acttttcttt tgtacgtatg
    15901 tactgtaatg tgagataatt cttatcttct gttgttcatt actttttctt ctgtgtactt
    15961 gatgttctct atgtaaattg gttctctttg gtagttctat tcagttgtac atcatggaag
    16021 actgtggtag gactttaatt gttaggtgga ctgtacctct ctttctatag attcttttgt
    16081 acttctctcc caaccttctt tgttgggttg ttccgtttcc cttttgttaa gtctaatata
    16141 atacatgctt gtacatttgt caacacattt cttgaaatat tttaatttat tttcaatatt
    16201 tttattgtac tggtatattt ttacattttt cgacaatttt ctatatattt tctgtctttt
    16261 ttgaaagaga atggcaaggg caaagggcgt gccgctaccg gggaaagtgt aacgagccat
    16321 gccagcatcc gccaaaagcc ctgacgggct ttcggcggat gacggctgtc gccgtataaa
    16381 gtcaaaaaac taccactccc tggtgagcaa gctccccatt gagtggtagt ttccttgcct
    16441 ttgcatgtct cgttacacgg caaactggct attatacagg ttcgcgtaga atccgccttg
    16501 tgccaggagg gtttcatggt tgcccggttc gatgatattg ccgtctttca ttaccaggat
    16561 gacatcagcg ctctggattg tggagagtct gtgggctacg ataaagctgg tgcggccttc
    16621 catcatcgtg gcaaatgcat tctggatctt gatctcggta cgggtatcga tggaggaggt
    16681 tgcctcgtcg aggatcagca tgggaggcag gcacagcatt actctggtaa tgcataagag
    16741 ctgtttctgt ccctgggaca ggctgccgcc gtcttcggtg atcacggtgt catacccttg
    16801 gggcagtcgc ttgataaagc tatgggcatg ggatgccttg gctgctgcga tgatctcttc
    16861 ctcggtagcg tccggtttac ccatgcagat gttgtcacgg atagtaccgg aacgtaacca
    16921 ggtctcctgc agcaccatac cgtaattggt acggagactt cctctggtga tatcccggat
    16981 atctgtgccg tccactttaa tggagccgga gttcacatcg tagaaacgca tcagcagatt
    17041 gatgacggtg gtcttaccgc atccggtagg gcctacgata gcaactctct gcccgggttt
    17101 tacctgcagg ttaaagttct ggatcagctt cttctccggt acatactgga aatatacatc
    17161 cttcagatct acgttgccct gagcctcttc taatactctt gcatccgcag catcgggcac
    17221 ctgaggctcc tcgtcgatca gttcaaagat acgtccggca cagacaaaag cattctgcaa
    17281 ttctgttacc acgccggaga tctcgttgaa gggcttggtg tactggttcg catagcttaa
    17341 aaagcaggac agacggccta cgctgatacc gcccttgatc gctacgagcg caccgaacac
    17401 accgacaccg gcatatacca gactgttaac gaaacgggtc gccgggttcg taatggagga
    17461 gaaaaagata gctttcagag agcatttctg caatctgccg ttgatctctc caaacttgct
    17521 cttcaccgct tctcctctgg aaaaggtatt tacgatcttc tggttgtcga tcatttcctc
    17581 tatcagggag gtctgctcgc ctctggtctc ggactgctcc ttgaacatgg agtaggtctt
    17641 ggttgcaatg aattttgcca ccaggaagga taccggagtg atacatacta ctaccagtgt
    17701 gataggcaca ttggtcacca acatgaagcc cagcgtacac aggatcgtca gtacaccggt
    17761 gaacagctgg gtaaatccca tcagcagacc gtctgcgaaa gtatccacgt ccgcaataac
    17821 acggctcacg atatcaccgt aagcatgggc atccagatat tttaagggca ggatctccag
    17881 cttcgcaaaa gcatccgtac ggatatcccg gatcacatga taggtaatat agttgttgca
    17941 ggtgttcata acccactggc ctactgccgt gatgatcatg acgatagcga tttttttcat
    18001 aatggcaaag atgtcgggca tatccacctg tccctcgccg atgatcagat ccaccgcatt
    18061 accggtgagg atagggatat ataaggtcag taacacggtc aggcctgcta acagtaggga
    18121 gagtaccagt gccggtgtat atttccggat atatttcaat acccgcttca tcgtctcttt
    18181 gttatttttc ttctgcatta ccgaacacct cctctctgtt cagggtactg ggaataataa
    18241 atctcctgat atacggtgca gtcctttaac aactcatcat gggttcccat acctgcgatc
    18301 tctccatcat ccagaacgat gatcttgtcc gcatggcgga tcgtggaggc gcgctgggat
    18361 acgataaagg ttgtggtctt atcttccaaa gtccggattg ctgcacggag ttttgcatcc
    18421 gttgcgtagt ccagtgcgga ggcactatca tccaggatca gaatctcggg atttctgacc
    18481 aaggctcttg cgatcgtcag acgctgtctc tgtccaccgg agaagttctt accgttctgt
    18541 gccacagttg catctaactt tccgggctta ccgtctacca cttccgatgc ctgtgccagt
    18601 tccagtgctt tccacatctc ctcttcggta gcgtctggct tgccccactg gaggttgctg
    18661 cggatcgttc ccttgaacag gactgccttc tgcggtacga cgccgatacg tccgcgcagt
    18721 tcctcatcac tcatggtcct gatatctctg ccttccaaca ggatctcacc ttctgtcgcc
    18781 gggtagaaac cggggatcag gttcaccagg gaggtcttac cggaaccggt acctccgatg
    18841 atacctacgg tatcgccacg gtttacggtg aagttcatat cctgcaaagt aggtgctccg
    18901 gctccctgat aagtcaggga tacatgtttg aagtctaaga aaggtgcgct cttatctacc
    18961 ttatccgcca gtttctgatc ctgtactccc acatatacag catctgcgcc aatatcgaaa
    19021 acggaagcta cacgttctgc gcaggccaga gccttggtca tggttacgat caggttcgcc
    19081 agcttcacca gctctaccag gatctggttc atatagttga tgatcgctac aacttcaccc
    19141 tgactcaggt tgccgatgtt gacctgtacc gcaccggtat agatcagggc aatgatagca
    19201 ccgtttacga tgacataggt cacaggattc atgcaggcac tgatcttacc tacgaagatc
    19261 tgcatggcgg acaatgcctc attgttctcc cggaatcgct cttcttcctt cgcttcctga
    19321 tggaatgcac ggatgacacg gacaccggtc agattctctc tggtgatgcc caatacctga
    19381 tccagcctgg actgcacctt tttgtacata gggattgtgg ataaaataat accgaatacc
    19441 acaatggaca acaaaggaat cgccactaca aagatcaggg cacatttcac atcaatggtg
    19501 aatgccatga tcatggcacc aaaaacaatg atcggggaac gtaagaacag acgcagtgtc
    19561 atgttgatac cggactgcac ctggttcaca tcactggtca tacgggtgat catggtggaa
    19621 gttcccgcct tatcaatatc cgtaaagctc agatcctcaa tatgatcaaa caatgcctga
    19681 cgcagcttgg tagaaaatcc cactgccgcc ttggctgcga aaaactgcgc cgtaatggac
    19741 gacgccagtc ccactacacc aaggagcagc aggagcagtc ccattttccc gatatacccc
    19801 atgttccggt tggaaatacc atagtcaata atatttgcca ttaccagagg aaccaacagt
    19861 tcaaaaaatg cttccagcag tttgaacagt ggtgccagta tggactcttt cttataatct
    19921 ttcagataca tcattaatct tttcataaag tcctcatttc ttcgcgcttt ccatgtaagg
    19981 cgcatccgct tttcatgctt cctatccctg tacagctttc ccgttcccta tcttttacgc
    20041 ttctgctgtt ccgtaacaga ccgccagcga atagctatcc tccagaaccc gttcccggaa
    20101 acatatctgc ttttcctgcc agttctgttc gtcggagaaa tcttctccca ctgcactgct
    20161 gatcccctgg ccggtgtatt tgccgtaggc ttcttttctg ctccacaatc ggtaaaacag
    20221 ttccgtgcgc tgtgacgcag gcagttcctg cagcagtttc cattccgttg gcgcaaaaaa
    20281 acgctctgcc gtccgttcct tatagggaac ccatttctga atgtccgctc ccacttctcc
    20341 gtctcctacc gcgcagacca catagtctcc ggaatgagac agattgaatt ttttcgggat
    20401 attcacgatc tgtggcttcc ccagttctcc atattcgtag gaaaaggata acgcccccgc
    20461 cttgtcccgg tgcatccgct cctgtaacac cgcaagcagc tcatcctctt ccagaaacag
    20521 gattccggta ttctccgtac cgtctatcag atccagagcc atcttctgca acagcattcc
    20581 cgcaccgaga ctggctgcct ttgctccggt gcttttcagc cgttgtacct tcttacaccg
    20641 caccggatcc acatattctg cagcttcccg caggaatgtc cgcctgattt cttcctcacc
    20701 tgggtgcaga tgcaatacat aaattcctac agccatattc ttctcctgac aaattgccat
    20761 atatacccaa cataaaggct gtcacacaga aatcgtgcga cagcctcatt attattcttc
    20821 cggcttggcg gtgatggcca gatgcagttc atccaactgc ttctgctcta ccgtacccgg
    20881 tgctcccatc ataatgtcca tggcattctt gttcatgggg aaaggaatga tctcacggat
    20941 gctttcctca ccacagatca acatgatcat acggtctacg ccgggagcga ttcctgcatg
    21001 aggaggtgct ccgtaacaga atgcattgta catggcaggg aactttgcct tcacatcggc
    21061 ttcgcctaag cctaccagtt cgaatgcctt gatcataatc tcaggatcat ggtttctcac
    21121 tgcgccggag gacaactcta cgccgttgca gaccagatcg tactggtatg ccagaatctc
    21181 caaaggatcc tgattgttca gcgcatccat accgccctgg ggcatggaga acggattatg
    21241 acagaattcc agttcgccgg actcttcacc gatctcatac atcgggaaat ccacaatcca
    21301 gcagaactcg tagcaatcct tcttcatatg tttctcgctg gttgcaccca gcagctttct
    21361 gtatacaccc gctgtcttct gcgcttccag cttcttgcct gcggcaagcc ctacaaagtc
    21421 accgttctta aggccgagag ccttgatgac ttcttccttg cgatcctgca agaacttgga
    21481 gataccgcct acgaactctc cgttctcgtc tacacggaac cagaatccct tgcgtgcggt
    21541 ctgtacttct acctctgcgc agatagcatc gatctgctta cgggtagcag tgaagccatc
    21601 caccacgata gcctttaccg tctgaccttc gaaagcagca aagccacagc cctgcattac
    21661 ctcggtggca tcggtcagta ccagatcgat acgcagatcc ggcttgtcgg aaccatactt
    21721 gtccatagcc tccagataag ggatccgtac aaacggagcc ttggaagcat ttttataaat
    21781 accgtacttt tcaaatacgg gaggaagtac ctgctctacg atggagaata catcttcctg
    21841 agaagcgaag gccatctcca tatccagctg ataaaactct ccgggagaac ggtctgctct
    21901 cgcatcctca tcacggaagc agggagcgat ctggaaataa cggtcaaaac cggaagccat
    21961 caggatctgc ttgaactgct ggggtgcctg gggcaaagcg tagaacttgc cgggatgatt
    22021 tctggcagga accagataat ctctcgcacc ttccggggaa gaacaggtca gaataggggt
    22081 ggtgatctcc atgaagttca gagcatacat tctctgacgc agctctgcca cgatctgact
    22141 tctcatcacg atgttctttt tcatgttcgg atttctcaga tccagataac ggtatttcag
    22201 acgggcattc tcgtctgcat ccttggactg attgatctca aaaggaagtg cattgtagat
    22261 acacttacct aaaatctcga tcttgtcggg cactacttcg atctctccgg taggcagatt
    22321 ggcattcttg ctggaacgct ctcttaccac accttccacg gagatggtgg actccttgtt
    22381 gatgccatcc atcacgttca tcatctcttc ggtctctact accagctgag tggttccgta
    22441 gaaatcacgc aaaatgacaa atccgagatt cttgctgact tttctcatgt tctcgaacca
    22501 gccgaccagt gtgactctct cgccaacgtt gccgatccga agttcattac aattatgggt
    22561 tctggactga atcattgctt ttgctccttc gtctgttgct ttttatctct ttaattcttc
    22621 tttatagaat tccacgaatt cttcgtagcc ttccttgttc agctgttctt tctggaattt
    22681 cttattcttg ttcatgcggg ctaccagtac ctgagtaccg tccttacggg cttcctgggc
    22741 ctgtgcgatc acttcacaca gtctttcacc actgacgccc ttttcgatca ggtatgctac
    22801 ctgcttcggt ctcgtaggaa ctttgaatcc tctctccatg agcagcatga tgatacgctc
    22861 gaaaccgatg gaaaatccac aggcgggtac atcattgccg gtgaacttgc ctaccatctt
    22921 gtcataacgt ccaccaccgc cgcagctgcc gccgaattcc ggcatggcga tctcaaaaat
    22981 agttccggtg taataggaca tacctcttac cagagtggca tcgaagacaa tctcgaaatc
    23041 agaagccttg gtggctttga cgctgtccat gatctctctg aggctctgag cgatctccgg
    23101 ctccaggaat ccttccagtg tatctgccag ataggccaca ccatcctctg cactctccag
    23161 tcccttgaac agttccagat acttgtctac acatgcctta tcaaaaccgt tctccaaaag
    23221 ctctgcttcc acgccttcca gaccgatctt gtccattttg tccagagtaa tgaatacact
    23281 gtcataggac tcttcgggga atccgctgta agcagccatt gccttcagga tacggcgttc
    23341 attgatacgg atctggaaat tcttgaatcc cagcttaccc aatgtggtgg tcgttgcaag
    23401 gatcagttcg atctccgcca gattcgaagg ttctcccaga atatcgatat cacactgcat
    23461 aaactgacga tatcttcctc tctgaggtct gtccgctctc catacattgc ccatctgcaa
    23521 tgccttaaaa ggagaaggca gggatgccgc attattggaa taataacgta ccagcggtac
    23581 cgtcaggtca taacgcagcc cactgtccac tacctcttcc tcggtggtcg cagctgctac
    23641 attcagtttc tcaccgcgct ttaagatttt gaagatcagc ttctcattgt caccgccctg
    23701 cttactgctt aagttcgcaa tattttccac acagggagtc tcaatggaag taaaaccgaa
    23761 tttgccgtag gtgtccttga tgacgctgat cacgtaatca cggatctgca tctcctccgg
    23821 caggatatct ttcatgcccg ttgtaggctt cttgctaagt gccatggttt tctacccttc
    23881 cgctcgtata ttttatatct gcaaattgcc tttcccgcaa ttttagctca atatcttata
    23941 tatgatactt tttttcccga taactgtcaa ccctcccgtg ccccgcttct gccagaaatt
    24001 caagcatttt tctatagaaa aacaggacag tgatctctca ctgtcccgcc ttctctttca
    24061 tcttcttcaa ataggcatct ttcaatgcac atttttcctc taactgacgg atctgcttct
    24121 tcagatcctc cagatattcc gcggtgactt tcacgcctcc gcggaccgct acatcttcat
    24181 gtctgctcat atcctccagc cattgtctga tggagttttc ccgcagatca ttatacgtat
    24241 tgtcttcctg tggctgccac ataatttccc tccaaactta ctcagacacg gaactgctgc
    24301 atattctgct gaagtttgcc gatgctctgt aatacgccct tggtctctgt agacatattt
    24361 tcactggaat aggtgatctt ctgaaggttc ttattcactc cggccaccgc atccgccagt
    24421 tccgcctgca tcgtaattta ccctttgatc gttttcccca tagtgcacca tcccttttat
    24481 ccttattttg ttttgcgtac catagagtac ttttctctta attgtacttt ttctcataca
    24541 atgtgtcaag aaatcaccgc gtcaaatcac caaagttttt agttgcagaa aagctgctac
    24601 ccacgatcgg ataacagctt tctgactgcc tgttatgatt ttcagactat tttttaatat
    24661 ttgatctctt tgccttccag tctccactgt ctgaactctg cggatgcgga cagtaacagg
    24721 tcggaggaag aattcagtgc ggtctccaca gaatcctgga tgacaccgat gatgaagccg
    24781 acacctacga tctgcatggc aatatcgttg gagattccaa acaacgaaca tgccataggg
    24841 atcagtaaca gggatcctcc tgccacgccg gatgcaccgc aggctgccag ggttgccaga
    24901 atactcagca cgataccgct taagaagtct acgtgaatcc ccagagtatt tgccgctgcc
    24961 agtgtcatta cggtaatggt gatggctgca ccgtccatgt taatggtagc tcccagcggg
    25021 atggttacgg aataggtatc cttatccatt ccccactctt cacaggcctt catattcaca
    25081 ggaatattgg ctgcggagct tctggtgaag aacgcagtaa tggcactctt cttcaggcac
    25141 ttgaagatca gcgggtacgg gttcttgcgg atacaccaga acacgatgat cgggttggtc
    25201 acaaagtaga taaacagcat ggaacctacc agtactaaca gaagttttcc gtattcggag
    25261 aagatctcca gtccgttgga agaaatggag ctgaatacca gacctaagat tccgaaggga
    25321 gccatactga tgatccagct gactacctgg gacagaccgt tggaaatgtc atccagtact
    25381 ttcttggtgg tatcattggc tgctctcata ccgacaccga tgatcacacc ccaggtcaga
    25441 acaccaatat agttggcatt taacagagag gataccggat tggctaccat attcattaac
    25501 agagtcttca gtacttccac gataccgtcc ggcgctgcca tatcggtggc tgcgtctgcc
    25561 agtgtcagtg tcacggggaa taaccggctg gtgatcacgg ccaccagcgc tgccagaaag
    25621 gtactgaaca tgtacataat aataacggtc ttaatgatgc cgccatggct cttccctgca
    25681 tgacacaagg cactgatcag caggaagaat accaacaaag gtgcaatggc cttcagggca
    25741 cctacaaaaa catctcccag aatggcaata ccggttgcct gcggcacgac cagtcccagg
    25801 atcacaccaa tgaccagacc acatacgata cgcaggatca ggctgatgct gttccacttt
    25861 tcaaatagtt ttttcatact tacactcttc tttccctttt ttctgatgta tacatgtact
    25921 tcattgcaca cattatgtgc tattatatag tatttctcat ataaagacaa gtgtattttc
    25981 cccacggaca ttttgaggct atttgttttt cgatttttag aacagaaaaa agcggatccg
    26041 caaaaagcag atccgctttc ctcattttcc tattacatta caaaacattt gtcattgcgg
    26101 agattcccac cttcatgaca cccgccagta ccatgaccca gataggattc agtttcgttt
    26161 tccgaagcag caacacacag attccgaaga tcaccaccat gctccactcc gtacccgtca
    26221 gggaaatcgt ccctccgctt ccccagaacg catttttcag gatcaggatc cctgctgatg
    26281 cgatcatggc caccactgca ggccgcagag atttcagcac tccctgcagc agatccagat
    26341 tacgatactt caggtatagt cttgcgagga tcgtcacaat gatacaggac ggcagaatac
    26401 atccggccgt cgccaccacg gctccgggaa tcccggcaat cttcagtccc acgaaggtag
    26461 cggaattcac agcaataggc ccaggtgtca tctgtgatat cgtgatcaga tcggtaaact
    26521 ccgacatggt cagccatccg tgagtcgtga ctacttgtcc ctggatcaga ggcatggccg
    26581 cataaccgcc gccgaagctg aacattccta tttgcagaaa acttaaaaat aattgcagat
    26641 agatcatttt ccagacctcc ttttggaaag aagtgtccgg accaaaccaa tcagtccgca
    26701 ggcgatcaca atgtagacca cattgatctc cagaatacat gctgccaaaa aagctcccag
    26761 cataatgaca agtgacatag gatctttttc ttttacgata ggtgcaccca tatcatacgt
    26821 cacggaagcg atcaccgctc ccactcctgc ctgcatccca gacagcatct gcgccacgaa
    26881 gaagttactg cggaacacat tgtagcagac agatattgcc gctataatca caaagggcgg
    26941 caggatcgtt ccaaggatcg ccaccagtgc ccccgggatc ccggccagct tgtaccccac
    27001 cacgatggct ccgttgactg cgatcgcgcc gggcgaagac tgggcgatgg ccaccagatc
    27061 cagcatttcg tcttctccga tccaatggta ttcatccacg aatttcttct tcatcagcgt
    27121 aacgatgaca taaccgcccc cgaacgtgaa tgcactcaga tacagtgtgg acaggaatat
    27181 tttccacaat atttttccct gggattcttt ttcttccctg ctttccatgc tacttttctc
    27241 cattcttttt gacagtgcct tcccgcatcc ttgctgttct gtctgtcaac cgtaaaactg
    27301 caccgcattt ttcaatgctc cacacacaca acagtatctg ggtgtaaccg aaaaacaccg
    27361 ctggagtccc gatcagcgtt accagcccca gtgcccagct tcccattctc tgtacaagat
    27421 cccacgggaa ataccgcaga gacattacag tgagcaatgc cgccgctccc agccagatat
    27481 gatcattttt ggcgcatatc ttttttcctt ttacaaagct ttgatagagc aaaaacaaca
    27541 gacagccaag cagcagaatt cccattccgg ggtgtccgtt cctataaaaa taagtactga
    27601 acagaccgcc gatactgtat ccctgctcca tgatgctggt atattccaat ccgctctccg
    27661 caaaggaccc gtcgaacagc cagtgtttcc acgtcatata ggtggggtat gccatggcaa
    27721 ctcccagaat tccgatcacc ggatattgcc attgtctccg gcagatacca cccacgcaga
    27781 tcaggaacaa aagcgtcaac gcggctacgc catccagtct tccgataatc cccagggcca
    27841 gtactgcggt caatccgtag cctgtcttcc agaacatttt ctccgtatcc gacattttta
    27901 ccagagacgc tgccaggatc ggcaccacca tccatatcag aatctgtgac cgatccacac
    27961 tttccgttac cacatataca tgataaggca gcaacagata ccccaatgcc cccaccagtg
    28021 aaaaccagtc tttctttttt ctctttaaaa cgcaatggaa catactccat gcggttccaa
    28081 aggtaataat ctgggtcaat atgataaaag tactttccat ctgttattct cctgtcatga
    28141 aaggttcttc attgtaccag tactgaacat ccgattgcag tataatggta ttttcataat
    28201 ataccacctg taaaacaagt attaacagta ccgcaaacac gatccaaggg gaaattttgt
    28261 tctggctttt tcttttttga agaagtatcc ataccacact gcccagtact gcatatccca
    28321 acacacagac aacaaacttt ccgggatttc ccattacgta taaggtcttc accgccagag
    28381 gattccatat accaaaagct gctgccgcca acaccagtcg cgcatccttt ccccatttgg
    28441 aaaacaggaa ataaagcaat gcaagtgcag ctccggataa aaatgctatc atgatcttat
    28501 aagatgttgc gaccggaaat ccaatccacc gcattcctgc cggaatccat aaatatagta
    28561 aatgggctgc cggaatcctt ccccattctc cctgaagcat atactctgtt tctctcaggc
    28621 actgtacact gtcagtcccc agaacaagat aatccaccag caatgggata caggctatca
    28681 tccagattcc caccattgca cagttgatcc attttttctg tccgctcact tctttacaca
    28741 atacttttct gcggaatatc gcaattccat ccagacaggc aaaaaaaacc agcactatta
    28801 caaaaagaat ccttcttccg gcggttgtct tctctacttt caactcatag gaaacatttc
    28861 ctccctccga aaatggctgt atctgcagat gtgctgcatc aaccgtatct gccacataat
    28921 aggtaatcct tttatgctct gtatctgcag acaaagttgc ccggttgccc ctgatcgcac
    28981 gaaaagtggt ctcctccgct tccagactga tctccattcc gctttcgatt ccgccatttg
    29041 caatatccga ctctactatg atgcgatata ctcccgggtt caatgcaaaa tctttgctga
    29101 cgatcccccc tgtttcttcc acccgataca attgctgatg atctgcctca aatacaatat
    29161 ccgatccccc aaaagacagc acgaacagaa ccgctaagag aagcccatcc gctaataaaa
    29221 tccatttctt tttcatctct gtctcctctt ccgaatcccg aaaaacacga tacccgctgc
    29281 tgtgatcaaa gtaaccagtt ccgccgttct ccaataccac ggttccacaa aacgcactct
    29341 gactgttccg gcatatccac ccggaatgca cacttgcaaa atatgtccac cgtcgtctgt
    29401 gatctccaat acttctcctt tgtcatccct ggctgcatat cccttataca tgagaaccgg
    29461 aagtgttact atttcctccc gggccgaatc attcctgcag aaaaagctta tatttaaacc
    29521 tctcttttca tagtctgcga tctgaatatt ctcattggct tccggtcttg caaaagtcag
    29581 ctttgtgctg tccgttccat agatcagata ctcttctccg gagatgtaac caaatcccat
    29641 actttcctga ttatataatg taacgtccct ctcctgtact cccgaatcca tcataaacag
    29701 ataggatgca gacaaagaca ctactgccgt gatcagactc atctgataga atattttccg
    29761 gttgttctga aaatatcgaa tcacatatcc cgttaccgtc accaacagaa ccgtacccca
    29821 tcccagaaaa cgattgggga actgcagact gctcaccagt gtcgctgcaa tgccggaaat
    29881 tttctgaatg ttatcccagg gaaaggaatt tgtggacatc cataatgcaa ggcatccgaa
    29941 tcctgctgcc tttatcgcaa agctcttctc agcctctttt tgtttttgca aatctcccaa
    30001 aaataacagc agccagaaaa tcatcattcc tattaccaga aacaatccta ccccaatagg
    30061 attcagtaaa tcccctgcct tgccttccat ggattccgga attctgctga accagaactg
    30121 ctgtaatatt tgcgcaaaaa tggttcctct gtcctggata gttctcgccg atgcatggcg
    30181 tatcctgaca tcctgtgtca aataatagtc aacaaacggt accagatacc acaggcttaa
    30241 tcccagcgca aaaaatgctc cactcgcaag ctgccgaaat ctttgccatg caaatactct
    30301 gcggatataa atcagacaga acagcactgt gaacaatgcc gtaatctcac aggtcagtac
    30361 atgggtctgg atcaatccgg catatcccag tcccaaaatg atccagcttt tatgaaactc
    30421 tctgtctttc acatcttttt caaagacaag ataaattccg tataccacca gcggcagaaa
    30481 cgtataggcg cttccctctc ctacagctcc taccattacc agtttaaaaa atctaaatat
    30541 ggataaagta tacaatgcgc tgcagaccag acctatgatc cggtctttga atattccgtt
    30601 aaagcagtac caggctatcg ctgccgtagc aatattaagt cctatgcaat agaaagcata
    30661 gctttccgtc atcgtaaagc ctgccatgcg cagaagtgcc ggaaaataca ataacagatt
    30721 acaatagaaa ataccattgg catagccgtg gtcaaatacc catctgggtt ccagccgtac
    30781 cggaaactgc ccagtcagaa gtccatcctt tacaccctca atgcgatgta aatggtaggt
    30841 cagatctgcc cctgacacca taccgtcata aaaatagggg atggacgcta aaaaggcgat
    30901 cacaccgatt ccaaacagga tctgacgtct ctgctcattt ctgccattta ccgcctcccg
    30961 acgctccagc cacatggtcg caagaaccaa cagtgctgct gccgcaagga tcacaagata
    31021 tcttgtccac agaagattcg tctccacgat ccgcagattc ccggtggtca gcttttcctg
    31081 tccggaataa tcgattgtca ccgtcagttc ttctgttggc tcataaagcc agaaatcata
    31141 ggaagtatgc cccagtgcac tgtacagatg ctcgccgttg cacaacaggc ctccctgata
    31201 cacagtaccg tctttcacat tgcagaccgc gatggcatcc ccggtgcatt cgtaggacag
    31261 ttccacccgg tatactcctg tccccagcga aatatgctca aataccggct cttctgtata
    31321 agattcccct tccgcaaaag tatagcttcc ggtatagttc cattcccggt tgggcatggc
    31381 aatgcggatg atacacccca ccatcaatgt agccagaatc cccagaatca catatctgat
    31441 ataaggtttt ttcttcacct ttcccattct cctttggtca catattgcaa atcatttgaa
    31501 ataaaacgca aaaatccata atgagtatag cacaaaaaaa agataatgaa cactctcttc
    31561 attatctttt tcctgtattt atgcgattcc tatgcctgtt ttgtatgatg ttccacatgt
    31621 tcgaaggtga atacattcag gaactgtttt gctctctctg tctccggatg ttcgaaaaat
    31681 ttatcgggag cggcctcctc cacgatctta ccctggtcga tgaaaataac tttgtccgct
    31741 accgcctttg caaactgcat ctcatgggtg acaatgagca tggtcttgcc ctgttttgcc
    31801 agatccagca taacatccag cacttctctt accatctccg gatccagtgc cgcggtcact
    31861 tcatcgaaca gcaggatctc cggatgcatg cacaatgccc ggacgatggc tacacgctgc
    31921 ttctgtccac cggacaactg tctgggataa ctctttgcct tatctgccag tcccacgcgc
    31981 gccagaagct ccatggcctc cttctgtgcc tctgccttat cgcgcttctg taccttgacc
    32041 ggtgccagcg taatattgtc cagtaccgtc agatgcggga acagttcata gctctggaat
    32101 accataccga tcttctgacg cagggtggtg atatttttac tgcccttgcg gatatccatg
    32161 ccctgcagct ttatcgtacc gccctgaata ggttccagtg cattaataca ccgaagcagc
    32221 gtactcttac cacagccgct gggacctacc actaccagaa catctccctg ttttacttcc
    32281 agattgatgc cgtccaggat cttctggcct tcatattctt ttgtcagatt ttctatcttc
    32341 aacaatgtct ctgccatgtt ttcactccat tctcattccg actccggttc catttctaat
    32401 tccacttttt ctccagcact ctggccagaa cactgaaggg ccagcaggcg aagaaataca
    32461 gaacaaatac cacaaggaat actccgaacg cagcattggg actgctcata cgattggcct
    32521 caatgatctg ctgtgccacc ttcagcattt ccaccacgcc gatcatgagc accaggctgg
    32581 tggtcttgat catacgggtg atcaggttga tggacagcgg aatcagtctc cgcacagtct
    32641 gcggaataat gatatacaga tacgtctgta ttttactgaa tcccagtgct tcactgcttt
    32701 cgtactgatg cttcggaata ctgatcagcg ctccacgcac cagatctccc atctctgcgg
    32761 tgccccacag gacgaagacg atgacggatg ccacctctcc ggacagattc cagcccagtg
    32821 ctctggtaga tccgaaatat acgataaata aaagcaccag ctgcggcatg atacggatga
    32881 attccagata gacccggaag atagctttga cgaaccggtt cttcaccgtc atcaacgctc
    32941 ccaccagaat acctaacggc atactgatca gaatagatac gatactgatc ttcatggcta
    33001 cccataatcc ctgcagcaga cggagcatgt tcgtgccttt gaataataca tcaatcccca
    33061 aatccggcat aacgcatcct cctctccacc agagatccca cgatcgatac cggcagcagg
    33121 atcaacagat aaaataccac cagaaggaaa aggctctcgg tagtcttata atacaggccg
    33181 atcagatcct ttgccgtaaa catcagatcc atcagactga tggcactgaa tacactggtc
    33241 tccttcaaca ggaaaataat attcgccaca aatgccggga tactggtaga catggcctgg
    33301 ggcaggatca catatcttag tacctgtccg gagttcattc ccaggctgat ggcactttca
    33361 ttctggatcg gttccacggc ctccagaccg ctgcggaatg cctccgccat ataactgcca
    33421 cctaaaaatg ccagtcctac cacaccgcag acctctgctg acatccggat cccaatcttc
    33481 ggaaatccgt aataaataaa aaacagctgt accagaagcg gtgtattacg gctcagttcc
    33541 acgtagaccg ctacgatcca ccgaagcacc ggtactttat aatattgtat cgccgcacat
    33601 accagtccca gcagaatcgc aaacaggatt ccgatgacac cgatcttcac ggtgagcaac
    33661 gctgcctttt catacatagg cagatatttt cctataaatt cccagttcat gtatttcttt
    33721 cctttcagga agcttttcaa caatcgtaac tattcagaat agttacacac aattttttag
    33781 attataacaa aagaagtagc cagatgcaag aaaagactgc ggcggcaaca tacgtgcccc
    33841 gcagtcccct ctctatcatt ctattccgat ccgtacttat tgtgcggtta ccttgccgcc
    33901 ttctactacc aggctgtcct cgtaatccag accataggta tctgccagag ttgcctcgta
    33961 atctgcatgg aagaagttct cttcaccaag gcttgcgatc tcatcgttga tccagtcaag
    34021 cagagtagta ttgcccttag atactgcggg agcgatggta tcagcgctgc ccagggaagg
    34081 aatacctacg gtatagcctt cattctgcag agcaaatgcg atgacctcag tgttgtcgtt
    34141 tgcccatgct acggaagtac cgttttcaaa agcttcctta gcggttgcat aggaatcgaa
    34201 cttctgtaaa ggaatgtccg gataattagc aatcagatag gtctctgcgg tggttccgga
    34261 gatcacaata atagaatcat ctgcattcca gttatccaga gtggtgatta cattgtcatc
    34321 cttggatact acgccaagtg ctacattcat ataaggagca gcgaaatcaa cttcctctgc
    34381 tctctcggga gttacggtga agttagctag gatcacgtct accttaccgg tctgcagata
    34441 ctcgatacgg ttggccgcct cggtagatac gaagttcaca tcgacaccca gatcctcacc
    34501 gagtcttctt gcataatata catcatatcc ctgatactcg ccgttctcat ccacataacc
    34561 aaacggattc ttatcggaaa atacaccgat gttgatggta ccgctttcct tgatctcatc
    34621 cagagtgcgg tatacagcct tgcttccagc atcgttgctg ccggtagctg ctgcgccgtt
    34681 atcgctgctg ccgcatgcgg tcagccccag tgccaatgct cctgttaatg ccagtgccaa
    34741 aacgctcttc cactcaaact tcttcataat ccttttcctc ctcttcgatg tctcatttaa
    34801 gtatcgaatc aacttatggg taggattata tctcctattt catactatgt caataggaaa
    34861 acagtaattt atttttactt tttgcgaaaa aataaaatca atgctataat actattgtta
    34921 cttttgattg taaacagacc ttgtaaccgg aattccgtaa gagataataa aggagaacaa
    34981 aagaataata tatgaaaaaa ccgatttcac gtttctatct gctgttggtt ctcgttccgt
    35041 tactgttact ggtgagcatg ggcggctatt actttctgga agaaactgct gcaggcagcg
    35101 atgttgttgt cgttccacag acccctgcac cgccttctgc cacaccggtt cccgtcccta
    35161 ctccggagcc gacctccact cccttcccgg agcatgacat tacgctcatg gctgtcgggg
    35221 acaacctgat gcatctggga atcgtggcca gtggaaaaca ggaggacgga acttatgatt
    35281 tctccatgct gtttcagggg atttcaccct tcttaaacgc ggcagacatc cggatcgtca
    35341 atcaggaaac tgtcatgggc ggcaattccc ggggattttc cggttttccc tattttaatt
    35401 ctcctaccga agttggggat gcgattgcgg atgccggatt caacgtggtc ctgcaggcat
    35461 ccaatcatac cgcggatcag aagctggacg gcctgttgta ctgtgcggat ttctggaaaa
    35521 acaagcaccc ggaggtgttg gtaaccggta ttcacgaaga cgcttcacag gcagatgaca
    35581 ttccgttact taccatcgat gatgtcactt tcgccatctt aaattatacc tacggtgcca
    35641 ataccgagac actcccctta gatctgctgg ggcatctgaa tcttctgtgt gccgtaaacg
    35701 agaaaaacgg acagatggac ttcaccacgc tgaacccgca ggtcttagag gatatctccc
    35761 gggcaaaaga actggcagac atcgtcatcg tctgtcctca ctgggggact gagtattcct
    35821 ccaaaatctc aagctatcag gaaaaatggg cactggagat gacagaagcc ggtgccgatg
    35881 tcattatcgg aacccatccc catgtggtag agcctgtgga atggatcacc gcagaaaacg
    35941 gcaatgagtc tctgtgctat tattcgctgg gcaattatgt gtccacccag aaaaatgctc
    36001 tcagcatgtt agagggtatg gcctggctga cttttcacgt tgcagaggac ggtgtctcca
    36061 tcgagcggaa gaccaccggt gtgatcccgc tggtctgtca gtacacctcc ggaccggtac
    36121 ggtttgaaaa tgtctatctg ctggaagaat ataccgaaga gctggcggcc tcccacggca
    36181 tccgcaatta tgccggtgtg gtccttcatc tggaagacct gcaaaaatgg agcgacgaga
    36241 tcctcggtga tttcgtactc accaaagatc aggcgctcca taatttcaaa accgattcta
    36301 ttaatcaata acgtttcgca taccgatgat gggactgtag tccacagcac tgataatgac
    36361 acctttccgt gaattggcgg aatggatgat ctgtccgtca ccgatataca gtcctacatg
    36421 ggtacagctc ttccctccat tggaactata acatacaata tctcctggct gaagttcttc
    36481 agaactgata ctgcgtccgg caccggaata ctgtcctccc ggtgtacggc tgatagaata
    36541 tccgaactcc gcatagataa agcaggtaaa tcccgagcag tccgttcctc ccgacaggtt
    36601 ggaaccaccg tgtacatacc ggttacccag atactgcatg gcatactcaa tgattccctg
    36661 tctgagctcc gcatttgagg tataggttgt agtgggaagt gtgatgttcg tgatcacctc
    36721 cggggcatcc tgctcggcag tctgttctct tgcctgcaat gccttctgtg cggcaatttc
    36781 cgcctgttcc tcttcaatgg tcttagcata gatgaattct tcggaaatcg tcacgtattc
    36841 cgcagagaca taaccttcct tgtcttctgt atactgtacc ttcagccatt ccccgtcatt
    36901 ctcgacaatc tcagccttct ctccgttatc aatatagccg atccgctttg catccgtagc
    36961 cggttcctgt ctcacattca gacggtctgc gataaccaca gcatatctgg tcacataatt
    37021 gtctacaacc tcagctgctg catctccgta aataaaaaac tctgccttga tatagccctc
    37081 cacattaccg gagatgatcc ggaaccagtc ctgatcctct cctgccacat ccaggatctg
    37141 tgccaccgct ccgtcataga ttttgcccac tacggtactg tccgtgttcg gttctgtccg
    37201 gacattgacg taattcgtca cattggcgat cgctaacgat gcatactcat ctgcgttcgt
    37261 atcctctacg gaagcttcgt ctaccggaac atcctccgga tccgacaccc cttccagtgc
    37321 tctgggatct tccgtctcgc caccctgtac cgccagcaga tcctctatgg taatctgtcc
    37381 ggtagccggg atcggctcga caccgatctg atgtattcct gccgcatgta ctttctgtac
    37441 cgaggtttca ctctgtattt ttagttctcc gtttcctgct gccaccacca tactgactcc
    37501 ggtgagagcg accgccagtg ccatgagcat tctttttttc attataatcc ctgttacttc
    37561 ctacaacttc agttttctga tgacgttcgg aattataaca catatgacaa caccagtcca
    37621 caatttttcc taaatatata taaaacgatt ctgccacata agaatacaaa ataaataaca
    37681 aaaaaaattt ttcatatctc ttatttctga agtccgggat caaaaaacgc ttacccccag
    37741 cccaacggca cccggaccgg tatgcgttgc aatgcttgca gacagaggat tataatacac
    37801 atccgctgtc ggaaatgctt cggaaatcat ttccatccat tcatttttac attccggact
    37861 taatcctgct cctgctgttc caatgtgcag ctgttcctca ggtacatcga aaaaacgatt
    37921 cccgatatca gctttgatgg cctccagcat tttctgcttg catttcttca tattgcctcg
    37981 gacttttgcg aaggaatcca atttctcccc ctgaattgtc agcaccggtt ttatacttaa
    38041 tacactgcca accattgcag cagccggtgt aattctccca ccctttttca aatattccag
    38101 ggtatttact gacacatata tactggaccg gagagcctct gcttccaaaa ataacttgat
    38161 ttcctctgca ctcttatctt ccattgccat tgcttttgct gtcagcaccg actgccttag
    38221 cgtaactgaa atcctatgat tatctgcaac gacaactctt ccctcatatt cctctgccat
    38281 tgccattgca gcgtgacagg agttactaag tccactggac atcggaatat aaacaacctg
    38341 atcatattca ttcaggagct catcccaggt atccagcaga tcccctaaag ccggctgtga
    38401 ggtagttact ctcttcccgg atgtaagggc atccagaaac tcttcttctg taagatttgt
    38461 tccctcataa tatgtctcat cctctataat taccggcatg ggcatcacaa atatgcccag
    38521 tttttccgct tcctctgcat aaataccgct atttgtatct gttaatattg cagttttcat
    38581 gttctttcta tctccctcat acaaaagtaa tcaaaaatag tgataaggca aatgagaaca
    38641 ggcatacaac gattccgcct attaattcct ttgccgtatg tcgcttaaga taaatggaag
    38701 accaactaat caacgcagcg accaataaac aaggaatgaa aactttccat ccaaaaaagt
    38761 agatcatcag caccagcggt cctgtaaagc tgcatgcatg cccacttgcc cgataatgaa
    38821 atgctttatt acatattgtt aaaataagaa cagaaaagaa atatgtcgag caaatgagca
    38881 gcagtgcgct ttctacatct gaaagaagag cccataaaaa tgccacacta tagccaataa
    38941 gattggtaac aaacgccagc tttctctgcc cttctcttcc ctgcttcctg aatcccggca
    39001 cgattccatt caggatgtat gccaggatcg gaacaaagcc aagaagcact atggacacaa
    39061 ctatttccaa agtatttctg aatataccct ctttgttaat ggctaaaata ataataagta
    39121 cagaaaccat aaccggtgga acagataatg ctcttattat cttcgcaatg cgttccttgc
    39181 tctccatgca cgatacctcc tgcacaactg tattgatgtt acaaattgta acatcaatct
    39241 gtgccttttt caatcagaaa gcttccttta tcacttattt ttataatttt catatgactt
    39301 aggacttacc ttagtctctg accacaattt atcagcacaa ggtttccagt ttcttgcata
    39361 ctcctcacac tttgtgcata aatgctctgc tgtttccgga gattgtaaat ccgtggattt
    39421 ggcacctgtt tcccttacaa ttctttgcag gatctccgga ttctcaagca tagggcaggg
    39481 tctcaaatgg ttctcattaa atggctggtt gtcacggtat gccataaata aaggttgttt
    39541 taatatttca agtaagctgt gtgttcttat atttgcccct gaatagtgga taaatacaca
    39601 gggctctgca tctccgtttg cattgatgtg aaaataattt cgtccaccgg caatacatcc
    39661 gccgacaaac tctccatcat tctggaaatc catcgtaaac aagccttcac cattttccag
    39721 ccgacggatt tcccggattc tatccttaat atagcttctc tgttcgggtg taggaagcag
    39781 ttctaccgac gcttcatttc caacaggcat atagtggaaa tacatcgcat accggcagcc
    39841 tttatctacc atcagcttca caaaatcttt gcttgttacc gactcgcagt tctttgaagt
    39901 ataacagatg gatgtaccaa aaatcagacc gttttctttc agcagatcca ttgcatgcat
    39961 aacctttgca tatacaccgg ttcctctgcg cagatcgttt acctccggtg agccctccag
    40021 actgattgcg agcatcaggt ttcctactct cttcatttcc ccacaaaaag cttcgtctac
    40081 cagtgtacca ttggtaaatg caaggaactg gcattcatga tgctcctcac acaacttgat
    40141 aatatcgttt tttctcacaa gaggttctcc gccggtaaac atataaaaat aaacgcctaa
    40201 ttcttttccc tgttttacaa cacctgataa ttcatcataa gagagattca gtttgttgcc
    40261 atattcagca gcccagcagc cggtacaatg aagattacat gcactggtag gatccattaa
    40321 tatcagccag gggatattgc aattatgcac agttctcatc ttacgaattg ttttggtgcc
    40381 ataaagcata gcctcaaaac caaaattgag aatagtcgtt tttattacat taggatcaag
    40441 ttcatccagt aaatgattta aataatggtt taaagcacag tcctcattac agaccatttc
    40501 ccttattttg tcataatcaa cgtcaagctt ctctcctgac atgtacttct ccataagatc
    40561 tataagcttt accatttctt tttctctgtc tttaccaata ttcttaagaa gtacatcaat
    40621 aatttttgaa gcggctactc ttgccgcagt atgtcctaaa ttgctcattt ttcattcctc
    40681 cgttagtaaa tttctctatc agcattcata gcatctgttt atgtatttct gatcattctg
    40741 actgtatttg ttacaactac cagattcatt attccatcaa gactgatacc aagtcctact
    40801 accctgacga tcagatctgc agcttccatc ggtgaaaaca aaagaaatcc cccgacaatc
    40861 cctgctgcca atgctgttat caggatccat ttccatcgct cgattccaaa tttcctggcc
    40921 tcgaaggatg tctgtatctt caatgcggca tctaccagca taaacatccc catgcagaaa
    40981 gacagaaatc ctacaatatg atttgtccgg aaaatcatca gtgctcccat tatcaccgat
    41041 acaattccca gtccaaagtc aaattgaaac gctaattgga acagatcctt agtaaaataa
    41101 ctggatattt ttgcaagacc atatatcagg aaaaaaactc cactgacctt acacagcaca
    41161 tccagtccta gtcccggcca tatcaaaagt aatataccac caacaataca gaatattgat
    41221 attgccacat acacattttt tgctcgtttt aaacttctca ctattttttc ctccttccat
    41281 tcatcgatca taatcccaat atcactttct tcaaacagac aaaaaaaaga cagtgtcctg
    41341 tttttgggca ctgtcaaaaa tgtgtctgat ctttactttt gtcttcttct atttgtgcaa
    41401 ttaatgcttt aatcgttgcc tgataagaaa tggacatttt ataaatgagt ttctcttgtt
    41461 gttcctgcat tcccctttgc atccagcgga gaatatttcc gatcatggaa ttactgtaaa
    41521 aatcaacaat aaactgaata tctgcttccg aaagccttga tccttccact tctttctgca
    41581 catattttct cacaaaatcc tctgtaatat catataaata tttcaggatc aaatctctgt
    41641 ttttagaatt atagagatga tatacagctt ttttatatgc gagaacaaga tgatatttat
    41701 ttaaatactc ctcataaaaa gaatcatttt cttgtttttc ttttaaagtt ttctgtgtct
    41761 ctgtccgaag taattcctca agtacctgat agatatcact gtaataatag taaaaggtat
    41821 ttctgttgat ttcacatatt tcacaaatat cctttacggt gatcttatca atggactttt
    41881 cctgaagcaa gttaagcatg cagtgcataa ttgcctgttg tgtaaatctg gacatcatac
    41941 ctccactctg tctggcataa ttaatcaatc ctcttctgtc cccaaaagaa tattgacacg
    42001 gttccgggtc ctgtatggct gccgatcgta gttccgatat tgttaatttc cactttacca
    42061 ttcagttttg gaaatctctc ttcgatctgt ctggcaacct ctttcgcatc ttccatacag
    42121 gcagactgac ataaataaca ttttccggaa taattcacac catcatcagc aagctcctcc
    42181 attttatcaa cagcagcctt gaacaccttt ttcttcgttc tgattttata gcgcggagtc
    42241 agtcttccct gatcatccat attcaaaagt gggcagatac taagcacctg gccaatggct
    42301 ccggctgttt ttgagattct tccgcctttg atataaaatg atagatctgt tgagaaaaac
    42361 cagtgatgca ttcgtaattt attctcctct atccaatgat ataattcgtc aatcgtcatt
    42421 ccctgatccc gtaatcccgc catggtatcc atgatcaatc catatccgga ggatgcgccc
    42481 agagaatcca cgatatatat tttccggtcc ggaaactctt cctctaacat tcctttcgca
    42541 atattagcag aattgatcac tccgctgatt cccgatgaca ggcacacatg aaggatatcc
    42601 aatccctgct ccaaaaaaga cctgaaatat tctgcaaact catctgcatt aatttgtgat
    42661 gtttttgttt ctgcaccggc tgccattctt gcataaaaat catcaaacga tatactctgc
    42721 cccagatcat ccagataagg tactccatct aattcataat gaaagcaaat atattttaca
    42781 ttaattgctt caaaatgttc tttggaaaga tctgcagtcg aacaacaact cagtacataa
    42841 ttattcattt ctattttctc ccgaccagct attgcatata tttacattac acaaagaatt
    42901 tgctgttaca tattgtaaca gcaaattctt cagattgcaa ttgcaatttt actactcata
    42961 gacgcctgtc tcaaactact taaaatactg catcaactct tccagatcag tggatacctt
    43021 tttaattgtt cctgtatcgt ccgaaatttt gttaactgct tctgcgatat cagatacgtt
    43081 tgccgttact tcctcgttgc tggctgcatt ctcttcactg atcgcactca gttctgttat
    43141 actatttata atagtctgtt tcaattcctc cagattcttc gttttttccg caataattcc
    43201 cgctgtcgta acattagcct caataatctg gctcagagca tcaaaaccat tcttggcacc
    43261 tccgatgtct gcctgctctt tttctgccag tgcacgcatc cgttcagtta actctacaga
    43321 tttattagat ttttcgagaa tatcatcagc aattttcttg atcgtatccg cactctggct
    43381 gctctgttcg gcgagctgtt tgatctctgt agcgaccaca gcgaagcctt tgcctgcctg
    43441 tcctgccctc gctgcttcaa tggaagcatt caatgacaat aaattcgtct gtgcggtaat
    43501 atcagaaatc agttccactg ctttactaat ggatgcaatg gcctcattgg ttgcctttac
    43561 ctgtacgatc atctcagtaa taatgtccga agaggtatgc gagctttcca gcaccagatt
    43621 catggattct gaagcattac gattggcacg atccatttta ttggaattct cattcaatgt
    43681 tactgtctcc gaatggatcg cattaacatt gtcccccatc tccaacattc tggaattaac
    43741 agtctgtaca ttctctgcca gtgatgtagc ggttgcagcc agctcgtcca cggcctgtct
    43801 gatctggttg gttcccttac tgcttgcaga tgccaatgta tttaataatt ccatgctgct
    43861 atccaattgt gcagcatgtc catccacctc ggtgacaata ctgttcaatt tatccctcat
    43921 gctgacagat gcccggatca gagtggtcac ttcacggatc acagatttca cttcaagatt
    43981 gcctgacaga ttaccattgg caatttggtc aatatattct gcggtcgtca gtaatggttt
    44041 tcgtattttg aatgacagat aaaccatgac agcaataaaa agaacattca gacatactga
    44101 gcggagaacc agacttctta acatgtcgga tattgcatcc tgaactatgc tctcccgttc
    44161 tccggcaaaa gccattccca gaatctctcc gtcatcggaa cataccggca tataatagac
    44221 ataatattct tctccctgaa tctctactcc atcagtttca taatcgttac cggctctgac
    44281 cgtctcccat atctgtggat cagccttggt tccttctgcc ctgttcccat ccttgtccac
    44341 aatggaagta ataaatctct catcctccat gaaaagcgtc tgttcaatat gctcatcctg
    44401 caaagaatct ataaactgat aactgatatc atccttttcc agaatatcct cacggatatc
    44461 ccatgtgaaa tactgctcca ctgatgtagc acatgctttc agccgcagat aggtactgtc
    44521 gaccagttca tctttcattt tgcccgatgc annnnnnnnn nnnnnnnnnn nnnnnnnnnn
    44581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    44641 nnnnnnnnnn ngtctgaaat ttttaaaaat aaagaatgct tacgccatgt aacatcgtgg
    44701 tatttctcca gttctccgtt cccaacgatc cggtgtgcca gatcagtagt ccggtgtttt
    44761 cggcattttc tcttctgcct acttctgccg taagctccac tccccgggca tagccccttt
    44821 cctgctgtac caccgtaggt tccgtaaccg tccatgcttt tgccgataat attccgttct
    44881 cataccgcac ccaaattctg catcccgtta caaagcagtt tgtcacgacc tcgcgggaaa
    44941 tgcactgcgc ctgtccacgc acatactgta tcagatacat ggataccgca tcggaatggg
    45001 ccgccgtacg gatgatccga agtccataac cgtccagagt gtccgtgtcc gtcttgatgc
    45061 agacatccag atactgtccg gcgctgccaa aaccctgtcc ggcagacttt gccggatcga
    45121 cttccaacaa gacttccata ttccgtttgg tttccgttcc catctccggt gccgtggtcg
    45181 gcgtatagcg gatcctggcc ccctgcattc cctgatagag cccttcgccc ttactgccgt
    45241 cccctgttgc accgtagtgc caggagacct gcgtcttttc ccagtcccac ttttcaaaat
    45301 cacaggtatc cgccggacgc ttcgcatcaa aattccatac acctttttcg tttccggtat
    45361 ggctgtaaag aggaaggtta tgaaaatctg tccagatcct gtctgtttcc acatcttccg
    45421 gcgaaatagc tttttcataa aagcactgca ctgcttcccc gtattcgctg cgattcgttc
    45481 tgggtcttat ctgtgcgaaa atataatatc ccacatcatc tccggtgagg gcataaactt
    45541 tctccggctg atccggttgg gagatcgccg tcacgatttt gtcctctccc cgaatgtttc
    45601 tgcttcgata ccagataata tccgagcagt cattctccgc ttccgtaaaa tcataagaca
    45661 gccgcagcat tttgccttca agacagatca ccggattgcc cgtaagcctt ggtgccgcca
    45721 ctttccgggg atgaattctg acatacgcac cggtctgcaa tccctctttg gtctgcgccg
    45781 tgagtactac ttccgtctca tggtctatat tactgtcttt cagttgcagc agacagctcc
    45841 cctccgacac acgctgtatt tctatggagt ctgcactgtc tccttctatt ttaaaatgta
    45901 tctcacactt cctctcacgc ccatcaaagg tcaggcattt tccctttata tttatggaag
    45961 atccgcctgc ttccagttca aaagcctctg actccagcag aagctgtgta ggaatatctg
    46021 tttttccggc aaagcggatc acttctccgt tccccaacgg gtcccagtcg tctttcccgc
    46081 ccagcagatt atagacattg tagatcgttt ctccatcctt acggatatag taggcctctg
    46141 ccagcatctt ttcgtcaatg tccaccgtat gggaatctgc aacctccgga ggcgtaaacc
    46201 ggctttccgg atatgtcaca ttcgcctgat agcacttcaa tgctacggaa ggatatttcg
    46261 tccacaagac cgcaactttg gtgtccggca gacctgcgaa gctgctgtcg atcagtgcga
    46321 tctgtcctcc gacttttgta aaatactgtg cgtgagtctc cccatcctgt ggatatttac
    46381 aatgaaatgt acagtttaag aataccgctc ccgtaccgga agcctgatag atcggcattc
    46441 cgctgtaaaa atcaaactca cagtccaggt acactgcatt tccattcaga gcatcgtccg
    46501 tctgttcaaa acgacaatgt tcatacaggc ttctgcccgc gccacatacg ggatacaggt
    46561 tcagacgact gacaaaccgg caattcttcg catggaactt atcggcattc gtatccgcca
    46621 gctgtgcctg cgtaatggct tctgtcctct ttttcacaga ctgcgcagga tccaatgcat
    46681 aatccagatc cacacagcaa taattcccca gagtcaggtt ttccatctcc aattgttccc
    46741 cggaaaatgg aacagagtat aattcccctt cgctccatgg tcgttccctc ggttggcggc
    46801 aatcaccaca tcctccgggt tctccgacag ccccaccaat ttcaaatttg cgcagtttac
    46861 cttgcatccg taagggtaaa gatccttcgg atcctccctt acgatcaccg cctccgactg
    46921 cggatcctcc agccaataga tgccgggagc aatgtagacc gtcaccgggt gttccacagt
    46981 tgctgcgttt agtgctccat ccgacaggac ttctctgatg ttattgtaag tgtatggatt
    47041 tttctgtact tcctcgttcg caagcctgct gtccagcagg agatgacagg aatcggggtc
    47101 aggttttatc ataaatgttt ttcccttcct cagaataaaa taagtgaccg attacttttt
    47161 actatagtaa acgaccactt atttatcaag agattaagtt attaatatcc taccatagaa
    47221 gtaaaaatta tggtgggaag tcatgaagaa ttgtatcatg ctatggaaaa atgacgctgt
    47281 tgatcttgcc atcaacaacc agagaagagc tttttcggat gcgtataata atgaagtgct
    47341 ggcagaaagt aaaatttata ttgaactttc ctccaaaaat ccactcagta aattaagttc
    47401 cctggaaacc gaagatttaa aaaatacccc ctgcattctt gtcatcaacc agaccggaca
    47461 gcaggatacc gcttgccttt ttcacacttc taataaacca ctgctgccag aatgatatat
    47521 accagaatcg acaccataca gtacatagaa ttggttgttg atacataggc actgccctct
    47581 tcctttttca gaaaagtctg catgctgaaa gtggccggcg ccgacatata ggaaatgatg
    47641 gcaagattca acagcatgtt atctcctatc cacaaatgca ctgcccacag cactccaatt
    47701 gccataagtg tctgtagcag aactctcatc aggattgtct tcaggcatgg ccggatcagt
    47761 tcctttgtca gttccatgct atatcccaca acgatcagga tgattgctgt cacggatgtg
    47821 gtaaggatcc cttccaccgc cagatatgca ccgccgaagg gactgtcgat caggcaaatc
    47881 accaccttgg acagtcctgc caggattcca agcacacttg caataaatgc cggtgtgtgg
    47941 aatgcactca tacatagttt ctttgcattg atcttctctc cgttttccac ctgtcccagc
    48001 atccccatat agatgctgaa tccaaacaaa agtcctgcaa tatccagtac tgcgatctgt
    48061 gagagatttt cctgcccgca caggctggta tacaaaggat acgccatgag gccaccctca
    48121 tatacactga ccataaacgg caggtacttg cggtattctc cgttcataag cggcttcaac
    48181 agaaatccta ctccgaaaga taccaccagc ataacgaaca taatccccac cagtttccag
    48241 gtctccacac tgtaatccgc tgtggccagt gcatgaaaga ttgccaccgg aagcatgata
    48301 ttggtttcca gcagtttcat attatctatt ccgcttttgg taagtaactt taattttctg
    48361 catgccatac cgagaaccag catgacaagt accggcaata taatttctaa tattctcatt
    48421 tgttatccct ttccgaggta tcctatactt ttttatttat tatacttcga ttttcctatc
    48481 ctgtatatta tgtatcccgg aagttttgta gaaatgtttt agaattgatg cagaaatgtt
    48541 aatgccagaa ttcggtctcc atttcagata tgcaaaaagg cactgtatct caattttttt
    48601 gaaatacaat gcctttttta caacacaatc ttacccaaag aaattatact tgttagcctc
    48661 aaaagcttcc atataggttg ccccattgga accataccgg ttggatgcgc tgaccacatt
    48721 ttcggaagca ttcttattga tgtgctcacc gagaaatcct attttggggg cgatgccatt
    48781 ctctcctccc agtaccttct gtaaggtatc cgcatccgca ttccggaata ctttctcgtc
    48841 cacgatcagg cttccgtcct ttgcctgact gattcctata ctttccagtg attctttact
    48901 gcccgctgga atgtttttta actgttgctg gtaaaattca ttcatgattc cgccggtggt
    48961 cttaagctgc ttcatggtag cattgtacgc ttccaccatt ttgttcacat cactcaggat
    49021 gtccgtggtg tctccgctct cttttgcctt gtcgtagagg gaatccttat cgctgcctgc
    49081 aagctttgac gcagattggc tcaacttctc cgacagatcc tccagctttg cataattatt
    49141 tttcctcaga atgtccgtgg caccggtact tgtggtgttc atgctcttcc cgatggttcc
    49201 cagcaggttc tgggatgact tgttattgat aatatccaag agcgacgttc tctgaatggg
    49261 aatcccgctt tttgatgcgg aatttgccag catttgggtt gtgatcctca ttttgcgacc
    49321 tcctttgatc tgcaattttt tctctctgtc ttctatatcg ccgcttttct ttcaccctta
    49381 atatgtcttg taacgaaccg atcttttcat gtaacgaaac atcaaaaaat cagcaggccc
    49441 atcacccgct gattcctttc tgaagcatca ccgcttacac ttttctcaga tacttcatgt
    49501 atgcggtgac aaagtcattg tatttctgtt ttgctaaaaa tacgctctca ccgtttttca
    49561 attctatgtt gctgttgtca taccgggcca cctcctgcag attcacaaga aatcctcgat
    49621 gggagcggaa aaagcgatct cccagcttct gctccagcac ttccattttt tcataaaaac
    49681 agtagggctc cttctttgac ttggtgtaca gcataatttt tctggcctgg ttctcggcat
    49741 agaggatatc atccacagga atcctgacat agttgccgcc tacttttata accagcggtt
    49801 caacattctt tttactccgg atctggcaga ttgccctgtc catgacttct gtgaacttct
    49861 gctcatcgat aggcttcaaa agataatgaa acgcccccac atcatagcct tcaaacacat
    49921 attcacgtaa ggccgtcacg aagataatca gtgcatccga cctttcccgg atctttctgg
    49981 ccgtctccat accgtccggt tccgccgcat tctgctcccg gttcagggaa atatctaaca
    50041 ggatcaggtc aaacgccgtc ggatccgcca acagttctcc gccgtcggca aacagtcgga
    50101 tttccccctc cgtacatttc tttaccagcc ttttcaggta ttctcttatg ggctgttcat
    50161 cgtcacatat tgctattttc agcattcgtc ttctactccc ccggatctta ttccattcaa
    50221 acatttcctt ttttatatcg ccacttttcc tttgaaattc aatcctatgt aacgaaatgc
    50281 gatttttgtg taacgaaaaa atcagcgggt atatagcatc ccaaaagctc tctcttgcga
    50341 gagagcttgg tctgcacctg taaagtcaag gttgttttgg tgtaaatagc ataaaaacga
    50401 caaattccat acaaaaaatt tcccaaatca gaaatttttg tacggaattt acttattttg
    50461 cttatatagc gaaaatggag acttttcaaa cggtaaaatt ccacattttt gtattttttt
    50521 cgtccaaaac ggcgatttaa atacaaacaa gatttccact gactgtcccg atgcaacgct
    50581 cacaaagaga attactatca ctctgcgaac agttccttct tccgttcaat catctcatcg
    50641 attttctcga tataaagccc tacatccttg acattatcgc atctttcgat ccgtccgtcg
    50701 gggaagacac gcttggtgat gctaccggca cccagagcca cgatggtctg tttctcctcc
    50761 atgatgagga tgttgtacag gccgtatttg ccgggacggg cgtagccggt gttctcgaaa
    50821 ttaccggaca tattcttctg tcggtagaga taatacggct tcattccaag cttttcagct
    50881 cccgctgccg cgatcttcat ggtctcgtct gtgttgtgga gcatggagac gccgttctcc
    50941 tggatccact ggttcagacg ggatgcacgt ttgatggcca gggaatgaac ggtcaggctg
    51001 tccggggcca gctccacgat cttttcgatg gtgcgctgta catccgcctc cagttctccc
    51061 ggcagtccca ggatcagatc catattgata ttgtcgaagc cgacctctct cgccagctta
    51121 tataccgtaa gcacttgttc cacgctggct tttctgccga taatatccag ggtctcctgc
    51181 ttcatggtct ggggattcac ggagatgcgg ctgacccggt gacggtacag tacctccagc
    51241 ttctctctgg tgatgctgtc ggcccggccg gcctccacag tgaattcctt cacggtggaa
    51301 aaatcaaact tggactttaa tgccgtgatc aagcggtcca gctgctccgg ttccagcgtg
    51361 gtaggcgtac caccgccgat atagaccgta tccagtacct tgtccttaaa attctccgcc
    51421 acataatcca tctctttgat cacagcgtcc acataagcgt ccacctgcct gcggaatgca
    51481 gcgatcggat aagaggtaaa ggaacaatag agacaggttg tagggcagaa cggaatcccg
    51541 atgtacaggc tgtagccgcc ctcataatga atctctttta acagatcccg ctctctcttc
    51601 gcaatatcga ttcccaagag tgccttctct tcgctgacgt aatggctctg ctccatgaag
    51661 tccaggatct ctgcatcgct tctgccctca tccagcatac cgtaggcaat cttcgtggga
    51721 cggattcccg tgagatttcc ccagggaagt tcctgccctg tcaccttact taaagtccgg
    51781 tacaggaaac gtttcaaacc atttttataa ccatctgcga ttgtctcaga tgccgcatac
    51841 cacagaaaat cctcttctcc cacacggatc tttgctccgg tctcgcccag ttcgatttcc
    51901 agactgacct gcggttccag ctctgccagc ttttccggct ttgtctccgg ggtgatgact
    51961 gtgacctgct cctcggcata aaaggccttc accagagaat gcacatcgta ttcataattg
    52021 ggatgggata atttcactac tttcacgtta ctacctgtac ctttcctttc gcccgcctcc
    52081 gcaaaacgca ggcacataac tgttcagaac cccattcgca aaaccgctgc tacgcagctt
    52141 cccggaataa catgccggca ttcaacgaat atctgacgat atttgcatgg ctccgaaggt
    52201 tatacgcaga atggattata cttcttctca tgcccgatgg tagtgctgtc tccatgtccc
    52261 ggatagacta ccgtctcctc cggcagagta aacagcttct ccttcaccga acgcaccagt
    52321 gtactcatac tgccggtagg aaaatccgtt cttcccacgg attcctggaa cagggtatct
    52381 ccacagatca ggaagcctcc ctccgggaaa taatagctga caccgcctgc ggtatggccg
    52441 ggagtcgcca gaacctggat ccggatgccg tccagatcca gttcttcccc atccttcaga
    52501 tacacatccg cggtgaccgt taccgggcgt cccatatctt tcg
//
LOCUS       DAWDHD010000003        44995 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_72_length_44995_cov_13.804851, whole genome shotgun sequence.
ACCESSION   DAWDHD010000003 DAWDHD010000000
VERSION     DAWDHD010000003.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 44995)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 44995)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..44995
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72_length_44995_cov_13.804851"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    30118..30217
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT        10649 a        12177 c         9269 g        12800 t
ORIGIN      
        1 ctacggtaat gagcaatgca tctcgtaaac gcttcgcgtt tccttcgatg tcgggcattc
       61 gccctatcaa accgcaatct gacgtttccg cctgcaagca ggcgaatcgt catcttacgg
      121 tttgacattt ctcattacct acgcaggttg ttggcctgct gtagcatctc atcggatgct
      181 gtgatggctt tggagttgag ttcgtaggca cgctgtgcaa caatcatgtt gaccatctcg
      241 tctacgacct gtacattgga gctttccaga tatccctgga taatactgct cttttctaca
      301 ttattatttc ttgcttcgtt gatagcctgt ccgctggctg cggtctgctg atacaggctg
      361 tctgccagtc tttccagtcc gttcggatta ttgaactgga aaacacctat tgtgatgccg
      421 ataggctggg gattgttctt tgcatcggga taacacagac ttccgtcctt gctcaccgta
      481 acattgctca tcacataatt actgcccagt acaatgggtc tgccctgtgt atccagtacc
      541 ggaagtccat cagaagtagc cagcatattg ccgccgttgg ctgcaagagt aaacttcaga
      601 ttaccgtttc tggtgtagta ggtattgccg tccgctcctc ttactgcaaa gaaacccttg
      661 ccatcaattg caaatgcggt gtcgctgtcg gatgctgtca gacttccttc cttaaatacg
      721 gaactgatgg aagcatttct tacaccgaga cctacctgcg cactgctggg cttggtatct
      781 ccgtttgcgg aagttgtctt cgtctggatg ttctggtata acaggctctt gaattcattc
      841 acctgtgttt tgtaacctgt ggtgtttacg tttgccagat tgttggcaat cgtatccaca
      901 tttgtctgct gtgcgatcat gccggtcgct gctgtccata agcttctgac catatgtagt
      961 ctcctctcac aatgtccctt atgtcatcca tgacctgatt tttatcctga aaatattcta
     1021 taatcagtta tcggctgtcc cagccgaatt cattactaca gtttaccaat ctggttcgct
     1081 gctacatcca gagtgccatc aatggtctgg accaccttct ggttggattc atatgctctg
     1141 gtaatggaga tcagatttac catctcggat acaatctgta catttgccat ctccagatag
     1201 cctgacttca cctgtgcatc tgccgtagtc gttttggccc cttcaatggg ctgataatag
     1261 gtctctccat atttttccag gtaatcataa tcttcaaagt ccgtcacctg aattctggca
     1321 actgcctgat cgttctgata aatcgtaccg tcatcggtga tcttggaatc tagcagtgtg
     1381 ttcaggcgga tcctcctgtt ctgggtatcc agaacatagt caccctcttc cgttaccaga
     1441 tatccatcct tattcagtgt aaactgtcct gcacgggtgt atttggtgtc ggtctctcct
     1501 gccttattgg taaattcaat ggcaaaaaag ccatctccgg ctaatgccag atcataggta
     1561 ttatccgtaa tgcggaaaga tccctgcgta taatcggtat aattctctcc gatcttcact
     1621 ccgggatttt tgataccgat cttctggaca tttctcatgc ctaccgactg gtccttgatc
     1681 ttgaccgtca gcacatcatt aaaggactgg ctggtggatc cttccttttt atatccgacg
     1741 gtagatgcat tggccagatt gttggtcatg atatccattc tgttctgttc gtttaccata
     1801 ccggtgtaag cggtatacaa gcctttaacc attcatatcc tccatttgcg gctgcgatcc
     1861 ttcgacaaat gtacggcatc ctgccttagt ctatcttttc cggttccccg gagctgtctg
     1921 tgttactctc tgtagcctgc caggaactct acgtatttac cggttccaat ggccactgct
     1981 gtcatgggat cctctgctgt catggtgttg atacctgtct tggcagagat cagatcttcc
     2041 agaccgcgca gcaggcttcc acctccggtc agtacgattc ctctgtctgc aatatcggct
     2101 gccagctcag ggggagtctt ctccagtacg ctgtgaatgg tctccacgat ctgtgcggta
     2161 gtctcgcgca atgcctcctc ggtctcctcg gaagacacct taacagtctt gggcagaccg
     2221 gtcacgaggt tacgtcctct gacatccata taatccactt ccgctctctt gtagcaggaa
     2281 ccgatcttga tcttcagatc ctctgcggtt ctctcaccga tgagcagatt atgtttttta
     2341 cgcatataac gcacaatggc ttcatcaaaa tcatcgcctg cgatcttaat ggaagcactg
     2401 actacagtac ctcccagaga tatgactgcg atatcggaag taccgccgcc gatatctacg
     2461 atcatattac cgcaaggctt ggaaatatca atacccgcac cgatagcagc tgcgatgggt
     2521 tcctcaataa tggaaacctc tctggcacct gcctggtagg tagcatcttc tactgccttc
     2581 ttctctacct ctgtaacgcc gctgggaaca cagacagcga tacgaggctt acggaaagtt
     2641 ctcttcccca gcgctttctg gataaagtat ttcatcattt tctcggttac ggtataatca
     2701 gagataacgc cctgacgtaa cggtcttacg gctacgatgt tgccgggagt tcttcccagc
     2761 atcagtcttg catcctcacc gatggcttta atcttatttg tatctctatc aaaagctaca
     2821 acagagggct cctttaatac aacgcccttt cctctgatgt ataccagaat gctggcggta
     2881 cctaaatcaa ttccgatatc tgtgcactgc atctttttta ttaccccttt ccgatggtat
     2941 ctatctataa gtcttgactc tacacgctat tttagtcata tacatcatat tataactgaa
     3001 ttttttccat tttcatagag ggtaattctt aaaatttgat aactattcag aatcagggag
     3061 ccatgaaaga gcgttttgcg aatgggggtc tgaatagtta tcgtagtttc ttgtccttct
     3121 ccagcatctt tttcacatag taaccggtgt aggatttttt caccttgacg atctcttccg
     3181 gggttccctt ggcgatcact gtaccaccgc ggtctccgcc ttccggtccc atatcgatga
     3241 tataatccgc ggtcttgatc acatccagat tatgctcgat gacaaccacc gtgttgccgc
     3301 cgtccgccag tctgtgcagg atctccacca gcttatggac atcctcaaaa tgcagtcctg
     3361 tggtaggctc atccaggata tagatggtcc tgccggtact tcttctggaa agctccgtgg
     3421 ccagcttgat acgctgtgcc tcaccaccgg acagggtagt ggaaggctgt cccagcttta
     3481 cataggaaag tcccacatca tacagggtct cgatctttct gcggataaac ggtacatttt
     3541 cgaagaaagg caatgcttct tccaccgtca tatccaacac atcaaaaata ctcttgcctt
     3601 tgtattttac atccagtgtc tcacggttgt atctcttacc gccgcagacc tcacagggca
     3661 catagacatc cggcaggaaa tgcatctcga tcttgatgat accatcgcca ccgcaggctt
     3721 cacatcttcc gcccttgaca ttgaagctga atcgtccctt gctgtaaccc ttggcctttg
     3781 cgtccgccgt agaagcaaac agatcgcgga tcagatcaaa tactccggta taagtagcgg
     3841 ggttggatct gggagtccgt ccgatgggag actggtcaat gttgatgact ttgtccagct
     3901 gttccacacc ttcgataccc ttgtgtctgc cgggaatgca gcgggcacgg ttcaattctc
     3961 tcgccagatg cttgtagagg atctcattca ccagtgaact ttttccggaa ccggagacac
     4021 cggtgacaca ggtcatcaca cccagaggaa tttctacatc tacgtttttc aggttgtttt
     4081 cctgagctcc cagtactttg atccagccgg tgggagtcct tctggtctcc ggtacgggaa
     4141 tccgcttttt gccgctgagg tactgtcccg taatggattc cggaatctgc atgatctctt
     4201 ccgctgtgcc gcaggcaatg acctctccgc cgtgggaacc ggctcccgga ccaatgtcca
     4261 cgatataatc cgctgccagc atggtgtctt catcatgctc taccactacc agagtgttcc
     4321 ccagatcccg caggttcttc agagtattca gcagtttgtc attgtctctc tggtgcaggc
     4381 cgatgctggg ctcgtccaga atatagcata cacccaccag tccggaaccg atctgggtag
     4441 ccagacggat acgctgtgct tcaccgccgg acagggtagc cgtagctctg gccagagaca
     4501 aatattccag tcccacatcc accagaaatc ctactcttgc gcggatctcc ttcagcacac
     4561 gctcaccgat gaactgctgc tgtttcgtca gcgtcatggt atccaggaat ttctgcagat
     4621 cacggatgga catggatgtg atctcataaa tattcttgtc gcacacggta accgccagag
     4681 attccttctt cagtcgcatg cccttacatt ctttacaggg agtgatgcgc atgaaagtct
     4741 catattcctg tttgctactc tcggaagcgg tctcacgata gcggcgctcc acatttttga
     4801 tcaggccttc aaaggctact ggatacacac cactgccgcg ctggccggta taatgcacca
     4861 ggacctcttt accgttggtg ccgtggatca gaatgtcatg aatcttctgg ggataatcct
     4921 ggaagggagt atccagatca aaattgtatt ccttcgccag tgcccgcaga atagcattgg
     4981 taaagctgga cttgtccgca caggactgcc agcccatgac cgtgatggct ccctcattga
     5041 tgctgagtct cttgtccggg atcatcagat cctcgtcgaa ttccatctta tatcccaggc
     5101 cgaagcatac cgggcaggca ccgaaaggat tgttgaagga aaagcttctg ggctccactt
     5161 cgctgacact gataccacag tccggacagg aaaagctctg gctgaaagtc accggttcgc
     5221 cgccgatcac atccaccacc agcagcccct ctgccagttc caatacgttt tcaatagagt
     5281 ctgtcagacg gcgctgaata ccatccttta ccaccagacg atctaccacg atgtcgatat
     5341 tgtgcttgat atttttatcc agtttgatct cttccgtcag ttcatacatg ctgccgtcga
     5401 tccgtacccg gacataaccg cttctcttgg cgcgctccaa caccttggca tgctcgccct
     5461 tgcggcctct gaccacgggg gccagcaact ggatcttcgt gccttctccc atgttcatga
     5521 tctgatccac catctgatct acagtctgct tggcaatctc tctgccacac ttcgggcagt
     5581 ggggaatacc gattctcgca tacagcagac ggaaatagtc ataaatctct gttaccgtac
     5641 ctacggtaga acgagggtta cggttggtgg atttctggtc aatggagata gcgggggaca
     5701 gtccttcgat gctttccaca ttgggcttct ccatctggcc caggaactgt ctcgcatagg
     5761 aggacaggga ttccatgtag cgtctctgtc cctctgcata gatcgtgtcg aatgccagag
     5821 aggacttacc agaaccggac ataccggtca ataccaccag ctcgtttctg ggaatgtcca
     5881 cattaatgtt tttcaggtta tgttcattgg caccccggat cttaatgtat tcccggggac
     5941 gcatcttctt tgcttcttcc attgtcttgc ctttcttaaa taaaatatcg ttctccaatt
     6001 attcagagcc ccattcgcaa aatcgcatct ttgatgcttt acttttattc catatcattt
     6061 aattgtttct ttaattcaag cattttatct ctaagttctg ctgccgcttc gaaattcagg
     6121 tctgccgctg ctttctgcat cttcttctgg atctcgccga tcagcttctc cagttccttc
     6181 ttcgtcatgg attcgggatc cttctcaaag cgtacctctt ccttggcgat taccttggag
     6241 atgctgataa gatcacgcac cgcctttttg atagtctggg gggtgatacc atgttcttca
     6301 ttatatttca tctggatctc acgacgtctg ttggtctcat ccagtgcttc ccgcatggag
     6361 tccgtgatgg tgtccgcata catgatgaca tggccttccg cattacgggc cgcacggccg
     6421 atggtctgga tcagggaagt cgcacttcta aggaatcctt ccttatccgc atccagaatg
     6481 gccaccagag tgatctccgg aatatccaga ccctctcgca acaggttgat acctaccagt
     6541 acatcgaaca catccagacg catatcccgg atgatctggg cacgctccag cgtatcaatg
     6601 tcagaatgca gatatttcac ccgaataccg acttccttca tataatccgt aagatcctct
     6661 gccatccgct tggtcagtgt ggtgatcagt accttatgat gctgtgcgat ctccttgttg
     6721 acctctccga tcaggtcatc gatctgtccc tctaccgggc gcacctgtac ttccggatcc
     6781 agcagacctg tgggacgaat gatctgctct gtccgcagca gttcatgctc cttctcataa
     6841 tcggaaggag tggcggatac gaacatcatc tggtcgatat gcgcctcgaa ttcctcaaag
     6901 ttcaacggtc tattatccag tgccgagggc agacggaaac cgtagtccac cagtgtctgc
     6961 tttcgcgagc ggtccccggc atacataccg cggatctgtg ggatcgtcat atgggactcg
     7021 tccacgatga tcaggaaatc atccggaaaa aagtccaaca gcgtcatggg cggtgctccc
     7081 gcaggaagtc cgttcagatg tctggaatag ttctcgatac cggaacagaa accggtctcc
     7141 cggagcatct caatatcgaa attcgtccgt tccgcaatcc gctgggcctc gatcagcttg
     7201 tcttcgccct tgaaaaactg tacccgttcc ttcatctctt cttcgatatt gtcacaggct
     7261 gccttgatct tctcctgaga taccacataa tgggatgccg ggaagatcgc cacatgattc
     7321 agctctgcat gaattttccc tgtaagaggc tctacctctg cgatccggtc gatctcatcc
     7381 ccgaaaaatt cgatacggat gagataatcg ccaccctgtg ccggatatac ttccaccaca
     7441 tctcccctga cacggaaagt ggcacgctga atatccatat cgttgcggtt gtactgaata
     7501 tcgatcagtt cccgcaatac ctcgtcacga tccttcacca tgccgggacg cagggatacg
     7561 atcagatcct gatattcgtc ggggcttccc agaccataga tacaggacac gctggccact
     7621 accaccacat ccctgcgttc cgccagggat gctgtcgctg acagacgcag tttatcgatc
     7681 tcatcattga tggaagaatc cttggcaata taagtgtccg aagacggcac ataagcctcc
     7741 ggctgataat aatcgtaata ggagacaaaa tattccacgg cattttctgg gaaaaattcc
     7801 ttcatttccc cgtaaagctg ccccgccagt gtcttgttgt gggcaatgat cagcgtcggc
     7861 ttattcaatg cctggatcac attggccatg gtaaaggtct taccggaacc ggtaacaccc
     7921 agcaatgtct caaactgatt gccctgccgg aagccttcca ccagctccgc aatggcctgc
     7981 ggctggtctc cggtaggctg atattcggaa tgtaatttga attgtgaggg ttcgttcgat
     8041 ggattcatga tgcctctccg tgcaaatatg ttactggaat aacacagttc gtgaacgtat
     8101 tcggttctaa aatcatacag acatgatcct gttcccggat acgttcacgg ctgaactgtt
     8161 atctattttt tcacgttacg cacagattat agcagaattt ttttgaaaag tacagatgaa
     8221 ttcgaaaata tgttcggttt gctgtttcct ctccacatca cagcgcacag acgttttccg
     8281 gctttccgtt cagataagcg tacacgttgg aaaactcaat cttcgctctg cgtaccatgg
     8341 cttcctccga caggaatgcc acgtgcggtg tcaacaatgt atttttcgca tgaagcaacg
     8401 gttcctctgc gggaatgggc ggctcacagt caaatacatc caccgcagct cctgcgatcc
     8461 gtccttcgtt caaagcctgt gccaatgctt tgctgtccac gatcggtcct cttgcgcagt
     8521 taatgaaaat ggcggacttt ttcatcagag caatcttctc tgcactgatc atgcctctgg
     8581 tctccttatt attcgacgta tgtagggaaa taatatcaca ctctctcaac aggtcatcca
     8641 gcggtttgta tgcaattcct gctgccttca cttcttctct ttcgtgtctg gcataggcat
     8701 agacttcagc accgaatgcc tggaaaagct tcgctgtcat gaaaccgatc tgaccgcagc
     8761 cgatgattcc cacctttttg ctccggatct ctctgccaca cagtcctgcg ctggtaccgc
     8821 cgtttctcac cgcctgatcc gcctgctgca gataacgcaa aaaaagcatt cgtatcacta
     8881 caaatgcttc tttgcatgaa tgataatatt tttcgtttat ttcttgttgt ggcttatagt
     8941 atcacttaac tttttacttt tcaagtgctt tttgcacaat tttatatttt tcttcctgtg
     9001 catgatgcac attctctaca cgataaagtg ttccacattt tgctgaagtt tgtccgcagc
     9061 aattcttgca gcctccgctt tttccttgat ctcgttggct ctttctacga ttatccccgt
     9121 cttatccgca atatccgtag ttcccgaagc cccttcggta gctgccttgc tgacttcgtt
     9181 gattgcctcc atgacaccgc tgatagatgc cagcagttct tcggaggagg cagagaaatc
     9241 cgttaccagt ccatcgatct gaccggcatc attactgtaa gaatccgcca taccggaaaa
     9301 tgttgcaaag ctgtccacca catctgttcc gacaaatccc aacagcttct gtgttccttc
     9361 tgccagattg tttacagcat ccatcacatt gttggtcaca tcctggatat gagagacggc
     9421 tgccttggac tgttctgcca gcacacgaat ttcctccgcc accacagcaa atccttttcc
     9481 ggcttctccc gcacgtgctg cctcaatgga tgcattcagt gccaacagat tcgtctgtcc
     9541 cgtgatctgc ataatagatt ctgccagaac tccaatctgg ttgactactt taatttcctc
     9601 cagtgccttg gtcagtcctt cgttgatctc ttcatgaatg gctttggtac gctgatcatt
     9661 ttgtgtaata tccttcttga ttcctaccgc acgctcacgg atcgcatctg cttcggtggc
     9721 accatcctga gaacgtgtcg caatattctt cgctgcactc tcaatctcat gagacatagc
     9781 attgatctct tcagaagatg ccgctgtctc ttccattccg gctgccagtt cctctgtggt
     9841 agcagacaca tcctcgatct gttcatccag agcctgaata ctgttatcaa tattctgtac
     9901 catattgttg atctctgcag tctgctcttt gacttcaccg atcagtccac tcatctctgt
     9961 cctcatagac tccagagaga ctgccagccg tccgaaatca tcacggcgtt ttaaaagctt
    10021 atctcccata gattttgaga aatctccctg ttccatcacg ccgatggatg ccacacattt
    10081 tttgagcggc tggacgatgc tgataataat gaaaaccaga aggacgatca cgattgcacc
    10141 ttctataagt gtcgatgcaa tgaacatcgt cagtcttcct gtcacataac cggaaaattc
    10201 atcacctacc gtgacaatct tatcatcaat atagtcggtg taatttcctg tgccgatgac
    10261 ccactggaac ggttcaaacg ctttgctgta ggaacgtttg ggagaggatt ccgtctctcc
    10321 ttcctttggg aacacataat ctacataacc accatccggc tcctgtccca tgcggatgat
    10381 ttcctttacc atctggtacc cgttagcatc cttggtctcc atacggtttg taccttctgt
    10441 tgcacttccc agcagtacca cattggttcc gtcatactga tccacccaga aatatcccga
    10501 atctccgtag cgaagctggc ggatttcatc cgccgcaagt ttcctggcct ctgcttcggt
    10561 atagacacca gcctgatact gatcataaat ggtctggcag agcgagatca cattatctac
    10621 ctgctgcttg atctgttcat cgtaggcctc ccgctcatca gtctccatgg tgtctaatgc
    10681 tttggactgc aattctttca tgctttgtgc tgaaaagata gtacacaaca acagtgcagc
    10741 catcgttgcc agtattacta tgatcatttt cgtctgtact tttatattgt tcataagatt
    10801 ccccttttct gtttgtccgt ttttgcagat ctcctacctt aattttatgt ttatcttatc
    10861 acattttaca attctcttca actttgtgtg gtctttttgt gtattattgc caacttttat
    10921 tcataatcag ggaaatttca ccatgaatat ctgagatatt tccggacaac aaaaaacctc
    10981 agtagatttc tctactgagg ttcctattcg caggtcaacc cctgcttcgc agggctacaa
    11041 ggattcgaac cttgaaatga cggagtcaga gtccgttgcc ttaccgtttg gcgatagccc
    11101 tatgtcgttt acttgatgaa gtatacacta tctgttcctt ttttgcaata cctaatttaa
    11161 aaaaaattta aaaaaatata tgaaattgga acaatttccg taaaatcatc gcataaacac
    11221 cgtgatatag gggtaatcct tcctctctgc aggcttaaaa tcctccatca accagttcca
    11281 gataaagctg ttctctcctt cctgggcaat atctccataa cagctttcaa ataccaccac
    11341 attcggatat ctgtccggat tgatcttcca gtagtccaac agacgctcat catagatcat
    11401 agagcagatc gtatcggggg atgcctgctt atgttctccg agcatacaat agaactggga
    11461 cggtcccaca taaagcacgg tactgccgtc cggcacggcc tccggccata cagccagatt
    11521 attgttgtaa cggtatgcag tcatataatc cgtgaggatt ccggcgcgta atccctctct
    11581 gttgatgcca cgcacatttt gaatcacata ggcatgatat tcctgggatc ccagaatcag
    11641 ccaggtatag gaaaatgcat tcccaagcac aaggatcagg cacaggatct gaattgcctt
    11701 ctttcctgcg gtgttttcct tctgccagta gtctcccaga cataccagtc cggccactgc
    11761 tcccataatg agataggtga ccagcagcat cggtccccag ttagacaaca gaagtaccga
    11821 aaaataattg acaaatgcca gcaggatcag ttcatagaac agcctttccg ttttgtctct
    11881 gcgcagatag atataacatc ccgtcaggga tactgccatc agcaaaagct gcgggtaaat
    11941 tgcattaaag gaactgaaca gccagaacca gatctggatc agaaatacaa ttacgaaaaa
    12001 cagcatgccc ggaagctgca acagtccggg aagttttttc ttccatacct tctctataaa
    12061 ccatcggatt cccagagaca ccagcagggc acacagaagg atcatggcca tctctcccag
    12121 gcttgacccc catcccaaca atttttctcc cacagtagtc tgatgggacc cctctcccag
    12181 gatctcgcct gccatctcca acattttctg cggtgtcata taactcagaa gatatgtaaa
    12241 cattactgcc gcacttgcca cacagggcag tacataagca cagagttctt tccatttttt
    12301 ctctgatctg cgcaccagca gaaataccag gcagcacaaa aataccagta ccatacttgg
    12361 ataagccagc acatcacagg tcatgaatat acctgccagc accagccacc gcagctctcc
    12421 tgctgtctgt ctgccctcgg agtccttcgc tccgaagtat ctcagaaggc acagcaccat
    12481 aagtgcaaag aaccagttat gaagattgga atactccgga ataaaaatgg acttgggtgt
    12541 tgtcacatag tagagaagcc ccatcagtgc tgccacattc tcatccatct gcggcactgt
    12601 cctatggatc gtcttataca ggaagacact gacacctgcc tgaatcggga aaaatacgag
    12661 ccgtaaaaat agattcagat aattcactcc acctgtcagc ttcacaaaca gcctgataaa
    12721 aaccgccgta aaaatggcag aagtctggtg tggttcccac atttcaagca gcagccggtc
    12781 tcccatggca agacgataac ccgccatgat gccatagcct tcgtccgtat ctgcaccaat
    12841 aaataaattt tttgccgtat atgcaatgat tgcaagaata cagataattt tccaaaattt
    12901 cttatttttc agtatatttt tcatcatgca gttatcataa cacaggattt tccccatagc
    12961 aatgacaaga aacatacata actgcaaaac gaggctcacc atcatatggc aagccccgtt
    13021 tttattcaac acaatgagag aaacgtatta tggtatttta caatgctgtt tatacttcaa
    13081 agatcagatc accataggta ggtaccggcc ataccttctt gtcaaccatc atctccagtt
    13141 catctacggg agttctcagg tcatccattg cggtatgtac cacatcttta tagaagaaag
    13201 caagcttctt gggatcttcg atgcctgctg cctcagccag cttatcctcc agagcagtct
    13261 ctgcagcctg tgcctcagcc agcttctcgg aaatgctctt cagtacagtg gtctgtacgg
    13321 ttgcatccgc acctgcactc ttaacagcca gtacagtatc cgccagagtc ctggtatact
    13381 ccataactgc gggaatgtac tgcttcttcg ccatatctac catggtcaga gcttcgatgt
    13441 tgatggtctt ggagtaggtc tcatacagaa cctcctcgcg ggattccagc tcagccttgg
    13501 tgaagatacc gaacttctcg aacagtttga tagacttctc ggtggtcagg gtctcggaag
    13561 cctctaccat ggacttgatg ttgggaagtc ctcttctggc tgcctcttct acccactcct
    13621 cagaatagcc gttgccgttg aagatgattc tctgatgctc ggtcaggtac ttcttgatca
    13681 gatcgtgtac ggcaatatcg aaatcgtctg ctttctccag aatgtctgct gcctcgcaga
    13741 aagcttctgc aacgatagcg ttcagagtgg tgttggggct ggcgatggag tctgcactac
    13801 ctaccatacg gaactcgaac ttgttgccgg tgaaagcaaa cggtgaagtt ctgttacggt
    13861 cggtggcatc cttatccaga tcaggcagtg tggatacacc ggttaaaagc ttaccgccct
    13921 ggatggaatg agtagcatca ccggtctcca ccagctgctt taccacatcc tccagctgct
    13981 cgcccaggaa tacggaaatg atggcagggg gtgcctcgtt agctcccagt ctgtgatcgt
    14041 tgccaacatc ggatgcactc tgacgaagca gatctgcatg ggtatctacg gctttcagga
    14101 tacatgccag tactaacagg aactggatgt tctcgttagg agtatcgccg ggatccaaca
    14161 ggttgacacc gttatcggtt cccagggacc agttgttatg cttaccggaa ccgttcactc
    14221 cggcgaaagg cttctcatgc agcaggcagg tcataccgtg acgtcctgct actttcttca
    14281 tggtctccat aaccagctgg ttatgatcta ccgcaatgtt ggcggtctca taaataggag
    14341 ccagctcgtg ctgtgcggga gccacttcgt tatgctgtgt cttggcggtt acgcccagct
    14401 tccacagttc aatgttcaga tccttcatga aggaaccgat tctctcgcgg atagtaccga
    14461 agtaatgatc ctccagctcc tgtcccttgg gtgcgggtgc accgaacagg gtacgtcctg
    14521 cgaagatcag gtcttcacgc tttaagtatt tgtctctgtc taccaggaaa tattcctgct
    14581 ccggtcccac gctagctacg accttggtag cttcggtatt gccaaacagt cttacgatac
    14641 gcagtgcctg tgtgctgact gcttccatgg aacgaagaag cggtgtcttc ttatccagtg
    14701 cctcaccctt gtaagaacag aatgcagtgg gaatgcagag gatcacgccg gttgcatcct
    14761 ctttcaggaa tgcgggagaa gtgatatccc atgcagtata gcctcttgcc tcgaaggtag
    14821 ctcgtaagcc tccggagggg aaggaagatg catcgggctc gcccttgatc agctccttac
    14881 cggagaactc catgatcatc ttgccgtact catcgggatg ggttacgaat gcatcgtgct
    14941 tctctgcggt gataccggtc agaggctgga accagtgggt ataatgggtg gcaccgttct
    15001 cgatggccca atccttcatt gcctttgcta caacatcagc ggtggccaga gacagctctc
    15061 cgccgtgatc aatgatcttc ttcacttctt tgtatacgct cttgggtaat ctttccctca
    15121 tcttacccag agtgaatacc ttactgccaa agatttcttc cacgtttaat acttcactca
    15181 tcttcgtttc ctccttattc ttgctatttt ccatgcttcc tgcgacttac ttgcacagtt
    15241 gctattcaga acttacatgc gcaaaatcgc atcttcgatg ctttcctttt gcttatgtaa
    15301 gttacctcgc cgaataacta tcgttacatc ttcttgcgca tgcaagcatt cgccgaagat
    15361 gtacgatagt tacggctctg aatagcaaca aaaaaaggcg ctacactttc tagtgtaaac
    15421 gccttcgttt cgcattgaaa aatattaact tgtgaggatc gctcctctat ggctcattac
    15481 tatacaacag attttttttg aatgcaatac cttttttgaa aaaattgtaa ctattcagaa
    15541 ctccattcgc aaaatcgcct ctacgaggct ttacttttgc atccgaagcg atctctgaag
    15601 tgctctatac ctcaaacaac agatccgcat aggtcgggaa cggccagtac ttcttgtcta
    15661 ccagcttttc cagttcatcc gcaggttttc tcagtgcgtt cattgccacc atgacttcat
    15721 catgataaca gaatgcctgc tccttgcctc tctccatggc ggaagccttt ttctccacct
    15781 cgagaagtgc ctcggatgcc ttttccatct ctgtcagctt ctgtgtgatc tgctgcaaca
    15841 tacgggtagg cacgtaggtg tctccgccag cttccgtaat agcaataaca gcatctgcca
    15901 gcgacttgct gtatttcatt actgcaggaa tgatctgctt gtttgccata tccaccatag
    15961 tcagtgcctc gatattgatg gtctttgcat aggtctcgta cagtacctcc gcgcgggaac
    16021 gcagctctgc ctcggtgaaa ataccgaact tttcaaacag atggatagcc ttgggagtag
    16081 tcagagtatc tactgctgct accatggaag gaatattggg cagtcctctt ctggctgctt
    16141 cctcttccca ttccctggca taaccgttgc cattgaaaat aatacgctgg tgctccgtca
    16201 tatattcctt gatcagatca tggactgcca tatcgaaatc ttccgcacct tccagacggt
    16261 ctgcagcttc gcagaacgcc tctgccacaa tggcattcag ggtggtgttg gggcttccga
    16321 tggaatcctc gcttcccacc atacggaatt cgaatttatt tcctgtaaag gcaaacggtg
    16381 aagtacgatt tctgtcggta gcatccttta caaaatccgg caatgtggat acaccggtgc
    16441 gaagtacacc gccttctttc agattgtctg ctttaccggt ctccaccagc tgtgccacca
    16501 catcctccag ctgttcgccc aggaagatcg aaataatggc agggggtgcc tcgtaaccgc
    16561 ccagtctgtg atcgttacct acattggacg cactctgacg cagcagatcc gcatgggtat
    16621 ctacagcttt catgatacat gccagtacca gcaggaactg aatattttca ttgggggtct
    16681 ggccgggctc cagaaggttt acaccattgt ccgtagtgat ggaccagttg ttatgcttac
    16741 cggaaccgtt tactccggca aaaggcttct catgcagcat gcagcgcaga ccgtgcttgc
    16801 ctgccacacg cttcagagtc tccattacca gctggttgtg atccactgcc acattggcag
    16861 actcgtagat gggcgccact tcatgctgtg cgggagccgc ctcattatgc tgggtcttgg
    16921 atgttacacc cagcttccac agctcgatat tgacatcatg catgaaacca ccgatacgct
    16981 cacggataac accgaaatag tggtcatcca tctcctgtcc cttgggggcg ggagcaccga
    17041 acagggttcg tccggcgaaa ataatatctt cacgctgcaa agccttggca gaatctacca
    17101 ggaaatattc ctgctccgcg ccaacggaag gcagtacttt ggtagcttct gtattgccga
    17161 acaggcggac gatacggatc gcctgcttgc tcagagcctc catggaacga agtaacggtg
    17221 tcttcttatc cagtgcttcg cctttataag aacagaatgc tgtagggata caaagggtcg
    17281 gaccggtagc ggtctctttg ataaatgcgg gggaagtgat gtcccatgta gtgtaacctc
    17341 ttgcctcaaa ggtagcacgt aagcctccgg aagggaaaga agatgcatcg ggctctccct
    17401 tgatcagttc tttgccggaa aattccatga tcatccggcc atcctcatca ggatgtgtca
    17461 caaaagcatc atgtttctct gcggtaatgc cggtcaacgg ctggaaccag tgggtataat
    17521 gagtggcacc gttctcgatg gcccaatcct tcatggcact tgccactaca tccgcagttg
    17581 ccagggaaag ttctcctccc tgatccatga tcttcttcac ttctttgtaa gtactctttg
    17641 ggagcctctc tttcatcttg cccagggtaa acaccttgct ggcaaaaagc tcctctacat
    17701 tcaattcttc gcgcatgtcc ctacttccct tcggtataca ataaaataat atcgaataca
    17761 gtgtacaaca tgtgcgttat aatggtcaat tattattttc gaggttttag tgataagttt
    17821 ttccaaaaat actctatact cagaacactt cccagcagga ttccgggcat ctgattgtac
    17881 atctgtcttc cgctggcttc ccagaacatt aaaaaaacaa cgataccgat aaatcccaac
    17941 tgacagatca tcagcattac aggtggttcc tgatctctga taatattgcg aaggctgagg
    18001 atcattccga tcagtaacag cacatacatg gacaccagat agcagaaaca atactgacag
    18061 gtgcgccagt agtattttcc ccagggcgaa aacatttccc agagcagggt tccatcgctg
    18121 tactccacgt tcaaaaatgc actggccccc agatttccgt tggcaaaatt atagcttgcc
    18181 tttttgcgca aatgtacggg attccagaat tccgatctgt tttcacgaat atattccata
    18241 ctgtattgct gtttttcctg tctggtggaa aattcataca tatgatccac aaactctgtg
    18301 ttgttggtcc aggaaccgtc ttccttcatt cccagtgcca cccagctgat cacaggattg
    18361 gctgtttcag ctgcttcctt tgtgatctcg taggaattgg cccagagctg cagagccact
    18421 ccaagaatca cgacaaaacc tccaaacagc aatgtcactc gacggtccac cgacactctc
    18481 tgccacaaga ggatcacagt gcatgcgatc agcggtatga tcactgtgat cttccatgtg
    18541 cctgccagta ctgccatacc ggccgcaagc agcgcccaca ggatctgccg gcgcttcccc
    18601 ctggatgctg ccgccaattt caggaaagcc agtcccagga ttcccatacc gaagctcatg
    18661 gaatccgtat aaaacacggc agtcatctcc cacatcggaa gcagaaatac aaaggcaagc
    18721 aggatcggaa atctccagca agtatcccca ttccgttccg caagataacc acaggcacat
    18781 gccaccagca tcaccagaat tacattccgc agcaaaacaa aataaaacgg ggacacatcc
    18841 atcagcaatg ccatccgaaa cagaacggaa agcaaaagca tcggcttcag attattcgaa
    18901 taggtcaaaa aataattggt gtcttccagt tctctcccgt tcgccagatt cagagcatcc
    18961 cgatagacat aggtgtaatc cagcagagta ttctcattcc cctgccggct caatgaaacc
    19021 acataaagca gcaccccaaa caataccagc gctacggcat acagcgaatt tctccggcct
    19081 gtcaccgtat atattttatt ccatgccgca ccggccgcca gcagcaacac tgtagcagcc
    19141 gccaccccca gagccatctg ccatgggatc agatgattcc ccggatacct gagtactgtc
    19201 cacaacagca acagcataaa agcgatgaga atactatagg cacctatgga cactccttta
    19261 tgaattctgc cttccctttt cataaggcgt tacctccggt cactctttct caatcagcat
    19321 ttttctgacg cagcaaatca aattcctgcg gcggcatgga taactttatc cacaacacct
    19381 gcttgggatg gggtgcactg tccacacttt ctccctcctc ggtgtacata gcttccaccg
    19441 tgaccggtac attggtgccg tcaggcttca tgatttctat ggaatcgccc acgcagaatt
    19501 tgttccgctg ctcgatccgg cacagagaat cctttactgc ctccacaatt cccagataaa
    19561 tatattcgtt cacgtaagtg ttattatcat agatctgcgt attttcatcc ggcttgccga
    19621 aatagaaccc cgtcgtaaac tgacgatagg tgcacttgga gatctccgcc ttgtaccagt
    19681 ccatattttt ctcgtacagc tccggatcgg caaaataatc atcaatggcc ttccggtagg
    19741 ttctggctac cgttgccaca tacaatgcgg tcttcatacg cccctcgatc ttgagactgt
    19801 cgattcctgc ctccaccagc tccggaatat gttcgatcat gcacagatcc ttggagttga
    19861 aaatatatgt cccccgctca ttctcgtaga ccggcagata ttctccggga cgggtctcct
    19921 ccaccaccgc atatttccag cggcagggat gtgtgcaggc acctctgttg gcgtctcttc
    19981 ctgtaaagaa attgctcagc aggcatctgc cggaataaga gatacacatt gccccgtgga
    20041 caaaactctc aatctccatt tccgcaggaa tcttctcccg gattcccttg atctctacca
    20101 gtgacagttc tctggcagac accactcttt tggctcccag cttccaccag aacagatatg
    20161 tctcataatt ggtattgttg gcctgcgtgg aaatatgcac ctccacctgg ggccagactt
    20221 ctttggcgat catgaacatt cccggatcgg aaatgatcag cgcatccggc tgctccggct
    20281 tcatatcccg cagctcggca aaatattttc tggctccctc gagatcataa ttgtgcgcca
    20341 gaatattggc cgtcacataa accttcactc catgtgcatg ggcgaatgca actccttccg
    20401 ccatttcttc actgctgaaa ttttttgcct tggcccgcag tccaaaagct tctccgccga
    20461 tataaacagc atccgcgcca aaaataacag ccgtttttaa tacttccagg ctgctggccg
    20521 ggattaaaag ttccggtttt ttatatgtca ttttatttct cctctttttc tgcaacagat
    20581 tctctttcct gataagcaca ccatgtcaga tttagcggaa gcattatcat caccggcagc
    20641 atatagcgca gttctccgtt gaccggggaa agaatgcata ccgcaaacac cagcagtgtg
    20701 ggtactatca gcaaaatccg ctttcccttt ttcgatccca gcagatacac cacatatccc
    20761 agcagcaggt atccgtgaaa tcccgcatta tacaacaggg agatcagcgg gaattgtgtt
    20821 acaaaatgta tcatgccgat cagaaattct ctcatgcctt tcagctgtgg aggaaatcct
    20881 atttccatat agaactcttc catcgaaagc tgctctctgc caaccgtagt ctctatgatc
    20941 ggcgttccca gtctgtcctt ccggtccgta taaaaatatc cgtaagtctg gttaaaaaat
    21001 gcctggaaat aagtatccgg atgcttgcag aactgtgcaa accagacttt aaaataattc
    21061 ttcagttgtt ccttcgtggg atgggatatc atctggtctt tggccgcatc ggatatctcc
    21121 ggtgaataat gtgccgttcc catctgatca tagtcatcaa acaattccga cagaatcgcc
    21181 cgttcttcct ccgtgacctc tgctccgtga tccctctggt atcttgccgt ctgttgaata
    21241 ggggccgata gtccttccgc cagtctcccc tcttcaatat catgtgccgg catatatatc
    21301 tgctctatat agaacaggat cataaaaccg gttactgctg taaacaagta taatttccac
    21361 tgctttttat gatagaaaaa cagacagatc agcgcaccca ccaaaaggta tctgccttct
    21421 tttctgcaga ggatcatacc gtaggaagaa tagaggaata cgatctgttt ccaccatttt
    21481 ttcttttccg ttccatctac caggatatcc gcaaatgaca ctccaaaata caacaggctg
    21541 atatagtact gtgcatcctt caccatagta aatccccaca taggaaatag cggaaacaca
    21601 gtaagaaaca gaagtccgaa ccaacggatc cagaccggag ctttcatttt tttcataacg
    21661 cagactagat atgagattcc aactgcctgc aatagggact gtatcaaagt ataggtaaaa
    21721 attccaagat tgtcggatcc cagtacgact cttccgaaag ccagacatct tcccatgatt
    21781 gccgtggata ccaccgggtg tttattgctg ttttcaataa ttcccatgta cctccacacc
    21841 tgttcaaatg catccgcctg aatcggtccc gggaaaaaac ttatcatata gggcagcgca
    21901 cacagcattg ctatggcaaa agtactccag aagggatggg cttcgaaaat aaaattctct
    21961 attttcccct ttatttctct cctgcgcact ccttcccaat gttccgacac ataggctccc
    22021 aacgcaatga gactctcata caggttgtaa taaccggcca tcaccaccag cgccaacagg
    22081 aactgagcaa agtttccaaa cacatagttc cagctgccct gctcatgata gctcgtcccc
    22141 attaccgtag taaccgcaaa aaaggcgcac aaaacatgca gccatctgtt ttttctcaga
    22201 gcttcatcct tcgtgatcca gcatactgct cccacaccca tggtcagaaa aaccatttcc
    22261 acatgcagac cgtccaatcc gtcactcaat gttgtcagac agcccggtgt caacgcaaat
    22321 atcatgaata agatccggaa aaaccatcca acgtttttcc atttactgtt ttccttaagc
    22381 aactgtttca tctgctgtac ccttcttatt tctccaatat ttgaacgctt tattcatagt
    22441 ttccaaaaga atgagataaa tcaaagcatt tcccagccag taaccaccta ttatgatcac
    22501 tttatccgga tgtatcttat aataatatct ggttgtagcc tgtgcatctt ccgcagcaaa
    22561 caacccgccg ttcctttcaa gacgtatatt ctccggggcc acaccgtttc catgttccgc
    22621 atacataaac cggatattgc attctgcggc ttccggtatt gtaagcgtcc aatgatatgt
    22681 ctgttttccg gaaagtgaca cccccagggg aagtgcgtag aaatgatccg cactcatact
    22741 tccgagagga aaatcctgtg acatcagctc tgtattcttt tcatcatata gtacaaaatg
    22801 caatacctga tctgtcaccg cttcctgctc aaatcccagc gcaaattcta tctgatccaa
    22861 tctggaatac agcggcttaa aatactgtgt gatctccgtc cgttccgtga tgggctcact
    22921 atacacactg tattcactgt aagagctgct catatagtta ttatacacca ggtatcccgg
    22981 ccagatggct aattccagtg caatgagcag caccgtcagc agaccttttc ctatgacctc
    23041 tgccttgttg attttctttt tcttttgacc cgacatcata atatataagg atacacgaga
    23101 tgaagaattt atgcaaacaa aactggtatc agatcacatt actttttctg ttttgtgtca
    23161 cagtattttt aagattctat aaactgaccg ttttaccgga tgttttacat atagacgaag
    23221 ccgctcttgg atataactcc tggtgtctgg ccaattacgg taccgatcgt tatctcaatg
    23281 tgctgccttt ctatccgcag aatttttacg gtggtcaaag ccctctctat acttaccttg
    23341 tcgtattgct gatcaagttt ttcgggaagg ggaatctgtc cgtttttctg gtaagattcc
    23401 tgctgccttt gccagtattc ttctgtggat caccggcacc aggagcatgt cccttatctt
    23461 tgataataaa aaaatcaccc tcgctgctgc tctttttctg tgtttctgcc cgtattttgt
    23521 gatgtccgga cgttatgccc tggactgcaa tctgatgttg tgttgcattt cctgctctgt
    23581 tcttgtactg cttttgtatc tgaaaacagg aaaactgcat actttacttc tgtccggagt
    23641 ctgcttcggt atcagcttgt actcttatgc attgagttat ctgatcatac ctatttttct
    23701 ggttctgatc tctctttaca tgctttacca taaaaaaatc acgttttcca gaacactgtt
    23761 gtgggcggtc agtgtctgca ttacggcatt gcccgtgatc ctttttgcct acacacttct
    23821 cttccatgca gagcctattc attttctcgg ctttacaatt tctcctattt ccggcaaccg
    23881 catggatgaa atttcttttt cctcattggg tgcaaatctg tggaacaacc ttaagatcac
    23941 tctgacatac agtatttatc ctatggattc cgtggataaa ttctatacct tatacccggt
    24001 ttccatccct tttatcctgt tgggaatgtt atgttccgtt tatgacttcg gaaattcctt
    24061 ccggacacgc tgttttcatt ccggcacagt atatctgttc tatttcatca gctgctcctt
    24121 tgtggttgct ctgacaccta cagatcatct atatcgtgcc aacagcatct atatctgtta
    24181 tctgttatct gttctttttt ctccgtggta tccgggcatg ttgcgatttt tttaccgttt
    24241 accggaaagc atttctgagc attctggctt atggctatgt gctgtggatt gcttcgttta
    24301 tgcgttacta ttacactata tattccgtgc tggatctgca tacctatgca aattccttct
    24361 attttgccga tatttccgac attgtcacct atattgacga aaatctcggg gataaggaaa
    24421 tctatgcgga ctgcgttgat gtagaagagg ttttgtattt ttattatcct atttctcctt
    24481 atgagcgcgt tcccctgtcc gcccgggaag aagacgatga aattcccaag cattatatac
    24541 tgaatgcttc cactccgctc tctccttccg cagcctacat tgtccgcaaa gaaaatcagg
    24601 aatttatttc ttatctgaac gattccggac tttcctgcca tacggtagaa ttttcaccgt
    24661 attacttatt ttattttgat tgattgcgag gtatccacaa atgaaggatc tgaaatctct
    24721 ggtgaaaaaa tactggtatt ccgcagccct cctgctgttg tttctcattg cggtcttcct
    24781 gcgcgtttat cgtctggaaa gcctgcctga cgttcttcat atcgatgaag cagggctggg
    24841 ttataatgcc tggtgtctgg ctcattacgg aaccgaccgg tatctcaatg tacgcccttt
    24901 ttaccctcag aatttttatg gtggtcagag tcctctttat acctacttac tggctctgct
    24961 gatccggaca gtcggacagg gcaacctgtc tcttacgctt ctcaaaattc ccgccgtcct
    25021 tgcaagcctg ttgctgtttt ttgtcgggac caaaagtatc cgtctggtgt tcgatgatca
    25081 aaaatggagt attgctgctg ctttcctgtt ggcagtctgc ccctattata tcacgtccgc
    25141 ccgttttgcc ctggactgta atcttatgtt gtgctgctca gctgtagcat tgctgtttct
    25201 catccgtttc acacagacca agacccttcg gaaccttata ttatccggag tcttttttgg
    25261 tatcacgatg tattcgtatg cactgagtta cttcctgatc cctatttttc tgatctgcat
    25321 ttccctgtat ctgctataca cgaaagaaat atccttccgc cgggttctgc tctgggctgc
    25381 atgcgtatgt attacagctc tgcccgtaat attatttgta tgctccctgt tattccactg
    25441 ggaaaccatt cattttctgg ggcttaccat atctcccatc gcctccgagc gcatgtccga
    25501 tgtgggagtc acttctttct gggaaaatat aaaagatatc ataaagatta ccttgacatg
    25561 tggttcctac cgtttagatg ccgtacctaa attttacacg ctttatccct tatccattcc
    25621 ttttattgtt ctgggattta tcggtgcagt ttacagtttt cttgtctctc tgtacaagcg
    25681 gacttttcag gccgacagta tctatctgct gttctttctc agcggtctca tcaccattgg
    25741 tctgtccgga tccggctatg tctaccgggc taacagtttc tttatctgtt atctcttctt
    25801 cctgataaaa ggacttttcc tttgctgccg tttcctatcc tcttatcaca caggctttat
    25861 gctccttttg tccgccggtt atctcttatg gtgcatctct ttctgcagct tttattttca
    25921 ggtctatacc atgaccgacg tctgcccgaa ttatctgtat tccatctcct tccgggagtg
    25981 tgtggatgat gtgaatctca acctggatta tgcggatctc tatgtggatc tggtcaacat
    26041 gaaggagttt ttcttctttt attatcccgc atctccttat gatactcagg atgaagaaac
    26101 ctttaccgac ggtatacaca cttatcattt tctcaccgac tattccacga agatcagtcc
    26161 ttcttccgtc tatgtggtat acaaaaagaa caatgaattc ctgcagaatc tgtcagagtc
    26221 cggactggct taccagacct gggaataccc gtattattat gttatttatt ttgagtaacc
    26281 gtgctttcct ctttcttcgt actcagtgtc actccatcac ctaccggcag gatcaccgtc
    26341 tccagtgccg gatgatgttt caattcatac agatattccc gcatccttgc atgaatggtc
    26401 cggttccttc tggttacggc aaaacgggat tctatgatat caccgtcctg taatacgttg
    26461 tcggagatca acagtccgcc ggggggcaac aggcgtaaga tatccggcag gaaatgaatg
    26521 tactgtccct tcgccgcatc cataaagatc acatcatagg tgccttccag ttcccgcagg
    26581 atctccgtag catcaccctc cagcagagtg atccggtctt ccctgcctgc cttacggaag
    26641 ttctcttttg caataggaat tcttttctca tatttttcga tcgtcgtgat atgacaatcc
    26701 tccggtgcat attcactcat cagtaacgca gaaaagccga tagcagtccc gacttccagg
    26761 atgctcttcg gctttgtaaa tttcagcaaa aatttcagca gactttgcat ggatctgcgt
    26821 atgatgggaa cctgtgtctc cagagcgaat ttttcaatct cattcagcca cgccggattc
    26881 ccgctgtcca gggaatcgat aaacgcggac attctctcgt ctatgatcat actgttcctc
    26941 atttcacact ttactgtgca actgcagtgg tatcctgtgt ctcaccggta atcgcactgc
    27001 cgttgtctat agtctccact gtctcttccg ttgcgctttc cgtctgtccg gccatgactt
    27061 ccatcatctc ttctgcggtc atggctgtac tcaattcgta ggttccgggc ttccagtcct
    27121 ttttatactc ggatagcaga tactgtaatg cgaacagtct gctgtcacgt gtcaggccct
    27181 tttcctgcat aattgtgcca atctccgttg cggacatgtc cgaggtcagg gttactgtga
    27241 tcttacgtcc ctctccggca gagaccgccg gttctgtgaa aatacgataa ccatagtcgt
    27301 agcatgtaga cgctccggtg tacaccagat ataccacgat cactgcaaat gccactttta
    27361 agatagcgcc caatacggca cctgccaaat ttgctgcctt catgtgttct gtcctttctt
    27421 atccaagtaa tccgcatttc gatccataca ttcgcaaaat cgtctcttcg agactttcct
    27481 tttgctcact caaaatgcat ctgatcggta acgtaaagtc cgatctcctg catcatacgg
    27541 caaaatgcca gctccgccgc cagaaaatcc gacaccagtg gattctcccg gaaagcttcg
    27601 ttttctctgg taaactgatc cagcttatca aagttggtgt ccttgctggt ctgcaattca
    27661 aaatttctgg tccggaattc atcgatctgt ctcttaagtt ccgattgttc cttcacgcgg
    27721 gccagctgct tctgatattc cagataagcg tcggtattac gaatcgcctg taccatctgc
    27781 tctacagcct gatttacatt ttcgtccgat gtaatcttca tctgcttacg acctttccag
    27841 caatcgctgc tattacactt ccataatgat cggaaggatc atgggacgtc tcttggtctt
    27901 cttccataca taatcgctga ggacatcctt ggtggtggtc ttcagtttac cccagtccgt
    27961 aattccctta tccagacatg cctgcagcgc atcatccacg gtgcgtctgg cttcctcgat
    28021 aagttcatcg gattccttca catatacaaa acctctggat acaatgtcgg gacctgctac
    28081 cagttctcca ctggccttgt ccagactcat gaccacgatc ataataccat cttctgccag
    28141 atgctgtcta tctctcaata ctacatttcc gacatcaccg acacccagac cgtcgaccag
    28201 aatagctcct acgggtactc tgccgttgac cttggcctgt tcctcgctga cttccaatac
    28261 atctccggag gacaggatga agatattgtc cttactgatg cccagactct ctgcaatctt
    28321 ggcctgtgcc ttcagatgct tgtattcacc atgcacgggt actgcgtact tcggatggat
    28381 cagggtatag atcagcttga tctcttcctg gcaggcatgt ccggatacat gagcatcctg
    28441 gaaaataaca tctgcgcctt tgcggagcag ctcgttgatg atcttggtga cggacttttc
    28501 attgcccggg atggggttgg aggagaagat tacggtatct cccgcaccga tcgtgatctt
    28561 acggtgcata ttgttggcca tacgggacag ggctgccata ctttcgccct ggcttccggt
    28621 ggtgataatc acctgctgct catcgggata attcttcatc tgatccagat ccaccagtgt
    28681 attctcggga atattgatgc actccagctt gatggcggta tccaggatat ttaccatact
    28741 gcggccttcc acagccactt tacggccaaa tttgtatgcc gtattgatga tctgctgtac
    28801 acgatccacg ttggatgcga aagttgccac gaaaattctg gtgttcttat gttcttcaaa
    28861 taaagtatca aaggtcttac ccactgtttt ctcagaaggg gtaaagccgg gacgctctgc
    28921 attggtggag tcgcacatca gtgccagtac accctttttg ccgatttctg caaaacgctg
    28981 taaatcaatg gcatctccat ataccggtgt gtagtctacc ttaaagtctc cggtgtgcac
    29041 cacaataccg gcaggggaat aaatggccag tgctgcggca tctacgatac tgtgattcgt
    29101 cttgatgaat tccacacgga aatcgcccag attaatggac tgaccgaact tcactacttt
    29161 acgcttggtc ttcttcatga gattgtgctc ggtcagcttg ttctcgataa tacccatggt
    29221 caggcgggta gcgtagatgg gaacattaat atcccgcagc acataaggca gtgctccgat
    29281 atgatcctca tgtccgtggg tgatgacaaa accctttacc ttgtcgatat tgtccttcag
    29341 ataagtcaca tccgggatga ccagatcgat tcccagcata tcatctgcag ggaaagacag
    29401 accacagtcc accacaataa tactgtcctc atactggaag gcagtaatgt tcataccgat
    29461 ctgctccagg cctcccaaag gaatgatctt cagacctgaa acgttactat tctctttttt
    29521 ctttttcaac gaaattacct ccaaattcta tacgtctata catcttgctt cgtgtatgta
    29581 ttccggcgtt agaatgagat agtatgctct gcaaacaaag atctgtgatt tcacgcctgc
    29641 tccttccggc tctcctctca aagactcccg cggagatcag acatcccaga tcttacgatt
    29701 ccatcaaaat aacggcatct ctcatgccct tcgtgtattc cctgtcactt ttggcacaaa
    29761 accattatat gctgaaaccg gcaaagcctc caatatccct ctctgccaat catacaaatc
    29821 atatataaat ataagaccgt ccttctcgca ggaccgcctg tatttatcat atatcaaggt
    29881 cataagcctg aaaacacaac gtgcttgcac gggagtgatt ttatggctta atgacctgcc
    29941 ccaatatgag cataaaagcg gagcgaatgc ttcgcaatat ggtgatattg gaacggattg
    30001 tgtgagtgca tggcacggaa caatccatag gatatgatct aaattaaatc aacgtcctct
    30061 aacatatccg caaatactcc ggataccgct tccagttctt catcatcgga gactacannn
    30121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    30181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncat atccgcaaat actccggata
    30241 ccgcttccag ttcttcatca tcggagacta catcataaat gctctcttct tcaccatcct
    30301 gagacatatc cttcaggatc agggcatctc catcgccctc ttccacatcg gttaccagga
    30361 tgtaattgtg tccaccgatc ctggtctgtt ccagtacata aaattctaca ggttcttcgc
    30421 cttcggggcg aaatgtgatc ttttccaatt tactcattct gtttcctttt tcttctcatt
    30481 gtttctgcga tccagatagc cctgcaggat cagagtagct gcgatcatat ccacgtaatc
    30541 cttacggttc tcacggcgga ttcccgcttc catcatggct ttgtccgctg ccacagtcgt
    30601 cagtctctca tcccagaaat ataccgggag accggtcctg cgctccagct tgtccttgaa
    30661 ctctttggtc agttccacgc ggacaccttc ggtatcgttc atgttcttgg gaagtcccag
    30721 tacgatctct tccacttcat tctccacgat cagttcttcg atccgggcca gcgtctgtcg
    30781 taatttattt tcttctttgc ggcgaatgat ctccacaccc tgcgctgtca acagcagact
    30841 gtcactgatc gccacgccca cggtacgtga gccgaaatcc aaccccataa tacgcatgtg
    30901 ggtcctcctg ataactgtaa ttatttccag tgttttgtcc tgatatactc cgtgagcagt
    30961 tcttccacca gttcgtcacg ctctaccttc atgatgaggc tgcgggcatt tttgtgactg
    31021 gtaatataag tagggtcccc gctcatgatg taacctacga tctggttcac cgggttgtat
    31081 cccttttccg tcagtgcctc atatacggta gaaagcacca gcttcacacc ggtctcaggt
    31141 tctgtctcta ccttgaagta ttgtgtattt cctaagtcct gcatacgatc ctctttttct
    31201 gccatagggc aaatctttcc gttatgcctt atattaccgc atttactttg aaaattcaag
    31261 gggtgatagt aataggattt catccgtcaa aaacccctgt accctgccgc atatcacggt
    31321 atttgcaaca ttttttttat tttccttcac agagacagcc agtttgacat aatctcttgt
    31381 atgtcccagc aggtattccg ttccgccgat ctccttggtc tcctccagaa gcacctccgc
    31441 ctcccgtccc acatagtggg tccggaactg cttcgactgt tccttctcca tagccagaag
    31501 ttccgcactt ctggtggtct tcacgggatc cggcacctga tgagacatca cggccgctct
    31561 ggtgcctgcc cgtttggaat acttgaaaat atgcatctcg aagaattgaa tccggtctaa
    31621 aaattccttt gtctgcgcaa actcttcctc agtctccccc gggaaaccca caatgacatc
    31681 tgtggtgatg gcgggatggt caaatacctt acgaagcagc gcaacactct ccgcatattc
    31741 ttcggaagta tatttacggt tcatacggcg tagcgtggca tcacagccgc tctgcaagga
    31801 cagatggaaa tggggacaca gcttcggcaa tgccgccatt ctggctgccg tctcctccgt
    31861 aataatacgg ggttccaggg aaccgatacg gatccgctca atcccttcaa tagtatggat
    31921 ccgttccagc agatcgatca gcttactgct gccggaataa tctcttcttt cctcgtcttc
    31981 ttttaataca gaaacgcttt ctccccgctt ccaggcctcc tcgtcgaaat caatgccata
    32041 agaactgata tggatacctg tcaatacgat ctcccggtat ccgttctgtg ccatgccggt
    32101 gatctcccgg atgatatctt ccgccctgcg gctgcggact ctgcctctgg cataggggat
    32161 aacgcagtaa gagcagaact gattgcatcc gtcctgaatt ttaatatagg ctcgggtgtg
    32221 ctccgccgtc tgtttcagag tcatttcttc gtattctgca gtatggttaa tgtctataac
    32281 agtagtgtca tgcagagtct tgtccacacc atgtccggca ttttctgcat tctctgccac
    32341 cgcatcttcc tgttccagat cctgcaaata ttcctccaga atggatgcca ggttcttctt
    32401 gcggttgttg ccgatcgcca gatcgatgca gggatcctgc tcaacatctt cccttccggt
    32461 ctgcacgtag catcccacag ccaccaccag tgccgccgga tttttctgct tcgcacgatg
    32521 aagcatctgt ctgcttttcc ggtctgcaat attggtaact gtgcaggtat ttacaatata
    32581 aatatctgcg gtttcatcaa atggtacaat agtataacct ttttcttgta acatttgttg
    32641 cattacttct atttcatagg aattaacctt acatccgagg ttgtgtaatg ctacactttt
    32701 cataggattc ctcacttttt ttgtattgtt ttcgtcttga cttttgagcg ttcccccatt
    32761 aatataaagt cagaaggtat gacacacatt aaggaggtag tcaacatgaa gacagttcag
    32821 atttctttaa attctattga taaggtaaaa tctttcgtaa acgatattac taagttcgat
    32881 tatgatttcg atcttgtatc cggcagatac gtaattgatg caaagtccat catgggtatc
    32941 ttcagcctgg atctttccaa gcctatcgac ctgaacatcc atgcagagga tggcgctgat
    33001 gaggttctgg aagtattaaa gccttatctg gtataagtta ccgattcgga attccgaata
    33061 catagtaaaa tagtaaaaca gagaacacct tgagagacgc aatcctcttg aggtgttttt
    33121 tatatgccat tccaaaaaag taactattca gtacctctgt ttccccggtt ctgaatagtt
    33181 actctgcatt cgcacttatg cttccgcaat gatcagttct ttggtctcca gtgcggtctt
    33241 caccagttca tcgatcttct catccagatt gtgttcatgt ctgcggatca ccttcacgtt
    33301 gggtttcgtc acaggatgct ttgccacgaa aatggtacag cagtcttcat aaggaagaat
    33361 ggaagtctca taggttccga tcttctcgga tacttccacg atctcctgct tgtcaaaacc
    33421 gatcagcgga cgatagaccg gcagctcgca gacttcattg gtggcgatca gggaattcat
    33481 ggtctgagat gccacctggc caatgctctc accggtgatc aggcccaggc actcagtctc
    33541 tttcgcgatc tgttccgcaa tccgcatcat ataacggcgc atgataatgg tcagttcctc
    33601 atgaggacat ttctcataaa tatataactg aatatcggta aaattgatca catgcagata
    33661 gatcggtccg gtgtaacggg caacgatctt cgccagatcc actactttct gcttcgcacg
    33721 ctcactggtg taaggcggtg catggaaata taccgcatcg atcttgacgc ctctcttggc
    33781 gatcatataa cctgccacgg gagaatcaat accaccggag agaagcagca ttcccttgcc
    33841 accggtacct acgggcattc ctccgggacc gggcaggatc acggagtaga tatagatttc
    33901 ttcacggatc tcaatgttca gcataatatc gggatgatgt acatccacct tcatattggg
    33961 ataagccttc aggatagctt ctcccatatc catgttgatg gtcatggaat ccttggggta
    34021 atttttccgg gcacgtctcg ctgccacctt gaacgtctga ttgcaatcgg gatatacttt
    34081 tgcaatgtag tcaatcacgg tctcacacag cttctcaaag ccctcatcct gtacgtgcac
    34141 taccgggcag attccggaga ttccaaagac tctttgtaag tgtgccacaa cttcatcgta
    34201 gtcaaattct gactctgcat ctacgaagat acggccctgt gtcttgtgca ccaggaattc
    34261 tccctcacac tttttcagtg catacttaat ctggcgtacc agagcatctt cgaacaggta
    34321 acggttcttt cccttaatgc cgatctcggc atattttatc aaaaatgatg taaacacgtt
    34381 cgtttccttt ctgagtgttt ctcaaatcgc ttatctgcga ctgtatctgc gtaacatagg
    34441 tactatattg tacaatgcct ggagacatac gtcaatctct tcttccgtgg taaatacgga
    34501 aaaactgaaa cgtatggtgg aatccagcag ggatttatcg atgccgatgg ctttcagtgt
    34561 agcggaaggc tgcggcttgt gtgcagagca ggcgcttcct gcagaaacat agattccctt
    34621 gtcctccagt gcatgcagga gcacttcact tctgacgccg gcaaaagata cgctgacaat
    34681 gtgtgccgcc gttccctcac cgtacggtgc atccggcaga agtccgttga ctgtcacctg
    34741 atccagttct cttacgccct cgataaatcg tctcttgcag gcgcagagtc tggcatgatc
    34801 ttcatcaaaa tgactataga gcatttccgc tgcttttgca agtcccgcaa taccgggcac
    34861 attctcggta ccggagcgga ggttctgctg ctgtccgcca ccgaatacga tgggctggat
    34921 cttcacccgt tcccctacat acagcagtcc gacgcccttc ggtccgtgga tcttgtgtcc
    34981 actgacggac agcagatcaa tgtgcatctt cttgggatag atccggaatt ttccgaatcc
    35041 ctgtaccgca tccacatgga acagcgtatc cgggttcatc tgtttgatca gagctccggc
    35101 ttcctcaatg ggctgcaggg ctccgatctc gttattcgtg tgcatgatag ataccagaat
    35161 ggtctccggt gtcatggctc tgcgtaagtc ttccagccgg atacgtccgc agggatccac
    35221 cggcagataa gtgacacgat acccctgggt ctccagataa cgcatggtct gcaggatagc
    35281 gggatgttcg atctgcgtcg tgatcagatg attgcctctg cggcagttcg ccagggcgca
    35341 gccgatcagt gccatattat cggattccgt tcccccggaa gtaaataaaa gctctctctc
    35401 ctccaccttc agaatccttg ccagagtctc cttggcataa cggaggtatt tctctgcctc
    35461 cactcccttc atatgcaggc tggagggatt accgtaatct tcacacatga tcttcgtcat
    35521 caactctgcc acttccggga atgtccttgt ggtagcggaa ttgtccatat agacttccat
    35581 tctcttcttc tccttgctat ctatctcttc agtaactatt cagcgcccta ttcgccaaat
    35641 cgactcttcc gggctttcct tttgctctga ataattacta tattcttctt atgattaaaa
    35701 tatgccgcag gcaggcgatc cgtgccgtcc gctgtctaat tttccaactg atacatcagc
    35761 catgcgatag tcgtaaatcc ggccgtctcc gtccgcagga tccgttttcc caaagtgatc
    35821 ggttgaattc ccgcctccgt ggcaagtctt atctcctccg gatcaaatcc gccctccggg
    35881 ccgatgaaaa tagcgatctg ctgtccgggt cttgcgcttt cgatgagctg tttcgtctgt
    35941 ggcattcctt ccgccagctc gtaaggtacc agtttcagat ccatttcaga agccgttttc
    36001 accgcctgca cataactcat aaccggtgcc accgtgggga tcacgccgcg cttactctgc
    36061 ttggctgctg cctccgcaat tccctgccag cgtttgattt ttgcatccgc ttttttctca
    36121 tccagcttga ccacgcagcg cttcatggac accgggatca cctgaaaggc tcccagttcc
    36181 acggccttct ggatgatgag ctccatcttg tccgccttgg gcagtccctg gaacaggtag
    36241 atcttcgccg gaagctctac tccgtcttcc ttgataaaac gaagctccag acggacacag
    36301 tcctcttcca gtgcaaggat cccacagcga tattccttcc cgtcgattcc attgctgaca
    36361 gccacttctt cgccggggcg catccgcagt acatttttga tatgattcac atcggttccc
    36421 cggatgataa cgctcttcgc tgccacgtcg atctgttccg gttctacaaa aaactgatac
    36481 atagaaactc ctattttaag cacttaattc tttcttgcgg taacgctgac ccattctccc
    36541 tggtaggtca cttccagtac ttccaatcca gcggccttca cagcctccac cacggtctgc
    36601 tccttgtcat caatgatgcc agaggtgatg tagataccgc ccttcttcag ctgatgtacg
    36661 atgacaggag tcaacggtac cagtacatct gccagaatat tggccaccac gatatcataa
    36721 cactcatagc ctaccttgtc ctgcacttct ttgtctgtaa tgatattgcc gatcatcatg
    36781 tcaaactgtg ccggatcgat tccattcact tcgcagttct gtgccacagc ttccacagca
    36841 cagatatcca gatcggttcc cacggcatgt tttgccccga acatgaggga taaaattgcc
    36901 agaataccgc tgccggtgcc gacatccaaa agctctgtct caggagtgat gaacttacgc
    36961 agctgacgga tacacagctg tgtggtctca tgcataccgg tgccaaatgc cgtaccggga
    37021 tcgatatgaa gcaccatctt gtcagtatct tccggtttga tattttccca ggaagggatc
    37081 accaggatat catcgatata gaactggtgg aaatactgtt tccagttgtt gatccagtca
    37141 atgtcctccg tctcatctac cacaacagaa gcctcgccga tatccataaa ctggcgtaag
    37201 tcttccagtt cccgctttac atccgcaagt acagcttcgg tcgtggtctt ctccccggcc
    37261 acttccagac tgccgtcctc tttttcctct acaaaaaaac ttaaataggc aatgccgtca
    37321 tcctccggtc cgtccggcag gatatctaca aacatctgct ccttctccat ggcggtcagg
    37381 ggcaccttgt cttcgatctg tgccccttcc agtccgagat cgtacagggt gctgataata
    37441 atatcttccg cttccgttac cgtctttatt ctgaatttaa cccacttcat tgttcttttt
    37501 ttcctttctt ttccattgtt ccaacacagc atatatcatt gcagccacga ttgcaataaa
    37561 actgtcgatt tccaatacgc tgaccgcatc cagcttctct ctccaggtgt accggttcaa
    37621 tgcctgtccg tccacagttg cttcatgtac caccagttgc tgattttcca gtacatgcgc
    37681 cttttccgta gtagtatata ctacctgcgg cagattctcg gaaatacttt gattcacgat
    37741 ccggtattca tacagggcac cttttttcaa tcgggtctca atgaccgccg ggaaaaaagt
    37801 caccggttcc atggcatcat aaggaatcac accttcatat atcttctgat cctctttcca
    37861 tatctccaga cccagaagtc cgttccgttc ttcctcctga ttatagttca ccgcgatttc
    37921 cagtgttttc agaaaatctg tctccgcaac aaaatactgc gaagcatatt ctcccacctg
    37981 caggggctct gtatatccat aatcctcact taaggaactg tcctttttat cttcacggat
    38041 tcctccatac ggccatacaa acaacatgga aagtatcgct gccagcagga ctcctctttc
    38101 cacccattta atcatttccc gtttcctccg ttatgttcac ctttaaaata tcaaaatccg
    38161 tattcccatc catatactga aatccataat gtcccatatt ttcataggtt acggtatatt
    38221 tatccgattc ctccagcaga tgtccgaaac actggaaata ccagactctt ttccccacca
    38281 gttcttccag atccggcacc tcttccatgg tataaaagat ctggtccggc atatagcatc
    38341 cgaatagctg tccgaagcgg gaatccgtat cgtagacgac cacatctccc ggctccatat
    38401 tctccgctat aaatgccttc gtctcttcca gatatgagat tgtaccatat tcttcatgat
    38461 aagagattcc gtaggccgac actcccatcc acacagtgaa ggacagacag gcgatccatt
    38521 cctgtaaatt gatcctggat aaaatgatag ccattcccag ccagaacaat cccatgctat
    38581 gcataacatg gcggtaggta aaaaagtggg acgcgtgtaa tgccagcagc gccgccatca
    38641 aatagcttac tgggaaaata caggtacaca gaaccgcaaa aacaccgttt cttctcccca
    38701 gccgcttcca aaagaggatc gcgcagacca gtgtcagtac aaaaagaagt tctcccattt
    38761 tggtcgcata cttcactccg gttccgaaaa tgaaatcaaa aaattcgtaa ataccgaatt
    38821 tggaccggtt cgtcgggttt ccatcagaaa ccagcagatg aaagaaatcg gattttatca
    38881 gccatgccac aaaaagcaat acaacggaac caccacagac aaacggcttc cagatctgtt
    38941 ttatgtcctg ctgccaaatc accgccacca gatacaggta gataaacagg taactgaacg
    39001 cggtaaagta atgactgaaa ataccgcaga aggtcgtcag aaacaatatc gtccacaggg
    39061 ccttactgtt cagccgctcc cgcaggaaat aagcggtcag tccgcagacg gtaactccaa
    39121 acaccgtcca ggaatacatt ctgttgttca cactgatctt catcattgca ggtgtcaggc
    39181 ttaccatgat catataaagg gctgctgtct tcgctcccca ctgcctgcgg atcacaacag
    39241 gccccagcag catggacaat accgttccca gcaatgcaaa cattttgtag gcaataatgc
    39301 cgtctcctac cagaaacgac cacagccgca atcccatata ccataccggc acatccgtat
    39361 catgggccgc cagtaccacg cgaatgattc ccatcatcgt attgtcatgg gcggtgcgat
    39421 aactgtatgc ttcatcatag tcgatcccct cactgagaca cagggagaac aataccgccg
    39481 tgcacagaat gaggatcagc cataagccaa tggttgtaat tttatgtttc ttttccgaag
    39541 tcattttttt catcttcatc agatcattaa ccctgtcttc tctttactct tctctatcat
    39601 atgattcagc ttgcggaacg gcggctccag tgccagtccc agcagcaatg ctacgatcag
    39661 gaacagtgtc atttttccta cattgatcca ataatccacg ccgtacattc ctgcaataca
    39721 ttctttaaac gctttcatgc caaactggaa cggcaggaaa ggatagatcc actggaatac
    39781 ctgcggaaga acctcgatag gaaacgttcc gccgcttccc gccacctgga tgaccatgac
    39841 tacaatagcc agtgcctcac ccacgtttcc aaaagcgaaa gtcacagaat acataaagca
    39901 gctgaatgcc gcactggcca cggctgccgc cagccagtat aaaaacctgt gctgacactg
    39961 tatctcaata taataaaggt tcccaagaac aataagcagt gtctgtgcct gtccgatcat
    40021 gaaaaacagc agatatctgc caaaatatct ctcatgcgtc ttataatgat cccccggttc
    40081 aatgggtttc acacccacgt gaataatagc caccaggaac agcgctccca cccagattgc
    40141 caggatcgta tagaacggcg atgccgcaga gccgtaatga tccaccggat aaacaggtac
    40201 cgtctccagt ctgaccggag aagatacgaa gtctgccacc gccgtcggat cggcagccat
    40261 agcgcttaaa atatcctcat aggtctccgc accggtgatc cgttttatat tatctgctat
    40321 tttgctgacc ttgtccagca gttcctgtgc gatcaccagg gaatcctgca ggctgacatt
    40381 accttcctgc aaagtctgct gatactcttt cagaatatcg gaaatttcat ccatattcac
    40441 atccgtaccg ttcatcatcg ctgcggcatt caggaaagcc tgctgcatgg atttgccggt
    40501 agcccggaaa gaaggtctga cattgttatt gtaatcactc atcagattat tcagctgttt
    40561 gttgcagatc tggatctgtt cccggatctg tgcccgcaga gtattgatat catgatccac
    40621 atctcccttg ctgtcttgca gctgtttcag atccgcttcg atcgttttca gagactggtt
    40681 gatctcctga atctgagcat cctgtccggt gatcaggctt cccagcacat ccatattggc
    40741 ctgcatgccc tgtaccagtc cgatggccgt gcccaatccg gtgtccgtgg tgcttcccac
    40801 attgttgctg cccatctgct ccagcaatcc atcagaaaag accgacagat actgcaacgc
    40861 ttccgtgacc atatccatac tgctgctcac catatctccc ataacgtcaa ccgcatcgac
    40921 gctggaattg atcatggtct gcataccgat catggtattt cgtccattct ccagcatatt
    40981 atccatatcc ggaagcatat tctggctggt ctccatgatc atctccgcgg aggtcataat
    41041 actctggaag gactgtaaca taaccacata gtcctgcaac tgtgcctgca tggtatccag
    41101 agattcttcc acactggtct gcccgccggc attcaccacc gaaagggcat ttcccgcttt
    41161 catcaccgct tctgccacag tactgataaa ggtggcattt acctgttcct gcacagcatt
    41221 tttcgctttg cccgtgattt tgggggcaat accattcttt ttctcatttt catagtagta
    41281 aatggtggga tgatccactt caccgtcgat aaatcccagc atttcctgag taaaagtctc
    41341 cggaataatc aatgccgcat agtaatcact gttcttcacg ccttccagtg cttcctccgt
    41401 ggtctccggg aacacccagc cgatagtggt attggctccc agagcctcca gcacactgtc
    41461 tcccacgttc agagacagcc ccatgatctc cgccccggca tcatcggaag ccacggctac
    41521 tttcagctga ctggtagctg cgggctcgta aggatcccag ttggagaaaa tgttgaacca
    41581 ggcatacagt gccgggagga tgctgagtcc catgataata acgaccgcca ccacattatt
    41641 gctgatccgg cggatgtcgg tggtaaatat tttccagata ttatacatac tattcaccat
    41701 cctcccagtc ttcggtctcg tcttcgtttt cctcttcggt ttcatcttcc ggatcctctt
    41761 cggtcccggt ctcggccggg gaattcaggc cttcaccaat cagtttccgg gtctcccgga
    41821 tgatctgcgc cgcccggtca aagtctgtcg gagtcaccgc tttgagatcg ccttcttctc
    41881 ctttgagctc catgatcttc tcctgcagat tataatccat atattccaca acgatcagat
    41941 agattgacag ggcaaacagc gacacgatcc acaggatcag gaagatgacc ttgttgctgc
    42001 cagtcaggaa caacagtgcc agaaaaaaca aggggacgat ccagatacat ttcagaccga
    42061 tctttattct ttttctattc ttctcatgaa gggcctgctc gtaatccaga accttctgaa
    42121 aaatttcttc gtaatttctc tccatgattt tacctttctg cctgttcgga acttcattca
    42181 caaacccact cctgcggagc tttcgttttc atggttctga acaggtgtca cattaactca
    42241 gtgtcttcca tacgctcttc cacaaaatgg ttcagtttta cgaacgggat ccggatcacc
    42301 aggccgatca acagtccctc gatcataaac aggctcaatt ttgccagtgc ttccatatag
    42361 gcagtaccgt acatacccgc cagtgcctca cgcatggccc ggatggcata ggggaaggga
    42421 aagaaaatat aaatcttctg gtaggtgtcc ggtagcagtt cgatcgggaa ggtaccgctg
    42481 gaaccagcga tctgcaatac cataacaata acgcagacgg ccttgcccac atctccgaat
    42541 gccagtgtca gagaatagat caacagggaa aatgccgtag ccgtaaagct tgccgccagg
    42601 aaaaacagcc ccggttccac aatgtttact tttaacagcc acagatctcc ggccacaatg
    42661 atggctgcct gaatctgtgc caataagaag aacagcaaat atctgccgaa atacaattga
    42721 tagctctgta cattttcaag ccctttcgga gaggctttta ctttcaccag tgccaccagg
    42781 attgtagaac ctacccagat cgccagtgtg gaatagaacg gtgtcatggc agaaccatac
    42841 gtagccaccg gatacatggc tatggtctcc atggttacgg gagatgcaaa atatgccccc
    42901 agactatccg gatctcctcc aaggaaacgc accagaatat ccatcatttc ctcttcggat
    42961 gcaccgtcca gcttctctgt cagatctgta agtttggcgc tgatattatc taccacatct
    43021 ttgatctgtc ccagactctc actggttccg gacacagtgt caccgacacc ttccatgaca
    43081 actccgacat ttttcaccgt attgctcagg gtatttacca gattggtcac ctgattcaga
    43141 gaatctgaca tatttgtcag aaccttctgc atctggggca ccagattatt cttatagatt
    43201 ccctgcatat tacttacaga agtctcgcag ctgtcaatgg cgctccttaa attattcatg
    43261 gcattgttga cattgacctt tgcattctcc gcagccttgt cgtccgtgga ttccagaacg
    43321 ctgcccacta cggtattcat cagatccagt tccttctgca tggctttcaa tgcttccata
    43381 gccgcatcag ttgccgctgc gttgatattg ctgccggtgc cgccgttcgc agcattaccg
    43441 tcagagacgc tttccagctt ttgttggatc agtgccgcca gcagtgtatt tacttgtccg
    43501 ttcagcgtat tggtatctct tgccacccgg ttcagatctc tcgtcatctg ggcggtatcc
    43561 tccgccagtt tgctttcatc cagagccttt ttgatacttt ccagagagtt attgatattg
    43621 ctcatggaag tatcgatgtt gttgctgaaa ttgcccaggg tgtctctggt agaagacaga
    43681 tcctgactgc tctggttcag actggctgtg gtgctgtcca gtttcttctg catcgcggga
    43741 atctcatagt tgacatgggt gatggcagac tccagccgcg cattgcctgc ctgaaaggag
    43801 tcgatcatgg cactgtattc cttcagattg tcattcagtt cctccagttt ggaacagaat
    43861 tcctctacat aactgtcccc ctgtacctga tccgacaccg tattgccctc ttcaaatacc
    43921 gtggtagccg ccaccttgat aaaggtctca ttgatggtat tctgcagagt tcctgccacc
    43981 gtatccgtga ttttggtggc aatggcattg ctcttctgat tctgataata gatcagcgcc
    44041 ggattctcaa aatcatccga aaatacattg tacatactat aagtaaattt atcggtaatg
    44101 acgacggctg cataatattt tccggcttcc acgccctctt ttgcttcttc ctcggtatcc
    44161 agaaagatcc attttacact ggtgttggaa tgcaggcttt ccagtacgga ttctcccata
    44221 tttaccaccg atccgtcttt cagcgtatag tcctgatcca gggatactac cgccacagga
    44281 attcctccgg tattggcata gggatcccag ttggcaaata tattaaacca cgcatacagc
    44341 gacggtatca cgcacaaccc gataataata accagcgcaa aaggctgctt caccagtttt
    44401 ttcagatctc cggtaaaaat cttccaaata tttttcatat agcttctagc aactcctcat
    44461 ccgtatccat ttgattggtt ttggtctttt ctccccgggc cgccgcttca cacaatccca
    44521 gtatcaggaa gaaaagcggc gcattcagca cctgctgaaa gctgatcagg gaatgtatcc
    44581 cgtaggtaag cagtaacaat actgcgagaa aattcttcct gtatgtctgc agcgccgtga
    44641 taaaaacagc tgcataggca gccacaccca gcagtcccat attgaccagt gtggtgagcc
    44701 actcattatg tgcattggta aaaacagaac cttcaaaata tcctttatca aaaagcaccg
    44761 tgcctcccgg caacagttct cccagatacg atgcaaaaca atccgggcct gctcccaaca
    44821 gcttctgatg cagatttcct ttccggaaac ccatccatgc catctgccac aatcttcccc
    44881 ggcgattgcc ccattccgca aaatcgatta ccgacaaccg atgcagatac catgccagca
    44941 ctaccgctat tcctgccatt gaacaacaca aaactcaatc ttccaatata ttttt
//
LOCUS       DAWDHD010000004        43802 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_77_length_43802_cov_12.698859, whole genome shotgun sequence.
ACCESSION   DAWDHD010000004 DAWDHD010000000
VERSION     DAWDHD010000004.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 43802)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 43802)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..43802
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77_length_43802_cov_12.698859"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT        10025 a        11733 c         9469 g        12575 t
ORIGIN      
        1 tctgattgaa agcagcatct ctggcaatct ctgccgcgat aaccatctgc ccacatcttg
       61 ctgccactgc agtaataaat cgaaggaact taagcgaatc tatggcatcc gataattctc
      121 ttacatcccg ctcaatataa gtagacagat aactgctgta aaaaagctga ctgttactgt
      181 taataccgct tacgatcgca ggcatggagc cttgataaat ccgttcaaag atctctcttg
      241 tatcagcttg ctctctctct ttctgcctct cgtttaaatc ctccagatct accgtaaacg
      301 gtttcatcgt tcctccggat atctctgcct gtgacaggga tgtaagtgaa agtactgcca
      361 ctcttcctgc cagtgactcc tgtactccct gcatcaattt gaacacctgt gatcccgtaa
      421 gccaaaatgc tcccggctca tggtttttat ctacatttat cttgatataa gtaaacagtt
      481 ccggtgcata ttgaacctca tcaatcaata caggtggttt atgcagttgc agaaacagtt
      541 ccggatcggt ctttgcaata tttctttcgt ttaaatcatc cagtgttaca tatccccgct
      601 ccgttccctc catcagtttt tggagcattg tcgtctttcc tacctgtctc ggtcccgtta
      661 ccaggagtac cggatattcc ctggttacct gtcttactac attctccaga tttcttttga
      721 tataactcat aaaagtccct ctttcggcta atctatactc tgattatatt ttagccgaaa
      781 ccacattttt attctttcac attgattccg tcataaaaaa gggaagccga tatctctgtc
      841 agctcccctt catctgttgt atttactttt tattgtcgta catctgcggt gctacgtatc
      901 ctgctccgct catgctcttg taatagtcat aagcactttt ggcgtttttc cgtctctgtc
      961 caatttcggc acgttccctc gcaaaataac tctcgatgag ttttttgttc cgagcctcct
     1021 gagcctgtac agtcacactg agttctgtca ccctgctgac cttcttctga agttcccgga
     1081 tctgtgctgc gtagcgttct ctgttgcctt ccaactccct tgccagcttc tcatacatga
     1141 tttcaaagcc ttcatccaga tgaaccacct catcaataag ccgctgtttt tcctccacag
     1201 catcgtcaaa caggctcata tccacctctt ccgcagaaaa gatctcttcc tgacgtttgt
     1261 tatattcccg gattatctgt aatacttcca gcttcttgtc caggctctct gacaaaatgg
     1321 taagctgatt ctccatcagt catttccttt ctttgctccc gccttcatga cttctttcca
     1381 gttgtcacgg atagcgtgca ggtgggtatt gatctcttcg aggatttcct tatctttttt
     1441 gacattggct tctaccagac gtctctcgag ataagtgtag atgttgtcaa agtccttgga
     1501 gacttcgtat ttcatgtcca gcgtggaatg cagatatcca atgatattct gcaccttgcg
     1561 gatattttca tgtgccttgg gtacatcgga tttctccaca gccatctctg caatattgca
     1621 gaacttgata gctccgtcat agagcatcaa tgtcagttct gccggagaag cagtcagaac
     1681 cttgctgtta ttatactgtg cataagcacc gggtaatgcc atatcatgat cctcctaaca
     1741 agctggtcac cgcgctggaa ttgctctgca gctttgccag tgccgtctcc attttggaga
     1801 acttggaata ccacttgtct tcataatcgt tcagcttatc ttccaagtct ttgatcttgc
     1861 ttgtataatc atcataatcc gatttcatct ttttgtcatc ataaaaatta ccgtaggtac
     1921 gataaccttc tacagacttg gagagatcac tcattttagt atataaattc ttgaacattc
     1981 cagagaaaaa gcttacgacc gtatcgggat cactgctgat catgctctta agcttgtctg
     2041 cattaccgga ggtgctggag tcatctgcat ctccatcaat gtggtaggca ttcttctcat
     2101 tctccgctgc ggtaaagtag ctcaaagtct caataccgaa atcgctgaga tacatgttct
     2161 tgccgtttac ttccacactg cctgccatga tttcttttaa ggcagagctt acggtagaca
     2221 gattgctgtc tctgcggagc agggcatcct taatcttggt ctcatacttc tctacttcgg
     2281 tatcggacat ggcatccttc tcatcgtcgg tcagaggctc atatcctctt gcggaatccg
     2341 cattataaag cttgtccatc tcgttgatca gggtattata ttccttcagg aagctcttga
     2401 tcatatcgta gataccatct acgtcatcct gtgtggtcag ggtgatctct tcatctgctc
     2461 cggtctcttt cagtgctgta atggtcagac cattgatgga aaatacattg gagttgctgg
     2521 tatactctgt gttgttcagt gtgatcacag aatcctgccc tttgatatag ttggcatctg
     2581 taatacccag ccccttcagc aggtcatcgg caccggttcc ggttgccgta atagagaaat
     2641 taccggcatc gccggagtcc tttgcactga tatagaatct ctgctgggtg gtatcgaagt
     2701 ttgcattcag tccggcttcc ttcaatttgg acagtacatc agagatcgtg gtatctttgg
     2761 taatattcag ttctacgctt gtatcaccgc tctggatttt aagcgttgta tcgtctccgg
     2821 tggtaatgcc ggccacggtg ctgccatcac tgcccttgat ccccagatca gacaattttg
     2881 tcaatgcatt cagcttggta tccgcagtga cattatcctt caaggtcagc ttgccaccgg
     2941 tcatataggc ggtcttggcc agctgtgata ccttgaggtt ctgtacaccg gtcattgcgc
     3001 catcaccggt gatgacactg actgcattgc tgtcggatac tttggtcact ctcttgctat
     3061 atgcggattc aaaacgcata ttattaacaa acttggactg cagattttta atcttggtat
     3121 tcagctcttt ccatgcggtc tgcttccatt cctgtttggt ctgtgccttt ttcgcatcgt
     3181 ccaccttggt ctgcttcgca gacaccagct gctggataat gctctctgtg tccataccgg
     3241 agatcagtcc ggataatctc attgttgatg atgccatatt ggtttcctcc tatctcttct
     3301 catccaccag gatgcctgcc atctcccata ctttggcgat catgtccaaa gtcttctcag
     3361 gtggaagctc tttgatgact ttcttggtat ccttgtctat gattttgatc gtgatgcggt
     3421 ttgtcgcctc atgaataccg aaaacagctt ctgaatggga catcatgttc ttattcagct
     3481 tctctactgc tttcttgatc tgctcgttgg tcacctgctg ttgctgttcg ctattagcat
     3541 cggcagaagt attattctga tccttgctct gcgcattttc aacaactgcg gtggtctggt
     3601 caagtttatc tgctgctttt acattggcag gattgtattc tgagggattc tcctctgtag
     3661 ttacttttgt cgcagtgttc ctggcagcgg ctgtattctg tgcctgcact gtcatgacac
     3721 tggaaatcgg ttctatggtc ataatggtca cctccgtgtg ataaacaggc gccttccgtg
     3781 ggcgcacgac cttgcttctc taaggactgt atcggtaaat tctcccgaaa aattaataga
     3841 agatgcaaaa atttgcacct cctatgccta atttcctatt tttctatttt ctaattccaa
     3901 tacaggttca tattctctgt aacgctttct tctgacagtt gaaatcgagt ctttactttc
     3961 tcagccgtag tattataatc cgcccccaat tcaatgcatg ttatgatcaa tgccttaata
     4021 ccactctgcc gttcctcctg tcttccttct tgtctccctt ctcgctttcc catccttaca
     4081 tattgctcaa ataattcaca catagtgatc tcatcctcct ctctgatatt catttccttt
     4141 aaaatagcag attctgttgt ggaatcctct tcaccggata atacattgat cattctgatc
     4201 aatgccgctt tgtgtaccac cttctggtcg gaacgaagat tattgccctc tgccagataa
     4261 tccagcacaa ttctcatatc gctatgaaat ctctcccgca cctcttgggg caggtaacgc
     4321 atttcccaga tatgtaccct aatatcatcc acatattttc gaagttcttc cggcactttt
     4381 ccacaacata attcatggat tcccggttgc ttctcccagt gctcccttcc ccagtaaagt
     4441 atcatttcta taatgggata catgtccatc actttgcctt cgtactgctc ccgatatcct
     4501 ccgccggtat aacccgcttt acgcaacacc attttgacat ccggtgccga ctgattggcg
     4561 atcatatatt gtgccctgat ccgttcccct gccatctcgt atttcaccac atcctgtaac
     4621 tggtttcgca gcctgttttc tccgctcgta taaatacttt ccgtcggtgc cggcaacaga
     4681 ttttctttgg tcacaacaag ctttccatta tgcagcaacg cattaataat gtctgcagca
     4741 acatctggaa atatctcgaa attctttgta acaaggtcgt ttttctctct gttttccctc
     4801 attttttctc ccttaagcac actaaactta cctaagcagg aaaaactgaa accgttccat
     4861 gcactatttc cctttccgcc tgcttatgtt ttgtcatttt gtttgaattt ttggcgaaaa
     4921 gcccgaaata cacacaaaag atattgtcag aaattgtcat aaagaattct ttagatgttg
     4981 taactatagc ataaccctat cgtcttttca agttcttttt tccgaaggtg tatcaatgcg
     5041 tatatttaca gttttaggaa aatgtgccct tatttttcaa caatggataa aagctccgga
     5101 tttatgcatc cggagcctct ttcgtttcct atttttattt atttttaccc caacaggttc
     5161 ttcagtgccg ccatgccttc ggcatccaca gcggccttgt tggcctcctg gatctggagg
     5221 tagacttcct tgcggtagat gggcacttcc ttgggtgcgg tgatgccgat cttgacctga
     5281 tcacctttga cctccagaac ggtgatctcg atatcgttgt tgatgacgat tgcttcgttc
     5341 ttcttacggg acaatgccag catcttactc acccgccttt ccggatttca ggatgtcgta
     5401 taccggatat tttaccgggt atttatctcc ctcaaggatg atctggcagg ccttgcgttc
     5461 ttccacgttg atcacaaaag gtccctgcag attcaccgtc atcttcgtca ggtcggcagg
     5521 caccgtcaca gtgaccatga ccagaatatt ctccggggta agattgccca caccggtaag
     5581 cagctcgtca tccacctccg gatcatattc cggatggact accagcggat ccatgaccgg
     5641 catggcgaat ccgggttctt ccagagactg taaatagcgg attcctacgt tgctgccctt
     5701 ctcctcatcg tggagcatgg taaaatgctt cagttccgga aatccgataa tacctttgtc
     5761 aaaagtgatg atcttgtcat ccgcaatctc aacttctcca aaaaattttg tattgatctt
     5821 cataaaatac ctacgccttt ccgtaacctg ttgttctaga tgaaattcaa cagtgtggtc
     5881 tgcattactt taccggtagc catcagtgcc gcttcatagg tcatttccgc actcttcaga
     5941 tcaatggcgg tctcggtaac atcgatatcc tcgttttcac tctttaaggt ctcaaaggtg
     6001 gtcttctgac tcttcatgcg gttatcgatc agttccagtt tgctgcttct ggtaccgcag
     6061 ttggtaacgc acaggttggc atcatccaga tatccctgga acgtagtaat gccgctttcg
     6121 aacatcttct ggcatttgtc ctttgccagg gtcagtgctt tttctactgc ttccatctgc
     6181 ttcgtcaggg tctttgcatc cgtaccggta gcagtctcct gcagtccctc gatcttggtc
     6241 ttcacattct ccagatccac cacatcctgc atggcctgta ccagatcgtc caccgtacgt
     6301 ccgataccgt gctggaagca ctcatctgca gtggaattca cctgaatggt ctggttgaag
     6361 cctacatcat attcgatgac ctgttttgcc gcatcaccct tgaggtattc ctgattatat
     6421 ttcgttactt tctgatccgc atccgtactg gtacatgcaa aataatgctc aggtctcaga
     6481 tcacctttga cccaatgatc cttctggtag gtcacacgga tctcgccttc gttcttgcca
     6541 gaagtggacg gatcgtcctt gcaggacatc agcttttcat aacggttctt accaagcagg
     6601 acctcaccgg tatccggtac atagatcagt gcatcatcat cctttgctgc catagaataa
     6661 ggatccgcgg tagaggagac cgtctggaac atcttggaat cccatgtcgt catatccttt
     6721 ttataggcac cgtttgcatc tttctcccag aaagaaatcg aaggttttgc atccacatct
     6781 ctgcagtcat cataagccag ccggatacga tacacatcta tggagctgac tgaattttcc
     6841 gtaatatcct tgtagtcctg attctgatca ttatagttat tcgccgtaat atccagcagt
     6901 tttccggtat tcacctttgt cagctgtgtg atggagctgt tgtccagctg ctcggtgatc
     6961 tcatatttcg tattgtattc tttatcaaaa ctcagagaag tatcggtacg atatccggta
     7021 aacacataac ggcccgcata gtctgcatcg ccggtgctgt acacttcatc tcccagggct
     7081 ttcagctgtt ccagaatgat ctggcggtct gcggatttca gatctccgtt agatgccttg
     7141 gtgcactgtt tgatcatatt cgtgatgatc tctgtcaggt tcttcagtgc atcctcggtt
     7201 acgtccagcc agctctccgc atccggaatg ttcttgctgt aatactgcgt cacctcggtc
     7261 acattgctgc gtaaacgcag ggcacggatg gataccacag gatcatcgga aggacggctg
     7321 accttcttct gggtagacat ctgggtgctc agcttatcct gatatacttt gttcgtattg
     7381 atattggaca gattattacg ctgcatgatc ttgttcgtaa ttctcatttt ctccgtaact
     7441 attcaggatt ctaaaaatca gaataactgg tacgttatgc ttgcatataa gtaacagttt
     7501 tctgattcga atggtgactc gaatagagtc accacacacg tgagcaaaat tagctgcgta
     7561 gcagcgagaa agcacggata cgtgcgtttt tgcgaacata tgtgggaatc cgaatagctg
     7621 cctcctttct agtttagact ccggtctcca gaatcagtct gtcataaatc tccgtcagtg
     7681 tctggatcat cttggatgcc agattatatc cattctggta ttttaccaga ttcacagctt
     7741 cttcatcctc atccacaccg gagatggaga ttctctggtt gtcgatggta ttggaaatgt
     7801 ctttaaagct tgcatagaaa gtattggctc tggaagcatt caatgccaca tccgataaga
     7861 tacactgtaa aaattcggaa gcattacatc cgcggaagct catcttgctc ttatccgtag
     7921 ccagattctt cagatcgttc aacagatcat tctgttccac gccgtcagat gcattttttc
     7981 ggtttgccat caggctggga tcctgctcca tggcatccag aatatcgaaa ttctccgctg
     8041 tcagacgata gtagctgtca tcgttgactt ttaccgtaac tttactgttt ttcttctcct
     8101 gcttgtcata tctcttggct gcatcatcca acaggaactg ctcactgctg gttgccttat
     8161 tgccggtaaa catcttcaca ccgggatctc cgcttccgct gtagccggaa gtcagaatat
     8221 cgttgaattt ctgggaaaat gtacgaatcc actcattcat ctgattcata taataaggaa
     8281 ttccctgata ggacaggtcg gtaccgatct ctgccttttt ccctacacgg tcattggtaa
     8341 ttccccgggg attcttctca ctgtccgata aagtaaatgt ataggagtaa gtggcgttgc
     8401 cgttcgcatc atattcgcag gtatattccc aggaatcata gtagaattcc tgattgccca
     8461 gatccaggat gcctccctga tccgacagat tacatttatt cagatcctgc agataagcct
     8521 tggttacttt tacggttacg gtatcatggg tcttgccgtc tgccgtggtc gtcgtgccgg
     8581 tggcggtcac ctgtccggtg aagttctcgc cgttgttacc gtcacgcatc tggatcagtc
     8641 ctgccagatc accgcccatg gatgcgttgt acagattaaa cttctgcccg tccgcccagt
     8701 agacttcata gagaccgtcg atatcggtct ggtttacttt ctcataggag gttcttgcca
     8761 cacattcgag tccgttgtaa tcactgccgt ctaccagcat ctgtccgcct gcgatcttga
     8821 ccatatatct gttggctccg gtctcacggt tctcgttgtt ggcatcgatg atcggagtct
     8881 ccttgacttc cacatccacg atcttggaca attcatcgat cagcagggtt ctgcggtcac
     8941 gcagctcatt ggcctttgtt ccggccagtt caatggtgtt gatctgttta ttcagggagg
     9001 cgatctcacc ggcaagggaa ttgatctgat ccaccttcag tttgatctcc tggttgatat
     9061 ccttctgtac tttttccaga ttgcccgcca taccgttgaa atactctgtc aaagctccgg
     9121 catagcctac gaactgggat ttggctgtgg cactgttggg atcttttaaa agagcctgca
     9181 taccggtgat catcagctga tcgaagatag tcttgaatcc ggtactcttg ccgtcatcat
     9241 caaaatacgt ctccagctgc tgcatgtaat actgcttcat atcgtattcg cccagctgtg
     9301 cattgttgtc ccagaatctt ccgtcataga actcatcgcg gacgcgctcg attgccagag
     9361 tctctacacc ggcaccggca cagccatagg tctggaacac ccgcagcgca ttggcggcct
     9421 gctgtgtcac ctgttgtctg ctgtagcctt ccgtctgtac attggcgata ttattggaag
     9481 tggtattcat ggcagcattg ctggccagaa gtcctgtata tgcaatattt aatccaaaaa
     9541 attgtgaggg catctcttac ctccttggtt cttacgcatt aaacacccag cgtggaaaga
     9601 atgtgttcca gcatctcgct gatcacgttg atataacggc tggatgcgtt gtatgcgttc
     9661 tggaacttga tcatattgga cagttcctcg tccgtggata caccgataac ctgttctctg
     9721 gcgctctgtg tagcttccac ggtgctgacc tgattctcat aaatactccg gaatacatag
     9781 ccggaattgg atacctgcga taccaggtcg gtataataat ctacaaaagt agtctttttc
     9841 tgtacattag gattcagcgt gtaggcttcc tcggtaaatg ccgctttcaa agcatctgcg
     9901 gtctccttat cctcggaacc gtctgccaaa cggaatccaa gcttggaagg ctccttcgtc
     9961 agcgccggat ttacctgaag attgccgatg gtatacaggc tctcagggct gtccggatct
    10021 tctttcttca tgacccagta gtccttaagt gatccgtccg cttccttcac acttactttc
    10081 tcatacccat ccgttgtaac cttggtgaac agctggatgg ggctgccatc ctccatgcgc
    10141 atgtaaccgt ccgggtctac cgtgcagtaa cgggcatttt ctaatttagt gccgtctgcc
    10201 aactccagat ctccggtctg tactcctgca gccttcgcca gaatatcgtt caccttcgtc
    10261 accacgttgt ggatcatctg gtcgaattct gcctggatgt tcatgacaac ggactgggat
    10321 accccgtcat attttccttc ggccagatcc gtgtaattgg ctctgtgatc tcctcttgcc
    10381 agaagcatgg ctttcagtcc gccgacatct gtgttcaggt cggaagaaat ctccacggag
    10441 agatcaaata cctctgcgcc gtcaatatta taatctctgg tgccgtcagc tctgacggtg
    10501 taggtggcat cctgcggcca gaacggagtg tagaagccgg tggcttcatc cgtcttcaga
    10561 gcgatctcat aacaggttcc gtcctttacg aaatccacgc cctcgatctg tacggataca
    10621 ctgccgtatc gatcttcgga aaaggtcatg ttggtcagct ctgccagttc atccagaatc
    10681 tgatttctcg cgtctctcag gtcgttggca tgttcaatgc cgccactctc aatggcacgg
    10741 atctgatcgt tcagcgttaa taactggttg ccgtatttat tgatcttatc tacattctgc
    10801 ttaatctgtg tgttcaggtt gttctggtag gagctcaatc cgctgtatac tgctccggct
    10861 ctctgtacga attccgccgc tctctgcacc agcatgccct gtgttacaga actgctggga
    10921 tccttggaaa gctcctgaat agcggtccag aagtctgtca tggtggtctg gaatgcctcg
    10981 ccgttcaatt ctcccagctg gctctctacc tcttccatga cttctgtgga cacttcatag
    11041 aacatactac gtccggattc cttgcggtat gtcttgtcca ggaaataatc tctgacctgt
    11101 tttacgcggg aatatgtgac acccagacct gtctgtttgt tggaaataga cttagggtct
    11161 gtcgataatg tgacataagc tctgttagac tgctgtacct gttgtctggt atagccggtg
    11221 gtgtccacat tagaaagatt atgcgctgtg gtattcagcg cattctgact tgtctgtaat
    11281 ccggacgaac ctatatataa actacccatt aatggcataa actttcacct ctgtatcagg
    11341 ggatcctcag atccccggga tccggtctta ctgcttggca tcgaatccat ggtcaatgac
    11401 ccccatggta ttgcctgcat tgtaagctcc ccggttgtaa ttcgctgtct cgggtgcagc
    11461 cttggatgcc tggaggagat tcatctcgaa ttccaccatc tccagcgcgc tgtttaacag
    11521 ttcactgttc tgctcgttca cgcgcttcag tccccggacc gctgcctgta atctgtcgtg
    11581 ggaatccgcc agtgcctgct gttccgcagg ccgtgcagat aacatagtga tcagattcgg
    11641 caatttcaag tcgttaacat ccctgttcaa tacatcggct atgtccgcag tcactcccgc
    11701 tctctgcttg tccagattgt ggatcctgct gaccagctcc tgctcctcgt ccgtgatctt
    11761 ttgtaactct gcaagatcac cgcctgcaat aatcggtgtc ttgcgctgtg ataactgaag
    11821 cagagcttcg tactccgtac tctctctgtc cagtacatct atcaggtttt ccattaaact
    11881 agccaaggaa ctacctcatt tcttcctgct tttgaagcag cttctccgca aagcttgcat
    11941 tatccactcc ataagtaccg ttctggattc ttgctttaat gggagccgtt aactcttccc
    12001 tgatgtcagg tgctgctgcc actgctgcct tagcggtctg gaaatccttg ccctggctgc
    12061 tgatctgcag tctgtccgtg tgtgacgctg tctgagtctt ctgagttctg ctgaccttct
    12121 gtgactgata cagctgctgt acctgtgtat acgcttcaat acgcatatcg actcctcctt
    12181 cccgaatgtc ttttgatgtt tcacaatatg tatcggacgg atttacaatt actttagccc
    12241 tcatggaaaa aaagtaacga tccggaccgc ctgcagggga acgtccgaat cgtcacaaaa
    12301 tctgtcaata tgcattttcg ttgcggatct catcgtctac ccgccgtacc cggtaggtct
    12361 tgtctcccac cgtcacggcg gtcaccgtat cgtcgtgggc atacaatcct tcttccacat
    12421 aagaaaattt ttccggtgtc ctgccatctt ccaggatctg gatcaaagcc tccaccctgg
    12481 ggccatgcag cggattgcat tctccgatgc aggtgatctc atccttcagc acgcagtcca
    12541 tggattctgt gctgacacca tcaaaagaca atatcataat atctcctgcc gccagattgc
    12601 tgccgaccct ttttcccgcc gcccggatcg cttccatggc gccaaaggcc tcattgtcgt
    12661 tttcgcaata gaccacattg atattgtcgt attttttcag gaaagcttcc atgacttcct
    12721 tccccttggc ctgggtaaat tctcccgata cctggtctaa cagatcccaa ccgtattttg
    12781 ccgcgccctg ctccaatcct ctggtacgtc cgatctgggc ggaagctccg atatttccct
    12841 gaatatttac aatatgaaga tcctccggtg caattccctt gctgatggaa aaggaattca
    12901 gccactccga cattttcagg ccttccagtt caaagtccga acccacgaag caggtgaaca
    12961 gactgtcatc ctccacatcc accttacggt ccacgatgac caccgggatc ccggcatcct
    13021 ttgcttccct gagcaccgtg tcccatccgg tctccgtcac cggtgccagc acgatgtagt
    13081 ccacctcctg ctggataaag gttcggatgg cccggatctg gttggcctgc ttctgctgac
    13141 catcctcgaa gatcaggtcg tacccggcct cttttgtaaa ggtacttttc atggactccg
    13201 tgttggcact gcgccagtcg gattccgctc ccacctggga aaatcccacc acaatacggt
    13261 ctgtctgtgt atcctctggt gttgcaatat cctccgcagt gctttgtctg tcgccgcatc
    13321 ctgcaagcag gattcccagc cccagcagca gacccgccat ccatcgtctt cctgttctgc
    13381 atttcttcat atctgtaatg tcatcctgtt ctgtcatact tcccgcccgg aaatcgctct
    13441 gtcattctct cattttccgg caggttcttt ctctgtatga aaatacgcca cagagctctc
    13501 cagttccccg gagatctgtt tcattttatc acagtttgcg cccactgtct gtatctgtgt
    13561 cagaatttcc tcaatgtttg cattcacctg ctgattgttg gcagcatttt cctcactgat
    13621 agcactgagg ctaccgatat tgcccagaat atcctctttc tgctgtgaca gacgtccggt
    13681 ctctccggcg atcttgcgga tctcttccgc agaatgtctg atactttgct ctaactgctg
    13741 atactgctct ctggttgcaa taatgtgatc ctgttcctcc cggatgatat cctttaccac
    13801 atccgcctgt tccacagtct caccggattt ctccataatg ccctgggcca gatttttgat
    13861 ctgctcggca ccggcagcgc tctgctccga cagtttgcgg atctcttccg ccaccacggc
    13921 aaagcctctg cccgcctctc cggctctggc ggcctcgatg gaggcattca gacttaacag
    13981 attggtctgt ccggaaatgg agaagatcag agctaccgcc tcatcgatct tttcgatata
    14041 atcgttggtc tcatgaatct tcgctgtaat ggtattcacg gattccacag aacgattgct
    14101 gccggtcatg atcttttcca tgccttcccc cgcctttttg ctgttgtcct gtatttcttc
    14161 cgaatccttc tgcagctgtc cggcactgac actgatgtca ttgaccgcct ctccgatctc
    14221 atccatacgg tcactgatat tctgcacatt ttccgccatt tcctccgctg cggaagacag
    14281 ctgctccatc gcctgactga tctgtccagc ctgttctgtg gtggcatcgc ttccctctgc
    14341 cacctgccgt acactgttta gaagttctcc tgatacctcc tgtacaccgc cgatgacctg
    14401 tgccagattc cgctgcatct gtgaggtatt ccgtaacaga ttacggatct cccggacaat
    14461 actgtcggat tcttttctgg cactcagatc accttccgcc agggcatcca ccctgctgcc
    14521 ggtctctttc atcttgcccg acagtgctct gctcaggaaa aagcagataa caccaaacag
    14581 gatcaccatg cccactgcgc agaccaggaa tacaaccttg atctcccaca gggcatcggt
    14641 gatctcattt ttccgttccg ctgcaaaggc caccgcagtg atgcgtccgt cattcatgat
    14701 cgggatataa taaccataat aagtatttcc ctcaatcact acatttttat cataatatcc
    14761 ctccgtccca ccggcggaca caagatcctg atccatgggg atttcacgga tacgatagtc
    14821 attttcattt ttaatggatg tggtagccgg aatgccgtct gcaagaatgg ccatctccag
    14881 gtcttcttcc ttcagactgt ccaggtactc ataataaccg ctggcattca ttgccgtgat
    14941 ctcattttcc ttacagtatt ttgccagatt atccgcagtt acatgcagtg tggtctgcac
    15001 actgccttcc agattcgttc tggtcagacg caaagatgtg gtactgagga tcactgttgc
    15061 caaaactacc ggtgccagtg ccagcagcat caacaacagg aacaggctgc atccctgctt
    15121 tttcagagtt ttctgctttt ttaccttcgt ttttttcata acttctaccc cctgatattc
    15181 ggtggattcc ttttactagc ggaaaaagag tgtgactgca caccagccac gctctttccg
    15241 ctcctcccta ttttcagctg cttttatttg ttctgcagca ttttcttttt tcttgcgatg
    15301 accacactct gtactaccag gaacagacac agcatagctg caatggtgat accggtccac
    15361 catgcctgat ccagacctgc actggatacg atattctgga tggtgctcag ggacaataca
    15421 ccgaagaagg ttccgatgat attgccgaca ccaccagtca gcatcgtacc gccgatgatg
    15481 gaggatgcaa tggcgttcat ctccatgccg ctggcatgag aagcagatcc gcttcctaca
    15541 tgcaggaagt acacataacc gccgatacct gccaacagtc cgcagagcag atgggacatg
    15601 aacttggttc tcttcacatt gatacctaac atcagcgcgc tctgattgtt accgcctacc
    15661 gcatagaagc tgcggcccat cttggtccag cgaagcatga taaacatcag aatgactact
    15721 aaaagcgcca caatcacgcc gatctccaca taagcattga cgtaattgcc cagtttgttc
    15781 acggagccca ttcctggaac gatcacacgg gtttccttca aagcagtgaa actttcgttg
    15841 gctacgttga aaggtttggt atttacgatc gttgtcatac ctctggcgaa gaacataccc
    15901 gccagggtca cgataaaggg ctggatatcc aggtatgcta ccaggaagcc ctgtacgata
    15961 ccgaaagcaa ggccaatggc tactgccagt aacatggata caaatacatt accgccgccg
    16021 aagtccagat atacggcgca gctcatgctg accagtgcca ccacaccgcc gacggaaata
    16081 tcgattccac cggtgatcat gactaaactc atgccgcagg acagaatgat cagtgctgcg
    16141 ttagcgttca gaatgttgaa aaatgtctga ggttttaaga agccttttcc ctggaacacg
    16201 atggctccga tatacattac gaagaatact acgatcgtga tggtcagcag cagattggta
    16261 tctgtcatat tttttaactt cttacccata tcagccaacc tcctttacag ccttgccctg
    16321 cttcagcttc ttgccgaaag cggacagcca ttctcttacc ttgggagtac ttaagactac
    16381 cagcaggata accactaccg ccttgtaagc aggaagtgca tcggactgta cgtcgaattt
    16441 gtacaaggtg gtggtcagga actggatgac ataagcaccc agaatggaag ctgccatgtt
    16501 gaacttacca ccgcttaaag agttacctcc taaagctact gccaggatgg catccatctc
    16561 gatatccttt gcaataacgg agtagttgat ggaggacaga cggcttactt tgatcagtgc
    16621 tgctaccgct acgcacagtc ccaggatcac aaaggtcagg aacttgataa atgtgggatt
    16681 cagtccgttt aacttggagg aattctcatt gataccaaca gactgggtgt aaagtcccag
    16741 tgttgtgaac ttcaatacca ggaagatcac cagtacacag accgctgcaa tgaagaacgg
    16801 tgtaggaatg ggaattcccg gaataaatcc accgaaatag ctgaaggtcg gatcacttac
    16861 aataggaagc tcgttgttgt tgatccatgc tgcaatggaa cgtcctgccg tatagaggat
    16921 cagggtcgct accatgggct ggatcttgaa atatgctacc agggcaccgt taaaggcacc
    16981 gaagagcatg gctaccacac aggctaccag aaatgctacg ataatggggg cctgcagggt
    17041 atcgggtctg gaattcgtac cacacagtac tcgtaaaatc acgctgcctg cgatggcaat
    17101 ggcagcgccc acgctgatat cctgtccgcc ggaagctgca gtgaccagtg tcatgccaat
    17161 ggctaaaata gccagttcgg aaccgtagtc caggatcgtc atcaggtaac cggacaatac
    17221 cggatctcct gcgctgttat atcccagtgt gatcttgaag aaactggggt ctgcgatcag
    17281 attgaatact gccaggatta tcagggccgc aatgggaatg aaaatctgct gccggaacaa
    17341 tccgctcagg aaatttttat tacctttttc tactttcttt tcattactca tgcttacttt
    17401 cacctccggc aatcgtactc ataatcttgg tctgggtcag atcttctccg gacaattctc
    17461 ctaccacctt gcggtctctc atgactacca gtcgggagca ggtacggagc atctcgtcgg
    17521 tctcggaaga gatgaaggtc acgctcatgc cttcacttgc cagcttcagt accagcttct
    17581 ggatatccac cttggtaccg acatcgatac ctctggtggg ctcatccaga atcagatatt
    17641 cgggatgggt cagaagccat ctggctaaga ttaccttctg ctgattaccg ccggacagcg
    17701 acttgatggg tgtctcggcc gatgccgtct tgatatccag cagtttgatg tattcgtctg
    17761 caaatttatt agcttccgcc ttggaaatag ggtggaagaa tccacgaagc acctgtaatg
    17821 ccaggataat attttctctt acggacaagt cacctacgat accgtcacgc ttacgatcct
    17881 ccggcagata gccgattcca caacgcatag catccagagg cttattgatc ttgacctgct
    17941 tgccgttctt tttcaccgta ccgccgatga cacggtcagc accgaaaata gcgcggacgc
    18001 actcgcttcg gccagatccc agcagtcccg taaatccatt gacctcgccc ttcttaatat
    18061 agaagtcaaa gggcttgatt ccggcatcgc tgcacagatc tttggtctca aataagggtt
    18121 ctccgttctc cgtcttctgt tccgtgggct gctcgccttt gatatcggac aggtcatcca
    18181 gttccttacc caacattttg gataccagtt gtactctggg cagatccttg atggcatatt
    18241 cacctaccag actgccgtca cgcataacag tgatcttgtc acacacttca tatacctgat
    18301 ccaggaaatg ggttacgaag atgataccca cgcctctggc cttcagatcg cgcatcaggc
    18361 cgaagagctt tgtcacttcc tgctcatcca gagaggaggt cggctcatcc aggatcagta
    18421 ctttacaatc catatctaca gctctggcaa tggctaccat ctgctgtacg gcgatggaac
    18481 agcttgccag ctgctgggac gctttcgcgg ggatatccag attctgtaag atcttgtctg
    18541 cgtcctcatt catttttttc cagttctgat agatcttatt ggaacgtcct atgtacatgt
    18601 tctccgccac ggacagattc ggacagagtg taatctcctg atataccgta ctgatgccca
    18661 gcttctgtgc ctcctgcgga gagtgaatgg atacggcttt gtcattgccg tcgatgaaga
    18721 tctggccttc atctttgctg tagacacctg tcagtacctt gatcagggtt gactttccgg
    18781 caccgttctc tcccatcagc gcatgaatct cacctttgtt cagatggaag tccacacctt
    18841 tcagagcacg tacaccggga aagcttttgt gaatattttt catttccaat actgtgttat
    18901 tgtccaaaag tttcacccct taatctgtaa ctattcagag ccatgtaaat atcatgaaat
    18961 gttcttcgaa tgctggcatt cgtaagaata ttttatgata tttatagggc gaagtaacca
    19021 cattcgcaat aaagtgggct ctgaaagagc ttttgcaaat ggggttctga atagttactc
    19081 ttttgataaa agaagcgcgg agctagcatg cgccgcgctt ctggtcgttt acgtcagatt
    19141 agataccgta gttgtctaca tcttcctgag taatggtggt tgcatcgaag cctttctcat
    19201 ccatgatgat ggtcttctcg gagatggtct cgccgttctc cagcttcttg atgatttcat
    19261 cgatgtagga agcctggaaa ggattgcact gtccatcgta gttccagttg cctgccttca
    19321 gttcttccag agcccacttg ttgcagtcaa agcccataac gataacgtct ttaccaacgc
    19381 cgtgggagat attagcctta tccagagctg ctacagcacc cttagccata tcatcgttct
    19441 cagcgtagat tacgttgaaa ggagtacctg cgtcgattac ggactgtacg atctgctgtg
    19501 ctttctctgc gttccactca gcggtctgct gtgttaccag attgaatccg tcgtttgctg
    19561 ctgcgtcatc cagagcaccg gtacgtccct gctgtgctgc ggaacccata acaccctgta
    19621 agtggataat gttgtattca cttaagttct ggctctttaa ccagtcaact gcggtctggc
    19681 cttcctttgc catatcggat acgatggatg cctcgtacag gctctcatct gcatcaatgg
    19741 tacggtcgaa gaggataact ctgatacctg catcctgtgc atccttcaat acggaatccc
    19801 aacctgcggt atctgctgcg gacagaagca gatagtcaac gccatcctga atgaacttct
    19861 gtgctgcagt gatctgctca tcgttcttta agctgtatgc gaaagatgct tcgtagccgt
    19921 tagcctcggt aaaggtattc ttcatatcct tatcgtttgc ggtacggtaa ccggactcgt
    19981 tggggtcgtt gttgatgatg ccgactttga tcatatctcc ggaggagctg gagctggtcg
    20041 ttcctgcgct tgcactttcg gttcctgcgt tttcacttcc ggttgttgcg cttgcacttc
    20101 cggttgctgc gttgctgcct gcatcagagg agccgcatgc tacaaggctg aataccatgg
    20161 ttgctgccat tactgctgct aaaattttct tcttcattgt gtaacctccc aatttgtttt
    20221 tgttccccaa agaactattt ctttgatgat cttattgtaa agcggatccg gggaaaaaac
    20281 catgaagata cctctcggaa aagttgaaat ttttatggta aaaaaaacca ccggcgtaaa
    20341 aagttgattt ttttacgata gtggttcatt tttttaatac tgtggaagcc ggaccgtgac
    20401 acaggtgccc tccccgtagg tactggtaat ggtgatacca tagttttcgc cgaaattcag
    20461 acggatacgt tcgttcacat tgtacaggcc gtagatatcg gtctcccgga ccttccggtt
    20521 gcggatgcct ttcctcacct gctccagccg ctccggcgtc atccccttgc cgttgtccgt
    20581 gatcaacaga atgcaatcct ctccgtccag ttctccttcg atccgtatca tgcccttgcc
    20641 acgtttgttc ttgattccgt ggtacagtgc attttccacc agaggctgca gcgtgatctt
    20701 gggaatctgg caatggttta attcttccgg aacacagatc tcatattgaa ggatgtcctg
    20761 ataacgcatc tgctggatct ccaggtagct gcggacgtgc tgcagttctt cttttacatc
    20821 cagaatatcc tgtccctgat tcaatgagat ccggaaaaag tccgacaggc tgcctaccat
    20881 gctcacgacc tttttctgtt ctcccgcctc cgccagccat acaatggcat ccagcgtatt
    20941 atataaaaag tgcggattga tctgggactg cagcagttca aattccgcct tgcgcaaccg
    21001 gatctgctcc gtcttcacct gctctaacag ttcgttgatc ttgtcgatca tggtattcag
    21061 tgaatcgctg agcacctggg cctccacgcc ccccgtcacg tcggagcgca ccgtcaggtc
    21121 accttttgcc acctgatccg tcacctgggt aaggcgtctt atggggctcg tgatacttaa
    21181 cggtatataa taggataaga ataaaaacag aaccaggatc agtgtaaagg caatgatgga
    21241 gaaacggatc atattcacga agaaatcctg atattgctgc ctgctctcct gaatcccccg
    21301 gatctcgtaa tagatgtact gggacatggt atcacccacc agagacgtca caatctgcac
    21361 atcattttcc cagatttcca tgttgtcctc gtagcggttg ccttcccgga tattgtcctc
    21421 gatcctgccg atataggtct ccagattgtt cagatatttt ttgacactgg tcagtcgttt
    21481 caggttctcc tgagattccg tcagttcttc cagaccggcc acgatgtcat ctgcctccac
    21541 cagcatatca tgcagactgg actcttccag cgtcttgttc cctacgatca gcagataagt
    21601 ctcataatcg aaatccttct ggaaatccag gctgaactga ctggccatga cggaggaatt
    21661 gatcatatcc tcgtatctgc ggttattgtt ccataaatta tagaagcaaa agatcaggaa
    21721 caatacaaaa ggcaccagaa ggcaaagata ggaatatctt atctttgcca ccagtgtcga
    21781 atgttcatac aattctttta tcttttttct cataggaatc ccccttatcc atggccacgg
    21841 ctaactggtg ccgccccggt actgggtagg ggtcattccc tgggtcttct tgaacagata
    21901 ggagaaatag tgtggatcct tgtagcccac ctcctggctg atcaggctgc tgcgcttgcc
    21961 ggtgcagcgc aagagttcct ttgccttgtt catacgatag tctgtcagat atttgataaa
    22021 gctctgtccg gtctgctggc tgaagatcat gctcagatgg tttggtgaga aattcacgtg
    22081 ggatgccagc agattcaggg aaagttcctc gtctgcataa ttctcatcaa tgtatttcat
    22141 cacctgttcc accacatcat cataccggct gctggcagcc ttatctctga ctgccagtgc
    22201 tttctgcaga attctggtaa tgtaatggat cgcactatcg ctgtcctgca gggtcttctc
    22261 gtcctgattg ggtgtttcca gttcctgcgt atccgtctgc aggctttcca gaaaatctgc
    22321 cacacagaaa tagacatcca tggtaatata ctgcctgaaa atattggact gcagtgcctt
    22381 gttgctgaga tcccggaaaa attcttccac gaagtagacc acttcctccc ggtcaccctg
    22441 ttttaagaat tcctggattc ttgtgcggtc gatctgcttt ggattgactt ccttgatgtc
    22501 aaaattatct tccttctgcg cttcctccgt catatgtccg ctgtccagga tccggctgtc
    22561 ctgcaccaga tatctgtggg caaaagcatg actggcctgt tcaaaagatg tcggaagctc
    22621 ccgcagacgg ttcaccggct tgccgatacc accgaaatac cgcacatgct catagccttc
    22681 cataatgtcc ttgatctttt ctatgtattc ctgttgcagc agcaatagct gttcctcgga
    22741 atcggctttt aataagaatg cttttccttc cagattacga tcaaaggtga tcaccgtcct
    22801 gccgtcttcc agtccccgca gttcctgttc gatccggatc aggctgccgg aatactcatc
    22861 ctgcgcatga cgcagagact gggtcttgaa cagaataata ttgtaccagg gtgacgacag
    22921 atcgatcccc agaatctctg ccgattccaa cagttctgcc acggtgcttt ctccggtgac
    22981 cagttcctgg aacagttttt tccgctctct gagattgttt tcctccatct cctgcaaata
    23041 ccgctctctg atctgaatgg ctatgcgctt ctcctcgatc ttcgccgaca attcatccaa
    23101 ttccgccagc agttcctcac cattgatggg tttggacaga tactgggcca ctcccagctt
    23161 gatagcttcc ttagcatatt cgaattcctg gtaaccggac agaataatga tttctgtgtc
    23221 cggaagctcc ttgcggatca gacggctcag ttccaggccg tccataaacg gcatccggat
    23281 atcggtgatc acgatatccg gacgcaattt ctggatcatg ggataggcca gttctccgtc
    23341 accggcctcc ccacagaact catagccatg agccttccag tcaatattat ttttgattcc
    23401 ttcacggatg acaaattcat cctccaccaa aaacactttt aacatttcca cgataccctt
    23461 cttctatctg caggtaactt ctacccgcag attctgcctg cagattttta tttcgttctc
    23521 tgcaatacta ttttctatta tacacgaaaa cagatttttc gggtagcata ttttagcggt
    23581 agaatacttc tcccagcatc aggtctctct tcagatcgcg gatcttcgta tccttgtcga
    23641 tgtatacagc ctcgatgccc atcatggctg cccagtcacc catctgctct gcggtcagat
    23701 cgtaggtaaa tgcggtatga tgtgcacctc ctgccaggat ccatgcttcc gcgccggtat
    23761 acagatccgg ttcgggagtc cagaagttgg tagcggtagg aagcttcggc atgggtttct
    23821 ccactttctt gcagttcaca tcgttgatga tcagacggaa acgattgccc atgtcgatca
    23881 gggaagtagc gataccatga ccttccttgg aagtaaatac cagtcttgca ggatcttctc
    23941 tgtctcccat ggacaggggc tggcacttga tgctgatggg accgtccgca atggtggggc
    24001 agatctccag catatgggct tccagaatac cttccttgcc cttgatcaga ttgtaggtgt
    24061 aatcctccag catggaagta cccttggcat cctttttgcc tgcggtcatg atcttcatca
    24121 gacgtaccat ggcggctacc ttccagtcac cttctgcgcc gaagccgtag cccttctcca
    24181 tcagtctctg aatggccagt ccgggcagtt gcttcaacgc acccagatct ccgaagtggg
    24241 taacgatggc ctgataattc ttctcttcca ggaaacgttc aaatcccagt tcgatctgtg
    24301 cctgtacagc cacatgtttt ttaaactctt cggggtctct tccctctaat aaaatattat
    24361 atttctcgta gtactcgtct accagtgcat tgacatcgga tacagatacg gcatttacac
    24421 tctctgcgat ctcgtttaca gggtaagcgt ctacttccca gccaaacttg atctgtgctt
    24481 ctaccttatc accttcggtt accgctacat tgcgcatgtt gtcagccaca cgcatgacac
    24541 ggatatggct gctctccacc acgccgactg cagtacgcat ccaggatgcg atcttgccct
    24601 gtaccacagg atcagaccag tggcctacca caaccttacg ctccatgcgc atgcgggtca
    24661 cgatatgacc gtactctctg tcaccgtggg cagactggtt ctcattcatg aaatccatgt
    24721 cgatggtgtc atagggcagt tcctcattga actgagtatg gaaatgcagc aggggcttac
    24781 gatactcctg taatcccagg atccaggatt ttgccggaga gaaagtatgc atccaggtga
    24841 taacaccggc acaagtgtca tccagatttg cttcattaaa agtacggcgg atcagttcgt
    24901 tagtgatcag ggtcggcttc cataccacct cataaggtaa cacaccggaa gcgttcaggg
    24961 catccacgat cttgtgagag tgctccgcta cctttgccag gcactcgtcc ccatacaagt
    25021 cctgtgatcc cgtacaaaac cagaattgat atttttttgt ctgtaacata agtaatacct
    25081 cctgtagttg aatacaatga tgtcggggtc aaaagccccc gactttgatg cctacggatt
    25141 gttccgtgct acgcactccc acaatcctac ccaatattcg catatcgtgg gattatcatc
    25201 ccacttttat gctcatatcg gggcgggtcc taaggtcttt cacccaccta tgagcaagct
    25261 catgtgggct tagacctttt gaccctggca tcatacaatt tcgatattac catattacaa
    25321 cttgtatgct cctgctaaca agttgtattt cctacgacaa gctccggctg gatgagaatc
    25381 tttctctctt ccggtcccag agattcccca tgaagcagtg acagtaacag ctgtgccgcc
    25441 atttctccca gtttttcctg gggatgaacg atagtggtca gttgaaaacc tccgccgatg
    25501 gccatgtagg aattgtcata accggtgacg gaaatgtcct ccgggacgcg aatcccccgg
    25561 gccgtcagcg tcgggatgac ctgcatggcg atctggtcat tgtaacagac cacactgtcg
    25621 agtctggttc ccttggagat cagacgccgg attccctcct gtgggtggac tttacgatcc
    25681 tcggtatgga accagatcac cttgtccgga tcgtagagga cacctgcttc ctgcagggcc
    25741 tgtacatagc cccggtgacg gttctgtccc tggatgtcat ctgccttgaa cactccggca
    25801 atatcgcgat gcccggtatc caggagatat tttgtgatca gatgacctcc cctgcaatcg
    25861 tccatcagca cctggggctt gtcttccatc tggtcgaagc agccttgaat aaacacatag
    25921 ggaattccgt attcttccag ccgctcatac agatgcagat gtctgcagct gatctggctc
    25981 ttactgggtt cgatgatgac cccgtcgata tccttctgca acagttcctc cagacaccgt
    26041 gcctcctggc tccgggagtt tcttgtattt tttaataaaa tactataccc ctgtgccgta
    26101 agaacttcat cgatgccctg aataacccgg ggaaaaatat aatccgacag ataggtggtc
    26161 accacggcaa tgttgtgaga cttatgctcc gggcggaccc gctccgacac aaaggtgcct
    26221 ttcccgtgta ccgcatagat gtatccctcc tgttccagaa tggccagtgc cttgcggacg
    26281 gtctggcggc tcacctgata agcggcggcc agctggttct ccgagggaac acggtcaccg
    26341 ctgcgcagtt ctcccagtcg gatctgttcc tgtaaatcat gatgcatttt atagtatttc
    26401 agctgttttt cttccatacg tatccgtccg ccccgctcct tctctatccg gttttgtaca
    26461 actctattcc gttttccggg tatcgctccc cggtacattt cagtattttt cgccttccag
    26521 caggatcagc cgccggattt cttccgctcc gctgacacgt tccccttcta tggtatcatt
    26581 atggatcaca taatcacaga tcttgcgatt acgcttctcc gcatcgatag acaggatccg
    26641 aagtgtcttt tcctcggtgt catagtcgga atcaatgata tcggcgatca gcttttcttt
    26701 atctctcgcc acatagataa tatgggtggg aaaggaacgc ttcatggcaa tggcaccgca
    26761 catatccacg gggatcaccg caaactttcc ctgatccagc aaggtctgaa tgtcttcctt
    26821 tctggtgccg taccgtaccc cggcataagc cgttttttca aaaaagttca tccggtcgaa
    26881 ggcttcctcc gggacgtact tgtggccgta gcgccccggt tccgtgcagt agttcaccgg
    26941 gcggacaaaa atctccccac tctctgtcct ttcgcttgtg cctgtcccgc ggctaccgca
    27001 caaagcttcc gtaatctccc gtttgccgga gccggacggc cccaccaggg ccagcaccgc
    27061 cggagcagtg atcttctctg tcttagaagt ggatgctttc atgatctgct tcaccagaga
    27121 gacaaattcc gccatggtat ttacggacaa cagccccgtc attttggcat tccagggctt
    27181 ccgcatcagc accggatact ccgccttgct ctccagaata ttgtggatgg catcatccag
    27241 cacaatatca aaatggacac gatccttggc acttcccaga ataatatttt ccggtggaag
    27301 ctccgggaac tgggtcatga tctgttctgc ccgcactccc ataaaatgtg gtgacaccgc
    27361 agtaatgaaa aaaacatccg cgatctgcat cagtctgcgg atccctcgtt tcgtctcctc
    27421 ggtcgggatc tgcctccggt acagttccgg atcgttatag aattctttga tcaccgaagt
    27481 ccgcccttcg ttggcccagg aagtgatttc ctccatgcga taaggttcct tcggatgata
    27541 tttttcattt gccatacgga tggcgatact gttaaacggg gcggtaacat cgtcccaatc
    27601 aataccgatg ctgggtctga gcatgctgta gtcctcactc tttctatgtt caatgcgatc
    27661 tatcacacca tgtgatttta gtgcattatg ctttttgcac attgtgcatt cccgtttccg
    27721 gaatacttct gctatttagc atagcataaa cgaatacgca gtacaatcca taccgcagcc
    27781 gctctctctg gctcttctac cgttccaatc ggcattgttt attttgccgt actctttttc
    27841 cttggatata tcatgttttt ggggcttatc atccatttat catatttttt gttgtaataa
    27901 tgcacaatcg cttttttctc tatattgccc aattgtcagc acttttgaac tgtgctagta
    27961 tacatgtaca ggaggagaat tcatgaccaa ccgggaatta ttagagacga tagtaattaa
    28021 attagaaagt atagaccaac gactgggcag ggtggagcaa cgacttgata aggtcgaaca
    28081 gcgactcgac agggtggagc agcgacttga taaggtcgaa cagcgactcg acagggtgga
    28141 gcagcgactt gattcactgg agcaaagatt cgattctttg gaacaacgtg tttccaatct
    28201 ggaatccggt cagattgcca tggaacagcg ccttaccaat ctggaatccg gtcagattgc
    28261 catggaacag tgccttgcca atctggaatc cggtcagctc tctatggcga atcgccttac
    28321 caatatggag ttgactcttg agaatgaagt cagtcgtaat atctgtacaa ttgcagaagc
    28381 tcatttagat ttatccagaa aattcgaccg ggccttaaaa gtaaaagaag atgatgaact
    28441 ggttcgcgtc cggcttaatg tattggaaaa tgatgtccga aaaataaagc agcggctgga
    28501 aattgcatag tttattctta tcagtacctt atcttaatgg ataaggtatt tttttgccct
    28561 tctttttgtt gacttatcaa catccttgtg ttacacttca taaagttgtt gcagtgtcaa
    28621 cattttacag gaggaacctt catgcaggat cgttttgaaa gatttgtggt atctatcaca
    28681 gaattgcacc gttatctgca aaaattaaaa gaactggaaa tgggacagat ggacttaaaa
    28741 gccggttata ccatgtgtct gtattatctg ggaaagcacc cggaagggct gaccgccacg
    28801 cagctgacgg agctgtgtaa ggaagacaag gccgccatct cccgtacctt aagccagttg
    28861 agttccaagg ggctggtgtc ctgcgagctg ccggaacata aacgttccta ccgcacactg
    28921 tattatctga cagaagaagg caatactgta gtcaaaaaga tcagttcccg gatctattcc
    28981 gcactggctc acggcggtga tggcatgacg gaagaacaac ggttgaatct ctatgactct
    29041 ctggaacgga tctcccataa tctggagcag tatcttacag agttttctgt caagcatccc
    29101 acagagaaat gatccatccg caactcaccc tgaccggctt tccaacagaa ttaacagcaa
    29161 ggtgacatga acgatgtcgt atgtccatcc cgatggatgc cccggcgcat ttccgcccga
    29221 aagaatattc atcataacta tatatagaaa gtacaggaaa caattatgga aaaaatacgt
    29281 tttatcacgg actccgcatc cgatatgaac aattcaagag aggacgtcac gattctcccc
    29341 ctgcatatcc gcttcggtga ggaggaatat gctgacggtg tcaccatcag tcataaggaa
    29401 ttctatgaga aactgatcga atgtgatact ctgcccacca ccagtctggt atctcccgca
    29461 gtcttcgaag aagcctacga aaaagctgta tcggccggag aaactgtcat tgtggttact
    29521 atttccagca agctgtccgg cacctgccag agtgcccgga tcgctgccga ggattatgaa
    29581 ggcaaagtct tcgtcgtaga cagtctgaat gccaccattg gggaacagat tctggtagaa
    29641 tacggcctgc agctgaaaaa gcagggaatg accgcggaag agatcgtaag cattctgcaa
    29701 gagcaaaagc ataagatcca caccatggga ctcttagaca ctctggaata cttgaaaaaa
    29761 gggggccgta tctcccccac tgtggcattc atcgggggtg ctctggccat caagcccgtg
    29821 gtagcagtcg tagacggtga gatcattatg ctgggaaaag cccgtggttc caaaaacggc
    29881 agtaacttcc tcatccgtga aattgaaaaa gcggacggtg tggacttctc caagcccttc
    29941 tgtctgggct ataccggtct gagtgacgaa cttttacaga aatacattca cgacagtgag
    30001 catctctgga aagattatgc caaggagctt cccgtcagca ctctgggtgc caccatcgga
    30061 acccatgtgg gcccgggtgc tgttgccgtt gcattcttcg aacattaaat ctattacaat
    30121 ttttcccatt tgctcaaaat aggactaatt tcccacagct gccccactga caaatttctc
    30181 ctcttatggt atattgtaaa atcattttat attgtttgtt cattttctac attaccagag
    30241 aggagaattt ttatggggtt gaacctgtta ctatccacaa atcatatcgt catccacagt
    30301 tactctcccg tggcagattt tctgttggtg gttatgtgtc ttctggcatt gttactgatc
    30361 cgacagactt tcattaaaca gagccggacc tttcggatct tcaagtcctg ttttgccttg
    30421 cttttcattg ccgcagtttc caatattgcc ttttattatg ccgacctttt ttccagacag
    30481 aacaccctac tctggatcct ccgggccgtc taccatatca gtcttctgtc cctgttctgt
    30541 ctctatattg cttacctgaa aatactgatc gtcttcccga aaaatatcgg caggaaatat
    30601 acctttctgt ccttcgccat ccttgtggtg accggagttg ccgatctggc atctcctctg
    30661 accggctggg gatttcatca ggatcattac ggaatctggt atgaaaatat cctgtccact
    30721 ccttttatgg tcggatatct tctgtatctg gcggtgatcc tctttctgtt ggtttgctac
    30781 cgccgcagac ttcctaccgc actgttccat atgctgatat ttactgagac ggtctgtgga
    30841 ttgattgtag tcatggaagc tgccatgaat acgacttctt tcctggccac aacctatttt
    30901 ctgccgcttc tggtggtatt gtacatgctc catgccaacg cctacgaccc gaagaccggt
    30961 gccctgggtt ccgcatcctt ggatgaatat ctgcgccagc agcggcagac cgctcaggat
    31021 acctactacc tgtgcctgcg ctttgacatg gatttcgaat acgtcatgac agaggaaata
    31081 ggaaagctgt tctacagctt ctggacggat tattttcgaa agggtatgtt gttcaatcct
    31141 tccaccagct tcttcgtact ggcagtggac agccacaata tcccggatgc cacagaaagg
    31201 gcagtatccc tgattaaaaa ggtgttccag aaatattatg aagaatataa gcttccctac
    31261 aagctggtac tcttcgacca tctggacttt tgtgagaatc tggaacagtt ctacgaagta
    31321 ttcaattact tttccgaaaa agtagcgcag aattcctatc gtgtcttcgg ggaagaagat
    31381 taccagactt ataaagaaat gcattatatc aagtcccagt tgaaggatat tgcggaacac
    31441 ggctccttag atgacgaacg cgttctggtc tattgtcagc cggtacggaa tgtccacacg
    31501 ggtacttacg ataccgcaga atccctgatg cgtttgcgtc tgccacagac aggactggta
    31561 ttcccggatc gttttattcc tctggcagaa aaatacggct acatccaccg cctcagcatg
    31621 atcattctga ataagacctg taggcagatc aagcacatgc aagatgaggg ctatcagatc
    31681 tcccgtgtat ccgtaaatct ttctgtggaa gagctgggcg aaaaggactt catggaagaa
    31741 ttcaaatcca tcgtacgagc cgccggaatc gacttccaca ccattgccgt tgagtttacg
    31801 gagtcccaga acgatacgga atacgaactg gtacaggaat gcgtcagtga gttcaaacag
    31861 ttgggggtat gtacttatct ggatgatttt ggaaccggtt attccaattt cgaccggatc
    31921 ttaagcctga aactggatgt ggtcaaattc gacagaagcc ttttactgat ggcggatcag
    31981 gatgccaatt cccgcttcat gctggattac ttctctaccg cctttgagcg tctgggttac
    32041 aaggtactgt acgaaggtgt agagaccgat gcccaggaag aactgtgtat catcagtcat
    32101 gcagattatt tacagggcta taagttctcc aagccgattc ccattgaaga attgccccgg
    32161 ttccttacgc ggggctgata ttgcgattgc cgcatccatt attattccgg acaccgcttc
    32221 tgcggtcccc gcatacacaa taaaaatccc gaaggccttt cacagtctcc gggatttctc
    32281 tttgcttaac tttatttatc ttttgtatct tgcgcttata ttaagcagcc ttcttagcct
    32341 tcttctcttt catctgctta gcgaaggtct gtgcgccttc cactaccagg aagattgcca
    32401 gcactgccat tgcagttgcg aagaacagct ggaaccagtc accccatgca gcggtgccgg
    32461 cgccgatagc cttacacttg ttgattacag tgatcaccag gctggtcagg gttgcagcaa
    32521 gcatgaatac catagggaag tagaacatct tgttgttctt accaacttca cccagccatg
    32581 cagctactgc catcagtgcg atacctgcca gcaactggtt ggctgcaccg aacagacccc
    32641 agatctggct cagagacaga ccacccagga tacaaccgat cagtaccata gaaacagtac
    32701 cgaacagagg atgagccagc actttacgtg cgcccttggc atccttgtag gtagcctcgc
    32761 cttccttcag gaacagctcg gagaacatga aacggctcag acgggtaccg gtatccagac
    32821 tggtcagcgc gaatacggat acagacagag ttaatagtgc gtaaatggtg ccatatgcct
    32881 tggaagtctc ggtaccgaac atggatgcga tacctgcagc gaatgcagct gcgggagatc
    32941 cgaactcacc gtttaaatac ttctgatata ctgcaccgac tgctaccaga gcgatgatac
    33001 ccagagcaga ctcgatcagc atggagccgt aaccgattgc cttggcatcc ttctcgttag
    33061 ctaactgctt ggaagaagta ccggtagcga tcaggctgtg gaaaccggaa catgcaccac
    33121 atgctacagt gatgaacagc atagggaaca gggtctgtcc tgccttgggc tcccagctag
    33181 tgaaagctgg aatatcgaaa gcaacagtac cggtgaaagc acctgcaacc acaccgatga
    33241 atgccagacc gatcatagcg tacagcagga agctggacag atagtcacgg ggctgtaaca
    33301 ggatccatac gggaaccagg gaagctacag cgatgtacac acctacgaat acgatccatg
    33361 cgatacggtt cattgcaaaa ccaacgttca agccaagaac agtgatcagt acgataccga
    33421 tgatgccacc gatggtagcg ggcagagtct tgacacccat tctgttggtc acataaccat
    33481 aaataacagc cagaacgata aataacaaag agatcatagc ggtagactgg ttaccggtag
    33541 gattggtcac aaaaccgaaa gcggtcttgc cggcttcgtc tgccacagtc aggaaagtac
    33601 ctgccacgat gttaacgaag gaagcgatta ccagaatcaa taccagaagt gcgaagatga
    33661 tgaaaagctt ctttgctctg gatcccatgg aatcctcgat gatctcacct acagatttac
    33721 cgtcatgtct gatagatgcg aataaagatc cgaagtcctg cagaccgccg aagaaaatac
    33781 caccgataat acaccacagg aatacgggaa cccatccgaa gatggaagcc tggatcggtc
    33841 cgttgatcgg gcctgctccg gcgatagagg aaaaatgatg tcccataagt acggcgggct
    33901 ttgctgcaac atagtctaca ccatcctcct tctcaatagc aggagtcttc ttggtaggat
    33961 caatgcccca ctgttttgcc agccaggagc catagaacac atagccggta aacaataata
    34021 cgatagcggc caaaacaatt aataatgctg tcatttgtgt ttctcctttt cttatacaca
    34081 gatttaattt ttcgcaaata cacgaattgc tccactaaaa tccgctctcg caattctaaa
    34141 actgttcgga atccgttaat ttactgcttc ctcacagttt ttgggtccat aaattcaaat
    34201 atcttaccat gcaagcatgc acgatatttg gaatttctgt cccttgtatt tactgtattg
    34261 cctttttcac gagcttcttc agaatctgtc cgtggcggtt gtggacgatc cgacccgaag
    34321 ataactcggc gacaatcaaa cggtaattac tccagccatg aaatccccag aggtaactct
    34381 gataatgctt gcactttttc acctgtgtaa gcgcatcctc ctctgcatga ccggtcagaa
    34441 tgatcaggac aacatcgctg ttccgatgat tggatgaggg ctgcacgttg gcagttcccc
    34501 gttcccaggc gatggagtcc agttccagaa ggcgatcctt tgataaagat tcctctgtgg
    34561 caaaatacac agtctcgttg gtgtcaatat ctgccacctt agctttcttg atcagaaagt
    34621 actgctcatt atgggatccg aagattgctt cggctgcgaa tggcggttct acagcctctg
    34681 ttttgatatt gtagtaggtc tgataagatc gcaggacgcg ttccagcgct tccccgggag
    34741 taagtgtctt ttgtaattcc attctgcttt ccttttaagc atgcgtaatc gattacattt
    34801 acgttcatgc tatcgatagc tttagcacgt cgaagtgtca aatgcaacgg attttttagc
    34861 atttctttac acttttttat aaatacatac tttatcgtta tatgttagtc ctctgacagc
    34921 ttttttccaa ggtgcaaatt taacctatat accattacaa atttaatctc tttatctgga
    34981 taaagttatt gacttttgtt gggtaaccgg aaatcattct aaaaaaagca gccgattttc
    35041 atcagctgcc gatttttagg aatgattctt ctcttccttg atctcccaat ggatcaggaa
    35101 agtcagtgcc gcacgaagtc cgatgatacc ggctacggta acgatctcct tccagtctct
    35161 gacaataacg gtcttaagga tctcactgca caacttgaat tccagcccta cagcaagccc
    35221 ctgcgccagg gtgatcttca tatccggcac ccgccgcaca taattataaa accctttcag
    35281 accggagaga ataataacga tcactccgac aaattcaaag aacagtattg ccacctggac
    35341 aatattgttc atcacgtttt ccaggaattc aatgataagc tctatgtgga acatgcacag
    35401 ccccccgctt ttcttacact tcttttttca ttgtagcgta tccaaaaagg gcgtgcaaga
    35461 tgtatcttta tctttttatc agctattttt cttctgtttt caataattcc tgcatagtat
    35521 gccagccaag caccgcgcat tttaccctgg caggcatgtg ggcgatatcc tgcaatacgg
    35581 aggcttcctc cagttcgtcc cgctcttcct cggtaatact tcccttgatc atcttgagga
    35641 acaggtctgc cagatgcaac gcttcctcct tgcttctgcc aatgaccagc tccagcatca
    35701 tgtccgcgga agcctgggaa atggcacagc cgtcgccttc gaaggcaccg tcaactacca
    35761 caccatcctc cactttcagc tccagaaaaa tgtcatcacc acagctggga ttaacgccct
    35821 ccaacaccag atttgcatcc tccagcttgt gcttgtgata cggatgcaga ttgtgctccg
    35881 tcaggatttc attataaaat gtcttattct ccatagccca tctgtctcct tatctttgca
    35941 aggctttcta aaaatgcctg aatctcttct tccgtattgt agacacccag gcttactctg
    36001 gtggtggaca atactttcaa atgttgcaat aacggctggg cacaatgatg tcccgcccgg
    36061 accgcgatat gatccgcatc caaaatagaa gccacatcat ggggatgcac accgtccagt
    36121 ttgaaggtca caatgccgtg atggtcttct gctttctgtg agcccagaat gtgtactgcg
    36181 ggaatctctt tcatgccatc catcagcatg gctgtcagct tcgcttctct ctcttcgata
    36241 aagtcaaaac cgatcttctg cacataccgg attgcctctg ccagtgcata ggctccggca
    36301 ccgtttaccg ttcctgcttc gaacttatgt ggcacttccg cccacacggc tccggtacgg
    36361 ctgacggaat cgatcatttc cccgccgcac aggaaaggag gcatctgctc caacagttct
    36421 ctcttcgcat ataacgcacc aattcccatg ggagcataca tcttgtgccc ggaaaatgcc
    36481 aaaaaatcca catccagatc ctgtacatcc actgccatat ggggaacgct ctgggctccg
    36541 tctgccacga aataagcgcc tgcctcatgt gccagctgcg caaaacgctt gatatcattt
    36601 ttgcagccca gcacattgga tacctgggtc atagcaacga tcttcgttcg ttcgctcagt
    36661 gcctccttaa aagcttcctc ggtatatttt ccgtcttcgg tgcactccag atacttcacc
    36721 actgctccgg ttctggctgc cgcctgctgc cagggcagga gattgctgtg gtgctccata
    36781 atgctcacca ggatctcatc tcccggtttc agtaccagtg agccgaaact gtaggccacc
    36841 agattcaagc cttccgtggc attgcggacg aaaacgatct ccgccgcctc tctggcattt
    36901 aaaaactccc ggaccgtctc tctggcttct tcgtattttt cggttgccgc ctctcccagc
    36961 tcgtataatc cgcgcagggg attggcattg tatttttcat aaaatgttct ctctgcctcc
    37021 agaacacagg acggcttctg ggaagtcgcc gccgtgtcca gataaatcac atcgttactc
    37081 ataagcagag gaaaatcctt acggatctcg ctgattgaag tcgtcatact tcctcctgtt
    37141 atcttactct tccgcttcct cttcttcccc ggctctgcca aggaactcct gtaccttagc
    37201 ccgtgtagct gcatcaggaa tcttgcggca gaccgcatcc accttggcca tagccatcat
    37261 ctcatagatc tgctctctgt tcatgctgcg gctctccagg tagaacagca gctcatcgtc
    37321 cagtttgcca atggtggcgc cgtgattacc ttccacatct tcctccgcac agaggatcag
    37381 gggaatggtc tggttcacca catcgttgga taataacagt acatcctctt tttcattgcc
    37441 tactgcacct ttagcccccc atttgaaatc aatggtgcca cgatacagct tgaaagcatg
    37501 atcccgcaga acgccgccgg cctgcatatt gctctgggtc tttttgcctt catgcacagc
    37561 tacataattc atatccagtc ttccgtcacc gcccacgata taagcggtgt cggcattcag
    37621 gctgctctct ctgccctgta aggtagtctt gcatccaagg taagtattct ttccgccgag
    37681 gatcagctgg atcacttcca gggaagcctt ctcttcacac agggctccga tatcattcat
    37741 aaaagtaaaa tcactgccca gtctctgcac ctggatcagc cggagcttcg ctccttcttc
    37801 cagatacagt ctggtggata ctgccgcctg ccctgctgcc tccctgtcgg aagcaaaatc
    37861 ctggatcaca gtgatcacac tgtttttacc tgcttttacc gctacagctc ccgcctgtaa
    37921 gctattctgt gcataggcat aatcaagcag taacggctgc acctctgtca catccgcggg
    37981 cacctcatat tgttttgctt ttactcccgc tgcggcaagc agaccgtcca tatcttcccc
    38041 cataccggag gcaatgcctg caaaatccgc attccagggc ttagagcctt cgttttcagc
    38101 gatcgttatc ttctccggca gagtgacttt tgcctctccg gccgcgggga tctcaatttt
    38161 ctccaactgc gtctcgttca tgcgaagcca gttccaggtc ttcgccggca gtctgttaat
    38221 ggtcatatta tattcttttt ccatactgat ctccattcct cctgccacat taaccgatgc
    38281 tgcccttcat ctccagacgg atcagattgt tcatctccac cgcgtattcc aacggcagct
    38341 ccttcgatac attgtctgcg aatccgctga cgatcatggc tctggcatcc tcttctgaga
    38401 taccgcggga catcagatag aacactgctt catcgctgat ccggccgatc ttcgcctcgt
    38461 gtccgatatc ggcatcctgg gtacggatat ccatggccgg gatggtatcg gagcgggact
    38521 tgtcatccag catcagggac tgacaggata cggcggattt gctctttgcc gcactgttct
    38581 tcaccactac ggaacttcgg aaagtactga tacctccatc cttggagatg gacttggtat
    38641 tgatatagga agtggtctcc ggtgcattgt ggaccacttt ggtaccggta tccagattct
    38701 gtccggctcc cgcaaaggtg atgccggtga attccgctct ggccccctta ccgttcagca
    38761 cgctcatggg gtacagatag gatacgtggg atccgaagga accggatacc cattctatgg
    38821 taccgccttc ttccaccaac gcccgcttgg tattcagatt gtacatattc ttggaccagt
    38881 tctcgatggt ggagtaacgc agttttgcat ttttccccac gaacagctcc acacagccgg
    38941 catggaggtt cgccacgttg tacttcgggg cggaacagcc ttcgataaaa tgcaggtatg
    39001 caccctcgtc cacaatgatc agggtatgct cgaactgtcc ggctcccggt gcgttcagtc
    39061 gaaaataaga ctgcagggga atcgtcacag acactccggg gggaacatat acgaaggagc
    39121 cgccggacca cacagcaccg tgcagcgctg cgaatttgtg atctctgggg gtcaccagct
    39181 tcataaaatg cttcctcacc atatccgcat attccccatt cagggcactc tccatatcgg
    39241 tatagactac gccctgggct gccacttctt ccctgacatt gtgatatacc agctcggaat
    39301 cgtactgggc gcccacaccg gcgagggact tccgctccgc ctgtgggatc cccaattttt
    39361 cgaaggtatc cttgatgtcc tccggcactt cggaccattt ggcgctcata tgggtattag
    39421 gttttacata ggttacgatg tcttccatgt tcagaccctc aatagaggga ccccagtcgg
    39481 gaacttccat ctcattatag atctgcagag actgtaatct gaaatttgcc atccatgcgg
    39541 gatccttctt tttctcggaa atctccgcca caatatcggg agtcagtccc tttttaatac
    39601 gatagacgtc cttctcctca ttacggatat cataaatgct tctgttgacg tcttccacgt
    39661 atgtcttttc acgttcactc attcagatcg tcaacctcct tatgcttcca gaaaacgctc
    39721 gaaaccatgc tcgttgatct cgtctacgag agaagcatcg ccggatgcca cgatcttacc
    39781 attcaccagt acatgggtct tgtccacatg gagagactct aaaattctgg tgctgtgagt
    39841 aatgatcaac agcgcaccct ttttgtcctt ctggtactct tccacaccct tggataccgt
    39901 acgtaccgca tccacatcca gtccggaatc ggtctcatcc aagattgcca gtgagggttt
    39961 taacataagt aactgtaaga tctccgcctt tttcttctca ccgccggaga atcccacgtt
    40021 cagatcacgg tcaccatagg aaggatccat ctgcaatact tccatggcct gctccagttc
    40081 ctttttgaag gtccacagct tgatacggga accgcttctc tgttccacgg cattacggat
    40141 gaaattccga agagatactc cgggtacctc caggggattc tggaaagaca ggaaaatacc
    40201 atccttcgct ctctggtctg cagtttcctc taataattcc ttccctttga acaggatctc
    40261 accaccggtc acctcatagc ggggacttcc catgatggtg tagcccaatg tggacttgcc
    40321 ggcaccgttg ggtcccatga ggacgtgggt ctctccggga cgaacctcta aatctattcc
    40381 atgaaggatg ggcttgtcct ctacacttac ggacaagtta cgcacctgta ataattgttc
    40441 tgacatatac ttcctcctat ctctatcgtt caactaggaa ttactatatc attatataca
    40501 cgcatctgca aatatgcaac atataattcc tacaaaagta ccgtacattc tcggcaaatt
    40561 gtattacact ttgtggttag tccaaactta ctatagagca aaatgcaggc cactgcaagg
    40621 ggaactcatc cttcccctta caatggccta tgtttttttc tttatacttc ttctttgtcc
    40681 agaaggttct tcatgctctt ggtgctgatc cacagggtag atgtattatg cagaagagcg
    40741 gacgtagtgg gaggaatgat tcctgccaca cctaaaatga tcagtccggt gttgatccct
    40801 acgatcatgc ggtaattttt atggatccgc tccatcagag agttgctcaa ttccttcagg
    40861 gtagccactt catacagatt gtctgcgccg atggtcacat ccgcgatctc tctggcgatc
    40921 tgtgcaccct cgctgatggc aataccaaca tctgccgcag acaatgctgg ggaatcgttg
    40981 atgccgtcac cgatcataat gactttatga cctgcctgct tctcatgctc cacgaacctt
    41041 gctttgtcct ccggcaacac ctcggaataa tactcatcca cgccgacggt acccgcaatt
    41101 gccccagcga tacggtcgct gtctcctgtc atcatgacta ccttgtggat tccgagactc
    41161 ttcagctgtc ggataacctc cggagcctct tctctgagag gatcctcgat gcagatcact
    41221 gcagccagct tgccgtcaat tgccatatac aggcgggagt attcctcagg cagctggtcg
    41281 aacagctcct gtctgccctc cggaatctgt gcctgctcat cctcaaatac gaaatggtag
    41341 ctgccgatga tgatcttctt gccttccaga gtggaagaga ttccatgagc tacaatatat
    41401 tccactttgg tatgcagttc ttcatgtacc agctccttgc gggccgcttc acgcaccact
    41461 gcctttgcca tggaatgcgg gaaatgctcc tccaggcagg cagcgatccg aagcagttcc
    41521 tctgcgggtt gtccgttaaa ggataccaca tccgccacgg taggctgtgc cttggtcagg
    41581 gttccggtct tgtcaaatac aatggtggtg gcatctgcca tagcctctaa gtatttgccg
    41641 cccttcaccg tgatctcata ggtgcttgcc tcacggatcg cagacaatac tgctaacggc
    41701 atagacaact tcaatgcgca ggagaaatcc accatcagca cggataatgc cttggtcaca
    41761 ttccgtgtga acagccatac cagaccggtg cctatgaacg tgtagggtac cagcttatct
    41821 gccaggtgct ctgccttacc ctccagagag gatttcagct tctcggattc ctcgatcatg
    41881 gtcatgatct tctcgtattt gctggagccg ccgttttctt tcacacagat agtcagctcg
    41941 ccttcttcta ccacagttcc ggcatagacg gttccaccgg ggatttttct cacgggaaca
    42001 gactcgccgg tgagagatgc ctggttcacc ataccttctc cgtccgtcac tgtgccgtca
    42061 aaaggaatca cattgcccat gtggatcctt accagatctc ctgacttaat ggatgtggac
    42121 ggcaccagga tctcctgtcc gttgctcaca acccatacct tgtcaatgtt taaggacatg
    42181 ctcctcgcca gatcaccgac agactttttg tgcgtccact cttccaggat ctcgccaatg
    42241 ccgagcagga acattacgga acctgctgtg ttgtaatcac cacgcagcac agatacaccg
    42301 atagcagtgg catccagtac cggtacttcg atcttgccct tcgccagagt cttcacgccg
    42361 tgccagagat atttgaaaga cttcactgtg gtgatcacgg aacggatgtc catgggcaga
    42421 aacagcctga tcccataatg cattaccact ttattgatca gctgatcctt atattcccgg
    42481 ttcatctctc tgccggaatt agcaagatag ttctccggca cttctgtacc ctgatatgta
    42541 aaattctcca gtgtcttcag tgcctcctcc ctggaaccgg tatagactac cgtgacatcc
    42601 agggtgcgct cctgaatttt ggcactgaca atattactct gtccatccag atagtattgc
    42661 agtgtatctg cctgtgcaaa gctcatacgg ctctgcagaa tgtgaaccct gagtctcccc
    42721 tttatctcat gtttgatcac aaatctcata atttatgcct gctttcatga aaacacctgc
    42781 gatgccgaag catgcaggtg tcatttattc tgtgttgtaa gattcgatgg attattcagc
    42841 gtctgctgca gtctctgctg cttctgcctc aacctcgccg tcaaactcct gagctgcctt
    42901 tgctgctctg tcctcgttga tctgctgagc ttctgcgtag atatcctctg cgttctcctg
    42961 gatggtggtc gcagtcttca tgacacactc ttttgcgcgc agaactgctg cggtgcagtt
    43021 ggtgtacagc ttcttcgcat ccttgctgga cagtactttg atgcctgcgg ttccgaacag
    43081 aacgcctgct gcgaaaatac cggtttttgc tttatcaatc ttttttaaac atttcaacat
    43141 aattcatttc ctcctgtgcg aattctttgg tttattctat ttatgaatga atattataaa
    43201 cacctatttt tcctatttcc agcttttttc gtttaatgaa aaattttatc atttatcaaa
    43261 aacacaaaag gccagtggat caaaatccac tgaccttctt gtattcctgt aatttttctt
    43321 ttgctgccgg ggtcagattc ttaggcacct gaatctggat cacgacatac tgatcgccgt
    43381 gggcattttt atccttcatg gataccacgc ccttgccttt cagacggatc ttacttccgg
    43441 actgcgtacc ttccttcact ttgcacatga catcaccgta caacgtgggt acacggatct
    43501 cgccgcccag ggctgcggta gtatagggga tattgaccgt ggtatacaga tcctgtcctc
    43561 tacgctccca gcccggcttc tcctgtaccg tcaccttcag atacaggtca ccgctctcac
    43621 cgccggagta accttcacca cccttacctt tcaggcggat acttttaccg gtgtcgatac
    43681 ctgccggaat atgcacctgc acggattcgg acttgccggt agccggatct cttaaggtaa
    43741 aggtcttatc gcagccatgc attgcttcgt caaaactgat ggtgatgccg gattccacat
    43801 cc
//
LOCUS       DAWDHD010000005        39231 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_88_length_39231_cov_13.198208, whole genome shotgun sequence.
ACCESSION   DAWDHD010000005 DAWDHD010000000
VERSION     DAWDHD010000005.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 39231)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 39231)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..39231
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88_length_39231_cov_13.198208"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    16720..16819
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    33516..33615
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT        10133 a         9772 c         7359 g        11767 t
ORIGIN      
        1 tctacagaac ccagtaagcc tttgtgcgga aataccgaaa aattcacccc cttaaatttt
       61 tctgttccaa atttctgcat caatagtttt cctttccata attgttttat cgtaaagctt
      121 ttccaatctc cgttcttgta ttgtatcatt attatcttta tttagctagc tattttagct
      181 taattttagt taaaattatt ttttgttttc tcacttttcc tatgttaaca taaaaacaaa
      241 gttatacgct caaatgaggg agaatattgc catgcatttt gtccaggcga aggggatttt
      301 atctcagaat aatggaatga atatttatcg cggatgctcc cacggctgta tttattgtga
      361 cagccgcagc agatgttacg gttttacaca ctcctttgaa gatatcgagg tcaaagaaaa
      421 tgctcctgaa ctgttggaac ggtcacttcg ttccaggcgc cataagtgta tgatcggtac
      481 aggcgccatg tgcgatccgt atcttcccgc tgaaaaagag cttcaattgt ccagaaaatg
      541 tctggaactg atcgatcgat atgaatatgg tgtcacggta ctcacaaaat ccaatctggt
      601 gcttcgggat ctcgatctgt tgcaatctat caataagaag accaaggcag tcgtacagat
      661 gacgctcact acttatgatg agtatctgtg ccggaagctt gaaccgaatg tgtctactac
      721 gagagagcgt tttgaagtgt tgatgagatg taaagaattg ggaataccga ctcttgtgtg
      781 gatgacaccg attcttcctt ttattaacga taccagggag aatatagaag gcttgcttga
      841 ttattgcctg caggcaggag ttcagggaat tctggtgttc gatgttggtg tcacgctgcg
      901 ggaaggcgac cgggaatatt tctatcaggc gctggatcgt gatttcccag gcattcggca
      961 aaaatatgtc agtacttatg ggaatgctta tgatattccc agtccttatg caaaagagct
     1021 tatgacactg attcaaaaca cctgcacagc gaatggtata ctgtgttccc cgaaagaatg
     1081 ctttacttac ctgcatgaat tccccgagag atacgtgcag cagacgttgt tttaggttcc
     1141 aatggcgata ctttctacaa aaaagtttaa tatagccaac acagtaaaat cgggagcttt
     1201 tgctcccgac atcttgataa cgcaaaaaac aaatatttac attcagaata gaactacact
     1261 cagaaaattg gtagctatag cttcaacacc caaaacaaaa acggcattgt gatcacggaa
     1321 agcaatgtgg tcagggccac gcccctggat gtcagccgat aatcgccatt gtactgctgt
     1381 gccaacatta ctgtcatgct gcctaccggt gttgccatca ttaccagaca aacgcctcgg
     1441 aatataggat ctttgataaa aaagcatagc ccccacagaa gcaatgccgg taataatacc
     1501 agttttatta tagaaaaaat gagtaggcgc acatctgtaa ataaatctct taaccgggtc
     1561 tgcgccaaag agtcaccaat gaccaacatg cttaacggac ctgtgatggc accagtgaaa
     1621 gttgctgtat cttcaaggat caatgggacc ggaagattgg tgatatacag aactatcgca
     1681 ataatgcaag agcctacgcc tatattcacg agctgtttcc aatttatatt tttctgacaa
     1741 ccaccaatct gcattttgtg gatgccataa gtatagatca ggacattata taacaagttg
     1801 aagattacag cgtgaattat tgcattgctg ccatacatga catccagcaa aggataaccc
     1861 ataaaaccaa tattggaaaa aagcgtcatg acctgataag caccgtagtc ctctctgtct
     1921 gcatgaagca atctgggcaa tagttccgcg acagcaagca taattgcaaa atagatcaga
     1981 gcaaccccta gagtaaacaa caattttcct ttattctcca gcctctcagg attcatacca
     2041 gaggcaatga tcagcgcagg atttccaata ttgattacta tacttgaaat tgttttgcct
     2101 gtagtatctt taaacacacc gcttttccga cagatatatc cgatgagcat aatcaagaaa
     2161 aacatcatca ttttctggag taaaagcata caacaccttc ccatagataa atatataagc
     2221 tcgcgaatta tacgttctta caaatgcatc tgtatctact gccaacatta ctttgcagag
     2281 tcaccatcac agcccattat aacacaggaa ggagagggaa ttgcaatttc ctgtccgctc
     2341 cactttaaaa ttcctttgtt ttcatcaaag aaaaaggtct gtaccacatc tgtatgctga
     2401 atacctacta tcagcataga gccatctggt acaaaacaaa actcccggca aaaatcacct
     2461 gccaacattg tatgtccttt cagcatcaac aaaccggttt ccttatctac ctcatagaca
     2521 acaatggaat tgtgccctct gttcccagcc aaaagatatc taccggatgg atgcatctgt
     2581 agtgccgaag tatagttctc ccggtcataa ccatcctcac gtctcgtcga tattacctgg
     2641 acattccgta acatatcttt gcaatccggc tgatactcaa acacaaaaat ttgacagctc
     2701 aattcagata ccatatatag atattttcca tccggactga aacggagttg tctgggtcca
     2761 ctgtcttccg gtgctttcca ctcgaaaact ttttgatacg tttttgcatc ataaagatat
     2821 atataatcaa gaccacaatc cgcaaccgca atatattttc catccggtgt aggcatggat
     2881 gcatgaatat gagacatcct tccatttggt tcctggtgat gctctactcg tctttcctcc
     2941 ccaatgcttc catcctgatt aatgccgtac accaccagca atccattacg aaaaacagat
     3001 actaaaagac tatctttctc ctcatccgct ataacatgac atggccagcc accataagca
     3061 tatctgctat ttaaaaatga aagtttatta tcctctatgc gataagcagc aacacctcca
     3121 cccggtacac ctttatattc cagtgattcc ataaccgcat acagacattt tctcttttcc
     3181 gaaacatata aatatgcaga atcgtcagcc tctactgttg aaacttccct catttcccca
     3241 ttttgagtat ttatctccaa tgtgtaaata cctttcgatc ctctcaagga gttcgttcct
     3301 acgtaacact tcatccattt ctccatatct ttacctttca gtttcgtcat tgtactctcc
     3361 gttgtaaatt tgataagctc ttggcattct tttttcccat aaacgttttc cctcacatag
     3421 atcaaaaagc tccagtcttc cggttgattc cgcaaattct tcatcagcaa tcagcaaaca
     3481 atccagctgt ttcagataca gaaagctcct gggccatccc agtgtttgca aaattctccc
     3541 ttcccggatc attccgtttt gctcaacgac caattcccgg atcagattag ctccccgtac
     3601 tcctacgaac aatcttcctt ttgtccaatc tggacacaaa attgaagaaa gactttttcg
     3661 tgctccggca gggagcaaac tgacacgctg cttttctttc aaacctccgt ctgaattttt
     3721 ctccaacacg atcaattcat tcgataattc tgtaacgagg tatacgattt ttcctaatgg
     3781 atgtatctct agaaatcttg gacctgatcc ccggggctgt tgctctattc tccagttgtc
     3841 ccggtttccc caaagttggc tctcactact atgatatact gcatccgcgc ccagatcggc
     3901 aatccagcac tcctcctgca tccactttac aaaatgcgga tgagacgctt cctgcctgct
     3961 aactaccgga ccatttcctt tgaaaatcac ccttccagat tctttgccga tgcgcccctc
     4021 cgctacgtcc actgatacaa ccatttgttc tccatacaca gcaatcaaaa gagcctttcc
     4081 ctgctcccaa aagctcatat ggctgatccc tctccaaggg aaacagcttt tctccaaaaa
     4141 aaacagttct cctgtaagtt gcttcaaata aaacgactga atataacttc cccctgattc
     4201 ttcctgtcct accgcataca gtattccgtt gggcgcaata cacgcccatt ccagattttt
     4261 cagtggtgca gccaaagttt ccctgctgtc atttctcccc aggtcaatac gatatatccc
     4321 atctgtcgat gtgtttccaa atgtattgca aaaatagaat gtcatttaag tcagctctct
     4381 ccattatgaa aacaaaaact ctacttcctc ttgcgttaat ctcttttctg atgcacgaag
     4441 gctctcttcc agttccttaa ccctgcaagg tcctacgatc ggaataacct gaaaaggctg
     4501 cgcataaaga tatgctaatg ttatttccaa tggagtggca ttcgtgagct tgcatagttc
     4561 gtctactctc tccgctcttt ttaaggattc cgcattataa aacatatctc tggggaaagc
     4621 aaatttatcg tccttgagaa atccctcttc cttatgtaat acaaaaaatc ctgctgcctg
     4681 agaagtatat gcagttaccg gtttaccggt tttttggtga tattcatata tgttctctgt
     4741 catatgggtc tgtacagctg cctccactgc ctcgtcattc ggttttgcca aattaaacat
     4801 aatttcatgc ccaacaaagg atttcaaatt atgctctttc gcgtaagcat ctgcagccca
     4861 cattctttct gccgaccagt tcgaacaagc aaaatatctc acctttcctt ctctgacaaa
     4921 gctgttcaaa gtttcaataa tttcaccaac ctcacgcttc ggatcatctg catgtaaata
     4981 gtataaatct atataatcag tctgtaagct tttcaaactg ctctccagat cctctcgtat
     5041 ctcttccggc gccagccttt ttatgctcgg tgtttcaagt gcatggcagg ctcctttggt
     5101 cgcaactacc agcttgcttc tcaatctatg ctgtttcatc catcttccaa tcaataattc
     5161 agatagtgga agatttcctg gattccacct tccatacaca ttcgctgtat caataaatgt
     5221 acccccattt cctacaaaac aatcgaggat atcgaatgat gcctggtcgg aaatacggct
     5281 cccgagcact ccacctccca acgctatatc tgacactcgc aaatttgttc ctttataatt
     5341 tacaatattc atccttcgct ctccttttaa aacacttctc agcctttcac tgctccaact
     5401 gtcatacctc ttgcaaaata ggactggaaa aacggaaata taatcataag aggtaaaata
     5461 ccgattactg caattgccat acgaaccgtg atggtaggca ttttacccaa ggcattggca
     5521 gcctctgcgc ttcctgccat actttgtgtc ataaactgaa tatccagcaa aattctattc
     5581 aaataatttt gcagactgaa taattttgta ttcgtaagat aaaggagacc attataccag
     5641 tcattccaat aaccaatacc aatcaacaat cctaccgtag ccaggatggg tttcgaaatc
     5701 ggcataataa tactaaagaa aattcggaac tccttagcgc catctaccat agctgattcg
     5761 atcaattcct taggcgtatt gttattaaag tatgttctca ccagcagaac attgaaacca
     5821 tttaccaaaa tattatttgg caaaagtaat gcaaatatag tatttttgac atgtaacgta
     5881 tttacatata ataaataggt gggaaccata cctccgttaa aaagcaatgt aaaaaacacg
     5941 agaaaattga ttatctttct tccgggtaat tcattacgcg aaagtgtata tcccagcatg
     6001 gctgtaagca tcaatccgga aatcgtacct ataaatgtag ttagtattga aatcccgtat
     6061 gcacggaata ttgtctgccc ctgcgttgcc atatagcgat atgcttccag actccactct
     6121 ttgggccaaa aggaataacc atttcggaac acactatcat tatccgacag agaagacgtt
     6181 accagcaaca acgtaggaag agtacagaaa agcacgaaac aaattaaaac tatatgtgct
     6241 actaatgctg atttgtttac tttcattcgt ttcatctcac acctcctaaa acagagaatc
     6301 ttccgggctg atcttccgaa gtaacaaatt tgtaattaaa acaattaaaa agccgactac
     6361 tgactgatat actcctgctg ctgcagacat acccacgtct cctgtctgta tcaatgtgcg
     6421 gtacacatag gtatctattg tatttgtagt actgtatatt gctcctgagt tctgcgtaac
     6481 ctgataaaat aaaccaaaat cagaataaaa aatctttcct accgccaata atatcatcat
     6541 tataataacc ggtttcaaca ataccagtgt gatataccgt acctgttgaa gctttgatgc
     6601 cccatccagt tgagccgctt catagtaaga cttatcaata gaaataacat tggcataata
     6661 aatagttgcc tgaaatccaa atgacttcca tgtatttacg atcggtaata ttataggcca
     6721 gtactttgac tcattgtacc aggatatcgt gctttttccc aagggctcca ggatcccttt
     6781 attaataaag cctacatccg tacttaagaa agcaaatacc agataactga taatgacgat
     6841 agaaaacaaa tatggcaaaa gaatcactgt ctgatatact tttaaagcct ttttgcttgt
     6901 cacttcgctc aaaagaattg ctactaaaat acctaaaaat gtattaatta taataaatac
     6961 cacattgtac aatattgtgt ttctggtaat aataaaagca actgagtttt taaataaaaa
     7021 caaaaaattt ctaaagcctg cccaatcact tccataaatt cctttgctgt agtcgaaatc
     7081 tttaaaagct accaccatac ccgttagtgg taaataattg ttaataaaca aataaaccaa
     7141 tgccggcaaa gccataatgt atagtggcaa aagctttttt atttttttta atcttttata
     7201 cataccaggc tcctttgaat agaaaggtca gttgtgttat acctctgacc tttccgagaa
     7261 actttactta ttttgttgcc gcccactcgt ctaactgccg ctgcttttct gcaataacag
     7321 tatcaatccc tgcatccttc agtgcctgtc ggaatttcgg taaattttca tcaggatcaa
     7381 ttactccatt ctccagaggc agacgatact catccattac tgcctgtaca gccgccgctt
     7441 ctgccttcac aggagtggtg tcaaacacaa atcccagcgc tttagatttg attgcattat
     7501 cattaaattc ctcatatttt tcatttaaat cttcgggatc cgtattccaa atataactca
     7561 gataacgatt tccaaaagcg taagttgcac tcagagtata gccggatgtt tctccagtta
     7621 caccgtccgg ataagtaatc cacttttcgc ttccttttac atgctgatag tgtgtacctt
     7681 caatacccca taccaacgca ttatatactt ctgcatcact ataaagcaga ttgacaaact
     7741 ccaacacttt ttcaggattc ttacagctga cagggatcga ctgaataaca gctccaaccg
     7801 tttgtgtatt tccaaaagcc ggatttacaa ttgctgtact gcatggaata cctgtcatat
     7861 tggtgctgtc tgtaggcaca cccggatgaa tcagggacat tgtggatgcc acttttcctg
     7921 ttttgaaata agctactcct gtatcaggat tcgttgaagc atctggaaga atatagccct
     7981 tctcattcca ctctctggct ttcctcacac actcctcgta atattctgta tcaaagagat
     8041 tttgcacggt cagatcgccc tgtccccaat ccatcaggac tcccatggta tcacccagcc
     8101 catcatatgt tttaaaaagg ctttccagaa tgctggttcc ctggctttgc ggctgcgtta
     8161 catacttgtc cggttctccc tctttaattc tggcaaaaag gtcatctaat tgttctactg
     8221 ttaatcctgc atcagcatca attccatatt tctcgataat atcattgcga agaatcagtc
     8281 cgtatgcact tgccatatca cgtatggtcg gaaatccata tatttttccg tcaactgtgg
     8341 tagcgtccat atagtcgccg attacttcca ttgcatccgg tgcatattta cgaaccgttt
     8401 cttcatccag cccaagcagt ttattggaag tgacatcact actgaatgta gttctggctg
     8461 atgcatttac cagatccagc ttttcgccac tggtaatcat cagattaacc tcctgctgat
     8521 agtttgctcc tgtcataggc aaaagcttaa ccttggcacc aatcttgtca taaacaatgt
     8581 tgcttagatt ctcctctacc aaatcccaat ctgcctgctg tacccataca tcaaatgcat
     8641 aaacaatggt atatggttcc tctccttcct gcacagtgtc attcgtttca cttttgtctg
     8701 tctgacctac gctctgatta ccgctttcat ttccacatgc ggaaagcact gtacatgctg
     8761 ccaacaataa ggcaaatact ttacttacaa ttttcttttt catatcattt tctccttcct
     8821 tttatgtatg ttttgtatct tttgtaatta aactatatct cttataatta tttctttctc
     8881 cttgaatttc gaccttcact tatgaatttt atacgctttt tgttttatcg aaatccagct
     8941 catgaaatgg atctttgtcc tctccatcat aatccgtcag cttactcgcg atccttttca
     9001 gataactatt tgtgtttgca agagatccgc taataatatc ctcttctgtt attttggcaa
     9061 ttaaaatttc tctggcggaa gccatcacct ctttcatatt ggatagataa cttccacgtt
     9121 cccgatcata tgttacatag ataaatccgt catctgcttc ctttgcatcc ggataagaaa
     9181 cttctctctc gtctaatagt aatttataag ggaacgtttt tccttcatcc tctgacagca
     9241 ttgccgtaag attatttctt ccactaaact taaaatgatt gatcagcagg attctacctg
     9301 aacgcaatct tgtaatatgg aatctcgaac agggaccacc atagccagtg tcaaaatctt
     9361 tactccagct tcttcctcca tcataagaat tcgatgcgcc aatcccatac tttgtcctaa
     9421 caaacattct taggcttcca tccttcatct ccaagatcat atgttcatca taatgccggt
     9481 ctttcacgtc tgcaaagcca agcttctgaa aggttttccc gcaatcattg gtagcataaa
     9541 caaaggagcc tttttccgta attttagtat catattcttc tgataatact cttataccat
     9601 cattccatac agcaatcggg aacaaccatt caccagattt caccactata ggtttattca
     9661 tcattacatt atggcctacc agaaattctt ctccccaaac cagctcctct gcatcaggat
     9721 ctcggcaaac agatgcatat aaaccatcgt ccggacactt tgcccatgtc agccaaagtt
     9781 ctcccaatga atctatccaa aggcatgggt caaagcatct tccattatct tcttttacaa
     9841 taacaatcgg ttccgagaaa tgattccctc cgtcatccga cttaattacg atcacataat
     9901 ttcctatttc ttccttgact ccacctgaat agaaagtaag gaaaatcctt cctcgttttg
     9961 tcacctcaat gcttgggatt ccttgccata ggcgttttga agccctgtat ttttgtaatt
    10021 cctttgtttc cgtatacaac ataatcgttt cctccaacag acttatatcc atgttttctg
    10081 catcttcttt aaaaatacag gagctgttat atcaacggaa caacggacaa tgcagcccct
    10141 cttttttcat tttctaaata tacagtatat ataatatata atttatgatc tgcttcatag
    10201 gcgcatgggt aacaccacgc ttttccataa cctaatctgt cggaaaagcc attttgtagt
    10261 ataatgcctg tctcaaaggt actttctttg ggtttggaga atgcgagtac aagtttttct
    10321 cttcccggat accaatttcc tattatataa tttctaccat cagaaagcgt gcccgcatat
    10381 atttttgaat ctgaaaaagg aatatcatag gaaaaggcat ccgaccatgt ctcgccataa
    10441 tcctgtgata aatatacaag cggaactttt cgctcgtcat ctctcgaaaa catggcaatt
    10501 ttattatctt ctatgattgc ggtaatttcc ggatgtacca gcttagaacc atcaggtaag
    10561 tctccatttt cctgaatata tactaatctc caatcatcat ctattttccc ggaatccgaa
    10621 ataagtaccg ccggagtatt cgggaagcca tctagttcag caatacgtcc cggcaaaagc
    10681 aatttcccat ttgccatctt atacacattc gtatttagct taaaaggaat cggtttggac
    10741 cataaaaatt caaaacagtc cttttcctct tgaaatacat aaagatcaag agcatgaata
    10801 tgatctgctg ataccatttc attaataagc atatatagtt tatcttcgca aataccgtac
    10861 actggtgggc agaacaaaat tttgccggtt tgatcctccg ctatcgtttc aatatcactc
    10921 catgtctttc caccgtcttt tgaccttttt tgtctgattg gagtaggtcc gatcagttct
    10981 ttttcctcgc aattatacca ggcagcaaat agcgttccat gatattctat aatagctgct
    11041 tcatgcaaaa aagcatattt atcatccggg ccggcgactt catatatctc tgtttcactg
    11101 ttatccttta agcttatttc tttttcagcc atattcagat gtcctaacat attatatagt
    11161 tgtttatctt cctttgaaaa aaaatcaaac attttgttcc tccctttcca tatcattctt
    11221 ttcatttagg atcaactata gtataatctg ctattatgtt ctgactctac ttgaaactcg
    11281 accttcactt atgattttta tacaaaaaga cctgatcctc aaagatcagg tctttccatc
    11341 ccaccgtttt ttcgatattc atttggtgaa attcctactt ctcttttaaa cattgcagaa
    11401 aaattagaca tattttcata gccgcaccgg gatgcaatct cacatacgga aatctttggg
    11461 tttaacagca atccttttgc catatttacc tgttccctta aaatatagcg atttaccgaa
    11521 catcccattt cctttttaaa tcgtctatcc aggtaatccg gatttaaata cagtgctgaa
    11581 gcaactttta tcctattcag ttcctcatct agatgggtct gtatataata aatcgtttgt
    11641 tccctgatcc cacttaagtt cgatacatcc tgcatgatcc tggaaacagc tcccatagtt
    11701 tcctcaatcc atgcatccat ttcctccagt gaaccaattg ctgttactct gttacatcca
    11761 ttttctctat catataaaaa ttgatgtgcc tgaattccat tttcctgaag tgtcgaatag
    11821 aacaactgtt ccaaatcata ctgaagcatt tgaagtgctc tcctatttaa ctttgcctca
    11881 ttgtcttttc cccaaagcca ttttcggatt atatcttgaa gttccacagt cttgccatta
    11941 tcaatatatg ctttccatag cgtcatatca ggcataccaa ttgcttcact gtttttaggc
    12001 atatttataa tatccaaaat ctgtcttcta ctgcaaacat tattccatgc aaactgtcga
    12061 agcttatcta ccatggactg tacttctctc cctgtacaca cttctcctat gcatattcca
    12121 attttttcgt ataatctttc tgtcttaaat tgctgtaata tcgcatcttt taatattatg
    12181 ccatttcttt cagtgccaga actcagccac tcgtctgaaa taaaaaccag gcctgcaaat
    12241 tctttttgag acaataccgg aagcagaaag tcaccagcca ctgttcccaa gtcctctttt
    12301 accttcttcc tggtctgctt tatgtcttct tctgttttgt catttgcata ttgataaaat
    12361 aaaattggga tcagccttct atcttgctcc tctttccatt gcttctcctc atattcttca
    12421 cactccctgt tcggatcatc acgtagtcct ctgagcattt cctcccaata tttttctttc
    12481 catactggtt ttaactggtt ccatacaatt ccttcttcct gtaactgcgc ctgatgacgc
    12541 tctctttcaa tcttagtgac aagatttttc atcaccttaa taatatcatc ttccatgacc
    12601 ggtttaagca gataatcata acatccgtat cgcaaggcat ttctgacata attaaactcc
    12661 gcatggcttg tcaacatgac gatttctaaa tccggaaaat tttcctttgc cttttttgat
    12721 aattccaatc catctgtctg aggcatttca atatcgcaga tcataatatc aatttttttc
    12781 tcatttaaaa ttcgaacagc gctaactgca tttgtagcag aataaatctc cgtaataccc
    12841 agcagctccc agtccgttac tgtaactact tcttctacca ccagatattc atcatctaca
    12901 ataagtaagt tcatagctga tctccattcc aacatctttt tctagttatc ttatagtttc
    12961 ccttttgttc attttggtac atttatgaca acgctaactc ctccactgtc ccgatttgta
    13021 accttgacac tggcagcctc tccataatgc atgtgcattc tatctactat attttttatt
    13081 ccaatatgct ccctatcgtt tttcacaatt ttctcgtttt ggtttatttt tttaagaatg
    13141 ttctcctcaa ttccttctcc attatcctga atttcaattt ttatattgtc tccctctact
    13201 ttggctgaaa cgacaatcat caaatcattc gagaaatccg tggaatattt gattgcattt
    13261 tccacaaaag ttaatacaca catagccggc accatgctga tttctgcttc cggctctatt
    13321 tcacactgaa gatgcatagc acccgcaaaa cgaaactttt gaatcgccat atagtttaac
    13381 ataaaattga tttcctcctt cagaggaacc atttctcctt gacgagcaaa cacactttta
    13441 aaataattta tcagatatgt tagaagaata tttacctgat tgttatcttt tcttttattc
    13501 aacatataaa tagtatttaa agcatttaaa aagaaatgag ggttaatctg aagcattaaa
    13561 tattgtttat acagctcaga ctgctttttt tctttctcat aaacatcaat tttcagaatt
    13621 ttaatttttt ccatcatatt gttaaagttt ttactgacaa tatgatactc ttcccagttg
    13681 tcaaactccg gcattttaag atctattcct tcttcatcca ccttttgcat tactgataca
    13741 atccttttta ccggatttgt aaaccagtga tacatggaaa tataaagagc aaaaagcagt
    13801 atgatcaaaa tcacagcaaa aaccatactg aagtttaata acgttctgat ttcttcaaat
    13861 agcttttctg aaggaattac aattcttaca cccacatctg tttcttgaat tggcagttct
    13921 atttttagat ctttccctga ctcgttgtct aaaatcattg tgcctatttt atagtaagaa
    13981 acataataat cacttagttg tacagcttgc attgtttcaa tcagtcgatt aaggctcact
    14041 gcacagccaa tccccacacc atcctttgat acaactctga ttaaatattg ataaccttct
    14101 gcatcaaaca gctcccattt ttctgtaggt tctctactct ccaataacct tttcaactgt
    14161 tctctgatat tctgactttg tatttttgct ttcttttttg cagattccac catcatgata
    14221 tctgcatatt tgtcttgaaa tataaaaaag taatccgcag aactataaac aagaatatcc
    14281 tcattcaatt gattatacag ccgctgtctt gacaaattgt tcatagattc tttatcctct
    14341 tcgcaattag tcataccata ataatccgct gtttcgctta ttcgaagaag atagtccgaa
    14401 atattgctca aattattttc tacatcgcgc gtatagtact ccaccataga atgataagtc
    14461 ttttttgcct gtttccaagt cgtacgagct gcactgacgc aactacataa aagcattatt
    14521 atcatgcaaa aaataattac agatataaga ctattcatgc aaattacaac agattttttc
    14581 attttgctta acatgatttc gcctttctta agagcaccaa ccgttgtgac accagcaccg
    14641 tatatatatt tctatctaat atagtatcat attcttgtgt aacttccgct tgaatatcga
    14701 cttttgatac ctaattttcg acttctttta cctgattatc gacttcttta caatattgtc
    14761 ttctttttca tttattattt cgagttcatg ataccatgac ctaaaagaag ccgccagtct
    14821 ctgttttcac ataactggcg gcttcctctt gatatcttat attctcttac agaaattctt
    14881 tgacagactt gtaaacgccc ttggcatcca gaccgtactt ctctaccaga gcagcagcgg
    14941 aaccggactc accgaagaca tcctgcatgc cgaccttctt cacaggcacg ggatgatttg
    15001 cgcacagaca gtcgcagaca gcgcttccca gtccaccaat cacggaatgc tcttccacgg
    15061 tcactacctt gccggtcttc ttggcgctgt taatgatgat ctcctcgtcc agaggcttga
    15121 tggtacagat atttacaacc tctgcactga tgccttcctc ttccagcatc tttgctgcac
    15181 ccagagcgga atctacgcag ataccagtgg ctacgatggt gacatcctta ccttcacgga
    15241 ctacggttcc cttaccgatc tcgaaatgat aaccaggatg atcattgatc acgggtactg
    15301 cagcacgtcc gaaacgaata tatacaggtc cttcatattc cagagcggca cgaacagcgg
    15361 ctcttgcttc cacatcatcg gaagggcaca ttactaccat gccgggaatg gtacgcatca
    15421 gagcaatatc ctcgttacac tggtgggtag cgccatcctc acctacggtg atacctgcat
    15481 gggtcgcacc gatcttgaca ttcagatgag ggtaaccgat agagttacgt acctgttcaa
    15541 aagcacgtcc tgctgcgaac attgcaaaag agcttacgaa gggaatctta cctaccagag
    15601 acagtcctgc agccacaccc atcatgttgg actctgcgat accgcagtcg atgtggcggt
    15661 cgggatatgc cttacggaag attccggtct tggtagctgc tgccaggtcg gcatccaata
    15721 cgaccagatt ggggaattct tctgccagct ccttcaggga gttaccatag ctttctctgg
    15781 ttgcgatttt tttcacggta ttctctgcca ttactgctca cctgctttct ctaattcttt
    15841 ttcgatctta tcaaggtctg ccattgccac tgcatactcc tcatcgttgg gagccttgcc
    15901 gtgccagtcc acactgttct ccatgaagga aacgccctta cccttcaggc tatggaatac
    15961 gatacaggtg ggcatgccct tggtctctct tgcttccttg aatgctgcac ggatcgcatc
    16021 aaaatcatga gcatctgcat tgatcacatg gaaattgaat gcctcgaact tcttatcgat
    16081 aggataagga gagcatacct ggtcgatagg tccatcgatc tgcagattat tgttatctac
    16141 cataacacag aggttgtcca gcttacggaa gcctgcgaac atggctgcct cccatacctg
    16201 gccttcctcg atctcaccgt cacccagcag tgtgtataca cggaaatcac gattctccat
    16261 ctttgcaccc agtgccatac ctactgctgc ggaaatgccc tgtcccaggg atccggaaga
    16321 catatccacg ccggggatat ggatgcaggg atgaccctgc agataggagc ccaggtgacg
    16381 cagggtaggc agatcctcta ccgggaaata tcctctgtaa gccagtgcgg aatagagtcc
    16441 gggagctgta tgacccttgg acagtacgaa tctgtcacgt tcctccatat cgggattctt
    16501 cgggtcaatg ttcatctcct caaaatataa aaatgtaaaa atatctgcgg ctgacaggga
    16561 tccaccggga tgtcccgctt tcgcaccatg aactgcggtc acaatgccct tgcggacatc
    16621 attggcacgc ttttgcagtt ctaaattctt cattgtctcc tccattccgc catattggct
    16681 tgtctgttgc gatttacgtt gttatactag catacttccn nnnnnnnnnn nnnnnnnnnn
    16741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16801 nnnnnnnnnn nnnnnnnnnt tccgccatat tggcttgtct gttgcgattt acgttgttat
    16861 actagcatac ttccgaatgc tagtctagta aaagaattgc tcttctaccg taaaatattg
    16921 cagtggcaaa ttccagcata cagttcagac ctccattcat aaatttgttc gttccactca
    16981 caaattcatt tcatggaggt gcgctccgct aatagaattt gtcatatagt gctctgtgtg
    17041 caagcaccct cgcactataa gccaaattct atttctgaac tgtttgacca tagtatgcat
    17101 tctttccatg cttgcgatta tagtgcttat cctgcagctc ctgcggtgcc ggctgaattc
    17161 tggggttgat cgtctctgca cgataagcca tttttgccac ctcttccagt actaccgcat
    17221 tgtggacagc ctctctggca tccttgcccc aggcaaacgg tccatgattc ttgcacagca
    17281 cagcgggaac tgccttggga tccttgccca gacgcttgaa ctcattgacg atcagatgtc
    17341 cggtattttc ctcgtaagca tcctcgatct cttccttcgt caggcagcgc acgcagggaa
    17401 ccggaccgta gatataatct gcatgggtgg ttccgtagca gggaatgtct cttcccgcct
    17461 gtgcccaact ggtagcatag gaagaatggg tatgcacgat accgccgatc tccgggaatg
    17521 ccttgtacag ctccagatga gttgcagtgt cggaagaggg attcagatcc ccttccacct
    17581 tattgccgtt cagatccatg actaccatca tctccggtgt cagcttgtca taatccacac
    17641 cactgggttt gatcacgaac agtccactct ctctgtcaat ggcgctgaca tttccccagg
    17701 tgaaagtcac cagaccatac cggggcagat ccatatttgc ttcatatact ttctgtttta
    17761 attcctctaa catatactta atctcattcc tttccacagc ctgccaactt tccgacacat
    17821 aggtatcgtg tacttccgta cactatgcag gctcaaattg catctgaaaa attattttgc
    17881 cgcaacggct gccttttctg cgggaagcag tttcttgtac tcttccagga aagcatcaaa
    17941 tcccttcaca tcctcgacat ccgggttcag tgtggttccg gtctgtcctg cgaagatctt
    18001 gtcattcaga taggaagaaa gagtctcgcc gtcttttttc tccttcatga aaactgccag
    18061 aatagcaata ccccaggcac cgccttcact ggctgtatcc ataacggtga tcggtgcgtt
    18121 catgcctgct gccatgacac gctgtcccac cacgggagtc ttgaagaagc cgccatggcc
    18181 catcagggaa tccacggcca catgctcttc ttttaacaga atgtccatgc caatcttcag
    18241 ggtagcaact gcgctgtaca gatgggaacg catgaagttt gccagtgaga aatccgcatc
    18301 gggagttctt accaccatgg gacgccctgc atccagtccg gtgatgggct ctcctgagaa
    18361 ataattgtat gctgtcacgc cgccgcagtc cgcagcacct tccagtgcct tgcggtacag
    18421 cacgccgtac agttcattct tatctacttt tacgccaaag ctctctgcac attcgcccag
    18481 aagatttacc caggcattca gatcggaggt acagttgttg cagtgtacca tagctaccgg
    18541 cataccgtcg ggagtggtga ccatatcgat ctcttcgtgt actttttcca tagccttctc
    18601 cagtaccacc atagcgaaaa tggaggttcc ggcagagaca ttaccggtgc ggactgccac
    18661 actgttggtg gctaccatgc cggttcccgc atcaccttcg ggagggcaca tgggtacgcc
    18721 gggctgtaag gttccggtgg aatccagtaa ttttgcacct tccgtggtca gaacaccggc
    18781 aggctctccg gctaccagta ccttcggcag aatgtcacgg atcttccagt caaaattgtg
    18841 taagccatcg aactgatcca gcatcttctg atcaaaatcc ataatggtgg aatcaatggg
    18901 gaacatacct gctgcctcac cgatgcccag caccttttct ccggacagct tccagtgaat
    18961 gtatcctgcc agggtggtga agaatgcgat atccttcaca tgatcttctc cgctcaaaat
    19021 agcctgatac agatgtgcaa tgctccatct ctggggaatg ttgaaattaa ataccgggat
    19081 cagcttcttg caggcttctt ccgtcatggt atttctccag gtacggaaag gcaccagaag
    19141 ttctccggct gcattgaacg gcatatagcc gtgcatcatt ccgctgaatc ccagagagcc
    19201 cagggtagtc agcttctcac cgtattttgc ctctacatcg tctgtcaggc tcttgtagca
    19261 tccctgcagg cctttccaga tatcttccag gctgtaggtc cagatgttgt tctccagacg
    19321 gttctcccag tcgtaggttc ccatggcgat aggtgcgtgg ctctcatcta ccagtaccgc
    19381 tttgattctg gtggaaccaa actcaatacc gagggctgtt tttcctgttc tgatcgcttc
    19441 ttttactgct tcgttcattg tgttctctcc ttttcacttc tttatttatc tcatttttta
    19501 tttccgtttt cagtgcattc cgcaacagat tcccgctcta cgacacgggt gtcaaattcg
    19561 taagtcagat cctttttcct gcccgcgatc atctgtaaca atgtctccgc tgcttttttc
    19621 cccagattct ccttggggtg cggcagggaa gtgatgggca cctcgctgtg tctcgccaga
    19681 tccgaatcgt cgatgctgat gactgacaca tcttccggta cccggatatt gcgttctgtc
    19741 aacatccgaa tcaactgaaa agcaatctga tcattgtatg ccagaagggc ggtgcattcc
    19801 tccacccgat tcaggattct gtcccggcag taaccaagtt gcttcgattc gtccgtgtcg
    19861 atccacacca tccgggaatc cgtcacagtg agcccggcct cttccatggc cttcagatat
    19921 cccagataac gctgtctgcc ctgaccgtca tccagcttca ggattgcccc gatcttctga
    19981 tgccccttgt caatcagata tcttacggcc ttgtaggccg cctccgcatc atttaacgat
    20041 acatgcggtg cctccagttc gggataatag gtattgaaaa acagaagcgg tatctttctg
    20101 gccatcaggt gccggtacag atccaggttc ggattcggca atccgctctt tgtcccctcc
    20161 atgatcactc cggccacatc gtcccgtttc aacagatcct tcagaatact tttctcccgg
    20221 tccagcgtgt tgtttgtgaa atagatctgg acagaaaacc cgctttcaaa taaagtattc
    20281 tgcattccct gtaaaattct ggggaaaatg tagctgtcca cataggtagt caccacggcg
    20341 atgctgttgc gctgttccag attcttccgg cggtcaaagc tcacataagt gccactgcca
    20401 cggatcctct gcaccagccc gtcctcctcc agcgtgccga tagctttccg caccgtctgc
    20461 ctgctgatat ggaactgttc cgacagctga ttctccgagg gaactttctc tcccggacct
    20521 agttgtccgt ctgagatctg ttcctgtatg taattaatga ggttttgata ttttgtctcc
    20581 ataatcactt tcagtataat cagaccaccg tacaaagtca atacatcttt tacctgtttt
    20641 aggtacattt gtatatagtt gtatggtgta caaccttgca gtaagtctat cccgtggagt
    20701 attcacatat actcttacca acactctttt tcatcaggta atcatcgtgc gtaaacgtcc
    20761 ccctctctcc ttcccacaac tactcgtatg tattagtctt ctctgcgccc tcaccggtgc
    20821 tctgaccctt gtagcttccc atacctcccc ggatagacgt tttgagcaat tcacctctca
    20881 actttttcag gaggaaatga ctggcagtac cctgaatatg cactacacca ttgccgatcc
    20941 aaagaccttt ggcatctccg aatatgaacc tgtgcttccg atctaccact ccggccagcc
    21001 ggaagacaga aaagaacact gttccgactt attgcaccgg cttgaccgta tagaccctga
    21061 cagactttcc ccggaaaatg cctatactta ccggttattg catcgctctc tggaaaatga
    21121 tctggctctg gcagattttc cttattacaa tgagcctctg tcaccttcct ccggaatgca
    21181 gtcccagctt cccgttctgc tggcggaata caccttccgc acaaaacggg atgtcacgga
    21241 ttacctggct ctcctggatc agatagatga ctatttttcc tccctgcttc tctacgagca
    21301 ggaaaaagct gccgcaggct tctttatgcc tgcctgctcc tcagaaaaag tccggaagca
    21361 atgtgacacc attgtcacaa ccgaagaact ggcacaggga acccattttt tacagactac
    21421 ttttgaagac cgcctgtcag agctacagaa gcagggactt tttacaccgg aggagactgc
    21481 ttctctgatt gagaccaacg accgcctgct tgccacggtg gtgcagcccg cctatgacgc
    21541 cctgtccgaa gggcttcttt ctctggaaac ttccaccaat gccgattcca ctgcatccga
    21601 aacaactgcc aatgctgctt cgggcgaaaa caacagtgcc cacaatgggc tcccgaaagg
    21661 gctcgccctg ctgccggacg gaaagaccta ctatctccat ctgctgtttt ccgagaccgg
    21721 ttcctcccgt tccgaaaaag aactggtgca gatgctgctt gtgcaatttc agaaagagca
    21781 atccgcaatc cgcaatctgg cttcccagtc cccttccctg attacactgc tttctgaaga
    21841 aaatacggca gttttccctc tggcagaacc ggaagaaatg ctctcggatc tacaggctcg
    21901 tatgaaaaat gacttccctg tcagcagtcc cgttcccacc gtcactgtca aagatgtggt
    21961 ccccagtctg gaaccctata gtgcaccggc cttttaccta accacgcctc tgggagattc
    22021 cgacaataat gtcatatata taaaccgccg gaactcccct cagggactgg agctctatac
    22081 cacactggct catgaaggat tcccgggtca tctctaccag accgtgtaca gcaaccgcat
    22141 cttttccgac atgcacacgg atcctgccag aaaactcatc tggtacggcg gttatctcga
    22201 aggctgggcg ttgtatgtgg aatttctttc ctatgattat gcggccactc tgttggaaca
    22261 ggccggacag tcggatgccg cacagtcggc ccggctggag aagcataccc gcagcttaca
    22321 gttgtgcatg tacactctgt tggatctcct gatccacgga gagggtgccg gatacgatca
    22381 ggtggcagag gttcttggga aattcggtat tgattctccc gggacctgcg aagcaatcta
    22441 cacttatatc gcagaagagc cctgtaatta tccgaaatat tacatcggtt atctggaaat
    22501 tctgcagctg caggacaccg ccagaagtca ctggaaagat gcttactccg accttagatt
    22561 ccacaccttt tatctggaac agggaacttc tgatttttcc tcactggaag acctgctttt
    22621 aatgacaaat taacgttttt gtgataggat aaagtgtacg gatcatcagg ttggaggaac
    22681 ccatgacaca gtttacaagc aaggctatcg aagaatcctt tatccggctg ctgaatgaac
    22741 ggccgctgga taagatcact ataaaagaca tcgtggatga ctgcggtatc agccgtaaca
    22801 ctttttacta tcattttcag gatatcaccg ctctgctgga acacattctg aatgccgatg
    22861 tggagcgggt actgtcacag catctggata tggactcctg ggaggacggc tttatctccg
    22921 ctgcccggtt cgctctggag aataagcgtc tggtctacca tatttacaat tccgtcagca
    22981 gagaacgggt ggagcgctac ctttacagta tcgctggaga ggtcatgcgg ctgtatgtat
    23041 ccaggatcac ggaacaggtg gaacatgcgg ctcataaaaa agtatttccc gaagatcaga
    23101 aaatggtggt agatttttac aaatttgccc tggttggcat gattttagac tggctgaaca
    23161 ccggaatgaa gaaagatccg gagggactga tccggcgtgt cggcgagatt ttccacggaa
    23221 atatcgaagc agccctgacc cgtgtggccc gttaatttgg acatttgtcc atgtttgcac
    23281 atattttgga ttatttaaga gcaatgcgca atttttgtgc attgcctttt tttgtccgtt
    23341 ttcaagactg gattccttgc atacaatacc aattgagaaa gaatcgacaa cgaaaggagc
    23401 cctgttttat gaaatttggt aaatgggtcg taaaatgccg tattcctatt ctgatactgg
    23461 cagttgcact actggtccct tccctgatcg gtatgatcat gacgcggatc aattacgata
    23521 tgctgactta cctgcccggt gatattgaca cggtagtggg tcaggatatt ctgatggatg
    23581 aatttggaaa aggagccttt tccttcgtta ttatcgaggg aatggatccc gaggatgtct
    23641 cctccctccg ggaagatatt tcccatgtag atcatgtgga caccgttctt tggtatgacg
    23701 atttcgcaga tgtttccgtt cccatggaaa tacttccttc caaactatat gacgctttca
    23761 attccggtga cagcaccatg ctagcgatct tcttcgatac ctccacctcc tccgacgata
    23821 ctatggaggc gatcacagcg atccgcagca ttgccggcaa gcagtgtttt gtctccggta
    23881 tgtccgccat ggtcaccgac ctgaaggatc tgtgcgaaaa agaggaaccg atctatgtag
    23941 gtattgccgt tgcactggcc tgcgtagcca tgatgatctt catggacaac tggatcacac
    24001 cgtttgtttt cctgatgagt atcggtatgg caatcctctt aaatatgggt accaactatt
    24061 tcctcggaga gatctcttac ctgaccaaag cactgtccgc tgtcctgcag ctggctgtaa
    24121 ccatggacta ctctattttc ctctggcaca gctacgaaga acagaagagt atgtatgagg
    24181 ataacaagga agccatggct gtagccatca acaacactct gacctccgtg gtcggaagtt
    24241 ccatcaccac ggtagccgga tttatcgctc tgtgctttat gagctatacc ttaggtctgg
    24301 atcttggtat tgtcatggcc aagggtgtca tcctgggtgt catcggttgt gtcacaacgc
    24361 ttccttccat gatcctggta ctggataagt tattacagaa gaccagtcac aaatccctgc
    24421 tgccggatat gagaaaagtt gcttcaggta tcaccaaagt attcccggta ttcctgatcc
    24481 tgttcctggg gctggtactt ccttcttatc tgtcttataa ggcaaccaac aatgaagtgt
    24541 attacgatct cggtgaaacc cttcccgaag atatggccta cgtggtagcc aacagcaaat
    24601 tgcaggaaga cttcggtgta ggtgccaccc atatggtact ggtaagcacc gatgtatctg
    24661 acactgacgt acgtgccatg atccacgaga tggaaaacgt cgagggtatc aaatatgctc
    24721 tgggtctgga atccgtagtc ggccctctgg taccagagga aatgcttccg gaatccgtaa
    24781 aagaagtatt aaagagtgat gactgggaac tccttctggt gaactccgaa tataagaccg
    24841 ctaccgatga ggtaaatgcc cagatcaatg agctgaatac tattttgaaa aaatacgatt
    24901 ccaagggcat gctgatcggt gaagctccct gtaccaagga tctgatcgag accaccgacg
    24961 aggacttcaa agttgtaaat accgtctcta tcgtagcgat cttcgtgatc attgctctgg
    25021 tagaaaaaag tattaccctg ccgctgatcc tggtagctgt tatcgagttg tccatcttca
    25081 tcaatctggg ccttgctcac ctgacaggaa cgtcacttcc tttcattgcc ccgatctgta
    25141 tcagtacgat tcagctgggt gctactgtgg actacgctat tctgatgacc acaagataca
    25201 agcaggaacg ctacgaaggc agggacaaac gtgaagctgt caccaacgcc ctgaaggtat
    25261 ccattccttc cattatcgtc agcgccatgg gactgttctc cgccaccttc ggtgttgccc
    25321 tgtattccga cgtggacatt atcagttccc tctgtgatct gatggcaaga ggcgctattg
    25381 taagtatgtt tgctgttatc ctgttcctgc cggccatgtt catgctcttc gacaagatga
    25441 tctgtgtcac cagcatcggc ttccggaaca agaatgcaga accctccaat acattgaaag
    25501 aaaggacggc ataagattat gatcaatttg aaaaaacaag cctggagaac cggggctctg
    25561 tgcctcgccg gtgtggtagc cttaagtgtt accggtgtct ccgtatatgc aaaaacttcc
    25621 gaaaagaaag agaccgaggt caaggagcag atccgggatg ccgtagatga catctggaaa
    25681 tccgaagata cctccgcagc ctccattgat gagaccgtct atgtgatcct gaatgcagac
    25741 ggaacccagc agaagatcta cgtcagcgac tggctgaaga atcagggaga aaaagattcc
    25801 tacacccaga gcaatcccga taaggatgct cccatttccc tgaaagttac ttatacgttg
    25861 gacggcaagg agatctcccc atcagatctg aacggccaga gcggtcatgt agtgatccgc
    25921 tatgattaca cgaacgaatt atatgagacc cgtgagattg ccggaaaaga ggaaaagatt
    25981 tatgttcctt ttgcggtaat gaccggtatg atcctggata atgacaattt cagcaatatt
    26041 aaggtgtcct caggtaaggt catcaacgac ggcagccaca gcgttgtcac cggtatcgtc
    26101 ttccccggac tgggtacgaa cctggatatg gaagaggatt tcgacgatta tctggagatt
    26161 gaggccgatg caacggattt caccatgaat gaatcctact gcatcgccac caattccgtc
    26221 ttctcccgtc tggatttcag taatgtggat gatctcgatg acctgacaga ggcaatggat
    26281 gatctggagg atgctaccaa caaactgttg gacgggactt ccgatcttta tgatggtgtc
    26341 acagagcttt atgacaaatc cggtgacttg caggatggtg taaaagaact gtccgatggt
    26401 tccaacgacc tccgggatgg cgcttacaaa gtagctgacg gtactgtcac tttaagaggt
    26461 ggcgtcggtt ctctgcggag cggcatcggt acactgcagg agggcaccaa gaccctgtta
    26521 gcaggtacac agagcctgca gtccggtact aatgatctga agaatggtct cggtcagctg
    26581 actgcacaga atgatacttt gaacggcgct gccggccagg tattcgatac tttgttgaaa
    26641 actgcagctt cccagttaac ggctgccggt ctcacgcttc cggaagaatt gacaatatct
    26701 aattatgcga ccatattaaa tgctctgtat cagcaaatgg agcctctctc ggatgctgac
    26761 gttgctctgg gatatgtcct ccagcaggcc ggactgacga ttgaaaaata tcagggtatg
    26821 attcaggcat cccagagcgt atcctcaggt gatgccgcac ttcaggcaaa tttgaaaacc
    26881 attcaggata aagccacaag tctttcacaa gctgccggca gcattgctgc cttgaaagct
    26941 tctctggatg gctataatca gttctacacc ggtctccaga cttacaccgc cggtgtagca
    27001 tccgcaagtg ccggtgtttc caaactggct gacggttccg cccaggtcgt tgccggtgct
    27061 tcccagttaa aggatggtgc tgacaaactg gcagacggaa gtaaagagct gtatgatggt
    27121 gctgatactt tagtagaagg tgccagagat ctggcctccg gcagcggcaa actggctaac
    27181 ggcgtaaacg agctttccga tggaagcgat aagctgatcg atggtatcag tcagctgaag
    27241 gatggttcca aggaattaaa ggacggcatg atcgaataca atgacgaagc catcagcaaa
    27301 ctgactgata tggataccga tgaacttcag gaagtgatcg atcgtatgaa agctacctct
    27361 tccgtatccg aaaattacaa cttcttcacc ggagacaata acggtatgga cagcagtgtg
    27421 aaatttatct acaagatcaa cgctgtaaaa taaaacatca caagcccgta acttgacaaa
    27481 gcccctctcc ttttctatac ttgcaaaagg agaggggctt ttcgcttcct atattccgct
    27541 tccgatagca gaaaggcata caaattccca tggaacaact attattcagt ctcaatgcaa
    27601 cgatccccgt attcctggtc atggtgatcg gttatgtttt acagcagctg catgttacca
    27661 acgactcttt tgtcaaaacc ctgaattcat tcaattataa aatcaccctg cccgtacttt
    27721 tgttcaagga catgtccact gcagattttt acagtgtatg ggataccggt tacgtgctgt
    27781 tctgcttctt cgtgaccctg ttctgcatcg tcatcacctg ggtcgtggca ggcatttttt
    27841 ataagaacaa atcccgcctg ggagagcttg tccaggcaag ctatcgaagc agtgccgctg
    27901 tcctgggtat cgcctttatc cagaacatct acgggaactc cggcatggct cccttgatga
    27961 tcgtgggtac ggttcccctg tacaatatcg ctgccgtgct gatcctgtcc tttaccggac
    28021 cggatgcaaa aggttttgac aagaaatctc tgatgaaatc cctaaaaggg atcatcacga
    28081 atccgatcat tatcagtatc gccctgggca tgctgtccag cgcctgccgg atcagttatc
    28141 ccgtgatcat ttccaagacc gtcagcaata ttgccgtact ggctacccct ctggccctga
    28201 tcggcctcgg tgccggattc gagggtaaaa aagccttaaa gcttttagcc cccaccggag
    28261 tggcaaccgt gttaaagctg gtaatatggc ccgctctgtt tctgccgctt gccgtgtatt
    28321 tcggcttcac cggagaaaaa ctggtggcga tcctcattat gctgggctct cccaccactg
    28381 tcagctgcta tatcatggca aaaaacatga accacgaagg aactttgact tccagtgtgg
    28441 tcgtagccac cactttttta agttccatca ccctgacggt gttcttgttc attttgcgaa
    28501 gcttgcagtt gatttagtta tatgatgccc acgaattgtt ccgtgctgcg cactctcaca
    28561 ttctacccaa tattcgcata tcgtgggatt atcatcccat ttttcccgca aggaaaatac
    28621 ttgccactgt tcccgctaac gttgccagca atgcccccgg cagcgtgtag cgggtttttg
    28681 ttacttttgc tgccagaaaa tacacgctca tggtgtaaaa aatagtctcg gtacagctca
    28741 tgaggatcga agtcagcatc ccggcataag aatccggccc acaggtctta aaaatatcca
    28801 gtaccagccc ggttgccgca gaggaagaga acattttcac aatgaggagc ggtatcagct
    28861 gtgccggaag tcccaccgcc tgtgtggcag gtccgatcag atgccccaac aggtcaaaaa
    28921 aaccggaggt cctgagaatc cccactgcca tcatcaatcc taccagtgtc ggaaggatct
    28981 ccacaacgat gcgcaggccc tctttggccc ctttcaaaaa agtctcatag accttcaccc
    29041 ccgagaccat cccataaccc acgatccaga aaatcaccat gggaatcata atatctgaca
    29101 tgtgtgtcaa tgtgttcatc atgatcctca cctgtccttc catttacagt aaatgatggc
    29161 aatgatcgta ctgaacagtg tggccaccag cgccggagca atgatcaccg ccggattggc
    29221 gctgccgtac tggctacgat aggcgatcat attgaccggg atcagctgta aggatgaaat
    29281 attcagaatc aggaaggtgc acatttcgtt gctggctacc gtggcctctg ctccataccg
    29341 ccgatcctcc gatccctgtc gtcccttctc cgtcccctgc cctgccctct gtttctccag
    29401 ctccgccagc tgctccatgg cctttaatcc agccggcgtg caggcccagc caagccccag
    29461 gacattggca atgagattcg tggttatgta ttcccctgcc gggtgatcct ccgggagcct
    29521 ggggaacatg aatttcagaa aggggcgaat cccttttgtc attttcgcga tcattccaga
    29581 ctcctgcgcg atctccatca gtcccatcca caatgccatg acccccgcca tggtgatgca
    29641 gaggctcacg gcctctctgg cactgtctaa cgcgcctccc gtcagtgcgc tcatctgccc
    29701 ggtacagaca ccatagatca cgcctaacag gatcattcct gcccagatat agttaagcat
    29761 ctcataaacc gcctgttatt ccttcgtttc actatatgcg ggcagcggta tgtcctatgc
    29821 ggcacaggtc acctttgcac gttgctcccg acgggatgtc acatgcaaaa tgagccttgc
    29881 gggcagtgcc ccctgccaga ttcaacgctt cttatcaggt gcactaaaat aaatctcccg
    29941 ggaataaatt ctccgggagg tttactctaa actacattta taagttatat ttattacctt
    30001 cggatctaca gagttccctg tgtgaacagg cttctcacat gtgccacttc ttctgcggtg
    30061 gctttctccg ccggcacata ataagacttc tctctggcga tctggtacag ttcctcctgg
    30121 gactgctcac aggccgtacg gaactgcttc agtgtctgtt ttaactcctt attctccgac
    30181 tgggcgatca ttcccgcata acgggtcagc tcgccgttga ttccggccag agtatctgct
    30241 accattgtct tttcctgcat tctgctgcct ccttactgca aaaacttcat aagctgctgt
    30301 ttgttctcca tcgcgctgcg agcacccttt tcaaaaaact gttttacctt cgggtcctcc
    30361 gcttccttgg cgtactcctg cattttgcag tgggtcgtgt caaaaccgcc gatcagatgg
    30421 cgcagattct gcagatctaa ttctgaaata tcactcatgc tgttaacctc ctttttctta
    30481 tttgcgaggt tagtatgcgc agaatatttt tttatattca ataaatatga tacctacgaa
    30541 ttgttccgtg ctatgcactt ccacaattct ccccactatt cgcatatcgt gggattatca
    30601 tccctctttt atgctcatat tggggacggg tcccaaggtc tttcacccag ccatgaacaa
    30661 gctcatgcgg ctttagacct tttaccctgg tatcattaaa taaaattggc aaatatagat
    30721 accgaaatca cggtcaaaat accgcagacc acgatggaca gactgctcat ggcgccttcg
    30781 atctcaccca gttccatggc ttttgcagtt cccagcgcat gagcactggt accgatggca
    30841 agacctttgg caataggatc ctcgatctta cacaggcgac agatcgtatc tgccagaata
    30901 ttacctaaga ttccggtgac aatgatgaca actacggtaa tggtcacaat accacccatt
    30961 ttttcagaaa tccccatacc aatagccgtg gtaatggact tcggcagaag cgttacatac
    31021 tgctcatgat tcagaccgaa catcaggctt aaagcaaaaa cgctcaccat ggcagtcagc
    31081 acgccggcga agattccgcc gaaaatggcc ttgggataag tctttaacag ctccagctga
    31141 cgatacagag gcactgccag gctgaccgtt gccggagtca ggaaaatgct gatatactgt
    31201 gcgccgtttg cataggtctc ataatctata tggaacaata acaatactgc cacgatcagg
    31261 attacggaga tcagtaaggg attggcaatg gcttttccgg tcttttgctt gatccacaga
    31321 ccgatctcgt aggtgaccag tgtaatggca atgccgaaaa atatggactg actcaatatt
    31381 tcattcattt atctgtcctc cttatcatct gcggcagctt tggttccgac cgtctgtatg
    31441 gctgcggcac ctttattttt ctgcccccgg ataatatgct gggatacccg gcctgtcacg
    31501 atcatgatca gtaccgtgga taccaccgtg atcacacaga tgggaagcag catttctttt
    31561 aaagcatcca cacttaccat gattccaacg gtggcgggca cgaacataac ggacatggca
    31621 tccacgagga aatccgctgc ggtctccacc ttttccagtg ggatcagctt cgtgatcagc
    31681 cccaccagca tgatcaccag accataaacg ctagctgcaa tgggaagcgg gatcaggatc
    31741 tccagaagct ctgccagaaa ggagattccc aggataatcg aaaattgttt cacatatttc
    31801 atgatattgt caccctacct gttaacttta gtattttatt tcttttgtta tatagcaaca
    31861 cgatatgata ccggggcatc attatattgc acacaagcaa aagaagctgc gcactatttt
    31921 ttcagcacgc aacttcgtat tatactactc gatttttact ttcacaatca gcattctcta
    31981 ccatttgagt agactttttg accttgcttt cgtgaatttt tcccttacat ctggtgtctt
    32041 accactgcat attcgtcatc cagtcggaag taaggacatt cctgctgggt tccggataaa
    32101 aagcggtaca tttcatcttc atccagattc accatgcagc aatagcattc atagtcttcg
    32161 tcatattcat aattgacaca agcgtcacaa ttagtcattt tccctctctc cgcatatgcc
    32221 tttcatggat gtatcctaca ttatcctcat atgcttttgt ctgttttgct tcggtttcat
    32281 tatggtattt tcactgtttc atgagttgcc gtgcaggtac tgatagattt cccgttttcc
    32341 gacacccctg tcttttgcca cctgcttcat ggcggattta ttatccatgc cctgctcttc
    32401 gtaataagcc atatgttctt caatgctcat ggattcccag gatgcgatct cttcctgtcg
    32461 tttctcctcc ggacttttcc cttccactac caggacatat tccccgcggg gctcctctgt
    32521 cttgtagtaa tccaacgcct tttccaaagt ggtggggaat acggtctcga acttcttggt
    32581 cagttcacgg cacaacgtga tccgccgctc ccccagtgcc gcatacagtt cctccagtgt
    32641 ccggatcaga tgatggggag cttcatagag gatcatggta cggctctcgg tcttcagttc
    32701 ctccaggatc attttctttt cctttttatc tgcaggcaga aatccttcaa agcaaaacct
    32761 tctcgtactg agtcccgaca aagtcagcgc cgtaatgcat gctgccggac ccggcagaga
    32821 agtgacggga acgctctgct cctggcacat ccgcaccagt acttcaccgg gatcggagat
    32881 cgccggggta ccggcatcag tgatcaacgc aatgttctgt ccgtttaaca gctgtcctac
    32941 cagctggtgc gctttttcta ctttattgta ctcgtggtaa ctggtcatag gtgtgtggat
    33001 gtcgaaatga ttcagcagct tgatactgtt acgggtatcc tccgccgcga tcacatccac
    33061 cgtctgcagg gtctccacca cccgcggtgt catatccccc agattgccaa tgggcgttgc
    33121 acacaaatat aaagttccgg gcatgagctc ctgcctcctc tcaaaatcat agtagtctat
    33181 aactattcaa agccatgata tcatgtcact atgtatggcg aatgctcgca ttcgtaagaa
    33241 catttgacat aattatacgg tgggtggtgt gctgaatagt tacaatatct taactatcta
    33301 atatccatat atatcatata tttccggcat atatactccc ggcgcctggt acacgatcag
    33361 tggcttttcc acggtcatgc gtgatctgcc gcctcgcacc gcctcgatga gcaccatatt
    33421 gggctccttg tcgatatagg gatataccag ctgcatccgt ttcggctcca gcttgtattt
    33481 taccagcatg gtcatgatct ccgccagccg aaaagnnnnn nnnnnnnnnn nnnnnnnnnn
    33541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    33601 nnnnnnnnnn nnnnnttcgg ctccagcttg tattttacca gcatggtcat gatctccgcc
    33661 agccgaaaag ggcgatgtac catgtaaaaa tgtccgccgg gctttaacag ttttgctgtc
    33721 ctgctgacca catcctccag cgtgcagagc acttcgtgtc tggcgattgc ttttggttcc
    33781 tccgggttcg tcaatccatg ctgtccgatc atatacggag gattgcaggt aatacagtca
    33841 aaggaagcag ccgggaaaat ctggtctgct tccttgatgt ctcctgtcac gatgtcaatg
    33901 cgatcctgca ggtcattcag tgccacgctg cgtctcgcca tatctgcgct ctcctcctgg
    33961 atttccagtc cggtgagatg agaacagtgg tattttgcct ccatcagaag cgggatgatc
    34021 ccggtgcctg tgccaaggtc aagcatcgta tccttttctc tggcatgggc aaatcctgtc
    34081 agaagcaccg catccatacc gaaacaaaat ttctccggat tctgtatgat ctgataaccg
    34141 ttccgctgaa ggtcatcgat ccgctctttt tccttcagtt caaccttcat tcgtattctc
    34201 cggttttctt ccgttaccgt tattaccgtt gttgccgtct cttctgccgc cgtgtctcgg
    34261 tcgtctgcgg aaggaattgt ccctctgagt tctgcgctcg ccttcctgac cgttaccacc
    34321 ttcctgttgt gtctggctgg cgggacggaa ctgatctgct ctctgtgcat tgtcccttac
    34381 ctggtccgga cggttctggg gacggttctc gcggcgtctg tcaccacgat tattgtccct
    34441 gcggttctca tttctgctgc cattgtcacc accgcggttg tcattgcggt tctgattctc
    34501 gccgttacgg ttattttccc cgcggttatt atttctgcgg ttgtcgtccc tgcgtccgtc
    34561 gcgtctgcgg ttttctccac ggttgtcacc acgattctct ccgtcaactc tctgctctct
    34621 gcggtcctga cgctccggtc gctgctcaga gcgcttttct gcgcgctctt ccgcttcttc
    34681 cttctcctcg atctgctcta acttctcgag ttccttctgt tcttccttgg acagttcctg
    34741 tccgctcttt ttgcctttgc gtctcttgaa ctgcaaagta tccgcctcgt attccttcag
    34801 ttccttctca tcgccgactt ctaccagtac cttgaccagc tggcgcagta cgttgacgct
    34861 ctgtacctcg ccgtgcagtc cgtctgccgt ctggacatag tcacccacac cgggaagctt
    34921 gcggttcagt tcctcgtaag tgtcttcctc atttttcaga cagcacatca gtctgccgca
    34981 gactccggag atcttcgtcg gattcaggga cagattctgc tccttcgcca tcttgatgga
    35041 taccggtacg aaatccgaca gatagctgtg gcagcacaga ggccttccac agatgccgat
    35101 accaccgcgg atctttgtct catcacggac accaatctga cgcagctcga tacgggtctt
    35161 gaacacactt gccagatcct ttaccagttc gcggaagtcg atacgtccat ccgccgtaaa
    35221 atagaacagc accttgttat tatcaaaagt atactccgca tcgatcagct tcatttccag
    35281 accgtgcttt aaaatcttct ccttacagat cttgaaggct tccttttcct tctccttgtt
    35341 ggcagcctcc tgctcgatat ctctctctcc ggcgatccgc atcacaggct tcagcggctg
    35401 gatcaccttg tcatcctcca cttcatgcac tcccgcaacg accgtgccga actcaatacc
    35461 tctggcggtc tccacgatca cgtggtcgcc tctctttatc tcaagctgca gcggatcgaa
    35521 gaaatatacc ttgcccgctg tacgaaatct tacaccaatt acttttgtca tatctatcta
    35581 cctcactgat gccgttggca tcttcattta aaataatatg taactattca gagccaaggc
    35641 aattttcata aatttgcgag gaatgccgaa taattacgat aacgttaaaa ataacagttg
    35701 tctgcgcaaa acgcgcctgc aactgctatt ttagcatatc tttggaaaaa tgactactgt
    35761 aatattatcg ctatattttg tcgcttttat atgtgtatga caattattta tattttagca
    35821 ttgctttcca tctgtatgga aaatccaagt gcttatttga tatatatttt ttatagttat
    35881 tcacaagttt tacaagtggt ttccatacgt tttcattcca cttctgatga tttggataca
    35941 taagttttaa catataaagt tgtgcaaaca gtcgccttgt tggaatatat ttctctgatg
    36001 acggcatttt ggggattgcc ggaaatatcc agtaatacag tcttgaataa tgtgcacact
    36061 tatttctaag atcattgaga catcgcatcc aactgtcaag tgtttggtag ttaatactat
    36121 aaagatttgc cgcaatagct gccttatcat tatttttcat tcccctgtaa aaatatgata
    36181 atgtgccaaa cgagaaaaat tcaataatta cccatattgg aaaatgtccg ttatatttct
    36241 tcatatgatg ctggacaact gttgtccttg cattctcttt tatgcaagaa ttaatccttt
    36301 ttgtgaaggc aatatggtca tgtttattat tataattaca agcatccata tatccttcct
    36361 ctccatactt atgtgcatga taatatgcta attgtgagcg aaaataaact tctatttcat
    36421 ccactactcc ggctataaga tttcgtaatt gctcatcaaa ttcatatatt gcctgaagtc
    36481 tttcaaatgt aatattggga aataatgaac cgtgtccatt gatttgaaat ggaagaaaat
    36541 atccagaaaa acgatagtag ttaacttgct ttagaaatgt ctttgcgctt acatcatcta
    36601 taatagcaat atttttgttc tttagtattg ctatctgctg atcatatgtc ttcggttttt
    36661 taataagcac tcctcctctt tagaaaaaat gtgcccccat ttgacacttc tatgagaggt
    36721 gtgggggctt ctggtaatat tattatatcc aaaatcatgg aaattgcaac tgaaaaatta
    36781 tagtttgact ctttttttaa cgcggattat tatgatattt ccatatatag tctgggcacc
    36841 acttgggtct atgcttttgg tataatgaca atgcaagttt ctgatataat tcctcacact
    36901 gatattcttt tggaagttca tcaatgtgct ttattgtata gtttgcgttt ttttctgcaa
    36961 tttcaataaa atatcgcctt atatgcgcct gttctttcat tcttgccaaa tatgatgcat
    37021 tttttattat tgctaattcg ccacaacttt tcacatattt ttgcagcaag tcatacgtaa
    37081 agtactttgc aggataatat ttcaaaaaat catcatccaa tttattcata agttgttctc
    37141 ttattatcct atctttaaac atgctatccg gtaccagtga cataaacagt tcaatctcct
    37201 catcctttgc acggcacaat ggaaacacct ttccgcccca gttttcatag ctaatatacg
    37261 gaaataaatt tcttttattt ctcccaagta ttattttccg taacattttt tctgttacat
    37321 cttctctccg taacagtaat atatttttcc agttatcctc tattagatta tacccactta
    37381 tctcattact tttaaattca atttttgagg ccacaagtgt attgtcccat tcgcgaagca
    37441 aatttgccga gaacaatcgt tgtacctgat ttactcttct agaaacagca tataagcttt
    37501 cttttctgtc tataaaaatc attttgtcaa tatattctga caagttttgc tgcggttgac
    37561 atatcaatgc attctctttc tgtaacactc ctaatatctt tttctgttcc tcaatttgct
    37621 cggctgtggt tatttgttca ctaagtcttt ccttattaat cgattctaat agtttgtcaa
    37681 atgatgcaaa ctgttttgca actccaaata caggagaata gtagtaggtt aacttaccat
    37741 catatggatt taccatgata tgattataat aattcacatc tacaatacat ccatgtattg
    37801 tgccgttaaa gccaagtcgt tttacttctg acgctattct ttcttgtacc aatctatatg
    37861 gctttaatcc ttcataaaat gactttacca tagataaaaa tgtctcatta taataatcca
    37921 tgctatttat cgtactgacc tgcttaacca ttcctcctac acacattttg actccttttt
    37981 gtgtctttac aaaaaaaaca tagtatccgt catgttttat caggtataag ccggcatctt
    38041 tttgctttaa aatattgttt atgagcttag gctcattcaa tctctctcca atatatctat
    38101 aataaagcac atattcctct atactaattt tgtggcatcc atccgtatat ctaatgaaag
    38161 gatcatacac gcaccccaaa ctacataatt tcatttgaat atttcccgta tatcctatct
    38221 gattatccgc aaaaattgtc ctatatttat cacgaacata tctgttgtga tgatcatgcc
    38281 cataaaaata tatacattgg gaatcttcct ctccttcttt taaccaatca tttacagggc
    38341 aatggctgat tacaatcaac ggcaagtgca ttttttttgc ttttaaaaga tattttttgt
    38401 atattgaata aaatatttct gactctttaa tctcttcatc cctgttgttt attaaatcct
    38461 tcgaatagca caagttcgta gtgttataca tttcattata tttggagaaa ccagttcctc
    38521 caaatataag acacttcgga acactacgtt tatctatata atcatatttc ataaaaccat
    38581 cattaacaag aaggtgaatc ccatgctttg cagaaagttt catatactgt tcatagcact
    38641 cttgccttgt agaataagca gacaattcgt gatttcccaa cacatataac actttttcca
    38701 tagatggaat ctctttatta agacacgaga aaaaaatatc aattaccaat tttcgaattg
    38761 ccggaggcaa agatcttttc tttatagttt caaacccttc aatgtgaaat tctagatgaa
    38821 tatccgaaat gtaaaaaata ttcaaactat cccaaatatt ttttacagga aattgtattt
    38881 cattctgcca cagcgaaaca tcattcattc accagttcct ccgtttgtac tatagcgcta
    38941 ctattcttcc ttcttgtaag aaattaactt aattgtagcg tcgtgaaata ccagaatcaa
    39001 taggaaatct ttcgtagata tatagttaga aactaatact gcctgacatg ctttttccag
    39061 tagaaaaatt tgtatacaaa aattaccaaa aaagcggcct ccttttacag ttagccgctt
    39121 tctaatatta tcattaggat ctgagttcca cacccagctt attgttcagg ttcttgagga
    39181 tcttcttcac atagccgtcc actgcctctg tggtgaattc ttcctccttc g
//
LOCUS       DAWDHD010000006        38120 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_92_length_38120_cov_13.167135, whole genome shotgun sequence.
ACCESSION   DAWDHD010000006 DAWDHD010000000
VERSION     DAWDHD010000006.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 38120)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 38120)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..38120
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92_length_38120_cov_13.167135"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6620..6719
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         9089 a        10128 c         8461 g        10342 t
ORIGIN      
        1 aaaactaaag gagaaaacaa atgaacaaga aagtactgta cctcgtacac ggagcggtga
       61 tcgctgctct ctatgttgtg ctgaccatgt tagccaacgc tctgggactg gcaaattatg
      121 ccattcaggt ccgcttctcg gaagccctga ccattcttcc cgtatttacc cctgctgcca
      181 taccgggact tttcctggga tgtatcatca gcaataccat gaccggctgt atgctgctcg
      241 acattatctt cggcagtctc gcaaccctga tcggtgcctt cggcacatat ctgctgcgtg
      301 ataagaacaa atggctggct cctctgcctc ccatcatcgc caacaccatc atcgttcctt
      361 tcgtgctggc ttatgtgtat catttagagg gtggcattcc ttatttcatg ctgactgtgg
      421 gtatcggtga gatcatttcc tgcggagtgc tcggcatgct gttacgcacc gtgttacaga
      481 aataccgcaa atacattttt gacgtacaga aataagcggt catcccgcca aaaaggcgtc
      541 cggatccttt cacaggttcc gggcgccttt tgctgtccac ttgtatttaa ttatctattt
      601 tcatatagga aataaaatga ttgccgttct ccgcgaacca tgttgtagca tcgttcattc
      661 cctttttgta caactttccg gtggacggct ccatgatgac aacgttgaat tcattaaatc
      721 cggtcaccag caccgcctcg ccatcctcca ggatcgccag taccggaata tcctgattca
      781 cataatacag taccgcatcc agactacagc cgctcagatc cagtacctgt acgcctgtca
      841 tattttcttc cagaatctgt attgccgtct tgccctgcgc caggtcatat tcggtgttgc
      901 gggtgattcc ggcatgccgc aggatattgt ccagacatac cgtcaggctg ttcttctggt
      961 ccgtgacacc ttcttccttg attgccatga tctggttcct gctgacacga ttgcccttca
     1021 gccataccgt aatgcctcgg tcgttcgcaa caaccccggc ggaatcgtag gcttccttca
     1081 ccgctttccc gggagcactg taaatgccct gtaccccata agcattgtat acataatatc
     1141 tggaaacttc gctgaccgca tccagtgtca gttctcttcc gccctcaaat acgacctcct
     1201 tcggattcag caccttgata gtcttggtat caatggttgt cttggtctgt atctgcacat
     1261 atctctcata aatatcaata tccgctgtga ctactttatt ctgtcccggc tcttcttcca
     1321 cattgttcat gatctggtca tccaggatct cctgatagct gccgttctca gaacgctgta
     1381 ttctcgacag ggtgatctga ttgccctcga tggcgcaatc cacgatgtag atgccatcct
     1441 gtccgtattc cttcagatta tctccggaag aattgctgat gcagacctta tacatagggt
     1501 aaaagatctg tccggaattc tctgtccgga tgtcgctctc cctggccacg ccgtagataa
     1561 tatcttctcc catgaaacca agcggccgga tagcctcacc gtcctccgcc cggatcactg
     1621 tctgttctcc ggtattcaga ttccggacat tcagctggtt gctgtggttg atgtcatccc
     1681 cttcctgcca tacgatgatc tcatggttct ccgatacctg aaggctgtca tcctgtctta
     1741 tacttaccag ctgttcagcg ctgcgattct ccagatccac atcatagaca ccgttttcca
     1801 ggaaaaaata tacatgttgc tgtctgttcc ggtacagcag ctgttccatc tcatccttca
     1861 gcaccgcata gctcttggaa tatgggatat agacttcttc ctcgatcgta ttcagcgaat
     1921 tgtcatagct gatgatctga atgccggtct ctccctcatg tcttccgcga ttcatgtaac
     1981 catacacggc aaatttcaca ttaccgccct cgtctacatc cagaatcttg atgccatgat
     2041 tgtcatacag agtccgccgg tcggcattat ctttatcata gaaggagaag atcactgtca
     2101 gtccgttggt agccgcatta tagctgagta actgccccat atcggagaac accacggtat
     2161 tgccatctgc gctctccatc atatccacat tttcatccgt gattcccaga aggatcttgt
     2221 cattggcata catccgggta gtatccggga tctgtgtcat ggtacgctca taatccagca
     2281 gatacattct ctccgaagta tatctgactc tgtaatactc ttcgacattg tagtagatct
     2341 tatttttgcc ctctccggtg gataccacaa aatccatcag cagagaggcc acattcccac
     2401 tgacctgtgt cagccggatg gaagccgcgt cctcctggac cggttccaga ctgccccagg
     2461 tgatctgctg aaagctgctg tgaatattca ctttatgaaa agtgccgtta tctgtcagct
     2521 tggaattcgg ttccagatat ttcgtcagtt ccttggccac ttccttgtcg tataattttc
     2581 cgtggaaatc tgtcgcaaat tccaggattt ccgaaatatg cacatcatca ttccataaaa
     2641 tagtcgtata ataatatacc ttctgttccc cttccagttc caggatcagg gtcagactgt
     2701 attgtgtatt cgtgtcgatc aggtctttta acgtaaggga aacggaaaag cttttcccat
     2761 ttgccttcca tccggtgact tcactgttct cgatcagccg gctgccgtcg atgctgcgaa
     2821 cctctgtcga gatccccgta atttctcttc cgaacgtatc cacggtgaaa ttcgcctgtc
     2881 tgccctcccc cagcagagta atgcagtcct tctggtaagc cacatccatc tccacggtat
     2941 ttccgtgcag ttcattgcat gccacaccgc cgctttccat ggtgatcata ggcagagtgg
     3001 ccggagccat ctccatggtc atattgttat gtccctgatt cataatgctg ccgatgatca
     3061 gtgctgatgc cagaaatact acaacacaca cggcaattct aatgatcgtt ttcttcattc
     3121 ttgattcttc actcattcat tattcagcaa tttttccagt gtcggtgcca catggagcag
     3181 ttcctccaca gtaccgatat ctgcgctttt cttcgggcgc ataccgttct ttttcactgc
     3241 ccggatcaac agattcttcg gggtatgttc catatcgatg aattccagga tctgggtctc
     3301 atagccctgt tgttccagca gatttgctct cagagcatcg gtgagcagtg ctgccacgcg
     3361 ctccttgatg atgccgtatt ttaacactgg ttttaaagga tcacatttta tctgccggtt
     3421 cagttcatgc tgacaacagg gaaccgccag aatgactctg gccccccatt ttaccgcctt
     3481 gtctaacgca taatctgtgg caaggtcaca ggcatgcagg gtcaccacca tgtccgccac
     3541 atctgtgccc tcgtaggtac tgatgtctcc cctctcgaac tttaatctgt cataccccag
     3601 cttttccgcc agttcattgc agtgttttat cacatcggtc ttcagatcca gtccggtcac
     3661 ctggatatct ctatgctgta attcatgcag ataatagtac attgcaaagg taagatagga
     3721 tttgccgcag ccaaaatcaa taatgcggat ggtacggtct ttcggcagtt catcgatcac
     3781 atcctcgata aattccagat accggttgat ctgacggaat ttatcaaatc tcgccctggt
     3841 cacccggccg tccggggtct gcacgcccag tcctaccagg aaatccaccg gttttccttc
     3901 ctctaaaata taatgcttgg tacggttgtg agccagtgcc tgcagttcct cctcttcaga
     3961 agcagccgcc ccctccgtcc gcgatgcctt gccactgttt ttcaccttca gtgtcagctt
     4021 gccctttttg ctgaccagag cggtggcctg cagggtcttc gctgagaatt ctccctgccg
     4081 gaactgctcc tgtaaggcgg tgcagatccg gtccgtcagc tgttctgctg taaaattctc
     4141 gtgaaatgcc tgagttcctc tgtaagcagt ctcctggaac aacagttcct cccggatcag
     4201 caccgggcgg atctttacct tctgggtctg ttccggatga cggctgttgc tcaggatgat
     4261 ctgctgcaga tctttattca aaattttctg taaaagtatt ttcagttctt ccatggttta
     4321 tcctgttttt caaatgtttt gcgcatatta tagcacgctt tcctataaga acgcaatcat
     4381 tccccgtcgt ccaccgtgtc tccttttgta gatctcattg cacaccaaca catggatcag
     4441 atcctttgat actttttccg cttcctgttc caggatcgtg ctgatcgtat ccgctatccg
     4501 gaacagactc aggcggtccg gatattcatc atagatcatg cttccttcat agtcgtattt
     4561 gtccaatatt tccgcaattc tgcgctgaca gcgtctgacc tctccgggat aggtctgctg
     4621 cagatattcc aagtcattta atagttcctt ttcatccatt cttccgggat atccgggcaa
     4681 tgtcataaaa aacggtcttg ctaccatttt aaaatgcctc atgctttttt ataacatatg
     4741 cattttctct ctataaagtg aaaaacaggt gctgcgaagc ttatcgcagc acctgaattc
     4801 tcgccattgc tctctgcaat gccgtctctg ccctggcaat atccgtctcc ggcgtcttgc
     4861 tgcgcagtct ctcttccgca cggtgcaatg ctgcctccgc acggttttca tcgatctcat
     4921 tcggccattc gataatctct gccagaatgg taactccttc gggaaggatc tcggcgaatc
     4981 cggaatgcaa tgctgcttcc ttctctccgt ccttctccgt aatatgcagg atacccggct
     5041 tcacgatcac cgtcagggga atgtggcccg gaaggacacc aatctgtcct tccgtggtat
     5101 tgaattctac catatccaca attccctcat agaatactct ttccggggtt atgatacgaa
     5161 gtgtgaattc tttatctgcc atatctgtac tcccctctca tgctatgcat gaacctttgc
     5221 cagtacatca tcaatgcttc cggcattcag gaagtagctc tccggaatat catcatgctt
     5281 accgtccagg atctcacgga atccacggat ggtctcgctg ataggtacat attttccttc
     5341 cagaccggtg aactgtcctg ccacgaagaa aggctgggac aggaatctct ggatcttacg
     5401 ggcacgggat acgaccagct tgtcttcctc ggacagctca tccataccga ggatagcaat
     5461 aatatcctgt aattccttat atctctgcag gatctcctgt acaccgcggg cggtctcata
     5521 atgctcctta cctacgattc tgggatccag aatgcgggat gtggactcca gaggatccac
     5581 tgcgggataa attcccaatt ctacaataga acgggacaga accgtggtgg catccagatg
     5641 tgcgaatgtg gtcgcaggtg ccgggtctgt caggtcatcc gcaggcacat aaactgcctg
     5701 tacggaagta atggaaccgt tcttggtgga tgtgatacgc tcctggagcg cacccatctc
     5761 tgtctgtaat gtaggctggt atcctactgc ggaaggcata cggcctaaca gtgcggatac
     5821 ctcacttccg gcctgggtga atcggaagat gttgtcgatg aacaacagca catcttttcc
     5881 gcccttgtca cggaagtact ctgccatggt cagtccggtc agaccgactc tcattcttgc
     5941 tccggggggc tcgttcatct gtccgaatac catggtggtc ttgtccaata cgccggactc
     6001 ctgcatctcg tggtacaggt cgttaccctc acgggtacgc tcacctacac cggtgaatac
     6061 ggaatatccg ccgtgctcgg ttgcgatatt acggatcagc tcctggatca gcacagtctt
     6121 acctacaccg gcaccaccga acagaccgat cttaccaccc ttctgataag ggcagagcag
     6181 gtcaacgacc ttaataccgg tctccagcag ttctgtgtcc gtagactgct ctgcgaaggt
     6241 cggtgcggca cggtggatag gctcgtaaga cactcctgcc ggtaccggct tgttatcaat
     6301 cggctgaccc agtacattaa atattctgcc cagggtattc tctcctaccg gtacggaaat
     6361 gggagatccg gtggcgatag cctccattcc tctcacaagt ccgtcggtag tacccatggc
     6421 aatacatctg acagtgtcat cacccaaatg ctgggagact tccactacca gttctcctcc
     6481 atctctggta gggatgcgga tcgcgtcatt gatctcggga agtttgccct catcgaagcg
     6541 tacatccagt acggcaccga tgacctgggt gatccttcct ttattctctc ctgccatttc
     6601 tacctctttc ctgcccactn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna
     6721 ttaaagcgcc tctgcgcctg caatgatctc tgtaagctcc tgggtaatgc ttccctgtct
     6781 tgcgcggttg tacttcagtg acagatcact gatcatatcc tccgcattgc tggtggcatt
     6841 atccatggcc tgcatacggg caccgttctc actggctgcc gcttccatca gtgcaccata
     6901 gatcagactg gatatatatt tcggtatgat catatccagt gcttccacat cttccggttc
     6961 gaaattcatc agcgcatcgg caccctctgt ggatgcaccc gcctctgcat catattccac
     7021 gggcagaagc ttcaacagct tcggctcgtg gctcaccgtg ttcttgaagt gtgtgtatgc
     7081 cagataaatc tcgccgatct ctccggctgc aaatgatgtc agcagtttgt ccgacagaag
     7141 cattgcttcg gcatatgtgg gctcttcgat gacctccgga taggattcct tcacggtata
     7201 tccctttctc tccagaatct cccggccctt attaccgatg gcatagacca caacgtcttc
     7261 tgccttccac tcctcctgtc cggtgatcag cttggccaca ttggagttgt aaccgcctgc
     7321 cagacctcgg ttggaggtga tgacaatgac cgcctttttg ggggaagccc ccggaacgag
     7381 atacttatgt tccagatgac ctaccctgga caggatattg tttacggtat catacataca
     7441 gtcaaaatac gtctgagaac gttctgcacc tgctctggct ctctgaagct ttaccgtaga
     7501 taccagcttc atggccttgg tgatctgctg tgtgccctga atgctggtct tacggcgctt
     7561 gatatcacgc attgatgcca tagttacctc tcattctgcc gtatatcacg gctgacacac
     7621 ttatttcaga aactctgctt tgaaatcatt cagtgctttt accagcttct cttccgtagc
     7681 atcggagatc accttttcct cagcgatggc tgcggggatc tcgggatgct tggtatccag
     7741 ataatcaaag aattccttct caaatctggt gatgtcctct accggcacat ccaaaaggaa
     7801 cctcttggtc accgcataga tgatgataac ctgatactgt accggcatag gctggtactg
     7861 aggctgcttc agcacttctt tgatccgttc gccctgtgcc agcttctcct tggtatccgc
     7921 atccagttcg gatccgaact gtgcgaagga agccagctct ctgtactggg caagctcggt
     7981 acggataggt gctgcgatct tcttcatagc cttgatctgt gcggcaccac ctacacggga
     8041 tacggacaaa cctgcattta ccgcaggacg gaaaccggag ttgaacattt ctgtctccag
     8101 atagatctgt ccatcggtaa tggaaataac gttggtaggg atgtatgcag atacatcgcc
     8161 tgcctgggtc tcgatgatgg gaagtgccgt cagggaacct ccgccgtatt cgtcgctcat
     8221 gcggactgca cgctccagca gtctggaatg caggtagaat acgtcaccgg gatatgcttc
     8281 acgtccgggc ggacgacgaa ggagcaggga gagtgtacgg tatgcagtag catgtttgct
     8341 caggtcatca tataccacca gtacgtcttc tccgttctcc atccactcct caccgatcgc
     8401 acaaccggaa taaggagcga tatactgtaa gggagccagg tcacttgccg tggataccac
     8461 aacggtggta taagccatgg caccgaactc ctccagtgtt tttacgatat tagccacggt
     8521 agatgctttc tgaccgatag ctacatagat acattttaca ttctgaccct tctggttgat
     8581 gatcgtgtcg atcgcaatag cggtcttacc ggtctgacgg tcaccgatga tcagctcacg
     8641 ctgacctctt ccgatgggaa ccatggagtc gattgccttg atacctgtct ggagcggcgt
     8701 gtctacgctc ttacgagtga ttacaccact tgccacacgc tcaatcttac ggaatttgtc
     8761 agtctgtacg gggcctttac catcaatagg ctgtcctaag gagttcacta ctcgtccaag
     8821 caatgcgtct cctacgggaa cctctaccac acgtccggtg gtcttgacga tatcaccctc
     8881 ttcgatattg ctggcacttc ccaacaatac gacaccgaca ttatcttctt ccagattcag
     8941 caccatgcca tatacatcgc cggggaactg taacagctca ccctgcatgg cattttccag
     9001 tccatgtaca cgagcgatac catccgctac ctgaatgacg gttccgacat cggatacatc
     9061 cagctgggaa gaatatcttt cgatctgttc cttaatgacg gaactaattt cttctggtct
     9121 taaattcatg gatctgtcgc acctttcttt cagccggtca tcccagctga atatttaata
     9181 attgtttctt catatcttcc agtttggtcc ggatgctgct gtccaccacc cggtcaccca
     9241 cccggatcac cattccgccg atcagcgtcg gatccacatg gtaatacaca tcaaaggtct
     9301 gataggaagt cgtagccagc agcttatttt tcactgcctg cttctgggcc tccgtcagct
     9361 ccactgcggt atccacatat gcggttccca tcttttcaga ttctctgacc agttcggtaa
     9421 aataagcaaa gattgccttt aatgagccgt aacgttcctt ggtcaccacg atcttcagaa
     9481 actctgtcag atcatcactg actctgccac ggaagacctg ttccaccatg gagagttttt
     9541 cctgtttggg gattccggga tgtttcatca gcttgtcaaa atccggattg tcttccaata
     9601 ttttctcaag ctgtgcggtc tcattcatca gctggatcgc atttccatgg tcgactgccg
     9661 cttcgaaaag ggcttctcca tagactttgg aaactaattt tgccatgtat cctcacccat
     9721 ttccttcagc gtctcttcga tcaggctgtt ctgtctggtg gtatctatgg aagcgcctac
     9781 cagcttacct gccatgacag atgccacgga tacgatctcc tgtttgatct catcggacat
     9841 tttctgcttt tccagtctgg cttcttcctg tgctctcgcc aggattctgg ctgcctcttc
     9901 cttggcctgt gtgacgatac gctcctcgtt ctgaagagcc cgtcttctgg catcacttaa
     9961 gatgtcctcc gcctctttgt cgatgtcttt caacttcgcc tcatactgtg ccttcagttc
    10021 tgcggccttt tcctgattgt cctttgcctc ttccagttct cccctgatct tctctttacg
    10081 atcatttaag aactttctgg ccggattgaa cagaaaatag ctgagtgcca ggaacaatac
    10141 gaagatggca atgatcatca ggcaggagtc tgccagcagc tgccagtcca ggtcaaacag
    10201 tcttgacatg gtctcgcctt ctgttaatag tatcattttc atccgcattc ctttcgttta
    10261 atcagggaag taagggcttt acgaacagca gcagcatagc gatcaacaga ccgtacagac
    10321 cggtggtctc ggcaactgcc tgtcccaata acatggtaga agtgatctcg gactttgctc
    10381 cgggattacg tcctactgct gcagcagcgt gtcctgctgc gataccctgg cctacaccgg
    10441 gtccgatacc ggcaataact gccagaccag caccgattgc agaacagcct aagataaagt
    10501 tttcattaaa atccatagta aatcctccta ttattgagat tcttatcaga atctttctag
    10561 catagtttca gatagccata cctgaacagt acagctttaa gcgtctcctc ttgcattggc
    10621 aatgtaagtc atggtcagca tacagaatac gtatgtctgg atggctccgg agaacaggtc
    10681 aaagtataca tgcagtgctg cgggccacgc tgtagcgatc acacccaaca gtccgtagat
    10741 cagtgccatc attaccgttc cggacaatac gtttgcaaac agacgtaatg aaagggaaat
    10801 cggtactgcg atctcactga tcaggttaat gggcagccag atcggcagcc aggggggcag
    10861 cggggaacac atgccttccc agatttcctt cggcttctgg tacttgaact gattgtaatg
    10921 gatcagcagg aaggtcagaa caccgagagg caatgtcacg ccgtagtccg ctgtgggggg
    10981 acgcaggcct aacagaccgg aaatattgct caccagaatg aagatgaaga tcgttccgat
    11041 gtagttgtag aacttcggtg cggccttgcc catgggagca tccaccatgg catccagctt
    11101 ctccacgatc agctccacca cattctgaaa accttccggc acttctctgg ctttggccat
    11161 ctggcggttg gcagcaatgg caaaaccaat gataagcaac atgacgatca gcacacacac
    11221 atgtgtggtc gttatccata cctcatggcc gaaaaaggaa tacttaaaca cgccatgtat
    11281 cataaagtcg atattgtcgg caccggataa cagaattcca tgttccattg tttcacctcc
    11341 ttattgttta ttctgttgtt cctctgtcac ttctccatcc ttcggggctt ccgcttcttc
    11401 cgcagtctgc ggagcagttt tgtcttccgc agtctcctcc ggcatcgcct ggggtatcgg
    11461 atccgtctcg tggaacagtt tattgcataa tttatgggta atcggctgta aaaatgccgt
    11521 tactttcata catataacat aggtgaaaaa gaaaatcaga ggattcatca ctccggtctt
    11581 catcatcacc aacaggaaaa aaatcaacat gacatatcgt aagacatagg cacggatgat
    11641 cattttcgta gcagtcttct cgggctggtc caatgcccgg tccagggaac ggtacatatg
    11701 atatgtggaa accagcgcca gcagaacgcc aaaccacaga cttttggcat acattgcttc
    11761 atcctttgca ataaatgctc ctatgatctg cgctgacact cccaggaaca agatccccag
    11821 atccatctcc agaaccgttc tatttatctt ttttagtgtg tttatcatcg ccgtcttcat
    11881 aaattgtcct ggccatccgg tatacattct gccctccggc cacagctccc atacagaaaa
    11941 gcaggatcat ccagaagtcc gttccgaaat gtttatccag ccagatgccg gctgcggaca
    12001 tcaggcagat cggtaccaac atattgatac caaattgcat gatcagtgtg atcgcccgaa
    12061 acacctgtct gtcgtagggt ttcttttttc ttgacactgt gtcctcctgt gttgatttct
    12121 gctctcagca tttatttttg tacatttttg aaaatatttt cccactgcag ggcaaaccta
    12181 tagtcattat aacgaaatct taatatgatg tccagtaaaa tcctcctgtc cagatggtaa
    12241 aaaatctata aaatcctgcg aaaaaaagat tgacttgaaa tctctccgga agtactattt
    12301 gaataactat tcagtgactt tacaccctta tagaaagaag gatgacgcga ataaagacta
    12361 cagacatcaa aagacagctg cggatccgta tcgccacccc tgaggatgct gcaaggctgg
    12421 ttgccattta tgccccttat gtggaaaata cctctattac ctttgagtac atcgttcctt
    12481 ccgcagaaga atttgcggac cggatccgtc acactcttac ccgttatcct tacctggtgg
    12541 ctgaactgga cggggagccg gcaggatatg cttacgcatc cgcttttaaa agccgcgctg
    12601 cttatgactg gtctgtggag acttccatct atgtcagccg ggatcttcga agcagcggtg
    12661 tcggcagtct gttatacgaa aagctggagg aatacctgac cgcacagcac gtctgcaatg
    12721 tctgtgcctg catcacctac cccaatcctc ccagcatcgc cttccacgaa aagcatggtt
    12781 ataaaaccgt ggcacatttt cacgcttccg gttacaagca ggatgcatgg catgatatga
    12841 tctggatgga gaagacgctg tgtcctcaca cgatccctcc caggcctttt atcccgtttc
    12901 cggaactgtg accgttgtaa agcacaccgc acaaaggaga aactctatga gcaacgaact
    12961 tcagatctac cgtaaacggc tgatcccgga agaatgcatt ttattaaagg atgatattat
    13021 cgtcgagcag aacgaagact acattctgac caaatggaag accttgaatc ccaagaccac
    13081 gttttcccac ggctgttcct gctattatct caaggaaggc ttcaagatca gcaagttcta
    13141 ccgccatgat ggttctctgc tgtactggta ctgcgatatt gtggaatata cttcccggcc
    13201 ggaagataat tccctgatcg tcacggatct gttggctgac attatcctct atcccgatgg
    13261 aaggatgcat gtcgtggatc tggacgagct ggccgaggct ctggaaaagg ggctgatcac
    13321 ccaggctcag atgagtgcct gcctgcgcca gctgaacaac ctgatcacca ttatctaccg
    13381 ggacaagttc gacaggctgc agaatccgtt ggataggagc gggctgtagc tgcagtgctt
    13441 actttttgct ccctggtcag aactattcag agtaacacat tcgcaaaata aatgagagca
    13501 taggctctta cggacacaag tgtccgacga gcttctgctc tcacttactt tactctgaat
    13561 agttcagcag gtttccgatg cagtgtccca gaatcgtcaa cagtgtaata tcttcatccg
    13621 accacttacg gttgcggttg aacacatcat agttcacgat cacattcggc actccctctt
    13681 tgcggcaaag acattgcagg aaagcaccga tctcctgctt ctgcatcacg gcaaatgccg
    13741 ccggatcctg ggatctgagt ttcaatgtgt tggattctat aaaattatca tcatccccga
    13801 atagcgcaag atacttctca tcttcaaaaa gtcctcttcc atccggtact tctaccatgt
    13861 aattaccatt ccggcatacc agctgcgctc ccgactctga ataaatcgtg attccatcca
    13921 gatcgaagag actgcggatc tgctgcagac gttcttcctt ttttacaatg gagttcacac
    13981 cggatacaca cagttcggtg atcatcttcg tgatgacttc ccgctttttc tccttagata
    14041 ccgcataggc catagccatg ttctgcatat cgtcgctttc cagcttgccg tgaagtctct
    14101 cttcatagat gatatggcgg ttcttgcctt tttccttggc cagatagagt gccttatccg
    14161 ccttgcgcat cagttcctcg tattcgcttc catctaccgg atactccgat acgccgatag
    14221 aagctgtcac atgggtcttc ggatcaaatg ccagagcaaa ttccttcgca atggtcttca
    14281 aaagtgtctt cagctgttct ctggtctcta ctttctccaa caggatcaca tactcgtctc
    14341 cgccgaatcg tccgatcacg ccccgctgtc ccaccagcac ctgcatgatc tccgccactc
    14401 tgcggatgac ctggtcacca aaaatatgtc cgtaggtatc attgatatgt ttgaaatcat
    14461 caatatcaat gatcaccagc catctggtac tatgatcctt gcagttcagc tgttcgatgg
    14521 tgtactctgt ccatgcccgc ttgttcagca gaccggttcc ggcatctctg gctgccggag
    14581 tcagataata ggcttccttc tcccggtttc tgtccggggt tatgatgccc gctaccatat
    14641 cacgcttctt cgggatccgt gcgcctttga ccttccatat tcccttgtcc tcctgcccgc
    14701 tgacgaattc catctcgaag aaatcgtccc cgttacgcag atgtgcgcaa aaggtccgca
    14761 gctgatcttc cagtctggaa atatcactgc cctcatgtgc ctgttccaca ttccggatca
    14821 gcgcattcat aggcccttca aactggttca tggcccgctc gttgacatat ttatagaccg
    14881 taatactgtc ctgttcgatc agatattcaa aaaagtaaac atcttccaat gtcataaaat
    14941 gacggtactt cagaatgctc tgctccagtc gtaaattccg gttctccaga tcatgaatat
    15001 ctgaaacggt gatctgatac aacggaatcc cgttctccgt ctgttcactc tgttccagac
    15061 gcagatacac attccttaca ctgtccttca gattgtgaag caccgtgatc agttccagcg
    15121 gttggaacgt atccgcctcc actgcttttt tcaacagctc cccgtcttcc ggcagaagca
    15181 gctcactcac cggccggaaa gaattctccc ctacgaattc atagaaaaat tccgacgcct
    15241 tggctactac cagatcctgc cttacgaaga tcttccagtc catgattcta tttgcataac
    15301 ccttttgttc tacgctgttg agtctcatag attttcgctt tcgtttaata aaatacgtgt
    15361 ccgccgatat tgatacctgt cagtccttcc accggtgtgc ggaaatatac acagtttcct
    15421 acattggtca caccggacat agcctcgtct gccgcctggt agcagcggct ggtggcttta
    15481 ttggtggcca atgccagttc cagacgtccg ctggccaccg gtgagaactg tttattctga
    15541 tagatcactc ctaccactgt gttgggataa acagaacttc tgacacggtt gataaccaca
    15601 ctggctaccg ccagctgacc gtcatagggt tctccgcccg cttcacaata gatcagattt
    15661 gccagcagat aacggtctcc ctcttcaaag gagatatccg aaatgtcccg ccaggttgcg
    15721 tttgccgcct tctgggacat ggcgatctct tctgccagct ttttccggag tgtttccaga
    15781 ttttcttctt tttgtttgat ctcctgttca tatgccaggg ctctctgctc tgcttccgaa
    15841 atatccccgg catattttgc aatgctgtta gatgtctggc tgatcagacc ggacaccttg
    15901 cttttctcgg cttccgctgc caccttcaga ctatccagct ccgttttctc tgcctgcagt
    15961 ctcgcctctt cctcttcgat cagcttgcgg ttctcttcga attcctgcag tttcttctgg
    16021 tcataggctg cgatccgttc aaaaaagtcc gcagcgttca gcagtttatc gaagctctgg
    16081 gcattcagca aagcgttgat gtaactggtc tcattccgct cgtacatatc ccgtacccgg
    16141 atcaccatac actcatactg ccatgcttcc gtagctttcg cctcttccag actggcctgg
    16201 gtatccgcga tctcctgctc cttgtctgcg atctgctgtt ccagatctga cagattgtta
    16261 ctgacttccg tcagctgacc gttcagatta ttcagttgtc ccttgaggga attctgttct
    16321 cccttcaggt tctccagatt atcattttcc tcattcagct gattctgcag gttgtttttc
    16381 tcttcctgct ctttctgaat ctgctgccag gtactgctgg tagcttccac tttgatatct
    16441 tctgaagaaa aggctcctgc aaggatcact gctgtaagca gcacagctgt cttctgtagg
    16501 cgcttcaacc gttttactcc ttttatctgt tgcttcagac ctcgatgcgt atcatgcggc
    16561 ccgtcttacg gtactgcacg taattcactc cggccgcgtc aaacatccgc ttggctgccc
    16621 ggttggacgg agttcccgca tacttatcgc tgtcatatac caccgtgcgg attcccgcct
    16681 ggataatggc tttcgcacat tcattgcagg ggaaaagaga gacatacagc ttcgtcccct
    16741 ccagggaacc gccacgataa ttcaaaatag cattcagttc actatgtgtt acaaagggat
    16801 actttgtcag attcctgtca ttgtcgttct ccctctccca ggggaactca tcgtcggaac
    16861 aacccttggg gaagccgttg tatcccatgg acaggatctt gttgtccggg ctgacaatgc
    16921 aggctcccac ctgggtattc gggtccttgg accgcattcc agacaggtgg gccaccccca
    16981 taaaatattc atcccagctg atataatcgc tgcgtttggc actcatgggt gtctccttta
    17041 tttggtaccg aaaatacggt ctcctgcatc tcccagtccg ggaacaatgt atccatgatc
    17101 attcagcttc tgatccagtg ctccgatata gatatccaca tccggatgtg cttcctgcat
    17161 agcttttaca ccttcgggtg cagcgatgat gcacagcaga cgaatgctct tgcaaccctt
    17221 gtcctttaac agctggatgg ctgcgatgga agaaccgccg gtagccagca tgggatctac
    17281 cacgaatacc tcacgctctg cacagtctgc aggcagcttg cagtaatatt ctacaggctt
    17341 taaggtctca ggattgcgat acaggccgat atgtcctact tttgcagcag ggatcaggga
    17401 taacataccg tctaccatac ccagacctgc acgcaggata ggtaccagtg ccagcttttt
    17461 accctttaac tgcttcacag tggtcttgca gataggggtc tcgatctcta cttcttccag
    17521 aggcagatcc ttggttgctt cataaccgat cagcattgcg atctcgctga tgttctgtct
    17581 gaagtccttg gtaccggtct cctttctgcg gatgattccg atcttctgct cgattaaggg
    17641 gtgatccata actactactt ttgacatatt gttgtcctcc tgtgttttgt atttgttata
    17701 atggtatttc gttgttcgtg tctgtgtaat gcattaaacc tcttcgatca ggctgattct
    17761 gcggctgtgt ctctcttctc cggaaaaagg tgtattcagg aaagtctcca cgatctccag
    17821 cgccaggttc tcaccgacaa tgcctgcacc cattgccagc atgttggcat cgttgtgcag
    17881 tctggtcatc ttcgccgaca gaggatcgga gcagagtgcg cagcggatcc cctttacctt
    17941 gttggcagaa atgctgatac cgataccggt ggtgcagatt acgatgccct tctcgcaggt
    18001 accgtctgcc acagcccttg ccgctgcatg tccgaacaca gggtaatcgc agcttgcctt
    18061 gctgtcacat cccatatcct gataaggaat gttcttctcc tctaaatatt tctttactct
    18121 ttctttcaga tcatatccgc catgatcact tgcgattgct atcattttcc ttctcctcgt
    18181 attttctgtt taaagtttta ctactttatg gcccgctgct ttcaacagcc ggttcataat
    18241 ggcctgtccg aagccttccg tggaaaatcc ctcggaatag atctttgtca caccttcatc
    18301 atcgaattct ctcagaatag tgtacagatg tctggcgata ctgtcctcat ccttacggct
    18361 gccgacactt ttcaccacat ctgctatata accggacagc atttcttcgg ttccgatgat
    18421 tccggtcttc tctcctgcgg tatggtctgc tgccgcagcc cggttaatat actcccttac
    18481 ctgccccggg gttccctcta cgatggtgag ttctcccctg ggtgcataat gtctgtactt
    18541 cattccgggg gccttgggtg cctgtccgct gtctgcccgc aggatcgtgg tgtccgtatc
    18601 cactgttccc agcactgtgc tcagtttctc ctgggtaatg tatccgggcc gcaggatctg
    18661 tggcggtgtc accgtcaggt caataatggt ggattccaga ccaatcccca cctcaccgcc
    18721 atcaatgatg gcatcgatcc gccccatcat atcttcttcg acatattttg ccactgtggg
    18781 actgggcttg ccggaagtat tggcactggg cgccgcaata tatcccccgc tgtatttgat
    18841 cagcttctgt gccgccggat gcgagggcat acggattgct accgtatcca ggccacctgt
    18901 ggtctcgtaa ggcaccgcgt ctgatttcgg caggatcatg gtcagggggc ccggccagaa
    18961 ggcatccgcg atcttccgcg cctcttccgg tacctccttt gtaatcttgt aaatgtcctc
    19021 aaaccgataa atatgaacga tcagtgggtt gtcggatggt ctgccctttg ccgcatatat
    19081 tttctgggag gacgaaggat tcagtgcatc tccccccaac ccgtacacgg tctcggtagg
    19141 gaaagccacc agtcctccct gctgcaggat tgctcccgcc gcccggagtt tctcctcatc
    19201 ttcttcgctg acggggccct ctccgggacg aatctttatg atctcagtct ccatgttttt
    19261 accttctgtg ttctaaattg catttggata tagtatatca tatccccggt caaatggaaa
    19321 gattattcct ctccggtctg ccgggatgtt gtgtgtacag aataaacggt ccatccgacc
    19381 agcagcggaa tcaccgccac ggtcggcagt tcatagcgga ttgcattgga catgggggat
    19441 gccaggcaca ccaacacgtt tataaatccg ggaaccagca gtatcaacgc actgtatctt
    19501 ttctttttcc acaggaaaaa tgccagcact accagaatcc aggtatacaa tcccggagta
    19561 cacagatact tgatcagcgg cagccgcata ctgaggaacc agatctgcag cagaaactcc
    19621 gatgtctgaa tggggaatgt atgatgaatt ccctgttcct gcgcatattc cggatactcc
    19681 agctggatcc aggcctctat gttctgttcc accggtgcca gatagccata gcccttgttg
    19741 acaaatgccg ccacatagca tcccggatgc ttgaaaaaca tctgcaccca tattttcatg
    19801 tagggcccca ggtctccgcc tcgatataat attttaatgg gatccgaaat ccggggttca
    19861 taatttttca tagcatcaaa gttcagggta tcattgatca cctgtctctc atattccgta
    19921 acctcatctt cgtattcgca gacatatctc gccgtctgct ggaacggtac gctcagcccc
    19981 tcggaagctg ccggttttcc cacatcgatc aacgctctgt atcccaccac cgtgatcagt
    20041 tcatagatca ccacagttgc cagcaatacc aggaatgcct tcctgcgttc tttgcctttc
    20101 agataaattg ccagcaaaag cagtgccgga acaatcgcat agatcccgtt atttctaagc
    20161 aggcaggata atacactgaa tacaatcagt cccgccgcat ctttcccgcc acattccttc
    20221 tttttcagta ccgacatcag atatactgcc tgtaacaccg tcatttccgc atacagcaga
    20281 tctttctcaa accattgggc ataggttccc caaaaaggtg tcagggcgaa aaacaggatt
    20341 cccacggcac accctttcca tgaaattccc agttcctgca gttttttcat gctgagggaa
    20401 aaagcatagg cccctaccag tgtctgcagc aggcaataca ggaaaaagcc gaaattggca
    20461 tctgcaatgg ttcttcccag cacaaaacag attcccataa ttgcggagga aaatggcgga
    20521 tgtgccgctc cccagtcctg ttcacccaga aactgtttca gttgcaccag accgtcccca
    20581 ctggtagttc ccggataatt tagcaggatc cagaacagcc agctgaaggc tattacaaaa
    20641 aaacccacca tctgtaggtg ctttttgccc tgctctgcgg gtaattccac atgctccagc
    20701 ccaaaatata ccaggcgcaa taccagatac aataacactg caaaccccaa aacacagaac
    20761 gtggataata gtatctgaaa agaattgccc cacaggaaaa tggcagaatc atatttctta
    20821 aagctgatac atactataag aaggaatgac agaataacag aaagcaccaa cgttgccaca
    20881 tctgtcttcg cctctctttt cttcacttcc ttaagcatta caaatactgc cgcagccgtt
    20941 aacagatctg tataatccag caccgtaaat gctccgcaca gtttattcag cccattgaca
    21001 agattcgaat ccacgtatcc tcccaggcag acttcaaatg tacattgcaa tgccagtgtc
    21061 acgatcacgg tcaacagaac atttcttatt ttttgatatc gtctcatttt ctctcccgta
    21121 actttataaa attctgtcat aacattcgaa tcctatatta tcataataac actgtgtata
    21181 caactcgtgc ctttattttg tatttcccgt ttcaatccct ctgtatcccg ttaatgtatt
    21241 gtaaaattcc tttgtggatc gaccatgcca ctctgtcctg ataggcctcg tcacacagtt
    21301 ttcccgcctc tgccgcattg gacaggaagc cgcattccac aatgacgatg gttcctttgg
    21361 tttttttcaa aagataatag ctgtcgttgg gcttgatctg ccggtggttc tccggatcca
    21421 gatcctggat cagctgtgtc tggatcagcc ctgccagttg ttcccctccg ctgctgccat
    21481 tataatagaa gacctgcgct ccgtgcacat actcttccgg atagctgttc tgatggatgc
    21541 tcaccgtgag aaccggatcc gtctgctcaa tcttttcgat ccggcgtttc atatcctgca
    21601 ctttcttatt gggagcagat gcatcataca atccatcctc cgtctctctg gtcagcacca
    21661 cccggattcc gtctgcctcc agatactgtc tcagtttctc tgtgatccgg agattgatat
    21721 ccttctccgg tgctccgttg ataccaatct tccccgggtc atccccgcca tggccggcat
    21781 cgatcactac cgtcggtatt ccgtccgtgt ccgcattatt cccccgcaag gcattcacgg
    21841 catttaccgt ttccacatat gccgccgttt ccctgccaag tatcgccata gacataagca
    21901 acaggcatac catcaccgcc cttaacactt tctgttccat ccctgttctc cggtgtctct
    21961 tttattctat atgattttat atgccttttt ctctggaaat atcttccgaa aattaagaaa
    22021 tccttttgtt ttaaactatt gcatcccctt actacttcag cgtataatac cattatgaaa
    22081 ataagcaaaa aaactctttc tatcatttta tttgttctgg cgggaatttg tgttcttgct
    22141 gcagtgatcc tggtggtaaa agggcggatc gaagaccggc gtatggagga agcgctggat
    22201 gaattgcgcc cgacgcagtc cgcggagacc actcaggcac cttccacagg cgaaggtgag
    22261 ccgaccgtta ccgcctcccc ggaaccgtcc acagaagaga ccccggcttc caccgaggag
    22321 ttcgaacgag tggccaaccc ttatgccgac agttttcttg ccaacaagga tatggcggcc
    22381 tggctacaga ttccgggcac caatatcgat tatccggtaa tgtggactcc ggaagatgag
    22441 acctattatc tctaccgtgc ttttgacggc agcgaaaata aaaacggctg tctgctcctt
    22501 gacgatgaga gctgtgtaga tcctctgacc acgaatctta tcattcacgg tcacaatatg
    22561 aaatccggtg ccatgttcgg caatctgacc gattacgaga atcaggattt ctataaggaa
    22621 cacaaaaaca ttatcctcta taccgaagag tgtcagcgca attacgagat cattgccgtc
    22681 tttcgctccc aggtctaccg gaaaacagat caggtattta agttctacaa atttttccag
    22741 gcggatacgc aggaagaatt tgatgacttt tataacaata tcaagcagtt atcccagtat
    22801 gacaccggtg tcaccgcaga gttcggagat cattttctga cactgtccac ctgcgtatac
    22861 catgtagagc agggccggtt cgtagtcgtt gccaaagaag tggaacccgg tgatcactac
    22921 cttcccatac aggaataact cacagacaat caggaggttt tcttatgaag agacttaaaa
    22981 aatttgtcac actgttcacg ctggctgccg tatgctttgc tattcccggt ctgggaaaga
    23041 tcactgtaaa ggctgcggag cccaccacct atgttctgaa ctattccgac agcagttccg
    23101 agtggagata caaggaagca tccagctggt ccgcagaagt tcaggaccgc gagttgtatt
    23161 atctgcagca gaatatcaaa gacggtgact atattgtcat cgacaacgat gtggagaaca
    23221 atgccctggc cctcaaggtt tctgtccgtc tgggcaacct gaccttcaag aatactgttg
    23281 gtgttcccgt agtctatgcc aacggctatg acagtgtcta tttcctgagc ggtacctccg
    23341 gtgctgtcaa cggtgatgta tctcatgcct atgtatatgg cgatgccaag gcaaatttca
    23401 acagcaatgt agacactctg gaaatgatcg gcctgacaga tgataaatcc aataatctgc
    23461 atgccaccat caacggtgtc ggtaccgtca atcatctgat tgccaaagac aaccgtgacc
    23521 agagcgtttt ctatgaggcc tacagtttcg ccgcaggtaa gctggccatt gaagacggat
    23581 ccctgaagac cgaagcaaag tattacagca ccaccgctcc cgctcccgct gctgccgcag
    23641 aagcagctcc tgcagctacc gagcagccca cacagaccac agctcctgca caggccacac
    23701 agaacagtac ttcttccgaa tatgacaacg ttccaaagac cggtgaaaat tctgcaatgt
    23761 tctggcttct gggtgccgca gttgtatgca tggccggagg ctataccatc cgcagaaaga
    23821 acgtacagga agcggaataa agttttcggc agcaataaag cccagtccct tgcgggaccg
    23881 ggcttttact attttattct gttcctactg cagataggca ttgatcagtt cccaggctgc
    23941 ctgtgttccg taacctagtc tccataccgc cacaccacca atattcttgg tagccatcat
    24001 gttcagcttc actgcgatgg attccgcatc ttccagccag atctgatagg tggcatttcc
    24061 ggaggtccat tctgcataat tctgacaggt ctcctcatcc cactctgccg tcacaccggc
    24121 tctgttcatg aagtctgcct cgttgttcat ggtaatatat tcatccgtca catccgtgcc
    24181 ttcggtcttc cacaggatcg tgtagaacgg aagggcattg actaccttgt ttgcaggcac
    24241 ctcctgcaat gttctgtcca gaccgccgga tacatagctg atgcttgcca cgcttccggg
    24301 atccttgctt ccgtgccagt gttcgtcata tcccatgata atgacatagt ctgcaacctg
    24361 tccctggata tccagacggt aataatcatt gaaattaaac ggtacatagt tggcaatgga
    24421 caataccagt cccctgttgc ggcactgaat ggaaagttct cgtataaatt ccacataatg
    24481 cggtccgcag tcactgctca gctgctcgaa gtccacattg atgccgtcca gtcccaggct
    24541 gactgctgca tctgtcacat tccgcacaaa gttctgccgg ctggtggtgg aggacagcat
    24601 attcagagta ctgatggccg ttccggtctc attggcgtaa ttaaaattat ccaccacgcc
    24661 ccatacctgt aagcccatat tatgggccgt ggttacataa ttcgctgttc cgaagttccg
    24721 gatacttccg ttctcatccg tcagactgaa ccaggtcggt gcaatgacat tcataccctt
    24781 cgtaccggac accatggagt acagagtgtc attaccgcct acaccgccga tagaatgcca
    24841 gcccaggcag atcttgctgt cagaagtcag ggcagtatat tccggctcct gataatccgt
    24901 caccggagtc tcggtctctt ccgtaatatc tcccagacgc ttgttctcca catatccgat
    24961 cacggaatcc gctgtcttca ccttgctcca ggtctccatc tgctccagaa tctccagggt
    25021 atctcccttc gctgcctgcg tgaggatcgg gctcttcaca ccgccccgca gacgcaccgc
    25081 cgtatccttt ttcagctgtg ccacctgtct ggtcccccat tcggtgtaga cctgcacatg
    25141 tctgtcataa caatcatagg aataatttgt gaacagcttc acataatccg ccgccacata
    25201 tacggtatcc ccttccacga aactgatcac atatccggca tcctgtgtgc cttctgttgt
    25261 cgtataggaa gtctcgccga aagtcgtacg gatcacttct gtcggattgg cgtacaacaa
    25321 cagtttttcc gtcatatccg cataaaacac ttcattcata tatttatgga cagttgccag
    25381 atcaaaatag catacgtcat ctctgatcag tgcctgttct tctaccattt cgtcctgtaa
    25441 aataatggca cttctgtcac tctctgacac ctgataaaat tcgttccagt ctgcctcttc
    25501 tttgctgtag gaatatttat ccaacagcac ctttcccagt gctccgcccg cgatcacaag
    25561 gatcagcagg attgctatga tcaccggtag tattcttttc ttcatatgtg ctcccatccg
    25621 caggctgcca cctgcaccat tcttgccttt tcttccgttc cgtcttctat cggcagaaca
    25681 gttttattct atcacaattc ctctatataa tacaaggaac aatagagaca gatcctgcac
    25741 tgcgggatac agccagagag gcaatcccgt tttgcatccc cgcagcgcat tctctgtttc
    25801 aaagtattac ccgggcaaga gctgacaccc tttaacgcag atccacctta tccagccgga
    25861 cctggctgat tttgccctgt tctcccaata tatagtctgc catatacggg ctttcttctc
    25921 tgacggcata cgctgccgcc tgcgacggtg agaccttttc tccgtcgaca tacaattctg
    25981 tgccgttttt ctgaagcttt cgaaaatatt tttccacctt tctcctggga aacttcttcc
    26041 cgggagtcac agcttcccgg tcctccacag acacctcccg tccgcagcat attttaccaa
    26101 aaggattcgt gaaacacttt aaaataacgc tgctttttcc gctagtacat ataagcgtaa
    26161 ctattcagaa cactattcgc aatataaatc atgtcaaatg ttcttacgaa tgcaagcatt
    26221 cgacgtacat attgacacga tttgcgtggc tctgaatagt tacttataaa tgcctctgac
    26281 agtacttgta gtaatttgtg tgaaaatgtt ggaattatgt tcgtgaaaaa tatctgttga
    26341 aatattatga ccagacacgt atatgcctgt aagacatctc catactatat tttacagatc
    26401 agcataacct atccttccca aatacattaa cagccatctg catcataaaa taaaaaaggg
    26461 gatgaactgg aacgaatcgt tccctggtaa atcaacaaga aaaaatggaa aactgtcaac
    26521 ctcctctccg taccggagta tcagaggcat ctataggtcg gattatacac tgcagcctgt
    26581 tatttgacaa gatgttttat taaatttttt taaactgtga atagcttcta ttgactaaaa
    26641 tatagggaaa gtataagcta tataccatac caatgcgaaa gcagaaagga agtgttgtcg
    26701 catgaaaata gaaaaagtaa atgaaaatca gattcgctgc accctgaccc gtgaggatct
    26761 cgccagccgc gaattaaaaa taagtgaact tgcctacggc acagaaaaag ctaaatctct
    26821 gttccgtgat atgatgcagc aggccaattt tgaatttggc ttcgaagcag aggatattcc
    26881 cctgatgatt gaagccatcc ctctgaatgc ggattgtatc gtactgatca ttaccaaagt
    26941 ggaagatccg gaagaactgg acacccgttt ttcccgcttt gcaccttccg ttacggaaga
    27001 cagtgatgat gacagtgatg agagcacggc agacgaggtt ctggatctgt tccgccgtat
    27061 ccagagcgaa gacaataccg caaacgctcc tgaagctcct tccgcgtccg ctcccgagga
    27121 gagtgacagt gtacgttccc gcctgttccg tatggaatct ctgaaacagg taatcaatgc
    27181 ggcagtaata gccggaagcc attatcatgg gctcagcact ctgtatcacg aagagggtaa
    27241 cgacgggtat tatctgatcc tgacgcaggg cgaagaagat cgcgaggtct tcgaccgtgt
    27301 ctgcaatgtc atctctgaat acggtattcc caagcgttcc acaccggcaa gccttacttt
    27361 tcttgaggaa cattgttcca cactgatcgc cgaacatgct ttacagcgtc tttctaatac
    27421 ttcaatgtaa caacagattg taacattcag gagagttccc actctgtggg gactttctta
    27481 tttttatggt tgttttatac ctgtttctcc cagcgagagt ctgtagaaaa gaagctggga
    27541 aacgcgtaac ttgaaaatat agtcttattc gcatataata aaatttaata catgaattgc
    27601 tccgttgcaa tgtagcttac gcaattccat acacattcgg aattacaaag ggtcaaaacg
    27661 ccggttacag tggaaaggat gtacacgata tgatttccga cgaagaatta taccgacaat
    27721 atttaagcgg cgatgaaaca gggctggaag aattgataag aaaatacgac aatccactga
    27781 ccttatacat caacggctat ctgcatgaca ttcacgaggc ggaggacctg atgatagaag
    27841 tattttccta cctgttcgcc aaaaggcccc atatccggga tggcggattc agggcttacc
    27901 tgtataaagc tgcgcgccat ttagctttgc gccataaaag catccggcga catcacttct
    27961 gtcttgatga tctgaccaaa gagccggaag gtgggaaact ggtggatgag gtgatccgga
    28021 caaaagaacg gaatcagatc ctgcatttat gcatgggtga actgcatccg gattacagag
    28081 aagctcttta tctgacctat tttgagggta tgagttaccg gcaggtcgct gaagttatgg
    28141 gaaaaagcgt gaagcagatc actaatataa tgtaccgggg aaaagagcgt ctgcggggat
    28201 tattggaacg ggagggaata acaaatgcgg agtcatgagg aacggatcgc agaagtccga
    28261 cgaaggattg cggaaaaaga acgacaaaaa agacggaaac agcggcggat cgttgctatt
    28321 acctgcatag cggcatgcct tgctgtgatc ttcggggctt cctttgtcat gtccgggatt
    28381 tctgggcaaa tggtgccggg gatctcctcc ggatatgaaa cggcggcaac gatcctcggc
    28441 ggaggtgctg cacccgaata tatcataata gggttgcttt catttcttct cggcgtatgc
    28501 gtgacgatcc tgtgttttcg tgtccatctg ctggacaaag aagagcaggc tgagcagagg
    28561 ggggaccgcg ccaatggaat tgatcattaa tcttttgcaa tttttcgtta ccatgctggg
    28621 cttctgtctg agcggcattc gttatctgag acatcggaca ctgtcatatt ttctgctgat
    28681 ttgcttttat ggctgtttcg cactgggctc cctgtactgg acattgtatc tgttattgtt
    28741 ttccgaaaca ccacggatct tttatgtatc ggaattcggc tgggtctcca gcgttatttt
    28801 tctctgtctc ctgcaatata ctctctcctc cccggaggaa agaagctttt cctgtcggaa
    28861 atccctgttg gctccactga tcggagttcc gttgtgcgca ttttactgta cctttggtga
    28921 cattctctcc aacctgctct ggtgcggcat gatgatcctt gtctcctatc gttccatcca
    28981 tggtcttgtc tacgcagaga cacagacggg aaaaggacgg aacctgcggt attttcacat
    29041 cggaatcctc tgctatgttg cgacagaata cgctctgtgg acctccggct gcttctggcc
    29101 ggacgactct atcttcagtc cctattgttg gtgtgatctt ctgctgacgt tctgcctttt
    29161 cgggctgctg cctgccacag gaaaggcggt gcggacatga cctatattga aaatgtattt
    29221 atctgtatgg cctcacccct gttgatcgcc gccctgtgca tgggtaggcg gcaggctaaa
    29281 ttctttctgt gctgtcttgc aggaatgggg gcatgcctgc tctctgctta catcaatacc
    29341 ttctttgccg ctctttacgc agcggatacg attgccgcca ccgcagagat cgcacctgtg
    29401 gtagaagaag tgatgaaact cctgccgctg ctcttttatc ttcttgtatt tgagcctgaa
    29461 ccggtgccga taaaaaatgc tgccatcatc gttgcacttt cttttgccac ctttgagaat
    29521 gtctgctatc tgattcaaaa cggagccgga catttttcct ttatcttttt ccggggcatt
    29581 ggaacgggtg ccatgcatgt gatctgtggt gccatcgtcg gcggtgggct ggcgtatgta
    29641 tggcagcgca cctggctgaa gattgccgga acctgcggtc tgctcggggc agccatcact
    29701 tttcatgcaa tttataatct tctgatcgcc tatggcggtg cgatacagta tatcgcatac
    29761 attctgcctg tgctgatcct gacggtggga aaactgattg tccgcctgcc ccagagtcac
    29821 aactgaatcc tgtcttttgc ggagctgctg tcggcagctc ctgttttttt gaagaaattt
    29881 cattttcagg tgagagtttt ttcgtttttt tgtacctaat tagatgaagg gcatatgtat
    29941 tttatgactt tactttccga agggaggttt tcaacgatat gtatgataca gaaaagcgag
    30001 tcgaactggt gaaaaagcgg gtgcatgaaa aatatcgccg gaagaagcgc cgcgggatct
    30061 gccggctgtc tgccctgtgc acggcattgt ctgcgttcct tgtgggaacg gtgtacactg
    30121 cagccggtca gacgcagatc accgcccggg gaatgtacgg ttctatcctg ctgcatgaag
    30181 acgccggcgg ttatgtgctg gtgggggtaa tttctttcgc cacggcggtc atgatcactg
    30241 tgctgtgcat caggctccgg gaaagagaac accgcggaca aggcacggga aaaggaaacg
    30301 catcacaaca acgggaggga aaaaataaat gaaaaaacaa agaagacgga tctatgcggc
    30361 gctgctttgc agttccatgg tactttctct ggtctccacg ccggtatcgg tagcagaaac
    30421 cgaacagatg ctgaatcccc cgacatccac agaggagctg agttccccag aaagtatatc
    30481 gggaaatgaa gcggcagctg ccctgaacag gctgtacaca gcactgccga ttgcaaacgg
    30541 tgtgaaagag gtggcgacag ccgaagaact ggcggctgca ctggcagata gcagtatttc
    30601 cgggatcaca ctgaagggtg atgttgaaat cagcagtact ctgtcagtta accgcactgt
    30661 gacgctggat ttgaacggta atgtactaaa aatgaccggc cgcggtagcg taattaaggt
    30721 agagtcgggc ggcaatctga caattgcgga cagcaacaca tccacaccac acaactttaa
    30781 cccggactat aaagattcgg cctggcacat tgacatgtgg aagctggacg atagcggctc
    30841 ggaaaccgtc tccggtggag ttattacagg cggtagcagt gattatgccc acaatgatgg
    30901 cggcggtgtg cttgttaatg ccggcggtac actgaccatg accggtggca gcatcgtcgg
    30961 atgctcggca aggagtcgag gcggcggtgt ataccttggc aaagactctg acaccggaaa
    31021 gagcggcaca ttcatcatga cgggcggaag tattatcggc tgtgcggccc agctaggcgg
    31081 cggcgtgtat gtcgcccaag agtgcacatt cgccatggct accagctcta atatccacaa
    31141 ctgcatcgca aataacgatg gtggcggtgt aataaaccac ggcacctttc aaatgcacgg
    31201 tggaacaatc agtgcatgca cgacagtagc gtttgggggc ggcggtgttt gcaacaaggg
    31261 aacctttatt atgtcagatg gtatgattaa aggctgcacc agccccgacg gtcagtatgc
    31321 ttcaggtggc ggggtgcgaa acagtaatca gttcaccatg acaggaggca caatcggtga
    31381 tccgaataat gagaatgacg caagccatgt atacaatact tccagtcagg agactactct
    31441 tactatatgc gacaatgcaa aaatctacac aaacgtaaca aacgttggca tcctgaatgc
    31501 tgacggcggc gaaatgtccg gcacaatggc gaacgatacc aacaggtacg gcaccggcac
    31561 gatcaccggc agtgcgggtg ccgccggcag cactgagttc catggcaaag tgaccaacac
    31621 cggtaccatc cggaagggca cattcaagaa tgaggtcata aatgagagca gtggtaccat
    31681 taacggcggc acattcaccg gcacgatcac gaacaatgat ggtacggttt tagacggtga
    31741 tttcagcggt gccaccaccc tgagcggtac gctcgtgatc acttttgacc caaacaacgg
    31801 ggatcagccc agcacacaga aagtgaattg gagtaagaac ggagcggcac tcaccgcacc
    31861 ggatcccatt cccaccaaag aggggcatgg tattgacggc tggtattacg ataacaacgg
    31921 tacggaaaca aaatggaact ttgacacgga tacagtcaaa tgcactatga ccctcaaggc
    31981 aaaatgggaa ctctccacct attccgtaac gctgcagaca gacggcggta cgattgccag
    32041 cggaaaagaa gtaaccggct acacttacgg caccggggca gttcttccta ccaccaatga
    32101 tattacccgc gagggatacc gctttgacgg ttggtacgca gacagcaact tttccggttc
    32161 acccgtcaca gagatcacag gtaccgatac gggaaataaa acgttctacg caaaatggac
    32221 gcaaaacacg actcccatca tctccggaaa cacgatcaat tataccgtgg agcactacaa
    32281 gacggacggc agcggctaca cgctggcgga aacagaacat tccgcaggga agacaggtga
    32341 taccgtcaca gcaacgccga aaacctatga aggctttacc tacaatccgg caatctccac
    32401 cgccagcgga acgttaaaga agatcagcag tctggaggat atcgtgactt tgaagctcta
    32461 ctatgatgtg aatgctgaca ccgaacagga aagtactgac agcggctccg aggaaaaggc
    32521 tgaccgtgag aatccctctc ctgtcgtgaa aaacaccacc ccctatatga cctataccgt
    32581 acaggcaggt gacactctct gggcaatcgc cagaaaatac aactgctcca taaccgggat
    32641 cgtggcagcc aacagtgacc ggatcaagaa cccgaaccgg attcacgccg gctggcagct
    32701 taagattccc caaagcggag cgcctatcac aggcggcact cccgatgctg tcctgcctga
    32761 gaataagaaa agcgggatat acatcgtcag acagggcgac accctctggg caatcgccag
    32821 aaaatgcggc tgctccgtgg ctgaaatcgt ttccctgaac cgggaactga tccgcgaccc
    32881 tgctctgatt tattccggct gggaactgaa agtaccacag aattagcctc ccctgaaaga
    32941 atctaccgtg ctagtcatac ttcataatat cggatcagat aatatctaag ggaaaaaggc
    33001 aaaaaaagac ggccaaaggc cgatgatctg aattccttga attcttttga tttctcaaat
    33061 ccgcgcaatc gacggacaca tttcgattgc gcggaaatct gagctatgaa attccataaa
    33121 tactttgttt cacctgtgtg ccccggtcag gcgatgtttc caaaacagaa atacaaaaat
    33181 tacatttcct cgatcacttt cataagctca tctacatcaa taccatgtac catagctgcc
    33241 tcttccaggg tctctccctg tgaagcaggg catcccaggc aatgcattcc tgcatcgagg
    33301 agtacgggag ctacttcagg attggcacgt aagatctcac caatggtcat gtcttttgtt
    33361 actccggtca tatcggtctc ctttctgccg tataaggctt ttttagtata atactttccc
    33421 cggaataagg caagattatt cttgtacatc cgcatttgtt cttccccgaa gatcagctct
    33481 gccgcacacc acattcataa aagcatttgc ttcgcaaatg gcgccgcttc gcagctcatc
    33541 ttttatttca tgttggtgtg ctttgtccat gaaaaagaga tttccgtgct ttgtgtgcaa
    33601 gcacccacgc acgtcaacct ttttttcatg tcagagctga tgtacaaaac tcggaacaat
    33661 tcctaaaaag gttgacactt acccttgtca ggtagtacac ttttatcgta aagaaacagc
    33721 ttgggatacc agtgaaaccg gtactctcag ttgataatat taattgtttt taccagaagg
    33781 aggtaaattt tcatggcaga agcatggaga agctttaaaa aagatggcca ttgggatgta
    33841 gacgtcaacg tacgtaactt catccagacc aactacactc cgtatgatgg agatgagagc
    33901 ttcctggagg gacctaccga agcaactgat aagctttggg gcagacttca ggagttacag
    33961 aaggaagagc gtgagcataa cggcgttctg gaatgcgaaa cagaggtcgt atccagtctg
    34021 accgcttatg gccctggcta tattgatgaa tctatgaagg atctggagca gatcgttggt
    34081 ctgcagacag acaagccttt aaagagagca ttcatgcctt atggtggtat caagatggct
    34141 cagcaggctg caagcaccta tggctacgaa gtaaacgcta aatatgacaa aatcttcaac
    34201 gagtaccaca agactcacaa ccaggcagta tttgatgctt ataccgatga gatgaaggtt
    34261 gctcgtcata cccatatcgt taccggtctt cccgatacct atggtcgtgg acgtatcgta
    34321 ggcgactaca gaagagttgc tctgtacggt attgatttcc tgatccagaa aaaggaagag
    34381 gataagaaga actgcggtac cggcgttatg accgaggaca tcatccgcct gagagaagag
    34441 atcgctgatc agatcaaggc tctgaagggc atgaaggaaa tggctaagat ttacggctac
    34501 gatatttccg gtcccgctac caccgctaag gaagctatcc agtggttata cttcggttac
    34561 ctggcagcta tcaagaccca gaacggtgca gctatgtccg taggacgtat ctctactttc
    34621 ctggatatct atattgagag agacttaaaa gaaggcaccc tgaccgagaa ggaagctcag
    34681 gagctgatcg accatatgac catgaagttc cgtatggtta agttcgctcg tattccttct
    34741 tacaaccagc tgttctccgg cgaccctgta tgggctactc tggaagttgg tggtatcggc
    34801 atggatggtc gtcatatggt taccaagaac gactatcgtt tcctgcatac tctggagaac
    34861 atgggaccca gccccgagcc gaacctgacc gttctgtact ccagcgctct gcctgataac
    34921 ttcaagaagt acgcagctaa gatttctgta aatacttctt ccatccagta cgagaacgat
    34981 gacgttatga agcctatctg gggtgatgac tacagcatct gctgctgcgt atccgctact
    35041 cagaccggta aggagatcca gttcttcggt gctcgtgcta acctggctaa gtgcttactg
    35101 tacgctatca acggtggtaa ggatgagaag catctggaca agaacggcaa gcatatgcag
    35161 tgtggtcccg aaatcgctcc tatcacttcc gagtaccttg atttcgacga agttatgcat
    35221 aaatatgaca tcatgcttga ctggtgtgca tctctgtatg ttaacatcct gaacctgatc
    35281 cactacatgc atgataagta ctactacgaa gcagctgaga tggctctgat cgataccgat
    35341 gtaagaagaa ccttcgcaac cggtattgca ggtctgtccc atgtagctga ctccctgtcc
    35401 gctatcaagt atgctaaggt taaggttatc cgtgatgacc agggttgtgc tgtagacttc
    35461 aagatcgaag gcgacttccc taagtatggt aacgatgatc ccagagctga tgaattcgct
    35521 gtatggctgc tgaagacctt catggagaag atcaagaaga accacaccta cagaaattcc
    35581 gagcctacca cttccgtact gaccattacc tctaacgttg tatacggtaa ggctaccggt
    35641 gctctgcctg atggtagaga agcttacaag cctttcgctc ccggtgcatc accttcctac
    35701 ggtgctgaga agagcggtct gcttgcatcc ctgaactccg tagctaagct gccttatgag
    35761 tatgctctgg atggtatctc taatacccag accatggctc cttccgctct tggtcacaac
    35821 cttgacgagc agaaagctac tctggtacgt gtaatggatg gttacttcga tcgtggtgct
    35881 catcacctca acgttaacgt attcggtacc gagaagctgt tagatgcaca ggctcatcct
    35941 gagaagcctg agtacgctaa cttcaccatc cgtgtatccg gttatgctgt taagttcatc
    36001 agcctgacca aggaacagca ggatgatgtt atcgcaagaa cctgccataa gagtctgtaa
    36061 ttcttacagg ttttgagata attgatgtat atggggccgg gcagtaatgt ccggtccctt
    36121 tttgggtaca ctattcagaa aaccaccacc cctcagctgc ctgcacggca gagaatggag
    36181 attattacaa tgatcaaagg cagaattcac tcattagaat ccttcggcgc agtagacgga
    36241 cccggaatcc gctatcttat tttcctcaag ggctgcaata tgcgttgtca atattgccac
    36301 aacgtagata cctggaatcc cgacactgac aatctgatga cagcagacga gcttctggat
    36361 aaagcagagc gtttccgcag ctactggggc aaagaaggcg gcatcaccgt cagcggcggc
    36421 gaagccttat tacagatcga ttttctgatc gatcttttcc gcaaggccca cgagagaggc
    36481 atcaatacta atcttgatac ttccggacag ccttttacca gagaagagcc tttcttctcc
    36541 aaattcaatg aattactgaa atatactgat ctcgtcatgt tggacatcaa acatattgat
    36601 gatgaagaac acaagaagct aaccggccat accaacaaga atattctaga ctgcgcaaga
    36661 tacctttccg agcagggtat tcccatgtgg atccgtcatg tactggtgcc cggaattact
    36721 gacaatgacg aatacctgaa aaagacaaga gaatttattg atactctgga taccgtcatg
    36781 aaagtagaag ttcttcccta ccatactctg ggtgagtaca agtggaaaga actggggatt
    36841 ccttacaagc ttgagggtgt agaaccgccc accgaagaga ggatccagaa cgcgaagaag
    36901 attctggagt tcaataaata taaataagca actgtaactc ttgataaaaa atactaaata
    36961 aggcttgtat tcaaagccca tttatattac aatacaagta gttatattga taaatttgta
    37021 tcaaacaaga gatttgatac acgcaaaata ggaggaaaag attatggcat ttgttattgg
    37081 tgatgcttgt gtagcatgtg gagcttgtga ggcacagtgc cccgttggcg ctatcagcat
    37141 gggcgacgga aagtttgaaa tcgatgcaga taagtgcatc agctgcggtt cctgcgcagg
    37201 tcagtgccct gttggtgcta tcgcagagga ttaattcctg attcagaacg taccccgggg
    37261 aaggcatttc agaaaatgaa atgccttttc tttttttctg ccttaatttc cctgccggta
    37321 ttttctttgt ctgcgatctc tgtggactct ttttccactt tcggccttct ctcctctttc
    37381 tgctgtttcc gcagttgtcc cctggtcttt ttccgcaaca gctcgtccat cttctgatag
    37441 tactcctcgc tgcgctgttc cagtctgcgg tctctctcct cttcccgttc ttctctgctg
    37501 cggaactgat aatccagctc cttgaccatg ctttccttga gttcccggca aaggtcctgg
    37561 ttattctccc gtactgcctc acggatcaac tgttgcagga tccactggag ccttttagcc
    37621 ttctcttccc tgctttcact ctgcagtcct tccttctccg gcaccgtaca tatcctctcc
    37681 tgcggctgta ccggcatctc tttatccacc ggtttatcaa cgatctcgat ggccatgtgt
    37741 ggtgtccgga cttctgccgg ctctttccgg gcttctacag gctctttttc ccgttcctcc
    37801 ggcagtacgt ccagccgtcc gtcctttaat atcattttaa ttgctttcaa ctgtaatccc
    37861 ctttctttca tgctcttgat ctctttgaac cgttccacgt cttccttcgt ataatagcgg
    37921 tgccccagct cgtttctttt gatgggcaga tgtaattcct cttcccaata gcgaagtaca
    37981 tggctctcca cctttacctg ttttgcagca tccgaaatca ataaatactc tcccatattc
    38041 tcttcctttc cacatagcca tatataatga tgtcggggtc aaaagccccc gactttgatg
    38101 cctacggatt gttccgtgct
//
LOCUS       DAWDHD010000007        34593 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_105_length_34593_cov_12.204934, whole genome shotgun sequence.
ACCESSION   DAWDHD010000007 DAWDHD010000000
VERSION     DAWDHD010000007.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 34593)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 34593)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..34593
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105_length_34593_cov_12.204934"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    31097..31196
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         8664 a         9256 c         6965 g         9608 t
ORIGIN      
        1 cggcgtgacc tattgataga agatcctttt gcctgcaccg cgtttatgaa ttgcaaaaac
       61 acggagatga tacggagatg attatgaaac cacccttagc taccaaaaaa ctgacgacgc
      121 tttccctgct gtctgccatt gcgctggcgc tgtatgccgt ggaaagtgct ctgcccccca
      181 ttgttccgat ccccggcgtt aagctggggc ttgccaatat aataacgctt gttgttctat
      241 ggaaatattc cgcaaaagat gcttttttcg tgcttttggt gcggattctt ttagcgaccc
      301 tgttcttcgg tcaggccatc agtctcctgt acagtctctc cggcggtctc ctgtgccttc
      361 tggcaatggt atttgtgcaa cggctgttgc atggacatta tctgttcctt gcaagtatga
      421 ccggagctgt ttttcataat ctggggcaga tcggtgttgc gtttttactg acttctgttc
      481 ccgctgtgct ggtctatctg ccgtttttac tgttaagcgg tctggtgacc ggtctgttca
      541 tcggtctctg tgcccgtttt acgcttcgtt tccttccgta agatccacag gaagctccga
      601 taacatctgt gttacggtct tgtgcgtaat gggatgacgg aaattcggtg ccagctcact
      661 caaaggcttc agtacaaaat aacgattctc catatctata tgcggaatca acagatcctc
      721 ggaagcatac accagatcat catagaagat aatatccaga tccagagtcc gtggacccca
      781 gtgaagctta cgttctcttc cttccatctg ctcaatacgg tgcagctcct gtaataattc
      841 ttccggtgca agcagcgtcc ggatctcaaa cattccattt acgaaattat cctgctctac
      901 gacgccataa ggtagtgtct ccaacagttc cgacaccttt tccactctga tgtctttgtt
      961 cttctgaagc tgtcccaaag cctgctcgat atgctctctg ctgtctccca tattggaacc
     1021 cacggcaata taggcttcat gccattccct gtgaatcgcc acagatacac tgccaaaggg
     1081 taatgggatc ggagcctcgg gcttgcggat ctccaaatcc agtccctgta ccagcggaaa
     1141 atgctgtaat acttcgtaag cagtccgctc cgccaccgtt tcgatcagtt gaaaagtatg
     1201 ctgctgcatg aactccgtga taaactgaca gacctctccg taattggtgg agagacttaa
     1261 gtcatccgcc agtcctgccg gacgggtatt cgtatataac gtggcattca cataaaaatg
     1321 ctgtcctttt tcattttctt ccggatatac gccgtgatgc gcgtatactt ccaattcttc
     1381 gatacggatc tggtcctcat aagtgccttt tttcatttgc cgactccgcc tttcccaacg
     1441 cttcttcgca aaatatcatt tgctgctgtt taaaatggct tcacacattt taatggtgcg
     1501 gacattttcc ttcacatcat gcacacggac gaacatgcat cccttctgca caccgaacat
     1561 ggtggtcaca agcgtcccct ctactctctc tgtcaccggc agatccagtg tcagtccgat
     1621 cacggattta cggctgcatc ctaaaagcaa cggataaccg aacatattca gttcttccag
     1681 acagttgatg atctccagat tgttctcata ggtcttgccg aagcccaccc cggggtctaa
     1741 gatgatcttc tcatccgcga tgcctgccgc ttccgccaaa tggatcgtgt ccgccagatc
     1801 cgctaccaca tcctgcatga aatcccgata atcggcttcc ttacggttat gcatcagaca
     1861 gcagggcaga ccgctcttcg caataacctc tgccatacgg ttatcatatt tcagtcccca
     1921 gatatcattg atgagatcag cccccgcacg gattcccgcc tctgccacac cgcttttgta
     1981 ggtgtccagg gaaatgggaa tgtcaaaatt tgccttcacc acctcgatca tgggtacgac
     2041 tctggcaatc tcctcctcgt cagatagcag ggtataaccc gatcgggtgg actctccgcc
     2101 gatatccaca atatccatcc cctcagcaat catttcctcc acatgcttca gtgcccggtc
     2161 tctgtcattc cactttcctc cgtcggaaaa agaatccggg gtcacattca ggatccccat
     2221 tacataagta tgtcctttgg tctgaaactc tctgttgcca atcttcattt gtcttctccc
     2281 cacatttgtt aatattattt atgatgttcc cgacaccaat caatacttaa ttgtcaggtt
     2341 cttcttgccg tctttccagt tgcaatcctt ctccacatcg caggcgaagc aggcacggct
     2401 tgccttgtcc gcccggtaca ggatgccatt tagattcttc tccttcgccg cctcaggatc
     2461 ccggtgtctg gagattgccg ttacaataac acccgtttct tcatcgttga atccacacgc
     2521 cttcaggatc tccggtgcaa tccgcgcact ggcctgctca tgcgggatac caatctcata
     2581 ctgttcatgt ttcccacaat cgtggagcag tgccgccgca tagatccatt cccggtcgat
     2641 cttaagccct tcctccagcg ctatgatcgt accgatcctg gctacatcca gaaaatgcac
     2701 cataccatga cggcagaagc gtctgtctgc ttctgccgca ttgtttcgtc ctaaatgata
     2761 taaaaacaaa tcgtgattta atattttatc aattctatcc atcaaagtct caccattccg
     2821 tcccggtctc ttaacagttt tcatccgtat ccgcaatgtc taccggttca ccagctgtaa
     2881 aaatctgtcc tgcaattcct tattctccgc aaagactccc cttgtcacca tggtaacggt
     2941 ctggctgccg ggctttttga tcccgcgcat ggtcatgcac atatgctctg cttccaacat
     3001 taccataact ccctggggtg ccagatattt catgatatca tcggcgatct gcgccgtcat
     3061 ccgctcctgg atctgtaatc ttctggcata tacctccaca gtcctggcaa gcttactcag
     3121 acccacaacc ttgccatctg ggatatacgc aacatgagct ttgccataaa aaggcatcag
     3181 atgatgctcg cacatagaat agaaggtaat atccttctcc agtaccatct cattattatc
     3241 tacagagaag accttcgaca gatgctctcc cgcatcctga tccataccgc cgcagatctc
     3301 ttcatacatc ctgccgatcc gctccggtgt ctccttcagt ccttctctat cggtatcttc
     3361 cccgattcct tccagtaaaa gctttattgc ttcctgtact ttcttctgat ctaccatttt
     3421 caccgcctcg ctttccgttt attccctgct gtctgtctct tctccatgag cttcaggatt
     3481 cttcccagat aagagagaga gccgaagcat aatatcacat catcccgtcc tgccagcagg
     3541 tgactaagct ccactgcctc ttccaggctg tccaccgccg tcacattggg gtgtaccttt
     3601 gccacctccg ttgccagatc ataagcatgc atggctctgg gattgtccgg aggtgtcacc
     3661 gtgagaatct gatccgcata cttctccgtc agcgcgatca cgcggtccac ttccttgtcc
     3721 tttaacattc ccattatata gataattctt ttgtttgtaa aatagaattc tatggaatcc
     3781 gcaagttttg cggctgcatc ctcgttatgc gcgccgtcca ctataaataa cgggtgttct
     3841 tccagtattt gcagccttcc gttccagaca gtctccttca ggcctttccg aatggcagtc
     3901 tctttgatct cataaccgca tttccccaga gcctgcaccg cttctaccgc cagcacagca
     3961 tttgctacct ggaatttccc cgcaagggtg atctccagct ttttataatt tccataatcg
     4021 aaggtctgct ttttcagtcc gtatttcaca tgctttgcat ttgccacatc tgcgatggtc
     4081 agcatgcagc caagctctgc cgccgtctgc tccacaacct tttgtgccgc ttccttttgc
     4141 cgcatggaga ccacctgaca tcccggctta cagattccgg ccttatgtgc tgcaatctcc
     4201 tccagactgc tacccaagaa cttcatgtga tccataccga tggatgccag taccgccacc
     4261 tgcgtggtgg tgatcagatt tgtagcatcc tccctgccgc ccataccggt ctccagcacg
     4321 acgatatcgc actgtttctc ccggaaatac caaaaagcca gcgccgtctc gatctcaaaa
     4381 ggcgtgggat gatggaaccc gtcctgcacc attgcctcgc aatgctcccg gatgatttcc
     4441 agaccatcgc acagatcctt atgggtgatc ttctgctttc cgatctggat tttttcacaa
     4501 tattcaataa taacaggtga tatatatttt cccacacggt atccggcagc ctgtaagata
     4561 ttggaaatat aggcaagtgt ggaacctttt ccgttggttc ccgcaatatg tacgaatttc
     4621 aggtcgttct gcggatctcc cagccttctg cacagctctc tgatgctgtc cagccccgga
     4681 acaatgccgt aggttcccac tttttccata tattccattg cttcctgata gttcatagca
     4741 atctctcccg tatcatacac tgaaaatttc atatactatg tccatgaaaa tgacaaaatc
     4801 catcgcaaac tcttttaaaa ccttcggcag gagctttctc ctgtgcgggc tgtgcggctg
     4861 gtgcatggag atcctgttca cagcgctgca ctcccttcgg cgcagggatc tgcgtcttac
     4921 cggtaatact tccctgtgga tgttccctat ctacggctcc gcctgcctgc ttgcaccctt
     4981 caaacggctt tttgagcacc tgaatatcag tcgtcccggg cgcgggctgt tctatatggc
     5041 tatgatcttc accgtggaat atctcagtgg gcgcctattg tggaagcata atctctgccc
     5101 ctgggattac ggtaaaagcc gtttccacat ccaccggatc atccggctgg actatgctcc
     5161 ctggtggttc ctggcaggac tgttttttga aaagatcctg ccctgagccg ttttactgca
     5221 ttactgcatt gcttctgcca gcatccgttc ctcatgaccc tttttgtccc tcaccatctt
     5281 cttcatgagg aaatgataag cgatccagag gaaaatacct gcggtcagcc aggctgaggc
     5341 attggcaaag cacacaccca cataactcat cagatgcgct gccgcaaaag ccacgatacc
     5401 acggctcaac aattccacca caccgcccat catgggcatg aagccgaagc cacagccctg
     5461 cagcgcattt cggaagataa atatcattcc cagcggaatg aagaacatgc cgcagatcgt
     5521 aatatacgtt ctggcatatc cacataccat ctcaacctgt tcggagatga acagcggtac
     5581 aatataagga agtgctatat atactagtcc gtagacaagc accgcataga caccggacat
     5641 gatattggca gctttcacgc ccttacggat ccggttcaga tcattgatgc cgcggttctg
     5701 cgcacagtaa gttgccatag tcacacccat tgcagcaaag ggctgtgtca ccagctgttc
     5761 caccttgcag gacactgaat aagatgccac cacggtagaa cccagcagat tcagtgctgc
     5821 ctgtaccaga atagtaccga tagctgtaat ggaaaactgc agtgccatgg gaattcccac
     5881 ggtcagctga tttttcacac actgactttc cagtctgcaa tgctccggac ggatatgcag
     5941 tgtaggtact ctcttaataa tataaatcag acacagtaca ccggataatc cctgagaaat
     6001 gatggtagca taagctgcac cgcccactcc catatgaccg taaacgatca gtacataatc
     6061 caagacaata ttcgtcaccg aagcgatcac cagcaatact aacggcacca cactgttgcc
     6121 gatggcacgc aggatactgg acaacaggtt atacaataca ttgcaggcaa ttcccgcaca
     6181 gatgatcata atgtattcct tcgtcatatc gaaaatatct tctggagtat tcatcacccg
     6241 aagcagactg tccatgccgg ccatactgac tgctgtcatc acgatcgtca cgatcaccga
     6301 caggatggca gcacttccga tacttttgcg cataccttcc cggtcccctg ctccgaagcg
     6361 ctgtgccgtc agcacggtaa atccggtggt cagtccctgt gtaaatccca ggatcaggaa
     6421 gcttaccgac ccggtggctc ctactgccgc caatgcatcc acacctatga accgacccac
     6481 gataatggta tccaccatgc tgtatagctg ttggaaaata ttacctatga taataggaat
     6541 gataaatttt gtaatcagct tagaagggct gcccttcgtc atatcaagtt ccataaatct
     6601 cttctcccga aaaaacagga ccccggaagg ccccatatct tatactacat ttactatatg
     6661 atgccggggc aaaagccctg gctttgatgc ctgtaaattg tttcgtattt ttgctcatac
     6721 gattactgta agtcgtcaat atcctcaacc tctccggaac cgatatccag catgttcacg
     6781 gttctgcgct tcaatctggc ttcttccgac tccttcaaaa taaactcctt gatcgctttg
     6841 gagttgcgga tatgctcgat caacagtttg ggatgcgtag tgtcaccggt ataacagaca
     6901 atggtaccaa gtcctgccat tctctcccag atacttgccg tatgctccac atcctgtacc
     6961 ttgtacatat agcagtcatt ctctaccttg gtgaacacac ccgtatccac agtgatcata
     7021 tcctctttga tcacatactt ggtaaaagta aaaggtaatc ctaaaaataa ccagcgcttc
     7081 ttttccacaa attctataga catagtgccc tccttatatt gcttgatccg gtgtcccaca
     7141 gctgtctata acgtacagcg gaaccggctt ttacacgatc tgcttcatcg gtataattct
     7201 ctttgcctca ttatatgata tccattccag gaatgcaaaa cctttctgtc atagccatac
     7261 aaattttcta tattttttca ctttttcctg cttgattctc cccttcagaa atgctattat
     7321 attacaaatg caaaaataca ggaggataaa cacatgaccg caaaagaatg tattacaggc
     7381 cgcagaagta tccgtcagta tacggatcag ccggtatctc atgagcttct ggctcagatc
     7441 gtagagacag cttcttatgc tcccagctgg aagcatacac aaatcgttcg ttatatcgct
     7501 gtggaaggcg aaaagaaagc aaaactggca gcatgtactt cctccttccc gggtaatggc
     7561 aagatcatgg agaacgctcc catggtcgtt gcagtgaccg tgatcaaggg cagaagcggt
     7621 ttcgagagag acggctcctt cactaccgcc agaggcgatg catggcagat gttcgatgcc
     7681 ggtgttgcca gcgaagcatt ttgcctggct gcttacgatc agggtcttgg caccgtgatc
     7741 ataggtattt tcgatcagca ggaagctgct gatctccttg aaattcctga aaatcaggat
     7801 ctgatcgcac tgattcccat cggttatccc gcagaagcac ccgttgctcc caaacggaaa
     7861 cccgtaacgg atctgttatc ttatcaacag taattttaaa gtcgaagagt tttcctcttc
     7921 gacttttttc tggtcagggt atgaactgtc cgcttcctgc ggaccctgtt tctacccgca
     7981 tcataagaaa agcgctgtat tcttatgatg tcgcatacac aagtgagtac ggattctcct
     8041 attccgtatg gtcactgtgt cgaaaatagc agcgcagaaa tgaggaaatg aaaaaatgag
     8101 acacttgatg aacccacttg acttctctgt tgaggaactg gacaagctgt tcgctctggc
     8161 agacgacatt gagaaggatc ccgccaaata cgcccacaaa tgcgacggca agatcctcgc
     8221 cacctgcttc tacgagccca gcacccgcac acgcttaagc tttgaatccg ccatgacacg
     8281 cctgggcggc agagtcatcg gcttctccga tgcggcttcc tcttctgctt ctaagggaga
     8341 aagtgtatcc gataccatcc gcatcatctc ctgctatgcg gacatctgcg ctatgcgcca
     8401 tcccaaggaa ggcgctccca tggtagctgc agagaaatcc ctgatccccg tgatcaatgc
     8461 cggtgacggc ggccatcagc atcccaccca gaccctgact gacctgcaga ccatccgcag
     8521 cctccacggc gatctgaata attttaccat cggtctgtgc ggtgacctga aattcggtcg
     8581 taccgtacac tccctgatca acgcactggt gcgctacgaa ggtatcaaat tcatcttcat
     8641 ctctcccgaa gagctgaaga tccctgacta tgtcaccgac atgctccgcg agaaaggcat
     8701 tcctttccag gaagttatcc gtctcgaaga tgtgatgccg gaactggatc tcttatacat
     8761 gacccgtgtg cagaaggaac gtttcttcaa cgaagaggat tacgtccgcc tgaaagattt
     8821 ctatgtactg accccggaaa aaatggaact ggcgaagccc gacatgctgg tgctgcatcc
     8881 ccttcccaga gtcaatgaga tctccgtgga gatcgatgac gatcccagag ccgcctattt
     8941 caaacaggca cagttcggtg tctatgtccg tatggctctg atcctgactt tgctgggtct
     9001 ggcataattt tccggcatct gaccgcaaaa ttatcttatg aattcgtata ttaaagctaa
     9061 catacgcagc actctgtgcc tgcagaatgg agattactat gttaaatgta ggaaaaatcg
     9121 aagagggctt cgtcctcgat catatcaaag ccggaaaaag tctctcaatc tatgaacacc
     9181 ttcagctgga taagttagac tgcaccgttg ctatcatcaa aaacgcccgc agcggaaaaa
     9241 tgggcaaaaa agatattctg aaagtagaat gtgatatcga tatgctggat ctggatgtgc
     9301 tggcattcat cgaccacaac atcaccgtca acgtcatcaa aaatggtgag atcgtagaga
     9361 agaaagaact ggtacttccc aaacagatca aaaacgtcat caaatgccgc aatcccagat
     9421 gcatcacttc catcgagcag ggactgcccc acgtatttgt cctctccgat gagaaaaaag
     9481 agatctaccg ctgcaaatac tgcgaagaga aatacagcga aagctaaatc ttatacaaca
     9541 aatgccctat cagcagccgc tggtggggca tatttacaat acttattccg catcatcctc
     9601 tgttttcttt tcactatctt cctgcttctg catatacatt atttctaacc ttacctgaat
     9661 tggcagtaat agcagaaaac caattaatcc atataaaatt ccatttaatg caattgaaat
     9721 ggaaatactt aatcctgtaa aaagaagtgt tccttccccc ataggttgaa ttgccataaa
     9781 gctgatacag ctttcgagga atcccattcc caaaaatgtg attttcgcaa gctttatcgc
     9841 tgattttgcc tgaaacaatt gttttattga tactgatttc ttttttccaa aaataatccg
     9901 aaaggaatcg aaaaagggta gtcccaaatt tgtccccagc aaaaataaag cagtaactaa
     9961 aaacatactc aataatgtag gcatatccac aaaaagcata ttcatactga ttcccatatt
    10021 ggtaataaca gaatataaaa caacgaataa aaaaattatc acagttatcc caaaagccaa
    10081 taatgaactt cggcttttct ttttgctatt ctctgtttcc tgtaaaagat ttatcatatt
    10141 gacttcagct ttttcagtgt aaccgttttc caacaaatgt tcacctgaca ggaactcatt
    10201 aatactaatt tgcagaatgt ggcataacgg aacaatcaat gccagttccg gcattccttt
    10261 gccacattcc catttggata ttgttttgtt actgacacca atcgcatccg ccaattgttg
    10321 ttgcgtcatt ccctgctctt ttcttaatgt agaaatgaat accccaatct tttcctggtc
    10381 cattgctgtt cctcccgtaa aactataaaa atacgatttt catgactgta ttttacaatt
    10441 ttatcgctat gaggacaact tttccttcgt agaccccagt ctatctatcg tagattttca
    10501 ttgtacataa catctttctg cgtatttcaa caatcttatc tacacattat ctgcgacata
    10561 aaatgtgggc gccggcatat tttcccactc tgttaacgta atatttcctt ttgtaatttc
    10621 cgctgcttcc aataatccct gcattgtttc atcaaaaaag ttactcataa gttttcctcc
    10681 gtagtctgtt gcgttttctc tttattttac tcagtctcat tatgtatcaa atctcgttca
    10741 attccttttc cataattgtt ttaattgctc taatatctgt aaaagtcaac cttacgccgc
    10801 gattatacat agccaatact tcattcaaat tttctactag caatttatta agctttttat
    10861 gtattgtcat cggctgatcc tgcactgtat gaatatgatc aaaatatttt tcaatatgca
    10921 aacaacataa aacaaacggt aaatcataat taataacttt gttctttaat acaaacaatt
    10981 gctcctgtcg cattattctc ttctacccac ttcttaatca tatcagtgga aaacctgaca
    11041 tccccaaaaa tccgactatc tggtttcaat attatctctg tacctgacga atgttccatt
    11101 tcacaagaat tcacgtcatg ttgggcaatt ccccttgcat agtcctgact atagcagtta
    11161 ctatctctat acacattaat ccgcaaattt tcgcataatg aatttatcac ctgcatgcca
    11221 ccttgggcaa aatcacccat ctgtgcatat tctatactcg atataggatg acctgaaagt
    11281 attttatcca gtacctgctg atttgtcttt tcattttgtg aaagaggaat tcctcttcca
    11341 ttatcagtta tctttactct tccatcctca tacagtgtaa tattaatttc cgtgcaaaat
    11401 cctgcatcgg cctcttcaag cgacgtttta agtacttcat aaacaatctg atgcatatta
    11461 tctattaagc tttccttatc tgctcccata cgcattctcc ttccttctat gtttggcaca
    11521 caatattctg gcaccttata cgaagtaaga tattttttca ccttacttgg cgtggaacca
    11581 aacaattgtg taaatgaacg tgtaaatcct tcgtgagaat caaatgcata cataagtgat
    11641 acatcaagaa ctttcctacc ctccaacaaa gaaccaagcg catattgtag ctttctgatt
    11701 ctaatgtatc cggttattgg catacccata atttctgaaa ataatttact gaaatagaat
    11761 ggactataac cagccatttc tgccaattcc agcaaactga ttttttcata aatatgttca
    11821 tctatataag ccaaaatctc ctttaaaatt tctgtactca ttccctttct cctcacatac
    11881 agtatcatgg atgaagccca ccatacattc tacaagcagg atgcttcata tccaacctgt
    11941 atatatagca tacacgctgg gagtagatat ttcttgattc catatatgaa ataatatatt
    12001 ttctatctca aacttttcat aacccttttt gcccaagcat cagaatcatt acgctctctt
    12061 ttcatgagct ttattcaaag gcttcttatc ttctttgtcc ctgcacttcc ataatgccat
    12121 tggatgcttt gctacatttc taaactcatc actatccccg aatacccagt caggataata
    12181 atttccttct gagaaagcct tccagaacag cttttcctcc tttgtgggca ccagaacaga
    12241 tgtaagatag tcttttactt ccttttgtac aaattccagg tcaaatcgtt ctcctttacg
    12301 aagcactgaa ttcaaatcac gcttaatctg ccggaatgac acgttcccga tattatcaag
    12361 cgcaaatttt tccggtggct gcgctgaccc gattgcacta tagaagacga cacatttacg
    12421 aagcatcgct tcctcacttt catcaaatag cccaaatttt accatgttat gcatatcata
    12481 taaatctcgt ggagctgttc ttgtaagtaa tgctactgtt ttagaagcaa atatctccaa
    12541 tggtgctacg gaaaaaacgg taagcttttc ctcattccat ggcagtctta cttctctccg
    12601 tgccacagga agcacatgac agcgcagcat atagttaatc tcaatcttaa gattatcctt
    12661 tgtccctccg caatttatat attcatacac aaaggaatcc agtgcatgat aactctttga
    12721 tttcggactc agtacatatc cgtttgccat catatacttg ctgatttttt ctgtgataat
    12781 ccccccctgt ctgcaagcat tttttccctt tcaatacttc tgcaataatc caaatcaata
    12841 tccaccgaaa gtcttggcaa atgcgtgtct ctcggaaaac ccagttcttt tgccattctg
    12901 cccagagtta atctatccca ttgcatcata agcatccacc cccttatcta tcagtccttt
    12961 caacgaaacc ggtgcataaa gcttccatct tttatgaaat acattatcct gcgattcctt
    13021 catcaggtag cgctttgcat cagaggaacc attctcacac gtttcaaaaa actgggctga
    13081 aaattggaat tcttcctgca tttcttcaaa aaaatatccg catttctgat acagaaaacc
    13141 attgttgttt cttgccagac attccaacac tttctgttca ttcagtcccg gcaccagcat
    13201 catgcaccgt attacttcct caattcctgc tatcttttca aaatcacgta tactgtctac
    13261 aacagtctgt tccatggatg tcactcgtat ttttccctgc tgtactactt ccatatctgg
    13321 tttacgctct atgtggcggt acaacactcc gtcatactca aaatcgtgaa atcgtttatc
    13381 cgttgcaaca taacactcat aaaacacctg acttccatac ccaaatacct caaatgcact
    13441 gtgatgtgtg atacaagcat ctgggaacag attggaacct atctgataac gggataacac
    13501 aggctgtttc gtttccatgc tgattaccac ataaaaatct cttttcactt tttctatcag
    13561 cccttttttc tggtactcat aaattacctg attcgctgcg gttgctgttc ctacaatttc
    13621 tatcagctgt tctctcgaaa aagaaccctt tgcaagtagc ttttcataat gcttcatcat
    13681 gtatcatctc ctaatgtttt aaattcaacg caatgtatta tttattctta atactctata
    13741 cttttttaat aatccatttt aattttatta catagtatta gaaatatcaa atacattgtg
    13801 cactttttga aattacattt tcctcatcat ctcaaaacac agcagacaaa agacaaacat
    13861 tacttaaaat gttacttctt tcaacgtttt tgcagaccat atatacacgt aactttgatt
    13921 atttggtatt tctgcacgaa tctctgcaaa atcgatagac gacaccagcc tacgctgaca
    13981 atagattttt ataagaaatg tttatggaag aagtatatcc tatatcttct gttttggcaa
    14041 gaaaaaaact cacctgtatg ttaccacaca ggtgagttcg atttattttt acactattct
    14101 gttcacaaaa gcttatctct gtacacgtcc ggaagcatcg ccgccagcca gggtctcaac
    14161 ttcttttctt gataccaggt tgaagtcgcc ggggatggtg tgcttcagag cggatgcagc
    14221 tacgccgtac tcaagagcat ccttgaagtt cttgccatcc agcataccgc agatcacgcc
    14281 gcctgcgaag gagtcgccgc cgcctacacg gtcaacgatg ggatggatgc tgtactcttt
    14341 ggagtgatag aactccttgg tatctctgga gtagatgcaa gcagaccagc cgttatcgga
    14401 agcggaatgg cttacacgca gagaggatac gcagtactcg aagttgaact tgtcaaccat
    14461 ctgctcgaag atggacttgt aaccagccag ctccagctca ccgctggtaa catcggtatc
    14521 gccgggcttg aagccaagta ccagctcagc atcttcttcg ttaccgatac atacatcaac
    14581 gtacttcatc aggttggtca taaccttctg agccttctcg gagctccaca gcttcttacg
    14641 gaagttcagg tctacggata cctttacgcc gtgcttctta gcagcgatca gagccttctc
    14701 ggtcagctct gcagcagcgt cggatacagc gggagtgata cctgtgaagt ggaaccagtc
    14761 agcaccttcg aagatctcat caaagttgaa atcagcctcg gttgcggtag agatagaaga
    14821 atgtgctcta tcgtatacaa ccttggaagg tctcatggaa gaaccgctct ccaggtagta
    14881 gatacccagt ctctcaccgc atcttgcgat gtgcttggtc tctacgttgt actttctcag
    14941 agctgcgact gcgcactcac cgatctcgtt gtcaggaact gcggtaacga actcagcgtc
    15001 atgaccgtag ttagccagag atactgctac gttagcttca ccgccgccgt agtttacatc
    15061 gaactcgtct gcctggataa atttctcgtt gttgggggtg gacagtctca gcatgatctc
    15121 acccatggta attacttttg ccattttggt atattccttt ctcaatctat aacattattg
    15181 attattactt tgcgctatac tatcgatcgt atagaatagg cttctttcaa tacacaggtt
    15241 gcagtgactg cgtgttttag ttgcagactc taaacgaccg tcggtactta gatgccgtac
    15301 tttggcatct tatgtaccgc tacgggagag ccgaagcgga agcgcgtctg caactgaaat
    15361 atgcagttgc tgcaaccgta cggcttaaaa tttgcttatg atttatttct gtaccagatg
    15421 aatagcgaag ccgccgatct cctctttcag gtagatagcc ttcaggttac ccttggcatc
    15481 tctcttagcg gactcctcgt tgaactcaac gcccagaacg gtgctcaggt agttgacagc
    15541 tctctcgatg tagttggtct taacagcgat gtgaccgttc ttgcccaggt aaggagtctt
    15601 cataacttcc agagcagagc ctgcgaatac ggagctgttg ccaaccttag cgggcatacc
    15661 aaagataaat gcgaaacggt ttgcagcctt aacagcctct tcctcgttct ctgcattgac
    15721 accaacatga gccagttcga agcccagcat ggtctgtaca gcttctctgg tcagctgctc
    15781 gatcttgtcc cactcaccgg cgttgatcag gtcgccggga accatccagg aaccaccgca
    15841 tgcgatgatc ttggggaagt caaggtaaga agtcaggttc ttagcgttta caccgccggt
    15901 aggcatgaac ttcatattta cataaggagc tgccatagcc ttgatcttag ccagaccgcc
    15961 agactgctca gcggggaaga acttaactac atccaggcca agctcgatag cctgctcgat
    16021 gtcgctgggg ctggaagtac cgggagtgat aggaatgttc ttctctacac aatacttaac
    16081 ggttctggga tttaagccgg ggcttacgat gaacttagcg cctgctgcta ctgctgcgtc
    16141 aacctgctct gcgttcagaa cggtacctgc accaacacac atgctgggga agttgtcggt
    16201 catgatcttg atagcttctg ctgctgcgct ggtacggaag gtaacctctg cacagggaag
    16261 tccgcctgcg caaagtgcct ttgccagagg aagtgcatct gctgcgttgt caatttttac
    16321 aaccggtacg ataccgattt tgctgatttg ctctaatact gcgttcataa ataacacctt
    16381 tctgtcttat agactaatag ttatgtgaaa aatcttcgca ggagatcctg tcgaaaatat
    16441 ttctgcaaaa tgaaaccaat acttttatgc cagtacttcc ttcactgcct cagcgatctt
    16501 gtcgcacttg cagtttgcaa agaaatactc cttaaacctg gcaccggata aagcaccctt
    16561 taccagttca cgatccagat tcttcaggat ggtaaccata tcggtatgag ttacttcttt
    16621 tacctgatcc aggatcttct tgttgcgctg ctcgggaaca actctctcag ggggatatcc
    16681 accgccgccg ggagcacaga acagcttttc aaaagtatac tgtaagttca gttcaccgcc
    16741 ccagccgaag ttctcagcaa aaggaagtgc tacacagtta ccgtcgttta cctgagagaa
    16801 caggtaagca tccagagggg actcaatgtg tccacacaga accttgggga aggaattgca
    16861 tgcaagcatg gcaccttcgc cggttccgca tccggtgata acgaaatccg ctgccccgga
    16921 gttcaaaaga actgctgcca gaataccgtt ctgtacgtag gtcagggaat tgggatcctc
    16981 tgcgccgcac ataccgtagt tgaatacttc atgacccatg ggctctacta cttttttaag
    17041 agtagcgcag atcatttcat tcttagcagc ctgactgttt tcattaatca aagctatctt
    17101 catgagatac tccttttcca aacgccacaa cagtcagccg ctgaaattct tctgcgactg
    17161 acctgatgtg aagtgtttat tctatttttg atatcctgcg taaatgcaca agctccatag
    17221 ctactcactc gcaaattcgt gcttcgcact ctgcatccgg ctcgtaaact caccggatat
    17281 ttactcgtga gctgtgcaag gaaaacaaat gtccgctacg cggccgcttg ttttcctttt
    17341 gcacaagttc tactggggct gcttgccgat gtaagcaagg ataccgccat ctacatacag
    17401 gatgtgtccg ttgactgcat tggatgcctc agaagcaagg aatacagcgg ggccggtcag
    17461 ctcttcgggc tttaaccatc tgttagcagg ggtcttagcg cagatgaact ggtcgaaagg
    17521 atgtctgctg ccatcgggct ggatctcacg aagaggagcc gtctgaggag tctcgatata
    17581 accgggtcca aggccgttac actgaatgtt gtactggccg tactcggaac agatgtttct
    17641 ggtcagcatc tttaagccgc ccttagctgc tgcatatgca gatacagtct cacgacccag
    17701 ctcggacatc atggaacaga tgttgatgat cttaccatga cccttcttga tcattgcagg
    17761 gatcactgct ttcgatacga tgaaaggtgc attcaggtct acgtcgatta cctgacggaa
    17821 ctgatctgcg gtcatctcgg tcatagggat tctcttgatg atgcctgcgt tgttaaccag
    17881 gatatcgatg gtgcctactt ccttctcgat ctgagctacc atagcattaa ctgcttcttc
    17941 gttggtaacg tcgcatacat agccgtgagc cttgatacct ttctcagcgt atgctgcgat
    18001 tcccttgtct accagctcct gcttgatatc gttgaagcag atggtagcac cgcactcagc
    18061 gtatgcggaa gcgatagcga aaccaatacc gtaagatgcg ccggtaacca gtgcaacctt
    18121 accttccagt gagaaattag tgaattcaga cattgttttt tctccttttc tttataaaaa
    18181 ctgttatttt agttacatgt tccaaaggat cttatttcag atctctgttg tcaacgccgt
    18241 ccatatcatc gaagtcctga ttctctccga ccatacccca gataaaggta tatgctctgg
    18301 aaccgcatcc ggaatggatg gaccagctgg gggagatgac tgcctcttcg ttacgcatta
    18361 cgatgtggcg ggtctcatcg ggctcaccca tatagtgcat aacaaaggca tcctcaggca
    18421 tatcgaaata cagatatact tccatacgtc tgtcatgtgt atgacaaggc atggtgttcc
    18481 atacactgcc gggctctaac ttcgtcatac ccatctccag ctgacagctc tctacctgtc
    18541 cgggaaggat atacttgcag attactctgt ggttggcatc ctccagagta cccagttcta
    18601 ctttattctc atcctttaca atgaccacgc cctcttcggg agtaccttcc ggcttgatca
    18661 gcacggtcgg ataagtctta tgtgcgggag cggaattcag ataaaattta gcgggctgtt
    18721 tctcatcttc acttgcgaaa atgatctcct tggagcccat tcctatgtac atagcttcct
    18781 tgtaacctac cttgtacact ttgccatcaa cggtaatcat acctgctccg ccgatattga
    18841 tcacacccag ttctcttctc tgacagaaat actctgcacg gagttcatct ccggcggtca
    18901 gagtgatagg gcttacagga actgctgctc ctgtaatgat tctgtcgatg tggctgtaca
    18961 ccagcttgat ctcgccgggt acgaacagat tctggatcag aaactcttct ctcagacgct
    19021 ctgtggtgta atgcttcaca tctcttggtg atactgctgt tctaagttcc accttacgcc
    19081 tctctttcct caacgggcgg cctgcgtgcc gctattgaat gttacctttt tatgaaagca
    19141 cttacattat aacatatcat tgtaaccatt tccaacacat ttttactatt ttttataaaa
    19201 attttggaag gctgtaactg cttttctctt ttgtatgagt taagtatact agaaaagatt
    19261 ctcaaagtct tttcatattt taatctaaac attgcaaatt ttgaactttt ggtgtttaat
    19321 aaaagaaaaa gcgaaaagga tgaaagaaat ggaagaactt acctcctgta aaacagccat
    19381 agataactgt aaaacctccg ggacttttgc cattgcccac ttatataaag aagaaaaagc
    19441 catggacatg cacatccatg actgctacga gatctactat tccatctgcg gcggcaaaca
    19501 gtttctgatc gacaactgct tctacaccat cgccccaggc gatctcttta tcatcaacca
    19561 gtacgaaagt cacaagctga cccagatcga caactccgtc cacgaacgta tcgttctgtc
    19621 ggtggcgccg gatttcatga agctgatctc caccaaagag acggatctga gcttttgctt
    19681 cacccaccgc agtgccccct tttcccataa gctttcgctc aacaaggaac agcagaaacg
    19741 tttcctctat tatatcaaca aaatcacttc cgcggagggc tttgcccatg acatcacgga
    19801 atacgccgct ttcatggagc tgatggtcat gctcaacacg ctgttcatcc gcagtgcaga
    19861 gcagacggcg gccggggaaa ccgtaacagt tcccgccgaa tacaaggaca gtagctaccg
    19921 ctacaaccat caggtggacg atattctggc ttacatcaac cagaacatct cccagcctat
    19981 tacagtggaa cagctggccg gacagttcta cttaagcgaa tcctatatct gtcggatttt
    20041 caaatccgcc acaggcacca ccattaataa atacatcacc gcccgccgca tcagtatcgc
    20101 caaggcggtt ctgaacgaaa acggcaatgt tatggaagcc ttcgaacgca gcggtttcac
    20161 ggactacagc aatttcttca aagccttcac caaagccgtg ggtgtttccc cgaaaaaata
    20221 cgcaagcctg agtgtcagct aaactgctga cgctcaggct cttcttttta catttcttaa
    20281 tcattcggtc tctggtagaa ccggcccgcc agattctccg tacggatcat gttgacaaag
    20341 gcctgcgggt ccgtctcccg caccgtattc acaactttgc tcacctcgtc tcgtcccacc
    20401 acggaataca gcacgtcaca ttcctgtttg gaataacagc cctcaccctt gatcagggtg
    20461 gcatcgtgat gggtgatctc cgcaatagct tcgtagacat cctccggccg tttcgtcacg
    20521 atgatcagtg tctgcttctg gtagcgcttg tacagcacat ggagtacctg cgtggaggta
    20581 tactgaaaaa taatggaata cagcgccttg tcccagccaa acagtgctcc ggcgattcca
    20641 aggatcacca catttcccgc aaaaatataa ttccaggaat caatgctgta cttttccgac
    20701 aggaagatcg cgataaaatc cgtaccgccg ctggtggcat tggcgaacag acacaggctg
    20761 atggccagtg cattgatcag tccaccgaag atagcaatca gcagaatatc ctccgttacc
    20821 ggccaggcgg gcaccatatc cgtcaaaata ccgctcacca cgatcatcag acaggaataa
    20881 ccggtgaact tttttccgat gaatttgaag ctgatgacca ccggaaccgc atttaagata
    20941 aagttgatca ccgaaaaagg aggctcccag tgccacaaca gcacgctgat ctcctggatc
    21001 agtcgggtca gaccgttgaa tccaccgggg atcaatcccc cggcccggac aaagcttttc
    21061 aaattgactg ccatgataaa ggatcccgcc aggatcaggc aggtacgccg caaggtatca
    21121 caaatactct tcttcgtcat aattcgctcc ttcttacatc cttcctgcct cttccttcac
    21181 agaaccttcc tctgtgtgcg ttttttccgc attcccgtct tccatcgttt cgtctgctgc
    21241 cttttgcaaa ggctcattct ccgcacagtc tttccttttc ctgtcacacc tctgacacaa
    21301 cacgcatccc aacaggatca gtacacttcc cgcactcact aaaataccga tattccgtcc
    21361 ttccggtaca tagtgcatct gtatcgtatg ttctcccgct tccagatcaa atgccatgag
    21421 cgcattgcca aactgctcag gctctgcatt ttctccgtcg atctgcaccg tccagccctc
    21481 ctcataaggc acggacagga tcagtctgcc ggcttctttc agacttaagg ttccggaaat
    21541 atgggtgctg tccatcgcta cattttcaag atgttctttt gacaatatct ccactgcctg
    21601 tgccagcact tcttcattca gtacataagc ttccgccgat accttcgggg tatcgtccgt
    21661 gtcatcccca ttggtcagcg tcacgcgctc tccctccaac agatatccca gatacagaat
    21721 ggatccgtcc ttcagatcac tgtaatcctg cgtctccagc gttcccccaa gcacctgaac
    21781 ctttttcgtc cctgtggcat tgatcctcgc gtaatagtag cctgcccggt ccgccgtaat
    21841 acatacatta tctccgctag tctcactggt cgcccggtcc agtaagggct ctgcgattcc
    21901 cagatcctcg gtcagctgat tctgcactct cacaccggtg ccgatctcgt ccgtaacatt
    21961 ccaacccatc ggtgccacat agccaaaagg cagcgtatat ttacaatgat ataaataaac
    22021 atctccgctg ttattcacga tctcatacag accgttttcg tatttatccg attcgccgaa
    22081 catataatcc acattcagga gtgccgacac gaaagctgtc gctccgtcaa atccgtaata
    22141 aaccttgctg tgacgcatac ccagcatttt atacaggtcc atgacgttgg aattcatggt
    22201 ggaggaaaat acggaagccg tagggtatcc cgtaagagta ccgtcatttt tcgtcttgcg
    22261 ggtaaacttc tcgatacgga agaagtcatc cccctcctgt tcctgcgtaa gggcgtacaa
    22321 cgccttataa tcctcctggt ctcccagata tgcgcttcgt ccggtagtgc ccagactcgt
    22381 attaaatgta ttgatgctca gctccgccac tatgcacgcc agcaccagaa tccccagcac
    22441 ctgcctcgtc agttcgtctt ttctcgtccg gtacagatac agaagcacac catagatcgt
    22501 aacaaatacc agggtcaaca cttcaatgcc ggtgtcaaaa tcttcactct ccacgaattt
    22561 ttcacaggca agtaaaaata tcaccgccgc cagataccca tagatgatct gcctgggaga
    22621 agtgccctcc acatttcgga aagcatcata gcacatgaca aggatcagaa aaatatatag
    22681 aaaagactgt ctcgccggca gggaatccgg ataattcaga ccgtgccata aaaagtccag
    22741 gacattggtt ccgaagctga gcagcaaaaa tcccgccagt gccagattgc agaaccgttt
    22801 tctcaccgat attttttcat tcagtgcata catgggcacc agtaaaaata ctgccacacc
    22861 acagtaaatg ttcggccagt gttccagtcc cctctccgtg gtcacgcaca ggcaatgtct
    22921 cgccagcata tcaaacacgg aaaaatagga ctccagcgta ccggggaaat ccgaattgcc
    22981 aaaatccgtc cgtagaatag cacagacctc cggaatcaac aatgccgctg ccagacctcc
    23041 ggccagcagg gaggcaaatg caaatcttcc gatcctgccg aatacgatcc gtgctttcct
    23101 gctctgccct tccgtcatgg tatagtattc cgtgatcaac aacattccaa aatacagcac
    23161 cagaaagata cagatcataa tggagatgta ataattggtc agaatacaga gtcccagagt
    23221 cacggtatac atgacccatt tgccttctct gaccagccgc tctgctccta acaggatcaa
    23281 gggtagcagg atcaggcagt ccagccacat cacattccag ttgtaggccg ccagatagcc
    23341 ggacagagca tacgcagtgg aaaacaaaag agccgccgga tcctccttgc cgaacacttt
    23401 ccgcagatag atgctgaagg taagccccat aaggccgatc cgcaggatca ccagataact
    23461 catgaattcc atgatcaggc cttccggcag caaaaatacc agccagttaa aaggacttgc
    23521 cagataatag acatacagtg ccaggaaatt cgatccgatc cctacattgt aggaatagaa
    23581 cagtccctct cccgctttga tcttgtgtac aaattcactt aaaaaaggca tatactgatg
    23641 gtacatgtcg gagaacagaa aactccggtc accaaaagga tatatgcccc gtatgataaa
    23701 aagaaccagc ataataaatg caggcacaaa gaacgcaagc acagagatcc ctctctgcat
    23761 cctgcgctcc tcactacttg ttgctgtcgt tttattgccg ttcatattat ttttctccca
    23821 cgatctcttt tcttaccttc cgtaatcaaa cagcagataa tactccagcc tccggtaatc
    23881 gttcaatgct tccccacatt ccttcaccgt cgtgagtgtg ccgtccgctt ctctcacacc
    23941 ggccgcgctg atcaccggat attttttccg caccccggtc agactgctct gcagggcagg
    24001 caacggcaga tcacagttct ccagcacatc cattgccaga taattggcac tggtctctcc
    24061 gtccgtctgc tcctcaatgt caaagttgct ccagatcaca taaggtactt tatattttaa
    24121 cagattttcc tcatctgtta aagtctccgg attcacttta ttatttctta agatcggatt
    24181 gctcacatag gtcgtaggct gatgatcccc gaagaatacg atcatggtat cctcatccgc
    24241 ctgagagaag taatcgatca gaccctgcag ggcggaatcc gtctgtttta ccagtgacag
    24301 atacatggac aatgctttgg agtcaatacc atccacggta atatccgggg caaagttatg
    24361 gaattcctct tcgtaaccgc tgtgattctg catagttaca ttaaagacaa acagaggctc
    24421 tccctctttt ttattctcat acagttcgat gatcttggca aagctgctct catcggagat
    24481 atagttacgg atccgtttcg gattcctgaa atcgtcctgt gacaggaact catcaaatcc
    24541 aagcagcgga tagactctgt ctctgtccca gccgctggca taataaggat ggatcgccac
    24601 agtatgataa tccagttcct tcaggtagga agccagagaa ggcatctcct tctgcacata
    24661 ctgctggtaa gccacgctgc cctgcggtaa aaatcctata gtgctgccgg taaggaattc
    24721 aaactccgta ttggcagtat tcccgcctag cacggaaaca ttcaggtaac cggttctggt
    24781 attctctgcc ccctgttgta agctgtgaat aaagggcata tagtcttcgt tggtggtaaa
    24841 ctctccccgg actgccagat ccgaaaatgc ctcatccatg atcacaatga tattcggacg
    24901 cttcacgctc tccgggtctt ccacagctgc tgcaagccca gcggaatcct tcccggcctg
    24961 ctcataattg ctgccggtgc catctgcaga ataatctgcg ggtttctcca catccagata
    25021 ttccatttcc atcagaaatg ccacgatatt gccatctctc ttattcatga cggtaggggt
    25081 aaacagcttg tcgtataatc caaagctctg cacaaagctc tcgctctgta tcatgccggt
    25141 ataaccatag atcatgacaa tgcttaacag gatcagcgcc gctctctttg ccactcttcc
    25201 cggtgccttc atatgaaaac ggctctcgat caacaacaaa atcaggaatc ccacaatgac
    25261 cagtatcgta ctggtggaca attcatagct gaaattgccc gccacactgg ctgctgtact
    25321 gatggaataa atatcccagg gcatgatggg tgcggaacgg aaattcagca cataataatt
    25381 cgccagtcct gccaccatga aaaaggccgt ctgcaacatc agagccgccc ggacatattt
    25441 cacgattccg aataacagaa gtgcggtcag tacatagaac gccatgttca acagctgggt
    25501 tttgaaatgc attgtcgtaa aaggattatg ggtatacatt tcaaacagat aaaatgtcac
    25561 cagcggacat actgccagcg cgatacagga aggaagcttc tccctggtaa ttcctatttt
    25621 tcgaacggaa tgacgcaaaa agcctgtaat ggcagctgcg acacttcgta ttgatttttt
    25681 ctgtaaataa tccatcgctt aactctcctt acgattctga agagatttta tcgcaatgac
    25741 agaaaaataa caatttccga attgcacaca tttactaaca ttacaggctt aattttgtta
    25801 ctttttactt atattttacg tattactact tgacttcgta ttttccggct tccttcaggc
    25861 gcagcattgc tctcgccatg gatgcctggg atacatgata ctcctggatg ctctgcttct
    25921 gacgcagctg ttccttcgca cgctccaggg cagcctccgc acggaccctg tcgatatcct
    25981 ccggccgttc tgccgtatcc accagcatga tcacacggtt atgagaaata tagacaaacc
    26041 ccacacctac tacaaccttg gtccagtcgt catcctcttt ttccttaaac cgtacctcgc
    26101 cttccttcgt ggcaatgacc atatcctcat gatgggacat gatggcctgc tcgccgtcca
    26161 gagccggtac gataatgatg ccgcattttc cgtcgaaaaa tactttatca ctggcaatga
    26221 ccttcaagct gaaggtgttc atacgcacac cgcctttcct tactgtgcca tgagcttctt
    26281 ggccttctcc actgcctcgt caatggttcc tacattgaag aatgcagcct cgggatactc
    26341 atccatctct ccgtcgatga tcgcacggaa tccacggatg gtctccttca caggcacata
    26401 cttaccgggt acaccggtga agttctctgc cacatggaag ggctgggaca ggaaacgctg
    26461 aatcttacgg gcacgatata cggtgacctt atcctcatcg gacagctctt ccatacccag
    26521 aatggcgatg atgtcctgca gttccttgta cttctgcagc attgcctgta cctggcgggc
    26581 ggtctcgtaa tgctccttac ctaccacatc ctcttccaaa atacgggagg tggattccaa
    26641 aggatccaca gctgggtaaa taccctgctc cacgatctta cgggacagaa cggtggtggc
    26701 atccagatga gcgaatgtag ttgcgggtgc agggtctgtc aggtcatccg caggcacata
    26761 gacagcctgt acggaagtaa cactaccctt attcgtagaa gcaattcgct cctgcaattc
    26821 accgacttcg gtagccagag tgggctgata tcctactgcg gaaggcatac ggcctaacaa
    26881 tgcggatacc tcactacctg cctgtacgaa acggaaaatg ttatcaatga acagcagcac
    26941 gttctggtgc tcttcatcac ggaagtactc tgccatggtc agtccggtct cggctacacg
    27001 catacgtgct ccggggggct cgttcatctg accgaaaact agcgcagtct tctccagaac
    27061 tccagactcc ttcatctcac tccacagatc gttaccctca cgggaacgct ctccgacacc
    27121 agtgaaaata gaatatccac cgtgctcggt agcaatatta cgaatcagct cctgaatcag
    27181 cacggtctta cctacaccgg caccgccgaa cagaccgatc ttaccacctt ttgcataggg
    27241 agccagcaga tcgataacct tgataccggt ctccagcatc tctactacag gactctgctc
    27301 ttcgaaggaa ggtggatctc tgtggatgac ccaatgctcc tctccttcca gggattctcc
    27361 accgtcgatg gtctctccca atacgttgaa caatctaccc agtgtcttat tacctaccgg
    27421 cacctgaatt ccttttccgg tagcaatgac atccatatcc ttgcaaagtc cttcactggc
    27481 acccagcatg atgcagcgaa cggtattgtt gccaatatgc tgtgccactt ccatgaccag
    27541 acgcttgcct tcactgtata cttccagggc atccttgata aagggaagat cattgttctc
    27601 gaactctacg tctacaacag gtcccatgac ctggacgatc ttaccatgat ttttttcctt
    27661 tacgttactc aattgttcag gacctccttt cttgcctttt tcttcttttt ctgagccttg
    27721 gcaccggcaa tgacctcagt gatctcctgt gtgatcatag cctgacgtac ccggttgtac
    27781 tgaatggaca gatcatggat catggcggaa gcattcttat ttgctgcctc catggccatc
    27841 atcctggcgt tctgttcact gcagaacgat tctaccagtg caccgtatac atatcccatg
    27901 acaaaatccg gaacaatgtt atcaagcact gctttcgggg acggggtcat gacaagcgga
    27961 tcctgataca tactcagatc ctgtccgtct ttggtaataa accgttcttt tacgataggt
    28021 agcagacgca gcatctgcgg ttccgtcgtc acgctgttta ccatattggt ataaataata
    28081 tatacctcat ccagctgctc ttcttcgaac tgctccagga tcgtctctgt gatgatcctg
    28141 gcacgatgca gggtcgggtt ctgtgctgta taatggaact gctcagccac cttgattcct
    28201 ctgtgtccga aatactgacg tcccagctca cccacgacaa acagcttgta gcttcctgtc
    28261 tgtccggctt tctgcatctg ttcttctgcc attttcagta cattatggtt ataggctccg
    28321 gccagtccct tatccgcggt gactactatc agtcccgtag tcttctcccc agccggtttg
    28381 tccttccgtt catcgaaata tttattatcg atctccggta aatgacggac aattctgctg
    28441 accatcgtct gcagagtata gaagtaaggc tctgtctctt ccagattctt cttagctttc
    28501 cgaagctttg tggaagagat catatacatg gcattggtga tcttcatggt atcctgaatg
    28561 cttttcatac gactttgtat ttctctcgta tttgccatgt taacctcctg ttatttgtaa
    28621 ctattcagag ccaatgggcg aagtaaccac ataagcaaaa gtaaagccct gaaagagcgg
    28681 ttttgcgaat gggtttctga atagttacat tatttctgta aaaattcctt tttaaattct
    28741 gttgcagcat cacagatctg cttgttcagc tcgtcatcca gagccttggt ggtctctaac
    28801 tgacgaacga tgtcgctgtg ctgttccttc atgtagtcta agtacttcat ctggaaatcc
    28861 tttacctgat ttacaggcac atccagcatc actctgccgt tggctgcaca cagggtcata
    28921 acctgctctg ccatgctcaa aggtctgcac agaggctgct tcaacagttc catcagtaca
    28981 tgtccgtgct gtaactgttc ctttgtggcg ttatccagat cagatgcgaa ctgggtgaac
    29041 acttccatct cgcggaactg tgccaggtcg atacggatac ttccggctgc tttcttcatt
    29101 gccttggtct gtgcagcacc gccgacacgg gatacggaca gaccgacatt gactgcaggt
    29161 ctcatgccgg agaaaaacag atcactctcc aggaagatct gaccatcggt aatggagata
    29221 acgttggtcg gaatgtaggc ggatacatcg cctgcctggg tctcaatgat cggcagtgcg
    29281 gtgatggagc cgccgccctt atcggcactc agtcttgcgg aacgctccag cagtctggaa
    29341 tgcagataga atacatctcc gggatatgcc tcacgtccgg gagaacgttc cagcagcagg
    29401 gacagtgcac gataagctac cgcatgcttg gacagatcat cgtatacgat gaggacatcc
    29461 ttgccctgat acataaagta ctcagccagt gcggtaccgg aataaggtgc aatgtactgc
    29521 agaggtgcag gatcgctggc ggttgcagat acgataatgg tgtaatccat ggcatcgttc
    29581 tttttcaggg ttcctaccag ttttgcaatg gtggatgcct tctggccgat agccacatag
    29641 atacatacca catccttgcc cttctggttc aaaattgcat cgatggcaat ggaagtctta
    29701 ccggtctgac ggtcaccgat gatcagctca cgctgtccac gaccgatggg gaacatggaa
    29761 tcgatagcca gaataccagt ctccataggc acgcctacgg actgtctgtc cacgatggaa
    29821 ggtgccggct gctcgatggg acgatagcct tcttccttaa taggacctgc accgtcaatg
    29881 ggtgctccca gagcatccac gattctgcct ttgaatccgt cacctacagg aataccggct
    29941 ctcttaccgg tacgaacgac tctggtgcct tcgcggatct cggtatcatg accgaacagg
    30001 atacatccga tctcatctct gcggacatcc tgtaccatac ctttgacacc ggaatcaaag
    30061 ataacgatct cgccgtactc tgcatggtcg ataccattga cgttggcgat accgtcacct
    30121 acccagctga cagaaccgat ctcattttcc tgtgcctgta agttgaaatc ctcgatctca
    30181 cctttcagaa tggaaataat gccctgctca ctggcggtct ttcctacctg ggaaagcttg
    30241 tctgcgaact gcttcatacg tccattggta ctccagtcat agacctcgtt accggcacgg
    30301 atgatgaaac cgccgccaag ggaaggatct tctttggtct caaagaccat atcctctctg
    30361 ttgtatttct tctggacaaa attacggata ttctgcagct gctcatcggt gggcgctgtc
    30421 acataggaca gggttacaac gaactcttcc tttttctcgg gagagacttc tctgtatgcg
    30481 gtgcagatat catcccacag atagacgtct ccgttgtcac acaggacttt cagaaaatct
    30541 ctgatctgta aggggaatac tttttctatt acattgtgct tggcagacag cgacacagca
    30601 ggggattcga aatccagctt cacctgcggt acggctttga agatatcgcc tgtctgacct
    30661 acaatgtctc cggggatatt cagttcacgc aatctttttg cgtattctaa agctgtacta
    30721 atcaatcttt tcacctgtct ttgctataaa ttggttgtaa agttctctgt ctgaatctgc
    30781 gttctgcttg gcagctacca gttttgcagt agcttccact accaagccgg cgatctgttc
    30841 ctgtgcctgc tcgatgcgct tctgctcttc cagtgtggca ttcttactgg cattgtccat
    30901 gatctttttc gcttctgtct gggcatctgc cacgatgcgg tcatattcgt tgctggcctt
    30961 gcctcttgcc tcgttcagaa tgctttcttt ttctgcctga acaccgctta aggatgcctc
    31021 gtactggcct ttcagttcat tggcactgtc ctgtgcagct tttgcatctg catattgctt
    31081 atcgatctct gcctgtnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    31141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnggca
    31201 ctgtcctgtg cagcttttgc atctgcatat tgcttatcga tctctgcctg tcttgcatcc
    31261 aggatcttgt ggatgggctt gatcaggaag atcttcacaa tggcaaagat gatcaacaga
    31321 ttcaggatgg tccatacaat attaatatct aatctcagca tgtgctttca tcctttctta
    31381 cgtttcgctt tctaatttcc ttataactat ccgaaacttc cattcgcaaa acgctctttc
    31441 agagctttct ttttgctcat gaagttttag tcatcgtaaa tcgtcacaaa acagcttaca
    31501 aatgcaggca tttgacgctt gtctgtgacg catccgactt cggatagtta tgccatgaac
    31561 agaatgatca ggataccgat aacgaatccg taaatagcgg ttgcctctgc cagcgcacca
    31621 cctaacagta agatcttggt gatcttgctc tctgcttccg gctgtcttgc gactgcctct
    31681 gctgctttac ttgttgcgat accgataccg atacctgctc cgatacatgc cagtgctgca
    31741 atacctgctc caatagctat cataattctt ttcctccgtt tttgtgtgaa ttagtgtttc
    31801 ataactattc ataatcgcat tcgcaaaata gctctgaata gttatccagt tactcaatgg
    31861 cttccttaat gtacatggat gtcaggaata caaatacata tgcctgaatc aatccatcaa
    31921 aaatatcaaa gtaaaagctg aaaggtaccg gcaatgctac cggaaccaga agcttcaaca
    31981 gctccatgat aacgaatgct cccaaaacgt taccaaacaa tcgcatacac agggacaggg
    32041 gcttgatgaa cacctccagg atattgatag gtgttacgat cgcgatgggt tcggtaaagc
    32101 tcttgatcca tccgcccaca ccttttttgc ggatacctgc ggcttccacc agaacgatac
    32161 tcatcagggc cagtgccgcc gttacgttca gatccttggt agggggcttc ataccgaaca
    32221 atccaatgat attggatata cccagataca acagtacgct aaccagatac ggtacatacc
    32281 gtttgccttc ttctccgatc attcccgtaa caatgccttc cagctttgtc acgatcgtct
    32341 ctgccaccag ctgtcgtctg ctgacattgt gtactttgag gttgcttgta aggataatcg
    32401 acaagatcag aatgactgcc atgaccaccc aggtgataac gaccgattcc gcaatgtcca
    32461 gttgtactcc accgatcagg ggtagacctg ccggaagctt aatagaaaaa gccgtgtcac
    32521 aattcagttc cttgagcagt tctgatgcca attcctccat aataccactt cctttccgtg
    32581 aacatccttg aaagagcttt cactccctca cggtcgttgc tattttactc ctatttattg
    32641 aaaaacgtaa gatacatttt tgctaatttg ttccatgcaa aaatgaaaag ttatggcata
    32701 tattaactat aaaaaatgag gtatcagaaa atcatatgac aaaattagtc attttgggac
    32761 gcaaatccca gacgggcaat tatgagacgt ttttttcgaa acttcccgcc agaaccgtaa
    32821 ccacactggt ccctggggaa gtcacctcct gtgatgccct gattctgccg ggcggcggag
    32881 atatcactcc ggctttcttc ggagaaaaaa acaaaggttc ccgtactgtt gacaccgaac
    32941 tggacatcct gcaatttcag gcccttgaca cagccattgc cgccaggatc cccgtgatcg
    33001 ggatctgcaa gggcatgcag gtcatcaatg ttgccttcgg tggcactatt ttgcaggata
    33061 tgtcgggaga ttccctgcac agtatccctt ccggtgatgc attccatacg tccattatga
    33121 cggaagattc ccgtcttttc aagtattttc ctgtcaaaat gcacgtcaac agcgcccacc
    33181 atcagaggat cgaccgctta ggtcagcatc tgaagatcat tcagcgctgc cccgatgacg
    33241 gctgccccga agccttggag catgacgagc tgccgatcct tgggctgcag tggcatccgg
    33301 agcgtcttcc ggaacaggcc ggtgagcctc tgctttctct tctttcttct tatttctgaa
    33361 tctgcgcccg ggctttttcg ccggggccag ctcctctctg accttgactt tggcgatttc
    33421 gaacagggag cggatgattc cccgcagcat ctccgcggtc tggtcgtcca cttccttcag
    33481 tgcagtaacc accgcattca tactcttttt ccagtcagcg gtaaacgtga tcagatcatc
    33541 cgcctgggat gcggagatga cctgatagag tgtttccacg aaggtgcgga cctgttcttc
    33601 atttaaggac agtatccatt cgttcagtgc ttcatttatg atcttctggc tctcataaat
    33661 gtcacccaca tccacgaaat ggtcctcctc caccaaccag gaaaaaggat cgtgctgcaa
    33721 aagcccatgg ctgttgctct ccaccacgcg gtaatgaata tcccgctcga aaatcatacc
    33781 gatcatggaa gaatgtggca acagcttcac caccttgcct tcgatggcat tatagtgaca
    33841 ctccttcagc acttccggcc ggaatccagg cccatccaga ctgtagatct tctggatccg
    33901 tttctgcaca aaaggggcgc atttcatgcc actgtagact gccagattgc cgcccttgga
    33961 atgccctccg atataaaagg gctggtgtag ccatccggtg accatattta catattttac
    34021 actgtactcc tgccccggca ccggtgacag aaatgccatg ttgaaatctt ccttccagcc
    34081 tatgatagtc tcatccgtcc cccggaatgc cagaaacagg gttccgtcat ccagaataaa
    34141 agtgatggcg gagaactggg tctcccattc cttttccacc agattgatat agcagttcag
    34201 cttcatatcc cggaaacgtc tgccgcttaa cattccttcg aacagcgccc ggttctcctt
    34261 ctcaaaacgg acatccccga acagtttgtc cacgtccgga cgctctgcaa tctcccgcag
    34321 ggtcacggaa gggccattgt gccgcacatc gctgaccatc ccgtcgaatt tcagataaga
    34381 gagctggcac aatgccagac tgtccacatc attcagaggt ttttcccgaa aagaaatgtc
    34441 tccgtatttt tgcaaatatt ctattaccgt tcctgccata tgccgctcct ttccgcgagg
    34501 aactttcctt gcgtatttcg ccggattgtg ttacgttcta tataatgatg ccagggtcaa
    34561 aaggtctaag cccacatgag cttgctcata ggt
//
LOCUS       DAWDHD010000008        32400 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_115_length_32400_cov_11.529726, whole genome shotgun sequence.
ACCESSION   DAWDHD010000008 DAWDHD010000000
VERSION     DAWDHD010000008.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 32400)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 32400)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..32400
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115_length_32400_cov_11.529726"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         7856 a         8558 c         6760 g         9226 t
ORIGIN      
        1 tggtgttgat gatctccgca ttcaggtcat cgatcttctc ctgcaccaga tcctgttcat
       61 cttgcaggga attgatctgg ctgttgatat tgttcagctg gttctgatgt tctttaatgt
      121 tgttctgcag atcactcatg gaaggtgctg cctgcaccgt ctccgcggga gattcccaga
      181 cagagccggt ggctgtcagc gtcaccgctg ccagaagaaa ataacataaa aactttcttt
      241 ttgctctatt tacttttctg ccggcagttt ttttcttcat aggtaaaatg actccatata
      301 attacaggtc gcagttattt acggtacgag ggaaggggat aacatcacgg atattgccca
      361 ttccggtgat atacattacg cagcgctcga agcccagtcc gaagcctgca tgacgtgcgg
      421 agccgtagcg gcgcagatcc agatagaact gataatcttc cttattcagt cccagctctt
      481 ccatacgctt ctccagaatc tccagattgt cttcacgctg gctgccgcca atgatctcgc
      541 cgataccggg taccaggcaa tccatagcag ccacagtctt gccatcctcg ttcaacttca
      601 tgtagaatgc cttgatctcc ttcggataat cggttacgaa tacaggtctc ttgaattcct
      661 tctcggtcag atatctctca tgctcggtct gcagatcgca tccccagaat accttgtaat
      721 cgaactcatc gttgtgcttc tccaggatct tgatcgcctc agtgtaggtc acatgaccga
      781 aatccgagtt gaccacatgc tgcaatctct cgatcagtcc cttatccacg aactggttga
      841 aaaatgccat ctcttcggga gcattttcca atacgtaacg aataacatac tttaacatgc
      901 tctctgccag gatcatatca tccttcagat ccgcaaaaca gatctccggc tcgatcatcc
      961 agaactcagc cgcatgacgg gtggtgttgg aattctcggc acggaaagtc ggtccgaaag
     1021 tatatacgtt cttgaatgca aaagcataag tctctacatc cagctgtccg cttacggtca
     1081 ggtttacagg cttaccgaag aagtcctggg tgtaatccac tttgccgtct tctgtcttag
     1141 gtacattgtt catatccaga gtggtcacct ggaacatttc accggcacct tcacagtcac
     1201 tgctggtgat cagaggagta tgcacataga caaatcctct ctccatgaag aaggtgtgga
     1261 ttgcataagc gatcagggat ctgacacgga acactgcctg gaaagtattg gtacgtgctc
     1321 tcagatgaga gatggtacgc agatactcaa aggaatgacg ctttttctgt aagggataat
     1381 ccgcagtaga aggtccttcc acgatcactt ccgctgcctg catttcaaat gcctgctttg
     1441 cctcgggggt agccacgatg gtgccgcgga tcacaactgc tgcacctaca ttgatcttgc
     1501 agatctcctg gaagttcgca agtccatcgc catatactac ctgtaaaggc tcaaaaaagg
     1561 ttccgtcgtt gatcaccagg aaaccgaagt tcttggagtc acgattgttc ctcacccagc
     1621 cgcctacggt gatctccttg ccgatataga cgtccttttc ccggtgaagt tctctgatgt
     1681 ctgttaattc cataattttt cctatccgca cgctgcgacg tgctctatct gaaccggccc
     1741 gcatcctatt cgccggtcac atactatttt taaaatttaa ttgttggtga tctttgcgtt
     1801 ctctaccaga taatctgtca ctttatcata gaggaaatac tctctgtagt cttccttgga
     1861 ggtctcgccg atatactcct cgatggtgtc ataaccggct gctgtagcac gatccagcag
     1921 gatctgatcc agttcttcat cactgatgtt caggttttct ctgttggcca ctgcctgcaa
     1981 ggcaatatcc tgctttgctg cctcctcaga ataggtagcc aggaaagatg ccagatcctg
     2041 tccgtaataa tactgcgtga acgtatctcc gtccacaccg taagcagatg ccatggaggt
     2101 aatgctgctc tccgctgcat cagaatattt ctgtaccaga gcctccggca cggaagtaaa
     2161 ggtgttattt gccataaacg catccatcac tgcctgctgt acattggttt cataattctg
     2221 ctgtgcattc tcattcagat aatcataagc gtactgacgg agttcttcct cgttcgtcac
     2281 accatcgata ccgaagttac tgatgacttc atcactgaat tccccatcct gtgcgggctg
     2341 aatataattt accgttaccg taaataccac agcctgtcct gccagatcgg attttccata
     2401 attctccggg aatgtaagat tcagatccac tgtctctccg ggcatcacgc caatcagtcc
     2461 atcctcgaat ccggcaataa agcttccgga accgatggtc agatcgtatc cctgtgcggt
     2521 accgccgtca aatgccacgt catccttttt gccttcgtaa tcgatgttta cggtgtctcc
     2581 ggtctccaca gcacgatcca taatcccgcc gttctctgcg gtaatatttt cataatatac
     2641 attattgacc agactgtccc actcatcctc atctacttcc acggtgtcta cggatacttc
     2701 cagccccttg tactcgccca gtgtcacata cttgtccaca tccatatcct tcaatgcagt
     2761 actttctgct ccggcgccgg tcgctgcatc cgtgccatct cctgtatcct ttgctccgca
     2821 gcccgccagc atacacatgc ttaaaaccgc tgccagaaat cctaatatct ttttcttcat
     2881 tctaatactt cctatacctt tcattttgtg cctaccatgc gaaatgcatc atagcaccgc
     2941 ccattataac acatccaccg ggctataaca agggtgacaa taaccggcag acctgctctt
     3001 tgatacggat tttcaacctt ctggccacat aataatcccg ggtgatctgg cggcagactt
     3061 ccaggtcttt ttgaaagata tccaccattt cctgtgcttt tttctcatcg tagatcaccg
     3121 catttacttc aaaattcaac gcaaaactgc ggatatccat gttcgccgtc ccatagcaga
     3181 gcacctttcc atccacgatc atgcctttgc tgtggagaaa gccgttattg taagtatagc
     3241 agttcgctcc cgccatgacc atatcgccaa gataagaata ggtggcccaa tagacaaacg
     3301 ggtgatccgg catacatggg atcataatgt tcacttcgat cccggacttc accgcgatca
     3361 tcagggcatt caggattgcc tcatccggga taaaatacgg agtctggata taaatgcttt
     3421 tctgtgcctt gttgatcaga cgcaggtaat tatcatggat ctgctgagtc gtcttatccg
     3481 gaccactgct gataatctgc agatcacaga aatccctcgt accggcttcg atcccccgga
     3541 acagttccgg gcgcaaaaac aggttctctc ctgccgcata attccagtct aaaataaatc
     3601 gcacctggag ccccagtacc gcactgccgc aaatccgcag atgggtatct ctccaatgcc
     3661 cgaatctttc atccagatca atatattcct tccctacatt gaatccgccc acataggcca
     3721 ccttattgtc aatgactacg atcttgcggt gattacggta gttgatacgt agctggagtc
     3781 ttcgcaggat cgccgggaaa aactccgcgg tctgcacacc cttttcattc agccatttcc
     3841 agtagctgtg ccgcacggaa cgacagccca tggcatcgaa cagaacccgg acctccacac
     3901 cctgtgccgc cttctcgatc agaatatcct tgatctgatt gaataatacg tcgtttttaa
     3961 tgatgtagta ttggacatgg ataaaatgct ccgcattccg cagatcttcc atcagcgccc
     4021 ggaatttctc attgccgtcg ataaagatat ccacatcgtt gtccttcgtc agcatggctt
     4081 ctccggcctc cagattatac atgaccagat cctcataccc ccggatactg tcatccattt
     4141 cctggacatc ccgggatctc agccgatact cctgctgtct tatatcttta ctgatgtgat
     4201 cctccacctc tttgatccgg aacattttcc gcttgtgcat atcctgcccc agcagcagat
     4261 agaacacgaa ccccagtacc ggtataaaat acaacaaaag cagccatgcc cacacactct
     4321 ttggatcccg tctctgaaaa aacacgatca cgatggcaaa cagcatatta aaaaatacca
     4381 ggtggttgca gaaaaaatct attcctgtct ttacgatctc aaatacttca ctcataatac
     4441 tgccccttca gatgaaaaac atcccttttt ctcaatatat cataatgcaa accttctttc
     4501 cagcaaaaga gactgacaaa tgccgaaaaa atcaacattc tcagtctctt cttatctttt
     4561 gtattcaagc cggcggtctc cggccgtgat attttatccc tctgtgatga gtgttctctt
     4621 tctatcctga tcacagaatt caatcacata ggaacaatcc tgcagatctg ccttcagctg
     4681 tgcagtacag ccctgatgtt cccatacaaa gcagattctg gatccttcac tgcttacttt
     4741 gatccggctt tcttccgaga acgccgggca ggaattccgg aatcgcagca actccagttg
     4801 ctgctttaca atcggcttac tcaaaccact gcatatctgt gctgtcgtca gatttgtccg
     4861 attgatctct ttatgtcctc cggggcctgc catcttcacc gcttcataat cgttctttcc
     4921 ggcgaacaga tccagatacc agatctgcgg tttgcccggc ataaatatct gcaaggctct
     4981 cgcaaggagc atctttctct catcttcgcc caatgcgctg aaataagttg cattcacctg
     5041 atagtaaatg tttttctgac cgtgaagatc cttcacatat cccccacggg agacaataat
     5101 gtcgatcagt ttctgaatcc ggtcctccgc cagaattcct ttgagatcca acagtggaat
     5161 tccatcgtga cagccaagca tgttcacggt tctgatattt ttatcgatca gctcctgtgc
     5221 ccatccggcc agatgttctc cgttccccga ttccagggca tctataatga gtcccggcag
     5281 aaagaagtca taggtgacat atccctgctc tgctatcttc tcatatattt tttcactata
     5341 gctttcatgg atctccggca gaagtgccat gccgtacggt tccgcaatct gttttatttt
     5401 gttcagcagt tcccaggtgt ccggctgatt caaaaaattc cgttctcccg gtttcttcgg
     5461 tgcataggca aatgcatcca gtctcacaat tttggcacca taccctgcca gtgtctttaa
     5521 tacttcacgg tagtactccc acacctttgg cgactggata ttcagatcca tctgaccaag
     5581 ataataccgg tgctgctcca tgtaattcca gatcgttgta atctgttctt ttgtcagcct
     5641 tcctgctttt cgctccagaa cgatttcctg caaaatatcc gccggctttt tcccttccgg
     5701 acagcccatg gacagttcct gtgcaatgat ctctgcctgc atgtattgca gatcggcagc
     5761 tttcataagt tcctgagcac tgactttggg atagttcact tcctgataaa aagtattcca
     5821 gtaagggatc tctctcccat ccgggaaacg caccataaga atggggagtc ccggtttccg
     5881 gaaaaacatc tttttcagat acttttcttc cggaaggata tatccctgct ccgtcatttc
     5941 tccgcagtcc ttccagaatt cattccaatt aatgaagaag tccttgtact ccgattcttc
     6001 cccttttgca atgatatcct ggaattgctt tgacaacacc gaagcatgat tcagaataaa
     6061 atccagcttc aattcaatcc cgagcttttt tattgcttcc aatgtttcgc cggaagcata
     6121 catcttgttc agagaataat caatcacaga aaagcctctg tccagatccg tatggtacag
     6181 actgggcaga atatagcagg aagaaaatgc tccctctgcc tcttccgttt tcaaaaaatc
     6241 tacaacatca tccaaggttc cgcccagact atccgggtag agattcaaca tcggtcctat
     6301 gttcataaca tctttccttt ttatttctga agcttcggaa caaattgcgc atattccttc
     6361 cggtctaaca ccgttccgtc ccgggtaaca ataagttttg ctttctcagc ctgctttttc
     6421 agcattttct gctcaatatc cagtaccatc atcgtaaatg ggttgtctat ccattgatca
     6481 cggtttcttt ccagacggct ctgcaatgtc tcctgggggg tactgtcaat gtagataggg
     6541 atatccacac cgctgtagta ttcactattg ctgtgtgtcc actccaccaa cagcactttt
     6601 attccggtga aatccttctc ttcataccac agttctgtct cttcgcgtcc catatgtctg
     6661 acccatagct tttctccgcc ggctttaaac gcatgaacca atctgtttac ttcctcaaaa
     6721 ttaatttcct tttccgtgcc aagataattt tccagagctt ttctgccgtt tctaagatag
     6781 gactcatacc atggatattt cccgacatgt ttttcatccg catcatttcc ggctttttga
     6841 aattcctgaa tgattgtaca tcgttcttct gtataagtct gctctttcag cattcccaca
     6901 attgcatttt ctctgaaaat ctgtagccgc tccgcatcat tgtacttggg aattctatgc
     6961 ggataagcat ctccggacaa aatgtagttt tctatccctt tttccttcag gaagcaggaa
     7021 agtaacaatg ccgtcactgt ttttccggat ccggagcctc cgcttactgc aacgactact
     7081 ttttcggaac gtgtctgctt cgcacagttt tcaatcatgg ggagcagctt ttgcatcagt
     7141 ttttctgcca acgtagtatc atatattttc agtagctcct ttgatgacgg ttctgcttca
     7201 ctttccatta tggttccttt ttctatcata tctgttcctt tatcgtcatt attttacttt
     7261 gtggactccc ggtcgatcag ttctaccgga aatacaacct gggatggaac cttttcactc
     7321 tttacttttt tcacaatcgc ttctatggca tattcacaca tttcctgcac cggctgatga
     7381 atggtcgtaa tggaaggata cgtcagtgtg gaaatccagg tatcatcgaa tcccacaata
     7441 gagatatcct ccggaacacg ttttcccatt tttttgcatg cacggataat atttgcagcc
     7501 attacatcac ttgttgcaaa aattccatcc agatccgaat tttttttcaa taattccaaa
     7561 atattgttgc cgaattcttc tgataaggga gccaacctgt ctgtataaac cacacgtcca
     7621 ctgatttttt tatcctcgca gcacttcata aatgcctttt ctctgctata tgcagggata
     7681 ttttccttgg taggcgggtt aaaaatcgca atatttctgc atccggcatc aaacagccgg
     7741 ttagccgcca gaataccacc ctgatagtta tcgcaaagaa ctgccggaat gttttccgca
     7801 atttctctct caaaagagat cagcggacag ttagaatcca gatgttccat cgtctctccg
     7861 atatggctgc acacaacaat tcccagaact ctgtgagaat agagcagatc aatatactgt
     7921 aattcttttt ccctgctgtt ctcggaaata cagaccatta tcttgtagtt gtgttttgtc
     7981 acataatgct ccagccagtg aaggaccttg ctaaagtaag gatgctctac agatgggaga
     8041 atcaccccga tataatcact cttctgccgg gataaatttc tcgccatttc gtttggcata
     8101 tagttcagct tttccatggc agaatatact ttttctctcg tctcttcact gatgtatcca
     8161 cgattattca atactctgga tactgttgtg gaggtcactc ctgcttctgc agctacatct
     8221 ttgattgttg gcatattccc ttccccttat cttacttttt tgcatcagac agtaacggaa
     8281 agtccgtcgt aagtcaaaag gaacccctcc tgacctacca gtctttcata ttcctgcgga
     8341 atcataccgc acatatgaga ataatgattc agataatatt ccgtatccgg tgtcactgca
     8401 tgcattctgc ggagtttctc ccgcagctct acattttccc gaattcccat atgcgtgtca
     8461 ccatccagta tacctctgct ggtatccata ctgacgacgt caaattttat gttctgttgt
     8521 tctataaaat ccagtgtttc ctccggcaat gcccctgtat catttgcata caacagggtc
     8581 gagcttccat cctcgatcac aaaaatcaaa gcttcctcgt ccagcttgtg atatgccttt
     8641 aaaggtgtaa acttcaaatg acctatccgg aatggttcaa acttttgaat ccggcgaaat
     8701 ctcatccttg tcagatctac atgtggatcc ttagctatta tctccataat accatcccga
     8761 accgcatcat tgccataaat ttccattact tttttatctt ttttttgaat cacggatgcc
     8821 atttcccgca gtcggaagca tatttccgtg gtattcaggt gatcggaatg agaatgggta
     8881 aataccaggt gatttacctc cgataaatcc acatggctgt taatacattg ggaaaaaata
     8941 tccggcgaca ggtcgatcat cacctgatct atcagagtac agctacgtgt ctttacattt
     9001 tttcccaaca acgttcttac cttacgacat gcttcacact ggcagaaaat actggggaat
     9061 ccttctgctc cgcctgttcc gagatatctg atttccactt tatcacccct taattccgct
     9121 ggatgtcaca gatgccacaa actgcttctg gaataccagg aataatatca tcgtaggaat
     9181 aaccattaat gtagagaacg caaagacctg tccccaatca gtgtaggtat cggaaaacag
     9241 tgactttaaa gccagcggca gtgttctgta agtaccgtct ctggtaacca gtgtgggcca
     9301 cagcagatcg ctccaggaac ggatgaatgc catgatcgca acagtggtaa tgatcggctt
     9361 agacaaaggc atcatgattt tggtaaatat tttccagtag ctggctccat caataattgc
     9421 agcctcctgc aattccttcg gcattccctt aaaatgatta tagtatagat acatgtacat
     9481 gggatatgcg acactgggaa tggccagtcc tatatatgta tttaacattc ccagtgaatt
     9541 cactgtcata aaacggttaa tgataatgga ctccgtagga ataatgctca atgccacaat
     9601 ggtggatata atgattgcct ttccacggaa attaagcatt cccagtgcat atccgatcat
     9661 tgcattgatt gtggttgcca gcaggacaaa gatgatcgtt actacaacgg aattcttgaa
     9721 gaacttgaag aaatccgctt tcacaataac attgatatag ttatccagtg acaggtctcc
     9781 gtaaggaatg aatgctctga tatttgacat atccgattca atctgcgcat atggtttcag
     9841 ggaagatacg aacaaaaata cgatcggtcc gatgaaaata gctgctatga gcaaatatcc
     9901 aagatacatg cacaatttca taatgatttt tttccttttt gacattgcag ttctcattcc
     9961 ctagtcctcc tttgcaaact tattctgcag aattgaaata accattacca gcataaagaa
    10021 tgtaactgcg attgcggaag agtatcctac ctgtccattt acgaatccgg ctttgtacaa
    10081 ctggaatact acggtcgtcg ttgcattgga cggtccgccg tttgtcagta catacacctg
    10141 agtgaatact ttcagggaat tgataatcac agataccaaa acgtatacca gtgtgttctt
    10201 caatccgggt accgtaatat aaataaattt ctcaaaggtg gtacatccat caatttcggc
    10261 agcctcatat agttcttcgg aaatgtactg cagaccactt aaaatgatca acatctgcat
    10321 accaagattc tgccagatat acataattgc cagacaaggc agtgccagat gctgatcctg
    10381 aagccacttc tgaggtgcaa tatgcagcag acccagaaac gaatttaata atccgctgga
    10441 attggaggaa aaaatgaatg cccagataac tgccactaca gtcatcgtta caacctgcgg
    10501 actgaaatag atcagtcgga acacctgtac gcctttgatc ttctggttta caaaaaccgc
    10561 aagcacggtt ccgatcacca ataccgaagg cactacaatc agtgcataca aaaatgtatt
    10621 tttaaaagaa agtactgtca ctttgcttgt gaaaatctca atataattct tcaaccctac
    10681 aaaggctgtt tccattccca ctttgggaat cagttgttta ttggtaaatg aatacacaac
    10741 agacattgca aacggtaaaa tgataaatac cagtgccagc accactgccg gtgtgcagaa
    10801 caggaatgcg gcttttgact cgccacgttg caatccgctt tttttcttag aactgtttgc
    10861 cataggtact cctttttctt atgaataagg ctgccctcac agacagcctt atccgatcct
    10921 tcttatttca gattattgaa agttccatcc gttatctgta ataatttcat cgatatttgc
    10981 agcagcatcg tgtaattctg ttgcaggatc tgcaccggca tagatattac cgataacgct
    11041 ggccattgca tcatagatgg tcatatgtgc aggagtgtaa ggacgcagga aggaaatacc
    11101 actctccagc tgctgtctgt acagatacag ggtacctccc tcacaatagt ccggaattgc
    11161 atccataacg ctggatcttg cgggaactgc accgttgaca tctacgacca tcttgatgta
    11221 atcggggttg gttgcttcct gcagcattgc ccatacagca tcatcatttc cggattcctg
    11281 tgctcctgtg gtcatgatca taacagtgga accggagcag gtgtaaactc cgtgccccat
    11341 atcaggcaag ggaaccagga tcagatcatc gccaagtgca gccttataat cctgatactt
    11401 ggaatgtcca tacagcgcca gggcattctc acccttctgg aaagaatctt cataatcaca
    11461 ggttgcatct gcatatccat ctgtgaacag ctttgccagg aaatcgaaag aagcaatggt
    11521 attatcgccg tccagggttc cggtgcaaag tccggtctca cgattcatgt agtctccgcc
    11581 aaagcttgct acaaacggca tccaggtgta atactcggtg gaaggtttgt tctgtcttac
    11641 atagatgggc cattctacac cgctgtcttt taatttcttc agcgcatctt caaactctgc
    11701 tttatcccat gcatccttgt aatcggcagg aattctgaca ccggcattct ccagcatgga
    11761 tttgttagcc cagaaagcca ggccactatc gaactgtgca acggaaatga tgtgatcatt
    11821 ataagtgcac tcagatacta cgctgggaag ataatcatcc atcagttcat cagttacata
    11881 atcatccaga tttgctacca agcctgcttc gtagtatttg ggaatcagca gatagtcggc
    11941 aactacagca tccggcatgt ctccggcgat agaagatgtc tgcatcttgt tatccagctc
    12001 gtccttggaa taatactcat ctgtaatggt aatattcaga tcaccttcaa tcttgtcaaa
    12061 catttcacgg tatatatcat cttctgcgcc ggagctgatc cagactttca cttctttccc
    12121 ttcataaccg ccctgtgcat ctgttgcagc ggctgctgtt ccatccgcag tatttgccgt
    12181 tgtttcactc ttggagtctg tagttgtggc acctgtctct gagcttccac aagctgctaa
    12241 ggatacgacc atcattgcac tcaacaatcc tgctaaaatt ttctttttca tgtattgttc
    12301 tcctctctga atctatacat tttgtagata tgtaattgta tgtcaaccgg ttacatattt
    12361 tgtaattaag aatatatact aatttttatc gtgtgtcaac cggttacata tataaatttt
    12421 caacaaactt ggatttgatt ttttgtatag tttcactttg tactccaatt tgacgcagat
    12481 attctggaac tccaccccat tcctggtcaa tccagttcag aatccatttc atgttgtaga
    12541 tattggaacc ggaaatatcg gtcgtaaatt ttgtaatata ggtgctgctg cattggtaat
    12601 ccaccaggat atcttccctc tccactccca cggatgataa gatcagcatt gtcagaatgc
    12661 cggtgcgatc cttgcctgct gcgcaatgga acaatactgc tccctcggaa tcagcgacta
    12721 cctccagagc tttcctgatc tcttttcctc cgttgagtag catcaggcga tacatgcgat
    12781 gcagcgtttt ggtctccacc acgctactgt ttacaacatc cagctgttcc agctttgtgg
    12841 tacccataag cgaacagttg atataatcca tatcctgccc caatctgtcc ggcagttctt
    12901 ttgtctcact gggaaatctt aagtcaatga ccgtcctgac gccaagatgc tcaatcttcg
    12961 caatatcttc ttccgttgcc ttatttaagg ctgtactccg aaaaatcaca ccatatctgg
    13021 ttacaccacc gtcacacgca tagcctccca gatctctgaa atttgccacc tgatccagac
    13081 gatatctcct acaattcatc tctttccatc cctttttatc tctgttatta ttattttatt
    13141 ccttattcca acggttctct tgaggtcttt gcatccccga aaaagaacat aaaatcctga
    13201 atttctgttc tcatcatttt atcagtactt ataaattcct ttgccttttc aaactgtggt
    13261 ttcctgtcgt agggaatttg tttcaatcgg atctctccgt ctgtaatcgt cgcataatgt
    13321 gccgtcttat ccgggtcatc gggtcttcga tacgaggcac ttcccgggtt cagccattcc
    13381 attccatcac ccagaatatg aatacattgt ctgtgtgtat gcccgaaaat cagtcttctg
    13441 ttccctgtat tatctgttcc gtaatgctcc gcccaatatt tatcaaacgc gtacctgctt
    13501 tctatcgtcc catagccact atcatactga tgggcaattc cgtatttcca tccgtctgcc
    13561 tcaaaatatg cagtttccgg taactgcttc agccacctaa taccctggtc gtttagttgt
    13621 tcacaattat agtgaaccca cttgtatttt ccatcttcta catttttcca ttccttactt
    13681 tcataagtat ttataacgtg aagatcatgg ttgccataca caagatgcgt gtttttcaga
    13741 ttgcggaacc gttctatcac ttccgccgga tagattccat agtctgtata atccccggca
    13801 cagcacagaa tatcgtaatc agcctctttt tccaaaatgg tgttcaatgc ggctatgttc
    13861 gcatgtacat ctgatactac cagtactttc attttccgca cctccttctt ttttcacatt
    13921 atataatttt accgctcgta tgtcaaccgg ttgacatacg tgtttcataa ttttttataa
    13981 tacaaaaaga ctgccacatg gtgacagtct tccttacaag aaccggaaac aagatagaaa
    14041 tcataaatgt atggttgcgt atttcatcac atctatgtta caatgttttt taggaatttg
    14101 ttccgattaa ctgaaggagg gactgtaaaa tgagtccggt ggcaatcaca ttattagtta
    14161 ttgtaatagt acttgcgatt atcattgcag tattatattt cttaggtaag aaagcacaga
    14221 agaaacaggc agaacagcag gaactgctgg atgccaacaa acagaccgta tccatgctga
    14281 tcatcgataa gaaacgcatg aagctaaaag atgccggtct tccacagatg gtcattgacc
    14341 agaccccgaa gatgatgcgc ggttccaaac tccccatcat caaggcaaag gttggtcctc
    14401 agatcgtatc cctgatctgt gacgagaaga tcttcgacag cgttcctgtg aaaaaggaag
    14461 taaaagccgt tgtcagcggt atctatgtac tggatgtcaa gggactgcac ggcaagaccg
    14521 aagctgctcc cgagaagaaa aaaggcttct tcaagcgtct tgtagctacg gcacaggaaa
    14581 aggccggagc aaagcctgta aagtaaataa ctattcagac cccattgtgt gtcgtctgac
    14641 gcacataaaa atagcagatg ttcgttgaat gcttgcattc ataagagcat ctgctatttt
    14701 ttatgtttat ctttcttcta tttttcccga atatggatct tcaggcagct ttcgctcagg
    14761 ggttctccct catgatccag cgtgtattcc accgtcacgt gaatctgatc ctcctgttcc
    14821 tctaccagta ccctgctggt ttcgatcccc agcatcaatt gcccgtaggg tgtgttatac
    14881 gggatcatat gtctcttatt ttcctcgaag atcatatgca tctcagctgt cccctgtctg
    14941 gtcagttcca acagatgatc ccggaatttg atccggctcc tgcagggagc ttggtcttca
    15001 tccattttct cctcatagag cagataatga ctgtcgtttc tcttaaaata ttgtgccggc
    15061 tgcacggtct ccaccgcttc gctttcctgc tcctgtccgg aagcatgcag accggacagt
    15121 acaagttcta cctctcgatt cattttctct tcatcctcta atactcatcg atcttaatgg
    15181 ttttggccga caggattccg tatttaataa tggaattggc aaagtgcttg aacttctccg
    15241 ccccatcgct gacatagaac tggtacttgt tctctcccag atgcggctcc tcatcattga
    15301 gaatatgata atgctgtaac agtcccttca gttctctggc tgtctcgtat gccggattca
    15361 ccagcgtcac ctgatctccc atgacacgtc ccaccgtgga acggatcagc ggataatggg
    15421 tacaccccag aatcagggta tcgatgtcaa tatcaatcaa ctctgacaga taacgctttg
    15481 cgatctcatc agtcacggga tcttcccaca ggccttcttc cactaaaggt acgaacagcg
    15541 ggcaggcctt gccgtagatc gtaacatctt tattcagttc ctgtatgtat ttgttgtaca
    15601 tctggctgcc gatggtagct tcggtggcaa tcacgccgat gcggccgttg cgtgtcacct
    15661 ccgccgcggt tctggctccc ggcttgatta caccgatgat cggaacatcg atctcttttt
    15721 ccagttcatc cagtgcatag gcactcgccg tattacaggc caccacaata gtcttcacct
    15781 gaaaagtctc cagaaaccgc acgatctgct ttgaaaagcg ggtcaccgtc tccttggact
    15841 tactgccgta gggcattctt gccgtatctc cgaaatatat gatcttctca ttgggaatct
    15901 gccgcatgat ctcccgggca acggtcagtc cgccgatacc ggaatcgaat accccgatgg
    15961 gggcatcctt tttcagcgca gtaacattct cctgatacat gggatttctc cttttatttt
    16021 tttaacatcg taagcagtct gctcttgaac cggatctgct ccggatccgc ctccagaatc
    16081 ttccaataga tctggctgtc ggaattccat tctaccactt ccggaccggt ctgtacagtc
    16141 tctgcctccg tggcttccgt ctcctcgtat acgatctcat aggtggacgg cagcatcgta
    16201 agctccgggt agatcacgcc ccaccagccg taacaggcca gtgccacagt cagtatccgg
    16261 actccctgca ccagccggtt taatattttc ttttctgcaa tcatcctgcg atatttttca
    16321 taacaaacat ataatctatc catcagcatt cctctttcca taaattaccg tttcggttct
    16381 atggaaatca ttaccgcttt ccggacagat tatacttttg aaatctattt atttttctcc
    16441 cgttccagac ggatctggcg gatgatcgcc tttttctgat tatctatatg caccttggcc
    16501 atattggatg ccgtttcctt atcctttttc acgatgctct catagatgac cttgtgttcc
    16561 tccaccaggc gctcatgctg gctgaaatcc ttgatatact caaaacgata acggtacatc
    16621 tgctcggaca ggttgttgac cagctggatc aggcgccggt tccccgtggc ctgtacaatg
    16681 atgtcatgca atgccacatc cgcctgtgcg atctgtttaa tgtcatcggt cttcaccgcc
    16741 gcttcaaagg tatcgcaggc ctttttcaga tcctgtaatt cctccggagt gatccggtca
    16801 cacgccagct ccacgcagag cgcatccatg gccctgcgga cctccaggac atcgttcatg
    16861 ctcttctccg tgatctgagc cacttctgct cctcttctgg ggatcatggt caccagtccc
    16921 tccagttcca gcttacggat ggcttcccgg atcggcgtac gactgactcc cagcttgttg
    16981 gcaagatgga tttccatcag tcgttctccc ggcttcagtt cccccgtcag aatggcctga
    17041 cgcaaagtat tgaacaccac atctctgagt ggcagaaatt catccattgt cacctgtaaa
    17101 ttatcctgca tacctctact ctggcattac tcacaaaatt ccgtcaggaa aatctgcttt
    17161 gccagctgtt cctcctctaa tttatcatat gccctgcggg ccatgtcctt ctctacaaaa
    17221 ataccgaata ccgtgggacc gcttccgctc atcagactgt tgatggctcc cagctccttc
    17281 atacgatcct tgatcgtctg gattacagga tatgcactga cggttaccgt ctccagcaca
    17341 ttttccatcc ggtccaggat tccctgtaag ctttcctcag cgatggcttc caccataccg
    17401 tcaatatcag gatgtttcac gatctcctga gcatccagat gttcgtacac gtatttggtg
    17461 gaaacattga tatcgggctt tgccaccagt acatagcagt ccggtgccgg agcaagcggt
    17521 gtcagtttct cgccgattcc ctcagccagc gccgttccgc ccatgacgca atagggaaca
    17581 tccgcaccga tcttcacccc cagttccatc agatccttca gtgtgcaccc aagatcaaac
    17641 agacggttca ttccttttaa ggtagctgct gcatccgtac tgccgccggc catgcccgct
    17701 gctatgggaa tatgcttctc cagatggatc ttcacgcctc cactgatagg ataagtctcc
    17761 atcatcagtt ttgccgcttt gtagatcaga ttattctcat ccgtaggcag ttcaccggaa
    17821 tctgtggtta tcacgatccc accgtctgtt ctctcaaagt ccagcacatc atagattcct
    17881 acagtctgca tgaccatctt cacttcatgg taaccattct ccagtcttct gaccacatcc
    17941 agccccagat tgatcttggc atacgctttt atccgatatt cgttcatttt tgtctccaat
    18001 acgttagggc gctattccaa aactacatgc ctctgaatag cgtgtataca ataattgtac
    18061 ttaattttat acaatcctgc gtattttgtc aatggagtgc ggttctttcc atagattttt
    18121 cccctgtttt tctctaaatc ctctaaagta tccactgtcc ggggacgata taacaggtga
    18181 aaccaaaaac cttcagaagg ttaaagcatg tatagtcaag gatgacaaca ggtacggaca
    18241 gcagaaccgt accggaaaag aggaaactat gagcgtaaac ggagttacat caacccaggc
    18301 cgctgcagcc tacagctata acagcacaag tgcagcaaaa gagaaaactt ccgcagagga
    18361 agcaaaaact acaactaccg cggaagatac cggtgtcatc tatgagcaca gcaccgctgc
    18421 caagacttct tccacgaaga aaacctatac accggatacc aatctggtca acaaattaaa
    18481 ggcagatgcg gaaaaccgtg ccagtcagct tcgttctctg gtagaacaga tgatgggcaa
    18541 gcaggctaac acctacggca atgccaatga catctggtcc tttttacgtt ccggtaattt
    18601 caccgtagat cctgctacca aggcacaggc gcaggcagat atcgccgagg acggttactg
    18661 gggtgtgaac cagacttccg accgtatcat tcagtttgcc aatgctctga ccggcggtga
    18721 cccggacaag atcgagtcca tgcgcgaggc attcaagaag ggctacgcac aggctgagaa
    18781 gacctggggc ggttctcttc ccgagatctc ccagaagact tatgatgctg ttatggagaa
    18841 attcgacaag ctggcagcag atgccggact gaccacagaa gaataatgtc gtattctcga
    18901 cagactttcc tatagaaaaa ccatgttgta ccgatgccac agaacgcatc catgtacaac
    18961 atggtttttc ttttccgaac tgtatcaggc cagacctttt accagcagca ccttctgccc
    19021 cgggcgtaat tcatcgcttt ccagcgcgtt taattctctt acagtcttca ccggcatatg
    19081 atatttcctg ccgatgctcc acaggtcatc ccccggtgcc gcaatataga tcaccgcact
    19141 gggcagtccg gccagcacat tactgtcgat ttcctgctct tccacccctt cgatggcttc
    19201 taacggaata ttcttcatca cggtcgtgga aaaggaaaga acagccttta catccagttc
    19261 ctctccctcc agcagattca cctgcagctg ctccagttca ctgtgtacct ctgcctgatc
    19321 cgttcccgtc actccctgca cattcagggt atattcatac ggaatctgct ctctggtact
    19381 cacataagga gtctcatcat tttccgtaat gcacaacact gtaagatcaa tgctgccctg
    19441 caggcggatt ccttcctctg tggtctcctg atgatcgatc gtcacgattc cctcactgtg
    19501 caacacctgc agtaccggtg agccgatatc ggtctttctg tgatctgtca cttttgtctt
    19561 gcctgtgatt ctggccagca actgacgcag gctggcatct ctggtgtccg ggatcacttc
    19621 ttttgtcacg ccgtaaatat ctcttaaaat atcgatctgc tcttcctcat agagacggat
    19681 attcagttcc agagcacatt ccagcccaag gattctctct tccccgtcca gatccggctg
    19741 gatcgtcagc tctggctgtc cgtgttcctg tcttaccagt gaataccgga tatccggcag
    19801 cagttcctcc cgacatccct gacattccag agtgccattc agcggaattg tcgtctcata
    19861 ggaacgaata gaccgctcct ccccctctgc ctcatagaga acgaagacct gaatatctcc
    19921 ctgcactgcc agtttctctt cctgtggctt gaattccaca tcccgcagtg acacattact
    19981 ccagaggatc tggtaaatat tcggatagct ggagggcagc ggaatctcct cttttttgcg
    20041 aaaaatatcg ttcttgcaga tggaaagctc cgtcacctcc atgggcgaga tccggtattc
    20101 cacaccttct ccggcgctgc ctccctggat tcccacgggc agttcttcat cgtaaatctc
    20161 ttcgatccgg gccgtgagaa gcagcaatgc ctgcagattc agcttccggg aattgatcat
    20221 atttaccgtg aaatcttcta cggttccatt caccgctaca gtatctgacg gcaacgttcc
    20281 ctccatatga attttttctt caaagggcag tgcaccgctt aaaagcacca gattgctccc
    20341 tgtctcctgc gtatgataca gaatggcata acccagtttt ccccgtaccg tcacctcatc
    20401 cgtaccgggc cggatctcct cgatgacaac ttctcccttt tccaggttca gggtactgat
    20461 gtccggttta tcctctggaa gattcagatc ctcctccatg gtaaactgta tgacagtgga
    20521 tcgttttacc cggtccatat gtatatttct cttgatcagc tccacaataa aagacctcca
    20581 tatatacaga tatatctata tatatgaagg tctctgtgta cttataccgt attctgttct
    20641 ctgtatctta ctgcactgca ccgatcagat cggtcagatt ctccacatgg taggtacgca
    20701 tgtaatcctc gatgccctcc actactttca cggtagtata gggatccaca aaattcatgg
    20761 ctccgacgct gacagcgctg gcacctgcca ggataaattc gatggcatcc tccgccgtgg
    20821 caatacctcc cataccgata atggggatct gtaccgcctg tgccacctga tagaccatac
    20881 ggacagcgat gggtttgatg gcagggccgc tcatgccgcc ggtcttgttg gcaatggcga
    20941 attttctgcg gtgaatgtcg atcttcatac cggtgatggt attgatcagg gacagggcat
    21001 ctgcgcctgc tgcctccgct gcctttgcca tctccgtaat gtccgttacg ttagggctca
    21061 gcttcatgat aatgggctgt tttgcatgat ttttcagttc ggaagtaata ttgtacagag
    21121 catctgcttt ctgtccaaac gcaatggctc cttccttgac attggggcag gatacattga
    21181 tctccagcat gctgactgcc ggttcgtctc ccagtcgctc taccacatcc acgtaatcct
    21241 ctacggtctt accgcagacg ttcacaatga tcttcgtatc atattttttc aggaaaggaa
    21301 tatctctctc catgaatacg tcaatgccgg ggttctgcaa accgatggca ttgagcatac
    21361 cgccgtatac ttctgccaca cgaggggtcg ggttgccggg ccagggcaca ttggctacac
    21421 ccttggtcac caccgcaccc aggcggttca gatctacgaa ttcttcatat tccatgccgg
    21481 agccaaaggt tccggatgct gtcattacgg ggtttttgaa agttactcct gcaatcgttg
    21541 tctgtgtatt catcagatct ctacctcctc agcatcaaat acggggccat cggtgcagat
    21601 tctcgcatta tgcacatgag aatgatgatc cacttctttg gtctttacca cgcagcccag
    21661 gcaggcaccc acgccacagg ccatatgctc ttccagagag atataggcct tgatttcttt
    21721 ttcttccgca tattttttga tcgcacggag catgggcatc ggtccgcacg cgaagatcac
    21781 atctgcctgc aatgcatttt cttcgatggc attcatgaca ttgcccttcg tgccgatact
    21841 gccatcctcg gtggcaatat atacggttcc gttctcttcc agatcctctt ttaagaacag
    21901 ctggttatca cggtagccca cgatgatctg cttttctgcg gaagccggaa gctctcttgc
    21961 cagctccagc atgggaggga taccgatacc tccgcccata aggaatactt ttttgccttc
    22021 cgctgcctct gtggggaagc cgttgcctaa ggttcccaga atctccacac tgtctccttc
    22081 tgtataagaa gaaaattccg cggtgccctg tccggcaatc cggtacacaa tgcgcagggc
    22141 atcctttttg tcgcttacct gacagatgct gataggacgg ggcagcaagg tgctccggtc
    22201 cttggggtat acgccgatga actgaccggc ttttgcctgt tcagccagat ctgtagcgat
    22261 ccacatgtca aaaattctgg gtgcgatctc cttctgggaa agcacggttg ctgtgatttt
    22321 ctttttcatg ttattgtcct tttctcttct atttataacg ctatagcgct cttataagtg
    22381 ctctctacca tgggctctac gcttcgtata cgattctgcc tccgcagatg gtcttcctca
    22441 cttcaccctt cagattccat ccggtaaacg gtgtattcgt ggccttggac gcatatttct
    22501 ccggagtgaa ctccgctgcg gtatcgatca gaatcacatc cgccgggccg tcctcggcca
    22561 gatatccggc atccagatgg tacatagccg ccggattggt actcatcaga cgcaacagct
    22621 gcttcatggt cagatatcct ctgtccacca gttccgtaat gcccagcgcc agagacgtct
    22681 ccaacccggt aatgccgctg ggtgcctctg tgatcggctt tgctttttcc tccgcagtat
    22741 ggggtgcatg atccgtagcg atcatatcga tggtgccgtc caccagtccc cggatcaccg
    22801 cctgacggtc cgcttcctcc cgcaggggcg gattcatttt cgccagtgta ccgtacttga
    22861 tcacggcatc ctcagtcagg gtaaaatgat gaggagtggc ttccgcgtgg atattggagc
    22921 agcgcttttt ggcctgacgc accagctcca ccgcttcctt gctgctgata tgctgaatgt
    22981 cgatgcaggc tccggtctct tctgccagct tcaggtcccg ctccaccagc gcgatcaccg
    23041 cctctctggc agaaccgccg ataccgtaat actcgctggc ctttccccgg ttcacaccgt
    23101 tgttctcgat atatttcgga tcttcttcat ggaagctgat gggcatgtcc agctctgcgg
    23161 agatctccat ggcatggcgc accagttccg catccagcag gggcactccg tcatccgtga
    23221 atcccacggc acctgccact gccagcttct tcatatccgt cagctgtttt ccctgcatcc
    23281 ccatggtcac attggcgcag gtggtcacat gaatgcctgt cttcgctccc ttatccagga
    23341 tataatgcag cgtctcctca ctgtccaccg caggcttcgt gttcgccatc agtaccactg
    23401 tggtcacgcc gccttttgct gctgctgctg caccggtgtt gatatcttcc tttgccgtaa
    23461 agccgggatc ccggaaatgc acatgcacat ccaccagccc gggagccacc aataaccctt
    23521 ccgcattgat ccgggtcact ttgcccctcg gttctatctt ttccgcaatc tttacaatct
    23581 tgtcaccgtc gatcaggata tcataattgc cctctctgcc cgacttggga tcgatcatgt
    23641 agccatcatg tatcagtatc atattgtctc cattcccggg cttttaccac ccgccttcgt
    23701 gacctgtttc tcggaaaagt ttatcatgtt ttcatagaaa agaaaagagt gttataatag
    23761 aaaaaaggag gtctgacgac atgaaaacaa gagaggaagc gctgaactac ggattgtctt
    23821 ttccaaatac ttatatggaa atgcccttcc acgatcccaa ctggcagctg gtacgtgtga
    23881 agggcagtaa aaaagctttt ctatggactt atgaaaagga tggctgtctg catctgaatg
    23941 tgaagaccga tcctgcctgg cgggacttct ggcgcagtac cttttcttcc gtcattcccg
    24001 catatcatca gaataaagat cactggaaca ccattatttt agatggttcg atccccgatg
    24061 aggacatccg gcgcatgatc gcagaaagct atgatctcgt caccgacagt cctacgaagc
    24121 ggatctatga agccgtcaaa aagattccga aaggctgcgt cgccacctac ggtcaggttg
    24181 ccgccatggc cggagaaccg aagatggccc gggccgtggg caatgccctg cacaagaacc
    24241 cggatcccga ccatatcccc tgctaccgcg tggtcaattc caaaggggaa ctctccggtg
    24301 cctttgcctt cggcggttcc aacgaacagg ccaaccgcct ccgggccgac ggcatcgagg
    24361 tcaccggcaa taaagtagac ctgcagaaat tcggtatatc gctgtgaaac ttttttcatc
    24421 gcagcctttc gtccatgata ccggaagccg gcaaatttca tcggttctgg ccgatttttt
    24481 accatgtcag caaggccata ctgcggtgtg aaaaaccaat cttctgtccc tgaatgcttc
    24541 catcgagacg acccgtgcct gagaacatag ccgcggattc gccgtcgtag cggaagagat
    24601 ttcctcccag actacttttg tccaggagat gaccggtgct ttacgggacg atgtaaaagc
    24661 actgtaatat ttttcgttgc agttttttta cttttacttc tcaatctggg aacggatatc
    24721 atcctccaga atatgttcca catcttttgc ttctttgcgg atgttttgca gattttcacg
    24781 aatctcccgt tcctcctgta cgatctcggg atgttccttc ttttcttctt taatgaacat
    24841 acacaggaag ctgatcagta atatgatgca ggagatccag atggtcctcg cccctatgta
    24901 gccgatcata tgtccgccca gcgccgcacc aacggcaaac aggaaaatga ttccaaaata
    24961 attgccggat ttccgtaaga tttttttgtc cttcgtcttc ccataggcac acagggattc
    25021 catgccgctg cgcagattac cgatacacat ggtactggca aaagcataac cgttcacctt
    25081 gcggaaagcc tgtacctgca tggcgcaggc gaaagatacc atggcattgg ccagcagatc
    25141 catctcattc gggaaaaatc ccacagcaaa cagcaacaga atctctatag caagcaccag
    25201 ctgccgccag tggatcctct gagccttctg atatcgcata tggatgcact ccgctgccgc
    25261 tactcccaac gcgaagaaca aaagcgggga aaaataccga agcgccacgg accagttccg
    25321 gtctaccaga ttctgggcca gcaacacgat attaccggtc tgggcattgg cgaatacctt
    25381 gccacggtat atataagtat aagcatcctg taatccgccg gaaattgaca aaattccagc
    25441 aatcagaaac gactctgaca tttgtccgtg taatttacgt tgcatagtaa ttctcattcc
    25501 tttcagtaac ctgcagattc tgtctccgat acatacaatt accttcctac aggcatctcc
    25561 tgattacagt acaggcaacg atatcttccg gaaggtgaca gttcaaatat gtggtcacaa
    25621 ttcgtctcta tactggtaat acaacgggga tttttacatt ttatcacatt aaccagtctt
    25681 tgtggaggaa ccggatgata cttttttgca acttttccgt cccggatcac gatcagtgtg
    25741 atctgactgt ccagatatcc cagaatatcc aaccgctccg gcaacgtctc gccctcaatc
    25801 ttgatcagat ccttgcagcc gtatttctgg cttttcaccg attgcagcaa tgctacagac
    25861 tgcgtcttgg attccaattc cagcaatctg tagatgtcca ttccctttcc cgcgggaata
    25921 tgatcgatca caacaccatt ctgaatggat tctacgttta acataaaaac accttctttc
    25981 ccgtcacagg atccgtaagg tctaacagac ttaagatcaa tgccatacgg ataaatttac
    26041 cattcaatac ctgctgaaaa tacgctgctc tcggatctgc atccacttcc ggtgcgatct
    26101 cgtccactct tgggagcgga tgaagaaccg gcatgtccgc tttcgccaga ttcagtttct
    26161 cctgatccaa aatgtagata tcttttaacc ggatataatc ttcctcattg aaaaaacgtt
    26221 ccttctgaac tctggtcatg taaagcacat ccagctctgg cagtgtctcc tccaggctgg
    26281 atacctcatg gtagggggct ttggccggtt tcagagtatc ctcgatcaga tattccggaa
    26341 ccttcagttc ctccggagaa atgaaataaa attcattgcc cggatagcgc accagactgt
    26401 tcaccagaga gtgtacggtt ctgccgaatt tcaagtcacc gcaaaagcca atcttcagat
    26461 gatccaagtg tcctttgcgc tgtcttatgg tcagcagatc gatcatggtc tgggtcgggt
    26521 ggttatgtcc tccgtccccg gcattgatca ccggaatccg ggaatacatg gaagctcgga
    26581 gcggtgctcc ctctttcgga tgccgcatgg caatgatatc tgcatagcag ctgaccaccc
    26641 gtgcggtatc tgccaccgtc tcacctttcg tggcactgca ctgttccgca ccggcaaatc
    26701 ccaatgttct gccgccaagg ttcagcatag ccgtctcaaa agataatctg gtacgtgtgc
    26761 tgggctcgta aaataaggtt gccagtattt ttccatctgc acaatgagca tatgcagcgg
    26821 aatcggcagc aatccggtct gccaggtcta agatttcctg cgtctccgct accgacaggt
    26881 ccagtgaatc aatcaaatgt cgcatatcct tttctcctct gtttactttt tttcaaccgc
    26941 gtattatact atatccacac ggatactaaa aatatatagt ttatatgcga ggtatatcat
    27001 ttttatatgg atattagttt tgaatattac aagattttct attatgtcgc aaaatacgaa
    27061 aatatcacca aggctgccat ggtcttaaaa agcagccagc ctaatgtgac ccggattatt
    27121 catattctgg aggatcagct ggactgccgt ttatttctcc gggaaccccg gggactgaag
    27181 ctgaccgaag agggaaaacg gctctatgcc catgttgcca tcgcctgtca acatctgttg
    27241 gatgcggaag ccgaactgtg ccggaacaaa aacgtgtgca gcggcaccat tgaactgggt
    27301 gtcaccgaga ccgccctgca tttatttctg ttacagaatc tccatgattt ccagactggc
    27361 tatccggaga tcaaaatacg tatccagaac aataccactc cggagattct gaaatctctg
    27421 cagaacggcc ggctggatct ggctgttgtg acaacccctt ttgaacttca tgatgctctg
    27481 gcctgcgaag atctgctgac tttccgggag gttcccgccg gcggccccga ctatgccgcc
    27541 ttgtctgatc gtcccatgac gctgaaagaa ttccggctcc attccatcat tggtctggga
    27601 tacggcaccg ccagctatga atattaccgg aaggtattta ttgaacagca tctggactac
    27661 gaacccgcca tggaagtagc taccgccgat ctggtcatcc ctctgctcca gaacaatctg
    27721 ggagtgggct tcgtgccgga gcctctggca gctccctttt ttgaaaacga cagtctcgtt
    27781 ccgctgacct taaatgtgcc tctaccgtcc cgcgaagtaa aaatgctgtg ggacaaaagc
    27841 cgcagtcaga gccgggctac cgctactttc tgcagttatc tgcgggaacg ctgtcagcgt
    27901 aatctgccaa tcctggatcc tgaaaatgag tctgttgctg gcaactaaat catatttcag
    27961 gaggaaaaaa aattgaaaaa tccatttaaa aattttacaa aaaaggaatg gatcctgtgg
    28021 atcgcatccc ttttcattgt cataatatca aatctgctga ccgctgattt tgacccgtta
    28081 actttgctgg ctgcgctggt gtgcgtcact tccctgatct ttgccgccaa tgatatgtat
    28141 ggttttatca gctggcggcg ccgggagatt ttgatggatc tgtaagaacc attcgtttaa
    28201 tcataaagca cggttttatc cagaagttct gtttttaaca caatgtaagg cgtggagggt
    28261 ttccctgcgc cttccttttg tccggtgatg tccgggccta agaggctggt gctgacatga
    28321 atagcattgt ccgtcagata cagggcatcc acagcaatgc tgtacccacc ggtcttctgc
    28381 tcaccgtaac caatactgat atagaggttc tgattatcac tgtaggtcag ctgaaatggc
    28441 tccgttcctt tttcagcgat tacagtcttt aattcctcgg gaattttttc ttcactgagt
    28501 accgtaaaat ccagatctct gagtttcaca ttctctttcg aaagcagacc gcagcctgtg
    28561 agcagcaatg cccccagtac tgcggtcatt atgattttcc actgtctcca tttgattttc
    28621 atccgtcatc caccgttaaa acttccttat tgcatattta tgtgaaaaaa gcagaaatat
    28681 actcctgttc tgtacctatc tcattaattt tcatcctttt ctcctttgaa aatgtgcgtt
    28741 ataataggtg atgtgcgtca aaagacgcct tacagaaaga tgagggaatc ataattatgt
    28801 atgataaatt cagcaggaaa gtcttctgcg acggtatccg cgacggcttt cccattgctc
    28861 tgggatattt cgcagtatca ttttctttgg gaattgccgc aagacatgca ggctttaccc
    28921 ccatgcaggg ttttctggca agcctgttaa ataacgcttc cgccggagaa tatgcagcct
    28981 ttaccctgat tgcggcacag gccacatatc tggaagtggc gatcatcacg ctgattgcca
    29041 atgccagata tctactcatg agttgtgcgc tggcccagaa attttctccc agcacgccct
    29101 ttttccaccg tctgatcatt gcttacgatg tcaccgatga gttgttcggc attaccattg
    29161 cccggccggg tatcctgaat ccgtactata cctacggtgc gatcctgttg gcagcgcccg
    29221 cctgggccgg aggaaccgcc tgcggtatca tcgtcggaaa tgcgctgcct cttcgtgctg
    29281 tcagcgccct gagtgttgcg ttatacggaa tgtttctggc cattatcatt ccgcctgcca
    29341 gaaaaagtaa gattgtggca gctctggtag caatcagctt cgcactgagc tttgcctgca
    29401 actatctgcc tgggatttct tccctgtcag aaggtacgag aaccatcctg ttgaccgtga
    29461 tcatctccgg tgctgcagca atcctgttcc cggtgaagca gggtgcagag gaagccgttc
    29521 ctgcggaaaa cggagaatct gtacagaaag gagaccacgt aaatgaataa ttatatttat
    29581 attgctgtta tggcggctgt ctcctatacg atccgtgtct tacccctgac actgatccgt
    29641 aaacccatca agaatcagtt tattcagtcc tttttgtact atgtacctta cgtgacgctg
    29701 gccgtcatga ccttcccggc catcatccat gctacccaga cccccatttc cggactggtg
    29761 gcactcattg ccggtatcat cgccgcatgg ctcggtgccg gactgtttcc ggtatccgtg
    29821 atctgctgtg tgctggtgtt tgtgatcgaa ttgtttgtat aatgccttgt aataaaacac
    29881 gccattgaga aggattcctt ccccatggcg tatttttatg gtccggcttc ttcacagaag
    29941 cttttacaaa tcatattttt ttaacatttc ctgtacgaat tccggggttc tttgtcatgg
    30001 tttccaacat cttcagatca tccaccgttt ctacattgaa caattgtatc cactcatcca
    30061 tccgctgccc ggtcaatgct ttgttcaatt ccaataatac atctacgatt ccctgcttct
    30121 ggtatttaaa acaaatgttt tgcgcaatag ccctcgaaat tattgtgcac tttttgttac
    30181 atttttatct ttgatggatg ataatatgct ataatatata tgcgcaagcg gttgttcgat
    30241 tatgttttgt gggaggatta cttatgcaac attttagcaa gcaattacgt acacggctgt
    30301 tgactctgta tctcagtgcg ggactggtca tgggctggct gcccggatgc ggcagtcagg
    30361 atctggaaat cgcagataca gtggcagaat ccgcagttac cgctactgca gatgccacgg
    30421 atgtcatcac tttttcccag ccggaagggc cggaggagtc taccgtttat gtggagccgg
    30481 tggacggcat ttccgatgat ttttaccgtg gtatggacgt atccgcagta ttggctctgg
    30541 aaaacagtgg cgtgaaatat tataactttg acggagaaga gcaggatgta tttatgacgc
    30601 tggcgcaggc tggtgtgaac tatatccgtc tccgggtctg gaacgatccc tatgatgaga
    30661 acggcaacgg ttacggtggc ggcaacaatg acgtgacgac tgccatcact ctgggacaac
    30721 gtgccacgca gtatggcatg aaggtctgca ttgatttcca ttattccgat ttctgggcag
    30781 atcccaagaa gcagttcgtt ccaaaggcct gggaaggtat ggatatcgaa gaaaaatccg
    30841 acgctctcta taacttcact ctggaaaacc tgacgcagat tctggatgcc ggtgtggacg
    30901 tcggtatggt acaggtgggc aacgagatca acaacggcat gtgtggagag acagatgcat
    30961 caaacgttcg aaaactgttg gcctccggca gtaaggctgt cagggatgct gccactgctt
    31021 ccggaaaaga tattctggtg gctgtacatt acaccaacat cgatgatatg aaaaagctgg
    31081 atacactgct gaccggtctg caggtaaaag aaatcgatta tgatattgta ggtctttcct
    31141 tctatcccta ctggcacggc acgatggacg atctgaaaaa tgccattatc catatccgtg
    31201 atacttatgg caaaaaggta tatgtagcgg aaaacgctta ctgttatact tcggaagacg
    31261 gcgatggctc tgccaacagt atcaagggaa ccgatgatct ggcagaagga tattcagcca
    31321 gcgtacaggg gcaggccaac gaagtgcggg atgtctgtgc ggctgccagc gaggccggtg
    31381 ccgaaggcat cttctactgg gaaggtacct ggatcccggt cggtcccgcc gatgctgata
    31441 attctgccat ctgggaaaaa tacggcagcg gttgggccag cagttatgca ggcggttatg
    31501 atccaaagga tgccggacag tattacggcg gaagctcctg ggacaatcag gccatgttcg
    31561 acttcaccgg acatcctctg gcctccctaa atatatttaa atatctgaag tacggagcca
    31621 ctgctcctct ggcgatagac accatccccg aaatagtggt gtcctgcaat atcggtgcgg
    31681 aagtcaagct tccggatacc gtaagtatca tctataacga tcgttccgaa gaacaggttc
    31741 ctgtcacctg ggatgctgaa gatattgccg ccatcgacac ggaaaacggc ggagagttca
    31801 cagttgccgg atctctggat gaaggtacgg aagttactgc cattgtcacg gtagaacggg
    31861 tcaattatgt acagaatccc ggctttgagg atgctgatac ttctatgtgg actgtaacat
    31921 actccggtga aacgaatccc accgactatc aggtcaaagc tgccgatgcc cacagcggtg
    31981 aggtggcttt ccacttctgg tccggtacct ccgatatgga cttttccatt gagcagagct
    32041 tcacagatct ggaacccggt acttatgaat tgtccgcttt ctcacagggc ggtgacctgg
    32101 cagcggatgc ttccatggag ctgtatgctg tcgtagacgg caaggaactg acagtgcctt
    32161 ttatgctcac cacctatgcc gactggcaga atcccaccgt ctccggtatc aaagtcaccg
    32221 acggcaccct gaccattggc gtgagatatc aatgtaataa gaacagctgg ggaactctgg
    32281 atgatgtgac attgcatcgg gtcgggaatt aaatagaaag ttagtgaaat acattgacaa
    32341 aatcaacgaa ctcccggagc atgcgataca catgtctcga tgtaaggtct ccggatgtga
//
LOCUS       DAWDHD010000009        32091 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_116_length_32091_cov_13.019572, whole genome shotgun sequence.
ACCESSION   DAWDHD010000009 DAWDHD010000000
VERSION     DAWDHD010000009.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 32091)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 32091)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..32091
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116_length_32091_cov_13.019572"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2169..2268
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         7676 a         8898 c         6441 g         8976 t
ORIGIN      
        1 acttcacgct tcttcttggt atcacggatc tccgccttac cgccgaactc agcgatgatc
       61 gcaagtccct tcggctttct ggcctcgaac agctcctcga cacggggaag accctgagtg
      121 atatcgtcac ctgcgacacc accactgtgg aaggttctca tggtcagctg ggtaccgggc
      181 tcaccgatag actgtgcagc gatgatacca actgcttcac ctacctgtac ggcttcaccg
      241 gttgccatgt tggcaccgta acattttgca cagataccaa cgtgtgaacg gcaggtcagg
      301 atcgtacgga tcttcacttc ctcaatgggc tcaccctgtg cattgacacc aacacttaag
      361 atcttggcag cacggctggg ggtgatcatg tggttgtgct taacgatcat attgccgtct
      421 ttatcataaa tatcctcgca ggaatatctt cctgtgattc tgtctttcag gccttcaatg
      481 gtctctttgc catccatgaa tgccttgact accataccag ggatctcagc ctgaccctca
      541 cagcagtcta cttcacggat agaaagttcc tgagatacgt caaccattcg tctggtcaga
      601 taaccggagt ctgcggtacg cagagcggta tcggacatac cttttcgtgc accatgtgcg
      661 gacatgaagt attccagtac gtccagacct tcacggaagt ttgacttaat gggcagctcg
      721 atggttctac ctgtagtatc agccatcagt ccacgcatac ctgccagctg tttgatctgc
      781 tgactggaac cacgcgcacc ggagtctgcc atcatgaaaa tgttgttgta tttatccaga
      841 ccggacagca gtacgtcggt cagctcttta tcggtctcgt tccaggtctc tacaacggca
      901 cgataacgct cttcctcggt gatcagacca cgtctgaagt tgcttgcgat cttgtctacg
      961 gtagcctgtg cagctgccag catttcaccc ttcttctcag ggacggtcat atcggaaatg
     1021 gatacggtca tagcagctct ggtagagtac ttgtaaccga tagccttaac atcatccagt
     1081 acctctgcgg tcttggatgc tccgtgggta ttgatgacct tctccaggat ctgcttcaac
     1141 tgcttcttgc ctacgtggaa gtctacttcc agcttcagga agttctcggg atcgcttctg
     1201 tctacgaatc ccagatcctg aggcaggatc tcattgaaga tgaaacgtcc cagagtggac
     1261 tctaccgtac cggtaatggt ctcgccctct gcatttacct tgtgcatacg cacatggatg
     1321 cgggaatgta aggtcagcgc ctggttctca tatgccagga ttgcttcgtt tacgctcttg
     1381 aaatacttac cttcaccgat cgcaccgggt ctctcctggg tcagatagta gataccaagt
     1441 accatatcct gggaaggaac ggctacgggt ccaccatcgg aaggcttcag taagttgttg
     1501 ggagacagca gcaggaaacg acactctgcc tgtgcctcct gggacagagg aagatgtaca
     1561 gccatctggt ctccatcgaa gtccgcgttg aatgcggtac ataccagggg atgcagcttg
     1621 attgccttac cttctaccag gatcggctca aatgcctgaa tgcccagtct gtgcagtgtc
     1681 ggcgcacggt tcagcataac aggatgttcc ttgataacct cttccagcac atcccatacc
     1741 tgtgtatcca gcttttctac cagcttcttc gcattcttga tgttctggct gattcccttg
     1801 gctaccagtt ctttcattac gaaaggcttg aacagttcga ttgccatttc cttcggcaga
     1861 ccacactgat agatcttcag ttccggtcca acgacgataa cggaacgtcc ggagtaatct
     1921 acacgcttac ctaacaggtt ctgacggaaa cgaccggtct taccctttaa catatcggac
     1981 agagacttca gtgctctgtt accggggccg gtaacaggac gtccgcgtct gccgttatcg
     2041 atcagagcat ctaccgcttc ctgcaacatt ctcttctcgt tacgaacaat gatatcagga
     2101 gcaccaagct ctaacagtct cttcagacgg ttattacggt tgatgatcct tctgtacaga
     2161 tcattcagnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnac agtctcttca
     2281 gacggttatt acggttgatg atccttctgt acagatcatt caggtcggag gttgcaaaac
     2341 gtccgccatc caactgtacc ataggacgaa gatcgggagg gataacagga ataacatcca
     2401 tgatcatcca ttcgggctta tttccggact cacggaaagc ttccacaact tccagtctct
     2461 taatgatgcg ggcacgcttc tgaccggagg attccttcag tccggtcttc agttctgctg
     2521 catccttctc caggtcaatg gcctgcagca actctttgat agcttccgca cccatgccta
     2581 cacggaactt gttgccccag gtctctcttg catcctgata ttccttctcg gacagcactg
     2641 ccttgtattc aaggttcgtg gttcccggat ccagaacgat ataggaagcg aagtacagaa
     2701 ccttctccag gattctggga gacagatcca ggatcaggcc catacggctg gggatacctt
     2761 tgaaatacca gatatgggat acgggagctg caagctcgat atgtcccata cgctctctac
     2821 gtacgctggc tttggtaact tctacgccac aacggtcgca gacaacgccc ttatatctga
     2881 tctttttata tttaccgcaa tgacattccc agtctttgct gggtccgaag attctctcac
     2941 agaacagacc gtctctttca ggcttcagtg ttctatagtt gatcgtctcg ggctttaata
     3001 cctcaccatg ggaccactcc cggattttct ccggggaagc taatccaatc ttgatggcat
     3061 caaatgtcat aggctgatag gttgtttcat tttttgtttc tgacattaat ggcactccct
     3121 tctatacaaa gcattaaaat tcatcatcac tgccgtcaaa gctatcatcg gtgtaatcat
     3181 cgtcaacgta atcgtcgtcc tcagagttca ccagctcacc attgtcatca aattcctgtg
     3241 actgatagcc catctcgcca agggagctct tgtcgtaatc gtcatcgtac ttacgatcac
     3301 cttccatctc ataacggtaa tcggtatcgc cgtaatcgac agtctccatg atctcaacct
     3361 cggtctgatc ctcacgcagc actctgacat ccagacccag tgcctgcagt tcctttaaca
     3421 gaaccttgaa ggactcgggg ataccgggct cagggatatt gtctcccttg atgattgctt
     3481 cgtaagtctt cacacgtccg atcacatcat cggacttcac agtcagaatc tcctgcaggg
     3541 tgtaggatgc gccatacgcc tccagagccc atacttccat ctctccgaaa cgctggccgc
     3601 cgaactgggc tttaccaccc agaggctgct gtgttaccag ggagtacggt ccggtagaac
     3661 gcgcatggat cttatcatct accagatgat gcagtttcag gtaatgcatg tgtccgatcg
     3721 ttacagggcc atcaaagtat tcaccggttc taccgtcacg aagacgtacc ttaccgtctc
     3781 tggaaatggg aacacccttc catactcctc tgtgatctct gttctcagac agatactgca
     3841 taacctcagg aagaagctct tcaccatgct tgctggtaaa ggtctctcct gcccagggct
     3901 taccgtctgc ggtagtttcc tgtccctttt ctttccactc tgcactttct gcatcgtcga
     3961 aaggaaggtt tacataatcg ttggcaagat ccaacgtatc cataatatct ttctcgttcg
     4021 caccatcgaa gacaggtgtt gccacattga atcccagagc cttggcagcc agggaaaggt
     4081 ggatctccag gacctgtccg atattcatac gggaaggcac gcccaggggg ttcagcacga
     4141 tgtccaaagg acgtccgttg ggcagataag gcatatcttc tacaggaagc acgcgggaaa
     4201 cgacaccctt gttaccatga cgacctgcca tcttgtcgcc tacggagatt tttctcttct
     4261 gtgcgatata aatgcggact gcctgattta ctccgggtgc cagctcatcg ctgttctcac
     4321 gggtaaatac cttggcatcc actacaatac catattcgcc atgaggtacc tttaaggagg
     4381 tatctcttac ttctcttgcc ttctcaccga agatggcacg cagcagtctc tcttctgcgg
     4441 tcagttcagt ctctccctta ggagtaacct tacctaccag aatatcaccg gcacgaacct
     4501 ctgcgccgat acgaatgata cctctgtcat ccagatcctt cagtgcatcg tcaccgacac
     4561 cgggaacatc acgggtgatc tcttccggtc ccagcttggt gtcacgggct tctgcctcat
     4621 actcctcgat atgaacggaa gtataaacat cgtcacgtac cagtctctca cttaacagaa
     4681 ccgcatcctc gtagttgtaa ccttcccagg tcatgaaacc gatcagaggg ttcttaccca
     4741 gtgccagctc accctgagag gtggaaggac cgtctgcaat gacctgacct gccgctacat
     4801 ggtcaccctt gaatacgata ggcttctggt tgtagcagtt ggactggtta ctgcgggaga
     4861 acttggtcag atggatctct gccttttctc cgtcatcata agcaatgcga atgatcttgg
     4921 aagatacata ggtaatggtt ccgggcttct tggccactac gcagacaccg gagtcaaccg
     4981 ctgtctttac ttccataccg gtaccaacca caggagcctc agtgcttaac agaggcacag
     5041 cctgacgctg catgttggat cccatcagcg cacggttcgc atcatcgttc tgcaggaaag
     5101 gaatcagtgc agtagccaca gagaatacca ttctcgggga tacatccatg taatcaaaca
     5161 tttccttagg atattcctgt gtctcatcca gataacgtcc ggaaacagat tttctcacga
     5221 aatgtccctc tgcatccagt gcttcgtttg cctgtgccac atggtaatta tcctcttcgt
     5281 ccgcagtcat gtagactact tcatcggtaa cgcgggggtt cttaggatcg gacttgtcga
     5341 tcttgcgata aggagcctct acgaaaccat attcattaat tcttgcatag cttgcaagag
     5401 agttgatcag accgatgtta ggaccttcgg gtgtctcgat agggcacatt cttccataat
     5461 gggagtaatg tacatctcga acctcgaatc cggcacggtc tctggacaga ccgccggggc
     5521 ccagtgcaga cagacgtctc ttgtgggtca gctcacccag agggttattc tgatccatga
     5581 actgagacag ctgggaagat ccgaagaact ccttcactgc agcggttaca ggcttgatat
     5641 tgatcagaga ctggggagaa atatcctcag catcatgtgt tgtcatacgc tcacgtacca
     5701 ctctctccat tctggacaga ccgatacgat actggttctg cagcagctca cctactgcgc
     5761 ggatacgtct gttgcccaga tggtcgatat catcatcgtt gcccaggcca tactccagat
     5821 gcatattata gttgatggaa gccaggatat cttccttggt aatatgcttg ggtaccagtt
     5881 catgtacgtt cttcttgatt gcttctgcca gcttctcggg atcatcgccg aactcttcca
     5941 ggatctgtgc cagaaccgga tagtatacca gttcatggat accgaaatcc ttgggatcgc
     6001 aatctacata gtgggtcaga tctaccatca gattagacag tacttttaca gttctctctt
     6061 cggtctggat atatacgaag ggaactgcgc tgttctggat ggtatctgcc agctctgcgg
     6121 tcaccttgtc tcctgcggat gcgatcactt caccggtgga aggatccact acatctgcag
     6181 ccaggatctg atgacggata cgattcttca gagctaactt cttattgaat ttatatctgc
     6241 cgactttggc taaatcatat cttctggggt cgaagaacat agcattgatc aggctctcag
     6301 cactctcaac gcttaaaggc tcaccgggac ggatcttttt gtataactct aacagaccct
     6361 cctgataatt ctcagcagtg tccttggtaa agcttgcctc gatcttgggc tcgtcaccga
     6421 agagttcacg gatctcatca ttggtaccga tacccagggc tctgatcagt acggtaatgg
     6481 gaaccttacg ggttctgtcc acacgtacat agaagacatc gttagagtcg gtctcatact
     6541 ccagccatgc accacggttc gggatcacgg tacaggagaa gagcttctta ccgatcttat
     6601 catgtccgat tccgtagtag ataccgggag aacgaaccaa ctggctgacg ataacacgtt
     6661 ctgcgccatt gattacgaaa gtaccggtct cagtcatcag cggaagatca cccatgaaga
     6721 tctcatgctc actgatttct tccttctcct tgttataaag gcgaacctta accttaagag
     6781 gtgctgcaaa ggtagcatct ctctctttac actcttcgat agaatacttc acgtcgtccc
     6841 tgcagagttc aaaatccaca aattccagac tcaaggaacc gctgtagtca gcgatcggag
     6901 agatgtcgtc aaagacttcc ttcaagcctt cgtctaaaaa ccactgatag gagtccttct
     6961 ggacctcgat cagattgggc atctctaaaa cctcttcttg tcttgaataa ctcatacgca
     7021 taaccttggt accggcaatc ggacgtattc tgttcttttc cattgacatt tcaccccgtt
     7081 ctttcagatt taaagcctat tttgcgtcgg caacaatgca gtatatataa acgccagttt
     7141 tttggcatga caaataagca tggtaccgca caatagtgca ctatccataa tagcacaatg
     7201 ttagaaatac gtcaaggaaa tattttgaat tatttaaaaa gaatagatat ttttaaaaat
     7261 taataactga cagatgcgaa taagaaaaac catgcaccgg caggtgccgg cacatggtcc
     7321 gtcatatact ggttaaaaat tacttcagag taacctttgc gccaacttcc tcaagcttct
     7381 tcttgatgtc ctcagcctct tccttagaag cgccttcctt aacaacctta ggagctccgt
     7441 caactacgtc cttagcttcc ttcaggccca gaccggttac ttcacgaaca accttgataa
     7501 ccttaacctt ctcagcgcca acctcggtca gctctacgtc gaactcagtc ttctcttctg
     7561 ctgcttcagc agggccagct gctgctacaa caacgccagc tgctgcagat acgccgaact
     7621 cctcctcgca agcctttacg agatcattta actctaatac agataactct ttgatagcat
     7681 cgattaattc ctgtgctgtt aatttagcca ttttaatttc ctccatttga aatattttga
     7741 ttgattgtca attagctgca attattattc agctgtgggt gctgcttcgc aagctgctgc
     7801 gccgcctttt tcagccagct gattcataac acgtgcaaaa ttggtgatag gactctgtaa
     7861 gctgccaagc agtttggaaa tgagtacgtc tctggaaggg atgttagcga ttgcagccat
     7921 acccttagca tcataaacag taccttctac cacaccggcc ttgatctcaa gcttatcagc
     7981 agtcttagcg aactgagcca gtactcttgc aggagcggtt gcatcctcgg tagagtaagc
     8041 cattgcgctg gggccttcca gatactcgga cagaccttcg aactgtgtac ccttgaacgc
     8101 aaagttcatc atagtgttct tgtaaacctt gtagctaact cctgcctcac gtaagctctt
     8161 acgtaactgg gtatcctgct caacagtaag tccacggtag tctaccagaa ctacagactg
     8221 ggcatctttg ataccagcag aaatctcttc tactacaggc tgttttaatt caacttttgc
     8281 catttcttta cctccttata gattttgtgc cgataagaag aatataaata gaatcctgct
     8341 cgcaggattc tacgcctgcg gcgaatcgct ttagcgatat attccacttc gccgcagtgc
     8401 gatcctcgcg gagcgttttg ctttatagga taaaatccta taaagtaaaa aaaactctct
     8461 gtgaagacac agagagatga tatcatcata cttatctcct cggcaggact tacgctcggt
     8521 ctcccttgca cctgctgtct tcggcatggt tttacaacct cagatagaat agcatgggag
     8581 accgtgtttg tcaaggatta tttaataatt ttctgcatga atttcaaaat agctctgggg
     8641 atgattacat acggggcaaa cctccggtgc cttggtgcct accacgatat gcccgcagtt
     8701 gcggcactcc cataccttca cttcactctt ctcgaacact tttgctgtct ccacgttctt
     8761 taacagttcg cggtatcttt cctcatgatg cttttcgatc tctccaacca tacggaattt
     8821 ctgcgccagt tcagggaatc cctcattctc tgcagttgcc gcaaaatctt catacatatc
     8881 agtccactcg aaattctcac cttcggatgc tgcagcaaga tttgctttgg tatcaccgat
     8941 gccgttcagt tccttgaacc acatttttgc atgttctttc tcattatctg cagtctttaa
     9001 gaaaatagct gcgatctgct catacccttc cttctttgct acagatgaaa aataagtata
     9061 tttgttacgt gcctgggact ctccggcaaa tgccgcctgg agatttttct ctgtctgggt
     9121 tccctgatat ttgttcatgg tgttttctcc ttctcttttc atctactata ccctttggag
     9181 gtaaccttta ttctaaaaca tacggtttct ccgttcttcg aacgttcgaa ttcgtaacca
     9241 gcatacctgc aggcagccag ttattgtttc ctaaggataa atgtattcta tcacaaaaac
     9301 cgcctcactg taaacagtga agcggctctt ttgcattcgt tatctaatta gattagagat
     9361 cagattagaa ctttgctacg ctaaccttaa cgccaggtcc catagtgctg gtcagagtga
     9421 tgcttctcag atactgaccc tttaatgcgc taggcttagc ctttacgatt gcgtccataa
     9481 gtgcattgaa gttctcctgc agagcttcct cagagaaaga agccttacca acgggaacat
     9541 gcacgatgtt ggtcttatca agtctgtatt caatcttacc ggctttgata tcgttaactg
     9601 ccttggttac atccatggtt acggtaccag ccttagggtt aggcattaag cccttaggtc
     9661 cgagaacctt acccagacga cctacaacac ccatcatatc aggggttgct actacaacgt
     9721 cgaagtccag ccatccttcg ttctggatct tcgggatcag ctcctcgcca ccaacatagt
     9781 cagcgcctgc agcctcagcc tcattgatct tatcgccctt tgcgaatacc agaactttta
     9841 cagtcttacc agttccgtta ggcagtacaa ctgcgccacg gatctgctgc tcagcgtgac
     9901 gtccgtcaca accggttctg atgtgaactt cgatggtctc atcgaattta gcagttgcag
     9961 ttttcttaac cagagcgata gcatcgttgg gctcatagaa tgtagcacga tctaccagct
    10021 tggcagcttc ggtatatttc ttaccatgtt tcattatttt acctcctagg ttccagcggc
    10081 aatattttat tcattgcctc ccaaatattt ataagttact cctcaacaac aatacccatg
    10141 ctgcgtgcag taccagcgat catgctcata gctgcttcca ggcttgctgc attcagatcc
    10201 ggcatcttca tctcagcgat ttcctgaacc ttagccttgg aaatggtagc taccttggtc
    10261 ttgttgggaa cgccggaacc ggacttgatg ttgcaggctt tctttaacag tactgctgca
    10321 gggggagtct ttgtaacgaa actaaagctt ctgtctgcat atacagtaat aactacgggg
    10381 atgataagat ctcccttgtc tgctgttctt gcgttgaact gcttggtaaa ttcaacgatg
    10441 ttaaccccgt gctgtccaag tgcaggacca acagggggag ccggtgttgc tttgccggca
    10501 ggaatctgta acttgatata tccttctact ttctttgcca ttgtggcacc tcctataatg
    10561 tggtaattcg gcgcaggctg ccttgttgct cgcctcctgc tcccactgga acgacttctc
    10621 gttcctttca tgctgccact ctcttcacct tgcggtttct gagagtttta tattttatct
    10681 ccggaattgt ccgtgagaaa aaatatcctt gtaagcttat taaagctttc tgacctctgc
    10741 aaagctgatt tccacagggg tctctcttcc aaacatttct acattgatcg tagctgtctg
    10801 tttgccatag tccagtttct gcaccacgcc gacggtatcc ttccatactc cggccacaac
    10861 agcaatgctg tcgccttcac caaagtcgat ggatacattc tcggtcttaa tgcccaaagg
    10921 cttcatctca gcttctgtaa gaggtacagg tttacttccg ggacctacga aaccggtcac
    10981 accacgggta ttacgaacga cataccaggt atcatcgttc atctccatgt tgagaagcac
    11041 ataaccgggg aacattttct tggagaccgt cttgcgggcg ccgtctttca gttctacgac
    11101 atcctgcatg ggaactctta cttcaaggat ctgttctgcc agactcttgt tgttctctat
    11161 ggtcttctca atattggctt tgaccttgtt ctcatatcct gaataggtat ggactacata
    11221 ccattttgcc tctgccatac aatcaccctc gctctataat gatgttaaga agtttacgcc
    11281 gtactggata aaataatcca gaaccgcaat aatcacaccc actacaactg atattgcaac
    11341 aaccgctacg gattgcttca atgttgattg tctgtccggc catgaaattt tcttaaattc
    11401 agcttttgcg cctttaaaga aactggggcg cttctgtttt tctgcggaat ctcccatttc
    11461 gacctcccat actagaacgg ctgattattt ggtttctttg tgcaatgtat gtgtcttgca
    11521 gaatctacaa tacttcttgg tttccattct atcaggatgg gtcttcttat ccttggtagt
    11581 attgtagtta cgctgcttgc actcggtaca tgctaaagta atttttgtac gcatttttct
    11641 acctccacgt gttttctgat ctctaaattt aagcataaaa aaaagaccta aatctcatct
    11701 cgcttggata ctataacata atctgcggtc ttcgtcaact gttttcccaa aaatcccact
    11761 atttttcttg tttcttctta tttattatgc tataatgatc gcataaaagt ggggtattgg
    11821 atttccccgt cccaattcat ccgcagggaa gaaaggagat gcctatgagt gctatcatga
    11881 acactgtcta taataactac ctgaccacct atgcatcaag acagattaca aaatatgaca
    11941 cccataaaaa gagtgagctt cgcagtgtat acaactctat cgtaaagctg aacaaggaca
    12001 ctccctggta tcttcccacc accaataaag acacacagca ttatgccgtg gatctgaagg
    12061 aaaacgcccg cgaactgcac aacaccatcg cacagatcgg cggattggag acggatggac
    12121 tgttccggaa aaagagtgcc tactccacag acgatgatat cgtggaggct tcctacatag
    12181 gctccgatac caccacagga gcttctccgg aatttgatat cgaagtaaaa cagctggcca
    12241 tgccgcagga gaatctgggt tattttcttc cggatctggc aaccacgctt gctcctgcca
    12301 cctattcttt tgatattgcg atcaacgata tgaactatga attccagttc aatattggcg
    12361 agggtgatac gaaccggcag atcacggaac gtctgtcccg tctgatcaac aacgcggata
    12421 tcggtatcac agcagatgtg acggaatccg attcccgtta cgcacttcgt ctgacctcgg
    12481 atgctaccgg tgtaccgaac ggcaaggcct atcattttca ggtcagcgat gatcatacca
    12541 gcaagacctc cggtgttgtg gattacctcg gactgaatta tgtcagtcgt cctgccggca
    12601 acgcaagatt ttatctcaat ggtgaagaac gaaccgcttc ttccaaccac tttacaatag
    12661 gaaaactgtt cgatgtacag ttaaaagccg tcagtccgga ggatcagccc acacatgtcg
    12721 gtctgaaaac agataccgaa tccatcacag acaatatcat ccagctggta ggcagttata
    12781 atgaatttat taaaacagct tcctcctacc tggagacaca gtccagaagc aaacaactga
    12841 ttcacgaatt ctcctccata gcttcccact acggtacttc tctggaaaac atgggaatgc
    12901 acctgcagga ggatggtatt ctatctgtca atgatgaaaa gctgcggcag accgccgcgg
    12961 aatccgggaa tgacctgtcc ggcttcaatg tactaaaaga attcagcagt tcgcttttac
    13021 acaagagtga tcaggttgct ctgaatccta tgaattatgt agacaaaaaa attgtggcct
    13081 acaaaaatcc cggtcacaat tttgccagtc cttacattac cagcgcttac agcggtatga
    13141 tgtttaacag ctactgttga ttcccttccg gggaattaac agtagctgtt atttcatttt
    13201 ctgcttctgt tttcttattt tgcctttttg gatttggcct tgtacttatc aatctgtaca
    13261 aaatcatcca tcagctccag aatcttgtct ctacctgtct ggttcagctt cacgtagttc
    13321 tgcataacac ggttttccag aagctgtgct cccagagaag catctctctc cagagaagag
    13381 aaatccaccg ggttcaggct cagcttgtca cacatcttga tcactgttcc gtacgccatc
    13441 ccttcaatcc cgcgctccaa tgcagtcacc agtgtgctgt agggaatgtt catctccatg
    13501 gcaaactgtc tgacactacg atactgtctc aaaatttctt tctttaaaat ctccgcctta
    13561 gtcatattta ttcctccacg tatcctccaa aattggtaca gctataatat acgatttccc
    13621 gttttccatt tcaagtaaaa aacagaaaat taagaatttc gacgaatttt taacaatatt
    13681 tttatatttc ttccaattga tccagccaga tcctggcaca ggcatcggac ggcatccgca
    13741 gatcacctct gggtgacaat gccacacttc ccactttcgg tccgtccggc aggcaggaac
    13801 gcttaaactg ctgtgtgaaa aatctccggt aaaaattctt cagccacttg cggatcgtcg
    13861 gatcgtcata ttttccttca aaggtctctc tcgcgatccg gtagaccttg tccggtgcat
    13921 aaccgcaccg cagcatataa tacaggaaaa agtcatgcag ttcgtaagga cctaccagat
    13981 cttccgtctt ctgtgcaatg ttcccctcca ccggtggaag cagttccggg ctcaccggcg
    14041 tatccagcac gtcctttagg actgcggtaa gctctgcatt attacaggta tcggcataat
    14101 aggccaccag atgtctcacc agtgttttgg gcactccggc attgacgcca tacatggaca
    14161 tatgatctcc gttataggta gcccacccta aggccagttc cgacatatct cccgtgccga
    14221 tgaccagagc attttccttg ttggcaatat ccatgagcac ctgggtacgc tctctggcct
    14281 gtccgttctc ataggtgaca tcatggcact ccatatcctg accgatatcc gtaaaatgtc
    14341 tggtgactga ttcccggata tccacctccc gcaatgtagt tcccagcgtc ttcgccagca
    14401 gacaggcatt ctcataagtc cggtctgttg ttccgaaaca gggcatggtc actgcaatga
    14461 tccccgacct cggaagtccc aacagatcaa aagtgcgcac tgtcactaaa agtgccagtg
    14521 tggaatccag tcctcctgaa atccccagga ccgctgtctt acatccgata tgctcatatc
    14581 tctttttcag gccgaaagcc tgaatggata aaatatcttc acatctgcgg ttgcgttcta
    14641 ccatgtcctc cggtacaaac gggtgagcag cgaacttccg gtccagtttt gtcccagtca
    14701 gctgcatgga aaaaggaact gtaagatagg gcttcatcgg tctctttccg aaggtaccca
    14761 ttcgcctgcg ctcactgctt atttttaata catccagatc tgtgtaaaga attcccgtag
    14821 taaaacgagt ggactgcgcc agcagtgttc cgttttccgc gatcagatca tgtcctccga
    14881 ataccagatc ctgcgtggat tccccttctc cggcagaggt atagatatac ccgcacagaa
    14941 gtcttgcact ggaagctttt atcagaaggg tacgataatc atccttggaa accgtctcat
    15001 tggaggccga cagattcgca atgactgtag ctcccatcag tgcatgcgag gtagaaggcg
    15061 gctctgccac ccacagatct tcgcagagct cacatcccaa tatcagctcc ggaagttcct
    15121 cacaccggaa caacaggttg gatccgaaag gaatcacctg ttttcccaga cgatattccg
    15181 tcaccgtttc ctctcccggt gtgaaatgtc tgccttcata gaactccgca tacataggga
    15241 tatttttctt aggaatcatt cccagtatcc taccccggta caataccgca gccacattgt
    15301 acagacggcc gtccttgtcc acaggaagtc ccaccagaaa taacgtttcc aactgtccgg
    15361 tctcttctgc aatcgtaagt aatgcttctt ttgctttttc caacaaaagt tcttgcaaaa
    15421 aaagatcgtt acaggtgtat ccggtaatac ataattccgg aaacactacg atctttgctc
    15481 catgctccgc cgcttctttt gcctgttcac agattacctg cgcattatag accggatccg
    15541 ccacccggat cctgggtgtg gcggatgcta ccttgacaaa tccctgtttc ataagcttcc
    15601 tcgctttact gtatacatta ctggatcatc tgtttcaatt catccacggt aaaattatag
    15661 tttttattac agaagtggca gttcacttcg atggtctctc catcatcaat catggactgc
    15721 aattcttccc tgccaatgct gatcagtgct tttctcactc tgtccttgct gcaattgcag
    15781 gcaaattcca caggaagtgt atcggtgatc tccagatcca ttccggaaag cagtacttcc
    15841 agcattccct ccggagtctt gccggcatcc agcatagcag taacggaaga gagattcttg
    15901 atattctcct ccagtctgct gatcacttcc tcttctgcga agggcatcag ctgcacaatg
    15961 aaaccgccgg cctgcctcac agtattgtct ctgttcatca gtaccccaag acctaccacg
    16021 gaaggcacct gttcggatac cgcaaaataa taggtcagat cctctgcaat ctcgccggtc
    16081 tgcagctcta tctgtcccac atagggatcc ttcagtccca tatcgcggat cacgcttaaa
    16141 acacctgcac ctacggcacc gcccacatcc agtttcccct gggcattggc tggcaggatc
    16201 acatcgggca cattggcata gccctttacg tgaccgttca tatctgctgt aactgtcagt
    16261 ccgccaatcg gaccatctcc tttgatctgc agtgtgatca gttcatcatc gccctttaac
    16321 atgcttccca tcatggcccc tgcgctgagt aatctcccca gtgccgccgt taccacaggg
    16381 ctggtattgt gagatactct tgcggtctcc accgtctctc ttgtcgtgca ggcgaacgct
    16441 cggatctgcg catttgccgc ggttgcacgt accatataat ccttcattta ttccacgtac
    16501 ctttctgtct acagctcttt ctgctgctct tttgctacga tatatactct ttcactggtc
    16561 tccgttaccg cctcaccggt atcggaatcc atgatctcca gaatcttcag tccggcctgt
    16621 tccaccagtg tcctcatctg tcctacggta taacccctct gcaaatgggt ctcggtaaat
    16681 ctgtggaagg tatcaccctc ttcctggacg aagattgtca gatcatactc gttgatctct
    16741 tcttccggat cataataatt ttcccagata aaactgcaat cctcccggtt ctccgcaatc
    16801 gtcgtattgc cgatgacttc ccggtattta taatctgtat taaaatcaaa gataaaaatg
    16861 ccaccggggt agagataatt gttcaccagc cggaatacct gcaaaagttc ctcttcttcc
    16921 aaaatataat tgacggaatc acagacgctg atcaccgtac caacagtaga atacagatcc
    16981 agttcccgca tatcctgatg cagatagagg atctccgaac cgctctccac ctttttctcc
    17041 atggcaatgc ccagcatctc ttcagaattg tccacgccga tgcaatcata ccctttttgg
    17101 tataacagtt ctgtcaaagt gccggtgccg cagcccagat ccaccaccag attccgctcg
    17161 gaatccagaa ttccttccgc gtcccgcgta ggtctggata caccatagga cgcaatcagc
    17221 tgatccagac gattgcacca catttcatag ggtgtattat ccatcagttc gtcatatact
    17281 cttgcaaagt ctgtatagat ttcttccgta ttctccataa ttgcagcatc tccagttttt
    17341 ttagaaaata gtccccaatg cgccgaatac tcccatgctg agcaatccca tgatcacgcc
    17401 tgccagaacc acaccgcaca ttactgcaat gacactggac ttgaaatcca tgtctaacaa
    17461 acttgctgcc agtgttccgg tccacgcacc ggttccggga aggggaatac ccacgaacaa
    17521 caacagcgcc acgaacaggc ctctgcccgc tttttcctgc agcttttgtc cgcctttatg
    17581 tcctttttcc aggcagaagg taaagaactt gccgatgacc ggcttgtccg caccccattc
    17641 cagtactttt cttgcaaaaa agaagataat aggtaccggc agcatatttc ccagaataca
    17701 gataatatag ctgggaagca aaggaagatt cattccaacc gcaataggga cagcacctct
    17761 cagctcgatc agaggaacca tagataccag gaaaatcatc aaatattttt ttaacatgtg
    17821 tttactccaa ttcttttatt ttttaattat acccgctttt ccagatattc tttcagaaaa
    17881 gcgatcagac ggtccatttc ctcatccgta ccgactgtaa tacgcaggga gttggcaatt
    17941 ctcggtttat tccagtgtct gacatagatg tcagcctcac ggagagcccg gaacagttct
    18001 tccgcgggaa cttccttatg agaagcaaat ataaagtttg ctttcgaatc cgggaaagta
    18061 aagcccaggc tcgtcagttc cttcttgatc cgctctctgg tagctgcgat ctttgccgtg
    18121 gtggccttga aataagcatc atcctttacc gcttccacgc ccatgacctg ggaaggcagg
    18181 ttcatggtat aggagttaaa ggagaatttt acatcattca gatagtgtat cagcttctca
    18241 ttaccgatac agaagccgat acgcattccc gccatggctc tggatttgga aaaagtctgt
    18301 actaccagaa gattctcata ttttttaagc aagggcagtg cacttgtgcc tccaaagtcc
    18361 acataagcct catccacgat cactaccacg tccggattgg cctgtaagat ttcttcgatc
    18421 attgcctggg actcttccac accggtaggt gcattgggat tcgggaagat cacaccgccg
    18481 ttgggctgtt tgtaatcttc agggttcatg tgccagttct catccagcgc acaggtatga
    18541 tacggaatgc gatacagatc cgcccataca tcatagaagg aataagtcac atctgggaac
    18601 agaaccggct ttccgctgtt gaaaaatgtc agaaatgcca tggataatac atcatcggat
    18661 cccacaccta caaatacctg ttccggtttc acactataat atgctgccaa ctcgtccacc
    18721 aggggctgcg catttgaatt cggatataag cgcagtgccg cactctccat attttctgct
    18781 gccttccgca ctcccggggc aggaggataa ggacactcat tcgtattcag cttgatcatg
    18841 ttcgggcggt tcggctgctc tcctgccaca taaggaacca ccttacgtac gttttcttcc
    18901 cacttcatgt taataaccat gcctttctca aaacctgcaa gccttcagag actttcgtcc
    18961 cttgtgcagg cacaaatttg cgtatgaaaa atttattttc agttatctgt ttgttcattc
    19021 tgtcgatgtt ttaattcttc cgcatagctc cgggcctgtg caggaagtcc cagctgctca
    19081 taacaacgga tcaatccctg taaaggttct tcttccgaac tccgcagcgc cagtaccggc
    19141 tgtgtttcat aagctgcatt ataataccag acagccgctt cctcaaagtc tcccgtctct
    19201 tcatagaaat accccagttc acagcagatt tccgaacagc cgtcccccgc gatcaccttg
    19261 gaagtatact tgaaaaatgt cacggcagct ccccgcagtc tggcagctct ggccaccaca
    19321 caacagcctt ccgtaatctc atccgcactt ctgtccgggg acgaaacttc tgccctgaaa
    19381 taatcttccg cttccagaaa gtcccgtgcc tctcccgcca ggaacagttc tctggcatac
    19441 attccgtaca gtcttctgga aagtcgtctt ccctgcgcga tctgtttgcg gaaatttgtc
    19501 agatccctgc ccgcatgatt ctgttccggc atgtgagtga tgactatatc gctgtcatag
    19561 actaccggtt ccagccgtac agtttcatgg attggttctt cccacacgaa gcttcgcagt
    19621 ctgcggaata atttgggacg atattcttca tcaaaattat acactgtgcc gttctgtagc
    19681 tgattgccgt atttcatctg gacgatctcg atctccggaa gcagtgtctc ctttaacagt
    19741 cgaaaccttc gccggttttc ttccgaaagc acctcatccg catctgcgga ataaatgtat
    19801 tccttcgtag ctttggaaaa tgcaaaattt ctcgccgcac tgaagtcatc gttccatgtg
    19861 aaatcatata ttttatcggt gtatccggct gcaatccgct ttgtggcatc cgtggatcct
    19921 gtatccacaa taatgatctc atccaccaga tccctgacac tgtccagaca ccttttcaga
    19981 acccgttctt cattttttac aatcatacac aaagatattg tgatcatccc ggatctccgt
    20041 tcccgcattt tttgcatgcg atgctgttcc tacagcactt tggataagaa ttcctgtaat
    20101 cttgggctct taggattggt gaagaactcc tggggagtat tctcctccac gatattacca
    20161 ccgtccatga aaagcacctt cgtacccact tctctggcaa atcccatctc atgtgtcacg
    20221 accaccatgg tcataccgct ctctgccagt tctttcataa cttcaagtac ctctcccacc
    20281 atttcgggat ccagtgcgga agtaggctca tcaaacagca ttactctggg attcattgcc
    20341 agagatctga caattgcaat tctctgcttc tgaccgccgc ttaaagatgc cggataagca
    20401 tctgcctttt cctccagtcc aactctcttt aacagcttca gggcattctc ttttgcttct
    20461 tcctcaccca tcagtttcaa tttcacgggg gcaagggtaa tattgttcat gatcgtcaga
    20521 ttgttgaaca ggttaaaatg ctgaaatacc atacccatat gctgacgaac tttgttgatg
    20581 tcgatcctgg gatccgtgat gttctgtcca tcgaaccaga tctcaccgct ggtaggtctc
    20641 tctaaaagat tcaggcagcg taaaaatgta ctcttaccgg agccggaagg tcctaccacc
    20701 acgatcttct caccggggtg aatatgataa tttacccctt ttaatacctg attatcttca
    20761 aagcttttat gcagatcttt aacgattatc actcttacgc agtctccttt ccagttttgc
    20821 aagcagaatg gacaatacct tggtcagtac aaaatagatg aaggcaatac caagcagcgg
    20881 tgtaaataca tcaaacagct tggaggtcat ctggttggcc acacgggtaa ggtcactcat
    20941 tcctacgtaa cctacaatag aggtttcctt aatcagcacg ataaattcat tgcccagagg
    21001 cggcaggatg ctcttgaatg cctgcggaat gataatgtat cgcatcgtct ggaatttgtt
    21061 cagtcccagt gatctgcccg cttccatttg tcccttgtcc acagccagga tgcctgctct
    21121 gacgatctcc gccacataag caccggaatt gataccgaat gtcagactgg cgatcagcac
    21181 actgttctta caggtagcca gaatgccgga ccacatgatc aacaactgta ccgtagtagg
    21241 agttccgcgg ataatatcaa tataagcact tgcaatccgg gacgctatgg tcttcgatcc
    21301 gttttttctc gtggataatt tcaaaaatgc cgctatggtt ccgatcagga taccaagcag
    21361 tgccgcaaaa aaagaaacct ctaaggttac tcccagtcct ttcagataaa gcttccatct
    21421 gtccgcttca atgaaggctg cataaatatg ttgccagata ttttgcatgt tatttcctat
    21481 gcctttcttg tgaaaatata agtgtacgat tggtcaaacc gtatatgttg cctaccttaa
    21541 tatagcaaaa agaagagaat ggcacaagcc attctcttct ttttcatgta tattttgtat
    21601 aaaaattatt caccgatgta ctcagctacc agtgcatcaa aagtaccgtc agccttcaga
    21661 tcagccagtg cctgatttac ctgctctacc aactcgcttc ccttaggcag tgcgatagcg
    21721 tattcctcag cttcgaagcc gaaatcagca ccgtcgatga tcttaacctt accctcgaat
    21781 ttggtattaa atacttctgc agggttctta tcaatgataa cagcgtctac tctgccgttt
    21841 accaaatcgt ttactgcatc gattgctctg ttgtaggtct gtgcggtagc accttcgata
    21901 tcctctgcca ggaacatacc ggtggtaccc agctgacagc cgatggtctt gttcttcaga
    21961 tcatctgcag ctgcgatctc ggaatctgcc ggtacgatca caaactgtac agcttcataa
    22021 tacgcatcgg agaaatctac ctgctcctta cgatcatcag taacggtcat tcctgcgata
    22081 gcaccgtcga ttctgttgcc aatggaacta accagagagt caaactccat attctctacc
    22141 tgtacttcca tacccagttt ctcgcctaca gccttgatca gagctacatc gaacccgtcg
    22201 ggttctccgt catctcctac atactcaaag ggagggaatt ctgcgttggt accgaatacc
    22261 agtacgccac cctcggtctt atcagcggcc tcagtagtag ctgctgcttc tgtagtggtt
    22321 gctgcagcag tctctgccac actgctcccg gaagcctgag agctctcgtt tgctgcggaa
    22381 tcagaaccac agccggtcaa cattgctgta cacattacag ttgcaagtaa cattgcgatt
    22441 gcttttttca taatattatc ctcctgtctg ttttctacgg acgacagccg tccttaaacg
    22501 ttgattacta tagcaccttt ttgaaaattg tgcaactgtt tatgcataaa tatacgcagc
    22561 tttactctgc aaatacaaca ttaccgggat ctgcacccag aaggttacca ctatatatgg
    22621 aataaacggc ttcggtacac gaagttttcc atccggtgtc acatttcttc tgcaaaactg
    22681 tactgcagcc agcaatgtca acgccgataa catatggatc agaaaccaca acagattccc
    22741 ctgccaggcg cagcctgcca acgcacatac agaaaatgca tgacagtcag ctcttccgta
    22801 catttgggca aacaatatct gctgcaggag tacaaacaat gccactgctg ccgcctgctg
    22861 tatgctaaac ctgtcagtaa tcctgcacac tccatcctcc atataagctt ccgacaccgg
    22921 aagccccagc agtattcctg tccatggcag gcaccaccac catacataat tatacacata
    22981 actgtgtaca gcatccatgc aggccgccgc taacaaacct cccgccagca gggagcacag
    23041 gatctcctgt gcccccagga tcaccagtct gttttcttcc ggaagtcttc catccattcc
    23101 ccctataaca agaatcacaa ctgcattgat ccaggccagt cccccgagaa gccacgcttt
    23161 tccgggttcc atcctctcga tgtctcccga tagttccaca cgcagcagcc agattgcact
    23221 accatagata aggcctccca gaaccaccga ccacagtatc cggaacacga tttccaaggc
    23281 tctcatacct ccttaccgta accgggtacg cagttcatcc aacacatttc ccgacactcc
    23341 gatcacgctg atcgtcggat gatcttccag cctcttgtca cctttatagg cccgcactgt
    23401 gatttcctgt gtctggttct ccggagtata taaaggcacc ataaattcca gttcttccgt
    23461 cttacgatac ccctgacttt cggtataatc atagacttta ttcaatgtcg gatccagtgc
    23521 tgtcatacat tccgggaaaa tgacttccac ccggtccgca tatccccatg tggtaaaggt
    23581 aagaattccg ctttcgccgc atttgaagac cggctcctgc ggctccaaaa tacgtttcac
    23641 actgctttcc agtgcaaatt ccgtcgtacc gtaacagata cggctctcat tccccacatg
    23701 atccgaagca tatcccagta ccgtaaaatc cccgctgaac agcggttcgt cctctgtgat
    23761 tgtaatcacg atacagccat cttctccggg aaggtaggtc ttagaaacag cattgtccgt
    23821 attactgata acaattgtaa aatgatccac accacttaga gcatctgtgg cagtaacacg
    23881 tatggtaaca gctccgtctc tgcgatcgat cagtgcccgg ttctccagta tttccatccc
    23941 ccggatgacc ggagcttctc cgtcagcgat cagagcgatc ccgtttttct gatcctgcgt
    24001 agatacagaa tatattttat ctgttccgtg atccgcttcc gctaccggaa tcacccgaat
    24061 cgtttgtccg ttccggtctc cacttatgac aaattgctgt actccggtaa gttcctgatt
    24121 ccggtgtgac cgcaaagtat aggaatagga atgttgctgc agcacattgg tcccctcagt
    24181 cgcataagac agtccctccg catctgtccg gatcacatca tccgttatct ccgcagacgg
    24241 tgtgacaatg atatttctcg ccctgccacc gtcctccgtc accgtttcat atattgtccc
    24301 attcagttga tagtccggtc tggctgtacc ttccatataa gatgcattcg ttaacgtgaa
    24361 cggtgtcttt ccgtccgccc ggacatacca ggttttgtcc gctgccgccg gatatacatt
    24421 gccctctgcc tcgtttatct taagctgttt tgtatgaagc ttccacagaa tctgttttcc
    24481 atccacacaa atatgtgtgg tctcgcttac atttcccgcc acatccacag cggcaacatg
    24541 cagatactgc accccatcct ttacctccag ttccatatgg ggttctttac aataaccggc
    24601 atcgtccgtt acgacggtat ccctgttttg atccacaaga taataatatc ccctgatacc
    24661 gctaagcaat gtgttttttg tgatattgga gtcacatagt ttggaagtac tgcccgtcag
    24721 ataagattcc gctctatgat aatataccgt cccgtaatcc tccggctctg tccacgagat
    24781 tcttattctt tctctgtcca gtgcttctct tacgacgttc tccggaattt tcccgggagc
    24841 cgcaagatca gttgccctga ctcccttcag ctgtaaagtc tcctggtaac ctattgcttt
    24901 tgactgcaga cacaacagcc cgtctccact ccgtactcca ctttcacttt catatagcag
    24961 tgcacattcc ggatttacat aattgcttcc gccatatgcc ggcttcgaga ataggacctg
    25021 gccttccgta taaccgcata caagagttct cccaccactg aaactaagtg agctgattcc
    25081 cgcggtaaac cagacatttc ctcttaaatg aaaattcaga tgcacgtaga tatgtgtcgt
    25141 tccttccggt aatgagatat gcagggactg ccggaaatca ctggctgccg ggtctccgcc
    25201 gccatgttta tcataaaaag gataatcccg aaattttccc tgtgcaattt ttgttccttt
    25261 ctgatccagg attacaaatt ccgacggctg cttctcatcc ggcagccagt cggtattcac
    25321 aatatttcca cccagagatc cccatccatc gcaaaaatat gaaagattca atgtcgtatt
    25381 gccattggtg ggaatccccc gataaccgga aacattccac atctgtgcct taggataatt
    25441 cccgtttctg gcccatccga cccggactat ggcacggctc cactcatcat cgtccgtact
    25501 tgtggtatgt ccgttatact gataagaatt tgtgcctgag gaatcaaagc aattcgatcc
    25561 ccacataaaa tattcccaat ctccgaatgt tggtgtataa gaagcatttc tatagcagtc
    25621 attcgtatgg tgatgcacga tcttctctcc ggcggttcct ccataatatc cggctccgcc
    25681 tccggccata cccgcttctc cttcctgctg cccgaggaca ctttccatgc tgccaccctt
    25741 gccgcctgca ccgcccggtg atgctccacc gcctcctcct gcgatcagca ggattccctt
    25801 ccggtcggat accacggatg tcattcctcc tccgtttcca taatttgaag ctctgccgcc
    25861 tttgttatac ccgttttgac cacccacggc ataggtcagt tgttcccccc tctgcagcca
    25921 gaatgttccg gaggcacttc ccccacatcc tccccgaaag gaaccgtagc actgtccctg
    25981 cgcccccatc gcctttatga cgtagagtcc ggtatacggc acgttatatg tcctcgtctt
    26041 tgcttcataa ataccggtca gttcaacggc cgctccgctg ttgatatccg ttgcactatt
    26101 aatccgcatc caggcaccgt cttccagtct ctgataaata aggtacgttt tgttgttgtg
    26161 atccgtctgc tcccaggaca gatccaccgc tccttttcct tcgttttcct tgtagttatc
    26221 tttcgccagc agggtaagat ccacccattg cgcgtacaat gtcgtatttt cggacggtat
    26281 gatgctgtcg cccgctcctc ctgccggccg tcggaattcc gagtcataat accagcctcc
    26341 aaaggaccac ccgggtctgg tggtctccgg taatgtgaca ggttccggct gatagactgc
    26401 cgtcagcctg tccacattac cgtccggagc atcaaagata tattgctttc catccagact
    26461 ccccctgaac ggatgcacct ttttccattc gacaaaccga tttttccctt gcaccggagt
    26521 gatctccgat ccgccattgg cacgaaaaca gactgtatac ccatctggtg cgcagaccag
    26581 ttcttcctgc aacagatatc tttcaagata tggctgcgtt acagaaaata tctcccggtc
    26641 atcatacttt cctccattgg cataaagcac aagggtgctt tcactccgca cccattgtgc
    26701 atataaattg actacaccat gatcgttatc tgtccaggtt gtccggtctc tccaatccgc
    26761 ctgcctgaga ttttcgattg cagctttatc cgcataagcg gttcctgaac catctggctc
    26821 tgtgttccag cccgcgaaca aatatccggt cctgctgtaa ttgttctctg tcaggtgtgt
    26881 caccggagtg accgtcttgc cttcgtagac tatggcatcg ttgtacatat ggatactgtc
    26941 atccatataa ccgttataag atgcacctcc cgtattggcc tgataacaga ccttatactg
    27001 attccaggaa atatttttac atttatgtgc cggcagatcc accccctgct ccagattgtc
    27061 gcagaacgga cagagataat agtagctcat cggttttctt tcgttgccca gatccaggta
    27121 ctgtatcgag gccgccgctt ctctgctcat atagatattg tactcttcaa tggcttctcc
    27181 acaatccgca caataggctt tccagccgga ataataatac tgcatgcatt tttgtctgcc
    27241 aaaagatacc ccgtgccagt tttttccggt atatccatta tgaatacact gtcccggccg
    27301 gtggaccact ttccaatatc tcactgggat ttctccatac cgtttctctg tataaatatg
    27361 ttctcccact accggatctc tcagcgaaga aggaatctgc gtatacacag tggtgctgtc
    27421 atcccgggca taccacacat aattccggtc tcctgcgcag gttgtaaatg cacgtccatc
    27481 cggactgaaa gaaacataag gactgttgta aatggtcttt ttcgctgccg cttccgactg
    27541 tatcgaaaac aggagcgcaa ttcccagcac gagtaatcct gccagatatc tcccataccg
    27601 cattctcatt cctcctgcct atccgtatcc tctgccttga ccacacgcaa ggagccctgt
    27661 gccagtgcag cataaaagtc cgccaccctt tctttttcca tatagatatt tactctctgt
    27721 gccggagttg tcccatcatc accgccgatt gcagctccat tccggtcaaa cacctgttgg
    27781 acaaacacat tttcccacaa cagtttcccg ttttgttcct cttcctcctg ttccacacaa
    27841 taaatatgga tcctgtctcc ggcacgcagg acaccgccca ctacctggta cagatcttcg
    27901 gcgcggaatc cggctataac aggttcccgc atctgttctg tgatctccga cagttccagc
    27961 agcatgcctg tagtcagtac catcccctgt tccactccat atgccgctat tcttccgctt
    28021 gccgcctctg tgcttttcag tgcagtctcc ggcacaattc ttttgtcgat ttccatgact
    28081 gtaatatact gctctgcatt ttccgcggag attttttctc ccctgggaat tgctgccgtt
    28141 accacgcata cctgttcttt ttcgtattcc gtaagcactc tcttttctgt ctgcagcaaa
    28201 aaaacaaata ctgccatagc cgccacaaac gctgccagga tacttccgtt ccagattcta
    28261 ttcttccacg cctgactcat cttccgtttt ttcattcgtt tcctctccca tatattcact
    28321 gaccacatct accagcttac ttttttcagc agttactgta attcccagaa aaccttccac
    28381 cggatatgtg atctcactgt acacaccggt attctcaatg gtctgtcccc ccggagtcct
    28441 cacttctccc agggttcctt cccattccag gatccttccg tcaccgtttc gttcccacac
    28501 ctgcactttt gttcccgtga cattatagat aatgtaattt cgtatctcta cctttccgga
    28561 aatcagcttg tgacctactg cttcataatt ttcatttaac cccagatttt cacggacagc
    28621 agaacaatat cccgcatacg cctgttcctg atctgtgata tgtaccttat gtgtgctgcc
    28681 atactcccgg atgtcaatga ctgccgatgc cagattggac agtgccaatg catcttccag
    28741 ataccgtgcg gatgccttgt acattgccag ttgcagctgc atatacaaca aaattgccag
    28801 aaacagtatc agaaacaatc ccagcaccca ggaaacctgt ccgctttctg cttttcctgt
    28861 ctcctgccgg ttctcagttt ttggcagtcg atactctctt ttcccgtatt tcataatgtc
    28921 cgcccagctt tccgtgaatc tgcaggataa tgcgttcccc atatcctgca ggttccaaag
    28981 tacttccctc ataatcgatc tctgtcagtc ctgccgtctc cagttctgcc gtgaggtgtg
    29041 cctgctccgc cggttccaga tatcctaccg tttccatttt cagcagatag gccctcgcca
    29101 gctgtcctac attctctttt gcctggagca gctgcacatt ctgcaggtag ttcagcatca
    29161 ctaccgtcag tgccagcaca cacatgccca tcgtcagcag ctctcctatg ttccctggct
    29221 gtttccctcg ttccgtcatc ttctctccta tcctataccc ctgtcaggat atctgccgct
    29281 ttggtctgca tcgctgttac aatggtctgt ataaacgttg tcagactgtc cttcatgacc
    29341 acgcacaata acagtgcaat aatacacaga cctacagtga ccaggatccc gtcgatcccc
    29401 tgctcctgcc gtaaggcatg ttcttttact tttccccaga ttctctgtaa attcttcatg
    29461 ttttgtccct ttcttgtttt gttttattgg tatttcagaa ttgcaccact gcccggaaca
    29521 tatatcccac acaggcatat aaaatgattc ccagtaccgc ccccagcatt cccagctgat
    29581 aaaaagtaat actacggtcc agagcacttt tttgctgttg aagcaccgta agttccatat
    29641 ccttcaactg ctctcccatg tccttcaaca gatctaccgc ctgccccgac tctcctgcat
    29701 ggatcagggt aatgcacaat gcatcgatct gacggttgtc aaatttctgt tggaagtcag
    29761 acaaagccgt aaccagatcc gactgtatga ccagatctcc ggacagttcc atcaaagcct
    29821 gctgtaatct ctgcgtccgt acgctctggc agcattccgt caaggcatcc gttacataga
    29881 ctccggcaca ggtctgtacc tccagtgcct gatagatcag ctgcagatcc ggcatcatct
    29941 tatgattgtc ctgtcggttc aggtacagca ataacagttc cggcacataa tacagtccca
    30001 ttgccgcaag cagtgcatat ccccagccga tagattccag tgtcagtact gcgccaagag
    30061 aaagaaacag gcgcatcagc aggtatttta ccggtgtgat tttcttccca tagtgaaatt
    30121 ttgcaccgtt tttccttaat ttcttttctg ttccggtgac ccagttttca taccttcggc
    30181 tctcccgcag cattccggta aattcctcgc aggcatgcaa caccgttttc ggcattgttc
    30241 ccatacccag ccggaacaca aaaattcctc ccagcaccgc tcccaacagt tcgggaatcc
    30301 agataactct ccagctcatt ctccacacct ccctgactag cggcgaaaac catacagttt
    30361 ccgcaaaaac aaaaacaata tcagtccata gacacccact gcaagatgcc ccggtaaagt
    30421 cccggatact attttccaga ccgacacatc gatcagccga tccaccgtca gcagggcgaa
    30481 taccgacatt cccagaagca ctgccatatt gatacctgcc tctcgaagca tccctttacg
    30541 ttcctcttcc atacgcagat actcccttgc ggaacgtctg ctgctgtcta ccagtgtggt
    30601 cagatctgcc atgtagcgga tgctgatctc cagatttctg gccagttcct ttatcttggg
    30661 atgttctatt cgttccgcca tggacaaaag tgccagcgaa ctgtctccgg tcgtctgtgc
    30721 ttcataggcg cattcctcca gtgctccttt taacggctcc tccacataac ggctcacctg
    30781 acccagaacc atggtgatct ctcccgctgt cagactgtaa ttccccagaa aattcagaca
    30841 tttcagcaga ttttcattgg tcacccgcaa ttcttctgcc cgcagtgtgc tcaggaacag
    30901 atattccatg gctccgcata tcattattcc caataaagct acctggaatt tcccgctgct
    30961 tccaagcaac actccaaaga tcaccgtcat tcccagaaga tttcctacga tccatttctc
    31021 cggggtgcac gccggaaatc tgcgccgcat accgcaataa cagagaaatt cttccacccg
    31081 gcagatccag gtattgttac gctgtagttc ctgcagcttg tgccggtttt ccaatacccg
    31141 cagtcttgcc gactgatcca tccctttttt cgtccgggac agcaggcgtt ttgccgtctc
    31201 tacccaccgt atccttccaa acagaagcag aaaacccgta aatattaaac aaaagatcca
    31261 tacgtatatg atcataagtc tctcctctca tgtaatggtc aaaacaatct ctcatactcc
    31321 agattctctt tctccgcatt ccaccccaga caggcgaaga tctccttcac ctggtaatgt
    31381 tccataaaca ccagggtctg aaagcagtcc atcattttca ttaattcgtt gcgggaataa
    31441 cgactgtcat acattgcata gtccacgatc ttctccggtg ccctgtccgc agagggtgca
    31501 tgtaccgtag ccgcacagat ctgtcctgta tacgctgcat tcaacagata caatgcttct
    31561 cctcccttca cctcacctat gataaaaaaa tccacgtcca tcgtcagtcc cgctatgctg
    31621 atatgtttta aatcataatt cacactgctt tccgaggttc ccggaagcga atgaaggaac
    31681 atcatatccg gatgaaacag tgtggtcagc tcatccgcct gctgtgccac cagaattgcc
    31741 atatcatccg gaagcgtctc cttcaacgca tttaacagtg tggttttacc ggaagaattt
    31801 cctccgcaga tcagggtcga tccctgtcgg aacctctgca ccagcagttc tgccgtattc
    31861 ctgtccagca tctctttctc caccagttct gtcatcatgg gaaaactctt cggcaccttc
    31921 cggatacaca gataaggttc gctgtaggta ttgacaatgg gcatggatag ggtaaaacga
    31981 aagataaagt ccggatgact ttctgtatcc gtaaaccgtt ggatcgcatt caggttagaa
    32041 atattcacct gatttctggt cgctatataa tcaataaact gtcgatattc c
//
LOCUS       DAWDHD010000010        30528 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_124_length_30528_cov_14.300397, whole genome shotgun sequence.
ACCESSION   DAWDHD010000010 DAWDHD010000000
VERSION     DAWDHD010000010.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 30528)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 30528)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..30528
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124_length_30528_cov_14.300397"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         7728 a         7955 c         5862 g         8983 t
ORIGIN      
        1 cagtagcccc ggctgggtat aagaataagc agtgccgtaa gcagacgtcg gtaccatagc
       61 gtcccaaggg acgcattgcg gtcgtattgt gaccgcttat gtaccgctac gctctgcgac
      121 aggagcgcat cgtactaaag tacgagcgtg ccgcgtttct tataccctgc caagggctac
      181 gaggatattt cgctaactct caattttgca atctttacca tcaaagcagc agatatgtct
      241 atggtgtaca aatattatga aaaaagctga tcttcctcct actttttatc ctttcgcaaa
      301 tacatcaaaa tcccatgact cactctgcca agacatgctc ccaccactgc cccggcaatg
      361 gtgattcccc atatttccac atagcgaaat ctgtttgccg aaaatgccca tacgcatgct
      421 acgacaccgc acataacgat ccatccgata tagatatctt ttatatgctc attctcccac
      481 gaactcccag atgacatcgc atcatttccc accagttcat caatgcttat gtcgaattgc
      541 tttgataaga gaattaattg ctccggattc ggcgccgtct ctcctaattc ccaatttgat
      601 atggtctgcc ttgtaacatt aattttttct gccagcttct cctgcgaaag ccccttcttt
      661 tttcttaagg tctgaatatt gtttcctaac tgcatccgtc acctctcctt tcgcatgaag
      721 tatagcatgc aatagctatg cagtactatc aacatctctg ggaaattgcg caaaggaact
      781 taacatttcc gcaaacctca gagtttctac tgatttgccg gtttctccgg tattcggcat
      841 tgtccacatg tttcgagtgt tcctatgtac catttcagga ttgctgccac aaaattgcta
      901 ctaccaaaaa cataattgca aaaccaaatt caatcactgt cgtgaacctg attcttctct
      961 tcaaaacaca tactcccgga tgttttgaat gaatatagat gggataagtg ttcccttctg
     1021 tcatcctccg cagcaggcga tagggtactg tcaatgtggt ctgctcatgg taagccgtgc
     1081 cgttacagac gtattcaaac accggtgcat agctggtaat cccatagcgt ccggggtagc
     1141 tgttatatcc gcaatagata gcttctatct tctgattgca gcaaaataca gcatagatat
     1201 cattcacacc cagtaaaaac agaaacaatc caaacacgct cataatcagc agcgcttcga
     1261 gagcttcatt ccctacaaac aaaataacaa atgatgtgat ggcaaaacca gcaaatgtca
     1321 ggatgatgtc cagacatata aaaggtacaa ccttatatct cttatttttt ataatgataa
     1381 cggtagatac tccaaacaat aatgtcaata tgtataccag agcttccatt accatatccc
     1441 tctcctccga tccaataagc aaaatttatg agtttttcat tacccaggca aatgccttcg
     1501 cggtccgcag gcccggatct ctgaaatgat tccccggatt ccattccagc gtactgccga
     1561 tcccacactg ctgcagccac gcatgtgcct cccggataca gtcacctaca gtagccataa
     1621 caggattttt cgtcttttcc tctttgtccc ccaggcttaa gtaaactgcc cgactctgat
     1681 tctcgtgctc tttcatatag ggcagaaacc ctgggaacca caccgaggac gatgccgccg
     1741 ctatccccgc aaaccgatcc gtctgataag atgcccacag agaaaacaac cctgcaagag
     1801 aatatcctcc aagataataa gttttacttt catccgagca taaacgcaaa atttcttcta
     1861 acaacgaggc tgcaccatcg ccaaaatcct cttttccgaa caccgccggt gatcgccacg
     1921 gagacagatc gaggttccag tcatccactt tcaccgcaat catttgaaaa tccaatgctg
     1981 tcagtttccg gatttgtgcc acttcgccct ctatttccgg aagatcatga tctcccacgg
     2041 gctggatcaa aacaatggga gcctccggat tcccatattt atatttagtc atgccgtcct
     2101 ctttctctat caaatatcct tttctatcaa gtatccattc tattttatca gtttcagaat
     2161 cctttgcctg ttatatcgct gcatgatcac aaacatcata tcaaacatgg ccgcaaggat
     2221 cgatgtaatg ataaatgccg gatatccacc cagccaatga cctgccacaa tcggtgcaaa
     2281 gctgagcact acgatcgtct catgccccag ttccgcctga catgtgacct gcgctatttc
     2341 ctcccaggtg tgttgtctcg gatcaaacag gctgggatta taggtaggca tacaattctt
     2401 ccattttttc acacgcagct tttcatataa tttcatttcg gatttactta cctgatacca
     2461 gtgttttctg taatctgccc tgttatgcat gatagaattg tagataaatg ctaccaccca
     2521 tcgcatgata aaatgataag cagtggttcc aaaggtaatg gcaatcgtca gccagatttc
     2581 taacaaagtt ttttgatata aaatagccgt gaccatggtt ccgatcagga acacgacagg
     2641 cgctgccttt aaaaaattta tcataggtca ttctccgttt tttccaactt catataccca
     2701 ttttcttaat gagttttgaa taaatatgtt ccacgaacca cggttccgta gatttctcca
     2761 aagcatcttc cggagagaac cacatcactc cactattctc atcggctttt acggatacct
     2821 gctcttccga atctgcctcc agtaaatacg tgatattcag atgcaaatgg ctggacacat
     2881 agttcccttt tttcacatgt ccgtccaccg tcagagattc cagagaaaaa atctcctctg
     2941 ataccggtct cacattggaa attcccgcct cctctttcac ttcccggatg gcaacggaca
     3001 gcagatccgt ctccccatcc gcatgtcctc ccatccagga ccaggaatta taaatattat
     3061 gatataccat aagcactttg cttctgtcct tatttaccac ccaggcagaa gccgtaatat
     3121 gcgcaactgt attttctctg gaaaatgcgt tctcattgtt actgatccat cccaggatca
     3181 aagctttgtc cttttcttcc tgttcattga atggggcata gttttctatt tgtctgatgg
     3241 aatcctctcg tgtcatttgg ttatctccct attttcaaac aaccggtcaa tctcctgcca
     3301 atccttacat ctcacaaacc tgtcattggg aagctctgcc cggctgttcc atggccggtc
     3361 gaacacggct gtcctgcagt tgtcaaaatg tattacatgc tcaaacgccg ccggagaatc
     3421 ctctaccgca aaatcaaagg tcatgttgta aagttcctcc aatgtcatat tatatttgta
     3481 gtcctgtttc gcagtttcgc gcccgtattt gtctacacag aacaagggaa ctctttccaa
     3541 atgatgttcg tcaagccatt tcctcgatgg ttcataggaa ttgaacggcc ttccggtaat
     3601 gataaaaacc tcgtgtccct gatccaccca cttattaatt gcttcggaag cccccggagt
     3661 ctcctcataa tgcaacaaat tttccggtaa atgccccgcc ttcattagag cttcatactg
     3721 ctcatcattt agatcaaatg atttctggag attaaaaaac tgaacctgcc ggtaaggaac
     3781 gtcaataccg aacatttctt ttgcaatttt cgtaaaatat tcagcggttt cacataatac
     3841 atcgtcaaaa tctatgtaga ttttcattat ttcttttctc ctataccttc gcattattct
     3901 tctaatagat ttttaaacac cattgtgatt tccgactctc ccccatggtt ccatttaaac
     3961 ctaacatcac ttcctgcctt tggtttttca ttaatatccc ccgccaattt tgcaggaatg
     4021 agtttttccc aataacgtac attatatata tcattttcta cagattcaac cacacctaaa
     4081 tgttgctcaa aaatctgcat aaaaccctcc ttaactttta ctttgcggta ctcgggaaac
     4141 tcttcactga ttctaagcaa cgctgcttca tcaccgagct tttcaaaatc agaactttca
     4201 atgagctttc ctctgattaa tgaaaaatca gcatttaatg gtaataccat tatttcatca
     4261 aatgattttc ccctggcatc tgtaatttta tatttggaag ctctctcaaa tccaaaacgc
     4321 ggataataat aaggttctcc aataattaca attccagcta ttccctgctt ttgggccagt
     4381 tttattgttt cacgcataag cgctccaccg atatcgtttc cttcccatgt tggttcaact
     4441 gctaatggtc caaatgtagc aatttcatgc ctactttttc cgtctacgat ccacgctttt
     4501 gtgtaatata tggctccgac aatctggcca tcaatttctg caattcggct tatttccggt
     4561 atgtattctt gagaatcacg aataatgcga gtcaataaat gctccgtgca tccgggccaa
     4621 tatttattcc aaaagcttct cattatcatt aattccgttt ttttataatc ttcttcggtc
     4681 tcttgtctaa tgattaaatt atttatcatt gtatcctttt ccactccaca ggcctcaatt
     4741 ttcctaacca tttaaaacta aaatttgtca gttgagttca ttttcctcct catcattgaa
     4801 aatatacttc gatgtttcct ttgcaaggat tttaatcttt caattctgcc tcaatctctt
     4861 caattgtagg caagcttccc ttaaattctt ctggtatcaa cttagataat tcatagctgg
     4921 atattcccaa aggttgactt gttgattcca gtgcatactg tgcctcgact ttatccatat
     4981 ctttacagat taacaatcct aaagttggat tatcattctc tgttttcatt tggtgattaa
     5041 ttgcgacaac atacgttcca agctgtccgg cataagatga gtcaaattta cctgtcttaa
     5101 tttctacaac tacatagcaa tgtctttgtg tattgtaaaa caacatatcg atgaatttct
     5161 ctacttcccc cactgtgata cggacttcgc gccccatata agcgaatcct gttccgagtt
     5221 ctattagaaa gttgtttact ttattaatta atgcatcctt taattccttt tcatcatatt
     5281 gttcccgtaa tgtcaaaaaa tcaaaattgt acggatcctt tgtaattgcc tgcgccaaat
     5341 caccttgcac tgaaggtaat gtatttgcaa aatttgttat agcttttcct tgtctttcaa
     5401 ataaatccgt atctaaaaaa tttaccagga catcacgaga ccaattattt tctagtgttt
     5461 ttctcacata aaacaatgcc ttatccacat tgtttttaca tttgtctatt attgttctat
     5521 tatgtcccca aggaattcta aaaacgatag tcgaatcgtc cacagcttgt ggacgatttc
     5581 cggcacaagg atataattca tagaaataac gcatatattt taaattggtt ggtgaaaatg
     5641 acttaacatc cggtaatacc ttttttaagt catcactaag tatcttataa aatcctgatc
     5701 catatccgta ttgcttgctc atggttgaaa tatcttttcc aagtttccaa taaaaacgta
     5761 gcatttcatc atttattttt accgctgcac gaatttggct ccttttaaat tcatttgata
     5821 tttctgttat ccaatctttg tatgattcac catcgacaat aaccaatttc tccattattt
     5881 cattcccctc tataaattat tttttcccgt ttcaccacaa cttttatcac cgcatccacg
     5941 atggattccc ccggctcgat atgacctccc ggaagtgtat atcccttcca gtcatttttt
     6001 attctgtcct gtaacaagta tctgccattt ttatgtatta agcataaaac ggtaagttcg
     6061 acgctttctg ttctcattta ctttctcctg caatgttttc gacaatttgg gaatctccac
     6121 tcttcctaca aagaatttcc acactgccat accattacat agtctttttc cccggtaaat
     6181 tcatccaatc cttctcgttg gatttcaaaa ccttctcgtt gataaaagtg aattgcacgt
     6241 gtattctttt ggtaaacatt taagtataat ttcgtctttt tttcttttac aaaattcaac
     6301 aggagcttac ctataccctg cgactgcatt tcatcacgga caaaaatgcc ttccatatat
     6361 tcatcattta accctataaa gccttctatc cggcagtcat tctcatacac atagacctct
     6421 gcctgtggca acatttcttt cactaaatca atattatctt tccaatattg acccggaata
     6481 aaataatgtg cttttaagtt ggtatctaac catatatcaa caactcgttc gatatctgct
     6541 ttttgtaata ctctgatcat acttcttcaa acaccctgtt ctgctacaat ttacaaaacc
     6601 acctcgaaga tcgtccctcc ggatggattg gacttcactg taatactgcc atcatgcact
     6661 cttacaattt cgcggaccag ggcaagtccc agccctactc cacccagctc ccggcttctg
     6721 gatttatcca cccggaaaaa cggttcgaac acgcgctcct taagttcctc agggattccg
     6781 gctcccgtat cctccactga cagatacaca tgtttttcct tccggtctgc ggttactgtg
     6841 acctgcccgc ccgaatgatt atattttatg gcattctcaa caagattgta taccagccgg
     6901 taaataagga tgtcacttcc gaccatcgtt atatccttgc attttccgat cagtcttata
     6961 tttttgccct ctgcaagagg ttccagatcc tccaaaacct catccaccag agcatctaag
     7021 atgatttcat catcccgtcc tactgtctga agctcactca tgtcgagaag tgtctttacc
     7081 attttgttga gtctgtcgtt ctgttcggta accatcttta tcatctgtac cgtgtctgca
     7141 tcattgtccg gatgactgtt ggaatgatac agatcaagct gtacctgcat cagggctaat
     7201 ggcgtacgaa gctcatgggc tgcatttgca gtaaattgtc tctgtatctc aaatgcttcc
     7261 gagaggcgtt ccagcatacg gttatacgaa acgctaagct ggttcagttc cttcacctgg
     7321 ttttcttcta tccgtgaatc tgccagattt tgcgcctgca ccttttcgat cttatcagaa
     7381 agctctctga tcggtctgag tgcatgccca cttataaaat aggtagccac tccacccaga
     7441 agagccaata ctgctgaaat gatcaagctg tttttcctgt aatccgcctt attattatac
     7501 acctgaacag aaaagtcatt ggcgaactca tcccatttgt cctccggaat gctgatatat
     7561 atttcctccg catcgtccgg ctgatcatct ccccgtgtat ccacagcctc ttgcagggaa
     7621 tccatataat atacaccgtt tttatagacg agcatcgtca cgcatccaca tatcaccgct
     7681 atatataatg taatgatgca ggtaagtctc cattgcagcg acattttttt catctatcgg
     7741 actcctctcg tattttatac ccttctccca ccttgttcag aatcggatca tatcccagca
     7801 cagccttaag cttttttcta agggaagaca tgtgtactct gatcgctccg ctaaagctat
     7861 ccacagatgc atcccagaca tgctccatca attcctcctg acttaccggt ctgcccatgt
     7921 tcagaagcag atattccaga ataccatttt cttttcttgt cagggatacc atttctcctt
     7981 ttgcataagc ctcacgcctt acggtatcga actttatctc accgctttcc agacatacat
     8041 ccttctgcac aaacttcctt ctggtcaggc ttctgatacg tgcctcaagc tcctgtagat
     8101 ggaatggttt ttccatataa tcatttgccc ctgcatccag cgcctcaacc ttatccgcta
     8161 tctgccccct tgcagataat ataagaacct tcgtctcctc attttcctgc ctgagttcct
     8221 tcaggatctc cattccatcc atgccgggca gattcagatc aagaacgacc agatcatagt
     8281 tctccgtcag gatacctgca agagcttctt ccccatcata gcaggtatcc acctcatatc
     8341 ctgcgtcata cagacttttc gcaactgtat cacatatttc tttttcatcc tcaacaatga
     8401 ataatctcaa aattccttcc cgccttaaca aagattttac aacaattatt gtataatagc
     8461 gaagtcaact ggtcaataaa taccacgatc gaaaggagta acaataactt gaagtgcaga
     8521 aaaatatctc agatcgttct gctcgtcacc gggatcacta tgatcggcta cggtgcagtc
     8581 agaggcgaag ctgctgtcgt gcttggtaaa gcaataaaat tatgtctgga gtgtgtcgga
     8641 attggataag agaaaaaaat cactgtcagg gcaggcagcc gggatacgcg gatggataca
     8701 ggctgctgcc acactgctta ctaatattca catccctaat ttttttaagg gaaaaatata
     8761 tcagggtaac gctaagacca tatgtgtacc gggattaaac tgttattcct gtcctgctgc
     8821 tactggagca tgccccattg gcgcatttca ggcggtgatc ggatcctccc gatttaaatt
     8881 ttcatattac gtcacgggat tttttatttt actgggtgtg acacttggaa gatttatatg
     8941 tggatttttg tgtccgtttg gatggtttca ggatttactt cataaaatcc cgggaaaaaa
     9001 attttccaca gccagactga agccgctaag atatctgaaa tatatgattc ttatcgtttt
     9061 cgttatcctt ctgccgctgc tcgtaacgaa ctctatagga atgggcgacc ctttcttctg
     9121 taaatatatt tgtccacagg gcgttctgga gggagccatc ccgctttccc tgggaaatgc
     9181 agccatacgg tccgcacttg gaaaactctt tacttggaaa tgctttattt tgatcacagt
     9241 tgtcgtgtta agcattttat tttacagacc cttctgtaaa tggatctgtc ctcttggcgc
     9301 gatctactca ctgtttaata aggtcagcct tcttaatatt aaggtagagg acagcaaatg
     9361 tatcggttgt ggtcaatgtt caagggtatg taaaatggat gtggatgttt gcaggcatcc
     9421 ggaccatccg gaatgcatac ggtgcggagc ctgcatcaag gcttgtccca aggatgcgat
     9481 ccactaccgg tttatgggaa aataatctta ttacgattac ttaaaggaga atataatcat
     9541 gaaaacaaac aaaaaattcc ttactatttt aacactctgc atagctttat tgcttacagc
     9601 ctgcggcgca acggatgctg ccggcacgga caaaaccaat gagtctctga cggaaaccgg
     9661 cacagtatcc gcagacagct ccaacacttc actggatgat ctgtatcagc aggagaacca
     9721 gatttttgca gatcatgagg ctgtatggaa caaggtattc ggcatgatga gcaaaagtaa
     9781 tgctgatccc agcgcaaatt atgctgatta tctggctgat accgtcgaag caaacaagga
     9841 ctcctttaca gaggatgaat tgaaaacact tacggacgat atggaaacca tacggaaaat
     9901 cgaagaacag ataacagaac tggaacaggg aactaccgca gccgatcata ctgctccgga
     9961 aaacaattcc gaagctgact ctcccttccg gaatttctcc ggtcaggatt ttgatggtaa
    10021 ttctgtcgat gaaagcctgt tttccgccaa tgcagtaacc gtcgtcaatt tctggtttac
    10081 cggctgtaag ccctgtgttg ctgagttgtc tatgctgaac gaattaaaca atacgctcaa
    10141 agcaatgggc ggcgaagtca tcggcatcaa tacggaaacc ttcgacggca atgaagctgc
    10201 catgaaggaa gctgccgctg ttcttgaaag ccagggtgca aagtaccgca atatttccat
    10261 tgactctgct tccgatgcgg gtaaatatgc ctctgatatt atggctttcc ctacaaccat
    10321 acttgtcgat agaaatggca atattgtagg ggatccgatc cttgggggaa ttgataacca
    10381 ggataactat gactctctga tgcagcagat tcaatcggta attgatgcag acagtgcaaa
    10441 ccaataatgc aaaacactcc ctacgaattc ttcattcacc tgttgacacc ggcaattgat
    10501 tttgatatta tttatcaaga aatgaatatt ccatgaggga gtagttagcg cgaaggtgtg
    10561 atttgtcaac attctgatta taattttaga gtataatctg gcaaatctta aataatgaga
    10621 ctcatgtata tctattaacg gtctgtgtat aatacacact ggcagataat tgatatacat
    10681 gagtcttttt catctctgtc gatacttatc atcgcacagc tttcggctga caacactata
    10741 atggatcacg caaaggagaa aaacatggag aaacttatta acaatgcccc gtttgccctt
    10801 gttcttgtat tccttgtaat cggttttgta ctgctgatta aaggtgcgga ctttttcgta
    10861 gaaggaagct ccagtgtagc gaagagactg catgttccgt ccatcatcat cggtcttacc
    10921 atcgtagcaa tgggaacctc tctccccgag acagcggtca gtgtatcggc ttctatcaca
    10981 ggcaacaatg agcttgcggt cagtaatgtc gtcggttcca atatctttaa tctgatggtt
    11041 gtgatcggcg tatgtgcaat gatcgccacc gtagaggttg cgcgggaaac tatccggaga
    11101 gatatccccc tttccctgat ctgcgccggt cttttactgc ttttgggaag catcggactt
    11161 ggtgatccgg ccaaaatgac actgggacat ttcgatggtg tgatattcat aggtcttttt
    11221 gccggatata tcttctacat gatcagaatt gctttaaagg caaataaaga aggaaaaaaa
    11281 gtagagattg agggcggttc cgatgaagag atcaagctga tctccgtacc gctcagcatt
    11341 gttttcattg tcggcggtgc tgctgcgatc gccttcggtg gggatctcac tgtcgatgct
    11401 gcatccagaa tcgcaagcga cctgggaatg acgcagacac tgatcggact taccatcgtt
    11461 tccatcggta catcccttcc ggagcttgtg acttccatcg ttgcagccag aaaaaatgaa
    11521 gtcgatatgg cactgggtaa cgccatcggt tccaacgtat ttaacatcct gatggtactg
    11581 ggtattgctt ccgccatcag tcccatcagc atcattacgg aaaacatcat tgatctgtgt
    11641 gtactgatcg catttactgt gtgtgtatgg atcttcgcag gaagcaaaaa gaaaatcgga
    11701 agagtcgaag gcttctgtat ggtggctctt tacgcagcat atgcggttta tatcataatc
    11761 agataaatga gggccgtaca aatgatattg ttttttaagg tgtttctcac ggaattcatt
    11821 gcggagatgg gcgacaagac acagcttatg ctgatcgccc ttacttcaaa atacaaatta
    11881 agagatatta tatttggaac cgctgccgca atcctggtac tgaacggtct ggctgtcctc
    11941 gccggcgggc tcgtcagcga attcattccc ggctggctta tcaagatcat tgctgcactt
    12001 gcatttcttt attttgccgc atcaacactt gcgggcgatg atgacgagga agaggaaggc
    12061 agcggcagat ccaggatccg gttcgcaccg cttgcggttt tctgtacctt tttcgtagct
    12121 gaactcggag acaagacaca gcttaccgcc atcacctttg gtgccaatga aggtatgagt
    12181 tccgcactga tcgtatggct cggctgttct ctgggactgt ttttagcaga catcctggga
    12241 atgcttttag gctatctgtt aaagagcaag acaccggaag ggctgctcaa tactctcgcc
    12301 ttcgccatct tctctgtatt cggcgtcttc acactgtatc agggactgaa gctcatcagt
    12361 acagatatct gttccatacc ggtactgccg atactgatcg tcgcaacgat tgcttttgcc
    12421 ggtatctgtg tgtacctgtt tttaaagaga gagaaaaagg caaaagcagc aaactaaaaa
    12481 cgaaccccat tctgttttca cggaatgggg tttatttatc tattccgttc ggaactcaaa
    12541 agaaaaatag tacacataat catccatttc aagggcatcc agctggtcgt tcacaatata
    12601 actaaaatca aaagttctcg cttcgtcatt atcatagatc agcgcaagaa gcggctgctg
    12661 ttcatcatag acaatatctg ttttctcctg aatttccgtt gcagctcttc catatccata
    12721 gtgctctcct tccggaacat cctccaggat tgggattagc atatctccct gcgattcatc
    12781 atctgcaata ataattttca cggtacgatc cttaaactgc ggtacaataa taatcgttcc
    12841 attctgcggt gaatccattc cttcatacga aagccctgcc ctttgtctgt ctaccagctt
    12901 tccttcccgg tattgccaaa tatgaatata tagagtttta aattcattcg ttgccgtgta
    12961 tttatacata aaggcaccgt ctacgcctga cagcatttcc gccgttttca tttcaatact
    13021 gtccttgtcc accggaacga taaagttctg tggcatattt ttccggaata tcatgattcc
    13081 gaccgtcaaa acaacaatcg ccgccgtgac aagcagaata cctataatgt atttcaatcg
    13141 tttttgcttc tgcttactgt ccgttgccag cccgataata ttttcttccg attttttggc
    13201 aatattttcc cggacaatat cttctcctgc caggaattca ttgatgctga taccgaggat
    13261 ctggcagagt tccgaataga cggatacatc cggcagacac acgccccgct cccacttgga
    13321 tacggatttg tcactcatac ccagtttttc ggcgagctgc ttctgcgtca tacctaagtc
    13381 ttttcttttg cctgcaatat attttcctat cttgacgaga tccatgtgct tacctcctgt
    13441 gtctgagatc atactattct cttttcagaa ataagtacac ctcccggaag tagaatcccg
    13501 gtatctactg tgttttccat tgtatactct tctattacac ctatttctgt tccaacaatt
    13561 tcttccagtc atccggaaaa cccatcgttt tcacatctac tttttcatat ttatctatta
    13621 gcaattctat ttgatctaca taaagattcc aatgcttgtc attctttaaa atctgtttca
    13681 tacacaataa tacaccaaac attctattgt ttcctatacc ggcttgtgta tattctttgt
    13741 aaagaatagg cgtctttgaa agttttgcat tatacaacct cccataatgc gcacaaacat
    13801 tgcgaacata agaaatgctc tccaaccaac tttccagata tgtatatcca attccaaaac
    13861 tcttggcaac tgcctttttg tcagcatttt tcatgttttt gtaaaatttt gaaagagtgc
    13921 caaaactaaa tacctccacc aacgcatata taggcaaatt tccacctgca taattagttt
    13981 tgaaatttct cacaaacggc gcctttgagt ttctccgaac ctcctcttcg atatcagcca
    14041 taaatgcccg atgatattct tcgtccacaa aattttgggg ttccatatat ccaagaactc
    14101 cataaacttc cgcaaaataa tttgcaattc tgcatctgac atttacttct attttttcta
    14161 tctcagcaaa tgtcacttgt ctgaaattag cattgaacag atataactct acaatctgct
    14221 caaatgttac gccttcttca tatttcccat ttttaggttt aaatcctaaa ctatacgcct
    14281 taattaatcg gaaataggat atgtcattta atatcttctt ggcatattcc tcattctcaa
    14341 taattaagtc aagtgatttc aagttttcta tttgttcgtc tattgtcatc ggtaatggat
    14401 gttgctttag ttcccccata tattttcccc cttaaaatta aaagacccaa cgtggtccgc
    14461 atcgttgaga ggcgtgttgg gttctgttat ttttagtata ttctatatgt tgtaaaaata
    14521 caatgattat ttcaaattac ttaaactttt tcttataccc gcagtcgcaa taagatccgc
    14581 cggaagccag ggtgtattcc ctgacaaaat tggtgacacc gcccgcctcg ctcatggtgt
    14641 agtcaaactg gcacatggca gggatcagct cagtaagtcc cagttccttc ataagggtac
    14701 agattccgtc ttgctgtgct tgtgtctctt gcgcacttct ctggcgtgat ctctgcgggc
    14761 ggcgatagct tctgcctctt ccttaggtat cagcttcgtg gtcttgacaa tatatacacc
    14821 gccgtcatat gccttgcgta cccggatctt gcccttgagg tagccgtgct tctcgcaata
    14881 agccacgcag taataatgcc tgccgttagc gctgaaccat ttgatcttct tgcgcaggtt
    14941 acgatggcag agataacatt tgctggacac cacttcctta tctgcgaagg cttctgtctt
    15001 gtccttgaat tccctggaaa tatattttac ataggtatca aactgcgctt tcacctcatc
    15061 cccacggtcc ttcggaggac agaaggtatc gtaggacagg ttcaccacta cctccgggtg
    15121 ctcctccagt atccggatca ggatctttgc cgtatagtag gcatcactga aggcccggtg
    15181 gaagggaata tccttctcga tatgcagaaa atctatggca tattccagat ttctgcggat
    15241 cttgcgctcc tcataggcaa tggcgaacag cttctgcaca tccagataag gcagcggtcc
    15301 cggtgccaat agcggcatct cataatatgc catattccgc tgtaactccg tcagatccag
    15361 agttccccag gtacagaaca gatgctcgtc ctgtccgcac cagttcacga aatctccccc
    15421 gacttccggg aaaggccgtc cacgctccag ctcctgcatc tgaatatgga tcagtttgct
    15481 ggtgatcttg tgcatctcat ggtagacctg cggtttgacc agttcgttga attcactgac
    15541 cataacacgt tctttattca gtttgatggc accgatctct atgatttcaa aagggagctt
    15601 ctccacagca tcctctttac cggtgttact ctgattccat tccagatcca gaacgatata
    15661 ttccatacgt tcctccatat ttttaactat taacaggggt tataaatgtc ttacagtctt
    15721 ggcagtgcga tatccaatac ctcttcatgc cagcagtggt ctccctctct gacatcgtgg
    15781 atcgggtatt cctcttttcc gtacaggctg taagccttcc gcacaatatc cagctgctcc
    15841 gtcacattca ccagtcctct ggggccgttc agatggtcat cccggcagga ctggaccgcc
    15901 agcggtctcg gagcaatgag cgatgcaata tctcccatat caaagtgttc ccacagatgg
    15961 ggaacataat tacagctgca atttccgttg agcaataaca acgcgtcacg atatccatag
    16021 agatatccgc tgatgataca ttttttcaca cggtcatcca aagcagagac ccacagcgtc
    16081 tgcataccac cgccggaaaa tcccatgact cccaagtcct cagtatccca ttcgccacgt
    16141 tcatatacat aatcgatcag ccgggaagca tcccaggtac acatacctgc cacggtctct
    16201 cccaaaggtt cagccatatg tgccagatga aaacaggtac tggtcaggaa ggacttctca
    16261 tccgttcctt tctgcaatgc ctcatccctg cgttctccga agcctctgca gtccggacag
    16321 attgccacat aaccacgctt cgccagctgc ataccgtaat cataatggta gaattcgatc
    16381 atccgtttta ccgcgggaat atcattgcgt cctgccacgc tgaatttacc tgcgccctga
    16441 tgccccggaa gggcaagaaa acatttctgc ttcccttcgt tctgcttcgg cgggatcagt
    16501 atatacatag gcatatacac ttccggctct acctgaagga tcactttttc ccgacgaatg
    16561 ccgttctcca gttccacgac ttcttccacg cgggggtcca gatcacaggt ctccatatat
    16621 ttccatccca gaagctcttt caaagtctct ctggaagtct ctttccaact ttcccattcc
    16681 tctctggtgg atccggtgaa agcatcctgt cttgcatagt tatcgaattt cttctgcatt
    16741 cccttcaggc ttccgtagaa gtgagggatc tgaatgatcg tatctgcctg ttccatgact
    16801 gctccttgcg ttttcatttt ctcttattgc tattcttacc attctctgcc ttgtgcgtgc
    16861 agtccgtctt ccacatctcc tgcaaaaagg ctctccagcc aggtatgcgc cagttcaacc
    16921 caggaggtgc attcctcctg tacggctctg ccatcgttag tctctgtcag cttattcgcc
    16981 agtgccagcc cgtgcgctcc cttttcatac atatggaatt ccgtgggcac tccggctctg
    17041 cggagagcgc tgacaaacag cagggaattt tccaccggta ccgactgatc cgcaaaggta
    17101 tgccagatga aggtcttcgg cactttattt gttacctgtt tctccaggga cagcttctct
    17161 cccagctcct cctgtctgct gcctaacaga ttctcaaaag atcctctgtg ggcaaactct
    17221 ccggaagtga tgaccggata gcataacagc agtccgtccg gacgcagcag ctcatgctcg
    17281 gatgccgaaa gtccctgact ctctgccaga aaatcttcat cccagaacac cccaaggctg
    17341 gctgccaggt gtcctcccgc agagcagcct tccactacga tcttgtgggg atcaatgtgc
    17401 cactctttcg catgctcccg caggaacttg atgctgtaag ccagttcctt taaggaagta
    17461 ggatatttcg caggggcgca ggaatagcgc agcactgccg catgatatcc catcgccagg
    17521 aatttcagtg ccataggctc cgcctctctg tcggaggttc ttgtatagcc gcctcccggg
    17581 cacagcagga tcagaggtct gtcgttaatg gccattgcct ccgaatactc ctgaatatag
    17641 gtaactaaat gtgctcccgg cagggaaccc tcctgatgca attcaaatgt attatagatc
    17701 atctttttcc tcatttccga gcattatcat tgaaaaataa ggcgcagacc ctgtagcaat
    17761 actaccggcc tgcgtcttca tctgccttat aaatattttc ataactattc agaaccccat
    17821 tcgcaaaacg ctcctatgga gctttttttt tgctcatgtg gttacttcgc cctataaata
    17881 tcataaaata ttcttacgaa tgccagcatt cgacgaatat ttgacgatat ttacatggct
    17941 ctgaatagtt acatattatc ttagtatgct cttccccagt ataccatctt tttcgcaggt
    18001 tttccacaac atacgcaggt atctgcgatc tgttcctgtt caaaaggcat gcagcggctg
    18061 gtaacagcaa gcttttcctt gatcttgtct tcacattcct ggcatccgca ccacatagcc
    18121 ttgacaaatc cggacttctc attgaagatc tgctcgaact cttccatgtt atgtgccaca
    18181 taagtatggc tgtcgcggtg tgctcttgct ctctccagca tttccttctg catggtctcc
    18241 aggatctcgg ctgccttggt ctccagatcc tccagagcta cctcggtctt ctctctggta
    18301 tcacggcggc agatcacaca cttgcctgcc tcgatatcct taggcccgat ctccacacga
    18361 atcggataac ctctcatctc agcctctgcg aacttgaagc cgggggtctt gtcactgtca
    18421 tctaccttga cacggaaatt cttcgccagg cggtctctca gctcatacgc cttgtcgaga
    18481 acgccttcct ttttctgcat aacgggtaca atgcagatct ggatgggtgc cacacgggga
    18541 gggatcacca gaccttcgtt atcaccgtgt accatgatca gtgctccgat cagacgggtg
    18601 gtcatacccc aggaggtctg atgtacatat tttaaagtat tgtccttgtc tgcgaactgg
    18661 atgccgaaag ccttggcgaa gccgtcaccg aaattatggc tggtaccgga ctgcagtgcc
    18721 ttgccgtcat gcatcagtgc ttctaccgta taggttgcaa ttgcacctgc gaatttctcc
    18781 ttctcggtct tctgaccctt gattgcgggg atagccagaa cctcctccag gaacttcgca
    18841 tatacattca gcatctgcag agttctctcc tgagattctt ctgcggtagc atgtgcggta
    18901 tgtccttcct gccacaggaa ctctctggaa cgcaggaaag gtctggtctc cttctcccaa
    18961 cgtaccacgg aacaccactg attgtatacc ttaggcaggt ctctgtagga atggatgtca
    19021 ttcttataga aatcacagaa cagggtctct gaagtaggtc tgacacagag tctctcagga
    19081 agctcattca atcctccctg ggtaacccat gctacttcgg gtgcgaagcc ttctacatga
    19141 tccttctcct tgtccaacag ggactcagga atgaacatgg gaagatatac attctgtact
    19201 cctactgctt tgaatcgtgc atccagctcc ttctggatca gctcccagat cgcatagcct
    19261 gcgggcttaa ttaccataca tccttttacg ctggtgtaat cacacagctc tgctttcttc
    19321 actacatcgg tataccactg ggcgaaatct tcttccatgg aagtgatcgc ttctaccatt
    19381 ttcttgtctg ccattttctc tctccttttc tttttctgaa aataaactgc tcatacgatc
    19441 tacggaaacg aaaaacgccg ggtcatcgct cccgaaaaag ggaccgatca ctcggcggta
    19501 ccaccctgat tgatacaaat atgtatcctg ctctgtcacc gttaacgccg gttctacgct
    19561 gtgcttcgcc gcttccaggt acattgtgtc tcgcacagga ctccaaggga ggtttccgat
    19621 cctctgtcat aaggggttcc cagccgcacg tcaccgtgtc cttcccctct ctctgggatg
    19681 ctttccgatc gtactgatcc ttttcatcgt cattgtcgta tgcaagtaat tgtagtgcat
    19741 acttccgggc tttgtcaagt tctatctgct aataagaaga tacctgccgg agctgcataa
    19801 aataaaagca gataataacg caccagggat atggggccca gaaagaccgt aaggcacagt
    19861 gccagaacca gcagccagct gttccatatc tctgccctcc gccgccggat accgtagaaa
    19921 aacaggatca ctgccagcca gaaataggtg ccgattgcaa acaacgcacg gatcaccgga
    19981 attttctggt aataatattc cagcgatatc ttacgataaa attctgtcag ccgggaactg
    20041 atcgtcttca tttccagtac atttcttccc tcgaaatcaa agtaataaat atcaccgtcc
    20101 ggatcatcca caaccgatgt ccccggatac caggcctgat acgtattatc cagaaaggct
    20161 ctcagataac atcccggata ctgccatcct tttcggaacc atagtctcag atactcccac
    20221 ttattttgaa gaagttcttt attattcaca taatttttga tccggtctgc cagaaacgga
    20281 ttatattgtg accactgttc tctgtccatg atctgatcca acagctgcaa ttcctcactg
    20341 tcaaacgccg cttctccctt atctgtatat acccttgcaa tctgctggag cggtacacac
    20401 agagcctcta ccgcggaacc tttttccgca tggagcagag tttccatttc cacagacaat
    20461 agcatggtca gcagaatcgt tcccgcaaac agtgaaatga tctttctttt ctgcttttta
    20521 cagaaaaccg cgcacaacac cagataaaga aaaaccgcat aaattacatt tttccgatac
    20581 agcgctccca cgatcaggct gcacgataat gctgcgattt tctctttctt ccgaaaaaat
    20641 ccctccgtat tctcatacaa ttcatacaac aggtgaaatg caataaaaag tgccagagag
    20701 cataccacat ccttggtcgt actgacggaa aacaaggcaa tcgtcggcag aaaagcataa
    20761 aaacaaaatc cgactcccaa cagccatttt ctattcaatc gccgatacca gaaatccaga
    20821 ctgtaggaaa aggtcgccgc acaaagtatc atctggaagc aggagtgaag aaatactgcc
    20881 ttctccatcg tcccgaagat ccggtatcct aatgtgataa cgcctcccat gaccaacgtg
    20941 ctgagtaacg aatggtgtgt attatacggc acctccggat acatcacctg aggcacctgc
    21001 ccgtaaatat cataattata aaatccggga tcattggaaa ggagcaccgg cagccaggct
    21061 atcaacaatc ctgcaaaaat caaaaatatt ctctttccga tacctttccc gataccgtcc
    21121 tcctgaactg ccttgtctct ccctgcatgc acaaacagcc atcgcagcat ctgtccaaaa
    21181 agtaatgtct ctgctatcca ccacaaaatc cagcctgcca tagggacctg ttccagccac
    21241 gactgcatcg ttctatgtac ggaagtatgg tattctatac tgatccggca tcccactgtc
    21301 tgacaggttg ccagtaatat ctcaggtatt cctacagcaa atctgtcagt ccagtccaag
    21361 tctctccctg atgctgtccg ctgcaatatt ccagtcaccc atttcttcat atgcggcatc
    21421 catcttctcc ttttctctgg cagctcgctc cgtatctccg gtctgaagaa atgccttata
    21481 catggacgcc gcctcaaaat atctcgccac accatagcat tcctttttcg ttccggacag
    21541 caagccgccg tacaccatat cctcatgata atttactact aaataagcat aattttcact
    21601 ctgtacatta ttctccatcc agttggtctt cgcaggacgg gaaaaggcgt catttaattg
    21661 ccggaacata tgaatggttc cccaaaacaa tatcaggcac agggctatga tcaccagatt
    21721 catcacaagc agcgcgcctc tctgcttctg tgcctgtttc agcttactct tcagatttac
    21781 cataacccat cacctcgcct ttttcttcca ggaataagat tttttgtgca tttgacatcg
    21841 taccgaaatt ctctgcctct tccggggtca tgccaaaata ggtctgcagc agtgcctttt
    21901 cattctcttc catccggaga atggcttcct tctgctcctc cggcagttcc cgatattgtt
    21961 ttgctctata atcattatcc acaggatgca gaatcctttc cagctgctcg taataatccg
    22021 aaatattttc atacagaccg ggaagtatgg atacattgcc ggagttgatc gtaactgcct
    22081 tcatcagact ttcataaaag taacgatacg ctccgctggc ttccatgagc agctgatatt
    22141 tttcataatt attattactg ctataaggcc ggatgtaatg cctgtcgcaa aaagctgata
    22201 aacctatgta atcctcatct tccagatatt gttccatctg tttcatatag ctgctctgcc
    22261 ggatccctgc atcaaactgc agcaagcctc tgtggatgct ccaactacgt gcagatacta
    22321 caaacaccac cacacacagg atcaccagca ctgtgatgat tatcatatgt gacatgatct
    22381 ccgtattttt ctccgccact tcatagacat cacttttggt atcctcaaag ttgctcttgt
    22441 aatgctccat gtctttgaca tgctgtgcgg cttcttcatt cggtcttccg caatgcggac
    22501 aatatgcctg ctccaatgtc agattgcccc cgcaatattt acatttcatc attgctttta
    22561 tgatcctttc ctattcgtta tgcttcttaa tgtattcctc gcaatcctca tatctgggat
    22621 aaccgtaatt atttcgcaga ctatcctcga attttttctc ttcttctgcg gtaaacgtat
    22681 aacggtctgc cagactgttc agggttgctt ccacataagg acggatatac tccctgtctt
    22741 tctccgtcag aatgacctcg ctgtagtcca gtccccgcat catccaataa tcatccagca
    22801 gcaatgcctg ctgatactca ttcaaagtct ctccactctg ctccagtgcc agatattccc
    22861 tcgcatcccg acagctcatg agccagcgga aaataccact gtgttcccat cggtctatgg
    22921 ttctgttctc ttcgacagcc tgctcataaa aatccatcag tgcttccaga tccttctcct
    22981 gatagagacg gtccagtatc ggaaagttct ctttttccca cagataatcc gctctggcat
    23041 ccctgggttc tatatactgc acccggaaca caattaccgc aagtatcgct gccaatgccg
    23101 ccaataagat cagaagctta atcacaaatt tctgtttctt tttcagtttt ttcttcagtt
    23161 ccttctccgg tacctgctcc aactgctgca tatcctgccg catgctttcc agcttcccca
    23221 gatattccgc ttcggcgccc ttgtaattca ggcttccgca atagggacac ttcggaaggg
    23281 tatcctcaaa ttctgcccca caggaactgc agatgatctt tttaactgtg gtatctgcca
    23341 tacaaatcct catatctttc cacaacctgc acactttgga ctgccggtgc aaagctgctt
    23401 attgttcaca atcattatta atatacaaca caaaaatgac acgtgcaatg cagaatcact
    23461 ttttgcacac atcttgaggt ttttgtctct ttcctgtaaa ataaactcag tcgaaaaacc
    23521 tgcccgcagt tcaaaattgc gggttgctga aaggaatgac gatcgatatg tatgagaacg
    23581 gttttctgga tccggcctat atcacaccgg aggaacaaca gattttagac ggtaatattg
    23641 ccgttcagaa gaatctctac accagcaaac aggattttgt agtagagagt gataaatttt
    23701 tggaacactc cgaagatgat attactctgc gtcgccacac ccgctttgtg gattttccaa
    23761 agcataaaca tgattacgtg gagatctttt attgtctctc cggttctgtc acacatgtca
    23821 taaatgaaga agagatctgt ctccgccccg gggaacttct atttatgaac caacatatcg
    23881 aacatgcggt aatagcctgc ggccaggaag atctgggcat taacatcatc atccgccccg
    23941 cctatttcca gaatatcctg actttgatca acaaggataa tattctggcg gattttatca
    24001 tcaatgcgct ggaaggtgac agttgtgtcg gtcagtatct gtactttacc gtctccgata
    24061 atccctgcat ccagaacctc atccacaatg cggtcttcct tctgttacag cagcctcaga
    24121 actggcataa gctctcggaa cacacctttg ccctgctgtt catgcatatc ttaagcaatg
    24181 cggaaaatat tttattagac cagaaagatg agcagagcaa tgtcctgatg gtggtggtac
    24241 gcagatacct ggcggagcat tatgataccg gaactcttca cgaactggct gcacagatcg
    24301 ggtacacccc atcttccctg agccgtatga tcaaaaaata ttccggcact acctttaagg
    24361 aaatacaaac acggcagcgt atgcgggtcg ctatgaatca gctgtcccac accgcgctgc
    24421 cggtgtctga gattgccctg tctgtaggct atgagaatca gaacttcttc tacaaacagt
    24481 tcaaaaaaat gtatgatatg acacctaacg aggcgcgttt aaaatcgcgc cgataacgtc
    24541 aggcattcaa gatataacgg ttcgaggctc cttcgtgtgt ccctcggatc gttatttttc
    24601 atataagaat tcttccggaa gggtcacacc gaagtccttt gccagatggc gaaaatcatc
    24661 gggggtaatg gtctgtctct tattcggctg catggacaac atcttcacca gaatttctgc
    24721 ggatttttct acggtatgca tcagaccgaa ggtcaggtcg aagtctttgc ctgcggcaaa
    24781 ggttccatga tgtgcccaga tggccagatc gtatttcttc atcagttcgg aggtagctac
    24841 tgcgatctct ctgccgcccg gtaccatcca gggcactacg cccacaccat ccgggaatac
    24901 tacggggcac tcggttgcca tctcccacag ttctctggtg aataccttat cctccagcgg
    24961 aagcacgaag gtcagcgcaa tgatattggt ggtatgtgca tgatatacca cacggtagtc
    25021 gggatcctgc agcttcttca cttccagatt catcaggtgg ctggggagct cggaggtagg
    25081 tctgcccccg tttaccagac cccatacgat acggtaattc tcacccttgt tgtcaagttc
    25141 gatcatgcag atagaatctt ccggcttaat gctgacatta cgaaaatatt tgccactgcc
    25201 ggtcaccagg aaatactctc ctgccagtgc gggaactgtg gtgccgatgg gctggaactc
    25261 cttcggctca aagttctcct ttaccagttc tacctcttcc ggcttcacac ggtaagacag
    25321 gttgccgccg ttacgttcat gccagccttg ctgccaaccg tcatctgcca ttctgataaa
    25381 tccctgtaca aattctgcat ctaaaaattt catgataata cctaaccttt ccatcgtctg
    25441 caacctcccg gccgcagaca tatttttcaa aattataagc attttctaca caaatgctat
    25501 tatctggtaa tgatatctac cggcacattt aatattttag caaccttcag gatcgtgtca
    25561 atatgtctgc cggcagctgc tgccatatga tgggtagggc ctgcctcgct ccaacggttc
    25621 acgaactctc tgcaggggat tgagaatttg gtacgcatat tggtgtcacc gaaagtgaag
    25681 atcgggccgg gctgattctc gccttctgcc acaacgaact tgaagttgcc gtccttatcc
    25741 tgcgtgatag ccagataggt caccgggcct acgggaggat agaactgagt cagatatccg
    25801 ccgcctacct tgccgtggaa taccttaacg atcttcatgg tgggcttatg tgccagagag
    25861 atatccgcat cgccggaccc ggaatgaccg atgatgcaga tatcctcgtt gaaatcaata
    25921 gagtacatct cggacaactg tcccataccg gagatcgtct tcagaatgga cattgccatg
    25981 gcaactttca tatcaccttc tacggcacat gcggtacctt gcttgatcag catggaaaat
    26041 gcagggatca gcatggaatc cagtttaccg gctactccct gtgccttacc ggcataatgg
    26101 gaggcgatca tacttaagtc ttcatccttc gcccactgct cgaaagcaag cacatgcttt
    26161 gccatatcca gaatatcttc gtcatctgca gtagcgccac cctctacttc gaaggtatcg
    26221 atgatgtcct gtgccttcgc cttaatagct tcggtatcgg taatatcgtc tgcaatagcc
    26281 cacatatctt cccagtcaaa ctgcttggta tacaggccca ttctgttgta aaggttggtc
    26341 tcatcgatat agagatccat cattccggga taagggcggc cgatctgggc aatattggtc
    26401 tgacggaaac gtctgcggac ctgagctgcc ttacaccaat cttcgatctg agctgccacc
    26461 tcggggtcgc cgccttctac cacgccagtg attacagcat gtctcttgcc tgcacgctcc
    26521 aggtctgcca cagcctcacc tacggcacca caggcataca ggtcacccag ccagatggga
    26581 atatcggtat tggcatagtc cggcgccagc ttcttctgca cattgataca tactacaggc
    26641 acatccagat cacggatcgc gggaagtacg ttataggaag ttgcataggt cagcaactgc
    26701 aggaatacca gatctacgtc tgcagcacgg aatttatcac ctgctgccat agcctgttcc
    26761 ttggtggtca ccatgccacc atcaacgata tctacagtat cgggaatcgt cttcttgaaa
    26821 tcctcatact gtgcctcgaa attaggcacc agctggggga actggggcag atatgctccc
    26881 agagcgatgg aaaatacacc gatctttgcc tttgtgttta ctaacattta taatttcctc
    26941 cttttcttaa ggtcacgttt taagtaaccg ctttgcctgc gcattcgcaa gccgcttttt
    27001 attactttgc cggagcaaat gtcttaattt caaaagactc ccggacacat tttctggcac
    27061 tcttcagatc tgcaagatct cctgcatgga tcatctgcgc caataggttg ccgatagcgg
    27121 ttgcctcacc gggacccgca tatacggtct tgcccgtagc atccgctgtc aactggttca
    27181 gatatgctgc attggaaccg cccccgatga tatggatgct gtcataggtc ttcccggcga
    27241 tcttctccag acctcttacg gtctctccgt aggactgtgc gagactgcgg tatactacgg
    27301 aagtcagctc tcccacggac tccggtaccg gttgtcctgt cttatgacag tactgtctga
    27361 tggcttccac catgctgtcc ggagagagaa aactctggtc attcacatcc actctggaag
    27421 ggaactcctt gctttcctcc gccatattgc acagctgtgc aaaagagtat gcatcgttat
    27481 attcgtgtct tacggactgg atcatccaca gtcccataat atttttcagg aaacggaacc
    27541 ggtgatcgta accgccctcg ttggtaaagt tggccgctgc ggcatcctct gtaatgaccg
    27601 gtttcaggga ttcgattccc atgagagacc aggttcccga gctgatataa atgccgtctc
    27661 cttctgtctg aggaactgcc attacggctg atgcggtgtc atggcttccg cacagtacca
    27721 cttccagatc gaatcctact tccttctgaa tctccggaag cagctggccc actttcgttc
    27781 cgggcatctg cagcggcagg aaaatatcct ccgggtaacc cagcttacgg atcagctcct
    27841 tatcccactg gaaggtatcg ggattcacca gctgtgtcgt ggaaccgttc gtgtactccg
    27901 acaatttatt gcccgtcaga cggaaaccga agtaatccgg cagcatgaga agtgtctttg
    27961 cctgtttcat aatctccggg gtcttctttt ccactgccat aagctgataa atcgtattaa
    28021 aaatctgctt ctgaataccg gttctggcat acagttccgt ctccggcagt attttcgcca
    28081 cttccgcatc cattccgtca gtacggtgat ctcggtagcc gtaagtatct cccagaatct
    28141 gatctttttc atccagcaat acataatcca cagcccaggt gtcgattgcc atgctgaccg
    28201 gaagcttgcc cagttctttg cattttttca ttccgttcag aatctcgccg aacagacggt
    28261 tcacatccca cagaagcttt ccgtccttat gatccatgcc attctcaaaa cgatagatct
    28321 cttccagctc tattttgccg ttctccatgt gtcccaaaat atgtcgtcca ctggaagcac
    28381 cgatatccac ggccagataa tatttttcca taagatatcc acagcctccc ttatctgaaa
    28441 ctgaatacct gtgtaagaag cggctgtgct cctgtaacag gatccttctc catgatcaga
    28501 atatctttca tatattcatc ccattttttc actacatcgc tctctgcctg cacttttgcc
    28561 gcatagtcga cgcccttttc acattcgtaa taaccgaaca gttcctcgcc ctgcatccag
    28621 atagagtaat ttacaattcc agcgtttgcc agaacttcct tcatctccgg ccagatgttg
    28681 tcatgtctct tcttatattc ctcctgcatt cccggtacaa tttttccgcg ccatgcgtat
    28741 ttttccatac aaaccaccat acctttctca ctcattattt tcgttgcaaa tctgctctgt
    28801 tacccaatct ctgtcgcaat attcctttgc ttcgggcatc agctttttca taatctgtgc
    28861 ttgcagaagc acaaacttgt gcttttaacg atattataga aaacataatc tgcttctgaa
    28921 atgacattat ttgctccctg gactgtccga ttttgcccga tccgtacgat aactgtttat
    28981 tacagcacat gaattgtgtt atgatacaat aagttcaccc gtataaagca gccgccgtgg
    29041 ataaattgta ccgcaaattt gatcacgata gccgcccaag caaagagagt atgaaaaata
    29101 ccatcgttat gtatcataaa taataattta gaaagcacaa tttttatgga acgtacatta
    29161 acctatacaa cagatcattt catatccccc ctgccggtca gccatttcct gaagcagaaa
    29221 gggttttctt cccagaatct ggtgcagtta aaaaaagacc ccaccgccgt gcaggcaaac
    29281 gggattccct gcttcatgaa tcatatattg cagccgggag ataccttgac gctgcatatc
    29341 cacgaggaac actcctcgga aaaaatccca ccggtggagc tgccgctgga tatcatctac
    29401 gaggacgagg atctcatggt gatcaataag ccggcaggca tgccgatcca cccttccatg
    29461 aacaattatt acaattccct ggcaaacagt cttgcctatt attttgcaca gcagaactgt
    29521 ccttttgtgt tccgctgtat taatcgtctg gaccgggata cctccggact gaccatcgta
    29581 gccaagcatt atgtcagcgc cggaatgctg tccaccatga tcgccaacaa ggcaagttcc
    29641 ggtatcacac gggaatacct tgccattgta aaaggctctg tgctgccttc tgggggtacg
    29701 attaccgcac ctttagggcg aaaagaaggt tctatcattg agcgttgtgt ggattttgaa
    29761 aaaggggaat ccgctgttac ccattaccgg gttttggacg aaaaaaacgg gcacagcctg
    29821 gtctctctca ttttagagac cggccgcacc catcagatac ggattcatat gaaatacctg
    29881 ggttatcccc tgatcggaga ttatctgtac aatcccgata tggcattgat acagcgtcag
    29941 gcactgcatg cctggaagct gtcctttgtc catcccatca ccggggagaa aatgcagttc
    30001 acagcacctc tgccggagga tatggcgaag gtagcgggag ataccccatg gagcatatcc
    30061 tgaatctgct taatctacaa gcagtcatgc agattcctat ggtgcagtat tatgcacgca
    30121 gtttcttcag tgctttctcc cggatcaggc attcaccgtg ttcttccaga tatcttatct
    30181 tctgctcgtc tgcggcatac aacgctgcga attccatagc ctggatgcag gctctgctgt
    30241 agcgctcgta ggacgcaccg cccttctgca catagacata gcaagtgccg tcttccatcc
    30301 ggtacaacgt gctgttgatc cgcaggttcg ccggtaatac ggcggcatat tcgatcactc
    30361 tgccgatatt gcggaaggca aaaattgcaa actggggctt tgccgcttcc tctgcttttc
    30421 cctttgcttt cttatttttc ccggcattgt tctccgtttc ttccttcgcc tgttttccca
    30481 gcgtagcgcc gatctctgcc tttttctcct cgaagacacg cttcatct
//
LOCUS       DAWDHD010000011        30477 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_125_length_30477_cov_13.392479, whole genome shotgun sequence.
ACCESSION   DAWDHD010000011 DAWDHD010000000
VERSION     DAWDHD010000011.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 30477)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 30477)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..30477
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125_length_30477_cov_13.392479"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         8901 a         6339 c         7950 g         7287 t
ORIGIN      
        1 catccagggc tgtattcttc tgaacgacca gattcatatt ttcccggaaa agttcgactt
       61 cattctcaaa atcttcttta aacagatgaa attccggaga acttttgatc cgttcatagt
      121 gggaggattc cgtttcagaa agatccgatg ccgggagtac atcttcggcg aagattgtga
      181 gaataccgta cagatccagc ttcgtgatga gcttgtccgt gagaaccgtg cccttcggaa
      241 tcagaagttc atgatccaga ttgtagacat cttctgcaac aatcattccg ggaatcaatt
      301 gtgtgatgga taatctcttc atagaccgta atccttgtgc ctttcggtaa cctgaaaatc
      361 atcaggtgct ttcgtaaatc ttacttattt caattataat aatttttccc cggactgtca
      421 attttccggg acagaataga tgaagagagc tgttcataaa atttttacag tcttttcacg
      481 gctctgcagg cacagaaagg ttgaaacata gaggaaaatc tgctacaata acctttgaat
      541 tatgcgaaga cccatggaca ttttattgtc aaaagggtgc ggaaacgagg aaaaaatgag
      601 cgttattgag aagatatttg gcacgcacag ttccagagaa ctgaagcgta ttgaacctct
      661 ggtagacaag atcgaggcac tccgccctgc gatgcaggca ctttccgatg aggagctgcg
      721 ggggaagacc gaagagtaca agaagcgtct gaccgaggga gagactctgg acgatctgct
      781 tccggaggct tatgcaaccg tgagagaggc tgcaaagcgt gttctgaaca tggaacacta
      841 cagagtacag ctgatcggtg gcatcatcct gcatcaggga cgtatcgcag agatgcgtac
      901 cggtgaaggt aagactttgg tgtctaccct tcccgcttat ctgaatgctt tggaaggcaa
      961 gggtgtccat gttgttaccg tcaatgacta tctggcgaag cgtgatgcgg agtggatggg
     1021 tcaggtacat gagttcctgg gactgaaggt cggcgtggta ctgaacagca tgacctccga
     1081 ggagagacag gaagcttaca agtgtgatat cacttatgtt accaacaacg aactgggatt
     1141 tgattatctg cgtgataata tggttattta caaggaacag ctggttctgc gtggattgca
     1201 ttatgccatc atcgatgagg tggactctgt actgatcgat gaagcgagaa ctcctctgat
     1261 catttccgga cagagcggca agtctaccgc tttatatgaa atgtgtgatc tgctggcacg
     1321 tcagatgaag cgcggtgatg atgtacagga actgaccaag atggacgcca ttatgggtgt
     1381 ggtacaggaa gagaccggag acttcgtagt caacgagaag gataagatca ttaacctgac
     1441 cgcagcaggt atggagaagg ttgagagatt cttccacatt gataacttcg ctgatcccga
     1501 gaacctggag atccagcaca acatcattct ggcattacgt gcccataatc tgatgttccg
     1561 tgataaggat tatgtggtaa aggatgacca ggtactgatc gtcgatgaat ttaccggacg
     1621 tatcatgccg ggacgccgtt actccgacgg tctgcatcag gcaatcgaag caaaagagca
     1681 tgtaaaggtg aagagagaaa gtaagacgct ggcatccatt accttccaga atttctttaa
     1741 cttattcgag aaaaagtgtg gtatgaccgg tacggctctc actgaggaga ccgagttccg
     1801 tgagatctac ggcatggatg ttgttgtgat ccctacgaac cgtcccgtta tccgtaagga
     1861 tctgcaggat gctgtataca agacgaagag agagaagctg aacgctatcg tgaatgcggt
     1921 agaggaagca cacgccacag gacagcctgt actggtaggt acgatcacta tcgaagccag
     1981 tgaggaactg agcagaatgc tgaccaagtg cggcatccag cacaaggtat tgaatgcaaa
     2041 attccatgaa ctggaagcag agatcgtagc cgatgccggt gtacacggtg cggtaaccat
     2101 cgctaccaac atggcaggcc gtggtacgga tatcaagctg gatgatgaag cgagagccgc
     2161 cggcggactg aagatcatcg gtaccgagcg tcatgagtcc agacgtattg ataatcagct
     2221 gcgtggtcgt tccggacgtc agggagatcc cggtgagtcc aagttctaca tctctctgga
     2281 ggacgatctg atgagactgt tcggttccga gcgcctgatc agcatgttca ataccttagg
     2341 catccctgag ggacaggaga tcgaacacaa agcattgacc aatgccatcg aatctgcaca
     2401 gaagaagatc gagagcaaca actacggtat ccgtaagaat ctgttagagt atgaccgcgt
     2461 catgagtgaa cagagagaga ttatctacga ggagcgtctg cgggtactga acggcgagag
     2521 catgcgtgat gtgatctaca agatgatcac cgatattacc gagaactgtg tagatatctg
     2581 catcaatgac gacgcagata tctccgactg ggatttcaat gagttaaata ccctgctcat
     2641 tcctacgatc ccgttacagc ctctgacacc ggagcgtgtg aagaagccca agaagaacag
     2701 cttaaagcag cagttaaagg aagaagctgt gaagctgtac gaagcaaaag aagccgagtt
     2761 ccccgaaccg gaagccatcc gtgagctgga gcgtgtggtt ctgctgaagg ttattgaccg
     2821 taagtggatg gatcacatcg acgacatgga gcagctgcgt cagggcgtgg gcttacaggc
     2881 atatggtcag agagatcctc tggtagagta caagatgagc ggttacgaga tgttcgatga
     2941 gatgactcag aacatccggg aagagactgt gagacttctg ttccatatca agatcgaaca
     3001 gaaggtagag cgtgagcagc aggcgaagat caccggaacg aacaaggatg attccttgcc
     3061 gaagggacct gtaaagcgtg aaaatgcaaa ggtatatccc aatgatccct gtccttgcgg
     3121 cagcggcaaa aaatacaaga actgctgcgg acggaaagcc tgatgctttc tgacaacagt
     3181 cggaagatag tgcggcagca cggaacaatc ctacccaagg tctttcaccc acctatgagc
     3241 aagctcatgt gggcttagac cttttgatcc tggcatcatt aaaataatag aataagaaag
     3301 tgggtgacgt taagtggttg aattagatca gatgaagaca gaaatcctgt cctacgaaac
     3361 acctttagcg gaagtgaggg attcactttg accttgctaa caaggcaaag cggatcgaag
     3421 aaatggaacg tgagatggag gctcccggat tctgggatga cccggagcgt tccaatcaga
     3481 aaatgaaaga actgaagaac ctgaaggata ctgtgggaga gtgcgacaag ctttcgacac
     3541 agttcgatga catcctgacc ttgatcgata tgggatatga ggaaaacgat gcgtctctga
     3601 tccctgagat ccgtgaggag ctggatgaat tcatcaggga attcgacgaa ctcaggatcg
     3661 gtacattatt atccggagaa tatgacaaaa acaatgcgat cttaaagctc aatgccggtg
     3721 ccggaggtac cgagagctgt gactggtgcg gcatgctgta tcgtatgtat acccgctggg
     3781 cggagcgcaa gggctttgcc atcgaagtac tggactatct ggatggtgac gaagcaggta
     3841 tcaagtcagt gacttttcag gtcaacggcc tgaatgccta cggctacctg aaatccgaga
     3901 agggagtaca ccgtctggtg cgtatttccc ctttcaatgc tcagggaaaa cgtcagactt
     3961 cctttgtatc gctggatgtt atgccggata tcgagggcga tctggatgtg gatattgatg
     4021 agaaggatct gcgcatcgac acctaccgaa gcagcggtgc cggtggccag catatcaaca
     4081 agacctcctc tgccattcgt atcacccata ttcctacggg aaccgtggta caatgtcaga
     4141 atgagcgaag ccagttccag aacaaggata aggcaatgca gatgctgaaa gccaagctgt
     4201 atctgttaaa gcaggaagca aatgccgaga agctgtctga tatccgcggt gaggtcaagg
     4261 agattggctg gggtaaccag atccgctcct atgtattaca gccttacaca ctggtcaagg
     4321 atcatcgtac cgatgtggag accggtaatg tggacgcagt actggacggc ggactggata
     4381 tcttcatcaa tggctatctg aaatggatca gcgtgcaggg agataagaag aacacagaga
     4441 attaacaaat tcaagtttgt aaatgacaca ttccagaagg agtgaaagct ttctggaatg
     4501 tgatttttta taagggagaa aattacatat gagaaacgaa aacaaagaga caaaccaggg
     4561 caaaaaagcg ataggagagt ccatgggcag cagaattgtg cttgccctga tcgttgtggc
     4621 tgtgatagtt acggcggcaa aagtgatccc tttggtccgg aatcgggtac agacttccca
     4681 atatccggtg atcgagctgg cagatctgca gggaatggaa ctggagagct taagtgatgg
     4741 aaagttcagt ttcagctatc cggcagaaga ctgggaagtg tgggagcagg tgacgacgga
     4801 ttatcacccg cttacgatct tctataaagg gacagcggat gacggctcca caagtatctc
     4861 ggtggcggag aatgacaccg tggtcacaca gttgaaaccg gaacttctgc aggtaatgtt
     4921 ggatggctta aaagaacagg ctccttatat ggtgataaag gaaagtgaaa tgcgcagtat
     4981 ggaaggtaca ccggtgttct atctggagga caatatgtca ctgacccagg aaggactgga
     5041 tatgatggta gataccggcg cgtggacaga ggaaacgata gaagccatgg gcggccgtga
     5101 ggcactttta agtaacaata ttgcttcgga ccagattcag atctatcagt tgaaaaatgg
     5161 aaaaatttat gtgtataccg gaaattatac ggatgatgcg cagaaagata tggtactgga
     5221 caccatcacg gtgatagcac agacaataaa gagcacaggc aattaatgcc tgtgctcttt
     5281 ctgcatcaga tccagaaaat ataaggtctg ggtcatgttc atatatggca ccagccacag
     5341 agttccgata ccgaagctca gcaaacacag aaatcccagc ggcaggaaac tcatttgtaa
     5401 gcagaacagc tcccatttat gtcctttcat aatacggaag cttaatttca tcagctccat
     5461 agcgctatac tggggaaaat ccaacagtaa atagtaagcc tgggataaac caagggataa
     5521 cggaatatag atacacatac cgatgaccat aagcacaatg cacaggattg ccatggttat
     5581 ggcgtcaaag cccttccctg accgcaggag aaaatagcag atatcaaacg gaagtgtaca
     5641 tgccgtattc agcaggatca gtactgcgga gatcccgagc gattttttga ataagtattt
     5701 gaagccgtag aacagattgg cagttacagc aggtctgctg caggcagtat tcagataaaa
     5761 taatgtgatg ccggtctgaa atactccggt gaaaatcgac aataaagtcg tacaggctgt
     5821 caaaagcaga tattccgcca gagatgtctc tgcgcttccc cccataagtg tccgtacggt
     5881 aaggatcata gtgaaggtca tgctgacggc aaaggtgagg gaaaatacga tcagactccg
     5941 gcagatggac agcaggatgg cattgccgta atgccctgcc atctgctctc tggctttttc
     6001 tttgagttgg gctctggatg catattgtat catagaaatc ctcttttctt tttattattg
     6061 cgttctattg gttattccgg ctgctccggg ttttgatttc gtaactgcag aatgcatcgg
     6121 aaaacatagg catgtcttac aggtaagatt gccagaattc ttccatatcg acaccgtaca
     6181 tctgctggta catctgatcc aacatattca tataagaagg atctgtgaat accgtgacaa
     6241 tgctgctgac cgttatgaag ataccgagtg ccagtcccac acaggacagc cagatcccgg
     6301 caacacacat attgttcatg cgttgccctc ttttccgggt gagcaatgca aagagaatgc
     6361 ccagagcacc ggcgggaatg gacagcagtc ccgtgcagca taataccagg gaacagatgc
     6421 cgcagaccat agaagcgatc gacaaacccc tgtattttgg atcgatctgt ggtatatttt
     6481 gattcatttc gttattctga ttctgattat aatccataga aagcctcatt tccgggcgac
     6541 atatggtgaa tttgtctgcc gcgcttatat taagtactgt accatctttt tctgtaataa
     6601 gcaaatgaaa taagaatgaa aagattgaga aaacttccga tttccagtat aatataccca
     6661 tgcgcaaaac aaaatggcaa caggccatca ggaggaacta tggatattat acagaaaatc
     6721 aaagaagaac ttcaggtaga aaaatggcag gtggaagctg cggtgaagct gatcgatgag
     6781 ggaaatacca tccctttcat ctcccgttac cgtaaggagg tcaccggatc tctgaacgac
     6841 gagcagctgc gtaatctcga tgagagactg acctatctga gaagtctgga agataaaaag
     6901 gagcaggtgc tgaagagcat cgaggaacag ggaaaactta ccgatgagtt aaaggagaag
     6961 attctggcag cccagacgct ggtagtggta gaggatctgt accgccccta tcgtcccaag
     7021 agaaagaccc gtgccagtat tgcaaaggag aagggtctgg aacctctggc agaatatatt
     7081 ctgcgccagg aggcaacaga accggtgctt aacgaggcag caaagtatgt ttccgaagaa
     7141 aaagaagtaa agacgccgga ggaagcattg cagggagcac aggatattat tgccgagatg
     7201 atctccgacg atgcggatca tcgactttac atccgcaata tcaccgtaga ggaaggcatt
     7261 gtgaccggta cggcgaagga tgagaaggct cagagtgtct acgagatgta ttacaatttc
     7321 gaagagcctg tgaagaagat cgcaggtcac agagtattgg cattgaaccg tggagaggca
     7381 gaaaaagtac tgactgttaa agtcaatgca ccggaggaac gtattctgcg ttatctggag
     7441 aaaaagctga tcacgaagga aaatgaatat actacaccgg taatctgtgc cgcagcggaa
     7501 gacagctacg atcgtttaat agcacccgct attgaaagag agatccgtaa cgatctgaca
     7561 gaaaaggcag aggatggagc gatcaatgtc ttcggtaaaa atttagagca gctgttgctg
     7621 cagcctccta tcgcaggcaa agtggtctta ggctgggatc cggcattccg taccggatgt
     7681 aagctggcgg tagtggatgc taccggaaaa gtgttggata cgaaggtaat ctatcctacc
     7741 gcaccgcaga ataaggtgga ggagtccaaa gctgaactga agaaactgat caaaaaatat
     7801 aatgtggatt taatttccgt aggaaacggt acagcctcca gagagtccga gcaggtaatc
     7861 gtagaactgt tgaaggaact ggatcgtccg gtacagtatg tgatcgtcaa cgaggcaggt
     7921 gcttccgtat actccgcaag taagctggcg acagaagagt ttcccaattt tgacgtggga
     7981 cagcgaagtg ctgcatccat cgccagaaga ttacaggatc ccctggcaga gctggtgaag
     8041 atcgatccca agtccatcgg tgtcggccag tatcagcacg acatgaacca aaagaaatta
     8101 agcgatgcct taagcggcgt ggtggaggac agtgtaaaca aggtcggtgt ggatctgaat
     8161 actgcgtccg catcccttct ggaatatgtc tccggcatta acaagactat tgcgaagaat
     8221 attgtagatt accgagaaaa taacgggcgt tttgtaagca gaaagcagtt gttaaaggtg
     8281 ccgaagctgg gacctaaggc atatgagcag tgtgccggtt tcttacggat cccggatggt
     8341 aagaatcctc tggatgccac cagcgttcat cccgaatcct acgaagcagc ggagcagctg
     8401 atggcgaagc tgggactgac aatggaggac attaaggaag cccagaaaca ggcggcagct
     8461 aagaaagctt ccggcaagag cggggcacag gcttccggac agaagaacgg cagtaatgca
     8521 gccaatacag caaagaaacg ggaagagcag aaaggtaaga ccgtaagagt gcataatacc
     8581 aataccgcta tgggcaaggc tctggcagcg gccatgggtg gtgtgagctt tgataattct
     8641 gcagaaactt ctacaaagaa aaatgcaccg gcagtacaga atgcatcaaa tgatgcaaaa
     8701 gatatttccg gactggaaaa gcgtattaag aataagaaac tgctggcaga agagctggga
     8761 atcggcgaga tcacgctgac agatatcctg aaggaactgg agaagccggg cagagatccc
     8821 agagacgata tgccgaagcc gatcctgcgc agtgatgttc tggatatgaa ggatttaaag
     8881 cccggcatga ttctgaaagg aaccgtgcgt aatgtcattg actttggtgt gttcgtggat
     8941 atcggtgtcc atcaggacgg actggtgcac atctcccaga tcaccgaccg ctatatcaag
     9001 catccgctgg aagctgtcag cgtcggagat attgtggatg tgcaggtatt gactgtggat
     9061 gtggcgaaga aacgtatcgg tctgaccatg aagatccaga agcagtaatg ttatgacccg
     9121 ccccgatatg agcataaaag tgggatgata atcccacgat atgcgaatag agggtagggt
     9181 gctatgtgcc agtggcacat gtttagcacg gaccgaagcg gagcggagaa gtgggagtgc
     9241 gtagcacgga acaatccgca ggcatcaaag tcgggggctt ttgactccaa catcgtatta
     9301 tcataagaag aaagagggat aacacagcaa aatgttattc ctctttcttc ttatgatttt
     9361 ataactaata acaccaaacg atacgaaaag tgaaatagat ctttattatg ccttatgacc
     9421 tgttttgata atagggctta aaggcttgta gatgagcagt gtcacaacag agaccagcgt
     9481 tcccttgatg agattcatgg gagccacaca tgccaccacg aagcttacaa tgctgccttc
     9541 ctttgccagc ggattgactt cggtacccat tgccaggatg gcatccagag gcattccgta
     9601 gaacttggag aaggcgggaa gcagatatac ggcattgaat gcggtaccga atatcgtcat
     9661 gaccagtgta ccggcaatgc aggcaaggat ggcgtttttc ttagttttct taaaggaata
     9721 cacagcggaa gcagggatga tcaggctgca gcccactacg aaattcgcca gatcgccgat
     9781 gaaagcggta gaggtacctt ttatgcacag cttcagcagt atcttcacga attccatcat
     9841 gacacctgct acgggaccga aagcaaagga accgactaag atcggcagct cactgaagtc
     9901 cattttatag aagccggggg caaaaaagat ggggaagtcc agcacatgca ggatcatggc
     9961 gatggcggag aacataccga tcatggcaag ctttctggtg ttgaagatcg gctcagaaac
    10021 tccgtttttc ttctgggcac tcttttccag caccacagcc accaggaaac accccacgat
    10081 gaccgctagg aagatgacta cataggccac attttcagtt acagtttgca ttaatttgtt
    10141 catttcagaa ctcctttcct atgagaaaat caaggaaagc cttcaaaaaa agccccggaa
    10201 tacaagcttc cggggcatag gtatcacaaa aaaatataag tgatacgtct tctttcatcc
    10261 agactgtact gttggttccg gattcacacc ggatcagccg cagaatgcat ctgcgggtcg
    10321 cggacttaag gatgacctat taccgccagt agggaattgc acccaaccct gaagaacttt
    10381 accttatgca tggaatataa cactttgtga acatattgtc aatatagaaa atctttagaa
    10441 ttttcccaac atttttttcg gatttccctg ttatagtagc tgtaataact attcagaacc
    10501 gcatgcgcaa aatcgccctt ccagagcttt ccttttgcat gactctgaat agttgcaaat
    10561 acttatagaa atgtgttgac actcaataga tattattgta ttatgcaaac agtacagtga
    10621 cacaatgaag aatacacaga aaatgtatca tgaaatggga gtatgtgagg aatgagtgca
    10681 ttagtagaga tcaaagatgt gtgcaaggtt tacaatcctg gggaaaatga ggtgcgggca
    10741 ttggatcatg tgagcctgac catcgacgaa caggaatttg tggccatcat cggacattcc
    10801 ggaagcggta agtccacgct gatgaatatg ctgggatgcc tggatgtgcc caccagtgga
    10861 gaatattggc tccatggaca ggatgtatcc gcattgacag acgatgaact gtcggatatc
    10921 cgcaaccggg agatcggctt tattttccag ggattcaatc tgatccccaa tctgactgcg
    10981 gtggaaaatg tagaactgcc actgatctac cggggagtat ccagaagtgt acggcaggag
    11041 ctttccagag aagcactgaa aaaggtggga ctggaacacc gtatggatca taagccttcg
    11101 gagatgtcgg gaggacaaca gcagagagtg gccattgccc gagctattgc ccaggcacca
    11161 ccggtgatcc tggcggatga acctaccggc aatctggact ccaattccac aaaagagatc
    11221 atggatattc tgaaaggttt acataatgag ggaagaacag tcatcctgat cacccatgac
    11281 aatgatattg cggcacaggc aaaacgggtg atcaagatca aggacggcaa agtggaatcc
    11341 gattccggac agatcgttcc ggtggaggag ctgcagacag caggagcgta gtggaaaata
    11401 aatctttcac atgcaatgcc atgccgccgg tcacggctaa caaattatga aaagataaga
    11461 gtatgaatga atatagaaag gatcataagt gagggatcat ggaacagcag gaaaaaacag
    11521 aacaggcact gagtgctaaa aaagtcaagg aaatgaagaa agcagaaaag agaaagcaaa
    11581 aagaagcaaa aaaggaacag aaggcaaaga acccaggcaa aaagcgtaaa aaagcacctg
    11641 ttattattgc ggctatcata attgtactga tcgtgatacg catggtaagc tgtgcgtttt
    11701 ccggcaatgc tggagtgatg gtaagcacca ctaatgcatt tcgcggagac atcgaagaaa
    11761 atgtcagcac cagcggaaaa gtagccagtg aggaaaagac cgttttattt gctccggtca
    11821 gcggaagatt aagcgagatc aatgtggcag ccggagatgc cgtcaaggca ggagatgtcc
    11881 tgatgaccta cgacatggat cagatggaac agcgcctgca ggaggcgtcc ttacagcagg
    11941 ataagagtaa tgcttcttat aacagtacta tgacagagaa cagcaaaagt acagcaaaac
    12001 tgaatgaggc caataccaat ctagcagtgt tggatcagca gcttaccgat tacaaggcat
    12061 atctcaagga actgcaggat aaactgagta cgagccagag ggagacccag agacagttgt
    12121 ctgaggagag ctacgatctg agcagaaaag cggcagacct aaacaaagaa ctgcaggacg
    12181 gtgtgacagc agaacgggcc aaggaaatca ataaggaatt acaggatatc agcagcagtc
    12241 aggccagaaa ttcttacgtg cagtctattg ctaattccag tgattatgta gtgagtatgc
    12301 agaatgagat cgctaccgtg caggagcata ttacggaatg tgaaaattac aaggccgaga
    12361 tgcagagcca gaagggcagc agtgagggaa cgatcctgaa tggctatcag tcgcaagcct
    12421 atgcggcaga taaggatctg gcacagttga cctatcagga ggcacaggaa cagtacgatg
    12481 cggcgaaaaa aggaattgtt gcggactttg acggtattgt aacggagtgt accggtgtct
    12541 ccggagccag cgtaacagaa ggagcacagc tgatcacact ggagagcagc cagaatgtga
    12601 aagtttcctt tgatgcttcc aagtccgatg tggcaaaact tgccatcggg cagaaagtag
    12661 atattaccat ctccggcaat aaatacgaag gcgagatcag taagatcaat cgtatggcaa
    12721 cactgaacgc atccaatacg cccatggtag gggtagaagt acatctgact aacccggatg
    12781 acaagatcat cctcggactg gatgctaagc tgaccgtaca taccaacagt gcacaggata
    12841 cattgttgat cccggtagaa gcgatcaatg cggataaaaa tggagatttc ctctatgtga
    12901 tcgagaacgg catggtagtc aagagggcaa ttacctgcgg aatctcctcc gatgaataca
    12961 cagaggtcct ggatggcatt accgaggaag accagatcat agtgaacagc ctcacgggta
    13021 tcagcctgga ggagggcatg gcagtaacgg ctgtgcccat ggaacagtag gaggcggatc
    13081 catgacacaa gtatgggaat acatcaaaat tgcattgatg aatatccgca gtaataaggg
    13141 acgttcgatc ctgaccatgc tgggcattat cattggtatt tcctccgtca tcatgatcat
    13201 ttccatcggt aacggcgtaa aaagtggtat caacggagaa ttgaattcca tggcgggcgg
    13261 ccagatcgca gtttatagca acgataccgc agaaaacggt acggaagtga tatttaccga
    13321 agaggatatg gatgccattc aggagaaagt tccaaatgtc aaggcggtaa caccgtcctg
    13381 gagtttcagc ggaagcgcca ccggcagaaa gggaactttt gatgctactg caactttcgg
    13441 taaggcggga ctggaatatt cgagccagga tccgatcatc aagggacgtt attttaccga
    13501 ttcggattat tatacggcga ataaggtctg tgtcattaca gagagcagcg cgaaaacgct
    13561 gtttggtaat accaatgtga tcggaatgag ttttgattat acgttgtatg gagtgacaca
    13621 ggaatttacg atcgttggta tccgtaagga caacgcatcg aagctgttcg gaatgggagg
    13681 aaacggtacg gtgaccatgg aggcacctat ctccacaatt tctgaggggt atggattcta
    13741 tgtggattac acggacctgc tgatcgtcag tgatggcgcg gaaaatgcct ctcaggtggc
    13801 gcaggatacg gtgcgtctgc tagagaacag gcatggtgtc agaggacaga atgtcatctc
    13861 tgtacagaac tttaatgata ttatgagcca gatggatcag atcttaagct atatcactat
    13921 ctttgtagtg ttcgtagcag ccatctccct gctggtaggt ggtatcggcg ttatgaacat
    13981 catgctggta tctgtgacag agcgtaccag agagatcggt attcggaaat ctctgggagc
    14041 caggaccgga tccatcctgt tacagtttct gtcggaatcc gccatcatta ccctgctggg
    14101 cggcctgatc ggtattctga tcggtgtggc aggagccttc ggaatctgca gcctgattgg
    14161 atttaccgca aaggtgagtg tgggtacggt gctgggagcc agtatgtttt cctcggctgt
    14221 aggtattttc tttggaattt atccggcgaa aaaggcagcg aaattaagcc ctatcgaagc
    14281 attacggcat gaataatatg cgataaaagt taaatcataa ttaaataaaa tgcaaactat
    14341 aagaaccgca ggtcacagat attggaatcc ctgcggttct ttacttgctt ttttatggga
    14401 aaaaatttat aatgttctct atagtatgca atcctatgcg cagtcgccgg atggtgggga
    14461 tgggattgta atagcaagta actatgcagt cacattatta taaggaaaga actcattatg
    14521 gtagaattgg atgtgaagat tacatcgggg gatttatatg actttttatt gcggcacagc
    14581 tacaacagtg tgtccggact tctgggagcg gtgattggag ctttgggcgt gatcgtgggc
    14641 atctcgagac agtactggat ctatctgatt cttggcgtgg tgattctgtt atatctccca
    14701 tggacactct atatcaagag caaacagcag gcagtaaaca atccggtctt caaggaaccg
    14761 ctgcattatg tgttggatga gaacggcatt accatctccc agggggacag ctccacctgt
    14821 cagaaatggg aggattgctt gaaagcggta tccaccggac gcagcatcat tgtgtatacg
    14881 gcgaaaaata atgcaacaat ttttcctaaa agtcagatgc aggataagac aacggacgtt
    14941 attgaaatga tttccaccca tatgccgccc acaaaagtga agatccgcag ctagatgcat
    15001 ggatacagca gaagaataga gcatgtggtt acttcgccat ataaaactgc ttatctgtct
    15061 gtatgcaggc attcgcagaa cgcagttttg catggctccg aatagttacg aattatataa
    15121 aaagtaacag taactgcata accgtaacac gtaacatgta acaatagaaa gcgtaaggaa
    15181 aatgacaaca ggcgattcag cagataacag agaatgcaaa aaagagatca tagagaccca
    15241 cagtgcggaa gagacctttg cactgggaca gcggatcggc agggaagcac ttcccggaca
    15301 ggtctacacg ctgatcggtg atctgggagt aggcaagacc gtatttaccc aaggtgtggc
    15361 agatggcctc ggaatcacgg agccggtgaa cagcccgacc tttaccattg tgcagatcta
    15421 cgaggaagga cgtatgccct tttaccattt tgatgtctat cgtattggcg atgttgagga
    15481 gatggaggag attggctatg aggattgttt ttacggggaa ggactttgcc tgatcgagtg
    15541 ggcgaatctg atcgaagaga tcctgccgga acattacagg caggtgacca tagaaaagga
    15601 tctggagaaa ggctttgatt accgccggat cacgattgag gagaggacta cagtatgaaa
    15661 atattagctt tggacagttc cggactggtg gccagcgccg ccatcgtgga ggatgacgtc
    15721 ctgatcgcgg aatacaccac ggattacaag aaaacacatt cccagacatt attacccatg
    15781 ctggatgaga tccggcagat gatcgatctg gatctgcata ccattgatgc gattgccatt
    15841 tccgcaggac ctggttcttt taccggactt cggatcgggg cggctacggc aaagggactg
    15901 ggactggcac tgaaccttcc gctggtagag gtcccgacac tggagggact ggcttttaac
    15961 ctctggggaa cggacaagct ggtatgtccc atcatggatg ccagaaggaa tcaggtatat
    16021 accggaattt atgagttctg taaagaggag atcccggaaa aggaaagctc agaacatcaa
    16081 cttgtgatgc acagtatcaa agagcagtgc gctattgccg tggacgagct ggtggaggaa
    16141 ttaaaccgtc tgggacggga agtgatcttc ttaggagacg gcgttccggt ctacagggaa
    16201 cagatcttac agaaactgaa ggttccctgc tcctttgctc cggctgcaaa taatcgtcag
    16261 cgtgctgcca gtgtggcttc tctgggagct gtctactatg cacatggcag aacggtgacc
    16321 gcagcggagc atgaacccga atacctgcgt aagagccagg cagagcggga acgagaggaa
    16381 caggagaagg ctgcaaaaga ggcacaggca tgaaaataga agttcgtaag ttacagaata
    16441 cggatatcga agaactgagc cggatcgaag ctgcgtcctt cagcatgcca tggttggcgg
    16501 aagatttcag aggacttctg acgagagatt attgcctgta tgtcgtggcg gaggcagacg
    16561 gtcatatcgc gggctgcgcc gggctgacgg acagttttca tgagggcaat atcgacaacg
    16621 tggtgacagc accggaatat cgaaatctgg gcattggcgg caaaatgctg gaagagttgt
    16681 tacgcctcgg caatgagcgc gggatcacag catatacgct ggaagtcaga gtgagcaatg
    16741 caccggccat ccacttgtat gagaaattcg gttttgtctc cgaggggatc cgcccgaaat
    16801 tttatgaaaa accgacagag gatgccatga tcatgtggcg ccgttaacaa gtattaccat
    16861 cacttttccg gcggattatc cattataatg atgccagggt caaaaggtaa catttacagt
    16921 cagaaaggtg gaaacaggat gcgaaaagaa gaaaacggta aattacagca ggtaatctgt
    16981 aacggctgcg gcaaggagtt gaaaatagaa aatgaaatcg tccgtgaagg atgtttcgcc
    17041 gcagatgtga gatttggcta tttcagcagg aaagacgggc tcagacacaa gtttgacctg
    17101 tgcgaagact gctatgacaa atggatccat acgtttgccg ttccggtgga agaaatgccg
    17161 gagacagagc ttttatagac agaaagaaca aaaagatgct gcttaggcaa gattgcgagg
    17221 aaagatgtta gacgtttgct tattgggaac cggaggaatg atgccactgc cttacagatg
    17281 gctgacctcc ctgatgatgc gctataacgg gaaaagtatt ctgatcgact gcggagaggg
    17341 cacccagatt gccatgaggg aaaaaggctg gagcccgaac cccatcgaca ttatctgctt
    17401 tacccatttt catgcggatc atatcagcgg tcttccggga atgcttctga ccatgggaaa
    17461 tgcagagcgc acggaaccgc tgctcctgat cgggcccagg ggacttgcca aagtggtggc
    17521 ttctctgcgg gtgatcgcac cggagcttcc ttttgaagta aaatgcatgg agatcacgga
    17581 gaatgcccag acattttctt ttgaaggatt ccgtctggaa gccttcaaag tgaaccacaa
    17641 tgtgctctgt tatggataca gtatggtgat tgaccgtgcg ggtcgatttg ataaggacag
    17701 agctttggag caggaaatcc ccatgaaatt ctggagccgt ctgcaaaagg gggagactct
    17761 ggaggaaaac ggaagagtct tcactccgga tatggtactg ggagcggaac gaaagggcct
    17821 gaaggtgacc tacagtacag acacccgtcc tactgaaagc atccgtcaga atgcccaggg
    17881 agcggatctg ctgattctgg aaggaatgta tggcgaaccc gacaagctgg tcaaagccag
    17941 agaacataag cacatgacaa tgtacgaagc ggcgaagatc gcaaaagaag tgggggcacc
    18001 gaaactgtgg ctgacacatt acagcccttc tatgacgaga cctgaggaat tcatggatga
    18061 tgtccggaag attttccccg cagcttatgc ggcaaaggac ggctggacca tggaattgaa
    18121 ctttgatgaa gggaagtagg aaaatatgaa aaaaaacaac acgtcgatga cgaaaattac
    18181 ggtttttctg atcttcctca ttgtcctggt agtgggatat tattcgtatc tgtcgggaaa
    18241 aagcaggaca gaacagcagg aggccattat gtcagaggtg gatacggcac tctccagaga
    18301 tctgaccaat aattatcctg cgacacctaa ggaagtaatc agatattaca atgatctgat
    18361 caaatgcttc tacaatgaag actgtaccac ggaggagctt caggaactgg gcaggaaatc
    18421 ccttcagctg ttcgatgagg aactccgggc aaataacgaa gaagatacct atctgacccg
    18481 attacaggga gaggttaaga actataagga caataagaga aaaataacaa gtgtttctct
    18541 tgcgtccagc accaatgtgg actattatac agcagacgga tattcttttg cccggattgc
    18601 ctgtggctat ggcatgacgg aaaacggaaa aaagagttcc accatgctgg tatatctgct
    18661 tcgcagggat gaaaaccgca aatggaggat ctacggctgg gagacggcgg atcagttgaa
    18721 tgatagtctg gaacagcagg agaataatta aaagcaaagt aggcagaaaa atggcagaga
    18781 cagaaaaaca aaaagatatt tatattctgg gaattgagag ctcctgtgat gagacagcgg
    18841 cttctgtggt gaaaaacggc agggaagtgc tttccaacgt catttcctca cagattgcat
    18901 tacataccct gtacggtggt gtggtgccgg aaatcgcatc ccgcaaacat atcgagaaaa
    18961 tcaatcaggt cattgagcag gcgcttgccg atgcccatgt gacgctggaa gacattaccg
    19021 ccattgcggt gacctatggt cccggattgg tgggagcact tctggtgggt gtatctgagg
    19081 caaaagcaat cagttttgct accggaattc ccctggttgg agtacatcat attgagggac
    19141 acatcagtgc caacttcatt gagaataaag aactggagcc tccttttatc tgtctggtgg
    19201 cgtccggcgg ccactcacat ctggtggtgg tcaaggatta cggtgaatat gagatcatcg
    19261 gacggaccag agatgatgcg gcgggagaag cttttgacaa ggtggctcgt gccatcggac
    19321 tgggatatcc cggcggtccc aagattgaca aggtgtcgaa ggagggcaat ccggatgcca
    19381 tccacttccc cagagcagca gtggcggaga atgaatatga tttcagcttc agtggtctga
    19441 aatccgcagt gctgaactat ctgaacggat gccagatgaa gggagaagag attaatcagg
    19501 cggatgtggc tgcttccttc cagaaagcag tcgtagatgt gctggtgagc cattccctcc
    19561 atgcagtgaa ggcttatgga ctgaataaat ttgccattgc cggcggcgtg gcttcgaatt
    19621 cttccctgcg ggcagcgttt gaggaagaat gcggaaaacg taacatagca ttttatcatc
    19681 cctcccccat tttctgtacc gataatgcgg cgatgatcgg tgtggccggc tattacgagt
    19741 acattaaggg tgtccgcagc ggttatgatc tgaatgcggt gccgaattta aagctgggcg
    19801 agcgataaga accgaaaaaa gacttcaagg aaagacagac acaatatggt agacagagta
    19861 gcacaggaca gaaatgagac agaagattac agcatgaaaa atgagatgga aaacggacag
    19921 gagcctcagg agaatgggga aaagcccaga tgtaccgcta ttatccttgc ggcgggcagc
    19981 ggaagccgca tgcagtcggc ggtggcgaag cagtttttac cgttaaaaga aaagccactg
    20041 atctggtatg ccttgcatgc ggtggaggaa tcccggatca ttgatgactg cattctggtg
    20101 acggcagagc aggatattcc ctatgtccgg gaagaaatcg tgcaaaaata tcattttacc
    20161 aaagtaaata ttattacttc tggaggagcg gaacggtatc tgtccgtggg gagagcctta
    20221 cagctgattg ctgacggacg gttgaaagta cccaatgagc acggatatgt gttcattcac
    20281 gatggagcca gaccttttct gacagagaag atattagaag atacctatgc agcggccgtg
    20341 caatatcagg cctgtgtggc ggcggtacag agcaaggata cggtaaagat cagtgacgga
    20401 gaaggctttg ccctgtccac tccggatcga agaaccgtct ggaatatcca gacaccgcag
    20461 gtatttgaga ccagcctgat cctgcaggca tacgagagac tgaaaaatcg tcttcccagg
    20521 atgcaggaac agggcattca ggtaacggat gatgccagcg tggtggaact gtttactgag
    20581 acaagagtga agctggtaga ggcttcttat cagaatatta agatcacaac gccggaagat
    20641 atgaaaacag ccgaggcatt tctggaggat taaatcctcc gaagatgtcc cggctgttcc
    20701 tttgtgcatt tctatcaaga aattgtttta tttttcccgc atggcactgg gggtcactcc
    20761 gtataccttc ttgaaggcac gccggaaagt attggaatta ttatatccca cagcaatata
    20821 cagctcattc agactgatat cggtctcctt gatcagtctt gcggcctccg tcatgcggat
    20881 attctccaga taagtggaga agttgactcc ggtcttttcc ttgaacatat gggagaaata
    20941 gctctctgag atctggaatt catcggagat cttgttaagc cctaaggaag ggtccttgta
    21001 attttcctta atatatttct caatggtggc ggataacgta ttgtcatcct ccttattgcc
    21061 gaagagatta cacagggata aggcaagatc ttcgcacaat ttgaaggtaa ggtgctcatt
    21121 gaaacgttca tccagtacct ttacagcttc gggatccaca tcctggggca gtgcaaatct
    21181 tgcttttaat aaagtgttac ggatatctga cagtaggtat tttaacaggt tgaccggcag
    21241 ggtacgctcc tcgatattct cctgatggat caggttgaac atctctaata cctgcggcgt
    21301 attgcccgtg gagatgaagt ggatcagctt cgtggagatc tccggcggat aatagaatac
    21361 gttggaatcc ttcttaatga attcatacgg gaagaaaata tagttcttcg tcgtataact
    21421 tacagcttcg gaggcctgct ggtagcattc ccatacattc atcagggaat ccgtattgcg
    21481 tccgataccg gcgaagagcc agatgtcgta agtatccagc agataatcat gaagtcgtaa
    21541 aatacaattg ttgatcttca ggatcagatc ctttccctct tcttcggaac agtgcagcag
    21601 aacggaatag gtccggtccg aagggctgaa gtagaagagc ggtgtaccga agaaattctc
    21661 cagtgcggac tcgatgatct tttccagttc ttccatggat tccacgccgc cggggagagt
    21721 gatttctgta ttgtctccga aattattata gtagacgatg tacaacacat tataataaga
    21781 agaatccgca atacccagat agttttccat gtaggtaact tcatcctcgg aagtagcagt
    21841 accggataac agctgtctca cataggagct acagatgatc ggacgctgac tgtctaccac
    21901 ttcctgcaga tttttctgta cttccacagc ggtctgcaat tcctgcccaa gctcaatgat
    21961 cgggcggaca ttgcggcgag agaaataata gaccagacag ccgcctgtaa gcaatgccag
    22021 cagggcaaaa aagatgaaaa gcagctgggt gccacccaat ccggcggccg ccttgtaggg
    22081 cgggaagctg aaatagtaag attaactggt gttatccgaa acgtatttcc ccaaagtaaa
    22141 gttctgtcca tcgatccgga cctctgcata tcccttatta aagtccagag aatccaacat
    22201 ttctgcggaa atgctgtccg gagtatttat cgtaagcacg gtggagtcat cttccgcggt
    22261 caccgccagg aaattatcac catccatctg aacacagtcg aacagatccg ccatcttatc
    22321 ctgttcgaag ataaagcaga ccttcgcatt ggcatccata taatagagat cgttcaggtc
    22381 gatgatatac atataatatt tttcgctgta atttggcatg aactgatcca tgggaagaaa
    22441 gcggttctcg tattccggtt ccgtcatgta ggagtgccac atttcctttt cggtagaagg
    22501 gtacttctgg atccagttgt agaaccgctt tgcggatata aaataagtag gattcaatac
    22561 ataatctgtc tccggaaaat agacaaagga ctcttttaac ggaagaagag cttctgcata
    22621 tacattggta gccagagagt tctgcaattc atttcccatt tccgtaaaag gataataggt
    22681 attctcataa ttcatgactt tccggaaagt gtcgttctgc agaagctgtc tgcaatagac
    22741 ttccatgata ttcagatcgc tttcaaacag ctctgcattg ctgatcaggg ttgtctcgtt
    22801 ctgctgatca taagaatcac gcgcgttatt tatcgtagaa cggtataaaa agatgaacaa
    22861 aaccagaatg atcagcagga aaatactgta agaggttacc attttcagca ttaatgtatt
    22921 gttgtggctt gtataatttg tatcattaag ggtgtctttt ttattaaaaa tcattcatga
    22981 tcactcccgt gtctcaattt ttttagataa tataccgaat aaaccatgtc tattgtatca
    23041 gttacttaca aaggaagcaa gatgtaaccc ctttcaaatt taaaaatatc aaatgtaagt
    23101 actttcaaag aatgcagtaa aatcaagggt ttgcagaatc ggtggtcaaa atttgcaccc
    23161 gaacgcaaaa attgtaactt gtttttcccg gtgacggatg gtaaggtgag ggcaagtcaa
    23221 gggaatgacc ccatgacaaa tcatgattcc aaatttttag ggaggaacaa aatgaagaga
    23281 aaagtattat ccgtactttt agcatctgct atggtagctt ccttagcagc atgcggaagc
    23341 agcaatgatg ctcctgcagc atccaccgac agcgcagcaa ctggcagcgc agcagcttct
    23401 accgaggcag catctaccga agcttacaat ctggaggaga ttaacgtagt agttaatggt
    23461 actctgaccg caaccgttga caacggccag gctgaattcg ttcagcagtg ggatgatgca
    23521 gtatccgaag caatcggtca tcctattaag atgaacgtcc agcagttaga ccattccggt
    23581 tataccgatg cagttggtcg tctgttcgca ggcggtgact atcctgatgt aatgatcatg
    23641 agtgcagata tgttcaagca gtatgcacct accggtctgc tgtgggatat gtccgaagca
    23701 tatgcaaacg caaaattcca gtctcacctg atcctgcctg agatcaatga gaacctgaag
    23761 gatgaggaag gtcatctgta tggtttcgct cctacctatg gtaacggatg tgttacctat
    23821 gttaagcagg catggttgga tgcagttggc ctgaaggctg aagacatcaa gacttatgat
    23881 gattactata acatgctgtt aaagttccac aacgaagatc ccgatggcaa cggcgtgacc
    23941 ggtgatacct atggtgttat cgcagctggt ttcgttggta acgaggctcc ttatgtaaac
    24001 tatctgcctg agttctggca ggattcttat cccgcaatcc tgcaggatga gaatggtacc
    24061 tggtacgatg gattccagac cgatgctacc aaggcagctc tgttaagatt acagcaggct
    24121 tacaaggatg gcgctatcga ccctgagacc ctgaccgctt ctaccaagat cgcccgtgag
    24181 aaatggttct ccaacgatca gaccggttct tcaggtgtat tcacttactg ggcaggttcc
    24241 tggtatcaga ctctgaccga caacctgatc aagaacaacg tagatgataa gctggttgag
    24301 ctggctccca tcgctgaggt tggcgcatac ctgaacagag aagctcctgt atgggttatc
    24361 attgatgacg gtgacggaga caactcccgt gagcaggcta tcttcgatgc attcatcgag
    24421 accatgatgg atggcgacaa ggtgcagact ctgtggacct acggtgcaga ggatgttcac
    24481 tggtctacca aggctgaaag cttcaccatc aatgcaggta ctgacaaaga gaagtcctac
    24541 gagtacgcag acggtcagtt ccacttaaag cagtctccta acgatcctaa ctccgtatgg
    24601 aagaagaacg ctatggatcc cgctctggtt atctgctctc tggacaacgg ctttaacgat
    24661 atctccagcc tggctgcaga aggcaacaag ttcttcaccg agaactgcaa ggatgctcct
    24721 cagtctccta cctctgagac ctacaccaac gagtccggta ccatctatga tgctaagctg
    24781 gcagctatca cttctgtagt agttgacggc ggtgatgtag atgcagctat gcagacctac
    24841 gttgacactg ttggcggtat cattgatcag tgcttagctg aactgaatca gtaattgaga
    24901 ccgtaatata gtagcaaaag ctgcaaaagc atgatataat tatcctgtcc catcgggata
    24961 caaagcctgt gggacaggat attttagaga gaaaagaatg tgtgagatga agttgaaagg
    25021 aagagggatg gtatgagcaa gaaaaagaaa atcaccacat ccaccggcgt agaagtacgt
    25081 cacaagcctt tgggtctccg gatctggaat aacagaggct attatgtaat gttcctgcct
    25141 gtatttatat ttattttgat catgtattac tggcccatgc ttggtattcg ttatgcattc
    25201 tacgattaca agcccgtagg tgctcctact tttgtgggac tcaagcactt ttacagtatg
    25261 tttaagaccg atgcgttctg gagtgcattc aggaacacat tggaactgag tatcatgaag
    25321 ttattgatca cgaccttcac tgcggttatc gtatcaatct tcttaaatga aatgggaaat
    25381 ctctttgcaa agaaaacttt gcagactatt atatatctgc cccactttat gtcatgggca
    25441 gtagttgcat ctatcttcag cctgttcctg tctccctcct ctaccggtct ggtaaacgga
    25501 atgctgcgtg actggggcat cctggcaccg gatgccaagg gaatttattt cctggcaagc
    25561 actgcatggt ggagaccgat cttctacatt atcaatatct ggaaagagac cggttgggga
    25621 accattatct tcctggctac tctggcaggt atcaacccgg agctgtatga agcagctgat
    25681 atcgacggtg caacccgtat gcagaagatg cgtttcgtaa cgctgcctgc actgtccaat
    25741 actattatca ctgtattgat cctgaacctt gctaaggtta tgaatatgtt cgagtccgta
    25801 ttcgtattgt acaacaatgc ggtatacagc gtatctgatg ttatttctac ttatatttat
    25861 cgtcagacat tcgcaatcgc tctgccgaac tatggttact ctacagcagt tggtctcttc
    25921 aagtccctgg ttggctgtgt actggtactg atatgtaact gggctagtaa gaagacccgt
    25981 ggccgtggca ttgtatagga ggtgttaaac gtggcaaaga cagcttatcg taaacctaag
    26041 aaaaaggttc atgtattcga tattgtaaac tacatcgtat ttattctgat cggcctcctg
    26101 gttctggtac ctatctggaa ggtagtagta gactccttca atgctgtggg tgtataccag
    26161 ttcagactgt ggccggagga ttttacctta gatggctata agaccatcat tcatacccag
    26221 agactgtaca gacctttcat caactccgtt atcactacgg tgcttggtac tttccttggt
    26281 ctgctcctgt ccaccctggg cggttatgta ctgatccagt ttgatatgcc cggacgtaac
    26341 ttcttcgcat atttccttct gtttaccatg atcttctccg gtggtatgat ccccgagtac
    26401 ttggtaatga agcagttaca cctggttaac agcatgtgga tcctggttgt gaagcatggt
    26461 atgaacgtgt acaatctggt gctgatgcgt aacttctttg aaggaatacc tgccagcctg
    26521 ttcgaggcgg ccgagatcga cggatgttcc cctatgggaa tcttcttcaa gatcgtgctt
    26581 cctctgtcca aagcagctct ggcttccatc ggcctgatgt tcgcagtaac cgtatggaat
    26641 gactattcta ccgttaagat ctatattacc aatcctgatc aggttaactt ccagtatcag
    26701 ttacgtaata tgatcatgga tggtgatacc cctactacgc agtattcagt atcccagaac
    26761 actctgttct atgcagcgat cgtatgtgca atccttcctt tcatggcatt gtatcctttc
    26821 ctgcagaagt atttcgtaaa gggtgttaat atcggtgcgg ttaaggagtg attttcttct
    26881 cacacgtatt tagacttaga caagggaaac ttcggtttcc cttgtgctgt atctgtgagt
    26941 aaattccaca agaaaagatg cgcaggggaa gcgcagaaat gaggcagaaa tgaataaaat
    27001 tttttgggct gctgattcca cagtccagac caatgatttc acaacatatc cacagacggg
    27061 cattggccag gtatttcctt tatttgttaa aaatgagtat cagatagtaa accatgccaa
    27121 gaacggcaga agcaccaaaa gctttatgga cgaggggaga ctgaaagcca tagaggagaa
    27181 aattggtgca ggagatttcc tgtttatcca gtttggacac aatgacgaga aaaaagagga
    27241 tcccaccaga tatacagagc ctttttccac ttacatggag aatcttgaaa cctttatccg
    27301 tgtggcaaaa gatcatagcg cttatccggt gctgatcact cccttagagc gcagatgctt
    27361 tatggatgag aaacacttgg ggatcggagc acattccgat tatgtggctg ctatgaagca
    27421 gaccgcggag aaaaataatg ttcctctggt ggatctctac agcatgagcc gtatggagct
    27481 caagaaagcc ggagagaaaa acagcagaag atggtacatg ttcttcccgg agggcgagta
    27541 caagaatcat ccggagaaat ccgaggataa tactcacctt cgttatgacg gtgctgtgaa
    27601 tttcgcttct ctgattgcta aagggttgaa agagatcggt ggaatctatg cagagatgct
    27661 gctggatgat ctgaaactgt aatcctgcag accattatga aaatacaaaa agtccgtttt
    27721 gcagacaaat tttgtcagtg aaacggactt ttttgtatct tgaaacaaga gtcgcccttt
    27781 tttataatgt ttctatcatt atgacagaca gggggagaag agtatgaacc cgtttcagtt
    27841 aaacagaagc acaaccttac tttcggatac cgtaacagaa aaagccgtag aacttgcctg
    27901 tgagcgactc cggagagaca tggaaaagac tttgactgac atagtcaaga atagaaacag
    27961 gatcattttg tgcaaaaaag atctgaaacc ggaacaatat gagctggaag tgacagaaca
    28021 ggagattacg atctacggag cggatgcccg ttcttttatc tatgcattaa attaccttag
    28081 tgaaacctac cttggagtgc tgccattctg gttctggaat gatcagaaga tggaagtgaa
    28141 atcttatgta gagatcccct gcgggatata ccattcggag ccggacagaa tccggtatcg
    28201 tggctggttt atcaatgacg aggtactgat cagccactgg acggcaggag tttccccaag
    28261 ctatccctgg gagatggtat ttgaggcgtt gctccgctgt ggtggcaatc tggtgatccc
    28321 tggtacggat aagaactcca gaatttacgc tcctatcgcg tcaaatatgg gacttatggt
    28381 tacacatcat catgcggaac cactgggagc ggaaatgttc ctacgggctt atccggatct
    28441 gaaaccctcc tatctgaaac acggagacct gttcgataag ctttggcagg aagctgttga
    28501 aaggcagaag gacgaggaag tcatctggaa tattggcttc cggggacagg gcgatgtacc
    28561 gttctgggaa aatgattccg cgtttgatac ttccgagaag cggggagaat tgatcagcaa
    28621 tattatgaag aaacaatatg caatggtcag agaacagatt ccggacgcag tgttctgcac
    28681 aaatctgtac ggtgaaattc tggaattata tcgggagggc tgtttgcaga ttccggagga
    28741 tgtgatcctg atctgggcag acaacggtta cggcaagatg gtatccagac gtcaaggcaa
    28801 tcataacccc agagtgtcag cgttgccgga ggaaggagat aagggacggc atggcaccta
    28861 ttatcatgta tccttctatg atttacaggc ggccaaccat attaccatgc tgcctaattc
    28921 catggagttc gtggaaaaag agcttacgga tgctctgagg catggaatta cggacctgtg
    28981 gcttgtcaat gcatccaata tcaagccgca tgtatatcct ctttctttta ttgctaatct
    29041 gtggaaacag gatgctctgt cggcagaaga gcatagaaag cgttatgtca ccgaatacta
    29101 tggggcggag aatgatactg cgcagctttc cattatggaa gactgcattc gcgattatcc
    29161 gagggctatg ttaccctttg gagagaaaga ggatgagcat gccggagaac aattctataa
    29221 ttatgttgtg agggatttta tctatagctg gatgaagaac ggagctgcag aaccggtgga
    29281 agaactgttc tggtgtattc ataaggatac ctttgctgca cagatggaat ggtttacagg
    29341 aaaatgtctg cagacggaaa aacagcttga gggtctgtat gagcgcggcc ttaccgttgg
    29401 ggaaaatgaa ttatggaaag attccgtatt gctgcaggtg aaaatccaca ggaattgtct
    29461 acagggagct atcttattta cagaagcatt tgcaacatat gaacggaaag aatataagaa
    29521 ggccttcttc ctgttgggta atgctgcaga agcatttgaa gcggcggaca gcgccatgag
    29581 ggaccgggaa cacggaaaat ggaaagactt ttatgccaat gattgcctga cggatgtgaa
    29641 ggagacagct tactgcctga aacgcctgat gggctatatg agaaatctgg gagacggacc
    29701 ggatttttac aagtggcaga gagaagtgac ctactctgag aatgactgca aagtggttct
    29761 gatcactaat atggaaaatc atatgacgga ctgggagttg tatcttgcag ggaaaagcag
    29821 acagtggtaa aaagcaaata gtaggaatat ttgtaacaat tacatgataa cagacacaat
    29881 tcatagaaaa aactgtattc agcaaaaaga ggaaaagccc aaaaacgtga taaaatgggc
    29941 tttttctcat cttaaaaaaa taaaataaaa attgtgtaaa aatgtgttga caatagcaga
    30001 cctttttgtt agaatagctt ttgtcgtcag gtgagcaaca cagatgacaa agacttaaga
    30061 cacacattcg gagagatatc gaagtggtca taacgaggcg gtcttgaaaa ccgtttgtcc
    30121 gaaagggcgc gtgggttcga atcccactct ctccgctagg cattcgtgcc gccgataatt
    30181 atatagatta ttagaatcaa ctatctggag aagtacccaa gaggttgaag ggacacccct
    30241 ggaaagggtg caggtcgtta atagcggcgc gagggttcaa atccctcctt ctccgtcagc
    30301 tgttgcaaga tgtcaaaaat acttgaaaaa agttgttgac aaacgaaagc ggttatgata
    30361 agctatcaaa gctgtcgctg aaaacaacag caaagcaaca cagctcttgc agagcaagag
    30421 gttatgatct ttgacaaata aacagtaatg caaccctgaa aattccaaga ttttcag
//
LOCUS       DAWDHD010000012        29350 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_129_length_29350_cov_13.266769, whole genome shotgun sequence.
ACCESSION   DAWDHD010000012 DAWDHD010000000
VERSION     DAWDHD010000012.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 29350)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 29350)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..29350
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129_length_29350_cov_13.266769"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         7327 a         7481 c         5525 g         9017 t
ORIGIN      
        1 aggatcttct tcacatagcc gtccactgcc tctgtggtga attcttcctc cttcggggca
       61 aaggttacgg tgaaggccat ggacttcttc tcgggtccca cctgtaatcc ctcatagaca
      121 tcgaagagtt ctacggactc cacgaagctg ctggcttccg cgatggcctt ctctacctcg
      181 ccacaggtaa cggacttgct cattaccagt gccaggtctc tggtctcctt ggcgaacctg
      241 gagatcggtg cgaaggtagg aaccttgccg tatacgggac gcagggcttt caggtcgatc
      301 tctgcgatgt atgcggactc acgcatgtct tcttccttct ggatctcgta ggtcagctgc
      361 cctaagtagc cgatctcttc gccgttgtac aggatctttg cggtacgata aggatgcagg
      421 aaggtcttct tgtcctccac atattcaaaa tcaataaaca gagtctctgc tacgatgtct
      481 accagacctt tcagggtgaa gaagctctct tccttgcccc agataccgat gcagatggtc
      541 tctctctcat cgggatactc ggtcagaggc agatccttag ggatgaattt attgccgatc
      601 tcatatactc tgccttccag acaaccgttc ttctggttac gggcaatggc atggatcatc
      661 tggggagcca gagtggtacg catcagagac agatcctcgt tgatcgggtt gatcagacgg
      721 atggcctttc tctccggtgc atcctcaggc aggcctaaca gattcagatc ggaaggagag
      781 aagaaggaat aatgtacacc ctcaaatgcg ccctgtgcac acagtgctct cttgatctta
      841 agctcggtct tctgtttggt attcatgccg ccgctggtta cagctgctgc cgcgaggaag
      901 gtcggtgtta catggtcgta accgtacata cggatcactt cctctgcaat atcgggatag
      961 gattccatat cttcacgata tgcgggtacc tgaatggtca gttcgtcacc gttaattacg
     1021 ggctggaagt tcagggattt taagattctc tcaatatcag cggtaggtac ttcaataccc
     1081 agtacaccat ttactttctt gatgcttgcc ttcatctcgg caggctcgat ggagttgccg
     1141 gtgctgactt ctacgtgagt agaggatact ttaccgcagc ccagctcctc gatcagatgc
     1201 agtgctctct tgctggccat aacggtagca tactcatcca cacccttggc atatctctgg
     1261 gaggaatcgg aagactgtcc cagtgctctg gagctcttgc ggatattgtc tcttgcaaat
     1321 tttgcggact cgaagatgac agcctctgtg gtgtcgcgga tctcggagtt caaaccaccc
     1381 atgataccgg ctaaagctac aggctttacg ccatcgcaga taacgaggtt attttcgttt
     1441 aattcaaact ctttttcatc cagggtcatg atcttttcct tgtcatgggc acgtcttaca
     1501 tgaatggcat tgccttcaat gtaagaagaa tcaaatgcgt gcataggctg acccagctct
     1561 tttaagatgt agttcgtgat atctacaaca ttggagatag aaccgatgcc caccagtgcc
     1621 agacgtctct tcatccatgc gggggattca ccgatcttta catcggatac gtagtgtgcg
     1681 atatatctgg ggcacagatc cggagcatcc acggttacgg taaatccgtc cttggtaatg
     1741 ccgctctcag tataatccag tgccggagtc ttcacaggct tattcaggat agccgctact
     1801 tctctggcaa taccgaagat gctctcacag tccggtctgt tggcggtaat ggcaatgtca
     1861 aagatccagt catccaactg taacaggggc tttacgtccg caccgacctc agcatcctcc
     1921 ggcagtacca gcaatccgtt gtagccagcg ccgggataca gatcctcgtt cagtcccagc
     1981 tcggtaccgg aacacagcat accgcaggac tcatagccac gaagcttgcc ttttttgatc
     2041 ttcatggtac cttcgatggt cacatgatct tttgcggtag catatacctg ggcaccttcc
     2101 agtgctacag ggtatttgcc gcctgcgtgt acgttatctg caccacaaca gatctgcagg
     2161 ggctctgcgc ctcctacgtt taccttacat acatgtaagt gggtctcagg gatcggctca
     2221 cactcttcta ccagacctac gaccacacca ctgatatcct tgcctgcttc ataggattcc
     2281 tctacctcaa atccgcagga aaataacttt tcctccagtt ccttggcttc tacatcaata
     2341 tctacatatt cttttaacca actaagtggt actaacatat cttttacctc cttcggcgtc
     2401 gtcttttacg acgtcctctc gtactattcc ttgcattgct actgttctaa aatcatattt
     2461 ccgtgatctc agtcatcatt gatctgatcc agtactctca tatcagattg atataacagt
     2521 ttgatattgt taataccgta ctttaacatt gccagacgct caataccgat accgaatgcc
     2581 agtccactgt actcctcgga atcaatgcca cagttctcca gtaccttctt attcacgata
     2641 ccggcaccca gtacttcgat ccagccggta cccttgcaca gacggcagcc acagccaccg
     2701 caggcgaagc agctgacatc cacctctacg gaaggctcgg tgaacgggaa ataagaagga
     2761 cgcagtcttg tggtggtacc ctcaccgaag attttctgta ccaatacttc cagcataccc
     2821 ttcaggtcgc acagagtgat ggtcttgtct actacgagac cttccatctg ggtaaacatc
     2881 ggagaatggg tcgcatcatc atcggaacgg aatactttac cggggcttaa gatcttaata
     2941 ggaggcttct gactctccat cacgtgaacc tgacctgcgg aggtctgggt tctaagcagg
     3001 aattccggag acaggtagaa ggtatcctgc atgtctcttg cggggtgatc ctgcggggta
     3061 ttcagtgcgg taaagttgta ataatcggtc tcgatctcgg ttccttcata tacgctgaag
     3121 cccatggatg cgaagatatc gatcagctgg ttacgcacct gggttacagg atggaggtta
     3181 ccggtcttgc ggctctttgc tggcatggag atatcgatct tctcactctc gtagcgcagc
     3241 tccatctctt tttcttttaa cttcgtgttc agctcgtcaa aatgctcgct tgcccagttc
     3301 ttcagttcgt tgaccttctt accgaagtca gccttctctt ccttgggcac ggaacccatc
     3361 tgcttcatca gtccgccgat aataccgttc ttgtcgagaa tggtcttttt gtactcgtag
     3421 acacccttgg tgttctcaat cttctccagc tgttcccgga tgtcggcttt gatctgttcg
     3481 actttttcat tcaattcgtt taaatctgcc atcttactcc tcatttctgt gtgctattct
     3541 gcacactgtg ctattctgca cactgtgcct tgttgcacac gtgtttttac ttgtgcctgg
     3601 gtgtgtattg cattttcata ttgtaactat tcagagcctc attcgcaaaa tcgcatctgc
     3661 gatgcttact tggagaacag gcttacctgt tctctgctca ttgaggctgc ttcgctattg
     3721 gctctgaata gttgcaaaaa aaccgcgcat acaaattcac cccattggga cgaattcata
     3781 tccgcggtac cacccaaatt ccttatgtga aaaatgaaaa ttatttttac attcacgata
     3841 agactcttaa ctcacgtaac gagtgaggaa acgtcatacc ctaccaaatc catgctctct
     3901 gctctcgaat tcttccggta tgcggctcgc gtgggaattt cgattctgct tgaacctcag
     3961 gaagcttttc agccggtggg cttccctctc tgtcaggaaa atagaatctt actgtgcacg
     4021 ttcattgcct ttaccttgat atgataatac cgaaatagcg gaaatgtcaa ggttttttca
     4081 tcatccggcc tgcttatact tctttccata aaattttttc cacggtgtcg atcactggat
     4141 atttctgtgt tccgatgggg tgtccgtcac gataggtgga aaatacatcg caggagatca
     4201 cattggtgct tctgtatatt ttctccaccg gtgtatgtcc aacaacctgt aaaaggctgt
     4261 cttcttcata cattgtctcc tgatgatact gcgggcggta ccagatgggg ctggcactgc
     4321 tccatagctg ctccggtccc atggtattta tctctgcgat gatttggtcc acagagtctg
     4381 tatttttcac attttcctct acaaatgcct gtaagatgcc gccatggcag aacaatacat
     4441 catcgatcct ctgtatgtac cgaatctgat tcccggccgg caatgcctcc ttaagttcct
     4501 ccagtttatt tctgaccgta taggctgccg cccgactgta tcccgattcc cgctcattcc
     4561 acagatagca tagatcatga ttcccgtaac accacagagt gtcggggaat tcctttgcaa
     4621 aagagatcgc cgcgtcaaaa gtctgcgcat acaattcaat gtcatattcc ctgttccagt
     4681 catccgggat gtccatcagg catactgccc gatccgccac accttttctc atccattcag
     4741 ccgcttcctt gaacatatac ggttttaaat gaacatccgg tatcacaagt gctttcatat
     4801 ctgcttctcc tttctgcccg ccaccaccgt agaaattcca tcggtgaagc ctgctccgac
     4861 acctcatatt ttcattttaa ttttactctc cggctctgat gatctccgct gccatctgcc
     4921 caagtccttc cgccccgtac ttatcccagt tcggatagac accgcggtag ttaatgcaat
     4981 agacaaggcc gtccatctcc agcatgaccc gactgataca tttcatatac tggctttctg
     5041 cgccgtagac aatttcatat tttccggccg caatgctggt ttggtcatac ggggtcaact
     5101 ggtagccgtc cggaacatat tttctcgcat agccttcata atacactcga ataccgtcca
     5161 ccgttttcag ctctgtcacc gaattttgtt cgtctgccat gacatccttc atcaactctt
     5221 caaaactttt gtgggcaatc ttctccccca cttctgctgt ttccactcca atggtgacga
     5281 tatcggttcc tttctgataa aaaagttcca acagctgatc tccgaaaagc ttctctccct
     5341 tctcatccaa tgcctccgta ttcaccaggg cagcactctg aaagtcaaat ccattggaaa
     5401 aggtttccac tgttctgaaa gtttttccca gatcctgtgc agcctgttgt aaatcctcat
     5461 aattcgcata ttcggttgtg atttccgtcg cacgataatg ttttacgatt tccatggccg
     5521 cacagccggt gaccagtaac gctgccagca cacacgctgt tgtcaggacc cgcctgcgca
     5581 gattgtaccg gttcccctgc tgtctgccgg taagtacctg ctcctgtgca tacagtcttt
     5641 gcagtaattt ttcctgcagt tctgccccgg gtctgatatt tacaaaactt tcttttaata
     5701 acatttccat tctttcatct gtcataacct gccgcctcca attcttcttt catcttttct
     5761 ctggccttat gtaacctgct ctttactgtt ccctgtggaa tccgaaggca ccctgcgata
     5821 tcctctatcg tcagttcttc cctgtagtac aggcacaagg gaatccgata tttctccggc
     5881 aatttccgca acgttgtctg tacatacctg atttcttctt tttttaacag atgatcttcc
     5941 acttcggatc gttccccgtt ccccccggct gctggctcca tgggttcctc actttccatc
     6001 ggtgcaatgc tgtttctacg gtttattttc cggttccgat ttttccaaat ccgaattgca
     6061 acagatagta aataactttt cggatttccg tctttttgta tcttgtccaa cagttccagg
     6121 gttttcagga aggtgtcctg atacaattct tccgcctccg gtctgtttcc tgtcaggtag
     6181 acgcaaaaac tgtagatatc agtgccatat gttgcaatga actgctctaa ttcctgtatt
     6241 gtcatataaa tttcctttcc ggtattttcg tctgcagtac gttaatgctt ttactatata
     6301 ttgtcctgag cttccaaccg gttcattttt atataaaaaa aatagaaagt gaagtgtgga
     6361 tttcactttc tactcattca gttttctgtc ttatccgttc tccttgatct ccagcatcaa
     6421 aagttccata gtcaggtcaa aatttacgtt agcatccaag cgcctctttg ccttatccag
     6481 ggcttcgatg acttcctcga ttccttcata gctgctgcgc tgggccacac ggcggatggc
     6541 actgatctct tcacggaaaa cgagatggtt cacatcactg gttgccttga acaggagcac
     6601 atcccggtac cagatggcga tcagatccag ataatcattg atctgcagct tatattccgt
     6661 aatctttttg atcgctgcgg tgatctcact cagatccata tcctgaatat atttcaatag
     6721 cgacaacgct tcattcttaa taatatcgaa gtcctcactg gacgccaggg atttcgcctt
     6781 gccgatatta cctctggcaa atgccacaca gacctccgct ttataatccg gcacctgcag
     6841 ctgatgcatg agataattac gcaccagttc atcggctacc ggcttcatgt tcagtaccac
     6901 gcaacggctg aggatcgtcg gcagcagaga attcacgtta gaagtcaaca gcatgatcac
     6961 tgcatactca ggcggctcct ccagcgtttt caggattgca ttctgcgcct ggggagtcat
     7021 cttctccgcc tcgttcataa tatagatctt atacgggctg ctgtaaggct tgatggcaat
     7081 atcgttgtta atctgcgcac gaatatcatc tacgctgatg gtattcggct tctcatggct
     7141 gacatagata atatccggct gattgtccga caacgcctgt ttacaggaat gacattcctg
     7201 acaaggctcg gttccgccct tttcacactg cagggtcatg gcaaatactc tggcaataaa
     7261 ttctttgccg gaggattttt caccgttgat gatataagca tgggaaatct tcttcgccga
     7321 aatggcgttc tgtaaatgct ctttaatctg ttcctgacct atgatgtcat gaaatgttgc
     7381 cattggtatt ccccctgtct gttatcttat gctatgtctg tacaccgtta atcatctgct
     7441 ctgcttctat cgtacttcct tctggccttt agcgcaaata aaattttgca ggatcaagta
     7501 aaaatatcca ataacagtcc ccggatctcc ccgatcttgt tcaggatcgc cagattgtct
     7561 ttttcatcct tcatcagttc ctgtgccagc tgatccagat tctcatccac cagccggata
     7621 atgccgtaca cacggtgccg tcctctccga tccaggaagt tctctctgga aaaagaatgg
     7681 gatctggtca ccacttcatt gagaaattcc ttgaccagtc cacgataatg ccgcatatcc
     7741 ttcacatccc gtttcttggc cagcttttcg ccctctgtgg tgatctccgc catcaggttc
     7801 gtcagtctct cctgcagttc tgcttcttcc acatggctcg ccagcatgaa cttgaagctg
     7861 ccatccgtgg gcgctgtgtt ctgtacctgc tccacggaag ccggctgctg tatctgattt
     7921 actctcattt ccatacgccc acctcttttc ctctgtttac cggctatatt ccggcatttt
     7981 tcttctctgt tatcagtatt tcggctatat tccggcttct tttaagtact ttgtaatatc
     8041 tttcagacat tttgtcagat ctgtattctc gaatctgtcc cggatccctg ctgccgccag
     8101 cttttcttcg gagaaatctg cgctgtccgc cagaaatctg cgacacatct cttcgtactt
     8161 cggcttctcc tgtcgcagtt ccctgtccag tgcccgctgc aggcgtacac cgtcatccag
     8221 ctcaatatag accggcaaaa tagcttctgt tccgaaataa gcaatcattt tctcataggc
     8281 ttccagtgtt ccgatcatga gataattctt cgtggtcaaa tctatctgtc cgtcatccac
     8341 tgtaaaatac cgccagatgc catagaccgt attataagct ctgtcctcta tgacctttcc
     8401 ctgttccctc agggtctgaa aaccggtctc atctgtgaaa aaatattcca caccgttctc
     8461 ttccccggcc cggatcggtc ttgtcgtata aggcacgatg gtccgaagat ccatcgtgcc
     8521 ctctttcatc agttctttaa atatggtgtc ttttccggag gaacttttcc ccatgagata
     8581 tacaattttt cccatatctg cgttctcgtt ttcccgctcc tgtttcggct gtttgaccgc
     8641 cgtacagtac gtttttattc tacttccacc acgtggatca tatcggtctt acctaccacg
     8701 cccagaggta cgcctgcggt gatgacaacc gtatccccgg ttttcaccag acctgcctgc
     8761 tttgccttga ataccgcttc ctcgaacaga gcatccgtgg tccgctcctt ctggatcatg
     8821 acaggtttta caccccacag cagatttaac tggcggtata ccttctcatc caatgtacag
     8881 ccgatgatct ggcatccggg cttataacgt gcgatcatat tcgcggtaaa tccggacatg
     8941 gttacggtga tgatggctgc tgcattcaga tcggaagcca cactgcaggt tgcataggag
     9001 attgccgtgg taatatccgg agtttcctgt ctgtcttcct tgactctctg cattcttccg
     9061 cgataatcga tatcctgctc tgctctctcc gcaatgcggg acatggtctt aacagcctct
     9121 accggataca gaccggctgc gctctcgccg gaaagcatga tggctgtggt tccgtcatag
     9181 atcgcattgg caatatctgt tgcctccgct ctggtcggac gtggattctt gatcatggac
     9241 tccagcatct gggtcgcagt gattacatgc ttgccctgtg ccactgccat cttgatcatc
     9301 ttcttctgca gaataggtac ttcttccaga ggaatttcga cacccatgtc accacgtgct
     9361 accatgatac cgtctgctgc agtaatgatc tcttccagat tctggattcc ctgcatgttc
     9421 tcgatcttgg cgatgatctt gatcttgctg cctctgtcct ctacgatctt gcgcacttcc
     9481 tgaatatctt ccctggttct gacaaaggaa gctgcgataa agtcaaatcc gtgctctaca
     9541 ccaaacacaa tatctgccag atccacctca ctgatataag gcatggacag aatcgctccc
     9601 ggtacattga cacccttatg attcgataca aaaccgccgt ttatgacctt acatacaatg
     9661 tcttctcctt tgatctcttc taccgtcatt tcgatcagac cgtcatcgat caggatcgtt
     9721 ttcccgacat tgatgtcatt tttcagattt ttgtaggaaa tagtggctct ttccgctgtt
     9781 cccatgattt cttccgttgt caggacaaac tgctgaccgg ataccagctc taccttaccg
     9841 ccctcaaaat cacgcagacg aatctccggt cctttggtat ccaacagagt tgctaccggc
     9901 aatcccagtt cttttcgtac tttcagtact ctgttgtatt taaccagctg ttccttatgg
     9961 cttccatggg aaaagttaaa acgtgccacg ttcatgcctg caagcatcaa ttctcttaat
    10021 ttctcctcac tctcactggc cggaccgatg gtgcaaataa ttttggtttt tctcatacag
    10081 gctaaatcct ttctgctgct attttttttg atttttgact acggtaatgc aatattcgtc
    10141 tttttcggtg actccctgta ccttcagacc ctttatgaaa tactcctcaa gctatctgca
    10201 atgaatctct tcgatctttt ctcccggtac cagcaccgga attccgggag gatacagatt
    10261 cacaaaatct cctgcaatcc tgcctgcagc ctgtgccaga ggaatctcct ctttttcggc
    10321 atcccatgca tccgcaagag acagaatgac ttccggctgc atgtgataga gagatgtctc
    10381 tctggaatct ccgccttccg tctgtggctg cgtctgccac tttcccgcag atatgtcacg
    10441 atcgatggca agcaatgcat ccgtcattct tgtgtatgcc tcgtcactgt ctcctatggt
    10501 gaacatcgca agacaatgat ctgcagctgc catttccaac tgcaagtggt actcctctaa
    10561 tagaatatca taaatctgct gtcctgacaa acctgccttt gaagataata tcaccagttt
    10621 tccgatatcc tgtctggcat ccagcggcaa gaaatgaagg ttcctgcatt ctgacagttc
    10681 tgtcagcatt ctcagataat tcacctgcaa ccgggcaaac agataatctc cctgctcctt
    10741 taccagctgt agtgcattgt caattcctgc cattagtaca taggatggac tactggactg
    10801 ataaatatgc aaaaacctgc gcaaacgttc tctgtcgacc agtcttccgt tgacatgaag
    10861 caacgcgctc tgtgtcagtg ccggcaaagt cttatgaaca ctgtggatca ccagatccgc
    10921 gccacactgg cagctgttct tcgcaaatcc ttccgccagt cccagatgag caccatgggc
    10981 ttcatccacg atcagaggaa ttcctttact atgtaccagc tttgcgatcg tctctatatc
    11041 tgcgatcctg ccttcataag tgggtgacac cagaaatacc gcatccggaa caggctcccg
    11101 gctgaatgcc tctgcgatct gttccggagt taccgcatca taaatatcat actcctccat
    11161 catttccgga tacagataag aaactgttaa atttctcaga tatgccgcat gatacgcaga
    11221 tttatgggct cctctggtca taaggatccg cccgcccctc ggaaccgccg cactgattgc
    11281 cgccagaacc ccgcaggtac ttccgttgat aaggtaaaag ctctcgtctg cgccgtacag
    11341 tccggctgcc tcgttctgta atcgtcttag gatttcctct ggagcatgta gattatcaaa
    11401 tccatcaatc tccgtgatgt cgatatccat gatctcccgg gggaattctc cacaaagcag
    11461 tctcttatgt cccggcatat gataaggata ataatctgtc ttactgtagt cttttaatct
    11521 ccggtataat tcacccaatt ccgtcattct cctgttttgg gtttccaccc ttttattttc
    11581 cctgctcctc ttcaatcttc cgaagcttct cgatcagggc tccatgtccc ttatgtacat
    11641 gaaatgtctc cataagcgaa caccatttgt ctgccatggt gacgatccag gcttcacgac
    11701 acatgggaac tgcggagatt gttagcggcc acatatgttt ttttataata tctttttccc
    11761 tgggagtgag atggtattcc ttctgtgcat tgtgaagcgc tcttcccgga tgataaaagc
    11821 catgaagctt atgggggctg atatgatccg gcgtgtgcca gtcgtacaga aagtaatcat
    11881 gcaataatgc cccacggatc aaatcctttt tttcacaggg aatatgcagc ttctcgctga
    11941 tggcaagact atattttgcc acattcatac tgtgatcatt gactgtcatg ttaccgtgct
    12001 gtacatgtga acgtgtggag cgaaagttct tagaacgcag aatatccggt gctacagccc
    12061 ggatctcctg ccttaattgt cgttctcttt caaatctctc tttccacttc tgcacttttt
    12121 ttgctgaacg ctgtttcata gaactgcctt ccttctcaat atcccatcat ctatagtcac
    12181 actgattcgc attaatagta atagcatact accgggaaac cggtagatac atattcataa
    12241 atagctgccg ccatattcag tggcagattg acacaaccgt gagagcctga tgtcagatag
    12301 atggttccac cgaagcttcc tctccaggta gcatcatgca ttccgacatt tccgttaaaa
    12361 ggcatccagt agtttaccgg cgtctcataa tccgcaccgc gtaatacggc attttttgtc
    12421 ttataggtca gtccgaacac tccgggaggg gtcatgcagc ccgctttgct catattaccg
    12481 gatacaaaat ctgtctccaa tacaatgctt ccattctgga acagataaag atgctgattc
    12541 gtcagatcaa tttccacgta ggaattaccg atatcgtcac ttcccttttg tgctcccttg
    12601 ctgctgtata ccggttcctt gtcgatgttt tctccctttt caatagaagc tgtcagttct
    12661 ttgacctctt cctcgcgatc ggtcttccag ccgtaagctc cgctgggcaa ggtaagctcg
    12721 attccctgaa ctgtggtaaa ctttctcttt ttgccatagg tatcgtattc tttggcctgc
    12781 tctgctacga attcctcgat tgcttcctcg tccagttggg gatccttgtt atccgtctgg
    12841 atccactcat ggatcgtatc tccgtctacg acaaccttat tgccgttcca gtcataggtg
    12901 atctgtgcgc tgacccattt attcatggta tcacaggctt ttaccagcgc tgcatcctct
    12961 gccgttattt tggcagtttc ataacagccc tgctcctcca gatctactgt ggtatctccc
    13021 tgcatgattg ctgtggatac tacttcttct accaggttgt tgttcaactc tattcccatg
    13081 gtctccggaa tgatttcata attcttgttc ttctcggaat attcactgat ataagcatct
    13141 tccggtgcaa tcatattttt gttctgcagt gcaggcatct gtgccagttg ttcctgcaat
    13201 ttgtctgcat cataggacac accctgaact acatcatgat tctgcgtact ccacagcatc
    13261 tcaatccaca ggaattcatt ctgtctgttc aaaagctcct gtgccgcatt caaagtattc
    13321 acccagtaca ttccgatctc agatgctgtg atcgtaccga tctcttcctg cacaccgttc
    13381 tcatctctgc ccagaatctg taagctatac tgctgtgact gctgatccat gatcgccgct
    13441 acctccatcg ccgtcatatt actgcaatca ctgttattga tataagtatg attcaggaaa
    13501 tgagactggt agtaaattgc cacaccacca tatgccaata gaattactgc aactacagct
    13561 gctacaatac caatccattt tttgctcttt ttcttctgta ccgctgtctc ttccacaggc
    13621 tgttttgttt cctctttgct ctccgtttct ttcttatcag cctctttctt tgttgtttct
    13681 tcttttaatt ctttgttatc ggtttccatt ttatcttctg tttctccctc atcttatttc
    13741 tgttgcgctc attatagcat ttaatttcgt aaaaaaacag tgtgaaaagg ctgaaaaaaa
    13801 gccttttcac actgttttag aatactactt gcactaatat catataaagt atggtcccta
    13861 cagctatact cagcaacgta tttttcttcc agatatgaag caatgccacc acggcaatag
    13921 acagcaattc cggaaatcca tgactgccgg taaacagatt aattcctttt acacagtaaa
    13981 taacaagaat caccataatg gccggcggga gtacttttcc cagatatgtg actgtttccg
    14041 gaacctctct ctttccacca aatgccacga aaggaactgc ccgtatgatc aaagtaacaa
    14101 tagctgcaac tgctatgatt gcgacggaat gtaacatttt ttcttaactc ctaataatat
    14161 gattgcggca atggtcaatg caggtaataa aaaggtgtct gcgcccagta ccagtaaaca
    14221 cagaactccg acaacgcctc cgatgactgc cggtttatgt tctttcgtat ccatccactg
    14281 gttgactact ataactgtga ataatgccgt catggaaaag tcaattcccg tagtgtcgat
    14341 tggcaggacc cttccggcca gttcacctac acagcttccc agaatccaat agcaatgatc
    14401 caacagagaa atgtagaagc tgactcgttc ctcacgcatt ccctcaggaa ctttcagatc
    14461 acataacaga gaaaacgtct catccgtaag ggaaaagatc atataaggat atgttatacc
    14521 cgtttttttg aaattttcta caaaagaaag tccgtaaaat atgtgtctgc cattgacaaa
    14581 caacgtcatg atcagtgtat accacagact tgctcccgcc gtcagcaagg tgaccaacac
    14641 aaactgcatg cttcccgcat acacaaacaa gctgataaaa aaggcccacc agaagccgta
    14701 tcctgcatca ttcagcatca gtccaaaggc aaatcccagg aaaagatatc ccagcatgac
    14761 tggtatggac tgtacgattg cgtaacgcaa ttctttcatt ttttattctc tcttacaata
    14821 taaatcgagc cgcttgtttc tgccaagccg ctcgatttaa gattttatca tattcgattg
    14881 ttacacacaa tagtcgaatt tatcctttta atccactggt tgcaatacct tccacaaaat
    14941 acttctgcgc tgcaaagaat aagatcatga ccggcactac agcaaccgtt gccattgcca
    15001 gtactttgcc ccattgcacc acagattccg catccagcga cattctcaaa gccagtgaaa
    15061 tgggatactt tttcactgaa ttgataaata tcagggaatt gaaataatca ttataagtcc
    15121 acaaaaactg aaatagtcct gcagaaaaca atgccggttt catcaaaggt aaaatgatac
    15181 gtgtcagcgt cttaagtgtt ccgcagccgt caatgtatgc ggattcgtcc aattctctcg
    15241 gaattccccg taaaaactgt atgatcatat acgggaaaaa ggaattacaa cacaataacg
    15301 ccggtgcata aaagggcata taggaatcta cccattgaaa cttgttatac agcgtatatc
    15361 tcggaatgat taccactgca ttgggtaaca tcatcattgc caacaataat acaaacagaa
    15421 ttttctttcc ggggaaatta aatcttgcaa agccataggc cacaaaagtc gccgacaata
    15481 ctgtcagtaa tgtcgtggga attaccatca gtgcagtatt ggcaaagaac tttgcatagg
    15541 taaattttcc gcttcccttc catccttcta tataatattg aaaggaaaat gagttcggca
    15601 gcaatttgaa ggaaccgaag atctcctcat ttgttttaaa tgtcgaaaag aacatccata
    15661 taatcggata taaaaggata aatccaatta caaaaattaa caaataatct agaaacagat
    15721 ttcttttttt ggatttcata gtacggcctc ctcatatcct attcatctgc ataatgcacc
    15781 caaagcttgg aggatgcgaa caaaactaat gtaaacgtta aaataacgat aaatacaatc
    15841 caggaaatag cacttgcata gcccatctta aagtatccaa atccttctct gtacagcttc
    15901 attcccaata cataggtaga tttcagaggg ccgccttcgg tgatgacatc tgcggacgta
    15961 aaattctgta atgcctgaat cgtctgattc agtaccgtaa agaaaatgat cggtgagatc
    16021 tgcggtaatg taatgtgcca gaatctgtct attttcttag cgccatcgat ttccgctgcc
    16081 tcgtacagag atttcggaac attctttaat gctgccagga acattaccat ggacgaacca
    16141 aactgccaga tctccagtaa gcatatggta ggcagcgccc atgtagtgct tcccaggaat
    16201 tgcactgtcg gcaatcccat ggcatttaac accgcattaa tgggaccgtt atctaaaaac
    16261 atcagtctcc acaaaatagc aattgccaca ctgcctccga acagagacgg aatataatac
    16321 agcgttctga tcagattaat gcccttaatg tcacgattca gaaatattgc aactgctaat
    16381 gccagaataa tttttcctgg tactgtcatt aacgcaaaca ctatggttac tcgaagagaa
    16441 gtccaaaatt ccttatcctt cgtaaataat tttacataat tcgccaaacc tacaaatacc
    16501 ggatcagatc ctatggaata gtctgtaaaa gaataaaaca gggaactgat aaacgggtat
    16561 aactgaagaa tggcaaaacc aagaatccac ggagcaatat aataataagc ttttagatca
    16621 cgcttggaat agctggattt tttactgaga gtttttgtct tgctcatatc catactcctt
    16681 ctaatttaaa gggagatgtc ttgcacgctt tcgatcagac atctcccggg atcgtaattt
    16741 ttacagactg gaaagagaat ctataatctc actcgctgct gcatccggag ttgttgcacc
    16801 atatcctatg gtctcaacag catcaaacaa aattgttttc gattcttcag aagatgaaat
    16861 cttatcattc ggagttccgc ctgctgcagc tgcaatattt gcgccctcca tggttacttc
    16921 actgagctta ccgttctcgg aacagatctt tctcgcattc tcggtaggag gtacagatct
    16981 ggtagcacct aacgttgcca atgcagtttc attgttaaag aagtaattca taaattctac
    17041 tgccatctca ggatgttcac aggtcttggt aattgcaata acctgcggag tattggatgc
    17101 ccagcctgcc tctgtacagc catccagcat cggcagctgg ctcatggtgt agtttgcact
    17161 gtcattggct gctaccatga catcaatggt tgaaatatag gttaatgcac atacatactt
    17221 tcctgcgatc cagttggcat cagactgtaa gttgtctccg ctgtaagcag cctggtaaga
    17281 tgcaggtgct acgacttcct catcgtacag cttctgtaca tactccatta cctgtttcat
    17341 ctgctcttcg gtaatgttta actgaccgga ctctatgtca aacagcgttt tgccacataa
    17401 ctgcttgcca tagttgaata caaccaggtt tacaatgtat tctttgtttg cacagagcag
    17461 atacatactg tcatcatatt cccgtacctt tttgcccatc tcaaacagat catcccaggt
    17521 atatgccttt gtgaaatcca ggccaatggc atctgccaga tcctgattta ccaaaatgcc
    17581 ggcacctgaa attccggtag gaagtcccaa ctgcttgcca tcatatctgc cgttaatctc
    17641 caaagagata taattttcat caaatgtgga taaatccata ttgctgtaat cctggtaatc
    17701 aataaaataa tcaccggtat ttacatactg agggaatatc tcaggatcca cctgtacaat
    17761 atctgctgcg gtaccggaag ccaactgtgt cgctaattta tcatggtatc cgtcgtttcc
    17821 gccgtattct gcttctatgg ttacgttggg atgcaactgc tcgaactgtt cgatgacatc
    17881 taatgtcgct gcatttctct catctccgcc ccaccaggag aatctcaaag tgatgggttc
    17941 gtcaccggag tccgatgtct gtcccgctgt agtctgctgg gtagccggtt gcgtagatgc
    18001 ctgtgcatcg tcggaagcat tgccgcatcc agtcaacatt cctgcaatca ttgatacgct
    18061 aagtcccatt gccaataatt ttttgttcat aaaaacctcc ctttctaaaa tacctttaag
    18121 ataaataaat tatatctctt gaaagaattt ccagaaaggg aggccgcttt ttatttttcc
    18181 ttatttttta ttgatttttt tgcatcaagt catttttttt tacattcatc catttttttt
    18241 aagatagcgg caaataaaac ttaactaccg tacccatgtc ctttttactg taaatatgca
    18301 gttgtgcttc ttccccatag tatagtttta acctgcagtt gacattagca agacccacat
    18361 gattgggatt gtaacggctg atggttttgc gctgtgccat aagttcttcc tttgtcatgc
    18421 cgattccgga atcaatgacc gctacaataa tcttctgtat ttgctttgat tgatatattt
    18481 taattttgat atatccatgt tcccggtatc gcacaccatg taatatacta ttttccacaa
    18541 tgggctgaag taataaacga aatacatgat gctccttaag attttcctct atttcgtaat
    18601 acagaatgaa cttgtcccca aaccgcattt tctgaatatt gacatatctc ttcaggtact
    18661 gtatttcttc cgacaacgat acctgctgtt tggggtctcc cagcgcatac ttaagtatgt
    18721 cgctcagttc ctccgtaatc tctgcaattt tgctgtcatt tccttccttt tgacgaattt
    18781 caaactgaat actttgcaac gtattaaaca gaaaatgggg attgatctgt gcctgtaacg
    18841 ctgtaatttc tgcgatacgt tcttcctctt ttttggtttc cagttctttg tccagtctca
    18901 ctttctgcaa aaacagataa atgatattct tcatcagaat gtcatattca tctcttgccg
    18961 gagaagtcgt ctctatggga agatatattt ctttttccgc attggagaag atctgaatca
    19021 tatcatctaa gtgggaaaaa tttctcttgg tcgttatgta ggccaatgca atcactatca
    19081 tggtattcag tgcgaataca ataaaataga tgggatagat cgaacttaat tccttaaaaa
    19141 tcgctacctg gggaagtaat gcgatcaatt ccagattaca tttttcatca taaatctttt
    19201 ggatcaaata tctttgatga tttatggtga tccaatttga cgtatcctct ttttgtgtaa
    19261 tacggctcag gttttcactg tcaatattca cagtatcctg atacgcaatg acaagatcct
    19321 gctgactgtt cacaaacaaa atcgtcattt ccgtatcgcc caccgcctgt ctgagaatat
    19381 tttcataggc ctgcagatca atactcataa taacaaaccc atccatcaaa agcattctct
    19441 gataaacggt cagttctctt tttgtattcc tgtattcttc atattcgtgg tgcataatca
    19501 gcgtctctgt ctccggctcc atctcctgac actctttcag ccagtactca tctccgttga
    19561 ttgtctgaat gccgttctca gaagaataat atcggtcata tccattgaga taaatattga
    19621 tattggaaac atagtcatac acttcttgta cacttcgaag catggctttc gtactccgca
    19681 ggtaaatagt atccccatag gaaatataat ccgatttttt taatatattt tttaaagcaa
    19741 atcccatgta tggattcttc gtaaacagat cattttgcat ggcaatatta tttacaacca
    19801 gagacagatt ggtatctgta gattccagca ctttctgttc ctgactgatc aaacgttgtt
    19861 ctgcattcct tccgaatact accatggcaa ttacaaaaag aagaaatgtg ggaataaaaa
    19921 atgcgaaaaa ataaactgcg aaccgcttca aaaagaattt tcttttcatt ttccggtctt
    19981 tccttttcta tagtccatcg gagtcctccc atagtaccct ttaaaagcat gtaaaaactt
    20041 ttgggattgt catagcctac atagtacgct atatcgtagc tcttatagtg tgagtccatc
    20101 aacatttccg ctgctttctc catccttatt ttcaccagat actccgcaaa tccaaaatca
    20161 catttctctt ttaaaatgcg ggataaataa ctggcactga gatttaccct gattgctgca
    20221 gcttccagag atgcctcctg aaagttatcc tcgatatatt ttttgacttc atccagtatt
    20281 ttctcgtaat aacttttatt ttgttccggc tctatgtgat ttctttcatc caattcctgt
    20341 tttattctct caaaacacgc aataatatct gcacttttga tgggctttaa taaaaaatcc
    20401 gccacattta gttgaattgc gcttcgaaaa tattcataat tctggtgact ggatataata
    20461 acaactttcg ttcccttttt ggatacctgt ctcgctaatt ccagtccatt tatttgtggc
    20521 atctcaatat cactcattac cacatccaca tgattatttt gaatatattt cattgcctct
    20581 gtggaatcac aaaatgcctt cgccgcaaat ccaatctccc gccagggaaa taaatttgct
    20641 actccttctg ctattgttac ctcatcatct acaattatca gttgatacat atacagcaac
    20701 ccctatacgt tttttcaatc actatatttt atatagcatc ttaaacctta ttttacatgg
    20761 ctgtcttttg gaaagcaacg actttattgc tcaaaaaaag accaatgttg cataacattg
    20821 atctttcaca aatgagacac gggggattcg aacccccgac aacttgatta aaagtcttag
    20881 acagatttgt tttcccattc gtcatggttt ttgtatcgtg taaaatcagt caattatggc
    20941 tgaaagtgtt ttctatcttt tgtgctatgg catattattt tctaaattgt tagtcattta
    21001 ttagtcattc atactcttca tggctacccg ttccgtgctg ctgatccatg tgtattcgaa
    21061 cgtgtcaaga aacagaacag ctgacactaa acataatttt acacttagtg ccagccgcag
    21121 aggttaccat tccgaaaccg cttttcccaa tggaacacta ttattataca tctattctca
    21181 aagacatctg taccaaatga aaaaccgccc tcatatcatt atgaaagcgg ttttctgctt
    21241 gccctctacg gagcaaatag caactcattc acctttaaga agataaacat aaaagaagaa
    21301 atatatatca atcagtgcgt gccctgatca ataccactgt aatatgaaat cctgctatgc
    21361 agtgatctcc tggtaataga tgcccttttt cttattctcg aatacatagg catcgtatac
    21421 aatacgccct tctaccaaag taccgttaat tccgggcggg ttcttatgct cgttatagtc
    21481 ttcaagctgt gtaggtgcac aacacgcaga cttatgggag agcataaatc cgaagttctc
    21541 cggcagcctg gatgctgtaa ccttaatagt cattgctccg tcaaggttgg caataacacc
    21601 tctgatccgc atctcttctc cgatctcagt ctccattacg atatccttgc actttttcat
    21661 aagcagatag gttgcagggg ttaccgtaag tgctctgtcc gtctccggta ctagatcatc
    21721 atccaatgca gcagatgcct taatgatctc atcgtagatg ttatccttgg tcaatgctac
    21781 cgctgcaggt ttatgccctg cccctgctgc catcacacca tagacatagc cgtctacttc
    21841 cgggataaca acctctctga cctgtcttgc cagcgctgtt gcaccattca gtgcaccgcc
    21901 tgtctggttc tcgtccagcg catcaatcac gaatgtgaat gatctgtctt ttttaagggt
    21961 agctgtctcg caagttccct ccaggttggc aacggcacca tatcggctcc agttggatcc
    22021 tgttgcacca gtcctgccat agtcattcat ttccgctgta ttgaccttat agagctttac
    22081 tgtctttgct ccatcaaaat caaaatcctc attcgttaaa atgcttttct tactttcctg
    22141 agagaaaagt tcatccacat agggtaaata ctttgtagct aattctgtag acacgttatt
    22201 ttcctcttaa ccccatagcc tttcggatac tggaagaatc atcgttttcc tgcatatgaa
    22261 tggatgtacc aataatacgg gatctctttg catccagttc tttgctccag ccttccgggg
    22321 tcctctcttt gataatatta tcaatgatct ccattgattt tttcagttct tccgaattgc
    22381 cgcatctgat aacatccgcc agttcatcag gataacccgc gcttcgcaat atctccaatg
    22441 tatttaaccg gttttctttt gcttcaagat ctgcggctcg cttgtccagc tctgctgttc
    22501 tctgatcaag ttcctcgttg ccagatgcct tattcttttc ctttgccaga cgttcctgca
    22561 caatccggtt cacatcttcc tgcgtaaata tctttccgct cttctgtgta ccctctgtgc
    22621 ttgtctgtgt gccctctgct gaggtgtttt gtaaattatc catgaattta ccatcctttc
    22681 attttacgcc tgaaataagg ctattatttc cgctttgccc tgcgtaaggg tggctgttat
    22741 acactcgcca tagtggcagc caggcgctgc tctatattgc ttatgtaccg gcgccgtttt
    22801 cggtaaaacg aatttctact tgccggaatc acatttcctt tacaggcttc ctgcatttca
    22861 attacatcaa aagacttatt ttcgctaatg gaacgaaaga gcatttcacc tataccatcg
    22921 tcaatactgc ttgccatatt tcggagttct gtatccggta tactttcaca tttctttcgt
    22981 aacgattcta tgtactggtt cacgccgtca tctccttccc acatatttat tttaatataa
    23041 gtctgttgaa aatttgtacc attgtaccaa caaaagtata tagtcaataa tttcaacaag
    23101 attcaacagc ctataatata taatccatat agtttctgta ggctctctat atgcaaaaaa
    23161 gccattatac gcaactatat gtcttaaaaa gtaatctttg gtaaacaaaa aaggcagcca
    23221 gcacacacct gcaccgctta ccttttctct gctctctatc gtccggatcc gctgttggcg
    23281 cagcatcccc cggaaggata ttattccatc ttcactctct cccgctgcca tgccaccgcc
    23341 tgtgccacgc tctccggctc cctgccgttc tcccgggtgt agttcctcac tgcattcttt
    23401 ctggccagct cctgccaccg ttcatcggac atctgcggga tctccagtac taatggagta
    23461 gttctgatct ccgtaatctc atatttaata tttttcatct tctcacactt tcactataat
    23521 ttctgcccgt tttccgtaaa atggaatatc ttctgctgta tactctacaa ggaccgggga
    23581 gaggaagtgc agcacggttg taagctgctc ctcttcctca tagtccaggg tgagcattac
    23641 ccggttactc atataatgcc tttatctgct ttatcagttc tccctgcctg cagtagatat
    23701 gccacagggc tgctgccgcc tcttcctcat cagtagcaga aggaccttct gcaaaagcaa
    23761 cctgcataaa tccgatcagg gcattgagct gatacagttc ataaacaaca ccatctaact
    23821 tattgagatc ttttccgctc tcaatcttct tcatctattt gccctccttc atctgcttca
    23881 ttgctgttat gtaaccaaag taaaaagcat ctttctgtac cgcttctgca taaacgttga
    23941 tagcatcatc cagcgcagaa taagcattct gtgtttcctc gcttgccatc ggctggtttt
    24001 cctgttcctg ctccatgatc tccataattg ccttgtaatc cataattttc tttccttcct
    24061 gccggtactc tgctataata cagaggaatc cgggctttat gcgtgttgac gggttccatt
    24121 gccctgtgac ggtgttgccg cactgtccgg ggcgtttttc ttttcttcaa gcaacagttt
    24181 cacaaactcg ctacggttct tctcctcttc cttttctgct attgcaatgg tacacatagt
    24241 taaaaagtgc ttgtcctccg ggaacatttt tccatactgt ttctgaatgt ttcggacctg
    24301 atctgcgctc atgctactca gttctctggc aatctcttca tcggtcattt tttccattct
    24361 tttttccctt cctttcaatc aaaataatag tttctagtac caggctgcga ctatccgtcc
    24421 catactgctt tacaatttct tccgccctgg cctgcagttt ctcccactct tcatccgtct
    24481 gcgcctcgtg gtactcccgg aatagcttcc agagatcggt atagagggcg tacacttctt
    24541 tcagttcttt atctgtcatt tctcgccttt cccgtctaaa tatggaaata tatattcata
    24601 atattcaaaa cattcatttc acgaatatta tgaatattaa gaatattatt tttgtcttta
    24661 gacagacacc tttttaaact catgaatacc tgaagcattg ccatgtcctt tgtcaatatg
    24721 taatattcca tcatattcat ttagtttatt gctatatttt tttaatgttc ttccgcattt
    24781 ctgaggactc tggtaaatcc tacacccctt cagatactcg cttgccttta tgatctgcgt
    24841 aatcgtacct gaataagatc caccgttcgt ctctaccagt ttcttaattg cctttataat
    24901 aggggtctta tcatactcat ccttggctct tttctcggca atttcctgcg catctcccag
    24961 catttcccac ttacacaatg gctcgttaaa tctgatcgca tattctccgc ttcggaccgt
    25021 tctgccggtt acagaaagcg ttgcatctac gtccttgcgt tccttcttct gaattacaag
    25081 catctgatca ctcgctccca tgattgctga acttcccaaa gcatttgaga acggatctgt
    25141 cggatcttca ttcttcctat tgtgcataac aagcataaga gaaatattgt acttatctgc
    25201 cagttccttt aacggtgtga aattctcgta aatgtcatca taatcagatt cgtttactct
    25261 cttaggcttc ttgatctttt ggaacacatc tactatgaca agtgacacct tcctatgcat
    25321 ctccaaatat tctgtaatat ctactagaaa gctgtctctc attgtagaat aatcgttcac
    25381 aatcacaagg ttatcaggag gctccatgcc atttaaaagc gtagtcaatc tggattgcgt
    25441 aagagcttct gtattctcca gatctatgta taacacatct ccctgcgtcg tgttgaatcc
    25501 taaaaaatca ctaccatttg ctactgctac cgccagctgc aatgccaacc agctttttcc
    25561 tattttagat tttgctgtca atacaacaag accttttgcg ataatgacat ccacaatata
    25621 ctccatcggc ggaagatctg cggtaacaag ctcccgcgcg gttctgtata caagattctt
    25681 tcgcttcttc gatggtctta cctttccgcc atctggaacc gctgctccat gagcatctgc
    25741 tttaactctt cttcgctcaa gctctgcaag tatttctttt tcttccattc gtactccttt
    25801 accttctttt cataatttgc attgatctgc cgtgacaggt cctgcgatat cgtccgcagg
    25861 atctctttct gtcggatttc ctccagttct acgaatatgt catacacatg gcgcacatga
    25921 cctcttccac ggctctggaa gccgctcaga acgtccgcaa gaaactcttt gtcatataga
    25981 ccatacccgt atagtttcat gaagaaatcg cggcacagag gggcctgcca gtcaatattg
    26041 ttgtagaact cctgctttgt catttgctat caccggtctc cagatactct gccaacttgt
    26101 ccagatttac aaggtacttg gttcctaccc ggacatgaac caccttccca gtgatgcaca
    26161 tcttacggat tgcgctgtac gggatcccgg ccagtgctgc tgcctctttc aaggttccca
    26221 tccggggaat tgctttttct gttgccattc tgcatatctc ctttcctgca ttataaaacc
    26281 atgagcaatc agccttattc ttttacatat tcttcagata tcattaacgg agcttcaccg
    26341 tcagcgcagg ccggattgta taccacatca atgccaagcg ttactgtgtc cactctacgt
    26401 tccagctcca atatcatacc ggtatcatat tcattgatac ctgtagttac ggatgccacc
    26461 gtgtatcctt ccatgatctt ctcattgatt tctacataat ctctaaatac ttttgctttc
    26521 attgttcttt tctccttttc caaactattc catttactgt tcaattattt cgaccacatc
    26581 gacatcaaga gctttcgcaa ctctaccggc acaaacagtg gtcactacat tactattaag
    26641 aataatattc atcctcgatc tgcttacccc ataaacattt gcaagatccg ttactgacat
    26701 ctgttttctc gccagttcaa tgtctatttt gcttctgcat aacttcatta tttcacctcg
    26761 ctttctacat aacactgttt cagtgtttta cattgacact ttaacactaa tatgctatat
    26821 tgtcaatatg atattgaaaa aaatacacct tcttgctatt attagagcaa acggaggtat
    26881 gatatggatt tcgcaacaac actaaaaacc atacgcaaag aaaaaggata tacgcaaagt
    26941 tcccttgcaa aagaactaaa tgtttctcaa aatgctgttt ataattggga aaataaaaaa
    27001 tgtgaaccta gtatcgaaat gataaaaaaa attgctgatg ttttagacgt atcactgtat
    27061 gacttaatag taatttcaac agatctcacc atttcaagtg aggagactgc gaaaatggcc
    27121 ggggttattc tcgatcaaat tcacgataaa aaagtgcttt ctacttctgg tgaaatatac
    27181 ctaaatcgct actattcaat gctgaatgat acagggaaac aattagctgt tgattatgta
    27241 gaaggtttaa cagaaaaccc taaatacacg ttaaaagcag ataatgacga aagtacagat
    27301 tatcatgatt tatcgcaggc agaaaataat ttatatacag cattagataa atataaaaag
    27361 aaaaaataac tatctgagta tggccggcat cctcatgcga ccacatcaat tttgaagctg
    27421 tgtaaatttg tgcaccttcc ggtcagacta agctgccttt ttctagttgc accagtgcaa
    27481 ccggctgatt tttagaccat ttcattttgt aactgacaga accttgacaa tataatatac
    27541 ttacccgggt agtcggggga cgtgttctca tctgatccga gccttacgga aaggatgatt
    27601 attatggttt catggaatga cctgtttact ttcgtcatca tgctggtggc gatactgacc
    27661 tacatagaga cccaaagaaa acataagaaa tagccgtcct gaccctggaa agtttaggaa
    27721 cggctatctc tattttgctt agtattgatt ttcgccggat cgggtgaagt gcactcacct
    27781 tccgactacc ttgttaagta tattatacgc aatataccgt ggtttttcaa ccataaaata
    27841 aagtcgcccc cggcgccaac caggagcgac aaaggaacta catcgggaaa cccgatacag
    27901 tcaacctcac caagctgatt gtatcatttt cccggtgaaa ttacaaccca ccgggcattt
    27961 ttatgccctt ttttacgaaa ggatgataca gtatggcaag tattcgaaaa agaggaagag
    28021 gataccagat cacagtgagc aatggccggg acattcacga taaacagatc cttgaaacta
    28081 ccacatggat cccggatccg gacaagacgg agaagcagaa ccagaaggac ctggacgcat
    28141 tcgtcataga ctttgagcgc aaggtaaagt ccggtatgtt tctgcagggt gagaagatca
    28201 ccttcctgga gttctcagaa cgttggatgc aggactatgc cacactgcag ctggatgaga
    28261 ccacaacatc gatctatgag atattactgg atactcatat cctcccggca atcggacacc
    28321 tgaagctttc caagatccag ccggtacata tgaacaagct ctacaaggaa atgagtgagc
    28381 accggttaaa cgggaaggac ggtggctatt ctcccaccac gatcaagcgt gttcacgctc
    28441 tgatctcttc catcctgtcc acggctgtcc agtggaatgt tatactctct aacccatgtg
    28501 tgagagtgaa gccaccgaag cagctccggg acacatcgga cattcagtat tttaccgagg
    28561 aagagactgc tgccttcctg cagttcctgt cagacggtac cgaagccgga gagatcaagc
    28621 tgcagtataa catcttctac cagattgcaa tcttctgcgg tctgcgccgg ggagaatcca
    28681 ttgccctgct ctggtccgac atagactttg aagcaaagac catgagcatc accaagagca
    28741 ccagcatagt caagggcaag gcggtgacca aatccaccaa gaccaagtca tcagtgcgca
    28801 ccataagcat tccagaccat atcctggaac tactccggga gtaccgtgca gaatatgaca
    28861 gctaccgtta tactgtcggc tccctatggg ctggagacga tcatattttc attcagtgga
    28921 acggagcgca gatgtaccca tctacaccct ctgccacatt taagaaactt atccacagtt
    28981 acaatgcggc acatgaggat aagctgccgg acatccgtct gcatgatctc cggcatactt
    29041 ccgccacgct actgatcagt cagaatgtgg acgttaagac cgtatcaagc cgacttggcc
    29101 atgcacagac cagcaccacc atgaatattt acgctcacgc actcaaaaaa aaggacgtgg
    29161 cagcggccga agccctggag aatctgctga taaaacgtgc ataatgttag tcagatatta
    29221 gacaaaatga aaagcactcc attcactggg gtgctttcta aactctgaaa ctattgattt
    29281 tactgcattt ctacaaatga gacacggggg attcgaaccc ccgacaactt gattaaaagt
    29341 caagtgctct
//
LOCUS       DAWDHD010000013        29036 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_132_length_29036_cov_13.396915, whole genome shotgun sequence.
ACCESSION   DAWDHD010000013 DAWDHD010000000
VERSION     DAWDHD010000013.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 29036)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 29036)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..29036
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132_length_29036_cov_13.396915"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         7365 a         7585 c         6220 g         7866 t
ORIGIN      
        1 caaacactca acatacaaca acatgaaaga aggagatttt cttatgaagt atggtcattt
       61 tgatgatgtg catcaggaat atgtcatcac cactcccaag actccgcttc cctggatcaa
      121 ctacctgggc tgcaatgagt ttttcagtct gatatccaac acctgcggcg gatatacttt
      181 ctataaagat gcaaagctgc tgcgtctgac ccgttatcgt tataataacg taccggccga
      241 catcaacggt aaatatctgt acattaagga cggagatacc gtatggaacc ccggctggca
      301 gcctacgaag actgaactgg actcctacga gtgccgccac ggtatcggtt acagccgctt
      361 cacctcttcc aagaacggtg tcaaggcttc cgtgctctcc ttcgttcctc tgaacgataa
      421 ttgcgagatc tcccagctga ccctgaccaa cggtagctcc gaggacaaga agctgtccgt
      481 attctcttat gtagagtggt gtctgtggaa cgctgacgat gatatgaaga atttccagcg
      541 taacttaagc accggtgagg tcgaggtaca ggattccacc atttatcaca agaccgagta
      601 ccgtgagcgt cgtaatcatt atgcgatcta ttccgtaaat acgaagattg acggtttcga
      661 taccagcaga gatgctttcc tgggtgctta caggggggct gattctcccg aagctgtaga
      721 gaacggcaag tgcacaaatt ccatggcaag cggctggtct cccatcgctt cccatcagat
      781 caacgtggat ctggcagccg gtgagaccaa gacctttatc ttcgttctgg gctacatcga
      841 gaaccccgaa gacgagaaat gggaatccta cggtgtgatc aacaagaccc gtgccaaaga
      901 aatgctggca aaataccaga ccgatgcaca ggtagatgag gcttttgcag cattacaggc
      961 ttactggaaa cagctgctgg cacgctacac ggtagattcc gctgacgaga aagtcaaccg
     1021 tatggtcaac acctggaacc agtatcagtg tatggttact ttcaacatgt cccgttccgc
     1081 ttcctattac gaatccggaa tcggccgtgg tatgggtttc cgtgattcct gtcaggatct
     1141 gctcggtttc gtacatctga ttccggaccg tgcaagagag cgtatcattg atatcgcttc
     1201 cacccagttc caggacggca gtgcttacca tcagtaccag cctctgacca agaaaggcaa
     1261 ctccgatatc ggcagtggct tcaatgatga tcctctgtgg ctcattgcag gtaccagtgc
     1321 ttatgtaaga gagaccggcg atacctccat cctgacccag atggttcctt ttgacaacga
     1381 tatgagcgta gctgctcctc tgatggatca cttaaaacgc tccttagact atatcatcaa
     1441 ccacaaaggc cctcacaatc tgcctctgat cggccgcgcc gactggaatg actgtctgaa
     1501 cctgaactgc ttctccgctc atcccggaga atctttccag accttcggtc ccagtgaagg
     1561 acccgttgca gaatccgtat tcattgccgg tatgttcgta aaatacggcg aagaatatgc
     1621 tcagctgtgc gagctgatgg gtgacgatac cgaagcagcc cgcgcacgta aggaagtaca
     1681 ggcaatgtac gatgcgatcc tgaaggatgg ttgggacggc gattggtttg tacgtgctta
     1741 cgatgcttac agccacaaag taggttccag ggaatgccgc gaaggtcaga tctacatcga
     1801 gcctcagggc ttctgcgtac tggccggtgt cggtgtgaag gaaggacttg ccgaaaaggc
     1861 attgaattct gtaaaagaga gactggatac caagtatggt gtcatgatct tacaacctgc
     1921 atacacggag tatcacctcg aacttggtga agtatcctct tatccccccg gatataagga
     1981 gaatgccggt atcttctgcc acaacaatcc ctgggtatcc atcgcagaga ccgttctggg
     2041 cagaggtgac agagctttcg agatctaccg taagacctgt cccgcataca ttgaggatat
     2101 cagtgagatc catcgtaccg agccttatgt atattcccag atggtagccg gtaaggatgc
     2161 aaaattctac ggcgaggcaa agaacagctg gctgaccggt actgctgcat ggactttcgt
     2221 aaatatctcc caatacatac tgggtgtata tcccactcat cagggattgt ccgtagatcc
     2281 ttgtattccc aagggcttcg gtgatttctc catcacccgt aagttccgtg agggcatcta
     2341 tcacattcag gtgaagaacc ctcagaacgt ggaaaagggt gtcgtatcca tgaccgtgga
     2401 cggcaaggca gtagacggac acatcattcc ttacgaaaag ggcaaggaag agtacaacgt
     2461 agttattacc atgggttaat aaacctgatg tacatagttt aaaacgattt ctatcctcct
     2521 taaaaccccg gcagttgttg tagctgccgg ggttttgcat tggatattcg tagtttgcgc
     2581 tacaacatct acaccataag gacaagcagg ccgtaagcag acgtcggtac ttattgaccg
     2641 tattggggtc aattatgtac cgctacgttc tgcgccagag cgagagcgtg cctgcggttc
     2701 ttatggtgta gatgttgtgg acgtttttgc ggcttccttc cgggactcga tcacgtgccg
     2761 gatggctgtc aaatgatatt attttctcca tttttctgaa gcagcttccg gtatgtggca
     2821 tccattccca gtgctcccag cggggctgtg ataaggatgg ccagcacagc cacggacaga
     2881 acgatcttgc cgcaggacaa tcccatcgat aacggcacag agccgatagc agcctgcact
     2941 gtggccttcg gcagataggc tatcacgcaa aacgtcctct ccttcttatt gagatccgtt
     3001 cccaacatgc acagtgctac accggcggaa cggaacagca gcgccagcag gatcatcccc
     3061 accgctgccg gtccagcttc catagtatag cggatatcca ctgccgcacc caccagcacg
     3121 aacagcacta cctccgctgc cagccacagt ttaccgaatt tagcagacag tctgccggcg
     3181 acttccctag gtgcccgtat tttcagcgcg caggccatgc tcaccaccgc caacaggccg
     3241 gaaaaggcca cagttccctt taacagcgtt tccagcgcca ttaataagaa agataccccc
     3301 atgatgataa taactttcat gctgttgcgc acataactgc cctttcggta caacagttca
     3361 aaaagctgcc ccagcagcca acccaccaat gcaccaaaca gcacacccgt caggatcgat
     3421 accgggatat tcacaaaatc cagggcattg acctgtccac cctgtgccat tccaacaaaa
     3481 gtggaaaaca atactatgac atagatgtca tcacaggatg ccccagccag aatcatctgc
     3541 ggaatgcttt tctccgtacc atagccactt tccataagat gtaccatccg cggcaccaca
     3601 accgcaggtg acactgctgc cagaactgct cccatgaccg ctgcatccac tctgctgatg
     3661 ttaaggatca tgggtgcaaa tatcacaaaa gccaacagtt cgcaagtggc aggaacacaa
     3721 gccagcatga ctgcggagcg gcccactttt ttcagatcct tcagatccaa tgataatccg
     3781 gccttcaata aaatgatgat cagcgcgatc tgccgcagtt ccgatgagat attcaggatt
     3841 gacacatcca gcagatccaa cacataaggt cccagaataa tacccgtaag cagcattccg
     3901 ataatacggg gcaggtgaat cctctggcag accgccgcca tgagtaatcc cagtaaaaag
     3961 atcagtccca gtgatgtaag cataaaaatt cctccgtttt ctcgcagcag gacattttga
     4021 attccaacaa aaaaggctgg tgtcccctgc atattctgca gaagtcatca gccaaatcga
     4081 attcgtatgt tcaaattccg gcggttcgaa gcctgcgctt ccgggagaac ctcattcccg
     4141 atgttcttat tatatacacg gtgcccgccc atgtaaagtc ctatattttg caaatatacc
     4201 cgccagcatc ctgtgctcca ttaaaaaata tctgctgtga gatcatcccc gaagtctgct
     4261 gccggaatct caaagtcaac aattccaact gcataggact gtaatacata ctggccggat
     4321 tcaaatacaa agccgtcctc tgtaatgtag aatgtagggt ctgcggggcc attatccgta
     4381 cctgcaatgt ccggcccata aatttcctga tagatagcat tcaaatcttc cgtaccatcc
     4441 aacaccacca gtttgatact cttctcgtaa tcgttaaaga agcagtcctc ctttgcctgc
     4501 atttccgctg cctttgctgt caccagttcc agtgctttgt cgcggaatcc gctccccaga
     4561 ctgtcaaaag taattctctc tccggtctgc gtataatagt tcatatagta caaagtatac
     4621 catccatgag ccccctgatc atacccttcc agcccccacg acaggctgat caccttatca
     4681 tccgcgcgga tcacatggaa agtaagatcc tcatagctct gctcatacac cggcatatcc
     4741 tcataaagtg taccaaactc cccgttcttg attccttcta caaaactgtc tacatattca
     4801 tccagctctt tctgaatctt cgtctgcacc gtctcattgc ccggaatcgt cactgtaact
     4861 gttggatagt aataggtata acttctggtc ttgtcttcgg gatcactgct accctcgtga
     4921 tcctctgtgg taaactgcac ccattcactt ccctgtattt cactctggcc ttctgtcggt
     4981 tccgtctgtg tgggcacttc cgaagtctcc tcagtcccgg ccgtcgtctc ttccgtcgtc
     5041 tgaccgctct gtgtctcctt gatgctgtct ataactgtct ccatctccgg atccgtcacc
     5101 atctcccggt ttccgcagga tgtaaaacct aacagtgctc ctgcaaatac tgctgcaaaa
     5161 aatatttttt tcataatcct cgcctcctga tgccagtata tctgccgatt gcatcatatc
     5221 tgcatcattt ttataatttt tctcgatttc actttttaaa tatgatagac aattcccata
     5281 ttttgagcta tagtaaagcc aaatctgggt atgattaaaa ataacgatac acatactata
     5341 aaaatcaacc cgaggaggac tgttatgaaa atattatttt atgataccaa aagctatgat
     5401 aaagaatctt tcgaggcaac tctgaaaaaa tatcccggca tccagattga ttacatccgg
     5461 tcggatctgg atccccgtac tgctgctctg gctgacggct tcgatgccgt ctgtgctttc
     5521 gtcagctcca atgtggacga ggccacatta aaaattctcc acgaaaaagg tgtccgtctg
     5581 gtcctgctgc gttgcgcagg atacaacaac gtggatctgg aaaaggcagc tgcatatgac
     5641 atatgtgtca tgcgtgtccc cggttattct cctgaagctg tcgcagagca tgccatggct
     5701 ctggctctgg cctgcaaccg ccgcttatac aaagcctata acaaggtccg ggaaaatgat
     5761 ttcagcctcg ccggactcat gggcttcaat ttctatggta agattgccgg aatcatcgga
     5821 accggccgga tcggtgcagc tatgtgccgg atctgtaacg gtttcggcat gaaagtcatc
     5881 gcctacgatg tctacgagaa cccggatctg aaagatttcg tcacctatgt gaccttagac
     5941 gaattactgg cgcaaagtga tctgatctcc cttcactgcc cgctgatgga ttccacctac
     6001 cacctgatcc agcttgacac catcaagaaa atgaaggatg gcgtcattct ggtaaacact
     6061 tcccggggtg ctctggtcaa gaccgaggat cttatcgagg gcatccgcct gcacaagttc
     6121 gccggggtcg gtctggatgt ctatgaagaa gagacgaaaa acgtcttcga ggatcgttcc
     6181 gatgagattc tggagcattc taccaccgca aggcttttgt ccttccccaa tgtcatgatc
     6241 acatcccatc agggattttt cacaaaagaa gcactggaag ccattgcaga gactaccttg
     6301 cagaatgctc tggatttcga aagtggaaag aagagcccca atcaggtgca ataaaatatt
     6361 tccaatcgag taggctgact catcgtatac acaaaaaacg gatggccctc ccaagccatc
     6421 cgtttttctc gtttttcagt ttcccgtctt gtcttttttc tctatctctt acagttttta
     6481 cgatcattcc agattcagtt tctgaccaac tctgatcaca ttctggtttg taatctgtac
     6541 atttctgcga agcaattgtg ccagagtcat gtgatttctc ttagcgatcc tggaaagcgt
     6601 atcaccactc tttaccgtat aatcgccctc tgtataccag atgctctcgg aaggtctgcg
     6661 gtcagtaaca gttaccagat aattgccgcc gcgcttcaat gcgaacatgg actgtccggt
     6721 ggaagttacg gtaaaggatc cgcaatactc caggcgtccc ttctctgtat tatagcgata
     6781 caggtttgca ctcttgccgg aattgtcctt gcctacattt acatgaatgt tgacattcac
     6841 tccgaagctg ccggtatctc tgatacccag ctgtctgttc acggttccgg actttgcgga
     6901 taccacattt gccgggattg tgttgctcgt ctggtctact gtcagatcaa tattctgaat
     6961 ctttgacaga tctttattct tcagatcctg tccactgatg ctcagtgcca caccgtttcc
     7021 gctgtggaat gccaccgtca gcttcgtacc tttaatggta ttcagtacat tctgaggcac
     7081 cttgacttca ccgctgcata caaagttcat gttgacactt gcaatagcag gatttttcat
     7141 catctctgcc accttatcct gtacagagtt gcttacatcc gtccagtcta ctgcaggtgc
     7201 tgcagaggaa cctctgttgt catcatcatc atcgtcatca tcgttgttca ccacatctgc
     7261 cgctaccgta aatacagcag aaggggttcc ggtcattctg gctccaacac cttccggtac
     7321 tgcggaagtc actgccagga aaccgttcat attgatggta ccgtccgtat ttctgcgaat
     7381 gcctccgtgg attgcactgt tcatatccat gttctggaac cattctgctg tagctgctat
     7441 tccttcactg ttggtagagt tttcgcctct ccaataatag ttcattgcat tttcgtaggc
     7501 actggtctcc tgagttcctt tcggtttgaa ctgttctgca accgtctgcc ctgctgcatt
     7561 gctgctatcc ttaaaggaca ggattccttt tgctataaat gcagtattct ttgcgtcatt
     7621 tgtatagaat gccacgttaa aggactcatt gttataactg gtagaagaat atgcctcaat
     7681 atccggacag ctgttggaat caataccctt tgccttattg ccgaatgata cactgttgcg
     7741 caaaatatga tgtccgctga tgctggaacc acccatcttg aagccgttac cgttaccggc
     7801 atctacttcc tgtccgttct catccagtac ataaccgttc ttgaatgcca cgcagttctg
     7861 aatgaccacc tgaccgatag ctccggtctc taccttggcg aacagatccc aaccgtcatc
     7921 ggcattgtaa gctgcgatac atccatcaaa tacattaccc tcaccaatcg tcagcttagc
     7981 tgcaaatccg tctgcatcct catatcccgc atctgcgttc agatatgaag tacagttcag
     8041 gatcaggttg tgggacggcc agtcttccca gttatctgtg ctcttatatc tgctgatctg
     8101 aagaccggta ttaccatttt tgtaggtcac tacgttgtcc acagtgttgt agcttccgga
     8161 tacctgaata cccttctgcg catttgcgga tctcgtaaca tcaaatccct ggaaatacca
     8221 ataattgcct gccaggatca tacccgcaca tcttccctgg aaatccagta ccgggcgtgt
     8281 ggcagcttcc ggatctgcga tcatgtaaat cttcgcatct gcggttccgt taacgcctct
     8341 ttctacaatg actgtcttgt ccagtgcata agttccgccc tttaacagga tcgtccgtcc
     8401 gggagcagca ctcttcactg cggtataaat atctacagga tcatccttgg caccgcttcc
     8461 atagcttctg ccgtcgggac ttacataaat ataacttctg tcttttacat tataggttac
     8521 gttgaaatca aatttcacag tatcataact gcttaaaaga gaatactttg caggcttgta
     8581 atctgcatcc ggtgtgaact ccactgcaaa agtattgctg ccctgctcca gaaccgtatt
     8641 gacacggaac ttggtcttgg catccacatg ctgtccggat accactgcct ctcccttgct
     8701 gttcgtaata ctcagaacgc catcggcatt gccgtagtat accatctcat agtttgcatt
     8761 gttggcaacc ttggcagact caatgctgaa gttcggggtt acataagtaa tcggtcttgc
     8821 ttctgcaggt gcatcgtttt ccggagccac tgtggtcagg ctcatattgc tgaagcttac
     8881 cttagcactt ctggaagcat agaaacctac ataaatattg ttttcatcca gtctggtcag
     8941 ttcatcaccg ttgtcaccgt ggtaatattt gttggtcacg gtctctccgg tagctgcatt
     9001 ggtatagctt aagaaatatc cggtattgtt tctctggatc gtcatatgga aggttgtctg
     9061 tggattgaca tctgtgatat cctttaatgc tgcctcatat ccgcctacca tattataagt
     9121 tccggcaccc tgcccacttg ctacggcact ggtctcaagt gttctcatgg ccgtctggaa
     9181 atcatctact acagtgtctg ccgcaagatt ctccggtgtt acaccgattt tctcctgaga
     9241 tccgacaccc agcttcatgg tgtacttatc tccggcatca gatacctgac cggcctctgc
     9301 atcccagaaa tattccacct tggtcgcact taccatataa gagttgttcc agaaagaatt
     9361 accggaaccg ttctctccca cacggtctgc tgccatcagg ccgaagcctt cctgaccatt
     9421 ggtaaacttc caactatcta cggttacatc tgcggacagc gtaaagttct ctgtctcagg
     9481 atcaatggtg gtatagtaga atgccacacc atcggtggag ttcggtacca gcttaccctt
     9541 gctgttcata ctccatactt tcagatcctg ggtatcataa cttccgctga aaccattgtt
     9601 cttgccgcct tctgacatcg tatctacgct ggaaccgaat gcggaataat tccaggtctt
     9661 tactgcatct tctgttatcg ttaatttcac ctctgccggt ttggaaatat catttcctct
     9721 gactgctgca actccgaaag tatattcctg tcctacggtc agtcctgaaa ttttctgtaa
     9781 taactttgtc acaccctctg catatacctt gccatccaca tatactgtgt agttctccgc
     9841 ttccggaaca gcctgccagg tcagtttcac attgccatga cccaggttct ctgcattcgg
     9901 accggcaggt gctgccatgg gtaatacaaa ttctactgct gttgtcttat tacttgcttt
     9961 tgtattctcg ccttctccta tcagttttgc ttcaaagctg taggtgccgg acgctgcagg
    10021 ctggaactct gcatttccgg aagttccctg tgcagtaaca gattttgtct gcactgcatt
    10081 accgtcagca tcatacattg ttacatccag agatttcgca tagattccac cgatctgtgc
    10141 agtgtacgga acggtgatgg ttccgtcctt gacagaaggc gttcccagaa cgggagctgc
    10201 tatgtcatcc cagcttacgg aagatctgta atttacggtc agagcataga tcataatctt
    10261 actcccctgt gcatataagt aataagcgcc tccctcttcc acttcataag ttttgctcat
    10321 taaacccgat tcagaggtaa aagtgtcgat cactttaggg ctcccctcta catcctgtaa
    10381 aaagtaaatg acttttccgc ttttaaagac aacattgatt ctgccatctt tttccgcctc
    10441 cagttttagt gctgcaccac tggtaggaat tttccctttg ctcggatcag gcttcgtatc
    10501 tccctgtaca tatcctgcgt actgtacccc atctaaactg cctgcactgg atttgaaatt
    10561 catagtttca aacacagaaa ttccgccatc atacgttgtt cctgctgtca gacccttgct
    10621 cagatccaat tcatacaggt ctttttccgt tccactctgc acagcagcca ttgctgcatt
    10681 cgcatcctgt gttgtcgaag ctgccgcaat acctttttcc tctcccaaca cttctctggc
    10741 tgttttcgca tttccgtcag aaaccgtctc acttacctgt tctaccacag gttccgtttc
    10801 ctctgcctgt acagagatgg ggctgattcc cattcccgac agcaccatgg ctcccgcaag
    10861 aagcaatgcc atcagtcgtt ttctctcttt tcttcctctc ctgcctaaca ttcccatacc
    10921 tccacttagc atgtgttata aacgattaca tttgtaagct cttacaacac tatgtactat
    10981 atattagcac tattgcttca cattagtaaa ccatttttgt gtattttgaa tatttttgga
    11041 agttttacat atattttttg agcaatattt agttattatt ttttgcgcaa gcctgtactt
    11101 ttctcaaaaa tgcacaattc cgccattccg tgttcatttc tatcaagtaa gcgcttccat
    11161 tatcataacg ttctctatcc attttatttt gactattgaa ggcgtcttgt gcttggcaaa
    11221 agttatataa agttatataa taggtacaaa gttatataca cttatataaa gttatataag
    11281 attttaaaag ttatataaac ttatataaga aaaggaggtt ttcattatga gtaccaatcc
    11341 gtttacgtta tcttttggaa aaaaaccgtt gcaatatatc tcccgtctca cggaaacaaa
    11401 ccaaattctg gaaagctttt gtgccgagat tccatccaat cagatctaca tgatcaccgg
    11461 tgtccgtggc tctggcaaga ctgtaatgat gacaaatatt gcttcggaat tacgaaaaag
    11521 agaagactgg atcgttgtgg aactgaatcc taccagagat ttactgcaaa gtcttgctgc
    11581 gaaaatctac agcattcccg aattacacac gctgttcatt aatgcaaaac ttgacttttc
    11641 tgcttttggt ctgggagtat ccattgaaaa cgctgctccg gtcacggata tcgaaaacgc
    11701 tctggaactc atgttgaaat atatccagaa aagtgaaaaa aggttgctga tcagcgttga
    11761 tgaagtgacg aattccgaat atatccgaat ctttgcaagc tcctttcaga tttttttacg
    11821 aaacgactat cccatctttt tactgatgac gggattatat gaaaatattt ataatcttca
    11881 aaatgataaa gtactgacct ttttataccg tgcacccaaa ttaatcctgg agccactcaa
    11941 ttataccgct atccgcaaac aatatatgga tatcttttct ctggatatag atgctgccgg
    12001 agaactggct tcactgacca aaggctatcc ttttgccttt caggtacttg gttatctcta
    12061 ctgggaaaat cgcgataaaa agactttgga acagattttg ccggaatatg accaatatct
    12121 ggatgaatat gtatacagta aaatatggtc ggaattatcc gatctggata aaaaaatcgt
    12181 aactgaaatg tctctcagcg gagagactca ggtaaaagct ctccgtgagc gattagatat
    12241 gaaatctgaa cttttctctg tctatcgtga gcgtttaaag agaaaaggtg tcattatttc
    12301 caaagaatac gggaaagtca cactggcctt acctcgcttt gatgagtttg taaaaataca
    12361 acagcttatg taactgcggg acgcagcgtc tattccacgc cccgcagcac aactttcccc
    12421 acatatttcc gggccagttc ctccatctgt tccagttcct ctttggaaaa tgcgccgaac
    12481 ggatctacac cgcccggttt cacatcgtca cattccgctg tctgtatcat ccggtacagg
    12541 cagttttcat tctcttcgta attctgaaga taatacgcag ggcatccggc gatccattta
    12601 ccgattttct caaattccgc cacggtatga attcccttta ccacggtagt gcggaattca
    12661 taggggatgc cactgctttt caggatgccg atactctcct cgatgcggga gatatctata
    12721 tgcggcagtc ccgccgcccg agcataatta tccctggaag ccttaatatc catcgccaca
    12781 taatcaatca ggtgctcctg caacaattcc cacaataccc ccggcatata cccgttggta
    12841 tccagcttaa tgggatactc cagctccttg atcttttgca ggaacgcccg aaggtcccgc
    12901 tgcagagtcg gctctcctcc ggtgacacag acaccctgga gaatcccctg cctttttttc
    12961 aggtaagaga gtacttcttc ctccgaaatg agcggctggc agccgggatc cagcaccagt
    13021 tccccgttgt gacaaaacgg acagcgaaaa ttgcagcccc ctgtaaaaac agtggctgca
    13081 atatgttccg gataatctaa taaagttgtc ttgttaaaac catgtatctg catagtctaa
    13141 gtcctcggca tagtggaatc ccgcaccacc agttctccgt cgaacacgca gtgctggtgc
    13201 ttcgcctttt tcccgatgat gtcgaacagg atccgggccg tctctctcgc catgctgtcc
    13261 accggctgct ttaaagtcgt caaggacggg ataacatatt tcccaatttc cacaccatca
    13321 aagcctgcca gagaaatatc ctccggcacc ttgtatccgg catcagacaa ggctttccct
    13381 gcaccgattg ccagcatatc ggagattgcg aagatcgccg tgcacggaat ctgcttctcg
    13441 aggaattcct tcgtcaccat atagccattc tccagggagt aatcttccaa atcactcctc
    13501 ataggcagga tcagttcttc tcttacttct attccgttat ccttcaacgc ctttttatag
    13561 ccctccagac gcagcttacc gatggaagca tccgttttgc gggcacagag gattgcaata
    13621 tccttatgtc cgttcttgca cagatagtcc accatcttgt agctttcctt ggtatcatcc
    13681 accgctacgg aggaataggt gttgcggctg aagcccttgg gcagcattcc cgtggtactt
    13741 aagataaacg gcacatgaag ctgtgccagc ttttcctcac tgtgcaccag ataaccgccg
    13801 aggaagataa tgccgcgaag tcttttttcc ttgaccagct tcagtgccac ttccacttcg
    13861 tcctcatcat agtctacatg ctgcaatacc atggcgtaag atttttcctt gatctcttct
    13921 tccaatacct tgatcatctg gctgaagaaa gagttgttaa tacctttgac cagtactgca
    13981 attgccttgg catcctgacg tttcaggttt ctggcactgt tgttgggtac gtagttattc
    14041 tctttgatca cccgcatgat catttccttg gtctcaggat tgatgtccgg gtggttattt
    14101 atggcacggg ataccgtgct gacaccgaca ccgcagattc ttgcgatgtc tttgatcgtg
    14161 atttcctcca tggagcacaa acctttccaa gcctacggac ctatctgccg tacagcttcg
    14221 tcatactgcg gatccgtcct gcaagctcct ttgtaaaatc tccgttcccc attctccact
    14281 tccagttgat gcccagtgtg gaaggaatgt tgatgcgggc ctccgatccc agtccaagat
    14341 aatcctgcat gggaatgatc gccagatccg ctacgctgga cattgcggca cgcacaaatt
    14401 cccagggaag ctgctctctg ggaatcggct ctccgtccgc atttttcaga cgcagatagt
    14461 cgtcacagag ctttttgtca gctcccttca aggtgtcata ccaccccatc acagtatcat
    14521 tatcatgagt tcccgtatat accacgcaat tatgtgtata attatgcggc agataatcac
    14581 tttcctcacg ggaatcaaat gcaaactgca aaattttcat acctggataa ccggatttct
    14641 tcactaattc tatcacagat ggtgttaaaa agccaagatc ttctgcaata acttcttttt
    14701 ctcccaattt ttccttcatg gaacggaaaa tatcatatcc cggtccatgt tcccagtggc
    14761 cgtgctccgc agtgggttcc ccatagggaa tcgcataata agcatcgaat ccacgaaaat
    14821 gatcgatacg taccacatca tagcgttcaa agcaggctgc cagccgctgc atccaccagg
    14881 cataaccggt ctccttgtga tgttcccagt tatacagagg attgccccac agctgtccgg
    14941 tggcggaaaa cgcatcggga gggcatcccg ccactgctac cggctcacaa ttctcatcga
    15001 actggaacag ctccggattc gcccaggcat ctgaactgtc aaatgccaca taaatgggaa
    15061 tatccccaat aatcttgatg ccgttatcgt tggcataggt ctttagtttc tcccactgta
    15121 cctggaacag atactgctgg aactggtaaa acataatctc tttcgcatat tttctgcgat
    15181 atttgtccat ggcagccggg gttctcagct tgatatcctc ttcccaggct gcccagcaga
    15241 tgccgcggaa accatccttg atggccatat aaagtgcata atcctccagc cagtacgcat
    15301 tcttctgtac aaactcctga aatctcgcat cttcttcaat atgactgtta tcaaacgctt
    15361 ccttcaacag gcggaaacgg ctcttataga ttttgccgta atccacggaa tggatatccg
    15421 tgccgaagtc ataggtattg cactgttctg cggtcacata gcctctcgcc accagatcct
    15481 cgggatcaat aaagtaggga ttacccgcaa aagtggaaaa agactggtac ggagaatctc
    15541 catagctggt gggtcctaat ggcagaatct gccagtaggc ctgaccggct tcttttaatc
    15601 tgtcaataaa tttatatgct tccttggaaa agcagccgat tccgtagcgg gacggcaggc
    15661 tgctcaccgg cagtaatatt ccgctttttc tcatattaat aaccacatct ttctattttt
    15721 tagcccttaa ctgcaccggc taccacgccc tcgataatgt acttctgaca ggtcagatag
    15781 aagacgataa tagggattac ggacagtacc agcagtgcca tcatggcacc cagatccttt
    15841 gcaccataag aacctaccag gtattgtact acaaccggta tggtcttgta attagtactc
    15901 aggccgatca ccagataagg cagcagatag tcgttccaga tccacatggc attcagaatg
    15961 gctacggtga tggctgtggg cttcaggatc ggcattacga caccgaagaa tgtctgcaaa
    16021 gggttgcatc cgtcgatcat ggctgcctct tcgatgtcca gtggaatcga tttgatgaat
    16081 ccacagaaca tgaacacgga caggccggaa ccaaatccca gatacagcag caccattccg
    16141 ggagggttgt tcaggtgggt catattagcc agcttagaca tcgtgaacat aaccatctgg
    16201 aaaggtacga tcatggaaaa tacaaacata tagtacaata tgttggtcac accggttttt
    16261 actctggtca ggtagtaagc ggtcatggaa gtaaacagaa tgatcgctgc cacggaaaat
    16321 accgtgataa aagcagacca tccgaaggct gtgaagaaac ctgttttttc aataccgttc
    16381 acatagttgg taagccctgc aaaagtctct gcggtaggca gtgcgaaagg attgttactg
    16441 atgaacagtt ttcccttaaa ggagttgatc agtacgaaga aaataggtgt caaaaatgca
    16501 actaccagca cacacaggat acaaaaaatg attacattgg aagttttttt cttagtcatt
    16561 attgctctac ctccttacgt ctggtaagta ccagctgtcc cagggcaatg actgccacta
    16621 caatgaagaa cagtactgct ttggcctgac caacaccttc aaatccgctt ctgccataga
    16681 aggtattgta gatatccaga gccagcatag ctgtcttttt gctgggtgct ccagccgtca
    16741 atgcaaggtt ctggtcgaac agcttaaagc tgttggacac ggtgaggaac aaacatatgg
    16801 taatagatgg cataaccatc ggaattttca ctttgaataa tgtctgtaac gaagtagctc
    16861 catcgatctc tgctgcttcg atcagatccg taggtacatt ctgcagtccg gctacataaa
    16921 tgatcatcat ataaccgatc atctgccagt tcatcagaat gatcagaccc cagaatccat
    16981 aggtcggatt cgctaccagt gttgtctcgt attttaacaa tactgcgttg atcatctgct
    17041 gccagatata tcccaaaatg ataccgccga tcaggttcgg catgaaaaat actgtacgga
    17101 acaggttggt tcctttgatt tttctcgtca atgccagtgc cagaatgaag gcaaacacgt
    17161 taatggtaat gatagataca acagaaaact ttaaggtaaa accgaatgcg ttgacaaaat
    17221 ctttgtccgc aaaaattttg atataatttt ccaggcctac aaattgtgca tttgtaatcg
    17281 tcttaaattt gcaaaaggac agatatacgc ccataacaaa cggtatgata aatgcaaagg
    17341 aaaatgcgat caatgtaggc agtacaaaaa tgggaaaata tcgttttaat gtcttttgca
    17401 taagccttct cctattaatt cctttttaca tgatattccg ggttttacaa acacgggggc
    17461 cgtggtacca ccaccgcccc ctgtgtctga agttatataa gatggattaa gttattgtct
    17521 ttttggcgat ggagcttagt tagctgcggt tgcggccttt tcagctgccc atctctcaac
    17581 tacggttgtc tttacagcat cccagtcgat ggaaccggaa gcatactcca gaagagctgc
    17641 accgaagtca tccttgaact gctggctggg gaaggaagtg aagttccatg ctacagatgt
    17701 cttggagcta tctgccatgt aacgaagaac ttcctgtgcc agaggatcgc tgggcttttc
    17761 atcatcgcta aaggtgttga aaggagcgat gaatccaagg tcgttggtta ctgctgcctt
    17821 accagtctca gaagagaaca gccactcaac gaatgcgata gatgctgcct gatcctcagc
    17881 agaagcttcc ttgttgatgc agaagaagtt ctcagtaccg gtgcacagtc cctggttctc
    17941 ttcaccttca actcctgtat agataggaag gaacttaaca tcctcagcct taacggtgtt
    18001 accgtctacg ccattgatct ggctccaacc ccagttaccg ttctgtacca ttgctgcctg
    18061 acccagagcg aactcagcca tggagtcgtc tacagacttg cttcccagca gaccgggtgc
    18121 tgtgcaggag ttgttgatgt acagatcaaa aatgttcttg aagttatcgg agtaagtgaa
    18181 ctccaggtta tcggtatcgg tgataccctt gtccttgaac tcgtagtata cagggatgtt
    18241 agccagatga gtctgccatc tccagtcttc accgggagtc agggatgtgg aagcgaatac
    18301 gccttcaata cccagttcat ctttcttagc ctgcatatct tctacaacct cttttaactt
    18361 agcgaagttg ttgatctcat ccatgcttgc tgccttagca ccatccagtg cgaagtactt
    18421 ctgcatgata gcgtcgttgt aaatgatacc gtaaccctct actacgtaag ggattccgta
    18481 tacgccaccg tcttcgccgg tgattgccag gctcttatcc attaaccagc tgtacaggtc
    18541 tgtatccttt aagtctgcac agtagtcttt ccagttctga taaccgatag gtccgttaat
    18601 ctggaacagg gtaggtgctt ccttgttagc gatctcagac atcagggtct gctcataagt
    18661 accacttgct gcggttacaa cctttacagg aacaccggtc tcctcggtat attccttagc
    18721 cagcttctgc catacttcgt caacttcggg tttgaagttc aggtagtata cagagcctgc
    18781 tgcctctgcg gtattctctg cggtcttctc tgccgcctcg gtagttgctg ctgcgtccgt
    18841 cttgttgtcg gtagcagggg tggttgctgt tgcattgtca gcatttccgc aaccggtcag
    18901 catggaagct gccattactg cacaaagtaa aacacttgcc agtttcttct tcataatctt
    18961 acctctcctt ttttgtttga acgggaaccc ttcataattc ttgctatact cccgtcaggt
    19021 tgtcggaaac ggtttcggta ttgttcccgg taacgtttcc gttgctgtaa ttagaaaata
    19081 gcacattcac gaactggttg caagtgtttt tctgtaaatc cctggaagtt tgtatattgt
    19141 tatcaaaata cttcttgcag ttttgtgcat tttcacaatg actttataat tcttctgccg
    19201 cttatattct tttttcaaat aattaacccc tcgagcggac tgtctcctac tcgattttta
    19261 tactttgtac accatctcgc ttaaaagctc gatgatgtac attcgccagg tacagattct
    19321 tgtgcaagca caaatctgta cctggctcat cgtatacaca aaaagaggtg ccgcctcatc
    19381 gttctgcagc acctcccggg ttcttttttc tattttattt ttacagatac tttttccaac
    19441 cttccggcag atattttgcc gtgctgcgga gcatatccct taccagctct tccacctgtt
    19501 ctctggaagc tcttcctttc acctgcggat cctccaggaa tgcttccacc accagatcaa
    19561 aatcacacag aatagctgct ttcagaatcc gctcatggtt tgccacatgg ggatccacca
    19621 gctccttgat ctgctccgga agctctccgg catacaacgg gcggatggca tctctctcga
    19681 ataccgcatt ggtctctaca accgcacttt ccggcagatt ggcaatctga tgtccggtat
    19741 tgggaatatt gacattgctg atcttgcggg tcagtccgca gagtgccttg atcagctgga
    19801 taccttcctc cccggtaggc ttcaactcga tctcttcctc accggctgcc agtctcgcgc
    19861 tctttgccag acgttcctgc agatccttct ttctccagtc tacggtagtc agaccgaatt
    19921 tccatctctt taccgtctcg ggatctttca ggtaactgtc acccacaccg ggcatgaatt
    19981 ccaccagatg cctgtcgcct gctgctgcga tcagaccata tttgcggaac agatcaaatt
    20041 tcacaatatg ggcacactcg aaagtagagt tcgcccagtt tttatctgct tctttgtagc
    20101 cgtcttcctt atgctccccg gtatatttcc ggtaaatggg gaacagatcg attcccttat
    20161 aggaagcata gtcgaaccag gtaaaatggt tgatacccag tacattgacc tgaatatcct
    20221 cacgtttcac atccttaagc cccatggtct cttccaaaat accctttaac actttctggg
    20281 taccgaatac ttcatggcag catccaaaag ccttgatctc cggaaaaacg tagtacaaag
    20341 tcttcacgca cagggacatg gggttggtgt aattgatgac ccaggctttc ggtgcatatg
    20401 ccctgatggc ctgtgcgatt tccacataca tgggaatcgt acgcaatgct ctcatggcac
    20461 cgccgggtcc tgcagtgtct cctacggact ggtagattcc cagtcgttcc ggcatatgta
    20521 catccacagc catctcatca aaagttcccg gcagaataga gatcacgatg aaatccgcac
    20581 cggtcaatgc ggactgtaag ctgtcagaaa cagtatagtt ccatttgccc accacatcct
    20641 ctcttgcaga aagtctgttg ccgatgatct cattgtgttt tgccgcctcc gcatcaatat
    20701 catacaggcg gatggttccg gacagttctt catccatggc cagatccgtc ataaatgtcc
    20761 atgcccatcc tctggaacca ccgccaatat aggcgatctg taaatctttt acctttccgt
    20821 ctgcatactc catttttgta tcctccttat gtgtttccgg tccttgggat cctctgctcc
    20881 atcggcccgt actgccttct ctatattgca cattatagcc tatagctgta taataatctt
    20941 gctattattt gcgtattttt tgccattttc gtactatatt gacttttgtt tttatacaaa
    21001 cccatagatt tgtaatataa cctgcgcata ctgtttttca gaatgcagtc taatatagca
    21061 ctaatatagc ataggaaaaa ggagatcact attatgtttg aaacagggga ttttcagaca
    21121 ccggctccac cggaatcagg cgttgcggac aatgctgttg cttcgacgcc tcaggatcac
    21181 tggctctata atgagcttgc agacggattc cacatcgacc gtgttgtgcg ggactataat
    21241 tattccatgg atatccgtca cattcacgaa gaatacgaga tttattatct tttagagggg
    21301 gaacggtatt attttatcaa ccagcagacc tatctggtca ccccgggaac gctggtcttc
    21361 attgaaaagg agcagatcca ccgcaccggt atggcttccg ccggaccaca tcacgaccgg
    21421 atcgttttag gaatcatgga agagcccttt tctacctttc tggcatcttt cggaggattg
    21481 cagctgcaga gttttttcac ggagcagggc agtattctga cactgccaga agaaatgcgc
    21541 ccctatatcc agtctctgtt acaggacatt gcctctgagc tgatccacaa aaagcccggc
    21601 tatcttctgg ccgccatgac gaagctggca gaatttttca ttgccgtgct gcgcctgcag
    21661 cccgagggtc ccgttgtctc atccccggcc agacattcga acccgaagta tcaaaaggtc
    21721 gatgaagtgg cgcactatat tgtagaacat tgtactgagc ggatctctct ggaggatctc
    21781 gctgggcggt tctatatcaa caaattttac ctgagccgta ttttcaaaga agtcaccagt
    21841 tttaccatca atgattacct gaatgtcaac cgcatcaaaa aagcccaccg gctccttctg
    21901 gaaacggatg acagcatcac ttccattgcg gaacagttgg gctatgaaaa tattacttat
    21961 tttgaacggg tgtttaaaaa ataccggaat atgacaccgt taaaataccg gaattattat
    22021 cggtcggaga cagagtgacc tatgaatctg cagctgtctg aacgtatact ccttgtctgc
    22081 ttcatgataa gccgctctac aacagatcac tcagtcttgc gaactgctcc agtgtcaatg
    22141 cctcacctct tacggtaggg gacaggccca tctgtgccag gactgctgcc gcggattcct
    22201 tggtgatgcc tgggaggccc gcatttccca gaccgttcac cagggtctta cggcgctggt
    22261 tgaaggaagc ccggatcagg gcgaacatct tcttctcatc cttcacctgt accggcggtt
    22321 cttcgtagcg ggtcaggcgg atgaccgcgc tgcccacatt cggtctcggg atgaagcagt
    22381 tgggcggtac attcgccacg atttccggct ttgcataata ctgcaccgcc agagacaggg
    22441 cgccgtaatc cttcgtgccg gggcctacct gcatacggtc cgccacttcc ttctgcacca
    22501 tgatggtgat gctcttcagc ggcacacggc tctcgaataa gcccatgatg atcggtgtcg
    22561 ttatgtaata gggcaggttt gccaccacct tgatggggcg tcctccgttt ttctcttcca
    22621 ccagccggtt aatgtccact ttcaggatat cgtcattgat gaccgtgacg ttatcataat
    22681 cctgtaatgt ctcttccaga atagggatca gtgccttgtc gatctccacg gccaccacgt
    22741 tgcccgcgcg ctccgcaaga tactgggtca tggtaccgat gcccggcccg atctccagca
    22801 cgcagtcctc cttcgtgatc tccgcagaag aaataatccg gtccagaacc gtcgtgtcta
    22861 taagaaagtt ctgtccgaat tttttttgaa aattaaaatg gtacttctgc agtaccgcaa
    22921 tggtattctg gggaatgccc aaataagcca taataagcct ccttaataat acttaaaatc
    22981 ctctaaaaaa tgaatacaat catacaacac cagcacatcc attccctgtt ccggttcata
    23041 ctgctccatc agttggaaca gtttcccgtt aaaataccgc agatccacca cgtagatatt
    23101 ctcataatgg gccgtgagca gcggaatcag gctgttggca taagaatcct tgatgacaaa
    23161 cagcgtctta ccgggattgt atcccgtatg aatctcaata aatgcatgat tgtcattcag
    23221 gaaaaatcca tactggttct tggtatccag ataatccggt gcatacagag aatcagccgt
    23281 gtccgcaaaa tcatacgtca ccgatactgc ttttttctcc gtctccggga aatactgtat
    23341 gctatccttc gtccagtcca cattgatacg ggactgcagg gttccctgaa aattctccga
    23401 caccgtcttc atgccgttta catcatacgg gtaggggaaa cgtcccacac tgtccgccca
    23461 ggccagaaaa ccgtaatatg cccccagact ggtccaatga tgatccgtgc gataatagat
    23521 gggctcccgt gcatgctcct gcaaagcgtg atacacatct atataatgct cttctccaat
    23581 agaattcctt accttcgtaa gcagccgttc ttcgtcataa taaggtgcaa atgccggcag
    23641 cttatccgtg agaatgttgt ccgccgttgg taccagcatg actttggcat cccagtggtt
    23701 caccagtttt gtcaacgatg ccacacggga gttctccatc tgttccgtgt attccccggg
    23761 atcgttcact ccgatcagat aatgatccgc tcccagatag accccattga tctccttttt
    23821 ccggaaatga atatctgccc cggtcttaag tcctacccat ttgtcccgtc ctacgaactg
    23881 gtcggacatg tatttctcat acgcatcccc gtattctccc gtaaaaagac tctcccagga
    23941 aaaggcaggg cgggaagcca gcacccggtt ctccgcttcc gaatatactc tgtcactgtt
    24001 ccacagatct gccactccca gaaccagaag taccgcacag accagtccga tggtcaacag
    24061 cgaatttctt ttatcatcca tatgcggttc ctccttcccg gtcagaatcg aaaatacaaa
    24121 aagggattat acgacgcatc cacaatatac gccaccgaca gaagcagcag tactgccgga
    24181 atcacctttt ctcccaaccg gtacagtgcc atggcggggc ctgtcttact gcttttcaaa
    24241 gcatgcacca gtctgctgcc tagcggaaga catgccacca atgcgatcac cagaagcacc
    24301 agatactcat tgcacaaaaa cattgccagc gaattcaccg gccctattcc gttcattccg
    24361 aacatagcct ggagataagc caggatctct ccgggacttt ccagtgcaaa aaacacccaa
    24421 ctgatcagta ctaccgccaa cgtatagatc ctgccgaaca ccttgggcac actttccaga
    24481 atatctccta agaataattt ctccaggatc aggaaaaatg caaaccacag cccccagaac
    24541 aggaaattcc atcctgcacc gtgccagata ccggtcagca tccataccac cagaatatta
    24601 aacagctgtc tcggcagccc cttgcgattg ccgcccagcg gaatatacac atactccttg
    24661 aaccaggtac tcagagaaat atgccatctg tgccagaaat cttttaccga agcagcaatg
    24721 tatggataat tgaagttctc cggaaaatgg aagcccaaga ctgcccccaa tccgatagcc
    24781 atgtcggaat aacccgaaaa atcaaagtaa atctggagtg caaacgctgc gattcccagc
    24841 catgccatca gaatactcat ctgctcattt cccagttccg ttacctgttt ccacaaaagt
    24901 cccaggttat tggccagcag tacttttttg gccagtcctg tcacgaatcg ttttaccccg
    24961 taactgatgg catccagatc ggtccgcctg tcctgcagat accgctccac ctgatgatac
    25021 tttaagatgg gcccggcgat cagctgcggg aacatggtca catagacacc gaactgcaga
    25081 atattacgct gcgcttttgt atccccccgg tacacatcga tcacgtaaga catggtctgg
    25141 aacgtataaa aggaaattcc gatgggcagc ggaagcttta acagcggaat tgatgttcca
    25201 aatactgtat tcaccgtctg tagcaggaaa tcagcatatt taaaaaatcc caggataaaa
    25261 agattgatgc caatggaaca tagcaaaaag atcctggagt gatccttccc tctgtattct
    25321 tcgatcagcc ttccccacac ataatcggat atggtagaaa aaagcatgag gaaaatatag
    25381 acgggctctc cccaggcata aaagaccagg ctccccagga agagaaccag attcttcatc
    25441 ttccggggaa ccaggaaata gcagatcata aaaatcggca aaaaccgaaa aagaaacaat
    25501 acactgctga ataccattca ctctactccg tatttactcc agagtctgtc tgatagcctc
    25561 cagggcaagt tcattctgct cctgacagta agtgatcacg gcctcatcgc cctgttcttc
    25621 cacagaggaa gtgtccgcac ccaactgtac aaagcataca taatcgccta tccgttccac
    25681 tcgggatgcc tgaatcttgc ccagattcat gggatactgc atcgtgtcat tcaccagact
    25741 gtcacggtag gcgttcaatg cattttctac ctcttctacc ttaccatctt ttgcatgaat
    25801 aatcaataaa gtatctacat tgacgctgat catgggtgcc tctcccaggt aggcatcata
    25861 gagctccgca ctgacaccgt aggtctcgtt cagcatttcc ccgggtatct cggaatccgg
    25921 ccagtaattg tctcccagtg catccgtaac tgcctttttt gcactctgta acgctttcgc
    25981 agcgtccttc ggattctgtg cagcggttgt actaccggtc tcgccggtct cctgtcccgc
    26041 tgcggtgctc tccatggtgg actgctggtt gtctgatgca gcaccgttat tatccttgcc
    26101 tccgcaggcg gtcatagaaa gtgccagagc acccataagt aacatacatg ctaatttctt
    26161 tttcataaaa tctctcctct ttttgcaggg atatttccca tgttcagttg cagtatcccc
    26221 tttttgggga tccctattct atcagacgac aatccggcag aaaaagtttc attaaatttt
    26281 caacagccag aacagaaacg gcattgtagc tacggacagc acagtggtca gcgccacgcc
    26341 cttagaggtc agccggtaat caccgtcata ttgctgcgcc agcatgactg tcatgctgcc
    26401 gacaggtgtg gccgtcatga ccagacagac acccagaaac atggggtctg taatgaaaaa
    26461 acgcagtccc cacagaagca gtgccggcat aagcagcagc ttcacaacag aaaagagcag
    26521 cagccgcaca tccgtaaaca gatcctttaa ccggatctgt gccagcgaat caccgattac
    26581 cagcatactt aatggtccgg tgacagctcc gatccgggtg gcagtatctt tgaagaccat
    26641 cggaaccgga agattactga tatacagggt cactgcaatg aggcaggaga tcacccccac
    26701 attcatcagc tgtttcccgt tcagcttttc ccgctgtccg gaggtcttca tcctgttaat
    26761 gccataggta tagatcagca cactgtaaag cagattaaaa attgcagcat ggatcactgc
    26821 ttcaccgccg tacatggcat ccagcagagg atagcccata aagccaatat tggcgaagat
    26881 cgtcatgacc tgataagaac cataatcttc tctgtctgcc cgaagcagtc tcggaatcag
    26941 ctctgcaatc acaaacatga tagcaaaaaa gatcagcgcc acagccagcg taaccaacaa
    27001 tgccgcctta ttttccaatg tttcaggatt catgccggag gcaatgatca gcgccggatt
    27061 gccaatatta atgacgatac cggagatcat cttgccggta ttttccccga atactccgac
    27121 ctttctgcag atgtacccga tcagcatgat caggaaaaaa atcaccattt gttgaagtaa
    27181 aagcacacgc tgccttcttt ctgtatttaa tcaggaaata gtaactattc agaaccccat
    27241 tcgcaaaacc gcgtacatca tagcacatat gtgctctttt tcacaacaaa aatttattgt
    27301 tttccaccct ttttgctttc tttctttaca aaactacgga cagtacgcta tacttgcaaa
    27361 ggactattga tggaaacgac gctgcaaagc agcagaatgt gaggaaatat gagcatatta
    27421 aacgtagaac acctgaccca cggatttgga gaccgtgcta tttttaacga tgtatcgttt
    27481 cgcctgctga agggggaaca tatcggtctc atcggtgcca acggcgaggg aaaatctacc
    27541 tttatgaata ttatcaccgg taaactgatg ccggatgagg gcaaagtgga atgggcgaag
    27601 aatgtccgcg tcggctatct ggaccagcat acagtgctga cccccggtat gacgatccgt
    27661 caggtactgg cttctgcttt tgatttctta tacgatatgg aagcacagat gaatgaaatc
    27721 tgtgacaaaa tgggagatgc cacctcggaa gaactggaac agtacatgga agacctggga
    27781 acaatccagg atctgctgac tatgcatgat ttctatatga tcgatgccaa agtggaggaa
    27841 gtggcaagag ctctgggact gatggatatc ggcctggacc gtgatgtgac agagttgtca
    27901 ggtggtcagc gtaccaaagt tctgctgggc aaacttctgt tggaaaagcc cgatattctg
    27961 ctgctggatg agccgaccaa ctatctggat gcggaacata tcgaatggct caagcgttat
    28021 ctgaatgaat atgagaacgc atttatcctg atcagccatg atattccttt cctgaacagt
    28081 gtcatcaacc tgatctatca catggacaat caggagctga cccgctatgt gggcgattac
    28141 gacaagttcc aggaagtcta tgccatgaag aaatcccagc tggaggccgc atataatcgt
    28201 cagcagaagg agatcgccga tttgagggat ttcgtagcaa gaaacaaggc ccgtgtatcc
    28261 acccgtaaca tggccatgtc ccgccagaag aagctggata agatggatgt catcgagctg
    28321 gctgcggaga agccgaagcc tgaattctct ttcaaggagg gccgcaccac tggtcgtatc
    28381 attttcgaga caaaggatct gatcatcggt tatgacgagc cgctgtccaa gcccctgaat
    28441 ctgtccatgg aacgtggcga gaaggtagcg ctgatcggtg ccaacggtat cggtaagacc
    28501 acactgttga agagcattct gggactgatc ccgcctctgt ccggcgaagt ggagcagggg
    28561 gattatctgg agatcggtta tttcgagcag gaaatgtccg gttccggtga caacagctgt
    28621 atcgaagaga tctggcagga atttcccgcg tttacccagt atgaggtgcg ttccgccctg
    28681 gccagatgcg gtctgaccac aaagcatatt gagagccgcg taaaggtact cagcggtggt
    28741 gagcaggcaa aggtaagact ctgcaagctc atgaaccgtg ccacgaacgt tctgttactg
    28801 gacgagccaa cgaaccatct ggatgtggat gccaaagacg agctgaaacg ggcactgaaa
    28861 gaatacaagg gaagtattct catggtctgc catgagccgg aattctacga tggcctggcc
    28921 acccaggtct gggatctgtc ccagtggacc acaaagctgt aaatggaaag ttaatgaaac
    28981 gtccccgtag cccttggcag ggtataagaa acgcggcacg ctcgcgctct gtcgca
//
LOCUS       DAWDHD010000014        27978 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_140_length_27978_cov_11.782330, whole genome shotgun sequence.
ACCESSION   DAWDHD010000014 DAWDHD010000000
VERSION     DAWDHD010000014.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 27978)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 27978)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..27978
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140_length_27978_cov_11.782330"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         7025 a         7012 c         5662 g         8279 t
ORIGIN      
        1 ccaccaacag ttccatcacg gatattgctc tggaatacgg cttcaacgac agcaattatt
       61 tcaaggatct gttcaaaaaa gtttacggaa agtctcctcg ggaatatcgg aaaaatccgg
      121 attgacatcg ggtttcttat cggcagtctc ctctaagtct tcctcttcga aatgttcttc
      181 cgtctgtttt tcgattttct cttccagtgc ctcccggatt gcatcctgta cgctgtcgaa
      241 cttctccaga aaactgtccc attctttgat gctgaattcc tgattgccga tcatgaattt
      301 cgtctctgta tcgccgttct gaagcttgtc gtagatctcc tgcattttct cgcggatctg
      361 ctccatgaga ctcagatcct ctttttccgg ttctccggcg gttccggccg ggtccgtttc
      421 cgctgctttg tccgcatcgg gcgtctccga agtttcctcg ttgctgtcta cattgtcacc
      481 gttctccata tcatccagtt ctttctccat ctcccgtatc ttcttatcct gtgccagtgc
      541 acgcaggatc cggttcacat cctccggact gaacataccg atggccttgg acagggcatc
      601 gatgttattc cggttaacca gcaggcttcc accgccggac agcggaatgt tgatacaatc
      661 tttctcatta gaggtatctc ccagcgtcag acggttatga tcataatcgc agacgaagac
      721 caccccatta tattcaataa caccgttctc cgccatctcc ccataaggca ccttatcctt
      781 gccttcgata cgacggttca gccgcacccc ccgcgtcacc ggcgccagtg ccttgtcatt
      841 ttcctccgca gcctccaggt tttccgcaaa acgattttcc gcatctgacg tactctgcaa
      901 aactccctca ttcactctct tctgtgtctc atacacgaaa gtccttccat agccgattcc
      961 tgtaattgcc ataatatctc cctctccgac agcgattact gccgcaaaaa acggatggct
     1021 gcctctctca aggccgccat ccgtagcttt ttctccatcc atggtgtctg tattttccta
     1081 tataaataat atcgacacag cccgggggaa actttatagt acaaaactct tgttttttgc
     1141 tgttttttgc atattgccac tttataatag cagtagtaat acctaaagtc agctttagat
     1201 aattttatgc agggagacga aatcctctcc tagacatgaa gctcggattt ttgctttgca
     1261 aaaacaagga ggattatata tgtacagcat cggacaagtt tccgcaatga caaatttacc
     1321 gatttccact ttaagatatt atgataaaga gggatttttc ccgaatctgg agaagcagtc
     1381 cgggatccgg cggttcagtc aggcagaact ggatgccatc cgtatgatcg aatatctgaa
     1441 gcgctccggt ctggagatca aggacatacg acagtttttt gaatgggtag aggaagggcc
     1501 ttccacttat ccgaaccgca aggcaatgtt cgaagccaga aaaaaagctg tggaagaaga
     1561 aatgaagcag ttggaaaaaa acctggatat gctgaaattc aaatgctggt attacgaaaa
     1621 ggccatggag gacggcaacg aagacgaaat tcacgccatg cttccggata aacttcccgc
     1681 tgatattcag gctctgtatg ataacagcca cgaccggaac aatgcctgaa acgaagtctt
     1741 ctgcataaat tttccctcgg aaatgtattt tcatatcgcg taagatatgg tactatatta
     1801 tcagttagtg tatgctaacc tttcgttttc tacataacac acactgaatg accattcgga
     1861 gggagaatat tatgagggta acagatttag atgcacagat acctgatcag atcggtcatg
     1921 cctttggcta ttatgattat ggagaggaaa tcggcatgga ttcctttttc cgcagtaaag
     1981 atgcggtggc agactatatc gccggatatg tgcggatggc atttgctggc ggtatgcttt
     2041 atggcatccg tgagcctggt aggggctata tcgcctataa gctgccggga cagaagctgg
     2101 gctttggagc gagcatggaa cttgtaaagg ctcttttcca ttctatgagc ctgaaggaga
     2161 tcttccgtat gggagttgct atctcgaagg gcggaacctc tttggaggat cggatgaaaa
     2221 aagcgaaaaa gccctgcatc ttcgtcggca tggtctgtgt cctggaggca ttccagggac
     2281 tgggctatat gcgtaaggtg atggagctgg cctacgcaga gggtaaccgt ttacaggtcc
     2341 cggtgatcct ggagacagat gcaaagtcaa aatgcgataa atacagacat cttggaatgc
     2401 agcttgaagg catccgtgat ctgggagcct atggaaaaat gtacgatatg atcaaatatc
     2461 cggattaaaa tggattaaaa tcccatcttt gacactttat agagcaaaaa acagccataa
     2521 ccccagtatt tatcaggggt atggctgttt cctttttcgc atcgatattg tactattttt
     2581 gaaattgtgt tattttttct gcattgtttc tttgataagt gcccgtatat ctgcctttga
     2641 tataaattca taatccgttc tgaatccaaa agcactgtgc aatgcatcgg tcagttttgt
     2701 acgtttgtag gagggaatgc agccactgtc tttggaaagc aggatcatat tcatgtcccg
     2761 aagggttttt aagatttcgc tgcaggcgaa ttcttccccc agtttcttct ccaacagccg
     2821 gtataccaga aggcggatat aagatgtttc tgcaaatgtt gtccaaacgt agttcggtgc
     2881 agagctgttg caggaacaga tagcccacat taaaaaggcg ctcttcgtct aaaggaatac
     2941 atgcgagagg tgaaaaagag acggtaactt tttcctttcg ctggttataa agtaaagttt
     3001 ctttttcagt ttccttttgg gcccagtcca tcatttccgc ttcgttcccg gaaaactgag
     3061 tcagaagatc attgtactgc ccagcttttt atagatgcgg gaagaagttt taccgttttc
     3121 cctgcggtag gactgggtga tataaacatc tttgttgtct ttgctgccag taatattgat
     3181 atacattaag aagtcctcca attaaatcaa ttacaataca gttagtacaa aaaagcacat
     3241 ataaattcat aaaatttgac aaaaaataag ccaggaatgt cttaaataaa ggatttattt
     3301 taaaatgaag aaaaaagaag tgttaaaccc ccgtatcgtt atttccggtt tctctggcgt
     3361 tctttttgtc tgtccgggcg tttttggtgt cctgttatac ctgctgggct ttcgcgattt
     3421 gacgctccaa tcaatggatg gtatagtagt gctgtacgat cgcgtgttgt tggagattcc
     3481 gctgtgtaca ttggaaaact ggtagtctgg agttaggatg ccaccggtgt atgccgagaa
     3541 gctggtggtt aagaaattgg tttctgtaaa gagggaaaga ggttagagga tgcaaaaaag
     3601 tgtgatctat tttaaacgta ctttaccgga aaaggtgata cagttattag tgtccatttc
     3661 aaatgatgct tttaacaatc gtgaggggaa gattgtaggt aatagagaat ccacatattg
     3721 tttatcttac ggtggcgatg aaagtatgta tggctgtctg caattaggga tgctggagtt
     3781 ggaggacaac aaagattttt taaggtgtgt atgtgattgg aagtggattg atgaagaata
     3841 tccggaggag aattacagtg tatggagaat aatggaaaaa tccctaaaag aatagaaccg
     3901 gatagatgtc gtaaacagtt gacttttaat ataccaacag aattgagttt ttgtaggtct
     3961 ggagcagaag gagtgatatt ttatttttat tgatgaaaat tatgtgatta acagggaaga
     4021 attgcctaaa gtccttcaaa gggatattga agaactggag agctattatc atagtggaga
     4081 ttttgtagga tatgattgct gtctggaaac actggaagcg actgtgaagc agtgttgtat
     4141 acatggcagc atcagtggaa agcagttaga cactattttc aggaggtatg gattaaggtg
     4201 atagaaatga taaattttga agattgtcct ataaatgatc gtgtatatgg tggagctgct
     4261 agaagaaaat taggtgttgt ttacgaagga cagaattata ttttgaaatt tcccggtaat
     4321 ttgaaggata agcaacttaa aaatataaaa acgagttaca gtaatgctcc tgtttctgag
     4381 tatataggat ctcatgtata taaattgata ggaatgcctg ttcatgaaac aaaacttgga
     4441 gtgtataaaa acagagtagt ggtaggatgt aaggattttt tatcagaagg agaaaaccta
     4501 atagaatttg aaaaaataaa agtaactttt ttaccgccat ttcttgactc taatggtgaa
     4561 gaaacgaatg ggaccggaac ggatcttcag gaagtactta tgacgattca agagcatcct
     4621 atgttaaatc gtatttctgg aatagaggaa catttttgga atatgtttgt tgtggatgca
     4681 tttataggca atccagatcg taataatgcc aactgtggaa ttataaaaaa agcagatgga
     4741 tccttttcta ttgctccggt atatgataac ggtaattgtc ttaataatag atgggatgat
     4801 gaaacaatga aaaaagtatt gatagatcct ggtattatgg aaaatgagtc ttataaaaga
     4861 aaaactaata tttttatgat aaaagagcac catgtgaaac cgtgtgaata tatttatacc
     4921 aagcggaatg agaaatgcaa tgaagctgtg aaaagactgg ttcccaagat tgatatggaa
     4981 aaaataaaaa gtatgattta tgaaattcca tgtattagtg atgtacggaa aaagttttat
     5041 acatcaatga taagtatgag gtatgaaaaa gttcttttgc caacgtttca ggaattagta
     5101 aatatgcgga gcagaggaat agatatgatg tcactttaac tcacccaaaa cccatgtttt
     5161 ctccaagctt tttttcatat aattaagata tggagtgata tcaccggtat tgtcataatg
     5221 atctaaggat ttataatact ggtcttttgt ttctgagtat actactaaag gtggatagtt
     5281 attgatcatc aaataatagt taataagggt tctccctact cttccatttc catcggcaaa
     5341 tgcatgaata ttttcaaatt tacaatgaat atatgcggct gtttttaaaa tgttatcacc
     5401 cttgtcaggg atatttaaaa tttcgtcaca taattccgct atttcagccg gaacttcttc
     5461 tggtagcgca ccctgatttg atttgccaat tacataatca tgtattttat aatgtccagg
     5521 acgttcacct ttttgccaac ggtgttcgtc atagacccct tgcataagtt gataatgaat
     5581 tttttttata agatcagagg taatggggtc tttatttata attttaggta caagaaaatt
     5641 ataacatttt ttttgatttt caatttcaaa gatggtttga gtatcaccac aaaaattgat
     5701 tacctttcca ttttcaaaga cttctcttgt agcatgatat gttatctgtg gattttctat
     5761 tgttccagag ttataagcaa aagtgatacg aaaattgctg agctgttgat ctaatttttc
     5821 gatgctatct attttcttct ccttccactt gcttatgatg tctgtatagt tcacaaaaat
     5881 actcctttca ttattttgtt aaattgtaaa gtgacatcat gtcacctcca tagaatcatg
     5941 gtgggatatc ctacctgatg ttatacttaa tttcagtatg tgtcaactca aaacaacaga
     6001 taattccgta aaaagaaaag cagatacata gcaacagcca cgcatctgct tttctacatt
     6061 ttatttctgc cccagcttgg agacattgat aactatattc tgtgcctaaa cactacctga
     6121 ctttttaaca ccgataccgt atccacaccg catcactgtc aaattttttc acatcaatta
     6181 attttaactt cgtcacatca tgcagcatta caagtccgtc aaacacggct ggcatgccgc
     6241 ctctgccatc gattccggct ccgctcaaca gactgatctc atcaagaagt ccagcttcta
     6301 aaaatgcagt attaattttc ggtccgccga caatactcat gcgctctacg ccaaactgct
     6361 ctgcaaggat ttctgttgca cgcgcgaggt ctacatgctc ttttacgcag gcaatccatg
     6421 aaatattctg tctgtccaga taatcgagat attcctttga aaccttctca ctggttacaa
     6481 tcagatatgg ttttgccata cctgttgcat ccggtcagag aagttttccg tttgtatcga
     6541 tgacaacttc ataacctgct gttacacgtc cactgactgt tattggtaca ttaatttcat
     6601 caagcgttgt ataataatca tctactccat tcttaaaaca ctattcggat tcctgttatc
     6661 attacatctt tccgctatat acagggaatc cactgcttgc tccataactt tcaatcttct
     6721 tgaagttctt taatacttcc atatcctgcg cactgatttc aaaatcaacc tcagcatttg
     6781 tcttcatatg ctcaggatta gctgtctttg gaagagaaat tgcaccaagc tgcaatgtat
     6841 agcggatgca aagctgtggt accgttactc catacttttc tgccatagct ttgatctctg
     6901 gctgatttaa aatctcaccg tgggcgatgg gggaatatgc ctctacggca atcccattct
     6961 tctggcagta ctccatcagt tctaacggtg tattgctgat gtgcagcagg atctggttca
     7021 ccataggctt aatctctgct gtctgaagca gactctcgat gtcaccgatc tggaagttgg
     7081 agataccgat gacctttaat ttacctgctt tgtaagcatc ttccagagct ctccatgcgg
     7141 ctctgtttcc ctccacataa cgatcctcac tccgatttac ctcaacccag ggctggggag
     7201 aatgaatgat catcatatcc agatagtcca gtcccatttt gctcagtgtc tcatcaatac
     7261 tctttgcagc agattcgtag gtcttgtgct ctgctgcaac ttttgataca acaaaaagtt
     7321 cctctctggg tacaccgcag gtacggatac cttccccgat accgcgctca ttgccataag
     7381 cctgggcggt atcaatatgt ctgtaaccaa gttttaccgc tgccttcacg gcatctgccg
     7441 ccttgtcatc atcaatgaac catgttccca gtcccagctg tggaatcttc acaccattat
     7501 ttaaagttaa ttcattctga tacatttctt ctgctccttc cataatcttt ctatttgacg
     7561 atcttgctca ttctgcccga aatcttgcat atgtcctgtc tgcataataa tgtttcattt
     7621 cggacaattt tatcataata cctggagcta actttaggtc aatacttttt tgctacgtcg
     7681 gtaatatttg ttcctgcata gtcataaatt tttaattgca tcacagaaaa tggtatgtta
     7741 tactatgaaa aataaatcga aacaaaaatc aggtagcaat gtcagcgtgg acatcttcgg
     7801 atgcccacgc tttttctttt gcctgataag acaaaggagg agaatgttat ggtagagttc
     7861 attacgaatg tgaacagtgc cgtcaatgga ttcgtatggg gtgtccccat gctggtactg
     7921 ctggtaggta ccggtattct gatgacgatc ctgacgaaat ttttccagtt atcccacatc
     7981 ggacactggt tcaaaaatac agtaggcggc atctttcacg acaaacacgt aacaaagcac
     8041 acggatgctg atgatcagtc catctcccag ttccagtcgc tgtgcacggc gttagctgcc
     8101 accatcggta ccggtaatat cgtcggcgtg gcaagtgctc tggtagccgg tggtcccggc
     8161 gccatcttct ggatgtggat cgtggctttc tttggcatga tgaccaacta ctcggaaaat
     8221 gtgctgggta tctactaccg ccgccggaac tccaaaggtg agtggagcgg cggtgccatg
     8281 tactacttaa gggacggtct cggctcctat aagggctgca aacagattgg tgccgtactg
     8341 gctgtgctct tctctgcctt ttgtctgtta gcctcctttg gtatcggcaa catgagtcag
     8401 gtcaattcca tcgctgccaa catgacctcc gccttttcca ttcccgctac cgtcaccggt
     8461 atcgttctgg ttatcatcgg tgcgctggtc attgtcggcg gattaaaaag aatcgcagct
     8521 gtcactgaga agctggttcc tttcatggca attgcttatg tcatcggtgc actggtgatc
     8581 ttttttgcaa atatcaatca catgggggcc gtattcaccg ctattttcaa aggtgctttc
     8641 ggtctccgcg ccgtgggcgg cggtatcgtg ggctccggag tgaagctggc tctgacctgg
     8701 ggcatgaagc gtggtgtgtt ctctaacgaa gccggtcttg gttcctctgt catggtacat
     8761 tccagttcca atgtaaagga gcccgtgcgt cagggtatgt gggggatctt cgaagtattt
     8821 gccgacacca tggtcgtatg tacgctgaca gctttcgcgg ttctgtcctc cggactgatc
     8881 gacctggata ccggtgccgt actggtggat gtgtccggtt ccgctctggt gggtcaggct
     8941 tttgcaaccg tattcggcag cttcggtccc gccttcatcg ccattgcgat cctgctgttc
     9001 gccttctcta ctgtactggg ctggagtcac tacggatcca aggcatgtga atatttattt
     9061 ggtacgaaat ccaccattgt ctacaagatt gcattcgtac tgatgatctt catcggctgc
     9121 accatgaact taggtcttgc ctgggatctg tccgatacct tcaacggtct gatggcgatt
     9181 cccaacctga ttggtgtcat agccctgtct cccgtagtaa tgaagatcac gagaaattac
     9241 gtggcacgta tcattaaaaa agaagatgta aatcccatgt tatctgtctt ccccgaaatt
     9301 cagggagagc aggaaaaggc catgtaattt aacttagaat catacttctt ctctggtaat
     9361 attcttcgcc catctgccct ctcggtaatg cttaatgacc cagggccatt tgacgaattc
     9421 atccgtgcat aacaggaaat agacccacaa caccgggagt ttcagtacaa atgcggcgaa
     9481 gaatcccaga ggaatcgcat agcaccacat atcgatcgta tcgcagatca gtccgaactt
     9541 cgtatctcca ccggcacgga atactccggc gatcagcgcc gtattcacgg cagcgcccat
     9601 aatataataa gtattgatga gcagcatata tttcagataa tgcatggcag tttcattcaa
     9661 tgtagcaaac cgtaacacaa agggtgtaat tgccagtacg atcaggccgc ctatagctcc
     9721 ggcgattacc gtcatcttca acatccggga agcgtaactc ttcgacttct ccagattgcc
     9781 ttgtcccata atatttccca gcaatatgct acctgcactg gcaatggcaa agcagaacac
     9841 cgtaccgata ttgcgcacta cgacgaccag tgaattagca gccaccgcat ctgttcccag
     9901 atgcccaagg atcacggaat acatggaaaa cgccacactc caggacacat cgttgcaaag
     9961 tgcgggcaga gacagacgca tgaaatcttg tgtcagcacc ttactgtgaa caaacaggta
    10021 cttcggatgc agttttacgt ttttgctcaa gaaagataca atgaagcatg ccaccagttc
    10081 caccagtcgg gacagggctg tggcaatggc gacaccggta gctcccagct ttggcatgcc
    10141 gaacagtccg aagataaatg tggcattcag aatgacattc aatacgaaag ccagcatatt
    10201 caacgccata ctgaccgtca cacgacctac gcttcgcaga atggctagat atacttccgt
    10261 gatgccccag cacaggtatg tgatgcccat gatccgcaga taagaggcgc cgatagtgat
    10321 cagctcctga tcaggagtaa agagaagcat catcttctga gggaggaaaa atgcaatcag
    10381 cgcaactata cctgaaattg ccatagagaa acgaagtgtg attccttcta cagtatgaat
    10441 tgcttccagg ttcccctttc cataatattg ggcgcacaag agagtggctc ccgtccccag
    10501 tccgtaatat accataaaca ggacattcga aaaatttgac gccagggata ctgcggatat
    10561 tgcggactgt cccacatagt ttagcataat aacatccgcg gaattcactg ctgcactgag
    10621 cagattctgt ataatgatcg ggatggtcag tttgaacatc tgtctgtaaa aatcgtcttt
    10681 tttcattgct gttatctgca tggtattcct ctgtgttcct ctccattgga taattttctg
    10741 ccgttggatt gtattatatg tcgttcgcag gaattacgca atatgctaaa agcgatccgc
    10801 ctgtaaacaa gcggaccgct ccttttaaaa aggctgtttg attataatgt ccagccggca
    10861 gcctccatct gagtggcttt catatgccgg taaatgccgt cttttgcttc cagttcggca
    10921 ggcgttccct gctctgccac cacgccatcc ttcaatacaa caatcttgtc tgcgtccgcc
    10981 acggtacgca tgcggtgcgc aatgatcata acggtcttat tgcggatcag cctggaaagg
    11041 gattcctgaa tcagtgtctc gttatctaca tccagagaag cggttgcctc atccatgagg
    11101 atgatgggag catcctttaa aaaggctctg gcaatggaga tccgctgtct ctcaccgccg
    11161 gacagttccg aaccgttctc accgatcatg gtctgccagc cgttcggaag cttctccgcg
    11221 aactcatcac aatgcgccag ttttgccgca gccagcactt cttcatccgt agcatcctgc
    11281 ttgccgatac ggatgttttc catcaccgta ttattgaaca gcgtcacatc ctggaatacg
    11341 atagcataca gagacatcaa ggtctccgga tcaatggtgg agatgtccat gccgcccacg
    11401 gtgatcttac ctttattgat atcccagaac cgggaagcca gtctggacac ggtggtctta
    11461 ccaccgccgg aaggtccgat caatgcggtc acttcgccct gttttgccgt gaaagtgaca
    11521 tcgcgcagta cggtattctg actgtcatag gagaatccca cattggaaaa tgcaatgtca
    11581 taccccttag gatccatggt gtcggcaccg gtctggatct catgggacag gatctcgtcg
    11641 agacggtcac actgcacact ggtactgata atagctgcca gattctgcag tgccacctgc
    11701 aggggatcgt acattctgga taccaatagg aggaatacga agaaggtcag gatgtcgatc
    11761 tctcccttta ctaacaacac accgccggtc aatgccacgg tagcgatgcc aagctttaag
    11821 atcatctggg caccgcctac aaagactgcc gtaccaagct ccgtcaccag tgcatgtttt
    11881 tccacggcac ggatcttgcc ctctaagcct tccatgtact cagcctgggc attgttggcc
    11941 cggaggtcac gtacggtctc caggcattcc tgaatgccgt ccgcgcagtc catttttaat
    12001 ttcatctgct tcttacttaa gctcttctgt accctgccgg aacatcccac aatgaggaaa
    12061 gacaccggca gtacccacag tgctgcgatt gccattctcc agttcaagaa aaaagtaccc
    12121 acagcgatca gtgtggtgga gatacaggaa ccaacaagtt ccggaatgaa atgggaggat
    12181 gcggtctcca tggtggcgca gtctgccata atggtgctgg tcagatcaga cagatctttt
    12241 ttcccgaaga aggataacgg gatcttacgc agctgctctg ccagagcaat tctgcgcacg
    12301 ccgctctcca cataagttgc caggaacgtg gcacgatact gcaccaacgt tgtcagcgcg
    12361 atcagaagaa gacagagtac gcagcccaca atgtagaaaa cacctctgcc tgccatggtg
    12421 ccctgtaaat aatctcttac cagaaaatac agcatccata ccgggaacat caacaccaga
    12481 tcggaacatg ttacggatac aaatgccttt atcatatcct tcgcaccttt ttcggaaagc
    12541 gcgtatttat gctgcaattt tgcctgtaag ctactcattc tatttctccc cctttcctac
    12601 tttccagctg acggatttat tgtactcttc ccacatatgg gcatacagtc cgttttgttc
    12661 cagcaatatt tcatggtttc cctgttccac aattccgcca tccttcaaca cacagatgcg
    12721 gtcggcatcc acaaccgagg acaatctgtg tgcgatcatg atcacggtct tgcctgcgga
    12781 aagcttcgta aatgcctgcc gtaccttcgc ctcattatcc ggatcagcaa aagcggtagc
    12841 ttcatccagg atcaggatcg gtgcattttt taacatcgct ctcgcaatgg acaggcgctg
    12901 tgcctcacct ccggatacat aagtaccctg gcttccgatg acggtatcca caccgtccgg
    12961 cagctttgca ataatgtcct cacactgggc atttttcaga gcttccatga cttcttctct
    13021 ggtggcatcc tttttgccca tgcggacatt atcgtaaatg gacatcttaa gcaatttgct
    13081 gtcctgaaat acgaaggaca ccatattcat caactgtgcg gaaggaatat ccttcacatc
    13141 cacgccgccg atgcatacct taccttctgt ggtatcatag aaacgggcaa tgagacttgc
    13201 cagcgtggtc ttaccgccgc cggagggacc gacaaatgcc acatgctctc cttctttgat
    13261 atcgagattg atctgatgca gagcatctct tcccgcattt tcatagcgga aggatacatt
    13321 ttgtaacgca atggaatgat ccttgatgtt ctgctctttt tctgcttccg gcagaggatt
    13381 acggcttaag ataccatcag ttcttttcag agcatcctcc acgatcatct gattctctcc
    13441 gccgtacatg atcttgccta atgtaacggt cagtaccgga gtgataatga tgtagaacag
    13501 aaggttgatc agcacctcag gatccccgcc tgctccaccg aaccaatatg cggcaccgat
    13561 cagaacggcg aagatcgcat tgatcagtgt ggtaaagcct accatgggcc acatcagttc
    13621 tttggtataa gcgatggtcc atttttcata ttttttaata gaggtctgga aacgcttgaa
    13681 agaaaatacc gactgtccga aggtcttcac taccggaata ccgcggacat attccactgc
    13741 ttcactggac atctcctcta acgcattctg gtattctgtc attttatctt tcatgcgctg
    13801 tccggtcatg gcacccatga tgaggaatgc aaagacgacg ggcacgagac ttaacagccc
    13861 cagtttccag tcaaatatca ccaaaagcag tagcagtccc acaggagttg ccatcgctcc
    13921 cgccttatcc ggcaactggt gtgccagata ggtctcagtg gctgcggtgg attcgttgac
    13981 gatcttacgg atcttaccgc tgccctcact gtccatgaat cccatgggca gcgtcagaat
    14041 atgacgcata agggtgctcc gcatattcgc ctgtacgtga aaggctgcaa tgtgggagca
    14101 caacagtgcg cagatataaa tgatcatgga aagcagtgca aatcccacgg cactccatcc
    14161 aagccccgac agattctgcg ccttgctgta atccggcgct gccagtacaa cctcctgcat
    14221 gatccgccag atatcataaa acggcagtaa tgcaatcagt gcactgaccg cggacagaat
    14281 ccatgacgca atggtcaggt acttgtagtt gcccgcataa gcgaacagct tctgcataga
    14341 gctttctttt ttcatgtgat ctttccctct ttccctttga tttaaattaa ttttttggat
    14401 ttaatgtaac tctttaattt ctagaatttc ttttcatttt gccctgcttt tcatcatttc
    14461 tcttttccgg acatttgagg ctctattgtc tttcctatct aacaccgctt attgataatt
    14521 agtttcaact aaccgtcggt ttttaagata gactgctacc gtaatcttcc atagcagtct
    14581 tatcttatgc ttcaccccag cttgatgatg gctccccatc cgccgtagtg atacttctcc
    14641 agcatatgca gatattttct ggcagtatcc ctgtccatgc ggtgtctgac gacctcgaac
    14701 atactgtcaa acatggctct cgacatgata tgtaggaact cctcggtgat catgctgtct
    14761 tcctgtagga attgtgtggc ttccatatac ttgtaggtat actccgtctc aatgtccacc
    14821 agatgttcca cgaaatcctg atacctggta ccgtaagagg catccagcag taaccgaaaa
    14881 tcatcgaacc gatcatagac ataatccagc atttcatcca tgccgtccat cacgtaggtt
    14941 tccatcacct tcggctgttc ctctgcctcc ctggcatgaa acgcctcctg ggtcttaagg
    15001 aatatctgcg tcagcccttc cgccgccggt tccacaatcg cacaaaacaa tccttccttg
    15061 tccccgaacc gggaatagat ggatccggta gtagtgtctg ccttggctgc gatcgtacga
    15121 agcgacgctt cggaatagcc cttctccagg aattcttctc tggcacagac gagaattctc
    15181 tctgaaactc cttcgattgg ttttgtcact ttttcctcca aaaaaatatt cataacagcg
    15241 ttatataaca ctgttatgaa tataaacaca tttcattttt ttgtcaagag ggatcatagt
    15301 tttttctctg ctaaagctgt cagctcctgt aatgtacatt cagccagttt tgcctttaca
    15361 ccggcgatat ttccactgct catagacagc tttctcgctc ccagtcccac cagcaattgg
    15421 gcggtctccg gatttcctgc catttctccg cagacagata tctccttttc ccgtttggaa
    15481 aactcccgga atacaaaccc aagtagccga agcattgccg tagaatcgct tttgtaataa
    15541 gcggtcacct ccggattcat ccgatccgca gccgacaaat actgcgtcag gtcattggat
    15601 cctatgcttg caaaatccac ctcttccgcc gccagatctg caatcaaggc aatcgcggga
    15661 atctctatca taataccgat tcggatctgt tcatcaaact gctgctttct ctctttcagc
    15721 tgctcttttg ccagattcag gtactctttt gccttgcgaa tatcttccag actcccgacc
    15781 atcggaaaca tgatttgcag aggtccgtaa gcagaagcac gcagtgccgc acgcagttgc
    15841 gttaaaaata actctttttc cgcaaaacaa agcctgattg ctcttttccc caaaaaagga
    15901 ttgtcctctt ttggcaattc catgtagggc aatgtcttat ctccgccgat atccagtgtc
    15961 cgcaaagtaa tcggatgtcc ttttgccttt tgcacaacct gcctataggc ttcaaattgt
    16021 gtttcttccc ccggaagttc cttcccttcc atataaagaa attctgtacg gaacaagccg
    16081 atgaaatcgt accggtctgt cggaacatca aattcctgcg agccgatatt gattcctatc
    16141 tttatttctg ttccgtcctt cataattgca ggtttgttta aaaaattttt ttctgcttct
    16201 tttttgcgga aaaaagatgt ctgtttttcc ctgatatcgc aaatacattc ttctcccggt
    16261 gtgacaaata tttctccggt caaaccatcc atggcaagca tagtatctgt aggaattttt
    16321 tccgtaacat tcggaactcc cagaattgca ggaattccgt aacttcttgc caggattgcg
    16381 gaatgtgaat tcactccgcc aacctccgtc aggattcctt tgatatgttt tctgtccatg
    16441 gtggccgtat cactgggtaa taggtcatgg gctaccagaa ttacatcttc cgaaaacgaa
    16501 gacagcttag acgtttcctg ccccgacaga atccggagca gccggtctct gacatctcta
    16561 agatccgccg cgcgctcggc aatcaacgga tcctccacac cggccaacaa ttctgcaaat
    16621 tctgtataca ccttactgac cgcgctgtcc ggattcatgt tttcctcaca gattgtctcc
    16681 cggatctcat caaggatttc ttcatcctga agtaattccc gatgtgcact gaagattttt
    16741 gccttatccg gatcctcttt acacataccg ttgtacaatg tcaccagttc actgtctgcc
    16801 ttttcacagg attccctgaa ttcccgatat ttttcctctt cctgaccctt gggaaaaaaa
    16861 tccggagcct ttgtcagtgt accgggaatg tagatataag atttcgcata agcaattccc
    16921 tcagataccg gatttccctg taatctttct tccatacaca tttaccgcct ttattctcca
    16981 aatccgcttt ccaccaaggc tgcaagagac gctgccgcct gctcttcatc tgtgccttcc
    17041 gcagcaatct ctattttggt tccctgtccg attccttccg ccagaatccg tacaacggat
    17101 tttgcattca ccggtactcc gttttccctg caaatcgtaa tcttgctttc atattttttg
    17161 gcttccagta caaaatcaga agccggtctg gcatgcagtc ctgtcttatt cacaatctca
    17221 acctgttttt ttgtcataat ttatcctctt ttttattcgt ggaaagtacg ctggctccgt
    17281 tcgtcctgat gcactcccga taccaggcaa aggaatcttt tttctttcgt tccaaggttc
    17341 cttttccttc atcgtctttg tccacataga taaagccata gcgttttttc atttcaccgg
    17401 tgcttgcact gatcaaatcg atacatcccc acggagtgta tcccagtaac tccacaccat
    17461 catttactgc ttcccacatc tgtctgatat gttcgcttaa ataagcaatc cggtaatcat
    17521 catgaatctg tccgtcttct gtcacacgat ccaccgctcc cagtccgttc tctacgacca
    17581 tcagcggaat ctgatatctg ccgtacagtt cattcaggag gtaacgtaat cccttaggat
    17641 caatgatcca tccccattcg cttgcttcca gatagggatt cggcacacca agcatcatat
    17701 tccccgccgt ctgtatcact ttcggatctg ccgtggcaca acgggaactg taataactga
    17761 agcttaagaa atccactttt ccattcttca atatttcatc atccccggcc tcttttcgga
    17821 tctgtacccc attttcccta aaaaagcgtt ccgcaaaata aggatatgct cctcttacgc
    17881 acacatctcc gcacaaccag ttgctgcgtt ccatctgtgc ctgtgcttcc actacatctt
    17941 ccggactaca agtatatgga tatacaccgg ccgcagacag catacatccc actttatttt
    18001 cgggattgat ctcgtgtgcc atttttactg cctgtgcacc ggccagaaac tgatgatgca
    18061 atgcagtaaa tctgcaactc ttttctgccg cggtctcggg ggatcccagt cgttttctcc
    18121 cgaacaagtc ttctccgtcc tcgggtaaaa tgccccctgc aaggagcgtt ccgaaacggc
    18181 tgaccagtgt attcatttca ttaaatgtca gccagtaatg aaccagatcc tgatactctc
    18241 tgaataccgt ctcacaatac cgcaggaaaa atgcaatcgt ctgtcggttg gtccagccgc
    18301 cgtaatgttc gcacagataa taaggcattt cataatgaga caaggtaact aacggttcta
    18361 ttccgtattt tttacaggtt tcaaatactt tcctgtaata cgcaatcccg gcacgattgg
    18421 gcgatatctc atcacctttg ggaaaaatac gggtccatgc aatcgaaatc cggaatactt
    18481 taaatcccat ttccgcaaat agtgcaatgt cctcctcata tctgtgatag aaatctatag
    18541 cttcgtgact tggatagttc tcctcagggc ggatctgttc cgtgaatcgc cgcggggacg
    18601 tttttgcccc cgcagtaatg tgatccgcta cagaatctcc tttcccgtcc gcctgccagg
    18661 caccttctgc ctgattggct gccacggcac cgccccatag aaatccgggt ggaaatgttt
    18721 tctgcttttc ttccattatt tttcttcctt tgcctgtgct tcttttgcta ccagttcctt
    18781 ttcatatact ttgaagaacg gccaataaca tgcaaacgcc actgcaaaag caatcaccgg
    18841 aataaagata ttagtccagg acaaagtcgt cagatactgt gcaaatccaa ccggaaggtt
    18901 cgccgtaatg gaaatgtaag ccggtttgaa aaatcctatc atatatccaa aataacagat
    18961 gatcatggta attacagggt tcaggataaa gggaatgcac atgatcggat tatacataat
    19021 aggataaccg aagatcacag gctcgttaat attaaacaga ccgggcacga ttgctgcctt
    19081 gctgactgct ttcagctgtt cggatttcga gaataatccc atcacgcaga gcgccagtgt
    19141 attgccggtt ccgccacagc aattgataat accgaataac gcgattggca taaatacaag
    19201 cgcctgaccg gcctcatgat tggcatagtt ggttgcataa tactgcatta acggtgctgc
    19261 gattgccgca tataccgcca ctgttccatg tacacccatt gtccataaca gactggcaat
    19321 gattacgata ataataattc cgggaacact tgtcagtgct gccagcggca ttcccagcag
    19381 accggtaata agtaccggaa gcgctgtctg tgtcaatgcc acacagccat aacccaatac
    19441 cgtccagata atcgcattta ctaccagagg gatcagagaa cggaaggtat cctgcagaaa
    19501 ttccggaacc gcatccggca tcttgataac cagatgatgc ttttcaaaaa aatgtgtcaa
    19561 ttgtacgctg gtcagcgcta caataatggc tacaaacata cccttcgcat caagcgccat
    19621 ggtatccatt ccggtaaaag ttccgcccgc ttccccggcc agatctactg ttttgatcgg
    19681 cgcggctgca atcaaaaaga tcagaatcga attcagtcct gccatcagcg cctgcatttt
    19741 taaagacttt gcataagaat aggcaatggt aaaagctaca tacagcgaaa taaatcccat
    19801 agacacctta tacggcacca taagtgcact atagattccg gaactctgat cgataatacc
    19861 gatcgtctgt aatatgactg ccagtaattg gaataccgct cccaacaata ttacacccat
    19921 taccatcatc ataccggagg aaatcgcctg tattccctta tttcccacta ctttttcccc
    19981 tgcttttttc acccattgca taggcttgct ttcataaaaa tttgccatac ctttactcct
    20041 tccctgttga aataccttct ttttacgttt tgcagactta ttgatacaag tcttccacct
    20101 gcttcataat attggccaca ttaccgattg cataatcata tgccggaatc accgcaatag
    20161 gtttttgcgt attttgctcg atctcaccct ttttataaga tacctgcggt cccaacagaa
    20221 tgacatcata atttttgtac acatcttcat atgctgacat tcccactgct tcgatcttca
    20281 gagaaattcc ctgttcctct gcatattttt ccatcttacg catcagaatt ccggtagaca
    20341 tcccacctgc gcagatcaat aatacttttc gttccatacg attcctcctt gcttttccct
    20401 acttttgata taaaacgatc atttcccgga tcaaatccag tgccagtgca cttcccatca
    20461 gatgatcctg cgcatgacac aataaaatac ttacctgcgt cacttctccg gacgcatcca
    20521 ttttcaacag ttctctgtga gattcatgtg ctgtttgaag actctcttct gcctccttca
    20581 gaagtttctc tgcgtcttcg aaatttcctt ttttcgcctc ctgcagtgca gcgaaggcat
    20641 ctgatcttgc ctgtccggaa ttggctatga tcatcatcgc gatttcttcc agcttttcct
    20701 gcatattttc ttccattttg taacctcctg ttcttagagc tgatatatga tcgtcagaat
    20761 atttgctatc tcatcatcat tgataatgac atgaaatgtc ttttccaacg gttttactat
    20821 tttaagcagt tttttaaatt ccttgtcata ttgaagcaat attgctgctt ttctgggatt
    20881 tgtggcaact tcctgttttg caagcagcct gtcaatacaa caggaaatat gaatcaacaa
    20941 gccggcttcc gcatccaaag ataactccct aagcgccgca ttgatttctt tcataatatc
    21001 cggcagtatt tttctcagct tttcaatatc ggtgtgtgtc agctgttctc ccagatatgc
    21061 aaacattgca tcataatcta tctttgcttt tgcctctttt tccagtcgga gcaatcttgg
    21121 aagactctct tttctggctc cgaatacttc actgatcggc aggaagggaa ttgcaaacat
    21181 tcccggatca taggttccca caatgcactg aaccactccc gtggtcatca ggtgtttcag
    21241 gttatcacgc aacagttctt tgtctgtaac cgacatcggg atcacctctg tatcttccaa
    21301 ctgtccatat ttttcgatgt aacgtttcag ttcctctgcc ccgcctttcc cggtggtaca
    21361 gagcgttaca atgtattttc tgtaacagcc cagaccatct atactctcca ctgtgctttg
    21421 caatatcttg tcgggatcat tttctgtaag ggtccgcctt gcaagctcaa ttccaagtgt
    21481 agttaccgga actctgacct gccggattgg aatttccagt tcattttcta tctccagaag
    21541 catttctccc aaaaaactac tatcatagat gaccagaatt cccttgccct gctgcacttt
    21601 tttcaggaaa tttctcagtg tcccatatac ctctgcggag gctctctcac tggtaatttc
    21661 ataagaaaaa atattatcca gctttgtcat gttactgacc gtctgtgcta tggaggaagc
    21721 tattcccaca ccgtagaatg caaatatcaa taccggtctt gcattttccg tcttttcctc
    21781 attttgatag cacaaaaaca ttgtgagaag taagatttct tccatggaca attccactgc
    21841 gtattctgct ttgatctgtt ctgcaagttc ttcgcttagc agatattctg ctctgtgata
    21901 taccagtatt tctgcaatac tttctttgtc cggagcactt ttttctcttt ttcccgtaat
    21961 cactgcattg agatgaaggc acagcccata tagtacagac ggagagaaat tacgtttcag
    22021 ctgttccttt gccttttgca aaaatgcttc cacaatattg atcagctttt cttccaccag
    22081 gatttccaac tgttttttat ccgtcacatc ctgtatcagg gctttccgat atttatcaaa
    22141 agtcctttct acctccatgc tcagcaaaag gttgatctct tcctcctcta ttcctgtctc
    22201 ctttaatgct gctgccttcc ggctgattct gtcatatagt gttccgtctt ccggcctgct
    22261 gacccggatt tcttttccgc tgaacgtgaa tctctgttca aaatcgatca actcctctgc
    22321 ttctctccgg tactttaaca tgcctttgcg cacattattg gaaaaatcgc tgataaataa
    22381 tgaaatatct cctgttttcc catattctct cacataggca ttggcgcagc cgatcttgat
    22441 atctcctttg agctgataat aatttgcttc acactcatag aaaagcaggc atgtcatcaa
    22501 atctcctctg acgatcagcg gtctctgtat ccgcacagcc tctttggaaa aaaggtgttt
    22561 tatcatttcc atccgttccc tcagcggccg ctccgacaat gtcggtaact gtaactgtac
    22621 tgaaaattct gccaggaact catccgacag ctgttctttg tctgtacagc ttaccgccac
    22681 cgcatatttt ctggatggtg actgcagata ctccagcacc ctttttctgg ctctcgggct
    22741 taaaaactga atattatcga aaaatacaaa gccctgctcc gactgtttcc agctgtcatc
    22801 cgactcagca ctgacttctc cgcctgctgc ataatcgtgg caatcgatgt gaagaaacgg
    22861 agcattttcc ggcagaatct tctgttccac cgcaaaacgg tacattcttt gtgccaagcg
    22921 gctcttaccg gttccacggg ctcccaccaa taacgtattg agacttcgct gtggatagag
    22981 tactgctgcc tttgccagct gaatggtctg cctcagactt ccgtccgctc cgatcaaatc
    23041 agaaaagcat tcttccaatg tgctttcttc tcttccaacc ttatagagaa ccggtctgcc
    23101 gttacatttt tgtatcctgc cttcctgtac cagaagattc agtatgctgc ttacattcgt
    23161 tcgctgtagt tccatggcat ccgctatgta tgcggtggat attcctctgt ctgccttttc
    23221 atctgcatga agtctgataa agtcatatac cttttctttg tttgatttca tagggtgcat
    23281 ctccttttca acgtttgcat ctatgtgtat atccaaagaa aattatacac tctatacaat
    23341 atgcaatcta tcattttccg aatatttcgt caaaagaagt tgtgtatctg tttttgtcat
    23401 aaaaaatata tttattcatt gattttgatg atttttatta tgattttttg gtactttttt
    23461 acagaaccat aaaaagaatc cggatgaagg atcattcgga ttctttttag tgtatatgat
    23521 tttattttgt ttttgagaag tgtataagtt ttttgttatg gtgttgtgaa ttctatcggc
    23581 tgcgcatctt ttccatacgc atcccatgcc tgcagatact cctcctcggt gctttccaca
    23641 cgctgtcccc ggctgtccag acgataatag gtttcattgc cgttctcgtc aaagctttct
    23701 gtattggaac cggtcagtac cagctgtccg gtcgctgtat caaggttgta cacttcgatg
    23761 ctgtgtccgg aggtcacgta gaacccagac attccgacgc agatcggata tgcggttccc
    23821 tcacttagga gctcgccgat ctgcaccggt gttccatctg ctccgctgca gtagacgtaa
    23881 cagtacatgg cataggtatg ctctccgtcc tgataaatcc cgtccgacaa taacaacaga
    23941 gtcgtatcac caagctttac ctgcgcatac gctgcatctc cctttcccgt tacaaaaccg
    24001 gcggttgcac cgtcctgcat attcatccat gtcgtagtat taccattctg cattggaatc
    24061 cagtcggcag aattttccgc tgcctgtttc tcctgctctg ccgcacttgc ttccttcacc
    24121 gcctctgcct gttctctctg tttatccagt gcctcctgca atgcggcgtt cgtctcctgt
    24181 tgtgcattga cttcgtctgc gcttgcgaca ttcccggcat ccacaccgtt ttcgctcttt
    24241 ccggcctcat tcgtcagcag gattgtggtt gtcgtcccct gctttggatt cgtcagaaaa
    24301 cataccgcta cgatcacaca gaccaccaca gcggcaagca ccacccaaaa ggtcggcttc
    24361 ttataattca atatctcttt cactctcttt ttcacccctg cttccccaaa ggccaacggg
    24421 cataccgtca ccagccgcct ttgcatacta caggaaagca gtgcatagga atagtctttt
    24481 cgctcctgag gattcagctt cttcaccgcg ctctcatcgc atgccagttc cagatcgctg
    24541 cagaacagga tgtacgctgc ccagacaaaa ggattgaacc aatgaatcgc taagatcaga
    24601 aatccaagcg gcttccacca ctggtcgccc cgtttcagat gcgcctgctc atgagcgatg
    24661 acgtaagggt actgcgcttc atccatactg gaggacagat agatcttcgg cttatgcagt
    24721 cccaggataa aaggagattt tacctcatca cagatccaca ggttctcccg cagccacata
    24781 gcttccgcta cctgcctccg caccctgacg taactgtata ccgcatagcc tgcaaaaaga
    24841 atcacacctg ccagccagag aatcgagaat gcagataata gccgttccgg tattctccat
    24901 actcctgctc caaatgctcc gatgccactt ccggtaattc cgaaagctcc gcccatgccg
    24961 gatccggctg cgcccatgct gccgtctaaa gctgcctggg aaatattgtc ggaaaaatta
    25021 gttccgtttt gcagcgtatc agcccggaac gcattccctg ccggactgcc tatcatattt
    25081 ccgtacaaat agcccgacaa tatcggcagc ttcggcatca gactgaaccg gctctccggc
    25141 acaaaagggc acaccagccg cagtcccaca agcccccaca gcacaagatt cacccacttc
    25201 ggtgcccgtt tcagcaagcg tctgagcacc attacgacag ggatcatcca tgccgcggca
    25261 atactcatct gtaatacacc cgaaaatatg cggctcattc ttttccccct tctgcctcat
    25321 cgatcatcct acggatcttc gcagcttctt ctgcggtcag cgcacgatct ttcgtaaatg
    25381 cggcaaggaa cgcaggcaat gacccctcaa aggtgtcttc cacaaactgt ctgctctgca
    25441 tcgcatagaa ctcatccctg gagatcaggg aagtcaccgt tccgttgtca ttgcgaaaca
    25501 gccccttatc gcacagtctg cgcagcacat tatgcgccgt ggtgcgcttc cagtgaaatg
    25561 cttcctccgt cagcttcacc agctccgaag aagacaccgg ttcgtgctcc cagatgatat
    25621 ccgcataccg cgcctcgacc acacctaact gaatctctgc catgttgttt cctcctacta
    25681 ctgttttcct gatgacatca attttgatat ttcacgttta cttttgcaaa agtatagaga
    25741 ccacatgttg tggtctaact acagactaca acatgtggtc tctattgtca agtatctttt
    25801 ctattgcaaa tgatatgtct gtggccatcc tgcccgtttt ccgcactcga tctcccggcc
    25861 gatcctttca aagatatatt ggtttttgtg ctccagatcg atcagctgca gcatttcctc
    25921 atccgtcagc tcacgctccg gctcatagaa cctcgcatca ctgatgcaat aggcaagctc
    25981 ctgtccatcg tattcttccg gatcagttat gaccttaatg ggtgactctg tataggcatt
    26041 ttccacttca tacttttccg tccattgctc atagcggctc tgttcctctg ctgtcaggtt
    26101 acgactgtct gttccccata catctactgg tgcagaagcc agtaaggcat ataattcatc
    26161 aatctccgca tccgtcatct gttcatatct gtcaaaatag gaaacctcgg aatacttctg
    26221 ctgccatagt ttttcccgaa ggctgtagtc cagtttatgc agatactcta taatctccag
    26281 ccactcttcc tctgtgagca cagtatccgg cagatagatc agacaaccgt attcttctcc
    26341 gggagtactt cggactgcca caccagtgcc ggaatagttc tcgtcttcgg ctagttcccg
    26401 gatctgtccc gccggatatt tgccgtccgc ttcgtattct cttgtcagct gctcataaat
    26461 gctccgttct ctggctgtca actctctact gaattctgct gtgacattgc cctgctccgc
    26521 taccatctcc aaatgcccca actcctctgt accaatgtct gtcatgcgtt ccgccatgta
    26581 cctacggtaa ccaacgattc cgacggcagc ggcagagaca gctgtggcgg aaagtattac
    26641 taccgccaac aggatgcctg ctgcccgggg aaagcttctg actctcctgc ccgcaggact
    26701 ctcctgcgct gcctgttgtc tgatctgtgc ataggctgcc tccgccctat ccagtacgat
    26761 ctgtggaagt tctatctcta tttcattcat cggtctcatt ttcttccgcc tccttcctct
    26821 gtctccgcat aatatctcgc caactgctcc cgccctctgg cgagccttgt gcgcaccgta
    26881 ctgtcgggca gctgcagtaa ttttccgatc tctgctgtcc gcagtccttc cgcatagaac
    26941 agtactacga tcaggcgata tttttcatcc agtccccaca gggcttcctt ccattccaaa
    27001 tttgcctctg cctccactgt atccttcgct gcctgttccg gcagttcttc cacacagatc
    27061 atccgtctcc ctgagcgcag gatatccttg catttattga tcagaatacg gatcatccag
    27121 gtcttgaaat atcggttttt ctgtaatgta tctattttct cccaaacggt gagaatcgtc
    27181 tcctgtatgg catccgcaat atccgcatcc tgcggtagga ttgcgctggc tgtccggtac
    27241 atgtccttct catgaagctg catcagctcc gtgaaagcat ctggatcccg tttttttgcc
    27301 ctgcgcacca attgttctat ctctcccatg tgcctcgtcc tttcttcctc ataagtatac
    27361 gtcttatgac tattagacgg aagagtctgt gaaaatgtcc cggggataaa taccaaaatc
    27421 aaatcaaatt cctactttag aaaaaacttt tcagcctgtt acgagtcaaa gctctttttt
    27481 tgctacatat agtccgtata attttctttt tattcctctg actactaaat atggtattgt
    27541 gcactttttt gttgtggaat tatatacgaa atattagaat tgaaacgcct tcacacgcat
    27601 gatacacttc ttgagaagca accgtgttac aaggtggtag cctcccggct tatagctttt
    27661 aatgagaagg gaggtggtgc taatgaatat gtatgaagta ttaaacctgt tatttttagg
    27721 tggtacattt ctcattgcgc tgcttgccta catagacagt aggataaaca agcgaaaata
    27781 aataaaccta ccttgtcact tggccagtac aggtaggttt actaaacaat taccgggagg
    27841 ctaaccactt gtgggcggtt gcttctcttt atgtcttgat tataatatct ttctttcttt
    27901 ctttcaagcc ttagttttaa aaagttcggg taaatttact gtaggaaatt aatagacaga
    27961 gcatccttga gatatgtt
//
LOCUS       DAWDHD010000015        27470 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_142_length_27470_cov_14.038410, whole genome shotgun sequence.
ACCESSION   DAWDHD010000015 DAWDHD010000000
VERSION     DAWDHD010000015.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 27470)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 27470)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..27470
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142_length_27470_cov_14.038410"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1912..2011
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         8238 a         5451 c         7038 g         6643 t
ORIGIN      
        1 tgttgacgat aagccatttc ttgtatataa taaatcgtat cactgtatac aattaagaaa
       61 atgtacaaga agaaaaattt gaggaggata ttatgacagg aaatacgctt ggatgggtaa
      121 ttgtggcatt cgtgctgtac ttactcatga tgatcgctat cggagcgttc tatgcaaagg
      181 gcaataacaa ttccgaagat tactttttag gaggcagaaa gctgaacggc ttcgtcgctg
      241 cactgtccgc gcaggcatcg gacatgagcg gatggctgtt gatgggactg ccgggagcta
      301 tttatttaac cggtgtcggc ggtgacggct ggatcgccat cggacttttc atcggcacga
      361 cgctgaactg gctgttcatc gccacgaagc tgcgcagata tacgatccgt gctaataatt
      421 ccgtgacgct gccgacttat tttgagaacc gcttccatga tgataagaaa gtattgatga
      481 ctgtttcttc tattactatt gtagtatttt tcctggtata ttgtgcttcc gcactttctg
      541 ccggcggtca gctgtttgag tctgtgttcg gtattgatta tcatgtggca ctgaccatcg
      601 gtgcactggt agtactggtg tatacgtttc tcggtggatt caccgcggta tgtgtgacag
      661 actttatcca gggtatgctg atgctggtcg gtattctggc agtaccgctg ttcgcttatc
      721 attttctgac aaccggcggc actacattgt cagccggtct ggaagccagc ggtgctgaca
      781 gtgccaactt cctgaacctg atgaagaacg gtgacggcag caacaatatc gtcagcgtga
      841 tctccggtct cggctgggga ctgggatatt tcggtatgcc tcatatcctt gtgagattta
      901 tggcagtccg ggatgaaaag gaaatgacca agtccaaggc tacagccatc agctgggtgg
      961 cattatccct gggatttgca gtgtttatcg gtatcctggg cagagcttat ctgccggagc
     1021 tggtgaacgg caataatgag aaggtattca tcgagatgat caagaaggta tttaccgtgg
     1081 agatgcgggc tccgttcatt gcaggattgt tcctctgtgg tatcctggca gcgatcatgt
     1141 ccacggcgga ttctcagctg ttagtcagcg cctctagcgt agcggaagat atcttcaagg
     1201 gactgctcaa aaaggatgcg gatgacaaga cggttatgaa tgtgagccgt atcacagtac
     1261 tggtagtagc tgtgctggct tatatcatcg catggaatcc gaacaacact gtcatgggac
     1321 tggtatccaa cgcgtgggca ggtttaggtg ccgcctttgg tccgctggta gtgatgtcct
     1381 tatattggaa gagaaccaac ttcccaggag cggtagccgg aattgtcacc ggtgcacttg
     1441 ccgttatcat ctgggattac atccctctcg tgggcgggca gaccctgggc aaagctaccg
     1501 gattgtattc tctggtagta ggcttcgggc tgggacttat cgctatcatc gcagtcagcc
     1561 ttgctaccaa ggctccttcc gaggaaatgc tacaggagtt cgaggatgtg aaagcgaacc
     1621 ggctgtaaca gaccgttgat gaaataaata ttgacagaca aaaatactga tggacagcga
     1681 agacatctgc ctgtcagtat ttttatgtat ttgtgttggc gagggtatag aattcagaaa
     1741 caagctatct gtacccagca gaacatgcga aagcgggtat gtgagacaaa atcaggtcga
     1801 ttatacccac cggccttcgc ggacgcgggt atgtgagact aaaccaggtc gattataccc
     1861 tccacctttg tacatcgggg cacagataag gattttgccg taataagccc cnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naataagccc cgtcgatacc gggacatcgg
     2041 ggcgcagaaa gggattttgc cagaatacgc cccggcagca cctgagagca tccaggggga
     2101 tggcttttct tgtcataaaa aattattggg ctgtattata tgagttaagc gacaaaaata
     2161 tgattcattt gttgcttaac tcattagata taagaagaaa ctcacaaaaa ttcagtggcg
     2221 aacctgtgca aaacagcaaa aaagaattgt atctaaaaaa atacaatttt tttaaaaaaa
     2281 gtcttgcaat gcccgtaaat tcaatgtata ctaatccttg ctgtggcatg atagctgtga
     2341 agcgtgaggt tgccggttcc cagtaatgga gatcgggttt tccgtggagc gaatgtcaag
     2401 ttagaaaact gacggcaagt cactgtacca attaacatct gccttgagca gtaacaacgt
     2461 gtaagtttat gaccggagag aagaaagcgt tgcacaaagg tcgcagacac acacgggaaa
     2521 gtgtacagtc accgcttgtc cgtacaaaaa gtaagtatta acttacaaaa gtgcgaaaag
     2581 gaggcgactt tttttatggc aaatcaggta atgagaatta cattgaaagc ttatgaacat
     2641 cagcttgttg atgcatctgc caaaaaaatc atcgaaactg ttaagaagac cggagctcag
     2701 gtgagtggtc ccgtacctct tcctaccaag aaggaagttg ttaccattct gagagcagta
     2761 cacaagtaca aggactccag agaacagttc gagcagagaa ctcacaagag actgatcgac
     2821 atcatggctc ccactcagaa gactgttgat gctcttcaga gactggaaat gcctgccggt
     2881 gtttacatcg acatcaagat gaagaacaac taataagtaa gtttttcaaa gtaaagccaa
     2941 aacaacaaaa acctctactt agacgtaaga acacagtgcg atgaacaatc gccagggaaa
     3001 tgcgcgaagc gtatttctaa gtgtttcgca gaaacacttt gggttttgca agaaacactt
     3061 tggtccgcta tgtagaacac aggaggtaaa aatgaagaaa gcaattttga caacaaaagt
     3121 tggaatgacc cagatcttca atgaagatgg cgtgctcgtt cccgtaaccg tgcttcaggc
     3181 tggtccctgc gtggttactc aggtaaagac cgtggagaat gatggctaca gtgctgtaca
     3241 ggtaggcttc gttgacaaga aggataaggt tgttaacaag gatgccagcg gcaaaaagca
     3301 gatcgtacac agacatggtg ttaacaaagc agagcagggc cacttcaaga aggctggcgt
     3361 aagctccaag agatatgtaa gagagttcaa gtttgagaac gctgaggagt acacccttgg
     3421 tgcagagatc aaagctgaca tcttcgctgc aggcgataag gtagacgctt ccgcaatctc
     3481 caaaggtaag ggcttccagg gcgcaatcaa gagatttgga cagcacagag gacctatggc
     3541 tcatggctcc aagttccatc gtcatcaggg ttccaatggt gcatgttcct cccctagccg
     3601 tgtattcaag ggcaagggaa tgcctggaca tatgggttgt gtaaaggtaa ctgttcagaa
     3661 tcttgaggtc gtaagagtag atgctgagaa gaatctgctt ctggtaaagg gtgcagtacc
     3721 cggacctaag aaagctttag taaccatcaa agagaccgtt aaggttgagg catagtttag
     3781 gacgaaagga ggaccaatca gatggcaaac gtatctgttt acaatatgga aggcaaagaa
     3841 gttggtacaa tcgaattaaa cgatgcagtg tttggtgttg aagtgaacga gcacctggta
     3901 cacatggcag ttgtacagca gcttgcaaat aatcgtcagg gaactcagaa agcaaagacc
     3961 cgttctgagg tatccggtgg cggaagaaag ccctggagac agaagggaac cggtcatgca
     4021 agacagggtt caaccagatc tccccagtgg gcaggcggcg gcgtagtatt cgctcccgtt
     4081 cccagagatt actctttcaa gcttaacaag aaagagaaga gagcagctct gaagtctgca
     4141 ctgaccagca aggttcaggg aagcagcctg atcgtagttg atgagctgaa attcgacgag
     4201 atcaagacca agaacttcgc caatgttatg aataacttaa aggtggaaaa gggactggta
     4261 gtgatcgctg ataacgatac taacgtagtt atgtccgcta gaaacgtagc tgatgtagat
     4321 accaccctgg taaataccat caacgtttac gacgtaatga aggctaagac agttgtcctg
     4381 accaaggatg ctgttgcaaa gattgaggag gtatacgcat aatggctgat attaaatact
     4441 atgatgtaat cctcaaaccc gttattaccg agaagagcat ggccggcatg ggcgagaaga
     4501 agtacacctt cctcgttcac accgaagcta acaagtctca gatcaaagag gctgttgaga
     4561 agatgttcga aggtaccaag gttaagagtg ttaacaccat caatatggat ggcaagaaca
     4621 agagacgtgg aatggttacc ggcaagactg ccaagaccaa gaaggctatt gttcagttga
     4681 ccgcagatag caaagagatc gagatcttcg caggtctgta agatcgaagc tactgctgag
     4741 attcaggtat gcggcacata aagtgctcgc gcaaaaaatc atcgtaaagg agatacagta
     4801 atgggaatta agacatacaa cccctataca ccttccagaa gaaacatgac tggttctgac
     4861 ttctctgaga tcaccaagac ctctcccgag aagagcctgt gtgtttccct gaagaagaac
     4921 tccggccgta acaatcaggg taagatcact gtaagacatc gcggcggcgg ttccagaaga
     4981 aaatacagag taattgattt caagagatac aaggatggca tccctgcaac cgtaatcggt
     5041 atcgagtacg atcctaacag aacagcaaac atcgcgctga tctgctacga agacggtacc
     5101 aagtcctata tcctcgctcc cgaaggtctg accgacggaa tgaaggttat gaacggtgca
     5161 gaggccgagg tcagagtagg taactgctta cctctgtccg agatccctgt aggtactcag
     5221 gtacacaaca ttgagttata tcccggtaag ggcggccagc tggttcgttc cgccggtaac
     5281 agcgcacagc tgatggcaaa agaaggtaag tacgctactc ttcgtcttcc ctccggtgag
     5341 atgagaatgg ttcccctggt ttgccgtgca actgttggtg ttgtaggtaa cgttgatcat
     5401 agcctggtta agatcggtaa agccggacgt aagcgtcaca tgggcatccg tcccaccgta
     5461 cgtggttccg tcatgaaccc caacgaccat cctcatggtg gtggtgaagg taagaccggt
     5521 atcggtcgtc ccggccccag cacaccttgg ggtaaacctg ctcttggtct gaagacacgt
     5581 aaatctaaga aggcttccaa caagctgatc gtaagaagac gtgacggcag agcaatcaaa
     5641 tagttaagga ggaaagatca tggctagatc actgaaaaaa ggacctttcg cagacgcaag
     5701 cctgttaaag aaagtcgacg ctttaaatgc atcaggacag aagcaggtta ttaagacctg
     5761 gtcccgtcgt tctacaattt tcccttcctt cgtaggacat acgattgctg ttcatgatgg
     5821 tagaaagcat gttcccgtat acgttactga ggatatggtt ggccacaagc tcggtgagtt
     5881 cgtagcaacc agaaccttca gaggtcacgg caaggacgag aagaaatcca aggttagata
     5941 attttattat tgacctggag ctaaaaatat gctatctttt atagatggct gggagagcta
     6001 tgcgctctcc cgagggtctt ccctcgaaag aggaaagaag acgggatatt tttgtgcgca
     6061 gcacttagga aacacacggg tgcgttgcgc ggtttcagtt tgaaaggagg accaaatcta
     6121 tggctaaagg acatagatcc caaattaaga gagaaagaaa cgctcagaaa gatacaagac
     6181 cttcagctaa gctgtcttac gcaagagtat ctgttcagaa ggcttgtttc gtattagatg
     6241 ccatcagagg caaagatgta cagactgcac ttggtattct ggagtacaat cccagatatg
     6301 cttccagcct gatcaagaag ctgcttgagt cagcaatcgc taacgctgag aacaataacg
     6361 gtatgaatac cgagaacctt tatgttgcag agtgctttgc aaacaaagga cctaccatga
     6421 agagagtaaa gcccagagca cagggtagag cttatagaat cgaaaagaga atgagccaca
     6481 ttaccatcgt tcttgatgag aagaagtagg gaaaggagct attaaaagtt tatgggacag
     6541 aaagttaatc ctcacggcct gagagtcggt gttattaaag attgggattc caagtggtac
     6601 gctgatgcag agttttccga ttatctggta gaagactaca acatcagaaa gttcctgaag
     6661 aagaaattat acagtgcagg tgtttccaag atcgagatcg aaagagcatc tgacagagta
     6721 aaggttatca tctatactgc taagcccggt gttgtaatcg gtaagggcgg cgctgagatc
     6781 gaagtaacca agaaagagct cgccaaatta accgataaga aggttatggt tgacattaaa
     6841 gagatcaaga gacctgacag agatgcacag ctggtagctg agaacatcgc tcagcagctg
     6901 gagaaccgtg tatccttcag acgtgcaatg aagtcctgca tgggcagaac catgaagtcc
     6961 ggtgctatgg gtatcaagac ctgctgttct ggtcgtctgg gcggtgctga tatcgctcgt
     7021 gctgagttct acagcgaggg aaccattcct ctgcagacac tgagagcaga tatcgattat
     7081 ggttttgctg aagcaaacac cacctatggt aaagttggag ttaaagtttg gatttataaa
     7141 ggcgagatac ttcctacaaa aggaaatcag atggaaggga gcgataaata atgttaatgc
     7201 cgaagagagt aaaacgtcgt aaacagttcc gtggatccat ggccggaaaa gcttccagag
     7261 gtaacaccct ggcgtatggc gaatttggtc tggttgctac agaaccctgc tggatcaagt
     7321 ctaatcagat tgaggcagcc cgtattgcgc ttacccgtta taccaagcgt accggtaaag
     7381 tatggattaa gattttcccc gataagcccg taactgtaaa gcctctcggt actcgtatgg
     7441 gttccggtaa aggcgctgta gattgctggg tagcagttgt taagcccgga cgtgtaatgt
     7501 tcgagatcgc tggtgttccc gaagaggacg ctcgtgaagc attacgtctt gctatgcaca
     7561 agctcccttg taagtgtaag atcattgcta aggcagatgt ggaaggcggt gtgacagaat
     7621 gaaatcaaat aaatttgtag aagaattgaa gacaaagtcc gatgcagaac tggcacagga
     7681 gctggtagct gctaaaaaag aacttttcaa tttgagattc cagaacgcaa ccaatcagtt
     7741 agataatact gcaagaatca aagaagttag aagaaacatt gcacgtattc agactgttat
     7801 caccgagaag gctagagcgt aacgcgtggg tgcgtagaaa ggagatcaaa ccgtggaaag
     7861 aaatttgaga aaaacacgta ctggtaaggt tatcagtaat aagatggata aaactattgt
     7921 agttgcaatc gaagagcatg ttaagcatcc cctttacaaa aagatcgtaa aggacaccta
     7981 caagctgaag gcgcatgacg agaataacga gtgcaacatt ggcgatatcg taaaagttat
     8041 ggagacaaga cccctgtcca aggacaagag atggagactt gttgagatcg tagaaagagc
     8101 taaataaatt agcacgcgaa tattaagttt gtaagaaacc gcggaagcgg tgaaggagga
     8161 ataacatggt tcagcaggaa agtagactga aagttgctga taacaccggc gcaaaagagt
     8221 tactttgcat tcgtgttatg ggcggttcta ccagaagata tgcacagatc ggcgacgtga
     8281 ttgttgcttc cgttaaagat gcaacaccag gtggcgttgt aaaaaaaggt gatgtagtaa
     8341 aggcagttgt agttcgttcc gtaaagggcg ctcgtcgtaa agacggttct tacatcaagt
     8401 ttgatgagaa cgctgcagtt atcatcaaag atgacaagac tcccagagga acccgtatct
     8461 ttgggccggt tgcaagagag cttcgtgaga aacagttcat gaaaattgtt tccctggctc
     8521 ccgaagtatt ataaggaggt tgccacatgt ctacaatgaa aattaagaag ggcgacaccg
     8581 ttaaggttat cgctggtaaa gacttaggta cagagggcaa ggttctctct gtagacgtaa
     8641 agaatcataa agtcctggtt gagggcgtta acaagatcac aaagcatgca aagccttctc
     8701 aggcaaaccc caatggcggt attgttgaga aagaagctcc tattgatatt tcaaacgtta
     8761 tgcttgttgt aaaaggcaag accacaagag ttggcttcaa ggttgaagac ggcaagaaag
     8821 tacgtttcgc aaagtcaaca ggcgaagtga ttgactaatc aataggaagg aggaaatata
     8881 agtgagcaga ctgaaagatt tatataacaa tgagatcgtc gatgctatga ccaagaagtt
     8941 cggttacgct aatgtaatgg aagttcccaa gctcgacaag atcgttatca acatgggtgt
     9001 tggtgaagca aaagaaaacg ctaagatcct tgagaatgca gttgctgata tggaagctat
     9061 caccggacag aaggcagttc tgaccaaggc taagaattcc gtagcaaact tcaagatccg
     9121 tgagggcatg cccatcggat gtaagaccac tctgcgtggt gagaagatgt atgagttcct
     9181 ggatcgtctg gttaacctcg ctcttcctcg tgtccgtgac ttcagaggcg ttaaccctaa
     9241 cgcattcgac ggcagaggta actatgcact cggtatcaaa gagcagttca tcttccctga
     9301 gatcgagtac gataagattg acaagaccag aggtatggat atcattttcg taacaactgc
     9361 taagaccgac gaagaggcac gtgagctgct gagactgttc aacatgcctt tcgcaaagta
     9421 atcaaggagg taaattctga tggcaaagac atcaatgaag attaaacagc agcgcaaacc
     9481 taagttctct actcaggctt atacccgctg caagatttgt ggtcgtcccc actctgtact
     9541 gagaaaatac ggaatttgcc gtgtttgctt ccgtgaactg gcatacaagg gcgagatccc
     9601 cggagttaag aaggcttctt ggtaagagtg aaaagatttt ctaagccagc aaaatacgct
     9661 ggctaacaaa atctatattt cactcccaag agaattcctg gcggaattct ccgagacaaa
     9721 aagaaattct aaggcgtcga aaggcgtcga gtaagaattt ctaaaagttt tggttataag
     9781 aattatctta taatatagat ggtatataca aagtgtatgt acttagcatg attcaagtca
     9841 aataataagg aggaaactaa atcatgacaa tgagcgatcc tatcgcagat atgcttacaa
     9901 gaatccgtaa tgcaaatact gcaaaacacg acaccgtaga cgtaccttct tccaagatga
     9961 agctggctat cgcacagatt ctgctggacg agggatatat taagaagtac gacatcgttg
    10021 aagacggtgg tttcaagacc atccacatca ctctgaagta cggcgcagac aagaacgaga
    10081 agatcatctc cggcatcaag agaatctcta agcccggctt acgtgtatac gctggcaagg
    10141 aagatctgcc taaggttctt ggcggtctgg gtattgctat tatctctacc aaccagggtg
    10201 ttatcaccga caagaaggct cgtgagcttc aggttggcgg cgaagtatta gcattcgttt
    10261 ggtaaggatg aaaagatttt ctaagtcagc aaagtacgct gactaacaaa atctaaattt
    10321 cattcctaag agaattttcc gcaagcgtaa aattctccgt gggatgaaaa gattttagca
    10381 actgttgtaa gttagtaaat taggaggtac gggcatgtcc agaataggta gaatgccaat
    10441 cgcaattcct gcaggcgtaa ctgtagaggt tgcagaaaat aataaagtga ctgtaaaagg
    10501 tcccaagggt actctggtaa gagtattacc ctctgagatg gatattaaag tagaaggagc
    10561 agaagttctc gtaagcagac ccaacgatct gaagaagatg aagtctcttc acggtctgac
    10621 cagaactctg atccacaaca tggtagtagg tgttaccgaa ggtttcgaga agaagctgga
    10681 agtaaatggt gttggttaca gagctgctaa gtccggcaag aagctgaccc tgaaccttgg
    10741 ttactctcat cccgtagaga tgatcgatcc cgaaggcatt gaagctgtag ttgaaggcca
    10801 gaacgtgatc atcgttaagg gtatcgacaa agaaaaagtt ggccagtacg ctgctgagat
    10861 ccgcgacaag agaagacctg agccttataa gggcaagggt atcaagtatg ccgatgagac
    10921 catcagacgt aaagttggta agactggtaa gaaataatag ataaggagag tatgaagatg
    10981 gttagtaaag agtccagaca gttagtacgt gcaaaaaagc acatgaaaat tcgtaaccgc
    11041 ttcagcggca ctgctgaaag acctcgtctt gcagtgttca gaagcaataa tcatatgtat
    11101 gctcaaatta tcgacgatac agttggtaac actttagtat ctgcatccac tcttgagaaa
    11161 gagatcaagg cagagctgga gaagaccaat aacgttgatg cagcagcata tcttggaact
    11221 gttatcgcaa agagagcaat cgaaaaaggt atcaaagaag ttgttttcga ccgtggcggt
    11281 ttcatttatc agggtaaagt agcagcttta gccgatgcag ccagagaagc tggcctggaa
    11341 ttctaggaaa ggaaagagga atacaccatg aaacacacaa tcatagatgc aagccagtta
    11401 gagttaaatg ataaagttgt taccatcaag cgtgtaacca agaccgttaa gggtggtcgt
    11461 aacatgagat tcaccgcact ggtagttgtt ggtgacggca acggccatgt aggcgctggt
    11521 ctgggaaagg ctgtagaaat tcccgaagct attcgtaagg gtaaggaaga cgctgttaag
    11581 cacatcgtag agatcaatct ggatgagaac aattccatca cccatgattt catcggtaag
    11641 tacggttctg ctaacgtttt actgaagaag gctcccgacg gtaccggtat catcgctggt
    11701 ggtcccgctc gtgtcgtttg tgagcttgca ggtatcaaga atatccgtac caagtctctt
    11761 ggctccaaca ataagcagaa cgttgttctt gcaacaatct ccggtttaag ccagatcaag
    11821 gctcccgaag aagtagcaaa gaatcgtgga aagtctgttg aggaagttct ggcgtaagcc
    11881 ggatcttccc gttaagttga cagggccaac agaaaggagt agagtatggc agacaagaag
    11941 ttaaagatca ctctggtaaa gtccaccatc ggcgctgtgc cgaagaataa agcaatcgtg
    12001 gagtctatgg gactgcgtaa gatcggcaag tccgtagtac tgcctgataa tgatgcaaca
    12061 agaggacaga tccgccaggt gaatcatttg atcaaggtag aagaagcata atcagataag
    12121 gaggtgttag gaatggaatt atccaattta agacctgcag aaggttccaa acacagtgat
    12181 aattttagaa gaggccgtgg acacggttca ggaaatggca agaccgcagg taagggtcat
    12241 aagggacaga aggctcgttc cggagctccc agacctggtt tcgaaggtgg tcagatgcct
    12301 ttatacagac gtattcctaa gagaggtttc acgaacagaa acactaagga aatcgttgct
    12361 atcaatgtaa gtgctctgga gtgctttgac aacggtgcta ccgttgatgt aaacgcattg
    12421 gttgaagcag gaatcattaa gaaccccaga gatggagtta agatccttgg aaacggagaa
    12481 cttaccaaaa agcttgatgt aaaggtagat gctttcagcg catccgcaaa agaaaagatc
    12541 gaggctcttg gtggaactgc tgaggtgatg taatgcttac aacactgaaa aacgcattta
    12601 aaatcaaaga aatcagacag aagattcttt ttacactggg aatgttagtt gtgatccgta
    12661 tcggatcaca gcttcccgta cccggggtgg acacgaagtt cttttctcaa tggtttgccc
    12721 agcagacagg cggtgcgttc agtttctttg atgccattac gggcggttct ttcctgaaca
    12781 tgtctattct ggcattgaac atcaaccctt atattacatc ttccattatc atgcagctgc
    12841 ttacaattgc tattccaaag ctggaagaaa tgcagcgtga cggtgaagac ggccgtaaaa
    12901 aaatggttgc catcaccaga tacgttaccg tagcgcttgc attgatccag tccacagcaa
    12961 tggcaatcgg ttttggcaga cagggctact tgatcgagtt caacgctctg aatgtaatta
    13021 ccacgatcac agcacttacc gctggtagtg ctttcctgat gtgggtgggt gaacgaatca
    13081 cagaaaaagg tatcgggaat ggtatttcca tcgtactggt gatcaacatc atttcccgac
    13141 tgcctcaaga cctgagcaac ctgtttgagc agttcgtatt cggaaaagct ccggcaaccg
    13201 caatccttgc agtagttatc atctttgcaa ttattattgc catggtggta ctggttatca
    13261 tcttaaatga tggtgtgcgc aagattccgg tacagtatgc caagaaaatg cagggcagaa
    13321 agatggtcgg cggacagact tccaatattc cgttgaaagt aaatacttcc ggcgttattc
    13381 ccatcatttt tgcacagtcc attatgcagt tccccattat tatctgttcc tttatcggat
    13441 ataacggaac cggcgtatgg gcagagatcc tgaaaggtct gaattccggt tactggtgca
    13501 aacccagtca gccgatttat tccctgggac ttttgctgta cattgtattg attgtatttt
    13561 ttgcatactt ctatacatcc attacattta atcctttgat gattgccgat aacatgaaga
    13621 agcagggtgg tttcatcccc ggtatcagac ctggtaagcc taccagtgat tatctgaaca
    13681 agattctgaa ttatatcgta ttcatcggtg cgattggcct gatcattgta tctgtgatcc
    13741 cgtatttctt taacggtgtg ttcggcgcaa gtgtatcctt cggcggtacc agcctgatca
    13801 ttatcgtaag cgtagtactg gagaccatga agcaggtaga atctcagatg ctcgttcgca
    13861 actacaaggg attcctggaa gattagtagc ataacagtaa ccggatggtt tgtaatcatc
    13921 cggttatttt tgcattattg tattatagga aaatgtattg caaaaaagcc gcataaaagt
    13981 taaaatataa atgttggact gtatttgtag cttatctata gaaagagcag agcctgtagc
    14041 aacaggcaga taggagcaaa gatgaaaatt atcatgttag gcgcacccgg cgcaggtaaa
    14101 ggtacccagg caaagatgat cgctgaaaag tacggaatcc ctcatgtatc cacaggcgat
    14161 attttccgtg caaatatcaa gaacggaacc gaacttggta tggaagcaaa gaaatacatg
    14221 gatcagggac agctggttcc cgatgaactg actgttaaga tcctcttaga cagagtagca
    14281 caggatgatt gcaagaacgg ttatgtactg gatggattcc ccagaaccat tcctcaggca
    14341 gaagtactgg acaaggcact gactgagctg ggagatgcca ttgattttgc aatcgatgta
    14401 aatgttcccg atgagaatat cgtgaagaga atgtccggca gacgtgcatg cctgtcctgc
    14461 ggcgcaacct atcacattga gcatattcct cccaagaagg aaggcatctg tgacaagtgc
    14521 ggacaggaac tggtactgcg tgacgatgat aagcctgaga ccgtactgaa ccgtctgaaa
    14581 gtttatcatg accagaccca gcctctgatc gaattctata ccaagaaaaa tgtattaaag
    14641 accgtagacg gcaccaagga tatgaaggaa gtattcgcag atatcgtagc aattcttggc
    14701 taggaaaagt ccggatcact gatcagcagc agtaaggtcc ggaacagaaa ggtgaataaa
    14761 atggcagtaa ctatcaaatc acagcgtgaa attgagttga tgagagaatc ttgtaagatt
    14821 ctggcggatg tattcagcga gatggagaag gcagtacacc ccggggtatc cactctggat
    14881 atcaataacc tgggagagaa actgatccgt gcccatggct gcattcccaa cttcttaaat
    14941 tacaacggat atccggcatc tatctgtgta tccgtgaatg atgaagtcgt acatggtata
    15001 cctcacaagg atcatattct tcacgaaggt gatattgtaa gtctggatgc aggactgatt
    15061 tataaaggat accattccga tatggcacgt actttcccgg tagggcaggt aagcgaggaa
    15121 gcaagacttc tgatggagag gaccaaagga agcttttttg caggaataga gatggcaaag
    15181 gcaggcaatc atctgtatga tatctccaat gccattgatg cctatatcag gccttttggc
    15241 tacggaatcg taagagatct ggtaggtcac ggcattggta ccagcttaca tgaggatccc
    15301 cagatcccca acttcgcaca gcatagacgt ggactgaagc ttcaggccgg tatgacgctg
    15361 gcaatcgagc ccatgatcaa tatcggaaga ccggatgtgg catggctgga cgatgattgg
    15421 actgtagtta ccgaggatgg atccctgtct gcacattatg agaataccat cctgatcaca
    15481 gacggtgagc cggagatcct gaccatgtat taaggaacgg gtaacggcag acccggagaa
    15541 gaaaggtaag attttgatat gttaggagaa tttgcaactt caaaggccgg acacgataag
    15601 gacagactgt acatgatcgt cggtgaagag gaagaatggg tctatctgtg tgacggaagg
    15661 ctgcgcggtg tggaacatcc gaagaagaaa aagaaaaaac atatccagat catacattct
    15721 tcggcagagg atgcgttgat acaaagcatg caacagaacc ttccggggga gaaagacgag
    15781 atcaacagac gaatctgtaa gaccctggaa gcgtatctgt gtaataagta atataaagca
    15841 gtaaaagtag taaagagtag aaagctatgg aggaaattta tgtctaaaag tgatgtaatc
    15901 gaaatcgaag gaaccgttgt ggagaagctt cccaatacca tgttccgtgt agaactggaa
    15961 aacggacaca gagtactggc taccatcagt ggtaagcttc gtcagaactt catccgtatc
    16021 cttcccggag ataaggtaac actggaactg tctccctacg atctgagcaa gggacgtatc
    16081 atctggagag ataagtaaat accatctgga gagaaaatag ctatggctaa aaaaatacgt
    16141 aactttgctg ccattctggc ggtcagtgcg gtagtgggaa cgatactgtt agtactggta
    16201 ttcttgcttc cggtaggacc tatgagaaag aatgtggaga aaagtgtcgg ggatatgctg
    16261 aagaccggag acgagatacc ggaagatgca ttttcgcagt acctgtggaa aaacagagag
    16321 acctacaccg atgctattat ggtgcagaat gccatagaga gattgccgga caaaaatgcc
    16381 tacgaacacg ctatgtggat gtaccactat gatctggaag aggatgtatg gacaccggag
    16441 gattctctca aatccttctg tgaatcccat gagaacgtca ataacatgta tctccacatt
    16501 tatgcgagat actggcacgg ctatctttta tatttaaagc cgctcttact gttattttcc
    16561 tggcagcacg tggtatggct ggagctggcg gtgcagatcg cgcttatgat ctgggtgctg
    16621 gttacggcaa tccaaaagca aaatgcaggt gttgcggtcg tgactctggg aagtttcctg
    16681 tttatgaagc cggtgctggt tcttgtatca ctgaccatgt cagtgtgctg gatcttgacg
    16741 cttctggcgg tggaatacat gctgttacat catgacagac tgcacgaaaa aggacaatat
    16801 ccggaattct tcctgatcgt gggaattctg acgtcttatt ttgattttct gacgtatccc
    16861 atcgttactt tgggaattcc tctgtgctgt tacttcttat tagagaggga tcgcctgtgg
    16921 aataatatca aaagactgac cggcttcagt gtaagctggg caatcggtta tgccggaatg
    16981 tgggcagcaa aatgggtcat tgcagatctg acattacata ccggtacgat caaggatgct
    17041 atctggtcca ttatcggccg taccgaagct attggcggca gaccccggat gaacgggggg
    17101 ttctatgtca tcggactgaa tcttcaggaa tatccggcct atattggaat tgctgcagga
    17161 gtgctggcag cgatagcgat aggaatgctt gtgatgataa tcgtcacggg caaatggaag
    17221 gaaatatttg cacagctggt tcccgtagta ctcacagctg caattccctt cgtctggatc
    17281 attgcagtac aacatcattc agcattgcat gcgagattta ccttccgaat tttaagtgtt
    17341 gcagcggctg cggctatcac ttttatcgtt ttgaatgtaa agcaggcaaa aaaacaacaa
    17401 aaaagtcaaa aaatatttaa aaaagcgcaa taatacgctt gcaaaggaaa gaccgtcatg
    17461 ctataatgta agacggtctt ttttgaaagg agggtttaac atgaaagtta gatcatcagt
    17521 aaaaccgatt tgcgaaaagt gcaaaatcat caagagaaaa ggacgcatca gagtaatctg
    17581 tgagaatccg aagcacaaac agagacaggg ataatcaccg cttgatcgtc atttgatcag
    17641 gtatgtgagt tcttgtccgg ttattatgtg cggctgaatt gataatatca attttcataa
    17701 tagatttgga tagagcttac gggagattac ttgttgatac catgacgaaa agattttcta
    17761 aatttgtaga agacataaat taagaaaatc taagtttcgt caccaaaaag aaatcacaga
    17821 tttctttttg cggatgcaga catattcgtt atggaaagat cggatcgtgt ccgattgggt
    17881 gagagcccca atcaattagt aactattggg cgtacaatta acagaccaaa acgtacgcac
    17941 acacaacgaa agttgtgagc gcagaaatgt tgcgcaaagc gtaagagatt acgcaggaaa
    18001 atggaggaaa cgtaaatggc tcgtatcgca ggtgtagact tacctagaga aaaacgaatt
    18061 gagatcggtt tgacctatat ctatggaata ggcagaccta ctgctgacaa gctgttagca
    18121 gaagcaggtg taaatcctga taccagagtg agagatatta ccgatgatga ggtaaagaag
    18181 atcagtgaag ctatcgaaaa gcttggcgtt atggtagaag gtgatctgag aagagaaatc
    18241 gctctgaata tcaagagact tcaggaaatc ggctgctatc gtggtattcg tcatcgtaaa
    18301 ggacttcctg ttcgtggtca gaagactaag accaacgcaa gaactcgtaa gggacctaag
    18361 cgcacggtgg caaataagaa gaagtaattg agatcaaaat gtaactgtga gagggaagca
    18421 aatgtaagca tccccaggaa atgagaaagt aggttagttt aaaatggcta aaaaagttgc
    18481 aaagaaagta acaaaaaagc gtgtaagaaa aaacgttgaa cgcggacagg cacatattca
    18541 gtcttctttt aacaacacaa tcgtaacgtt aactgatgct gaaggtaatg ctcttagctg
    18601 ggcaagtgcc ggtggtcttg gttttagagg ttcaaagaaa tctactccat atgctgcaca
    18661 gatggctgct gaaacagcaa caaaggctgc tcttgttcac ggattaaagt ccgttgacgt
    18721 attcgtaaag ggtcctggtt caggacgtga agcagcaatc agagctctgt ctgcaagtgg
    18781 actggatgtc ttaagcattt gtgatgtaac tcctgtacct cacaatggat gccgtcctcc
    18841 taagcgtcgt cgcgtttagt gagaaatcgt cagaagacga agcaattaat tttaatgaag
    18901 taacattaga acaagttgga tgaaagtgcc gcattgcggc actgtaaaca accctcggaa
    18961 gtcacttccg aagtataaag aagggaaaat aaaaaaatgg cagtaaatcg cgtacctgta
    19021 ctgaaaagat gtagagccct tggccttgag ccttcttacc tgggctatga caagaagtct
    19081 aacagaacct ctgcaagagc aggcaaaaag gtatctgagt acggacttca gttaagagaa
    19141 aagcagaagg ctaagttcat ctatggcgtt ctggagaagc ctttccgtaa ctactatgag
    19201 aaggctgata gaatgaaggg catgaccggt gagaacctga tgatcctgtt agagagcaga
    19261 ctggacaacg tagtgttcag aatgggcttt gcaagaacca gaagagaagc aagacagatc
    19321 gttgaccaca agcatgtact tgtaaacggt aagtgtgtaa acattccttc ttacctgatc
    19381 aaggctggcg atgttatcga gatcaaggag agcgctaagt ccatgcagag atacaaggat
    19441 atccttgatg ttactgctgg aagattagta cccgaatgga tcgatgctga tgttgagaac
    19501 tttaaaggaa ccatcaagaa tctgcctacc agagagatga tcgatgttcc tgttgacgag
    19561 atgcttatcg tcgagttgta ctccaagtaa gttttatgta accccaatga acatttcctg
    19621 tggaatatta ttccatggga tattgttcgt aagaacatgt caacatataa agttcgcaaa
    19681 ggagggtatt ggcagtgttt gattttgaaa gacctaatat tgaggttagc gagatttcag
    19741 aagacaaaaa gtatggtaaa ttcgtagtaa aacccctgga aagaggctat ggtattactc
    19801 tgggtaactc cctgagaaga atcatgctgt cttccctgcc cggtgctgca gtgagccaga
    19861 tcaaaattga aggtgttctt catgagttca gttctattcc cggcgtaaag gaagatgtat
    19921 ctgagatcat catgaacatc aagagccttg cgatcaagaa caacagcgag accaacgagg
    19981 ctaagactgc atatatcgag tttgaaggcg aaggtgttgt aagagcttct gatatccagt
    20041 gtgatcagga tattgaaatt ctgaaccccg accttgtaat cgcaactctg agcggtaaag
    20101 ataccaagct ttacatggag ttaactatta caagaggccg tggctacgta agtgccgaca
    20161 agaacaaaca cgaagattta ccaattggcg taattgccat tgattctatc tatacacctg
    20221 tagagagagt taacgttacg gtagaaaata cccgtgtagg tcagagtact gatttcgaca
    20281 agcttacatt ggatgtatac accaacggaa ccctggctcc cgatgaggca gtcagcctgg
    20341 cagctaaggt gctcagcgag catctgaacc tgttcatcga cctgtccgaa aatgctaaga
    20401 ccgctgaggt tatggtagag aaggaagacg acgagaagga aaaggttctg gagatgagta
    20461 ttgacgagct ggagctttcc gttcgttctt acaactgctt gaagagagca ggtatcaata
    20521 ctgtagaaga gttaaccaat aagacttcag aagatatgat gaaggttcgt aacctcggac
    20581 gtaaatcttt ggaagaagtt ctggctaaac tgaaagagct gggattacag ctgaacccca
    20641 gcgatgagaa ctaatttcag ctgaacataa aaagttagat ttccgtatcc ggtaagccca
    20701 aagtgttcgg atgcggttta tctctaaatg gcaaaaaaca acgcattcgg taagtaagtc
    20761 caaagtgcat ggccggatgt accatgaatg gagaaaggaa aattatcatg gcaaagtaca
    20821 gaaaactcgg cagaacatct gcacagagaa aggcattatt aagaaatcag gtaactgcag
    20881 tgatcaacaa cggcaagatc gttaccactg aagctaaggc taaggaagta cagaagatcg
    20941 ttgatggact gatcgctctt gcagtaaaag agaaggataa cttcgaaact gttaaagtta
    21001 ccaccaaggt tgcccgcaag gacaaggacg gcaagcgcgt taagcagatc gttgacaagg
    21061 agaccggtaa ggtcctggct gagtcccatc gcgataagga tggtaagctg gttaagatcg
    21121 agaacggtgt taccgttact gtatacgacg aagttgagaa ggagatcaag aaggatctgc
    21181 ctaccagatc tcatgcacgt cgtcagatgc tgaaggttct gaaccctgta gttgaggttc
    21241 ccgctgatgc agcaggcaag aaaaagaaca ccaaggaagt tgacctggtt gcaaagctct
    21301 tcgatgagta tgctcccaag tatgctaccc gtaagggtgg ttacaccaga atcgttaaga
    21361 tcggtcagcg taagggtgac gcagctatga ctgttgttct ggagcttgtt taagctacga
    21421 gcaaagtaag gataaaaaga gacaaccgct cgggaatgct tgcattcccg agcggttgtt
    21481 ttttttttag acttatttgg cgacagccgt acaacaaggg gatgctccgc attcccgagt
    21541 tgctttgcgg attactccac caccttctgg aagtgctggt aatccttgca ggagttccag
    21601 tgacctcccc agatgaagcc atgttcttta aataactgat agcacagatc gttttcgtca
    21661 attttgtagg ggaaggaagc ggttctgtct gcataggcgg aagcgtttgc aggagatacc
    21721 ctttcactgc catccttatt gtatgtaata taaggattgt aaaagggatt gatgtctatc
    21781 gctaatccca gggcatgctt tgacagggat gaggtccctt ctaccggacg ataattgaaa
    21841 caggaggtat tattatcctc catggaggcg gtgtcgtctc cgtcgtactc atcgatcaat
    21901 aagacttttt ccagttgata ttcattacaa tacagttcat aaaagatctc cgtcaggtcc
    21961 tgcgcgatcg ccttgttaca gatcaattcc ccttctgcag gatttccttc aaaattataa
    22021 tgccttatat gtacatagcg cagatcgtca tagcagattt cgacagattt caacaggtct
    22081 tgtgaggctt ctccggagtt atccacagta gcaggataag aaacacctgt tatatatctc
    22141 tgtaatttct cggataacgg ttcataataa aagccttccg acagggtgat ccgctggtcg
    22201 gtcaatagct ctccgttcag ggaatatccg gtcaaaacag tagtttctgc catggaagat
    22261 ccttcctgca agttttctgt cgcatttgca ttggaattgt cgacggccgg tgtaggttcc
    22321 ggggtaggtg atggctctga tgtctgataa gccagggaag gattgtccgt cgcataatcc
    22381 cccagagtgt aggaactgtg ctgggatact cttagcagtc ctccggcaac gacagtgatg
    22441 agcgcaagaa tacccaccat cttccatatt ttattgcgat cgatcggatt catagaatgc
    22501 ctcctcgttt tattttctga aatgttctgc aatagaaaga tacaaccata actataaagg
    22561 tttttggaag gcagtacaag agattttgaa aaagtcgggt cagttccctg ttaagtgttg
    22621 taaatctgtg cataatctag taaaatagga acgatatctt gcaaaagcag aagatagctg
    22681 attctccggc actaagcgaa tatggaatgg agattattta tggagatcat taaagcaaat
    22741 aatgtagtat atgaatatat tcgtcgggat gaagagggaa atgtggaagg gatcacaaga
    22801 gcagtgaatg atgtttctct tgatatagag cagggacagt tcatagccat cctcggacat
    22861 aacggttccg gcaagtctac tctggcaaag catatgaatg ccatcttaaa tccgacagag
    22921 ggaacgctct gggtcgacgg tatggacacg aaggatgagg acaacatctg gaaggtccgt
    22981 cagacagccg gcatggtgtt tcagaatccg gataaccaga tcataggaca ggtagtggaa
    23041 gaagatgtgg gatttggccc ggagaacatg gggatcccca cggaggagat ctggcagcgt
    23101 gtggaagaga gcttacgtgc ggtaggtatg tacgaatatc gaaagtattc cccgaataag
    23161 ctgtccggtg ggcagaagca gagagtgtct attgccggag tactggctat gcatcccaaa
    23221 tgcattgtat tggatgagcc gactgccatg ctggatccca gtggacgtaa ggaagtcatc
    23281 cgtgcagtcc gcggattgaa tcaggtggaa ggcgtcacgc tgatcctgat cacccattat
    23341 atggaagaaa ttattcatgc ggacaaggta tttgtcatgg atgaggggaa aattgccatg
    23401 cagggtaccc cccgggaaat cttttcacag gtggagcagc tgaaaaagct acggctggat
    23461 gtgccgcagg taacgctgtt atcgtatgaa ttgcagaaac gcggaattcc gctgccgaat
    23521 ggcatattga caagagaaga gctggtcagt gcactgggct atggaaaaac cgacaggaaa
    23581 tagtggcggg gatggcagcg aatgaaagta ctcgggaaag gatcgaaaaa atgtctatca
    23641 tattagatca tgtgaattat gtatacggtg cagatacacc gctggcagtt catgccctga
    23701 acgatgtctg cctgaagata ccggatggac agttcatcgg tattatcggg cataccggtt
    23761 ccggtaagtc cacgctgatg cagcatatga acggattgat ccgtgctacc gacggacata
    23821 tctattttaa cggagaagat atctacgaca aggattatga tatgaagaag ctccgcagca
    23881 aggtaggact ggtgttccag tatccggaac atcagttgtt tgagatcgat gtattcagcg
    23941 atgtctgttt cggaccgaag aatctgggac ttgataaaaa ggaagtggag cttcgtgcat
    24001 acgatgctct gaaaaaagta ggactgccgg atgaactttt ctatcagtcc ccctttgaat
    24061 tgtccggtgg acagaagcgt cgtgtggcga tcgccggcgt gctggccatg aagccggagg
    24121 tactgatcct ggacgagccg acagccggac tggatcccaa gggaagagat gagatcctgc
    24181 agcagatcaa aaaactgcag acagagaccg gacttaccat cctgctggta tcccacagca
    24241 tggaagatgt ggcggaatat gtggaccgca tcattgtaat gaacaagggc agtgtcatgt
    24301 atgacgatac gccgagagaa gtgttcagac attacaaaga attggaagaa gtaggactgg
    24361 cagcaccgca ggtcacctac atcatgcatg agctgaaagc acggggcgcg gatgtggatg
    24421 tagatgccac aacgatcgaa gaggctgcgg aagaaattgc aagagtctgg aaacagaaca
    24481 atcagaagta atgatttgaa ctgcaatgct caaattgcag catgagcaga gagaagatac
    24541 gagttctgac ccgacaggag ccgggcagaa actctggtga ggagcaatat gcttagagat
    24601 attacattag gacaatatta tcagacagaa tccgtgatac acaggatgga tcccagagtc
    24661 aagctgggag gcaccctcct gtttatcata tcactgttct tttttaaaaa ttacgcggga
    24721 tatgtgatcg cagccctgtt tttggggatg gtgatccggc tgtcaaaggt accgttcaag
    24781 ttcatggtaa aaggaatgaa gactatcgtc atgctgatga tgatcacagt ggtattcaat
    24841 ctgtttctga cacagggaac accgttagtg acgatctgga agctgaccat tacgcaggaa
    24901 ggactgaaaa tggccatttc catggctgtc cggctgacac tgctgattat tggatcatcc
    24961 gtgatgacac tgactaccac gccgaataat ctgacggacg gtatggagaa aatgatggga
    25021 cccttaaaag tattcaaggt gcccgtacat gaagtggcca tgatgatgtc cattgcgctt
    25081 cgtttcattc ccattttatt agaggagacg gataagatca tgaaggcaca gatcgccagg
    25141 ggagcggatt tcgagagcgg caacattttc aagcgtgcca aggccatggt gcccctgctg
    25201 gtgcctctgt ttatttctgc gttccgcaga gccaatgatc tggctatggc tatggaagcc
    25261 agatgctatc gcggtggcga gggcagaacg aagatgaagc cgttagttta tcataagaga
    25321 gactatattg cgtacggctg cattgtggct tatatggtgg tcagcattgt ggtgggacgt
    25381 ctgtcgtttc tgccgtaaaa tgcagttgtg gcagtagcag cgggcgatat gctgaaaggc
    25441 ctggaaaaga caggacagaa gacatatgac aatgaacaga tgattagaga gaaagcgggt
    25501 gagacacagt ggaagcggaa aagggaacac aactggaaaa aacaataaaa cgtgtccgcc
    25561 tgaccgtggc ttatgacgga acgaattatc atggctggca gatccagaat aacggtatta
    25621 cgattgagag tgagctgaac cgctgtctga cggatctgct gagagaacct gtcgaggtca
    25681 ttggcgccag ccggacggac tccggggtgc atgcgttggg gaacattgca gtattcgata
    25741 ctactagccg gatgccggca gagaaaatct cttacgcatt gaaccagaga cttcccgagg
    25801 atatccgtat ccagaagtct gaacaggtgg aaaatgactg gcatccccgg aaatgcgaca
    25861 gccgcaagac ttatgagtac cggatctacc gggcacagtt ccccatgcca gtgaaacgtt
    25921 tgtattcgct gtttacttat tatgaactgg atgtgaaccg gatgcaggaa gctgcggctt
    25981 acctggaggg agaacatgat ttcaaaagct tctgccagac cggtgcccag gtggaatcca
    26041 ccgtacgcac catctattcc gtggaagtgg aagagcaggg agaaaatgat ctggtgatcc
    26101 gggtctgtgg taatggcttc ttatataata tggtaaggat cattgccgga accctgttag
    26161 atatcggaca gggcaagcgg gaccccatgg atattttcac gatcctcgag gcaaaagacc
    26221 gcagcacagc ggggcctacg gcaccggccc atgggctgac tctgatgaaa tacgagtttc
    26281 tgtgatcgtt cagcctgcgc cattcgcaaa gtcgcattat gacgctttcc gtcactacgt
    26341 gactatcttt gctcatatac cggcgcccgg ctcatagaac gtcaaaacag tgctttgtgt
    26401 gcaagcaccc aatcgctgat tctgccgttc tatggctgaa ctataaaaag tttaaattta
    26461 atcttgacac gcattgagtg gtatcatata atatatgact gtgacagtgt ttcaatatgc
    26521 tgagtcaaag ccccgacgaa gcattgaaat atagtattca accgttttgg aaagaagtaa
    26581 aatgtagccg gacatctgca gattaccatc ggaccagaac actgaaaaag tcgatcatga
    26641 atcggcgaaa gtaatatgga gggaacataa tgaaaacatt tatggcttgt ccagctacaa
    26701 tcgatagaaa atggtatgta gtagacgcta ctgacatgac cttaggccgt ctggcatctg
    26761 aggttgctaa agtgctgaga ggcaagaata agcctatctt cactcctcac atcgactgcg
    26821 gtgacaacgt tatcgttatc aatgctgaga agatcaaagt aaccggtaag aagatggatc
    26881 agaaggtata ttatcatcat tccgattacg ttggtggctt aaaagaagct accctgagag
    26941 aaaagttagc aaagaagcct gagcaggtta tcgaacttgc agttaagggc atgcttccca
    27001 agggaccttt aggaagacag atgttcacca agcttcacgt atatgcagga cctgagcaca
    27061 agcatgaggc tcagaagcct gaagtactga cattctaaag gactgagagg aggaaaataa
    27121 atcatggcta aaacagaaag atactacgga accggtagaa gaaagaaatc catcgccaga
    27181 gtatatttag tacccggtaa tggtaatatt acaatcaata agagaagcat tgatgagtac
    27241 ttcggtctgg agactctgaa ggttatcgtt cgtcagcctc tggcagctac tgagactgct
    27301 gagaagttcg acgttctggt taacgttcac ggtggcggat acactggcca ggctggtgcg
    27361 atcagacacg gtatttccag agcactgctt caggtagacg gcgatttcag acccgttctg
    27421 aagaaggctg gattccttac cagagaccct cgtatgaagg aaagaaagaa
//
LOCUS       DAWDHD010000016        26733 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_145_length_26733_cov_11.714746, whole genome shotgun sequence.
ACCESSION   DAWDHD010000016 DAWDHD010000000
VERSION     DAWDHD010000016.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 26733)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 26733)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..26733
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145_length_26733_cov_11.714746"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    18004..18103
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         7611 a         5539 c         7259 g         6224 t
ORIGIN      
        1 tggtggtcat ccgctacgaa ggccccaagg gaggccccgg catgcgtgag atgctgaatc
       61 ctacttctgc catcgcaggc atgggactgg gttccagcgt ggcattgatc acggatggac
      121 gtttttcagg agcatccaga ggagcatcca tcggacatgt atcccctgag gcagcagtgg
      181 gcggcccgat cgcactggtg gaagagggag acatcatccg tatcaatatc ccggagaaca
      241 agctggatgt actggtatct gacgaagaac tggcagcgag aagagcaaaa tggcagccca
      301 gagaacccaa gatcaccaca ggctatctgg caagatatca tgaactggtg accagcggca
      361 accgtggtgc ggtactggaa gcaccttctc agaagaacta agcaatcatt aaataaccgg
      421 agggattttg aaatgcagtt aacgggatcg gaaattctgg tagaatgtct gaaggagcag
      481 ggggtagata ccgtattcgg ttaccccggc ggagcaatcc ttaatattta tgacgcactt
      541 tataagcaca gtggggagat caaacatatt ctgacttccc atgaacaggg ggcggcacat
      601 gcggcggacg gctatgcgag agctacggga aaagtaggtg tctgcatggc taccagcggt
      661 cccggcgcca cgaatctagt cactggtatc gctacggctt acatggattc tgtaccgatg
      721 gtagcgatca ccgccaacgt taccttgcct ttactcggta aggacagctt ccaggaggtg
      781 gatatcgccg gcgtgaccat gcctattacg aagcatggct atattgtgaa aaatgtggag
      841 atcctggcag ataccattcg caaggcattc catattgcca gaagcggcag accgggccct
      901 gtgctgatcg atattacaaa ggatgtgact gcggcaacct gcgagtatac acctgtgacc
      961 attcagccgg aggaaagagt gacccgctac accagagaag aactgatgct ggtagcggaa
     1021 atgatacata aggcaaagaa gccgtatatc tatctgggcg gcggtgctat tatctccgga
     1081 gcaagtgcag aggtggcaga gttcgcggaa cttatcgatg caccggtatg cgataccctg
     1141 atgggaaaag gcgcttttga cggcagaagc gagcgctata caggaatgat cggtatgcac
     1201 ggcaccaaga cttccaatct gggcgtcagt gagtgtgacc tgttagtagc actgggagcc
     1261 agattctcag accgtgtgac gggtaacccg aagaagttcg cagagaacgc aagaatcatc
     1321 caactggatg tggatgctgc ggagatcaac aagaatatcc gtgtggatgc atctctggta
     1381 ggagatctga agaagactct gaccgaattg aatgcctgcc tgtcgaaaca ggaacatccg
     1441 gagtggatgg cgcatataat ggaactgaag gaaaaatatc ccctgaaata cgatgacgaa
     1501 aatctgtcct gtccttatat catgcaggag attgaccgtg taacgaacgg agaagccatc
     1561 atcaccacag acgtgggaca gcatcagatg tgggcggcac agtattatca ttatacgaag
     1621 ccccgtacct tcctgtcttc cggaggatta ggtaccatgg gttacggtct gggagcctgc
     1681 atcggggcgc agatcggaca gccggataag gtctgcatca acattgcagg cgacggatgt
     1741 ttccgcatga acatgaacga actggctact gccagccgtt acaacattcc catcatccag
     1801 gtggtcatca acaaccaggt gctgggcatg gtaagacagt ggcagacact gttctatggg
     1861 aaacgctatt cccagacagt actgaaggat aaggtggatt tctgcaaggt ggcagaagga
     1921 ctgggctgta ccgccatccg cgtgaccaaa aaggaagaag tggcaccggc catcgagaag
     1981 gccattgccc tgaaagcacc ggtactgatc gagtgcatga tcccggagga tgataaggta
     2041 ttccccatgg ttccggcagg agcccccatc agcgaagtgt ttgacggaga tgatttgaaa
     2101 aggcagtcct gatgaaaaag acgacgaaaa gattagtttt ggcagtactt ttatttttgc
     2161 tgttattact gaccggatat tttattctgg cattttatta cagagaaggc ttctctgtaa
     2221 atacctggat caacggtgtc tactgtaccg gaaaaacggt ggaggaagtg aattcggagc
     2281 ttttgttgcg tacagaggct ccgaatattg tcatcgtgga cagagagggg acagagtata
     2341 caatctctct tgcggaagca gattatcagg cagattacag taacgtactg gaacattacc
     2401 ggcaggaaca gaatccgttc ctgtgggtgg acaatgttct gtttcactcc cggagggaac
     2461 tttctcccac agtgaccgtg gatgtggatg ggcttcggag agcttatgat tctctggaat
     2521 ttgtacgggc cgaacaacag aagaacaagg attacagcct tgcccgggat acgtcgggcg
     2581 agtacgtctt cacggatgga cttgcgggac gcattgactt ggagaaggct tttttggcgt
     2641 tggagaacac agtagaaaac gggcaggaga cgttaaacct gtcagatagt ggctgttatt
     2701 atgacatcac tccaaatgag aaccagaaaa ctctgcggaa tctctggaag gagattgaac
     2761 gttatcagag ctgtgatatt gtctattgct ttggacagga gagacatccg gtgacggcag
     2821 cggactgtgc ctcttttctg gtgacggaga atggtctgcc ggtgacggat cagaccggta
     2881 actttgtact ggatgaagaa gccgtgacgg cgtatgtaga gcagctggcc gaaaagtatg
     2941 acggttatgg ccgtaccaga caattccaca gtaccagagg agatgtgatc accatcgagg
     3001 gcggcaccta tggaagcaag ctggatcaga aaaaagagac tgcatacctg atggagcatc
     3061 tgctggatgc cggtgtacat acggggacac agcagtccca tgttcccact tacgagagag
     3121 aagctttctg ctatggcagg gatgacatcg gagataccta tatcgaagtg gatatgacac
     3181 aacagaaaat gtactattat gaaaaggggg aactgcggct ggagacggat gtggtgaccg
     3241 gcaacatgcg cagacgtatg ggaacaccgg agggggtgaa tttcgtctat aacaagcaga
     3301 aggacagagt actgcgggga ccgggctatg cctcccctgt gaaattctgg gtacctgtaa
     3361 agggcagcat cggtatccac gatgccagct ggagaaaaga gttcggcggg gacatctacc
     3421 agactgccgg ttcccatggc tgtatcaata caccgaagga caaaatggaa gaattgtacg
     3481 acatggtaca gatcggtacc cctgtcgtaa tgttttatta ataaaacaga cattttctga
     3541 caaaatgtgt gtgggatgcg aacattttga aaataattta acaatgtagt tgactaaagc
     3601 aaatgaaaag cttattctat acctatgctt tttataaaaa atttagaaaa tatcgaggag
     3661 gtctttgaaa atggcaagag tgtataactt ttccgcaggc cctgcagtac tccctgagga
     3721 ggtattgcag gaagcagccg atgaaatgct ggattataga ggaaccggca tgtccgtaat
     3781 ggagatgagc catcgatcta aagcctatga tacgatcatt aaggaagcgg aagctgatct
     3841 cagagaactg atgaatatcc cggacaacta taaggtactg tttttgcagg gcggtgcgag
     3901 ccagcagttt gccatgatcc ccatgaacct gatgaagaac cgtgtggcag actacatcgt
     3961 aaccggtcag tgggcgaaga aggcatatca ggaggcttcc ctgtacggca aggcaaataa
     4021 gatcgcttct tctgaggata agaccttctc ttatattccc gattgctccg atctgcccat
     4081 cagcgaggat gccgattacg tgtacatatg cgagaataat accatctacg gtacgaaatt
     4141 caagaccctg cccaatacca agggtaagcc cctggttgca gatgtatcca gctgcttttt
     4201 atccgagccg gtagatgtga caaaatacgg tgtgatctat ggcggcgtac agaaaaatat
     4261 cggacctgca ggtgtggtta tcgtgatcat ccgtgaggat ctgatcactg aggatgtgct
     4321 tcccggtaca ccgaccatgc ttcgttataa gatccatgca gatgcggatt ctctgtacaa
     4381 tacgcctccc gcatacggca tctacatctg cggcaaggta tttaaatggc tgaagaagat
     4441 gggtggtctg gaagcaatga aggagcgcaa cgagaaaaag gcgaagatcc tgtatgacta
     4501 cctggatgaa agcaagctgt tcaagggtac cgtccgcaag gaagacagat ccctgatgaa
     4561 tgtcccgttc attaccggta atgaggagct ggatgccaag ttcgtaaaag aggcgaagga
     4621 ggcaggcttc gagaacctga aaggtcacag aaccgtaggc ggcatgagag caagtatcta
     4681 caatgcgatg cccatcgaag gtgtggagaa gctggtagag ttcatgaaga agtttgaagc
     4741 ggagaatgct taagggggag aaaactatgt ttcagatcaa ttgcctgaat cccatttcca
     4801 aagtgggact ggatcgtctg acagcagact ataaagtaac cgataatata aatgacgcag
     4861 agggcgttct ggtccgcagt gcttccatgc atgagatgga gctgccggaa aatctactgg
     4921 cagtagcgag agccggagcc ggtgtcaata acattccgct ggataaatgt gcggaaaagg
     4981 gtatcgtagt attcaatacg cccggtgcca acgccaatgg cgtaaaggaa ctggtgatcg
     5041 ctggtatgct gttagctgcc cgtgatgtgg taggcggtat cgaatgggtc aaggcatcca
     5101 aagacaacgc tgacattgcc aaagccgcag aaaaagaaaa gaagaaattc gccggtacgg
     5161 aagtaatggg caaaaagtta ggtgtcatcg gtctgggtgc tatcggcgtt aaggtagcca
     5221 atacagcagt aagccttggc atggaagtct atggctatga tccttacgta tccgtcaatg
     5281 ctgcatggaa tttaagccgt tctgtcaagc atgtgatgaa tgtggaagaa atttatgcag
     5341 actgtgatta tattacgata catgtaccgc ttctggattc cacaaagggt atgatcaata
     5401 aggaagccat cagcaaaatg aaagatggcg tggtcctgtt aaactttgca agagatctgc
     5461 tggcaaatga ggcagatgtc ctggaggcat taaagagcgg caaggtagcc cgttatgtat
     5521 ctgattttcc gaaccctacg acagcaggac agcccggctg tatcgtgatc cctcatctgg
     5581 gcgcaagcac cgaggaatcc gaagataact gtgcgaagat ggctgtggaa gagatgatgg
     5641 attatctgga gaacggcaat atccgcaaca gcgtaaatta tcctgcctgt gacatgggcg
     5701 tatgcagtca ggccggaaga attgcaattt tccataaaaa tattgccaat atgctgaatc
     5761 agtttaccgc agtgatcggt gagaagaata tcaatatttc cgatatgacc aacaaatccc
     5821 gcggtgaagt ggcatatacc atgctggata ttgaaaatgc ggcaacacag gatctggtag
     5881 aatccttaca gaagattgac ggcgtcttcc gcgtacgtgt ggtaaaatag aatatcaaat
     5941 aaaacatgca ggaaagttac agacaggcat tggatggaat tcatccggtg cctgttttac
     6001 gtttatgccg gaaacgcaga ctagcaaaat tataggtaaa acctgtgcaa aatgccagaa
     6061 aattacagat tttttcccgt aaaagcgttg tatgctatga aagaatctgt tatacttggc
     6121 atggatcaaa gacgtgagcc acaagagaaa gaaggaacaa aacagatgaa atttgatatg
     6181 cattgtcata caaaagaagg atctatggat ggaaaggttc ctattgacga ttttattgcg
     6241 gaattgatcc gcaagggttt tgacggtatg ttggtcagtg atcataattc atataatggt
     6301 taccgcacgt ggaaacgaaa catcaaggat caaaaatata aagattttgt agtgctgaag
     6361 gggattgaat acgataccat tgatgccggg catatcctgg tgatcatgcc tgagaatgtt
     6421 aagctgaaaa ttctggagct tcggggattg ccggtacaga ttctgatcga tatcgtgcat
     6481 aagaacggtg gaatattagg gccggcacat ccctgcggag agaaatattt aagcttcacc
     6541 aataccctga agaagcggaa tcagatggcg gtgatgaatc agtttgattt tctggaaggc
     6601 ttcaatgcct gtgagagtgt agagagcaac cattgcgcaa aggtactggc ggaaatgtac
     6661 gcactgccaa ccttcggtgg aagcgatgct cacaaaacgg actgtatcgg gatggcatat
     6721 accgatatac cagagaagat cagttgtgaa tccgatttga tccgggcagt gaagctggga
     6781 gaagggatca aatgcggagg ctcctattac cgcggcacta ccaaggagaa gatcggaaaa
     6841 gccaataata ttctggtaca gtccttctgg ttctataaca gactgggaag cctgtaccgg
     6901 catcacagaa gaaaagtgga aatgcataag atggtatagg tcccctggac ctatactttt
     6961 ttatacacat cgtcatgtga tgtcggggtc aaaaaccccc gactttgatg cctacggatt
     7021 gttccgtgct acgcactccc acttctccgc tccgcttcgg tccgtgctaa acatgtgcca
     7081 ctggcacata gcaccctacc ctctattcgc atatcgtggg attatcatcc cacttttatg
     7141 ctcatatcgg ggcgggtcat aaggtctttc acccatttat gagcaagctc atgtgggctt
     7201 agaccttttg accctggcat cgtaatctga gttcataaat tcttaagatg cttttactat
     7261 agaaataggg tataatgtgc atagataagt aagaaatatg gattgctccg tagaacttgc
     7321 tgtcaggggg aacccgtagg attacagacc gtaataaaga ggagaaaaat gtatgagaaa
     7381 aaatttaagg ggcagaaaag caactgcgat tgctctggca tccgttatgg caatgtcgct
     7441 tgcagcctgc ggcaggcagg atggatcgaa tcaggtggga cagacagaac agaaggaata
     7501 tgtatatgtt cccgaatacc tggagttgga tgataatacg aattccagct acagtaacat
     7561 gacagtacag ggagataagc tgtattatac aaattaccaa tgggatgaag cttccggtga
     7621 gagcaaggtc gtgcttggaa cttattcgct gacagacggt tcccgggagg atctgccgat
     7681 caccattaat gcggacggcg gtggaatcta ttccatgcag gcagacgcgg aaggtaatat
     7741 ttatactgcg gaatttgagt ggaatgccac agaaggtgat gacgcttata cacagcagac
     7801 gactgtgttg cacaagtacg atgcatccgg tacggagctg gtgacacagg atatcacgga
     7861 tatcatgcag caggatgaga acaattccta tgtgggcagc atgtgcctgg acgaccaggg
     7921 cagattttat atcagctctg attcattgat ccggctgttt ggcagtgacg gacaatttca
     7981 gggagctgtg cagacggaca gccagtggat ccagggcatg ggaaaggcaa aagacggcaa
     8041 ggtatacctg gcttattatg accaatccgg caatgtgaag ctatcccaga tcgactttga
     8101 cggcaaggca ttgggacaga catatgataa cttcccgaac acgaacggca acggaggatt
     8161 atgtgccgga atagaaaatg atctcctggt gaatacggat actgccctgt atgattatag
     8221 tctcgcagac cagaagacta ccgaagtact gagctggctg gacagtgata ttaacggcag
     8281 ttatgttacc tatgccgcag ccaccgcaga cggtaagatc ctggcagtgg tcaatgactg
     8341 gaacacaggg gaaactgacc tggtgaagct gaccagaacc aaggccagtg aggtagcaca
     8401 gaagtcccag atcacgatcg gaaccctgta tacctcccag agccttcagg cagcagccgt
     8461 agccttcaac aagcagagta atgaatatca tgtcaatatt aaaacatata ttgatgataa
     8521 taactggacc gaaacttcct gggcggatgg cattacagcc atgaacaatg atattacctc
     8581 cggagccggg tgcccggata tcctggatct gtccaacctg gatgtcaagg aactggcttc
     8641 caagggtgta ttcgaggata tgacacctta tctggagaag agcagtgtat taagcaagga
     8701 cgatttcttt gaaaatattg tagacagtta tacctttgac ggaaaactgg tgggaattcc
     8761 gaagagcttc accttaaaca cgatcgtcgg caagacctcc gaagtaggtg ataagaaggg
     8821 ctggaccatt gacgatatta tagcctatgc aggccagcac gaaggtgctt ccctgttcga
     8881 aggcatgacc aagagcggta tgctgtacac actgttagcc tatgatctgg acagctatat
     8941 tgactgggag acaggaaagt gcagctttga ttcagaagat ttccagaaag tactggaatt
     9001 tgtcaatacc ttccccgaag agtatgactg gcagaatgat gacagatcta caccggcgaa
     9061 gatccagtcc ggtgaagtac tgttgaatat gacaggcatc tatcagctga acagtattca
     9121 ggaggaggaa gctatgttcg gtgagccggt aacctatatc ggatatccta cctccggcgg
     9181 tggcagcggt acttatatgc aggccagtga gctgtatgcg attaccgcca aatcctccaa
     9241 caaggacggc gcatgggcat ttattgagaa ctacctgaat cagccgtttg atgatctata
     9301 ttcctatgga cttcccgcca gaaagtctgc actggatgat atggtggaga aggcactgaa
     9361 tgtgacatat atgacagatg aaaacggtga gcagatcctg gatgagaacg ggaatccgat
     9421 tccggaggac ggtacttccg gcatcagtta cggagactgg gagtacacct atcacactcc
     9481 taccgaggaa gaaatcaata ttctgaagga gctgatcagt gtggctgaac cttccagtgc
     9541 aaccggtaat gatgagatca ccaatatcat cacggaggaa gcggaagcat tcttcaaagg
     9601 tcagaagtcc gtagcggatg tggcaggtgt cattcagagc cgtgtacagg tctatgtgaa
     9661 tgaaaacaga taaaggaaag gcttaaaata tcgtgagcag acagcatgtg aaacatacgc
     9721 gtaagcatca caaattagat agaagaatgc gaaaactcaa gaggagctcg gggcttttta
     9781 tagcccccag cttccttgga gttcttgcgt tcttttttct accctttctg gttgtgatct
     9841 tttattccct ggtggacaat cccatcagta tgaattttgt ctttttagac aactttagaa
     9901 atgtagtgca gaatgccgct ttccgtacag cggtacataa taccgctgtg ttctcggcca
     9961 ttgccgtacc gctggcagtg gtattgtccc tgctgcttgc cattgtattg gaatccaaac
    10021 ttccgttccg gagccagttc cgtacgtttt tcttaagccc catgatggta ccggtagcct
    10081 ccatcgtgtt gatctggcag gtattgttcc attataacgg tgcagtcaat gatcttctgg
    10141 gagtcttcgg cgtagagaag attgactggc tgaagtccag ctattccatt attgttatcg
    10201 taatcctgtt tttgtggaag aatcttggat ataatatgat cctgttcatg gcagccctgg
    10261 ccagcattcc caatgagctg ctggaggtgg cgaggctgga gagcgcaaca ccgctacaga
    10321 ccttttttta catcaagatc cggtatctgt catcgacttt tttgtttgtg acgattatgt
    10381 ccctgatcag ctcttttaag atcttccgtg agatctatct gctgacaaca gattatcctt
    10441 acgattccat ctatatgctg cagcatttca tgaacaataa gttcaagagt ctggattatc
    10501 agacgctgtc tgcagcagcg atactgatgt ctctcgtcat ggtcgtgatc atcggacttc
    10561 tgttcgcagt ggaaaatcat ttcggaaagg atgtggaagg ctgatggagc agaagcttaa
    10621 aaaacctgta ccgcctgaac ttagaagggc aatccggttc aaaaagagaa agaagatatt
    10681 tgagaatgtc cgcatcagca tagcaacagt gatcgcagcc ttttttgcca tactgttcct
    10741 gatgccgatc gttctgacga tcaccaattc tttcatgtca tcttcggaga tatcagcgaa
    10801 ttatggttcg gtatttgcca cgaatgataa gggtggcaag gtctatattt ccgaaaaagt
    10861 gaatttgaaa tttattccgg atattgtatc cttcagccag tatactacgg tacttttgaa
    10921 aagcccggaa tatctgctga aattctggaa ttccgtgatc ctggtgggac cgatcgtact
    10981 gtttcagctg atcgtggcat cgctggcgtc ttatggattc gccaggtact cggggaagct
    11041 gaggtcattg gtattttttt cctatgtgat attgatgctg atgccgtatc aggtaacgct
    11101 ggtacccaat tatctggtgg cggaaaagct gaatatatta aatacgtact gggccatctg
    11161 gctgcccggg atcttttcac cctttgcagt ctatctgctg acgaagttca tgaagagaat
    11221 tcctgtgggg gtcatggagg cggcacagat cgacggtgcc ggagagtggc agatctatcg
    11281 gctgatctgt atgccattgt gcaaaggagc gctgtgttcc gcagccatcc ttgtatttat
    11341 tgattactgg aatatggtgg agcagccgct gatcttgtta tccgatgcgg agatgcatcc
    11401 cttgtccgta ttcttaagca agatcaattc cggagagatc ggactggcat tcgccgtggc
    11461 tacgatctac atggtgccca gtcttctggt atttctctac ggagaagaat atctggtaga
    11521 tggaattacc taccagggcg gtgtcaaagg ctaggatcac tgatttttga ggatataccg
    11581 gcatggatcc gactgccgga atacagatag aaagtgaaat atagaggaga atcaagatga
    11641 acgagaacag tggaaaaaag agaagagaat gggttaaaaa tgcagccatt atattcttgt
    11701 ctgtaatgtt ggtgctgaca tttttttcca atacatttat gaactattcc cttccggagg
    11761 tggcggccca gtacgtgcag tctggaacga tcacggcaaa gatccgcggt accggtacgg
    11821 tggaaagcgg agatccctat aatgtgaaga tcagtgagac ccgtacgatc tccagtgttc
    11881 tggtaaagac cggggacaag gtagagaagg gtgatccact gtttttgttg gaggataagg
    11941 aaagtaagga actgaccgat gcccaggctg cactggataa ggcaatgctg gattttgagc
    12001 tggcattgtt atccggtgac atttccaatt ctaccttcca gaatgtacag aacggaaatg
    12061 tggcttccat aaacacttat cagagtcgca ttgtggcggc ggaggcagag atcgacagat
    12121 ggcagaagca ggtggatgaa gccaccaacg caatcaatca gctgaagacc gcacaggtga
    12181 acgtggatgc cggaggaacg ccggacaccg gttcggagca gaataaggtg aacgcagccc
    12241 aggcagcatt aaacagtgat gaagtcaaga ttgcgaaaga caagatcagc gaatggcagg
    12301 cagctcaggc cacctgtcag gccaccatcg ataaatacaa tgagaacatt gcaagttcgg
    12361 tatccggtaa tggctttgtc aatcaggtga cagaggacga ataccagctg gcactgaaga
    12421 acagggaaca gtatcagtcg ctgatcaatg aacgacaggc atttatcaat aacaatccgg
    12481 acaaggtgaa agcatatgat gagaaggtaa aagcgcttgc agatgccaac aaggcactgg
    12541 cagataaaca gaacagcaag gagaacagca ccaattccct gacggtgcag acccagaact
    12601 ggcagaccga actggataaa cgaaatatcc agctgaaagc agcacaggat accaaggaac
    12661 agctgctgaa ggatatttcc acggaactga atctggatta ccagttagac agtctccaga
    12721 agcagagaga tgatatcgca aaattacagg agaatgcggt aggtgccagc atcgaagccc
    12781 ccatttcggg taccattacc agcgtaacag tcaaggcagg agacgaggca cagccggata
    12841 ctgcactggt gaccatgcag ccggagggta agggctttac catgagcttc tccgtgacca
    12901 atgatcaggc gaagagactt tctgtgggag ataaggcaga cctggtgaac tcctggagat
    12961 acagtgatat ggatattacc ctggccagca tcaagccgga taccacagat cccggacaga
    13021 agaagctgct gacctttgat atcaccggag atgaagtgac acccggacag tccttaaatg
    13081 tatccgtagg acagaagagt gccaattacg atctgatcgt acccaacagt gccatccgtg
    13141 aggacagcaa cggtaagttt atcctgatcg tggaatccaa gagcagtcct ctgggcaatc
    13201 gttatgttgc aacccgtgta gatgtggaag tgttggcaag tgatgacacc cagtccgcag
    13261 tgagtggggc gttatatggc tctgaatttg tcattacgac atctacccag ccggtagagg
    13321 caggaaagct ggtcagactg gcaaataatt cataaaaggt ctttcagacg gaaaataatt
    13381 taacaggaag gaagaaacag atcagatgtt tcggattcga atacagaata aaaagaaatg
    13441 catatggtac tgcagtgcag tagtatcctt tcttctgttc ctgcttttgc tgggatggac
    13501 caaccatctg gcaaataccc aggatgccca gcagatggcc ggacgctgga gtgaaaaaaa
    13561 ggatgtggca cagatcagct gctttttctc ctccaaagcg gaagtgacgg aggataccat
    13621 tcaaagcttt gaatattcac tgaaaaatgt gctgcaggag gcctctatta cagagacctc
    13681 agagaacccc agtgcaagac tctgggtgga tgcctacagt gcggacggca agatcaccct
    13741 ggtcaacggg aaaaatacgc tggatgccaa tgccatcggt atcggcggag acttttttat
    13801 gttccatccg ttgaaactga tcaccggcgc ctatttttcc ggtaatgatc tgatgcagga
    13861 ttattgtatc atcgatcagg atgcggcctg gcagctgttc ggctctaatg atgtggtggg
    13921 catgacagta tatatcggca atgtacccca tatcgtcacc ggtgtggtgc agcgcccgga
    13981 cagcagaatg gataaagcgg cgggactgaa ttccacggtg gtctatgtgt cttatgagac
    14041 actgtctgct tatggtacga ataacgggat caatcattac gagatcgtga tgccgaaccc
    14101 tgtggatgag ttcgcctacg gcaaagtaaa agaagggatc ggcatagatg agaaaaacgt
    14161 tgagatcgtg gaaaacagca gacgttattc tctgcccaac cggatcaaaa caatccttgc
    14221 cttcggaacg cgttccatga acggaaaagc catcatctac ccctactggg agaatctggc
    14281 gagaggctat gaagacatta ttgcattgct tacggtagtt atgctgcttc tgttattata
    14341 tccggtagtg accgttatct ggtgctttgt acactggtgg cgccataagg gctggacctt
    14401 gaaggaggta tggcataccg gtaaggataa ggcagagcgg gctgtggaac gccgcagaga
    14461 gaaaaagcac ccccacaagg agagggatgt cctggaggaa ttggaacggg aactggagga
    14521 agacccttat gcagacagtg aattctcctg gctgacggat gaaccggcgg aggaaacaga
    14581 gcttgcggat gcagcttcgg aagagccgga gaaagcgcag acaatgaaca tgcagacaga
    14641 ggaaacgcag acaccggagg agacaaaaga ggaacagcag tcgtaaaaca acagacacag
    14701 acaagaaaag gagagggaga atgaaaaaga aagggaaaaa actgatcagt atcgcgttga
    14761 ccgtggcaat gatcgcaggt ctgactgcct gtggaaaggg taacggcggg aacggaaacg
    14821 gcggcagcag tgcggccgat gccagtcttg caaaacagta tgtatttaag gaacaggccc
    14881 tggatctcaa tatggattca gacaatatga atattgatct gctgaccaga aagggagata
    14941 cggtttacct tctgtttgag acctatgaat attccgataa cagcagtcag agtacgctgc
    15001 atcttactac aatgaaacag gatggtaccg tcattggcac cgtagaactg cctatgtgga
    15061 aggaaggaga ggctccggct actccggcac ctgcacaggg cggtgaggct tctggggacg
    15121 gaaatgaagc agtgcctctt acggagcagg cggccagcac acaggatatt gcggcagcgg
    15181 atatggatgt ggtagatgca ccggcaggga acagcagtta tactagcaca tcgctcaaca
    15241 attgtacggt cggctcggat ggaaatgtct acggaatacg caattactat agtgagacat
    15301 attccgaaga tgattacagc agtacgaatg attatacatt gatctgctgg agtgcggatg
    15361 ggactatctt atgggaagct gatctgaatg atatgaatac agaagaaagc tattcctggg
    15421 tacatagtat catccccaat gaggatggga ccctgacact gatgttaagc ggtgataaaa
    15481 tagagaaatg cactgtgacg gaagagggta tcacagaccg gaaggagctg ccagaggcag
    15541 cagcaacact tttcaacaat gcaagctcga atatcattac gccggatggc aagctgcaga
    15601 tcatctatta tgatgagagc aattacacgg atatgtatat cgcaacctat gatcctgcaa
    15661 cagatgcggt atcagaagga ataaagcttt cctcgacact gaccaacggc ggttattata
    15721 atatggtgcc cggagacggt gacatcctgt actatgccga tagtaacgga ttgtttggtt
    15781 ataatgtgca gacacaggag tcgaagcaga tcatgagtta tatcaattca gatctggctg
    15841 ccggcagcct gaacaatttc ctgatactgg acgaggaaca gttcctgggc ttttactatg
    15901 ataacaacga aggcaaaata tgcggcggtc tgttcaccca cgtgaagccg gaggatatca
    15961 aggacaggat cgttcttaca ctggccggta actatatcga ctatgatctg aagagaaaag
    16021 tagtagagta taataagtcc ggtgacactt accgtattgt agtcaaggaa tacaatacct
    16081 acaacacttc agaggattat actctcggtg tgaagcagtt aaataatgat atcatctccg
    16141 gaggaatgcc ggatattctg gtcgtggaca gcaatatgtc aatggacagc tatattgcca
    16201 agggactggt ggcaaatgtg gatgatctca ttgcggggga tgaggaatta tcgaaaaacg
    16261 actatctgca gaatgtctgg gatgcgtacc gtgtggacgg taaactgtac tatgtgattc
    16321 cttccttcta tatctctact atggtaggaa aagaatctat tttcggtgac cgtacctcca
    16381 ttactatgga agaactgcag acgatccggg ataccatgcc ggaaggaacc gcactcttca
    16441 gtgatgagac cagagacagc ttcctgtata ccatgatgaa ttattgtggc agtgatttcg
    16501 tggatgtatc caccggaaaa tgtgcatttg atacggataa tttcgtggca atgctgacct
    16561 atgcagccgg gctgccggta gaatatggag aggattactg gggagaggat tactggaata
    16621 actatgaatc ccagttccgt gaggacagaa ccctgctgga tggcatctcc atcagcaaca
    16681 tccgtgatct gaacggtacg atcaacggtg tcttcggtga ggatatctcc tttgtgggct
    16741 tcccgaccga tggagacatg ggatccatat tatgggcagg taacaggatg tatgccctgt
    16801 ctgcaaaatc caagaacctg gacggtgcat gggaattcct gcgctactat ctgacacagg
    16861 aatatcagga taaaatacag gaacaggaat acaatcttcc ggtacttcgc agcacctttg
    16921 agaagaatgt gcaggatgct acgaagaagc cgtattatac ggatgagaac ggcaacaaag
    16981 tggaatacga tgagacctat tacatcaatg gggaggaaat actgctgccg cagctgacac
    17041 aggagcaggt ggacaggatc gtaagctttg tggaaagcgt gaacaagaga ggatactata
    17101 atgaggcaat ctccaatatc atttcggaag aagccggagc atacttctca ggacagaaga
    17161 gtgcgagaga tgtggcaggt gtcattcaga gccgtgtaca ggtctatgta aacgagaaca
    17221 gataaacgga agatagcact gcgtagagca gaatagtaaa agatggcggt ctgttgaaaa
    17281 acaggccgcc atttatagca tggcgcctta gccaaggcgg atgggaatgc cctgcttttt
    17341 cagatgtgtc gtcagggtaa gctcctgtcc gtgctccctt aaccactgct tatagatccg
    17401 gtaatccggc atgatgtgct cgaccatgtt ccagaagtcc ttcgaatggt tcatatgagt
    17461 caggtggcag agctcgtgga ctaccacata atccagagga ccggcgggac cgtagatcag
    17521 gcggtaattg aaggataaag ttcctctcga ggaacagctt ccccagcggg tcttctggtc
    17581 acggatggtg atagaagtgt agtgcccacc ggtgaacctg tggtagtatt ccacacggtt
    17641 cgtgaacact tctctggcag cattccgata tcggttttcc aaagcctgca atctgcggag
    17701 ctccgcaacg gtcagcggct gattgtcata tagattcatg agagtgcctt tcacatacag
    17761 ggatgttgat cctctgagta tcgttgtaag tttgaacaga ttaaatatac tgcgtatggc
    17821 ttgaaaagta aacgcagaaa ttgtagaata ttgcgtaacg gctcgcagac gaaacgaaat
    17881 gtctgtttcg gaaataaggc cggtcacaaa gcggcgcagg cgcattcatg tgactttggg
    17941 aatggcaagg acggaaccgt tacaggaaag gaaaaaagta aatactatgg gtaaatgtat
    18001 tttnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnggacgga accgttacag
    18121 gaaaggaaaa aagtaaatac tatgggtaaa tgtattttga ttggcgcagg agatctcacg
    18181 atgggagaac tgtctgtgac ggaggaagat tatgtgatcg cggtggacgg cggcttaagc
    18241 tactgcgggc tcctgcatgt ggagccggat ctgattatcg gagattgtga ctcgatgacc
    18301 gaacaggaga agcttgctgt ggaacagtta caacagaccg ttccggagaa gatctgccgc
    18361 ctgccggagt gtaaggatga cacagacatg ctggcagcta tcaagcgggg gctggagctg
    18421 ggttataccg atttccgtat ttatgcggcc acaggtggga gatttgatca taccctggcg
    18481 aatatccagt gtctgctgta tctcaaaaat catggtgcgg tggggtacct ggtagacggt
    18541 acgggaatgg tgctggtact gcagaacgaa gccgtgcatt tccggaagga gctggagggc
    18601 accatgtcgc tgtttgccct gacgaaggag gcaaaaggcg tgaatatccg cggcatgaaa
    18661 tattctttgg aaaatgccgt gatcaccaac gatttcccca tcgggatcag caatgaattt
    18721 ctgggagagg aagcggaggt gtccgtagag gacggtgaac tggtatgcat gatccggtac
    18781 tatgaggatg aagtataacc atgtaaactg tgaaaaataa acaggcctga tgcgattttg
    18841 cacaacgaag gtgaagttgt cagaaaataa aaacggagga aaattatgaa aaaatcagta
    18901 agtggagaat ggaaagtaag ggcctgcgga gtgcttctgg cagtaaccat ggcagccgga
    18961 atgaccgcct gcggcagtaa gtccggggat aacggcagca cacagggaac ggtacaggaa
    19021 tccgtacagg cgcagacgga cggagttttt gcggacggca gtgagctggg agagggtaat
    19081 accgagttta ccctgaccgt aacggataag gaaggcactg agagcagttt taccattcat
    19141 acagacaagg aaaatgtagg agatgccctg acccagatcg gcatgatcgc aggggaagag
    19201 ggagattacg gtctctatgt gaagacggta aacggtgtaa cggcagatta tgatgtggac
    19261 cagacctact gggcatttta catcaacggc gaatatgctt ctaccggtgt agatgccact
    19321 tctgtgactg ccggtgatac ctattccttc aaagtggaga aataacagtt gaaaaacgga
    19381 aaactgacga tcagagagat ggcggttttc gcaatgctgg gagcgttaat gtacgcttcg
    19441 aagatgatca tggaggtggc accgaatgtc cacctccttg gtgtttttac gattgcgttt
    19501 accgtggtat atcggaaaaa agcattgtat cccatctata catatgtgct cctgaacggg
    19561 atattctgcg gttttgccac ctggtggatc ccttacctgt atctgtgggc agtgctgtgg
    19621 ggagcgacca tgctgttacc aaaacggatt ccggagaagc ttcgtccgct tgtctatatg
    19681 ctgctgtgtg ctgcccatgg ctttttattc ggaactttgt atgcaccggc gcaggcactg
    19741 ctgtacggtt taagctttca gaaaatggtt gcctggatca tcagtgggct gccctgggat
    19801 ttcgtgcatg gtgtcagtaa ttttttctgt gggatactga tcgtacccat agtaaaaata
    19861 ctaacgtttc tcgaaaaaaa tctttagaaa atcttttgtt ttttctgtag gtgaaattag
    19921 aaattataat ttagaatcag aaactgacag gggagagaat ccccttgccg catgcggcag
    19981 aatgagaggg agtcatggag aacaataaat atcatattct gatcgtggag gatgataagg
    20041 agatccggga tggtgtggag atttacctga aaaatcaggg gtatcaggta tggaaagctg
    20101 ccaacggtaa ggaaggactg gagatcgtag cacaggagga gatccatctg gccattctgg
    20161 atatcatgat gccggtgatg gacggtgtga cgatgttaat gaagctgcgg gagcagaatc
    20221 acgagttccc ggtgatcatg ctgtccgcca agtccgagga agtggataag atcatgggat
    20281 taaatatggg agcggatgac tatgtgacca aaccctttac gccgctggag ctgttggcga
    20341 gggtaaattc ccatctgcgc agatattcca aatatctgac ggcagtcagt ggggaagaac
    20401 aggagaaatc ccatgtgtat accatcggcg gtttggaact aaacgaagaa accgtggaag
    20461 tgacggtgga cggagaagcg gtgaagctga ctcccatgga attcaagatc gtacagcttt
    20521 tgatcaagaa tccgggcaga gtattttccg cagatgagat ctatgagcgt atctggaacg
    20581 agaaagcggt caacaccgat actattatgg tacatgtgcg gaacatccgt gaaaaaatcg
    20641 agatcgatcc gcgaaatccc aagtatctga aagtagtatg gggtgtagga tataagatcg
    20701 acaaacaata aatacggctg tggaaaacaa agcgtttcgg caggcagagg aaaatggccg
    20761 gagaggtgta agaatggaac aggtaacaga ggctgtgaaa gcggagacaa cagaaaacag
    20821 ggaaaaccga ggaaacagaa agcctgcagt caaatggctg atcgtcggac tgattgtcag
    20881 tctggcagtt tccggcattt ttactgcctg ctacggaatc ttcgagcgca gggcggagag
    20941 caggagaaat gaacaataca ttgcggatcc gctggagacg tcgaatacga ttacgtattt
    21001 gtatcagaac tgctatctgc tctataggga tctgtataac aaagaacacc agacttcctt
    21061 aagctatgtg gaattatata tgcagcccac ggagggaaac gaatggctca atgatgaccg
    21121 gaaacgggca gaagtggcat atggagatac ggaggagctc acgataccgg aaggagcaga
    21181 tcccgaaaac tatgcggccc agggaatctt tgcggcgcag accggggaat acctggaaga
    21241 acatttcaat accctggaaa actcctttgg tcagctgaat aatttgtatg attataggat
    21301 acgggatact cagaccggag agatcctaag taatctggcg gatacagata tcgtggcgga
    21361 ggaacagagc ttttatctga cttttgtatt tgatgcctat ggaaatgatg ccaccttagg
    21421 aaatacggtg atcggatcgg atacgacaac ccttcgtaag acagcggcgg aagtgatccg
    21481 taattgcggt cttacgcgaa tggtggatgc caatatgggc acaacgatcg atgccggtca
    21541 taataccatg ttccggctgg aaatgccgaa aaattgtgag atcaccttct gcatctccag
    21601 acaggcggca cagagtctgg gacagaacgg tcagtatatt tattatgatc aggacatgaa
    21661 caatgtcagt gtttactttt ccatgggaga ccagtataca tcctactatc tgtccggatg
    21721 ccagcagatg tttctgatcc tgtgtctgtt gatcttcctg ctggcgcttg tattgccggg
    21781 acttgaggaa gagagagcct gggagaaggt ggttctgctg aagctgcctg tcgaggcgat
    21841 cgttgtgatc cttgtggtga tcgccggcat tggaagtgaa caggtggtag gactggtatc
    21901 ctgggtaagg agcggtcatg cgctgaacac ggttttacag ggaaacagtg cgcagataca
    21961 gatgtcgacc acaatactga cctatgcatt aaatgttctg gcagtggcag ccttgttttt
    22021 cattgcctgg tgcttgggag tatccctgcg ggcaatgcgg gttcagggag ccagagcgta
    22081 tttgagacag cacagcttgt gctatctgtt agtgcctttc tggaaaaaac tgtggagagg
    22141 aatgaagaag ggattctcca ttggcaggga taaggtggtg gatgtctatc atgatgcaga
    22201 gcattttgat gtgacgaagg atgccaaaaa gctgatcctg cggattgtgc tgtggaatgc
    22261 cctgatcctg gtggtgatat gcagtctgcc gctgggagga ctgaccatcg caatcatcta
    22321 ttctgtgata ctgtactttc tgctgcggcg ctatatcagc aaattacaga aaaagtatgg
    22381 tttgttatta aaggccacta atgagatcgc ccagggcaat ctgaatgtga ccattgagga
    22441 ggatctggga gtatttgaac ccttcaagcc gcagatctac cggatcgaag agggctttcg
    22501 taatgcagtg gcggaggaag taaagagcca gcggatgaag tcggaactga tcacgaatgt
    22561 atcccacgat ctgaagaccc cgctgacggc tatcattact tatgtaaagc tgctgcagga
    22621 gccgggagtg acgcaggagc agcgcaagga gtatctggag acgctggatc ggaaagcttt
    22681 gcggctgaaa gcactgattg aagacctgtt tgaagtcagc aaggcaaatt cccggaatat
    22741 cacgcttgat atccgcgatg tggatatcgt aagcatggtc aagcaggtgg aattcgagat
    22801 ggaagacaag ctgacggatg ccggactgga agtgcggatg agcctgccgg aagagaaggt
    22861 catcgtgccg ttggacagtc agaaaacctt ccgtattttt gaaaatctgt ttggcaatat
    22921 tgccaagtat gcgttacccg gcaccagagt ctatgtcaac ggtttcacgg caaaagagga
    22981 tgtgacgatc atcttaaaaa atatcacggc acaggagttg tccgtatccg gagaggaact
    23041 gacggaacgg ttcgtcagag gagacacctc acgtaatacc gagggcagcg gactggggct
    23101 ggccatcgcc aaaagcttca cggaactgca gagcggcaaa ttccggatcg aactggacgg
    23161 agatctgttc aaggtggtat tgacctggaa agtaaagtca cagaatggtc aaaatatggt
    23221 tcccagagag tctgagacgg cagaaaagaa cgaaaacagt gactgtttat cataaaatct
    23281 gttgcatgat aataataaaa taaatcaatg ttttaatact aaaacatcca catttacgta
    23341 gaaatatgtt aaatgtggat gttttcaatt acaaatgagt gtgctacaat aatgaaaact
    23401 tcaaagagaa gtattacgga tctgaagtgt ggaacagctg tgcagaacgc acaagctgta
    23461 tgaagagaaa aaggagcagt aaacaatggg aatgacaatg acacagaaga tacttgcccg
    23521 tgcagcgggt cttgagcagg tacaggcagg acagctgatc gaggcacagt tggatctggt
    23581 gctgggcaac gatatcacca gtccggtggc aatcaaggaa atggataaaa tgaaggtgca
    23641 gggggtgttt gataaggata agatcgcact ggtaccggat cattttgttc ccaataagga
    23701 tattaagtct gcggagcact gcaaatgtgt ccgtgagttt gcaagacgca atgagatcac
    23761 caattatttc gaagtgggag agatgggtat cgagcatgcc ctgcttccgg agaaaggact
    23821 tactgtagcc ggagatgtga tcatcggtgc agattcccat acctgtactt acggtgcttt
    23881 gggagcattt tccaccggtg tgggaagcac ggatatggca gccggtatgg ctaccggtaa
    23941 ggcatggttc aaagtacctg cagcaatcaa attcaatctg atcggcaaac ccgcagaatg
    24001 ggtcagtggt aaggatgtga tcttacatat catcggcatg atcggtgtgg acggtgcact
    24061 gtacaagtct atggaatttg taggggacgg tatcgccaat ctgtccatgg atgaccgttt
    24121 caccattgcc aatatggcca ttgaggccgg tggcaagaat ggtattttcc ctgtggatga
    24181 gaaagctatt gcttatatgg aagaacactc caagcgtccc tataaagtat tcgaagcaga
    24241 cccggatgca cagtatgatg cggagtatac cattgactta agcactttac ggcctaccgt
    24301 ggccttccct catctgccgg agaatacaca taccatcgat gagatccacg agatggacgc
    24361 cattgccatc gaccaggtag tcatcggttc ctgcaccaac ggacgtattg acgatctgcg
    24421 caccgcagca aaggtactca agggcagaca tgtggcgaaa ggcatgcgct gcatcgtaat
    24481 tcctgccacc cagtccatct atatgcaggc catggaagag ggacttctga agacctttat
    24541 tgaagcggga gcagtcgtaa gcactcctac ctgtggaccc tgccttggtg gatacatggg
    24601 tatcctggca gaaggcgaga gatgtgtctc cacgaccaat cgtaactttg tcggacgtat
    24661 gggacatgta aaatccgaga tctatctggc aagtcccgca gtagcggcag ccagcgccat
    24721 caccggcaag atatcctgtc cctgcgaact ggagtaagct gcaggaaaaa gcaagatgcg
    24781 ggcaggcaag taagggattg tggaatcctg tggctgcctg catggaaaag taagagatat
    24841 attggaacag gaaaaatatt tgaaacattt gtaatagaaa ggtaacggaa aatgaacaac
    24901 gcaaaaggaa tagttttcaa attcggagat aacgtggata ctgacgttat tattcccgca
    24961 agatatctga attcctccga tcctgcagaa ctggccactc actgcatgga ggatattgac
    25021 aaggacttta ttaaaaatgt aaaaaagggt gacatcatcg tagcggagaa aaacttcggc
    25081 tgcggctcat ccagagagca tgcccccatc gccatcaagg ctgcaggtgt cagctgtgtc
    25141 atcgcagaga ccttcgccag aatcttttac cgcaatgcca ttaacatcgg actgcccatc
    25201 attgagtgtc ccgaggcatc caggaagatt caaaacggcg atgaagtcga gatagatttc
    25261 aataccggag tgatccaaga tcttaccaca ggcgaaagct tccagggaca ggcgttcccc
    25321 gagttcatgc agaagattat cgccgcagag ggactgatca actacatcaa cagtcaggaa
    25381 taaattgagt gcatggctcg taacgtaggg ttgccataac ccccacgaac gcacgcagca
    25441 gatggaacat ccttccgggg tgtcaggtct cccggaggga atttcctgac gaaaagtgac
    25501 atagcggtcc gaaaatatca ccatcgtccg acaaacattc ccggaaggac attccatccg
    25561 ctgcaggggt tctacatttt ctcataaaat ctgtacgtca taggcatttg ggcctacagc
    25621 catttctatt gttttggtgc aaatcgcatc aaaatagcaa aatcagtaca aaaaattttc
    25681 taaatcaaac attttttgta ctgattttat tatttttccc attttagcga aaataagctc
    25741 ttataaaagg ccaaaatctc ctgtttttgt attttttatg caaaaaaagg cgtttttaat
    25801 acaaacttta ttgtaccact gccttatcca catcaatcac tgcgtagaaa tccgggtggt
    25861 tcatctggat ccgtttcgtg acggagtcaa ccacatcctg atcggaaacg ggataatcat
    25921 aggggactac gatgtcaaaa atgaggttcg tgtgggtcag tcccttgacg atgcggaaat
    25981 catgcatgct gagttttgga tcgatctgat ccagataagc ttttacctct gctttcaggg
    26041 acagtgtttc ggggtcattg gtgctgacgg gatccatgtg gatcacagcg ttacagttta
    26101 atttgctgcg cagttcatgc tctatggtat cgatcgtatc gtgcagggtc agaatattac
    26161 cgtcggcagg aacctccgca tgcagagaga tcagagtcct tccggggccg taattatgca
    26221 cgatcagatc gtgaatcccg gtgacatcat cataagagag aacgatgtcg ttaatctgct
    26281 gtaccagttc aggctcgggg ggctgcccaa gcaggggact gatcgtgtct tttgctgcga
    26341 caaatccgga gtacaggatg aacataccca ccagcacacc acagtaaccg tcgaaaatga
    26401 tgcccgaggc ggcggagacg atagtgccga tgagaaccac ggtggttgct atcatatcgc
    26461 tcaaactgtc aatggcggta gccttcattg ccacagaacc gatccgggaa ccgatgctgc
    26521 ggttatacag agccatatag cactttacca ggatcgaaaa taccaggatg ccgataatga
    26581 caggggagta gtcaggaact tccggatgga aaatcttact tacagagctt tttaccagtt
    26641 cgaagcccat gagcacgatt aaaatagaga ctaacagccc ggagatatat tcgatccgtc
    26701 cgtgtccgaa gggatggtca gagtccggct tct
//
LOCUS       DAWDHD010000017        26702 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_146_length_26702_cov_11.218036, whole genome shotgun sequence.
ACCESSION   DAWDHD010000017 DAWDHD010000000
VERSION     DAWDHD010000017.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 26702)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 26702)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..26702
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146_length_26702_cov_11.218036"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         7606 a         5445 c         7225 g         6426 t
ORIGIN      
        1 tatcgtatgc tccgggagtt cgtggatttt gccaatgtat ttaactaact ttccatttac
       61 agtgtaatat tatccacctt tttccattcg aaaaaaatcc ccctttttga aaatttgcta
      121 tctgttgcct gatggaaact aatggtagaa ttcaattatc aaataaaaca gacggccgtc
      181 agtgataatg ataaatacaa aaaacaatat ttacctattg ggaggacgag tgtatgaagc
      241 gtaaattatt atctaccgta cttgcggtaa ccatggcagt atccaggctg accggatgtg
      301 gacagagcgg aacaaatgaa gcaacaggaa caggcgctgc aacgacagat gccaaaacag
      361 agacctatcg tgtccggatt cctatagaca gctgcgattt ttccctgacg cattacgaag
      421 cagacgggga agaggcggat gagaattttg aacacttttc ttttgaaaga gtggaaaaat
      481 acattctgcc actgctggat gcggcggaga aagcttacgt cgggaagctg gatatcctcc
      541 tgtcccccct gaagtccgcc ggtctacatg aagaccaatg gagagcggaa caacggcggc
      601 aaattaaagc cggaataccg gaagcgctgg gcagagtata tctgccgtta cattgaggaa
      661 taccgcagcc gcgggcacct gtggcatttt acacatttcc taaaagcggg agcacagcgg
      721 atcggtgtta ccagatacac agataagatc gaggtgacgg cgtttgaaaa ggacgggcgg
      781 atcacggtgg tgcttttaaa ccggacggag gaagagattc cggtgtatct gcggctggga
      841 gagtactgtg cggaactgac atcaaaggca aagtccatta tgacggcgga gatcgaaaaa
      901 taagaaaatc gatataggca attatgaaaa agacagcggg aaccgggagc ggaacccact
      961 gtttttgcgt atacgtccgg ggagcaggtt gacagaacgg tggaaaccgt agtattttga
     1021 aacagaccac tgtggataac agggcaagtg aaaaatgtag gcagaagaaa agtaagttac
     1081 gacagaagaa tgaggataca gatatatgaa aatctatttg gtaagacacg gggagacaga
     1141 ctggaaccag gcggggcggc tgcagggaca gacggatatt gacctggatg ctcaaggttt
     1201 tgcgcaggcg gcggaagcgg cagagagatt aaaggaagtt ccttttgaaa tcgcgttttg
     1261 ttccccgctg atccgtgcaa gacatacggc agagaccatt gtgggcgaga ggaaaattac
     1321 gctgaccaca gacgaaagac tccgggaact caatttcgga ccatgggaag ggaccgatgt
     1381 ccgtaaaatc cgggaggggg ccaacagtcc gtttacggat ccggggagct atctcccgcc
     1441 cgagggagcg gaaagttttg cccagttgta tgccagaagc ggcgagttcg tgaagcaggt
     1501 gttgctgccg ttggagggaa cttatgaaac agtgctggtg gtagctcatg gagcattgaa
     1561 tcgcagtatc ctgaatgcgg tgttggatat tccggtcagc gacttctggc atttccatat
     1621 gggcaactgt gctactgcga ttctggaatg ccgggaagga aagctgtctc tgttggaata
     1681 tcaggacagt gaaagtgtaa atacgaagag caaactgctg taagttccgg atacagagct
     1741 tgcagactgc tgaaaggtga gaggttaaga atgcacatag acagagaaga catgctggaa
     1801 ctgaccagaa gaatgaccgt gtcccgcaac tgtttttcca gaattgcagg agcctatatg
     1861 gacgaagaag ggtttattga cggtactttt aatactcatt ttctgaagtt gtccggaagc
     1921 gaaaaagaga aaaatctgaa tattgccaag acggtacctt attccaatac caatgtgaat
     1981 ctgaaaaatt accggctgga tcagggagcc cggaaaccgg gcagcattat gcagttattg
     2041 gaggcattac ggcagtgcga actgaaaaat gatgcattat tgtactccct ctatgaatat
     2101 attggggaga gataccagcc gggatatcct tatgccgttt atcttttctt cggcagctac
     2161 gatgtaccca ccaagggaaa agacaaagag aaccagtggg agtccgagga agtctatcag
     2221 tttctcatcg gcacgatcag tccggtgacc ggagactacg aggccggaaa accggaggcg
     2281 ggatttttgt accctgcctt tgccaaccac agcacagaca ttgacggtat caatatctat
     2341 caggcggagg aaggggcaca gttatttgca aaaatactgg gcgtacaggc ttgacagaaa
     2401 ggcatttctt ccctataata cgctctggaa caaaatgaat aaccggtcat aaaacccgta
     2461 agggagtgga tttttcaaga tatcgatctt attttcgcca cacagcagga atgttgtgtg
     2521 gcgtttgttg tttaaggaga ggaaaaatga accagactta tatgaaagaa aagaagatat
     2581 tcccgttggt catgtccatg gcacttccca tgatgctgtc catggcattt aattccctct
     2641 ataacattgt agacagttat ttcgtggcga agctcagtga agatgccatg acggcattgt
     2701 ctttagtcta tccggcacag aacttgttga cggcggtggc agtgggcttc ggtgtgggtg
     2761 tgaatgccat gatctccttc tatctgggag cacagaatca ggaaaaagcg gataagacgg
     2821 caacccaggg catggtgctg ggagtattgc acggcgtact gcttatgatc ttattcctct
     2881 gcggcatgaa ctggttctta gggatgttta cgaagagcga taccgtgcgg gcactgggta
     2941 tggaatattc caatgtggta ttcctgtttt ccaccattgt caccggaggt atcacgttag
     3001 agaaaatctt tcaggcggtg ggccggatgc gtgccaccat ggtcagtatg atcgccggat
     3061 ttgtgacgaa tattgtgttg gatccgttgt tgatcttcgg tatcggaccg tttccccgga
     3121 tggagatcgc cggagcggct ttggctaccg gaatcggtca ggtgatcacg ttattagtat
     3181 atatcatcta ttatgtgttg gatccgttgc cggtaaaatt gcgcagaact tatctgcgac
     3241 cggaaggaga gatctgtggc aagacctacg gcatcggcat tccggctacg ttgaatatgg
     3301 cactgccgtc tctgttgatt tccgcactga acggtattct ggcggcttat tcgggtgcgt
     3361 atgtgctggt actgggcgtt tattacaagc tgcagacctt tatctatctg ccctccaatg
     3421 gcatcattca gggcatccgg ccgctgattg gttacaatta cggtgccgga gagcggaagc
     3481 gtgtggagca gatctaccgg ttgacgctgg aactgactgc cgggatcatg gctatcggca
     3541 cggcattgtg ctggctgatc cccggaggac tgatcggact gttcaccgag aatccggaga
     3601 ctgttaccat cggtattaca gcactgcaca tcatcagtct gggctttatt ctgtccgctg
     3661 tgtcggtgac cagctccggc gcactggagg cgctgggaca gggcatggct tccctggtga
     3721 tctccgtgat gcggtatgtg gcggtgatca ttcctgcggc atttgtgctg agccgcctga
     3781 tcggcgtcac cggtgtgtgg tgggcatttg tctgcacgga gagcgtgact gcggtggtgg
     3841 cgtatgtgtt gtaccacagg gtgtggaaga gagcgtccgc cgcccggacc gctcagatcc
     3901 ccggccggtc accccgcgcc attcgtaccc gcggccgcgc acccagcgcc atacacattc
     3961 gcggccgttc accccgcgcc attcgcaaaa tcgcctcttc gaggctttcc gctgctacgc
     4021 agctgccttt tgctcatgag atggcgctcc cctcgtcgat ttgcacagag ccggctcctt
     4081 acgcaagcgt aaagccgtct ccgcacaaat cgacgggtga actgatgtat acacaatatc
     4141 ttgtatcctt taattgactt cccctacaaa gtattgtaga atagaccgtg ataggtttta
     4201 cgggcgaaag cccatgggga cacgaaggta agggggtaca taagatgttt caggtaatca
     4261 aacgagatgg ttccaaatcg gactttacac tgacgaagat caatgatgcc attatgaagg
     4321 cttttactgc gacgcagatg agctacaata atgacattat tgatcttctt gcgttacggg
     4381 taactgcgga tttccagaaa aaggtggaga atgacgagat ccacgtggaa gacattcagg
     4441 acagtgtgga gcgtgtactg ggacaggccg gctatgaaga ggtggcgaag gcgtatattc
     4501 tgtaccgcaa gcagcgggag aaaatgcgtg ccatgaagtc taccattctg gactacaagg
     4561 atgtggtcaa cagttatgtc aaggtggaag actggcgtgt gaaagagaac tccacggtga
     4621 cctattccgt aggtggtctg atcttaagca actcgggtgc ggtgacggcg aactactggc
     4681 tgtctgaaat ctatgatgaa gagattgcag aggcacatcg gaatgcggat atccatatcc
     4741 atgatctgtc catgctgacc ggctactgtg ccggatggtc cttaaagcag ctgatcaagg
     4801 aaggactggg cggcatcacc ggcaagatta cttctgctcc ggcgagacac ttatccgtgc
     4861 tatgcaacca gatggtaaat ttcctgggga tcatgcagaa cgaatgggcg ggagcacagg
     4921 cattttcttc ctttgatacc tatcttgccc catttgtaaa ggtagataat ttaagctatc
     4981 ccgaagtgaa aaaatgcatc gaagcattta tttacggtgt caacaccccc agccgctggg
     5041 gcacgcaggc tcctttttcc aatatcacgc tagactggac ggtgcccgat gatctggcag
     5101 agctgccggc gctggtaggc ggtgtggaga tggatttcaa atataaggat tgtaaaaagg
     5161 aaatggatat ggtcaataag gcattcatcg agaccatgat cgagggcgat tccaacggcc
     5221 gcggtttcca gtatccgatt ccgacttatt ccatcacgaa ggattttgac tggtccgata
     5281 ccgagaacaa cagactgtta ttcgagatga ccgcaaaata cggcaccccg tatttttcca
     5341 actatatcaa ttccgacatg cagcccagcg atgtccgcag tatgtgctgc cgattgcgcc
     5401 tggatttaag agaactgcgt aagaagaccg gaggattctt cggttccggc gagagcaccg
     5461 gaagcgtggg cgttgtcacc atcaatatgc ccagaatcgc atatctgtct gccaacaagg
     5521 acgagtttta cgcaagactg aaccacatga tggacattgc agccagatcc ctgaagatca
     5581 agcgcggtgt cattacgaag ctgctgaacg agggactgta tccctatacc aagcggtatc
     5641 tgggaacctt tgagaatcat ttttccacca tcggtctgat cggtatgaat gaagtaggct
     5701 taaatgccaa ctggctgaga gcggatatga gcgatcccag gacccaggag ttcaccaagg
     5761 aagtactgaa ccatatgaga gagcgtctgt cggattatca ggagcagtac ggagacctct
     5821 acaatctgga ggcaacccct gcggagagta ccacgtaccg tctggcaaag cacgacagaa
     5881 agcgctggcc cggcatcaag acggccggaa agcccggaga taccccttat tatacgaatt
     5941 cctcccatct gccggtggac tataccgtgg atattttcga tgcactggat attcaggatg
     6001 aattgcagac cctgtatacc tccggcacag tgttccatgc attcctggga gaaaagcttc
     6061 ccgactggaa ggcagcggct tctctggtac gcaccatagc ttccaattac aagctgccct
     6121 attacaccct gtctccgact tattccatct gtaaggagca tggttatctg gcgggcgaag
     6181 tgaaggtatg tcctcactgt ggggcgaaga ccgaggtcta cagccgtatc accggttact
     6241 atcgtccggt acagaactgg aatgatggta agctacagga atacgccaac agaacagagt
     6301 atgatattgc acattcttcc ctgaagcgtc ccaccagaag cgtggtgacc ttgtccaatt
     6361 ttgcagaaga ggtggatgta aaagtggagc agccgcagaa catcaagtat ctgttcacca
     6421 ccaagacctg tcctaactgt aagctggtga aggaatatct gaaaaatgta ccctacgtga
     6481 ccatcgatgc ggaggagaat atggagctgg caagacgtta cggtgtaatg caggcaccga
     6541 ctctggtggt ggtaaacggt gacagccata aaaagtatgt aaatgcttca aatatcaaga
     6601 agtacgttga ccaactgaca ttagttgggg tagaatagaa acaggtcagg aggcatcgcc
     6661 cgaaggcggt gcctttgctg tgtaataata taaattcaaa agcagatcta ttgcagaacg
     6721 gaggaaatat gatcaacaaa ctgatcactc gaatcaaaga aaccaacgca ccgatcgtgg
     6781 taggactgga tcctatgatg aaattcgtgc cggaatatat taagaaagca gcattcaccg
     6841 aatacggtga gacactggaa ggtgctgcag aggccatctg gcagtacaat aaggggattg
     6901 tggatgctat ttatgattta gtacccgctg tgaagcctca ggtggcaatg tatgagcagt
     6961 tcggtattcc cggcatggtg gcatttaaga agactgtgga ttattgtaag gaaaaaggcc
     7021 tgatcgtcat cggcgatatc aagcgcggcg atataggatc cacttccgaa gcatatgcgg
     7081 taggacatct gggcaaagta caggtaggca gcaagagcta ttacggtttt gatgaagatt
     7141 ttgtaaccgt gaacccttat ctgggatccg acggtgtaaa tccttttgtg aagatctgta
     7201 aggaagagaa aaaaggtatt ttcgtactgg tgaagacttc caatcccagc agcggcgagt
     7261 tccaggatca gctggtgaac ggcagacctc tgtatgagct ggtaggagaa aaggtagcag
     7321 cctggggagc tgactgtatg ggtgattcct acagctatgt aggtgctgtc gtaggagcaa
     7381 cctatcctga gcagggcaaa gtactgcgta agataatgcc caaggcattt attctggtgc
     7441 ccggttacgg tgcacagggc ggtaagggcg cagatctggt acattttttc aatgaggaca
     7501 gattgggtgc cattatcaac tcttcccgtg gcatcattgc cgcataccag caggagaaat
     7561 acgcacagtt tggcgagcag aattatgcag atgcttcccg tgcggcagta ctggatatgc
     7621 gtgaagatat cgcacaggcg ctggcagcac gctaggcaga aaacagctac tattcagagt
     7681 ctcattcgca aaatcgtctc tttgagactt tacttttggc tctgaatagt gataaatgta
     7741 tggggtgtcc acaagcggac agatgggaga acagaggatg aaagaagagt tatatattac
     7801 cttccactcg gaggatgata tcagacattt tgtagatacc tgcaacgaat tcgacgatgc
     7861 aatcgacatc cgtattcaga agatggcaat ggatgcaaag tcacttttgg gaatgctcct
     7921 gatgaagact gaggaaccgc tggagatcga atatgcctgc tatgatgatg aagacaatta
     7981 cgaagaattc aagcaggcca tcctggaaaa atacgacgta aaagtcgtac aggctacgga
     8041 aaagcaggca gcagataaaa ttgtttgaaa aaattgaaaa attctattga cattttcgcc
     8101 cgcagcagtt acaatacata ctgttgcagg cgaaaacctg taaatgaaat gtacgtgtag
     8161 ctcagctgga tagagcgtct ggctacggac cagaaggtcg tgggttcgaa tcctgtcacg
     8221 tacatctaag gccccggaga tgatgtctcc ggggttttta ttttgcattt tagaaaagtt
     8281 ttagaaatac ttcccgatcc gtatatttac gtggtactta tgtcatgcta aaatgatgat
     8341 gtaaatggag gaagtcgtga aagtatgaac aaacaggaat ttgtggaccg actgcggatg
     8401 gcgctgaatg ggcgtgtttc tcccggtctg gtcatggata atgtaaatta ttaccaggat
     8461 tatataaata cagagatccg caagggcagg acagaagagg aagtcttaga gagcctggga
     8521 gatcccagac tgatcgcccg cacaatcatt cagaccaacg gtggagacaa ccgttccgga
     8581 taccggaaca cggtctacgg caacggagag tatcgggagg ctgccggaca gaatgtccat
     8641 cgtagttata cagaggaaga cgatggctac ggaacacacc agagtaaggt gggaaatgtt
     8701 ctggcgaagc ttccgggctg ggtgtggctg atccttggga ttctggtggt agtattgatc
     8761 ttcggtgcgg tattttccgt attgtctttt ctggcaccgg tactgattcc gattgtggta
     8821 gtattgttcc tggtgaaatt atttcgggac tggcttaact agcaactata taaatcagct
     8881 attcatagtc cctcaattcg caaaacgcct ctgcgaggct tactttttgc tcattgaggt
     8941 tacttcgtca aataaatata atcggagtac cttacagatg cgagcatctg acggttgctc
     9001 cgattatatt tattgactct gaatagttcc aacgtaaaga gccgccaggg ggaagctggt
     9061 ggctctcatg aggggagtat aaataaataa tacataaggg tgcaaataga atcatgaagg
     9121 ggtttctatt tacaattatg attatagtac taatgatata aaaatgcaag aagaaattta
     9181 gttctaaagt accataattt ttgggcatat ttttgtgcga aatagacata ctactcctgt
     9241 aactaaaaaa gatatttcct atcaggaggt cacaaatatg tctaacaatt atgaacagaa
     9301 caatcagaac aagtataaca actcttatga cgagagcgag aacagtaaga actcccagaa
     9361 taagcagaac agccagaatc agaataagaa tcagggcaac aacagggagc agaacaagag
     9421 ctgctaatcg ggtgcataat ttttcagggg ctgccgcaag gcagccctat ttacatattt
     9481 tcagaatcca tatataataa ctttatatgt gcaataattg atgactaaga tccggaggaa
     9541 cgaatgagac gttttgaatt gattgctccc tgccatttcg gtatggaatc agtattaaaa
     9601 agagagatca cagatctggg atatgatatt acagaagtgg cggatggtcg tgtcaccttt
     9661 ttcggagatg aggaggcttt gtgcagagca aatatttttc tgcggacggc agaaagaatc
     9721 cttatcaaaa tcggaagttt tcatgcggag acttttgaag aactgtttca gggcacgaaa
     9781 aatcttccct gggaagaata cattccgaag gacggcaaat tctgggtggc caaggcagct
     9841 agtgtaaagt ccaaattgtt cagcccctcg gatattcagt ccattatgaa aaaagcaatg
     9901 gtggagcggc tgaaggccca gtatgagatc agctggttcc ctgaagatgg agaaaatttc
     9961 cctgtccggg tattcctgat gaaggatgaa gtgaccgtgg gactggacag caccggagag
    10021 tccttacata agagaggata tcgtaagctg acggcaaaag cccccattgc ggagaatctg
    10081 gctgctgcac tgatcgaact gactccctgg aatgcgggaa ggatcctggt ggatcccttc
    10141 tgcggaagcg gcaccttccc tattgaggca gccatgatgg cggccaatat ggcacccggc
    10201 aggaaccgca gttttacggc ggaagaatgg ccccatatca taggaaaaaa ggtatggtat
    10261 gatacaatgg atgaggcgga agagatgatc aatctgtcag tggagacaga tattcagggc
    10321 tatgatattg acgaggatat ggtgaagatc gccagagaaa atgcacggat ggcgggtgtg
    10381 gacaagctga ttcatttcca gcgtcgtggt gtggaagagc ttcatcaccc gaagaaatac
    10441 ggctttatta tcaccaatcc tccctatgga gagcgtttgc aggataagag ccagatgccg
    10501 cagttgtacc gtaccatcgg agaacgattc cgggaactgg attcctggag tatgtatctg
    10561 atcactgcct atgagcaggc agagaaggat atcggcagga aagcggacaa gaaccgcaag
    10621 atctacaacg gaatgatgaa gacttattat tatcagtttc tgggaccgaa gcctccgaag
    10681 agaagagacg tttagaagaa atgaaaaatt tactgcagat cagggatcaa caacagaata
    10741 gacgcattac attgatcctc tggctcctgc tgggaagtat gtctatgggc atcatgctct
    10801 ggacggcagc gcataagacg gtggtgatct ccgcgttaag tcaggaacag ggcggtctgg
    10861 tcacagagaa tcaggcggaa cggtcccatg aaatgcagct ggacatggcg gaagacagga
    10921 aggcagaacg ggagatctgt ataccattgg agaccggtac gaaagcggaa aatgtagtgg
    10981 tggaaaatca ttacatggaa agggaactct ggatctatgt gcagaacggc agaaaatctt
    11041 tttaccggga acatcagctt accggagatt tcagcctggt gggaaacggc atctgtgaag
    11101 cacagaacga gggcgtattg ctcagattgt ccatgaagga gatcctggag tatcacagta
    11161 ctctggaaga gggaactctg aagatcgatt ttgtcaatcc cagagaatct tacgaccgga
    11221 tcgtagtgct ggatcccgtg ggaggcggaa gggacagagg cgttgccgat tccggatgtg
    11281 aggagaagaa catcgctctg gaggtggcaa gacagacggc acagctgttg gaaggaagca
    11341 tggtaaaaat atatcttacc cgtacagaag atacagaggt cgcacaggaa gtaaggcgta
    11401 gcttcgctga ctgggtggat gcagatctgt acctggagat cggactgtca gcagatgatg
    11461 cacaggagag tacctacggc atccgggcag aatacaatga tgagtattat ctgccggatt
    11521 tcggaaatgt acaatgggcc gactgcgtga ccagacaagt gacggtggct tccagcaacc
    11581 gggccattgg attgttcccg gcggaggaag atgacattct gtggcagctg aagattccgg
    11641 cagccagaat ttctctcgga tatgtgacca acagcagcga gagacagctg ctggaacagg
    11701 aaagctatca aaaaaagcta gcggaaggta ttgcagaagc tatttccgag gtttatacta
    11761 atacgcaatg atgactatat aagaacacaa atgcggcaag gagttaatat gcagggaaga
    11821 atcgtattcg cgaccggcaa tgccggaaaa atcaaagaaa tccgcatgat catggaggat
    11881 accggtctgg aagtcgtatc catgaaagat gcgggcatta aagcagatat tgaagaaaac
    11941 ggtaagacct acgaagagaa tgcactgatc aaggcccgtg cggtggctgc ttttacgaaa
    12001 gatatcgtga tggcggatga ctccggactg gagatcgatg ccctgaataa ggaacctgga
    12061 atctattctg ccagatatct gggagaggat acgccgtatt ccataaaaaa tgcaaatctg
    12121 atccagagac tggagggagt gccggaggaa aagcgtactg ccagattcgt ctgtgccatt
    12181 gcagcagtgc tcccggacgg cagagaactt accacaagag ctaccatcga agggcggatc
    12241 ggatatgaag agaaggggac caacggattc ggttacgatc ctatttttta cgtaccggaa
    12301 ttcggcaaga ctacggcgga gttgacagaa gaagagaaga accaggtcag tcacagaggt
    12361 aaggcattgg agctgatgaa agaggaactg aagaaatatg aagatactga tcgtaagtga
    12421 tacccataaa aagaatgaga attatttcaa agtactggag atgcaccacc cggacatggt
    12481 gatccactgc ggagatgcgg agggcagtga gtatgcactt tcttctgcgg cagaatgccc
    12541 ggtttatatc gttctgggca acaatgactt tttctcggat ctccccagag agatcacact
    12601 ggatatcgga tcctacaaag tatgggtgac ccacggacac aattattatg tatccatggg
    12661 aaatgagact atcaaaaaag aggccattga ccggggcgtt gatatcgtgc tgtacggaca
    12721 tacccatcgg ccggtgatcg atattgatga tgacatcatt gccgtgaacc cgggaagtct
    12781 gtcctatccg agacaggagg gacgccagcc ttcctatgcg atcatggaga ttgacagaag
    12841 gggaaaggtg cattttacga ttgcttatct tcgccagtaa tgcttcaaac ttctctcttg
    12901 acagatagaa aatttctggg ttataataac atctgcgttg cggaaatccg cgataacgca
    12961 gcttcggggt gtggctcagc ttggctagag cacctggttt gggaccaggg ggtcgcaggt
    13021 tcgaatcctg tcaccccgac gtgtaacttg gattttgtat ttatttttca aaaaattaat
    13081 acaaaactgt tgacaaatga tgtcggataa gttacaatat gcttcgtcgg ttcgctgatg
    13141 tagataacag acagatatgt gttcgtagct cagctggata gagcaacggc cttctaagcc
    13201 gtgggtcggg ggttcgaatc ccttcgagca catttgagat aaggaagact tttggtcatc
    13261 actgtctcgc atggtgggta tagcgcagtt ggttagcgcg ccagattgtg gctctggagg
    13321 ccaagggttc gaatcccttt atccaccttt attatgagtg aagtgtcgta atggcacgct
    13381 tggtgtcata tttatagggc tatcgccaag cggtaaggca cagcactttg actgctgcat
    13441 tcgctggttc gaatccagct agccctgttt cattatgggg tattagctca gtcggtagag
    13501 cacttgactt ttaatcaagt tgtcgggggt tcgaatcccc catgcctcac taggtgttgt
    13561 gatatcctga tttggggatt cgaaccccct ggtcgagatc ccccatgcct cacttggttc
    13621 ttttatacag catcctcaca ttttgaaaac ccctttcggg gcttttttta ttccattttt
    13681 atagaagaaa ggaaatacta tgcagactaa agaaaacaag atgggtgtca tgcccataga
    13741 caaattactg atctccatgt cactgcccat gatgatctcc atgctggtac aggcactgta
    13801 caatattgtg gacagtattt tcgtgtccag gatcaacgaa tatgccctac gtgctgtatc
    13861 tctggcattc cccatccaga gtctgatgat cgctgtggcg gtgggaacag cggtaggtat
    13921 caatgccttc ttgtccagaa ccctgggaga aaaggatttt gagaaagcca atattattgc
    13981 cagaaacggt atttttatcg caattgccag ctatataggc tttgcggtga tcggtgcact
    14041 gatcagcaga ccgttttttt ccagccagac ggaagttatg gaggtgcggg aatacggtgt
    14101 gacctatctg acgatctgtt gtgtggcggg aatgggtatt tttctgcaga cgacttttga
    14161 gaggctgtta caggctaccg gtaagaccat atatacgatg attacccagt caaccggcgc
    14221 gatcatcaat atccttctgg acccgatcct gatctttggg tatttcggta tgccccgcat
    14281 gggaatcgcc ggtgctgcag tggctaccgt gtttggacag atcgttgccg catccatggc
    14341 attatggttt aatctgaaat tcaataagga actggatatt tccatgaaag gattccatcc
    14401 caacggtcat ctgatcggac agatttataa ggtgggagcc ccttccatcg tggtacaggc
    14461 tatcggttcc ctgatgacct atggaatgaa cctgatcctg gcggcattcg gttccgccca
    14521 gacagtgttc ggcatttact ttaagctgca gagctttgtg tttatgccgg tgtttggact
    14581 gaataacggc atggtgccta tcattgccta caattatggc gcaaggcata aggaccgggt
    14641 tattaggacg attaagcata gtgtggctta cggtgtgggt atcatgtgcg tgggactgat
    14701 tgtaatgcac atcttcccgg cacagctcat gcgtatgttt gatgcggagg aaagcctgat
    14761 cgccatcggt gttccggcgc tggagaccat ttccctaagc tttgtgtttg ctggcttctg
    14821 tatcgtggtg ggaagcgtat tccaggcact gggtaacggc atctacagta tgatcgtgtc
    14881 cgtggccaga cagatgtgcg tgctgcttcc ggtggcaaag cttttgtcct taagcggtga
    14941 agtgacattg atctggtggg cctttcccat cgcggaactg gccagcctgc tcttaagtac
    15001 ctatttcctc gtaaggatct ataaaaagat catatgccac attggagaac cggtaacgac
    15061 aagagagatt acagaataaa atagaaacag atgcggcggg gatgcttgac aagcaaaagg
    15121 aaggaagagt ataattattc aaggtccttt ggcagagagc attcccgttt gtcaaggttt
    15181 taagctaatc gtcagatatg gcagttagta agcggatatg gcggaagtgg cagacgcgat
    15241 agatttaggt tctattgtca acagacgtgc aggttcaagt cctgttatcc gcattgcgta
    15301 attatggaag catagctcag ccgggatgag cgttcgcctc acacgcgaaa ggtcaggggt
    15361 tcgagtcccc ttgcttccac tcgaaataac tattcagagc tcatgacaag gactgaatag
    15421 ttattttttt aatcagagag catcggataa gtaatgtacg aaagcacaga aatataaagg
    15481 aatacaaaag ctatgaagaa aaatgtaaga aaacagggaa ttctgctgcc gatatccagt
    15541 attccctcga catatggtat cggaactttc ggtaaggaaa gttaccgttt tgtggacttc
    15601 ctggaaaaat caggaaacca tctctggcag atcctgccgt taggccctac cggctacggc
    15661 gattctccgt accagtcctt ttccaccttt gcggggaacc cctactatat tgacctggag
    15721 cttctgatcg aagacggcta tctgacaaaa gagatctgtg acagctatga tttcggagac
    15781 aatgagcatt atgtggacta tgagaagatt tatctgtccc gttttaaagt cctgaaaatt
    15841 gcgtttgaca atgccaggga acagggcctt acagagaccg gagactatca gaaattcctt
    15901 acggaaaatg cgtactggct ggatgactac gctctgtata tggcggtgaa aaattctttt
    15961 ggcggtgtct gctttatgga atgggacgag gacatccgtc tcagaaaagc agcggcactt
    16021 cgcaaatacc gtaagaagct ggcggaagaa atcgcatttt attgcttcca acagtacctg
    16081 tttgcaaaac agtggaaaga actgaaagcc tatgccaata agaaaggcat tgggatcgta
    16141 ggggatattc ccatctatgt ggcatttgac agtgccgata cctgggcaaa cccgaagctg
    16201 ttccagctgg ataaggaagg ccggcccatc ggagtggcgg gatgtccacc cgattgtttc
    16261 tccgagaccg gacagttatg gggtaatccg ctctacgact ggagttatca taagaagacc
    16321 ggttatgcgt ggtggatgca gcggatcgcc tattgctatc agctgtatga tgtgcttcgt
    16381 attgatcatt tccgcggctt tgatgaatac tggttcgttc cttacggaga tccgacggcg
    16441 gagaacggcc attgggaaaa aggccccggt tacgaattat tcgatgtcat gaagaagaaa
    16501 ctgggcagga agaaagtgat cgcggaagat ctgggatttc tgactcccag cgtgatccgg
    16561 ctggtgaaga agaccgggta tcccggaatg aagattcttc agtttgcttt tgatgccgga
    16621 aatgacagtg aatatctgcc gcacaattat gacaagaact gtgtggtcta taccggaacc
    16681 cacgataacg atactacggt ggggttctta cagaatatgc cggagaagga caagaaattt
    16741 gcaaagaaat atctgggaca taagaatgac aagaagcttt gctttgagat catccgggca
    16801 gcattgtcca gctgtgcgga taccgcaatc attcccatgc aggattatct ggagctggac
    16861 agcagcgcca ggatcaacac gccctccact ctgggatgca actggaaatg gcgtatggat
    16921 aaggatgctt tggatccgaa gcttgcgaaa aagatttata agatggcaaa attatacgga
    16981 agactgtaaa gtgtgccgtg tagcgtaagg cacattgtaa gaatacggaa gggatagttt
    17041 cctttccgta ttttttgtgg acaaatcata aatatttgca tgcatgaaaa tggggataac
    17101 tatggcaggt agcatttttt agtgcatttt tgtcaaaaca agaggatttt tttgtggtct
    17161 tatggaaaaa gccacgttat tatggtatgg ttatctatat tgggcggagt ctgcaatcgg
    17221 atttctgcga gagagaggga aaacatgagc cgtactactg acagagcaag tcgtaataag
    17281 ggacaatgga tcgtcattgg gtttatggct tttgctatca tagtcatttc gatatgcaca
    17341 gtaatctttc gttcctatgt gcggggattt cataaagtaa cagacagaga aaaatatgac
    17401 cggtattatg tgatgatcac agaagaccgc aaatcggatt tctggcagtc cgtataccgc
    17461 ggagcttttg aacgcggaca ggaagagaat gtatatgtgg atctgctggg cgagcatctg
    17521 tctcaggagt atacgagggc agagcttatg cggatcgcca tgtcctccgg tgtagacggt
    17581 atttttgtag aatcggatga aagcgacgag atgtcacaga tgatcgacga agcagtagaa
    17641 cggggcatcc ctgtggtgac gctgtatggt gacaatacgc acagtgcacg ctgcagcttt
    17701 gtcggtgtag gaagctataa tctgggcagg gaatacggac gacaggtatt gaagctggct
    17761 tctgtcagtg agagtaccgc acccatgacg gtagcggtac tggtgaatgc ccatgcactg
    17821 aactcggcac aaaatattgt ctgctccggg attcaggacg cactggaaca ggagaaaatc
    17881 cggggaccgg agatagaact ttccttaatt acagtggacg atacgaatac tttttccgtg
    17941 gaggaatcca tccgggatat ttttatgaat gaaaaactgc cggatatcat tatctgcctg
    18001 aacgaactga gcactacctg tgtctaccag gcggtagtag attacaactg cgtgggcgag
    18061 gtcgcaattt tagggtatta tgataccgat acgatactca aagcaatcga gcgaaatgta
    18121 gtcaatgcaa cgatctcgat agacacggag cagatgggag ctttctgtgt ggatgctttg
    18181 caggagtact atcgttttgg ctataccagc cagtatttta cggcggatgt cacaattatt
    18241 gatcagaata acgtgggtga atatttacaa gcgcaggaag cgacagagtg aggtacgcat
    18301 gaggaaaaaa tttcgacagc tgtctcttca ggcgaagatg agcagcattt ttgtattggc
    18361 gaatctggta gtatttgcag tgacggtgat attgcttctg ggaatcaaca gcatgtcttc
    18421 ggagatcgat aaggtctatc aggggaatct gcggctgaac gaagtatctg atgcgctgat
    18481 ggcggtgcag gacagtatga cggattatct gaattccaag accagtgatt ccctggagga
    18541 attttaccgg aatgaacaga catattccca actggtgcag gatatgagcg atgaggtgac
    18601 gggagccacc tttcagagaa tggaacgcag tatccggcat atgtcggaag aatacctgga
    18661 cctcgtaagt cagacgattg aggcaaagcg tgggagaaat gtagagaaat accgcgtccg
    18721 gtacgagaac gcgacacaga tgtatgaata tatcaatacc tatatttaca gtctgaacaa
    18781 cgaacagttc cgcagcaact ccgagaacta cagccgtctg tcggctgctt tccgtaattt
    18841 cgaaatggtc agtgtgattg cattatccat cgtcattgtg gtcagcgtat ctatcgtagt
    18901 aaagcttacc ggggagatca tcagtccgtt aaaggatctg gcggtaaccg ctgacgaagt
    18961 agcgggcgga aactttgata ccgaactgtt acaagtacag tcggaggatg agatcggcgt
    19021 ggtaacggga gcatttaacc agatgcttat cagtatcaga cagtatctgg aaagcctgcg
    19081 cgagagcatg gaaatccggc gttccttaat ggaaaaagag cttttgatgg aagcacactt
    19141 aaaggatgca cagctaaagt atctgcaggc gcagatcaat ccccacttct tattcaatac
    19201 gttaaatgcg ggagcacagc tggccatgat ggaaggcgcc gaccgtacct atgaatatgt
    19261 acagaatgtg gcggaatttt tccgctataa tgtaaaaaag agtaatgata tcgtgacggt
    19321 cagagaagag ctggaactgg tggatcatta catttatatt ctgaatgtcc gcttttccgg
    19381 ggatatccat tatgggaaag aaattgagga ggatctgctg gacagctgca tgccaagcat
    19441 gatcttacag ccgatcgtgg aaaattccgt aaatcacggg atcagagaga tgaacggaga
    19501 gggaaagatc cagctgaagg tataccatgt ggatggagat acgctgggga tcagtatccg
    19561 ggataacggc atcggaatgg accgggatac gatagaaaaa gtacttggcg gcaattatca
    19621 cgaagagcag tcaggcggcg gttccaatgg tattgcaatg gggaacatta tcgcaagatt
    19681 aaaacttttc acgcaaaacg aagatgtaat gacgatttta agtgacggga aaaatcaagg
    19741 aacggaagta atcctgtatc ttccgataaa agagagggaa aagacggatg tataagatca
    19801 tgctggcgga tgacgaagga atcgtgatcg attccctgaa attcatcatc gaaaaagaat
    19861 tcaaggatac ctgtgaggtg cagtatgcca agaccggcag aagtgtgatt gaactggcag
    19921 agagcttccg tccggacgtt gcggtcatgg atatccagat gccgggaatc aacggcatcg
    19981 atgctatgaa agagatccgg agaagcaata atcatacagt gtttatcgta atgtcagctt
    20041 atgacaaatt tgattatgcg aaagaagcta tcaaactggg agtcatggag tacattacca
    20101 agccgatgga gaagacccgg atcattgcag cattgcagaa ggcaatggag cgaatcgatg
    20161 cagagcgcct gaaacgcaaa aatgagttgt tgattaagga aaaactggag acagtcgtac
    20221 cgattattga aagcggtctg atccacaata tcttattaca ggagcatttc cgcgaggata
    20281 ttgagaatta ccggtcaatt ttaggaatta cgctgcaata tgcctacatg atcgctgtga
    20341 tatgcggaga tgaacagcag ggaaaccaca tgacgaatgc ggtgggaagc agtgtcagaa
    20401 tgcagaagca ttatcaggag gtcagggact gtctgaaaga aaattttgac tgtattgtgg
    20461 gtaccgtcat ggcaaacaaa ctggcagtgc tggtacctta tgagaaagat gtgatggact
    20521 ataatgagcg tatcgaactc attgaaaaag ccagagaact caccagatat ctgcgcaaga
    20581 gaacggatat cagcttccgc atcggcatcg gagaaccgaa agattttctg atggctgcgg
    20641 aatcttatac ggaggcgctg aatgcactgg tggcgaccac cggcagtgtg gcacatgtgg
    20701 atgacctgcc gatcaaatgc gaatacggtg gaaattatcc ggtgaagctg gagaaaaaac
    20761 tttttgcgga agtcgaggac ggtgacattg acaatgcttc cgcatcggca tcggcatttt
    20821 ttgactggat ggtggagagc ggttccgatc tgatgaatat gaggcttaag gtgctggaat
    20881 ttgtcctgtg gtcagagcac attgcctatg aaaaaggcgg tatgatctat cagctcaatt
    20941 cccgtgcgga gtatctgccg caggtgatgc aaatggcgga accggcggcc atcaaaacat
    21001 ggttcctgga aaaagtgaag gaagcctgcc ggaacgttct gaataaaaga gaagaaaaga
    21061 cgggcagtat tatcgaaaca gcaaagaatt atattaagaa taactatcac aaagatattt
    21121 ccctggatga tgtatccaga gaggtaaata tcagcccgta ttatttcagt aaactattca
    21181 aagaaactac cggggaaaac tttatcgagt acctgaccaa tttaaggatg gacaaggcaa
    21241 aggaactact gcagacgacg gagtgctcca tgaaggaaat ctgtgccaaa accggatatt
    21301 cggatcccaa ctatttcagc cgcagtttta agaaaaatgt gggtgtgacg ccgacagagt
    21361 ataaggaaaa ccggggaaag ggaaaggaac agggcagtgt ataagaaaaa gagacggata
    21421 tgtgttgcgt ggcttctggg gatgatgtta ttgctttgtg cctgcggaaa tctggaaacc
    21481 ggaaaagatc agggagaagt cgcggaaaat gataaaattc agatcggcat gagctttgat
    21541 tcttttgtca ttgaaagatg gcagagggat cgtgacattt tcgtctcaac ggcaaaggag
    21601 ttgggagcgg aagtaaatgt ccagaatgcg aacggtgatc tggagcagca gaaaaagcag
    21661 atcaattact tcatcgacaa aggaatggac gtgatcgttg tgatatgcat tgactccaaa
    21721 ggattaacag aggaagtgca gagggcgaaa gatgccggca ttaagatcat tgcttatgac
    21781 agactgctgc agaatacaga tattgacctg tatatatcgt ttgacaacga aagagtaggt
    21841 accatgatgg gagaggccct ggtggaaaac ggtctggccg gcggaaatgt gctgatgctg
    21901 ggaggctctg ccgtggacag caacgttgct atggtggaaa agggctttcg caaggtcatg
    21961 gaggacaatc aggtgacgat cttagattct atgcatgcgg atggctggaa agcagaactg
    22021 gcggggacgt atatctatga tcatatggat attgtctcgg aagccgatgc cattatgtgc
    22081 ggcaatgatg atcttgcaag caaagtggta catgccctga aagaaaagag actggcagga
    22141 gatattatgg tagtggggca ggatgccgac ctggaagcgt gccagcgcat tgtcgagggg
    22201 acacaggtca tgacggtcta caaaccggtg gaaaaaatgt cgcagaaggc agcggagtgt
    22261 gcggtgcttc tggcaaaggg agagccgttg ccggaaggag atacggtaac catagagaat
    22321 ggaaaatatg aagttccgta cattggtctg gaaccgatca gtgtggataa aaacaatatg
    22381 aatgatgtga tcatcggaag cggatttcac ttaaaagagg atgtttatct gaacattccc
    22441 tcggaaatgc ccaaataata ttggatttac tttgaaaatg gggaaaagag atagattcta
    22501 aaaacacaat aggaaaaatt tggtgacaag gctgtgccgg cagacaagat attgctagca
    22561 cagccttgat tttgtcagga gagaggatgg aaaagtgcag aaggtcgtaa aatcatgcta
    22621 taggatttgt gatattctct atttgtcaac aaaaacaaac caaataaggg aggttacttt
    22681 ttatgaggaa gaaattatta agcacacttc tggcaacagc catggctgtt acaatgcttg
    22741 caggatgtgg atccaatggg ggacagcagg caaccacacc ggcaacagac aacaaaacag
    22801 aaacatctgc agaagcaaag acagatgctt ccactactac cacaacaggc ggcggtaaga
    22861 tcggtgtagc aatgccgacc aaggatctgc agagatggaa ccaggatggt accaacatgg
    22921 aagcccagct gaaggaagcc ggctacgaag ttgatctgca gtacgcatct aacgatattc
    22981 ccacacaggt atcccagatc gagaatatga tcaactccgg ctgcgaactt ctggtcatcg
    23041 catccatcga cggtgactct cttggaaccg ttctggagca ggcaaaggaa aaagacattc
    23101 cggttatcgc atatgaccgt ttgatcatga actccgatgc agttacttat tacgcaacct
    23161 tcgataacta catggtaggt acgaaacagg gtgaatacat cagagatcag ttggatctgg
    23221 acaatgcagc aggacctttc aatatcgagc tggtaaccgg tgatcccggt gacaacaacg
    23281 caagattctt cttcggcggt gccatggatg tgctgcagcc ctacattgac gcaggtaagc
    23341 tggtagtaaa atccggacag accgatttcg aaaccgttgc aaccgctaac tggtctactg
    23401 agaccgcaca gtccagaatg gacgctatca tttccgctaa ctatgcagac ggaaccaagc
    23461 tggatgcagt gctgtgctcc aacgactcta ccgcacttgg tgttaccaac tctctggaag
    23521 ctaactacac cggcgagtgg cccatcatta ccggtcagga ctgcgataag cccaacgtta
    23581 agaacatgat ctccggtaag cagtccatgt ccatcttcaa ggatacccgt actctggcat
    23641 ccaaggtagt agagatggta gacgcagtca tgaagggcgg cgaagcacct gtcaacgata
    23701 ccacaaccta tgacaatggt accggtgtca ttccttctta cctgtgcgag cccgtattcg
    23761 ctgatgcaag caactataaa gagctgctca tcgattccgg ttactacaca gaagcagatt
    23821 tacagtaatt ttataaaata atatggatcc tgcgggatcc ttacggcagg cgggcgcttc
    23881 ccgcctgctg tattcatggg aaagcaaaat agaacggttt gattagataa tgtggaatta
    23941 agacttggga ggaataccat tggcaaaaat cttattggaa atgaaaaata taaccaaaac
    24001 attccccggt gtaaaagcac ttgacaatgt aaacctgcag gtggaagagg gagagatcca
    24061 cgcacttgta ggcgaaaacg gcgcaggaaa gtccacattg atgaatgtat tgagcggtat
    24121 ttatccgtat gggtcttacg aaggggacat catctatgac ggtgaggtct gcaaatttga
    24181 taagatcaag gacagtgagg aaaagggtat cgtaatcatt catcaggagc ttgcactgat
    24241 tccctatatg acgatcgggg aaaatatgtt tctgggcaat gaacggggac ataagtacaa
    24301 aatcaactgg aatgaaacca atgccagggc ggcagaactt ttaaagaccg taggcctgaa
    24361 ggaaggcccc cagacattga taaaggatat tggcgtagga aaacagcagc tggtagagat
    24421 tgcgaaggca ctggccaaga acgcaaaact tctgatcctg gatgagccta ctgcctctct
    24481 gaatgagacg gattccaagg cactgttgga tctgatgctg gaactcaaga aaaagggaat
    24541 gacatccatc atcatctctc ataagctgaa tgagatttct tatgtggcag ataagatcac
    24601 cgtcattcgt gacggatcta ccatcgaaac gcttgtgaag ggaaaagatg atattaccga
    24661 gagccggatc atcaaaggaa tggtaggaag agagatcgcc gaccgattcc cgaagagaga
    24721 gccgaagatc ggagaggtcc tgatggaggt caaaaactgg accgtgcatc atccactgta
    24781 ttctgaccgt aaggtggtgg ataatgtcaa tcttaatgtg aaaaaaggtg aagtagtggg
    24841 aatttccggc ctgatgggtg cgggacgaac ggaactggcc atgagcctgt tcggcaaaag
    24901 ttatggttcc aacatcagcg gcgaactgta tatcaacggc aaaaaagtac agctgaatac
    24961 tgtacaggaa gccatcaaaa atcatctggc atatgtgacg gaggatcgaa agggcaacgg
    25021 actgattctg agcaacccga tcaagattaa tacaactctg gcaaatctgg atgccatcag
    25081 cagacatact gttattgata aagacaaaga gtacaatgtg gcggtagact ataagaacaa
    25141 actgaatacg aaatgtccaa ctgtggagca gaatgtagga aacttaagcg gcggtaatca
    25201 acagaaggta cttttggcaa aatggatgtt cacagatccg gatatcctga tcctagatga
    25261 gccgacgaga ggtattgatg ttggtgcaaa atacgaaatc tactgcatca ttaaccaact
    25321 ggtggcagaa ggaaaatcgg ttatcatgat ctcctcggag ctgcctgaga ttcttggcat
    25381 gtgcgaccgg atctatgtaa tgaacgaagg aaaaatagta ggagaactgg cgggcagtga
    25441 ggcaacacag gagcttatca tgtccaagat attatcagcc ggtaagaaag gagcattggc
    25501 atgaataaaa caaagggcct cgattttata aaaagatata ccatgatcct cgtactgatc
    25561 gtggttacgg cattttttgc atggagaacc ggaggagcaa tcctgcttcc catgaatgtc
    25621 agcagtctga tctcgcagaa cgcatatgtg tttattctgg caaccggtat gctgttatgt
    25681 atcctgaccg gcggtaacat cgatctgtcc gttggatctg ttgtatgttt cgtcggtgcc
    25741 atcggagcgg tactcatggt caacatgaaa atgaacatgt ggctggctat tctgatcatg
    25801 tttgttatcg gtacactgat cggtgctttc cagggtttct ggatcgctta tgtgagaatc
    25861 ccgcctttca tcgtgacatt ggccggcatg ctggcttttc gaggattaag caacgtagtc
    25921 ctgcagggta tgaccgtatc cattaaggac tgtactacat tcgtaaatct gttcggtggc
    25981 ggtgctgact gttatattcc cgatttcttc ggtaatggtc ccatcaatct gacctgtgta
    26041 tttgccggtg tgatcgctgc agtgatcttt gcagtgttac agctgaagaa tagagccgac
    26101 aaggtgaaaa aaggatacga agctcccagc atgggttcca cgatcggcaa aatcgtagtg
    26161 attgctgcag tgattctggc attcagctat cgtctggcac agcacaaagg tattcccagt
    26221 gtattgatct gggtactgat tgttatcatg gtatacggtt acatcacttc caacactact
    26281 gtaggacgtt atttctacgc agtgggcggt aatgagaagg ccaccaaatt atccggtatc
    26341 aataccaaca aggtttattt cctggcttat atgaacatgg gattcctggc agcgttagct
    26401 ggtatggtaa ccattgcacg tatgacttcc gcacagccga catacggaca gaactatgaa
    26461 atggatgcca tcggttcctg cttcatcggc ggtgcttccg catacggcgg aaccggaact
    26521 gtacccggag ttatcgtagg tgctgtactg atgggtgtta ttaacctggg tatgtctctg
    26581 atgggcgtcg atcagaatat gcagaaggta gtcaagggtc tggtacttct ggcagcagtt
    26641 atcttcgatg ttgtatccaa acgtggtgga aaattaattg ccaagaatta atttctacat
    26701 at
//
LOCUS       DAWDHD010000018        26546 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_147_length_26546_cov_11.063569, whole genome shotgun sequence.
ACCESSION   DAWDHD010000018 DAWDHD010000000
VERSION     DAWDHD010000018.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 26546)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 26546)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..26546
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147_length_26546_cov_11.063569"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         6855 a         5646 c         7345 g         6700 t
ORIGIN      
        1 tcaacagata acttctaatt atcccttata atccatccgc actatatata aataagtata
       61 ttataaaatc atacaatttc aagaaaatta catttcgaaa taccgaagga acacagcggt
      121 taaaacgaag gagtatacga aggagtataa atcaaaaagt atgtaagatt ctgtcgactt
      181 ttgtcatgta ttatatagag acataaataa atggcaaagg cggtaaaact atatgtacaa
      241 taccaaagaa aaatgcaaag agcttgcgga aaaattcaaa cagatctgtg ctgaaagagg
      301 cacaacgcct tataagatcg ctcagaaatc cgggctatct tcctctacgg tcagttgttt
      361 tcaggcggct acaacggtac cgagagtaga taccctgatg atactttgca accaacttgg
      421 tatttcggtc agtgatttct ttggagaacg ggaagtgtcg cagacacaga ctgaggatga
      481 agaaaatatc ctgaaaatgt atcgtaatct gccggaggac aggaaaatgg ctttacggac
      541 ttacctgaaa atgctgaatc agtatcagga agaataggga caaacgcagc attctgtggt
      601 atgatgtgag agtaacaggc gatatgtgtc agaaaatatg acacaagaga cctgaaagag
      661 agaacatcga aaggtatcgt acgattatga atactatgac acaggattct tccaccggtc
      721 tttggaacaa taaaaacaga aaaattttct tctggagcat cctcgcgctt gccgtgatcc
      781 tgcgttgcat ccgttttgcc acagtgccgg acggtatcaa ccaggacgag gccatgggag
      841 cggtggatgc ctgggcatta tcaaaatacg gaaccgacag atatggtact tttctgccgg
      901 ttcattttac tgcctggaga tacagtcaga tgagcgttct ccttgcttat tgcatggtcc
      961 cctttatcaa actgttcgga ttcaacacgg taacggtgcg tctgccgatg gtactgatct
     1021 cctccggcgc ggtggtattg gtatatcttg tgggcaggaa attattttct gaacggatcg
     1081 ccctggtggc aatgctgctg accgctgtga atccctggca gtttatgcag agccgctggt
     1141 cgctggattg caatctcttc ccgcatattt tcctgctggg attttattta ctgcttcttg
     1201 gattggaaaa acgacgctat ttatacttgt ccatggtctt tttcggactg acattttact
     1261 gttacggtat cgctgcctat tccgtaactg catttctttt tgtattcttc atatggtgcc
     1321 tgtggaagcg ccggctgaaa ttccgggaag gattgctgtg cgcactgatc tttacggtag
     1381 tggccctgcc ggaagtcata gttctgggaa tcaacgcggt acattttggc agaaccataa
     1441 gtactccgct ttttaccatg ccgtattttc cggacagtgt gagaagcgca gatattctgt
     1501 ttttgaactt ttcctttgca cagttgggga aaaatgcctg ggctctgttc agccatgtat
     1561 tcctgcagct gccggatatt ttcttcaatt cgattccggc ttttggcccg ttgtatcatg
     1621 tgtcgatccc gtttgtcttt gtggggatca ttgtatttac catacagctc ttccgggaga
     1681 aaaatattga aaaacagaca cagatgctgg cattatgggg cttcctggtc accgggatct
     1741 gggtcggcct gatcacttac gaagtcaaca ttaaccgtgt gaatattatt ttctatccga
     1801 ttatcctgtt atgtgcctac ggaatcggtc tggcggttcg gaagtggaaa aagctgtggc
     1861 cggtggtcgc agctgcctat ggaatcagca gtattctgtt cttcgggact tattttacta
     1921 cctatgcgga agaaagcaga gagtactata acaaagattt catggaggcg gtggcggaag
     1981 cggattctct ggaggaatac gaaagtttgt acatcaccgg caatctgggc tggcagttca
     2041 accgggatgc cacggagatc ctgacccagt atgtctgtaa gatagacgca cagtattatc
     2101 aggggaagag taatgtctcc aacggaagag agcttccggc ttatgcggac cggtatcact
     2161 acatctatcc agagcaacag gccgcagaac tggtggagat ggtgggagac ggcctgctgg
     2221 tattgtatca gggagatctg caatacattg atttttccta tgatgtagtg gatacggtag
     2281 gagattatct gctgcttacc gtacagaatt aagagaggac gcttatgaat atcatacttt
     2341 tatcgggcgg ttccggacag cggctttggc ccctgtccaa cgagatccgt tccaagcagt
     2401 ttatcaaaat attccatacg gaagacggcg gcctggagtc catggtgcag cgagtatacc
     2461 ggcagatccg ggaggcggat ccggaggcaa agatcacgat tgccacatcc aaggcacaga
     2521 tctcttccat ccacaaccag ctgggagaaa atgtcggtat cagcatggag ccctgcagaa
     2581 gagacacctt cccggcaatt gcactggcgg tggcatatct gcaggatgtg cagggagtga
     2641 caggagaaga gccggtgatc gtatgtccgg tggatccgta cgtggaaaag gattatttcg
     2701 aagctttgca gcggctggag gagcgtgcgg cacagagtgc ggcccacctg gtgctgatgg
     2761 gtatggaacc gacgtatccc agcgaaaaat atggctatat cattccgaaa acagcggaga
     2821 atatcagtcc ggtggaaatg tttaaagaga aaccggacaa ggagcaggca gcggaatata
     2881 tcagacaggg agcgctgtgg aacggcggag tgtttgcctt ccgcctgaat tatgtattgc
     2941 agaaagctca tgaactcata gagtttaccg attatgaaga tttgctggca aaatatgaaa
     3001 ccctacagaa gatcagtttc gactatgcgg tggtggagaa ggaacctgag atcgaggtca
     3061 tgcgttttgc cggaacctgg aaggatctgg gtacctggaa caccctgacg gaagccatgg
     3121 acagtgcatg cgtcggggag gcggtgctca atgagacctg ccggaatgtc catgtggtga
     3181 atgagctgga tatgccggtg ctgtgcatgg gattgcagga tattgtcgtg gcggccagcc
     3241 ccgaggggat tctggtatcg gacaaagagc agtccagcta tatcaaacca tatgtgagca
     3301 gttttaccca gcaggtcatg ttcgcagaaa aatcctgggg aagcttccgg atcatggatg
     3361 tggaaaagga aagtctgacg atcaaagtga cattgaatcc cggacataag atgaattatc
     3421 acagccatga attccgggat gaggtatgga cggtgatcta cggagaaggc cgggcagtga
     3481 tagatggtga ggaacgcaga gtaaaggccg gtgatgtgct gcggatgccg gcgggatgcc
     3541 ggcatatgct tctggcagac acggagctcc aggtgatcga agtacagctg ggaaaagata
     3601 tcagtgtaca ggataagaaa aaatatcctc ctctaaagcc tttataattg ctgtgccaaa
     3661 caggaaacgt ccctcttcgt tgggatgaac ggcatccacc agatagcagg cagcagtttg
     3721 gggattccat tgcaatgcct cgttggtatt gagaaagaaa acgttcatgg ccgccgcggt
     3781 ttcttcggca ccggtcacat aatcgttcag aatttcatcc agatcattgt tgaaagcggt
     3841 accgttgtcg aacgctgtaa tgaaattcga ggaagcgatc agaatttcag catccgggta
     3901 agcatcctgc agagattgca cccctgcttg cagaccgtca cggtattgct ccacagtata
     3961 tcccgtaaaa tagtcattca atccgtaatt gattacgaaa caaatttttt tctccccgga
     4021 gattttggca gtatcttccg ataaaaagcg ctgtacggca ctggggaagg atttgtcatc
     4081 gggggtcaat tgtgtggcgg aggaacctcc gatggcacgg ttgtgaacgg aggctttcgt
     4141 cagcgcaccg ataacgccgg ggatggaggc aaaatcctga tagtttccga atacgctgtc
     4201 accgaaaaag acgatgtcat aatcggacag atcggagtag gaagcagggg cattaatggc
     4261 tgcctgcacc agggtatcaa actgtgtcag actggagtta tcggcatcta taccatggta
     4321 ttgcaaggtg ccgctcaata ccagcagcat gacggagcgg gcagcgacag catttaattc
     4381 cagaggagtg tcataggcat caggattgga gatcagccat tcttctccgc cggggaagaa
     4441 cagagagaca ttttcccgga cagacagggg agctgccaga acctgcacca gattttcgta
     4501 ggtttcggtg tcactgctgt gggcctgcca gtatttcaga gaaggacagg agaatactat
     4561 ggtaaattca acaccggaat aagaatcgac atatgccaaa agatcctcat cgaaagaggc
     4621 ataaaatcga ctgttattat ggaaattgct gttccacagc aggaaggggt ccaaaatgag
     4681 caatacgtga gtgacctcat ttccggagga aaaagcagtt tccagataat cggaaatatc
     4741 ggaaaaggaa gtcaggcgat gggaacagct gacagcatcg taacccatat aggtagagta
     4801 aatgtcttcc gggaaagctt ccggcgcata catggagcag aagaccccct gataggattc
     4861 ctgctgcagg cgcccaagat cggcggaacg ttttccgggt tcatatattt tcacatagag
     4921 aattccggcg gctgttacgg caagcagcag aactacaata acgataccgg aaaagagttt
     4981 ccatgatttt attttctttt tcgtatttga actcataagg cgaagagatc ctttctgtga
     5041 acagtaagta tggatttata acggttttat tataccgcag caaagcgatg gatgccaaca
     5101 ccattgttgt ttttcttcct tcagtatgat aaaattagta agttgagttg acaaatggaa
     5161 gtatttttaa acatagatgc cgaaaaggat cagacaggag gatatttatg gaagaattag
     5221 ctgtttttgg agggaaaccg gtccgtgaga agcctatatt ctacggacga cagtatattg
     5281 atcaggaaga tgtcgaggcg gtgaccaaag tattgactgc agatgtgatc accagcggac
     5341 cgcaggtcag cgatctggag aagcacttgt gtgagatcac acatgcaaaa tatgcagtgg
     5401 cagtatgcaa cggaacggca gccctgcatc tggcggctct ggccgctggt tttggcgaag
     5461 gggatgaggt tatcgtaagc tccattacat ttgcggcttc ttccaactgt gtattatatg
     5521 ccggtgcaaa gccggtcttt gcggatatta acccggagac ttataatatt gatccggcat
     5581 ccattcggaa gctgatcacc cccaggacca aggcggttgt cgcagtggat ttcaccggac
     5641 aggctgtgga actggatgag atcagagaga tctgcaagga gcataacctg ctcctgatcg
     5701 aggatgcagc ccatgcgatt gggacaacct ataagggaaa gccggtggga agtctggcgg
     5761 atatgacctg ctttagtttt catccggtga aaacggtgac tgcgggagag ggtggtgcta
     5821 ttactaccaa tgatgaaaag ctgtatcgcc atttgctgcg tctgcgtacg catggcatta
     5881 ccagagatcc ggaagagatg gtacatccta ccgacgcacg ctggtataac gagcaggtgg
     5941 aattgggctt taactatcga atgactgaat ttcaggctgc attgctgttg agtcagctga
     6001 agaagctgac acggttctct gaacgcagaa aacagatccg caaacgctac aacgaagcct
     6061 tcgcagacat gccggaactg attccacaga aggagatccc ggagtctgac acgacctggc
     6121 atctgtatgt actgcatctg aatctggaga atctgaactg cgacagacgg cagttctttg
     6181 acgcgctgta tgcagagaat acctgcccac aggtacatta tctgccggta tactggcact
     6241 cttattatga aaaactgggt tatgaaaaag ggctgtgccc gaatgcggag aaatactaca
     6301 acgaagtcat gagtattcct ctgtactacg gattgaccga cgaagacgtg gaagatgtga
     6361 tccatgcggt gaaaaaagtg gtagcatatt acacgaagct gaaataggat ccgactgtga
     6421 aacctggacg gatagcggga aaaggcggca tgagacagaa aacagcagaa aacagtacgg
     6481 gaaaaagaaa taatgtcgaa aatttcatag agatcgttgt gtttgccgta ctggtgggga
     6541 ttgcttcggc ggttactctc tggctgtttt accggcagtg cgtggaaagt atgttgggga
     6601 cgggactgta tcattccgat atgaaggcat acattctgga aatgcaggga ctggacagcg
     6661 gttacagctt tccctatccg atcctcttca agctggcggc agtgatccat ctggtcacag
     6721 gcagtctgcc caccggaacg gaactggcca tggcgctggc aacgatgttg ctgaacagcg
     6781 tagcgatgat cgccctgaaa ataatgctgg acaggcacgt gggggcggag ttgcgtaaag
     6841 ccatgcccgg gaaagcctgg cttcccggcg tactcacagg aacggtagcg gtatccctgt
     6901 ttttcgtatc catggtatat ccgccaacgg gaatctatct gccggggatc aagtacaagt
     6961 atctgggagt atttacggca aatcctttcc ataatgctac ttatatggcg gcacgaccct
     7021 ttgcaatcct ggcatttttc aagtatgcag agctgatgcc tctgtatgaa caggacaatg
     7081 cccacaagga gtatggaaga gattatatcc tcttttctgt ttatctgtta ctggcaacga
     7141 tggcgaagcc gagctttacg atcgtactgg tgggggccgc gggaattctg atgctgtgga
     7201 gaatgttcca cagcaaattc cggaacttca tgcccaccat ttggctggga gtatgcttcc
     7261 ttcctacttt tgcggatctg ttgtaccagt tccgcggtgt gttcgtcccg caggagggac
     7321 aggaaggcgg catcggtttt acgctgggac atgtgtggct gcagtattgc agtaatcttc
     7381 cgctggctat cggtctggct gtcggtttcc cgatcctggt gcttctgttg aactataagg
     7441 agttgtgcag agacagcatt taccgcttct cctggcagat ttacggtatg agttttctga
     7501 tggcgttttg cctgtacgag aagggattcc gggagatgga ttttaacttt tcctggggat
     7561 atatgtacgg tatctttttt gcatttgtgg gagcactgtt ggtattgctg cgggctaccg
     7621 gaaaggcgga tacgaagaag aaaaagggac tggctgcgat acaatggctg gcctatctgt
     7681 ggcatctggt gtgtggattg tattatttct ggggattttt acaaggggcc atgtattatt
     7741 agaaaatact gtttccgtag gattttcgta aataagccgt ttataagcat tctcgtacaa
     7801 aaagtatggt aagataggat tttttgtacg aaatttgttc aaaatatttt attaacgaaa
     7861 ataataattt ttttaggctt gaaaaaacat atttttgtat tttatttgtg aattttgagg
     7921 tttaaaatac aaacgagaac tcccgtgaac cgaatggaat gttattttat tcgattcacg
     7981 ggagtttttt gcttttatga atttgagtga cgtgtcagcg ttctgttgct gcgccatgct
     8041 gccagacgca ccagtccatc atggatcccg gcggcacagt aaggcatcag cagcaggctg
     8101 tacacgaaaa tatagcggga attggcttcc cagatcgtgt ggaacaggaa accgcccagc
     8161 acggcgatca gtcccgtata ggtgtagagg cagaggttgc ggaaacggaa atcgtcgttg
     8221 gctgcggcat tcgcggagcc attgccggga cggcgctttc tgactgtagc aatacagaac
     8281 aacagcatgc ccagatacaa cacattctgc caggcattgc cccattcgat ataagcagag
     8341 gcaagagaac cttcatagac ttccttcaaa aagtcgcttc gcccaccgta ggtggccaga
     8401 gtggcctgcc ggctggcata agtaccgtct gcccattgag acagatgctt gtggagatag
     8461 aagttcacgg catagccggg atgctcccgg aaataggcta tacgctcgtt gatcgccgcc
     8521 cggctgattg cattggctgc gtcggaattc atcccggcag catcataggt atcgatattg
     8581 aagccgttgt accagccgta tccccgggaa gattcctgca tccccatggc aaaataggac
     8641 atggcggtga caccggaact taaggtattg ccggcttttt tctcgtagca cttctgcacc
     8701 agcggcagga ctcccacgga ggtaatggta agacagactg ccatggccat caggccgatg
     8761 cacgccctgc cgctgcgtcc cggacgcagc gcttcgaaga acagcaccag cagcacagca
     8821 ataatgagga tcagggagtt cttgcgcagc atcacactta aagtaaggaa caggatactg
     8881 ccaagggcgg taaagaccgt aactgcggat aatgcgtggg aagtacccac gacgcgggtg
     8941 tcgtccgggg aagtggtctc cacggaatgt gccggaatac agtctgccaa cagctttaac
     9001 agaagaaata gtcccacaga aagcattgcg aaggaaggaa tctcgccata gacgaaggaa
     9061 ctgtacagga tcataggcag gttacagcat accagaatca gataacagca ggagaccggt
     9121 ttccaatcat ccggccacag atagcgcagg gaacggtact ggaacagaac ggccacgcac
     9181 agcaggcaga catagaccag cttgacaaaa tgccaggcgg gagcgctcag cccggtgaga
     9241 ttccacaggc ggagcagcag ctccagaaag gccatcaggc cgatctgctg tggataatag
     9301 gacagataag agaaggtggg gtggataata tctttatttc caaggatcca ttcagcggca
     9361 gcattgtata cagataaggc gtccgcagca ggaactgtgc ggccgaagac gatgagcatc
     9421 ccacccagaa gcaggatcag ccccagagta aaaacaagca gcccgcggcg gaatttcaca
     9481 ccggccttgc cggacagaga cagacaacag gcaaataaaa gcccgtatcc ggcaagctcc
     9541 aacagattcc acaagggatt gtcaaagcgc agaagcacct gctgggtctc catgttttcg
     9601 gcataacagg tagtgaggaa gctgccaata aacagaaaaa ctgccagcag cagtgtcagg
     9661 atctttacga tatttacaga cagacgataa aacttttgca tgggaaaagt cctttcttgc
     9721 cagcaagcac cggcgagaga caggaagtct tccggaactt ttgaacgctg cattttcacg
     9781 cttgattata gcataaaaaa acagtggctg taaatcaata agcgtggtac aatagtttta
     9841 tatatgcaaa aggaaagctc gatttatcag gcggttttgc aaatttatgt gatgctctga
     9901 atgataaaat gaaatacgag gaatggaaaa tggataaaat tgcggtattg attccctgct
     9961 ataacgagga aaagaccatc gccaaggtgg tgtcggatgt gaaaaagttc ctgccccagg
    10021 cagtggccta tgtatatgat aacaattcca ccgaccgcac tgtggagctg gctacggagg
    10081 caggagcaat cgtacgctat gaatatgcac agggaaaggg aaatgtaata cgccggatgt
    10141 tccgtgagat cgatgcggaa tgttacctga tgatcgacgg agatgacaca tatccgctgg
    10201 attgcgcaca ggagatggta gacagggtat tgcagcacca ggcggatatg gtggtgggag
    10261 accggctttc ctctacttat tttacggaga acaagcgccc gttccacaat ttcggcaaca
    10321 gtctgatgcg ggcggggatc aacagcctgt ttcatgccaa cattaaggat atcatgactg
    10381 gctatcgcgc tttcagctat gaatttgtaa agacgttccc ggtattttcc aagggattcg
    10441 agatcgagac agagatgacg atccacgcgg tgaactacaa catgcaggtg gaaaacgtag
    10501 tggtggaata ccgcgatcgt ccccagggca gtgagtccaa gctgaatacc tacagcgatg
    10561 gcttccgtgt gatccgtaaa atgatgcagc tgtacaaaaa ttacaagccg ctgcactttt
    10621 tcggtatgct ggccctgtta ttgatcgctg tttccgcgat cctgttcgta ccggtcttcc
    10681 tggaatatct gaataccggt ctggtgccca gattcccgac actgatcgcc tgtggcttta
    10741 tcctgctggc ggggatccag tcgttttttg ccggtatgat gctggaagtc atggcggcca
    10801 agggccggaa agcttttgaa tatcgcctga atcgcgtcct tggggcgctt caggtgcgta
    10861 gtgggttgta atatgcagga atagaaaatt cttcccagac gcaaaggcgt tgtattgctg
    10921 agtgttctgg gatgtctgtt tgtatttttt gcaaaaacag aaaaagacct ggcatcttcg
    10981 ggaaacatcc tgtggacggc aggctacatc ggaagtactt tgctgtggac ggtagtgctg
    11041 gggtgccttc tgggatttct tggaacctgg gtggtgcatc gactttggaa ccgggcacag
    11101 accgccgaag acaccgggaa aaagcaggct gcatggtatg cccgcctttc cacctggctg
    11161 cagcgccgct ccggtgtgca gatatttttc ggaagtctgt tgtttctgct gttggcctgg
    11221 ctgccggtat atctggcata ttatcccgga atctgtgcct atgatgcacc ggtgcagacg
    11281 ggacagatca tggaacatta ttatttcgat catcacccca ttgtgcacac tctgtttctg
    11341 caggggatgc tgtggctggg aagtcatatc ttcggcagtg tgaatgccgg aatggcattc
    11401 tatacggcag tacagatgct gttgctttcg ggaagcatgg cttatggaat gctggtactg
    11461 catcaccgga aaactgccgc agggtggcgg ctggccattc agttactggg gatgtttttc
    11521 ccgtttcact ggtatatgag tgtcagcatg actaaggaca cggtattcag tgcatttttg
    11581 ctgttgcagc tggtcagtct cgcagatctg ctgtgggaag accgcaatgc ctggcggccc
    11641 gggatccggg atctgctgtt tgccttggga accgtgggga tgatcttatt ccgtaacaac
    11701 ggaaaatatg caatgatcgt attgttggct tttacattcc tgaccttttg tttcggaaaa
    11761 aaagcgagga agctctgggg gagactgctg gtggtatgcg gagcagcttt ctgcgtaggg
    11821 ctttttgtgc tgtctgcggt attttctgcc acgcatgcgg aacagggaga ccgccgggag
    11881 atgctgagca tgccgatcca gcagttatcc cgctgtatga tctatcacgg gggagtcagc
    11941 gtccttgcgg aggatgacaa taccatggat gcggcggaca aagcactgat caatgatttt
    12001 attctggatg aggcgtatcg ggattatgat cccggaattg cggatcctgt caaaagacat
    12061 accaacactt atgtggcacg ctaccgttcc ggtgaattta tccgggtata tctgcacctg
    12121 ctgacgcagt atccgggaga tatgatcaat gcggcactgg ctaccaatgc cgggttcctg
    12181 tctccgtttg ataccaccca tgcggatgtg aaccgtgtgg agggcagagc aggattaagc
    12241 tatgtgcaga ccagatggga agaagacacg ctgaatgaca gaggaatcta caaggacagc
    12301 aaatggccgt ggctctttga acagctggag agctgggcgg agaacaacag ttatctgcgg
    12361 actccggtat tgaaatatct cttcgtgccg ggaagctatc tgtggctgta tctggccctg
    12421 gcagcagtgc tggtgatcgt agacaggaag cggttctgtc tgccccttgc cattgtggca
    12481 ggctattacg ggaccatgtt gttcgggccc accgtacaga tgcgatatgt atatccggtg
    12541 atgctggcgc tgccttatgt actggcgctt gtcaccggca ggaggaaaaa tggataaact
    12601 gatcatcggt gcgggactgt atggcttgta cgcggccctg tactgtggaa aaagaggaca
    12661 acaggtagag gtactggaga tcgaacaggc tccttttacc agagccacat atatcaatca
    12721 ggccagagtt cacatgggct accattatcc cagatctctg tccaccgcta tgaaatcagc
    12781 ggggtatttc aagcgttttg tggaggacta ttccttctgt attcatacga agtttgagca
    12841 gatttacgcg acttccagcc atttttcctg gacagacgca accgagttcc gcaaattctg
    12901 tcaggatgcg gggatcccct gcaaacctct tccggtggaa gagtattttc agcctggctt
    12961 gtgtgatgga gcgttcctga cggaggaata tacgtacgat gcccatattt tgcgggatta
    13021 cctcatggag gagattgcaa aatatcccgg cataaagctg catttcggac ggaagatcac
    13081 ggagatcgaa aagcagacgg actgttatga ggtgacggca cttcacgaag gaaaacagga
    13141 ggtctaccgg gctccctttg tcctgaatgc cacctatgct tctgtgaatc agatcctgag
    13201 gaaagtaaag ggcgcagaga cgcaggattt cggactgaaa tatgaactgt gcgagatcat
    13261 tctctgcaaa gccagcgata agatccggaa tatcggtttt acggtaatgg atggcccctt
    13321 tttctccatt atgccgtttg gaaagaccgg ttatcattcc ctgacttcgg taacctttac
    13381 tcctcataag accagctatg atgcgacacc acagtttccc tgtgtgggag agaatggcaa
    13441 ctgctgtcag ggaggttttc tggggaactg taacgattgc cccggcagac cggagagcgc
    13501 ctgggagtat atgtccacac tggcacggaa atatatgggg gaagagtatg cgttcagcta
    13561 tgagaaatct ttgttttcca tgaagcccat tctgaaggct tcggagatcg acgattcgag
    13621 acccacagta atccgggctc tttccgaaga tcctgttttt atcagtgtat tatcgggcaa
    13681 gatcaatacc gtaaatgatc tggatccgtt tttagattaa tcgcgaacaa aaaggaaaca
    13741 gtatggagaa agaagcaata cagaataaag agaaaaattt tgtatcctgc gtgctgtatc
    13801 tgcacaacga tggaaaaaat atcagagagt tcctgaagac tgtctgtggg accatggaac
    13861 agaattttga aaaatatgag atcgtctgtg tgaatgactg ctgcatggat gataccattg
    13921 aggagatcca ccggtatctg gaagaaagca atacccgcca tgtggtgagc ctgatcaatc
    13981 tgagctttta tcagggggtg gaaatggcca tgaacgccgg aagagatctg gctgttggag
    14041 attttctctt tgaattcgac cgttgtctgc tggattttga accgtccctg atcatggata
    14101 tctatcataa ggcattggaa ggctatgacg tggtggctgc ggcaccgaag cgggatgtag
    14161 catttacctc cagacttttc tatgcggtgt acaatttggg aagccgtaat acccataagc
    14221 tgcgtcagga acgtttccgc attatttcca gacgggcagt gaaccgtgtg aaccagatga
    14281 atgcgtatat tccttaccgc aaggccatgt atatgaactg cggactgcgg gcggagacta
    14341 tagtctatga caataaagag atggcaaagg gaaccaggaa ccgggaagag cggaataccc
    14401 gcagcagtct ggctctggat acgctgatca ttttcacgga tgtactggag aaattttcca
    14461 tgctgatcag tagtattctg tttgcagtca tggtgatcat gttcgggtgg atcgtttatt
    14521 ccatcttcag cacggtacgc ccggtggaag gctggatgag cctgatgact ctgatttctt
    14581 ttggattttt catgatgtcg gtgatgctga cattgatctt caaatatctg tccgtcattc
    14641 tgaatatgag ctttaagaga cagcgttatg taatagaagg agtggaaaaa ctgacaaaat
    14701 gatgtttacg ataatttttc cggtattaaa tgagcgtctg cggctggaaa gcggtgtgac
    14761 acggacggtg gagtatctgc gcaagatcgc atttgaagat tatgagatca ttattgtgga
    14821 taacggttcc gaggacgaga ccccgcagat cgcggagatg ctctgtcaga aatatgaaaa
    14881 agtaagatat gagagaatca atgtccgcgg tgtcggtgcg gctttccgca gaggtgtgga
    14941 attgagccac ggtgacgtgg tgggatatat ggacattgat ctgtctacca atatcaaaca
    15001 tctgggagag gcaatccatc ttttggaaac acagcctcag atcgaataca ttaacggcag
    15061 ccgtttcgcc agagaatccg atacaaaggg aagaaaatgg tatcgcaaga tcacgtccca
    15121 gggactgttg attctgttga aactgttcct gcggatgaag tccacggatg ccatctgcgg
    15181 ctttaccttt gtacgcagac agactgctct ttccctgatt gcgggctgca gtcaggataa
    15241 cggctggttc tatatgatcg aattcctgtt acgggcggag aagcggggcg tgaagatcct
    15301 ggattacccg gtggagtggc aggaagatta caatactacc gttaaggtat ttaagacgat
    15361 ctgtaattat ctggtacaga ttgcaagatt gtttaacgag ttttatatta agaagcatat
    15421 ataagatgcc aggggaaaaa ggtctaagcc cacatgaact cgctcataag tgcgtagcac
    15481 gggacaatcc gtaggcatca aggttgaggg cttttgcccc ggaaacggaa gaaagaagga
    15541 taataccatg gcacatatta aaagaatcga tctgacaaga gatgtggagg cgcatgcgga
    15601 agcctaccgg aaagccattg agaacgtatg taaagagacc gctttttccg gcggaaagta
    15661 tgcggatgct tttgacaaag agtttgccgc ctatgtggga agcaaatttg ccagtggtgt
    15721 caacaacggt acctcagctt tgcatctggc aatgctggca ttgggtgtag gccccgggga
    15781 tgaagtgatc gttccggcca atacttatat agcaactgca tggggggtaa gctatacagg
    15841 agccactccg gtgttcgcag actgtacacc ggatacctgg gagatcgatc cttccgtact
    15901 ggaggctaag atcacggaaa agacgaaagg cattatcggt gttcacctct acggtcagcc
    15961 ttttgactat gcgggagtcc gcaagatcgc agaccgtcac ggactgtttg tggtggagga
    16021 ttgtgcccag gctcacggag caaagtttga ggacagaaat gtgggaacgc tgggagacct
    16081 ggcctgcttc agcttctacc cgggcaagaa cctctacgcc tttggtgagg gcggcagtgt
    16141 tacctgtcag gatgaaaaat attataagca tatcgaccgt ctgaagaatc aaggctgtga
    16201 tgtgcggtat tatcatgatg agatcggtta caactaccgg ctggaaggtc tgcagggggc
    16261 agtgttgagt gtcagcctga aatatctgcc ggagtggacg aagagacggc aggagatcgg
    16321 acatcgttat ctgaaggaga tcacaaatcc gttgatcacc atgcagagac atccggagaa
    16381 caccactccg gtcttccatc tgtttgtcat cacggtgccg gatcatgaag actttatccg
    16441 gtatatggca gcaaatgatg tggaatgtaa catgcattat ccggtaccct gccatttaca
    16501 gaaggcttat gtcaatctgg gctatcggcc gggagactgt cccaatgcgg aatatctggc
    16561 cgcacactgc gtgaccctgc cgctgttccc ggagatgcgg gaggatgaga tcgccagagt
    16621 gatcgatctg tgcaatgcct acagacaggc ataaggagtg aatattatgc aggataccat
    16681 gcggacagaa ataaaaaagc ggatagcgcc ggaacggatt cccggagttt accgggatac
    16741 cggggcagga atctatttac ggctgatgac ccgtgaggat accgaccgga tcgtggcatg
    16801 gcgcaacagt gatgccgtgc ggaaaaattt tatctatcag gctcttttta ccagagagtc
    16861 ccatgagaac tggatccgga cccatgtgga gaccggcgat gtggtgcaga tgatcatctg
    16921 tgatctggca aacgacaagc cactgggatc cgtctatatc cgggatattg acagacatca
    16981 taacaaggcg gaatacggta ttttcatcgg agaggccgat gccagaggca gaggtgccgg
    17041 tacggcagcg gcaaagctga tgctccggta ctgcttcgag gaagaacagc tgcacagagt
    17101 gtatctgcgg gctctgtcgg agaacgggca ggcgatccgc agctatgaaa aagcgggatt
    17161 tcgcagagag ggcctgttgc gggacgatgt gtgtattgaa ggcgactacc gggacatcgt
    17221 ctggatggca gcagtgaatt ccggaaatga tcaggaaaac gaggaataga acacaattat
    17281 gaaaaaagta agttttgtaa tcccctgtta tcgctcggaa cataccttac cccatgtggt
    17341 gaaggagatc cgggaaaaaa tgcaggaact cacgcagtat gaatacgata tttttctggt
    17401 gaacgatgct tccccggaca ataccatggg aaccataagg gatctgtgtg ataaatatga
    17461 caatatcaag ggaatcggct ttgcccggaa tttcggacag catgccgcac tgatggcggg
    17521 acttcgtcat tccgacggcg attacgtggt gtgtctggat gatgacggac agacaccggc
    17581 ggatgaagtg gacaagctgc tgggcaagct ggaagagggt ttcgatgccg tttacgccag
    17641 atacgaacat aaacagcatt ccgcattccg gaatatggga agcaaggtca atgagctgat
    17701 gacccggatg atgctggata aaccggcgga tctgtatatt tccagctatt ttgcagtgaa
    17761 gagattcgtg gtggaggata tgatccgcta tgagaattcc tacccctatg tcattggact
    17821 ggtgctccgg gccaccaaaa acattaccaa tgtggcggtg tgtcacaggg aaagagagga
    17881 aggcagttcc ggatatacat tgaaaaagct gctgggctta tggttcaacg gatttaccgc
    17941 attctcggtg aaaccgctgc gaattgccac tacactgggt gtgggatgtg ccggtatggg
    18001 cttcctctat ggaatctata ccattatcaa gcgtctggtg aatcctaatg tgcccatggg
    18061 ctttagtgcc ctgatggcag ccgtggtatt tttcggcggc atgatcatga tcatgctggg
    18121 actgatcggg gaatatgtgg gacgtattta catcagcatg aataattctc cccagtatgt
    18181 gatccgggaa aagatccatc tgggtgagga agaccggtaa atcgaaataa aacagaaggg
    18241 taacaggaaa acggatgaag aaagaacgtt ttgtggattg gtggaaacag gacaaacgtt
    18301 atatgacact gctgaaagcg gtactgatgg cactgcttcc cctgctctgc tgtctggtac
    18361 ggacggcagc ggaaggaaga agcatcggac aggtgtatct tccctcttcg gaatggaacg
    18421 acgaattatt ctatttcaag caggtggagg gaatcgtaaa ttacggtttt ccccggggat
    18481 atttcggttt taacgaaagt catgcgttgc agttgtcctt tgcagcatgg agtccggtgc
    18541 tggtatttcc ctggatcctg tggggactgt tattcggctg gaatctgctg tctccggtga
    18601 tctgcaatat cgtgctgctg acaatcacca tgtttgtatt tgtctggctg gtgaagccca
    18661 cctggaaaca gctggggatc ctgacggtat tgttcagcct gtattccctg tttgtcagat
    18721 atatgctgtc cggtatgccg gaggtgatct gcttcagctt gctgatcctg ttctacggac
    18781 tggccatgag ctatctgaaa aaggagagcc gcgggaaact gatcgccatg ttcgtgatct
    18841 cggtattgct gaccctgatg cggccttata tgctgctgtt tctggctctg gcctgctatt
    18901 tctggatctg ccgtaataaa aaggcagggt ggatcggatc gattctgata gtggcggcaa
    18961 cgggaattac ctatgccctg atcaaacatt atctgggagc ggaatatttt acaccgctgt
    19021 tttatacgga ctggatcact acctttttca ctgatgggat cggcgcgggc ttccgcaacc
    19081 ttttcggaac actttactgg aaaggactgg aattctacag acattgtatc gaagggggac
    19141 ggaatggcct ggcttccgga gccttttttg acggttatct gttggtactg ctgatcctgt
    19201 tggtgcagag ttttctggac atccgcacac tgcgccgggc gaagcgggtg gaacggatcg
    19261 cgatggagcc ggaggataag aatgtacggg aggcgttgtt cggaccgtac acactgacag
    19321 aggacagaaa acgggaactg cacaaccagc tggttattga agtgcatttt gccctgagcc
    19381 tcatcgccat gctgttcgca catttgctca tgtacaaaat ggtggaaggc agcaaacatt
    19441 tcctgacctt tatcgcagca ggcattttca tcgtcagtat gctggagacc agatattata
    19501 agaaagcggt gcttctggga gcggctttcc tgtatctgta cagcttcaaa gcggtggagc
    19561 cttatgacta tcagattccc tatgtgacag aggaacggca ggcacaggtg gagtactggc
    19621 gggaaatctt ctcggaaaaa ctggccatgg acacggaaca ggtgccgaat tatgagaatg
    19681 tagtgatctg gaccttcgga gatgagaccc cggaaggcca tcagaatctg aaatggcagc
    19741 ttttgtacag tgcaccgaag ggctttggca tcagctgctg tgaacgggaa tatattacgg
    19801 agcatctgac agagctgcag agtcgttatc tggcaacggt gagcggtgga gccatcgatg
    19861 aaatgtgccg gacggcaggg tatgaagagc tcggcaggga tgcggatatg gtgctgtacc
    19921 ggagatactg ataaaagatc gaaagatatg gaaaaagatt ttgaaatctg atatgtgaaa
    19981 aaagacagaa cgggaaggaa ctatagacgt aatggagaca cagagaagaa aacagcataa
    20041 tttcacagag aacaatatgt gggcactgct gtggcatgga ctgccggtgg cggcattgct
    20101 tctctgggga gccttctgca ttaccaacag cctctggtat gatgaggctt attcggcatc
    20161 catggtatcc ctgccctgga agcggctgat ctatattaca gccacggatg atcactcacc
    20221 gttttactat gtgctgttaa aaagcttcta tcatctgtgt gggggaggaa accatttctg
    20281 ggcactgaag ctgatgtccg tgctgttcat gctgggatat atgctgctgg gaaaatatta
    20341 tgtggcaaaa ttgtttgacc gtaagatttc agtgtatttt atgctgtttt ccctgctgat
    20401 gccgatcttc agtgtacagg cgggaaatgt gagaatgtat gcggtggccc tgttctttct
    20461 gacgttgacg ggactgtctg cctatgacat tttcagagag ccggcccgga aaaaatggat
    20521 cgtattctgc atcgccagta tcggcacggt atattgtcat acctttgcat tgatccagac
    20581 gttcttattc tatctgctgt ttttcgcggt gatcctgatc tgccataaga aggagctgat
    20641 caaggggtat tttatctccg gatttacggt ggcgttggtg ttttctccct ggctggcggt
    20701 gacgatccgg caatttgtcc tgcggatgcg ctatgatgac ggttccactg cggaactggc
    20761 aacgctgtat tccgtgatgg attattgtaa ggaatggttc tcagcggtgg agactcccat
    20821 cggcattgtc gtactgttgg gaatggcatt atgtctggta ctcagttacg gagccgtgga
    20881 ctgggtgaga cagaatcata acattgcacc ggccatcgcg tttagcacat ttgccctgac
    20941 gggtatcgtg ggaggcgtga tctccgctac ggtcaataac tgttttatgg gccgttatgc
    21001 gttcccgggc atgggttttg tcatgctgtg gtatgccgtg ggatttgcac agatcacgga
    21061 gaacacaaaa ggcaaaagca ggaaaatatg ggctgcaggc cttctgggaa cggcaggtct
    21121 atgcttcctg ttacagtata cctccgagat caggctggaa tatgatgacg gtctggaaac
    21181 ctacgagaat ttcgtggagg aatacatgac ggagaatgat gcgatcatcg gaccgtatac
    21241 gcatacgatc tttttgaatg tgtatcatcc tgaactgcat tactacacga tcgcatataa
    21301 gctgtacagt cttccttttg tgaatacgga agcactgagc agttacagtc agctggacac
    21361 ttatgataat ctgtggtata tctgcttcca ggggggctac cccaatgaaa tggaagatga
    21421 gtacagctat gaacaggtat tggaatttca ttatatgtat tatgattttg caatcttccg
    21481 cctggagaag ctggaggaag agtagcacag taatggacga ataagaaaaa ggaatggatg
    21541 aaaagcatgg aaaaagtgat gcagaaatgg aagagctacg ggaaacactt ccagcagaac
    21601 cgcctgtata tggggatcct gttactgaca gcggtgtgtg cctacggcta taaggtgacc
    21661 aatgcaacca tcggcatcga tgatacgcca tccttatatt attttgaaga aggactgatc
    21721 gccattgtgg ggcggtgggt tctgtttttg ctgaataagg tggtatcgct ggcagagttt
    21781 gttcccttcg tgacggattt tgcggcagtt gtgatactgg tgctggcagc agtcgtctgg
    21841 tctgcattgt tttattctgt attgggagag aaggttccga cggcaggcta tgctttcttc
    21901 ggtgctgtct ttctctccag tcccctgatc agtgaggtct ttacttattt cctgcataac
    21961 ggtatcgcca ttggctacct gtgctgtggc atcagcctgt gctgtgtcag ggaatggcag
    22021 agcagtaccc ggaaaatgca aaaaggatcc ggtatccggc agaaactggg atgcctggca
    22081 gtggctaaga tcctgacggc ggcagtcttt ttatggatcg ccatgggatg ttacgagtcc
    22141 tttatgatcc tgtggctggc ggggctgatg ctcttgctgc tgacggagcg gatcgcacag
    22201 ggacgtcagg agaaggacat atttgccaca ctggtggccg gtgcggtggc ggcgcttgtg
    22261 gccattgtgc tgcgtagtgt gatgatcgtg gtagtgacga aagcatttca cctggaatat
    22321 ctgcggggag aggcggtaca gcgttccgtg acggaaatgc tgggctggat gctgcagccg
    22381 ggggctttcg gggagcttgc catgatcctc aagaggacct ttgtgctgta tggtgtgttt
    22441 gcctatgcgt atctgccgat ccggattttt atattatcag cggctgcgat ccttgtggtg
    22501 acgctggtgc gggtgatccg cggcagagat ctgtgggcac ttctgttgtt gcctgccgct
    22561 tatttggcag cgttttctct tctgttcatt gaaggaaaag ccacattgta ccgttcggca
    22621 cagttcctgc cggtcttctg tggatacgga gcgctgcttt ttgtgtatgg gatctggcag
    22681 ctgacgggaa ctttggccca aaaagcgaag aatacggctg gcagaaaaat ctctgctggt
    22741 gtgagggcac ttgcagttct ggtgctggcg gtgatcctct ggaaccagtg tatggatatg
    22801 accagatggt tctatattga caagaaaaaa tatgatgcgg cagtgaagac cgtggatcag
    22861 attgcaatgg atctggaacg ggactttgat acttcaaaac cggtgatctt cacgggaaat
    22921 tatgatattc cctatagcat agttcaggat gcctatgtga gctacagcag ccctgtttat
    22981 tataagatga aacgattggc ggatctggtg gacccggatc tgctggataa atacaatcgt
    23041 ggatccagag gagtgtgggt ggcacagaca ccggcactgt ctgttatcga ctggggacgc
    23101 tatgcctttg attccgatgc ggagttggtg aaattttttg aaatgcacgg gcatcagctg
    23161 gtggctctgg aggacatcag cctctatgcg gcagcggaag aagagtctct ggatctgccg
    23221 gaatatccgc aggaggggta tattgtagat aagggggact atattattgt tcacttctga
    23281 tgaaaaacct gcaataggca aacagatcaa ccggttcctc ttgtggctgg acaggattcc
    23341 tctctggtgg accggaattt tgatcgtggt tgtatttttt tccccctatt ttattcttgg
    23401 ggatggctgc atctttgaga tcaacgacca gctggatgaa agcattatga actatatgct
    23461 tcccgccaga catttatggg acggcagtac ggtatatccg gagatgctgg gcggagtcaa
    23521 tgccagcggt atgcagccat cggccatttt attcctgccg ctgtattgtc tgttttctgc
    23581 cagggtggcg tttcttacac aatatatgat ctgctttgtg ctggcgtttc tgggaatgta
    23641 tctgttggta aaggagagca cggagagcag tattctggcg gtgatagccg gggcatgttt
    23701 ctgtgcgctg cccttatatc cgatctacgg tctttcggaa ttcggcattc ctttggtggc
    23761 atatgccttt ctgtgtctgt ggaaacggaa aagaatcctt cccgcattga tgtgtacgtt
    23821 actgttcggc ctgaccagcc acctggtgta taccggttat gtggtgctgg gattgtggct
    23881 tttggcactc cttgttgcct tctttcagaa aagaaagaat aaatggccgt ttctgggatt
    23941 tgcagagttg ctggtgactt atgtcattgt caactggtca ctgatcctgg agattctggt
    24001 gggagacagc agttatgtca gccaccggga agaaatggtg tcttccgcga ctcccttttt
    24061 tgagactttt tggagcctct tccggaacag cgcacaacac gcaccgtcct tacataaata
    24121 tctgatcctg ccgattgtca tattcctgct tctgggagca ttttgcaaaa aggaagaaac
    24181 ggacagaatg atatacaagg cggctgttat caacttttta ttcctgatcg gcatcgcatt
    24241 gttttatgcg ttttgtcata tgacggtggc cgttgatttc aaaaacagcg tcacgggatt
    24301 tttacactat tttcagatcc accggttcta ttggctgtat cctgcggact ggtatctgga
    24361 gttcgcactg gcggcagcgg tattgtggag aacgaaagtg ccgcataccg acagcaggat
    24421 gctgccggga aaactggtga tcctggcagt gtgtctgttg ccgacgcttc agctgttgaa
    24481 agtgaattcc ggaatgtatc tcaatgtgaa ccagatcaat aacggatccg gaatcacggg
    24541 atatatctcc tgggaaagct ggttttcaga ggatctgatg caggagatcg atgatgcgat
    24601 tggccgggac aagagcacct atcgtgtggc acatctggga gtcagtcccg caccggcact
    24661 gatgcatggt ttttatacgg tggacggata ttccaacaat tatcctctgg agtacaagca
    24721 cagattccgg gaagttattg caccggagat tgagaaaaac gaagaagtgc gggtatactt
    24781 tgatacatgg gggaaccgct gctatctgtt caacagcatt accggcaatt acatgcgcct
    24841 gcaaaaggga aacacactgg tctatgaagg cctggaattt gacatggagg cattgcggga
    24901 actgggatgt gagtacctgt tctccggagc tgagatcggc gatgccgaca ggatgggtat
    24961 ggaacttgtg ggatactttg aaacagaaga ctcttactgg gggatctggg tgtatagatt
    25021 gtaaaggtgc agccgttgca acagttcaac gcaaggccaa ttcataaaag cgtctgcttc
    25081 gcagccggcg ccgcatcgcg gctcatcttt tatttcattg accttgcgtt ctgctcatag
    25141 aatgacagaa tcgttgcctt gtgtgcaagc acccacgcac caattctgcc atcctatggc
    25201 tgaactgtta ccccaacttt cttaaaattt cataagattt tttactggaa gagaaaagtg
    25261 tgctatagtg taactattca tggaatccgc attgcagcga ttctgcggat ggggagtaaa
    25321 tagttactaa attatacggc aataagcaat aatagagatt atatatgaaa gacagaatct
    25381 atatctgtca cacatactat catgtatatg tgactttttt aaaagaactg aaattaaggg
    25441 cggaggcaga tcctgcgaag aaagcagggg ctgctacttt agtgctgtcc aaaatgtcca
    25501 ataatttcga gaacctgaaa tcccgggtgg agagcacggg actgttcgaa gaggtactgg
    25561 aattcgatga gaagcgtgaa gatttcttcc cggaactgga aaaataccgg gaggataccg
    25621 gaagctttct ggggaatctg aagaaccgga tccgctttac gaaggagtac gccagactcg
    25681 aggcttctta tgtgccggta gatcttcgga cttacaagga catttatgtc tactgcgatt
    25741 ccgatcccat cggctactac ctgaatcaga accatatccg ctatcatgcc gtggaagacg
    25801 gcctgaactg tctcaagaat tttgacgcgg cgcgatatga caaccgtgga cattttaaga
    25861 tcaaggcatt cctgtccatg tatctgaacc tgatctttgt tcagaacggc tacggaaaat
    25921 actgcatgga tatggaagta aatgacattt ccgccatccg ctatccctgt ccccgatata
    25981 ttgaacttcc cagaaagccg ctggaagacc ggctgacagc ggaggataag cagctgctcc
    26041 tgcaggcatt cgtgagaaat aaagacgaac tggaacacca gatcgaagag agcaataagg
    26101 tgggagataa aatattgatc ctcacggatc cggtatgcgc actggatgtg agagaacaga
    26161 tcttccggga tattatcaaa atgtatgaaa aagagggcac catattttta aagccccatc
    26221 ccagagacct gctggattac cagaagcttt ttgcggaata tccccagttc gatgcatcca
    26281 tgcccatgga gatgctgaac tttttcccga atctgcgttt taaaaaggtg gtaaccatat
    26341 ttacggaggt aaagggactt ccttttgcgg atgaagcggt acgcctcgga cctgatttta
    26401 tggatgccta tgaggatcca ctgatccaca gacagaatga acagatataa gtttggagac
    26461 cgtcaatgat acagatcatc aagaaattac gtgtcctgct ggacaaaaaa cagaaaagaa
    26521 ccatggtggg actcattatc ctcatg
//
LOCUS       DAWDHD010000019        25301 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_153_length_25301_cov_13.954844, whole genome shotgun sequence.
ACCESSION   DAWDHD010000019 DAWDHD010000000
VERSION     DAWDHD010000019.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 25301)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 25301)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..25301
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153_length_25301_cov_13.954844"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         7325 a         5352 c         6344 g         6280 t
ORIGIN      
        1 tttctggaaa aagagaaaat agaagaatat atttactcaa ttgatctgag tgcacttcaa
       61 tagaatagga aaacaacaaa agcctgaggc ttctttttca gaatcattca ggctttcact
      121 atccgtaatg atcttcacca tggaatcttt gatctgtaca agccatcgac atattctttg
      181 gtaggctctt aaggtctcgg aaatctcggc gatatgattt atcttctccc attcattgtc
      241 tgttagaccg tcctgctgca gactttccca ggcaatgtca caattcttgt cctcgtgtaa
      301 ttccttcata ttgttgtaat aggtcttatc tccggtgacg gcataaggct gtacggcagc
      361 ggtcagcgtt ttggaaccca gacggtattg gttcatatac atggtagctt ccagctgttg
      421 agaattgatc cggctcagat atagattgga tcccacgaac aggatcatca tgacgatact
      481 gatgatcagt atccgatagg tagaccttgt taaaaatata aaaaaagcgt aaaattattt
      541 tgagaaccac cggtgatggc ttgcacccta tacaggaaga tggtactata aaacgtaaga
      601 ggcatgattc ggaaaagaga agataacggt aaaaataacg ataaataaag aaccaggggt
      661 ggttacatga aaaaaagaat agttgccgtg acttgtgtgc tgatgttttg tctggggttg
      721 atcggatgtg ggactgaacg agccgacaaa acgcagcaac tgatccgggg tgcggcaccg
      781 gggtataatg acggtctcat ccgggtgggg atgatccaga cgggaaaaga gtctgactgg
      841 agagatgcca ataccaatga ttatctgaat acgttcacca aagaaagagg ctacgatctg
      901 atctatatcg acggcaattc cagttcggag cgccaggtaa aagccatgta cgatctgatc
      961 cagcagaaag tagattatat tatcctgcag cccatcgtgg agaccggatg ggaggatgcc
     1021 atacatgccg caaaggaagc tgggataccg gtcatcgtag cggatcgtaa aatcgctgtg
     1081 gatgaaacgg aatacgtgtc ctggatcggt tcggattttg aagaggaagg ccggaaagct
     1141 gtaacgtggc tggatcagta tttaacagag cagggacggg aaaacgagac aatccacatt
     1201 gtcctgttgg aaggaaccga aggagctaca gctgccattg gaagaacgaa cggtatttta
     1261 catggagtgg aggaacatcc gaattggacc atcgtagata ggggatgtgc caatttcact
     1321 cagggagaag gccagaccta tatggaaaat ctgctcagcg aaggaacagt aaaggatatt
     1381 gatgttatca tttcggaaaa tgataacatg atctttggtg ccatgaaagc actggatcgg
     1441 aacggcaaga gctatgggcc tgagggagac atcattatga tctcctttga cgctttgcac
     1501 gaaagctttg aaaatatgat ggcgggaaaa ttacatgcca ccgtggagtg taatccgctg
     1561 ttggcatcca cggtggagac ggtcatagaa gaactggaag ccggcagaga ggtggataag
     1621 atctataaca ctgaggaaaa catctatacc tatgaaaatg cagccgacta tatagatcag
     1681 agaaagtatt gatttccgag gattacgaat gaaaaaatca ttaaaatata aaatcgttct
     1741 ggctattgtt atcatcgtat gcattatgga cgtgagcttc atatttgtaa actacgtcaa
     1801 ttatatgaat gtcaatcgga attacacaga ttctctggcg cagactgtgg cgaatacctg
     1861 caggctggtg gtagacggag acagcgtaac ggactatctg gacaacagac ggagaaatac
     1921 ggcttattac gaggtatgga ataaactcat tgactatcgc aacaccagtg aaaatgtatt
     1981 gcagatcagc gtggtcaatt tcagggatgg cggaggatgt tatgtgtatg acacagatct
     2041 gacagaaaac ggagcctttt taggagatat cctcccctat gatgaggtac agaatgctat
     2101 aaaagacgag ttgatcgcat gtgagaaaat agaaccgttg gagtataacg gaagaagcga
     2161 ttactacatt ccgctgaatt cctcatacaa tattccggtg gcttatatta tcgtaggaat
     2221 ttccacagaa aatgtcagat gggagcaatt gacttattta gggtatatta ccatcattgt
     2281 tacatcgatt acggtgcttt tcggagtctt tatgatctgg tttatgcagc acaatgtact
     2341 gcgaccgtta aatgccatgt ccaaggcggc ttccagctat tctattgaca ttttgaggga
     2401 tggtaaggaa tctcccatcg gccggatgaa catcagaacc ggagatgaac tggaacgact
     2461 gtgtgaatcc atgaagaaaa tggagcatga tatcattgcg tccagttccc agctggctat
     2521 tgccgattgg aacagcaatc atgacagcat gacgcagctc tacaacaaga gacactacaa
     2581 agaacagtta aaagaactgc agaatgacag taccatcggt gtaatttatt tcgacattga
     2641 caatttgaaa cgcatgaatg atactttcgg acatgaaaaa ggtgatgctg tgatcctgaa
     2701 agcagcggaa tttatccgga ggtatctcac ggagcaagca agaggattcc gtattggcgg
     2761 agatgaattt gtaatgctga tcagagattg tacggaagaa aaagtacagg aactggtaag
     2821 taccatgcgt ggagatgaga aaggaaattt atcggatgta acagcagagt tttactgccg
     2881 tcttgcaatc ggcggagcct ttcgcaagac agcagaaact ctggaagaaa ccataaaacg
     2941 ggcagatgat gaaatgtaca agaataaaca ttccgtgaga aaagcctgaa attttaggct
     3001 ttattgaata gtcagccgga agctggcatc gttgcccttc agccggatcc ggtacagatc
     3061 atgatcccat gccgttttca ggcgtgcgtc cgtgatcggc agcgtttcta tggcgagcag
     3121 atcagcccct tcgaaggagg cagatgcttc tccgatggag aatttaccgt cagaaagaat
     3181 aacaggtttt tcataagtaa tgaaattaag aataacatct tgtgcatttg tgtggtcttc
     3241 caatacgaca gtgttggaag gcttttttaa tgtcaccttg cggctatagg tcagacccgg
     3301 tacaacggct ccggcatcgg gataggcggc agccagttcc atggaaatag aaggttcggc
     3361 tccggtcagc tccgtcacca ctttttccgc acggtattcc gcaccgtcct tctgatccgt
     3421 gccgcagatc gtaggcagat tatgatagcc ggactgcatg gtccagatct cgtaacgtct
     3481 tggagagaag gtcttccggg tgtaggtctc cacaccgata tccgccagaa cgggcagtcc
     3541 atttttgtat aaggtgatgc tgccggtatc attatggttg tgactgtcag cattgtctcc
     3601 ggctttgact gccagatcca gagtatcgga atgcacgagg aacagcccca cgctgggata
     3661 atagacatcc cggtgggaaa gcgttccgga agtgtcctgc ttcatcaatt cctcataatg
     3721 aaatacggtc tgcatacgat agaacagatt cccaccgttc acttcatctg tgacaagctg
     3781 accctgtccc gcctggaaat cttttgcggc gaacaggcag agatcctcct gcccggtggc
     3841 cttgccaaag agatattccc gtactccggc acggccggct ataggagagc agtcagcaaa
     3901 attaaaatag tatttatcat tcacatgcac gttcagaata taggatgcaa tatttttcac
     3961 cttatcccac cggaacagag tgtcgaaatg accgtcggta accgtgttca gcacagtcat
     4021 ggcaccgtat agacatagtc ccgcatggcg ataatattgg gcaccctcct cgcaacagcc
     4081 gtcattgccg taatccttga gaaaataatc acagctttca gcggccttat gcagaatcgc
     4141 ctgcaatgtg acaactgtgt cagacgtatt ctccggcagg aacaacggag catttccgca
     4201 gaatgtgcgc accggagagg acaaccgaga ggacatttcc acactccagg gcattaggaa
     4261 cgtggtcagc agtacattct gtgtacacca tacggtccag ttgcacatag gctcatcgtc
     4321 atgtcccatc caccagaaat gtgcggtcag gtagggcagc aggatccggc gcttcagatt
     4381 gtcctctatg caggtcagga tataggggct gacagcgttg aattcctctt ccagcagata
     4441 agccgcacag gcaagcaaag ctccggtctc gcaggcgaac agatccatga cgggcctggt
     4501 cacatccggc aggatcaact gcggggtatc gcggatatag gagttatgcg cgggaagctg
     4561 ccaggcagtc tcttcacaga tggaatagat accattgaga atatcgtcca gaaaacgtcc
     4621 ctgatgttcc acacattcgg cctggatcag atcattcagg gcattgcgtt tggcaaaata
     4681 gagggcttca aaagcagccc ggtcaccgtc tcttttaaaa cgcatgtaat ctgtggcatg
     4741 tatcaccggg aattcatatc ccagctttgc ctcaccggcc tgaatcagaa gttccttcaa
     4801 tgctgccggg agtccttcat aagcctttcg gtccatagcc ttcggaaatg ggtgaaaagg
     4861 gtggctgttt ccgtggaatg catttgctgt ctccaaaaaa cgcttttcta tacttatcgg
     4921 ttgtgaattc atttctctga tcctgccttt ccatattcat aattcccaat aagagacaaa
     4981 ccgtaatagc agttaactga acttattgtt gacgtattcc gtgggggaga caccctccac
     5041 ccgtttaaat accttgctga aatagaaggc actctcgaag cccagagcct ctgcggcctc
     5101 gtagaccttc atattttctt cttccagcag ccgtttggct tccgtgatct tgcagatgtt
     5161 gatatattca ctgaagccgg tatcgttgta tttgctgaac agctggctta agtaattcgg
     5221 gctgatgccg aataccgctg ctacttcatt cagggacaac cgctcgctaa catgttcatt
     5281 aatatatttc cgaacgttag ttacgatgtg gtttttataa tcctttcgac gggattggaa
     5341 caattcgcac agctgtccgc agaagaattg cagccactgg attacgtgat ccacattgga
     5401 ctgcttatag agggaacggt agccatccgg atctcccgca aaaaagccgg acacaataga
     5461 ttccccgtcc tgtaaaagcg aaatcgacag atagagaata ttgctggcag cgtccagagc
     5521 ctgtacataa tgtcccggat gatccttgaa cagatcgcag agggactgaa ttgtagtctg
     5581 cagaatatcc ggatcatact cttcaaaggc tctggtgaga tcatttttaa acaggctgat
     5641 gttaaaagaa ttcttggcgt tttcctgcga atgatcggta tcgaaagcta taattgcgtc
     5701 cttgggttcc gtattccgga agatctgtct cgcgtactgg taggagtcac agatggtagc
     5761 gggagtctga accgggatgc cgatgccgca acgcagtgtt acattatagt aattctgcag
     5821 tgttccgctg atgctttgca gaatgtctgt cagactttcc acataatcat cggtatcgga
     5881 ggattccgca aagcagaaaa tcagtgcaaa atggcgcaga tccagagaca acgcataaag
     5941 aggcagatat ttagccccca gttcccgtat catctgtaac gaactggtga acagaggcat
     6001 ctgtttttcg gcagacatct ggtcggcctg agggctgatg atctctccgt aacagcacac
     6061 ataagcaccg taatcgaaat tcagattcag atctctgctc tgcagacgga actgttcttc
     6121 ggactcgaac aggtcatgca ggagactgat caggaatttg tcgtaaaaag gatgaatatt
     6181 tacggcggac agctgctttt tccgagattg ggaaatctgt gtgagcaccc ggcctatggc
     6241 atttttcagt acttccggtg tcagttccag ctttaccaga taatcggata cctgataaga
     6301 caaggcatcc cttgccatct ggaagtcttc atagctggtg aggatgataa aataagggca
     6361 gttatcccca tagcgttctc tgcatgtttt cgcaagctcc agaccgttca tgaccggcat
     6421 tttaatgtcc gtgatcacga tgtcagtaga tttttcttca ataatcttca gcgccgcctg
     6481 accgttcatg gcagttccac agatctcgat attcatttcg ttccagttca gcatggaacg
     6541 gattccagcc tgaaccagag gttcgtcatc cacaattaaa agctgataca tagtaactcc
     6601 attctggaca gcttcctgtc cggattaagt gttttcagat gaaatagttt cggatgaaac
     6661 agtttcattg ttatgcagag gaatattcgg tatgtgtatg gacatagtcg tatattcccc
     6721 ttctttgctt tctatggtaa tgccatattc tgcaccaaat tggtattgta agcgtttatg
     6781 cacattgctg acaccgattt cccggaagaa atccgcggaa ctgtcgtcgt tggagcgtaa
     6841 tacctgtgca gctttatccg ctgtcatgcc tacaccgtca tctgtgacat caatacggat
     6901 cttttcggtg ttgtccgatg tctcatagcc gacatgtatc cggatgtgtc cggcacagcc
     6961 cttcggttcg ataccgtgga aaattgcatt ttccaccaga ggctgcaatg taaacttgat
     7021 aatctcactg ttgtacagag attcattatc cacctgaatg tccatggtaa tggtgccgcc
     7081 gtagcggtag ctctgaatgg tgaaatagtt ctccaacaga gataattctt cccggatatc
     7141 gatcaacagg ctggtgcctt tggaaatact ttttaacaga cgggacaggg aggtggtcat
     7201 ctcggagata ccggttgccc cctgtatggt tgccatccat ttgatagagt tcagtgtatt
     7261 atacaggaaa tgaggattga tctggctttg cagcatcttg tattccagat cctgtttctg
     7321 tttctcatct tccagtcgtt tctccataag ttccgataca ttttccgaca gatcattgat
     7381 tccccgtccg atatccccaa gttcgtgatc ccattccaca gaagggtctc tggagaagtc
     7441 tccgccggcg atgcgtacca tccgtgtctg cagcctggat acaggtaccg tgatcatacg
     7501 attcatcaac agcatcagaa cgacaccgat agacaaaatg ccgatcagga tgcaggcaag
     7561 gattcccatg aacaaaaccg cctgattggt ccgctccgca tgtgaaatac tctgacttaa
     7621 gtagcagtcc ggcatattta acggagtggt gaccagaatc tgttctccca tggtagcact
     7681 gtggattttg gaaatctgtg tggtgcccgt gagagaatat gtgctcaggt catccagaat
     7741 actatattcg ggcgtaaatt cctgcaagcc tccgttttca taaatatagg tatgtcctgc
     7801 aatggacaga tagagaaagc tgtctgacgg acaactgtaa tccttcaaag gtatggtgaa
     7861 cagatctgcg gatatttcga tgaacagata tcctgcctgc ctgttattgt attggtaggt
     7921 aatgggacgg atcacaggta atactttacg tcccgcacgg tgaaaaggat cggatacaat
     7981 accgggcgaa aaatcataat catcggcagt cagtaattct tcataataag gaagctccgg
     8041 aatcagcgtt gcaagatctg cggtagaaga gtacgttgcg gttaccacct gcagataatg
     8101 ctcctgtgag acaatggcaa ttctgggcat ataattataa gaagaattcc ggctgcattc
     8161 ctcgtagaag gaatcataag tggatacgga caggacagag ccgggattgg ggttatgttc
     8221 aatataattg gcaatattgg aatctgtctg gcaatatctg accaaccggt acacttcatc
     8281 aatactgttg tccatggatt ccgacaacag tcgcaggctg gtctcggtgg actggatcag
     8341 agtgttctgc agatagtgcc ggaatatgta ataactgaac aggctgatca acaggcacat
     8401 tcccaaaatg gcactcagcg tgaaaatcaa tatgcgattt cgtatgcttt ttgagtgata
     8461 aacagtcttt ttcatgcatt cagtataaca taacgaaaga aaaaaggtag ttggaaaaac
     8521 aagatactaa aaaaagtgca tgttaaaata aaaaaataca ttttctgaaa gcgctttctg
     8581 aaaaaatgta aacaaattat aaaaaattgt gaatactatt ataaattttt ccatgtcatg
     8641 tctcggcctt tttcgttata ctttagttac agtcaaggaa ccgataacaa cggattcgta
     8701 acagacgtaa catagtatca aattaacagg agggtttaca tgaaaaagaa agtattaagt
     8761 ttagtactga cagcagcaat gatgaccacc atgcttgcag gctgcggcag ctccgataac
     8821 ggcagcacag cagcttccac cggtgaggca cccgcagcag caaccactga ggctgcagca
     8881 gcttccacgg aagcagcacc cgcagaagaa gttactttaa agtgggctat ctgggatcag
     8941 gagaccacac agtactgggg tgacattaag gacgcatatg aagcatctca tcccggtgta
     9001 accattgaga tggtagacct tggttctacc gattacatga ccgttctggc aactgagctt
     9061 tccggcagcg gctccgattt cgatgtagta accgtcaagg atgtacccgg atatgcaacg
     9121 ctggtacaga agggatctat tctttccctg gatgattaca tctccaaaga tggcgtggat
     9181 ttaagcaaat acgcaggtgt taccgatcag gtaactgtag acggaagcct gtatgagctg
     9241 cctttcagaa atgacttctg ggtattgttc tataacaagg atctgtttga tgccaagggt
     9301 gttgcttatc ctaccaacga tatgaccttt gatgagtacg atgctctggc aagagagatg
     9361 accgatacca ccttcggctc tcaggtatac ggtgctcatt atcacacatg gagaagtgca
     9421 gttcagctgt tcggtgttct ggatggtaag catactattt tggatggaaa ctatgatttc
     9481 ttcaaacctt actatgagat ggttctgaat caggaagctg acggtgtatg cagaaagtac
     9541 acggatctga agaccgaagg tttacattat tccgcagcat tctccggtgg agacgtagca
     9601 atgatgaaca tgggctcctg gttcatcgca accatgatgt ccaacctgaa gtccggcgaa
     9661 tatgattcca gcctgtgcgg taactggggt atcgttaagt atccccatgc agaaggcgta
     9721 gagcccggtt ctactctggg taccatcacc ggtctggctg taaccactgc aagtgatact
     9781 cccgatgcgt cctgggattt cgttaaatgg gtaagcggtg aagaaggcgc agcagttatg
     9841 gcttccagtg gtaacttccc tgcaatgatg actgatgaag tagtagacat gatctccggt
     9901 ctggaaggat tccccaccga tgaagccagc aaggaggctc tgactgtatc caacctgtac
     9961 ctggaagttc cttacgctcc caatgtatct gagatcaact ccgctctgga ttcctatcat
    10021 ggaagcatca tgaccggtga gatgagcatt gatgatggta ttgctgcaat gaataaagcg
    10081 gtagctgacc ttcaggctca gtaatgaaga tcttctctat aaaagtattg aatgtttgtt
    10141 gaatgttgaa taagacttaa ggggtcgtac ctacggcccc tttcttatca gaaaaagacg
    10201 caaaagatga cacaaaaagc gtttgaaagg agaattgtta tgcggaaatt aagccgtcaa
    10261 accaaaagag atctggtggc ctactccttt attgcaccta actttatcgg tttctgcgta
    10321 tttactttga tccccattat ttttgccttt gcccttgctt tcatgaagtg ggacgggagt
    10381 aacccgatcc agtgggcagg catcagtaat tttacaagac tgtggaaaga tactttcttt
    10441 atcgcagcat ttaaaaacac cattatttat tgtatcggta ccgtgcctct gaccatgatc
    10501 gcatctctgg cattggccat cgtgttaaat cagaaagtaa taggaagagg tattttcaga
    10561 accctttcct ttttcccata tgtagcttct ctggtagcaa tcaccgcagt atggaaaatg
    10621 ctgttccacc cctccaaagg acctgtgaac aacattctgc tgaatgtttt caatgtaccg
    10681 caggatcagc ttccccagtg gttcaccggc ggactggtac tgttctccat gattctgttc
    10741 agtgtatgga aatacatggg ttattatatg gtaatctacc tggcaggctt acagggaatc
    10801 tcctctgaac tgtatgaggc agccagcctg gacggtgcga atacctggca gaaattccgt
    10861 tatgtgacct ggccgcagtt gtcctccacc actttcttcg tagtcgtcat gctgactatc
    10921 aactgcttca aggtgtacga tatcgccatc atgctggcag gcggtggaag cggagaatta
    10981 accacttcag caaccgttct tgtatactac atctatcaga aagcatttat tgactgggat
    11041 ctgggttact ccagctctgt agcaatggta ctgttcctga tggtactgtt cgtaacgatc
    11101 atccagttcc gcggtcaggc aaagagggag aaagcataat ggcaatcttg gctttgaagg
    11161 ttcttgataa ggacagcaac accatctgcg tcagcagtgg tgaggacttc gtggatctgg
    11221 tctgcaccca cacctatgaa gagggtgaca ggatcgtgct ggagaccgat gagaaaaata
    11281 tacatgtaca tttacaggtg gatgatgctc ttggggatgc tttcgtatac ataaccgaca
    11341 atgtatccta ttatgttccc ttcggggaga aaaggatcag catgtcccct aaggtatttt
    11401 ccggaaataa acattatctc tatgcggaag tcgcaagaga agatgagatc acagcctata
    11461 gaaacctggc actgaatcct gcggatcagc atatggatgt tccctgttat cctcatgcga
    11521 cagccaatgt ggagaccaga ggcgagagtg tattcgcagc gaaaaacgcc attgacggtg
    11581 tccgggcgaa ccgctcccac ggagaatggc cctacgaatc ctggggaatc aacatgcagg
    11641 atgatgcagc tatgaagctg gacttcggca gaccggtact ggcagacagg atcgtgttgt
    11701 acaccagatc cgatttcccg catgacaact ggtggcagca ggtaacgatc cggttttccg
    11761 atggcagtga acaggaattt cctttagaga agagcagcag ggcacatgta ctgccgatcc
    11821 ccgagaaaga gatcacctgg ctggagcttt gcaacctgat caaagcagaa gatccatccc
    11881 ccttcccggc attgagtcag attgaagtgt acggcagggt aaaacgttag gaggcgcaca
    11941 gattatgaaa tcagcacata aaaaagccgt ttttggcaaa gtgatcgtat atgcgattct
    12001 cattctgatc gcagtggtca tgatcattcc tttcctgtgg atgctttccg catccatcaa
    12061 gagtgacaga gaagtattcc agatgaatcc ttttgtatgg atccctgcga accccaagtg
    12121 ggacaattac gtgaagatct ggacgaagat tccctttggc agattcgtgg gaaatacggt
    12181 atacttgaca ttgatcgtta ctttcttaca gttactgacc agcagttttg cagcatattc
    12241 ctttgcaaaa ctggacttcc gtcataagaa cggattgttc ctggcataca tcgcaaccat
    12301 cgcaatgccc tggcaggtat atatggtacc tcagtttatc atgatgagaa gaatgggact
    12361 gaacgataag ctgcttgcta tgatctgcct gcaggcgttc tccgcattcg gcgtattcat
    12421 gatgaagcag ttctatgaag gcattcccga tgatctgtgc gaagccgcca gaatcgacgg
    12481 tatgagtgag taccggatct acgccagcat catgctgcca ctgtcgaagc cggcattgtc
    12541 cacactgacg attttcacct tcgtggcgac atggaacgac tatctgggac ctctgatcta
    12601 cttaaagagt caggagaaga agaccattca gctgggatta aaaatgttca tcagccagta
    12661 ctcttccgat tacggtctga tcatggcagg atcggtattg tcgctgatcc cggtgctgat
    12721 cgtgttcctg gtattgcaga aatacttcgt agaaggtgtt gcttctacgg gactgaaggg
    12781 atagcagata acaatgtatt ttcacaagcc tgtgtcggtt ccatgtcaca ggcttgtata
    12841 ataaaaatgc ataataaggt aaaggataag actatgataa catttgacga aaagaaatat
    12901 ccgcagatca cagatgcgga gatcaaagac gctctgcagt tttccacgga gcaggtgatc
    12961 cgggatctgc cggagtttac agagcagttc cagaaagctt acagtgagaa cggtttttat
    13021 cagccgattc cgaacaacta ctggacctgt gggttctgga ccggagagat ctggctggct
    13081 tatgaatatt ctcaggatga aaggctgaaa aaagccgggg aagttcaggt aaagagcttt
    13141 ttggatcgta tagacaagaa aatcgaagtg gatcatcacg atatgggatt tctgtattcc
    13201 ccttcctgcg tggcagctta taagctgacc ggcagcaaag agggcagaga agcagccatc
    13261 aaggcagcag accagctcat caccagatat catccggtag gggagtttat tcaggcatgg
    13321 ggacccatgg atgcaccgga gaattaccgc ctgatcattg actgtttact gaatctgcct
    13381 ctgttgtact gggcatccga cgagaccggg gatccgaaat accgtgatat tgctgagaag
    13441 cacatccata cagcagtagc aaatgtgatc cgtgaggatt attctacctg gcataccttc
    13501 ttttttgata tgaagacggg agcaccggat catggtgcca cctgtcaggg ataccgtgac
    13561 ggttccgcct gggcgagagg tcaggcctgg ggcgtctacg gtatggcact ggcttaccgt
    13621 tataccggaa gaaaagaata tattgatctg ttccggcatg taacagagta ttttctggca
    13681 catctgccca aagatctggt accttactgg gatctggaat tcaccgacgg ggatgaccag
    13741 ccgagagatt cttcttccgc ttccatcgcg gcttgtggta tgctggaaat ggcgaaatat
    13801 ctggagccgg aagaagcaga gcattacatt acactggcaa aacagatcat gcggtctgtc
    13861 tatgtgggct atgccgtaaa ggatccgaag gaatccaacg gactggtgct gcacagcacc
    13921 tactcaaacc attctcctta caatacctgc aatcattacg gcgtagacga gtgcaattcc
    13981 tggggagatt acttctacat ggaggcactg acccggttat tgaaggattg gagagagtac
    14041 tggtagatta tattaaattg atatcatacg aagcccatac agatacgcat gccttgatgt
    14101 aaggtctccg agtgtggatt ttctaaagaa aagtggctta gtttttctca ttatgacggg
    14161 cccgcctaca gtcgacatcc atgtcgacga tcggctgacg cgaacatcgt gttcgctttc
    14221 gccctgttgc cagaccactt ttcaagaaaa tctcacactc tacgacaaac atcttcggca
    14281 tggcatcctg tctgggcttc tgtaagaagt atttctaata taggaatata gttactacgt
    14341 ttgttatttt ggagggacta actgcatgaa cttacatagc aaaaaagatt ttcaggactg
    14401 tatgttatct atcctggagc cgttaaaacc ctattacagt gaagaaaagg cgagattgac
    14461 gttgggcgtt accatggcac attatgacat cggtgccaca tggatggaag ccttttcccg
    14521 tcccttatgg ggactggtgc ccttttgggc aggcggtgga aaagagccgg aattcgaaga
    14581 gatctatcgc aagggcctga cggccggaac cgatgagaag aacccggaat attggggaga
    14641 atgcacctct tttgatcaga gattcgtgga gatggcagcc atttcctatg gcattatgtt
    14701 tgcccccgaa gtggtatggg atcctctcac ggaagcacag aaggagaatc tctgcaacta
    14761 tttaaataag atcaatgagc atcctctgcc ggtgtgtaac tggatcctgt tcgccgtact
    14821 ggtgaacatt gcaatgaaaa aggtgggacg ccggtatagc cccgagatgc tggaagaata
    14881 tctggaaggg ctggagacct tctatctggg tgaaggctgg tatcaggacg gagattccgg
    14941 tcagaaggat tactatattt cctttgccat ccatttttac agcctgatct atgccgtgat
    15001 tatggagaaa gatgacccgg agcgggcaaa aaaatacaag gcgagagcca tggaatttgc
    15061 aaaacagttt atctactggt tcgatgaaga gggtgaggcc attcctttcg gcagatccct
    15121 gacctatcgt ttctctcagg tcagcttttt cagtgtctgc ctgctggcag gactggagcc
    15181 gttcccggtt cccgtgatga aaggtctgat cgcaaggcat ctgcgcacct ggctgaagcg
    15241 tccgatcttc gacagagatc atgtgctgac catcggctat ggatatccga atctgaccat
    15301 ggcggagcgg tacaatgccc ccggttctcc ttactggggc atgaaggtgt ttgccttttt
    15361 gttgcttccc gatgatcatc ccttctggag tgtggaagag gcaccattgc cgaagctggc
    15421 accggcctgt ccccagaagt atgccgatct gtttgtctac cattacggca atcacaccac
    15481 agcatttgca ccaggcgtct acagccctaa cggacacggc cagatcgtgg caaaatacgg
    15541 aaagtttgct tatgataccc ggttcagtat cagcgtggca aagtcctgct acgagctgca
    15601 tgagaatgcc ccggacaata tgctggcgtt ctggatcgat ggttatgtct atgtgcgccg
    15661 gatctgcgag gagtcaaaga tcacggagaa cggagtatgg tcgaagtgga gtccctatcc
    15721 cggcattacg gtagagacca cgatcacccc ggatgccggt ggacatacca gagtacataa
    15781 gatcaccagc gagattgact gcgtggccta cgactgtgga tttgcagtca gcagaggtga
    15841 ttttgaagag gtcggctttg cggaaaaggt ggacggtatg tccgcccagg ccagcaacga
    15901 attctgcagt tgtactgtca gtgcggtatt ggatgaaaat acacaggtta acgaagtggc
    15961 agacaaaccg cccgtaggct acatgatcac cgtagatccc aacaccaacc tgctctacac
    16021 caagaccaag atcccggcag tgaaatacca catccgcaag ggatcccagg aaatcgtcac
    16081 cagagtggat gcggaagtaa tctgaatata tagaaattgc agtggagttt tgctatttgc
    16141 aggctccatt gtttttatat gaaataaaag tattatgtat actgtaaatt gttgaaacat
    16201 aaatattaaa aaagtatata gttgcataat tttttacatt atatgtaaac acttacaaaa
    16261 ataatcataa aaatcataga gaaaaaagga agtaaaaagt aatttattgc attttgtaca
    16321 atctgtctca ttttttataa taaagatgta ttgttataaa taattatggg aggattgtat
    16381 gaagcaaaaa acatgtaaag tagcgaaaac agtattgact ctgttgcttt gtgcaggtct
    16441 gacggcaggt tcggtgccga tagatgcatt ggcagcggat gtacagtcag aacagcctgt
    16501 tgggggcaca gagactgata cagaagccgg aacagaaggc aatacgcaat ccacaggttc
    16561 tgtatctgat ggaaacgtag ttataaagga gagagaagaa acaggaacgg agagtggtgc
    16621 cccacaggcg gattccgtat cagaaggaga cggaatcgca ttaataagcc tggaagcttc
    16681 caatacacat attgcaactt atacaggaaa aattccggaa ggaatcacat gggctgcaga
    16741 tgtgacggta gatactttca gcaagtcgta cagcgtggtt acggcaaagg cggcggacgg
    16801 aaccagctat acggttgagg ttgtaccgga aggaattgta tactttattg acaatgtaac
    16861 atcagacttt tcggaaacta caacagagcc ttatgaagca gtaaagacat tagcaggtga
    16921 ccagctgata aataaagtct atgatcagca aaagacggcg gatactgcat ggggtctggt
    16981 agatacggat gcgacaacca aaaaatataa caatacgact actgataaaa caaccaccgg
    17041 tatttatgga aataatgcca taggagaaac gttgtcttat gatctgtatc tggaggcagg
    17101 cacctatact attacttcag gacatagaga atggtgggac aaaacccgtc ctatggatgt
    17161 gctgttaaaa acagacgcag gagaaacaac cattggaagt gtatctgtta gcaaaagtgt
    17221 aaaaacagca caattttctt atacttttac gctggatact gcccagacgg ttacttatac
    17281 cttaaagagt acggcaaagc aggctcctgt tttaagctgg cttggagtat caaaatatat
    17341 cacaaaggaa tcggaatatt ttggacaggc tctggaagac aataatcagc tcactgttct
    17401 ttctgatgct tccttagaag cagacagcgg cacaggaaag cagctttctg tgacaggtgg
    17461 ctggactagt ggtggtatct ctaaggcagg cgcaaccata aaaaatgcac ctgatttttt
    17521 ccagagaaat cagtttacat ataatctcaa cttaaaatac aattacatac aagaaggaac
    17581 ggggagtcgt gctgctgttt tccttggtaa ttccgattcc actgctttcc gtgtaattcc
    17641 ggcaaagtcc cagagtcagt ctgtgctgag agtgggtacc aatgcaggag caaaggatta
    17701 tcctctgaca acggctctta ctgcagggga ctggcatgcg ctttctattg tgtataccga
    17761 ggatgaacag caaggctatg tggcattata ctgtgacgga gcaaaggtac tgaatgccac
    17821 tggaataggt tttaaattaa gtaatactac caatctggct gccggaatcg gtgcagctta
    17881 tggaaccagc tatttgtgca acggtactta tgacaatatt gttgtcatgg cagcagcagc
    17941 aacagaggaa gaagccatta cggaaacaca ggcaagactg gatgccatca aaggcgctgt
    18001 ccagacagac ggaaacatag tgatatccgg tgcagatgtt gacaaggcaa cagccaatat
    18061 caatggtctg acttataagg gctttggtat gttaaatggt aacagtacca gtaacctgct
    18121 gctggattac aaggcagagc attcagatca atactgggaa atgatgcggt atcttttcgg
    18181 tggagaatat cctttgttta cccatatcaa gatggaaatg ggaaatgacg gaaataattc
    18241 taccggagca gaagcctgta ccatgcgtta tgagaacgaa gaagcggatg cttcccgctc
    18301 tccgggattt gtcatggcag ccgatgcgaa gaagatcaac ccgaatgtca agatcagtat
    18361 tttgcgttgg gagatgccgg catgggttgc ttccaaatgg agcagtgacc gtaccggtgc
    18421 aggatatgaa gcagtttata aatggtacaa ggagaccatt tttgatgcct atgaaaaata
    18481 tggttatgtc gtagatttcg tagatcctga taagaacgag acaacagacc ctaatgagga
    18541 ctttatcaaa tggttttcca accgggtaaa aaaagaaaca gatttcccgg actatatgga
    18601 tcaggcagcg attgacgctt atcacaatat cagaattatt gcttctgacg agaataagac
    18661 cctgcaaatc gtaccttcca tgagaagcga caatgatctc tacaatgcag tagatatcat
    18721 tggtttccat tatcgtacca gtgccacgga ggattacatc aaaatggcgg atgtggacga
    18781 caaggaggta tggtacagcg aggggtgtgc aacctttggt tatacagagt tacaggaaaa
    18841 caagacgagt gaatacggtg gtggaacgat cggaggatat cagagtccgt tggctctggc
    18901 agacagcttt atcaatgcgt ttaccggatc tcgcagaacc cactatattt tccaaccggc
    18961 tatcggttcc ttctatgaag gcatccagta tggccataaa gagctgttaa gtgccagaga
    19021 tccctggagc ggttatatcc attatgatcc ggcactgtac atgattgcac atttctctca
    19081 gtttgcagtc accggatggg aggataccga cccttcccag aatgaaatct ggcgtgttat
    19141 caccggtgca acctccggat ccttcggctc cagtgacaat gagcatcaga cagcagggat
    19201 ggatgggaaa gccagctata tgacactggt atctccggat aaaaaagact tttctgtaat
    19261 ttttgtaaat aacacaagaa atcaaaaaac ctttaagatt actgcaaagg atatggcagt
    19321 agcctccgat gcaaccttaa aaatatggac aaccacaaca gatagctatt tacaagagag
    19381 tgatacagat gcagaacagc agaacggttg ctggtatgta acagttccgg cttatgggat
    19441 ggtaacagca actacactga atacgtcacc tgcaagaaca ccggaacaag aaatccataa
    19501 cgaggacaga acggttttgg atactgacag taccggacgc aatcagaata caacggataa
    19561 tgtactttat gcggatgatt tcgagtatac agaagaaccg gaaatggaac agtataatgc
    19621 caaaaccggg aaaaccacca gtgtagatta tctgacagca agaggcaatg agccacgtta
    19681 catgttggat acgcatggcg cttacattgt agagaacggc agattaaaat cggaactgac
    19741 gaccagtgtg ggacagtgga acggtggaga accttcaacg atcgtcggtg atttccgttg
    19801 gatggactat gttaccacga tagatgtgca gataccggat gcatctgcat cgacctatgc
    19861 gagactgggt atccgtaccc agacaggcat gaactggaac cagagcggct atacgctgga
    19921 gattaacggt gccggctctt ggaagctgta ccgcataggc agtgctgtag caaatggaac
    19981 ggtaacagcc acaacagatg gcaaatatga attaaagctg gcagcccttg gcgacatgat
    20041 taccgcgttt atcaacggag aacaggtcac aagctatcag gacgcatctc cgatgctgtc
    20101 cggtcgcgtc aagatcagca acaactggca tcaggtatat tatgataacc ttctcattga
    20161 gaagattgca ggaggatgtg cttatgcgtt gtctatggtg gatggacagg atgacagcgt
    20221 aagctatgag ggaagttgga caatcgacaa tcccggaagc ggcagtgcgg acaactggta
    20281 tcgtaccttg tctgtatcag gcggaagcgg tgcaacagtg agtttcccta tccatggtgc
    20341 gggatttgca attattggac ctaacaacgc cgggacgaag ctggatgtct atgtagataa
    20401 caaccttgta gcggagaatg tttcgaccac agcagctgca agtcgttatg agtcttacag
    20461 ctatagtggt ctgagccttg cagatcataa tatcaaagta gtagtaaaga gtggaaccct
    20521 gaaaatagat gcactgtatg cgttgggtga gacaacccag atcggtgatg atgtgcttgt
    20581 cagcgtagaa acaaaatttc ctgcagctta tgcagttgag cagaatcaga ccctcaagaa
    20641 tttgccggct acggtagaag taaaaacagg aaatggaact gtagtgacca agcctatcgt
    20701 atggagcaat aatacggcgg aacatttctc ggaagcttat gcgacgacca gtgtcaccgg
    20761 tgtggtgcag gatggtgtca acagcttcgg agcaagtctc ggagtgacga ttccggtaga
    20821 agtggtgccg gagaatacgg tatattttat tgatatggta aagggaactc cggatgcaga
    20881 tgcaaaaacg gaagcctttg cagcagtgaa ggaactcaga ggaagtgcgc tgttgaacca
    20941 gactgcggat cagttaaaga cctccggcaa tagctggggg ctggtagata ctgacgcggg
    21001 aaccaagagt tacaccgata ctacagataa gacagcaacc ggtatttacg ggaacaataa
    21061 cgaatcaggc gaaactctga cctacgcgct gactttagat cccggtaaat acaccattac
    21121 ctctgcacac agagagtggt ggggaatgac cagaccgatg aacctgacag taacagtgga
    21181 taatattaca ctggatgccg gaacgattca ggtagacggc agcaatccga atgctgtaaa
    21241 tacttattct tttgacgtaa gaaccaaaca gaccgtaacc tatacgatca ctgcaaccgg
    21301 aacacaggct cctgtcatca gctggctcgt tgtcagtcgg accggagatg ccgaggaaat
    21361 tcagccgaat gacagtattc ccggtacatc aggcagtgtc atccgtgata cgaacggaaa
    21421 ggcgatgcag gcacacggcg gttccgcagc agccatgaaa gaaggaaccg gagagggatg
    21481 cgtcaatatt gacctcgatg gtgacggaca gatcacagag ggtaagaccg tatatctgtg
    21541 gtatggagaa gataagacca acaacaccag accggtagat ggtgtaaaat gttatgtttc
    21601 taccgatctt tacaactgga ccgacaaggg aacagtactg tatcttcaga gcagcatcct
    21661 gccgattgaa gaatccgcag agaaagcaat cacttcctcc gcaggagcaa atggaacagg
    21721 aacgacccag agttatcctg caatgcagct cagtaatacc aatttcgaga ctctgaaggc
    21781 atggggaaag ctgtccgcag caccagaggg tgtcaccgaa gcggaattcc gtgatgtcaa
    21841 gctgttctta agagcctatg tcacagagtt tgagaaagag ccaacgagcg ctgaggatat
    21901 atcctggatt gcaaagagtt atgatgagac aaaggtagag gcaggtagct tcctgtaccc
    21961 tgacagcaag accaatggaa cccagaccgt atccagatta cagcttgctt tcgagggaat
    22021 gtatggtaat tattgcatta ctgagcgacc taagatggtc tataatgaaa agacaaagaa
    22081 atttgtcatt attttccatg cagatggacc tttgtacaac aatgaaaagc tgtacaactg
    22141 ggtgaagaac ggtatgcagg gcaactgtga agccagcaga tacagccgcg ctatggttgg
    22201 ttttgcaaca agtgatacac cattcggacc tttcgaagtg gtaaatatga cccgtatgaa
    22261 ttatgatgag agcctgaatg cacagcggtt gggcgaagcc agagacatga ctgtgtttgt
    22321 cgatgatgtg gatgcaaatg cagacggagc aaaggatgct tatgtaatct actccagtga
    22381 gatgaatgca aagctgtatg catctctatt gaacagtgat tataccgggc tcgctgccaa
    22441 gggtaataca gcggacaatc aacagatggc agcccgtctg gtatctgaca atagcagaga
    22501 agcacccgct gtcatgaaat acgatggttg gtattacatg attacgagcg gaacggatgg
    22561 ctggaacagc accgcacata cctactatcg cagtcagaac attctaagcg gctgggaaaa
    22621 agtaggaaac ccggcaaaga atgataccgg aaaatgtttt gatacacagg tgacatatat
    22681 tattccgatt gatgcacctg caggaaagtt catttatatg ggagaccgtt ggaatggcaa
    22741 caaattgtcc gacagccgta ctgtctggct gcccttacag gtagatgcca cttcccatac
    22801 tattgcgatt ctgaatcgca ccaactggaa gacagaagaa ctggaagatc tgatcccggt
    22861 tggcatacag actgcacttc ccaagatcac atggacggac ggcagtaatt tgccggagaa
    22921 agtcacggta agctataagg gacagactgt ggagagtaag gttgcctggg acaagagttc
    22981 ttatcaggtc atcggcagaa ccactgtcac cggtaaactc atcgattgcc ggaacgcgga
    23041 gatctcaaca gaaatgctgg tatgtccgaa aaatgcagtt tattttgcaa atgcatcgaa
    23101 agcaccggta agtgcagatt atacttccat catgaaacag ctcggcaata cattgttaca
    23161 tacagtggat gtatatgatg gcgcctacag tacagaaaca ggatttggat atgtaggagc
    23221 agaaggaaag ctgcgtaaca gcacagatga catttatcaa agtatgcgtt atgcaacaga
    23281 caagacccag agcatcagtt atcggtttga tctggaagcc ggaaaatata atgtttatgt
    23341 gggtatgttt gacccctcca gctggtggga tggaaaacgc tatgcggata tcagtctgaa
    23401 tgataaggta gtaacagaga aatacaatta ccagaataat gtaaacgata ctcttacgta
    23461 tgaaaatgta gaggtaggcg aagaaggaac tctgacgata actatttcgc cgaatgcagc
    23521 tacatccagt gccgttcagg tcagctttat tgtagtggca aaagcgcaga aggatgcaga
    23581 agttaccccg actccggagc ctacggcaag tccaaagcct acggcaagtc caaagcctac
    23641 ggcagcacct acggcgacgc cctcaaacga ttccaatcat gaggatgaca aggacaacaa
    23701 cacctcgaat tctcctgtgg cagcaaccgc aactccggtt ccggtagcaa cagcaagacc
    23761 tgttcgtcct acagtgggtg caacaccaag tgatatggta aatgcgacac cgaccgttcc
    23821 ggcaacaaca cagaagacag aaaaaacagg agcaaccata atcaatgcag ccggtgaggc
    23881 tgaggcaatc ggatggaagg atatccttga aaatattcgt cagaatgtaa gtgatagcag
    23941 caataaagag atctatattg tggcaggcag cgacaccgtt gttcctgcag aagtattgca
    24001 aagcgtaatg ggaagcaacc tgtctctgag aatcgaaatc gaggaggaac tggcatggat
    24061 cattgaagga cgggagatca cagaaaatgt aggagatatt gatttcggaa tccttaggtc
    24121 agcggatcgg attcctgctg aactgataac acagacgaca cagggacatg attatattcc
    24181 tttgtcactg gtacacgacg gaagcttcgg catgaccgca aaactgtcag tccgtcttgg
    24241 tacagagaat atcggtaaat atgcaaatct cttctactat aaccctgaca atgcagcaat
    24301 ggagttagtt gcgttcggtc aggtggcaga cagcggatgg gttgaactca actatgagca
    24361 tgccagcgaa tatctgctgg ttatggctga cgagcctatg gatgtagaga atctgtccaa
    24421 ggatgcttcg tcggaagcag tttccgcaga aaatgacaac acagagagcc agaaccgtaa
    24481 tcacagcaaa gcaggaaaaa tttggattcc tattgtgatt gtaatagtat tgacaggatg
    24541 tgctgcagga tatttctatc tgcgtaagaa aaagtaaaag agtatatata ttctgtatga
    24601 tctaacatta aaaaccggcg aaatcttatt tgacatggct gtcattaaga tttcgtcggt
    24661 tttttgtata cggtaggtta tgcaggattt taaaatcacg agacatcatt gataagattg
    24721 ggttcagtag gatacagaaa gttgtattac tttgcggagt aaaacaagaa aagacagaaa
    24781 gaagtatctg caaataagaa gtatataaaa tgtttagatt tggacagtag aaataaaaac
    24841 gtttttatgc tatcatgcat ataacagtaa gaggtgatag ctatgaaaat agaacgaatt
    24901 gatgacaaga cagtaaagtg cttcctgtcc aatgaagaat tggaagagta cgagattgat
    24961 tataaagatt ttgtggtccg cagtgaaaaa gccaaagagg ttgtgcagga gattatcgag
    25021 caggccgagg aagaggtggg ctataagccg ccgaagtatg cttttgacct acagatcatg
    25081 atggtaccgg atgaaggatt gatgctggta ttttccgaga aggaaccgct ggagaataaa
    25141 aacggtgcac agctgttaca ggcgttgcag gagatgaagc gtgtcttcga ggagaaaaag
    25201 gcagagatcg gcgctacgct gggaatactg gcgaaggatg aaacggagat caattccggg
    25261 aaaaataaga aagcagaggg ttaagcagag gaagcggcaa a
//
LOCUS       DAWDHD010000020        25282 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_154_length_25282_cov_12.758275, whole genome shotgun sequence.
ACCESSION   DAWDHD010000020 DAWDHD010000000
VERSION     DAWDHD010000020.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 25282)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 25282)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..25282
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_154_length_25282_cov_12.758275"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    7672..7771
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5876 a         6871 c         5315 g         7120 t
ORIGIN      
        1 tcgcatgctc cgggagttcg tggattttgc caatgtattt aactaacttt caatttacaa
       61 tataattaca ttcgcgcatt tcacatcatt ggcgaggtcg tacactttgc cggtggtgag
      121 acccagaact ttggggcgca gcaccgcatt gggaacgttc tccacaatac gggcggtctc
      181 tccgttgctc aatgccacat ctgtgcctac cggatagaga ataacgctct ccaggaagca
      241 ccgcataact gtaatatcca gctcttccgt catggacatg atcatttcca cggcatctct
      301 gggagaaaac ggcttcttat acggccgctc cgtcaccaat gcatcataaa tatccgacac
      361 agagatcagt ctggcaaaca ggtcgatctt gtcccctgtg atccccatag gatatccttt
      421 tccgttcatt ttctcatgat gctgtaacac acccagcttg atctccatgg acagatcttc
      481 cttagactgc agaatacgat atccgtacac ggaatgctgc ttcataatgg caaactcttc
      541 atccgtcagc cgcgccgcct tattcaggat ctcattgggt acttttgatt ttccgatatc
      601 atgtaaaagt cctgcgattc cgatctcgta gacctgcttg tcatccagtc cgtatttctg
      661 cgccacgatc atggacatcg ttgccacatc cacactatgt ttgaaagtgt actcatcact
      721 gatcttcaat gcaccgatat ccactgctac tgcatcatta tcctcaatag cccgcagcag
      781 gtcatccgta atactccggg aagcattggt aaaatccggt gactgcgtat cctggtacaa
      841 atactgcact ccctgtgcca cgcggcttcg cacgctctct gagatctgta ccttggcagg
      901 gtccttcacc ttcacctgtt catatttttt ctgtaatggt tccggcagtt cctgcggctt
      961 gtccgcatcc gccggcttca catcctctgt accctcacag gtatagattc ctgtaacccc
     1021 cattttcttc agcgcatcga tatggaaatc ctccagtcgg gtacgtctgg cgatcagtac
     1081 acggcctgca cggtctatga ttgcctgatc gatcatcatc cccgactgca caatgcgcag
     1141 tggaacatat tttctcctga tcatcggtcc cttccccttt ccgaatgatt ttggtccatg
     1201 ctttattgta acatatcttc ataaattctt aaagatatct gtcaaaaagc ttaaaactgc
     1261 ttaaaagact ttttatggga caatccttta tactcttcta tagaaaagac ggttattttg
     1321 tcaaaaaggt tctaacggga gggatttcta tgaatacaaa tgcagcttat caggttccgg
     1381 tgcaacaacg ccgccgcaga atttccagac aggagatgct ccgcaggaag cgggcgatcc
     1441 gcagaagaaa acgcatagtc tgcttcctcc gggtctcatt tgccatcgtg ttgctcctga
     1501 ccgcagtctc tttctggcag gattacggcc atgagattct tcctgccgtc aatcatctgt
     1561 attatcagct gactgcccgg tatgatctgc cggttgtcca atatgatttt gctgcactgc
     1621 gggaagaaaa tgaatctgac gctgccggaa gtctctctac caccgcagat accgtagaca
     1681 tcacggaagc actggccgag cttgcccaaa ataatgccga agccgcggat ttcgtggcag
     1741 actaccccaa cagacaaaat tatctgggac agtccatcga tcttgcttcc gatgttctca
     1801 ttggacaggt gcctctgctc ctgcaatggg acaagcgttg gggctacgag tcctacggtg
     1861 atagcatcat cggtctcgcc ggctgtggtc ccacctgtct gtccatggtc tatgtctatt
     1921 tcacccacga tttgaacggc accccgagag agattgcctc ctactgtgaa aagaacggtt
     1981 attatacgga aaatggcacc agctggtccc tgtggaccga agggcttcct gccctcggcc
     2041 tgtccggcaa ggaacttccc ttgggtgaaa acagcatgaa aacagccctg gatcagggca
     2101 aacttatcgt atgcagcatg cggccggggg atttcaccac ctccggccac tatatcctgc
     2161 tctatgatta tgatgacacg ggcttttgcg tcaaagatcc caatcgggta agcaattctt
     2221 cccgcacctg ggattatgac acgctgtctc cgcagatcaa aaatctgtgg accatcagcc
     2281 ccactggttt ttaacaatgt ctgccagaat ctttgctgtg ccgtcgatgc cgaaccggct
     2341 gctgtcgatc atgagatcct tatggtcgaa aacgctctgc cagccgggaa tataggcttt
     2401 gtgataacgg tttctggcag catccacatc ccgcatcatt ttctttgccg tctggggatc
     2461 catctgcagg ttctccacac agtttttcag ccttgcctca tcgggggcat agacatacac
     2521 attcagatga ttcgggatat cctccagaat atagtccgca cagcgtccca caataatgca
     2581 ggatcccttg gctgcgaaat cccggatgat atttttctgg gtaagaaaga tctcatcctt
     2641 gaggctggac attcccattc cgagcggata ctgcctccgg aagaaagtag acttcatact
     2701 ctcttcttca tcgctgatga cagataccgg aaggcccatg cgcttcgccg tagcctccac
     2761 aatatctctg tccaggaatt ctattcccag tatttcggac agttccctgg caatggaacg
     2821 tcccatactt ccgaattgtc ttgaaatcgt gatcacatac tgttccatgg atatcctctt
     2881 ttcttttctc gtttaatctg cggtcacacg taccggtctc ttccgccgtt tctgcatggt
     2941 acgtaccacg caggacagtg tgtagttgat gataaaatag atcaatgcag ctacaccaaa
     3001 cagcacaaat acatccgata cgtttacatt gccagtacca ttgtaacggc tggctgcgga
     3061 aagcagctgt ttggtccggg ccatcagttc gatagtcgcc acatttgcca gataggaaga
     3121 gtccttgatg gtggtgatca cctgactcaa cagggtagga atgacattcc ggtaagcctg
     3181 cggcagaata atatagatca tgacctgtac cacattgaat ccctgtgcgt ggccggcttc
     3241 aaactgtcca tgtgcgatgg aattcagtcc tccgcggatg atctccgcaa taacggaaga
     3301 ggtaaacagg atcagagcaa ctgctgcctt gaacaacagc ttggtctcta ctgtggtcag
     3361 cccgaacagt ttccggttga acagctccgg gcaggggcag aataccaggc atatgaagat
     3421 ccacagcagc aacggtgtat ttcgaaatac ttctatgtat acagtggcaa tcatgccaaa
     3481 tattttcgtc tttttgcttg tgcagtagtt tctgcacagg gccagtaccg aaccgatgac
     3541 gattcccagc acaacagcga tcagggcaat gatcagagtg aataccgtac ctttgagaat
     3601 gtaggccata acacctttct gtcccagtat ggaaaagctt gcttccaggt tactcattgt
     3661 ccgtcaccgt cccttctgtt ctctgaaatg tcacccgctg gtctcttttc ttcagatgga
     3721 tctcccagac actggctgtc ttggagaggg gataacatat aacgaaaaat aacagtccac
     3781 tgaccagata ggccggagca taggctccac cggtattttc accggtgaca aagctgtaag
     3841 tcagcgagat cagatccgct ccgccgataa tatacaggca ggaggtattt ttgatcaggt
     3901 ttacgatctg gttcaccatg ggcggcagaa tgatcttgat gctctggggc aggataatat
     3961 aatacatccg ctccacataa gtgaatccct gggacacagc cgcctcgaac tgtcccttag
     4021 ggacggcctc gatgcccgcg cgcaccacct cggccatata ggctccggtg tatacgccca
     4081 gtgcaacgat acctgtcggc aggattccga tactgtgacc ggaaaaagcc agcgcatagt
     4141 agaagaaaca cagctgaagc aaaatcggtg tattctgcat gatctccaca tagactctgc
     4201 tgatgatctg caataccttt tttccgctgg tagccatcag gccaaagatc acacccagtg
     4261 ccagcgataa caggatcgca aaaaatgctg tctccacagt attcagaagt cctaacagga
     4321 atgtgctcct gtacgcccag accttgcccc aggctcttgc tgaaataata ccagacatag
     4381 tgtttcctcc cagttacttt tttctttttt actctaatcc gttctctgtg atcagactgt
     4441 caatggtacc gtctgccagc cagccctgta ccagttcatc cacgtatgca gagaagccgg
     4501 aacccttggt ggtagccacg ccatattcct gaggggagaa cttgtcgtcg atgtaggaac
     4561 gtccgtcagt cttgtaaata gccagaatgg acttatctac acagaaggca tctacttcac
     4621 cggcactcag tgcggtggag attgcagggt agtcatcata ctgctggaaa gagattccgg
     4681 tagtccacag tgcgggatca aaggtatctg catcaaagcc gctgccatcg atcaggcctg
     4741 catcgatcat agcctttacc agtgcctttg cggaagtgga tccggaggat acaccgacct
     4801 tcttgtctac caggtctgcc agggagctga tgccgctctt atcctctacc agtacggtta
     4861 catagtctgt aaaataagga gtggagaaat cccagctctg ctttctctca tcggtgatgg
     4921 tgaaagtagc cagtacacag tcaatatcgc cggaatccag aagttcggtt ctggtagctg
     4981 cggttacggc ggtgaactct acatccacgc caaggctctc tgcgatcttc tcagccaggc
     5041 tgatctccag tccggaatac tcaccggtca gggtatcctg gaagccaaag cctactactg
     5101 catttttaac gccgactctc aatacaccac ggtctacgat tgcctgtacg tctgctgcca
     5161 gctcgccgtc tgctgcagga gcttccgctc ctgtttctgt ggttgctgct gcggtacttt
     5221 ccgctgcagg tgctgttgcc tcggaagcgg gggcatcagc gctgccgcat ccggtaaata
     5281 aagttgcagt cattgccatg actgctgcca atgcagtcca ttttcttaga tttttcataa
     5341 cgttttcctc ctttaattcc ctttacttat atatctaaac acgaagcctt ctgactgaca
     5401 tgctgtaaat atgacttcgt ggatagacct gctgttatgt aatgctgcgg cctaacggat
     5461 gatcttgccc aggaactgtt ttacacgttc gttggaagga ttgtcgaaga aatgatccgg
     5521 cgtaccttcc tcaatgatct gtccgtctgc catgaatacg atacggtccg ccacctgtct
     5581 ggcaaatccc atctcgtggg taaccactac catggtgatg ttttccttag ccagcttgat
     5641 catgacatcc agtacttcct ggattgtctc cggatccagt gcggaagtcg gctcatcgaa
     5701 aagaatgatc ttcgtctgtg cacacagggc tctcgctatg gcgattctct gctgctgtcc
     5761 gccggaaagt gttgtgggat acacatgagc cttatcccta agtcctacca catccagata
     5821 atgataagcc agatcctcgg cttccttttt gctcttgtga tgcagcttca tgggtgctat
     5881 ggtcaggttc ttcaataccg tcatatgcgg atacaggttg aactgctgga ataccatgga
     5941 gatggactcc cggatgatct tggtcttgtt cgcatgggtc agcttctgtc catcaatata
     6001 tacttgacca ctggtaggct cctccaggaa attcatgcat cgcacggtgg tggatttacc
     6061 ggaaccggag ggtccgatga taaccagctt ctcgccttct tttacttcca gatttacatt
     6121 tttcagtacc tggagatctc cgaaattctt acatacattt tccagtctga tcattttgct
     6181 cctccattct acgttccggg caggaatttt tgataacaga aaaaggcgct tttaccgatc
     6241 cggtaaaaac gcctttgttt ttatgggtta catattatac agttttgtgc gctttgtcaa
     6301 gacttttatt tcattaattc tttcaggacc tcttttgcct tctccagctg attgtccacc
     6361 ggatcatcac tgttgtagta tgcttccccg tcaaactcgc attccacatc cggttcaata
     6421 ccggttccgt ggatattgcg gccattggga gtatagtaag cgctgatggt gatcttgatg
     6481 gcggatccgt cggtgaaggg catgatctgc tgtacgattc ctttaccgaa ggtcgtggta
     6541 cctaccaggg ttccaacgcc gtagtcctgg attgctccgg ccagaatctc ggaggcgctg
     6601 gcactgttca tatccaccag taccaccaga ggtacttcca gaggagtctt gccgtcacag
     6661 gtatattctg tgcgcttgcc tgccttgtcc tcggtatata cgatcatgcc ttccgggagc
     6721 agctttctgg caattttaac cactgcatcc agacttccac cgggattgcc ccgcagatcc
     6781 aggatcagac cttccatgcc ggagcccttc acggttgcca gcgcatcggt aaactggtct
     6841 atggtcacat cgtcgaattc cacgatctgg atatatgcca tattgtcatc caacatttcg
     6901 tattttacgg tgggactctc taccttggct ctctccacgg tgacgtgcag ataatccccg
     6961 gcgccctgtc tgacaatggt cagatccact gtggtcccgg cttctccgcg gatcatattc
     7021 accgcttccg tcaacgtctt acctgcagtg gagacaccgt ccacttcata gataatatcg
     7081 ttggcacgca gatccacatc agctgccggt gctccttcga tcacgccgct gatcttgggc
     7141 agattggtct cggaatccat gctaacatag gcaccgatgc caaagtaaat gccctgtgtc
     7201 tgttccgtca gttcattcaa ctcctctgcg gtatagtact cggaatacgg gtcatccaga
     7261 gagtccatca ggccacggaa cataccgtct tccagctgtt cattggtgac ctcactcaga
     7321 taaaagttct ccttcacagt gtcttccagc aattgcagtt ttttaatggt ctgtgcattg
     7381 accgcggaat cttctccata ggatacctgt gctgccacat catccttcat ggtcatgatg
     7441 ctgtggatct gaaaagcgat aaagataccg cccacgatca gcaacgtggc cgcaaagcca
     7501 acgataagtc cactgagaaa taagcctttt ttctcgccat tatttgccgc cggagttaca
     7561 gaagcctgtt cttcggtggt ggtttccagt tccttatttt cctccataaa tactctcctt
     7621 tatttccaag atattgtcgc aacacgtcgc gcttcttgtc gcaacacgca tnnnnnnnnn
     7681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nttctccgtg ctccgcactc ccgaaatgct
     7801 gcatacattc gcattccggg ctatcgacgc ttctccacat tggtttgtcg caacacgcat
     7861 ttctccgtgc tccgcactcc cgaaatgctg catacattcg cattccgggc tatcgcccta
     7921 cttgctcatg tatgtttgtt ctgctaatcg ccagagcact ttgcctgcaa gcagtcacgt
     7981 gccctgcgat tgcagaacta ttgcgacagg tagttccagg gggatgtgta agctccatcc
     8041 ttacgcacgc tgaagtgcag atggttgccg gtggaacgtc cggtggagcc cactgcggct
     8101 atcgtctctc ctctgaccac aatatcgtcc ttggatacat ataatgccga ggcatgcata
     8161 tagatcgtat acagtccacc accatggtcg agcatcacgt agttgcccat agtgttgctg
     8221 taagtggctg ccaccacctg tccgtcatag gccgcataaa tagctgtgcc cttgggcgcc
     8281 gcgaaatcca cgccgttgtg gaactgttcg attcccagtg tcggatggat acggttgccg
     8341 taatcatccg agatccgggt ataggttgcc agcgggaact taaaggtacc gccatcgtaa
     8401 gtcagcacag taccactggc ctccaggatc ttttttttct ctgcggcaat ggcagcctcc
     8461 agggctgcaa tctccgcatc ctgatccgca atggactgct tgtattcctt gatggcctgt
     8521 tccttgttgg taatatcgga ttcgtaagcg gtgatatccc ggttcttctg gtcgatcaga
     8581 gcctccatat tggcctgctc ctgttccacg ccggccttgg cctcatccag aatggctttc
     8641 tcctcttcca gttcctcttt gcagagttca atatatttgc ggttctccat gaagtctttc
     8701 cattgctgtc tgtcataagc catgatcaga tccatgaaat ccgcacggtt caggaaatca
     8761 ctgaagcttt ctgctttcaa catcatatcg aggatgtagg aatccccttt ctcatacata
     8821 attcttatgc ggcggatcat ggcatctttc tgattttctt ctctctccag tgccgcatcc
     8881 agttccgcct gggtctttgc aatttcttct tcttttgctg tgatcttatt tttcagatcc
     8941 gcaatattct gttcgatctg gctcagattc tgatccagct gggttacata atttttcaga
     9001 ttggtcttct gtgtctccag gtctttcttg atctgctcca aattgctcag attgcttttc
     9061 atctgttcac gttctttttc cgcctgtttg atctgatcct gtttgtcccg gatactgtca
     9121 gaggtaatgg atgacatatt ggtcgcattt acccgcaatc cctgcatgcc gaaggaaacc
     9181 atcaggatca ggcacagtgc caggcacagg aagccttttt tctttttatt caatttgatt
     9241 tagtctccat tcttaaaatg tcatgatgca gtgcagagcc acactccgca cacatcatga
     9301 caaataggat ccgggttatc tgctggaatt gtatttacac gtgcagatgc tttcttacag
     9361 tagcgatact tcccaggaaa ccgataccca caccgacacc cagagatacc gggatcagga
     9421 aatggaatat ctcgtccaca gacaggaaat tcaggaagcc gctgagtacc ataaaacggt
     9481 tgatcacata gttcaacgca taattgtaaa gggaatagat cagtcccagc ggtactgccg
     9541 caccgatcag accgatcagc ataccttcca gcacaaaggg cgcacgcacg aaaaagtcgg
     9601 tagcgccgat atatttcata atattgatct ccgcagaacg gacagaaata ccgatagcta
     9661 cggtattgct gatcaggaag atgctgactg ccagcaggat cacgatgatt cccatggaca
     9721 cgtaggcgat cagaaggttc agaccgctca gtgtgttggc tgtcatttcg gaatagttga
     9781 ccttgcgcac ctccggaatg gactgcagcc aggtcaccag tgcatcctgc atggagacat
     9841 cactcaggaa aatctcataa gtatcctctc ctgccagtgg attctcgggg aatccttctg
     9901 cataatcctc accgaaataa tccggtccga aggttgccca tgcatcatct gcagactgga
     9961 actgtacctc ggatacctct gcacgcttgg caatctcctg tccgatctct tccaggcgct
    10021 gttcggacgg gatctgtccg tctacatgac tctcacagtt atccatatca ctgtggaaaa
    10081 ataccgtaac ggatacacct tcttctgcag tcttcaggat attctgaaag ttggccacga
    10141 tgcaatagaa cagtccaaac agaaacagac atgcggatat cgtggcaatg gatgccagag
    10201 aataccattt attccggaaa atattggcaa atccctgctt gatcgtataa aaaagtgtac
    10261 taatcttcat cgatataccc acccttctct tcgtcactga tgatcacacc ttttttcatg
    10321 gtgatcactc gtttctgcat ggcattgacg atttcgcggt tatgggtgac cacgactacc
    10381 gtggtgccgc tctcattgat ctcctccagc aatttcatga tctcccagga attattggga
    10441 tccagattac cggtgggctc atctgccagc agtacggaag gattgttcac cagcgctctg
    10501 gccagtgcaa cacgctgctg ctcaccgccg gagagctgtt tggtcttggc cttatatttg
    10561 cctgccagtc ccacagttgc cagaatcgca ggaacatttc tgcggatctc actgacggga
    10621 gtctctacga tacgctgtgc gaatgctaca ttttcataga cattgcggtc attcagcagt
    10681 cggaaatcct ggaatacgac acccaggtta cgacgaaaac gggggatctg cctgtgcttt
    10741 aatttcgcca gatcatttcc cataacataa acactgccgc tggtcgctgt aagctcccgc
    10801 agcagtaatt tgatcaacgt ggatttaccg gatccgctgt cacctacaat aaatacaaat
    10861 tcccctcttc cgatgcttaa cgacacacca ttcagtgccg gagaacctga ggaataagac
    10921 ttgctgacgt tgtccaggaa aatgattccg ttttcctcaa tgatccgctc tcttttcagc
    10981 ctttgtgttt cttttcttgt cattttatga tcatgccttt ctattgcccg taagatccca
    11041 ccctgcggac agttgctgcc tttgtgctac catattgatc cctattcaga gcctcattcg
    11101 caaaatcgca tctacgatgc tcttctaaac aacaagttaa cttgttgttt gctcatttga
    11161 ggctttctcg ccaaagaaat aacgtcaaaa gttcttacga atgcaagcat tcgacgcaca
    11221 ctttccgtta tttcatggct ccgcaagaaa tcaatactca taagtttcca tatacttcat
    11281 gtattttacg accatcagtg cgatcttgaa ggtgatggca tcttcaaata cccgcagatc
    11341 cagccctgta ctcttctgta acttatccag acgatatacc agcgtatttc tgtgaataaa
    11401 cagctgtctg gaggtctctg acacattcag gctgttctcg aagaacttgt aaatggtcgt
    11461 cagggtctcc tcatcaaaat catccgggct cttgcctccg aagatctcac ggataaacat
    11521 tttgcacaga ggaatgggca gctgatagat cagacggccg attcccagtt cactgtatgc
    11581 gataatatct ctctcgtcaa agaagatctt gcctacatcc agagccatct ttgcttcctt
    11641 gtaggaacgg ctgacttctt tgatctcatg tacaatggta ccgtatgcaa tacggatctc
    11701 acggatacct tccttcagga gagccgcttc cagtgctctt gccgattttt caatttcttt
    11761 gccggtatcg gtctctgcga attctttcac cacgatcacg ttgctctcgt ctaccgctgt
    11821 gatgaaatcc ttgctgttgc tgccaaaatg agttctggcc agttccagga cattattatc
    11881 cttgcctccc gcagattcca cgatcatgac cactctgggc acgtctgtct gaatatgcag
    11941 cttcttggaa cggctgtaaa tatccaccag cagcagatta tccagcagca gattcttgat
    12001 gaaattgtcc ttatcaaatc tctccttata ggccaccagc agattctgga tctgaaacgc
    12061 aaccattttg ccgatcatat agacatcctc accggtacct ccggctacga gaatatactc
    12121 cagctgctgc tcatcataga tcttgaaata ctgatatccc tggatctcct gggagtccgc
    12181 cggtgatgcc gcgaattcca ccgcgggctt ggaacagttg cccatatcca tggtggctgc
    12241 tacttccttg ccatccacat ccatgacaca caattccact ctggcgattc ctttcaatcc
    12301 ttcaatggtg ttctgtaaaa tctgatttga tatcatcttc tgctccttca gtccccaaac
    12361 atacttctct tttgctataa ccccgaaaaa tgatatgaga gtccatatca tatataaatt
    12421 tacagaaaaa ctggtaaaaa tgcaatagtt ttttatgcat tttttagtaa aaatgccttt
    12481 ttcttttaaa tacgcgcaaa aaaagaattc ggaaaactaa tattccgaac tctttttgtc
    12541 ttagcgttta ttatgtatta tttctgcgct aaatccgcag atcgatattc gcaccaagtt
    12601 ccggtgttac gctgcgttcc atagcagcgg aatcaatcat ctggacgatt ccctgtccca
    12661 gtgcttcgtt cgtatccatc gccttgctca gcacagccgt tccgattttc gtctgcagat
    12721 tatttgaagc cattgacatt gataatcctg cgatatccat gataacacct cctgtatctg
    12781 ttcatatctc tcagattacg ttcttactat agcacacttt ttttgttttt tccacactat
    12841 cctcgcactc tgcgaatatt tttctccgtg atctggatct tgccttcttt taacagatgt
    12901 cctaccgcgc gcttgaactc gttcttgctc atgccggtct catgggcaat gacctccggc
    12961 gccgccttat cattaaacgg cagagaacct tcatagctgt ccagcagtct ctcgatcttc
    13021 tccgcatcct tctgaatctg cagataggct ttttcccgga cactcagtgt cagcttaccg
    13081 tcttccaaca cttttaccac tctggcagtg acctgatcgc caatcctgag ctcagtatct
    13141 ccgaacagtt cttttttcgg gatcagtccg gaaaagcagt tatccactgc cacaaacgca
    13201 ccgaaattct tgctgatctc atagatccgg ccggttacct tatcgtcttt tttgtacggg
    13261 ctgtccgtgc gcagatgttc atagacattc atggtcgcac acagtctgcc gctcttgtcg
    13321 atatagaggg ctgccagcac ctgttctccg gtctttacct tgcgggtctg ctgcttaaag
    13381 ggcaggagca gatccttctc cagtccccag tccaggaaag cacctacttt ccctacctgt
    13441 actacagtga gcagagccac ctgtcccata cacagcttcg gggttctcgt cgtagagatc
    13501 agacggtcac tggaatcccg gtacaggaat acttccagtt catctccgac ctttgctcct
    13561 tccggcacct gcttcgccgg cagcagcact ttgtcctccg gtgcttcctt cgttgccaga
    13621 tacactccga attctacttt tttcactatt accagcgtct gtttttcgcc taatctcaat
    13681 tccacgttct ctcctgttct gacagcatag gattactgtc cctgaaattc cactgataca
    13741 taaggtttcc cggcttcgtc ctgcaggcag atgacgtagt cattctctgt agacactaca
    13801 tacggatgca atacatcatc caggaatgcc tgtttcttgt attccgcccg catctgacgg
    13861 atcacaaaat catccggcag atactccatg gcaatattta catactgccc attgtttaca
    13921 tgatggttgg tgtccaggtg gtgttttttc accacgatgg gttccaacag ttctccacct
    13981 tccggcacgg caattttgcg gggggcatac tccatgtcca gcttttcgtc tatttcgtag
    14041 cccttatgca tggcttcgtt caccattacc ggtttgccgg tgccgacatc cagcagactc
    14101 cataaggagt tggctttcgc cagatattct ccctgttcat ccagcatggc aaaattacgg
    14161 tatcccaaaa atacttttag ctcatagggc agcgtcacga tctttacttt ttcccctaat
    14221 ttcggataac ggttcaccac gatctgccaa gaggaaagga cccagacctg accaatctcc
    14281 ttcatataac cgattcccag ccccacatcc tccgaatgga aggtggaaca atcctgaaaa
    14341 taattcagta atgcgtccag cgtaagatac ccttcactgt ctgtctcgct gtaacgtatt
    14401 ctactgtcaa atgcgtacat aacttccgat ctcctttgcg gcgatgttct gccacagccg
    14461 ttctttttac agatttcccg taattgccgt aacaatctcc gggaacatct ggataatgat
    14521 catggcgatc cagaaaatac agtagcatat cattcccgga ttgccgatca cggtcttgaa
    14581 tctccgtccc tttgcaatct gtcccggctc taatttcagc ttcttacgat atacaatgaa
    14641 caatacgatt cccgtaacca atactgccag aataaacaat acatagatca tatagccgat
    14701 ccagcccggc aggtacttca tcatgtagtc catgacagcc tgtgaatctg ccccgttcat
    14761 aatgacttcc tggtattcct ccagatggat tgctttcaat aacagtacac tgactacggc
    14821 acccataaaa ttaatgcaca tatgcaagcc gatggtcact ttcagttctc cggtcttcac
    14881 atacagaaat gccagaaaca ttcccagtaa aaatgcgtag gcaaactgat tcaggttgcc
    14941 atggaacaat ccgaacatca gaccggacag aacaacagct actccctgtc cgtattttac
    15001 ggtacgatcc acgatcagtt tcctgaaaat atattcctca aggatcggcg cgcagatcac
    15061 catatagaga aatgtaacga tcatattgga tccggtggca taggtcatca gtgcattgtc
    15121 cactgcggag ccttttaatg cccctacgac agtggtgatc agcgttccca ccagattacc
    15181 acaatacatc agtgcaaaac acatgatcag tgccagaaca aactgtcccg gtgtaatgct
    15241 tttcttcgcg ggtgcttccc cgggcatccg ctttaccaca gcgatcagaa ccggcatacc
    15301 cacaagatac agtggtaata ctgccaagat caggcttatg gtggtgtcct gcagccattc
    15361 cggcttcacc gcacctacga tggtcattac tcccatctgc actgcgtaga tgaccagtgt
    15421 tccgatcagg aatcggaaac ctaagctgga aaaacttttc ttcatgcttt tttcttccat
    15481 ttaattttct ctccctcatg tactataaac ttttcagaat cacattttct aaatccgctc
    15541 tgaatagtta cagcatagca taaatccttt gtgtatgaaa ctattttttc catggcttaa
    15601 gacggttcca taggaaaagg ctgcgcatct tcaatatgca cagcctctgt aatcttcctc
    15661 tatgacgatc ctgttattct cccagaccgt tctctacggc ttctaccagg gtcttcaggg
    15721 cttctgcctc atcctcaccc tcacatacga attcgatctc atccccacat tttacgcaag
    15781 cacccaatac actgagtacg ctcttggcat ttgcagtatt ttcacgaaat ttgaaggtga
    15841 tcaatgactt gaactgcatt gcctccttgc acagaattcc ggcgggacgc agatgcaggc
    15901 ccgtgggatt cttgattacg actttctgac ttaccatata ataaaccctc caatatctat
    15961 attattctgc attattttct ctagtgttta tgatcttact tttcatttac atttctatca
    16021 tagcatgaat gaaaagcatt cacaagggct tttaccagac aactttcgga atctgtctgc
    16081 tagaacatga tatcctgtat caggttggca agcttccgtg cttcttcctg gatcacctgc
    16141 tgatcctttc cttcgatcat gactcttacc agcggttcgg ttccggaagg ccggatcagg
    16201 acacggccct ctccggcgaa tttttcttcc agtttcccaa tggctgctgc gatctccgga
    16261 tattccatat atttctcttt tttgtgattg gccactttgg catttaccaa tgcctgaggc
    16321 agtacttcca tcaccttagc caattctgac agaggctttc cggtatccac catgacctgc
    16381 agcagatgca gtgcactgag tagtccgtct ccggtggtat tctcatccag gaaaataatg
    16441 tgaccggact gttcgccgcc gaggctggcg ccgatctcct tcatgcgctc cagtacatag
    16501 cggtcaccta ccttggtctt ctccatatgg atggcattct tctctgccat caggaagaat
    16561 cccagattac tcataacggt tgccacgatg gtatccttct tcagagtacc cttgctcttc
    16621 atgtagttgc ctacgattgc catgatctgg tcaccatcga cgatattacc gttctcatcc
    16681 acagccagca gtctgtctgc gtctccatcg aaggccagac cgacattggc cttttcatag
    16741 accactctgg cctgcagttc ttccatatgg gtggaaccac agttggcatt gatattggta
    16801 ccgtccggat tgttgtgaat ggccaccacg ctgccgccca gttctttcag gcattctacg
    16861 gatgtataga aagaggcacc ctctgcacaa tccaccacga tcttcagtcc ggtcagatcc
    16921 acattgaccg ctctcatggc atgattgatg tactcttctc tggcatcggt acggtacttg
    16981 atcttgccga ctccgggacc gatgggaaat ttgataccgg gcatattgtt acggatcaat
    17041 gcctcgatct catcttccat ggcatcggga agcttatagc cgttaccgtc aaagaattta
    17101 ataccattga actctaccgg attgtgggat gcggagatca caacgccggc atctactttg
    17161 tatttcttgg tcagataagc aaccgcagga gtgggaatca cacctacgta gacacagttg
    17221 gctcctacgg agcaggctcc tgccatcaac gcattcgcca gcatatctcc ggaaattctg
    17281 gtatcacaac ccaccatgat cgtgggtttg tgctcatttt ccttcgtaag tacataggca
    17341 cctgcctgcc ccagttgcat ggcaagcaac ggagtcagtt cctcgttggc aattcctcgt
    17401 acaccatctg ttccaaacat tctagccatg ataaaaaacc tctcattcta tcttttaatt
    17461 atttgtatcc tgattcttca gtcggatgtg gatattgaca tcattttcta ccatgatatc
    17521 ctccgacagg ttaaacgtca cagggatcgt gtaatttccc ggtgtcagtt cttctacatt
    17581 gttgtcatcc atccatttct ccagatccag tactcccgtc accgtatcat cccgcagaat
    17641 actgatatct ctgtccaggc cggataccgt gatatcataa gtctccgcct ctgtagtgat
    17701 ctctgcctcc attccctccg gcacaccgct gatcgtaatg ttctctgccg gtaaggacag
    17761 gtccttggtc gcgatcggtt caatgtatac cgttgcagtg atcttgccgt caaagctctt
    17821 atccgccagt ctgacgttag cgggcaggta ctccttcaga ttaatgatat ccaccagatc
    17881 gctggactgt ccggtaatat tcatacgatc ctccggtacg gtaatggcag atactcccaa
    17941 cagtgctgat gccgttccgg cgatcttcac tgtagacctg ctactctcta cttcaccggt
    18001 agccatataa ccgtcctcag gtacacccat atattcaatc tctaccggta cttccttcgt
    18061 tgccagcact tctactgtca tatgcgcaga atccacgttt ttcttgacag tttccagaga
    18121 tagttcattg tcgtccgcat catacagctt aatgtccaca ttagccgaca gactgctggt
    18181 ggaatctgct acattgatgt ccactccggc atgatcgatc tgtgagatcg ctgatttagg
    18241 tccggttact tcgatgttgg tctgatccaa cgttacattg ccgatcatat aaccggatgc
    18301 cacctctccc agcgtggtgc tctccagttt gatccatttg ctggccttgt cttccacatt
    18361 caggcgcacg gaatcgtgat tacccctgat ctccacggaa tcgttgctga tattctcaca
    18421 ataataagtg atggcgatcg tattgatatc tgtcagtttg ctcatatccg cttccgctac
    18481 aatatcgttt ctgcgcaatt cggattttac aatactctgg gggcctgtta ccgttactct
    18541 taccagatta ctgttgtcca gcacctcata tacttttcct tcttgctcca gcagatctgt
    18601 gttaatcagt ctgacctgga tattattgaa ggttaccgtg tccttcggat catagatctg
    18661 tgccaccaga aaccacaata caaaggccag caccagagaa gcaagcttca gtccaagatt
    18721 acgtgtcagt aattttttca tttcttctca cggctccttc ccttccagat tcgcagattt
    18781 ttgtgttcct ctccggtatt gttcaggatc ttatgcattc tgtcgcgaag agtctccgca
    18841 tccagattcc gctccagctt tccttcgtat gcaacgctga tcttacccgt ctcttcggat
    18901 acgatcaccg tcagggaatc tgtcacctcc gagataccca caccggcacg atgtctggta
    18961 cccagttcct tgctcagtcc caggttatcc gacagcggca gataacaggt ggcagaggct
    19021 acccggttcc cctgtacgat cacagcaccg tcatgcagcg gggtgttgtg ctcgaaaata
    19081 ttgatcagca gctggctggt cacgatacca tccacatcga ttcccgttct ctcataatcc
    19141 cgcagggaaa ccttctgttc gataacgatc agggcaccgg tcttgacctt gcccatctcc
    19201 acacaggctt tggtgatctc attaatggtc ttctcggaaa atccctcgtc cacacgatga
    19261 ttgttgtcaa aaggtaatac agaactgata atattcttct tgcccagttc ctccaatgcc
    19321 tttctcaatt ccggctgtag caccactatg agggctgtca ccgcaaatcc cagaacattc
    19381 tgggccatcc acaggatggt agtcatacgg aaaaaataag ccaacagcaa aaaaatgagg
    19441 atgacaacca gacccttgag caatacccat gcccgggtcg tcttcatcca cactaacgca
    19501 taatacagta gtaccgcaat gatcaggatc tcggccacat cgccgaaatc catggttgtg
    19561 cggtaactgc tgaaattttt aatatattct gccagcttat cccctaattg cattttccat
    19621 taatcccttc agaacaattc ttcataatca tcggagtcat cctgaggatt cgtgtgattg
    19681 ccgcctatag tgacacttct gccaccggac atctcgtgtc cgcggtaacc gccctgctgt
    19741 ccgtaaggaa ctccgtttct ggcgggggta cgttctgtca caacacttcg gcctgttcct
    19801 tctgagggcc tgccggtatg tgccgtactt ctgtaggtct ggcctgcaga tctcgtaccc
    19861 tgtccggagg ttctggtctg ctgacctgcc ggacgtcgct gtgtattgat tttcttcact
    19921 gtattggtgg gcgccgcaac tgtcattttg ggcactgcct ttacatagta ataatactcg
    19981 tcatcttcaa actgtacttt ctccgttctg ctatagtcca gacagaatcg gaaaaattct
    20041 atgatcttgc aggcgatcag cgtaacaacg gcacctaaca gtgcgctgac gatggagaga
    20101 ttggtatcgt acatcagatc tcccaccagc aggatcatca cttcgatcat aacgccggca
    20161 accatggcaa tggtccagga gtgatccaca ctcattctcc ggatcagata caccacgatc
    20221 acggtaatgg caaatgctgc gatcgtcacc agcattgcct tgttccccag cattccgtca
    20281 atcagcaggc gcagtttggc agtggcttcc tctgctccca tggagttgat ggtcggagca
    20341 ttggtgatca ccgtctggag cagataatac accacaacac cgcagcctac agatacgcag
    20401 gacgccgggg ttccgatcag tcccattgcc acaggcatca catagggcag cttcagcata
    20461 caaagcaccg gggtcagtac cactaccagt gcttccttcg cggtaaatct gagaaacagc
    20521 agatacagca acaggaaaac aaccagccca accgccgcgg tttcaataga gagtgcatac
    20581 atatgaagca ctgcaaacat cattgcaaaa aaagccataa atccggtggg caggaaagaa
    20641 cacagcaacg atacgatcag cacgatggcc atgttatcca gctttgtcat atatcccatt
    20701 ttgccgttta tgatgctcag cacgataaag ctgagtaaaa acttgatcac cggcagaata
    20761 aaagcttcat ttctgctgta gatcttcttg agattctctc ttaattccag taaagcagtc
    20821 atatttcctc caggtcaatc tgtgtaggcc gtttactcat tgtaaatgga attcaacttc
    20881 ttcagattgt tataatagca tttcaggctc tttttcgccc gtgcatagcg cagagagcag
    20941 atcagataag atgccgctcc atacactaca accgttattc catagtacag cagaacattt
    21001 tttccaaaac cgatcaggtc catcttgtaa atatcctgca tgaaattctc aaaatcatag
    21061 aacacaaaca acgcaaagca gactccatac gcgatcgtcg cgcacagaat ggacttcaac
    21121 acctgtatgg caatgtagtc actgcggaaa taacggccga tggccatatt ctttttgcct
    21181 tcattctctt cataagatgc catttgcgtc atcagtatga ctttttcttc gttcagcata
    21241 attcctccgc ttacaaatgc ttacagataa cgcattcttt ctccgtctct ggatgttctg
    21301 gggctctgct ggggcgcagt ctgcttcttc ggcagtacac tctgcagatc catggccata
    21361 tcatatttgc acttttcgca aaatcttccg ctgcggatcg gtgctccgca tttctcacag
    21421 ttcagcagaa tactggatcc ttctgtcagt tccagtctct cttctctcag ccactgctgg
    21481 atctgggacg gctccacgtc acagttctcc gatacatcct gaatacttac accggggtta
    21541 ttacggatgt attcctttac ctcctggaac ttggcttcca tcttctcacg gcaatgcggg
    21601 cacagaaacg gtcctgccac atagttaaaa atatgtccgc acattctgca atttcttata
    21661 ttcatagtat ctcacacctt cctgatcata caccagaccc acaggccaat gtcacgcaat
    21721 acacttttga cactccgtgc tcttttaaca gtgctgcgat ctcatccagg gtcgtacctg
    21781 ttgtataaat atcatccacc agaataatcc ggtcatataa ttttacatca tttctgccta
    21841 taataaaagc ctttttcaaa ttattttgcc gttctgtggg atttaaatgc ttcatcggtg
    21901 ctgtatttct cacccgtttt atcagtttct catctaccgg cagttccagg cttctgccca
    21961 aagcccttgc cagacatgct gcctggttat atcctctttt ccgcagtttc ccacggtaca
    22021 gcggcaccgg aactataacg tccggacgca cccggccgat aaaatctccc aacagacgcg
    22081 ccatttcttc tccgaaaaaa tcgccgtatt cctgccgtcc actatactta aaacgataga
    22141 ttgacggagc cacatccctg tattcgtata aagttctggc tctgtcgtat ttatgttccc
    22201 ggtgcataca atcaccgcaa tactcccgtt cctccgccag tttctttccg caacgcatac
    22261 accacggagc cgtcaccggc tttaatttag gcagacattc cgggcagatt ttcctgccaa
    22321 aaggacggac gatcccgtca cagaccgggc atcttccggg aaacagcagc tgtaatcccg
    22381 tttcctttat tttgcttatg gtttttctga tctctttctg caatctcagg ctcccgtttt
    22441 gttttccgtc tacggtgtat cattttccgg caggcagcag atctcccgga tccgctgctc
    22501 caatcccgta tatctgcgat tctcgctgac attctcgatc atgttccgca cggtctcact
    22561 gcttcccaga atggtgacac agttcctggc acgggtaatt ccggtgtaca gaaggttgcg
    22621 gttgaacagc attctggggc cgcccaacag tggcaggatc accgcaggat attcgcttcc
    22681 ctgggactta tggatcgtaa tggcataaga gagttccagt tcctccaatg ccgagaaagg
    22741 atagttgacc cgtcttccgt catcgaattc caccagcagt gtggacatgg tatcattgat
    22801 ttccttaacc cttcccatat ctccgttgaa tacgcccatt cccttgtcga tgggaatatt
    22861 gtatctcccg acgatctccc attcgatctg gtaattgttt ttgatctgca tgaccttatc
    22921 gccttcccgg aaaacggtat tgccataggc atgctccttt ttatgaggat ccgctggatt
    22981 cagataacgc tgtaagatct cattcaacgt ctcacagccc agacttccct tcctcatagg
    23041 ggtcagtacc tgaatatcaa aggaggtggc tcccacatat ctgggcagca tatcctgaat
    23101 cagctggatc atgtgcttat atatgacatt tacgtcattt ctttccagta aaaagaaatc
    23161 ctttgactta ttgtccagag tgatctgctc tcctctgtta atccgatggg cattgacgac
    23221 aatgtcactt tgtccggcct gccggaagat cttctccaga gttaccgtgg agaacgcccg
    23281 gctgttcatg aggtcccgca atacctgtcc gggtcccaca ctgggcagct gatccacatc
    23341 tcccaccagg atcagtcgtg ttcccggaag aattgctttc agaagcgcct gaaacagttg
    23401 aatatccacc atggacattt catcaatgat caccacatcc gcctctaagg ggttctcctg
    23461 gttccgctcg aaatttgcct ttctggtgtc gtcttccgac agggcactgt tcagttccaa
    23521 cagacgatga atggtcttcg cctcaaatcc ggtggcttcc gtcattctct tggctgctct
    23581 gcccgtaggt gctgccagaa aaatgtccat gccttccgat tcaaagaatc ggatgatcat
    23641 attaatggtg gtggtttttc cggtgccggg acctccggac aggataaaca ggccattttt
    23701 gatactttcc tccaccgctt tcagctgcag ttcatccagt tccatggaca gattttccgc
    23761 catgcgttcc agacgtttgc ggatcgctgc atcctgggag ggaagatttt ctgcctccac
    23821 catagggata ttcagattcc tgagcatatt ggcacaattc aactccgcat agtaataaga
    23881 ggaagcaaat actgcttctc cctttgccac aagctttcgg tccatgataa ggttatccac
    23941 ctgaggacgg atattttcct ctgatacccc caatagttcg gaagctctgc gcagcagaag
    24001 atccaacggc agataacaat gaccttctcc caccgcctgc agcagggtat acagaatgcc
    24061 actgcggatg cggtaatccg agtcggtatg aatgccgatc ttccctgcga tctcgtccgc
    24121 gatacggaat cccacaccac tgacatcctc tgccaggcga tagggattct cccgcataac
    24181 gctgtacatt tccataccat aggtattata tatttttacg gcaagagtat tactgattcc
    24241 gtattgctgt aaatagacca gtgcttcccg gagatccttt ttttcttcca tctggatagc
    24301 gatctcctgt gccttgcgtt cgctgatccc ctttacctca gccagacgct ccggctcctc
    24361 ctcgatgatg cggaaagtat cgtctccgaa tttttttacg attctggctg ccagtgcctc
    24421 accgactccc cggattgctc cggaacccag ataccgttcc attcccacgc ggtcggtcgg
    24481 cgttacggtc tcgtagctct ggatcttgaa ctgtcggccg tatacgggat gttctatgta
    24541 ttctccctct gcggaaatat tttctccctg ggtcagtcct ttgcacatac cgacacaggt
    24601 catctcttcc ccttctgtcg acaggatcat taccgtatat ccgttttcac tgttttgaaa
    24661 cacaatatgg tctacaaaac ctgtaatcgt ctccattttt attcctcata ctatctgtct
    24721 gttgatttgt gggtttcaag gctgttctct ccgaattggg aagaggtcac cgcggtggcg
    24781 gcgtaagata aaaaagatca gggctgccgg aaatggcagc ccgaagtcac tactcaagat
    24841 tgtaattacg cttcatctgc tcatatagca tctgtgccaa tcccagactg ttctgctccg
    24901 tagagtcgga tgcgatctgc tgtaccatct catccttata gtaatccata agctgggagt
    24961 tggaagaaga agtctcatcg ctctcaggga tggtcttcat catttccttg aacatctgct
    25021 ccaggaaata agcctcgaac tgcttgcagg catccatcag ttcatcgtct gttgctttgg
    25081 tgtaatccga accgtttaac tggttctgca atttggaagc cgtctggttc gccgcattgg
    25141 tgtatgcggt gctgtccaga gcggaactgc cgtatataga agagatatcc ataaatgggc
    25201 ctccttagtt tttaggtttt ctatgctcta cggtaatgag caatgcatct cgtaaacgct
    25261 tcgcgtttcc ttcgatgtcg gg
//
LOCUS       DAWDHD010000021        24396 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_159_length_24396_cov_10.283144, whole genome shotgun sequence.
ACCESSION   DAWDHD010000021 DAWDHD010000000
VERSION     DAWDHD010000021.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 24396)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 24396)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..24396
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_159_length_24396_cov_10.283144"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         5437 a         6933 c         5158 g         6868 t
ORIGIN      
        1 ccggctccgg agaatccgga gatcaccatc aatattccct tattctgcat cttctctgtc
       61 ctctttttcc tcttccggcc acagtgcctg tgctcttccc gcgattgtct cgggaagcag
      121 tgccgacaga acgacctgac tgttctccat gaccagtacc gctttcgtct tccgtccctg
      181 tgtggcgtcg attgcctgtc cggtctcctt ggccttctgc accagtcgtt tgatgggtgc
      241 ggcctccggg ctgacgatgg cgatgatctt atccgtattt acaatattgc caaatcctat
      301 atggatcaat cggttcatgg tatcaccttt cgtggctatt caatattttg tatttgttca
      361 cgaacctttt cgatctcggt cttgagctca atggcacagt tggagatctc cagatcgttg
      421 gacttggaca ggatggtgtt ggcttcgcgg ttcatctcct gtgccagaaa atccagcttt
      481 cttccgatac tgccaccggc catcagcgta ctcttcgtgg tctcgatatg gctgcgcagt
      541 ctcaccagtt cctcatctac gcatacctta tctgcgaaga tcgtcacctc ggtcagaagt
      601 cttccttcat ctacagtagt atcgcccagc agttccttca cacggtcttc cagcttctgg
      661 cggtattcag caatgatctg gggagaacgt tccgtaatga aatccaccag cttcagcatg
      721 ccatccagct tgctaattaa gtcatctctt aaatgctcgc cctcggtgat cctggtctgt
      781 acaaacatct cgcaggcacc cttcagggct ctctgcagtt ctttccacag ttcttcctca
      841 tcgatgtcct gttcttccat cacaaataca tccggatact tggacaaagt ggacactcgg
      901 atatcgcagt ctaagtcaaa atcactggcc atctgcttca ggtatcccag atactccgcc
      961 gccagttcct tgttgtaacg gacattggac gtgttctcgg aaaaatcctc gtaggtaatg
     1021 aacagatcta ctttacctct ggaaatataa ttcttcagtt cactgcggat cgcagattca
     1081 aagaaattca acttcttcgg cattttgatg ttcacatcaa ggtagcgatg attcacagac
     1141 ttcatctcta cggtaaactt tctgttattc tcggcagttt cacatctgcc aaatcccgtc
     1201 atactcttga tcatagcttc tcctgttgta agggcgtgta ataccttccg caatttacgg
     1261 ttttcattct attatagaat aaatatgccc tttccgtcaa gtgcaggtgc cctatttaca
     1321 ctatttccac tcgtcgtaat gctccagaaa atgatgcttc acaccatcct ctttgtaggc
     1381 gatgatcgta cgtaagttta cattctccac actcttgaaa atattcccct cgataaaagg
     1441 gttagtctgt ttcagcgaag cgatcaggtt cttaatctcc atacgcttgg agggactgct
     1501 gccatcggta cgacaagtgg tacaagtgtc agtgaaccgg caggcacact gaataaaatg
     1561 cagatcattg taatctctcc agtgcttgat gtcatcctcc ctgatcaggt acatagggcg
     1621 gatcagctcc atgccctcga aattcgtact gtgaagcttc ggcatcatgg tctgtacctg
     1681 tccaccgtac agcattccca tcaggatcgt ctcgatgacg tcatcgtaat gatgtcccag
     1741 agcgatcttg ttgcagccca gctctttcgc atgactgtac agatagcctc tgcgcattct
     1801 cgcacacaga tagcaggggg aatcctctat gtcatacacc gcatcaaata tctgggattc
     1861 aaaaatggtg atgggaatat ccatcagctt cgcattgttc tcaatgatct tacggtttgc
     1921 ctcgctgtag ccgggatcca tcactaaaaa taccagttca aaggggaact ggttatggcg
     1981 tttcagctcc tggaacagtt ttgccatcag catggaatct ttaccgccgg aaatgcatac
     2041 agcgatcttg tcgttttcct gaatcatatc gtattcgttg atagctttgg caaaccggga
     2101 aaacaggact ttgtggaact ttttgcggat gctcttctca atttcccttg cccgttcatt
     2161 ttccttttct ccgggctgtt ccttctgcat caggttcagc agccatccgg tatagccgcc
     2221 ctgtaagctg taggcctcca ttccctgttc tctgaaatac tctgcagaca ccttactgtt
     2281 ctgtccacgg ctgcaataca aaattacttt tttatcccct gctgccgccg caagtctgtc
     2341 tataagttca cattcgggga ttgcaatggc tccgggaatc attccatagt ttactgcttc
     2401 atcccctctg gtatccacca ggatgtatcg gtcaaggtcc cgggactgta gttcttccca
     2461 tgtgatctca atgggccgta ttgtctcttt ttcttccatt tttatattcc tttactctgc
     2521 ccgaacaggc agtctattgt ttttcctctc tcagtctgct acactgttat taaaaatatg
     2581 gtaactgaat cgttacatta catccataga aaggcacaca aatgattcct tatactactt
     2641 tagattccat tatagctcaa atgcccgaac gcaccgggca cactctgtta gatgaagacc
     2701 aggggtgtgt aaaagggtgt cgtatgggcg tatccctcta tcgcaacaca gaatatcaca
     2761 tgccgcaaaa gcatgacgat caggaaggct tcttcgtatt ggacggaaaa ggatatgctc
     2821 tcatcgatgg cgaagaaatc cctctgctgc ccggtaccgc ctttatggta ccggctcaca
     2881 cggatcatgt gatcagacgg gatccctcct gtgactgctg taaggttcta tggttccatg
     2941 ccgcagtaga atgatcctca gtccccacat accgccagaa gctccgtcag ctgcgcctga
     3001 agctctgtca ggctgtcaca ctttttgtct accagcgcaa gaatctcttc tgtgggcggc
     3061 agctggtccg gcaccatcac cgtatataag cctgccgcat aggcggaccg gataccgtta
     3121 aaggaatctt ccaccgccag tgcctgtccg ggtgcaaccc caagctcctc gcatgccttc
     3181 agataaatat cgggtgcagg cttgccatgc tccaccatgt cgccgcagat aattttctga
     3241 aaataagatg tgatacccgc tctgtccaga tgctccataa ctgcggtcca gcgtgtagag
     3301 gatgccaatg ctgttggaat gcggttctcc ttcagatatt ccaggatctc atgcacgccc
     3361 ggcttctcgg gaaggccttc ccgttcttcc ttttccttaa atctccggct gcaatcctca
     3421 tgcaattctt caaaagacac atcgatgccc tgctctgctt tcgctgcctc gaacacttcc
     3481 ttcgtgccct gcagtgtcct tccgatacag gctttggaaa tctcgtccac atttttaagg
     3541 cccctgggct ccgccgcttc tctccagcac tccatataca tcctctcggt atcaaagagg
     3601 acaccgtcca tatcaaatat cactgcggtt attgttgtct tcataattct cctcacatcc
     3661 agagcaccat cagattgtta cccataccga tgatccccat cattttgcgc gtaaatagta
     3721 atcgatgata tctgtctctt ccatggaaat ataagttccg tacatggcta tgttgcagca
     3781 caataccctg cttcccgcag ggaagatcat ctcatatcct ttggacgaag ccagccccgg
     3841 atatctgtca tccgctcttc cgtaggaaaa ataagcagcg gaattaccta tcttcaaagg
     3901 tttcggtgcc ccaatccctc cggcgatcag agcctcgtcc tttttccatt caaaaaagct
     3961 ccagttcttt ttctctccga gttgccttgg ttctctgctt cgttccgtca gcagaatctt
     4021 accctgtgta tttaaaaatg tcaatgctcc cgtcttttta tccacacgga tctgggcata
     4081 tcccgctgta atttccacca gggaaggatt ctcccgcacc gtgcatctga ggtctcctgc
     4141 tgccgtcacc gccgctttcg ggaattcacg acactgccct ttggcaaaac aaatccggaa
     4201 cagatccacc gcaaggggtg tgatccgcaa ggttccgtaa ctactcaaaa gatccacata
     4261 gcccttgccc ttcatgacca tgcgcacagc gcagtcgatc ctggaatgtc cggcgggcat
     4321 cagtgctttg gcatccggca tatcgccgta ctcgccgtta ttttctacca tccgcggctc
     4381 ctgtctgccg gttgctctgc cagcattgcc gtttcctgcc ccgtatactt tcgcaggtga
     4441 cggctgtccc gtccgcctcg attcaggtcc ccctgacttt cctgctttct tcggtgtagc
     4501 gccgtctgtt gtcccctgac tgtttcttgt cacgatgatc ttctttgggc cgtaacgctc
     4561 tctggccaca cgttcctttt ccgcttcctg tgccctctgt ctgtagatct cttcttttgt
     4621 cttctccggc tctgcctgtt ttaccaccgt tttcaccggc ttcttctggg catctgcggc
     4681 aaggcgcatt ctctgcttct gctctgggga taccggatct gcgatcagtc cggtgagttt
     4741 cccccggtac agcaccgtca gttcatgcag ggctcgggtg gctgccacgt acagctgctt
     4801 cacatacccg tcttccaccg ggtaatcttc ctccgaagcg ttgaagatca gtactgcatc
     4861 gaattccaga cctttggtgt attttaacgg aagtaccatg acgccctcgc cgatctccca
     4921 ttttcccgca tctccggtat tcagttccac gccgtcctgt aaggctgctg ttactttttc
     4981 cgcctccgcc tcgtcaatgc agactacggc aatggtatcc agccccttgc tctgccatgc
     5041 tttgatgatc tgctccgcct gtgtcacctg ctctgtaaaa tccacacatt ttttcaccgt
     5101 gaccggttcc ccatgacgta tgatgggctc caccggatag accggaaagc ttccgtgata
     5161 cagaatgtcc gtggcaaact ccgagatttc caccgtgtta cggtagcttt tctgcaaaat
     5221 gccgaaataa tcaaactctc caggcagcat caatttccgc agttccgtcc agtcgttgag
     5281 accgtagcgg tcggaaatat tctgggccac atctcccatg atcgtatagg tacatttgct
     5341 cagacaatat tttaaagacg cataggccat catgccgaaa tcctgggctt catcaatcac
     5401 cacatgcccc gcctcccgga tcaccgtgtc ctccttgagg cgcttgtaga ggtaggccat
     5461 ggccgccaga tcatagacat cgtagccacc ttccggcacc tcgaccgtga aatctttctc
     5521 ctgctcttcc ttcaggaact gttcgtagag ttccgccaca gagcccttcc attccctttt
     5581 tccgaaatag gtctcataat aatgcttgag cttctgcttt tccggctggg tataggaata
     5641 atattttccg ctcagctcat tttccagccg tgccatcaga taatccgtca gtctgctgat
     5701 tttgtccgcc cgggacagat cctttgtctc tttcaataag cgtgcaatgg tggcccggtc
     5761 cagcagcact ttgccagtct tttcgatcac cacgtcctct ctgggtatcg cgcggtattc
     5821 ataacgcagg cagaaattct ccagctcctt aaaccagaca agagttccct tcaccgccgg
     5881 ggtcacacct tttaccaccg gcttgatctg ccaggacttg tcccagtcct cgtacaaaag
     5941 ccttacgaac aactgctcca tggtcatctg ggagacaccg taaacattca gttccggcag
     6001 cactcccgtt atatagttta agagcacctg attgcttccc acaatataaa aatcttccgg
     6061 tgcaaactcc tgctcgtagt tgtacaggat gtaggaaata cggtgcatgg ccactgtggt
     6121 cttgccggag cccgccgctc cctgtacgat catgttgtgc tggggctttt tgcggatgac
     6181 ttcgttctgc tcctgctgga ttgtggcaat gatctcgccc agcaccgcct ttttgttccg
     6241 cgccagatat tttgtcagca gttcatcgtt ggccaccaca tccgaatcgt agaagtcctt
     6301 cagctcatca ttttcgatct cataggtgcg cttccgcgac agggtgatgt cgtattttcc
     6361 ctcccccttt acggaataag agacatcccc cagagcggca tcgtaatata cgctggcaat
     6421 gggtgctctc cagtcgatga cctccggttc tgccgggtta tccgcaatga cacttctgcc
     6481 gatatagtag acctcgtctt ttttcagttt tttgtcccgg aaatcgattc tgccaaagta
     6541 gggctttttc ctcgcctgtt ccgcccggcg cagatcctga ttcacgaact tgaagcgggc
     6601 atccgcattg tgccacaggg tctgggcttc cttgtcgttc tcgtcgaatt cctcctgcat
     6661 ggcatgcagt tcttccgcca gagcctgtac actctgcctg ttggcatcca ccttgcgctg
     6721 tgcgatctct aacgtatgct gtaatctttt ttcctcatcc tgcctcgtaa tgccgggtaa
     6781 actgctcata ttctctcctg ttcaaaacca taaaaaagaa aaagcaaagg aagcctgtcg
     6841 aagacttcct ttgtatgtat tctaaatcac attttaccag tcagagcccc agtcatcgta
     6901 tcctgagtcc caactgtcat tggaatccca tccggaatcc cagctgtcat catcccagct
     6961 atcgttatta tcatcgctgt actcataagg agtgtaatag gacgaatcct caaaatcctc
     7021 atattccgca tcgctgtcat agctgtagga atcatagtaa ctctcgtaat cgctttccat
     7081 ccaggattgc gacggtgcgt attgccagga attatcctcc cactcgtacc agtccccgga
     7141 ataatagtaa tagctctggt ctccataagt gtagtagccg gtactcggtc cgctttcccc
     7201 tatgagtgca aaaataacgg acaatggtat gattgctatt attgctatca ggaaaatcga
     7261 aaaaagggtt ttggacaagg ctttttccga ttgcttttgc tgcgcagaag cagtaggccc
     7321 ggaaaccggc tttgcagctt tcggctgctg gtgcgccttc tgtacggcca ccctttcctt
     7381 catcttctgg taaagttcgt tgaccgcata ggcttcatcg gttcccttgt aacgtactgc
     7441 ccgggtaccg tcctctttat tcttatagac tacgaaggtg ccatcggtct catccttgta
     7501 aataccgaag gctttcgcgc ccttgtaatc ctctccgata aaggtccgca tgtcctccag
     7561 cggaaggttc atctcctttg cccaagcttt cagttcctcg attgtctgtg gtacaccgtt
     7621 ggcatggcgg ttgtaattgt tgttgacacc gccacagttc gggcagacct gatcaaaatc
     7681 gttgatccag ctgccacacc agtcgcattt tatcttcatg ttcccctcct gaaaatacac
     7741 gttgcaatag tttctgtttt attgtaccac gttctgcacc tgctgccaac ctaggaaatt
     7801 gccaaaagaa tgcggctctc cctgagggtc ttgctctggt cgtaatcata ctctatggtc
     7861 tggatactga catccggaaa acgggcatgc agccgctgca aaattccccg ctcgcagaca
     7921 tggctgacca gacatccgaa gggatgcagg atcagcacac tgccacagcc tttttccaca
     7981 tattgtatca cctctgcggc aatcagccag ccgtctccgg tattgcagcc ctccgccaga
     8041 atcccctgca ctttctcctt cagttccggg aacggcagat ccgtctggaa ccggtctgtc
     8101 tccccgatca ccgtaagcag atgtttttgt gcctcacaca accggtggag cacaaaggca
     8161 caggctttcg ccatgatggg attgcgctgg tacttttctg ccttataccg ctctgtatcc
     8221 accaaataca gacagtagtt gacgaaacca ccactgtaag gctccattcc ctgccggagc
     8281 agaaaacttt caatatcgtc atttcccagt ttcgaatatt tcatatagat ctcaccggtg
     8341 atgccaactc tctttttctt cccgggtttc accggtacct tcgaaaatac ttcgcaggca
     8401 taccggtaat tcttctctct ggtgtgccgt ccgttcctgt gtctccgaat gctctcgcac
     8461 agtttctttt ccacctgact gcggatgcgg tccgtctctc ccggattctg ttcgtagggc
     8521 ttcacctgct ggtacaggca catgatcaga tcgccatagc atccggcagc gatcccaccg
     8581 gacaacagcc ccggagtaat acgaaagccg gtatgttttt cctctcccat gagatttaag
     8641 gaaatcacgg ggatctgcgc atactccgtc tgcccctgct ccgccatgcg gtacaacact
     8701 ctctggagca gattgtagat attcccggct ctgcaggcac cacccgcctg cggctccata
     8761 aaagcgatct cccccggatc ccgctccccg ctttccagat attccaggaa ctgtgcaagg
     8821 atcaataccg tcggatagca atagtcgtta ctgatatagc gaagtgccag agtgcggtcc
     8881 ttcaccgggt tctttaaagt ctcgaaggaa tacccctccg cccggaatgc cgcctccaaa
     8941 aatacattcg tgtactccag catctcgcac agcaggatcg tacgggtctt cttgtcatga
     9001 cgtgtgaaat ttttacatac tctgtgcagt tcctccatac gttttcctca tctgaccggg
     9061 ttggtttgtg tccgaacata aatttactat tttatattgt aagctgaatc gttggcccgt
     9121 gcaagtatac atctggttta ttctggttgt ctaccatcgt ctgtaaactt tcaggcacac
     9181 aaaaaaagtg gaaaccttgt attttcaagg tttccacgct tttcaaaaga gctgctgacg
     9241 ggaatcggac ccgtgacctc cgcactacca atgcgacgct ctaccgactg agccacagca
     9301 gcatcacgtg actgctaacg caatcaccgt gttacatttt atcaaaggta tctcactttg
     9361 tcaatagaat tattttaaat tatatatttt ttttgcacaa ttcattcatt atgctttcgg
     9421 tgaccagtct gcgcatctct tctgggggca tatcatcacc gatatggtgg cactggtaca
     9481 tcagatagga aatcatccga gccaccagcg cgttggtaat ataagacagc ggaatgtcct
     9541 cccgcagttc tcctttcgcc cgtgcactgt ctatgatctg gcggaccaaa agatccaccg
     9601 gcccgttttc cttctgttcc gctatccgtt gccgcagaag atttccctcc ttggaatgat
     9661 ctgtcagcag atgcagaaac gccatgatgg catccacaca ctgggaatgt tcttccacca
     9721 gcaaaagcat tctgtggatc tgggtctcca gatccggaca ggtctgaatc tgacgggaag
     9781 cctgctccgt tactgtacgg atttcataca acagggcaca gacaatgatc tcttccttgg
     9841 aatcaaaata ttcataagca gttcccttgc caatgcccgc cagcccggtg atcatggaca
     9901 cgctcatttc cgagatatcc ttgtcctccc gcaaaagtgt gctgactgcc cggtacatgg
     9961 cggtcacctt gggaggcacc tggttcggtg cctccaatcc ggttatttta ctcattttcc
    10021 gcatcccccg tttttccaga tctttgagat tttgccagct tcttagccgc tcttctctcc
    10081 tgtctgctcc gcttatcgaa gatcagatag aaggtcggga tcaggatcag ggtcaggatc
    10141 gtggaagccg tcagaccgcc gatgataacc agtgccattc cctgcatcat gacaccgtta
    10201 tcgcccagtc ccagtcccat ggggatcatg gacaggatcg ttgtcaatgt cgtcatcagg
    10261 ataggacgca gacgggtact tccggaagcc accagcgcat cctcagtgga cattccctct
    10321 tcccgcagca tatttgcgga atctacgaac agaataccgt tatttaccac aatacccatc
    10381 aacatcagaa atcccatcag ggataccata ctaatggtac tttgcgtaat gaacaacagc
    10441 aggaaggatc cgatcagtgc aaaaggaata cacatcatga ccatgaagga gaatctgggg
    10501 gactcaaact gcattgccat caccaggaat accaggaatg tagctaccag aattgcctgc
    10561 aacaatgcct ggaactcatc gttcatcatt ccagtcatca tactgtctgc cgggattaca
    10621 gaatccggga ataccgtgtg atccaccaga tcgttgatag cattctgggc atcaaatatc
    10681 ttctcggagg tcatagtgga cgtcacgctg acctgataca gaccgtcctg cttcatgatg
    10741 gtctcctgac tctgtgcgta attgatctct gcaatatctc ttaacggtac cacggtgcca
    10801 aaagaagtat ccagtcccag atccatcagc aggttcagat catcatagct tccttccgga
    10861 tattccagcc ataccgcata ctccgaacca tcattggtga tcgtcaacgg attcataccg
    10921 cttaatacgt tatgtatggt catgccgacc tgaatgggtg tcagcccgta ctgcattgcc
    10981 ttcagaggat ctaccactac cttggccagc gtcgtagagt ttgccagaga gttttctgtc
    11041 ttaacgacac catcgatctt ctcgatttct gcctgcagat cttctgccgc ctgcgccagt
    11101 tctgccctgt cactgccctg taaatcgatc tcatagctgc tggtactcat catggtgctc
    11161 atactgctgc cggaggatgc caccgtaaca tccatatccg tcattccggc tgcgatctca
    11221 ttccatttat ctactacctg tgctgtggtc agggttctct tcttgatcag atctgcggta
    11281 agggtcgcac tggagccgct gatggatacg gaataagatt ccacatccgg ctcttcctca
    11341 gcgatctgtt cccacaattt cagcgcttca tcctcttttt ccagagtagt accggaacgg
    11401 aagttcaccg taaccgccac gacgccttca tccgctgcag gaatcagctc catgtccagc
    11461 gttcctgcca gcagaaccgt gccgatcatc agagccacag ccacaccaac gacggtcctt
    11521 ctgcgcttca tcagcttccg caggatcttc tgataccatg tggtaaattt cttcaacagc
    11581 ttatcgatgg gcagattttc tttttccttc ggtttaaata ctgtgtagaa caacggtacc
    11641 agcatcatag ctgcgatcag ggatgccagc atggcaaata caatggtgaa tcccagctgc
    11701 tcaaacatct gtccggacag gcccttcata gtggccagcg gcagatatac cacgatggtg
    11761 gtaatggtgg aagcgataat agaagccgtc acttccttcg ttccctctaa ggctgcctgt
    11821 ttcagatccg gcgtactctt ctgtcttctg aaactactct cgatcacgac gatggaagag
    11881 tctaccatca taccgatggc aatgaccaga gagcccagtg ttacgatgtt catggagaat
    11941 cccatcatac tcataaggat cagagtcagg aacagggaaa taggcatgga ggctccgacg
    12001 atcaggctgg ctttgaaatc accgaagaac aggaacagca ccgccatggt caatacgaca
    12061 cccaataaca gggtctctgc cacactggtc agagcgctga taatggagga gctggcatca
    12121 taggtgacct caaattcaat ggccggattc tccgcctgga tctgctgtaa tttttccttc
    12181 acatcccggc aggcatttac cgtaccggca ctactcttgt tcttaatgcc gatgctgaca
    12241 ttgtcctgtc cgttgtaacg gctgatactg gaagcatcct tggatgctgt ggtaacattt
    12301 gcaatatcct gtaaggtaat gacctgtcct ttggtggtca tcaggggtac ttcccgcagc
    12361 ttctgtaccg tattgtactc catggacgtc gccacgctga ggtcctgact gccctgggtc
    12421 acgctgcctg caggataagt aaagtctacg gttcccagca tctgtgccac actggacatg
    12481 gtcagaccgt actgcttcat cagggtctca ttgagttcta ctttgatata atcctcggaa
    12541 ccaccggtga cggatacctg tgccacgctg gacagagatt ccagttccgt taccacggat
    12601 tcattgacta cttttaacag atccacatcg ccgacttcca ctgcggagat cgtcatgaca
    12661 tccatactgt tcatgttcat ctcaatgatg gtaggctgat ccacgtcatc cggcagactt
    12721 aacttcgccg tctccatggc cgcacggaga tcgttgtgac attcatcaat atccacaccg
    12781 tattcatagg taaacaacac catgcaataa ttttcaaagg aataagaatt cacggaatcc
    12841 acaccgctca gagtggaacc ggaatcttcg ataacggatg ccacgagttc atccacgctc
    12901 tccgggtctg cgccgggata ggtagccatg acaatgagca tgggcatctc aatgtcgggc
    12961 gtcagttcca tcctgaaacc aaaaatggag ctgatgccga atactgccag tgccaggatc
    13021 acaaggaaac aggacaccgg tcttttcaga gctagctttg tcaaattcat tctgctgcct
    13081 cccctactct tctgccttca ccggtgcggt gacttccaca ccgttcatca gcttgggaga
    13141 ccagctggtg atcacggtat ctccgggctg tattccctca gtaacagaaa tctgtgtatc
    13201 atcaaacaga tctgtggtca cataggtctt cacggccttg ccgtccacac ttaagtatac
    13261 ataagaaccg gtattatcat agtacacggc atcataaggg atcagcaccg cactgtcgtt
    13321 cgtatgatag gtatcggtcg tcagcttcac ggatacaccg ctgggcagat cgctgccatt
    13381 ggcatttacc gctgccttga tctggaacag gccggtctgc tgattcactg ccacaccgat
    13441 ttcggtgatg ctgccagtgt agctggtggt tccccgctcc acggtcactt cctgccccat
    13501 ggtcagtgta tttttcacag cttcgctgac ctgaaaagtc acggtcatca tattttcatt
    13561 ggcaatggtg tacgcaggat tgctgcttcc ggcaatgccg ttgacttcca ctcccttgct
    13621 ctggattgta ccggagatgg gagccttgac agtgtagtag gataatgcca gttccgcact
    13681 ttccacgccc acatttgcca gctgttcact ggtggacagc tgcttcttcg tatcctccag
    13741 tgcctgtccc tgggacaatg cctgggaacg atccacaata ttcatgctgc tctttgctgc
    13801 attgtaagcg gattccgcct gctgtaatcc ggccaccaga gaatttctgc tggactccag
    13861 ctgtttcttc gttcctttca gtgtctcgat ctgcgtcttc atctgcacaa cacttgacgc
    13921 atttccgccg ctcacactgg atgacgctgc ttttatggca ttctccagct gtgtaatatt
    13981 tgcattgcat gcgtcgatct gatctgaaag atcatcatag ttctggtcat aggcattttt
    14041 cgcctggaca tacgcgatct gtgcctgatc atacccggac tgtgcctgca ctctgctgct
    14101 atccatctgg atattggcgg attcctgctg ggtggtcagg gcactgtctg cctgctgcct
    14161 tacattggcc gcactgagct gtgcctgttc taactgcagt tttgctgcag agtcatcgat
    14221 tttgaacaaa acatccccgg cattgacctg atctcccacc tcaaaataag tatccgtcac
    14281 tgtaccggaa gcaaagggaa tgacatagac catctcctgg ggagaaaccg ttcccacaaa
    14341 gctgttctgc aaggtcagtt ttccggtctg ggcctgcttc atatccacga ctaccgcaga
    14401 agtctccaca gcatcctgtg ccccattgct gccgcagcct ccgagcaaca gactgcctcc
    14461 catcaacaac gccattccga cgctgcaatt tcttttccat gtattgtgca tgatcttttc
    14521 gcctttctga atagttatga atatgtattt gctttattaa ccgaccggcc ggtcggtcag
    14581 catttgcagt atactcctat gctgggtttt aagtcaatat tccaactctg cagattctat
    14641 taaaaaacta taaaaaacac attcctgaaa aattttttca agaatgtgtt ctcttctctc
    14701 attattttgc atcacaccag tctccggatt ttgctcttca gacgctgtaa ggcattgtcc
    14761 gtagactttt cgtctcttcc cagcaccttg gcgatctggg tatagctcat tcccgtcagg
    14821 tacaaatcca gcacctgttt ttcaaagctg ctgagttctg tctcaatagc tctctccaga
    14881 tcttccaccc gctccttgtc caggaacagt tcctccggac tcatctgggt tctggccgcc
    14941 agagcctcaa taagattttt ctcctcattg tccgtgccct cgctgcttcg ctcatacaga
    15001 gatacatagc tgttgagcgg tgaatgcttc aggcgcttgg aagcctgcac tgccgtgtac
    15061 atctgtctgc tgatacacag atctgcaaag gtataaaagc tggcatctct gccgcagtca
    15121 taatcccgca ctgccttgaa cagcccgatc atcccttcct ggatcagatc ttcgctgtct
    15181 ccgcccagaa tatacatgga ttttgccttg cttcgcacca gatttttata cttgtcgcag
    15241 atgtaatcca tcacctgtct gtcaccttcc cgcagctggt cgatgagttc ttcatccgtc
    15301 atctcttcat atttgaaatg ctctgccatg ctttcccctt ttttccactc ctatattacg
    15361 cctgtaatct ctgacgcacg atctcataag ccagtacgcc tgttgccacg gaagcattga
    15421 gggaatcgat atcccccttc atgggaatgg atgccaccat atcgcatttt tccttcacca
    15481 gacgtcctac gccctcacct tcattgccga tgaccagacc aatggggcct ttgagattca
    15541 gatcatacat ggtggtaccg cccatatcgg cgcacacgaa ccacagtcct tcctttttca
    15601 gttcttcaat ggtcttggcc agattcgtca cctttgctac cggcgtatag ttcagcgctc
    15661 ccgcggaggt tctggctacc gtcgcggtca gtcctgctgc gcggttcttc ggaatgatga
    15721 caccgtgggc tccggccaga ttggcggtac ggatgatggc tcccagattg tggggatcct
    15781 caatgttatc caacaggaaa ataaaaggcg gttccccttt ttttcttgcg ccttccagaa
    15841 tatcatccac ctccgcgtat tcataagctg ccgcataggc aatgactccc tgatgctttc
    15901 cggtctcgga taactggtcc agacgctcct tggtcacata ttttactaat gtatcatgct
    15961 tttttgcttc tcttttaatg gtcatcatgg ggccatcctg acagccatcc agaatgaata
    16021 acttgtcaat gggtttgccc gcacgatagg cctcgatcac ggcatttctg ccttctatgg
    16081 taaattcctg atatcccatg tttgtccttt ctattgtgaa tttttattac agttccatgc
    16141 ctgccagacg gatgccgtct ttgatcaact gcagcatccg gtcgatctgt cccgtcaggt
    16201 acagatagcc catcagtgct tcaaaaccgg tagctctgcg gtaatctgcc acggaagcgt
    16261 ttttcgcggt ggtgtatgat ttggcgttgc gtcctctgcg gtacaccgct tcctcttcct
    16321 ccgtcagttc cagaagcagt gcatcgatca tggcagactg cgccggtgcc tttacataac
    16381 gtgtcgtctt tttatgcaga tcattggcgg aacggttagc tctctccacc accacgctgc
    16441 ggatcaccag ctcataaatc ccgtctccga tataggccag tgtcagcgga gaatacgccc
    16501 ggacatcctg tccttcgcag gcaaatgctt cctttatttt cgtaagaagg gttatgctct
    16561 cttccatttt acaccctctc tggtatcttc cagcacaatg cctttggcta acagctcatc
    16621 acggatctcg tcggctcttg cgaaattctt cgcctttctg gctgcctgac gctcttcaat
    16681 cagtgcctcg atatcgctgt ccaacatttc cgctttcttg tctacgatca gtccacagat
    16741 atcggacaga gtcacgattt cctgtttcag agcccccaga aatgccttgc tgctatctgc
    16801 tttcgcattg gagttgacga attttaccag ctcgaagata gctgccagcg catccgcagt
    16861 gttgaaatca tcgtccatag cttcatcgaa tttggtctca aagccctgtg cctcggtaac
    16921 taactgcttc tcttcttcgg acatttcggc atctgctgcc ttttccagca ggaacttcag
    16981 gttatccacg ctggtaacga ttctctcgta gccgttcttt gctgcttcca tcagatcggc
    17041 actgaaattc agcgggcttc tgtaatgtgc ggacagcatg aagaaacgaa gtacctgcag
    17101 atcatatttt tcggagatct cacgaacagt aaagaagttg cccagagact tggacatctt
    17161 gcgattgtca atgttcagga aggcattgtg catccagtat cttgcaaaag gcttgtcatt
    17221 ggctgcctcg gactgtgcga tctcattctc gtgatgaggg aagatcagat cttcgccacc
    17281 ggcatgaata tctatctcct cacccagata acgcttactc ataacagagc actcgatgtg
    17341 ccagccggga cggccctgac accagcagga atcccagtaa ggctcgcctt ctttcttcgg
    17401 cttccacaat acgaaatcca ggggatcttc cttctgatct tcaccggaga ctaacaggga
    17461 acggttaccg ccctgcagat cgtccaggtt cttgtgagac agcttgccgt agtccttgaa
    17521 gcttctggtc cggaaatata ccgtaccatc cgctgcggga tacgcatgcc ccttgtcgat
    17581 cagagtccgg atcatatcca gcatgccgcc gatctcctcg gtagccttag ggttcttggt
    17641 agcgggcttg atattcattc cctccatgtc ttttttgcac tctgcgatat agcgctcgga
    17701 gatcacgctg ggctccacac cctcttcatt ggccttcttg atgatcttgt catccacgtc
    17761 tgtgaagttg gatacatagt ttacttcgta acccttgtat tccatgtaac ggcgcaccgt
    17821 atcaaatacg atcatggggc gggcattgcc gatgtggatc aggttgtata cggtaggccc
    17881 gcagacatac atttttacct ttcccggctc caggggctta aattcttctt ttctcttgct
    17941 taaggtgttg tagatttgaa tactcatgat ttatcctttc ctgtcttaac tgtattatca
    18001 gttttcgctt cttcgctcat taccaccagc ctgcgcagct gctttaattc atcctccatt
    18061 tccagtaccc ggttggtcag ttcggaattt tcacgctgca ataccgtgat gtcctcccgc
    18121 accggatcgg ggaggtctgt ctggttcatg gtctcctgag ggagacttgc gttgttacgc
    18181 tttactacgc gtcccggtac acccacgacc gtggatccgg ggggcacttc acttaagacc
    18241 acgcttccgg caccgatctt cgaattatct ccgatggtaa aggaacccag tactttcgca
    18301 cccgcactga tcatgacgtt gttgccgatg gtcggatgac gcttgccgtg ctccttgccg
    18361 gttcctccca gtgtcactcc ctgatacagg gtcacattat cgccgataat ggtagtctca
    18421 ccaatgatga caccgttgcc gtgatcaatg aaaaatccct tgccgatctg cgcgccggga
    18481 tggatctcaa tgcccgtctt acgtacacca cgctgggaaa tatatctggc ccagaaataa
    18541 tgcccgctca catatagctt gtgagccagt cggtaatgga tcatgacctt gaaactggga
    18601 tacaaaagta cctccatgga agaatggatc gccgggtcac gctccctgat aatacggact
    18661 tcttctttta aatttttgat gatgcccatg ttctcacctc gaaatatact tcgactattc
    18721 agacatcttt tgcaaattga tatttgaatc gtctcaaaaa aataccgcag acaaactctt
    18781 ctcttagaga cgaatttatc cgcggttcca ctctacttaa gggacgatgc ccttcactct
    18841 ggcgtaaaac gccatgcaca taacgggtgc ttaccggatc cggatactga ttactgttaa
    18901 cgttccccag atcagctcac aggcgcactt ccttttcctc agacgaaagc cgctcccagc
    18961 ttctaacagc ttctctctgg cgtcccaccg aaaagtactt ttcctgctcc atgcctttac
    19021 tgaatatacg atcattccta ctaaataaga tatgcaaaaa tacgggtttt gtcaaccccg
    19081 gagttttcta catcactgct ttttccaacg tttccctcac atattcattc agtgacatct
    19141 ggcatgcagt tgcatggatc acaagctgct tatgagtctc aggtgcgatc cggatattaa
    19201 agcttccctt gtatgctttt tccggagctt tcccctgtcc ttcacacagt tgcagataat
    19261 catctaccgc cccatgaaaa tcctctacca gactcctggc atcggttccc tcataagaga
    19321 tcagggaacg aatccccatt acctttccga aaaaaatccc atcctcctcg gaaaattcta
    19381 cgctgccgac atacccttta tattccatcg tatttttcaa atcaatccct cctgttccaa
    19441 cagatcacat aattgtctga cctggtatgc tttcaattcc ttttgcggat gcggcttatg
    19501 tagtaatata ctcccatgct cctcactggt aaacatcact ctggaaccgc tggttttccc
    19561 cttattggat ttcgtaaatt ccagataatc caataaagct tccgcatcat gaaaggtaaa
    19621 tgtcttcggt ctgcttttta actgttcaat tagtttttcc ttttgtccca tttgtacccc
    19681 ttgttaatta tattatattt tttctgcttg ttttttgcaa ctgtttttag ttgcaagaat
    19741 gaagttaaaa gcatattttc tacctaattt caatggattg gaaaatttct ccgacattgg
    19801 attctaatag aaaacagatc tgataataga aaaaggccct atgccattta tattagcata
    19861 gaacctttgt aatcacaata gtattttatt cttccaaaag cacccactgc ccctccggtg
    19921 ccgcagctgt tttctccgtc agctcttata atcctcgatc atagcttcga tctcggcgat
    19981 ctgggccgcg ctgagcttct tgctctggat aaatgcggcg atcatcttcg gcagcgaacc
    20041 gccgtaagcc ttctccacga aagcctcccc ctgggctctg tggtattcct ccttattcaa
    20101 tacggaagat acgatggcat tctcattttt gaaaataccg ttctcacaca gctttttaat
    20161 gaacgtatag gtagtggatt tcttccagcc gaacttctcc gcgcactgtt ttaccagttc
    20221 tccggaaccg atgggttcct cctcccagat cagctctgca aatttgtatt ccgtctccgt
    20281 catctgatac atttccattc ctccttgtat ccttcttaat ccagcccttg ccaattatgc
    20341 aatcctattg gcttttctgc actcatcaca ttataaaagg tcgatagtat acagaccttg
    20401 ttctaatcaa agtctatatc ctatcgacct atttgtcaag ttcttatttt ccgaaattat
    20461 cagctcttcg ggcatccgct gcagcttcca catcccccct gcaccgcctg cgcagtgagg
    20521 tcaaagggcg tagtcaccag acagattgcc tgagaggaga ttccctctcc gctgccggta
    20581 aatcccagtc cttcctcggt agtggctttc acattgacct gccccacatc gatcagcaga
    20641 gccttggcaa tattttcccg catggtatca atgtgggggc gcatcttcgg tgcctgcgcg
    20701 atgatcgtcg catcaatatt ttcgataaac acatgatttt cttccagcag ctcgcccact
    20761 ttttccagca attttatgga agaaataccc ttgtatgcgg gatccgtatc cggaaaatgc
    20821 tttccgatat ctcccagcgc cgctgccccc agcagcgcat ccattatcgc atggagcagg
    20881 acatccgcat ccgaatgtcc cagaagtcct ttttcataag gtattttcac accgccaatg
    20941 atcaggtctc ttccctccac cagacgatgt acatcatatc ccattccgat tctcatactt
    21001 acaacctttc cgcagacgtt atcctttttc aaaaagtact gacatccctg tgtcttaccc
    21061 gattctccaa ccagccagtc cccaaaccgt tctgtcccgt tgcggccagt gccgggtgcc
    21121 aacaatgtcg tctgcaatat acgaaatctc tgtcattatt ttctcgccac agcacagtcc
    21181 atcagatcct ccgtactgtc aaatacatag accgtataac tgccgtcctc atacacctta
    21241 ttgccctgtt tcagcgcagg cgccgatgca tattccgcgc attcctccga cgtcagaagc
    21301 aggaatgtct ccccggtatg atacccttcc tcataatatt tcatgggaga agaccacttg
    21361 aaatattcca gatgttcggg atctccgatg ttgccgatct ccacttcgcc attggtaagc
    21421 tctgtaatga tattggcatt attataagta gcaaagccaa aatcataccc gttttccgca
    21481 aggaactgcg ccacgggacg cttcgtttcg ttcttgtcca ccgtcaggaa agacatcacc
    21541 gttttgcccg ttccaaggat cagacagatt gtaagcaacg ccgtcaccgc cagacggtca
    21601 aatggcattt tctcctcttc cagataaaag cacaataccg gcagtgcaaa aataaaaatc
    21661 gtaatataat accgcggcac catggtgctg gtggtgaaaa taaacacaaa cagatttagg
    21721 acaaagctga ctttcaggaa aagtgtgatc aggctgcgaa atcctgtact ctgctttatc
    21781 tttccgttct tcaccgtaac atatccgtag atgcccagca ttacgaaggc cgccatggtc
    21841 acaataccac gcagggataa aaagcccttg tcgggaatat aaccaaaaag cattaacaga
    21901 cagcctactg cattctggat ccggtcaaac agcaccccgt ggtacagagc aatgaaattc
    21961 gtggcaccgt aggtctggaa cacatattta tggctgatcc ataccacatt aatgccgtag
    22021 cccgctactg tgaaaaacgc tcccactaca ctgtagagaa aatatctcat gcgggtcgta
    22081 ggcagcaagg cacgaaaacg ctcctttgtc atctctcccc gaaaaccctg gaacttctct
    22141 ccactcaaca gatagaaaaa ggaagtcagt accagcgggc actgtaatgc cagcaggtaa
    22201 cgtacgccgg acattccaca gacaatagcc agtagtacat agaagatcca caggctgacc
    22261 ttgcgttttg tactgtattc tccactgcac agccgcagat acataccgaa gaaccacaac
    22321 accagaatca catgggacat gtaggtattg cccatttgca tgtgtgtcat catggtctcg
    22381 gaaaaaggca gaagcaacag acaggagctt aacaccgtaa gccctctgga tacctttaac
    22441 ggcttcataa aataataata ggaagccagc atcagcccgt aaaacaccag attcgtgatc
    22501 gtgcggatca catgccagtt gttgcagatc cggaacagcg gtcccatgat cagctgtgta
    22561 tacagcacac ggaactccgt ggaataatac cagttcgtcg ttgaaatcat ctgatgctcc
    22621 cccgcaagga tccgggaaaa gatcatctct gccgccatat cggaatccag ccagtgatcc
    22681 tggctgataa tattaagtcc caaaaggagc agaagtgtgg ctcccaacag gatataaggt
    22741 atctgttttt tcagtgtttt cataccgcag acctttctga attatgccta tctattcttt
    22801 cctaactata cacgatttgc cgcgaaagca caactgcggc ttttgcggca aatacataaa
    22861 attcctcatc tacttaagat ttttttctct tggggaagct gttaccgatg cccggctctt
    22921 cccgtttctt tctctttttc gcttctcttt ccgctctgtc cccggagtat ttgcggtcag
    22981 ctgtctttct gtcgcggccg ccccattttc ttccgtcctc gcggttctcg tctctgcgac
    23041 gtcttctgtc ctctccggaa ccggtgctgc catcatctct gcggtctctg tcactacggc
    23101 cgaatcttct gcggtccccg tcttcgcggc tgaacctccg tcttccgtct cctctgccga
    23161 aaccacggcc ttcgccatcg tttctgccga aacgtccctt ctctccgaac cgtccgcggc
    23221 gctgtacata atcatctgcg gcgatctcct cgcccttgtc gccgacctga tatttcagca
    23281 tggctgccgc cagatccagt gcatcgcagc cttcctcttc cattttccgc tgtaaaaagc
    23341 tcttcatcag ttcaacgtct tcgtgctcat gcagttccca cgccttgttc agatatttat
    23401 ccgctttcgc tttcagaacc tttgctgcgc cgggaagctt tttctcggta atggtggtat
    23461 gacagactct ctcgatctcg cggatcttac cgatctccct actgttggca aaggtaaagg
    23521 atctgccctt tcttccggca cggccggttc tgccgatacg gtgcacatag tactcgatat
    23581 cctgcggaat atcgtaatta atgacagctt ccacatcatc cacatcaata cctctggctg
    23641 ccacatccgt agcgatcaga atgctggctc cgccgttacg gaaacggttc atggccacat
    23701 cacgctgcgc ctgggacaga tcgccatgca gcccttctgc ctggaagccc tgctgcttca
    23761 acagatccgc cagttcgtcc acctttttct tcgtattaca gaagatcagg gtcagcttcg
    23821 gctgatagta ctctaacagc cttacacagg ctgcatcctt gtcctgattt ttcacacggt
    23881 agaatttctg ggataccaac ggaatcgtca gttcttttgc cgctactttt actatttttg
    23941 catccttctg gaaccggtcc gtgatctcca ggatcggctt cggcatggta gcggaaaaca
    24001 gtgccgtctg gtgctctccg ggaatctgtc ccaagatcag ctccatatcc tcacggaagc
    24061 ccatgttcag catctcatct gcctcatcca ataccaccat gttcaccaga tccagtttga
    24121 tggtatgtct gcgcatgtga tccatgacgc gtcccggggt tcccacgata atctgcacgc
    24181 cccgcaatcc tgcgatctga cgactgatat cctgtccacc gtaaacggga agcaccttga
    24241 tgccatgcat gtatttagcg attttccgca gttcttccgc ggcctggatt gccagttctc
    24301 tggtaggaca taaaataatg gtctgtaact tcttcacatc gggattgatg tgctggatcg
    24361 ccggaatgcc gaatgcagca gtcttgcccg ttccgg
//
LOCUS       DAWDHD010000022        23677 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_162_length_23677_cov_14.771696, whole genome shotgun sequence.
ACCESSION   DAWDHD010000022 DAWDHD010000000
VERSION     DAWDHD010000022.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 23677)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 23677)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..23677
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_162_length_23677_cov_14.771696"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         6566 a         5147 c         6072 g         5892 t
ORIGIN      
        1 tgccgcgttt cttataccct gccaagggct acgggattat ttcactaact ctcaagttaa
       61 tataagacgg gtgagcttta cagctcaccc gtcttatatt ttgcaacgat attctgaatt
      121 ctctcatttg gattcaacag atcgctgatg gaagaatcgt ggttcaaagt atcaatgatg
      181 taagcaatgt acttactcat atcacagttg atataccact ctctctgaag cagttccgga
      241 gtctggtaga tcaggttggt ggtcagaacc ttgtcgataa tgccgttctc gtaagccttg
      301 tcgaacttgt ccagaccgtt ggtgaagagg ccgaaggtgg agaatacgaa gatcttgttg
      361 gctttacgct ccttcagagc agcggcaact tccagcatgc tttcaccgga ggagatcata
      421 tcatcgataa tgatcatatc cttgccctct acgctggtac ccaggaattc atgagctacg
      481 atggggttac gaccgttgac gatgcgggtg taatctcttc tcttgtagaa catacccata
      541 tccagaccga gaacgttggc aatgtagatg gcacgtccca taccaccttc atccgggctg
      601 atgaccatca tgtggctgga atccagctgc agatctttta cattgcgtaa cagacctttg
      661 ataaactgat atgcaggctg tacggtctca aagccgtgca gaggaatggc attctgtaca
      721 cggggatcat gagcatcgaa agtgataatg ttgtctacac ccatgcttac cagctcctgc
      781 agtgccagtg cacagtccag agactcgcgg gaagttctct tgtgctgacg gctctcatac
      841 aggaagggca tgatcacagt gatacgacgg gccttaccgc cgacagctgc gatgatacgc
      901 ttcagatcct gatagtgatc atcgggagac atatggttct cgttgccaca cagggaatag
      961 gtcatggagt agttcaggac atctactaac agatagagat cagtaccgcg gacggattcc
     1021 agaatctgtc cctttgcctc accggaaccg aaacggggta ctttggcatc cagaagatag
     1081 gattctctct gataacctgc aaatgccaga gactctttgt gttcgctttc tctctctgct
     1141 ctccatttta caagatactg gtctaccttc tcacctaaag gcttgcagcc gggcagggaa
     1201 ataatgccca gagagccgac ggggatagtc tccagatttc ttttttcttc acgattcgcc
     1261 attggggtga tcctcacttt ctgattctgt atactgttct gttacaaaca aatgattaat
     1321 tgtcagatgt attctgcata cgctttttgc acatacgaat atcggggccg gtcagcttac
     1381 acagatcgta atgactggtg atacgggaaa atacccggtc tgaataccgg tcgcgcagtt
     1441 cctccagact caaatttgtg gagatgatcg tagcgttttt ccgcagatgc ctctcattca
     1501 gacaggagaa cagctgggag gctacaaaag tattggtcat ctctgtgccg aggtcatcaa
     1561 tgatcagcag atcgcagtta tacagatcct cgtagaatcc ggagagagct tcttttgccc
     1621 tgctgtcgaa ggcataacgg gcaagagtat caaacagccc ggaagcactg aaatagatca
     1681 cggaatgacc ggtttgcaat aattcactgg cgatacatcc cgacaaaaag cttttgccag
     1741 tacctactgt accataaaag aataaattgt ggtagtcttg tttgaagttt tgtacaaaat
     1801 tgcgacagac atccacgcat tttcggaaat gcgtaagatc atcgccctga tagtattcgt
     1861 aagaaaggtt ggaaaaattc tcagaagcga tcatatcccg gatatgggac tgctcataga
     1921 gaatatccag ttcctgctgg tgaaaacagt gacatttttt gcgaagacca tcttcactgt
     1981 caatataacc tgtatctttg caatcggggc agtcatagac gggttccaga tagtctgctg
     2041 ggtatccggc ggacgtgagc aattttcttt tctgacggga gatttccttt agagaagaac
     2101 gcagtctggt aagcgcctcg tcatcaccgt tcaataaaag ctttgcctgc gccacggaaa
     2161 gttcaccgac agactcatcc agtcttccgt attccggaag ctttgtataa atttcccggc
     2221 ggcgctgctc tgccaggtga cggttgcggt ctctggtctg ctcatagccc ttcataatag
     2281 aattgtattg tgcgttagtc agtgacacgg tcggttcccc ttatctgctc attgttggtt
     2341 gttcaatatc tgaatattag ttacttaaaa tttccttttc cagagcggcg aaatcgtagc
     2401 tgttctgggt aaactgtgta aagcggttgg aggacccgga agaagcgggc ttcgcagtct
     2461 ttttcttctg gaaagagtca tccatctgct gaatatcggc cttgtggtgt acattagcct
     2521 gtctccagct gttcaggatt ccttccgcat attcaaagcg atgcttgtcc gtagccagag
     2581 agctgcgctc acaggcttcc agtataatat ctgtgctgaa accgtaatct ctggtccaac
     2641 gattgatgaa ttccagttct ttcgctgtgg gagaggtact gcgtcctaag ctgttcataa
     2701 tggaatatac gcttctgtcg tagcgggtgg agaatttctg cgcctgcttc ggggtggtga
     2761 ttccttcttc cgcccagttg acagcaactt tttctatgta tttgaaatct ttcttgccgc
     2821 gctccacaca gtactgtagc agataatcga tcagatcctc tgaaaaatgc agtacatcgg
     2881 tgaaaaacag gatcgtcttg atctcggaag gagtcagagg cttgccaata taagcttctg
     2941 caataaagag aagctgggag gtctcttccc gttccttgaa ttcccgtaac tgatccagag
     3001 aataagccgg ctttgcgtaa gggttgacag tttcctgggg tgttgtcggt acaactgcag
     3061 ccgaaacctg cggaatagca gcggcaggaa tcacagagac tccggcggga gtattctgca
     3121 tctgcgaagg accggaggtc agcaggacat tatgctgggg ggcaggagca gcctgcgtgc
     3181 tcagatcacg aagatgaatc cctaccagtg ttttattatc atcgaagtcc agatccagga
     3241 tctgctgctt ttcccagtat tttagtgcac ggatcacatc cttttccgtg tggttgaact
     3301 tgtccgcaat tccggacaca ctgatagcct gttccgcatt cagcatgcgc agcagataaa
     3361 aatagacctt cagctgtgcg tcgttggcat ctttcatata ttcatcaata aacagattgg
     3421 atactaccgt agaatctgca taattatctt tatagagcgc tatccgtgcc ataacacaca
     3481 ataccttctt tcacaaatcg ttcctttttc aaagtaagac caagtatagc ataagacctg
     3541 tgaaaaagaa atagtcaatt tgccggaaaa ccttgtaaac actggacttt cggactcctg
     3601 gataaaaatg tggaaacggt ggatattgtg gataccaaaa gacggtgtat cgagaaaacc
     3661 ctatatatcg gcagatcatc gccaaaatcc gacagaaaat tatccacagg aaaacaacaa
     3721 aatatccaca gggaaaacgg ggataatccc cggtgtttcc tgtggataat gtggacaact
     3781 atttgccaag caggttttca ccgattttta ctacatctcc ggcacccata gttatcaaca
     3841 ggtcaccgtt tatgcaattt tctaataaaa agttttcaat ctcatcaaaa gaaggaaaat
     3901 agtaacattc atggcccagt gcccggatct ctgtctggag cgtctcagaa ctgattccca
     3961 gggtatcggt ctcacgggca gcataaatat ctgccaggac gactttgtct gccagactta
     4021 aggctttggc aaaatctttc atgaatgcct tggttctggt gtaagtatgt ggctggaaga
     4081 cacaccagag ggtcttgtgg ggataattcg ctgccgcatg cagggtggct gcaatctcgg
     4141 tgggatgatg tgcataatca tccacaatgg tgacaccgcc gatggtgccc ttgtattcga
     4201 atctgcggtc tgtaccggtg aaaccgtgca gggcagaagc ggtaacctcg cgatcgatct
     4261 tcagcaaatc ggccagggca atggaagcaa tggcattgca tacgttatgc tctccgggaa
     4321 cctgcagaga aaaactgccg gacacttctc catgccggtg aagagtaaag gaaggatgac
     4381 cgaactcgtc aaaagtaata tccgagggat aataatcggc agcggacccg tcactgttga
     4441 ccgcttcttt attaaaagtg atcacgcgac agtccagatc tcctgtgatc tcctgtaatc
     4501 tgtcaatggc accgttgatg atcagacagc cgtcagcggg cagaagcttt gcaaatttat
     4561 ggaaggaatt ccggatatcc tccagatcct tgaaaaagtc caggtgatct tcttcgatat
     4621 tcaggatcat accgatcctg gggaaaaagc ttaagaagct gttggtgtat tcgcaggctt
     4681 ctgtaacgaa cagatcggta ctgccgacac ggatgttgcc accgatgctt tttaacattc
     4741 cgccgatgga cagagtggga tccgtaccgg catgcagcag aatctcactg accatggagg
     4801 tcgtggtggt cttgccgtga gtaccgctga tggcaatagg agtcttgtaa ttctgcatga
     4861 tctctcctaa aagctgcgct ctggtgagat gcgggatccc tttttccata gtagcgacat
     4921 attcgggatt gtctttgtga atcgcgctgg taaatacgac acaatcgata tcatcggtaa
     4981 tattcccggc acgttgtcca taaaatacat tgatcccctt tctttccaga gtttcggtga
     5041 gggggctttt ggaacggtcg gaaccggaca cacggaagcc ggcatccttc agaatctctg
     5101 cgagaccgct catgctgata ccgccgatac ctacaaaata aatggacgag gggtgattaa
     5161 agtctatctg atacatattg gcacttcctt aatatagtgt agttgcattg cttattatgt
     5221 aactattcag agcagttacc ttattattaa tattatatac ataggaacga aaaaaaacaa
     5281 tccataggta cgattttgaa aaaatgtgta agtttactat tttttgcaaa accaatccag
     5341 acgggattgg cataaattga caaaaagctt acaaaagata agctaattat gtaaaaaatg
     5401 gatatgaaaa tcgtaaaatg acaaaaaaac ctagtaataa atattcactt tcctttggca
     5461 atgtgttata atcagattat cacatccgaa ggaaagagca ggcacttttt cgagacagat
     5521 ggatacatcg gataatagcc gcgagcgact atttattaca acaggtggaa ggctagatag
     5581 aggtgaggat atgattaaga aagaaatgat agccatgcta ttggctggag gacagggaag
     5641 ccgtctgggt gtcttgacat ccaaggttgc taaacctgcc gttgcatttg gaggcaagta
     5701 ccgcattatc gattttcccc tttctaattg tattaattcg ggagtggata cggtaggcgt
     5761 actgacacag taccagcccc tcagactgaa tacccatatc ggaatcggta ttccctggga
     5821 tctggatcgt aatatcggag gagtgaccgt gctgcctccc tatgagaaga gtaccaacag
     5881 tgaatggtat accggtactg cgaatgcgat ctatcagaac ctggagtata tggagagcta
     5941 taatccagag tatgtgctga tcctttccgg agatcatatt tataagatgg attatgaagt
     6001 gatgttggat ttccacaagg ccaacaatgc agaagttact attgcggtaa tgcctgttcc
     6061 catggaggag gccagccgtt tcggcatcat gatcgcagat gagaaccatc gcatcaccga
     6121 atttgaagag aagccggaac atcccagaag caatcttgca agtatgggta tttacatatt
     6181 taactggaag acattgaagg aagcactgat cgctatggca gatcagccgg cactggattt
     6241 tggtaagcat gtcattcctt actgccatga aaagggggct ccgctgtatg cgtatgagtt
     6301 caccggttat tggaaggatg taggtacttt gagttcctat tgggaagcca acatggaact
     6361 gatcgatatc gtaccggaat tcaacctgta tgaggaatac tggaagatct ataccaagag
     6421 cgagatccag ccccctgatt atattgctgc cgacagtgtg gtagagcgca gtatcatcgg
     6481 tgaaggctcc gaggtctatg gagaagtata taattccgtc attggttgtg gtgttacgat
     6541 cggtaaagga accgtgatcc gggattccat tatcatgaac cagacccaga tcggtgaagg
     6601 atgtgagatc aacaaggcga ttatcgcaga gaatgttgtg gtaggtaatc aggtaaaatt
     6661 aggtgtcggt gaagaagcag agaacgatac ggcacctcat atttacaacc acggacttgt
     6721 aaccatcggt gagaagagcg tgattcctga cggtatcagc gtaggcaaaa actcagtgat
     6781 atccggagtg acagctgctg cagattatga ggattcccag ctcgcaagcg gcaaaacatt
     6841 aattaaggca ggtgaatagc agtgagagcg attggaatta ttttagcagg cggtaacaat
     6901 cacaggatgc gggaactttc ccagaagaga gctatatgtg cgatgccgat tgcaggcagc
     6961 taccgcagta ttgactttac actgagtaat atgaccaact ctcatattca gaaggtggca
     7021 gtgtttaccc aatacaatgc aagaagtctg aacgagcatc tgaattcttc caaatggtgg
     7081 gatttcggac gtaaacaggg tggattatat gtattcactc ccgctgtaac agcagagaac
     7141 agcaactggt acagaggaac cgcagatgca atttaccaga atctgcagtt tttgaagaac
     7201 agccatgagc cttatgtagt tatcgcatcc ggcgactgtg tatacaagct ggattataat
     7261 aaggtgctgg aataccatat cgagaaaaaa gcagatatca ctgtagtctg ccgcgatatg
     7321 gaagccggta cgaatgtaga gcgtttcggt acgattcgaa tgaacgagga aagtcgtatc
     7381 gaagagttcg aggagaagcc cttacaggcg aagaccaata ccatttcctg tggtatttat
     7441 gtggttcgac gcagactgct gatcgagatg atcgagaagt gtgctgagga agaccgctac
     7501 gatttcgtaa gagatatcct gatccgttac aaggatctga agagaatcta tggatacaag
     7561 atcaaggatt actggagaaa tatcgcatct gtggatgatt actacagcac caatatggac
     7621 ttcttgaaag cagatgtgag aaactatttc ttcaagcagt atccggacgt atattccaag
     7681 gtggatgacc tgcctcccgc aaaatataat cccggttccc aggtgcgtaa cagcctggta
     7741 tccagtggat gtatcgtcaa cggtgtggta gagaattccg tgatctttaa gaagagctat
     7801 atcggcaata attgtgtgat caagaattcc attatcctca atgatgtgta tattggagat
     7861 aacacgcata ttgaaaactg tattgtagag agccgtgaca cgatccgtgc aaattcgtac
     7921 tttgtgggag aaggcgaagt taagatcgta gttgagagga atgaacgata cgtgatctaa
     7981 gtgatccgga ttctcaatga ctacattgcg agactgcttg caggcagggc aatgtggtca
     8041 ttagggaatc taacagatat gagtaagtag ggcgatagcc cggaatacga atatctgtag
     8101 cgtttcgaga gcctgcaggg cggagaaatg cgtagggatc acgataacac caagggggtt
     8161 agcaaatgca gattacagat gtacgtgttc gtaagatcac caaggaaggc aagatgaaag
     8221 ctatcgtatc cattacactg gatgatgaat tcgtcgtaca tgatatcaag gtgatcgaag
     8281 gtgaaaaggg attgttcatt gcaatgccga gcaagaaggc aacggacgga gaatacaggg
     8341 atatcgccca tcccatcaat tcttccacca gagagaattt gcaaagggtg atcctggaga
     8401 gttacgaaaa agcattggag gagccggaag aggcaatcta agctacatag agataaacaa
     8461 caaaagctgt tgccaacaga gtgatctgtg gcaacagctt tttaaattgc agttatcttt
     8521 tattccccgg acgaaaatca ggaacgctga taagcacata atccgttttc aaattgcaga
     8581 cgatcgatca attccaggga accgccgatt cgccggtata gttctatggt gcctgttgca
     8641 ttgccgcctt cccagagagg aatgtcggat acttttccgt caggactttc atagagtaac
     8701 ggcatcatct gctctttgct gcagcttccg gagatccgta caacggaagt gcggttctgg
     8761 gctttgaaat gccagataaa acgtttgttg gtctctttca ctttccattt gcagcgggac
     8821 agtgtaagag gtctgccaaa atgatactca aaatcttccc cggtataggt cagctgcatc
     8881 agaatcctgc gtcgcatggg gaaaaagaga aatttcggac aacagccgtc gatggccaga
     8941 gccgaatgct tcaattcccg cccggttttc tcgctgatca ggtggccgga agcaaattgc
     9001 agccagggct ggttgtaact gcggccccaa tgtttgtccg catagccgta ggaggtctcg
     9061 ggagttacct cgtaggtgac accgttcaaa atgatggtcc cacggaaaaa ggtacggata
     9121 ccttccccat gccagaaact ttccagggca tgaaccgcgg taaaaaaagc attggcaata
     9181 tagccggtat gacaggcaac cgccttatgc acttccagat cccagataaa actgcccgca
     9241 tcggtcataa gagagcgatg tcttgcaacc tcatcggaga tatcaatcga accactgatc
     9301 cggttttcgc tgtagacaca atcctcaaac tgcatgtaaa agggatcctg ggccacctgc
     9361 agagaggtca ggggataata tgcctgaagc tggagcccgc tatctcccgg ctcagggaat
     9421 acaccggctt ttatgcagag ataagatggt ttcagaccat gacgtttata gtagggatgc
     9481 tgacctaaaa taggctggga agtaccgaga gccggattta aaatgctgta ttctacaaaa
     9541 aaagtgcggg tttcctgcgt cgtgggacaa atgccctgga aggaatgcca ccagcgcatg
     9601 tagccacgtt ttgcatcgga accccgcaac atatatgcat ttcttttcag atcagataaa
     9661 ttcatggatc aaagactccc ccgcttttgt acccctcaaa acaacatttc gtagataata
     9721 tgtatattat agtactatat gttgtgaatg acaagagtgt cggaagaatt ttaagaatta
     9781 tttagatatt ttctttgatc cggatatcta ccgctacata attgtattct ttttccggat
     9841 tttcgccggt cgtcagatag gcaaacagca gggaaagagg tttgctgccc tgaattttcg
     9901 gctgttggca gatggttgcg gcaattgtgt cattttccag catttggcgg gtggtgggga
     9961 ccagatcaaa agcgatgatc cggatcgcct tttttttacc aagagcctct acactgcggc
    10021 agcctccgta gacaccgccg gcggcgaaga acagtgcatt gatctccggg tgctttgaca
    10081 gaagtgtcgt tgtcttttcg tagctttcca cttcgtcatc gtggatctcc acggtatcca
    10141 cgacgcggat ccggtcagag aaaggttcta atgtcttcag aaatcccgca atgcgctctg
    10201 tgtgacagag aatgttggag gaacctgtga caatacccac gtttaccgtg ccggaggtca
    10261 tcaattgcag aagaccggcg gcggtggcac cactgcgggt gtagttgctg ccgacataag
    10321 cgatacgtct ggaattttca atgtctgtat tcagcgtgac cacgggaatc ccctgctcgt
    10381 ataaagtatt gatgcagttg cggatccgtt cgtcgttgta aggggtcatg gcaatgccgt
    10441 tcacttcttc tgcaagaagc tctttgactg cctgtaactg ggcctccaca ccgaaagaaa
    10501 tctgtttggt gatcacggta cagttgtaac ctgccagctc ttccgccttt tcattgattc
    10561 cggccagaac atcctgaaaa aaaggattcc cggtggaaaa cagaatgaca cccagcttca
    10621 gccgcttttt ctgcgctgcc agaacgagac cggcgcggtt gggtttatag tccagctctt
    10681 ttgcaatttt tctgatcttt tcggcggtct ccgggtttac tgcgccgcga tcgtttaaga
    10741 cgcgatccac agtgccgcgg gatactccgg ccaatgcggc gatttctttg atggttgcca
    10801 tagaatactc atgcctttcc gtaatatctg ttccaagcat ctggttataa aagtatcata
    10861 tttccagagg gaagtcaacg aaagtgcaga atttgtccgg gcacgctgaa tggatgatga
    10921 atgatgaaat tccatcttca ctttttagtg aaaaaatact gaaaacataa aaacttctac
    10981 ttgaaaaatt ttttcacaac agttatgata agactataac gtgcacgggc acgaaaagga
    11041 acgaaaatgg atcacagcac aaacgtagga ggaagattca tgctatatat tggaatcgat
    11101 ctgggcactt cggcggtaaa actgctttta atggacagtg aagggaacat ccggaatatt
    11161 gtatcccggg aatacccttt gtatttccca catcccggct ggtcggaaca gaagccggag
    11221 gactggtatg aacagactat ggagggcatc cgggagcttt tgaaagatgc agacaagagc
    11281 caggtggcag gcatcagctt cggaggacag atgcatggac tggtcattct ggatgagaag
    11341 gatcaggtga tccgccctgc catcctctgg aacgatggca gaaccacaga ggaatgcgat
    11401 tatctgaata atgtgatcgg caaagagaag ttgtctgagt acactgcgaa tatttctttc
    11461 accggattta ccgcgccgaa gatcctctgg gtgaaaaaga atgagccaga gaacttcgca
    11521 cggatcaaaa agatcatgtt gccgaaggat tacatagctt atcgtctgac cggcgtacat
    11581 tgtacggatg tatcagatgc ttccgggatg ttattgtttg atgtgaagaa ccgttgttgg
    11641 tcgaaggaaa tgtgtgagat ctgcggagtg agagaagagc agttggccac ttgttatgag
    11701 agttatgaaa aggtgggctg cgtattgccg gaggtgtcag acgaactggg gattcccgca
    11761 aatgttgtgg tagcggcggg agccggggat aatgcggccg ccgctgtagg taccggaact
    11821 gtaggagaca atatgtgtaa tatttccctg ggtaccagtg gaacaatctt tatttcctcc
    11881 aatcatttcg gagtagatca gcacaatgcc ctgcattcct ttgcccacgc agacggacat
    11941 taccatctca tgggttgcat gctgagtgcc gcttcctgta ataaatggtg gatggatgag
    12001 atcatcggaa cgaaggatta tgcctcagaa caggcgcaga ttgagaagct gggagaaaat
    12061 catgtctact tcctgccgta tctcatgggc gagcgttctc cccataatga tcccaatgcc
    12121 agaggaactt ttatcggaat gaccatggat accaccagag ccgatatgac gcaggcggta
    12181 ctggaaggcg tggcatttgc actgcgtgat tcactggagg tggcaaaatc tctgggaatc
    12241 catctggagc ggaccaagat ctgcggtggc ggagccaaga gcccgttgtg gaagaagatg
    12301 atcgccaata tcctgaatct gaaggtggat gtgatagaaa gcgaagaagg accggccatg
    12361 ggcggtgcca tgctggcggc agtagcctgt ggggaatatg caagtgtgga ggagatcgcg
    12421 gagaagttcg tcaaagtcac cggaacggta gaaccggatc cggaactggt ggcaaaatat
    12481 gaagaccgct atcagaaatt ccgccagatc tatccggcat gcagaccgct gtttgagatc
    12541 attaaataac gcgtagaaaa atcaaacaac aacataacaa aatgaacctc gtaattttaa
    12601 gtttgagcta taatagagaa aggttggtag agtagaatgc atattttatc tgtaaaagat
    12661 cctgcatttc gtaagtatgg cagagtgatc accaatgtgg attttacaca gctggtggaa
    12721 gaaatgaaaa agactccggt tcctgacggc gtggtgtatg agcccgggat caatgctttg
    12781 gaagcattac ctgtgaaaaa agaactggag accatcacat acggagaaat gcctgtacag
    12841 atcggttact gcaatggcca taattccaaa ctgaatgccg tggaatatca tagaagttcc
    12901 gaaattaatg tggcagcaac ggatgctatc ctgatcctgg ggctgttagc ggatgtggca
    12961 gaagatttta cctacgatac ttctaagatg gaagcatttt tagtaccggc cggaaccggt
    13021 gtagaggtct tcgcaacttc tctgcattat gctccctgtg gagtagacgg acagggattc
    13081 caggtagcca tcgtgttacc tcagggcact aactatccgt tagaaggagc acatcagaag
    13141 gtggagcagg gcaaggcacc cagcgaggat gcgttgcttg ccgctacgaa caaatggctg
    13201 atcggccatg cggagggtgg acttccagag ggaagcttcc tcgggcttgt tggagagaat
    13261 ctggatgtca gcaaataatg gtggcagaat aaaaagattt gaacgccgga atcccctgca
    13321 tggatgtatg acggcggtaa aataaatggt gtagagaaaa agaaaacgaa aataggagga
    13381 ttacaaaatg agtaatttac ccgaagtaaa aattggcgtt gtggctgtca gccgtgactg
    13441 tttccccgag agtctttccg tgaacagaag aaaagctctg atgaaggctt ataccgagaa
    13501 gtatggaaca gacagtgtct atgagtgccc ggtatgtatc gtagagagcg agatccatat
    13561 ggtacaggct ctggaagaca tcaagaaggc aggctgtaat gcactgtgcg tatatctggg
    13621 caactttggc cccgagattt ctgagacctt actggcaaag cattttgacg gtcctgcaat
    13681 gttcattgca gcagcagaag agacccagaa tgatctgcta ggcggcagag gcgatgctta
    13741 ctgcggtatg ctgaatgcaa gctacaactt aaagcttcgt aatgtaaaag cttatatccc
    13801 cgagaatccc gtaggaactg cagcggaatg tgcagatatg ctgcatgagt tcatttctat
    13861 cgcaagagct attgtgggat taagtgatct gaagatcatt tccttcggcc ccagaccttt
    13921 gaacttctta gcttgtaatg cacccatcaa gcagttgtac aatctggggg tagagatcga
    13981 ggagaactcc gaactggatc tgttcgaagc cttcaagaag catgatggag acgagagaat
    14041 ccccgccaag gtaaaagaga tggaagcaga actgggggca ggcaaccata agcccgaagt
    14101 actgcctaag ctggctcagt atgaattgac actgttagac tggatcgagg ctcacagagg
    14161 atatcgtaag tatgtagcca ttgccggaaa gtgctggcct gcattccaga cccagtttgg
    14221 tttcgtaccc tgctatgtga acagccgtct caccggtatg ggcatccccg tatcctgtga
    14281 agtagatatt tacggctgct taagtgagtt catcggaacc tgcgtcagcg cagataccgt
    14341 aactctgctg gatatcaaca acactgttcc cgatgatatg tacgaagagt ccatcaaggg
    14401 taaattcaac tatacccaca atgacacctt catgggattc cattgcggta acaccaactc
    14461 caagaagctg acctcctgca acatgaagta ccagatgatc atggcaagaa cccttcccga
    14521 agaggtgacc cagggtactc tggaaggaga tatcgttccc ggagacatca ccttctatcg
    14581 tctgcagtct accgcagaca acaagctgcg cgcatatatt gcacagggtg aagtacttcc
    14641 tgtagctacc agatccttcg gtggtatcgg tattttcgct atccccgaaa tgggacgttt
    14701 ctatcgtcac gtactgatcg agaagaactt ccctcaccac ggtgctgttg ctttcggaca
    14761 cttcggcaag gctctgtacg aagtattcaa gtacatcggc gtacctgtag aggatctgaa
    14821 tttcaatcag ccgaagggaa tgctgtatcc taccgagaat ccttttgcat aaaaagaact
    14881 taagtaactg gaaagttagt taaatacatt ggcaaaatcc acgaactccc ggagcatgcg
    14941 atacacatgt ctcgatgtaa ggtctccgga tgtgaatttt ctacgaaaag tgatgagtag
    15001 ttctcgtcat gacggggccg cctacagtcg acatccatgt cgacaatcgg ctgacgcgga
    15061 catcgtgtcc gctttccccc tgtctttcag cacttttcta gaaaatatca acatcctccg
    15121 acaaacatct tcgacatggc accgcatacg ccgggagttc tacgttttgg aaagctttca
    15181 aaaaccagta gccccggctg ggtataagaa taagcagtgc cgtaagcaga cgtcggtact
    15241 tagcggtcgt actgtgaccg cttatgtacc gctacgctct gcgacagagc gcgagcgtgc
    15301 cgcgtttctt ataccctgcc aagggctacg gggatatttc gctaaattgc tatttagcag
    15361 gagatttctt ccgggctttc acaacataca caatggcgat caccacatcc gcacagtaga
    15421 taaaacatcc gatccccatg aagatggcac ccggcaggga gacaagcctt tctttgcgca
    15481 tgcgaatact gcagccgatg gaactgatgc cggtgagcag gatcgtcaaa agatcaatga
    15541 gtactgccca caggagaaag ccgatgcccc agacactcat ggcaagtccg atgaaaccca
    15601 ggataaagtg catgatataa gcaggaatct gaattccttt gatgatcagg ttcatccggg
    15661 cagcctgccc tgcagtcaat tttcccttgg cggtctgtac cgcattgacg attacaatga
    15721 caaaggaata caggttatag atgactgcga tgataagcag gacaagcagg gagatatccg
    15781 tggtatcctc tggcagtacg cctacaaaca ggaagaagac agcaagaatc aatacataaa
    15841 tataagaata gataattaca ggtatcattt tcagatattt caaaaattcg ttcatgacaa
    15901 ttccactctc ataataacaa tgcttgtaaa aaaatgacaa aaagagtata gcaaaaaaac
    15961 ataaaatgtg caaaaaaaga tattgctttt ttggagcgcc gcgtatatac tctcaacgat
    16021 agaaataaca ggaaacactg ataaattcag tgcttgcggt tgatttgaaa aatggagagg
    16081 ttattaccat gacaaaagac ctgacaaaag gcagccccat gaaacttatc atcagctttg
    16141 ctgtgccgtt attattcgga tttttattcc agcagttcta taatctggta gataccatga
    16201 tcgtaggacg gtttcttggt gtggatgatc tggcagcagt aggctctacc ggctccatca
    16261 acttcctggt catcggtttc tgcatgggtg tctgcaatgg atttgccatc cccgtgtccc
    16321 ataaattcgg tgccggagat tatgtgggca tgcgcaaata tgtggccaac tgtgcctggc
    16381 tgggacttgc cttttccgtc atcatgaccg tggtgacggc gatcctgtgc cgggacattc
    16441 tggtatggat gaagacgccc gccaacatca ttgacggggc gcataactat attttcatta
    16501 tatttatcgg tattcccacg gtattcctgt acaacatcgt ggcaggcgtg atccgtgcaa
    16561 ccggcgacag caagactcct gtggtattct tagtgctgtc ttccattatt aatattgtgc
    16621 tggatctgta ttttatcata tccctgcaca tgggcgtggc gggggcagcc tgggcaacgg
    16681 tgatctccca gggagtctca ggtgtggcat gtctggtcta tatggtgaaa aaattcgaga
    16741 tcctgcgaat ccacagggaa gaatggaaag tggacggaca tatgatgatg gtgttgtgcg
    16801 gcatgggagt tcctatgggg ctacagtatt ccattaccgc cattggcagc gtaatcttac
    16861 agactgcagc caacacctta ggttctgcgg cagttgcggc catgactgca ggcggcaaga
    16921 tcggtaactt cttagcctgt ccttttgatg ccatgggttc cactatggca acctacggtg
    16981 gacagaacgt gggagccggt aaactggacc gtcttggcaa gggacttaaa tcctgtgtac
    17041 tgttgggggt aggatactct gtgatcgcat ttttgattgc agtatttttc ggcggtccgc
    17101 tggcgcagct gttcgtggac ggcggcgagg cagagatcat tgccaacgtg tgtgctttcc
    17161 tgttatggaa tacggcattc tatattccgc ttgcactggt gaacattgtc cgtttcctga
    17221 tccagggtat gggtttcccg aaattcgcca ttttagcagg tgtcttcgaa atgatcgcca
    17281 gatccctggc aggctttgga ctggtgccaa tcatcggctt caccggggta tgcctgggca
    17341 gtcctattgc ctggatcatg gcggatgcat tccttattcc ggcttatttc catgtgagta
    17401 aggtgctgca aaagaggatg gtgccgcaaa cggatgtccc acaggcagtt tgacgatacg
    17461 ccggtcgtaa gtcaccagac cgttcacctc ttcttccaca tcggagagct gcgtatatac
    17521 cgcaccgctc aagccctttg agatcagagg aaataattgt ctggaaaata actgctttag
    17581 ggcagcagcc atctcctccg gggtgtcata tttctgatat ccgtagaccc ggtccacact
    17641 ggaatgcccg tcgatgtgac aggcgtagcc accatattcc gagataacga aagccctttt
    17701 tccatccaat tttacctgta acggacggaa gtagttatga atactgcgga aatccccgcc
    17761 tttctggtcg aaccagccgc tggcatggtc taccggacgg gtggggtctt tttttgccac
    17821 cattttcgca atacgggcag catcgaactg tccccagcct tcgttgaagg gcacccatac
    17881 ggcgatggaa gggacattgt acaggtgatc tatggtgtca agacattcct gttcccagct
    17941 tttccggcct tcttccgaac cacgggagaa cagctcataa tgattatcct tcgtgtgggc
    18001 ggacatgtgg gggaacagtg tgggcatata gcatacccag ggcatatgat aggtagagcc
    18061 gccgctgacc atatcctgcc atacgatcat tcccagacga tcgcagtggt aataccagcg
    18121 cagagactcg agcttgatat gtttccggag catattgaag cccagccctt ttgcgaggct
    18181 gatatcatag acaaaggcct catcgcaggg agcagtcatc agcccgtcgg accagtagcc
    18241 ctgatccagg atcccgtgga gaaaataggg cttgtgattc agacaaaacc gcaaaattcc
    18301 cttggaatcc ttttcgactg agaaacatcg catggcaaaa taaccatcca cggtatcctc
    18361 atcggcgttc agggtaaaag gatacaacca agggtgttcc gggctccagg gcttgaaatt
    18421 taccggctca ggtgcatcag cgggatccgt tgcagaatag gtgacgctgg cgggaaaaga
    18481 aagcacgacc gtggtatgcg tgagcccaaa ggaatcctgt tgctcagaaa ttttacgtac
    18541 acgcatccgc gtgagtgaac cggtattttc ctgcgcgagg gttccgtcgc aggagggggc
    18601 acttatccgg aattccagat ggttgaacgg cttcggagaa cacagaacga aggttacgga
    18661 agccttgttg tactccgggg tgatcttata attgatcaca tagtttttcg gaacccattc
    18721 gtaccagaca ctctgccaga tgccgctctg ggcggtgtag aacatgccgc cgcggttcag
    18781 ggtctgcttt cccctggtgg cgtaaccgct gtcggtcaga tctcggactt ttacatagag
    18841 agataccgga tctgcgttta catattctgt gatgtctaag gaaaaaggaa ggtagccgcc
    18901 actgtgagtg ccaagcttct ttgtgccgag gaaaacggtt gcttcctgat ctaccgcgtc
    18961 aaaatgcaga atgcaacgca tattttcctc tttttgggaa agctcttccg tggaaaaaga
    19021 aagttcccgg tggtaccaca ggtactccgt gggttccagc cgtctgtgaa ctccggaaag
    19081 cagggcttcc ggcgaaaaag gcacccggat ggaaccctga gcggtaaaat acgcagaatc
    19141 ttccgaagaa ctactcccat ctgcgggcac aaaagcatat tcccagagac cgttcagcat
    19201 atggtaatcc gttcgtttca tttgcggacg gggatattcc ggcaggatat cgtcatagga
    19261 accggatcgc accagcgatt ccccccagag agtatagagg ggatgcagga tgtcttccgg
    19321 gtgcgcctga ttcagattcg cagcagcatc ttttaagttc atgcgaaaac cttctttcct
    19381 aaaatagatt atttatagta tactacattt ggaagtatag cacatgaaaa atgatacgat
    19441 tctttgagaa aaaagttcgt ataagctatg cgtaaattct aaaaagaggt tatacagatg
    19501 aatatactga ctgttgacaa tattaccaaa agttatggag tacgcaagat ttttgacgga
    19561 gcctcctttt tcctgcagga aggggaaaag gccggaatca ttggtattaa cggaaccgga
    19621 aaatccactt tgctaaaaat gattgccggg ttggaagaag ccgatgatgg caccgtgatt
    19681 aaggcaaatc attgtctggt gcgttatctg ccgcagaatc cggagtttgc tccggaggag
    19741 acggtgctgg aggcagtgct gcgtggcaat cgtgtggagg aaaacgccgg aaccatcgag
    19801 gcggatgcca aaagcatgct gacgaaactg ggggttacgg aatttgatca gccctgcggg
    19861 cagctgtccg gcggacagcg caagcgtctg gcactggtat cggtactgtt gtccccggca
    19921 gagatcctgt tattggacga gcctaccaac catctggaca attccatgtc tgactggctg
    19981 gaggatcagt tgcgccgccg caaaggcgct atgatcatgg ttacgcatga tcgttatttt
    20041 ctggacagtg tcactgaccg gatcctggaa ctggacaaag ggaagattta ttcctatgat
    20101 gccaattatt ccggctatct ggaactgaaa atgcaacggg aggagatggc acaggccagt
    20161 gagaaaaaac gcctgaatat cctacgcaat gagcttgcct gggtacagcg gggcgccaga
    20221 gcccgctcta cgaagcagaa ggccagattg cagcgttttg aggaattgaa aaatgtgaag
    20281 ggacctgtga cggacggcaa agtggaactg acttccgcag catccagact gggccgtacc
    20341 acagtagaga tcgagaatgt atccaaaagc tatggcgata aagtgttgat ccgtgatttt
    20401 acctatctgt tcggcaagga cgaccgggtg ggatttatcg gccctaacgg cagtggtaag
    20461 accacgctga tgaaaatgat cctgggtgaa gtggctccgg acagcggaca tattgagatc
    20521 gggcagaccg tgaagctggg atattatgcc caggagatcg acgacaccat gatgcatccg
    20581 gagcagagag tcattgatta tatccgggat gtggcagaat atatcaccac ggaggatgga
    20641 cagatcacgg cagcgagaat gctggagcgg ttcctgtttt ccggggaaga ccagtacgga
    20701 cagctgggaa aactgtccgg cggagagcgg cgcagactgc aattatgcaa ggtactgatg
    20761 ggggcaccca atgtcctgat tctcgatgag cctaccaacg atctggatat tgccaccctg
    20821 caggtactgg aggattatct ggataccttc ccaggcatcg tcatcgtcgt gtcccatgac
    20881 cggtattttc tggatcgtgt ggtccgcagg atcttcgccc tggaggatac ccgcatggtg
    20941 cagtatgagg gcggttacac agattatgcc aataggaaag ctgcggaggg gaacacagag
    21001 agcacaggaa cggcggcagc atcccagccg aagaacgccg atgaggaaac tacttctaaa
    21061 aactggaaaa acggacagaa acgcaaatta aaattcacct accaggaaca gaaggattat
    21121 gaatccatcg aaggggaaat ggcggcatta gaggataaaa ttgcaacttt ggaagcagat
    21181 ttttcaaaat atgccagtga ttttgtgaaa ctcaatgaat taacacagga aaaagaggct
    21241 gcggaacagc aactggaaga aaaaatggaa cgctggatgt acttagaaga cctggcggcg
    21301 agaatcgaac ggggagaaga aaactgacag tccgaagtcc ggaatgtcag tcttctgcat
    21361 cgtcggtagc tgccgcaact gcggatacca cggaggatac tacggcgatc actactgcca
    21421 aaaggatgat caaaagtccg ccggctaaaa gtaaaaacat aaagtacgct cctctctgaa
    21481 ataactttac agacacccat attatatcat ttttcctaaa aaaaacaata aaaatcggca
    21541 tatagtagaa aaaatcacca aaatgaagta taataaaagc attcatcggg actgatttat
    21601 gttttgagga gggtgcctat gacatatcag aaagcaaaag aggaaatcgt tgaatcttct
    21661 ccgcagaaaa cagatgccgg aaaagaagaa ttatatctgt attctcttag aaaaatggcg
    21721 gaagaaaatg tagaaaataa cagaacaggg ctgacaaatc tgcataatat caattctttt
    21781 ttctatctgt gcggtgaaat gatcaagcag cagccggata aaaaatacgg tgtcataatt
    21841 atggatattg tacagtttaa ggctgtgaat gagttctgcg gcagagacga aggagacaga
    21901 ttgctaagat ttattgcgtc ttgctttgac tggtatgaga ataacaggcc ggatagctat
    21961 gcctgccata tcagagcaga tatcttttgc ttatgcacaa gctatgagga agtagaggaa
    22021 ctggaaatta ttgtgcggga gatcagaaaa aagataactg actttccgtt cgcttaccgt
    22081 gtacaaccat cctttggaat cggaatctca ccggagagag cccctgccat cagctatctg
    22141 aaagattgcg ccacgatggc tatgaacagt atcaaaggca aagtatatcg gacatatgca
    22201 gtatttgacg aaaaaatgcg tagtcagaag atgcgagaac gccaggtgga aaatgatatc
    22261 gtgtccgcac tggagaacgg agaactacaa ctgtatgtgc agcccaaggt cgatatgagg
    22321 gacggaagag tgatcggagg cgaggcgctt gtgcgctgga agcatccgga aaaggggctg
    22381 gtgccaccgg gagagtttat tcccgtactg gagaaaaacg gatttatcat caatgtagat
    22441 gaatatatct gggagaaagt gtttgcttat cttggaaagc tgcgtaagga aggccggaca
    22501 cttattcccg tttccatcaa tgtatcccga ttgcatgcgt atgatgagaa actgacagag
    22561 acactgctcc gtctcaggga ggaatatgat gttctgccgg agtatgttcc gctggaactg
    22621 acagagagcg cattcctgga ggacgaggtt ggtatgtatc gccgcatgga aagcctgcgg
    22681 gagcgagggt tcctggtgtc catggatgat ttcggtaccg gatattctac tatgaacatg
    22741 ttaaagaatc agacattgga tgagattaag atcgatcggg aattcatccg ggatctggag
    22801 aaagataaga gtcagatcat tatccgcaat accattgcca tgctgcagca gctgggagca
    22861 catatcgtca ttgaaggtgt ggagacggaa gaacagaagg agttcctgtt aggctgtaat
    22921 tgtaccgatg tacagggctt tttattctac cggccgatgc cggtggagga gttcgacaaa
    22981 ctgctttggc agcaggaacg ggagctggat atggcaaaat agacaaagac aaaatagaca
    23041 aatgcgcaga ccgcattctg acgggctttt tgaaattaac ctgacagaat gcggtttttc
    23101 tttggccatt cttccgaaaa tgagaaaata gttccggagg cgttgacaga tgccacggaa
    23161 gggagtaatc tgcatattac ccgacaggtc agtcgaggtc aaaattctaa atatcaggtc
    23221 atattcgacc aggaatcaca taatgtatta attagtgcaa aatggattat tttgagaaaa
    23281 accaacagga ggatgtatgg aggaaagaga aaacaaaagg gaagaaaccg cctctgatgc
    23341 agaattgcag aggctggtag aagattttat cacacagaaa caggtgcttt tgatccaggt
    23401 aaaaaagggt gtgttgggca aggaggaatt tctgcaggag gcaggaaaac atatcgatca
    23461 atattatcat ttcccggcaa cgaaaaggaa aagactttta aaatcatttg aacaatatat
    23521 tttcggttat tccagactgt caccgctcat ggatgacaaa agcatttcgg atatccgggt
    23581 ggtgagtcat gactgtatcc ggatcaagag agctgctaaa cgtatggatg ccggaaatgc
    23641 ctttgcttag gagctggagt accggcaggt tatagat
//
LOCUS       DAWDHD010000023        23270 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_164_length_23270_cov_10.831833, whole genome shotgun sequence.
ACCESSION   DAWDHD010000023 DAWDHD010000000
VERSION     DAWDHD010000023.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 23270)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 23270)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..23270
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_164_length_23270_cov_10.831833"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    21170..21269
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         6741 a         4589 c         6297 g         5543 t
ORIGIN      
        1 aaacgggtgg agcaaacacg atgtttgcga cagccgatcc gcactacgga tggtgcgtgt
       61 aggcgtgccc gtgttaagta gaacaaatga atcacttttc ctagaaaatt cacactcgga
      121 gaccttacac cgaggcatgg tatccgtacg cttcgtgaag taacatagaa aggtgaattt
      181 catgaacgaa atggaacaga aggttctaaa gaccctctca caccaataca aaaatatagc
      241 ggcagcctcc acagagatca tcaacctcca gtccatcctg aacctcccca aaggcaccga
      301 acatttcctg acggatatcc acggagaata cgaacagttc aaccatgtcc tgaaaaacgg
      361 ttccggttcc gtacgccgca agatcgacga ggaattcggc aacaccatca gcaacaagga
      421 caaaaaaagc ctggcaaccc tgatctatta tccagagagc aaactggaga tcgtggaaag
      481 agaagaggac aatctggagg actggtacaa aatatccctt catcgtctgg tacaggtcat
      541 caaacgtgtc tcttccaaat acacccgctc caaagtcaga aaggcattgc cgaaggattt
      601 cgcctatgtc atcgaagaac tgatcacgga gaaggaagaa atccaggaca aagaggcata
      661 ctataatgag atcatccata ctatcatccg catcggcaga gcgccgcagt ttattattgc
      721 gttaagccac ctgatccagc agctggtcat cgatcatctg catatcgtcg gtgatattta
      781 cgacagaggt cccggccctc atattattat ggatactttg tgcgaatacc attccgtgga
      841 tgtccagtgg ggcaaccatg acatggtgtg gatgggcgcg gcggccggaa gtctggcctg
      901 tatcgccaat gtggtcagaa tgtctgccag atacggtaat ctcgccactc tggaggatgg
      961 ctacggcatc aacctgctgc ctctggctac atttgcactg gaaacttatg agaataccaa
     1021 ctgcgatgcc tttaccatca agttcaatac cgattacaat accaaggatc tcgggctgga
     1081 caccaagatg cataaagcaa ttgctatctt gcagttcaag ctggaaggac agctgatcat
     1141 gagacacccg gagttccata tggaagaccg gatgctgctg catcggatcg atttcgagaa
     1201 aaagacgatc tgtgtggacg gcaaagaata tcccatgaaa gacgtggatt tccctacggt
     1261 ggatcctgta catccttacg aactcacgga ggaagagagc aaggtcatgc tgcgcctgca
     1321 gcaggtgttc atgcgctgtg agaaactgca gcgccatgta aaatttctgt tttccaaggg
     1381 cggcatgtac aagatttaca acggcaatct gttgtatcac ggctgtgtcc ccttgaatcc
     1441 ggacggcagt ttcatgcagg tggagatctg cggaaaagag tattgcggaa aagcactgta
     1501 cgacattctg gaatattacg ccagaagagg ttattatgcc aaggaagcca aagagcgcgc
     1561 cctgggacag gatatgatct ggtacatctg ggcaggcccc ggttccccgg tgttcggcaa
     1621 ggcaaaaatg gctacctttg agcggtattt cctgaaggat aaggagactc atatagaaga
     1681 gaaaaacagt tactataagc tgttggaaaa tgaggaagtc atcggcagaa tcttagagga
     1741 attcggtctg gacaaggccg atgcccatat cgtcaatggt catgtgcccg tggaacagat
     1801 ccatggggaa tcacccatca aatgcggcgg taagctgctg atcatcgacg gtggcttttc
     1861 caaagcatat cagaaaaaga cgggaattgc gggatatacg ctggtatgta attccagagg
     1921 aatgcgtctg gtagcccatg aaccttttga atctacggaa gcggcgatca tcaaggaatc
     1981 ggatattttc tcggattccg tggtggtgga gaatttcccc cgcagaaaac tggtggccga
     2041 taccgatacc ggcaaggaga tccaggagaa tatcttctat ctggaagaac tcctggaggc
     2101 ttaccgggaa ggtacgatca tggaagaggt ataacttatc tctggccggc tccggtggtg
     2161 gcagtggtag aattgttgtt acctgcagta tcattaccgt tgcctaccag gtcgttggtg
     2221 atatcggaga caccgtttgc ggcatcgtcc acgatattgc ctacggtatc accggcttct
     2281 ccgatggcat tgcctgcatt gtccagcatg gaatcatttt ctcctacggt gttaccgttg
     2341 ccgttcatgt cacccatagt gctgtcggta gcggcagttc cggtctctgt ggtctgcgcc
     2401 tctgtgctcc cctgagcagc aggagaggtg gacggagaag gagaggcagc catgctgccg
     2461 gagttattat tgttgttctt gttggtgcca caggcggtcg ccatacacag gtagactgtg
     2521 atgatgccca gaaccagtaa ttttttcccg atgtgggatg ttacgttatt tgtcttattt
     2581 ttcataatgt cctccatttc aagatgcccg tcagggatat tcagtccgaa ccaaaggtgg
     2641 tcggcttgag ggtagtatgt gcggatttta gaaaataatt caaaaaaata aaaatcattt
     2701 ggaagaaaac gaggaaaaag atggaatttc gcccttgtat tgatattcat aacggaaaag
     2761 taaaacagat tgtcggtgga agtttaaaag atctcggtga tcgggcgcag gagaactttg
     2821 tctcagaaca ggacgctgcc ttttatgcaa aactgtatca gaaaaacagt ttaaaaggcg
     2881 gacatattat tttattgaat gcctcggaca gcccttatta tgaggcaacg aaggaacagg
     2941 cattgtcggc cctgcgtgta tatccgggag gattgcaggt cggaggaggc atcacggcgg
     3001 agaatgcggc agagtatctg gcagcagggg cctcccatgt gatcgtgacg tcctatgtgt
     3061 tcagggaggg acggatcgac tatgacagac tggacaaact ggtccagtcc atcggaaaag
     3121 agcaccttgt gctggatgtc agctgcaagc gtgcgggaga gcagtatctg gtagtgactg
     3181 accgctggca gaagctgaca gaagaagcag tggatgaggc actacttaaa aaattatctg
     3241 cctattgtga tgaattcctg atccatgccg tggatgtgga aggcaaggcc cggggaattg
     3301 aacaggaact ggtaaagttt attggaaatt ataccggaaa tccaatcacc tatgcgggtg
     3361 gtgttcactc catggaagat ctgtatttgt taaaaaaact ggggcagaac cggatgaatg
     3421 tgaccatcgg gagtgcactg gatctcttcg gcggcagcat gaaatgggaa gatgtgttgg
     3481 cagtatgcag agaaaaatag gagtggaaac gaaaaatccg caggtgctat catacgatag
     3541 cgcttgcgga ttttggttgt ctgttatttt tcgtttatga ttaaagcttg gttacgttaa
     3601 cagcctgagg tcccttttct ccatctacga tatcaaaaga aacggctgcg ccctcttcca
     3661 gggtcttgta accgtccatg ttcagaccgc tgtagtgtac gaatacatcc ttgccggatt
     3721 catcggagat gaaaccataa cccttctgat tgttgaacca cttaactgta cctttgttca
     3781 taatagatac ctccacaaat tctttgctgt catttacttt ttgcaatgta tacaacattg
     3841 tctcagcaag tgcatcatag cacagcaggt agtaaaagta aatagacgat tctgtaaaat
     3901 tgcacaaaaa tttataggca atttcctttt aaatacgaat aaatgaagaa aatcttggga
     3961 aaatattaca aaaatgttaa gaaatgattg ctgtaagagc tttcctgtgg taaaatgggt
     4021 tgatatttag cggaggtgac tctatgaaaa gacgtaaaca taagcgaaga ttgaaccatg
     4081 tgctgattct tacgtcagac gcagcagatg ccaatgtaaa gcagttccgt gtacgtgcat
     4141 ggatcgtgga agtcgtggta cttctgttgt gtgtattggt cggcggagtg atcggctatt
     4201 ttgtctacga gggacagctc tgggagaacg ccagtagacg gagccatgaa caggttatgg
     4261 cactggagga agagaacaag acactgagcg agcagaacag taagctggaa gagcaggtga
     4321 cggaacaggc ggaagagata cagatcttaa gcgacaccgt aagccagaag gtacagagtg
     4381 agagcgaatt atcagcaact ctggaaaagc agagccttcc tacggaattc cctctgacgg
     4441 gttctgcatc catggaggaa gtaagtgagg gcgatcccat gtgcatattc catgcatcgg
     4501 tgggaactac ggttgtggcg gtggccagcg gtacggtcat tgccataaat gatgacgaga
     4561 tctacggaca cagcgtgtgg gtggatcatg gcaacggtta tgtcaccatc tatcggaata
     4621 aaggagatac cacagtgaat gtgggggatt ccgtagtaca gggaactacc ttgtttgtca
     4681 tcggcgaaga taacgcggat ctgggatacc agatgcagaa agacggaagc tatattaatc
     4741 ctatggatat gctggcaatc agtggttgat tgcagaaagg tttggaagat acatgttagg
     4801 aaaaaagaat gcagatacaa aaatgagcac actgatcggt gccggagcgg agttgaccgg
     4861 agattttaca ttggacggtt ccgcaagaat tgacggtaag attggtggta acgtgacggt
     4921 gacaggcaac ctgatcctgg gagccggaag catagtcaca ggagatgtac aggctgcatc
     4981 tgttctgatc ggcggggaag tgaccggaaa tatcactgca ccggagaaag cggaactgac
     5041 agcttcggcc aaagtgctgg gggacattgc cacaaaggtg atcgttattg atgaacatgc
     5101 cgtattccag ggaaaaatcg atatgaacca gacagttcct gacagaaaag gtaaagccag
     5161 aacggccaaa gcggtccggg caggacgcaa gagtgccaag gcagccatcg cagaagctct
     5221 gaaggaagtg gaagaggaag aaaagagaga aaatcagaag acttcacaga cagcagagac
     5281 ggtggagaag acagaaactc cagctgcgga aacaacagca gccaatacaa gcgaagcata
     5341 agaagaataa aagataaaat aaaaacagga gcgccgtttt tgagtgctcc tgtttttatt
     5401 cattaagtta agaattctgt tccgtttcct ttttctccat acgtccaaag accagctcgt
     5461 aaccgtcgtt gccgtaattc agagaacggt ttacgcggct gatggtggcg gtggaagctc
     5521 cggtcttctc ggcaatctcc agatatgtct tgccgtcctt cagcatggca gccacttcga
     5581 accgttggga cagagacaac agttcgttga cagtacacag atcttcgaag aaactgtagc
     5641 attcctcacg ggactgcaga cacaacaccg catcaaaaag tgaatctaca gcgtccgttt
     5701 tgattttttt attcattagc gacacacctt tccatagttg taatcaatac aacgcaacta
     5761 ttagtatttt agcatactaa agttgtaaag taaaggagtg aaacaaaata gttataaaat
     5821 cttcataaat tcagagaaca gagaaaatgt gcaactgttg acatgtagtt ttgaatacta
     5881 tataatgaat gcaaaagaaa acaagcgctc gcagagcgag catttgtttt cttgcatgca
     5941 ttaacgagag aaccgtctgc gaagcagaca gcagagtgcg aagcacgggt tctcgagtca
     6001 atatttttgg aaactacctc acggaaagga cagcaggaac attatggcag caacaacaga
     6061 agaattactt cagatgatct tacagagtct ggagaaagta gaatatatca agtcggaaga
     6121 tattccgaat attgatctgt atatggatca ggtactgacc tttatggatc gtaagctgcg
     6181 ttccgcagtg cggccccaga ccgaagaccg catcctgacc aagaccatga tcaacaatta
     6241 tgcgaagaat gatctgctgc cgccgccggt gaagaagaaa tattccaaag agcatgtgct
     6301 gcttcttatt tttatctact attataaagg gatcattccc atgaatgaga tccagacgct
     6361 gcttaagccc cttactgacc gctattttca gaacggacag gtctttgacc tggagagcgt
     6421 ctacaaggaa gtggtggtgt tggagaagga acagctggag gatctgaaaa aggatgttgt
     6481 ggagaaattc cgcagagcag aggattcctt taccgaagtg accggagaag agcaggagtt
     6541 tttacagaaa tttgccctga tctgcctgtt gagctttgat gtctatatga agaagatgat
     6601 tgtggaacag ctggtagcgg atctggcaga tacgatgaaa gcacaggaac cggagaagga
     6661 agctcataag aaaaagaatt agaccgtttt ggaaagtaaa aggacaatag aatgaaagca
     6721 atcatggatc ttcatacgca tactctggca gcagggcatg cgtatagtac cttgttggag
     6781 aatatagatg ctgcattggc aatgggctta cagtacctgg gaatgagtga gcacggaccg
     6841 acttccccgg gaggcccgca taatttcttt ttcagtaatt ataaagtgat tcccagaata
     6901 tatgacagag aagagaacac cggccgggta ataccgaagc cagatggaag tctgcgcctg
     6961 ctatgcggcg tagaagcaaa tatctgcagt acaaacggtg aactggatct ggaagagcgc
     7021 tatctgcaga aaatggatta tgcccttgcc agcattcatc cctttgcctt tactgcggga
     7081 agccgcaagg agaatacact ggcatctgtc agagcttttc agaatcctta tgtgaaaata
     7141 cttgggcatc ccgatgacgg cagatttccg ttggattatg acgaactggt gcgagaggcc
     7201 agacaggcac aggcggtgtt ggaagtgaac aattcatccc tgaatcccca gtcggccaga
     7261 caaggaggca gagaaaatat tacagagctg ttaaagactt gcatgaagta tgatcagcct
     7321 gttataatgg gaacggacag ccatatgtgc tttgccatcg gagcatttga tgatgcagag
     7381 cagctgatgc gggaactgga atttccgaag gaactggtgt tgaactatga tccggagaac
     7441 atccgtaaac tgatcaacat aacattatag ctgatcctgg ccggcaagac cggacaagaa
     7501 tttaagccgc agacggcagg ccccgtaaga gggcagatag gagaacaaat gaaagacgca
     7561 aattgtggat attgtatgag aggagaactg ttagacaagt tcggtatttt tatctgtgat
     7621 ctgaaggtaa gcagcctgat cctgttcaag gagcagagcc atcccggcag atgtatcgtg
     7681 gcatataagg accatgtcag cgagatcgtg gacatcagcg aggaagagag aagattattc
     7741 ttcgaggatg tggctcatgc agccaaagct attcatgcag cattccatcc cgataaatta
     7801 aactacggcg cttacggtga caccggctgt catctgcaca tgcatctgtg cccgaagtac
     7861 aaggacggtt tcgagtgggg cggaaccttt gagatgaacc ccggcaagac ccttctgact
     7921 gatgcggagt accaggagat gatcgacaag atcaaggcaa atctgtaatt acccggaacg
     7981 ttttccctga ggttacatag aataaatatg taatctcagg gaggatgaac gattatgaaa
     8041 agaaggaaag taacggtagc agtattgtct gtagtgcttg cagcggctgc acttacgggc
     8101 tgcggcagcg cacagcagac gaacggacca aagaagacca gagtggtgct caatgaggta
     8161 gcccactctg ttttttatgc accgatgtat gtggcaatcg aagaaggata ttttgaagat
     8221 gccgggatcg atctggagct ggtgacggga tttggcgcgg ataagaccat gacggcagta
     8281 ctgaccggtg aggcggacat cggctttatg ggcagcgaga gcaccattta cacctatgct
     8341 ggcggaacgg aagattatgt ggtgaatttt gcccagctga cccagcgggc tggcaatttc
     8401 ctagtggcga gagaacccat cgatgatttt tcgtgggata tgttaaaagg tactaccgtc
     8461 ttgggcggta gagcgggcgg tatgccggag atggtatttg aatatattct ggagaaaaat
     8521 aatatggatc cgacaacgga tctgtccatc gaccagagca ttgacttcgg ttccacggca
     8581 gcagcttttt ccggcggaca gggagatttt accattgagt tcgagcctca tgccacattg
     8641 ttggaacaaa agggtgacgg ttatgtggtg gcctctctgg gtgaagaatc cggttatgtt
     8701 ccttatacct cgttctcagc aaagaagaat tacctggaaa aaaatcccga gaccattcag
     8761 gcattcactg atgccttgca aaaaggcatg gattacgtac aggagcatag tgcggaggaa
     8821 attgccaagt gtattgcacc ccagtttgcc gagaccgaaa gtgacacatt gacagctatt
     8881 gttgccagat atcaggaaca ggatacctgg aaagaggatc tgatcttcca taaagatgcc
     8941 tttgacctgt tgcagaatat tctggaggat tcggatgtac tgaaagagag agtaccttac
     9001 gaggcactgg taactacgga attcgcagag aaagcaacgg caagataagc agaataaaat
     9061 aggataaaaa gggggaggat cagacgtcct cctccttgat gtgtatattt ttgatggccc
     9121 ataatttcaa agctttggca ttggtactga tcttctccag ggaatccaga atatcctgga
     9181 aggccgggcg ctcatcttcg gagatgatgc cgtcctcgga gatggcaatc agggaagccc
     9241 gcagggtgtc gatatctttt aaggaaccca gtaccttcaa tgccagccgg tcaaagtcca
     9301 caatgtcgac ttccgataca ctgctgcggc ccagaggaca ttccctggca cagaaggaat
     9361 tgcaaagctc cggtgtattg taggccttgg ccatggcctt gacttcctcg ggatagggaa
     9421 caatggtgtc cagttcgatc ctggccagtc tggtacggtc gatgtccacc agttcggcgg
     9481 ctttttcccg gctgctgaag tccgcattcg tctcagcggc ttcgcaacgt gcgagatagt
     9541 acatattatc agcggctttt gtagcttttt tacccatttt tctttcaacc cttttatatt
     9601 atagtttaat caaacatctc tagtatcaca gtttagtaga aaaacggcac attgtaaagg
     9661 aaaaatgacg atattttacg aaaagcagtt gagggaatgt gtaaaatttg ctaaaatata
     9721 agagatgtga aaaagaacag atttttcata taaaaacgga ggtgcggtaa tgggtttaat
     9781 cagagcagcg aaagatgcag tcagcagcat gatggctgac cagtggagag agtatttcta
     9841 ttgtgattcc ctttccaatg atgtgctggt agtaaaagga cagcagcgtg taaccaatgg
     9901 acgtaacagt aacaccaagg gtgtggagaa cattatctcc aacggctcta tcgtggcagt
     9961 caacgaagga cagtgtatga tcatcgtgga tcagggcgga attgtagagt tctgtgccga
    10021 tgcaggtgaa ttcgtatatg attcttccac cgagcccagc ctgttctacg gaaatcttgg
    10081 tgagagcgtt aagaatacat tttccaatat cgggaagcgt tttaccttcg gaggtaatgc
    10141 ggcaaaggat cagagaatct acttttttaa tattaaggag atcatgaaca atctctttgg
    10201 tactgcaagc cccattcctt tccgtgtgat cgatcagaat atcggactgg atctggatac
    10261 ttctttacgt tgtaacggtg aatattcctt ccatctggta gatcctctgt tattctacaa
    10321 gaatgtatgt ggtaacgtaa cagagtccta caccagaagt gaactggtag cgaagatgaa
    10381 ggcagagctg ttgactgcat tgcagcccgc actggccaag atctccgcaa aaggcgtccg
    10441 tcccagtgag attcccgcac acactgagga actgacggag gctttaaaag atgaactttc
    10501 cgatctgtgg accaagcttc gcggtatcga gatcgttact atcaatatca actctgcaaa
    10561 gattcccgat gaggatgcga agactattgc tgatctgcag agaaccgcag tactgcgcaa
    10621 taccggaatg gctgcagcta ccatgactgc tgcacaggcc gaagctatga agatggcagc
    10681 atctaatact tctacaggac ctatgatggc atttgccggt atgaacatgg cgaatatggt
    10741 aggtggtatt aatgccggcc aactgtacca gatggatgca cagcagaatg gtatgggtat
    10801 gcagcaggca caggcagcac ctgtgcaggg gcaggctcct aagatgcagg caccggtact
    10861 aggctggacc tgtagctgcg gtgctgcgga taaccggggc aaattctgtt ccgagtgtgg
    10921 cgcacccaag ccctccgatg caggttggac ctgcagctgt ggtgcagtaa accagggcaa
    10981 gttctgttcc gaatgcggag caaagaagcc ggacggcgca ccgatctata aatgcgacaa
    11041 gtgtggctgg gagccggaag atcctgcaca tcctcccaag ttctgcccgg agtgcggtga
    11101 tccttttgac gagaacgaca gagtataata catactcagc agaatatttc tacaaaacag
    11161 cagtacagcg gatgccttta ggcgtctgct gtactttgct gtaacagtgc ggctttgcga
    11221 atggcgcgct gaactgttaa cagttcagat atgtggtgca actttggcac ctgcgttcct
    11281 gaacttatgc tatacaaaca ttacaacaga atgaaaaaca gagagaaaca taaatgagcg
    11341 gaaatctgat tgcaattatc gtcatattgg tattacttct ggtgcttgca ggaatcattt
    11401 attatgccta ttgcaacatc aggaaaaaac tgcgggatac ctcccggatg ttattcggaa
    11461 cggactccat gatcgaggga atgaaacaga gagaaaagga agtggaaatg acaccgaagt
    11521 cggtctcttc cgcaaccaac ctgtacatgc cgtccattat gcgggatttc ccggaatttc
    11581 attatgatga gatgaagagc agagcggaga atgtgctgac ctcctatctg cagagtatta
    11641 cgaaacagaa tccggctctg ttgtcggagg gaaccagaga attgaaagaa cagctcagac
    11701 tgcggctgga aatgctacag aaccagtccc agaaggagag ctttgaaaat atccatattc
    11761 acagaacgga gatccaccag taccggaagc agaggggaag acagagtatt gtcctgcaga
    11821 cggcagtgga gtattttcat gcattaaaag agaacggaaa agtaataaga ggcagtgaag
    11881 agcataaaga acaggccaag tataacgtgg aactggtcta tatccaggat caggatatga
    11941 tcgagaatca ggaggatgcc ggtctgggac tgaactgtcc gaactgtgga gcaccgcttc
    12001 cgggactggg agccaagaaa tgtatttact gtgacacgcc gatcgtggaa tacaatctcc
    12061 gtgtctggaa tttcagccgg gtggaagagg tatagagaaa gcaaaagagg gtctgcatgg
    12121 aagaaaaatg tatgggattg aaaaaagagg ttgcattgca ggaaaaacag acaggggatg
    12181 tggacagaca gctggatgag accaccagag aactgcaccg ggagtgggaa aagctgatgg
    12241 ctgtgatccg tatgtccgga gatctgattt tcgaatatga tattgtaaat gacaagatgt
    12301 attatgccgg tccgggagag ggaattctgt attcctggca gatcacggag gggtatacag
    12361 atcagctgct caaagaagcc gggaaccgga cagaaacggt agaacaacag ctggcagaag
    12421 cactgcgcag tggcaagccg gatatttata tcgaactctg caagaccgat gcgcagggga
    12481 agccccggtg ggtgaaggtc atcggacaga ctttttatga tgaaaaggga gaaccggagc
    12541 gggttctggg aaaggtctgt gacattgatg accagaagaa aaaagagtgg gaactgcggg
    12601 aaaaatctca gaaagattcc ctgaccggat tgctgaacaa ttctaccatc aaacaacaga
    12661 tcggtgccag gctacagagc ctgaaacgcg ggcagactgc ggatctggtc gtacaggatg
    12721 tggacagctt taagaagatc aacgacacca acggccatct gtttggggat gcggtattgt
    12781 gttcctttgc ggatgaactc agtaatattt tccccgaggc attaaaaggc cggatcggtg
    12841 gggatgaatt tgtcttctat gtggaggaca tggacagaga acagctggag gagaaattaa
    12901 aacagctgaa ccgctgcatg tctgaccgtt atgatgatga caagacaggg ctgcacattt
    12961 cctgtagcct gggcgcggcg gtgacagacg gcacggtgac ggattacaat ctgttgttcc
    13021 agtgggcaga tgcagccctg tatcaggtaa agagcaaagg aaagggtgct tattacctgc
    13081 tggatgtcaa ggaaggtgtg gcattgcccg gcaagagtta tctggacagt gaagagaaca
    13141 aggaggacga gattgcccat aaagaggcac agtttgagag tgcggaagat ttggtattat
    13201 tttgcatgga tctgttggaa aatgtgccga atctgaccag tgcactaaag gtgatctgtg
    13261 agcggacctg tagctttttt ggaattgatg acatgatctg tatggaagtc aacgaggctg
    13321 gaccaacaat ccgttatcaa tggaatcgtg aagagaagaa agattttgcc agaagaatcc
    13381 tgaatcccgg cgtgttcgaa tgggacagga tccggaaaaa ggccgatgaa tccggtgctg
    13441 tgatctaccg gcagtcagag actccggatg tctacaagga agaggcggag tccgtgatga
    13501 ttgtaatgtc cacggccgct aagggattaa aggccagcgt tattttctcg aatcgccaca
    13561 cagacagaag ctggcaggag gagaaaaatg cactgtgcag aatttctcac cagattttta
    13621 accgactgcg cacgctgaag aatgcggaga aggatcagcg tgagctgaac cggaaactga
    13681 attatgatgc tctgaccgga ctgccgatat ataacaagtt tgtggataag ctggaggcct
    13741 atatggctgc ccacgggaaa tcaggattgt tttttgtgtc atctgatttt tcaaatttcc
    13801 agtatgtgaa cgaaatgtac ggttatgaag tgggagatag aatcctgcat gattttgcga
    13861 tagccttgca ggaaaagtgc cagaacggcg tactgttctg ccgtgtaact tcggatcatt
    13921 tcgtgggagt gctgaaggaa gatacggtgg agaaggccag agacaaatat ctggaattca
    13981 ccaatgcttt tgtgcagaag atcaacagcc gctatgacca gtgtaatctg gtcattgcca
    14041 cgggaatgta tgaaatcctg gagaatgact ggtcggtgtc ctccatgatg gataacgcca
    14101 atgaggccag aaaacagtgc aagacccaga aggtagacag tgcggtggaa atctatacag
    14161 agaagatccg taagaatctg gagaatacca gagagatcgt atctggtatg gtagctgcat
    14221 acaataataa ggaatttcgg gcgtatctgc agccgaaagt ttctttgcac accggaaaag
    14281 tagtgggtgc tgaagcactg gtcagatgga tccgggctga cggttctatg gtaatgccgg
    14341 ggagatttat tgatgtcttt gagagaaacg gatttgtgac caaggtggat tttgccattc
    14401 tggatcaggt actggaatat ttgcaggatg ctttggcgca gagagaagaa gtggtgccga
    14461 tctcggtgaa cttttccaga aggcataatg aatatacaga cttcgtaccg aatattttaa
    14521 aacgcctgaa agactacaaa gtacccagtg aacttttgga agtggaagtg acggaatctg
    14581 ttttcatgtc ggatctaaat aaactgacca acaatctgga gactctccgg gataaagggg
    14641 tagagatttc agtagatgat ttcggcagcg gttattcatc cctgaatctg ttgtccaagg
    14701 tagcggtgga tacggtgaag ctggacaagc agttcctgga tgacaccatg aacagcgaac
    14761 gggaagaaac ggcattgaca attatcaaat atctgactaa aatgttgaag catctcggct
    14821 tcaaggtatt ggcagagggg gtggagacga aggaacagtt agacatgctg cgtctggcgg
    14881 actgtgacat cgtacagggc tatttttatg caaagcctat gccaatcgat gatttccgaa
    14941 agtttttgca ggaatttaat gaaagggata atgcgtaatt catgagtatt tatgatacct
    15001 taaataaaga acaaaaagaa gcggtgcttc atacggaagg accgctcctt ctgttggcgg
    15061 gcgcaggaag cggcaagacc cgtgtcctga cccacagagt ggcttatctg atcgatgaga
    15121 tgggagtaaa tccatggaac attctggcca tcacttttac caacaaagca gcccaggaga
    15181 tgcgggagcg tgtggatcag atcgcaggtt tcggtgcgga tcaggtatgg gtagcgacat
    15241 tccatgccac ttgcatgagg atcctcagaa gacatattga ccggttgggc tatgatacga
    15301 attttaccat ttatgatacg gatgaccaga aatctgtgat aaagcaggtc tgcaagcgcc
    15361 tgaacatcga cacgaagatg tacaaagagc ggacgctgtt atcggaaatt tcctccgcca
    15421 aggacgagct ggtggatgtg cgggaatttg aagtaaaatc cgtgggagat tacaagaaat
    15481 ccgtgatcgc caaggtctac agagagtatc aggagacctt gaaaaagagt aatgcactgg
    15541 attttgatga tattattgta aagactgtgg aactgttcaa aagctgcccg gaagtacttt
    15601 ataattatca ggaacgtttc aaatatatca tggtggatga gtaccaggat accaatacag
    15661 cgcagttcga actgatccgt ctgttggcgg acggttatcg caatctgtgt gtggtaggtg
    15721 atgatgacca gtccatctac aaattccgcg gtgccaatat ccgtaatatt ctggactacg
    15781 agaaggtata tcccgatgca aaggtgatta agctggaaca gaactaccga tccacccaga
    15841 acatcctgga tgcggccaac gcggtgatcc gtaacaacag aggccgtaag gaaaaagccc
    15901 tctggacgga aaaaggatca ggcagccggg tacatttcag gcagtttgac aatgcctatg
    15961 aagaggcgga atacattgcg gacgatattg ctgacaaggt gaaaaatgac ggcattgcct
    16021 atgcggactg tgcggtgttg taccgtacca atgcccagtc gcgacttctg gaagaacgta
    16081 tggtagtgga gggcattcct tatcatgtgg taggtggagt gaacttctat gccagacagg
    16141 agatccgcga tattctggca tatctgaaaa ctattgataa cggcagggat gaagtggctc
    16201 tgcgcagaat tattaatgtg ccgaaaagaa gcatcggtgc ggcatccttg gaaaaggtag
    16261 cagactatgc acagatgaag gatattactt tatttgatgc attgtgtgaa gcagatcaga
    16321 tcaagggact gggaagagcg gagaccaaga tccgtggatt tgtgaatctg atcgaggtac
    16381 ttcgcagcgg attgtcttcg tatacattgc cggatctgat caaatctctg ctggaacgga
    16441 tcgattatgc agaatatctg cgggatcagg atgaggaatc cgctgaggat cgtctgggga
    16501 acgtagatga attgatcacc aaggcggcta catatgagga gactcacgac gagccttctc
    16561 tgtcggaatt tctggaagaa atcatgctgg tggcagacat tgacaatgtg gagaacggtg
    16621 acaaccgggt gctgctcatg acgctgcaca gtgccaaagg cctggaattc ccggtggtgt
    16681 atctcgccgg catggaagat ggattattcc ccagctttat gaccattgct tccgatgacc
    16741 cgttggagat cgaggaagag agaagactgg cttatgtggg aatcaccaga gccaaggagg
    16801 atctgacgct gacctgtgca aagagcagaa tgctgcgtgg ggagactcag tacaatccgg
    16861 tgagccgttt tgtgagggag attccgaagg aattattgga taataccctg ccgcccagca
    16921 gaagataccg ggacgatgat ctggaagatt ttcaggcgag aagagccaat gaagcagctc
    16981 ttcgtgccat gggactggag gcgattgcat ctccccggtc tggatccggc tcggatagtg
    17041 gttttggcgg cttcggtaat accggctttg atccaagacc caaggcgacc ttaaaacccc
    17101 gtgttacggc aaaggcggat aaaccttata tttccaaggg catcagcgga ctgaaccagc
    17161 tggccggact gcagaagggc acggagttca aggcaccgga gaaactggac tacgaggtag
    17221 gtgacagagt gcgccatatc aaatacggcg agggcacggt actaaatatc gtgagagaac
    17281 ccagagacta taaggtgacc gtggaattcg accaggcagg gcagaagatt atgtatgcgt
    17341 cttttgcaaa actaaagcag ttgtaagaaa ttataagaat ataagaataa gaaatgtatt
    17401 tcctcaaata tggtttttct atagtatttt tgatgaaaat atggtatcat ataaaggaat
    17461 gaaaacaaaa tgctttgcat ccataaatac gaatagatgc aggaaaggca ggtaaataat
    17521 atgaacagaa ttgaaaatct ggtagatctt gacaaattaa atgagctgaa agtcagcgaa
    17581 ctgaagaagc ttaacaagct gttgggcaag gaagtagaag agaagaagga acgcaatgtc
    17641 ctggtatggg tactggcgat cattggcgcc gtagcagctg ttgcagcagt ggcttatgct
    17701 gtataccgtt tcttctttgc acaggattat ctggaagact tcgaggatga cttcgaagat
    17761 gaagcagatg acgtggaaga ggatgatgac gacttcttcg aggatgaagg agagaactaa
    17821 cccatgaaaa aaggacaggt atatgaagga tctgttgtaa gggtcgattt tcccaacaaa
    17881 gggatcgtct gtgtgggaga tgagactgct gtagtcaaga acagcctgcc cggacagaaa
    17941 gtcaagttct cggtgaataa agtgcgcaaa gggaaggcgg aaggaaggct tttggaggtg
    18001 acggaaaagt cacctctgga aaccggccgg acgtgttccc tttttgggtt gtgcggcgga
    18061 tgtacctatc tgtctcttcc ttacgaagaa cagctgaaag tcaaggagga acaggtaaaa
    18121 cgcctgctgg acagtgtgtt aaacaagcag gaggaagcct gggcttttga gggcatcaag
    18181 ggcagcccga aggcttatga ataccgtaat aagatggaat tttccttcgg agacgagtac
    18241 aaggacggac cgctggcgct gggcatgcat aagagaggca gcttctacga tatagtgacc
    18301 gtagcggact gtgagatcgt ggatgcggat taccgcctga tcttacagac agtgagagat
    18361 tactttgccc gggcgaaggt aagctttttc cacagaatga gccatgaggg gtacttacgt
    18421 cacctgctgg tacgtaaggc atcccgcacc ggagagattc tggtggcact ggtgacgacc
    18481 tcccaggatc cctggcaggg agagacggcg gtggaaggaa gcttggatgt ggatgcgctg
    18541 atcacaggat ttaaggatct gctgctttcc ctggaacagg ataggaagct agccggaaaa
    18601 ttcgcaggaa tcttacatat taccaatgat tccattgcag atgtggtgca gagtgatcgt
    18661 acggagctgt tatacggaca ggagtatttt tatgaggaac tgctggggtt gaaattcaag
    18721 atctccacat tctccttttt ccagaccaac tcttacagtg cggaggtact gtaccagacg
    18781 gcaagagatt atgtgggcaa cctgggcggc agtgacaaga ctgtgttcga cctctacagc
    18841 ggtaccggta ccattgcaca gctcatggca ccggcggccg gaaaggttat cggcgtggaa
    18901 atcgtggagg aagccgtaga ggcagcaaag aaaaatgccg ctgccaacgg actggataac
    18961 tgcgaattca tcgccggaga tgtactgaag gttctggacg aagtggaaga gaagccggat
    19021 atgatcatcc tggatccccc cagagacggc atccacccca aggcactgcc gaagatcatt
    19081 gcctacggtg tggagcatat tgtttatatc tcctgcaagc ccaccagcct cgtgagagat
    19141 ctggaagtat tcttagagaa tggctaccgc gtggacaagg ccgtggccgt ggaccagttc
    19201 ccctggaccg caaatgtcga gacggttgtc ttactttcca agggcgctaa aggtccggtg
    19261 gacctttgct caacgcggac cgaagtggaa cggagctgat caaggttatg accggtatcc
    19321 gccgctgcgg aaagtcggtc atgctggagc tgattcatcc gcaggaatta caggataagc
    19381 attatttccg taagcatggt gcagatgcat attacggaca gaattaacag gatatcgaga
    19441 gggtataaaa cagatgaaga tgaaaacagc acctatgggg tggaacagtt gggattgtta
    19501 tggagcggct gttacagaag atatggtacg acggaacgct gaatttatgg ctgatcatct
    19561 gaaggagtac ggttgggaat atattgtggt ggatatccaa tggtatgagc cttatgctgc
    19621 aaccaatgag tatcatcctt ttgcggatgt agtaatggat gagtatggaa gactgcttcc
    19681 cgcagtaaat cgttttcctt ccgcagcaaa cggtgcgggc tttggaccat tggcagaata
    19741 tgtccattct cttggattga agttcggcat tcatattatg agagggattc cacgacaggc
    19801 tgtacatcag aacacaaaaa tcatgaacag tgacagacat gcccgggaaa ttgccaaaac
    19861 aaacagtatc tgtgcctgga atacggatat gtatggtgta gatcctgaga aagacggggc
    19921 acgggaatac tataacagta tttttgaatt atatgcttcc tggggtgtgg attttattaa
    19981 atgtgatgat attgcacggg aactgccaca tgaggaatcc gaactgatta tgctgagcaa
    20041 agcattgcat ggttgcggaa gacccatggt tttaagtctg tcgccgggac ctgctctttt
    20101 agaaaaggca gagctttata agcagatttc caatatgtgg agaatcaccg atgatttctg
    20161 ggataagtgg gaactgttat atgatatgtt ttcaagagca gaaaaatggt gcacccatgc
    20221 aggtgcaggg cactggccgg atgccgatat gcttccggtc ggaccgatac ggcaggtata
    20281 tgatgtgaat aactggacga atttcacaca ggatgagcag atcaccatgc tgacactctg
    20341 gagtatcatg cgttctcctt tgatgctggg aggagagctg acagggtttg acgaattcac
    20401 tatgaatctg gtaaccaatt ctgaaatact tgccatgcat gcaaatgcac ggcattcgca
    20461 tcaggtgtgg agacgtgaaa tcgatggtat agagcatgca ttgtggattg cagcagatac
    20521 aaagggtgga tattatgtcg cggtatttaa tctgggtgat aaagacagtg acatatccat
    20581 accgcttgct gatctggaga tttatgatgg agtaaatgga acggagcttt ggagcggaga
    20641 gcacgtggaa gagccaaaga gccttagtgt gtcattaaag agtcatggtg ctagggcata
    20701 tcattttacg tacaactaaa aagcatacta ctgttaatgt atagagatct tcaatcatga
    20761 tctcagtttt gagattaacc ttatagtcat tttcagcacg ttggcgatct ttttcttctt
    20821 gccggttctg actcatcatg ataagaggtg cctgaatcgc agctacacag gacagcacga
    20881 gattaagcag gataaaaggg aatggatcaa aggctttgac ggcaagcaga ttcagaacct
    20941 cctcattggt catatcatgg cgaatttctt taagcacttc ttttaatagt ttgcggtttc
    21001 ccttcatgct ggtctccttc tgtttattcc cggtaaatct gtgcaaaccg gaaatttctg
    21061 atgaataacg tggtgcagga tcgttttcga ttattatatt ttactgtgag attgtgatat
    21121 tagcaatagt gaaatggggc agagaagagt tgaaaaggca gacggataan nnnnnnnnnn
    21181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt gatattagca atagtgaaat ggggcagaga
    21301 agagttgaaa aggcagacgg ataagaaaca aagataaaat agtaaaaata gaatagaaca
    21361 aagaaagaat atggagataa aacagaaagg aataaacaat gaatgtaaat cctataacaa
    21421 gattggatta tccagatccg gacgtgatcc gcgtagaaga cacctattat atggtatcca
    21481 caaccatgca ctttatgccg ggctgtgaga ttctgcagtc ttttgatctg gtgcactggg
    21541 agcatgtgac ttatgtctat gagacgttgg atcatacgga tgcgcagcaa ctgaaaagcg
    21601 gtcagaatat ttacggacag ggcatgtggg cagcttccct gcgctatcat gaaggccgat
    21661 tttgtatctg ctttgtggca aatgataccc gtaagactta tttgtatact tcggaaaaaa
    21721 tagatggtcc atggaaaaag cagctgatag aaggctttta tcatgacggc tctttattat
    21781 ttgatgagga tggcagaaac tatattgtat atggaaatga tgaaatctgg attacagaat
    21841 tgaatgaaga tttgactgca ccaaagaagg acggattaca tcgtctttta gtcagtgatc
    21901 ataagaaccc ggagctaggc tatgagggtt ctcatattca aaaaataaac ggctattatt
    21961 atatttttct gattcattcc ctgagagacc gctggatgcg gacggaagca tgcttttaca
    22021 gtgattctct ggaaggagaa ttcacgggtg gagatgtact ttgtgatgat cgtggatatt
    22081 gtggacaggg cgtggcacag ggaggaatag tggatacgcc ggatggaaag tggtatgcaa
    22141 tgctgtttca ggattccgga gcagttgggc ggattcctat tctggttccg gttacctgga
    22201 aggatcattt tccgtttttt ggacagaacg gacatgtgcc ggaagaaata gaggttcaca
    22261 gtaccagacc aggatatatt tatcagccat tagttggaag tgatgatttc tgtaatgggc
    22321 tgcttccgca ctggcagttt aatcatgatc cagatccacg gtactatcat ctggatgcat
    22381 tgcagggaac ctatacaatt acaacacggg aagtttgtac agagcttaca caggcagtaa
    22441 atacgcttac gcaaaggatg agttatccgt cctgcgcagc tgaggttact gttgatgctt
    22501 ctgcattgaa agagggcgat gtcgcaggtt tgtgtgcgtt gcagagctgt tatgctgcag
    22561 ttggcattac aaaacatcat ggaaaatatg aagtccttat gctggataaa aaagaagatg
    22621 gtatattgga tatgacggaa ggaactgttg ttgaatcaca ccagatggat tttcgtcttg
    22681 aagcagattt ctgtaacatg aaggatgaag tacgctgcta ttaccgggta aataagggag
    22741 actggctacc catcggaacc gtgcataagc tgtacttcaa actggatcat tttactggtt
    22801 gtcggtttgg tctgttctgc tatgcggcag aggaaacagg aggaagtgcc tgcttccggg
    22861 attttaagta ttatgatgtt gatgaaatct cataaagata gaactgcata aaataagtgt
    22921 cggaatttac tcaaattttt cctactttgg acattggttt ttacaacggt attgtatagt
    22981 gaagcagagg aaaaacaaaa catgtttcag agaggagaaa cgattatgag aattaagagt
    23041 atgatgaagg gtatgcttgc tgttgtaatg gcaatgtcta tggcagcatg ctccggagca
    23101 cctgcagcag accagacttc ttctgagtca ccgtcaccgg cacagacaga aagtacagca
    23161 gcaacagagc aggaaacaat cgtaacagaa gtatctgcag aaacgcagac gcaagaagaa
    23221 acagcaaaag caccgaaata tgttttctgt ttattggtga tggtatgagc
//
LOCUS       DAWDHD010000024        22582 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_167_length_22582_cov_10.029431, whole genome shotgun sequence.
ACCESSION   DAWDHD010000024 DAWDHD010000000
VERSION     DAWDHD010000024.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 22582)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 22582)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..22582
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_167_length_22582_cov_10.029431"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         6347 a         4701 c         6287 g         5247 t
ORIGIN      
        1 aggatatgat gaagatgtac tccatgcccg gcatggatat gagtgctttc ggcggtgagg
       61 gcgagaccct gatcctgaat gccaaccatc ctctggtaca gtacatcacc gagcaccagg
      121 acggcgagaa cgcagaaatg atctgcgagc agctgtacga tctggcaaag ctccagcatg
      181 cacccctgtc cgccgatgcc atgaccaagt tcgtggcaag aagcaatgac attatgatgc
      241 tgttgaccaa ataaaaatga cggattaaaa acaaagggaa tcacaatctg ggaacggttt
      301 gtggttcctt tttgggctac acagcggttc ggggacagat gcagaccggt accggaatta
      361 gtatttagtg aatgcatatc acccgtctgt taatcaagta gatgtatgat gaagacaaaa
      421 cggatagaag ttagtctact tagtatatgg gaacatgcac atatttttct gactttgcat
      481 attctgtaag gaacatttgt tgcctgaccg gatcaagaca gacaaaacgg cataggcaga
      541 aaggacctct gtccatggac actacttata caggaacact tcagatcagc gttgtctccg
      601 ctctggggat gacaccgata cccggcgcga ccgtcactgt ctcctatacc ggggatccgg
      661 attccccgct ggagacgatg actactgacg aatccgggca aacccctacg ataacactgg
      721 atgcgccgcc gagagagctt tctttatctc cggatattac ggcacagccc tattcggagt
      781 acaacattca ggtgactgca gaaggctttg aaccggtact ggtatctggt tccgaaattc
      841 tggcaggcga attttcccta caaccgatcc gcatgaatcc gctgaatgta acagaggagg
      901 aagaaaaagt agtagtaata cctgcacata cgcttttcgg agagtatcct ccgaagattc
      961 cggaagaaga aatcaaacct atgaacgaga ccggggagat cgtactgagc cgtgtggtga
     1021 ttccggaata tgtgatcgta catgacggtg tcccggagga tcccactgcc cggaattact
     1081 gggtgcgcta taaggattac attaaaaatg tggcctccag cgagatttat tccacctggc
     1141 cggagagtgc aatttatgct aatattctgg tcattcaatc atttacgtta aaccgtatat
     1201 atacggagtg gtacagggga cgtggatacg attttacgat aacttcttct acggcgtatg
     1261 atcaaaaatg gatctatggc agaaatgtgt tcgaagagat cgactatctg gtagattcca
     1321 tttttaccaa ctatttgtca aggcccggtg tacgacagcc cattttcaca tcgtattgtg
     1381 acggtaatcg gactacctgc cgggggctga gccagtgggg ctccaagtca ttggcggatc
     1441 agggatattc cgctattgat atcattcatt attattacgg caatgatatg tatatcaatt
     1501 ccgcagatat tatttcggga gtgccttctt cctggccggg gtatgatctg actatcggtt
     1561 cttccgggga aaaagtccgt cagctgcaac agcagttgaa ccggatcgcc agaaattatc
     1621 cggcgattcc tacgttgaca cccgacggaa tctatggctc cggtactgcg gaggctgtcc
     1681 ggacgttcca gaagatattt aatcttcctc agaccggaat cgtggattat cctacatggt
     1741 ttgaaatctc ggatatttat gtgggggtga cacgtatttc ggagccggat gcgtagtaaa
     1801 tgggaagtta cgcgagcaat gagccgcgta acttcccatt taccatgttg taacaagtgt
     1861 aattccaaca attttcccgg acaagcataa aaaccgcttg acagataaaa ataaataacg
     1921 tattattgta ttaaataaat aatacagtaa aacaacaaga caatgaggga tccggggagg
     1981 catcgataga aacgatgcgt gacaggtaat aagaaacaga aaataagaaa caagaaagga
     2041 tgataggaat ggcgtgggaa ttggatgctg acagacccat atatgcgcag ctggtggagc
     2101 ggatccagaa agagattgtg tccggcaaat atcagccggg agagaagctg ccgagtgtca
     2161 gagatcttgc ggccgtggcg gcggtgaacc cgaatacgat gcagaaagct tttgcggaac
     2221 tggagcggag cggcctgatc gtgacacagc gcaccagcgg cagaaccgtg acggaggaca
     2281 ctgagctgat cgggaatacc agagaacagc tggcagccgg gcatgtccgg aattttatca
     2341 acaacatgca ggaactgggg tatcagagag aggagatccg gaatctcttc gataaggtac
     2401 aggcctgaca ggggtgcaaa tttatgatga aaataaagag aggcggaaat agaaatgaat
     2461 aatctggtag aaattgtaaa tctgaccaag aaatatggtg ccaatgtggc tgtgaacaat
     2521 ctgaccgtaa gtctccccaa ggggaagatc attggcctgt tgggacccaa cggcagcggt
     2581 aagacgacac tgatcaaaat gctgaacggt ctgctgacac cgacggaagg cactattatg
     2641 atcgacggaa attatgtagg accggcgacc aagagcaagg tagcttatct gccggaccgc
     2701 acgtatctga ccatgaatca gacgatccgg gagattctgg atttctttca ggacttttat
     2761 acagactttt ccagggaacg tgcggaagag atgttaaaga gtctggggat cgatcctgca
     2821 gtgaaaatgc ggacattgtc caaaggtacg aaggagaagg tacagttgat cttagtgatg
     2881 agcaggaatg ccagcctgta tattttggat gagccgattg ccggtgtgga tccggcagcg
     2941 agagattaca tcctgcgcac cattatcaac aactacaatc cggaagcaac ggtactgatc
     3001 tctacccatc tgatcgagga tgtggaacag gtactggatg aagtcatctt catgcgttat
     3061 ggtcagctgg tgttatatac atccgttgac aatatcagag aagagaaagg taagtctgta
     3121 gatgcttact tcagggaggt gttcgcatgt taggaaagtt aatgaaatat gaatggaaaa
     3181 atatctggaa agccggtaca ctgatgcttc tgggaatgct tgtggtcacc gtgattggct
     3241 gcgtggtatt acgcatgccg ggaggagtgg taacaggact tctggacaac aatgatataa
     3301 atgcgacaca gagctggttt gtactctcat cctttgtggc aacactgatc ctgtatgtga
     3361 tcatgctgtt ggcatccacc tggggtatgc tgatattcct gggcatccgt ttctaccgca
     3421 gtatgtatac cgatgaggga tatttgtccc atactctgcc ggtgacagcc aaccagctgt
     3481 tcttaagcaa gatactggtc tccggtgtct ggtatctgtt catttcgata ggcattggta
     3541 tttcagtggt ggcactggtc gtctctttga tgacaggact tttaaatatt ggtgaactga
     3601 gtagtgtgct gacacaatat aatggcaata tctgggaatt tctggcagat gctttttatg
     3661 aacttggcag aacatacgaa gaagagatgg gaatcaatct gctgcattat gggattacac
     3721 tactcttaac ctatgtggca ggacctttca ttactatggt cactctgttc ggagccctga
     3781 cgatcggtca gctgtcttcc aaacacaaag ggttcatggg aatcctggca tatgccggcc
     3841 tgacgatcct gtcttccatc atcggcagca ccgtacagag tgccttcatg tttggcacaa
     3901 atgtcatgag cagctccggc ggattttcgg taacgaccaa tatggcctat gacattaacg
     3961 tgatcaccag cctgctcatt gcagccatca tgtacggcgt atcttattac atcatgaacc
     4021 gaaaattaaa cctggactaa ccgggtctac accttgccct caactggtaa atttggtata
     4081 ataaatccaa atgacaacag ggaacgggtg aaggtgcatg caggagatta ttgatagaat
     4141 tttaggagga aacttcgatt atgagaacgg ttctttggaa ttctcctgcg ctaaaataga
     4201 gatttccctg tcacagggga caatatatga gggctctttt cacattcttt ctgcgtcgga
     4261 aggctatgta aagggatccg tgatctcgga ccatcttcgg atggaatgta tgacggatca
     4321 gtttaccggc tccgatgcag aaatttttta ctgcttccac ggagaagatc tggaggaagg
     4381 ggatgtgatc aagggctctt tttccgtagt cagcaacaga ggagaataca atcttccttt
     4441 tgtggtgacg gtggagcatg ggatgttgaa ttcttccatc ggggctatcc ggaatctgtt
     4501 ccattttgcc aacctggcga agagcaactg gaaggaagct gtgcggctgt tctatgatcc
     4561 ggaatttctg cggctgttcc aggggaacga tgcccatttc tataacagtt accggatcct
     4621 gtccacttat gagggcaacg aacaaaatgt agaggaattc ctgatctgta tcaataaaaa
     4681 gcaacagctg gaattcctga cggaagagaa agaactggtg aaaaaactgc ctcgcagcgc
     4741 agataattac ggcgtcacgg agaacaatct gaccatcgtg aggaacggct ggggatatac
     4801 gaatctccag atcgagtgtg agggtgaatt tgtctttacg gaaaaggaaa acattacaga
     4861 cgatgatttt ctgggaaacc gttgccgcct gccggtctac atcgacagca gcctgtgcag
     4921 acctggcaaa aattttggta aagtctacat atataatgct tatacttcac tggagatccc
     4981 ggtcatggta cagctgggag acggtgtagt ggcccggcat gcggatcaca gtcatatgca
     5041 gtgcatcacc cagatcatga aatattatga agaatcgaga ctgaaaaaga tcggtacggg
     5101 aacctggctc gcggagaccg gcaaactggt ggagcgcatg gtgaccatgg atgagaagga
     5161 tgtgccggcg agactgttcc aggcacagct tctgatcaca gaggaacgct acaatgaagc
     5221 cggatggatc ctggatcacg cagcggacat gttggaagcc caggtagcta ccggcggaga
     5281 acagtgggct tactatctgt atctgaccac actgatccac agggaccccc agtatacact
     5341 gcaaatggcg gagcaggtgg aacagatcta ccgctatgac aggacccgct ggcgtgtggc
     5401 atggctgctg ttgtatctgt cggaggaata caatcgttca acctccggga aatggatgtt
     5461 cttagaaaaa cagtaccagt acggctgcac cagtccggtg atctacctgg aagcactggc
     5521 ccttttgaac ggcaatccgg cgctgctacg taaattgaac agttttgaac tgcaggtgtt
     5581 gaacttcggt gtccgtcagg atgcggtaaa cgacagcctg atcgaacagc tgttgtatct
     5641 gtcgggaaga gtcagagagt attctccact actgggcaga attttacgaa gactgtatga
     5701 gaaaaagaag gatgtgcgga tcctgcagga ggtgtgcagc cttctgatca agggaagtaa
     5761 gacgggaccg gatgccttca cgtggtatca gatgggtgtg gaaagccatc tgaggatcac
     5821 gaatctctat gaatattata tggcgtcggt agatctggat gctgtgctgg agcttccgaa
     5881 agtgattctg atgtattttt ctttccagag caatctggat tatgaacata gtgcgttcct
     5941 gtatgcgtat ctgttaaaac accggaaaga ttatgaggaa ctgtacgaac attacgagcc
     6001 gagaatggag cggttcgtca ttgaccagat ccagaagcag catattaaca gacatctggc
     6061 aatactgtat caggaattcc tgtctccggc cattgtaacg gaagctatgg cgaagccttt
     6121 gtcccgtctg ttgtttgccc atatggtaag agtggatgac agcagaatgc gtaaggtgat
     6181 cgtcagccag ccggggaatc tgatactgtc cgagacgcct ctgcaaaacg gcacggcctg
     6241 ggtggcagtc tacgggaatg attataccat tgcttttgag gatgcatatg gtaacaggtt
     6301 ccttaaaaat gtggaatata ccctggaaaa actgctggta ccggggaaat atctcagact
     6361 tctggaacat tatgtgccgg atacggcaga actggatctg tatttcatgg agaacggcag
     6421 aacagaagag accatatctt ccacaaagct gatgcggatg gcaagactgg tggaaagcga
     6481 tgccgtggag ccgaagctgc gcagtgagat tgcggtacag ctggtacagg cctatttcga
     6541 cgcggataat ttgcaggcac tggatgaata cctgcaggag ctgcaggggg acggctttac
     6601 ggaggaacag cgggaactga tcctgcgttt tctggtgttg cggggaaatt atgagaaggc
     6661 atatgcctgg atcgaagcat atacccctta ttttgtggag gccaagattc tgttgcggct
     6721 tacggacggt gtgatcacgc agtcggtcca tgagggagag gcagtcctgt atgcggcggc
     6781 attgacggtg ttccgcaagg gaaaatacaa cggtggaatt ttagagtatc tggtgcgtta
     6841 tgccaccggt accacgaagg aattgcgtga catctggaaa gcggcaagat cctttgagat
     6901 agactgctac agcttaagtg aaaagatatt gctgcagatg ctgttctccg gggcatttgt
     6961 gggagagcgg atggatattt tccgttatta tgtatcccag ggagccagac aggagatcga
     7021 ggaagctgtg cttgtacaga gctcttatga ttatttctgc cgggagaaga tcacggagga
     7081 atatgtattc cgggagatcc gtaactgcta tctgcgggga gaagagaccc agcggatctg
     7141 caagctggct tatctgaaat tctatgcgga gaataaggac aagctggagc gggaagatga
     7201 aactctggtc cggaatttcc tggaggaaat gatgaaggac cacattcatc tgaacttttt
     7261 cagggaatat caggactgcc tgccacagct gcaggagatg aaggacaaga ctatcgtgga
     7321 gtatcacaca agaggcggcg tccgtgcaag gattcattat gtgatgatgc atgagaacgg
     7381 gcaggcggag gattacctgt cggaatatat gcaggaagta tacagcggtg tatttttcaa
     7441 ggaattcgta ctgtttttcg gggagaatct tcaatattac attatggaag aatcagagaa
     7501 cgaagaacag ctgacggaga gcggaagcct gcagaagagc gatattatga atgaaagtcc
     7561 ggacagcaaa tacgagatta tcaatgatat gatgatcagc atgacactgc aggacgatac
     7621 gacactggat catctgatcg aggaatatta ccgcagggaa tatctggatc acagattgtt
     7681 cacattgcag taaagataaa aaaagacagc aggagaggag gcggaagaac atgcgactgc
     7741 cgggaagtga taaagttata gtgggttatg acctggggaa agattatgcg cagatcagct
     7801 gctatgtcac aggcagggaa gaagagatca ccacattgtc ttccgttgca ggctcacagg
     7861 tctatacgat tcccctggtc ctgagcaaga gacagggagt caaccagtgg ttctatggaa
     7921 gtgaagccct ccggcatgcg gaggaagaag agggaatcct ggtggagcat cttctgaagc
     7981 tggcaaaaga tggggaaccg gtgcagatcg atggaacgac tctggatccg gtggcactac
     8041 tgactctttt tctcaagcgt agtctgggaa tgctgtccca gatgacttcc acagagcgga
     8101 tcggggccct gatgatcacc tgcgaggaac tggatgcagg aatgctggag gtgctgacgc
     8161 aggcagtgga ggcattacac ctgaagacgg atgctgtctg ttttcagagc catcgggaga
     8221 gcttttatta ttacaatctg tatcagccgg aaaatctgtg gaagcagggg agcgtgctgt
     8281 gcgagtatag ggacagttcc atccagactt attatatgga atgcaactgc catacgacgc
     8341 cggtggtaac gtacatcgaa gagcgggaat tcccttttcc ggtggaaaaa tcggatgaaa
     8401 agttcctgga gatcgccaca cagctatgcg agaaccggat ggtgtcctcg gtatatctga
     8461 tcggggaaga gttttctcaa gaatggatga aagaatcgct acgctatctg tgccgtggac
     8521 ggagagtctt tcaggggaac aacctgttca gcaagggtgc ctgctacagc atgatggagc
     8581 gactgcatcc cagtgaaaac ggacaaaatc atgtctatct gggacaggat aaattgaaat
     8641 ccaatatcgg tatgaaagtg cgcagacagg gagaggaatc ctatcaggca ctgttagacg
     8701 ccggaatcaa ctggtacgag gcgcagaata ccatggagtt ttacctgctg gaaggaagag
     8761 cggtggagat cctgatcacg tccctgacgg gaaagggcag ccggatcgcc agaatcgtgc
     8821 cggaagaatt gcaggaagga atcaccagac tgcggattca ggtggagatg aaggatgaaa
     8881 cacatctgca ggtggaactg gaggatcttg gttttggcag cttccgaaca gctacgcatc
     8941 atatctggaa ggaagaaata gagttgtaga tctgcccggg gcagagaaaa tgaggatgaa
     9001 aaatgaaggc aagtctgtgt gtaggagaat attgcgagaa tgcctataat gtggaaggcc
     9061 tggatatccg ggtctatagt atggaagagt tgtgctattg cctaaaggag aatgcattcc
     9121 tgttagacct gtccatcatg aacgataagc tggtggactg gatcggagaa gaatgtaaag
     9181 tgtgggaact ggcaaaacag ctctacccca tggtacataa gcagggatcc ttaagtgtgt
     9241 tcgtggtgac gatcctgcaa tatgtgggga tgtacgatcc ggaagaaatc ctgcaggtgg
     9301 agcaggtact gaagcaggga gccggactca gcaatctgga gaaacgcaag agccagatcg
     9361 actatatggt ggaaaaaaga aaatatgcgg cagccatccg tggatatgat atgctgctgg
     9421 agacctggaa ccatctggaa caggaaggaa aggaacttcc ggcgggaaaa gtccgggcgg
     9481 cgatcctgca caataagggt gtggcgctga cagggctgat gttttatgac aaggcggcat
     9541 attactttaa cgaagcttgg aagaccgatc cggacagaga acacctggat gcctatctgg
     9601 cggcaaagag aatggaactg acggaggatg cctatgtggc atttgcagcg cagaatccgg
     9661 aaaattatac ggagtccctg gaactggaaa agcggatcga gcaatttgaa cgggaatggg
     9721 aacagcagcc ggagtaccga cagctacggc tgcgtggtga ctggcgggtg aacgacaggg
     9781 tgaaatacga tgcggaaaat gagagactga ctcaggcact gaaaaacagt taccggacaa
     9841 gtgtcagcgt atagagaaga cagcaggaga gaattatggc gtggtggaac cgtttattta
     9901 agaaaaaaga accgatacag caggaagagg actgggaaca gctggtctat acccgcaacg
     9961 gtgtgaattt tgatgaggaa gaccagagaa aacgttacat tgtaaattgc ctggagcaga
    10021 tctcagaggc ttccaaggag atcgacctcc tgacgggaga gtatacgctg gtgacttctt
    10081 atctgacgga tacggatgag attgaggccc tgccagcaga gcagagagaa gaggtcaacc
    10141 gggtggccgg caagctggcc gggctggaac aggaacgcag cagataccgg gaaaagaaga
    10201 accgtatgcg ggatgcggac ttctatgcaa tccgcaaaag agagaacgag attgaagaag
    10261 gcattcagaa gattcgggac ggcgagaaat acggcaatct gataaggcag gatctgcaca
    10321 gactggacgg agaacgacat gcctacgaat accggagaaa tgagctggag aatctgatga
    10381 acaaccaacg gggcatggct gtcattttcc ttacggcatt gattgtctgt gtgatcatgc
    10441 tggtgatcct gcagtatgct tttgaaatgg atgtctttgt gggacttttt ctggcagtag
    10501 gtgcaggagc tgtagccatc tcctatgtct gcatccggta catggatgca ggacgggaac
    10561 tgcagcgggt ggagaagacc atcaacaaga tcatccagct gcagaataaa gtcaagatcc
    10621 gctatgtgaa caactgtcag ctgatggaat atctgtatct gaagtacaat acggacagtg
    10681 cagcaaagct ggaaaaacag tggaaaatgt atcaggacga aaaggaagag cggaagcagt
    10741 tcgcagaggc ggaagctaag atcgagtact accagaagca gctggtacag cagctggccg
    10801 gttaccgtgt gcagatgccg gagcgctgga tcagccagac agctgcgctt ttggacaagc
    10861 gggaaatggt ggagatccgt cacgaactga tccagagaag acaggtgctc cgtaaacaga
    10921 tggactacaa tcaggaagta gcggagagtg caagaaacga agtaaaagac atcgcagcca
    10981 cctatccgaa atatgcccag gagatcctgg ccatggtgga gcggtacgat cgataaagaa
    11041 ttgacaagac acctctcgtt gtgttactat gacaccgtgt tagcaattta ccttgctaaa
    11101 gcatacgacg cggccaacag tgagtggcag aacgggagga tgtattatga aaagagtatt
    11161 aatggcatta cttgcaatca gcatggcagc aggtgttctt accggctgtg gttctaaagg
    11221 atctgagaaa acatttaccg taggtttcga tgcggaattt cccccttacg gatatcgtga
    11281 tgacagtgga gaatatgtag gctttgacct ggatctggca gcagaagtat gcaagagaca
    11341 gggctgggag ctggtaaagc agcctatcga ctgggatgcc aaggatatgg agctttcttc
    11401 cggagccatt gactgtatct ggaacggttt caccatgaac ggcagagaag atgcctacac
    11461 ctggacagag ccctatatcg acaacagtca ggtattcgtc gtagcgtctg cttccggtgt
    11521 acagaacaag gaagaactgg caggcaaggt ggtagcagta cagaaggatt cctctgcgct
    11581 ggctgctctg aacgatgaag aaaatgcaga caacattgca ctgcgtgatt ccttctccca
    11641 gctgatcgaa tatgcggact acaataccgc tttcatggat ctggagcagg gtgcggtaga
    11701 tgccgtagcc attgatatcg gtgtggcaca gtatcagatt gcgcagagag aagaaggaaa
    11761 attcgttatg ctgcagggcg aggacaacaa gctggctgta gagcagtatg cagtaggttt
    11821 cctgaagggt aatgatgaac tgagagatac cgtacagaag acactggacg aaatggcagc
    11881 ggatggtacc ttcgcacaga tcgctgaaaa atggggactg accgacagcg tatgtctcgg
    11941 taaataatca actaaggata gttaaggagt agaattcatg agtttgctga ccatgagtgc
    12001 ccaactggca gagggctttc tgataacggt ggaaatattt gtactgaccc ttttgttttc
    12061 ccttcctctg gggctgatcg tagccttcgg aagaatgtca ggatgtaagg tgatccgcat
    12121 tatctccaaa atatacatat ccatcatgag aggaacccct ctgatgttac agatcatcgt
    12181 ggtgtatttt gccccctttt atgtattccg catgcaggtg ggaacggggt atcgtttccc
    12241 ggcggtgata ctggcattcg tgatcaatta tgcggcatat tttgcagaga tctaccgtgc
    12301 gggcattgag tccataccgg tggggcagta tgaggctgcg gaggtgctgg gctacagcaa
    12361 ggcacagact ttcgtgaaga tcattctgcc gcaggtggtg aagcgggttc tcccgccagt
    12421 gaccaatgag accattacgc tggtcaaaga tacctccatg gcatttacca tttccattgc
    12481 ggaaatgttc acggtggcaa agcagatcgg agcggcacag accagtgtgc ttcccctgtt
    12541 ggcagcaggt gtgttctatt atatcttcaa tctcgtggtg gcttctttca tggagtatct
    12601 ggagaagaag acatcttatt acagatagga gcagtgcgga aatatggtcc ttttggaaat
    12661 gaatcatgtg aaaaaaagtt ttgacggcaa cggggtgctg aaggatatat ccctgaaagt
    12721 taacgaagga gaagttgtct ccatcatcgg cccctccggt tccggcaaat ccacattgct
    12781 tcgctgtgca acgcttctgg agaaaatgga cggtggtgag ctgatctatt tagggcagac
    12841 ggcagcttcg gagaaaacgg ataaaaacgg cagaatacag tgcgaatatg cgtccaaggc
    12901 agaattacat aagatccgga aatgtttcgg actggtgttc cagaatttta acctttttcc
    12961 ccattatacg gtgatgaaaa atatcatcga tgcgccgatc catgtggata aggtaagccg
    13021 cacagaagcc gtggcgaggg cggagaagct tttagcacag ttgggactgt cggataaggc
    13081 agatgcctac ccttaccagc tgtccggcgg acagcagcag agagtatcca tagccagggc
    13141 actggcactg cagcccagga tcctgttctt cgatgagccg acctcggcac tggatccgga
    13201 attgacggca gaagtattaa aggtgatcag acagctggca agtgaacata tgaccatgat
    13261 catcgtaacc catgagatgc agtttgccag agaagtatcc gaccgcatta ttttcatgga
    13321 acagggtgtg atcgtggagg aaggttctcc cgaagcactt tttacaacgg aaaatgcccg
    13381 ggttcgggaa tttatcggaa agttgcagta aaaatatatt gatttttttt cgcaaatctg
    13441 tttatctatt aggtgtgggt gtatcttgcc cacacctgtt tcaataaaag actgaaaaac
    13501 ataagaatgc gaggactgat ggtatgagcg tacaggaaat gaaaccgatc aaggaaggca
    13561 aagttcgtga gatctatgac aacggagaca ccctgatcat ggtggcaact gaccgtatca
    13621 gctgctttga tgtgatcttg gacaacacag taacaaagaa aggaactgta ttgacccaga
    13681 tgtcgaaatt ctggtttgat atgactgagg atgtgattcc caatcatatg gtatccgctg
    13741 atgttaagga tatgcccgaa tttttcagac agccgaaatt cgacggcaac agcatgatgt
    13801 gcaagaagct gaacatgctt cccatcgagt gcatcgttcg tggatacatt accggaagcg
    13861 gctgggaaag ctacaagaag aatggtaccg tatgcggtat caagcttccg gaaggtctga
    13921 aggaggcaga taagcttccc gagcccatct ataccccttc caccaaggca gagatcggag
    13981 atcatgatga gaatatctcc tatgagcaga gtgtggaata tctggagaag cgtttccccg
    14041 gcaagggcgc agaatatgcg gccaagcttc gtgactatac catcgctatt tataaaaaat
    14101 gtgcggatta tgcactgacc aaaggaatca ttatcgcaga taccaagttc gagttcggac
    14161 tggatgagaa cggcaacatg gtactggggg atgaagtatt gactcccgac agctcccgtt
    14221 tctggccggt agacggctac gagcccggac atggccagcc ttcctttgac aaacagttcg
    14281 cccgtgactg gctgaaagcc aaccctgaaa aaggctggaa gctgccggag gacatcgtgg
    14341 agaagaccat tgacaaatac cttcaggctt acaagatgct gacaggcaag gatctgtagg
    14401 gtacggtttt ttagtttttt ttcacggggc aggcaggaaa ggtaaggctt acaagtgacg
    14461 gacaacattt atattcagac agaaaataat gacaaattaa aggaagaatg tggcgttttc
    14521 ggcgtgtatg atttcgacgg acacgacgtg gcatccacga tttattacgg ccttctggca
    14581 ttgcagcaca gaggccagga aagctgcggt attgccgtca gtgacacgaa tgggcccaag
    14641 ggcaaagtct taagcagcaa agacatggga ctggtcaatg aggcatttac accggaacag
    14701 ctggagaagt taaaaggtga catcggtgtg ggacatgtcc gctattctac agcgggagga
    14761 tccaccagag agaatgcaca gcctctggta ctgaattatg taaagggtac tctgggactg
    14821 gcccataacg gtaacctgat caatgcgccg gagcttcgaa gagaactgga gtataccggt
    14881 gctattttcc agaccaccat tgattccgag gtgattgctt atcatattgc cagagagcgt
    14941 ttgaattcca aatcggtaga ggaagctgtg ggcagagcca tgacgaagat caagggagcc
    15001 tattccctgg tggtgatgtc tcccagaaag ctgattggcg ccagagatcc ttatggattt
    15061 aagccgttat gcatcggcaa gagagacaat gcgtattttc tgtccagcga gacctgcgct
    15121 ctggatacgg taggtgccga gttcgtccgg gatgtgcttc ccggcgagat cgtgaccatt
    15181 tccccggaga aaggaattga gtccgatacc acccatgtat taaagcctgc ggagaccgcc
    15241 agatgtattt ttgagtatat ttatttcgcc agacccgaca gctttatcga cggcgtcagc
    15301 gtatacgatt cccgtattct ggccggaaag ttcctggcta tggattcccc ggtggatgcg
    15361 gatctggtca tcggcgtacc ggaatccggc aactgcgcag ccatgggata ttccatgcag
    15421 tcgggaatcc cttacggcat gggcttcgtg aaaaatgcct atgtgggcag aaccttcatc
    15481 aaaccgaagc agaagagcag agaaagcagc gttcaggtca aactgaaccc tatccgggaa
    15541 gccgtggagg gcaagcgtgt catcatgatc gatgattcca ttgtccgcgg caccacctcc
    15601 gaccgtatcg taaagatgtt acgggaagcc ggagccaaag aggtacatat gagagtcagt
    15661 tcaccaccgt ttctgtggcc ctgctatttc ggcacagacg tcccggcgag agaccagctg
    15721 atcgcatata accgttccat caaggagatc agtgatctga tcggcacggt cagcctggat
    15781 tatctgaaga ttgagagact ggaagaactg gtgggcggta atcttggcat ctgcaagggc
    15841 tgctttaccg gaaaataccc gatggaaccg cctgcggaag atatccgcgg agaatttgac
    15901 aaataaacaa taactattcg gaaaaccgca gaataagaaa aactgatacg tcatgcctgc
    15961 atgtaagtag cagttttctt atttgtaagg tgactcgagt agagtcacca tcccacacat
    16021 gaacaaaatt agctgcgaag cagcgggaaa gtatgagaag catatggttt tgtgaatgtg
    16081 agggatgggt tttgaatggt tgcaataaat tagaaaataa gaggagcaga caaatgagta
    16141 caggtacaga cagatatcag agcccgttat ccgaacgcta tgcaagcaag gagatgcagt
    16201 atattttctc cccggatatg aaattccgta catggagaaa gctgtggatt gcactggcag
    16261 agacggagaa agagctggga ctgtcccagg acggtaagcc cgttatcaca gatgagcaga
    16321 tcgaggaact gaaggcccat gctgaggaca ttaattacga tgtggcaaaa gagcgtgaaa
    16381 aactggtacg tcatgacgtt atgagccatg tgtatgcata cggacagcag tgccccaagg
    16441 cagccggaat catccatctg ggcgcaacct cctgctatgt gggtgacaac accgacatta
    16501 ttgtgatgac cgaggcactg aagcttgtca gaaaaaagct gatcaatgtt atgaaggaac
    16561 tggcggattt cgctgataag tataaggcac agcctaccct ggcatttact cattttcagc
    16621 ctgcacagcc tacaaccgta ggcaagagag caacgctgtg gttacaggag ttctcactgg
    16681 atctggagga tctggatcac gttctgggta ccatgaagct gttaggctct aagggaacca
    16741 ccggaaccca ggcttctttc ctggaactgt tcaacggaga tcaggagacc atcgacaaga
    16801 tcgatcctat gatcgcagcc aagatgggat tcaaggagtg ctatcctgta tccggccaga
    16861 cttattcccg caaggtggat accagagtag ccaacgtgct ggccggtatt gcggcatctg
    16921 ctcataagat gagcaatgac atccgcctgt tacagcatct gaaggaagtg gaagagccct
    16981 tcgagaagaa ccagatcggc tcctccgcca tggcttacaa gcgtaatccc atgagaagtg
    17041 agcgtattgc ttccctgtcc cgttatgtga tggtggatgc gctgaatccc gccattactt
    17101 ccgcaaccca gtggttcgag agaacgctgg acgattctgc caacaagaga ctgtctattc
    17161 ccgaaggctt tcttgccatc gatggtattc tggatctgtg cctgaatgtg gtagacggac
    17221 tggtagtata tccgaaggta attgaaaagc acatgatgag cgagcttcct ttcatggcta
    17281 ccgagaatat tatgatggat gccgtaaagt taggcggcaa ccgtcaggaa ctgcatgagc
    17341 gtatccgtga gctttccatg gaggccggac gcaatgtgaa ggtagaagga aaagagaata
    17401 acctcctgga gctgatcgcc gcagatcccg cattccccat gggactggag gaactgaaag
    17461 catccatgga tccgtccaag tatatcggac gttccaaaga gcaggtggaa gctttcctga
    17521 aaaatgtgat cagcccgatc ttagaggcca acaaggactt actgggtgta aaggccgaga
    17581 tcaacgtata aacgttttct gctgaagtaa tttgtggaaa cgaaaactat tcagaacctc
    17641 attcatcggt gatcttttga tgtttacatg gctctgaatc gttacagaat gataagaaag
    17701 caggagacga aaatgggtgg attttttgga gtcgcagcaa aagaagactg tgtgtttgat
    17761 ctgttctttg gaacggatta tcattcccat ctgggaacca gacgtgccgg catggcagtc
    17821 tacagcaaag aagaaggtta caacagagcc attcacaaca tagagaatgc accgttccgt
    17881 acgaagttcg acaaggatgt caatgagatg cacggacatc tgggcatcgg ctgtatttcc
    17941 gattttgagc cccagccgct gatggtgcgt tcccatcacg gaacttatgc tattaccacc
    18001 gtgggtaaga tcaacaatac ggatgccatc atcaaggatc tgtttgcgaa gggacattct
    18061 catttcctgg agatgagcgg cggtgacatc aatgccacgg agctggtggc ggccattgtc
    18121 aaccagaagg acaatctggt ggagggcatt cagtatgccc aggagatcat cgacggttcc
    18181 atgaccttac tgatcatgac acccaaggga atctatgcgg ccagagataa gatgggccgt
    18241 actccggtgg cagtaggcaa aaaggagggg gcttattgcg tctcctttga gagcttcgct
    18301 tatctgaatc tgggatacca gcccgtgaga gagctgggac cgggagagat cgccgtggtg
    18361 acaccggaag gtgtacgaac cctggtgaag cccggcaagg atatgaagat ctgtacgttt
    18421 ttgtgggtct attacggata tccgtcctca tcctatgaag gcatcagtgt agagaagatg
    18481 cgttatcaat gcggtgccaa gctggcggag cgtgataatg taaaaccgga tatcgtagca
    18541 ggagtgccgg attccggtac cgctcatgca gtgggatatg ccaaccgttc gggaattcct
    18601 ttttcccgtc cgtttattaa gtataccccc acctggccca ggtcctttat gccgacgatc
    18661 cagacccaga gaaatctgat cgcaaagatg aaactgatcc cggtacatga tctgatccag
    18721 gatcagagtc tgttgttgat cgacgattct attgtccgcg gcacacagtt aagagagacc
    18781 acggaattcc tgtaccagag cggtgcaaag gaagtacata tccgtccggc ctgtccgcct
    18841 ctgttatatg gctgcaagta cctgaacttt tccagatctt cttccgagat ggatctgatc
    18901 acccgccgtg tgatcaagga gattgagcag gagggcagag aaatcgatct gaagaactat
    18961 gtgaatcccg atactccgga atacgaggag atggtaggac ggatctgcaa acagctgaag
    19021 ttcaccacat tgcagtttca gagattggat gatatgattg agtccgtggg catcggcaga
    19081 gagaagctgt gcacctactg ttgggacggc agagaataat gcgaaaagaa cttctaatca
    19141 ctcagagcat aggctctttc gtgaagaagt tctaagtttc gcatggcaaa acgctacgcg
    19201 ttttggatat agatgtaggt tatataaaat ataataaata ttgattttac ttataccaca
    19261 aagtgttgta tactgtaaaa cggtgtgaga aagagaagca ggagtttctt tggatcaccc
    19321 ggaataagat attggatgta gatttatcac agaaaccgtg gtaaagaatg gaggaaaaca
    19381 tggtaaaggc agttgtagga gccaactggg gcgacgaagg aaaaggaaag atcacagata
    19441 tgctggcaga gaaagcggac attatcgtac gttttcaggg cggcgcaaat gcaggacata
    19501 ctatcgtgaa caattatggt aaatttgctc tgcacacact tccttccggt gtattttaca
    19561 atcataccac cagcgtgatt ggtaacggtg tggcattaaa cattccgatc ctgatgaaag
    19621 agatcaagtc tatcacagac agaaatgttc ccatgcccaa gattctggtg tctgatcgtg
    19681 cacagatggt aatgccttac catattttat ttgatcagta tgaagaggag agactgggcg
    19741 gtaagtcctt cggatctacc aagtccggta tcgcaccttt ctattctgat aagtatgcga
    19801 agatcggttt ccaggtaagt gagctgtttg atgaagaact gctgcaggaa aaagtacagc
    19861 gtgtcagtga gatgaagaat gtcatcttag agcatctgta tcacaagccc ctgatcgatc
    19921 ccgcagagct gttaaatacc ctgcatgagt atcgagatat gatcgcacct tttgtatgcg
    19981 atgtatccgt attcttagat aaggcactga aagagggcaa gaccattctg ttagagggcc
    20041 agttaggaac catgaaggat ccggatcacg gaatttaccc catggtaact tcttcctcca
    20101 cgttggcagc ttacggcgcc atcggtgcag gtatccctcc ttatgagatc aagcagatcg
    20161 ttaccgtaag taaggcatat tcctcttccg taggagcagg tgctttcgta tctgagatct
    20221 tcggtgacga agcagatgaa ttacgtcgcc gcggcggtga cggaggtgag ttcggtgcaa
    20281 ctaccggacg tcccagacgt atgggatggt tcgactgtgt tgccaccaag tacggctgtc
    20341 gtctgcaggg cgctaccgat gtggcattta ccgtactgga tgcgctggga tatctggatg
    20401 agattcctgt atgtgtaggg tatgagatcg acggtgaagt taccactgat ttccctgtta
    20461 cctacaagct ggaaaaggca aagcccgtac tgaaaaagct ccctggctgg aagtgcgata
    20521 tccgcggtat ccgcaacttc gatgaactgc cggagaactg cagaaagtat gtggaattca
    20581 ttgaggagca gatcggattc cctatcacca tggtcagcaa cggcccggga agggaagata
    20641 tcatctatcg tgagagtcct ttgaagaaat aaatgctgtc aaatgcggag agataggata
    20701 atctttgatg gaaaaaacat ctgtaaaatg tgaatgagaa tggaaagggc atggtgctgt
    20761 taagagataa gcccgtgtcc ttattagtaa acgataaatc cggcaggaat attgattaca
    20821 cagaatcagg ctgaatgcaa gcattcgcag aacgcagttt tacatggctc tgaatagcta
    20881 cagattttta ctgtgaagga acaaaaacca gtgtctgata gtaattttgg gatggagaca
    20941 gcgagatatg atcaataatc tggaagcgta taaagtattt tattatgtgg caaaatgtgg
    21001 gagtgtgacc aaagcggccg gggaactgtc aatttcacag ccggcggtca gtcaggccat
    21061 caagcagctg gaaaataccc tggatgccgc cctgttccac cgggcggcca agggagtacg
    21121 gctgaccagt gagggagaac tgttgtattc ctatgtggca aaagggtacg aacagatcga
    21181 aatgggtgtg aagaaggttc accagatgca gaatatggaa ctgggagaag tccgcatcgg
    21241 tgccagcgat atgacattgc agttttatct gcttccctat ctggaaaagt tccatgaaca
    21301 gtaccccggg atcaaggtga tcgtcaccaa tgcgccgaca ccggagacat tgaattattt
    21361 aagagaggga cacattgact tcggaattgt cagtacgccg tttccggaga gacctgatat
    21421 tcagatgata ccggtgcggg agattgagga tgtcttcgtg gcgggaagaa gatttttcag
    21481 ctacaagaac cggacgctgg attttcagaa actggaaagt ctgcctctta ttttcctgga
    21541 gaaaaatacc agcagccgca gctacatgga tgaatttctg gcgggaaacg gggtatatgt
    21601 gcagccggag ttcgaactgg ccaccagcga tatgatcgta cagtttgtga gacgaaatct
    21661 gggtgttggc tgtgtgatgc gggattttgc cagagattat gtggaatccg gcgtgctttt
    21721 cgaacttaga ttcaataaaa taataccgaa gcgtcaattc tgtgtcgtta ggaacacgaa
    21781 gatgcctatg gctccggcgg cggagaaact gttggagatc atggagctgg acagacagga
    21841 agaagcgaag atataaaagt gacggcaaaa agaggatata ccggcggaaa cggaactgct
    21901 ttgactagta aaacgaatag gagagaagag ttatgatacg caatataatt tttgacattg
    21961 gcaatgtgtt gacagactat cgctgggagg gatttctggc ggacaaggga tttgatgcgg
    22021 aaatgatcaa aaaaatcgca aaggcatcta ttatgcatcc gctgtgggca gagtatgacc
    22081 gcggtacctg gacggacgag caggttctgg ctgcatttgt aaaggatgca ccggaactgg
    22141 agaaagaact gcatcaggcg ttcgagaata tcgctggcat ggtaaccccc agagcttacg
    22201 ctattccctg gttacaggaa ctgaaagcaa agggatacca tgtgtattat ctgtccaact
    22261 tctcacacaa ggcagagaca gaatgtgcgg aagcgttgaa tttcctgccg gagatggaag
    22321 gtggcatcct gtcctacaag gatcagctga tcaagcccga accggagatt taccagctgc
    22381 ttctgaaacg ctatggatta aaggcagatg agagcgtttt cctggatgat acgctggtga
    22441 atgtgaaggc ggccgaagaa cagggtatcc acggcatcca cttcctgacc aaagagcagg
    22501 cggaagagga actgcgtaag ctcggcgtgg actgctaaat ggttcgtagc ggagggtgcc
    22561 ttagaccgga cgaactccca ga
//
LOCUS       DAWDHD010000025        22225 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_169_length_22225_cov_13.615246, whole genome shotgun sequence.
ACCESSION   DAWDHD010000025 DAWDHD010000000
VERSION     DAWDHD010000025.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 22225)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 22225)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..22225
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_169_length_22225_cov_13.615246"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         6855 a         4332 c         5610 g         5428 t
ORIGIN      
        1 tgattgatac cgccttgtct atggtatata ctatggatat accgtatgca aggagacatt
       61 ttttatgata atctatgatt catcatagat tatcataaaa aatattgatc aaaaaataga
      121 gacaataaaa gagtgagtta tatcatggat acaatcagag gtaaattttt caaacaacat
      181 ttattatcag atcaattcct cgatctgatt gagaaaaaca agcgccccat ggacgaactg
      241 atgtcgtatt atcagtgcgc catcatggag gtggagacga aattcaaggt tttgaatcag
      301 gagtattccc tggaatacga ccgcaatccc atcgaaggga tcaagaccag agtcaaatcg
      361 tatgacagta ttctgcgtaa gatccgtcgg aaaaatattc ccatgacgct ggaaggcatc
      421 gaagagaata tccgggatat cgccggagtc cgggtcatct gttccttccc ggatgatatt
      481 tacgaactgg cggagagttt cttacggcag gatgacatta ctctgatcga gcgcaaggat
      541 tatataaaga atcccaagga gagcggctac cggagcctgc atctgatcgt gcaggtgccg
      601 atctttttac agaatacgaa gaaactggtc tatgtggagg tgcagttccg caccatagcc
      661 atggatttct gggcaagcct ggagcacaag ctgcagtata agaaaaacat tccggaaagt
      721 cagtcgaagt ttttaaagga tgagctgtat gactgtgcgc agcagagcgc agcgctggat
      781 aagcggatgc agaatatccg gaatgtcatt gcggcgagtg agaccgcaga agaggagaaa
      841 caggatttcc tgccaatctt tttgcgggaa aacaaagggt aattacgtag cctgaaggta
      901 tgtagatgat ggaaaggcga gaagaattgt atgaagatca gagatatttt gaataaagat
      961 acagcaacgc tctcttttga ggtatttcct ccgaagaaga gtacggattt cggaaatgtg
     1021 gaggcagcag cactggggat cgcggcattg cagcctgctt atatgagcgt gacttatggt
     1081 gccggcggta gtaccaaggg acataccatt gccttggccg gagatattca gaagcagtac
     1141 aatgtgccta ccgtagcgca tctgacctgt gtctgtgctg acaggagcag tatcggcgca
     1201 gctcttacag aaatgcaggc agcagggatc gagaatattt tagctctgag aggagatatt
     1261 ccgaaggatt ttgatgggga ggtatttaca gattttaccc atgcatcaga tctggtacgc
     1321 tttatcaaag agaacggaga tttctgcgtg ggtggtgcct gctatccgga gatgcatccc
     1381 gattctgcga ataagaatgc ggaaattcag ggactgaagg aaaaggtgga tgccggatgt
     1441 gagtatctga cgacccagat gttcttcgac aacaatatct tcttcaactt tatgtaccgt
     1501 gtgcgggagg cagggattac agtgcccatc attcccggaa tcatgcctat cacccgtggc
     1561 gtacaggtga aaaatgccgt aaaactctcc ggctgtaatg tgccggagcg ctttaagaat
     1621 attgtggacc gcttcgggga taatccggaa gccatgaagc aggcgggcat tgcctatgcc
     1681 accgatcaga tcatcgacct gctggcgaac ggtgtgaaac atatccatgt ctattccatg
     1741 aataaaccgg atgtagcggc cgggatccag cggaatttgt cggagatttt gaaagcgtaa
     1801 agaatttcta ctttggtaga ttaaagctgt caggttaaaa agaaaatata tgctataaac
     1861 acaattaaat aaaatgatga ttaaacgctt agggaatgtt ctctgagcgt ttttatttga
     1921 aaaaataaca taacacagct ttacaaaaaa tatagtagag gatataatta gatataagat
     1981 tttacaacaa ggggaggaaa atatatgaat tataaaacag gccttgaaga ttttattgga
     2041 aaagaatcct tatcaaagac attaagaaat gcattgattc ctacagagag tacaaagatc
     2101 catatggaag agatgggggt catccgtgat gatgaattgc gagcagaaaa acaacaggaa
     2161 ttaaaggaaa tcatggatga ctattataga acatttattg aggaaaaact tggtcaaatt
     2221 caaggaatac aatggaattc cttattccaa aaaatggaag aaacgatgga ggacatatca
     2281 gtacgaaaag atcttgataa aatacaaaac gaaaaaagaa aagaaatctg ttgctatttt
     2341 accagtgata aaagatttaa ggatctgttt aatgctaaat taattacgga tatccttcca
     2401 aactttataa aagataataa agagtatact gaggaggaga aggcagagaa agagcagaca
     2461 cgggtgttat ttcagcgttt tgctactgca tttactaatt attttaatca gcggagaaac
     2521 aatttttctg aagataatat ttctacggcc ataagctttc ggattgtaaa tgaaaattcg
     2581 gaaattcatt tacagaatat gcgggcgttt cagcgaatag agcaacaata cccggaggaa
     2641 gtctgtggaa tggaagaaga atataaagat atgttgcagg aatggcagat gaaacatata
     2701 tattcggtag acttctatga cagagaatta acacagccag gaatagaata ttacaatggc
     2761 atttgcggga aaataaatga acatatgaat caattctgcc agaaaaatag aattaacaaa
     2821 aatgattttc gaatgaaaaa gctacataaa caaatactat gtaaaaaatc ttcctattat
     2881 gagatcccgt ttagatttga atcagaccag gaagtatatg atgccttaaa cgaatttata
     2941 aaaacaatga aaaagaaaga aattattcgc agatgtgtac atttagggca agaatgtgat
     3001 gattatgacc tggggaagat atatattagt agtaataaat atgagcagat atcaaatgcg
     3061 ctatatggaa gctgggatac tataagaaaa tgtataaaag aagaatatat ggatgcgctg
     3121 cccggtaagg gggaaaagaa agaagagaaa gcagaagcag ctgcaaaaaa agaagagtat
     3181 agaagtattg ctgatataga taaaataatt tccttatatg gatcggaaat ggatcaaaca
     3241 atatcagcaa aaaaatgtat tactgagatt tgtgatatgg caggacaaat ttctatagat
     3301 ccgctggtat gcaatagtga cataaaattg ctgcaaaata aggagaagac aacggaaatc
     3361 aagacaatat tggattcctt tttgcatgta tatcagtggg gacagacttt tatcgtaagc
     3421 gatataatag aaaaagatag ttatttttat tctgagttag aagatgtttt ggaagacttc
     3481 gaggggatta caacgcttta taatcatgtt cgaagctatg ttacgcagaa gccatatagt
     3541 acagtaaaat ttaagcttca tttcggttct cctacattgg caaacggatg gagtcaaagc
     3601 aaagagtacg ataataatgc aattttgctg atgcgggatc aaaaatttta tttgggaatt
     3661 tttaatgtaa ggaataaacc ggataaacaa attataaaag gccatgaaaa agaagaaaaa
     3721 ggagattata aaaaaatgat ttataatctt cttccggggc cttcaaaaat gttgcctaag
     3781 gtttttatta cttcacggtc aggtcaggag acatataaac cgtcaaaaca cattttagat
     3841 ggatataacg aaaagagaca tattaagtca tcgccccaat ttgatttggg atattgctgg
     3901 gatttgatta actactataa agaatgtatt cataaacacc ctgattggaa gaattacgat
     3961 tttcactttt cagataccaa agactatgaa gatatcagcg gattttacag agaagtagaa
     4021 atgcaggggt atcaaattaa gtggacgtat attagtgctg atgagattca aaagctggat
     4081 gagaaggggc aaatattttt atttcagata tataataaag acttttccgc tcatagcaca
     4141 ggaaaagata atctgcatac gatgtatctc aaaaatttat ttagtgaaga gaatctcaaa
     4201 gatattgttt tgaaattaaa tggagaggca gaattatttt tccgaaaagc aagtataaaa
     4261 actccggttg tacataagaa gggatctgtt ttggtaaata gaagttatac acagacggta
     4321 ggtgataaag aaatcagagt ttccataccg gaggaatact atacagaaat atacaattat
     4381 ttgaaccata taggaaaggg aaaattgtct tcggaagccc aaagatatct cgatgaaggc
     4441 aagattaaaa gcttcactgc gacaaaagat attgtaaaaa attatcgcta ttgttgtgac
     4501 cactattttc tgcatcttcc cataaccatt aatttcaagg caaaaagtga cgttgcagta
     4561 aatgaaagga ctttggcata tattgctaaa aaagaagata ttcatattat tggaattgac
     4621 agaggggaaa gaaatctact gtatatttct gtagtagatg tgcatgggaa tatacgggag
     4681 cagcgttcct ttaacattgt taatgggtat gattatcaac agaagttaaa ggatagggag
     4741 aagagcaggg atgcagcaag aaaaaattgg gaagaaatag aaaaaatcaa agagttaaaa
     4801 gaaggctact tatccatggt aattcattat attgcgcaat tagttgtgaa atataatgcg
     4861 gttgtagcca tggaagactt gaattatgga tttaaaacag gaagatttaa ggttgagagg
     4921 caggtttatc agaagtttga aacgatgtta attgaaaaat tacattatct ggtatttaaa
     4981 gacagagaag tatgtgaaga aggaggagtg ttaagaggtt atcagttaac ttatattccg
     5041 gaaagcctta aaaaagttgg gaaacagtgc ggatttattt tttatgttcc tgctggatat
     5101 acttctaaaa ttgatccaac aacaggattt gtaaatcttt tttcatttaa gaatctgaca
     5161 aatcgagaga gcagacagga ttttgtaggg aagtttgatg aaataagata tgacagggat
     5221 aagaaaatgt ttgagttctc atttgactat aataattaca taaaaaaggg aacaattttg
     5281 gcttcaacaa aatggaaagt gtatactaat ggtacgagat taaagaggat agttgtcaat
     5341 ggaaaatata caagtcaatc gatggaggta gaacttaccg atgctatgga aaaaatgttg
     5401 cagagggcag ggattgagta ccacgacgga aaagatttaa aaggtcaaat tgttgaaaaa
     5461 ggtattgaag ccgaaattat tgatattttt cgactcacag ttcagatgcg gaacagccga
     5521 agtgagtcgg aagacagaga atatgacagg ttgatttctc cagtattaaa tgataaaggc
     5581 gagttctttg ataccgcaac agcagacaaa actttgcctc aggatgcaga tgcaaatggt
     5641 gcatattgta ttgctctgaa aggtctttat gaagtaaaac agattaaaga aaactggaag
     5701 gaaaacgaac agttcccaag gaacaaactt gtgcaggata ataaaacatg gtttgatttt
     5761 atgcagaaaa aaaggtattt gtagatggaa gattttttgc ccggcctcca catattttca
     5821 taaattatat ggaggtcagg acaacctgac ctatcagacc gaagcgcaga tgaacggaac
     5881 aaatgcccat aaagtgattc ggacatagga tacaatattc tgtgtttgaa attgacaata
     5941 gtgatcgaat aaaggataat ataattacag aaaatacaaa cctagcctca aatatctgta
     6001 gataggttta ttgtaaaaaa aattagtgaa ataataacaa aaaagtgcat aaaacataag
     6061 cacattaagt cccaaattgt gcaataacac aaaataagag ttaaataata agaaagaatt
     6121 tctactagtg tagatcacgg ttgactgata gatgtgtcga tagttaaata ataagaaaga
     6181 atttctacta gtgtagatgt gaaacaccct atattgctgt cacggttaaa taataagaaa
     6241 aaatttctac tagtgtagat ttaagtggtg cttagtgatt tttttattgt taaataaaat
     6301 aataagaaag aatttctact agtgtagata tgtgggtata gcctatgtgg ttaccgggtt
     6361 aaataataag aaagaatttc tactagtgta gatcggtttt tttaacatat atagatttaa
     6421 ataaccagaa acacaacatt aactcgtcaa ataaatcatt gacagagcaa aaaagatgta
     6481 ctatcatcca ttttggatgc aggtacatct ttttctgtta tctgaataca acacagagag
     6541 gtagaacaat ggatgcaaca gaacgaaaaa tgacgaaaat agcaagagaa gtgagcaaat
     6601 tcacggtgca gaccatgaag gaagatggga ttgggactgc ggaatttgat tttatccatt
     6661 tggtacggca caatccgggc attacccagg cggcggtgcg ggaacagctg aagatcgaca
     6721 agggagctgc cgcaaggcgt gcggcgagtc tggagagcaa gggatacctt taccggaagc
     6781 ccaacccggc ggatggcagg agccagctgt tgtatgccac ggagaaggcg gagcagttga
     6841 aaaactccaa ggctgccatt gagagcaaat tttatgaatg gctgttggcg gaacttccgg
     6901 aggaagagaa ggaagcattt tgtaagacgc tggataccct gtactggcgc accaagcagg
     6961 aacgtcgggc aggatttgtc aatgtggcaa aatgcgtgga agaaggagca ggagaggatg
     7021 aaacagatac cggagacagt gaaaaaggat cagatcctgt attatttcaa aacccagtgg
     7081 cgggtgctcc tggcggtgac gttttccgga ctgatctata acgtaggtct tctggtcggc
     7141 ccctggtgcg agggacagat gaccggttgc ctggtggaca ttctgggtgg gaaggccgga
     7201 taccgggata tgctggtgct ggtgaccgca tatgtgatat ctattgccat cgtgcagatc
     7261 tcccgttata ttaaacgttt ttatgtcaga cgatttgcta ataatgtgaa tcgtgacatg
     7321 aaagagaccc tgtaccacag cctggtgcac agaagccgtg ctgaactgga agaagagggt
     7381 gctggaaatg tcatgaccaa ggcaatcctg gatgtggatg actgcgcgga ggggatgagg
     7441 aaatttacca cagagatctt cgataccggt gtggcactgg cggcatatgt gggaatgttg
     7501 ttattctatg actggcggct ggcaatctta ggtatgttgt ttttaccggc gtcatatgtg
     7561 ctggcggaga agatgaagcg taacgtgcaa cgcaccggag ctgcttataa ggagcagtcc
     7621 ggagacttaa gtgccgcaac actggacaga gctgccaatg cggttactta ccgtgtctat
     7681 ggcagagagg aagagcggaa gcaggcctat gaagcgaacc ttaccgctta tgagaaatcc
     7741 gctgtgaagg ccaatatctg gagtactgcg ttccccccgc tgtaccggat catctccatg
     7801 atcggcgtac tgtttatcct gtatttcggc agtagaaatg tgctgggcac cggctggaaa
     7861 tcatgggatg tggcagcgtt taccacgttt ttatcctgct ttttgaagtt agccgtgaaa
     7921 tcttctcatg cagcgaagct attcaatgcg gtacataaag cccaggtctc ctggaagcgt
     7981 atcaaaccac tgatgaagga agacacttat gaggacgaca gcttggtaca gaagccggga
     8041 atgctggaag tatcccatgt aagctttgct tatcccggcg gagagaagat ctatgaggac
     8101 ttcaatctgt ccgctttccc gggacagatc atcggtgtca ccggagcagt ggcgtgtggt
     8161 aagtccacat tgggtaagac cttcctctgt gaataccctt acgaaggaag gatctgtttc
     8221 cacggcaaag aattatccgg gatgaccaag gcagagcaaa acggcatggt gggatattta
     8281 ggacatgatc cggagctgtt caatgacagt gtccgtaaca atatactgct gggcgatgat
     8341 gacgatccgg agaagtatct gaaggcagtc tgtttcgacg gtgaggtggc agagatggaa
     8401 gagggtatgg acaccattgt gggaaatggt ggtgtccgac tctccggtgg acaggcacag
     8461 cggctggcat tggcgagaac cctgtgccat aagcgtccgg tcctgatcct ggacgatccg
     8521 ttttctgcac tggatcgtaa gacggaggaa gaggtcttcg ccaatctgcg gaagtttaca
     8581 gcggacagta tcgtgatcct gctgtcccac agactctatc tgttccccga aatggatcag
     8641 gtgctgtgga tggaggctgg aaaagtgact gcgggaaccc acgaacagat ccttgaaaag
     8701 ttcccggagt atgcgagact gtatgaagca caggcggatg gcgctgatgc acacagtacg
     8761 caggagggag gtctggatca tgcagaaaaa taaacagatg cagaaaaata agaggcagaa
     8821 tgtgaaaaat gtccgcagcg tgatccggca gactgtgaca ggctatgcag ggctgtcact
     8881 gggaatcctg ttggcagtcg caggtgccat cgtaaccgcg cttctgccgc cctggatcct
     8941 cggaagtatt gtggatacga tcaccgcagg aaacacagtt ccggttgcat tggtgatact
     9001 atattttgct tttacggtgc tcaccggtct gaccgaaagc cttcgggaag gtctgctgac
     9061 ggtcttcggt cagaagatca ctcatgcact gcggagcagc atgatggaga aatatacgaa
     9121 cctgacggca ggggaactga cgaagcagga gcccggtact atcgtatccc ggttcgtagg
     9181 agatgtggat acggtggaga acctgttcac ctccggaatc atcagtatgt tcgccgatgc
     9241 ctgtaagatt ataagtatcc tggtggtgat ctggttaaaa aacagaggac tggcgatcgt
     9301 acttttggtt ctgcttccat ttctgtattg gttcacccgg acggtgcaga aaaatatgct
     9361 gaaagcccag atcgagaacc gttgcgccgt gggcagagca tccggacatg tgccggagac
     9421 gctgcacaat atccgtacca ttcatacgct gggaaaagag cgctacatgg agcaacgcta
     9481 tgatgaatat attgcggaga gctaccgcgc ggtagatcgc acgaattttt atgatgccat
     9541 ttattccccg gtgatcctga tcctgaatgc tatcgtggtg gcggttgtga tgctgttttc
     9601 tgcctccggt aatgcaaagg tgctgacatt gttcggtatg tctgcaggta cagcggtagc
     9661 ggtaattaac tatatctcac agatctttac ccctgtggag agtctgggaa tggagatcca
     9721 gaccatacag tccgccattg cgggcgtgca ccggatcaat gaattctatg cgctggagga
     9781 attgccggag agaaattccg gggaaaaacc ggcagaacca cagaatagac cggaaaaacc
     9841 ttttgtggaa ctgcaaaatg tgacctttag ctatgaggat gatgaccgta agattttgca
     9901 ggctttaaat tttcaagtat atcccggaga acaggtgaca ctgtccggaa gaaccggagc
     9961 agggaagagt acggtcttca aactgttgtt gggactctat cagccgggag agggtaaagt
    10021 gctgattcag ggaagagatg cttttcagat tccgaaacat gaaaagcgca gcctgtacgg
    10081 ctatgtggag cagacttttc atagagtacc cggtaccgtg aaggatcaga tcacgctgta
    10141 tgacgattct tttaccatgg aagaggtacg gggggctgca agaattgcgg gactggatgc
    10201 gacgatagaa caattggaaa aaggctatga cactccctgt acccaggaga tcttctccca
    10261 gggacagtgg cagctgttat ctattgccag agctgcggtg gcgaagccga agctccttct
    10321 gttggatgag attacggcaa atctggatgc ccagaccgag gaagaggtgc tgcaggcact
    10381 gaagcgtgcc tcggaaggaa gaacggtaat ctcaatttca catagagtaa atgcggaaac
    10441 aggacggatc attaacatat agtaagatgt aagatgcctg acggattgct ccgtgctgcg
    10501 cactctcaca atcctgcccg gtattcgcat atcgtgaagc tgacgcttcc cttttatgcg
    10561 aatatcggag cgggtcatga aacaagaacc ccacgtttgt gcgagcacag cgtggggttc
    10621 ttgtttatga ccctggcatc ttacatagaa ataagtccaa atgcatttgc cacagagctt
    10681 aataaaataa tcgcagcaaa taccgggcag agatacttga tcatgaaatt gaatatcttt
    10741 ttgcggcgga aagcggcttc tccgtgcatg acttcttctt ccacagcctt tactccgatg
    10801 acgcgggaca ccaacagaca gatggtgatg gcagcgatag gcatcatcac ggagttggtc
    10861 aggaaatcga agaaatcaag gaactgcatg ccgatgatct tcacagcacc aagcggtccg
    10921 taacccagtg aggataacgt tcccagtgcc agcatgatca cacccatgat cagggtagat
    10981 tttttacgac cccagccaag ctcgtcctcg aaagtagaca cagcactttc ggtaagtgca
    11041 atggagctgg tcatggctgc gaacagtacc agtacgaaga acataatacc tacgaaggtg
    11101 cccattccca tgctatcgaa gaccttggga agtgtgatga acatcaggga aggacctgcc
    11161 tgtaaggtat cgggatctcc accggagaat gcgaacacgg cgggaatgat catcagacct
    11221 gccatgatag caatggcggt atcgaagatc tctacatttt tggtggagcc ttcgatggaa
    11281 acatcttttt tcatataaga gccgaaggtg atgagaatac ccatggcaat ggacagagag
    11341 tagaacatct gccccatggc agctacgact gtcatccagg agaaattcgc aggattcggg
    11401 atcaggaaat acttcacgcc gctcagtgct cccggtctgg taacagaata gactgtgatg
    11461 accacggaca gcactaccag gatcggcatc ataatgcggg atacgcgctc gataccgttg
    11521 cggacaccgg cgtagatgat gcccagcgta cacagagtga atacgataaa gcacagctca
    11581 gtagagacac cattggaaat aaacgtggaa aaataaccgt ctgctgccag atcatgtccg
    11641 tgccccttgg catattccac cagatatttg atcacccatc cgccgatgac ggaataataa
    11701 ggaacaatca gaataggaat aatagcgttg atccatccac cgaaggacag ccatttggac
    11761 ttcccgaagg tatgaaaggc ccccacggga cttttacctg tcatgcgtcc cagtgaagtc
    11821 tctgccacga tcatgctata accgaaggtc agcgccagaa taatatagat caacaggaag
    11881 ataccgccgc cgtatttggc tgccagatac gggaaacgcc agatattgcc taatcctacg
    11941 gatgctccgg cagccgacag aacgaagccg atcttgccgg aaaatgaact tctgttatta
    12001 tttttgtgat tcatagttta aactctccca taaaaattgt cgtaaacaga ataagtgtaa
    12061 catagaaata aggaaaatac agtacgaaat agtctgattg agaatggaaa aattcgataa
    12121 tgcaagagta aaaattgaat ggatgccagc aatggcgctg ctgaatgacg gtgtatctgg
    12181 caatttaaag tatatttttt cctgcatttc gcatactaac tgcaacgtta atgattagta
    12241 atgcgagatg atgatttatc ttacgcaata tgtcatggag gaaaaaatga tgaaagcaac
    12301 tggaattgta agaagaatag atgacctggg acgcattgtg atcccgaagg agatccgccg
    12361 cacgttacac atcagagaga gtgatccgct ggagatcttc acagacagag aggggcaggt
    12421 catcctgaaa aaatattccc ctatcggaga gatgaccaca tttgccaaac agtatgcgga
    12481 gagcctggca caggtgtcgg gacatgccac actgatcgcg gacagagacc agttcatcgc
    12541 cgtctcagga ggctgtaagc agctgcttaa taaggcagta agtcgtcagc tggaggagaa
    12601 gatcaataac agagagactg tgatcgccac aaaaggagac cgtaactacg tcaatattgc
    12661 ggaagacatt gccgtggact atgcctggca gctgatcacg ccgatcatct gcgaagggga
    12721 catcatcgga agtgtgatcc tgttggaaaa tgacgggaag agcaaaatgg gagaagtgga
    12781 gcagaaactg gctctgtctg cggcaggctt cctgggcaga cagatggaac aataaataat
    12841 tactttgttt atttacaatt tggaatagaa aagtgcaacc ggttgcaaaa atcttttcca
    12901 aaaatgaagc aagtatattg attttcagtt aaattatcca tataatggaa tagaaaagtg
    12961 caaccagttg caaaaatcta ttccagggag aaaatatatg gatattcgca gagatttcta
    13021 tttggaaaag ttaataaaaa gaaagagcaa tggattaatc aaggtgatta ccggtattcg
    13081 aagatgtggt aagtcttatc tgctgaataa cattttctat catcatttgt tagattccgg
    13141 tgtggaagca gaccatatca tacggtttgc ctttgattct gcggatgatc tttacctgat
    13201 cggtgaaagt cttattcaga tcgaaaagga aaagcggggt gtggatccgg agaaatttat
    13261 ggcatatatt cggtcgaaaa ctacaggaga gggaatatat tatctgctgt tggatgaaat
    13321 tcagatgctg gattgttttg aggctgtttt gaatggatac cttcgcaaag agaatattga
    13381 tgtgtttgtg acgggaagta acgcaaaact gttgtcaaag gatattgcta cggaatttgc
    13441 aggcaggggt gatgaaattc atatgtaccc tctcagcttt gctgaattta tgtctgttta
    13501 taacggagat aaatataccg ggttatctga atatatgctt tacggtggaa ttccattagt
    13561 ggttctgcgg gatggtgccg gggataaagc aacagcgctg cagactttat ttaacgaaat
    13621 ttatatccgt gatattacga aacggaatcg tataaagaat attggggaat tggaagatct
    13681 gctaaatatt ctttcctctg cgatcggttc gttgaccaat ccggaaaaac taaagaacac
    13741 ttttaaaacc gtgaagaaat ctaaaattac ttctgcaacc ataaagaaat atttggatta
    13801 ctttgaagat tcttttttga tagagtcagc acagcgttat gatattaagg gaaagtcata
    13861 tatagagact cctaagaaat attatttttc tgatttgggg cttcggaatg ccagaattca
    13921 tttccggcag ttggagcaga cacattccat ggaaaatgta atctataacg aactccggat
    13981 gcggggatat agtgtggatg taggtgttgt ccctatcgca gaaaaagatt tggaaggaaa
    14041 ggtaaaccgc aggcagctgg aagtggattt tgtctgcaat cttggttctg ccagatatta
    14101 cattcaatcg gcatattctc ttccggatga agcaaagcgg acgcaggaaa tccgaccgtt
    14161 ccggaagatt gatgactctt ttaagaaact tgtcattaca aaagatattg tgcagccata
    14221 ttatgatgaa tatggtattc tgacaatcaa tatttatgat ttcctgttga atccggaatg
    14281 cctgcaaggt taaagataac aagatcagaa acaaataaaa aacggtcgta accgggttaa
    14341 gataccacct ggttacgacc gtttgtctta ccgatatata atttctcatc agcttctttt
    14401 aatattgtat gaagatcttt gtcggactca caaaccagtc cgaaggtcat agttactttt
    14461 acgtgctgcc cctcatagag cgtatccagt tcgtggattt tgtccatgag aagctccagc
    14521 tgcttccggg cttctgtaag atctacattt tcaaatacca gtagaaattc ctcaccaccc
    14581 caacgcgcgg caaagccacg gcgccacata tgctgcttca gcagggcaga aacatttttc
    14641 agtacggcat ccccgcattc atgaccgtag gtatcgttaa cctttttgaa aaagtcaata
    14701 tctccgatgg cgagtgcaaa agaatggcgg gaggatctgg ctttctcgaa aatctgccgc
    14761 agctttttgt ctccgctgcg gcggttgtac agctcggtca gggcatcctg ctcgatcatg
    14821 gtacgaagag aacgctgcat ggacagggca cagcgaccga tatcacctaa ctcatcactg
    14881 cgctttgtta cggaatgatc cagtgtggcg ttcagattcc cggaggctac ttccgacagg
    14941 aaactatgga tatggagtaa tacagccacg aatcttctgg tgtaaaaata gatgcagaca
    15001 gatacgagag ccacaaaacc gatgatcagc cacgtcagcg gatagacgta attctgaatg
    15061 gatagggaga ctgtttcact gggtctgccc acaaaaagca ttccgacgac gctgccgtcc
    15121 tgattgcgca gaggtatata gtaggaaaaa taagctttgc cgttaatgag agtatgagtg
    15181 tagaaatggt tttcgccggt gttcaaaaca tcccggatca caatatcggg agcaccggaa
    15241 ccgacgatgc gttcgccctg ggcattatac agggtcgtca gaatgcgggt atcctgatag
    15301 aacagggtga tgtccagtcc ggtatcctct ttgaactgat ccagaagaga atagtccctg
    15361 gtgatatccg tctctccttt atacagaaga taagcaacat ctccctccag gtgataatcg
    15421 ccgggatagg cagcgttcag aagtgtagtg gcacttttcg tggcatcata caattcacgt
    15481 tccacttcct gatacatagt ttttgtgaac cattgggaag tgaaaaacaa catcgcaatt
    15541 cccagcgcca gtaaaggaag cagtccgatg atctgcagag tatatgatat tttaccgctg
    15601 ctttttttct gtgtattttc catagaattc cctgcccctt ccgataagat cggtacacat
    15661 tagagtataa aaatattata tgaaataaat gtcggaaaag caagaaataa aagctttata
    15721 agcaaatctc aacatccggc ttgacaacgc aaaaaaatga gatatataat agcggtatat
    15781 caaaaatgca ttgacgaaga gagtaacttt tccgcagaaa ggcaaagcga accggtgatg
    15841 gtgagagatc ggtgcgagta agggaatcgt gaaaatcact tctgagctgt cagaacaaaa
    15901 gcccaggtca gtagttctga acggaatccc gccgttaccg ggaagcatat cactcctgtg
    15961 agattacaca tgtggaatca tatgggaagt atgttgtaag aggtggttaa cgaatgcatg
    16021 agcttcgtcc tgttacggga cggggctttt tttgatacta ttcaggaaag catcgaagat
    16081 gcgattttgt gaatgaggcg ctgaatagta tgatgctgta ataagaaagg aaaggaagca
    16141 cagtatgtac aagcaggtac ccacaagcct gaattttgta gaccgcgaga aagcagtcga
    16201 gaaattctgg gaagacaacg acattttcaa aaaaagtatg gagcatcgta aggaaggcga
    16261 aacctatacc ttctacgacg gaccgcctac cgctaacggt aagcctcaca tcggtcatgt
    16321 agagacccgt accatcaagg atatgatccc cagataccgt accatgaagg gatatatggt
    16381 tccccgtaaa gccggctggg atactcacgg acttcccgta gaactggaag tcgagaagat
    16441 gctgggtctg gacggcaagg agcagatcga ggaatacggt ctggcacctt tcattgacaa
    16501 atgtaaagag agcgtctgga aatataaggg aatgtgggaa gatttctccc gtaccgttgg
    16561 tttctgggcg gacatggata acccttatgt tacctatgat gacaatttca tcgagtccga
    16621 gtggtgggca ctgaaaacca tctgggacaa gggtctgttg tacaagggct tcaagattgt
    16681 tccctactgc cctcgctgcg gaaccccgct gtccagtcac gaagtggcac agggctataa
    16741 ggcagtcaaa gagcgctccg ctatcgtacg tttcaaggta aagggcgagg atgcatattt
    16801 cctggcatgg accaccactc cctggaccct tccttccaac gttgcactgt gcgtgaatcc
    16861 ggaggagacc tacctgaagg tgaaggctgc tgacggttat acctattata ttgcaaaggc
    16921 actggcagac aaggttctgg gacgtcttgc agaagaaggc aaggatgctt acgaagtact
    16981 ggagacttat gttggtaaag atctggagta caaggagtac gagcctctgt acaagtgtgc
    17041 gggagatgcc gctgaaaagc agaagaaaaa ggcacatttc gtaacctgcg acggttatgt
    17101 aaccatgacc gacggtactg gtatcgttca tattgcaccg gctttcggtg aagacgacag
    17161 ccgtatcgga cgtaattacg agctgccctt cgtacagttc gtagacggca agggtgatct
    17221 gaccgcagag actccttatg ccggtaaatt tgtaaaggat gcagatcctc tggtattaaa
    17281 ggatctggac gcagagggca agctgttcga tgcaccgaag ttcgagcatg attatccttt
    17341 ctgctggaga tgtgatactc cgctgatcta ctatgcaaga gagtcctggt tcatcaagat
    17401 gaccgctgtg aaggatgatc tggtacgtaa taataagacc atcaactgga tccccgcttc
    17461 catcggtgag ggacgtttcg gcaactggct ggagaatatt caggactggg gcgtatcccg
    17521 taaccgttac tggggtactc cgttaaatat ctgggagtgc gaatgcggac accagcattc
    17581 catcggcagc cgtgaagagc tgtacaagat gagcggcaat gagaaggcga agactgtaga
    17641 attccatcgt ccttacattg acgagatcac tatcacctgc cccgagtgcg gcaagcagat
    17701 gaagcgtgtt cccgaggtga tcgactgctg gttcgattcc ggagcaatgc ctttcgcaca
    17761 gcatcattac ccgtttgaga ataaggagct gttcgagcag cagttccctg ccaactttat
    17821 ctccgaggca gtggaccaga cccgtggatg gttctattca ctgttggcag agtccaccct
    17881 gctcttcaac aaggctcctt acaagaacgt tatcgttatg ggccacgtac aggatgagaa
    17941 cggccagaag atgagtaaga gtaagggcaa tgcggtagat cctttcaacg cattggagac
    18001 ctacggtgca gatgccatcc gttggtattt ctacaccagt tccgcaccct ggctgccgaa
    18061 gagattctcc ggcaaggccg tacaggaagg acagcgtaag ttcatgggta ctctgtggaa
    18121 cacctacgca ttcttcgtac tgtatgcgaa tattgataat tttgacgctt ccaaatatac
    18181 actggaatac gacaaactgc ccgtaatgga taagtggctg ctgtccaagc tgaactctac
    18241 cgttgcagaa gtagacagca atctggacca gtaccgtatc cccgaggctg ccaaggctct
    18301 gcaggacttc gtcgatgaga tgagtaactg gtatgtaaga cgcagccgtg agcgtttctg
    18361 ggcaaagggc atggagcagg ataagatcaa tgcctacatg acgctgtaca ccgcactggt
    18421 gaccatctgt aaggcagccg cacccatgat ccccttcatg accgaggata tttaccagaa
    18481 tctggtaaga agcaatgatg catccgctcc cgagagtatc catctgtgtg acttccctgt
    18541 agtcaacaag gatcacatcg acaagaagct ggaagaggat atggaggatg tactggatgc
    18601 tgtcgttatg ggacgtgcgt gccgtaacga ggctgccatc aagaaccgtc agcccatcag
    18661 cagaatgtat atcaaggcag atttcacctt aagtgagttc taccaggaga tcattgagga
    18721 tgagttaaac gtcaaggaag tggtattcac cgatgacgta cgtgacttta cttcctatac
    18781 cttcaagcct cagctgcgta ccgtaggacc taaatatggc aaacagttag gcggcatcca
    18841 gaagcacctg gctgcactgg acggcaacgc agccatggat gaactgaatg cagacggcgc
    18901 attaaagttc gatgtagacg gtgtggctgt ggaactgacc aaggacgatc tgctgatcga
    18961 catggcacag aaggaaggct atgtatctca ggaggacaat aagatgaccg ttgtgctgga
    19021 taccaacctg acaccggaac tggtagagga aggcttcgta tacgagatca tcagcaagat
    19081 ccagaccatg cgtaaggaga gcggcttcga agtgactgat cacatccgcg tatccatcaa
    19141 cggcaacgac aggctgtccg agatcgcccg gaagaacaaa gacgccatca gcggaaaggt
    19201 tctggcagac gaactgacaa gcggtgcgga atatgccgta tccaaggaat ggaacatcaa
    19261 tggcgagaac gcagtgatcg ctgtggagag agtataaaag ttctgcaata aaatgccgga
    19321 ataaagagat tctgtggttt tcccaacggg gaggaccgcg gaatcttttt tgtttcgcaa
    19381 aaacatttac gaaacttacg aaaaccggcg aaaacagttg gaatgcgcat ggaagtttgt
    19441 tataatatta cctgtatttg gttaagaata tggatgaaat cataacgcta tccggtgcgg
    19501 aatatcttga gaagagtaca gtaccggaga ggcaatggag aggagaacac aggtgaaaaa
    19561 atcagtagca gccagcgaga agccggtaaa ggcaaaagag ttcgacaagg agctcttcaa
    19621 gagaagtgtg gaatataatg tgcgcaccct gtatcgtaag aatctggaag aagcagatgc
    19681 acagcagatc ttccaggcag tatcttatgc gatcaaggat cgtatcgtag agaactggat
    19741 ggagacccag aaggcatatg agaaggaaga tcccaagatg gtctactaca tgtccatgga
    19801 gttcctgatg ggaagagcac tgggtaataa cctgatcaac ttaaaggcat ataagcccgt
    19861 agcagaagct ttggaagaac tgggactgga tctgaacctg atcgaagacc aggagccgga
    19921 tgcagcactg ggtaacggtg gtcttggacg tctggcagca tgtttcctgg attccctggc
    19981 aacgctggga tatcccgcat acggctgcgg tatccgttat cgttatggca tgttcaagca
    20041 ggaaatccgc gacggttatc aggtagaggt tcccgataac tggctgatga acggcaatcc
    20101 ttttgaattg cgcagacccg aatacgccaa gatcgtaaag ttcggcggtt atgtcagcgt
    20161 acacaccgat gagaatggca gaaatgtatt tacccaggaa ggttatcagt ctgttaaggc
    20221 aattccgttt gatttcccca tcgtgggtta tggcaacggt attgtaaaca ccctgcgtat
    20281 ctgggatgcg gagcctgtag agtgcttcca gctggattcc tttgacaagg gtgactacca
    20341 gaaggcggta gagcaggaga acctggcacg taatatcgta gaggttctgt atcccaacga
    20401 caatcactat gcaggtaagg agctgcgtct gaagcagcag tatttcttca tttccgcctc
    20461 cgtacaggaa gctgtagaga agtacatgag aaagcatgac gatatccata aattctatga
    20521 gaaggttacg ttccagctga acgataccca tcctactgta gcaatcgcag agctgatgcg
    20581 tgtgttgatg gatgattatt atctgacctg ggaagaagca tgggagatca ccaccaagac
    20641 ctgcgcttac accaaccata ccattatggc ggaagcattg gagaaatggc ccatcgaact
    20701 gttctccaga ctgcttccca gaatctacca gatcgtagag gagatcaacc gcagattcgt
    20761 tctggatatc cagcagaagt acagcaatgt acccggcgtg gatgtacagg agaagatccg
    20821 caagatggcc atcatctatg atggacaggt gaagatggca aatatggcta tcgtttccgg
    20881 ttactcagtc aacggcgtgg caagactgca taccgagatc ctggagaagc aggaactgaa
    20941 ggacttctac gagatgttcc cggagcgctt caacaataag accaacggta tcacccagag
    21001 acgtttcctg ctgcatgcga acccgctgtt agctgactgg gtaacggctc acgtaggtga
    21061 cgactggatc accgatctgc cgcagatctc ccgcctgaag gtatatgcgg atgacaaaaa
    21121 ggcacagcag gaattcatga acattaagta ccagaataaa gtacgtctgg caaaatacat
    21181 tttagagcac aacggtatcg aagtagatcc cagatccatc ttcgatgtac aggtaaagcg
    21241 tctgcatgag tacaagagac agctgctcaa tattctgcat gtaatgcacc tgtataacga
    21301 actgaaggag catccggagc tggatttcta ccccagaacc tttatcttcg gcgccaaggc
    21361 agcagccggt taccgcaatg caaagctgac catcaagctg atcaactccg tagcggatgt
    21421 ggtgaacaac gatcccgcca tcaatggcaa gatcaaggtt gtatttatcg agaactataa
    21481 cgtatccaac gcagagatca tctttgcggc agcagatgtc tccgagcaga tttctaccgc
    21541 aagtaaggaa gcatccggta ccggtaacat gaagttcatg ctgaacggtg ccctgactct
    21601 gggaaccatg gacggtgcca acgtggagat cgtagaagaa gtgggcgagg agaatgcctt
    21661 catcttcggt ctgtccgcac aggaagttat caattatgag aacaacggcg gatatgatcc
    21721 tatggagata ttcaacaacg atcaggatgt ccgcaaggta ctgatgcagc tgatcaatgg
    21781 aaccttcgca ccgggagatc ccgagctgtt ccgtccgctg tacaactctc tgctgaatac
    21841 ccagtgcacc gccaaggcag acacctactt catcctcaag gacttcaagt cctatgcaga
    21901 ggcacagaag agagtggaag ccgcttaccg cgacgaagac aactgggcga agagcgccat
    21961 cctcaatgtg gcttgctctg gcaagtttac ctccgacaga accatccagc agtatgtgga
    22021 tgagatctgg catctggata aagtggtact gaagtaaatg gaaagttagt aaagaaatgc
    22081 tatcaaatcc ccgtagccct tggcagggta taagaaacgc ggcacgctcg cgctctgtcg
    22141 cagagcgtag cggtacataa gcggtcacaa tacgaccgct aagtaccgac gtctgcttac
    22201 ggcactgctt attcttatac ccagc
//
LOCUS       DAWDHD010000026        21922 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_171_length_21922_cov_11.123245, whole genome shotgun sequence.
ACCESSION   DAWDHD010000026 DAWDHD010000000
VERSION     DAWDHD010000026.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 21922)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 21922)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..21922
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_171_length_21922_cov_11.123245"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         4967 a         6118 c         4626 g         6211 t
ORIGIN      
        1 ttaccggtct gtctacttgc ggtgcggatc ttccggtctg gggtgctgca ggtctggttc
       61 cctgcggtgc cggtcttccg gcctggggtg ccgtaggtct ggctccctgt gtcgcgggtc
      121 ttgcctgctg gcctgccggt ctctgtccct gcaccggtct gccgccctgt ccggcgggct
      181 ttcctgcggg acgtctctct gctcccactt catgcatcac tttctggcca ccctgggttc
      241 tgccgccctg aggtgccggt cttccaccct gacctctgcc ggtctggggt ctgtccactc
      301 ttcttctgcg aagtgctgct tcctccgcat cagagaaatc agccgcatcc gtcatatcct
      361 tgatcttgaa gatcagcaga gtgactgctg ccgccgctgc caccagaaga atgacaagaa
      421 taatgatcac gaccttctga ctggtgatct tcttctgatt ggtctcaaac tgcgcattga
      481 tctcagtaat ctggtcaata gagctgaaca gatcatcgat cttgtattgt ttctgtccta
      541 ccatatctaa cagataccat gcatctccct gcaactgggt atcccagtcc ttggaagtgg
      601 tctgtgtatc ttcggcgggc tgttcttccg aaggctgttc ttccgcaggc tgctctgtgg
      661 aaggtgcctg cagttctccg gataacgcca cataatcgga acggataaat ccggtcactt
      721 cggtattgtt ggcgatgaaa ttcacctgat accaggtatt gccgtccgta ccggtagcct
      781 gtccggtgac ggtcagagcc attccgttct ctgctgtggt tacgatctgg ctcgtggtag
      841 atgcattgct gcgcacacgc accgactgtc cgcccgtcac ggtagcactt acaggctcca
      901 ccgctgtgac atccaccggt gtttcattca ctgccggagc cggtgttgct gcggtagtcg
      961 tcgttgtagt tgtcgtggtg gtcgtagttg aagaagacgg cggggtagat ccgtcggtaa
     1021 tgcttaccag atcggaacga atatatccca gcgtattcgc atccacatat acctcatacc
     1081 aggtatagcc atccgctccc ttggtctggc tcttgatgga aatggtctta tcctttgccg
     1141 cactgccgat gacttcactg gaagaatccg cactcttacg gatctttgca ctggatgctg
     1201 ttaccttggc tgcgctttcc gcatggctga caaacgccag attctcggca aaaagtcctg
     1261 ctcccagcac aagcaccaat gcaagcattc cccagaaagc cttgaattta cttgttctct
     1321 gtactgtctt tttcatctgc aactccttct gtatcctgac atgtcttacg cttcatcgat
     1381 tgccttgcca atgtcatgac gcatgtattt gttctcgaag gtgatccatt ccgttgccgc
     1441 ataggcattg gcacgggcct ccttcagggt agctccctta gctgtcactc ccagaacacg
     1501 tccgccgttt gtcacgattc ccttgtctgt gagcttcgtt cccgcatgga aacaataata
     1561 gccgtctttt ccgttgaact tctccagtcc gtttatcaca taacccttct cataggatac
     1621 cggatagcca tcactggcca gaaccacaca gactgcagca ttgtcttcaa actgcagatc
     1681 gatctgatcc agagtaccat ccacacaagc ctccatgacc tcgataatat cgttcttcat
     1741 gcggggcagt accacctgtg cctccgggtc accgaatctt gcattgtact ccagtacttt
     1801 ggggccgttc ggtgtcagca tcagtccgaa gaaaataacg cctttgaaag gtctgccttc
     1861 cgctgccatg gcatccaccg tagcctggta aatatatttc ttgcagaatg catccacttc
     1921 ttcggtatag aacgggctgg gagaaaaggt tcccataccg ccggtattca gtccctgatc
     1981 gccgtccttc gctctcttgt gatcctgtgc agaagtcatg atccggatgg tcttgccatc
     2041 tacaaaggaa agtacactga cttctcttcc ggtcataaac tcttcgatga ccatacgatt
     2101 accagccgca ccgaatttct tgtccagcat gatctcatcc acgcctgcca gagcttcctc
     2161 caacgtattg cagatcagta cgccttttcc cagtgccagg ccgtctgcct tcagcacgat
     2221 agggaaactt gcctgctcct tcagatattt gatggcatcc tccgcattat cgaaattctc
     2281 ataggctgcg gtaggaatat tatatttttt cataagatcc ttggaaaaag ccttgctgcc
     2341 ctccaggatt gctgcatttt tacggggtcc gaaagcacgc aggccctctg cctccagtgc
     2401 atctaccagt ccgcctacca gaggatcgtc catacctacg atggtcaggt caacggcctg
     2461 ttccttcgca aaagcaacga tcttgtcaaa ttccatggct ccgatgggaa cacattctgc
     2521 gatctgaccg attccggcat tgcccggagc acaatagatc ttatctacct taggactctt
     2581 tgcaacacag gttgcaatgg catgctctct gccaccgcca cctacgatca atactttcat
     2641 acccatatta tgtttctcct tattccgtaa ctattcaccc cccattcgca aaaccgcctc
     2701 tgcgaggctt tcctgaataa catgtttgta tgctatttgc tcatggtgat tacttcgccc
     2761 attggctctg aatagttact catttgttat gctttacttt gccgtctacg acgcagatcc
     2821 gtccgttggc aatgtcatcc accgcctgag gcagaagcag ccactcagcc tgttccatga
     2881 cacgtctctg taagatttcc ggggtatcat catcttccac tgccactgct ttctgtgcaa
     2941 tgatagggcc ttcatccatg ccttcattca caaaatgcac ggtggctccg gtgaccttca
     3001 caccgcgctc cagtgccttt tcatgcacat gaagtccata gtatcccaca ccacagaaag
     3061 aggggatcag agacggatga atattgatga tacgattact gtattcatgc accatagcct
     3121 ccgggatctt caccagaaag cctgccagca cgatcagatc cggggtaaat tccttcacct
     3181 ttgcaagcaa cgcatcgtta aatacttctc tgctctcata atccttcgga gacacacata
     3241 ccgcaggaat tcctgcatta cgtgctctct ccaggctctt caccgtcttg ttgttgctga
     3301 tgacaccgac gatctgcgca ttggtgacct tgcccgctgc ggtagcatca atgatcgcct
     3361 gcagattcgt tccgccaccg gataccagta cgactactct taacataggg tcacaccctt
     3421 ttcaccggct tcgcaggtac ctaccacgta agcggtctcc cctgctgcct tcagtgcttc
     3481 cagagtcttg tccacatctg cgggatccag tgccagcacc atgccaagac ccatgttgta
     3541 ggtgttgtac atcatctcgt cagcaatgtt accctttttc tgcatcagac ggaaaatagc
     3601 gggtacttcg tagctgtcct tcttcacaac tgcgcggatg ccgtcaggaa gcattctggg
     3661 aatattctca tagaaaccgc cgccggtaat atggctgcag ccctttacct taacgcctgc
     3721 gttcttcaca gacttcatag ccttcacata gattctggtg ggagcaagca gtgcttcacc
     3781 cagagtcttg cccagttcgt cataataggt atccaggctc tctttggtca tatcgaatac
     3841 ctttctcacc agggagaaac cgttggaatg tacaccggag gaagcgattc ccaccagtac
     3901 atcaccgggc ttgatgttct cgccggtgat cagatcctta cgctctacca cacctactgc
     3961 aaatccagcc agatcgtact catcctcagg catcagtccg ggatgctctg cggtctcacc
     4021 gcctaccagt gctgcgtcag actgcttaca gccctctgcc acgcccttta cgatggtagc
     4081 gatcttctcg ggaatattgc gtccacatgc gatataatcc aggaaaaata aaggttcgcc
     4141 acctgcacat gccacatcat ttacacacat ggccacacag tcgataccga tggtatcatg
     4201 cttgtccatt aaaaatgcca gcttcagctt ggtgcctaca ccatcggttc cggacaggag
     4261 cacgggatcc tccatatctt tgatggcact cagggaaaat gctccggaga aaccaccgat
     4321 accgcccatt acttcgggac gcatggtctc cttcacatac ttcttcatca gttctacaga
     4381 cttgtagcct gcttcgatgt ctactccagc tttcttgtaa tccattttct cctcatttcc
     4441 gccgcagggg ctctttcgtt ttatttggtg gggcagcctc ttaagaggtc ccggattctc
     4501 tgtcttatct tcttattttt tcatgccttc cagatatgct ttatagccga tctcctgcag
     4561 ttttgcatct ttggcttcta cctgttcttt taatttggtg ctgtatgctt ttaactttcc
     4621 gagaagttct gcgtcggaag tagccaggat cttggctgcc agaagtcctg cattggcgcc
     4681 gccgttaatg gctactgtgg caaccgggat gccggagggc atctgaacga tggaatacag
     4741 agaatctctg cctcccaggg aagtggtgtg catcgggatt ccgatgacgg gcatagggaa
     4801 aatagcggca cacatgccgg gcagatgcgc tgccataccg gctccggcaa tgatcacctt
     4861 gaagcctttt tcctccgcac tttttgcata ctcaaaaaac acatccggct ctctgtgagc
     4921 gctgatcacg gtcatctcat aagggatgcc cagttcttcc agaatgtctg cggctttcgc
     4981 catcacaggc atatcggaat cactacccat tacaatgcca accttagggc aattctctgt
     5041 atttgccata atgtcctcct catgtatcgg ttgcaccgat tttatccata tatattttat
     5101 cttactaaaa tcttttcaat gatgcaatgg ttttatgaaa tcttatgata actattcaga
     5161 gccatgcaaa attgctttct gcaaatgctt gctgaatagt tttaatccaa ctcccgcagc
     5221 ggctcattca gttcttccgt tccgggaagg acaaatctgc cattcgcaat aatggggatc
     5281 cgcccgccgt cctttttcac tgcggtcagc tctgccagtt catcgtaagg aatggtaatg
     5341 tccgtatggc agttaaaata agccttggaa gggttcacac tgcgaagggc ggccacctca
     5401 ttgtctctgg ccacgatctc tttaccgtcg ggattgtata ccttcacctc ttccgcatgg
     5461 gaatagcagg tatcgcccac tgcaaaatgc ggtcccatct tctccgcgat caggatcggc
     5521 atcctggctt ccacgcccag acgctttgcc gccacatatg ctgtagtgtt tgtgccgatg
     5581 gcaaattctc ccagcggcag tgtcttatgg cggaacagga tattttcctt aatgaatgcc
     5641 ttgttctctt cctccgtggc aaaattagca cagttgtagt ttttaatcct gccgttttcg
     5701 aaggtgatct ccagattctt atattccaag ccgttcagga acacttttgt cacatgcagc
     5761 acaccgtttg tgccttccag caccggagag gtaaatactt ctcccacggg aatattcacg
     5821 tctgccacgc agttttcaaa aatagtttct ttggcaggat ctttgagctt ccacagattc
     5881 actgtgatat ccgtgtgatt accgttacag cccttcacct cacagtggtc tgcctgatcc
     5941 agcgcatcga tgatggtctg ctggatcctc tgatattttt tataatccag ggtgttgatc
     6001 cgaatagtct cccggaaaag ttcttcaaag cagtccccta cctccgggat ggggaacgca
     6061 ataatcgtaa agctgcgctc ttcctcaata atatactgtc tctgcaactg tcccatctgg
     6121 gtgcggtgct ccaccataag agtgctctgt tcatccgtca ttttcaccgc ttccggcttg
     6181 ttcaccggtg cgaaatcttt ctcaccgaag gtctccacca cggccgggcc cgcatagcct
     6241 ctggcctctg tcttatattt ttcaaaagcg gtatgcatga cttccagttt ccggctgcaa
     6301 tacattttat ccaggaacag tgctttgtca tccttgtgat cgaactcata ctgtctgtta
     6361 gggctgacac tgtagaagcc gtttttataa atggagggat tatataaaat actggcagct
     6421 gcacggtaga tcacaggctg caatcccatt atgtcaaaat tctccagagc tctccgcatc
     6481 atgcgttcga agcccaactg ataacggaca tccaccgcag cttttttcga cagatccttg
     6541 ccggtcactt caaaaccgat gcggtacccc tcggtataag tgtctgccat ggtatttatg
     6601 gtctcttccg gcagggaatt cagaaatttc gccattgcca gttcattttc ccccacgtat
     6661 tctccatagg catagagata acgcagatcg gtcagttccg cccttcggat aatgtctgcc
     6721 gcaaaattat tccccgggca taccagttcc cgtacccgtg cttccgccgc cacatccgcg
     6781 taatcgctga cgaaccagta aaggatctca cggatggacg cataagacgg aagtctgtga
     6841 ttctcctgct cctcatatac aaatgccgca tatacctcag aaaacagttc catacggatc
     6901 accagttcgt ccagtcttcc ctcataggta aaaccgatca ggctccgcat ttccgtataa
     6961 aggaaggcgg ccagtgcgcc gaattcttct cccagacact ttacagctac cgcaggatta
     7021 gcaaagctgt tctcataatt ttccggtaaa atatcttcat acagggcatg gttacgctgt
     7081 ttcagctctt ccaaagtggc agtgttctgt tttccctgct ccaggaactg tctggtatcc
     7141 tcgatcagca gtaaaaattt tgctaccgct gtaaaatatt ctttccagtg ctgcaggctt
     7201 tcttcctgcc agtcctcccc ggggatctcc cggatccggt ctaaggccag ttccagacgt
     7261 tccaataata attctctttc ttccatagat ctgattgctc cttctatcta aataactgct
     7321 gtgtgatcca atgcttctgt tccagaatat ccgttacaga cggcttccca gatacagcaa
     7381 aaagctgaca cctccaaggg caacaacatt taatattgtt cccagcacgg gaaagatacg
     7441 atagatatct ttttcccgaa gggtcagtat tcccaggatc agtccgatca gggaatagat
     7501 ggtggccaac acaccggtaa caccgtatcc accgtgcatc acgccgccct gactgtagga
     7561 cagatacgtc acgatcccca gggacaccag actgatgccc cccaggatgg tcgacatgat
     7621 cgccctgttg gaatgttttt tattcgtaaa aatgtagttc tttttccgca tactattttc
     7681 aagcctttca gtaacctgca ctcccccatg gaacccgaaa agggcacttc cggtatggaa
     7741 atgcccaatt ttcacgccct gacgacgttt tccttctcat ttgtgatcag aacagaatac
     7801 gtgatttgcc ggagaccagc ttgccgccac tgatgatcag caacacacct gccgcgattc
     7861 cgactaccag ggaaatgact cccagtacaa tattcagagc acctgctcca cgcatgatct
     7921 tatagatttt ctcttccatg gaatcctcct gtcttctatc caaaactttc gaagccgtct
     7981 gcttaagcac cgtatacagc ataatattcg tcgattgcct tcttcagagt atcatcaatg
     8041 tatacctgtc ccttgtaagg cttgttgtac agatgatcga tgacatcctg catggttacg
     8101 atggcatgtg catcaaatcc gtacttttcc ttgatctctt ccagtgcgct gaccttgccg
     8161 cccagaccct tttccatacg gttcagggat accatcagac caacgatttc tacattggct
     8221 gctcctctta ccttgggaac ggtctcttcc atagatttgc cggaagtggt aacatcctcg
     8281 atcatgatga ctctgtcgcc atcttttaag gagcttccca ggaagcttcc cttatctgcg
     8341 ccatgatcct tctcttcctt gcggtcggaa cagtaacgaa cctctttgcc gtataatttg
     8401 ctgtaagcca ctgcagttac aacactgatg ggaatgccct tataggcagg tccgaacaat
     8461 acatcaaaat cgtcaccata ggtatcgtga attgctttcg catagctctc gcccagcttc
     8521 atcagctgag aaccggtgac atacgcacct gcgttcatga agaaaggaga ctttctgcca
     8581 ctctttaagg taaactcgcc aaacttcaac gcctgacaat ccaccatgaa ttcaataaat
     8641 tcctgcttgt actgttccat tgtatctaaa aacctccgtg attctcgtat ctttgtcagt
     8701 tctgcccttt atcataccat actttttttt aacattcaat gagtttcctt gggaagaaaa
     8761 aaggcagtcc atcgactgcc taataggtat ctccctgtaa aggtcggata ttcacttcct
     8821 ccagtactgc ctgtggggac agacggaagg tcagggcgat gacttttccg aggtcagagg
     8881 tctctaaaag tgacctgtcc tcttcggagg atttctttct ggtctcggta aaggtgccgc
     8941 cgggattgat gagggatacg ccgatcccca taggcttgca ttcctcccgg agggcatagg
     9001 cataggaacg gagcgcccat ttggaaatgc cgtagatgcc gcccctgtct ctgcggattc
     9061 ccgaagtgga accgatgagt acgatgcggc tgcccggtgc cagcagcgga agaaattcct
     9121 tcaccagacg gtaatatgcc gtcacgttgc tgtcaagcat tgcctgcagt tcctccggtt
     9181 ccggctgcaa tacagatctc tgcgtggaag gatttggaaa tgccgccgca cacaggatca
     9241 gtccgtccac cctgctgccg aattccgtta tgctgccgcc ttttaccgct tccaccagtc
     9301 cggcggtttc ctctgccttc ccggcatcac aggaatacgt tgccgctcct gccagttctt
     9361 ttccggtctc ttccagagtc ttcggatttc tgcccacgat cagcacgtta tttccatcct
     9421 gtaacaattc tctggcggcc gccttgccga tgccgcggct gccaccgatg atcacaatat
     9481 tcatgcgccg ctctccttct tcctagtatc ttccgagaat cgtatccttt acgtcccatc
     9541 tctgaggagg gtaaggggtc tggtcaccgt atcctacatc cacataggtc accaggcgga
     9601 tataatccgg cagattaaat ttcttccgca gtctgtcaca aaattcttct ccggggaaag
     9661 tcagccagac accttccagt cctacggcgt gggctgccag aacgatattc tgtccggcag
     9721 ctccggcatc cagcagctgg tttctcaccg gggtaaagga gttggctctg tagcatctca
     9781 tatcctgtaa gatcaccaga tgcaccggac ctcccggcac gtcactgccg cggaacaatc
     9841 ccggctcgtt tttctcccgt accaccacat agcggatgga ctgtacatta cagccgtgag
     9901 cagcccaaag ccccgcttcc aggatcttat cgatcagttc atccggtact tccttgtcct
     9961 tgaattcccg gacgcttctg cgttccttta caatcgtgaa aaagtctttc tccatctgtt
    10021 ccgtcaccgg cgtaggtttg taaggtgtca gatccacgtc ttcccccttt accagtttct
    10081 ccgccgcatc aatcaggaaa gaggcatacc gatattccgg cagatctttt cccaatcctc
    10141 ttttctccca cagttccagc agatgcttcg tataatctgc ctggttgggt tttaatttct
    10201 ggtggcgata tgcggtggca tatacctgga tctccagggt atgatgggaa cgttctctca
    10261 ctcttgcccg gaattctgtc tcatccatgg ctaaaagctg ctcgtctgtc agacgcatat
    10321 tgtgattcca gatctcaagt acttcttctc tgggtaaata gtccatactg gtgtcacgaa
    10381 acatactgct gcctccttat tctcagtgtt ctccgcctat aagttgcccc aatagttgtt
    10441 gggaatatcc tgtccaccgc tattaatatc tttggcaatt tccgctgcga tctttaacag
    10501 cagaggatca ctgcgtcctc tcttctgctc cagcggaatc tcgtagatct tctggatcgt
    10561 actgggtctt gggcttaaca gcacgactct gtctgccaga aataccgctt cgttgatatc
    10621 atgagtgacg aacaataccg tcttgttctc tgcctgtctg atattgatca gttcctcctg
    10681 cagagtgcat ttggtcagat gatccacggc actgaagggc tcatccatga ggatgacctt
    10741 gggattcatg gcaagggttc tggcgatgcc ggctctctgc cgcataccgc cggacagctg
    10801 tgccggatat ttgttctcaa aaccgttcag gctgaccatg gagatataac gctttaaccg
    10861 ttcttctctc tcttccttgg gtactccggc gatctccagt ccgaatgcca catttttccg
    10921 tacggttctc cagggaaaca gggaggcatc ctggaatacc acgccaatat ctctgctggt
    10981 accgtgtacc ggctgtccat ccagcaatac ttcgccgctt gtagcatcca gcaggcccgc
    11041 cacgatctct aacagggtac tcttaccgca tccgctggga cctacgatgc agacgaattc
    11101 accttctgcc acatcaagat tgatgtcatg cagtgcctgt acttcgtttc catcctgatc
    11161 gatataggtt ttgttgatat ttttaatgga tattgcgggc atttccatta cctccgtttc
    11221 ctgtgtctct tattttcaga gcattctcaa ttattttgct tccgggtcgg tcattcccag
    11281 ggtatctgca gctacctgcg cgaattcgtt ggtgtaggtt ccttcgtagc tgacattttc
    11341 atctacccag ccgtactcct tcagatattc aaaggtggta gccagacgat cttcggggat
    11401 taccggataa tccagccata catcgatttc cgactcgatg gcctttctca taacctcttc
    11461 ggaagtattc atctcttttg ctgcccatgc aacaaattcg tcgaaatagt cattcttaac
    11521 ttcttcatgt acctgataat aagcggtcag gaagcgctgc agcttctctg gctcattttc
    11581 aataaagctg ttgcttgcga tgatcgttcc ggtatagtag tcgggaatgt aatcccatgc
    11641 tctgcccagg atatgtccgg taccgtctgc ctcagccagt gccgcatacg ggttgtggat
    11701 aatggtggca tccacctcac cggaagtcag ggaagcatat gctgtctgat atacaccgtt
    11761 ggcgatcaga tctacattgt ctgtgctgac gccgtttttc tctaacattt tcaatacgga
    11821 aagtctcatt ccgccggaag cacctgcggt acctaccttt ttgccttcca gatcagagaa
    11881 gtccttaata tcgttatttg caaccagcca gtagcatccg gaatatctcc acatatttcc
    11941 taccagcttg gcaggtaccc cattgtagat gccgggaatg taagaactgg gatctccgtc
    12001 agctacatcg atctcgcctc tggtgatcag agacattacg gtagaattgg tatcggcgct
    12061 ggcctctacg ttttcgaact caataccaag ctctttatac agaccctttt caatggcaaa
    12121 acggttcacc gcaccggtca ttccggtagc gaagcaggaa cggatcacga tggtgtctcc
    12181 ggattcctct cctgcggtag ttgcacctgc tgcctggctt ccctcattct gtgcggctcc
    12241 ggttgctgta ttgctgccgg ttgctgcact gctgccgcag cctgccagta ctccggttgc
    12301 cagtgccaca gtcagtaata ccctgaatac tttctttttc ataacgtttt cctcctcgtc
    12361 tattgaaaaa atttatttgc ttatacatgt ctccatcgat agctgatagc atattcgatg
    12421 gcatcgatca gttttaccgc acctacggat aataaggtga tcaggatgat caggaagaac
    12481 atcctcgggg tgttcaacag gttctgggac tcagtcagca gatatccgag tcctgctctc
    12541 gccgactgca tttccgagaa caatacaccg gtcagtgccg ttgccgctgc cagcttcaat
    12601 ccgttgaaga tcgacggcag tgctgccgga ataatgacca gaaatacggt ctggaaacga
    12661 ttggctttaa atacctttgc gaccttcaga tactcctttt tacactcctt ggctccggtc
    12721 actgtgttat acagaattgt aaataccgag aacaaaaata ccagaaacgc cttggactga
    12781 aagccgatgc caaaccacag gatcaacagc ggcaggattg ctactttcgg aactgccatg
    12841 attgccgaac agaatacatt catgtacttc tccgataccg gaaacaatgt ccacaacaga
    12901 cctaccgcaa taccgactac cactgcggac aggtaacctg ccagtgcctc ctggatcgtc
    12961 agcaataagt ggggccataa ggttccgtct gtaatgatct taatgccctc ttccacgatg
    13021 gaagtcgggg acgccacgaa gatcgtaagc accctgccgg aatctactgc ccattgcatc
    13081 cacagaagca gtgccgccac ggtgagcagc tgtgccagat tcacccaggt aaaacgtttc
    13141 ttattcttag ttttcttctt tttttccata agtgtattct cctcgcttca ccttatcgct
    13201 ttcaaagtac tctctcttac aacaggtacc ttccaggatg acattctgtg tgatcctgtg
    13261 ggagagacat ttggaaaagt tctcaatggt agtagtgtcg atcacgtcac agtaaaacgg
    13321 tgcaatctct cggaaccagg gatatttgtc aaaataagtt ctccacactt ccgcataggg
    13381 gcaatgatct tctccccact ccgggatcca tccggtgaag ggaaggtcga taacactcat
    13441 gaaatcttct acggaatctg ccttcaggcc ttgcgccagt gccttttccc tcagctgatc
    13501 cgaccgatcc accgccagct cgaatacggt ctgtctcacg atatctttgg tcttctccag
    13561 tccgaactgg tcatataata ctttgctgaa atgaaagaac tgcatggcga actgtcttgc
    13621 ggcctttctc acttcactta ccaacagttc tctgggaacc gttccttcta ttttataatc
    13681 tgctttactc aatgtccgct tcctccacct tattaaagct gtccataaag gctatgtatt
    13741 cctgcttatc tgcgaatctc ggatgatatt cctgtttggt ccgcttcgtc acttccgcgc
    13801 cgtccttcaa cattcggtaa gcactcaggg caaatatctt ggctgtcagc acataagctt
    13861 cttcctcatc cacaatatcg aaatccacct gatgtaaacc accgacggca ccaccggtat
    13921 tgaaagtgat cacaggaagc agatgctgta catctcccac atctgtggat cctccgctgt
    13981 gttcctccgg tctgacctcg tataccgttt tttctcctgc cagtgctttc accgcatcgg
    14041 ccatctcttc cggtgccggc tgccagagag aaggaagata gcctggcata gtctcgatac
    14101 gataccctgc tcccatggca agtgctcctg ccttgaagga acgatctgtc ttgacggatg
    14161 catctgcgaa agcttccagg gtcttgcctc ttaccagtgt ctccagcacc gcttcgttgg
    14221 gaactacatt gaccagatct ccgcccttgg tcaggatcgg atgaaccctc acgcaatcct
    14281 catcccggaa ggtctcacgg ttcagtgcca gtgcctgtaa ccccaggctt gctgccgata
    14341 aggcgttgac acctttttcc ggacatcccg ccgcatgggc tgcttttcct ttgatacgga
    14401 tcaccttgga tacaaaacca ttgccactgt tacttcccag ccggattccc tctaaggaaa
    14461 tatgatgcgc cagtgccaca tccacatcat cgaaagcacc gatgcggatc agttcacatt
    14521 ttccaccacc gtaagcgatc ttgccatcag cgatcagctg attcttgaat tcgatctcac
    14581 cgtactcttc cgccggtgtg gcgaagaaga tcacctgtcc atccagcttc tgtgccacct
    14641 ctgcatccgt aagtgccagc gccgctccga tgactcctgc cagttgtgcg tgatgtccac
    14701 agcagtgagc tgcctgtgtc tcctgatttg catgtgcatg ctgcggaatc ctcagggcat
    14761 ccagttctcc gatcaatgcc agggatgcat ttccggcctt ttctttattt aaataggctt
    14821 ttgctccggt gatggccaga tctgtctgta ccggcagctg taagctcttc ataaaatcca
    14881 cgaacttttt cgaagtgcgg aattccttgt agcccagctc tgcatgatcg taaatatctc
    14941 ttgcgaactc tacgatggtc tcccggttgt catctatgat cttaaggatt ctctgttctg
    15001 tcgtatcaaa ggttgccgcc acggtgtcat cctcctcgtc ttttatatcc tagtaagtct
    15061 atatgttttc tatgttttag atgatattac cttatatttc ctagtttgtc aatagggtta
    15121 gtatgcattt atacaaaaaa gttaggtaac agttcagata tgttcatggc tgaactgtta
    15181 cctatattta cttacttatt tactctctat tcttcaatac ttccgcaggc accagcttgt
    15241 tcagtctgcc caccagcagg aagttgatca ccagatagca gatcaggatg cctccgtaga
    15301 tcatgccgta caactgcggc gggaactgta aatccattcc gatggctaca ttggaaataa
    15361 aatacgggaa gatcagatcc atggaccatt ttgccaacgg tactcccaac aatgctccca
    15421 acattataat atagaagttc ccgtccagat acagtctgcg gatctctccc tttcggtagc
    15481 cgaatacttt catcagggaa atggagaatg cagaacgatc gatcatgacc ttcatcatca
    15541 gatacatgac gatcatgaag atcagtgcgg agatggccgc cagcatgctg accataggtc
    15601 ccatcatatc cgtaaatatc tgagagcttt cctccacgtt ttctctggat accgtagaat
    15661 acagtctgcc gttgtcgatg tccagtgcat gatcggcgaa tactacatta taatagtcgt
    15721 cttcctgatc gaacagttcc tgcatggcat cccggtctaa aaatacataa acaccggagg
    15781 caaaatgaac gatatctttg acagtaaacg cataatctct ctcgttcact tcatcggaaa
    15841 gcaccagttt gtcaccgacc ttcacaccaa atttctgtgc tgcggcgctg gagatcacga
    15901 tctcattttt cttattgctg gtctccaccg gaaaatatgg attatcctta tcgattccca
    15961 gtaccgtcac atccagatta taaccgtaag cctctttttt cagactctcc acataagccg
    16021 gagtaccatc ctccggcaca tcctccgtgg gatatttgta agtatacata taagcatagg
    16081 tcgtctcatc caggcaggcg tctccaaagt tcttacacag aacatagcag tctgcagaga
    16141 tcaaaagtac cagcaggcag ataaacatac cgatcaccac cgccacggca gaacgcatct
    16201 cccgaaggag ctgacggacc tggaatctgt gaaggaactt catatttccc agatttacat
    16261 cttgtatctt cccggccctg cctgcggtct gttcatttcg aagcagagac agtgccgtgc
    16321 gcttcaactt cttgctgatc accagacagt ttaccaatgc ggccgtcacc ggcggcatga
    16381 ccagactgta gaccagcaga taggccggag tcacgatttc catgggtggc agggaaaaat
    16441 acacgatact gtcctgcatc tgccagccgg taccatactt cgaaaagccc aggacacagc
    16501 cgaccgcacc tcctaagaag gagatcatca tgggattgag cagataatgg aacaaaagct
    16561 gccctcgcgt cacacccaaa gcgtacagcg caccgatgac gctgctctct ttttcaatat
    16621 tatgtaccac aaatacggag atcacgtaag tgaacatgac catcaggata ataccggata
    16681 ccataccggc atatttattg atgatcacat ccccgctggc ggcatcgatt ctgggattat
    16741 cccctgccac cagaaactgt gtcagattgt cgatatcgaa agtaaaatac tcatcgatca
    16801 tatcattggc ttcatcctgc agttcctgca caccgtcccg cagttccctg ctgccatccg
    16861 caatctcatc cacggcatcc gtataatcga tgattccttc ccggaaatcc tccagatccg
    16921 ccaaagtatc cttcgcgtcc ttcaaggaat cccgcagcct ggcatccaca ctgctgccct
    16981 ctgccatcag ctggtccagc tcttccttat aattgtcggc agtcagttct acctgaataa
    17041 cactttcttc cagctgtgcc tccaccatat ccatcagtgc atccaccacg gcatctgtgc
    17101 cgtctaccag aggctcactg gcggtataaa gcacgctcag tccctggaac agctcctggg
    17161 atcctccggc tgcttctccg acaccggcgg tataggcttt caccccatcc cgaaactgtg
    17221 ccacggtgtc cagctgactt ttcactgcag acagctgccc cgctgcctgg gatgccccca
    17281 tctgtgtaaa gacagttgcc agctgatcca gctgctctcc gtaaccgcca gccgtcagtc
    17341 ccggaaagct gatcccgtat gccgcataag gttccaactc tgcctggagc tgttcattca
    17401 ccatgcccag aatggcatta aaaatagcat cagctccctg attcaacgca gcactgtttt
    17461 ccgtgatctg actcataccg cccaccagtg ctccgccgcc ggcagaagct gcattaactc
    17521 cgtccgtgta ggatttcaca ccatcccgga actcctgcag atcttccaga gactttttcg
    17581 cgtcctgcag gttctgccgc agggatgcag tgtaggcatg aggattagcg atcatggcat
    17641 tcagctgttg ttcataattt cctgaagtca gtgtcacttc cactccggca tccttcaggc
    17701 tgtcattcac ctgttccagc atggcatcaa acaactcatc cgccgcatcc gtcagttctg
    17761 tattatgatc cgccagttca tccacaccgt ccaccagctc atccacgcca tccagaagtt
    17821 cctggatacc atcctgaata tcgttcttcg tctcttccgc atctgccagc atttccagaa
    17881 aataagtatc cgtcacctta ctgcggtcca gttcgaagga ttgtaacagt tccttcagtt
    17941 cctgatccgt catggtatca ttcagcagat aggtgtaagt ataatcctcc gtccggaaat
    18001 tctgtccgcc tgctttctga gcttcattat cctctgccgt gacaaatccg aagccaaaca
    18061 gattgctgtc tacagtggta tctgacgttt tctcaaaagc agcatcgtaa tccggcgtgc
    18121 tgccgattcc ggcaacggtg aactcttttc ccccaacgat aaagatatct cctaattgca
    18181 gctcatgttt ttccgcataa tgctgttcca acaggatctc tccctgtgcc ggaatttcct
    18241 ccccttctgc tggaacgaac agatctaccg attctcttgc ctgatagaca cgaaacgtgg
    18301 actgtcccag atgaaaatcc agggaaaaat ccctctgcag tgtcacacct ttttccgtga
    18361 tctgtgcggt ctctttttcg gtcagcggaa caaaaacgcc aaactgtccg tcctctctgt
    18421 gatggacacg tccgctctcc ttcgtcccct gcataatagt ctcggcagct cccacaatag
    18481 ccaccaccat atacatgacc agtgccacca ggaaaaacag tgccagatac cgaagcaggt
    18541 tttcccggat atcccgaagt actcgtttgc ttaatatcct ctgcatccta caaatcctcc
    18601 agttctgccg caggaacacg tttctcgttc cgatagttct tgctgatctg tccgtccttg
    18661 atcataatga cctgatgtac catgttttta atggaattat tatgcgtaac aataagcatc
    18721 gtagtgccat actgtctgtt gatatcctcc agcagtatca ggatgtcccg ggaagtcctg
    18781 gaatccagcg cacccgtagg ctcatcgcat aacagaaggc tcggattctt gattaaggct
    18841 ctggcgatgg cacagcgttg ctgttgtccg ccggaaagtt gtgccgggaa cttgttctga
    18901 tgttccgtca gtcccagtac ctgcagcagt tcctccatat tcaaaggatt cttcgtcaga
    18961 tactggcaaa cctggatatt ttccttcacc gtcagattcg gcaccagatt ataaaactgg
    19021 aaaataaaac ccagcttctc ccgacggtaa ttggaaagtg ctttactgtt aagccccgcc
    19081 acctcctgtc catccactac gatggaaccg gaatccacgg tatccagtcc tccgatgcag
    19141 ttgagcagcg tggatttgcc ggaaccgctg gtgccctgga tgacacacat ttcaccttgt
    19201 tccactcctg cggtgattcc ctttaatacc tgtacataac cgccgccttc tccatagctc
    19261 ttcttcagat ccttcacttc cagatacata tcctgcctcc tcatcactaa tgataatgat
    19321 tttcattaac ataacactgt tatgttaatg ctctgaaaca gcacccctgc aaagtccaaa
    19381 cttccttggg gtgcattgta ttttagcttt ctgtcattat gcccatccat ccgcgcacca
    19441 gatacatcgt caaggcatcc acatgcttca atgccactct ctcttctgtc tcatgcagaa
    19501 acaggtgtac aaaagtatct accataatgt gggccatcca gtgtgtcatg taatcatcga
    19561 cccgcggtct tccggtggca ttcgtcacca tatcgcataa tctgcggtac tgctgctccg
    19621 agatatccac gacctcatcc agacaatttt caaatcggga cccctgagat ttcgtcagga
    19681 gcagcagaaa ggcctcccgg ttcgcataca tctgccggat gatctgtctg gatgcttcca
    19741 gatccccgtc atccgcaata tccagctgtg ccacctgatc ctcgttgtgc atctctgtgg
    19801 cataatgttc ttccattatt gcatacagct gccgcaacgg ctcttccacc agtgctgcca
    19861 acagatcttc cttatccttg aagaaaaaat acagcgcccc tgtggtcact ccggcctctt
    19921 tacagatatt ccgcagggaa gctttcatat atcctttttc cagaaactcc cttttggcac
    19981 attccagaag gtgctgcctg gtttctttat tctcattttg cgtttcctgt tttaagggca
    20041 ttgcgatctc cttcgggaat aatactcctc tcatagaaag catcccctgc ctgcaagcag
    20101 tcaggtatgt tttctatgga agagtttatt ctatcatatg ttcactcgtt cacataacgt
    20161 tgttatgtct gcattataaa taaaaacagg attgctgtca accctgtttt tattttcgtc
    20221 atttcgtgtt attttgtttt cagctgttcg atcttttcct gtaaatctgc aatggctctc
    20281 tggctgttct taatggtctc gcactgcttc tcaaaaggag gctccggcat atcgctgtac
    20341 tgttcaaaat agatcttgcc gatctccaga tagttcttct tcatgacttc ctcgcaggta
    20401 gagatctggc tcttcagatt agcaatggct gccagttctt ttgctttgtc tgccgctact
    20461 tttccctttt cggtgatggt atcaccgatc ttctctataa tatccatcgt atgatctcct
    20521 ttctcttcct accgcccgca ctttggatct gtctgtcctt cccgtccggg ctgtttcggt
    20581 ttccatattc cgtgtgtatt attgtacctt cttccggata ccgcgtcaag tataagcgct
    20641 acttgcgcat ttcaaagaat ctgtgctata atcgccttgt aatactattg gaggtgacat
    20701 catgcagccg ctatatacca ctctgaaagt taataatgag attgaattgt gcgaagtatc
    20761 cgatcccgat tgcaagcagc tcatcgagag agcattgctg aaaaaccgga tttcttatta
    20821 tatccgctgg ccgaaggctt ccctcttcag ccgcaaaaaa gatgcctgca tcatctgcat
    20881 caacgacaat gtcaaggatg ctgcggaaga aatcgtccgc gggatctgtg acgaatccgg
    20941 atacaatgtg cgctttattt taaaacgttc ctctaataac tatttataga cccttaaagc
    21001 acataatttt atgttttttt cgtttattga ttgatttttt ctcactaatg gatcataatg
    21061 atgatatcat ttaatacaaa ggaggggctt tctaatggaa aagcgtgtta acaagcatgt
    21121 atttgcctgg gttttctgct ttttacttgg tgagttgggt gttgatagat tcgtcagagg
    21181 tcaggttggt ctcggaattt tgaagcttct cactgccggt ggctgcggag tatggagcct
    21241 ggtcgactgg atcattgctc ttaccaaggc atacggtgct gcttatgccg gttccgaaga
    21301 agttgtattc gtagacggta aatatacagc ataatcacag gataaaaaaa gcttttgccg
    21361 gagacgacaa aagctttttt ctttttatag gttgccgatc atgaataaaa acataaaaca
    21421 aactatattt cttatttcta ttattgcact actcctgctt ttcctgtggt tctcaggata
    21481 tcaatgccct ttactatttt tttttggcat tccatgccct ttctgtggaa tgaccagagc
    21541 atttttatgc ctattaagcg gtgatcttac aacagccttt tattatcatc ctctctggcc
    21601 tgtagtattg ggaattttta ttatttttcc tctttggaaa ttccggcttc ttcacttatc
    21661 ggacaaatgg atcgatcatg gggcgtttgc gattggaatt ctgttcttgc tctgctttat
    21721 cataaggcat gtaacacatt ctcccattgt acagattgat tttacaaaag gtatttttca
    21781 tttcttttca tatatgaata catgaaaaag gagctgtaat acagcccctt ttttcaatgc
    21841 tcttatgcaa tcgctttatc cgttttctca atcaaaatcg aatttggtgt aacgctcata
    21901 catagcgcca taacggaatc tt
//
LOCUS       DAWDHD010000027        21880 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_172_length_21880_cov_13.893746, whole genome shotgun sequence.
ACCESSION   DAWDHD010000027 DAWDHD010000000
VERSION     DAWDHD010000027.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 21880)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 21880)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..21880
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_172_length_21880_cov_13.893746"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         5823 a         5548 c         4498 g         6011 t
ORIGIN      
        1 ccacttttgt cagggtcact ccctctacac ggtctctgcc gtgaatctgt gccacagtag
       61 tattcagata cagagggatg ttgaaatcgt ccaggcactg tacgatatta cgcttcaggc
      121 cgccactctg ggggcgaagc tctgccacgc actgtacttt ggcaccttcc agcaccatac
      181 gtctcgccat gataagtccg atgtctccgg aaccaaggat caccacacat ttaccgggca
      241 tccgccctct gatatttaca taatactgtg ccgttccggc actgtagatg cctgccggtc
      301 tgctgccggg gatattcagt gctccccggg aacgttctct gcatcccata gccaggatca
      361 cggtctccgt ctcgatttcc agcagcccgt cctctttgtt catggcagtg acttttttac
      421 gtccgtcagg aagcagagaa atattggtta ccatggtgtt cagcagatag gggatctgcg
      481 cttcttctgc catatcgata taacgttccg catactccgg tccggtcagc tcctctttaa
      541 aagtatgcag tccgaaaccg ttgtggatac actggttcag aatgcctccc agttctctgt
      601 cacgttctaa gatcagcact ttttcacagc cggcctttct cgcagacagc gctgctgcca
      661 gtcccgcggg acctcctccg atgatgacga tgtcttctgt gcgtttactc atggagtgcc
      721 tcctttccga caggcgcctg tgtcctcttc catgttccgc cctctccggg tgcggtgtct
      781 ttatttttac cggtcagaat ctctgagccg gttccttcct tagtgatctg tgccggatca
      841 aggtgcagtt ctctcgacaa tatctccagc gtcttcgggg aacagaatcc cgcctgacat
      901 ctgcccatac cggctctggt gcggcgtttg acaccatcca aggtcgtcgc tcccaccgga
      961 cggtgaatgg cttccagaat ttcaccttcc gtcaccagtt cacaacggca gatcacattg
     1021 gcaaacgcgg gattttcccg gatcaggact tctctctcct cttccgtggc aagtgccatg
     1081 ctgggaatgc cttttctggt atctataaaa tccgacttac gttctgcggg ataaatattt
     1141 tcaacaatct gtgccacata ctcaccgatc gccggtgcac tggtcagtcc cggagattca
     1201 atacccgcca catcgatgaa cccaggatgc cctgctgcct cttcgatgag gaaatccgat
     1261 ttttccgcag gatcttcgtg taccagatga gcacgcagtc cggcaaaaga ggtgatggtc
     1321 tgtctgccgt ccagcgcgtc cacactgtta cttcccagat tcagaacctt cgtcagtcct
     1381 tctgctgtcg tatctacatc ttctttgtca ggaatatctt ccgctgtggg acctaccagc
     1441 agatttccat ggacagtagg agttacaagg ataccttttc ccattttgga cgggagttgg
     1501 aaaacggtgg aattcaccag attaccgcaa tttttgtcca taagacggta ctcacctctt
     1561 ctggggacaa tacggatcct gtcttcacag atcatgttat ggatctcatc cgcatagaca
     1621 cctgccgcat tgataacgat gggagcaagg aacatctcac catccacctg cacctgatac
     1681 atccgtcctt ccttcgcagg tgggttctcg gtccataccg gtctctgttc cacgatagct
     1741 gtcaccttgt ggtcacgata gaactctacg ccgttttccg ccgcattttc cgccagcgca
     1801 atattcagtc cgaaggggca tacgatgccg ccggtgggtg catacaatgc tcccacgata
     1861 ttccgggaaa tcgccggctc catagcccat acttcctccg gagacagaag cttcaattcc
     1921 ttgaccccat tctctatgcc acgtctgtac agttcttcca gtccgggcat gccgtcttct
     1981 tcaaagcaca gtaccaggga accgttcctg cgaaacggaa agtccagctt cttcgacagt
     2041 tcctccatca tttcgttgcc ccggacattt aattttgcct taagggttcc aggcaccgca
     2101 tcataccccg catgcacaat gccgctgttt gccttggaag taccttcgca gacatcactt
     2161 ttcgcctcca gtacagcgat cctcagcttt cccttcgcca gttccctggc aatggcgcag
     2221 ccactgactc cggcgccgat aatgatcaca tcataatcct tcataacctc gccctgcctt
     2281 tcctatgttt acatttatat ttgaatgtcc acctttacct tctgaatatc cgttttctct
     2341 ttatgaatgt ccatttttta tttgtcgcaa atgtctcttt gctatttatt ttaccatatt
     2401 ctgcgggaaa acacgaccga aaaccagcaa cactctgtgt aaaatgtagt cgaaaaaacc
     2461 ggacagcaat cgctgtccgg tcagttttct cttataacaa tgtattcaga tccaccaggg
     2521 ttccgccctc gatctccacg cacttgtcgc tgtttaactg cagccatacg ctgcaggcat
     2581 tggggttata taccttttcg taatccgcag agaactgtaa acgtttcgct gcatccatat
     2641 aatcaatgat ctcgatgttg gtggcgtacc agatgtcgtc tctgccaccc atgtatttac
     2701 agaagttctc gatcacatcc cagttgtcat tattatcgaa ctcgtagctg tgcccccaga
     2761 catacatcag cttcaagtac tggctcttct tgaaatcagc gaaaaattcc gcgtactcca
     2821 tcagcttcgg atcattatga tggcaggtag catgccactc caacggatcc tttggcagtg
     2881 caaaatccgg aaccgtctct accactctgg cataagccac gcccaactga cgatacagct
     2941 gcttaatctc ctcgctgtaa gaaccgttgg gatacgcatg tcccctgacg atctgtcccg
     3001 tccatttttc caggcctttc cgatcctcca gcagttcctg tgccacttcc actaacggac
     3061 atctctcgat ggtggggtgg gtcatggtat gggttgcgat ctcatgtccc gcatacagcc
     3121 ctttcatttc ttcaaaagtc atgcgggggc gttctcccac cagcccataa ttcagattaa
     3181 acgttccctt gataccatat ttattaaaaa tgtcgatcag gcgtctgtcc tgtaccttac
     3241 cgtcatcata actcatggtc agtgctttgg cttttccgcc ggggaaacaa gtatatactt
     3301 tcatcttttc catccttttc tactgtaatt tcttttgtgg ttttaaaaat atgataattt
     3361 tcatcccaca tgttttcatc atattacgac tggaacaatt ttgcaactaa taagaatccg
     3421 gcattaaccc tctttatcgt gcagaacttt ccattgcctt cctttcattc atcctttaat
     3481 atagtaaaat atacaaccaa taaacgaatt acaatgagaa gggagcatat ttatgaaaaa
     3541 attcaactcc ttccgtaatc gccagaacgg ctttgaagat ttaggttccg gcaccgcgga
     3601 agcagcaaaa acgaaaaaaa gctggtggaa agtccccggc atcattcttg tgattctggt
     3661 aatcgctatt tttgctttta attctactta ccagatccgt gaacaggagc aggcggttct
     3721 gatcacctta ggccaggcca aagcggtaac ggatcccgga cttcatttca agataccgtt
     3781 catccagcag gttcgtaaag taaataccac cattcaggga ttttccctgg gatatgatgt
     3841 agattccaat tcttcggaaa ccgacgattc tctcatgatc acttccgact ataattttgt
     3901 caacgtcgat ttcttcgtag agtatcgttt cagtgacccg gtaaaagctg tctatgcatc
     3961 caaggatccc gtactgatcc tgcgtaacat cagccagagc tgtatccgta ccgtgatcgg
     4021 cagttatccg gtggacgatg tcctgactac cggaaaaaat gagattcagg ccgctattaa
     4081 agaaatgatt ctggagcgtc tgagcgaaca ggatatcggt atccaactgg taaatatcac
     4141 aatccaggat tccgagccgc ctacctccga agtcatggaa gctttcaagg ccgtggaaac
     4201 cgccaagcag ggaaaagaaa ctgccctgaa caatgccaat aagtaccgca atgaacagct
     4261 tcctctggcg caggcacagg ccgacggtat catcaaagac gctgaggcgc aaaaggccga
     4321 gcgcatcaat gaggctactg cacaggttgc gcgttttaac gctatgtacg aggaatatat
     4381 caagaacccg gccgtcacca aacagcgtat gttctacgag accatggaag aagtactgcc
     4441 tgaccttaag gttgtcatcg aaagccccaa cggcaataca cagaccttct atcctttgga
     4501 atccttcaca ggtacggcag ccggttcttc cacggaaaac agcacctctg aagctgcaca
     4561 ataaccattg tctacagaaa ggtagtttta atcctatgaa aaaattgaaa aatatatctc
     4621 tgattctatt ggtactggtg ctgctgatca taggatcctc cagcattgtc gttaccaacg
     4681 aaaacgaata cagtctgatc cgacagttcg gtaaagtaga ccatgtagtg agttctgcag
     4741 gtgttacctt taagattcct tttgtacaga gcgtcgataa gcttccgaag gagatcctgc
     4801 tctatgatct gtcttcctcc gatgtcatca ccagtgacaa gaagaccatg atctgtgaca
     4861 gttacatttt atggcagatc aatgatcctc tgaaatttgc ccagaccctg aactcctcca
     4921 tctccaacgc agagagccgt ctggacacca tcgtatataa ctctacgaaa aatatcataa
     4981 gcagtacgac ccagaacgac gtcatctccg gacgtgacgg agagctggct gcagccatca
     5041 tgaaaaacat tgggaacacc accgagcaat acggtattga attgctctcc ttcgaaacca
     5101 aacagctgga tctgcccagc gacaacaaag ccgctgtgta tgagcgcatg atctccgaac
     5161 gcgaaaacat ctccgcgacc tataccgcag agggaagctc cgaggcacag aagatccgca
     5221 acaccacgga taaggaggtc aacgtgctcc tgtcggaagc caataaaaac gcggaaattc
     5281 tcatcgcgga gggcgaagcg gaatacatga gaattctgtc agaagcttat tccgatgaat
     5341 ccaaacagga tttctattcc ttcgtcagaa gcctggatgc actgaaggct tccatgaccg
     5401 gcgataataa gacggtgatc ctgtctccgg attccccgat cgcacagatt ttctacggaa
     5461 gataacataa aggccggtgt ctgcaatatt cttgcaggca ccggcttttt tatgccagaa
     5521 tctcctcaat tctgcgtctc tcttcttcac tgaatgcggt tgcatgtaca ataccgatat
     5581 tatccaggat ctgtgagggc ttagatgctc cgatcagaac actggtaata tccccatccc
     5641 ttaagatcca cgaaagtgcc atctgtgcca aggtctgtcc tctctgggct gccagatcgt
     5701 tcagtgcacg taccttcttc aatgtctctt ccacgatcgc cgcttctttc aggaatctgc
     5761 cgtcggtacg gacacggctg tcctcaggaa taccgtggag ataacggtcg gtcagaagtc
     5821 cctgtgccag cgggctgaac gtaatgatgc cgatgccatg ggcagccgca taatccttca
     5881 atccgtcctt ctcaatatct cttacaaaca tggaatattt tctctggttg ataataaacg
     5941 gtgtccccag ctccttgaac agttctgctg ccgcaatggt ctgctcctta ttgtagttgg
     6001 aaattcccac atacaatgcc ttaccggatc tgacaatgcc atccagtgca cgaacggtct
     6061 cctccaacgg tgtatttgga tccggtctgt gatggtagaa gatatccaca taatccagtc
     6121 ccatgcgctt caggctctga tccaggctgg ctaccagata ctttttgctg ccccagttgc
     6181 cataagggcc gggccacata tcataaccgg ccttcgtgga aatgatcaat tcatcacgat
     6241 accttcccag tccttttgca aggatccgtc caaaattctc ctcagcgctg cccggtaccg
     6301 gtccataatt atttgccaga tcaaaatgtg taatgccatg gtcaaatgct gtgtggcaca
     6361 tgtcctgcat gttgtcaaaa ttcccattgg agccgaaatt gtgccataat cccagagaga
     6421 ctgccggaag cagtagtcca ctggcaccac agcgattgta tttcatttct tcatatcttt
     6481 tttcatctgc ctgatacatt taaatacctc ctttttccga aagaacatta tagacattat
     6541 tgtaactatt cagaccccat tcgcaaaacc gcctctgcga ggcttccttt ttgctcatga
     6601 ggttacttcg ccatataaaa ctgcttatct gcctgaatgc aagcattcgc agaacacagt
     6661 tttgcatggc tctgaatagt tacacattat tataaattta ttttcatccc gtttctatgc
     6721 aaattttata gaataaagtg caatatctta caaatcacct cttacgtccc acgatcttca
     6781 ggcggctgaa aaacaggtaa tacacaataa aagcgatact ggtcaccgtc caggtcagcg
     6841 gatagctgaa tacaatggtg agaatgtcat ttttcagagg tacggctgtc aggatccata
     6901 ccacgcgcag tgcgcaaatc cccaggcagc agatgatcat gggaagccag ctgtcgccga
     6961 taccgcgcag agtaccggac aaaatctcaa tggcaatata ggtaaaatat gtaggtacca
     7021 aaaaatgcag aatctgttct ccgatcacta ccacctgtgc atccgtggta aacagttgat
     7081 aacagatcga tgcataatta tagaggatca cggacaacag tacggttgcc ccggctgtaa
     7141 tccccataca ggtacggact cccttgtgta cacgatccat tttccccgca ccataattct
     7201 gccccacaaa ggtagtgatc gatatgccaa aggcattgac gatcatccaa aacaggctgt
     7261 caatcttgct gtaggctgtc catgccgcta ccgtatccgt tcccagttcg ttaacgctgg
     7321 actggatcag aatattggac gatgtataca tcaccgactg caatcccgcc ggaagtccga
     7381 tccggatgat cctgcggaac attcttccat caaaacggat ctttctcagt tccagacgat
     7441 gcatatcctt cgtccgcatc agcacccaca ataccagtac cgcactgagt gcctgcgaca
     7501 gaatggtcgc cagtgcggct ccagccacac ccatatgcag tccgatgaca aatgcaatat
     7561 ccagcacaat attcgtcagg cagctggcga tcaggaaata taacggtctc ttggaatccc
     7621 ccacagcccg caggatgccg gctcccatat tgtagatcag gtttccgatg actcccagga
     7681 aatagattcg gatataggtt gtcgccggtc ccaaaatatc ctcaggtgta gacatcgcac
     7741 gcagtgccca tggtgccgcc gtaataccca cgatcatcat gccgactccc gccaccagac
     7801 tgaacgcaat ggaggtatgt acggcatagc ctaccatctc ggcacgtttt gccccataat
     7861 actgggaaat aatgaccgtg gcaccgctgg acaatcctac gaaaaatccc accaacagct
     7921 ggatcagcgt accggtgccg ccacctaccg ccgacagagc ttcttttccg acaaaacgcc
     7981 ctacgatcac tgcatctgcc gcattatata attgctggaa aaaggttccg aacaaaatag
     8041 ggaaaaagaa caggagcagc tgctgccaga tgactcccgt tacgatcttg ttttctttcc
     8101 ctatagcatt ttctgtctgc ttttgtgttt tctttgtatt ttcttccata taaatcatgc
     8161 cctttgaaaa tctgtacttc tacgatgata gtatcatttc cacagaacct tttcaagagg
     8221 gcaatcttat ccacagctca gggcaggaaa atccccgggt ggtggaagca cccggggatc
     8281 ctgtctgcac ccggatgagt cgcagcatcc gggcacagcc ctgtatatac caaattgagg
     8341 gattagataa aagctaagtt aggggcaagc cactcccgtg caggcacgtc gtgtcttagg
     8401 gattataacc ctggcattat tactccacgt cttgcagagc tgcgaagtct tcttcgccgg
     8461 tacgaacctt tactactttg tcaacgttgt atacgaagat cttgccatcg ccgatatgtc
     8521 cggtgtacag agccttcttg gcagcatcga tgactgcgct tacgggaatc ttgctgacaa
     8581 taacttctac cttcacctta ggcagcaggg tcgcatccat ttcaacacca cgatacatct
     8641 cgccggcacc cttctgtacg ccacagccca tgacctgtgt catggtcata ccggttacac
     8701 ccagatcgtt cagagccttc ttcagtacct cgaaccgggc aagctttgca ataaccacta
     8761 ctttgtagat tccggttgca ggaattgctg ccggagctgt ctctaccttt actgccgctt
     8821 ccttcatggc tgcggaagct gtgtcataat cacttacgcc aaggctggta ttttcattta
     8881 cctccatggt catagtgttg gaaatatcca taatgctgaa gcctgcatat gcggaaggaa
     8941 gaccatgctc acaggagtcc agaccaacaa tctcttcttc ctcagataca cgtaagccta
     9001 cggtagcctt gatgatcagg aaagcaatgg taatcgttac tgcagtccat gccgccacac
     9061 ttacaaaacc taacagttgc aaaccaagct gcctgaagcc accgccatag aacagaccta
     9121 ccacactgtc attgccgggt gcggaagtgg ttgcaaataa accggttgcg atagtacccc
     9181 agataccatt catgcaatgt actgcaacag cacctacagg gtcgtcaata tgaagcttat
     9241 aatccagaag ccatacacca aatactacca gaagtcctgc tactgcaccg atcacgatag
     9301 caccgaagca atccgtcaca tcacaaggag cggtaatcgc taccagacct gccagagatg
     9361 cattcagaca catggataca tcaggtttgc catatttgat ccaggtaaat accatacata
     9421 ctacagttgc gattgcggga gctacggtag tggtaaggaa tacggagcct aactgctcta
     9481 cggaagtaca agcagcacca ttgaatccat accagccaag ccacaggata aatacaccaa
     9541 ggcatccaat cggaaggtta tgtccgggga atgcatttac ttttacaatc ttaccactct
     9601 tgtccttttc aaacttacca atacggggac ctaaaagctt tgctccgatc aaagcagaga
     9661 taccgcctac catgtgaatt gcgcaggaac ctgcgaaatc atggaagcca agctgtgaaa
     9721 gccagccgcc gccccagatc cagtgagctt caatgggata gatcaaagcg gagatcacac
     9781 cggaataaat acaataggat aagaacttgg ttctctctgc catagcgccg gatacgatgg
     9841 tagctgtggt agcacagaac accagattaa atacaaaatt tgaataatca aaattctcat
     9901 aggcagtaaa gatgtccagt cccggcttgc cgatcagacc tactacatct tcaccaagta
     9961 acaatccaaa gccaatcaaa ataaatacta cagtaccgat acagaaatcc atcagattct
    10021 tcatcagaat attaccggca tttttggctc tggtaaatcc ggtctccacc attgcgaatc
    10081 ctgcctgcat ccagaacacc agggccgcac cgatcagaaa ccagactgca aataattgac
    10141 catctactgc gcttaagatt tcttctgtca taacattttc ctcctcactc ttcgtgaaat
    10201 atcttccacc atacttcacc ggtgtaaaaa aatatgcgat actatggtct catccttcga
    10261 agccccattg catcgcatcc ttagggtata gcagttaaac tgttttccac atacccatac
    10321 gctatttttg tccggccacg cgcgttgtga tactgccgga caatacccgg gatatacatc
    10381 ccggtatgat tagcggtcgt caagtgacca ttcgtgccag cacgatgttc actttcctcg
    10441 tcgccataac aaaaagggcg ctacagtttt attctgtagc gcctttgcta tgttgagagt
    10501 atagcaccgt ggttttagag tgtcaacatg ttttttatat tttctttata tttttacagt
    10561 tccataagaa gtcgctcata gcaagttcct tcccaggtga cctgatccag ttgggaaaat
    10621 cccagtcttt ccgcaaggtg cagggatatt tcgttctctg gcatcactaa catctgcact
    10681 cgctcaaatc ccaattcttc tttcccgtat tttaataatg ccttgcatac ttccgtggca
    10741 tacccctgcc gctgccacgg gacaccgatg acgaagccca gctccggatc ctcgtaaccc
    10801 tcgcggttgg agaatcccgc tctgccgatc acctgtccgg tcatccggtc acagatcgtc
    10861 cagacgccgt aattataaaa ataatacata ttttccgtat aatccttcag ataggcctct
    10921 tctttcgccc gctccggata gagtccttct gtgtacttcg tcacagaagg atccgcataa
    10981 atctcataaa gggcatccag atcctccgca ccggtctccc ggatgagaca cctctccgtg
    11041 gtcagaatct cccagggaat gcccacgtat ctgcgataga ccttctccag ataatcaaaa
    11101 tccagttcac tgatattttc acaggcataa cggacaccgc taaaatcctg atccctgttg
    11161 tcctcatgga gccagacaag caccgggcag ccctgctcca agagttcctg tgcacgtata
    11221 ccgttatcta cgatccagag ttttccctgc ccgctttcat acccggacat catgctatcc
    11281 cggacgcttt tctcttcgaa gcactctttt cccgacccgt ttctttgttg ggaaaattct
    11341 ctttcctgtc tctgtctgcg aatcgcatcc agcagggcgt gcatctgcgc accatatttt
    11401 ttactgtagc tttttcgttc ctcgtccgat gcaccttccg gatatatttc tattgacacc
    11461 tgcttcaaaa aatccatctg cagcaattct cctctttacg ttttcccggg aatccattac
    11521 aataatattt atattttatg atgccagggt caaaaggtct aagcccacat gagcttgctc
    11581 ataggtgggt gaaagacctt aggacccgct ccgatatgag cataaaagtg ggatgataat
    11641 cccacgatat gcaaatattg ggtataattg tgagagtgcg tagcacgaag caattcgtag
    11701 gcatcaaagt cgggggcttt tgaccccgac atcattttat tctatttctg tcaagaaaga
    11761 aaggaaatta ttatggcaaa gaatcctatt gttactatta ccatgaagga tggcggtgtg
    11821 atcaaatgcg agctttatcc cgaagttgca cctgaatccg ttaacaattt tatcagcctg
    11881 atcagcaagc atttctatga tggtctgatc ttccaccgtg tcatccgcgg tttcatgatc
    11941 cagggcggat gtcccgatgg caccggtatg ggcggccccg gctacagcat caagggtgag
    12001 ttcgctgcca acggcgtaag caacgatctg aaacataccg aaggcgttct gtctatggca
    12061 agagctatgc atcccgattc cgcaggcagc cagttcttca tcatgcacaa aaccagtcct
    12121 cacctggatg gctcttatgc cgctttcggt aaggtcattg agggcatgga tgtggtaaat
    12181 aagattgcag atacccctac tgattacagt gatcgtcctc tggaggacca ggtaatggaa
    12241 agcgttaccg tagatacctt cggcgagacc tatcccgagc ccaacaagct ggacagataa
    12301 gatttcaaaa aaacaacctc cgtaggtcag atgttctctg acctacggag gtctttttat
    12361 gtctatggtt ttcatatctg tggatcaata ttccacgttt tcacaatttt caataaaaat
    12421 aggagcttcc acttcgccct tcatgtcctc agtaatctca taagagaaat tccggaaatg
    12481 gatattttcc gcattgcggg catacagcac tctgagagaa tcctccagga tcacattccc
    12541 ctcattggga cgcagatcat ggcaattctt cggccagtcc gtcttcttct ccagttggac
    12601 atgaataccg tcaaatgtga tatcccggat atttttcgaa tcgtcaccgt agatcaggat
    12661 gccattctct ccgacacagt tgatattctg gaaacggaca ttccggatat atcccacatt
    12721 cgtctgtgca tttctcttca ccgcagtgat ggtaatgggt tctgcctcgc cccaccaatg
    12781 taccttggag aacaggcggg tatcgatatt cagattggag aatgtgacat tttctataca
    12841 gcctttatca cgtaactgca gggaaatagc acggttggtg cgctggatac tacagttggt
    12901 cacggaaata ttccggaaag gtgcttcact ctccgtgccg aacttgatag cggcactggt
    12961 ggagatcatg gtacagttat ccactacaat attttcgcag ggaccatatt ccatggcagc
    13021 cgccgtattc ttaaatacaa tacagtcatc tgcgcattca atatgacagt tggagattct
    13081 cacattgctg cagtgatccg gatcaatgcc atcgcagtta gccagacgca gattattgat
    13141 aatacggatt ccctcaatca atacttcctt acatcccacc agatgagtgg tccagaatgc
    13201 actgccggtc agggttacct gctggatggt cagatgcctt acattttcca ggaacagcaa
    13261 cggtactctg ggataaaaat atccgtcaat atgccatttg gtcacttttc catagaatat
    13321 ctcctcgttg ccgtcaatct tgccaaatcc ggtgatagcc acattctcgg catccttgct
    13381 gtatacaaaa ttcagcgtcg gttttccatt gtaatcacag gcattgtagg tcggagtgtc
    13441 taccttgctg atctgtgggg ttcccacttt caacatatcg aaatcttcca gatggtcact
    13501 ggctttcagg acagccccca tctccagatg aaattccaca tttgatttca gaaaaatggt
    13561 tccgctccgg aagacatgtc ctccctgtag caggacttta ccgccgccag cctggctgca
    13621 ggcatcaatc gccttctgga tggctgcagc gtcattcgtc acgccatcac cttttgcacc
    13681 gtattccaat acatcgtata tcatctctct gctcctctct tagcccttca ggccggaagt
    13741 cgaaattcct tctaccagat atctctgcag gcagaagaag atcaatactg caggcaggat
    13801 agacaaggtt gccatagcga acatagcacc gtagtcggaa gaagaactgg ggtcacagaa
    13861 cattttcagt gccagactga caggatattt ctctgtcttg ttgacataca ataatgctcc
    13921 aaggaaatca tcccatctcc acataaagga gaagatacat ccggtgatca tggtaggaac
    13981 catcagcggg aagatgatct tggtgaaaat ggtataatag gagcagccgt caatctttgc
    14041 tgcctcatcc agctctctgg gaatgccttc aataaagttt gtcatcatat aaacgaagaa
    14101 accctgaata gcaaagcaat aaggcacgat cagcggcaga ttggttccaa cccagttcag
    14161 cttctgatac cagagatact gagggatcat cagtacctgt gccggcagca tcatagatgc
    14221 cagcattgct gcgaagagaa gctttctgcc ggggaatttg catctggcaa atccataggc
    14281 taccacagag gaagaaagta cggttccaat ggtggcggta atggagataa acagggagtt
    14341 cttaaagaag gtgccgaatc ctactttggc aaaacccttc cagccgttca catagttctg
    14401 taatgtgaac ttctccggga tcagactggt ggccgtagtg aagatggtgt tcgtatcctt
    14461 aaaggagctc attaccatcc ataccagagg gtaaatcatt accagtccga aagcacatac
    14521 taaaacatga tatataatat tactgattgt cttttttctt ttcataataa cacccactta
    14581 cgcattgcgg cgtacctttc cgattctata aaccgccatc gtatacccac agttttttgg
    14641 ttgcaaacag gatccatgta ataaatgcga tcaatgccaa catgatccat gccagtgctg
    14701 cgccgtagcc ggtacttccg aagaggaagg actgctggta catgtatacc gtgtaaaaca
    14761 gagtagagtt catgggttta ccctgtgtga tgatcaggca ctgtgtgaat gccaggaagc
    14821 cgttgatcat ctgcattacc aggttaaaat ggatcgtggg ggtcagaagc ttcagtgtga
    14881 tcttccagaa ctgctgccat ccgttggcac catccactct tgccgcttca taaaggctga
    14941 caggaatctg tttcaatgca gacaggaaga tcagcatgga agaaccgaac tgccatactg
    15001 ccagaatgat cagagtccag attgcggttc tggtatctcc caaccaggct accggctctt
    15061 tcattccgaa caccatcaaa agtttattta caagtccgtc taccgcaaac agtctcttcc
    15121 agaggattgc tactgctaca gatccgccga tgatggaagg cagataatac agtgcacggt
    15181 aaacgcctgt cattttcgta ctcttaaata aaagcagcgc aacgatcaat gcgaagatca
    15241 gtcgtaacgg aaccgaaatg atggcaaatt tgaaagttac caccaacgac tgataaaacg
    15301 ttgcatcgga aaacattctc ttaaagttcg cccagccggt aaatgtaggg ggctgtaaaa
    15361 tgttgtaatc gcagaaggaa tagtacagcg atatacacat gggaataatc ataaacgcca
    15421 gaaatccaac gataaatggc atagtacata cgatacctgc agccttttcc gtattcagct
    15481 tctgacggat agtcatatgc tttgttgtct tcataacgtt acctccgttg ctgcagcatt
    15541 catagaagca gccttttttc agaatgggta ctgtgggatc ttccacagta cccatttggt
    15601 ctttctaaaa atgttgtaat acttatactg tgctactgct tatgtaagcc ctggcagctt
    15661 atctggacat gatctggttg cccatcttga acagatcttc tgctgcctcc tgtgcggtca
    15721 tctgaccata gaggatctta tcaaccaact ggttgtagta atcatatact tcatttgctc
    15781 catcagggat cgcagggctg atagcggagc tgttgggagt tactacgtca tttacatact
    15841 tggtaaccac ctgtgcagtc tcatcctgca gaggagcgat ggcggatgct actacggaag
    15901 aagcaggcac acctctctca ccaagaagaa tcttgttagc atcttcagag ttcaacagat
    15961 aatttacaac cttaacagct tcagcctcat tctcgccggc atctctggat actgcaaaga
    16021 actggctggg ctttaagaag ttagacttct gagggttctc ggaaggccat gtggtgatac
    16081 cgatctccat gccttcggga gcggccgcta ccatagcgga cagctggttg gaccagaaga
    16141 atccgcacca ggactgggtt gcttcggaag agaagtatac cagaggactc tgctctacag
    16201 agttggaagt cagttcagcg tgaacatctg cgccgatgat ccatccgtcc ttgacaccat
    16261 cttcataaat ctggaagccc ggtaccagac ttgctgcatc tgttacattg agggaatcct
    16321 cattaaacag cttcaccaca tcgtgagaac gcattgcata gggcagatag ttgtcacctg
    16381 cactgtaagg aagatcagtc ttaacaccgg acttctcata aacctcacgt gccactgcct
    16441 cgaactcatc aattgtcatg ttatctttta cagtgattcc caggctatcc agcaaggtct
    16501 tgttgtagaa cagggaaggt gcattcattc ctgcacagat tgcatataac ttaccgtcaa
    16561 tggaacccgc atccagaata ctctgagata cattagatac atccagcgta ccatcttcta
    16621 cataaggtgt caggtctacc agcagatctg ctgctacata ctgtgccaga taggaatagt
    16681 ccatctgcag gcactcggga agggaattac ctgctgccag ggtggacagt ttggtccagt
    16741 aatcattcca ctctgcaggc tgtgtatcaa aggttacacc gggattctgc tcagaataca
    16801 gatccagggc tgcctgtgta cgttcatttc tggtctggtt gccccaccag gtgaatgtca
    16861 tggatacatt tccggctgct gcacttccgc tgttgtcagc agtcgtggtt gcggtattac
    16921 cgctgtcggt ggaagcggta gctgcgggtt cattggaaga accacatgca gtcagtccga
    16981 cgatcatggc ggatgccatt aacagggata aaactctctt tttcattaat ataaccctcc
    17041 ttttggtatc gaaaggtaaa tccgctatgg atttacttct ttttgatgtt ctaattataa
    17101 cagttcttat tttttccagg aatatacttt tccgtctatt tgcaccaaaa aaccgatatc
    17161 attgttgaaa acatgtgcat gttgtgtata taatcaataa agaactgatg gaggcttgta
    17221 tatcatgtca ttttttaata agaatatctc gagaaaaata gcaaatcaga agttagaaac
    17281 caagttactg ctctctacca tagggattga ccttctcttt ctgttctttt tcctggtagc
    17341 tgccttttcc attattacat cgcgttatca taggctgctg tatcagtcca tgcagtcttc
    17401 ctcgtcgtta gtctcctatg aatttactaa ccggctggag gatctggtga ccatgaccaa
    17461 tatcgtccgt tcagattcta cagtccagtc aacactggac gaaatctacc agccccagga
    17521 agactatgct gtacattatt actctgatat ttattctgct ttacagaaac actacctgga
    17581 ataccgccag ccctacctga aaatggcggc aatttcctgc cccagattca tcacttatac
    17641 caatgaaaat attgcctgtc ggccggatgc ggatctgact aaagaactga tagctctggc
    17701 agaggccggg gaaggctccc cggtatgggt aacttcccat gcggaagacc acggtatatt
    17761 tcttgtacgg gagatcaaaa agatcaagaa cttacggctg gataatctcg gaacattgat
    17821 catcaatgta aatctgggag atctggtaac ggaaatgtcc aagatcagca atgaatataa
    17881 agattcctac tggatcatct atgagaaaga ccagctgctg ttctcttctc cggaattgga
    17941 tacagaggaa gtacagaaaa tcaatgataa aatcaaaagt tatgctgttg tcaccctgaa
    18001 tggcagaaaa tattttgcca tccgcggtac catggacatc aacgaatgga attatctgca
    18061 tctgatctct tatgacgaag tggcaaaatc ccagcagatg accgcatttc tatatgtact
    18121 gatgctttcc tgcggtttcc tgtgttccat tctgatggtt cacgtaatcg tacgcaagat
    18181 caccaggcat tttgatctgc tgctctatcg catgcagcaa ttcggtgaag attccaccgt
    18241 actggcaccg gttccctacg attacaccga tcgagccgat gaaatcggta tcctacaccg
    18301 gcaatttacc catatggcgg aacggatcca gacattggtg gtcgagaatt atcgtcaaca
    18361 gcttctgacc aaagatgcac agttaaaaag cctggaatcc cagatgaatc cacatttcct
    18421 gtataacacg ctggatgcca tcaactggag ggccaaggct atcggagagc cggaaatctc
    18481 gcagattgct gactctctgg gacatttcct gcggatgacg ctgaacaaaa aagaacgctt
    18541 tacgttgaag caggaacttg atattataca ttattatatg actatacagc agcttcgttt
    18601 tgacaaccgt ctgaatttta cggaaagcat tccggatgct tgtctggatg cacaggttcc
    18661 caaactttcc attcagccgt tgttggaaaa tgccgtacac tatgcactgg agcagatcac
    18721 ggatgaatgt atcatcaatc tcacctgtgt gtattcccag gggctgatac agatttatgt
    18781 gaaaaacagc ggatctgaat tccccgatga tttattgcag aatctccgga cacataagat
    18841 tcaggaacag ggtctgggca ttgcgttgtt aaatattgaa gaacgcatcc agctgatgtt
    18901 cggccagcaa tacgggctgc acttctataa cgaaaatgat tttgctgtag taaaaatgga
    18961 gataccttat gttaaagatg attatcgtgg atgatgagaa gatcatacgt gaaacgatcc
    19021 attccctgat cgactggaat tctctgggaa ttgatgtggc tgctgtctgc aaagacggta
    19081 ttgaagcctt tgactgtatt ctggatgaat acccggatat tgtcatgaca gatatcaaga
    19141 tgcccggttt atccgggctg gatctgattg aaaaggtccg tgctgcccag ttgaacacag
    19201 agtttatcat cctctcagga tatggtgaat ttgaatttgc acggaccgcc atgcgctatg
    19261 gtgtgaaaca ctatctgtta aagccttcca atgaaacaga gatcacgcag gtcatcgtat
    19321 cctgtgtgga aacctgtaaa agcaagactg cttcccgggt agattccttg ctgacacagc
    19381 tgttcctcac ggaaatgcca ccggaaaaac ttttgcagag aagatttttc acggaattat
    19441 ccggggaaca ggatgtggat ctggccaaga cccagatgat ccgtctggta atggaggcct
    19501 ccaagaagga atatttcccg ctcagcaaac tgcagaccac agacagtctc atggcagtca
    19561 acgtctgtgt caccatgaat gacctgattc catctgcgga gcagatcatg acctcgatct
    19621 tctctgtgga accgcagcac aaatacacag actgtgtgga aaaggtcata aagtacatcg
    19681 gagagcatct gagtgacagc aacctttcac tgaaatggat ctcggaaaat tatcttttta
    19741 tgaatccgga ttatgtcagc aaaatgttcg tcaaacagac cggcagcaaa ttttccgctt
    19801 atgtaacaga gctccgcatt caggaggcga aaaaactttt actggaacac tcagaagaat
    19861 ctccctatgc ggtagcggaa atggtcggtt tcggcaataa tccccagtat ttcagccaga
    19921 tcttcaagaa atatacaaag ctgtcaccga aggattatgt gaagtcgatg ctggaaccgt
    19981 aagaccgccg ctattgcagc tattcagaac cccattcgca aaataggccg ttgttcatat
    20041 tttgtttaca aatcattcaa ggaattttca attccacaac acgttttgga catttctgtt
    20101 tcatatacta taaccataga aacaaacaaa acaaaaactt aagcacacaa cagtttcttg
    20161 tttaaaaaac tttttcataa taaatgccaa ggatcctgat ccttggcatc ctccctttct
    20221 aggggttttt tcaactatcg cagagcaatg cggatagtta ttttttatct tgttctatat
    20281 gtaagtaact ttatccctgt aaatcattcc tcatataaaa aatcaaatta ttaacatgat
    20341 gtactatgtc ttcttttaca acgcctaaat tgcaataata ctcattatgt aaggcaactg
    20401 aaagctctac ataactattc cacactctcg cagatgcaaa caatctcaac aagtttttct
    20461 tcccactttt atcaaattct tctagttttt tatgtagctc tcccatttct tgcaagtact
    20521 ctttgtgtcg ctttattcta tactcccaat tactaatctc tatttctact tcatgcattt
    20581 cgttgtatgt atgctggctc tcttgcatta tttttagtcg gtcctctgca tcatgcaatt
    20641 tcatttccat aattacaatt ttgttttccg cagttccacc caattgacaa tcctgtaaat
    20701 aatcagactg cgttatacat gtatctataa taggtaatat gctcgccaat tcataatata
    20761 tctgttttct ttcttcaaaa tatttttctt ttttaacatt tttcaatgcc caaagggacc
    20821 ctaaataggc tgctataaat gttccagcca ccgatattag ctctatgata taattctcca
    20881 tctcttatcc tttctggaaa ttttttatcg tcgcatcaat atatgttgta ccctctactt
    20941 tttcaaggca caagtctcat ttctataaca tctataacat aataactcta tttttcttag
    21001 ttgctataat cttttcctgc ttatctatgt aaatgtctac tccatcttct ccaattttct
    21061 caacaaaaaa agttttggga atgtaatatc ccaatgtctc atctttcttt attccaagat
    21121 gtataattgc attttcatcc tttgtataaa acaaaatatc gcagtcaatt tttgttggag
    21181 gacttacatt ttcattttta aaattaactg caaccggatt ttctaaaata tccggcatga
    21241 gatagaaata aacaaatttt gcctttatgc ttttaaattt gctcttattc atccttttca
    21301 gatcattaaa acaaagacct gtattctgta tataatccca gccgccttgt cccttgtaat
    21361 ggtgtatttc tttcttcgaa actgaatatt ttacaatcga ttcaattcct aacaaatgac
    21421 agaagttttc ttcatcaaac cttaattgaa tagaactctg cttctcattt tctgttatat
    21481 catattgata aatgaacgga tttaaaaatt ttctatagta gtcttgcaaa acatccaggt
    21541 caatatcgtt aattttgggt tttattaaaa gaccacacaa ttcttcggct ttgtacaaat
    21601 taatctcctt ttacaaaaaa gactctgaat ctcttcagag tctttttaag caactttaat
    21661 ttaattctgc tcatatttaa actctccgca gcggagaaat caggagttgc atcccaatta
    21721 tataaatagc atatgctact tatttcaaaa atatttctta tataagtata gtataccctc
    21781 aaacttacta agtcaagctt tttatctcag aattattcag ccttgccaac agatccgaac
    21841 agttccatct tctccttaac agtagccttg atagcttctg
//
LOCUS       DAWDHD010000028        21328 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_178_length_21328_cov_10.974287, whole genome shotgun sequence.
ACCESSION   DAWDHD010000028 DAWDHD010000000
VERSION     DAWDHD010000028.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 21328)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 21328)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..21328
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_178_length_21328_cov_10.974287"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         4955 a         5609 c         4034 g         6730 t
ORIGIN      
        1 atccggatcc ggaattccag cagtatccag gtatccttcc agagtatctg ccgtgatccc
       61 tgcaggatcc acttctccac tctgtacttt tgcggacagt tttttcactg cacggacaat
      121 ctcatccctg cctccgtaat taatggcaat ctggaaatgc aggccatcgt tgttctttgt
      181 ggcctcttcc agttccgcga tccgcgtccg gatatcctca tccagtctgg tcttgtcacc
      241 caggactctg acacacatac ggttcttctc cgctgtcttc agacaggtct tcatataatt
      301 gcggagcagc ttcatcagcg cgtccacttc atcctgcggg cggttccagt tctctgtaga
      361 aaaagcatat actgtcagat actggattcc catacggtac gcctcttcac agatcacttc
      421 caccgtcttg gctccctgca tatgcccgta attgcggggc attccttttg ctttggccca
      481 acggccgttg ccgtccagaa taattgctac atgattcgga attttcagat tctcttccat
      541 agttttaaac cttcttaaac cgtcatgatt tccttggact tgctgtcgat cagagcatca
      601 atatccttta catacttgtc ggtaagcttc tgcagatcat cttccagacc cttgatctca
      661 tcttcggata cttctgtctt tgccagcttc ttgaaggcat cgttgccgtc tcttctgata
      721 ttacggatag ctactttgcc ttcctcaccc ttcttcttta cttcttttac cagatctttt
      781 ctgcgttcct cggtaagctc cgggaatacc aggcggatca cactgccgtc gttggaaggg
      841 ttgataccga tatcggaagt catgattgct ttctcgatct ctttgatcag agacttctcc
      901 cagggagcga tctggatgat acgtgcctcg ggaacggtta cattacccac ctgctgaatg
      961 ggggtaggag ttccatagta gtcaactctc agtttatcta atacatgagg gttcgcacgt
     1021 cccgcacgga tagcagcaaa atctgcctcc agaaactcat aagccttctg catcttgtca
     1081 ctatatacct gtactcttgc gtccattgtt ttcctctctt ctgcgctctg cgcctttcca
     1141 tttttatttt ctgaaattat acggttacct tggttccgtt gaacttgccc ttcaaggtat
     1201 tcacgatgct gttctcttca ttcaggccga atacccacat gggcatcttg ttgtctctgg
     1261 ccagaatgga agcggtcata tccactacct gaagattctt ggcaattact tcatcaatag
     1321 atacttcatc atatttcttc gctgtaggat ctttggcggg atctgcattg tagacaccgt
     1381 cgattgcctt agccagcaga atgacatctg cctctacctc aatggcacga agaacaacgc
     1441 cggtatcggt ggagaaataa gggtgtcccg taccacctgc gaagaacact accatacctt
     1501 tggcaaaata tttatttgct ctgtccttgg aaaacagctt ggtaaaggat ccgcactcaa
     1561 aaggtgtcag gatattggtc atcatgccta cggaacggaa aatctcggag acataaatac
     1621 agttcatgat cgtagccagc attccgatct ggtctgcctt ggtacggtca atgttctcac
     1681 tggaacgtcc tctccagaaa ttgccaccac cgataacgat acctacctga ataccgtcat
     1741 ctaccagttc ctttacctgt aatgctacct ttctgacggt ctcctcgtca aaaccggtct
     1801 tcttctctcc tgccagtgct tctccactaa gcttcagtaa tattctcttc atggtcatat
     1861 gctccttatt ttactttctt tttctcgggc ttgtaatcat cgaagaagga agtcgggctg
     1921 acatccgcat agcactgaga gcacatacct tcgattacgg caccgttgtt gatctgtacg
     1981 gacttggact tgatgttgcc cattacgatg gcggaagaat ccagcactac aggtccctgt
     2041 acatcaatat cgccctttac cgcgcctgca atagctgcgc tgacggcagt gatgttgccg
     2101 atgactacag aacttgcacc gatttttaca gaaccttcgc tgacgatctc accgttgatc
     2161 ttggctccct ctgcaaagat ctcggcagcc ttggaatttc cattgatata tccggtaata
     2221 ttcagtttgc ccagaatatc aatatttcca ttgatcgtac ctaccagatc catagatcct
     2281 tcagaactta catctccggt gataaccata cctgcggtga taactgctgt ttcatctgat
     2341 gcgatttttg cgtccattct cttctctgct cctttgctat tctctgtatt ctcctgcttc
     2401 agaacactgt ccacagtcat atcgttctga agtatactgt ccagatccag ttccggtacg
     2461 gcctctccta tgggagcttc ctctatccgg gattcttctg ccgttgtttc ctcttccggc
     2521 tgttcttctt tttcctgggg aatttcaata gaatccaggt tcttcaacat ttcttccaat
     2581 gctgcattgt catttggtgt ctcttccgcc tgcggctcca tcggagtctc ttccgttgtc
     2641 tcctgcgtat tttccaccgg ctgctccttc gtctcttcca cggctgcctg atccagctct
     2701 tcctccggca tcagttcatt gaccgcctgg gacagatctt ccttcaagtc tgagaaaaat
     2761 cccatgatta tgtacctcca tttgtttctt gttatttatc atcggttttc gcgttctgcg
     2821 aacgatcaaa accgcaacag gcattctctt cttacaggta ttcctactct atatgatgaa
     2881 tcggcctggt attttccgta atcggcagga tcactgtatc cggccgggcc agaatattct
     2941 ctatgatatc cggcaaagct tccactgtag tcggcttctc cgcggaatca tgcaacagta
     3001 tgatagacgt gtctctggta tcgatatcct tggtacagtt ttccagtaat gtatccgtgg
     3061 ataacttcat gcttccgccg tctccggagg acacattcca gtcgtagtat tccactccct
     3121 gactgtccag ataatcgata aaatcatgca tatcgatctc gctgatcgta ttggagcttc
     3181 cacccgggaa ccggtaacag acactctcca ctccggtggc ctgatagatg gagtccctga
     3241 tccgggcaaa atcctgtgta aaactatcca ttgattcgta caattcctta tatttatgac
     3301 tgtatgaatg cattcccagc gtatgtcctt cgtcaacgat cctttgcagc gcctcttctg
     3361 cattggttcc ttcttttccc accacaaaaa atgtggcttt gacattgtag ctgtctaaaa
     3421 tatccaggat gtcattcgta taaatactgg ggccatcgtc gaacgtcaga tacaccttgt
     3481 gaatttcttc ctgcccggag ctttcctccg ccaccggctc tgaagcttct gcaccggaac
     3541 tgtcctcctg tatctgaggt gtctgtaccg gtgtactctt ctgctcctgc aactgctgca
     3601 gcagcgtctg taactcctga ttccggatat ccagttcatc cagtctctgc tccagtgtct
     3661 tcaaagttcc gtcaatcgta tgcagcctgc agatcagata cacacagcac aggagcggaa
     3721 tcacgattcc caccgccacg atacctatga tcatcttctt caaccgctgc actctttttc
     3781 gccgggcttc cggcaatgtc tgttgcagtt cttttgccat gaatctgtta acgtctctct
     3841 tcctatttcg aaaatctcag cctccatcag acaagacttc tgtaacagta tagcatattc
     3901 cacttttttg ggaaagagat ttatgtaaaa tgttattttg taacgcagga agccgcagga
     3961 gatattgcta tctctgcggc ttcctgcgtt ataatttcat tcccctgttt gtattccccg
     4021 tatgcctggg ttccctaccc aatttcctgc attcttcgtt ccggataatg tcccataatg
     4081 aaattatgga aaaagagttc cggaagatgg tgactccgaa agcgatacca gatatcctcc
     4141 ggtacattat caaactgcca gatctgtccg ttgtttgcaa attcgatttc caacatttgc
     4201 tgctgggcat cgtacccggc agacttgatc atgggatttg cccacattat tcgcttcatc
     4261 ttttgttctc tctttctcat cctgcttgtt gtcacccctg acataaatgt ccattcgctg
     4321 taaggcgttc agctgtatct ttttcctgaa agatttcccc ttatactttc acgtgaagat
     4381 ttttgtccca cccactgtga caattgcagt atattttatt tttcccttcg ttttccctta
     4441 tgcaggtttt agcatacttt ctgaatacat tgttgtatcc ccacccccag tggttcctgc
     4501 tgcaagtaat atgtactttg tatttctgca gttcatacat tattaagttt tttcgtaagt
     4561 gtagcaaggg tttattgtgt gttatttgtt acctggtagt tctaattgtt atagtacgaa
     4621 tttgaaggca ccggtgtcgt tttctacttc ttcataaacg tcgtagttgg cgtaatggac
     4681 acatgtgaca cggtaggttt ttccattgac ggcatcggga taaatcagac caccaccaaa
     4741 cacttcttca tcatgatttt ctgcaccatc agaagtcatt acagttttcc atccatacca
     4801 cattttttgc tgtactttaa tatcctttac accaataact gatgaaactt ctgctgccga
     4861 agtagtaaag gtcatttcaa gccccttagt tgtaaatgcc aaaattattt cacaatcaaa
     4921 cagagttgtt aacggttgta ctccaaatga gttatagcct acttctttat tggctattgt
     4981 tacatcaaca tttgccattt cactttgtat attttcttca gcttgcactt ttagtcctga
     5041 cataggcgta gcaaaaatca ttaccattaa taatgctatt gctgtaacct tccatttttt
     5101 cattttccct cttctcatta gtcagctcct ttcttttata tacaatggtc atgctacact
     5161 ctggagatat tgatgttgtt ttccttaaat atctctctat atacttcatg acatataatt
     5221 caattttctt acatttttta taatatattt tttcaacgta ttttttcaaa ttttccaagc
     5281 agtaccttcc ctcctttctt tgtctatttg tacatgtaaa aagaagctta gaaattcttc
     5341 caagcttctt ttcacttact tattatggtg gttcaaaact acattcttat actctactac
     5401 tatatcttgt aatacacttt tatcatttct tcccataacg atatattttt ctttaccata
     5461 ataaaaatac atcaaatatg tattttcccc cgatatctct tctttagaaa aaacgtcaaa
     5521 aaatattccc tcatcttcaa actctgtttc aaaaacatat cctggatatg tttctctaac
     5581 tctcaaaatt tcctgtggat atatttttat ttgaaatctt atattgtttt catcattttt
     5641 cataagtact gataccgtat catcactttg atccttatgt attttggcat agtcaaatga
     5701 ataatccgaa attgttttaa tttcaagtag ttccctcacg tactcactgt attcgctagg
     5761 tacttcttct atactatagc attttcccac ttcaaaattc acaaaatcct ccgcgccttc
     5821 cggtgccgta atcatagtcg tcccttcctc gttcttatcc atccaccaga agaaacctcc
     5881 atgcttctcc gcattggcaa ctacctgcca ggaaccgcca agtatcatta caacaagcag
     5941 cgctactgtc accactacaa atctgcgtct gtgaaagtac cagagcactt tatgaagctt
     6001 accctccctg ggcttgtcct cctctttctc tgcaacaggt tcacttttgt ccttctcttc
     6061 ttcagtgcca agcaacaggc gctcctcttc ttccctttcc tcacaatgtt tccagaaatc
     6121 atccaatgcc tcttccacag gcaattcatc ttctacattg tcttcctctg ttttcaggtt
     6181 gtccagcagt ttcattaatg tctgtacttc atccgcatca aactcttcat ctgatgcttc
     6241 cataatgtac cagtccaacc gcttggtaag ctcctgaatc aggctttcgt tatcctgctt
     6301 cttcatttta attccaattc ctttcccttc tgcatatcct tatctggtgt aatattgatc
     6361 cctcgttaag tacatgattc ttttttgtca attcttacag attttttgta ttttttgtcg
     6421 ataccggact cgtcgggtat gttttgggct atcttgcttc tcaatctgct gatcctgaca
     6481 ctcatattgt tctcggatat tttctcaagt tgtgccagct cagaaattga atacccgtga
     6541 agaaaatatt tgtaaaatct gttcttctcc tcttccgtca ggcctttcgt tattatgaga
     6601 tcaagttctg attttccgta accgtcttcc gtgaatgtat attcgtcggc atccaggtcg
     6661 acttcacatt ctatcttctt caatgactta ttcagttcac gtattttatt cttggcagta
     6721 ttgatcagcc atcccattgg attggaatgt tcacagagaa tatcccactt tttatacgca
     6781 acataatacg tttcttgcag gacatccttt gccagttcct tgtctgccac cctgtagcgg
     6841 ataaacttgt atatttccgc ctgtgtccta cggtacatct catcaaattc ttgatggtct
     6901 cctgttgtct cttctttctg catgtgcacc taccatcgcc tcatatagag gttttttctt
     6961 tctaaaatat ttactctata atttaacatt tatccccgta ctaaatatag atgattttac
     7021 tataaagtaa aaatttgttt tacatctgct tatattttac ttttcgtaga ttttgtgtca
     7081 attttttctc atcgacaaat actgttaaaa tttgcaattt ttgttgttga caaacggtat
     7141 ctcttttgtt agaatgaatc acggtgattg cgttagccgt cacgtgatgc tgctgtggct
     7201 cagtcggtag agcgtcgcat tggtagtgcg gaggtcacgg gtccgattcc cgtcagcagc
     7261 tctcatataa gaagcggaaa cattgatttt acagtgtttc cgctttttgt ttttcacaaa
     7321 cgctgtagat tttcttactg caaatctcat ctctgtcaca aaattataaa tcctccaata
     7381 atacatgtgc cggtatctcc agcacttcag caatatcaaa tattacctcc aaagaaaacg
     7441 gctggtcaca attcggtgcc tcgatcttgg tgagatagct gatactaata cttactctgt
     7501 ccgccaactg ctcctgtgta agtcctcgtt gttttcgata ttttttaatc gcctgtccta
     7561 taattttata ttgttccgaa tgtttctgac tcattccctc atctcctgtt tcctacctct
     7621 acaattctaa aggataatga aagaatgaat gtttcagtca taatgaaagt tatgggtaaa
     7681 aaaataccga tttattttgc atccgtttcc aatgcctgat aaatcggtat ttcataaaca
     7741 attattttct tcctaatagt ttaatctctg cgtaatgtaa atttgcctac cacttcattt
     7801 aaggaatatg cctgtgccga caattcctcg ctggttgcag aagattcctc cgcagatgcg
     7861 gaattggatt gtacgacctc cgaaatctga gtcactccca cttctgcctg ttccattgcc
     7921 tgtgcctgcg ctgttgattc ttcactaagt ctcctggaat ctgctgcgat ctccttaact
     7981 ccggtcacaa tctgctccag tgattccgct gccgcttcag cggcctgatt tcccctctca
     8041 atttcctgca gagagccttc gatcagactt ctggtatcca ctgcggactg tgcactctgt
     8101 tctgccagtt tgcgaatctg ttctgccaca acagcaaagc ctcttcctgc ttccccagct
     8161 ctggcagcct cgattgcggc attcaatgac agcaggttgg tctgactggc aatgtcttcg
     8221 atttctgcaa taatattctg aattttttgg gacgtatcgt tgattcgatc catctcttcc
     8281 ataagagatt tcatctgtgc atgtccggca tccgcctgct gtgcgtaatt ttccgccttc
     8341 cgggtggtct tctgcaattc acccgccgta cggttcactg catccgcgat atttgtaatc
     8401 gttgctgtca gttcctccac cgaacctgcc tgatcggtag ctccctccgc aagggactgg
     8461 gcagattctg ccagattctc agcgccggct gatacctgtt ttgcagcttc ttctacctga
     8521 tgcagcgtac cgttcatctg acggttcatt ttacgcatgg catccttcaa tgccacaaac
     8581 tgtcctacat atttatcctc aatcttagtg ccgatagcat aatttccctc tgccatctgt
     8641 cccagaagtt ctccggcatc cgtaatgatc atatccagat ccgatgccat cccctttgcc
     8701 acctgtatca tataagaaat ctcatcttcc gttgcacact ccggaaatgc gctgtttaaa
     8761 tctccctgtg cgaaggtctg cagacgatct gccaatgcta tcatgggctt ttctatgtct
     8821 tgcgccattt ttcgtcccag gatcaccgaa ctccagaaag aaaccacaac gattgccagg
     8881 atcaccaaca gcatgatccg ttccattgcc cgcagatccg cttccgcacg gtcacctttc
     8941 tgcacgcaca ggttcatcag ttcaacgaat tctgcataaa tctgttcata ttgagccgtc
     9001 aaatctgtcg tatccagttc ctgcgcttcc agataaccat ccgcatcatc cgaagttgcc
     9061 aattccaaga ttcgtgcctc cagctcccac tagccgtcta gttccgtcaa gacggcattg
     9121 taagcttctc ttccctcgga gaatttgagt gttctgctca attcatccat ataagtttcg
     9181 aatgcctctt tcttctggtc atgcagagct gtctgctttt cgatcacagc ttcatcatca
     9241 tacccgacca ctgcgcgcaa tgcacttcta ctctccgaaa aagcggtcat cgccttaccg
     9301 atatccccct gtgtaaaacc ataccgattc agtgcttttt catactgcgc cgcagcaatc
     9361 agaagcgctc cgattccaat gattgcagct accgctccaa ttaccgccac ttttgtgaat
     9421 ccctttgtca atcgcttctt aataccctgt tgcttttgtg ttgccatata tcggtccccc
     9481 ttgttattta taaaaaatgc cataatttgc attttcatgt aaattgtagc actttctgtt
     9541 ttcctcttca actgtacgaa ggaatcactt ttctcatgtt ttctggtctt tcattatctc
     9601 ttccgacaaa acctctatcc tatgtcttga catcttatca caatcttttt ttatgtcagt
     9661 acaatagttc aaaaatttct ccttatctgc atggtacagt aaatacatga tattataaca
     9721 ggtatgatat ctattctgat ctacgtcccc cacaggaaaa gacgtcttat tatacagctg
     9781 atccactttt gtcattatga tctcatggta ataactgttg ctaaaataat ttctgagttt
     9841 atatgaaaat tggtacgctt tttccccgtg ggttgtgatc ctcaaatgtt ctacatatgt
     9901 ctcaatcaaa aattctttca tgttttcatt ttcttccatg tatattattt tcaccttgtc
     9961 tgaagacata tggaataaat cataagagtg cgttaaaatt tcttcttttc catctctgac
    10021 tgcctgtttt agcattttgt ttaacccgtt tttatatatg gttaaaacat cctcattatc
    10081 ttcctccaga atatcactcc ataccatata ggaatctgca aattcgttta attccacaat
    10141 gttttttgcc tgaagcaatc ccttttttaa atccggcagc attactttta tctctgcatc
    10201 tgtcttataa taattgggtt tatctcccga tttaagaaac agtcgatact cggcttctaa
    10261 ttgttcttta tcaagcgtcc cctcctgata gtatctcaac agcattccgg tcaaattatt
    10321 actgctcttt gtaccataaa gatatttccc tattctatgc tccaacgtat tttcaatact
    10381 gctcaccatc tttttcaccg aatccgtatt gttgggatca tcctccttat aatacagatt
    10441 gtgtgtactt tcaacgacga tggcataaca tatcagtctc agttcctcca aaaattcatc
    10501 tttattgcag tcttcgcaaa ataatattac atcatcataa aatcgctgcg ccttttcaag
    10561 tgtccgcaag ttttccactt tatgaaggtt tagaaatttt tcaataaatt gagcatggat
    10621 atgcaattta ctccactcga cactctctgc ccgttccgta atagcatatg ttcgttcaat
    10681 taccttttca ctgtatttgt caaatatctc ttttttatct tttgacattt ctgcggtatt
    10741 cgcaacgagt ataactttca cataattaca ttgtttcagt tcctctatga ttccaaatat
    10801 ctcttccaaa ttcatattac tgttcattct ttccagatcg tctatgacca caatgtgaag
    10861 gaatgtaaat tctttggaca ataataaaaa tagttctctt tcgtttgcta tattttgtac
    10921 cacatcccgg gcctgtccga ctttatccca gacctttgct gcgccttcta tgatattatt
    10981 agctatttca ccaatcttcc cgccgacatt attcttcaat gccagttgaa acaatacttc
    11041 atgatatatt tgtctcgcat ctgtcattcc gaacattgat attttacaaa cgtccggatt
    11101 tcctgccaac gcctcattta ccgcataaga cttcccgaca ccccaggttc catcaaacaa
    11161 gatggtctta taaggtaagt cattgatatt gccaattata gtttttattt tatctgctct
    11221 cattttttcc tctctgtaca caaattcata ataaccggac aatcttttta cataagtttc
    11281 tccggttatc tgtcgttctg cgttgtgtaa ttgttggtga gttccggttt gcaaacggat
    11341 tgtcttataa atgtcacaga ttaatcattc catattttct ctgcattgcc agtagcactt
    11401 ccggatcatc cagctgcgcc accttatctg tatctcctcc ggcaattccc tgtaggattt
    11461 ccgatccctt gcatacatat ccagccgcac cggcttgcca tccacggtat actgaaattc
    11521 cctctgtggc tggatatctt ccagtgttcc cacccgtttt tccagcatcc gctccaatac
    11581 atcatggcag agcaccttgt cacccattgt cttgccgaag ataaaatcat ccgatatcgt
    11641 cagttcctca tagcgttttg ccattcacat tctcctttgc ttttaccgca ggaaaatatg
    11701 ctgtcgcaga aacgaatttc aatccgttct atgtcttctc ctgctctatt gttatcattg
    11761 ggtaattaag cagggattcc tctgaagtca tagacaccgg cttacaccga tatagaaatt
    11821 cgaaatagaa aatctgctta cgaatagagg atagcaaatg agcactcgat tgtcaacgtg
    11881 gcaaaatagc aaaatctgtc cctggcgaat ttgggcatta tgctttaaaa tcaattttaa
    11941 ctccatctat atatttattt ttgtcttata gcctgtcctt cagatttttc tgaacatctt
    12001 ttcataatca ttctccataa tgtaaatcct attattgctc cagggacatt atgaaacgga
    12061 tcatcaaaaa gttcaaagag ttcacgtttt gttaataact ggctgaattc tatactgtaa
    12121 cttataatta ctgcaatcaa tatcacccat tttaactttc gaacacctaa tccctgtaat
    12181 cctattccta ccttattgct ttaatataac tccaaaatga cataagttct gccctcggat
    12241 ttcttattat ttaacgggca tggaatcgtt cttcatgttg tgtacataga tcaccgtatt
    12301 gttgtctgta aagtccttac tgttcagatt ctccgtatat atgcacccgg aatatccata
    12361 cgaaccatca atattataca taaagtaaca ggaattatcg gaaggatgct gtaaaatcgg
    12421 atattcaatt tctgtccccg gtacagtgat ccaggcatac acatccggct tcaccgactg
    12481 ccgctcctgc cacaatggtc attatctatc gtttcatgtg agctccttct tcccaaataa
    12541 tggaagattt tactactcct aaattttatt ctgcgttttt aacgtaattt taattccgtt
    12601 ttaaatgtaa ccgttatata cgttcaaaac aggtattgtc aatagtttgg gggcttcatg
    12661 caggacgaga tggctggaaa aacagaagaa aacggatact tcgggtaaac ctccgaagta
    12721 tccgctattt tgactatgtt aaacaattcc gttctaatta aatccgaaca atttctggca
    12781 gatcttatat ccctctaccg aaatcttccg tccgcctctt ccgggaaggt tcatggtacc
    12841 gcccataatg ccgttacgca tccacagata caggcgtttg tcttttcctt tcaggtactc
    12901 ccacagttct ttctttttct ccagatgttc tgtggtaccg gagcggatca acagtacgga
    12961 agatactgcc atgatgatct ccagatagtt gcgcatgtac tggtacagac gtcggttttt
    13021 caccagttcc gactttctgc ccaccagata atcaatcatc agcttcgtca ctcggatctg
    13081 ctggtcgatg cgggagatca tcacggtctc attcaccgac tgatcctgtc ttcctatata
    13141 atatctgtag aaattcacat ccagataata catattcttc acataaggaa gcggttcaaa
    13201 tacatacagg ttgtccacat agaaggtgtg ctccggaagc ttcagtccac attcctgtaa
    13261 cagtctggta cggaagatca cggagtgcat cagtatgtaa tggcccttca gaaaatgatg
    13321 gcagtccgtc caggtgaaca tcttctccac cggcaggata tgacggtact ggatcacctt
    13381 tttgcggcgc tcgccttctt tttcatagac atagttgctg atgagcatat ccaacgcctg
    13441 accgccgccg gccagttctc tgagagtatc caggatctta aaatatgcat cctgcttcac
    13501 ccagtcatca ctatccacca ctttaaaata tagtcccgtg gcattggcga ttccggtgtt
    13561 cacagcagaa ccatgtccgc cgttctcctt atgaatagca cgcacaatcg taggaaactg
    13621 tgcttcatat tcgtctgcaa tctctgcggt acggtctttg gtagaaccgt catcgatgat
    13681 caggatctcc acatcctctc cgccgatcag cagggaatca atacattttc tcatatactt
    13741 ttcggaattg taacagggaa ttgctatgga aagtaatttc atatcgctgt cctttcttca
    13801 ctatcttgtt gttcactgtc cgggatcatt ctctcgggcc cccagtttca tgttcaatcc
    13861 cttcgcatag gctgcaaacg cggaaggaat gggatatttc tcccttgtct ccgaaggttc
    13921 aatatagatc cagtctgctg aatccgggcc ttcacgcttc ggtgccagtt catccacacg
    13981 gatcagatat cctttcatat gccattctct atgggaaaaa atatgctttg catcctcaag
    14041 aggctggatc cggagtacct gcaacccgtt ctccgccaga tattccttga cctcctccgc
    14101 agtatgatat cctttcatcg aagggaattc atacataccg gccagcagac ctttcgccgg
    14161 acgcttatgc aatgccgcca gctccgcatc ccggataatg aggatcgtct tctcctccac
    14221 cgtgcgttct tttgctttgg ctttgttcgg atattgcagc tgtgtaccgt ccgcatacgc
    14281 cagacatatc tgctgtaggg gacattcctc acagtgcgga gccccgtttg gaatacatac
    14341 acaggcaccg atttccatca tagcctggtt gaaatctccc ggcctgtctg taggaatgac
    14401 ctcccacaga tccttttcca cagccttttt tactttatcc tcagagatac agcgttcatc
    14461 cttgcggact cttgcaatga ctctcagaac attgccgtcc accgccggat tgggcttgcc
    14521 gtaagcaata gaggataccg ccccagccgt atagctgccg attcctttta attttaataa
    14581 ctcctcataa gaatccggca tgactccgcc ataatcttcc atgatctgga tggctgcttt
    14641 ctgtagattc cgtacccggt tataatatcc caggccctcc cacagcttta acagcacctc
    14701 ttccggtgcc tgcgcaagcg cagcaatatc cggcaacgtc tccataaagc gctggaaata
    14761 gggctttacc gcttccactc tggtctgctg caacatgatc tcagagaccc atacgcggta
    14821 gggagcagga tcctctctcc agggaaggat cctacggtta ttatcatacc attttaaaag
    14881 cggtttggga atcgcccgaa ggtcaccttt cctaaagatt tccttaacat ccattaaatt
    14941 ttcattgtat ttatccatag agtctaccgc agacggttgc attcgaactt ctccatggtg
    15001 agcaggcagt tcttcaattc ttcataacgc tcttccggct caccgtcatt tacttcaata
    15061 tcacaggaaa tcgccatggt cgtgatcatc tcgttggtga tacgatggtg cagtcctgcc
    15121 agaatattta gcttctcctc gtaggaatcc gcatccagaa attccagtac caacggatcc
    15181 agtgcgggct cttcatcttc tgcgggggaa accggtgcta ttgttgtctg tgccgcggtc
    15241 gccgttgcct gtactgctat gttctgcgcc acaatcggcg ttgcctgggc tgctgcgggc
    15301 tgggacgcag tcgacggcat ctttgctgtc ttcgtctgaa ctgctgccgt ctgtgtcccg
    15361 ctcactgcgt ccgcctcgcg tgcctgtctg tccgcctccg ctccctgtaa ttcaaaccga
    15421 aaacgctgtg tcacctcagg gtatctctga cgatccactt cttcggtaaa catggcaaga
    15481 ggccttgcat aggtcttgaa atctccatac aaagcctgat aaatcaccag ctgttctccg
    15541 gtctctgaat gttccgcaat cgttacgacc tgatacagat tgcccttaaa atgcttatat
    15601 atctcatgtg gtttgggaat aaatgtcatc tgtcgttctc ctttcccatc gcctcgttta
    15661 ctgtggcata tggtatcagt atacccctta tttccaaaaa agtcatgtaa atagtctcta
    15721 aaaatccgtc agcagatatt cataagagac cgaataattg ccgactgcgt tgcaggcccg
    15781 tgccagttct gcgctcaatg ctccccgttc cgtctcactc atgctgttga tcttcttatg
    15841 atgaatggta aacgtataat ccggactgcc gtcttcttcc agtgcatagg tcagcgtcac
    15901 gccgctgttg cgataaccgt tctcattcag atactgcttc atatcgctca tcagctgctg
    15961 ttcctgtatc cggttctcca tgcgctgcgt ctcatcccgg ctctcgctct tcacggttgc
    16021 gccgatacat aatacaatga tcagaataag tactgcggta gtactgtaaa atgccacatt
    16081 ggaaacgaac tgtcttctca taacccatac ctctttttct gctttttgtg ctcagggttc
    16141 tgtcggaaat cttaatttcc cgtcctgaca ggtatagatt acactttttc aagtccaata
    16201 aaactctctt atttaaaata ttcggcacct gctgcctcta ctgctgcttt cagccccagt
    16261 gcggacattc tggtggcacc gttgccccag atgatcatct gttccagtgc atcggaggtt
    16321 gccaccgtca cccggtattt ctgcggcttc gcccccagct gatgggtcgt gcgctcgata
    16381 tactggtctg cagtctccgc ttcttttgta tagaccactt ctatattatg atatttcatg
    16441 gtggagccgg gattgtcctt caccttgtag gcatcataga ctaggatcag gcgacagccc
    16501 tgatatccct gatagttaca cagaatatcc atcagtttgt ccctggcact gtccagattg
    16561 atcttcgcca gctcccgcag ttccggccag gcaaaaataa tgttataacc gtccaccaga
    16621 aggtattcct ccggtttttc ctgcggtacg accttccgca taccggtgtc accggcactt
    16681 cccgccaagc tgcccgtata gctgccacgg tttccggaag aagtatggta tctgcggaat
    16741 ctgtggggat cctccgcctt tttcccataa gtctggcgga agatctcttc gatctcttcc
    16801 tgagaaataa tccgctctac accacccccg gtctgacgaa tagcgccctg ctgcctgttt
    16861 actgcctgca gtcccgcatc cgaaatgccg tccgcatctt tggttccttc cttctccggc
    16921 ttccagccgc tctccacatg ggcatattct ggcacctggt cccaggacac ctgaaaaccg
    16981 gcaccatgag cgcagaatac cgatccggtg gggttgacca catccgcttc cggatcatag
    17041 ccgatctctg ccattacctc ctccggatta tggcagggtt catatccctt cagtgtgcag
    17101 gacaaacgcc catgaccttt ggtataagat gccacctctt ttccataatc tcccatggaa
    17161 gccacgggaa cacttcccgt cagcacactg ttattgcctt ctgtctccgg tgcttcaaag
    17221 cttccgttca tcctcgtgat atccgacatg gctctgccaa gattctccgt aggcacctcc
    17281 agtcggaagg aaaaccatgg ttctaagaga atgctctttc cgctgcgcat tccctgacgt
    17341 actgcacggt aagtggcctg tcggaaatct ccgccctctg tatgtttggt gtgggctttt
    17401 ccggcaatca gcaatatccg cagatccgtg agttcggaac cggtcagcac acccacatga
    17461 cgtttttcct ccagatgcgt caggatcagc cgctgccagt tacggtctag catgtcctcg
    17521 ctgcacagag aggcaaattg caatccactg ccccgctccc cgggctccag caacagatgt
    17581 acctccgcat aatgacgcag gggttcaaaa tgcccgatgc cttccaccgg ctccgccaga
    17641 gtttccttgt atacaatgct tcccgcatcg aattccactt ccagaccgaa acgctcccac
    17701 aggatatttt tgaggatttc catctgcacc tgtcccatga cctgcatatg gatttccctg
    17761 ttctcctcct gccacaacac atgcagctgc ggttcttcct cttccagctg gcgtagtttc
    17821 cccagtgcca cgacaggatc cacctccgga gggagcagta gtctgtaagt cagcaccggt
    17881 tccagtatcg gcgtggagtt ctcgctttca tagcccagtc cctgcccggc gaaagtcttc
    17941 gtaagtcccg tgaccgcaca gacctcaccg gctttcaccg tatccactgc ctcatagctt
    18001 ccgccattat acagacgcag ctggtcgcat ttttcctccc agatttcctc tcctgcaccg
    18061 cttctgctgt ttcccaccgg cattttaacc cgcagacttc ctccggtgac cttcaaatac
    18121 gacaatctga ttcctgcgtt atctctgccg atcttataaa cccgggctcc gaattcctcc
    18181 ggatattccg gctggggcag atacgcattc aatccgtcta acagctctgt aattccccat
    18241 tgtttcaagg cggagccgaa aaagcagggg aacagcttac gctccgccac agtgtccgat
    18301 atgttttccg ccgacagttc tccggtttcc agatattctt ccatcaaggc ttcactacac
    18361 atggctgcgt gttccagctc tgtttcccgt tctccttcat tgtccccagg ctgcagcagt
    18421 tttcccacgg atgtaatatt gctatccagc tttttctgta gctccgtcag tactttttcc
    18481 ggatccgtcc ccggttgatc catcttgttt acaaataaaa ataccggcac ttcatatctt
    18541 tttaacagcc gccacagcgt atgtacatct ccttgcactc catcgcttcc gtcgatgacc
    18601 agaaccgccg catccagtac ctgcagcgtc cgttccattt ccgcagagaa atccacatgt
    18661 cccggggtgt ctaacagtgt gatatccaca tctttccacc ggattctcgc ctgtttggag
    18721 aaaatggtga ttcctctttt ccgttccatg tccccgttat ccagaaaagc atctccgtga
    18781 tccactcttc cggccttttc tatgacacca caggcacata aaaggctttc tgccaatgtt
    18841 gtcttcccgg catccacatg tgccaggatc ccaacgatac tttttttcat gcttatccta
    18901 tccttccaat gccagcatca tcctgcaggc acgtataaaa cgttttttct attataaaga
    18961 atactatatc catgagcatt ttccaactgt tttgtatttc cagataccgt ttgcaaatcc
    19021 ggtggacagt aggatataat gtaatgagcc tcaaggaaaa acaagcggct gcgaagcaga
    19081 catttgtttt tctgggctca ttagtgaacg aatatcctgc gaagcaggat gtagagcgcg
    19141 atagcgcgga ttcgtgagct catgatataa tgagcgcaag agagcccaca tgcttgcatg
    19201 attggcgaac ggcgaagttg atcatgatag ttgcgaagca acgttaagca ccgaaggtgc
    19261 gaatcatgat ataatgaagc aaaatcactt gcgtgcaagt gtgattttgc gaaatgcatt
    19321 gagtgagctg tgctcacgat ataatgaaat gtaagattca ggaggcacta tgatcaaact
    19381 tattgcaacc gatattgacg gcacgctatt gaaggacggc actctgatgc tggatcctga
    19441 atacatgacc gtcattgaaa agctgattgc aaaaggcatc cgtttcgttg cctgttccgg
    19501 cagacaattt atcagtgagc ggaagctttt tgctcccatc cgcgacaagc tcctctatat
    19561 caccgacggc ggtaccgtgg tccgcacacc gaaggaaatt ttaaaggtgc ataccttacc
    19621 cgaagagatc tggcacggca tgtgtgagac agtccggaat gaattaccgg actgcgatta
    19681 ttttattgcc acaccggatt attgtctggc ggaagatgct ggaagccgga tgttccggtg
    19741 gcttacggat tcctacggat atgacatccg ggaatctgcc gatcttatga aaacgcccgt
    19801 ggacaatgtg atcaagttca ctgtatatca tccttccgcc tgtgaaaaaa tgtgtgcacc
    19861 tgtcttcact cccacctggc agtcccgggc gaaacttgct tctgccggaa aagaatgggt
    19921 ggactgcaat ccccttgggg taaataaggg gtctgcgatc cgttttctcc aggaatattt
    19981 ggggatcgcg ccggatgaga cctgtacctt tggagacaat ctgaacgaca tcgaaatgct
    20041 gcaaagcgct ggaatgagct atgctgtctc caatgcaaga gaggaagtca ttgccgctgc
    20101 aagggatacc tgtgcgccct attgggaaaa cggagtgttg caggtgttga aaaccttttt
    20161 ataaattccc cggggcattc cgaaagtaaa cgcttgacac tgctgcgata aagcactata
    20221 gtaaacaggt aaatagaata tccgctaggg gagcacatcg ctgagattga agtgccgcca
    20281 tcaaggcttc acttctaacc ctcactactt gaaccggata ataccggcgt aaggaagcac
    20341 attattcata cagagagttt atctgtatga atacgatgtt gtccctcctc gcgcacacga
    20401 aggaggattt tttatgttgt acactgctgt taccaatgac tggggagaga ctacttatat
    20461 ccccactacc ctgggaaatg tcctgttggt gatcattctg gccgcactgt tggcggccgc
    20521 tgttttattt gcccgcatgc agatgaaaaa gactgcggca gctcccgctt cttccgatgg
    20581 tgattccagg atgaagcacg gcaagctcac cgtgaagcag ctggcattct gtgccatggc
    20641 cattgccctg ggaaccgttc tttccaatat caaattgttc cattttccta ccggcggctc
    20701 cattactttg ctgtccatgc tggtgatctg cctgcccggc tactggttcg gtctgggtgc
    20761 cggtattgcc acaggtgtag cctacggtgt cctgcagctg ctcatagatc cctatgtatt
    20821 atatcccctg cagctggtgg tagactatct tcttgctttc ggcgctctgg gactgtccgg
    20881 tctcttctcc aatgcgaagc taggactgat caagggatat attgccgggg ttcttggaag
    20941 atatgtgttt gccgtgatct ccggatggat cttcttcggc tactatgcct gggaaggctg
    21001 ggatcccctg ccttactccc tcgcctacaa tgctatctat atcttctctg aagcagccat
    21061 aactctggta attttaagcg ctaagccggt acggaatttg tttgcgaaat tgaaagacat
    21121 ggctttgaac gactaaatag aaagttacgc gagcaatgag ccgcgcatag gcatagtgaa
    21181 gacagcgatg ggcgctcgcg catgattcgc tgtctttact tatgccgaga tgcggcgaac
    21241 gccgcgatat gaaataagat tgacagcgtt ttatgctgac aatcttatga atagcgtggc
    21301 tcattgcgga tggtagtttc aaatccac
//
LOCUS       DAWDHD010000029        20054 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_187_length_20054_cov_11.947147, whole genome shotgun sequence.
ACCESSION   DAWDHD010000029 DAWDHD010000000
VERSION     DAWDHD010000029.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 20054)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 20054)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..20054
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_187_length_20054_cov_11.947147"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         4932 a         5242 c         4264 g         5616 t
ORIGIN      
        1 ccaaagacat tccgcactcc ggggcctgcg aaaaagatct gccagcacag cgccatgggg
       61 aagttctgga cggtcttctg cagccacagg gcgatgatct ggttgccggg gtgcatgaag
      121 aggcaggtgg cgataaaact catcaccggg cacatcaggc atacgatcat ggaagagatt
      181 gctaaagtaa tgaaaacggg cttatcctcc ggggatacca tgcggaatgc cagcttccgg
      241 gacagctttt ccactgcaaa atactccaga atacaggcga tgggccacat aatgggaatt
      301 tcgtggaacg ctaacaggaa gatctcgttg gtcattcctc ctttgtccag tgcgatgttg
      361 tagcagacca ttgcgtatac cataacgaat gccatgatca gggtgaaaat aatgttctgt
      421 aatcttgttt tcggcatgaa ttaatgccct ccttttctaa aaatgggcgc aaaaagacac
      481 atgtgtgttg ttcgttaatc agtgagcagt gcgcacatgt gtcgtttcca attcttacct
      541 aaagtcttta tgctgtttta ctatttttgc aacggtgaga ttttatcaca taggtttcgg
      601 aaaagtcaat atgaaataat gtattttttc ttctgcggct tccttcggct gttcccggga
      661 tcttacgcca ttttccctgc cgctctatct tagatacaga tgggagaaaa aatgttgcaa
      721 ggtttttaaa aaagaaaaac gcaaacggga ctgtcctttc gaacagtccc tgaagagaaa
      781 aagagaaacc aaaatagttt tgggtaaaag caaattaatt ctgaacgttg aatgcgccca
      841 tgccgggata tacggctgct gcacccagtt cttcttcgat acggagcaac tggttgtatt
      901 ttgccacacg ctcggttctg ctgggagcac cggtcttgat ctgacaggtg ttcagtgcca
      961 ctgccaggtc ggcaatggtg gtatcctcgg tctcaccgga acggtgggag gaaatagcgg
     1021 tataaccggc cttgtgtgcc atcttgatgg cctccagagt ctcagatacg gatccgatct
     1081 ggttcagttt gatgaggatg gagttaccac agcccaggga gattcccttt gccagcctct
     1141 cggtgttggt tacgaagagg tcgtcgccga ctaactgtac cttgtcgccc agttctgcgg
     1201 tcagcttctt ccagccttcc cagtcttcct catccagtgc atcttctata gaaatgatgg
     1261 ggtatttctc caccagtttc ttccaatgtg cgatcagttc ttcggaggta aatacggtgc
     1321 ctgccttagg cagcttgtac tcgcccacct tgccggtctt ccactcgctg gaagcagcat
     1381 ccatagcgat cttgaaatcc ttgccgggct cataaccggc tttctttaca gcctctaaga
     1441 tggtctcgat agcttcctca tcagacttca gtgccggagc aaaaccaccc tcatcaccta
     1501 cggaagtagc caggcctctg tccttcagaa tagaagccag tgcatggaat acttcagaac
     1561 accatcttaa gcattcctta aaggaaggcg cacctacagg catgatcatg aactcctgta
     1621 catccaggcc ggaggacagt gcatgacagc cgccgttgat aatgttcatc ataggaaccg
     1681 gaagtctgtt gccggagata ccgcccagga aacgatacag gggaatatcc agggagattg
     1741 ctgctgctct cgcgctggca atggatactg ccaggatcgc attggctccc agtctggact
     1801 tatccttggt tccgtctgca tcgatcattg ccttatcaat ggcataaata tcggatacat
     1861 ccattccctg cagagtctca ttgattacag tattgatatt ttctacagcc ctctgaacac
     1921 ccttgcccag atatctgctc ttatcgccgt ctctcagttc cagtgcctcg aactcaccgg
     1981 tagatgctcc gctgggagcc atgccgcgtc cgatggtacc gtctaccaga gttacctctg
     2041 cttctacggt aggattgcct ctggaatcca gaatctctcg tccaatgact ttttcaatct
     2101 ctaaatagtt catcttgttc ttctccttta tcctctattc taaacttttt attcccaaac
     2161 cttctctttc gtgaatccct catccctgtg tataagggct gaaaaactcc tcccggaaga
     2221 tgacctcccg gcagtccgcg gattcagcag ctccgttttc tctgaggagt ttcacatgat
     2281 tcactcatta gttagttgca cctaactctt ggattattat atccggcttt cttcaaaata
     2341 gcaataggtg ttaatgagag gatttctcaa tggatatttt cctgcataat ttgtactttt
     2401 tagttaaaat taattctagt agcatttttt cattattttc ctcgatttct aaattcatat
     2461 tcctttaaaa ctaaaatata gtaaaataga atttatcaaa tcttaaaaga aaacatttaa
     2521 ataggctgca tctgcggcag aatgcgaggc attatgagga gtttcggtga aattctggca
     2581 tccctgcggg aagaacgggg catttatcag aaagaactgg ctgccatact gaaagtatcc
     2641 gttggcacca tttcgaatta cgagaacaat attcacttcc cggatcagga agctttaata
     2701 caattagcgg aatatttcga tgtaaccatc gattatctgt tgggtcggac tccttatcgt
     2761 tacaatccgg aagttctgag acaggaatgt gtccccggca ccaccgtatc cgatgtggta
     2821 gaggtgatac agcattttaa tccgaaaaac atggcttctc tgctggattt tgtggatctg
     2881 ctgcaattaa gagaaaaagc agaatcttcc tcccgggaag actctgccaa tgattgatcc
     2941 ataaattcta ttgtcttttt atctggtgga caggaactgt tgctcccgga tggctgtctg
     3001 gtcgtccggg taattttgta aataagcatt catcagtact tctgcctgct gataatcttc
     3061 cagatactca tatgctacaa tttcattaaa gcacaaggtc tgcatcaggc tgtttccttc
     3121 gatctgtttt cccgcctgaa atgccgccag agcattctga tactcctttt ttgccatttc
     3181 gcacagacca agttcattgt agatttctgc attcggcccc tgcttggaga gataattgtt
     3241 gtacacactg gacgcatagt tataatctcc ggtagccgca taggctcttc ccagatagag
     3301 atagctgtcc gcgcctccct tatcccgggc ttcatccagc gccagataag ctttctggta
     3361 ttctcccaga taataataga tccgtccgct gtcataagca tccagttgtt tggctgtgtc
     3421 cagtacattt tgcagatatt cctgaccgac ctcacggtag ccgtgcgctg ccagggcttc
     3481 atagatttct attaaccggt cataattttt cggatccatg gagattgcct gatcaaaatc
     3541 cgctttggca ctctcataat ctcccagctc cagttctgca gaacctctga gaaaataagc
     3601 gtccttttcc tccggcttca ggcccagaat ggcatcgtat acctttttgg cttccgcagg
     3661 ctgtccgttc ttggtgtatg ccgccgccag atagtaattc atatcatagt ccacgttctg
     3721 aacccagccg tcactgagtt ccagagcctg tgtaaaatat tctacggcct gatcatactc
     3781 tgtcagcccc atgctggcga ttccccggcc tcttgcgatc agacggctgt cctctttctg
     3841 tgcttccgct tcgtcaaagg cattcaatgc cccctgataa tccagagttt ccaccaactg
     3901 cattccttcg gtgatcttcg tggtatcact tccgcagcct gtcagcatgc ctgtcatcag
     3961 acagcagact cctgctgcca ctattttcct gtgtattttt cccatgcttt cctccacggc
     4021 cgtatctgta ttaccggttc ctatttgtct aactgcgaag tacaatgagg gcatcttgtg
     4081 gcatcgatag cgatctcgct cttacagaac gggcattttt tcgtcgtggg cgctgccggt
     4141 gcttcctctt tgtgatgcag ggtgtctccc agcttattga tgaattttac cagacagaag
     4201 atcacaaatg ctgtgatcag gaagttgata actgccgtga taaaattacc gtacatcaat
     4261 accggtgccg tatcgccgct tcccagcttt acatataaat tcgaaaaatc cgtgcctccg
     4321 aagatggcca gaatcggtgt caggatatct tgattcagag aagtaacgat agcggtgaat
     4381 gctccgccga cgatcacgcc gactgccata tccattacat ttcccctcaa aataaacttt
     4441 ttaaattcct gaatgaatcc gcttttcttg ctcatttttc tcttccttcc tcttatggta
     4501 tgaaggtcga gccgcgcttt ccgtcacttc gtgactacct ttgctcatga atcggcgctt
     4561 aactcatgga catattcgtt cgcaaaatca tgcaagcata attttgtctc tcgtatatgt
     4621 ccatggtcga actgttttgt tccataatag catacccccc ttgaaacctc aataggaaat
     4681 ggggtatact cttttaggaa tctaatgtag aagtaaccgc attttcccag aaaggatcaa
     4741 ttcatgcagc ggatcaacga agacatcaag aacggcaatt tcaaacagat ctatctgctc
     4801 tacggagagg agcgttatct gaaaaaccag tacacgaccc gtctgcgcaa ggctctgtgt
     4861 caggaggggg acgagatgaa cacccacttc tatcagggaa aagatttttc tgtgggagag
     4921 gtcattgatc tggcagagac attgcctttt ctggcagaac gccgggtcat gttcttcaag
     4981 gatacagggc tttttaaatc cggcggcgaa aaactggcgg aatacctggc aagccctaat
     5041 gacaccactt tttttgtgtt cacggaaagc gaagtggata aacggagtaa actgtataag
     5101 accgtgcaat ccaagggtta tgtggcagaa ttcacggtac aggatgagaa ttccctgaaa
     5161 cgctggattg ccggtatcct gggccgggaa gggaaaaaaa tctccgagaa caccgcacag
     5221 ttgctgctct ccaaaaccgg cacggacatg gaaaatatcc agatggaact ggaaaagctg
     5281 atctgctact gtctggaccg ggatgtagtc accgcagagg atgtggaagc ggtctgcacc
     5341 actcgcatca ccaaccacat cttcgatatg gtcaatgcca ttgcggaaaa gcagccgcag
     5401 aaagccttgc agctctatta cgatctgctg gcactgaagg agccccccat gcgggtgctg
     5461 ttcctgatcg ccaggcagtg taacctgctg ctccagacca aagaattaaa aaacagaggc
     5521 cacgacaata agaccattgc ctccaagatt ggtgtaccgc cctttgccgc cggaaaatat
     5581 gtggcacagg catcccattt caagacttcc gtgctgaaaa atgcggtaaa gcagtgcgtg
     5641 gagactgagg aagccgtgaa atccggacgt atgaatgata tgatgagtgt agagatactg
     5701 attttgtcgg tattgccctc gtaagcttgc gaaatgcatt gagtgagttc tgctcacgat
     5761 atagaaaagt ccatggacag tcttcctgct catggacttt ttatttgcat tacttttatt
     5821 accatgttat atattcagat tcaaagtaat ggtcttctgc aatgatacct gtgacgcctt
     5881 gaactttttt ggatatacat ctttgtatct gtaaatactc agaataattc ctaccaatgc
     5941 ataagacagt aaaatattat ctctgccaac agataagaac ggcaggaatg ttgtcgtcag
     6001 cgggatccat ccaaaaattc cggcaagatt caggagaaca tttaataaaa tgatcattcc
     6061 gcaaccgaat cccatcactg tgccaagttc atttttctgt ctgacagaag ttccgaacat
     6121 aaacactacc aatgccgcca ggagaacgac cacaacaatt cccgctgact ttccataact
     6181 attcagaata taggaaaaaa cgtaagcttt attaaattcc ggcagacctt cgagcacatc
     6241 atttccacta tttccccaaa acgaaatgtt ctcattaaat ttatgaagca tggacgtcat
     6301 ataataggca tctccggaag aggttaaata agagcggatc cttgcttcct gatatactgc
     6361 cagcatatga aatgcgtaca ttgcagccag taacagaacc ggtaatcccg caagaaacaa
     6421 ccccaagcca ataatcgtct ttttgacggg cagtttaaac caccccttcc aaatagccac
     6481 cgtaagttcc atcagcatac tgatcatcat gatgacagcc acaccgaggc ttggaatccg
     6541 gaatgtaata aatactggaa ggatcagcca caggagtgct ttgcacagtg ccataaaccc
     6601 accatccctg tatttgtata agatagctcc atagatgggc acatagaaca tcatcaggga
     6661 agtaatcgac acccggaaca ttccaaatcc aacccagttg ccgactccgt taatatccac
     6721 accgaaaaag ccggtcagcc gcaatcctcc caaaataagt ataaagactc caatgaacct
     6781 ggagtacttt gcaattaacg tataatccag aaagtagatc acacacatga gaaggaatcc
     6841 tatcacaata catgagacaa acccctccgt agtatagcgg tacacttcct gtctacatgt
     6901 ttccagctcc tgataaccgg gttgtctgag aatggattgc tgtatcagaa tgcccagcag
     6961 gcttaaaatt cctacgatca caagtaacct ccatgcaatc ttaggcttgt ggatcctgtc
     7021 cagcgagatc cccacttcta ccggatctcc catgtccgta accgcatttt tctctgcttc
     7081 ctcatagctc attccctcgg aaagattgtc tgcgatctgg cattctatat ggtctcttat
     7141 ctcctcagcg atatagggtc tggctttttt acagcggatc tgtgacaaca gtttttccaa
     7201 atatgtctcc atccctatac ccccattgcc agaacactgg tcactgcaga ctgatattcc
     7261 atccactccg cttttttggt ttccagtaat tttctgcctt ccttcgtaat actgtaatac
     7321 ttcctcgtct tgcctgcata ttcctgctca tataccgtaa gcaggccctt cgcttccagc
     7381 ccatgcaaca gcggatacag cgtacctgct tttaactcga acacgttaca ggattttttc
     7441 cgcagtgtat cgatcatctc gtatccgtac atatccttct ccgccagtaa tctgagcagg
     7501 agcatcgtca tacttcccga catcagtgat ttgtccacag ccattttttg tcctccttat
     7561 atagatagcc gatatatatc gttgtctata atatatatcg gctatctata tatgtcaata
     7621 tttatttttc aaagcaatca ctaatccatc tcaacttcat ctacgatttt gtatttgtta
     7681 tccgtatctt cttttcttat aataaacagc acatccgtag tcatctcgtt ctgtctaaca
     7741 gcgttccccg aatcatcaaa tcattatttg ctgtattttt ctacattttc aatgctttcc
     7801 gataccgttg cattctccgg ctcccattca cggatctggc agctggcaac catctgcgag
     7861 ataatctttt tatatttttt ataatccttt tgtgacatag agatggtaat attatagaat
     7921 ccctgaccgg tgaaatcccc tttaaaaaca taggattcgc ttatccattc accgcttgtt
     7981 tcgattccat gaagcgccgt aagttcctca ttcacttcca cggtctccag atcattttca
     8041 tcccctgcat aataaggaga aaaactgtta caaaaataga tactgctctc ctggctatca
     8101 tccgtataaa tccggattcc ctcgtcggga ctggcttcct ctgtctcggt cgcaggccac
     8161 ccttcctgaa gcttcgctgt ccattctaca ggtctgtcta tgcttaaggt aaaccctgct
     8221 gtctcatttg tgtattcgca gcggatcatc tcctgctttc cacaaccggt cattgcccat
     8281 aatgctgcta aaatcatcag accctcaatc cattttttac ccataagcac ctccgtcatt
     8341 ctactctgct gttgctatct ctacaccctg ggcaatatct tccccccagg atttttcctc
     8401 cctgcaatag cggaactcct tcgcattcag gtcaattgct cctaattctt cctgtcccgc
     8461 aatatataag tcatccctaa tgcggtaacc cctcggtccc cataagtaac ctcctaatat
     8521 tccggtcaga ataaatacca taaaatagag tcccggaaca cctgcgacaa aagcataaaa
     8581 cgctatctgg aagctccaga tggggattaa cagattgagc gctgccacac aggcaacgcc
     8641 aaaagcaaca tattccgcac cttttgcttt gtcccgtctg gtccttgtcg ccaacaggaa
     8701 tccccatgcc gtatagagca gcatacgtac caccgtccgg actccgctca tttcgtcaaa
     8761 agaagttttg taatcctcct ttagtgccac caggacaatc aggatcacaa cagacaaaat
     8821 aattttgtag taatttgaaa aaatcttctt catagctgct cctttcattc tacattcagg
     8881 aaagtatatc ctcccatatc cgtatccttc cacgccaggg cgtcttcctt gttcctgtag
     8941 tatacagtct tgacaacatt ggtacccagt tcttcccggt ccaccgtttc tctcacttca
     9001 cgcaaatgct cattaagtct ctctgccgtt acttcccctt ttaccacata cgaaatttca
     9061 gatacattcc cccagtcagg atcatcgatg tatcttgtgc ctatctgctg ataagtaggt
     9121 tctctatgct ttatcatata tcccaacagc agtcctccga cgatcaccag tattcccacc
     9181 aggacagcag tttttactat aaggctgttc tttttcttcg ggggctctcc gtccgtttcc
     9241 tctgtctctc ctttctgcgg ttctggattc tgcaattcat cacaggatat gtctttgctc
     9301 ctcaattgta cttgctaatg acataattat acaattgagc aattggaaaa tcaattgagc
     9361 gaacggcaac cttcctcttt gttaatcata acgaagaatt tttcgctatt tgttacaaat
     9421 ttcaaaaaaa gcgtgcagat ctctccacac gcttttcgtt catcgtctta tttattcaaa
     9481 aattatttcc acattcccgt cgcaaagcac atcaatgccg cccaggtata taacgggtaa
     9541 aaaaatccct ggtcatccac gccgtctttt aagattttct gtgcttcttc tttggtcagc
     9601 agcagaaaac catcaaaaca ttccgttcca acggcgcctt cctgacttac cttcggcatc
     9661 tcttcacggt tcaatatgat ctgcaccagt gcattgctct catccgtcat accgggggta
     9721 ctgaatacca gcggattcac cagacggatg ctgtcgctct cttccagtgt gatgccagtc
     9781 tcttcccgaa gctcccgggc tgccgcatgg aatacgggat tttcttccgt tgcatcctcc
     9841 gggtctaaaa gtcctgccgg aacgctcagc agaaaatgtc ctgccgggta acgatactca
     9901 cggcttaagc aaagtctcgg ttcttgtcct ctgatcttca ggatcacaac gcagctgact
     9961 gcatccggca gcatctgtct gaattcttcg tcgctcttta ccgctgccag attatctttg
    10021 gctcttctgg tagcatcaaa ataatgtttt cccggctcat attgcagatc aaataccttg
    10081 ataaatctgc tgtcgtagac ggtctctacc tgttcctttt ttatgatcgg tctgttctcc
    10141 atagtcattt tcctctttct gttcttctgt tccgcacgtt gcacactatc tgtcgttcgc
    10201 ttctgttgtg cggctgtcat tttgcaattt ttccgcttcc ccggccgctc tttccagtct
    10261 gcaaagtacc tgttcgtaac gtcgtcccgc ttttttccag tggatcagct tatgccatcc
    10321 ttccacgtaa tccggcctgc ggttctgata ctgtaaataa taggcatgct cccacacatc
    10381 tatgggaaat atagaggtat acaatttcag atccgggaca tcctgattcg ctgtagtaag
    10441 gaccatcaga gtaccgtcct ggtcactcac aagccatgcc catccggagc caaatacccc
    10501 cgtggctgcc tgtgacatct gttctttcca ttgtctcacc gatccaaaat cccggaccag
    10561 cgcctcctcc agagctccac agggctcctg tcctgccggg cttcgcatac tgtcaaaata
    10621 cagctgatga ttatatacac cgccaccgtt attgtgaata cttttccggg cttcctccgg
    10681 cagggacgca agtatattat cctccgaagt gagcagttcc gtcaaggaca tctgctgtaa
    10741 ctgcggataa tcggcaaggg tggcattcag cttatccaca taggtctgat aatgcttgtc
    10801 gtgatgaaaa tgaagtgttt cctcatctaa taccggaagc agggcatcat attcgtaggg
    10861 aaggggctgc gcaacaaaag gataatgttc ttctaacatg agactccaat ccgcctgcgg
    10921 gatacaggct tacgatcata caatatttac catacgccta gtatatgctg tccccacgga
    10981 ttttattttc aaatttgtgc ttttacagcc gaccgctcct atgctctctt tctctgcacg
    11041 gccggataga tctctctggc aacttccaga ctttgtccgg atctaacttt ctgcacatcc
    11101 gctgtctcct gggctaacag ccatccacag acttcctctg ccagtggctc tccctgttct
    11161 ctcagccaga agctgacatt cgccagcatc tgtaaatgat tcaggatcgt attacggcaa
    11221 agtcttcggt ccgtaatgcg ggtcttacag ggctccccct ctacgtacag ttgtgccgcc
    11281 tcccgcagat aggcccggat caatgcggtc tgttcatgat tgctcttctg cagttcctca
    11341 ctgatcttct tggtctccca atacagttct tcgctgcatc ggccagcggg aacgatccct
    11401 tccatgtatt tttgtataaa atgcaggctt tcatttacct gtctgcaata ttctctgatc
    11461 agttcttctc tttttgacat aataaaaaac cgcccttaaa ttaaaattta agggcggaac
    11521 attcttccgc ggtaccacct taattcgccc atctctcacg ggatcaggcc tctgcaacta
    11581 cggataaaca aacgctcatc gatagtcagg tacgataacg ggtactcccg ttgcagcctg
    11641 tccgcatctg ccggatatcc tgtccgaata cgaagtcggt gcaaagctcc gagaccatgt
    11701 tcaaagaagc cttccatatc ccttcccacc tgccgggact ctctgcgacg tatctcttct
    11761 cctactcttc tcttcacagc ctttatgtct ttaactgttt gtattttatt cctttcgcgg
    11821 acatttgtca acactttgtt tttgtcacaa acacaaacag tggagcaact ttcgcgactc
    11881 cactgttctt atccattttt atatcttaca gttgtgcaat atccaccagc gtcagttcct
    11941 tacaatcgtt catatgctcc atgctggtca cgagagcatt ggcggtgtcc attgcagtga
    12001 tgcagtagat accggtctcg atagcggtac ggcggatcag gaaaccgtct ctggtcttgt
    12061 cgcggccctg ggtcggggta tcgatgacaa ggtcgatctt gtgtcccagg atcaggtcca
    12121 taacggtcgg ggattcctga gagatcttgt tgacctttct tgctttcaca cctgcttcat
    12181 tcagagcagc tgcggtactt ctggtagcat aaatggtgta tcccagcttt tcgaagcgtc
    12241 ttccgacttc aatggcttca cccttatccg catccttcac ggtgatgatc atgttcttgt
    12301 acttaggcag ttctacacct gctcccagga atgccttgta cagagcctca ttgaaggtct
    12361 tggcgatacc cagacactca ccggtagact tcatctccgg tcctaagctg atctcggcac
    12421 cacggatctt ctcgaaggag aacacgggca tcttgatggc aaaatactct gcctcaggct
    12481 gtaagccggg ctcgtagcca agctcacgga tggtcttgcc gatgatcact tcggtcgcca
    12541 gatccacgat aggaataccg gttaccttac tgatataagg tacggtacgg gaagagcggg
    12601 ggttaacctc aatgacatat acttcctcac cgatggcaat gaactggata ttgatcagtc
    12661 ccttgacatg cagtgccttt gccagtcttc tggtgtactc tgcgatcttc tcttttacta
    12721 ctttatcaat ggtcggagcc gggtatacgg agatactgtc tccggagtgg ataccggttc
    12781 tctcgatatg ctccatgata ccggggatca ggatatcggt accgtcgcat acggcatcga
    12841 cctcgatctc cttaccctgt aaatacttat ctaccaggat cgggtgatcc tgtgcgatac
    12901 ggttgatgat ctccatgaat tcttcgatct cttcatcgga gatagcgatc tgcatgccct
    12961 gtccgcccaa tacataggaa ggacgcacca gtacgggata gcccagtctg ttagcaaccg
    13021 ccttagcttc ctctgcggtg tatacagtac cgccggctgc acgggggatc ccggtctctt
    13081 ccagaatctt gtcgaacagt tctctgtcct cagcggcatc tacatcctcc gccttggtac
    13141 ctaagatagg cacgcccatc ttcatcaggc tctcagtcag cttgatagcg gtctgtccgc
    13201 cgaactgtac cacagcacca tcgggatgct caatatttac gatagattcc acatcttccg
    13261 gagtcaaagg ctcgaaatac agcttgtccg caatatcgaa gtcggtactt acggtctccg
    13321 ggttattatt gatgataatg gtctcatagc ctgccttgga gaatgcccag gtagcatgta
    13381 cggaacaata gtcgaactcg ataccctgtc cgatacggat aggaccggaa cccagtacca
    13441 ggactttctt gcgtccggtg gtctccttgg cttcgcactc gccgccaaat acggagtaat
    13501 aataaggagt ggaagctgcg aactctgcgg cacaggtgtc taccatcttg taaacagcct
    13561 taatgccgtt gtcataacgc atcttcttga tctcttcctc ggtcttgccg gtcagtcttg
    13621 cgatgacgtt atccgggaac tcgattctct tggcttcctt cagcagttcc actgtcagag
    13681 gctgtgtctc cagtgcatgc tccatctcgg tcagaatggc gatcttgtca atgaaccaca
    13741 ggtcgatctt ggtaatgtcg tggatctgtt cataactgat acccttacgg attgcttcag
    13801 cgatcacata gattctctgg tcatccacga tcttcagacg ctctaacagt tcctccacag
    13861 acaaagcgct gaaatcatag ctgcgcaggc agtccacatg ctgctccagg gaacggatgg
    13921 ccttcatcag tgctccctcg aagttattgc agatactcat aacctcaccg gttgctttca
    13981 tctgggtggt cagagtacgt tttgcagtga taaatttatc aaaaggcagt ctgggaatct
    14041 tcacgacaca gtagtccagt gtcggctcga agctggcata ggtcttgtgg gtaatggcat
    14101 ttttgatctc atccagagta aagcccagtg cgatcttcgc tgctaccttt gcaatggggt
    14161 aaccggtcgc cttacttgcc agtgcggaag aacggctgac acggggattc acctcgataa
    14221 cacaatattc aaagctggtg ggatgcagtg caaactgtac gttacatcca ccggtgatct
    14281 gcagttcgtt gatgatcttc agtgcggaag tacgtaacat ctggtactct ttatcgctca
    14341 gggtctggga aggagctact acgatactgt ctccggtatg cacgccgaca ggatcgatgt
    14401 tctccatgtt acatacagtg atgcagttgc ctgcgctgtc acgcattact tcgtactcga
    14461 tctctttcca gcccgcgata caacgctcta ccagtacctg tcctacacgg gacagacgta
    14521 aaccgttctc caggatctct tccagttcta cctgattgtg ggcgataccg ccgccggaac
    14581 ctcccagggt ataagcggga cgcagtacaa ccggatagcc gatggtattg gtgaattcca
    14641 caccgtcttc cacattttca acgaccctgg atgctgcaca gggctcaccg atgcgctcca
    14701 tcaggtcttt gaactcctga cgtccttcgg cgttacgaat ggtagctgcg gtagtaccta
    14761 acagctttac attatgagca gccaggaaac cggactcctc cagctccata ccgaggttca
    14821 gaccagcctg tcctcccagg gtgggaagga tgctgtcggg cttttccttt tcaatgatct
    14881 gctccagaac ctttacgttc aggggctcga tatatacctt atctgcgata tctttatcag
    14941 tcatgatggt cgcagggttg gagttcacca ggattacctc tactccctct tccttcaggg
    15001 aacggcaggc ctgggtacct gcatagtcga attccgctgc ctgtccgatg acaataggac
    15061 cggagccgat taccattact cgttttacat taggattctt gggcatctta gttctctcct
    15121 ctcatcatat gaatgaaccg gtcaaacagg taaccggagt cctggggtcc cggacatgct
    15181 tccgggtgga actgtacggt gaaaatgttc ttgccggtgt aggacagacc ctcgttggta
    15241 ccgtcgttga cattgataaa tgcgggaacc gctacggaag gatccagctt atcggtatct
    15301 accacatatc catggttctg agaagaaata tatactcttc cggtcatcag atcctttacc
    15361 ggatggttgc ctcctctgtg tccatatttc attttgtggg tatctgcgcc ggtagccaga
    15421 gccatcagct gatgtcccag acagattgcg aaaataggga tctcggtatc gtataacttt
    15481 ttgatctcgg cgatcacacc ggtacactcc ttggggtctc ccggtccgtt acttaacatg
    15541 ataccgtcgg gattatcatc aatgatctcc tgtgcgggag ttccagcggg gtatacggtc
    15601 acctcacagc ctctctctgc cagggatctg gcgatatttc tcttggctcc caggtctaac
    15661 agagctacct ttagtccctg tccggacagc tccgttacca gtgcgggctt gggctctctg
    15721 acaccctttt cataagcttc tttatcaaaa cgcgctgcac cggacagagg gccgttctgt
    15781 gccagatccg tggtgggatt cactacatac ttctccttgc aggtcacctt ttccacgacc
    15841 ttgcctgtgg tatatgcttt cagcttcgga agcacttcgt tcaggtcata ggcttcgttg
    15901 gtggtgatca taccgttcat ggtacccttc tcacgcagga tcttggtcag cgcacgggta
    15961 tcgattccgg cgataccggg cacaccgttc tcctctaagt attcctgtat tgtgcagtca
    16021 cagcggaagt tgctgggaat acgtgaaagc tctctgacga tgaatccatc cggccagggt
    16081 ctctccgact cgcagtcatc cctgcatatg ccatagttac cgatcagggg gtaagtcatg
    16141 catacagcct gtcctgcata ggacggatcc gtcaggacct ccagatatcc ggccatggat
    16201 gtgttaaata cgatttcact gatgatctct ttgtctgcac cgatgtgcgt tccttcgaac
    16261 accgttccat cttccaaaat caaaaatgcc ttcattcttt cctcacattc tgccgctttt
    16321 tgcgacgttt gtatttccag tgtgtttctc tactgcttaa tcgattcatt atatcataaa
    16381 aaaaaatgcg tcgcaataga ccctgtctgc gtaaaaacct acaaaaaaca attacgcaat
    16441 cccaatactc cttggagaat ctgacaaagc tgtgtaaaag cttacatttt ccattgcttt
    16501 ttccaccggg tctgatatag aatagatata tattttctat ttttcaatta tcacaaaacg
    16561 ttgttttcat acagcagatt ggacaggcat ctatggatcg tatcgaaaat gctcaaatga
    16621 actcccaggg aacccgcaaa ctctattatg aaaatgtgca ccagacggat tttaccgctg
    16681 tggtgatgga atgtgttccc gacaaggaag agggatatta caggatcgtt ctggacgctt
    16741 ccgctttttt cccggaagag ggcggtcagt ccgccgacaa aggcacctta aacggacagg
    16801 aagtactgga tgtcagcatc aagcagggca tcctgtatca taaagtggcc tgtgagtttc
    16861 ccgtcagcac ccgggtcacc ggtcatgtgg actggaaccg ccggtttgat ttcatgcagc
    16921 agcactccgg tgagcatatg atctccggct tactgcactc tcatttcgga ctggaaaacg
    16981 taggcttcca tttaagcgac agagaagtga cactggatat gaacggagaa gtctctctgg
    17041 agcagctgcg tctcatcgaa caggaggcca acgcttatgt ctggaaaaat cttccggtca
    17101 acatcagctg gccctcctcc gaagaactgt taagcttaaa ctaccgcagc aagctggctc
    17161 tcacggaaaa tgtacgtatc gtcgagatcc ccggcgtgga tctgtgtgcc tgctgtgctc
    17221 cgcatgtaga taccaccgga cagatcggtc tcatcaaagt tgtaaatgtg cagagccaca
    17281 gaggcggtgt ccgcatcaat attctctgcg gaaaccgtgc tctggcagat tatactgaaa
    17341 aacaggattc tgtctgggca atttcttctc tgctctcagc gaaacagccg gaagtagcag
    17401 gtgctgttgc ccgcgccaaa gaggatgcaa ggctgcaaaa agagcgcgcc aatgccctgc
    17461 aggcagagct actgaacgta cagatggacg cacttccctc cccggaaaaa gtacgccacg
    17521 tgcttctctt cgtcggtgaa ctggatgcca tcgccctacg aaacgctgtg aataccctga
    17581 ccggaaaata ttccggttac tgtggtatct ttgccggaga tgatacaaac ggttatcgat
    17641 ttattgtagg cagtgcttcc tgtaactgtc aggaactggc agaccgcatg cgccgggaac
    17701 tttctgcaaa aggtggcgga tccgctccca tgatccaggg gaatgttgct gtcacggcag
    17761 ataccctgca gacaatgtgg gtgtcgctgt aatgaccata ctttctgcct atatatgcat
    17821 ttcggcacat gaattccgta ttcaaaattt ccacatggaa aacccccggc atgaccatct
    17881 gtcatactcc gggggtctgt ttgtatattt tatatatttt tcgtttcggg aaatctgatt
    17941 atttctcttc tgttgctgca ttcttcttca gtgctacgct gactgccttc gcaacgccac
    18001 cacctactac acagctcata ggcatctcca gtgctccgtt gataccacag aatgctacgg
    18061 cgaacataaa cagattcgct ccggttgcat tcatgctctg cagatactcg gtatgaccga
    18121 agcagatcat cagagtagac atgaagaaca gggtgttcat aaatgctccc agcagaccgg
    18181 tcacgaagta agttacggtc ttcgtcttgt caatcttcca cagtgccttg aagatcacgc
    18241 cggacagcca tcccatcaga gttctggtag ggatgcatac caggaaagtg aaaaacggat
    18301 tcattgctac caaagctgct ccaaaagggt ctcctgcaaa acactggtaa aagctagtaa
    18361 gtccaaatac cagtcccagt acggctccgc ctgcgggtcc taagatcatt gcaccgatta
    18421 ctacgggaat ggtcatcagg gagattgcta acggtccgat gcgcagatac ccaagcgggg
    18481 taaacgccag aatcagcatg atcgcgatga gtacggccaa ttgtaccatc tcaagtgtct
    18541 tgtttttttt catatgaaat ttcctttccg cgctttttgc gcctttgcta ctattcctcc
    18601 atctggcgtc ctttggactg ccggaggata taattacaag tcattcaaga aaataacata
    18661 aatgtataca aaacacaagt acatttttta atacaatcaa cgtacggtgc gataccactt
    18721 caacgccgat gtataggctt catatactgt attcatatca atattcttta atagcatcag
    18781 gcgggatacc tccgcccagt tggctgtctc atactgcagc ataaaatcgt acaccggtgc
    18841 cagtacgccg atgcggtaga tcagtgcatt gctgatctcg ccagccacat ggaccacctt
    18901 caaagcctct gccatgggtt tctcgaggat cacatccagc acggagaaca atcccatcag
    18961 gaacaattca tccttctgcg ccttcaggcc aaacgcttcc gccagattct cggcaaattt
    19021 cgcacgaagc agagaaagtc tggtaacctc attcggctta tccgcataca aagcattggc
    19081 tactgccgtg ttgatccact ttttcagttc ccgctgtccc agcatggcag ccgcatgacg
    19141 gatagatgta atctccgaat ttacagccag tggctgaacc attttcagca gatcgatcgt
    19201 aagtgcggta tcacggctga tgatatccgc cgctgtcgtg agttcaaaat ccgggctgtt
    19261 cacaatattc agcaggtcaa tatagttacc cttcaagggt gccacatccg tctgtccctt
    19321 cgtaatggga actcgataga acttaccctc atagaaacga tatccgccgg tctccttcag
    19381 tctctcgaag tcctccatgg tatcaatatt accggcacac agactgatat tcgggaataa
    19441 ttttccgaaa taaatcttgg ctttgtcaat ggcgatttta cgattattca gaagaacata
    19501 atccatcagc ttcaggactt ctctgtaatt ttcaaaatca ctgaccgcaa gcttgcgaat
    19561 ggcaagttta tagccttgct gtttcaattc cttcagacga ttgacataca tttcaatcgg
    19621 cggaatggta ttatcgatca aaagtacgat tttttcatga ggtgcatcgc actgctccgg
    19681 aatatccgca aaaatcgaga tattgctgat cggtacgaag atttcttttc cctgagacaa
    19741 ggtatcaata cccattttct ggatcagttc cagacccgtg atctgggatg ccccgtcgaa
    19801 ctgtgcggtt ccaagcaaca gaggattcaa caggaaattc tccctctggg taaaaaagga
    19861 gtaggcacaa acggccatat tttcatcaaa aagcggtact aatgtaagca gcatattttt
    19921 acctcccaat ctatctatat tataccgatt ccgacaagac tttacaagtg gtactatcag
    19981 cacagagatg taaatagaga gttagtaaag aaatgctatc aaatccccgt agcccttggc
    20041 agggtataag aaac
//
LOCUS       DAWDHD010000030        19827 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_189_length_19827_cov_10.964950, whole genome shotgun sequence.
ACCESSION   DAWDHD010000030 DAWDHD010000000
VERSION     DAWDHD010000030.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 19827)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 19827)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..19827
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_189_length_19827_cov_10.964950"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         4451 a         5349 c         4021 g         6006 t
ORIGIN      
        1 cctgtacatt ccggaagcga tcagcatcag cataaaagaa tactgataca agacaccggt
       61 aatgctggtg gtctctttcg tagccggatc cagtaacgta gccatggaca ggcctgtctc
      121 catgtcagca atggaaccgg caaaattcac gactgccata cagatattgg ccccgaaacc
      181 gatcagcagc ccggcaattg cctctttcat gacgatcacc gcatattcca tcacagtgtc
      241 aacaactacc gcatccgccg gtgtcagcgt ctcataaagc agccacgccg taaaaaagct
      301 gatgccgatc cggattctgg ccggggtgtc attcatcccg aaataaggcg ctatatatac
      361 aaagcaggat actcttacga atatcagcag aaaatattca agatcatata ttgaaaaaga
      421 aagatctacc atatttcttc ccgtcaccta tgtacatact ggctgaaact tgaccacaat
      481 tgcaccgtgt actccaccat cgtgttcatc atccagtgac cgataaccac cagacacaaa
      541 aagatggcaa cgatcttcgg tacaaaggtc agtgtctgct cctgaatgga agtcaccgtc
      601 tggaaaatac tgatgaccag accaacaacc agggatacca gcaacaccgg cagggatacc
      661 ttgatgatca catataacgc attattcgtc atttccgata ctgcatctat cgtcatagca
      721 aaacctcatc ttacgaatct aataaaatgt ctgtaccaac tgccctatga tcagtgccca
      781 gccgtctgcc agtacaaaca gcagaatctt gaaaggcatg gagatcgtcg tcggcggcag
      841 catcatcata cccatactca ttaaggtgga tgccactacc atatcgatca caataaacgg
      901 tatatagatc ataaaaccga tccagaacgc cattcgcagt tcactgatca taaagctggg
      961 aaccaacacg gaattcggaa tatcctccag ggttccgtcc cactcctgtc ctgcgatatc
     1021 catgaacagt tccacatcct tcacctgagt ctgtggatac ataaattccc gtaagggtgc
     1081 catgcctttt tccagagcct catcctgatc gatcgtgccc tcctcaaaag gctggattgc
     1141 ttcctcattg atcctcgtga tcgtaggctc cataatgaaa aatgtcagga tcagtgccag
     1201 tccgatcagg atctggttcg gcggtgccgt ctgggtattc agtgcagacc tggtaaaatg
     1261 cagcacgatg atgatcctcg taaaagaggt catcatgata ataatggtcg gtgccagtgc
     1321 gatcagcgtc agagtgatca gaatccgaag cgcaccgttg atctgtccgt taccgttatt
     1381 gtaggtaatg ctgacagtat tcgccgtatt cagctgtcct acctcttccg gggtcgtggc
     1441 tgttcccgcc ggattgatca ccgtggaaac ttccgtatct cccgtcagat gacgctccga
     1501 tgcctgcacc tttaccggcg tacaaataca caatatgcct accgtgacca gcaggcatac
     1561 catatagata atcgtcttta attttatact tgcatcaatg atctttctct tcattctctt
     1621 tcttcgccgc acgttccaat aattcccgga acccggaagt actttccaat gtagtcttac
     1681 ctttcagttg ttctgcagga atctgtccca gggccgttac ggtatccttg cagaccgcaa
     1741 tggcaaaata agactcaccg atccggacca gctgaatgta tttgttattt gcgatccgaa
     1801 cggtctctct cacttcaata ttgcctgccg ctccctgttg tttctggtat cctgcgatcc
     1861 atttcgttac caatgcggtt accgccagta ctgccacaaa gatgaacaat actgtaatga
     1921 actgggcata actgtctgct tttccggtaa gtatgatcat taacccacta cctttttcac
     1981 agcttcaata acacgttctg cctgaaaagg ttttacaatg aaatctttgg caccggcctg
     2041 gatagactcg ataaccattg cctgctggcc cattgcagaa cacatgataa ccttagcacc
     2101 accgtctaac ttcttgattt ccttcagtgc ctggatgccg tccatctcag gcatggtgat
     2161 gtccatcaga accagatcgg gattcacttc cttgaacttc tccactgcct tcattccgtt
     2221 ctctgcttct cctgcaacat tgtaaccgtt cttggtcaga atgtccttaa tcatcattct
     2281 catgaacgct gcatcatcgc agatcaaaat attctttgcc atttaatttt cctccatttg
     2341 tttggtctat tctctgtttt atttaataat ttccgtaata cggacgccaa agctttcttc
     2401 aattacaaca acttcaccct tggctacaaa ttttccgttt accagaacat cgatcggttc
     2461 tcctgcaata cgatccagtt caatgatcgt acccggtgag aactccagaa tctcggaaat
     2521 ggatttcgtc gttctgccaa gctctacggt aacttccaaa gggacatctt tgatcagacc
     2581 aatattttcc tgtccggaaa gtcccatgct gtcatttgag aagctctgga actgtgcagg
     2641 ctgcacattg acattctgct gcggcattcc catattgccc atctggttca tcatgcccat
     2701 gccattcatc tgttgcattc ccatgttccc catctgctgc attcccatct gaggcatacc
     2761 attcatcccc atctggttca tgctattcat gtccatgccg ggcatctgat tcatacccat
     2821 gccacccgtc tggttcatgc cgttcatctg tggcattccc atgttgccca tctgattcat
     2881 gccattcata tgattcatag ccatgggatc cggttgctgc atattcatct gagtcatatt
     2941 gtttgcgccg gcattcatct gtcctgcatt gttctgcgga gctgctgcgg gagcaggtat
     3001 gctttccggc tgagttgctg cctgatcgtt gctttctccc aggcttccca tctgggtatt
     3061 gataaaggtc tctaccagct tacgggcaaa atcgatggga tacaactgca tgatagaact
     3121 gtctaccaga tcatcgatct gcatacggaa tgcgattttt acaaaggttc ctcctaaaaa
     3181 cggagatatt tccttgccat cctttacctc tgtcagatca aaaagagaag actcaggcgg
     3241 gctgatatca attaccgtct gcaacagtgt ggaaagggaa gtcgctgcgg agcccatcat
     3301 ctggttcatc gcctcggaaa ttgcactcag atgcagttct cccagctctc catctgtatt
     3361 cgtaccatca ccgcccatca tcaggtcagt gataactttt acatcatgtt cttttaatac
     3421 cagaatattg gtaccgtcca gtccctgggt atatttgatc tgaataaata cacagggctt
     3481 ttcataggcg tccagcaggt ttttccatgt agccagcgta accacaggag ttgtaatatt
     3541 tactttgcgg tttaccagtg aatatagggt tgtggcagag gatcccatac tgatattggc
     3601 cacctcgcca atcgcatcct tctctacatc cgtcagttcc tctccgtcac tgacaaccgg
     3661 tgctgcgttc tccgcagctt cattctcatt attataattt tcagccgtac tgttctccgg
     3721 tgccggatct gctgccgcat caccgccatc cgcggtatcc gacagatcca taccttgcag
     3781 cagggcattt atctcatcct gagataacat cccatccatc gttactcctc ctctctgatc
     3841 accgatgtga tctgaacagc atacgaatct ctatccgtac cgggcagtgc cgtgaacttt
     3901 ttaatattgc ccacatacac attcatgtcg gtatcgactc tggaatccag ccggatacag
     3961 tcgcccacct gcagattcag gaaatcattc accgaaatgg tgctccttcc cagcactgcc
     4021 ttgatcggaa tatctactct ccggatcatg gactcaatgt actcctcgta gtgttcatcg
     4081 tgattctcct gcattgtgga gaaccagtat ttggtgttca gtttatccat cacatcttcc
     4141 agtgtaaaga aaggaagaca gatatttacc attccttcga catccccgat tttcatattc
     4201 agggtaacaa tagcgatcat atcgctggga gcaattacct gtgcgaactg gggattcgtt
     4261 tccagccggc ttaacaccgg tgagatctca acaacgtttt tccagggttc acgcatcagc
     4321 tgcgtaaaca tgaccaaaag cttctgtaag attgtcagct ctatatcgga aaaatctctg
     4381 ctcttttcca atggctggcc gctgcctccc agcatacgat ccagcatggc ataacataga
     4441 ttagttgcaa tctctataat aatattgccg ttgagtggtg caaaattaac aattccaagc
     4501 accgacggat tcgaaagtgc gttggagaac tcactgaacg taaccgtttc cgaacttgct
     4561 accgatacct gcacgttctt gcgcagatat accggaaggt tcgtagagat cagtcggctg
     4621 taatgctcga atatgatctc caaggttcgt aaatgttctt tggagaattt ggtagggcgg
     4681 ctgaagtcat agtttttgac ctgcttctcg tcctccccct ggatctggtc tacatccagg
     4741 tctccctcag acaactgttt cagaagatta tcaatttcac tttgtgaaag tatatcactc
     4801 acaggccctc acctcctgtc atcttatttt cccgcttcat cagccgaatt tcactccgct
     4861 gattgcgacc ttgtagatga agtcggattg gaatagatcc tgaacagact tcagaatctc
     4921 ctcttttaag ccttccatat cctctctgca ctcatcctct gtatgtgcgg aaacggtagc
     4981 cgtgatagca tccttgatca ggttctcata gcttgccaac gtgtcactgc tgccataggt
     5041 cttataatcc ttatgcttct tgttgataga caaagataca tcaaacatga tgtaatcctg
     5101 ttttgctcct gcttcgctct tcagaggaat tgtcatggag tctgcaatat tatatacctc
     5161 tgtatcggta agggcaacat tcgttgtttc ttcatccccc ggaacggtaa gttccagatt
     5221 catggcagtg gtaatattgt ctaccagttg tgcggtcttc ttattcgtac ccacaatgct
     5281 cacaagcatg acgctggtca acacaatgtt caccaccagc agtgccagaa tcaatactgt
     5341 catcagattt ttcttcattg gtcttatgct tcctttccct ctccacacta atatgttgtg
     5401 gatggattat agattctgat ctctacacgc cggttcttgc tgcgcccctc ttctgtggag
     5461 ttatcagcta ccggtacacg gtcacccatc cctgcgtgtc tcagatcaga tggatccaga
     5521 gaggtagtct ctgtcagata atagaacacg gaaagtgctc tggcgctgct caattcttca
     5581 ttatcggcaa acctcgcact gtgaataggg acattatctg tatgtccttc gatttctatg
     5641 gtggaattac ttccgtaacg ttccaggatc actcccacct tgtccagaac ttccttagcc
     5701 tgatccttca ggctgtcact tccggaatca aacaacagcg ctcccttcat agatagttgc
     5761 acatattgtg ccgtaaatga cacactgacc tgatctgcaa ggctgtcctc caccagagct
     5821 tcctgtactg tttcagccag ttcttcgttg ctctccagat ttttctcatt cagtgcctct
     5881 tccaaagctt ctgcgctgtc atatttctca taattatcag aatccgtctc actgtctgcg
     5941 tttcttccgg cattactgat atactcatcc agatcattca actggctgac gccgttgctg
     6001 atcaggatac cctcaccgat tgccgtggat ccggcattga aaatgctaaa agtgctgttc
     6061 gacatagctg ctgccatttc gctgtacttg gttgcatcta tcgaagacat ggaaaacaac
     6121 ataacaaaga agcacagcag caagttcatc aggtcactga atgtggtcat ccatgccggg
     6181 gatccagcag gtggatcttc tggcttacgt tttgccatta tgcttcaccc cctgcttcct
     6241 cttcaccatc tgcacttgtt ctgtccttcg gaggaaggaa tgacttcagt ttttcttcga
     6301 tgacacgggg attttcacca gcctgtatgg ataaaagccc ctcgatcata acttccttct
     6361 gctgcatttc cacagcatta tctgctttta atttattgga aacaggcgta cagatccagt
     6421 ttgccagcaa ggatccgtac aatgtagtaa tcaacgccac tgccattgca ggtcccagtg
     6481 tggacggatc gtccatgtga tacaacatat ctaccagacc gatcagggta ccgatcatac
     6541 cccaggcagg gcccatggcc gccagtgtat cccagaaacc gatcgtctct ttatgtcttc
     6601 cctctacact taccagttct gtctccatga tggcacgtac cagttcggga tcggtaccat
     6661 cgacgatcag aagaattccc tttttcagga aaggttcctc cagatccgtt gctgcttctt
     6721 ccagagacag cagaccttcc ttacgtgcca cattggaaag ttctatgatc ttgccgatca
     6781 tctcggaagt attcagtgcc ggtgccttga agatcagagt aaaggacttc agacccgcta
     6841 tgtaattctg taagctcatg gacatgaggg tacaggagaa agctccaccg aatgtaataa
     6901 ttgcagaagc cggatccaga tattctccga atcctccgac acccgcagag cttatgatac
     6961 caaataccaa catgaataac gctaataata ttcctgctaa agatgctata tccacttaaa
     7021 tcacctggca ttctgcaaat tattttgcaa aaatatcctt tctgtataat attacaagat
     7081 tttttatttc ttgtctactt tcttttacaa ttattttcct gcctgtggtg agagtcagca
     7141 ccgtatcagg ggtctcttct accagttcga tcaaatccgg attcaccaac accttgaccc
     7201 cattgatctt cgtaacttct atcatactgt tttctccttc cccattcatt gttcaatatc
     7261 ttctgtagtt tatgactttt accacattct ccaattttgt tatagtactt ttttcccttg
     7321 atcttttata catacaaaag ggacaggtag cattcctatc ccctttatac gtatattaca
     7381 tttttaacgt tttattaacg cttcagattg gtcagttctt ccagcaaagt atcggatacg
     7441 gtaatgatac ggctgttggc ctggaaacct ctctgagtgg tgatcatctc agtaaactcg
     7501 gaagacaggt ctacattact catctccagc tgaccggtgg acatgtatcc accgcctgcg
     7561 gtgatgtcta cgccgatacc atcgaactca ccggagttca gggttgcaga gtaaaggttg
     7621 tctccctctt tctccagacc ggaggcattg gcaaatgctg cggtagcaat ctggcctaac
     7681 agtttggtca taccgttgtc atagcttgcg taaatcatac catccttctg gatggataca
     7741 ccgatcatat ctcccagacg acgtccgcat ccggttcccg gaccatccag gtcaccactg
     7801 gtggcaccga tggtggaagt acccttgtta tcgtagttgg tgcattcgga caggtcgatg
     7861 gtaatatcgg agaagttgcc acctaactgt gaaaatgctg cattaatgcc caagtttgta
     7921 gtagaaccgc cgacactttc cagtttagcg gtgtctttat tgaataccac agtagttcct
     7981 tcaaaataac gaccttccat ggtaatagca ctgccgtctg ccggaagcag ctgatctccg
     8041 gtaccagatc tcatattgcc aagcagctgc tgcatggtaa gctgttgcgt tgtgtctgta
     8101 gggctggtaa tatacaaatt caaaaattca ttggtagatc cctcatatcc ataagctgct
     8161 gcaatggcat ccaaagcctc ctgctgttcc tttgctgcta tagcattgtt tgaagtatca
     8221 atcaggcttc catcagcatt aaaaattttc gtcaggtcag ctatatcttt ggttccatcc
     8281 tttgttttca gcatcgttcc atcccacttg taggcatcgg tattcaatgt caccttagtc
     8341 tccatcttct gctgggtacg gtttccgaaa tccagtgcgg aaatatcgat ctctgctccg
     8401 gtggaatcca gcagtttcgt cagttccata ctataaacat tggtgttcgg ttcgccagac
     8461 tgtttaaagg taaattttgc agtataactg taacctctcg catcgaagaa gttcaggttt
     8521 acggtcttac cgttggcact ggtcacatcc ttatcgttct catccagaat accggaaata
     8581 tttgcctgtg tggtagcctc aggaggatat gtcatattcg cagcactcat aatacgcagt
     8641 gcggttacgg tatcctgctt aatgttacct gtggtctcat ccacgcccca tcccatgacg
     8701 ttgtaaccgg tgctggtcat tgccaggttg ccggcaccat ctacatagaa agagccgtct
     8761 ctggtgaaga aattctcgga gccgttgctg actacgaaga agttgtctcc ggtgatcatg
     8821 atatcaaagg ggttaccggt agactgcgcg gaaccctgcg tggtaatggc agtattgata
     8881 gctcctgcct taacgcccag accgatctgc ttggcattgg taccgccgac accggtggta
     8941 gcgttggcac cactggcctt ctgtgtggtc tggctcatca gttcactgaa agtcatggaa
     9001 ctggatttgt aagctgttgt gttgacgttg gcaatattat taccgattac gtccattctg
     9061 gtctggtgtg tcttaagtcc tgctacgcca gaatacaatg atctcatcat agtgctttgt
     9121 tcctcctgta ttggaaccgt gctccctctc tgtcattcaa gggagcatga cctccatccc
     9181 tggtccggtc tacataataa cggctccgtt aatatttgta aatacgtttt cttcggtttc
     9241 tctgctgttc atggcagtca ccacggtctt attgggtaca ttcacaataa aggccaagtc
     9301 atccacgatc accagagaat ccctgattcc cttctgtccc gcttttcttg ctccgtcatt
     9361 caagcgttcc agctgtgcgt ctgtcagctc aatgcctctg ttttccagcc gtacggcggc
     9421 atgcttggag aatttcagat cctgcgttcc ggaaacattc tgttcctggc ttttgttctg
     9481 taggatctcc tggaaagata ccagattttc ctgtgctgct tttacggtct tgcggctgct
     9541 gtttaagtac tgatttgcca actggtcaat ggacagaaaa ctgttgttct gaatatccat
     9601 atccatctgc ccctctgcgt cagctgctta ctgcattata cgctctctgt gctgtcagtt
     9661 tcttctgact catccttgct ctcggtatcc ttcttttctt ctgcttcttc ggcagctttg
     9721 cggatctcct tgagacgttc aatgtactta ttcaagctgt tcacggtatc ttttgccagg
     9781 aaggatttct cgtaatcagt catatcgttg tattccttct ccagatcatc aatggtcttg
     9841 ccatcactgg aatcaatgcc gtttacatta ggaagcttgt tcagctttac ggtaaagttg
     9901 taagccttgt tgtacgcatt cagataatcc gtatccacta cggaatccag atcatcaatg
     9961 gagtacttct tttcgttgat ataaaggtaa gctttgttgt tctcgtagga cacataatcc
    10021 acttttccct gcaccagttt ggtatcgccg gaggatgtgg tggtcttgat atagacctcc
    10081 ttgcctacca gagaacttgc tctctgcaga tccatactgt tggacatatt ctgcatctgc
    10141 tctacctgtg agaactgtgc atactgtgct atgtattccg tattggacgt aggctccaga
    10201 ggatcctgat atttcatctg tgctaccaga agctgtaaaa atgcttcctt gtccattccg
    10261 ctgctggact ttgcagcttt actcacggaa gtctgggatg cggtctctac gatcttaccg
    10321 ttttccacgg gtgcaaccaa tgccatttcc tttttctcct ttcgttattt attatgcggt
    10381 aaaatccacc gtattgccgt tcgccgacat catatctgct gcgatccggt cttcttcggt
    10441 ctgaggctct tcttctgcaa atgctgcgtt cagattgatc ctgcgggtac gctttctacc
    10501 tgcacccgtt tcatttgccg tattactgtt attgcctctt ccctgctcca gattctgttc
    10561 gaactggtgg gtctgaacgg taacttcaat agcttctacc ttgacaccct gctctgcaaa
    10621 gttttccttc agctgtacca tctgactttc cagtgctgcc ttgactgttt cattctgtgc
    10681 aatgaaattg gccgtaacca caccgtttct ggatgccatc tgtatatgca gagttcccag
    10741 gctctgggga tgaagctgca tttccagttc agagctgtcc gccttgacgg atactttcat
    10801 ataatccatg atctgacgca taatgtcctg cgtatctgcg gttcttgctt cttctgtctg
    10861 tgccgtctgc tgtaactgtg ccaggaaatt gtcttccttc agattctgta ataacagatt
    10921 gccatgctgt ccaccgtcgt tgtgttcctg tctgctgccg gaatcttcgg tcttgttctc
    10981 ctgtgcgttc tgtacgggaa ctttttcccc gctgtcaggt acctgtgtca cggttgcttc
    11041 tgtggtatcc acagtagatt cttccgtagt aacggccaca ttctctgttt tatctgtttc
    11101 cacggtgatc accggttccg ccacagtttc ctgtgtgatc tgtgttaccg cttcctgcca
    11161 tttttcagga gatagctgta actcttcctg cgcctgccct gtcagttcct tctgcagatc
    11221 cataattcct tttacatcat tgtacagatt ctcatccgtc agtaatgcga acgaatcctg
    11281 tgctccgctg actgccatca gcaattcatt cagtttcccg ggatctgtca gatccacttc
    11341 tgtcattccc agatcttcca tggtggcctg cagttcttct tcactgacgg aaaatgtatc
    11401 cgtgatctgt tggatcaggt tctgtaccac cgttcccaca acttcctgaa ctttctccag
    11461 atcctttgca tccaggctgt catcttccat cgtcttgttt ccatcagaga catccgctac
    11521 attgtcgcgt tgtctgccgc cggctctcaa agattcacca cgtctgacat cgttccgtcc
    11581 cgcctgggtg cggtccccgt cggctgcatt agttgtctgg gcattgctct gggtcttatt
    11641 ccataccgcc tggaagctgg ttcccgcatt ctgcttcgct gctgtgacca tatccggtat
    11701 ctgcacattg ccgctgttac cgacaccttt aattgtcgtt cctgccataa ctcctccttt
    11761 cccaggcccc ttttaccacc ttatgacggt ctatgacctg taattatact acaaatcatg
    11821 tatcggatct tttctgcttt ttcttaagat tccggatcca taattttagt aatctttgcc
    11881 gctacttctg aattcatagc tcccaggatg gcacctctgt catccgcact catgaccttc
    11941 agaattctgg ctgccagatc cagattgttc gtcatctgtt caaaaattgc cgctgcctgc
    12001 ttcggcttca tggctgaata agcggccgca taatcctgca cttcctgact ctcctgcagc
    12061 tgagtcacta cctgtttgta aatatattcc gcagtggccg gatccatgga ctcataatat
    12121 ttcttatatt cttccgctcc gggtccctta tcggaataaa cgacctcgtc atagaattcc
    12181 ttctgaatcc gttggaattc cacctgctga ttttcgaatt ccttcagcct gagaacttcg
    12241 gctttcagct gatccagttc ttcgtccttg gcggaagatg cattctgtat ctgctccagc
    12301 tgtagttcca gactcttgat ctgatccacc gcatcctgta agctggtata gccgccgtaa
    12361 gcaccggaat tcgttgtctc agtcacagag ttgccgggaa gaatccggtt aagtaccgga
    12421 acatccttta agatcggtgt tactaccgag cttccaaagc cgccgatatc cagcttgatg
    12481 atcacacaga tcacagcaag ccacaatgcc acgatcagga tcgtggcccc gaaggtcaaa
    12541 aggctgttcc cctcattccc gtcctccagt tcatcttcct gtttggcaat ttcccttgcc
    12601 cggcgctttg cttcttttcg ctgggcctgc tgttctttct ttaaattttt tttctcttcc
    12661 accagctgtt ttctctcgtt atccgctgcg ttttcttccg gagttttttc tcttgccata
    12721 ctaaacctca tgcctttcca tcacctgccg gcttttcgcc ccgcatcccc gttctagcgg
    12781 ttcttctgtc cgtaggtgta actggtcagt tcatcaattg ctttactttc cgcatggttt
    12841 tcttcctgca gaaaagcctc aaatgccttc tcccgcagtg tctcatgcat ctttctttcc
    12901 ttcatggcct ctgccatccg ttctctggca gcttccacat tttcctttgc tttttccacc
    12961 cggattgcct gcgttgccac aatgctgtcc attttcaaaa gtgcttcctt gttctcttca
    13021 atggcccgca gatccagctt tccttttaac agccccgcag attgctcttc atactctctt
    13081 ctgcgttctt tcaagcgctc cagatgttct gtttccacat tcagagccgc ctgcgccgtt
    13141 ccgaattcct gtttcgcctg tgtttccatt ttttctttga tattcaggat gttttgcaga
    13201 gaatatctga atctcgccat ttatctcacc cctgatctgc gaacagctct tccagcatct
    13261 gtacggtctc ttcaaaggta aatttttcat ccgtaccctg gcataggaag ccgttcacag
    13321 catcgattct ggagattgca taatcaatat tcgggttgct gccctttttg tatgcaccga
    13381 tattgatcag atcctccgcc tcattatagg ttgccagtac atttttcagc ttgttggctg
    13441 cctgtttgtg ttccctggtg gcgatctggc tcatgcatcg ggaaatgctc tgcaaaatat
    13501 cgatagccgg gtaatggttc ttatgtccca gctttctgtc caacataata tgaccgtcca
    13561 gaatgcttcg ggcggtatct gtaatgggct cgttaaaatc atcgccgtct accagaactg
    13621 tatacaatcc ggtaatagag cctactgcgg ctcttccggc tctctctaac agcttcggca
    13681 tctccgagta cacactggga ggatatcctc tggtcaccgg cggttctccg ctggccagtc
    13741 caatctcccg ctgggccatg gaaaaacggg tcagggagtc catcatcagc aggacatcct
    13801 tgccctgatc tctgaaatat tccgcgatag cggtagctgt cttggccgcc ttgttacgct
    13861 ccagagccgg ccggtcggag gttgccacaa ccacaacaga tctgcgcata ccttcttcac
    13921 ccagatctct ctcaataaat tcccgtacct ctctgcctcg ctcgccgatc agtgctatga
    13981 cattgatatc cgctttggta ttgcgggcga acatacccat cagggtggat ttgcccacac
    14041 cagagccggc aaagatacca attctctgac cttttcctac cgtgatcatg ccgtctaccg
    14101 ccttgactcc caacggcagt acctgatcga taatagttct ggacatagga tcgggaggtg
    14161 ctgcctctac cggataggcg gctccattga tcatttcgcc gtcaataggc acgcccagtc
    14221 cgttcaccgt cttgcccagc agtttgtcgt ttaccgtgac cttcagggga tctccggtat
    14281 tttccaccac acatcccaat ccgataccat ctacggaatc atagggcata agcagtgtct
    14341 tcttgtcacg gaagcctacc acttctgcca tgatgggcct tgccccttcc gcatccggaa
    14401 ggatccggca cagatccccc agtttggaat ccggtccggc ggactctatg gtcagaccta
    14461 ccacattgac cactttcccc agcttacggt aaaaactctt ttctgccatt ttattatatt
    14521 tttcaaaatc aatgttctta atgaccattt atcagccttc gtaggataat aattttagtt
    14581 tctgacgcag ttccgacagt tgagtcccca gtccacaatc aaaaatgccg ctttcggtct
    14641 cgatcatgca ctcacctttg cccagggtca tgtcctcaac gatctccacc gtactgttcg
    14701 gtgcagttgc ccctgccatg atctgctttt tctgcatgct gacataggga taatcttctt
    14761 tggaaacatg tacgagaaag ctccggttgt cctctgtttt tctcatggta gcagaaatca
    14821 gatagcagag cacttctctg taagaatgca gatccacatc aaaaatatgc tcgtaaatac
    14881 ccgtaatggt atctacgaac tggggctcca gttcatccat tctcttctga tattccgcat
    14941 ccagtccggc agcttttttc tgatattccg ctgctctctg ggcctctgcg cgttcagcct
    15001 ccgctttcgc ttcctgctgt ccctgccttc tggcctgctc cagtgcgtct gctttctgtg
    15061 cttctgcctg tgctctggca tccgccagaa tgctctcagc ctttgctttg gcatcctcta
    15121 tgatcttctg agcctctgct tcggcttcct cttttaattt ctgcaggtct tctccggcct
    15181 tgatcacatt gccggaagtc tgcggatttt cttcgccatc cccggtgtcc gtcaccagtg
    15241 cctctacttt atctgccgcc agtccggaga taaatccggt tccttcgtcc tgctgcattt
    15301 tggcagccag ttcccggatc cttttcgcta ccagatcgtt ggtattgatc acccgggcat
    15361 cttcttccac aagctgtgta aatccccgtt ttaaaagatt actagacaac gatctcgtca
    15421 cctccacctc tggaaatgat gatctctccg gaatcctcca gcttacggat aatattgacg
    15481 atcttctgct gtgcttcttc tacatccttc atacgaacag gacccatgaa ttccatatct
    15541 tccttgatca taacaaccag acgtttggac aggttgttga agatagcatt ctgtacctgc
    15601 tcgttggcac ccttcagtgc cactgccaga tcgttgttgt ccacgtcacg cagcacacgc
    15661 tgaattgcct tgtcgtccag gagcaatatg tcctcgaaaa cgaacatctt ttttcggatc
    15721 tcgtctgcca gttcgggctc ttcgatctcc agagtctcca tgatatgttt ctcggtacca
    15781 cggtctacag tattcaggat atccaccact gcatccacac caccgataat ggtgtagtcc
    15841 tgattcacca gacttgccag cttggactcc agtacttttt ccacttcctt gatgacatcc
    15901 ggactggtac gatccatgac tgcaatacgt ctggctacct ctgcctggct gtcgggtggc
    15961 aatgcggaaa tgatcaacgc cgcctggctg ggtgacagat aagacagaat cagagcaatg
    16021 gtctgcggat gctcgtcctg aatgaagttg atcagctggc tggcatccgt cttacgcaca
    16081 aactcgaagg gctttacctg taaagatgca gtcagcttgc cgatgacatc catagccttg
    16141 tcggaaccca gtgccttttc cagcagttcc tttgcgtagt tgataccacc ctcggcaata
    16201 tactgctgtg ccagacacac ttcgtaaaat tcgttgatca cttcttcttt gacctgagga
    16261 gtcacacttc tggtattggc gatttccagg gtcagttctt cgatttcttc ttcttttaag
    16321 tgtttaaaaa ttccggcgga tctctccggt cccagagaga tcaggaggat tgcagctttc
    16381 tgcagtcccg atattccacc ttctgctttt gtccttgcca tagttactcc gtttcttagt
    16441 tccagtcctc gttcagccag ttacgcaaca atgccgctgc ggattccgga ttctcatcta
    16501 cgaatttttc aatcatctta cgtgtctcag acttagattc cacatcaata tcttccagct
    16561 ctgcttcagg ggtagactgc agcatattct ccacagatac ctcttcctcc tgctgtaccg
    16621 cctgtctggt acgcatgctg tgcaatatca caaatgccaa cagtcccagg atcaacagca
    16681 gcataagcac gctcaacaca ttctgtgccg taaccgggct gctttcctta tcgtaaaaaa
    16741 taggactttc gtaagcaata atcgtaatat tactctcatt gataccggtg gcattggcta
    16801 ccaggctgta catatcctga tcaactgtca tcttggtatc tgcgctgttc tgggatttgt
    16861 attcatccca ggtagtgcca tccagaagcc cctgtcgttt tacatcctca taatgaattt
    16921 ctttataagt aattgctgca atagatatgg aggaattagt gtagttgatt cctcctgccg
    16981 gtgtcacagt atccttaata gactcattcg gcagataatc ccggctggtt tcggaagtag
    17041 aggaagaact gttgctgctg tccggagata cataggtagt atttccgctc tccccgttgg
    17101 aagttgtccc cggtacaccg ccgccgtcat tggtattctc gctctcataa gtctcctcat
    17161 gggaaagcat accttcatct ctgccggagt ttgcataata ctcttttacg gtctccttat
    17221 aatcagagaa atccatgctc aggtggctgg taacggccgc atcattatac tgttttgttc
    17281 ccagcagtac cttcttgacc tgattggcga tcatggattc cgcctgattc tgcagctcct
    17341 gcatagaatt agcgattcct gcggtagaat aatcatctcc acctgcaaac agcaggttgg
    17401 aatctgtatc cataatagtg atattcgccg tagtatcgtt gcgcagccag gtagccacac
    17461 attttgccag tgccgcagca ttggcggagg taaaggtgcc gtccagttcc agttggatga
    17521 aggcagacgc ctcttccttc tgtgctgcca gagttccgtt atctttcggg aaactcagat
    17581 gtaccttggc ggattttact gcgctctgtg cttctatcgt ctgtgttatg tatttttcca
    17641 gataggaatt gtaaagtctc tctttgtcag tggcggtcgt agacatacta ccgcccagat
    17701 aatcattcaa atctaacgca tccggtacat atcctgcgga acccagtgcc aagtttgccg
    17761 cactctgttg gctgctctct acatcgatct tcagtccgtc attggattct ttatgtgcaa
    17821 ttcccgcact gtccagagta tctatgatct ccgaagcctg tgatgttgtc tcacaggtga
    17881 tcagccgtac atactggggc ttactcataa cgtacatgat gattgcaaat gcaaatatca
    17941 caaccgccgc cgctccaacg atgatgctct tctgcctggt ggtgaatttg ttccaccatt
    18001 ccagtatttt tgcaggtatt tcttttaatt tgtctgccat tgcagctctc tcctcttacg
    18061 tcaagaataa aaatacttct ttagatctgg atctgcatca gttccttgta tgcttccatc
    18121 accttatcgc ggatggctac ggtatactgc aatgcagtcg aagccttatc catagcaata
    18181 gtaaggtcgt gggtgttgtc ggtctcaccc aatgcaaatc gtatcttctc attctcggca
    18241 tccgagagat aactgttggt gatcttcaca ttgtcgattg ctgtattcag aaaagtttcg
    18301 aacatagtgc cttctgcctt tttctccttc tccgccagca ttccggtcag agaagtggga
    18361 gatactgtgg cagtaccgtt tacactgttc aaagctgtaa ttgctgaaat atccatttta
    18421 ataaaccatg tccttcctca catatattcc ggcgccgcta tccggagccc ggtaataaag
    18481 acccgtcagc ttactgaccg atctccagtc cttttaatgc catggacttg ctggcgccaa
    18541 atgccgtgga attggcttca taggatctgc tggcatcgat cagatccgtc atttcctgaa
    18601 caatatttac attcggatat gtcacatagc cgttctcatc cgcatcggga tgggaaggat
    18661 cgtataccat attcatctcc gtagtagtat cttcaaagat ctggcttacc ttcacgccgc
    18721 tgccggtata actgctactc acattgtgca gcacctgact gaaagtctgc tgctgcccct
    18781 tctgctggaa agtaacgatc ttgcggcgat agggggtgcc atcctcggtt cttgtagtat
    18841 tggcattggc aacgttttcg gagatcacgt ccatacgaag tctctgggca gtaagtcccg
    18901 atgccgtaac atcaaatgct gaaaacatac tcatacgcta attcctcact tcatcactgt
    18961 ctttaaatta gtaaattcct gattaatgct gctgatcagt ccctggtatt tcagctggtt
    19021 ctccgccagc attacatttt cattttcaat atccacgtta ttgccatcca ggcgatagga
    19081 ataattggca tagtccaagt aagccctgcc ccgcagtcgg ctcagatcca cattggccac
    19141 cttatcatcc atggactcat atctgttatt gccaagagcc tgctgcaata ccgattcgaa
    19201 agccacatcc tgcctcttgt agcccggagt atccacattg gcgatattat tggaaatggc
    19261 ttcgttccgc tgccaggctg cgtccgctgc ccggtccaga acattgatat aatcaaatgc
    19321 gtttgtctgt atcatattac ctcacattcc gcggtcttct gacaagagca cccaaaaacc
    19381 gctttctacc atatacccat tttattttat tataaagtat ttcgacaaaa acacaatagt
    19441 aaaataaact cacacagaat aaatataaag aaaaaagact atttttcgca atagtctctt
    19501 cttcctaaat ttttcttatt cttgcaaatt cttacatttt tcatttaatt tctgccgttc
    19561 tgacgcttca gttcctcgat ttcatcgaat acggcatcat tcaaaacttt aatataggtt
    19621 cctttcatac cggaggaacg ggattcaatc acaccggcac tttcgaactt ccgcagggca
    19681 ttgacgatca cagatctggt gataccgact ctgtcggcga tcttgctggc caccaggatt
    19741 ccctccatgc cgttcagttc gtcgaaaata tgggtgatgg cttccatctc tgagaaagaa
    19801 agagtgctga ctgcagattt tacgaca
//
LOCUS       DAWDHD010000031        19690 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_193_length_19690_cov_12.159155, whole genome shotgun sequence.
ACCESSION   DAWDHD010000031 DAWDHD010000000
VERSION     DAWDHD010000031.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 19690)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 19690)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..19690
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_193_length_19690_cov_12.159155"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         5711 a         4063 c         5372 g         4544 t
ORIGIN      
        1 ggtagatacc gaggaaggtc ttggatgggt ctctacagaa tttgtttccc tgcatacgga
       61 attcgtagaa gcagaatcca aagaggaaga agcaaagcgt ctggcaaagg agaaagaaga
      121 gagagaaaga gcacgtgccg cagcggcagc caaggcagcc acaagcagca agagttctgg
      181 cgcttccggc agcgctcccg cagttactta tgcggccagc ggagacagcg agatgggtac
      241 cgcagttgcc gactatgcat tacagttcgt tggtaaccct tatgtatacg gcggatctag
      301 cctgaccaac ggcactgact gttccggatt tgtaatgagt gtatatgcta atttcggtgt
      361 aagtctgcct cattcctctt ctgcagaccg tagccagggt tccgcggttg acggacttgc
      421 caatgcacag gcaggtgacc tgatctgtta ctccggccat gtggcactgt atatcggtaa
      481 cggccagatc gttcatgcat ctaccgccaa aaccggtatc atcgtatcta atgcggatta
      541 caagaaggta ctggcagttc gacggatctt ctgattatat aacagaaagt acaaggcact
      601 gtgtggaaac acacagtgct ttttgtgtat acgatgagcg agactactcg attgcaccgt
      661 gtcatgtggt cggtttattt tccatgtgga caggatgctg tggggacagt ggaacttaag
      721 tgccgataag gcaaaaaaat ataagttgta taacgaattg tgcaatttgt caaaatattg
      781 ttacgcgatg aaatggggat tgacaaaacg gaactataca aagtatacaa aaaaagtgac
      841 acgaacgtaa aaacgacaga aaagaaattt aaatacaaaa taagttatcc acatccaaaa
      901 tgccagaaaa taggataaaa gttacttata caccaagtta tacacattat ccacagaaaa
      961 accggcactg caagggggat aaatttacga aaaaacagga acaaatgttt tgtgaacatg
     1021 taataaaaat aataaaatga caggatttgt cgattttagg gttgacatac agaatgtcaa
     1081 aaacaagtaa aaaacttctt aaattgttgc aaaaagctgt gctattgtgc tataatgact
     1141 atacaataaa tacaagggat gctacataaa accttgtaaa taaatacttg gaagggatga
     1201 tcagcatgaa gtttattatt gttggcagaa acatcgaagt tacaccagga ttaagagcag
     1261 cagtggagga aaagatcggc aagctggaca agtatttcaa tcccgacaca gaagtacatg
     1321 taacattaag cgttgaaaaa gaacgtcaga aaattgaggt gaccattccg gtaaagggaa
     1381 gcattatccg ttcggagcag gtaagcaacg atatgtatgt atccattgat ctggtggaag
     1441 agatcattga gagacagttg aaaaaatata agaacaagat cgttgacaaa cagcaggcgg
     1501 cagcttcttt cagcaaagcc tatgtagaga atgattacac agacgatgac gagatcaaga
     1561 tcgttcgtac caagaagttc gatatcaagc ccatgtatcc cgaggatgcc tgcattcaga
     1621 tggaactgtt gggacacaac ttcttcgtat tctgcaacgc agagacagat caggtaaatg
     1681 tagtgtacaa gagaaagggc gatacctacg gactgatcga gccggaagta taaaaccgga
     1741 cagaaaatta aataaagaca aatttacagc ctgccggagt ttccggcagg ctgttttgtt
     1801 gtatgggaaa tgatatgccc cggagatttt cgaattgtct gaataccgga aaaatcagaa
     1861 acataacctg taaggaggaa ctgagagcag gacagggaat ggacagagaa aagagaatgc
     1921 gggcatggat ccgggcagag gtgatgcttc tatttataat gatgggagca tttctgctcc
     1981 gggagacagg gcggacagaa agcatggaac aggcggtggt caccgcgacc tcggctgcgg
     2041 gaaaggatta tatcaaatgg gtggacttta ccgtatccta tgaagcgctc tgccaggcat
     2101 atgactggga tgtggatacg tttgacacgg aacatcatgt ggagtggatc ccgctgcttg
     2161 catataccgc agccagaacc ggaggggaat ttgacaaaaa ggcgctgaag atcctgaacg
     2221 agacggcgga gaagctggct gagggagagg cagagataga aactctcaca aaagatatga
     2281 aatactatcc gtattatctg gaagcttaca gcgcggcact gggaggactg gtgggagaat
     2341 acgaggcaga ggtgatcgga gaagacggac agagcacctg gcagaaaaaa tacggattaa
     2401 aaggttattg ccctattgcg agaggatttg attatactca ttatgatgat ttcggggcgg
     2461 gaagaagcta cggatataaa cgaaggcatc tgggccatga catgatgggg ctcgtcggtg
     2521 tacctatcat tgctgtcgag tccggcactg tggaagccct gggctggaac cagtacggcg
     2581 gttggcgcat tggtatccgc agctttgata ataagcgcta ctattactat gcacatctgc
     2641 ggcagaacta cccctatgcg gaaggactgg aagaaggcag cgtggtgacg gcgggggatg
     2701 tgatcggtta tatgggacat accggttaca gtaccacaga aaatgtaaat aatatagagg
     2761 taacccatct gcactgggga ttggaactgg tctttgatga agaacagaaa gagagtgaca
     2821 acgagatctg gatcgatgtc tatccgctga cctgttttct ggcaaaacac acgcaggaag
     2881 tgcagaaagt cgagggaaca agagagtgga tccgtactac aaaaatccgg gaattctgat
     2941 ttctaaaaat ttcatttgtt attttgttat ttttttgaca aaagcattgc aaaacagatt
     3001 tcgctatgtt aaaatgggga cagtgaaatg ataaaaaaat aacaatctac atttcagaaa
     3061 gcaaaccaaa cgaaaacaaa aagtggagga caaacaaatg gcaaagaaag ttgtattagc
     3121 aggcgcttgc cgtaccgcaa tcggtaccat gggcggaacc ttaagcacaa ctcctgcacc
     3181 ggagctgggc gcaatcgtta tcaaggaggc tctgaagaga gcaggcgttg ctcccgaagc
     3241 agttgaccag gtatatatgg gatgtgtaat ccaggctggc cagggacaga acgtagcacg
     3301 tcaggcagct atcaaagccg gacttcccat cgaagttccc gcagttacca tgaacgtggt
     3361 atgtggttcc ggcctgaact gtgtgaatca ggcagcacag atgatcatgg ccggtgatgc
     3421 agatatcgta gtagcaggtg gtatggagaa catgtccatg gctccttacg caattcctca
     3481 gggacgttac ggatatcgta tgggtaacgc aaccatggtt gataccatga tcaaagatgc
     3541 actttgggat gctttcaatg attatcatat gatcaagacc gcagacaaca tctgcgaaga
     3601 gtggggactt acccgtgaag aactggatga gttcgcatta aagagccagt taaaggctga
     3661 agaagctcag aagaacggtg ccttcaaggc agagatcgtt cccgtagaag ttaagaagaa
     3721 aaaagagacc atcgtattcg ataccgatga aggccctcgt catggttcta ccatcgaagg
     3781 tctggctaag cttcgtgcca tcaatcccgg tggattcgtt accgcaggta acgcttccgg
     3841 tatcaacgac ggtgcagcag ctatcgttgt tatgagcgaa gagaaggcta aggagctggg
     3901 cgttaagcct atggctactt tcgtagcagg cgctctggct ggtgttcgtc ccgaggttat
     3961 gggtatcggc cctgtagctt ctaccaagaa ggtaatggct aagaccggta tgaagatcga
     4021 agacttcgat atcatcgagg ctaacgaagc attcgcagca cagtccgtag cagttggtaa
     4081 ggaactgggc atcgatgtag ataagcagct gaaccccaac ggcggtgcta tcgctctggg
     4141 ccatcccgta ggagcttccg gatgccgtat tctggtaact ctgctgcatg agatgcaggc
     4201 aagaggtgct aagaccggtc tggctactct gtgcatcggc ggtggcatgg gctgctccac
     4261 catcgttaag atcgaggatt aattttataa atattcgctg gggcggtaga atctctatcg
     4321 ccttagtgtg gttttgaaaa cacgaataga aatgtgaaat gaggtaaaaa catggattac
     4381 attttgtatg agcagaatgg caatgtggca accatcacca tcaaccgtga aaaggcactg
     4441 aacgctctga attctcaggt actggatgaa ctgaacgcta cattggatgc agtagatctg
     4501 gcaacggttc gctgcctggt gatcaccggt gcaggcagta agtccttcgt ggcaggtgca
     4561 gatatcggag agatgagcag cctgaccaag gcagagggcg aggcattcgg taagaagggt
     4621 aacgatgtat tccgcaaggt cgagaccttc cccattcccg tgatcgcagc tgtcaacggc
     4681 tttgctctgg gcggcggatg tgagatttcc atgagctgtg atatcagaat ctgttccgac
     4741 aatgcagtct tcggacagcc cgaggtagga ctgggaatca ctcccggctt cggcggaacc
     4801 cagaggctgg ctcgtctggt aggtgcaggc atggcaaaac agatgatcta caccgcacgc
     4861 aatatcaagg cagcagacgc ttacagaatc ggtctggtaa acgaagttta cagcgcagaa
     4921 gtagatgcag acggcaatgt ggtaaagagt gcacaggaag taatgctggc agcagcacag
     4981 aagatggcag ccaccattgc aaagaatgct cccatcgcag tccgcaactg caagaaggct
     5041 attaacgaag gactggatgc agatatggat caggcagtag tgatcgaaga gaagctgttc
     5101 ggtgactgct tcgagacaga agatcagaag tatggtatgg ctttcttcct tgacaagaac
     5161 aaggagaagg taaaagagcc ttttaagaat tgctaatcat tacgaatata aaagtataaa
     5221 atcaggagga tttaccatga aagtaggtat tatcggtgca ggtactatgg gacagggcat
     5281 tgcaaaggca tttgcccagg tagacggata tgaagtagct ctttgcgata tcaagcagga
     5341 gtgggctgac ggcggcaagg ccaagatcgt aaaaggctat gcaagactgg tagaaaaagg
     5401 aaagatgact caggaggctg ttgacgctat cgtagctaag atcactccgg gcctgaagga
     5461 agatttgtgt gcagactgcg atctgatcgt agaagctgca tttgagaaca tggaagttaa
     5521 gaagaccacc ttcggtgagc tggacaagat ctgcaagccc gagtgtgtat ttgcttccaa
     5581 cacttccagc ctttccatta ccgagatcgg caacggactg acccgtcctc tgatcggtat
     5641 gcacttcttc aatcccgcag accgtatgaa gctggttgag gtaattgcag gtgtgaatac
     5701 tcccgcagag accgtagaca ccatcaagaa gatatctgaa gagatcggca agacccctgt
     5761 tcaggtaaac gaggcagcag gctttgttgt taaccgtatc ctgatcccta tgatcaatga
     5821 agcagctttc atcaagatgg aaggtgtatc cgatatcgca ggtatcgacg cagctatgaa
     5881 gctgggcgct aatcatccca tgggacccct ggaactgggc gatttcgtag gactggatat
     5941 ctgccttgcc atcatggatg ttctgtacaa cgagaccggc gacagcaagt atcgtgcatg
     6001 ccctctgatc cgtaagatgg ttcgcggcgg taaccttggt gtgaagaccg gcaagggctt
     6061 ctatgtatac aatgcagacc gcaccaagac tcctgtggat gcacagtagg caaaaggtaa
     6121 aataactgtt cggagccgga tacggctctg gacagtctaa gttcataatt acaaccaagt
     6181 ataaataaca atggaggatt aacatcatgg attttacatt atccaaagag catgagatgg
     6241 cgagacagct gttcaaagag ttcgccgaga acgaagtaaa acctcttgcg caggaagttg
     6301 acgagaccga aaccttcccc agagagaccg ttgagaagat ggctaagtac ggcttcatgg
     6361 gaattcccgt tcccaaggag tacggcggac agggctgtga taccctgacc tacgctatgt
     6421 gtgttgagga gctttccaag gtttgtgcaa ccaccggcgt tatcgtatcc gcacatacct
     6481 ctctgtgtat cgatcctatc atgaccttcg gtactgagga tcagaagaag aaatatgttc
     6541 ccgatctggc aagcggccgt aagatcggtg ctttcggtct gaccgagccc ggtgcaggta
     6601 ccgatgctca gggacagcag actaaggctg tattagatgg tgacgagtgg gtcatcaacg
     6661 gatccaagtg cttcatcacc aacggtaaag aagctgatgt atatattgtt atcgcagtta
     6721 ccggtatcgt agagaagaga ggcagaaaga tgaaggagat ttcttccttt atcgttgaga
     6781 aaggtactcc cggatttacc ttcggtacca aagagaagaa gatgggtatc aagggttctt
     6841 ccacttacga gctgatcttc accgactgcc gtatccccaa ggagaatatg cttggtaagc
     6901 agggacaggg attcaagatc gcaatgcaca ctctggacgg cggacgtatc ggtatcgctt
     6961 cccaggctct gggtattgca gaaggcgctc tggagagaac cgttgcttac accaaggaaa
     7021 gaaagcagtt cggaaagtcc atttccggat tccagaatac acagttccag ctggctgata
     7081 tggctaccaa ggtggaagct gccaagatgc tggtatacaa ggctgcacgc aagaaggacg
     7141 agtacaagac caatcctaag atctcttact ctgtagaagc tgctatggct aagctgtatg
     7201 ctgctgaggt tgctatggaa gttactacca aggctgttca gctgcacggt ggttacggtt
     7261 acatcagaga gtacgaagtt gaacgtatga tgcgtgatgc aaagatcacc gagatctacg
     7321 agggtacttc cgaagttcag agaatggtta tctccgcaga tctgctgaaa taagcaagca
     7381 ggcatttgtc ggaaatttta ttagaaaagt aaggagaaaa tacaatgaag attgttgttt
     7441 gtattaagca ggtgcccgat accaagggcg gtgttaaatt caatcctgac ggaactcttg
     7501 acagaggcgc aatgcttgct atcatgaatc ccgatgataa ggctggtctg gaagctgcac
     7561 tgaagctgaa ggacgagttc ggcgcagaag ttaccgtact gaccatgggt cttcccaagg
     7621 cagcagatgc tcttcgtgag gcgctggcta tgggcgctga caaagcaatt ctggttaccg
     7681 acagagtact gggcggtgct gatacctggg ctacttccac taccattgca ggtgcactga
     7741 gaaaccttga ctatgatctg atcattaccg gtcgtcaggc aatcgatggt gataccgcac
     7801 aggttggtcc tcagatcgct gagcatctgg gacttcccgt aatctcttat gcacaggaca
     7861 tcaaggttga gggagacaag gtgatcgttc agagacagta tgatgacaga tatcatgtag
     7921 tagaggctca gatgccctgc ctgatcactg cactgtccga gctgaataag cctcgttata
     7981 tgactcccgg cggaatcttc gatgcatgca aggcagagat cactacctgg ggcagagctg
     8041 atctgaaaga tgttgatgac agcaaccttg gcctgaaggg atctcctacc aagattgcaa
     8101 aggcatccga taaggttcgt aagggcgcag gtgagaagat cgttcccgat tctcctgaag
     8161 acggagcagc ttacattgtg gctaaattca aagaaaagca catcatctaa tataggaggc
     8221 aaaccatggt aaccaataat attgaagaat acaagggtgt atatgtgttc gcccagcagg
     8281 tagacaatga gatcagcggt atcgcattgg agctgctcgg caaaggtaaa gagctggctg
     8341 caaagctgga gaccgaggta accgcggttc tgatcggtta taatgtaaag aatctggcag
     8401 ataagctggc tgagtatggc gcagataagg taatccttgt ggacgatccc gaactggaga
     8461 cttacagaac cgagccttat gcacatgcac tggcatctgt tatcgagaaa tataagcctg
     8521 agatcgttct ggtaggtgca actgctatcg gacgtgacct cggccctacc gtttccgcaa
     8581 gagttaagac cggtctgact gctgactgta ccgtacttga gattggtgat ttccctctca
     8641 atgcgattcc cggacaggag cagaagcaca accagctgct gatgacccgt cctgcattcg
     8701 gtggtaacac catcgctacc atcgcctgcc ccgacaacag acctcagatg gcaaccgttc
     8761 gtcccggcgt tatgcagaag atcgctccta tcgcaggtgc aaaggctgta atcgaagagt
     8821 acaatcccgg atttactccg aacaacaaat atgtaaccat caaggaagtc gttaaggctg
     8881 tatccgatac cgttgatatc atggatgcta agatcctggt atccggcgga cgtggagttg
     8941 gcagcgcaga gaacttcaag attctggacg atctggcaga ggttcttggc ggtaccgtat
     9001 cctgctcccg tgcagttgta gataatggct ggaagcccaa ggatctgcag gttggacaga
     9061 ccggtaagac cgttcgtcct cagatttact ttgcaatcgg tatctccggt gctattcagc
     9121 atgtagcagg tatggaagag tctgatctca ttgttgccat caacaaggat gagaccgcgc
     9181 ctatcttcga tgtggctgac tacggtatcg taggcgacct gaacaaggta gttcccgctc
     9241 tgaccgagca gctgaaggct gcaaaggctg agaaggcagc aaactaattc gtaacaacgg
     9301 attaaaaaca tataagaact ccgcctgatt ccccggtccg tccgggagtc gggcggagtt
     9361 cttttctgca aaaaactgat gcaacttgtg caagtttctt gccttgtatg cttgcgctga
     9421 gggaagcaaa atagtaaaat aaaatgatac caagggtaac aaggtgaaaa ataaattttt
     9481 cacctgcaaa tttgtgcctt cggctcaaag tttgcaggtt gttgaaaggc atgagtagtt
     9541 ttatggcaaa acaaaaagaa gtaaagacca acgcaatgcg gatcctggaa tctttgaaga
     9601 ttccttatac ccatcacacc tacgagtgtg aggaattcgt ggacggtctt cagatagctg
     9661 acaaattatc cctgtcctat gaacaggtct ataagacgtt ggtcaccgtg agcaacagca
     9721 agaattattt tgtattcgtt atccctattg cggcggagct ggatctgaaa aaggcagcga
     9781 aatcggtagg ggaaaagagc gtggaaatga tccacgtaaa ggacattaac gccattaccg
     9841 gttatatccg cggcggctgt actgccattg gcatgaaaaa gcagtacgtg accagaatcg
     9901 atgaaagcgc aaagacacag gagcagatct atgtcagcgg cggcaagatc ggatctcaga
     9961 tccgtctgac accggaggat ctggccaagg cagcgaaagc agaatttgcc gatattatcc
    10021 ggcatgaaaa ttaaccggga agataccgta aggatctgac aattacgaaa acatatataa
    10081 aggagtatct atggaaaaag aaaaaacaat tctcgattac gacaccgtgg cactggatga
    10141 agcacaggat gcggtgatca tcatagacca gaccttatta cccggcagga tcgaactgat
    10201 cgctttacat acggcacagg aaatttggga tgccatttat ctgttgaaag tcagaggggc
    10261 tccggccatc ggcgtagcgg cggcactggg tatttatctg ctggcaaacc gcatcgagac
    10321 cgaagatttt gaaaagtttt atgagaaatt taccgaatat aaggaatatc tggattcttc
    10381 cagacccacc gcagttaatc tgtcctgggc gctgaagcgc atggatgctg tggctgtaaa
    10441 ggctggcaga gaagagcaca agactgtcgc aaaagtgaag gaaatcctgc atgacgaagc
    10501 actacagatt cgggcggaag atgtggaagt atgtcgaaaa atcggagaat ttggactgga
    10561 actggtaaaa ccgggagatg gtattctgac ccactgtaat gcaggacaac ttgcaaccgt
    10621 aaaatacggt accgcaaccg caccgattta tctgggacag gagagaggtt acaacttcca
    10681 tgtatttgcg gatgagacca gacccctgtt gcagggagcc agactgactg cttttgaact
    10741 gcattccgcg ggcgtggatg tcacactgat ctgcgacaat atgtctgctt ctgtaatgtc
    10801 caagggctgg gtacaggcag tgttcgtggg ttgtgacaga gtggcggcta acggagatac
    10861 ggccaacaag atcggcacca gcgtagtagc agcggtagca aagcagtacg gtgtaccggt
    10921 atatatctgt gcacctacat ctaccattga catggctact gttaccggcg cagatatctg
    10981 catcgagcag cgcaaggcgg aagaggtcac cgagatgtgg tataaggagc gcatggcccc
    11041 ggaaggtgta aaggtataca atcccgcttt tgatgtaacg gatcatgaat tgatcgctgg
    11101 aatcgtaaca gagtacggtg tggcaagagc gccttacacc gagagcctga aggagatcat
    11161 ggaacgcaag gcacagagag gctgatggta aagatatgaa gcgaaatgta agccttgcgg
    11221 agatatccga cggccgatta tacaaagcaa atgatatggt aaaagcggat tgcggtggtt
    11281 gcaatggctg ttcccggtgt tgtcacggca tgggcaattc cattatcctg gatccctatg
    11341 atgtgtaccg gatgactaca ggaatgggga aaagcatgca ggagctatta gcggcatcgg
    11401 tggagctcaa tgtggtggac ggtgtgatct taccgaatct gaaaatgcag ggagcagagg
    11461 aagcctgtgc gtatctggac ggagagggtc gatgcagtat tcacccgtac cgtcccggaa
    11521 tctgcagact gtttcccctg ggacgatatt atgagaatgg gggattttct tattttctcc
    11581 agaccgggga atgtgcgagg gagaatcgca gcaaggtgaa ggtcagtaaa tggatcgata
    11641 ctccggatct gacccgcaat cagcaattta ccttacaatg gcatgacctg ttacatgcca
    11701 tggaagaaag actgacagga gaagacgaag ggaaacagag ggaagaaaat ctcctgctgc
    11761 tgcgtctgtt ttatttattg ccgtatgagg ggacgataga tttttacaca cagtttacag
    11821 agaggatgga acattggaat ggacagaacg gacagattta acagtttaaa caagctctcc
    11881 attgagaagc gcctattgaa agtatttcgt aagaccacga tactgctgag catgacgggt
    11941 gtcatcgcct gtgtggccat gggaatcgtg agttatcagt attcctacgc cttgaagcat
    12001 tacggtttcg cgcagggtga catcgggaag gccatgatcg ttatcgcgga gatgcggagt
    12061 gagacccgtg ccgccatcgg ctatgatgac gatacactga tcgctacagc aaaaaatgcc
    12121 catgacctga cggcggaaca gttggatacc tatatcagtg ccctggaaga tgacatgatc
    12181 acggatgagg gaagagctac ctaccagcag atcaaggacg gcgtagcttc ttatcagcag
    12241 atcgaggcac agatactgga actgggaaca gcttccgatc ctgctaagcg gatgcaggca
    12301 cagcagatgg caggcgaaca gatggatccc gtgttccagc aggtctatgc agatatgacg
    12361 agcctgttgg acagcagtgt aacgaacgga aatgccatgg aagccaaact gaatgttttc
    12421 cgtgtggtta tttttattgt aatccttctg atcatcggag taggctttgc gggatctgaa
    12481 aaagcgggaa aaagaatagc caaaggaatc gccggaccgc tgaaagcgct ggcagacaga
    12541 ctggatgggt ttgctgcagg tgatctgtct tcagagttcc cggaagtaaa gacagaggac
    12601 gaagtggcag agatgaatcg tgccgccatt gcaatggctg aaaatctacg aatgatcatt
    12661 gaggatatca agcaggctct gaatgccatg gcaaatggtg attggacggt aaaatttctt
    12721 taccccgatc tctatgcagg ggatctggat gaattgaagg aagccctgag agatatcaaa
    12781 tataagatga acgaagcatt gtacaatgta aattccgtat ccggccaggt atccatgggt
    12841 gccggaagcc tggcagatgc cgcacagtct ctggcagagg gcgctactga gcaggccgga
    12901 gccgtacagc agctacaggc aaccattact aatattacct ctatggtaga caatactgca
    12961 gagcatacca agaattccag caggcaggca caggaatact ctgctaaggc agatgaaggc
    13021 cgtaccgaga tgaatcatct gatggagatc atggaacaga tcaacgagac ttccgagaag
    13081 atcagcaaca tcatcggtga gatcgaggat attgccagcc agaccaacct gctgtcctta
    13141 aatgcgtcca ttgaggcagc cagagcgggg gaggccggaa gaggttttgc tgtggtggca
    13201 gaccagattg gtaagctggc agagcagagt gcgcagtccg ctgtcaatac cagagagctg
    13261 atcggcggta cactggagga gatccatgcc ggcaatgaag ctgtggatca tgcgtcccag
    13321 acgttgagcg ttatcgtgga aggcgtccgc aggatcgcac aggattctga ggaactgaac
    13381 cagattgcag ctgctcagtc agatgccatg aatcaggcgg agagcggtgt gaaccagatc
    13441 tccgagatcg tacagtccaa ctctgcggca gcagaagaat tatccgctac cagtgaggaa
    13501 ctgctggctc agtccgagaa tatgaccaat ctggtaaagc agttcaagct gatcgatgag
    13561 gagaagattc aggcgagaaa aaaggaggct gacaatgccg gtatttgatt tcagcagtat
    13621 cccggaaaat gagaagaagc aggatcccgt atgcacctat accatgttcc gttccaatga
    13681 ggctgcggca agtcccgaaa agccgatcct gatcctggac aacagcagcg agcagtggaa
    13741 gcaccatgct atcggtgtct tcggcaatca gaacaaacgc acgaatttcg aattcaggga
    13801 agagggcagc agtgcctatg ccgatatttt acagatcgat gcaagattcg taagtctcgt
    13861 cagatggtta ggagaaaatc acatcaatgt caggctttcc ggggaaaacc gggaagaagg
    13921 ctatgcggta tacaagatcc gagagactgc atttggcggc ggaaccaagc tttcggcaga
    13981 agatggattt ttacagttca tgatggaaag actgttcgcc agcagtgctc ccgcagaaga
    14041 ggaacagggg gaggacgaag aggaagacgg cgatgatatg aagcttacca gccttcagag
    14101 tatctccgat ttcatgacat gcgccggaag gaccatgccg gaaatcatcc gcctgtgggc
    14161 cagaagaaat ctggcggtgg ccagatccca tgaggtgtct ccggaagaga gaagacatgc
    14221 ccagagagcg ctgtccatca tgatgaacat tcagtggaag ggcaattact tcgaagccat
    14281 tgatccggtg gaagccagac ggatcctgga cgaggaattg tacggcatgg agagagtcaa
    14341 gcagcggatc atggagacca ttatccagat taaccgtacc cacaccttac cggcttacgg
    14401 actgcttctg gtgggtccgg ccggaaccgg taaatcccag atcgcttatg cggtggcgag
    14461 gatcctgaaa ttgccctgga caacattaga catgagttcc attaacgatc cggagcagct
    14521 gaccggaagc tccagaatct atgccaatgc caagccgggc attatcatgg aggctttttc
    14581 catggcggga gagtctaatc tggtattcat catcaatgag ttggacaagg cggcaaacgg
    14641 caagggtaac ggcaatccgg cggatgtgct tctgacactg cttgacaatc tgggattcac
    14701 ggacaactat atggaatgta ccgttcccac ctttggtgtc taccccatcg ccactgccaa
    14761 tgacaaggag cagatcagtg ctccgttaat gtcccgtttt gcagtgatcg acatcccgga
    14821 ttacacaccg gaggagaaga agatcatttt ctccagattt gcattgccga aggtgctgaa
    14881 aaggattgga ctgcaggcag acgaatgcac actcaccgaa gagggactgg atgcggtggt
    14941 ggagatctat tccaacacca gtggaatccg tgaccttgag caggcggcgg agcatctggc
    15001 agccaatgcc ctgtatcaga tcgaagtgga cggggtaaag gaagttgtat ttgatgcaga
    15061 aaaagtgaag aaattatttc tataagtaat agaagcaggt agtcgcagga tgcggttgcc
    15121 tgctttttgt gcaaagtcgg gggcttttgc cccggcatca ttatagaaaa acgagtagag
    15181 gagagggaca tgacaaagaa agtattagtg atcacggatg cacagaacga attcatcacc
    15241 ggggcgctgg gaaacaagga gtgcgaggcg gcggtaaagt atatcgttgc ggcagcagaa
    15301 agcggggaat actacaaggt gatctttaca aaggataccc atacggcgga ttatctgcac
    15361 acccaggaag gaaagcgtct gcccgttctg cacggacagg aaggcacaga aggatataag
    15421 atccatccgg atatcgtgaa ggcggtacag gagcattatg cgccggaaca gatccttacg
    15481 gtgaaaaagc ctacttttgg cagtctggat tttgggaata cgctgaaggc aatctgggaa
    15541 gaggtgacgg cagcaggaga ggcagcaggg ggtgaatatc ccatggaagt ggattttacc
    15601 ggattctgca ccggaatctg tgtgttaagc aatgtggtca tggcaaaggc atttgtaccg
    15661 gaagcgagag tgtgtgtcat tgagaaagcc tgtgcctgtg tgacacccca gacgcaccag
    15721 acggccatcg cagctatggg accgatccag gcggatgtga tttagagaac atatcatatt
    15781 tacacagttg tgaatctaag agagaaagag gataaaatca tgcaattacc acagatcatc
    15841 aacagcttac tggaaaatga tatgtacaag ttctctatgg gacagtgtat ctaccaccag
    15901 ttcagcgctt ataagactac ctggaccttc aaatgcagga atacagatgt atttttcaca
    15961 tctgaaatgg tagaagaaat caaggcgcag atccgggcct attgtgactt acgttttacg
    16021 gaagaagaac tggcttatct ggagaacgta aaatggatca agggttccta tgtggatttt
    16081 ttacgtttgt ggcagcccag atacgaggat tttgagatcg gcacggaggc ggactgcggt
    16141 ctgaccatcg agaccaaggg atcctggctg aatacttcta tgtatgagat cccgacgctg
    16201 gctattgtta atgaagtatt tttccgtatg aattatgact atgagaagct gtatgcctcc
    16261 ttccgggagc gtctggcaga caagctacag aagctggtgg acggaacgta ttgccttgga
    16321 aatttcagtg aattcggtct gagacgccgt ctctctgcag aggcacagga actggcagta
    16381 caggagctgg tgggtcacaa taaggattat aaggactccg cctgcattgg tacctccaat
    16441 gtctatctgg caaagaaatg gaacgtgaca ccggtaggca ctatggccca tgaatggatc
    16501 atgtgtaccg ggcagggcaa tcacaagcac aatccggcat attccaactg gtatgccctg
    16561 gatgcctggg tgaaagagta tggcattttg aacggaattg ctttgacgga tacgatcacc
    16621 acggattgtt tcctgaaaga tttccagctg acctattcca ctctgttttc cggcgtgcgg
    16681 catgacagtg gggatccctt tgcatggggg gagaagctga tatctcatta tgaatctctg
    16741 gggattgacc cgaagcgcaa gaccctgctg ttcagtgacg gcctggactt tgcacgggca
    16801 gatgaactgc gtaaggcatt ccgggacagg gtgacggtgg ctttcggtat cggaacctat
    16861 attgccaatg atacctgcga ggaaccgttg aatattgtca tgaagaccac tgcctgcaac
    16921 ggcatggatg tggcaaagat ctccgatact cccggcaagg aaatgtgtaa gaatgcagat
    16981 tacgtggatt atctcaccag atgtatcaga tggagactgg aacatgacag ataggaagca
    17041 gcagaaaatc agaaacggcg ggaatacagc atggatgaaa aaaacagaat agaaaacaga
    17101 atcggtgtgc tggatggaat ccgtgccata gccatcggga tggtagtgtg gtttcatttc
    17161 tggcagcaga catggctgac accgtatctt cggattccaa cagagtacac aaaatatatt
    17221 ggaatcacag agatcaacct tgccggatgg gtcaggtatg gatttatttt tgtggatatg
    17281 atgatcctgc tcagcgcatt ctgcaatttt tatccttatg caagatctat tgttctggga
    17341 gaggactggc cggatacgaa gacattttac agaaagaggg cagcaagaat actgccttct
    17401 tactatttta tgttggcgat cgatctgttt ttctatattt taccgggaca taaatatcgg
    17461 aatgtgggag tattgtgcaa agatattgtg acacatgtat tttgcgttgc accaaattgg
    17521 tcggacactt atatatccac atcttttaac ggcgtgttat ggacggtaca gatggaagtg
    17581 atctattatc tgctgctgcc gtggatcgca agattgttcc gcaagtgggc ctttgccact
    17641 tatagtgtga tgttgatgat ttccgttaca agtaccgcgg tgatcctgaa ctgttttgcg
    17701 ggaaaagaga gaaattatgg aaataatatt ctgacgttta tgggaatcta tgccaatggc
    17761 atgctttgct gcatcctgta tgtcatgtgg aagaaatatg tgacagaaaa taaatactcg
    17821 cggctggcag gaacagtgat ctctgttttg tgtatttttc tgatgaacgg gatgattcat
    17881 aaatacgacg gggatgtgaa tctgcaggca gttcagctgc agagcaggct ggtgcagtcg
    17941 tttctgtttg cattgttcct tttcagtacg gcctgtgcgg gagaatatta tcagaaacta
    18001 tattccaacc gggtggcatc acttttttgc aaattttcct ataacctgta tctctggcat
    18061 cagatgatcg ccgtctggct gaaagaaaaa agaattcctt attggagcgg ggatacgcca
    18121 ccaaatatga caggtgaccg tgtatggcag ttgaaatatc agatactgat tatcgtggtg
    18181 tctgcggcgg tggcggtagc agttactctg atcgtggaaa ttcctgcggc ccgatatctg
    18241 ctgaaagaca gggacagcca taaggaagaa ggaaaacagc atgacaggat atgaacatat
    18301 tgagcacacg tttgcaccgg tgtacgatgc ggattcgctg gtgctgatcc tgggaacatt
    18361 gccctcggtg aaatcccggg agaaccattt ttattacgga cataagcaga atcgtttttg
    18421 gaaagtgatg gcagatctgt gcggggaacc ggtgccggag accatagagg agaaaaagaa
    18481 aatgctactg gctcatcaca ttgccgtctg ggatgtaatc cagagctgcg atattaaggg
    18541 atccagcgac agtagcatcc gcaatgtgga gccgacggat attcgaagga ttctggcgga
    18601 atcacagatc acgcggatct atgccaacgg taacaaagca ggagagcttt accgaaaata
    18661 tcagctgccc ctgaccggca tagaagccac ggtgctgccc tccaccagcc cggccaatgc
    18721 ggcatggagg ctggagagtt tgtgcgaagt gtggcgggag acgatagtac ttttataatt
    18781 tttgaaaaaa cgacaaaaaa tgaaagtact attgacaatt catgagatgt tttatataat
    18841 ttatgaaatg ataccaaagg tatcaaaagc aaaaggggat tatcgtatag aacagaccgc
    18901 gcatctaata aggatgcgcg gtctgttttt atatatggca tatagtaaaa tataatttag
    18961 taaagcatga ttgactaaat tataaattag cattatggta aggatgcgga aatggagggc
    19021 aaagatgttt attggaagag acaaagaatt agacgcatta gatagattat ataaatcatt
    19081 gttgtaatat atggccaccg tgtgggaaaa actgctttga tcaaccattt tattgatgat
    19141 aaaaaggcaa tctattttat gggtgttgag agcaatgaaa agcagaacct ggagaatttt
    19201 agtaaaagta tcatcgagtt tagcagcgga attcaggcag agacctcctt ttcatcttat
    19261 caggctgcac tggagtatgt atttaaactg gcagagaagg aacgggtcat actggctatc
    19321 gatgaatatc cttatgtagc aagagcgtcc aagagccttg cgtctacatt acagatgcta
    19381 attgataagt acaaggataa ttccaaatta atgcttattc tgtgcggatc ttctatgtca
    19441 tatatggagg atcaggttct tgcgtataaa gcaccgcttt atggaagaag aactgcacag
    19501 atgaagatta tgccgtttga ctttgcagag tgctgcacgt acttaaacgg cttatcagac
    19561 gaagataaag catatataga tattcggggg catttgccaa gatatgaaag gatattacgg
    19621 ctatgttaaa aatcttattc atctgccacg gcactccagt tttatgataa tgcatacctt
    19681 cgcagactgg
//
LOCUS       DAWDHD010000032        18923 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_200_length_18923_cov_9.609657, whole genome shotgun sequence.
ACCESSION   DAWDHD010000032 DAWDHD010000000
VERSION     DAWDHD010000032.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 18923)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 18923)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..18923
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_200_length_18923_cov_9.609657"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         5258 a         3844 c         5727 g         4094 t
ORIGIN      
        1 gtatgagctt ttccgggacg gcacgtttct tctgtacggg ggcggcacgg atcatgtggc
       61 caagaacaga caggttttag aggaactgct gttaaaagaa atcacggatt ttacgctgga
      121 agagcggaag ggctgtctgg tgtttgtgga cggggagttc tggggattgt atctggtggg
      181 cagggtaaat acggcggaaa cctttgcccg aagagccggc ggttccccgg aagaaattca
      241 ggtcatagag aaccgctatc cctcccagat cgctccggaa tacggagaac tgtaccggct
      301 ggtgaccgag gggaatactt ccggacacgg aacgtatcag aagatcctgg agcagatgga
      361 tctggaaagc tatctggatt attactgtgc caatctctat ttcggtaatt cgcagtttga
      421 ctctttttcc accactttgt ggcgacgggc aggagaagga gagacgggaa aatggcactg
      481 ggaattctcg gatgccacgg atactctggg acggaataag gtcagtaatt acagtgtgaa
      541 tacttatctg tgtccggggg tggcagaaga tctgttttta cagggattgc tgaaaaataa
      601 ggactttcag acagcgttcc ggcagagaat gcgggaatac gtggaagaac tgacgaagga
      661 aaagacagaa gagtatctga caccgcttct ggaaacttac cgggtggcag tgacagccac
      721 ggcggagcgc tacggcctgc gacagacaga ggaaggatat ctggctgacg gggatacgat
      781 tcaggaatat tttgcctcac ggggagaata tattctgcga tatacggaag agcttataac
      841 gcttgtggat cagacggagg ctcctgacgg agtacaggaa acggtcacgg aaagcgcacc
      901 tgaagaataa agggaaaggg gaccacgtat ggaaacaatg gcagaatatc tggcggaact
      961 ggtaaaggcc ggattggagg acagaaaggc ggcgtctctg ccagagggag tctctgtaag
     1021 ggagattgta aagatttcag aggagaacca tatggattat ctgcttttgg gagccttgtt
     1081 aaaaacaggt gggctttcgg aggtagaaaa agagcttttg cgggaaaaag tactgggaag
     1141 catgcttttt accggtatgc agctgcagga attgaagaag ctggaggaac ggatggaaca
     1201 ggcaaagatt ccctgtcaac ccatgaaagg agcccggatg aaattctatt atccgtctcc
     1261 gtttctgcgg gaaatgagtg atattgacat tctggtccac ggagatttca tggagcaggc
     1321 ggcagtggaa ctggaagcca tggggtatgt cctgcaacag aagatcaagc atcatgatat
     1381 ctatcggaaa gcaccgggaa tcgtggtgga ggcgcacaga gccatgtatg accggacagt
     1441 ggacgagagg cagtatgcct atttctcgga tctgtcccgg gcggtgcgca gaaagggctt
     1501 tctgtatgtg tatgattttg aggcggaaga tttctatatt tacatgatgg ctcatatggc
     1561 aaagcatttt tacaagagag gctgtggtat ccggaatctg gtggacattt atgtgtttct
     1621 ggaaaaattc ggtggggaaa tgaacgcaga ctatctgcaa aagcagtttg cgggattggg
     1681 actgacggca tttacggagc atatggagaa gctggcccgg atctggctgc agggagagcc
     1741 cggtgaagcg ttttatcagc aactgtttga ctatatgcag gggtgtggaa tctatggcaa
     1801 ggatgaaaac ggtatctgga accggttctg cgatgcgcag ccggagaagg gagaaaaggg
     1861 ccgagatgcc ctgaaacgct ggtactggtt ccctccttat gaatatatgg tgttgtatta
     1921 cccgtggctt tcgagaaatc ccgttgcggg aaaatttctg cttccggcag cctggggaat
     1981 ccgggccgct cggggtgtgg tctgcggccg gggaaaatac aagcgggaaa tgctgcggca
     2041 gatcgatgca tcacagatcg gtgtaagaca ggatatctac cgcagactgc agttacattt
     2101 tcattagtct gttccattga aatatttata aggagtgtaa gaaagccatg aaaagtccgt
     2161 cagggtttct ttggaagaac ccatggaaaa aagaaacctt acttacctat tattattcgg
     2221 cgatcttccg catgcagctg ctgctcctgc cgccggggaa actgcggaag agctggggca
     2281 gagaaaacga agaatccgca ggaacggcat cccgggaagg ataccggcag gcggcgcgta
     2341 tcgcccagat cgtgggaaag gtctgtgata agactccctg ggagagtaaa tgtctggtgc
     2401 gggcgctggc ggcccagaga ctgttgtgcc ggcggggact ttcttccacc ctgtatctgg
     2461 ggtgtggcat ggaagaaggt aaaatggtgg cccatgcctg gctgcgtttt ggggaaatgt
     2521 atgtgaccgg aggggacggc ggcggatata ctaccgtggc ccggttctgt aaggaggctt
     2581 ccggaaagag gggataagta tgtcggcaat ctggggaatt gtggatctga gtgtggtgca
     2641 gtcagaggca caaagaaaaa atcgtgcagg gaacctgtgg gaagaggctc tgcggatgcg
     2701 gcaggcctat aggacgagct gcctggaccg gatacaggaa aaaagggagg caacctatta
     2761 tctggcctgc ggggtacaga atgtcacaag ggaagctgtg gaagaaagat tcccttatga
     2821 aagaaaggga gagcgcagaa gtctgttcgt ggcggatgtg atcctggata accgcggaga
     2881 actggtgcag cggtttggag gcatccggga tctgtgcagc catccggacg gggaaattct
     2941 gtatgaaagc ttttgcagcc atccggagga aacgcttgcc gtggccagag gagcctatgc
     3001 ctgtgcgtat ctggagccgg ggaaacggac cctgactttg ttcaatgatg cggtgggaaa
     3061 ccgttcggtc tattattttc aggaggaaaa aagggtctat ttttccacgt tgcttgcggg
     3121 gatcacctgc gaacgggaga actggaagga aaatacgggc tggtttgacc ggttctatac
     3181 catccgggat ctgcgggcgg tgtcggaacc cagagagaca ccctatgcgg ggatcctgcg
     3241 actggctccg ggagaaattg tggtatttac tgaagaaggg gtgcacagaa gggattactg
     3301 ggatcccttt gcgggaagga agatcctgcg tgggaagacg gaagtcgggt accgggagct
     3361 tgtgaccacg gtgttccggc actgcgtgga ggatgtgatc cgggaaggcc ggggtggaaa
     3421 agaaacagga attctgttaa gcggcgggct ggactccaat gcggtggcag cttatgcagc
     3481 accttacctt gctgccagag gaaagaaact gtattccttt actgcggtgc cggaggagaa
     3541 ggaaagagca cggatcccgg gacaatacgc tgtggaggat gagagaggca gtgtggaact
     3601 ggtgcggaga tttcacggga atctggaacc ggagtatctg gtgaccaacc ggggagatct
     3661 gctggcggag aaccggaggc tgtgggaagt tctggaagtt ccctgtaaag cggtactgaa
     3721 tctgccctgg atgtatgaaa gttatcgtct ggcagctcag aaaaactgtg ggatcctgct
     3781 gtcgggacag tacggaaata ttaccatatc ctatggggat tttcgaagcc tgttcctgac
     3841 actgctacat cagggaagaa tcaaagaact ggtgcgggag atcaatgtct acagcagaaa
     3901 ataccgcagg agccgcaagt ggatctggcg ggatcttctc acggcggaag ccggtgggga
     3961 tcatgaagcg gtgtcccggt atatgtatga caaaagtgcc ttgcggcaga tcggagaata
     4021 tgaagtaaag ctgtctctgg ccaccggagt ggtgcccaga gatcccacca gagacaagcg
     4081 gctgatcgcg ctggtgctaa gtctcccggc ggaacagttc acccatgccg ggcaggagag
     4141 acgcctggtt cggcagtatt tacaggggaa gatcccggaa gagattctgg cggatgaatt
     4201 ccacaaggga agacagggcg tgggaagcgg ggaactgctc gccttgcagt gggaacggat
     4261 ctgtgaggaa ctgtcgggga ttccgggtga gacgatgtcc ggggaaaacg ggcactcaaa
     4321 agcagacatg gtgcgtggct tttatgtggg cctggcacag gaatatcgga gaaggtttga
     4381 ggtatgatgg aatattttta cagaatctac ggactgcggg tgcagtcgca gatccccttt
     4441 tcggaggcag taccggagac ggcgggagag gcagaggtaa aaattgtatt cggaaggatg
     4501 ccggatttcc tgttggaagc gggacagaag ggctacggga cctggacaaa cggctttgta
     4561 tcggcctggt tccgcatccg ggacggaaca caggtctatg tggaaggggg cagtaggatc
     4621 accgttgagc tttcggagga accgcaactg cttgtgatca cttccctgtt gctcagtgcc
     4681 gggatggcac tggtctgtct gcagaggggg gaaccctttt ttcacggagg tgcattgtat
     4741 accggggaac aggccatttt actctgtggg gagagcgggg ccgggaagtc tacggtggcc
     4801 atggaattgt tgcaaagaaa gcttggattt ctggcggatg acacagtgag ggtgcatctg
     4861 ggaactatgg ggatgctggc ggagccttcc tacccgcagc agaagctttg tcgtgatatg
     4921 gcgctgaaat gcgggaagcc gctggaagaa ctgatctata ttgacgaaga gagggacaaa
     4981 tatgcctggc gcagacagga ttgctatcgg aaagaggcgg cgctcctcgg gaagattttt
     5041 ttgctgcgaa aggatgctgt ggccgggtgg caggatgcgg tgcagaatac cggggaagag
     5101 gcagtgagtg tccaaaagct caccgggcag aaagcactgg agactttgag cagccagttg
     5161 tatctggcgg atacctaccg gtacagtacc gggattccgt atcctttgat ggaacagttg
     5221 gtccggatcg cagggcaggc cggaatctat gaagtgatca ggcagtcgga caaggataca
     5281 ctccacgagg tcgtaacgaa aatcttacag ttttgttaga aagagggtat ttttacaggg
     5341 aaattattat aatcagaaat gtaagaaatc ataaaaatgt aataaggagg aatggaaaat
     5401 gaaagcatgg agtcagcccg aaatggagga actgaacgta agagcaacgg cttacagccc
     5461 taagggaggt ctgatcgagg acggcgttta ccagtccgat gacggtaagt atactatccc
     5521 cacttacggt ccttcttccg gcaattccgg agaacctacc gtagacgtac agtagttttt
     5581 ggactgtatg agaggaaaat gccagaatgg atcagggaag ctcatggtta catgggcttt
     5641 tttggtgtat atgatgagtc cgcctgctcg attaacagac ggagacagag cgtgtaaaag
     5701 aagggagaac agaaaatgga caaaacggca aaaatccgaa tgaaagcgaa aaggaatgtg
     5761 acggacctgg cgggagaaaa ggtcatggtg gatttcgaac aggggaaata tttctgcatc
     5821 aaaggtgtgg gaaacgacat ctgggatatg ctggacgggc aggaaggcga agacggcata
     5881 acggtggagg ctatcttaca taaactgatg gaagaatatg aagtgatgga agaggtgtgt
     5941 gaaaaagagg tgctggcatt tctggacaaa ctggaacagg cgggaatcct ggaagaggtg
     6001 tagacacata tgggagaaat aagaggaaga gattatattt cctggttgtg gcaatatacc
     6061 gctccctatg cgggcaggat tggtctgctg ttattgtttg ggctgttgga gacttttgcc
     6121 tcgctggcca tggtgcagat caccaaggag atcattgatc atgccacagt gggaagcggt
     6181 ttcggacggc tgcttacggg ttatgtgctg ctgttattgg gaatgcaggg aatctcggtg
     6241 ttgtcgacac tgcttactgc actggtaacg gagcggtttt ctttcgggat ccgacggcag
     6301 atctatgaga agatcctgca ttctggctgg cgggaagtga cggcctatca taccggtgat
     6361 ctcatgacaa gactgaccag cgatgcggga aatgtggcgg acggtattgt ggggacgatc
     6421 cccaatatct tacagctggg gatcgagttg gtactggtgt tttgtatgtt gttttattat
     6481 tccccgctcc ttgcggtgtt tgccctgctg gtggcaccgg tggcagccct gtgctcctgg
     6541 tggctgggaa aacgcctgaa acggttacag cggaaagtgc aggagagtga gtcggaatac
     6601 cgttcctttt tgcaggaaag tctggccaac cttctgatcg taaaggcttt taccggagag
     6661 gaccgggcgg tggagcgcct gacaaagctg cgggaggaac ggttctactg ggtataccgc
     6721 aagaccggac tgggaaccgc cagttccacg gtgatgtcac tggcttttca gggaggatat
     6781 ctgatagcat tttcctacgg ggcatggcag ctgtcgagac aacagatcac ctacggtact
     6841 atgtcggtat ttctgacact ggtgaaccgg gtacaggcgc cgatcctggg actggcccag
     6901 cagattcccc ggatcattgc cctgttgact tccgccggga ggatcatgga gttacagaga
     6961 attcccgggg agcaaagggc agccggagag atccgtggat gggagatcgg agtggagctt
     7021 cgaaatgtat gcttcggata taccagggaa ccggtgctga aacgggtgaa tctgaagatc
     7081 tgtccgggag aatttgtcgc cattgcagga gagtccggta tcggcaagac aacactgatc
     7141 cgcattctga tggcctttct ggaaacggga gcgggaacgg tggatttcta caatggcagc
     7201 ggacagaggc aggagaccgg agctttcgtc cggaagttta ttgcctatgt cccccagggg
     7261 aatactctgt tcagcggaac cgtgagagaa aatctgcaga tgggcagaga agatgcttcg
     7321 gaggaagaat tgtgggcggc cctgaaactg gcttccggag aaaattttgt ccggtcactg
     7381 ccggaggggc tggataccgt catcggggaa agagggcatg gcctgtcgga aggacaggcg
     7441 cagcggatcg ccattgccag ggcctttttg cggagagcac cgtttctgat cctggatgag
     7501 gccacctcgg cgctggatga agctacggag gtcgccgtat tgcaggggct ccgggcatta
     7561 gaaccgagac ccacctgcct gctgatcacc cacaggaagg gtgttttaaa atattgtgac
     7621 agagaacttt gcatccggga agggagtgtg acggaagaat gcagtaccac tactatctgt
     7681 acggcatgag ggtaacagcg gatctggact ttgtacagct ggatccggtg cgtttaccgg
     7741 aggatgacag ggacttcgga cgggaaaagg tccggatccg ggcgatggac gaagaggagg
     7801 aagcacagtt tcggaaagaa tacggagact gcgtctgcgg aagttttttg ggtacagaaa
     7861 aaggctggtt gtgtaatcgt acgctggcca tgagtgtgga gcggggacag gtgatccggt
     7921 atcacatccg gaagggagga gatcccggag cggtacgaac ctatctgctg ggctttggaa
     7981 tagcaatgct cagccttcag cagggaaaac ttcccatcca ttgcagtgcg ctggcaactc
     8041 cggaaggtgg tgcgatcctg atcgccggag agagtggtgc gggcaagtcc acactgacga
     8101 cagaactgct gaaacggggg tatcgttttc tggcagacga tatggcagtg gtggacattt
     8161 ccggaaaaga aggggtgtgg gtgtatccgg cttttcctta tatgaagctg tgcagggatg
     8221 tggtgctgag gcagggatat cccccggaag aacttttgta tattgatgaa aaaaaggaca
     8281 aatttctggt gccctgtacc ggggtatttc aaagggaaaa ggccagactg gaacgatttg
     8341 tttttctggg aatccgatgg aaaaaagagg cggaagataa gtccgggaag ataaaagcag
     8401 aaaaaatcac aggccccgac agcatgatcg tgtataaaga caatctgttc ttaagacatc
     8461 tgtggaaaaa acaggatccg ggaatggaaa tctggcagaa ctgcctgaaa atagcggagc
     8521 agataccggt cttttggata aggagaccgg cagcaggtga ttccactgcc gaggtggcag
     8581 cggaagtaca cgcactgcga aacgtcgtgt cagcttaaga actcttccgg ttcctcagaa
     8641 ggctgcgggg gctgacagtc gccgccaccg ggatggtgtc cgggatgatg tcctcctccg
     8701 ccgggatgtc cggggtacca gggaaaccat ccacaggcgg tctcggatac cttcagttcc
     8761 atcagttcag gttgtgtcca atgcttcata agaattctcc tttccgtctg ccttatgcag
     8821 agatgtacac ggggaaagtc cctgtgtgac tacagcatag ggaacacagg ggcggaagta
     8881 aagaaatgta tcatgattgt aagaattttg caggaagtgt cacagaaaag atactgcctg
     8941 caccgggggc attttcgcag gacacggttc cgccgtgacg ttctgtgatc gtctttacaa
     9001 tggccagtcc cagaccactg ccggtggcag aaccgggagt tctggcggag gagacggtgt
     9061 aggtgcggtt aaagatccgc tcgaggtcct ccggcggaat gccgggcccc tcatcggtca
     9121 cggagatccg gagagaagta aggtcagcgg aaggcgcggc agacagagtg attttcgttc
     9181 cggcgggagc atattttacg gcattgccga gcagattgtc cagaatccgt atcagttttc
     9241 cgggatctgc aaaaagggtg gcatggagat cctgcgatac agataaacag agaagatgac
     9301 cggcatagtg tctgtccgga agtgcggtat agaaatattc ttccagaaag gccggggcat
     9361 ccagttcctg aaaggaaaag gcgtgggaag gatcctccag agtgaacaac tcatacatat
     9421 cccggatcag atgttccagt gtcccagtcc ggtgatcgat cagggtgagg gcaccttcga
     9481 tatcctgtgc agacagagac tgtccggaag taagataatc cacggcactg cggatggcag
     9541 tgaggggagc ccgcagatca tgggagagat tggccagcat ttccgtacgt tcctgttgca
     9601 ggtgctgcag ggagcgattg ctggcattta actgcgcggt gatttccagc agcctgcggg
     9661 acagttcttc cacggtctcc tgttcgagag agggagaacg gaaaacgggg cgggaatttt
     9721 gtgccatggt cagatacctc ctttcagaaa acgataccct ttggaccaga cggtctcgat
     9781 gcggttctcc aattcgtaat tgccctcgaa acgtttgcgg agacggctga cattgaccat
     9841 aacgctgcgt ctgtcacttt cccggtagac accaaagaga gccgttccca gttcctcgaa
     9901 ggtgatgtcc cggtccgggt gttctgccag atacagcagg acttcatatt cccggttggc
     9961 gagacacaga tcctgttccc ggtagaaggc cttgtgggct accctgtcga tggaaagatt
    10021 tccgatcgtc agtaccagat cggaagaagc ctgcgccagt tgtctgcgcc tgcggatcag
    10081 gaggtccacc cgggctttca gctcccggag actgtagggt ttgaccagat aatcgtcccc
    10141 gccgcaggta agtccccgga tcacatcttc ctcggaactt ctggcagtga gaaacaggat
    10201 cggcacagag gaaaaagagc ggatgtaacg gcacacccgg taaccgtcgg cttcgggcag
    10261 cataacatcc agtaagatac aatccggaag attctttcga atggataaga cgccttcccg
    10321 gggggagatg gcagtataca cggtgaatcc cgcaccggtg agatatttct gattgatcgc
    10381 cagcacttcg gcatcatcat cgatcagcac aagcttgaac atagtattat cttacctcct
    10441 ttgctggtgc tattttagca tgggggaaaa acgattgtca ccaaaggaag gtcgacagag
    10501 agagacaaag gacagcggaa aacgacagac cggatgaaaa agtgacacga gaagggggag
    10561 aaaaatgaat cggtcaaata cgagtgcaaa taaggatatc ggacacatag taaaggatat
    10621 gctgcggctt ctggtggctt atggagtggc tgtgttattt atgggaagag catggagcct
    10681 tcggacactt gggggtatga caggactggc agcgatattc gtgctgcttt atgtattggg
    10741 aaaggaagag caatatctgt ataatgtgac cctgttcggt tatccggaca ggatacagcg
    10801 gcgcagaaca ggatcgtttc tggtggcaac agcggcaacc gtggtcctgc tgccatatat
    10861 tacagaggat cagaggggat attttctgtt tctgatcctg gcgtacagtc tggaaagcgt
    10921 accgttgctg agacacggca gatgctgcag agacatggaa agaaatcgcg gggaagtacc
    10981 gcgtaccgca tttgtgggaa gaagggcgca gtacaacaag tttcggtatt ttttgaagaa
    11041 gaccagtatc cccatggagc ttgtggggta tattgcggca tcggcacagg aactggcaca
    11101 gatttcagaa ggagatgcag gagaatatct gggaagtctg gaaaatctgg aaatgctgat
    11161 ccgccggtac catctggatc agatctatat tctgcaaaag cgcgaggaac agcttttttc
    11221 catgcagaaa tatgtggatc tgtgcattga tatgggggtg acggtacgga tggtggtgga
    11281 tttctataag aggcgcagag ccgacagtta tgtaagctgt gtgggaacct atccggtcat
    11341 cacctaccat acggtgacgt tgaataccgg agaacaggtg ataaaacgga tcatggatgt
    11401 ggcaggaggc ctggcaggga tcgttttgtt ttctccggtg atgttattgg cggcgctggc
    11461 cattaagctg gattcccccg gtcctgtgat cttccggcag acccgggtcg ggaaaaacgg
    11521 caggacattt cagatctata aattccgtag catgtatgtg gatgcggaag agagaaagtc
    11581 agagctgctg tcgcaaaatg agatcgccgg gggcgtaatg ttcaagatcc gggaggatcc
    11641 ccggatcacc cgggtgggca ggatcctgcg gaatttaagc attgatgagc tgccacagtt
    11701 ttttaatgtg ttgggaggtt ccatgagtct ggtgggaacg agaccgccga ccccggacga
    11761 ggtggagaaa tacggcacaa ggcagtggcg gcggatcagt atcaagccgg gcttgacggg
    11821 catgtggcag gtgaacggca ggagtctggt gacggatttc gaaaagatcg tggagctgga
    11881 tgtggaatat attgataact ggtcactgat ggaggatatc cggattttgt gcaagacggt
    11941 gacagtgttg ttaaagcatg cggatgcgta ttagaaagag aggcagaaga ggtggaatga
    12001 agatgcatgg aacccgggtg aactgggtag gaatgaccgg tgccgtggta ttgtttggat
    12061 atggatggtg gagagaaccg ttggcggcgg tgctgggagg gatgatcctg cttgcggatc
    12121 tgctggccat ggaggtgagt gtaaggaaaa gggatgggaa aaatgctaag aatacaatgc
    12181 tttgaaaaaa atgtaaaaaa aaagaaaaat atttctgttt ttctgaacct tttttctgag
    12241 acggaaagtc ttaatagata agacagaata aaaggggcgt tttaacaggc atctctggaa
    12301 gaaaggagca aaaggggaca tggaagaacg aagacggatc gaccgtgtgg gctaccaggc
    12361 aaagagtgtt atcgtggtat gtgataccgg ggaaagcatt tttgtggaga cccggaatgt
    12421 cagtccgctg ggcatagcct ttacgatgcc ggcaggatct cctgacctta aggggaaaga
    12481 catcataatc gtagcggata cgatgatcat gtatgcagat gtcaccagac aggaggaaca
    12541 agaagacgga aattttaaag tagctatttc ggcaaagaag ttcacgccgg aggtgctgca
    12601 gtatttattt gaacatattg ttgaaaaata gaatggagag gaaaaatcat atgagaaaaa
    12661 acagtaagaa cgaaaaagtg attcgtgcta tggcaatcgg catttccgca atgctgatgg
    12721 catcttcacc gttgactgca ctggcagcag aaggagaggg gaattcttcc gaaggaaacg
    12781 aggataagaa cattaccgtg acaccggaag ccggtattgc tgaccaggca caggcggcag
    12841 caaaagaagc tgacaaggca gtagaaacgg cagaaaagag tgctactgat gtgaagtcag
    12901 aagtggctga tcaggtagtg gcaggggaag ctaaggatac gcagggcaag gatctgtctc
    12961 aggctgttct ggatgccaat gccaaagtag aggataagac cgtggagggc ggttcttctc
    13021 tgaaggacgc agagtctgct gtggagagtg cagataccaa acttggcgtt gcagaagcaa
    13081 atgataagct gtccgacgca gaactgaata aggcagctga tgctgcggca aatgcaggac
    13141 agactgcggc agacgcgaag gatgccatgc aggctgccca gaataaggta aatggacaga
    13201 tcgagaatat caagggtgct gcttctatca ccgatgccaa tgcggcatat gaagaagtaa
    13261 agactaccgt ggatcaggca caggctgatt tcgatgcaaa gcttggggag tacaataccg
    13321 caaagacagc ttatgaagag gcagcacaga aggtggcaga ttatgaaaag gcttatgagg
    13381 aggctgtaaa cagtgcagat gctaacgctg cggcagcagc agctgaactg gaagcagcga
    13441 agacaaatgc agaagcactg gcaaaagctc tggaggctgc aaagggtgca gtggataagt
    13501 ctgcagcagg tgctctggat attgcagata aggaaactct gacccaggga gacaatggac
    13561 tgaactggaa aaatgaggat cagcttttca tttccattat gcagaactac tatctgcccg
    13621 aggtacagaa gatcactgcc gatgatatta aggttgtccg cagacagggt gaagacaata
    13681 ataccaagaa ttattttgaa gtaacctata ccgatgagaa tggtaataag cagacaaagt
    13741 tttacaacta tgtgatggat gacaaacaga cttccaagga caatatcgtg atctttgaga
    13801 agcgaattga ggaagtaaac tggaagacgg cgcaggagac caatccggat cagtatgtga
    13861 aggaaaatgg ggatactatc actgtttccg aagtggaaaa aggtctgaaa gacggaacca
    13921 ttattgcagt agatggaaag aaagtgatca agaacgacgg aaccgagagt atcattattt
    13981 ctgacaataa ccagaagacc gaaaacggcg aggttgatac tgatgtaaat gaggctaccg
    14041 agaaggaatc ctggaaactg gatgagaacg gcaacctgat caagaccgtg accgctgatg
    14101 tgaccaccat cacctatacc gatgcaaaat tcacttctac cgaacagtat cagacagagg
    14161 cagagagaga tgctgcagct gctgcaaagg aaaaggattt gaaggacgct gccggcaagg
    14221 atgtaaccgt tacaggaaca gaaaaaacgg attatactta taccggtaac ggaacctaca
    14281 ttcctacctt tacgaagact gtgaatgtga aggatgaaga ggttgaatgg aagcatacag
    14341 ataaaaagac ggattatggt gtaagaacag aggaagaagc agtagcaaag gttacgaaag
    14401 agcaggagaa ggcgttaagc aataagatta acgatgatga cgacttatac ctgatcggtg
    14461 taagcagtga tcttaaggta accggctata cagaagatca ctggtatgat gattccgact
    14521 tcctcgtatc cggcactgta tccgcaacct acgcaaaggt taccaagaaa actgtcgatc
    14581 agagtacctt cggttctctt tggaatgata tcaaggctct gtttggtaat ggagaaacta
    14641 cgaacaagaa actggaagat gcagccagaa aggcagtcga ggcagatggc ggtatttttg
    14701 taagtgccaa ctgggatgac tggaagcttg gcaaggcaac catccgctat gtagcaggtg
    14761 tcagtgtcaa gaccgatgag aagaccaccg cagcggaggc acagaatgcg gtacaggatg
    14821 cagcactggc acaggcaaaa gcaagcggcg caaccggtgt gtacaatgtg aagactacag
    14881 ataccgatac cattgctcat accagctatt cctatgagat tgattatctg gagaaaaccg
    14941 gagagaccac aaccaatact gctgtcagaa ctgagactta tgcaaatgca gaggtgctga
    15001 ccggtcagat catccagaac ctgaattaca ttcagggcaa catcaaactg acccagaagg
    15061 atactgaata tcgtaagttc gtagatgatg ccaaggctct gacccaaaag tatcagaagc
    15121 tgttacagga tgcacaggac gcacagaaag acgtggaaac agcgcaggct aaggtcaatg
    15181 agctgaaggc agagatcgaa gctctgaaga gtaacagaac cagtaacctt ggagctctga
    15241 aggaactgga aggaaagctg gccgtggctg aacagaacaa aaaggatgca gaagatactc
    15301 tgaaggagat tctgggcagc ctggatgagg caggcggaga gctggataag gtgatcgagc
    15361 gtctgactcc tgcacctaca cccggaacac ctgcaggtgg agaaggtgag accggagatg
    15421 ccggtgatac agaggaaggc ggcgccggag aggcagcaac agttgttaca cctgttgcac
    15481 tggcagcagc acccgcagca caggcaaccg tagttgcaca gaaccaggca gcagcacccg
    15541 ttgtacagat cgctgacgaa gcagcacctc ttgcagaagc agctcctgca aatacacagg
    15601 aaaccgttca ggcaggttcc gataaggaag agaccaagga agccgtaaat atcgaagaag
    15661 aggctgttcc cctggcagat gtagctgtgg aaagcgaaca tgcaaagatg tcctggtggt
    15721 ggtggctgat cattctgatc ctcggtgcaa ccggatatga aatgtacaag aagcacaatg
    15781 agaagaagct gaaggctcag gcagaaaatg caggagatat cgaagaataa aagataacaa
    15841 aaacgacatc catcgggatt ttcccggtgg atgtcgtttt ttagaccttg ggaagcggat
    15901 tactcgaaga gctcttcaac tttttccaga tcaccgtctc cggtcagaag aagagtcact
    15961 gtggcctggg cactgctgat atcggttgtc ccctcaacgc ataattcacc gtcagtattg
    16021 tagagagccc attggaactc attgacagag gagggccagt ctgcttccat agagatcgat
    16081 tcctgatccg acagggaatc cagatttacc tgttccccgg tcacgggatc aatgaccgct
    16141 accataccga cttcgatgcc acacatattg actactgaca gagtgagtgc cctggtatcc
    16201 tccgaagtgg attctatggc agacgactcg gaatccgggg aaacatcggc aggcagggcg
    16261 gcaacagtct cagacgggga ggtggaagca gtcgtggcag atgatgtttc ctctccgtca
    16321 gaattctgac tgcatcctcc gaataacaga caacaggaca atatacttac agcaaaaata
    16381 tgtcgtatga aacttttttt cataataatc tccattctgc agacgattcc ttccgtcgga
    16441 gaaatcgcga atcaaatttc aaatttgatt ctgcgataag ggctgctttg tgacgcctgc
    16501 agcacaaata gccgtgtgaa aatctatatc accattatat cggatttccc ataaaaagta
    16561 aatttatgcg ataaaaattt ccggaagcag accggtagta attcctgaaa aaaatatcga
    16621 aaaatgtgag ccgtttttca tggataagta gtctttatag atagagacta agaaataacg
    16681 gaggcggtcg agatgagcgg catgttatat tccatacctg tgattttgac aggttatcga
    16741 atcgtacaaa ttgaagatga agcgacaccg cttgcacagg cagcaaagag tggatattac
    16801 ggtgacacag tagccggaat gctgggcatt ggtgttttat tactcttggt gatttatttt
    16861 atattatgca gaaaatacca gaaaagaatc atagaattgc aggaaaatgc acatcccggc
    16921 tggaactggt ggaaacttag aaagcaggta accgaactgg aggataaaaa ggcagaagga
    16981 attgtcgagg atatccggcg ggcagcgcaa agctatatga ctgtttaaat ttaatcagaa
    17041 agatccgggg atcatccccg gattttttac accagattta taaaagtgaa gtatttcgtt
    17101 gctgatgtct gccgggaatg ttataatcat gtatgtggcc ggttcagaga cctgcgccac
    17161 aagcatagga aagaaaagga aggcaatcta tgaaagtctt ggtaacaggc gtaaaaggcc
    17221 agttgggata tgatgtggta aatgagttga caaagcgcgg aattgaggct gtcggcgtgg
    17281 atattcagga gatggatatc acagatgcgg ccagcgtgga aaaggtgatc ggagaggcgg
    17341 caccggacgc tgtggtacac tgtgcagcct ataccgcagt ggatgcagcg gaggataacg
    17401 tggaaatctg ccgtaaggtc aatgcggacg gcacacagaa cattgcgaat gtctgcaaga
    17461 agctggactg caagatggtc tatatcagta ccgattatgt gtttgacggc gggggcacca
    17521 gaccctggga gccagaggac gagcgtcatc cgctgaatgt atacggacag accaaatacg
    17581 aaggagaact ggcagtacag aatacactgg agaaatattt tatcgtaaga atctcctggg
    17641 tattcggagt caacggcaag aacttcatca agaccatgct gaatctgggc aagacccatg
    17701 atcatctcac agtggtgaat gaccagttcg gatctcctac ctatacttat gatctggcaa
    17761 ggcttctggt ggatatgatc ctgacggata aatacggtat ctatcatgct accaacgaag
    17821 gtatctgtaa ctggtatgaa tttgcctgcg agatattccg ccaggctggc atgaaagtgg
    17881 atgtggctcc tgtaccggct tccgagtatc cgacgaaagc caagcgaccg gagaacagcc
    17941 gcatgagcaa agccaagctg acagagaacg gattcgtaag gcttccttcc tggcaggatg
    18001 cgttagagag atatctgaaa gaaattctgt aaacagattt tgtgtaagca ggaaaataac
    18061 gggaaatact tcagggagcg gtatcggaaa cggtacggct cccattttgg cgaatgtaca
    18121 tcatcgagct tttaagcgag atggtgtaca aagtatacaa atcgagtagg agccagccta
    18181 ctcgacttat aatatatgaa aaagtcatga aaaaccctgt aaatttcacg aaaaacctgt
    18241 tgacaacgga agtcggaaca agtatactgt taaagcgtgc cagaaaaggt gcgtctatta
    18301 tactattaag aaagaggtga aacagaatgc caacatttaa ccagttagta agaaaaggac
    18361 gtcagacttc tgttaagaag tctactgcac ctgctctcca gaagggttac aactctcttc
    18421 acaagaaagc aacagacgtt tctgctcccc agaagcgtgg tgtatgtacc gctgttaaga
    18481 cagcaactcc taaaaagcct aactctgctc ttagaaagat cgccagagta cgtctttcca
    18541 acggaatcga agttaccagc tacattcccg gtgaaggtca caatctgcag gagcacagcg
    18601 ttgttttgat ccgtggtggt cgtgttaagg acctgcccgg taccagatac cacatcatcc
    18661 gtggtaccct tgatactgcc ggtgtcgcta acagaaaaca ggctcgttcc aagtacggcg
    18721 ctaagagacc taaggatgct aagaaataag tagtatccgg ggcgttttga acgtcggacc
    18781 gagagtccga tgaaaatccg caaggatttt tggagatgtc agtgagacat ctttgtacca
    18841 caaaaacgaa actaagaaaa gtgttggaat tctgtgatac agatataaca gcgcgaacac
    18901 ttggggaaac acatgtgagt acc
//
LOCUS       DAWDHD010000033        18729 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_202_length_18729_cov_11.394452, whole genome shotgun sequence.
ACCESSION   DAWDHD010000033 DAWDHD010000000
VERSION     DAWDHD010000033.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 18729)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 18729)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..18729
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_202_length_18729_cov_11.394452"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         4314 a         5222 c         4147 g         5046 t
ORIGIN      
        1 tgtcgcagca ggttaggtcg ttggcttggg cggggtgccg ggattctggg tggccgcagg
       61 tttctgaata cttccaccgg tcttgtctcc cgacacggtt ccgctgccgg tgatcgtctg
      121 cccatcgtta ccggctgtac tgttgccatc tgcactgccg gtgattttgc cggtaatgct
      181 tcctgtgatg cttccggatg cagtggtatc atgtttttct tcataagccg caaaaggatc
      241 ctgttttgcc atttctctga ccgcaccggc gctgacccag tattttccgt caaaccattg
      301 tacttctccg tcaaaaatac ggatctgcgg ggtatcactg tctatggcac ccgtggtgtt
      361 gtccgctgcg ttgcctttct ctccgccgtc cgtggcggca ccggttccct ctgctgtatt
      421 tgcattgtcc tctgctgtca tatcttctgt cacctcatag gacgtgtatt ccggacgggt
      481 ctccagtccg atcatttccc cgcaaaaacg atactcctct gcggtaatat ccccgccgat
      541 tccctgccac aggcagttct caccggtagc atcataggca tagaggatca gatccgtata
      601 atctgttccc tccagcagtt ttttcttgat ttcttcggag ggctgctctg tcaggatctt
      661 tccttcggaa tcaaaacttc ccgtgtaagt gatatattcc ataccctccc ggttgctcca
      721 gagggaatag acatccagcg caccttctcc ggtatggaca tcacaggtat agtctcccgt
      781 gagcacaccg ccctgcatgg ttccctgttc atgggtaatg ctcccggtag cgctgtccac
      841 gctccaggct tcgaaggtac cgttccagtc cttcatggtt tccggtgtat ctgccagagg
      901 cgctgttgta tccgaaatct ctgtcgttgt attttcccgg gctctggctt ctgtggttcc
      961 cgtttcctgt gtggcatcgg gattctcagt ttctgccgga tccgcgatgt tctcgtccgc
     1021 accggccgct gtcactgtta ccagccggat cgttgcaccc tcctggctca gaaaacgttt
     1081 cgtgtcactt ccggtatacc acaccctgga gaagcattgt ccctcttccg tatagccggt
     1141 ctgtacatag agcagaggct gactgtctct ttccagatag tagtttcgct gtccttcttt
     1201 cagtttgggc atcatggtgg tgaaccagtc ctcgctgtcg atgagattca cgctctcact
     1261 cagatactcg gtcagctcca gatcattttg catttcctgt gcacggctgc ggatcccctc
     1321 tgtctcttcc tccagattca cactgagctc ctgtagagca gcaaatgctt cctcatccgc
     1381 atagagccgg taatcctgtt ccagcatatc ccgctgtttg cggatgtatc ccgctctgtt
     1441 gtaggcatcg gccaatgaca gataatcctc ctgggtaaac tctcctttat tatattttgt
     1501 ctccagattg agaatctcca gctccagctc tgagagcatc ggctgtgcct ctacgggagt
     1561 cacggcatcc ttcgcatttt catcactgcc atctccgcag gccgtcaggg atgttaccag
     1621 aacagcgctc atggccagtg ccagaaatct tgttgctttt cttttcatct ttttgtccta
     1681 tcttctatat tctatgatgt cgagaccaaa tgctctcgac attgatacct gagaatcatt
     1741 ccgtgctgta cgtttccact atcctgcccg atattcgcat atcattcctg tgcatcgctg
     1801 atgagcttac gcaggaaagt atccgcctcc gatccctgtg cataggttcc catattcagt
     1861 acctgctgtg ccagagcata gatgctgcgg gcctgcaccg gaccgtaaat ggtaatgtcc
     1921 ttgccatcct tgttcaggat gatgtatccg cggaatgcat attccacctt atactggttt
     1981 gccggaaggc cgaccagtac ggaagtgaac cggtatctgc cactcacggt ctcataaata
     2041 ctatcctgat gggtgccgtt ggcattcgta ccgtaagcca gtccgctgat gactttttcg
     2101 ccgcctttga tcatgggata gcttgtccgg ttggcattgt tcatgaccag tgttccgtat
     2161 tccttgagat gatagccgtt cacaccattt cccagcagtt gtgctctgag gtccgtactg
     2221 ataccggtct taaagcggat tcccgctttt ccggtaatcc ggatggaaaa tccatggtag
     2281 gtcagcagat ccgtaagtcc cggctgttct gtggccacat aggcattgtt acgatattcc
     2341 agggtccaga catacatgcc gataggcgct ccattctcat tataccggta cacgaccgcc
     2401 gactgggcat ttccgttcgg cagtttgacg atacggcggt tattccgcac ctgtggtgta
     2461 taggcaatac cgtccacgta gatcgtggaa gcatcgtaac ccgacggtac ctgcaatacc
     2521 acattggtag aggaagtcgg catcgggcag gggctggccg gcatattttc atagaccgga
     2581 atcttgaaga caaaggtatt ctccagcgct cccgcacttt catacagttt cttcatgctc
     2641 agtgcctcac tggtgggtgc tgagatgttc tgcatatact gatgggtata taccggattg
     2701 ctggcagtgg gggatacatt gaatttctgt aaatacaggg tatcctggcc cttacgtatg
     2761 tagcttgcgg agattacttc cgctcctcca aggatcgagt agtaggctcc atgccagtca
     2821 tgatctttgg cataattcaa tccattgcgg atcacttctt cattggtact gccggaagct
     2881 cccacattga aataattgta ataatgttca taaccgggat aggtgccgga gatcagcggg
     2941 gatgtcccct ctccctgctc ctgcagtact ctggctgcca gatggaacgg gctgacctgc
     3001 cggttctcct ccgcaccgat ggaccagaaa atatggtaga atttcattga ggtctccggt
     3061 gcgttctgac tgctgttcat gaaggttccc tcgaggaaat tctttaccgc ttcttccgta
     3121 tgatagcttg cgttgtaggt cagctgttca aactggaaaa tagcattctc ctgcagggaa
     3181 tttctggggt ccatgtataa tttcagtaca tcttccgaag cgaaatacca gtttgggctg
     3241 tgctgtcctt ccttgcaata atccccgtaa ctcttataga ccagactttt tccgccctgc
     3301 agttcgttgt tgactgcggt ctggaaatcc agtccggtat tctgtcgtac aaaagtccag
     3361 ttgggatgtt tctgtttcaa agcctgcaat gccggccggt agctctccgg gaactgctcg
     3421 atgtccgcat agacaccggt ggcattttcc tctgccagca taaccgccgc tccgggattc
     3481 atgccgtaca gttcttccca ttccagaaaa cgttcatccg agcatgccag ataatcccgg
     3541 gggatatatc cctcgtagac cttgcccttt acctcggcgg atactttttc ccagacctga
     3601 tagtcttcat ccatcgtcac atcccggatc tccaccatct gtccactggg cacggtgacc
     3661 acttttgtgc tgtcttccgc cgcttccgca cggatcgtca gatcatcgca cagatagacc
     3721 agtgccatga cctcccggtc ttctacgata ttctgcaaag cccctcgtgc ttcttcgatc
     3781 catgcctgat cttcttcatc caatgcatgc gtctgatagg tattgccctc gtaggtgaat
     3841 ataaatgccg ccaggagaat tgctcccaga aatgccaatt ttctcttcat ataacgtgac
     3901 tcattcctta ttttatggtt gctgtgttgc ccgttctatc ctgcctctac gtcgggcatc
     3961 actctatgtg cttcagtttc ttatgcagta cccggattcc cttcggtgca atggcaaaca
     4021 gtgtatgata acacttgttc tttttggtaa gccagggatt tttcatggct ctccaccagc
     4081 cgtgacgcaa gtcacgcacc accctgcggt agaatgcgtt gtccccggtc atctgggcga
     4141 tagggatgtg cagcagataa tccagccgct ggaagatccc gaaccggaat gcgatttccg
     4201 acagctccgg atatttttta taaaccagtg caagcaccat gtccgcattg tcgatattgt
     4261 cggcgaatac tctggagaac cggttcttgt ctctggtatt gctgtccgga cgataaaata
     4321 catgatacgc ctgtccggga atgcatgcaa tccccggaac cgcactttcc tcccgggtca
     4381 gcatttttac taataaatga aaatcttcat tcagtgcccc caccggaaat tgttctatgg
     4441 taaacaggtc cttccgcaaa agcttggtac agaaggagca gtctccgcgg tgcatcagaa
     4501 gttccttcag aaaaccttct gccgggatca cctgttccgt tgccaccggc tcgcagatat
     4561 tcggtaaaat attgccgtct ttgtcgattt catctctgcc gacctgcgct acgggcatcc
     4621 cggtcttttc gattgccccg tacagccgct catacatatc cgcatccacg tagtcatcgc
     4681 tgtccacaaa gcccacatat tctcccccgg cctgctgtaa tccgaaattc cgggcggaag
     4741 aaggcccgcc gttcttttta tggaagacgc ggattctctc atcctccgtt gccagccggt
     4801 cacataatgc cggtgtctcg tccgtggaac cgtcatcgat cagcaggatc tccagatttt
     4861 cgtaagtctg tgtccgcagg ctttccacgc agcggggcag atacggtatg atattgtaca
     4921 ccggcacgat cacgctgatc agcggttgct tggttacact actgttcact gtttattctc
     4981 ctatacatag accgctccgg gattacaggg gccggagaac aggggccgtc attccataat
     5041 tgtggaatca ccggggcagg tgttgtgatg tcctgcatca catcctggcc ggcatccggc
     5101 atttcctgtt cttctgacat ttctggcaag aacttcattt tccggcacag ttcgatcaat
     5161 ctttccgcag ccgtccgggc attccattcc ccgacaatgg tctgctgtgc gttttctccc
     5221 agtctgcggc actctccggg atccctgaga agccgttcca cggtccgaaa taattcctgt
     5281 tcctcaccgt cccggtacat cctgccgttt tctccctgac ggatcagcca gggagccgct
     5341 ccgatcatat gatctgccac cacagcacag ccgctgttca tggcttcatt caccactgcc
     5401 ccccagcctt ctttgcggtc actggtgacc agatagattt ctgatttttc catggccgcc
     5461 cggacttctt ccgggccttg aaaaccggga aaggtgatca cgtcggtcac tccgagttcc
     5521 tctgccaggc gatgcgtctc ttcctccagt tcccctccgc cgatcatggt aatatgaaac
     5581 gtattttctt ccagatgttc ttttaaataa gatgctgttt tcaccaccag ctccggatgc
     5641 ttccagtcga tcatccgggc cgcccacagg atactgcccg gttctttgcg atcgaagggc
     5701 tgccctgcct cgtaatgcct ggtctccggg aaatagccga agcgcagcat tttgccgggg
     5761 taggcatgga tcaggtgata atcgcagggc acataagctc ctgcgcacag gaaatacacc
     5821 ggtgccttac ggtatctggt gtgatccttg tatttttgca acaggcctct cggagaaaca
     5881 gccttccatt gcccttcctt atataagcgt tcattataac ggaagatcgg ttttccggca
     5941 gtcagtctct cctgaatata gctttcttca tcgcatccgc cgaagatcac cacatccgcg
     6001 gtaaacaata gtttctcaaa ctcttccgca tctgtgacgg cgttgctgtt cgactcagag
     6061 ccgggcgttt tctccacccc agacttatgt gcacacgaat tcttcgcagc gtagcgcacg
     6121 tagggacgtt cctcggcctg ccagcccatc ttcacccgct cttcttccat gggctgtgtc
     6181 tgcaggaaac agaaatctcc ctccagcaat tcatacagag catcacagaa gggcaattga
     6241 tgatgattaa aatagtttga gacaaatatc agcttcattc cttcctccct gctatgtttg
     6301 catagatttc ctgcagtttt gcgaaatttt gctctttatt atgggttttt cttgcatgtt
     6361 ctcttgcatt ttttgagtat tccagcattt tctccggaga actccacatt tcgataatag
     6421 aatttgccat ggaatttaca atattttcca attcttcttt ttttgtcgtt ttataatttc
     6481 gcatgttatt tttgcttcca gaagcatctg acgcatattt gtcattttca gccacttccg
     6541 acagctgatg tccccggcac cacagaccgt cccttccgcc atcaaacagg ctggggattc
     6601 cccctacctc cgtgctgatg cagggaactc ccagaagcat ggcctctccc agggaattcg
     6661 gtgaattttc cagagaggaa caacacagga acagatgact tttcagaaat ctttccttca
     6721 tttcttctcc ggtcagtttt ccgaggaagt gtaccctgtc ctccagctgt ccttcccgga
     6781 tcagtctgcg caggtaacgg ccatatgccg atattttcag cttttccttc ggcgtccggt
     6841 atgccgtcag atcatttcct gccacataca cctgtaccct gggatatttc tcacggatct
     6901 gtggcaaggc ctgcagcaga tagtgcagtc ctttcagagg ataatccgcc tgactgacga
     6961 aaatggaata gggttcacag tgctccggct gccattgtcc ttcataaaag gaagcccgca
     7021 gggtctcatt catcgtataa taatgtgcct tgggattcca cgcggtagtc actgtcctgt
     7081 cccaggcggt tcttccggtg atattgccgg caagtcctat tgcttccctc tccattacac
     7141 ctctgcgggc aaatttttcc tgctggctgc gcagggtatc cttcttcacc agatcccgga
     7201 aagtggtctt gtggatctcc gattccggca tatcggcaaa atatgcctcc gcacacaacg
     7261 tacagattcc ctgaattccc accagtatct tctccttgtg gggaaacacc cggcatactg
     7321 ccagtgtgtg tggatattcc gtgccgaaac agtggatcac atccggtgaa aagtcttccg
     7381 tgatccgatg cagttcttct tccagcaacg gctgatatct gtcggggtgt acggtgtctt
     7441 cgtggaatcc ataacatgtg atattatata tttcagcatc tgtatttgtc tgtaaatgct
     7501 cccggtcttc tttttcccgt ggcattgcta tctgcagtct ccagggcacc tccgtatctg
     7561 ccggcaccgg gaatgccaca gccagctcca ggtcactgga attctccgcg gctacctgct
     7621 ccaacagacc ggacagccag ccctctttat tcgaagcttc tttatgcaat gcttcggcta
     7681 tgaccggaag catgatattg cagacccaca gcactttcat aaattaaatc ctcttttaat
     7741 taaagatcca gtttagatac ggaagcagcc gtatctccac atcatacatt ccccgcaaca
     7801 acattacgaa atacaataga tatgctgcaa caacgccaat aatacacagg gttcggaacc
     7861 atcccttttt ggcatccttc agcacacccg gaatcaggaa aatctggctt tggatcaggt
     7921 aatatgccac ccgggatact tccgggataa aggagccgca ggaatagagc accagtcccg
     7981 ccagattcag gtagaagtaa taacggtttc tgcgattttc ctgaacgctg tccttccaat
     8041 agatcaggca cagtatcagc actgccaggc atttggcgat gttgacatag gataactgtc
     8101 cgttgtcgaa catggaatcc ttgtaatagg gatagaaccg gaagataata ttccggtaca
     8161 catcctgtcc gaagatcaga gataagatca gtaatacgcc aaccgcatag tgccactttt
     8221 tcagccggat ccggcacaag agatctatca caatatatgc cgggatgacc agcagtaccg
     8281 atttgtggat cgtacagccc agcaacaccc agagcagaaa cttcccatat tctccccgca
     8341 gcacatattt ctgcgcatac atgaccagtg ccagtgcgaa atagtaacgt accgaattca
     8401 tactattaaa gtaaaatccc tgtgtcaata acaggaacag ggaaaataca taccagtccg
     8461 cctgatcatg cagtgccttc aggaaaaaga agactgtaat cagggaaaag aagccaaaga
     8521 tcggaagata agatccctct ccgaaaatca gatggaaaaa tttcaccaga tacacaaaac
     8581 cggtttcgta ggagacatgc cttccctgat cgatcagctg aaatttcagc cagtagaccc
     8641 agtaatcacc gcccacagca atgcggcagg cagaaacccc ggccaggaga cagaagatgg
     8701 cgaattccgc cacagcattg cacgcctgct gtctgtccgg cccggccgcc atcagatccc
     8761 cggaggtatt cccacgaaag gagcgtctgc cggacagata ttctgtcgta tagtcacgat
     8821 tttttaccag aaaaccgaat gctacggtaa cagctgtcaa tatgatatac accagtgcgc
     8881 tcttctccat gtggaacctc ctttctttat ggcctttttt ccaaaaagca agctatcgtc
     8941 ctcctgcctc ccggatgccg tcacgcatcc actgtctggc ctgctgcagg cttacctctg
     9001 tgcacagcgt acccttatga gcatacaaaa accgcagtgc cagcggccat tccagccatc
     9061 catacagata atgatacagt actcctctgt catggaaaaa tttttcatta tagccatgaa
     9121 accaggtgga ttccccggct tcctctctgc cgatggtcac cggtgcggta tatacacggc
     9181 agcctttttt aataaactct gtcaaaaaca ggctgtcttc tccattgctg tattttgcac
     9241 cgccgccaaa caacagggaa aacgtgattc ccgatcgcag caggctctct ctgcgtacag
     9301 caaaactgac tgcgccatac cgcccgcaat tataccaacg taccggtttc cgttccgtaa
     9361 tatggtaggt gcggcgttct tcctccacct caatattgaa aatgatcata tcggcccggg
     9421 gattccgttc aaactccgca agaacagccg cctcataacc atcttcatac acaatatccg
     9481 catccgaaaa aaggcagatg tctccttccg cccgaaggat ggcctcattg cggcttctgc
     9541 cgatgccacg atcgggaaaa ctgcagaaac ggattcttcc gttccccgga gatatctgca
     9601 tttcctcctg ccccagccgg tcgcactgat tgatgatcac cgcatccgat tgcaggttca
     9661 tatgttgtac ggtctccgga agagaagcat tcattaccga tgccagaact tctaccttca
     9721 tgcctgctct cctccgtgtt ctgtattctg tgtctgcatg gctcccggca gaaaataata
     9781 gctgcggatc agctgttcat ccgcattcca caggaaggct ccgtccacag agcagtcctg
     9841 ggtatgcaga tactctaata ccgcttccag tctctttcct acccgttctc cggccggcac
     9901 cgccagcaga aatgcatctg ccttacgaag tgtctctcca ctctccgggc ataacaacgg
     9961 actgggaacc gctacatatt ccaccggtat tcccttccgg gtcttatctg tcttagccag
    10021 cttctgcaaa tcctccacga cttccttcgg atccgcttcc ggcagtgcca cacatactgc
    10081 cacacggcag atttccgcat tttcggactc ctttgcctgc tttttatcgt acactttctc
    10141 cagcgtatac atcagatttt ccgcaaatcc cacactgcgc accgttccca gactgttcag
    10201 tccgtagcgt ctgcgaagtg ttgccggcag ccagatgctg tcctgcgaga tctccaccag
    10261 caaaaagagg actaccgcaa aaaacagaga caataccgcc gccagaatga ctgcccgtac
    10321 cgggcgcaca tccggtacca cgacttctgc aaaatctccg tgatccagca ctttgatccg
    10381 gtcgatctcc acgatactct ctgcgaattc gtcacacatg gtatcttcta ctgcgtgggc
    10441 cagagcaata ctcatctccg gatcctgagt tgccgcctgc gtcaccggaa cactgaaatc
    10501 cgagggaagt gttgccgaga gatctgcgat ccactggtct ctgcccagag acagtgcttc
    10561 cgaagctcca tcgtctcccc gttccacagc ctcctgcaga tgcttctcca caccatccag
    10621 gaattctccc gtatggatca tggtgttcca tgtagcctcg ttaatgtagt acgcacccgc
    10681 cgcattgggg ttctctttat attctacctt ataagtggaa gaagccgcat accctggatc
    10741 gggctgcagc accacatttt ttacataata accgccaccg aacagtaatg ttcccactac
    10801 tgtcacagcc agtattttcc acagattgcg gcatagccgc agacacataa gtctcaggtc
    10861 gaagggctct tttccgtagc ttacttccat gcgtctacct cctgccttct cagtcgtcat
    10921 ccttctcggc acgaccatcg taggttgcct tgaaagccgt tacctgttcg ctctccacac
    10981 cctcggtact gtccggccag aacaatacct tcagtgtcag aagcatcaat ttaagatcca
    11041 gccacacaga ataattctcg atatatgtta aatccagttt taatttatca tacggagaag
    11101 tattgtactt tccgtatacc tgtgcgaaac cggccagtcc cgctttcacc ttcatacgga
    11161 atgcgaactc cggcatgact tccatgtact gtgcaataat ctccgggcgc tccggtctgg
    11221 ggccgatgaa agacatatct ccccgcagaa tattgatcag ctggggcagt tcatccagcc
    11281 ggcatttgcg gatcacctta cccacgggag tgatacgctt atcccctttc tgtgccagac
    11341 gtgccacgcc gtccttctcg gcatcagtgc gcatactgcg gaacttcata atatagaatt
    11401 ccctctgtcc ggtggtgcag cgcacctgct tgtacaatac aggtccgccg tcataaagct
    11461 tgatggcaag cgctgtcaga agcatcagcg gtgaagtcac caccaacagg atcaacgagc
    11521 agatcacatc gatagatcgc ttgatgaacc gctgctccat gctcagggag tactctctgg
    11581 tcagcaggat aggcgtgtca aatacatgca gttcctcgga gccaactaaa ataacatctg
    11641 atattttagg catgacatag acacggatgg aatgggcata gcagaatttc agaataatat
    11701 tacgatcctt ttcattaata tcccagatta ctaccgcatt gatctcaccg ttccggtaat
    11761 tgtccagaat ctcccggcac agttctgtcg tacctttttt aatgtgctca cattttgtga
    11821 tcttatattt gtccttacgg ctttcaaatt tgttgacaat gtattcgatg ggacgatcgc
    11881 cgtggatcag cagaagctct ctgggcggga atgctgtacg atagatcttg ttggcaatat
    11941 tggcatagat gatcaccgct atgatctgta aaaataccat caacaggaac atatccgccg
    12001 gaaaaatgct acgggccatc atacacagtt ccgcatagat cagcacatcc gccatcaggg
    12061 ttgcaaacac ctgggagaaa ataatctccg tgtttttcat gtatcccaga cgcatgccgc
    12121 cgtaggtcac agagaaaaat atcaggattg cactgtagat accgttttcc agcagaaagc
    12181 cacgatacca gatcttctgc aggggatcta ccagctggaa ctggaaatgg aactgccaca
    12241 tgtaggcaaa cagactgatc tccagcaaca gacagatcat cgacaaccct atattgatca
    12301 gacgcttaaa ggattccctt ttccgcagtc ggttttcata ataattaccg tttgtattca
    12361 aaacaaactc ctcctatact ctgcgtttta cggcgcgtac cgcccagaag cagaaatgcc
    12421 aggcactgtt cggcactgac atgccttccg ccttacgata cagattccag atcctccgca
    12481 aagcttccag cttattcgat gacagggatt tccctgctct ccgatatttt accaaatttt
    12541 ggtccaaacc acaagctaca taaccctccc gcagtattct ccaccacagg gcagtgtcct
    12601 cactcttgat ctggggcatc tgcagttgtt ctttcgacag ctgttccata tcgaacatca
    12661 cggtactggt aaagattgtg gtatttttca aagcttcttt ataagtaatc gtggccggca
    12721 cacggacaat ctttccgata cccttgccat tctcatcggc gaattcatag ccggtaaaca
    12781 caaaggctgc ctgtttctct ttcagaaaag ccagctcttt ttccagcttg tccggtgtcc
    12841 acaggtcatc tgcatccaga taggccagat accggccttt tgcttcgttg actcccagat
    12901 tacgtgctct cgccgccccc agattcgtag agtgagtcag cagacggatt ctcgtatctc
    12961 cggttctctg aatatattgt tctatgatct ccctgctgcg gtccgggccg caatcctcca
    13021 ccagcagcag ttcccagtgt ggatacgtct gtgcccggac gcagtccatc gtttcttcta
    13081 tataacgttc tgcccggtac accggcacca caatactgat cagttcctgt tccattttgc
    13141 tttcctgttc ctaattctta ctaatcgtta tagttttccc tatctttccg caagatgctt
    13201 acttacatca cgatcaggaa atatccgcca aaggaccgca gtctgccgcc cgcagcctct
    13261 tgcaattcct tctgtacatc ctccgacagg tcctccggca ataaaatgta gtctataccg
    13321 gccttcccgg cactgtcgag gcacgcagca aaatctattt tcctgccacc ggcttctgtc
    13381 gtggtatttt catctgtccg cagatattcc gtctccccgg tagtctccag gtggctcatc
    13441 cacagataca tatcttcctg ccaggtctca taggtatcat aggagtagct gcccagcgat
    13501 gcatcccaga tgcttctgcc gtataccggg tggatctcag cgctgtaggc tctggcagcc
    13561 tccatgacct ccttcggcgc ccacagactg tattcccgtg agtttccttt ttccgcattg
    13621 tatacagaaa gctgctccag aatttccctg gcgggagttc cctgttgctg tccgggcatt
    13681 cccgtcagtg ccggtatctg cgttgtctgt gccacggtcc attccgacac cgggtttcca
    13741 cccaggaaca ggatgcccag caggatcagc gcagccatcc cgtttcgcag gcttttctct
    13801 tttaataagt tacacagaaa gtccgttgct gcccatgcga tcaatgctgt ctggggtacc
    13861 gccgcccaga tccatacata agagtaaaag gcggtctggt attttaacag gatcagtgcc
    13921 gttacgggac agatacagcc aaatgtcacc agcgctccgt aaagatacag ccgtttccgt
    13981 gctttttccg ccccggatgc attggcttcc tttccggaac catagaggac aaatcccaga
    14041 tacaggatcg ccagcagcaa aaatgccgca aattttccat gttgtgtata gtcggtccag
    14101 cctgtcaggg cggactgcag aagtgtcatc attctgtagc ctcctctcct gacctgccat
    14161 gtgcatcagt cattttccgt ttttcccatc gtttccggaa ctgggaaacc aacgctccaa
    14221 gtatcagcca tgccacagta acaagcaggc agactcccgc gccatgcagg gtccagacta
    14281 tgcaggcctc cgccaggata caaaggatca cgcccgtata ttttctgtcc atcagacagg
    14341 acagcagata cggaagcaac accgccgccc ggatcgtcac accgcggaat cctccgtaaa
    14401 acacatcaaa tccatccact ccgggcatat aggcccctgt actgaacagt attcccacga
    14461 tcagcaaaaa cgtccgtctt ttcaaagaat tttccggaaa aagtgctttc cccaggcttc
    14521 cgtaagctac ataagacaac agcagcgtaa tgcaggggat cagacgatac accacgtcta
    14581 cgggtgccat acccgtaaaa cggctgatgg ccccgtacag agtgggcagg cacaatattt
    14641 tcagccgtaa ggacatcccc atcgaatatg ccgtccccgt gagaggattc acggtataaa
    14701 cagcattttc cctgagaaat gtgttcacgg tctccaccgt catatctccg gtcagccaca
    14761 tgcggttccc cgtcagtatc cgcaggatct gcagcaggat cagaaatatc aggacgcccg
    14821 tgagaatctg tgtgcttttt tcctttgccg tgtctgtccc gctgttttga tccgcaaccg
    14881 gatcattttt accggctgtg gcccgtttcc gtgtctgtct ggatattaca gacaataata
    14941 ttgccgccag cagaacgatt cccacctctg ccagcagcag atccgttaca gtcaaaaaag
    15001 accaccctaa caggcagcct gccaaatggg cgatctctgt cagcccgata atgatcaggc
    15061 tccccccgag caaaatatcc gagggccgaa aagtcatttc ttctcccaga cccgcctgtt
    15121 tcgtacccgg tcttttgtac atcttaccat atttatatgg acttgacaaa tataacagca
    15181 tttccctgca acaaaaaacc gcgtaccgga aacttcccca ttccgatacg cggtctttct
    15241 cagtctgcag aggagaatac tgcatctgct taattaacaa actctccctg ttccaaataa
    15301 ttaaaattaa tatgttacat agccttataa tccagtcgga tgttgtcggc gatacgtgcg
    15361 atgtactcgc tgttcgtagg tcttgtcctt ccggaggcta cagaatagcc aaaaatcttt
    15421 tcgaatgtct ccacatttcc tctggaccag gacacttcaa tggcgttcct gatcgcgcgt
    15481 tccactttct gatctgtggt cttgaaatgt ttcgcgatgg tgggatataa cagcttggta
    15541 acgctgctta ctacctccat gtctttccct gacaaaatga ttgcatcacg caaatagtga
    15601 taacccttca aatgcgccgg cacccctatc tccaacatga ttttggttac atatctctct
    15661 aactctgaat cttttactgc aacaatatcc aaaacaagtc ccccttttta cttgtcgagt
    15721 attgctcctc tgcagtgact gatagagaaa tcatatcata gctgtcgata tttgaaaaga
    15781 catttttccg ttaaaatata gttaagaaac taaaaatgtt acattttacc cctattttgt
    15841 aacattttta cgtacataaa aaacggatat ttatgcggtt tttcttaaat ttcctctttt
    15901 accaggtaaa tcggacggcg ttttacttcc atgtatgcct ttgccagata ctgccccaga
    15961 atacccgtac agaaaaactg tacgccgctg gtcatcagga tgatgcacac cagagaaggc
    16021 cagccggaca ccggatctcc gaagatcagc ttccgcacga tgataaatat gatcatcaaa
    16081 aaagcgatga tacagaacag cacacccaca aaggaagcga tcagaagcgg tgccgtggaa
    16141 aatgcggtga taccgtcaag ggaatatacc agaagcttcc agaaattcca cttggtctcg
    16201 cccttggcac gctctacatt ctcatattcc agccatttgg tcttaaaacc gacccagccg
    16261 aagattccct tggtaaaacg attatattcc ctcatggaca gaatagcatc caccatctgt
    16321 ctggtcatca ggcggtaatc tctcgctccg tccatcattt ccgtctggga aatcttcttc
    16381 atcagatggt aaaatcttct ggcaaaaaag ctgcggatcg gcggttcgcc cttgcgggtc
    16441 acgcggcggg ttgctacaga atcaaacccc tgccagatcc attcaaacat ctccggcagc
    16501 aaggaaggcg gatcctgcag atccacatcc atcataacca cgtaatcgcc cttcgacttt
    16561 tccagtccgg caaacatcgc cgcttccttt ccgaaatttc tggagaaaga gacaatatgc
    16621 acacgggaat cctgttcgat cagctttttc acttctctca cagtgccgtc tttggaaccg
    16681 tcatcaatat agagaagctc cagttctatt tccttttcct tgaaaaaggg atcatttttc
    16741 gtcaattcct gataaaacag agcaacgctt tcttcttcat tatagcaggg aacgatcacg
    16801 ctcaataagc tcattttgta atctccttat gttttcctgt cgctttttca ggcttatctg
    16861 cgccactgtg cgacaggtaa caactatatt atactatcct tcgatgcatc atttcaaggt
    16921 caacactgta ctttttccgc tttttggatt atactggatt tatatatgtc tgttctgcaa
    16981 gatacagcat ataggaaacg aggttattta tgactttttt tgaacagttt ttcagcaata
    17041 ccattttcct gactgcagcc ttcggctggc tggtggcaca ggttctgaag acgctgatcc
    17101 atttgatcgt ctccaagaaa ttcgttgcgg agcgtctggt cggcagcggc ggtatgccca
    17161 gttctcactc tgctaccgta tgtgccctgg taacagcggt ctgctacgaa tatggtgcgg
    17221 gaagctttga atttgccatt tccctgatcc tggcgatcat tgtaatgtat gatgctatgg
    17281 gagtacgcag agagaccggt attcaggcac aggtattgaa cgaaatgctg aaggtcttcg
    17341 aagatatggg ccgcagtgag atttccgccc atgataaatt aaaagagttc gtgggacata
    17401 ctcccctgca ggtgttgatg ggtgcgctgt taggcatttt atgcggcgtg gtcattgaga
    17461 gcgtctaact ctgatagccg atcgaggggt gattgcatgt gtaaatttac ttgtcgacgg
    17521 cagtttctgc agactgcggt gcctcttctg ctgactgtct ccataatgct taccggatgt
    17581 ggacagacgg gtaactccaa ttccccggtc cgtccgatcc tcccggataa cagcccggat
    17641 tcctccacgg tttcttccgg ggaaaagctt ccggatcccg taacgatcgt tgtagaggat
    17701 gattccactc ctcccgctga gggcatggtc cgcagccgtc ttacgaacga atgggtggat
    17761 gccgatgtgg ctgctacacg ccccattgcc gtaatgattc ctaatgaggc atctgcaatt
    17821 ccacaataca gtctgtccga tgcttctatt atttatgagg cgaatgttga aaaccgtatg
    17881 acccggatga tggcaatcta cgaagactgg cagaatctgg acaaaatcgg caatatacgc
    17941 agtcttcgtg attattatgc ttactgggca ttcgaatggg atgcttttat cgtacatttc
    18001 ggcggtccgt ttttcatcaa tgagttgctg gcgcagccgg atactcagga tgtggacggt
    18061 acgtccggca gtgatgaggc tgcctttttc cgtactaccg accgcaacaa gccccacaat
    18121 gcttatgctt ccggtgaagg gctgcagaag gtgatcgaga aaaagggcta cagcctggga
    18181 taccgcggac tcagcgatga gaatcatttc aggtttgcca ccaaggcgga accgaacacc
    18241 cttgggcagt atggtgccaa agcaaaggat gccacctata tcgatatgtc cgggtgttat
    18301 ccgctgacaa ggtgttattt tgaatttaac gaggatgacg gcctctatta ccgttcccag
    18361 catctgtccg gcagcacaga cggccctcat atagatgccg ccaccggaga gcagctgact
    18421 ttcaaaaata ttctggtaca gaatacgttt cacgaggaac tgggcgaggg gtatctggct
    18481 tttcagtgtc atgacaccac ccgggacggc tggtttttca ccaatggcag aggaatacat
    18541 gtgaattggg agaagacttc cgattacagt gccaccagat attacgatga caacggggac
    18601 gagatcatcc tcaataccgg caagaccatg atctgtatta tcgaagatgg agattccttt
    18661 acgttccatt aatgtaaatg gaaagttagt aaagaaatgc tatcaaatcc ccgtagccct
    18721 tggcaaggt
//
LOCUS       DAWDHD010000034        18618 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_203_length_18618_cov_13.322900, whole genome shotgun sequence.
ACCESSION   DAWDHD010000034 DAWDHD010000000
VERSION     DAWDHD010000034.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 18618)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 18618)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..18618
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_203_length_18618_cov_13.322900"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5415..5424
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5056 a         3876 c         5117 g         4559 t
ORIGIN      
        1 cgaaacggaa cacgctagaa gcagaaactt agacacagag aaactatcca tgaaaatatt
       61 caacggagaa atcattaacc tagtataatc agaattataa agtagaaatt ccccagttcc
      121 cggaccggga ggtgtccata gcagaatacg gcgcgaagag cggcgatttg cagatcgaga
      181 ccggcagaca caatgcggag gccattaacc gcgcgatctg cgaagtgtcg gagcaggggg
      241 gcggtacggt aagagttccc gcaggcatct gggcggtggc tccgctccgg ctgttaagcg
      301 gtgtcaatct tcatctggac agtcaggcta tgttaaaatt catcaagtcc aaagaggact
      361 atccgctgcg gatcaccagc tatgaaggtc aggactgcat tcgcaccgta tctcccgtta
      421 ccgcggaaaa tgcagaaaac atcgccatta ccggagaagg catcattgac ggcagcggtg
      481 ataaatggcg tccggtcaag aagtttaaga tgacggaaaa gcagtgggat gcgttgttta
      541 ccatcagtcc ttatgtgtta gagacgaaag aaacagcgat ctggatgccc acagagtcga
      601 ttttaaaagg aaatcaccac aatattcagg gaaacaccga ggaagcactg gccgccgcgg
      661 caccctatta cgatttttac cgcccggtga tgatcagtct gcgccattgc aaaaatgtgc
      721 ttctgcaagg cgtaacgttc atgaactctc cggcatggaa tatccacccc tatttctgcg
      781 agaaccttac ggtggagcat atacaggtcc gcaatcctta ttatgcccag aacggtgacg
      841 gcattgatgt ggagtcctgc actaatgtgc atattcatca cagtgttttt gagaccggag
      901 atgatgccat ctgcatgaag tccggcaaaa atgccattgc ccgcaagatt caggggccct
      961 gcagcaatgt ttacatccat gactgtctgg tgaacgaagg ccatggcggt ttcgtcatcg
     1021 gcagtgagat gtcccgcgga gtaaaggata ttctggtgga gaactgtact ttcgtgggaa
     1081 ccgatgtggg tgtccgcatg aagagtgcgc tgggccgcgg cggcgtggta gaaaatatca
     1141 ctctccgcaa tatccatatg agcgagatca agggcgaggc agtgatcctg accatgtcct
     1201 atgtgctcaa ttccctgaac cgggaagaga ccattgccat ggaaaatgag gacgacatcc
     1261 cgtatttcag aaatctgcag atggagaata ttgaagtcac cggctgcaga cagttcacaa
     1321 agatcgagcc tttacagggc aggccggaga caattcaaaa tgtagtcatt aacggacaga
     1381 agctggcata agtgccagga ctggaacaca gatttccggt gccggagaag aaaattttga
     1441 aatacagtat caggaggtaa tgattatgcc atgtacaacc atattagtag gaaaaaaggc
     1501 atcttatgac ggttccacca tgatcgcccg gaatgacgat tctccttcgg ggcagttcac
     1561 accgaagaaa ttcgttgtgg tgcatccgga agaacagccg aaaaaataca aatccgtgct
     1621 ttcccatgtg gagatcgatc tgccggagga tcccatgcgt tataccgcag tccccaatgc
     1681 actggacgga gagggtatct gggcagcgca cggtatcaat gcggcaggtg tgggcatgac
     1741 ggccacagag accatcactt ccaatgccag agttctggga gcggatccac tggtggaata
     1801 tgttccggcg aaggatggtg tggaagagat ctccggcggc atcggcgagg aggacattgt
     1861 gtctctggtg cttccttaca ttcacagtgc cagagagggt gtcacacgtc tgggaagcct
     1921 gttggagaaa tacggtacct acgagatgaa cggtattgct ttttccgata aaaacgagat
     1981 ctggtggctg gagaccatcg gcggccatca ctggatcgcc cggagagtgc cggacgacgc
     2041 ttatgtggtg atgccgaacc agttcggaat cgatgccttt gatctggaag atgcgctgac
     2101 ggagcaaaag gagcatatgt gttccgcgga tctgaaagaa tttattgaaa agaaccacct
     2161 ggatctgtca caggacggca gtttttccgc cagagatgcc tttggaagcc atgacgattc
     2221 cgaccatgta tacaataccg caagagcctg gtatatgctg cggaccctga atccccgtac
     2281 aaaggtctgg gagggcccga atgcagagta cacaccgttt tccgatgatc tgccctggtg
     2341 catggtgccg gagaagaaga tcacggtgga agatgtgaaa tatgtgctgt cttcccatta
     2401 tcagggaacg ccctacgatc cttatgcggc ttatggagat aagtccatgc ggggagccta
     2461 tcgttccatc ggcatcaacc gcaatgacgt catgaccctg atccagatgc gtccggaggg
     2521 agagccggtt cagtggctgg cgctggcttc taatgccttt aatgtactgg caccttttta
     2581 taccggcatc gataggacac ctgagtacct gtcagcgacc accggaaaag tctccacgga
     2641 gaacttctac tggatgagcc gtatgatcgc agccatggca gatgcttctt acaacaaatc
     2701 cgtgttccac attgagcgct atcaggaaaa tgtggcggcg aagagccatg tgattctgaa
     2761 tcgctacgat gcattgctgg cgcaggagaa cgatgaggac aaatgtaaga aattgcagga
     2821 agaagccaac gaaaagattg ctgggatgct tcagtgcgcg gcagccgata ctttggacaa
     2881 ggtcctgtat gaactcagtg ggcagatgaa aaatgcctat gccagatcgg atgcgtaaac
     2941 aatcatccag gtggaaactt tatgatctga atgaagagaa aggaaaacag cagattctcc
     3001 aaaagagtct gctgtttttg tgtttatgaa aatatctcat tgacaaatct gaaatgttta
     3061 catataatta ccaaagataa ttactagtgg taactataaa gacaattaaa gaatagaaaa
     3121 aagatatcag agaggaaaaa gaatgacggt tcagaccata aaacgaatgt tggatgcctg
     3181 ttatcaggca aaaagaatcc gggagatgct tcccgcgctg ccctcaggcg tgacaccctc
     3241 ttatattcat tttctggatg tgatccagat catggagagg cagggggttg ctgtgaagat
     3301 ttcagatatc agtgactatc tgaatatccc ccgtcccggc gtgacacgga cagtaaaaga
     3361 aatggaagaa aaggggtatc tgcggaaagc caatgcggcg gaagacggta ggatcaccta
     3421 tatttccctg accgaagagg gacgaaagct gtcggaaaaa tataacgaga attatttttc
     3481 cggtctggca ccgtatctgg agagcatttc cgaagcagat gcgaaagtga tgatacagac
     3541 catagagacc ttttatcagg tgatgtgcga gaggagagag tattttgaaa aataaagaaa
     3601 atgacatgat ggattttaca cagggaagta ttttgaaaaa gttgcttcgc tttatggttc
     3661 ctattttggg agctctgatc ttacaggcgg cctacggtgc ggtagacctg ctggtagtgg
     3721 gacggttcgg ctccacttcg ggattgtccg gagtctctac cggtagtcag gtgctgaatc
     3781 tggtaacttt cgtggtgact cagctggcaa tgggtattac tgtgctgatc gcacggtatc
     3841 tgggagagaa gaaggaggag cagatcggca gtatcatcgg cggcggaacc gttgtatttg
     3901 cagcgatctc gctggtgctg tttgtaatta tggtgggctt tgcccgtcct attgcggttc
     3961 tcatgcaggc accggaagag gcggtagggc tgaccgctgc ttacatccgg atctgcggtg
     4021 ccggaatctt ttttattgtg gcatataatg tattgtccgc gatctttcga ggacttggtg
     4081 acagcaattc accgctgctg tttgtcttcg tggcatgcat tgtcaatgtc gtgggtgatc
     4141 tggtgctggt ggcaggactg ggcatggatg ccatgggagc agcgctggct actgtttttg
     4201 cacaggcatt gagcgtagtg tttgcaattg tacgccttgt gaaaaaagga ctgcccttta
     4261 ccattaccgg atacgatttc cgttggaatc cccagtgcgg taaattcctg cagattggtc
     4321 tgccgttagc tttgcaggag tgtctgacgc aaggctcttt cttggcactg tgtgcctttg
     4381 tcaaccgcct gggactggaa gcttcctctg gctacggcgt ggcatgcaaa atcgttaact
     4441 ttgccatgct gatccccggt gcactgatgc agtccacttc ttctttcgtg tcacagaatg
     4501 taggtgccgg agaacagaag cgggcgaaga agaccatgtt caccggtatc ggcatcggac
     4561 ttttggtggg atgctttgcc ttttcactgg tgttcttccg gggggatctg ctggcaggta
     4621 tcttctccgc ggatgcggca gtggtgcaga gagggtatga gtatctgaaa ggtttcgcac
     4681 cggagacact attgactgct gtgctgttca gcatgatggg atattttagc ggtaatggca
     4741 agaccgtgtg ggtcatgctt cagggactga tccagacact tctcgtccga cttcctttcg
     4801 cttactatat gagtatccaa ccggacgcaa gtctcaccaa tatcggcctc gcagccccca
     4861 tctctactgc agtgggcatt gttctgaatg tggctttttt tctctatttg gaacatgctg
     4921 ccaagcgtag ttaattgaga gttacgcggg caatgagccg cgcataggca tagtgaggac
     4981 agcgatgggc gcttgcgcac aaatcgctgt cttattcgat acgcggggcg taatcatcaa
     5041 atttagtcaa ttaagccccg gcagtcttac gatagcgggg cttaattatc aaatattgtg
     5101 cgtaacgccc cataccttgg tttaaggata cagagaagct tgttcctatc acctgtgtcc
     5161 acagtgaggc ggaaggtttc cagcagaaaa ggactgctcc ccgggcgtag atgcggcgga
     5221 gactgtgaga ttttcttaga aagtggttaa gccgcagggg caacgcgaac acgatgttcg
     5281 ctttagccgg taagagacat ggatggcagc gtaaaggcgg ccccgtcatg atgagaaaca
     5341 cttgaccact ttcctttaga aaatccacag ttgtagctag catccgagtc cggggcgagt
     5401 cgtttctgcg tgggnnnnnn nnnnccaccc ccgcgatctc ccctgtggcc ctcgtcggca
     5461 gccgcacctg ggtctaagcc ccagagtcgg gggggagtcc tttctgcgtg ggggctatgg
     5521 caaccctacg ttacgagcca tgtccccaat tcataaatgt ttcgcaaagg gcactccaca
     5581 tttgttggca gttctctgca atgagtcacg ctattcataa gattgtcagc aaaaacgctg
     5641 caaatcctat gatcaatttt ctgttttcat tcgtcgtttc gcctcataca ttttggcatc
     5701 ggcctcccgg accatatcgt ccatggaacg aggcatatta tgggtcagta cggaatatcc
     5761 catggctaca gtaatgggat gctcggaacc gtcaggaagc ttcagagttc ccagagacgc
     5821 ttcgaaatct ctttgcaact tgagaaattc tttttcggaa tcggatacgg tcgacaggaa
     5881 tacgaactca tctccgccga tccggtaaca ttttcctaag gaaccgagat gcgccaacag
     5941 agtatccgct acccggcgga tcatttcatc accgctttgg tgaccgtagg tgtcgttcac
     6001 atatttgagg ctattcacat cgtacagtgc cagcaacaca tgaaaattat gggatgcgtc
     6061 caatcttttc aattcttcca tataggaagt acggttcagc aggtcggtca gaccgtcgcg
     6121 ataagccagg cggctgagca gctgcatgcg aacaccttcc tgatagttgg tcatgaggcg
     6181 cagggtaacc tggactgctt ccatgatcag gaacataagc acaccgatcc gtgtgaagaa
     6241 gctggtctcg ggagcactgc cgaaccatag cagcaggata tccagtagaa cgccaaagca
     6301 cagacacaga attccaaggt tatgatatag attgtgcttc agatgctgcc tttgattctg
     6361 ataaaaccct ttgccgatgg catagataac aagtgcgatc agaatgccaa aagtaatatg
     6421 ggtcagacga agactttcat ggaagtcctt gagaccggtc agattcaggc agaaacgtat
     6481 actgaaatcc atgacactga ttaggcatac tgtctgtacc atcacagggg agtactctgt
     6541 atacattttg ccaaggtaca gaaacagtgg aatggtgatc agcatcataa aaagttccgc
     6601 aattgtatga taaatatggc tgttacgcag gatcagttgc agcatacggt tatctgccaa
     6661 cataaagata ccggtattca gagcaaataa tcccagataa agcatctcac tgccgatgat
     6721 gtgcttcttg tgtaatggca gataaaacag gataaatgcc agaccaagat gggcgatcag
     6781 gaagctgatg ccgaatccgg gtgccttgtc gcggatgcgg cttatcagga tatcttcact
     6841 acgtcccagg tacatatgat tgatcttgcc actgccgtct ccgtaaggat tatcgatcac
     6901 cagggtgagc gtgcgcccgg aatcttcccg gtagatgggc agagagacat aagaggctcc
     6961 cggcgtcttg ccgaagaaaa ctccctccgg tcgtatggcc tcatagatct gccggtcgtc
     7021 tatcaggatc gtgacattgg aacttttata aaaaaagaaa agtgccatgt cgtgagtcag
     7081 cgcaggaagc ttcttggtca ctgtgcaggt gccgctgata tgggaaaaat caacggggct
     7141 tccatcggag gtggtccagc cttctgaaaa gtcaatataa tccgatattg ccggctcacg
     7201 gttctgcctg ggcagactgt agtacagtac cgtaaaaatc acagaacaca tgatcaggaa
     7261 aaacagtcca cgtgtaatgg aatatgaagt tttgtgattg ttcgtgttag tattcatatg
     7321 aattatatta tagtaattag gtaccataaa agtcaatgcc tgtgttggtc agatcaatga
     7381 ctaaagataa aaaaaagttg caatgggtga aaaagtatgc tattgtgatt ttgttgccgc
     7441 tttagcgcgg taaagattta ctctggagag tccttccacg gaaagcaatt atcgtatttc
     7501 cgtatagaag gcgccgaagg tgtacggcag taaagctgcc aatctctcag gcaaaaggac
     7561 agagcatcta tatgcttatt cccattctct tcggaagaaa tcaatctacg ataggacgtc
     7621 acaagaggtg acggaaggag agggaatatg ttagacatga ttagtaactt tctgaccaca
     7681 gtggatgatt tcgtctgggg catccccctg atcgtcctca ttctggcgac cggtcttttt
     7741 cttacagcca gacttcattt tttgcagatc acgaagcttc cacatgcaat caaacactta
     7801 attgccaatg aaaagtcggg cgaagacggc gaagtatcca gctttgcggc actgtgtacg
     7861 gcattgtccg ctaccatcgg aaccggtaat atcgttggtg tggcaacggc tattgtagca
     7921 ggcggccccg gagctttgtt ctggatgtgg atcgctgcgc tggtaggtac ggctacgaag
     7981 tattccgagt gcctgctggc ggtgaaatat cgtgtggtag cagaggatgg acacattatc
     8041 ggcggcccct tctactatat cgaaaacggt atgggcaaga gctggaaatg gctggcgaag
     8101 atcttcgctt ttatgggtgt atgcgtagga ttgttcggta tcggaacctt tacccagatc
     8161 aacggtatca ccagtgcggt acataatttc ttcgatgcaa ataatgcaca taccgtacag
     8221 ctcttcggta tggactattc ctggagcgtg gtgatctccg gaatcctttt gaccatctgc
     8281 gtggcactgg ttgttatcgg gggattaaag cgtatctcca ccgtggcaca ggtcatcgtt
     8341 ccttttatgg cagtgactta tgtagtagcc tgtctgttag tattgatctt taatgtaact
     8401 gccattccgg cagctatcgt aaccattgta cagagtgcgt ttggtatgcg tgccgtagcc
     8461 ggcggtgctt tgggagcggt tatcatggca atgcagaagg gtattgcgag aggtattttc
     8521 tccaacgaag ccggtatcgg tagtgcaccg atcgcagcgg ctgcagcgca gaacgactct
     8581 cctgccaaac agggtctgat ctcaatgatg ggtaccatca ttgatactct gatcatttgt
     8641 accatgaccg gtcttacgat cgtaattacc gattcctgga acgtaggtct ggacggcgtg
     8701 gatgttacca ccagagcttt ccagcagggg ctgcctttta acagctttat tgcatccttt
     8761 atcctgatgg tatgcctggt attctttgca tttaccacga tcctcggttg ggattactac
     8821 agcgagagat gcctggaata cctgatcggt cagaagccga aggcaatcaa agtataccgt
     8881 tggatctaca ttgcctgcgt attcatcgga ccctacatga ctgttaaggc agtatggacc
     8941 atcgcagata tcttcaacgg tctgatggcg atcccgaacc tgatcgcact gttggcatta
     9001 aatggtgttg tggtggctga gaccaagaaa taccttgaca atgccaagaa aaagttttaa
     9061 aaaagttctg tagccctatc acgcgggaaa atctatattt ttcccggaag tgtagaagtg
     9121 aggaactata tgaaagatct tacaaagggc agaccgtcag ttttgatact gacgtttgcc
     9181 cttcctattt ttttggccaa tttattgcaa cttacttaca gcctggcaga tacccgcatc
     9241 gtaggtactt ttctgggaga taatgccttg gcggcagtgg gggccacgac gacgctgagc
     9301 aatctgatcg tacaattcct gatgggaatg tgcaatggct ttgctattat ttcggcacag
     9361 tgcttcggag cgaaggatat ggacagattg cgcagatcct tgggcaattc tctggtgctt
     9421 ggcctattgg ccgctgtggt actgacgata ggagggcttg tatttttgca gccgatcctc
     9481 agatttctga atgtgccgga aaatctgctg aacacagcaa cacagtatgt atccgtgatc
     9541 atagcgggac ttgttgttac attactatat gatgtattag cggcgaccct aagagcgatc
     9601 ggagataccg tgaccccgct gatcgtgctg gcggtgtcgg tggtgctgaa tatcggagga
     9661 gatctgcttc tgatcgtggt gctgcatatg ggagtgctgg gtgccgcagt tgctactgtg
     9721 ctgtcgcagc tgatcgctgt ggtggtgtgt gcggtctatc tgtgggaaaa gtatgaaatt
     9781 ctgcggatac gtgtggatga tctgaagctg caggatgccg gaatgctccg caatatgtta
     9841 agctccggtc tttccatggg attcatgagc tcgctggtca atatcggtac attgacattg
     9901 cagacctcca tcaataagct gggacagaat attatcattg cccatacggc agcccgcaag
     9961 atctccgaga tcttcatgat catgttcagc gtcttcggtc agacaatggc gacctattgc
    10021 ggacaaaatc tgggagcggg caaaatcgaa cggatccgca gaggaatcct gttggcaacc
    10081 ggttatacct gcatctggtg tacgcttaat attgtgactg cttataccat cggtgactgg
    10141 ctggtatggc tagtcaccgg cagtgaaaac ccggaagtgg tctacaatgc aacgttatat
    10201 ctcaaggtag atactttgtt ctattatgtg acggcggtaa tctgtattgt gagaaatgcc
    10261 atgcaggggt tgggagaacg tatcgtaccg ctgatctcca gctctctgga aatgataggg
    10321 aaaattgtga tcgcggcgac gctggttccc atgttcggat acctgggtgt catcgcggcg
    10381 gagcctattg tgtggtttat catgatcatt ccgctattga taaagatact gcgtatgccg
    10441 ttgtggaagc agaaaactta actgtttcgt aaacaaaaga gatattttca cttaacgtta
    10501 aggaaaaatg accaaattta acaataaaat acttgtaatt ttgtcgaatt gtattactat
    10561 atggaaaatg ttacaatgaa tttaccgaga aaatacttaa acgttttcga gatggaccga
    10621 atgtgataaa gtcttttaca gaaaggcaga tgtggtaagt atgaagaaga cagagaatag
    10681 gaaaacgaaa gtaaaaggga acaaaaagcc atttttcaca atttcgaaaa agatactggc
    10741 gttgagcctt ctgccgatga tcattgtatg tgcactagtg gctacggtgg gggccaaagc
    10801 attagagacc ggaattgaga aacagattga gcagtctctg cagatcgttg gtgtcagtgt
    10861 ggatgaaacc tacacgaatc tgtatgaagg cgattacacc agagataagg gaggcaaggt
    10921 gcgcaagggc ggcacatcca tttccgggga gacacagttg atcgatggat tgcaggagaa
    10981 gacaggcttc caggtgtctt ttctgtatgg aaatatgcgt ctgatcacga cattgaccaa
    11041 gccggaagga ggacgtatta acggtacggc gctggaagac gatgtctatg cacagattca
    11101 gactggggag gctttgttcc tgacggactg caacatttcc gaggtagatt attatgtata
    11161 ttatcagcca ttgatcaatt ctgatggctc tgtgatcggt gccatcgaag tggcgacgcc
    11221 gatgcagaat gtcaaggata caatctcgat gcaggtgaag gatattattc tgattgctgt
    11281 ggcatgtgtt cttgtggcgg caacgctggt aagcgtatta tcccgtagca tggtgaagac
    11341 catgaagaag acgaagcatt tcctaggcag gatcgtgaac ggagaactgg atgcagaacc
    11401 ggatgaaaag cagtgccgta aaaaggatga acttggagat gtatatcgta tttctgtaaa
    11461 actgcaaaaa actctccgtt cgatcgtaac agagatcaag gattccgcgg atgatctgac
    11521 agcatcttcc aacaaactga tcaagatggc acaggatacc caggaatctg tgaatgatgt
    11581 ataccatgct gtcggcgaga tatcagacgg cgcaaggaat caggccaatg agacaacgga
    11641 tgctaatgaa aatgtagtgc gtatcggcga gcagatcgaa tatatatctg aggaagtaaa
    11701 atctctgacg gattatgccg gcaggatggc agaagcagag aaagcctccg aagagatcat
    11761 gaaggaactg aacggctcca acgagagcac caaggaatct gtcctgcggg tagcggaaca
    11821 gattacggat atgaacggag caattcagaa tatcaccaag gcagtatcca tgattcagga
    11881 tattgcggac gagacagatc tgctgtcact gaatgccagc attgaagcag ccagagccgg
    11941 agaagcggga agaggttttg cagtggtagc agagcagatc agcaagctgg ctgaccagtc
    12001 taagcgctcc gcaaccgaga tcgagcagat catccaaaat atcatggagt cttccggcag
    12061 aatggtggag atcatgggtg aagtggaagc caacatgaat caccagcaga gcaagttaga
    12121 ggagaccggt gtgaagactg cggcagtagc ggatggtgtg gagaattccc tgcagcatat
    12181 cggcggtatc cgggagaaga tggatgtctt aggacagtcc ggaagcgcga tccgtgatgt
    12241 agtggaagga ttgtcggcaa tctccgaaca gaatgcagca tccgcagaca gtaccatgca
    12301 ggcggctcat ggcatgagtg ataccatgac agaactgatg aattcttctg agaacctgtt
    12361 ggcattagcg gataagctgg aaaaggtact ggatgttttc aaggtataaa gagggggccg
    12421 gaagttgtcc ggcagggaga taacatataa atgacagaaa atgcagtcca gttttcacag
    12481 aaaaccggac tgcattttcg tgtggtttat tgacggagat accatcatga attacaatat
    12541 aaggagtgta aggaattaca ttatgaatat atcatacggg agagaaggag aatgcaaatg
    12601 aaagagttgg aacgaagtaa ccgggtggcc atgattgcac atggtgtgat caatgcatgt
    12661 atgcttttca ttagcgtgat cggatttgtt gaacacacag tgtcggttcc ggtattggta
    12721 gtgttactgc ttcttgggat cataccggtt cttgcagaat ttatttgttg gaagagggat
    12781 catgcaacca aagctataaa acatctgtca ctgatcggat ttgcgttgtt ttatacggtg
    12841 ctgcttttta cggcacagtg caacatggtg tatgcatttg taattcccat gatgtttgca
    12901 gtcatgcctt atcatgatgt caaggcattt gcacttatta atgtggggac ggtggtagag
    12961 aatatcctgg tggtgctcct gggagccaca caagggggat ttggctatct ggggcaggac
    13021 gcaggattta tacagatttc ggtaatgatt ctgctgtgca ttacatccat ctatgctacc
    13081 atatccaacc agaagaacac cgatgaaaat atagagagca ttacagctgc gcaggaccgc
    13141 gcggaagcaa ccttacgtga ggtcatggaa atgagcagcc ggatggagac ttcagtatct
    13201 gatatcacag cggaattgaa taaactggaa actgcttttg acagtaccaa aacagccatg
    13261 gaggaggtgt ctgcaggttc cggggagtct gcagcggcga ttcagcagca gacgtcccag
    13321 acggaggcta ttcaggaaaa agtaaatact gtgggtgaag tggcagagac gatcggaagc
    13381 aatatggaac atacgataga aatgctggat gccggaaaag aagagatgac aggtctgaag
    13441 gagcaggcag aggattctgc aagaaacagt gaactggcgg cacagaagct ggaaacgctg
    13501 gatcattata tgcaggagat gcattccatc gtggaaatca tcagcaaaat agctaatcag
    13561 accagtcttc tggcgctgaa tgccagcata gaagcagcca gagccggtga agccggccgc
    13621 ggtttctccg tagtggcgac ggagatttcg ggaatggcca cccagacaaa ggatgctacc
    13681 ggtaacatcg cggagttgat ccaaaatgta tccggagcga ttggagaagt ggtgacggtc
    13741 atccgtcaga tgatagacgg tatcggcagc caaagggaag gaacgatgcg tgcggcggac
    13801 agtttcacgg caattgaaga cagcagctat acgattgtgg ataatctggg tgagatgctt
    13861 cagacagtag aagctctgaa aacagccaac caggagatcg tggattccat tcagacaatc
    13921 tcagcgacta cggaagaagt gtctgcccat gccagtgaga cactggaagc ggaagagaga
    13981 aacagagaga ttctgcagaa aattactgaa aatatgaatt ctatggtaaa aaaataagta
    14041 acggttcctg tgacagaacg gacaggatag aaacaggagt gacagaatat gcatgacaac
    14101 ggggtgactt tacagaaaaa ggtgttacag aatctggata taccgtattc gtatgaactg
    14161 gcaaagcgaa tggaacaata ccggtcacat ccgacactgg ggtatcgccc ggcgggttct
    14221 aaggcagaat ttgagaccgg agagatgctg aagacagaaa tggaaaaaat cggactgtct
    14281 gaggttacaa aggatgccgt gacggtagac ggctgggaat ttcataaggc ggcgttaagc
    14341 tatacgaatg cagccgggga gaccgtgtca gcagagctgg gagcctatca gacaaccttt
    14401 gtcaccgatg gcccgaagga attttccatg atgtatctgg gaaaaggaac ggagacggac
    14461 tatcagggaa aagatgtcag ggataaactg gtactggtgg agattaacca gagggatgag
    14521 tggtggatca attatccggt ataccaggca catctgaagg gagcagcagc cttgatcgca
    14581 gtgcagagcg gcggctacgg cgaaatcgac gatgaagctc tgaatgcaca ggatatcgcc
    14641 ggaccggagg atgctcccgc tttttccatt tccagaaaag atgcggccgg cctgctggag
    14701 ctgctgcgtg atcaggagga aataacggtc acctttgatg cggaatccag ggtgaccaga
    14761 aactgcacga cttataatat tgtgggaagg attcccggaa aacatcctga ccgcatggtg
    14821 cttttgtctg cccactatga ctcctatttt gacggattcc aggatgacaa tacagcggtc
    14881 gccctgatgt ttggtattgc aaaggcgttg ctggacagtg gttttcaacc gaacaatacc
    14941 attgtgatct gtgccatggc atccgaggaa tggggcgtgg tggattccaa tttcgactgg
    15001 tctacgggag cttatgagca gatattcacc gcacatcccg aatgggtggg caaggtgatc
    15061 gcagacctga actttgaact ccctgcgttg gcgcatggta ccagagcgag gatccgcagc
    15121 tgctatgaat acgtcagctt tctggaggaa tatctggcgg atctgccgaa tctgacgata
    15181 gcctatccgg aagagacagc agtgacatct ccgatcgaga cctggtcgga tgatttttcc
    15241 atggcgatcg caggtgtacc gtccatggta aatgatttca ccggcggaag ctttatggag
    15301 acccactacc attctcagtt tgataacgat gagttttatg atgaacaggt atatcgtctg
    15361 catcatgagc tgtttgccct gttgatcctg gcactcgatg aaacggcagt ggtaccgttg
    15421 cagttttcac cggtggtgca gcggattcgc aagggcttgg aacagtgtcg ggaaatctgc
    15481 taccgcgccg atgtggcggg gcagctggga gagaaaaaac gggttttgct tgaaaaaatt
    15541 gaggaactgg agactttaag tgaccgggca ctcagaagat gccgggagga atatgaagcg
    15601 gtagaggaat acaaccgtaa ctacaaacag ttgcttagag atggaaaata cgacgaggcg
    15661 gagaaacttt tccggcagat cagaccgctg gaacagaagc tgctcgcaag gttccaacag
    15721 gagcaggacg cttttgtgcg gattgactgg tacggaaatg tgttgtatcc tcatgagata
    15781 tgcagtgcaa atctgagact tctgggcgga gcggtgagaa atctgaagga acagagactt
    15841 tcctccgcac ttcgtaagct ttatcaggta gacaacaatg cctatgcctt taattttgat
    15901 gaagaagtat ataggcattt tacggattat gtattccatc agccgaagga tcgcctgaaa
    15961 tggggttacg gaaggctgcc ggagcatgaa aatctatatg gaacggtcaa gcagcttctc
    16021 caaaaagaaa aacaactgga gatgttgaca ggatccggta tgaaggaaat ggactaccgg
    16081 gaagaaatta ccagcctgga gcatgcatgc agcaagcttg tggtcagcct gatgaatatc
    16141 gtgcaacaaa tggagagatc tgtgacagag atattcgaat tgtaatgatg gcgagagcag
    16201 agcacggaac gatccacaga caacaaagct ggggactgac atcatcataa taaggagata
    16261 acagtaaata caattatgga aagtgataag ttatatcgtg tgcagaagga agaccttccg
    16321 aaagtggaga aaatgctgaa tcagtgcttt gcacacgatc cgttgtacga gacgctgatt
    16381 ccggatccgg aagtccgtaa aaatctgatg ccggagctgt ttaaatgtga catggatgaa
    16441 ttttatgcga cctgtgagat cttcgcagac agcgcggatc tgaacagtgt attggtggta
    16501 tcggatgaag cggagccgta caatgtattt cagttctatt ttacagaggc catggctgct
    16561 ttacagacgg attcctatct gatcaaggag gatccttcgt taaagacatt tcggaatttt
    16621 atgaagggtg aggattatct gaattccagc tggacggatc agctgcatca gaaccaacgg
    16681 ctgcacatca tttatctggc agtggatccc accatgcagc atcacggaat tgcagcggag
    16741 cttctggagg aagccatcga ctatgcccag aagcacaagc tgatgatctc cctggagact
    16801 cataatccaa aaaacgtgga attttatgaa aaatttggat tcaaggtata tggtgtgatg
    16861 gagaagaatt tcccgttaaa gcaatactgt ctgatccgtg aagtacagta atattgctga
    16921 aaatttcaga aaaatccgga aacgcaactg ccggttgaca aaatttcaag ggcacttggt
    16981 agttgtcaac cggctctttt gttatgattt tcctatgtcg acgatctatg tgtgcacaag
    17041 aaaacattaa taaaagaagg agagaatgaa aatgatcctg tcagaaaaaa taataatgtt
    17101 gcgtaagaaa tatgggtggt cccaggagga actggcagaa cgcctggaca tttccagaca
    17161 gtctgtatcc aaatgggaga gcggagcctc aatcccggat ctggaacgta ttgtcagtat
    17221 gagccagttg ttcggagtta ctacggatta cctattaaaa gatgaaatgg aagagacgga
    17281 attcaccgat ggaatgacac cggagatcac agagggcaaa gtgatcacgg tggaggaagc
    17341 caatacgttt ctggaagcta cgaaaaaata tgctgcccgg attgctccgg cggtatcctt
    17401 atgtgtgctc tctcctgttg tattgttgtg gttgttggga atggcaggtg ccaaaagagg
    17461 agcggttaca gaaaatgtcg caggaggaat cggtctcatc gtactgttac tgatggtggt
    17521 agttgcggta gcggtatttc ttctcaccgg aatcccgtat aataaatatg aatatctgga
    17581 aaaagaaaag ctgacattgc aatatggggt gtccggaatc gtggagaaag caaaagaaac
    17641 atttgcggga acctaccgga tctgcataac cctgggtgtt gtattgtgta ttctgggagt
    17701 ggtaccgttg ctggcagtat ctattttttt cgggaataat ggttatgcgg taatccttgc
    17761 tacggatgct ttgcttattg tagtggcgat cgctgtatgg ctgttcgtgt ggtccggtat
    17821 tatctggggc ggttatcaga aattgcttca ggagggggat tatactgtgg agaataaggc
    17881 agttaaccgg aagtatgagc atgtgacagc aatttactgg tgtgtctgga cagcgcttta
    17941 tctggccatc agcctgccga caatgcgttg ggacattacc tgggtcgtat ggcctgtggc
    18001 cggagtattg tatggggcac ttctggcgtt tttgaagata aagaatcgga aagcagagcg
    18061 cgaataagcg gattgcaagg ggaaatacgg acggcgtgaa taaggagcgg aatatatgac
    18121 agtggtatgc cttgtgatac tggcaggaat tttctgggga gattttttct taaaaaatta
    18181 tatagagtcg catgtggacg agcgggaaga acgagagatc tgcagggggg gatttctgat
    18241 ccgcaagcat cacaacaaag gcatgatgct caatgccgga cagaagagaa ggcgtctggt
    18301 ggctgttgtt tcactggcgt ttacactgat attgaccgtt gtctttatca tgagtctggg
    18361 acatcgaggc aacaatttgc tgcgactggg actatcgctg ttgcttggtg gtgccttcag
    18421 caatacctat gaccgccttc gcagaggtta tgtggtggat tatgtgagct tcccggtgaa
    18481 atggcaggcg ttccgcaaga tcgtattcaa ctgctcagat ttctgcatta tcatcggtgc
    18541 gttgctatcg gcacttggaa tgctgtaaat tgagagttag tgaaatacat tggcaaaatc
    18601 cacgaactcc cggagcat
//
LOCUS       DAWDHD010000035        18293 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_211_length_18293_cov_13.014366, whole genome shotgun sequence.
ACCESSION   DAWDHD010000035 DAWDHD010000000
VERSION     DAWDHD010000035.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 18293)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 18293)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..18293
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_211_length_18293_cov_13.014366"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    12369..12468
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5101 a         3918 c         4947 g         4227 t
ORIGIN      
        1 gcgatcgccc tgcggaggct gtgatcgccg aagaatatag ccgttgtgcc aagaatcggt
       61 gataagagag cgagacatcg aaaatcagaa cgacagataa aacaatgcac agggaggtat
      121 ggcagaaaaa tggagcagcc aacaaagaca gtcagtgtag cagcattgcc gcaggtacgg
      181 gtgttgggac gtaccacggg aacggatgtg gtgaacttat tctggaccgg cagcggaata
      241 gaatttatct ataccggcag tgaacttcag atggaggtaa atgccgatta cgacgcttat
      301 gaaccgtggc ttgcggtgga actgaacggc gcgcagatca gtagagttcc attaaataaa
      361 gggaaaaacg aagtgtgcct gttccggggg atgacggtag gaaaaccaaa gcatgtacgg
      421 attctgaagg aagtacaggc aatgcatcag gatcccgggc atctgttgca gattgtggga
      481 ttgcaatatg cagacggtga attcctgcaa ttgccggagc cgaagtaccg gctggaattc
      541 gtgggagaca gcattaccag cggggaaggt acggtgggag ccgtatatga ggaagattgg
      601 atcagtgcct ttttctctgc ggaaaatacg tatccccgta tggtggcaga tgcattatcc
      661 gcggagtatc gtgtgatatc ccagagcggc tggggaattg tcaccggatg ggatggaaat
      721 gcggagaata agattcctcc gttttacaca caggtctgtg gattgctcac tggggagagg
      781 aatgcttctt tgggagcgtt ggaagactat gatttcgaag catggcagcc ggatgcggtc
      841 attatcaatc tgggaaccaa tgatgccaca gcgattcagt ccgcagcgga actggggcag
      901 gaatgggcag gaacacggga catagaagaa gttaaagaga tcctgacaac agctatctgc
      961 gatttcctga aagtagtgcg gaacagtaac cccacagcac agatcatctg gggttacggt
     1021 atgctgggag acaacttcct gtccgccatc cgggaagctg tggaaggcta tgcgaagcag
     1081 accgccgatt cccggataca ctttttacaa cttcccgaca ctacaccgga tactgtgggc
     1141 agccgcctgc atcccggcgt gaaagcccat caaatcaccg cgaaagagat cgaaacatat
     1201 ctgcaagaac tgttatagaa gaaatttata atttcttaag aaaaatgtga cgtaaaatgt
     1261 gacaactccg cgctatattg aaacccatag aaagtaattc atttggaggg gctggcaata
     1321 tgacgcagga aaatcgttca agcagacagg cagagatcca tataacgcag atgcggagga
     1381 atccgtatct ggtggattat caaaccggga aaaatatggg cagacatata ggaaggcgca
     1441 cagaacggtc acaacacagc acacctgcaa tgccgcagga gggcacaagg acaagaacat
     1501 atcctcagca cagtgtagca ccccggcagg aaaatagaac ccaccagcag aagcagcgaa
     1561 ctgcggatgc ggtatattgg gaaaatgtat acaaagacaa tacctacaat acccgtgcca
     1621 ggcaccgtct ggcgagggcg gagagacagc gccgccgcag acgggcaaga atccgcagat
     1681 atatccggct gactctgctt agtgcaggaa ttctggtagg aatcattgcg ttgacaaagc
     1741 ttctgaatgt gcctgccggt actgcggaag ccagcacacc gatggaagca atagacatgg
     1801 cgcagcaggt ggaaaaaggg attgagaata ctataggaac ccggattcag ggactgtcgg
     1861 cggagactta cgcagcgcac cctgactgga cagaggattt cctcacggta aatgaatatt
     1921 ccagaccggg agatcctttg acggaggtga ataatatttt cgtgcattat actgccaacc
     1981 ccggcaccag tgcagcacag aaccgcagtt atttcgaaca gcttaaggac aatcatgagc
     2041 gcagtgccag tgcgcatttt atcatcggct ataacgggga gatcctccag tgcgttcctc
     2101 tggatgagat cgcttatgcg gtgcagaccc gtaacgagga ttccatctct atcgaatgct
     2161 gctataaggc ggacaacgga cagttcacgc aggaaaccta cgactccctg atcaggctgt
     2221 tgagatggct gatcgacgcc tatgaactgg aaccggagga tattctccgc cattatgact
     2281 gcggtgggaa aaaatgtccc atatattata cggaacacga ggatgcctgg gaccggttaa
     2341 aagaggatgt caaaaatcta tagaaaagag gaacttttcc cgaaaggaaa ggttcctttt
     2401 agtttgtctt gttttaagaa tgcaagtttg ttagaataga cgcatatgga atacgatttc
     2461 ctattcattg ggaaaagtgc tgcagacaaa gccctaaagg gcagaaatga ggagtatcat
     2521 gaatatttta tcaccttcaa ttctttcggc ggatttctgg aagttgggag aagatatcaa
     2581 agctgtggaa ggagccggcg tgccctggct gcatgtagac gtgatggacg gactgtttgt
     2641 gccttcaatc tcttacggaa tgcctgtctt gaaggcagtg agaccccata cagacatgtt
     2701 cctggatgtg catctgatga ttgagaagcc ggagcgttat gtggaagagt tcgctgcctg
     2761 cggcgcggat ctggtgaatt tccatctgga agccacagag gatgtgaagg gatgcattga
     2821 aaaaatacgt gccaccggca aaaaggtcgg catcaccatc aaaccagcca ccccggcaga
     2881 ggcagtggag ccttatctgg aactggtgga tatggtactg gtcatgaccg tggagcccgg
     2941 tttcggcgga cagaagctga tcccggaatg cctgaataag gtagcggtga tccgtaaaat
     3001 gatcacggac agaggactgg ctaccgatct ggaagtagat ggcggcatta atcttgacaa
     3061 cgtagcgctg gcacttgatg cgggagctaa tgttatcgtg gcaggttctg ctgtattcaa
     3121 ggatgacatc gcggccaatg cgaaggcttt tcttgcgaag atgcagtaac atcgccatag
     3181 gcaggacttg ggcggggaac gtcttatgga aggcgcagac tgtcggcaga cggtgacgga
     3241 aggatagaca gttgtctgcg gaatggagat tgatatggag ttacagatat tacacgcaat
     3301 acaagaaatt catcagggat ggctgacgga cgtcatgcgt ctttttacca ccataggaga
     3361 aagcgggctt gtatggattg ccatcgccat cgtcctgaca tgtataccga agaccagaaa
     3421 atgcggtctg actatgatga tcgcaatggc tattacctat ctggtgggga atctgtttct
     3481 gaaaaatgtg atcgccagac ccagaccctg cgcagtcgac aacagtgtca tattgaagat
     3541 cccgtttcct tctgaatatt cgttcccttc gggacacagc tccaatggat tcgccggtgc
     3601 agtgacgatc ttctgttatt atcgtagagc aggactctta agtctgctca tggcggcact
     3661 tattgcattt tccagacttt acttcttcgt tcattacccg acggatatcc tgggcggaat
     3721 cttattggga acgctggatg cgctgttggc ggtctggctt gtgaaacact tggagaaccg
     3781 tgcagatgcg cagtctgcat cgaacgcaga taaaaccgaa cacgtgcagg aaaaataaaa
     3841 taacgggaca gaaaccggga tcagacagaa agggcaaaaa ctttgatgaa cagcagaacg
     3901 ttatggaatg ataattggta ttttgccaaa acggaattag gtgtgaaatg ggaagacagg
     3961 cagatctggg aagccaaaat gcagccggtg gatctgcccc atgactggtt gatctatgat
     4021 acgaagaatc tctatgaaga cagcatcggc tggtatcgga agaactttgc ctatgccccg
     4081 gataaaacag gaaatgatga ccgtgtcatc ctccgctttg aaggtgtcta catggattct
     4141 tctatttatg taaacggtga gcaccttggc gactggaaat acggctattc tacctttgac
     4201 tgggatatta cggatgcttt acataaggga gataatgaaa tcgttctgcg tgtcgtattt
     4261 caggcgccca acagcagatg gtacagcggt gccggaatct atcggaatgt gtggatgatc
     4321 cggctgccca agacacatat tcctctggac ggcatctata catcttctgt tgagacaaaa
     4381 aatggttatg atctgaccat cgacacggaa ctggaatggg gaacagatag tggaaattat
     4441 gaactggaat actgtctgcg agatgctgat aacagcaaaa gtcccataga aacagaaaac
     4501 caggagctct tccgggtaaa acagcctctc acctgtggac agaacaagat atccataacg
     4561 atccctgtgg ataatcctag aaaatgggac attacggatc cttacttgta tgatctgcag
     4621 gtatgcctgt ggagagagca ggagaccaac agccgggaac tatgtcagca agaacatttc
     4681 cgtatcggtt tccgcaccat gcaatttgat cctgccaggg gctttttatt aaacggcaga
     4741 aaagtgcgcc tgaacggtgc ctgcgatcac catgacctcg gagcactggg agcggctttt
     4801 cacaaagaag ccatgcgccg gaaatttgaa ctcatgcgtt ccatgggtgt caatgctgtg
     4861 cggacttccc acaatatgcc cgctgtggaa ctgatggaac tggcggatga gatgggattt
     4921 ctgatcgtat ccgaagcgtt cgatatgtgg gagatggcaa agaatcctta tgattatgcc
     4981 agatttttca aggagtggat ggagaaggat gtacgcagct ggatccgcag agaccgcaat
     5041 catccttccg ttatcatgtg gagcattggc aacgagattc cggataccca tgcggatgcc
     5101 catggacagg agatcacccg tagcctcctt gcagcagtca gaaagtggga ttaccggaag
     5161 gtggcaccgg tgacgatcgg ttccaattac atgccctggg aaaatgccca gaaatgtgcg
     5221 gatatcgtga aagtggcggg ctataactac gcggaaaaat attatgcgga acaccatgaa
     5281 aagcatccgg actggatcat ctacggaagt gagacttcct ctgtggtaca gagccgaggg
     5341 gtgtatcatt ttccgttgga gagggcgaat ctgatcgaag cggacgaaca gtgttctgcg
     5401 ttgggcaaca gtactaccag ctgggcggcg aaaaatacgg aatactgtat cactatggac
     5461 agagataccc cttattcctg tggtcagttc atctggacag ctattgacta tatcggtgag
     5521 ccgacccctt atcagacgaa aaacagctat ttcggacaga tggatacggc gggatttccc
     5581 aaagactcct actacgtctt ccgttcggaa tggacggatg ggaaaaaaga tcccatgatc
     5641 catgtatatc cctactggga ttttaatccg ggacagcagg tggacgtgcg gatcaccagc
     5701 aatgctccgg aggtggaact gttcgtaaac ggagtgtcac agggaaaaca gcagatcgat
     5761 catcagaaag gaacggtatt gcagccttcc tggaaagtac cgtatgcgcc cggcagtatc
     5821 tgtgccgtgg catacgacga aacgggtagg gagatagcca gacaggagag acattccttc
     5881 ggcaacacag atcattatgt attaaaagca aataaatcag ccttgcatgc tgacggcgag
     5941 gacatgatct ttgtggagat ctcagcggag gaccgggacg gatatcctgt ggaaaatgcc
     6001 tccgattatg tgagagtgac cgtggaaggt gctggaagac tggtgggact ggacaatggt
     6061 gacagcgccg attacgatgc ctacaaggga accgtacgta agttgttcca ggggaaatta
     6121 ctggctatga tcgcagcaaa aactacgccg ggggagattc gtgtgactgt gaaggatgca
     6181 tgggcagcca cagtgaacag catgaccaat gaaaccggca ctggaacagc aaaggcagct
     6241 gccgggcacc gcacggcgac agtgacgtta caggcagtcg aagcaccgat tcgtcccggt
     6301 gtctgcgcaa cagaagaaaa cagagaatat ccgcccgcct ttgtggatcc cggatttgtt
     6361 ccggtacgga aactggaaat gagcgctccc tatatgagat tgacaccgga gaagccgtat
     6421 gtattgctac atacccgtat ctatcccatg gaagccacag accgtaagct tctctggagc
     6481 attactgacg cttccggtat ccccagccct gtggcaaaac tggaggaact ggaagacggc
     6541 gagagcatcc ggatcacagg cgtcagtgat ggcagttttc aggtaaggtg tatgacctgt
     6601 aacggaacaa ccaatattaa aatgatttcc caacttgact ttgcagtgga gggcatggga
     6661 cagacgttcc attctccgta tgagccggtg acgggtattt cttatgtcgg aagttttggc
     6721 ggagacgtaa gccgcggagt ggagaacagt gccggaacgg acaaggcaca gcctaccgga
     6781 ctggtctatg aagcgttaga ctttggtgaa ttcggaagtg acgagatcac gctgtccatt
     6841 tttgccaacg ataatgatcc ccaccggatt cagatatgga agggagagcc cgaagagagc
     6901 ggttccggga cacagggaac actggtgggt gaaataacct atcagaagcc gggcatctgg
     6961 gaagtcttcc agccggatac cttcgtattg ccccggagat taaagggtgt aacaaaactg
     7021 acaatagtgt cagaagataa aattttcctg aaagagttcc atttcaccag acaggaaaaa
     7081 gcttatgcaa gactgagtgc tctcacggac ggagttacct atggtgacag ctttgtgaga
     7141 accggggatg ccgttgaaca gatcggcaat aatgtctctc tcgaatttac ggatatgaac
     7201 tttggagaag cgggaaccga tagcattgtc atctgcggaa gaactccttt acagcagaat
     7261 acgatccagc ttaggtttac cgatggagct acacaggtac ttcccttccc acacagcgag
     7321 gaatataggg aactgcgttt ccctctggaa agagtgacgg gagagcagaa tgtgaccttc
     7381 gttttcctgc cgggatgcaa ttttgatttc aaatggttcc ggtttgaaaa aagcagatat
     7441 gaggagtaag cgataactat gagcaaatgg aacagaaaaa tatgggcagc aatactggcg
     7501 gtgggaatga cagcgtctct gactgcctgt ggaggcagca aaggagaaaa cagcaatgcg
     7561 tctgctgccg gtacacagaa tgagactgcc accgtacagg atgagagcac ggacagtaac
     7621 actgacagca ccggcagtgc cacgtctctg gtgcaggaca accgggtact gcagatctcg
     7681 gacgacaact accgtaccta ctatgagctt ttcgtatatt ccttctatga cagcgacggt
     7741 gatggcattg gagatctaca gggcgtactg gaaaagctgg attacctgaa tgacggtgat
     7801 gataccacag atatggatct gggaatcaat ggtatctggc tcatgccggt gatgccctcc
     7861 gtcacctatc ataaatacga taccacggat tatgagaata tcgatccgca gtacggcact
     7921 ctggaaacat ttcaggagtt gctgacggcc tgtcatgacc gtgggatccg tgtgatcctg
     7981 gatctggcaa tgaatcattc ttccagcagc catccctggt tccaacaggc tgcggagtat
     8041 ctgaagagcc tgccggaagg ggcggaaccg gatagttccg aatgcccgta cgtggattat
     8101 taccattttt ccagagaata ccagggcggg tatgcacagc tggatggaac ggcctggtat
     8161 tatgaagcga gattctggga cggaatgccg gatctgaatc tggagaatga ggcggtacgc
     8221 cgggaatttg agcagatcgc ggacttctgg ctggatatgg gtgtggatgg cttccgactg
     8281 gatgcggtga aagagtatgt taccggatcc acagaggaca atgtggaaat tctaagctgg
     8341 ttcgcggatt atgttcatgg caaagatccg gagaattatc tggtgtgtga atgctggacg
     8401 gatcagaata cctatgccaa atattatgag agcggcgtgg acagcatgtt tgattttgct
     8461 tttgccgaca agtccggtat cattgccaat gtgatgaacg gtaaggcatc ggcaacatct
     8521 tatgcaaaga atctggaaac agagcagggc atgtatgcgc agtacaatcc ggattatatc
     8581 aatgctccct tctacaccaa tcatgatatg ggacgcagtg caggctacta tgccggggag
     8641 aatagcgaag ctcagacgaa aatgggcaat gcccttaatc tcttaatgaa tggcaatgta
     8701 ttcctctatt atggggaaga actgggcatg aagggctccg gcaaagacga gaacaaacgg
     8761 gcccccatgt actggagcaa ggacggcaat gcggaaggca tgtgtaaggg cccggcagat
     8821 atggatgatt tccggatgaa atttgacagt ctggaagaac agcaggagga tcccgattcc
     8881 atctataatt atgtgaaaca ggtgatccgt gtccgcaacc agaacccggt gatcgccaga
     8941 ggcaccaccg cttatctgga gcagtattcc ggtgaacaat gctttgccct gacaagaagc
     9001 tatgaaggca gtaacttatt attgttggga aatacctctg cggaggctgt gagcgtggat
     9061 ctgaccggac ttaccgtagg aggcaccaca gcggaggaac tggtactgct gggggaactt
     9121 tataccggag aagagaccgc ggagcgtgcc ggtactgtag ttacgcttcc ggcttattcg
     9181 atcctgatcc tgggagaaga ataagacgag caaaacagaa tacaggaaca aaaacaggtc
     9241 tgtgggcgaa ccctacagac ctgtatgctt tcttacaaaa tcttcaaatt cgtttaccgg
     9301 taacggcttc gagaagtagt aaccctgaat atcatcacag tgaagattct tcagaaactc
     9361 tacctgatcc gaagtttcta caccctctgc ggtaatagtc ataccgaagc tctgcagcag
     9421 ctgcgtgata tgcaacagca gcttttcacc gttccggtca ccaatgtaat cgatcagact
     9481 cttatccagc ttgattgtat cgaaatgcat gacattcagc gaagacagag aagaataacc
     9541 gcttccgaaa tcatccagca gtaaagtaaa tcctgcttca tggaactgct cgatcaggga
     9601 ggaaatatca ctgttatcaa tggtagcact ctcagtgatc tctaacggaa catatttcgg
     9661 atcgagccgg aatgaattca aaatctcccg gtacttatct accacattgc tgtagtatag
     9721 gctgactctg gaaatattta cagaaatcgg atacaatgtt gctccggcct gcagccatct
     9781 ctgctgttgt gcgcacactg tgcggaacac atattcatcc agtgttgtga tattaccgtt
     9841 tttctcaaat aatggaatga acttcccggg ggagagcatg gtgccgtcgg ggcgcttcca
     9901 acggatcaga gcctccgctc ccagaatgtt tccggtgacg gggtctactt tcggctgata
     9961 ccacagctgg aattctccgt tcttcagggc gtcttcgaag acatcttcca tcagacgatc
    10021 ctcggagagc tgtttgatat ccagatcttc ataaaaagca taatgcctgg tacgttttcc
    10081 ctttatcgga tacttagcct gtacagcctt gccgtaattc tgctcgattt ccttgtgatc
    10141 ggcagtctca tagacaccgc acagagggaa cagtttcgga atattaagct ttgtggacaa
    10201 attggaaata tcctgacaca ggcactccaa tctgggttct aaggtacacc ggtcattatc
    10261 ccgcaggaac aggatgaacc ggtctgcatt gatgtgggca gaaaattcac ccagatgtgt
    10321 accggtttgc aggagcttcc atacttccaa cagtgtttcg tcacccttgg ccacaccaca
    10381 ggtattattg atcagcttga aatcctgaag atccagggca acatagaagc cgctgtctcc
    10441 gcctcggtgg gataatttct ccttgaagca ggcaaaatta tcaccatttg tcagaggatc
    10501 cacataagcc agacgatata actgcctgcg ccgtgtacgg atactgtgca caaaagcaaa
    10561 agtaccgact ccggcgatca caatgatcag caggatcagc agccgcacct gaaacaatat
    10621 cggagcggaa cgactggaaa gtacctgatc gggaacaata gtcagggcga accactgtct
    10681 gtcgctgcgg agattgtcca ttgcagccgc atagtaataa tacttctgac tgccttcata
    10741 gaaataacca ccgttggagg aagtactctg aagcagcgta tgcagatcct gcaaaggctg
    10801 gtcgttacgg gcattctgag tcagataatc ggacatacgg tcggcttcct gcagggaagc
    10861 cggtgcattg gaagatgcgg cgatcaggtc accgtcctga tttaccagac aactgaatcc
    10921 ctgattatta aaggattgca cgcctaaaat ttcttcaaaa tgctggttcc gataggtggc
    10981 aaataaaaca ccgataatgg aatcactctg atcatatacg ggaatgctga atacattgat
    11041 gggctccgga gtacccattc gatcctgaag agtattggta ataccgggaa gcccctgcat
    11101 accatctttg aaaaaatctc tgctggacag atcttgtaca tagccgtcag tggccattgc
    11161 ctgaccatcc ggtgagatga agccaatccg cttaaaccca taggtggaga cgaaagattc
    11221 caactgtgcg gcaatggagc tggcattggc tgtacccggg gaaacactga tcgcatcggc
    11281 aaggctgtat aataagttga agttttcacg gatctcctgc tgtacgatca gggcgttctg
    11341 cgtggcaaca tcccggagag tggactgtac ttcttcctct gtatcctgtg tgatctgggc
    11401 agaactccac atgacggtgg aaattaccag taccagagca atgcccagaa tgatcaaggt
    11461 ctgctttggc aatttacttt gtttcatttt atatcctctt atgtatgtta taaaaataac
    11521 cttatataat atactattat acacctttac gattgcatgg caacaaaaac tgcattgaca
    11581 gtccggattt cctgtgctac tgtaagaata cacaaatgca atgaggaaga gagtatctat
    11641 tttatagaag ttttacagag aacattccgg caggctgaga ggaatgaaac ggacagatgg
    11701 agaagatggc ttctgagcgg cgcaccgaac ggacaccggg acagaacaaa cggtgaaagc
    11761 aaggctgcga caggatccgc ctgttacagc ggatagggtt acggacccga agaggtctgt
    11821 actgtgaggt acagacgaag agaagtggta acacgagata acgctcgtct tcttgaagaa
    11881 cataaggttc tttgaggaga cttttttaat gccaacaagg tcacaagaca gaaattacga
    11941 tgtgcttgca cgagagtaat tttctgtctt gatgacctgc cccgatatga gcatagaaga
    12001 gaggcgtaag cctttcgata tgcgaatggc gggtaggatt gcgggagcgc aaagcgcgga
    12061 gcaatccgtg tggcattaga tagtgttata ggagtagaaa aggagaacag acacaatgga
    12121 aagcaaagga aaatattatc ttaccacagc gattgcctat acatctgcga agcctcatat
    12181 cggcaacaac tacgagatcg tactggctga cagcatcgca cgtttcaaga gaaaggaggg
    12241 ctatgatgtc ttcttccaga ccggtactga cgagcatgga cagaagatcg aactgaaggc
    12301 tgaggcagcc ggcatcacac ccaaggagca tgtggacaag atcgccggtg tgatccgcgg
    12361 actgtgcgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnat cacacccaag
    12481 gagcatgtgg acaagatcgc cggtgtgatc cgcggactgt gcgacctgtt aaatatttct
    12541 tatgataaat tcatccgtac taccgatgag gatcatgaga agcaggtaca gaagatcttc
    12601 aagcgtctct atgatcaggg agatatttac aagggggcct atgagggaat gtactgtacc
    12661 ccctgtgaat ctttctggac ggagtcccag ctggtagacg gcaagtgccc ggactgcggc
    12721 ggtgtcgtaa agcctgccaa ggaagaggct tatttcttcc gcatgagcaa atatgcagac
    12781 aaactgatca agcacattaa tgagcatccc gagttcattc agcccgtttc ccgtaagaac
    12841 gagatgatga acaacttcct gctccccgga ctgcaggatc tgtgcgtatc ccgtacttcc
    12901 ttcacctggg ggattccggt gaccttcgat cccaagcatg tcatctatgt atggctggat
    12961 gcgctgacca actacatcac cggtatcggc tacgatgcag acggcaacag caccgaacag
    13021 tataagaagc tgtggcccgc agacctgcac ctgatcggca aggatatcat ccggttccat
    13081 acgatctact ggcctatttt cctgatggca ctgggcgagc ccctgccgaa gcaggtattc
    13141 ggacatccct ggcttcttat gggcggtggc aagatgagca aatccaaggg aaatgtggtc
    13201 tatgcggatg atctggtaga ttatttcggc gtagatgcag tacgttacta tgtactgcat
    13261 gagatgcctt ttgaaaatga cggtaatctg acctgggaac tggtagcaga gcgtaccaat
    13321 tccgatctgg ccaacaccct gggcaacctg gtgaaccgta ccatttccat gagcaacaag
    13381 tattttggcg gtgtagtaga gaataggaat gtacagctga ccgagaacga tgcagcagtg
    13441 gatgccgatc tgaaacagac tgtgaccggc acttatgcaa aagtagtagc caaaatggaa
    13501 gaactgcgag tagcagatgc cctgaccgag atcttcggca tcttcaaacg ctgtaataaa
    13561 tacatcgatg agaccgaacc ctgggtactg gccaaggacg aagccaaggc agatcgtctg
    13621 gctaccgttc tgtacaatct ggtggaaggt attatcattg gcgcatccct gttagagcct
    13681 tatatgcccg aaaccgcaga gaagatcgca aaacagctga atacctccct gcgtgacttt
    13741 gatcagttag agcagttcgg cctgtatgag agcggcaaca gggtgacaga tcagcctgag
    13801 atcctcttcg cccgtctgga tatgaaggat gtagcaaaaa aagaggcaga gctggaagaa
    13861 gccatgatca aggcagcagc ggagcatgaa gcggaagagg caaatgccga ggcagaaaag
    13921 gcagagcagg aagccatcga tattgagccc aagacagaga tcgaatatga cgacttcgca
    13981 aaaatgcagt tccaggtagg tgagatcatt tcctgtgagg cagtgcccaa gtccaagaag
    14041 ctgctgtgca gccaggtgaa gatcggcagc caggtacgtc agatcgtctc tggcatcaaa
    14101 gcacattaca gcccggagga aatggtgggc aagaaggtta tggtactggt gaacctgaag
    14161 cctgcgaaac tggcaggagt aatgtccgag ggcatgatcc tgtgcgcaga agacgcggaa
    14221 ggcaatctgg ctctggtcac cccggagaag aatatgcctg ccggagcaga gatctgttaa
    14281 atagtatatg acaacgtaac tttacgaatg aggctggatg aactgttact attttacaaa
    14341 aaaagcaata tagggcttaa aattttataa gaaatgatat ataatgaata ggatgccaaa
    14401 gcagatgatt gtaaaatgga catttgtttt gtgcgtccta tgattttccc gtaaccaagg
    14461 aggtcctatg attaaggaag aattttactt tgattcccgg gacggcgaaa gccgtatcca
    14521 tgcagtgaga tatactcccg atgacggcaa tgtaaaaggc attgtgcagg tagtgcacgg
    14581 aatggcagag tatgtggagc gttatgagaa cctggctgaa tttctgactg ccagaggaat
    14641 ccttgttacc ggagaagacc atctcggaca tggaaaatct gtggcggaag gcggcactta
    14701 cggttacttc tgcgaacagg atccggcaac ggtagtggta agggatgtac atcggctgaa
    14761 gaagatcaca gaggaaagct atccggaggt gccctatgtt attttgggac acagtatggg
    14821 ttcctttatt acgaggaatt atctgtgtcg ttacggtaag ggagtcgacg gggcggtaat
    14881 tgtcggaacg ggaatgcagt ccgggggatt gatcctttgt tccaaagcca tggcaggcat
    14941 acagaagctg ttctgtggtt caaagcatat cagccgtttt attgacaagg ctgctttcgg
    15001 cggctataac aagcggatcg atgctccgag gacatccagt gactggctga gccggaatcc
    15061 ggagaatgta gaccgctata tagcggatga gctgtgtgga tttaccttca ctgtgaatgg
    15121 gttccagacg ctgtttgaac tgatccgcag actgcagaag caggagaatc tggagaaggt
    15181 accgcagaac ctgccaatcc ttatggtatc cggtgccgag gacccggtgg gaaattacgg
    15241 taagggtgtc cggaaggcat gtgattctct gaagaaggct ggtgtgaaaa atattaccgt
    15301 caaactgtac gagaatgaca gacatgagct tttgaatgag gaagacggtg aacaggccat
    15361 ggaggacatc tatcaatgga tccgcaggga gattccggga ctggattaag accggaaagc
    15421 tttcggatca gagaggcggt gcatcatgtc ggatacggca gcgggcactt acgagatcag
    15481 atgggcggaa gacaaagact ggattcctgc catgcatatg atctggaaga catttttgaa
    15541 atttgaggcg gtggattata cagaggaggg aatccgtaac ttttatgatt ttattacgga
    15601 tgaacacctc tataagtcgt tcctgcaggg aagataccag atgatggtgg cgctggacgg
    15661 tgagagagtc atcggagcgg cttccgtgcg gaattataat catttatcac ttctttttgt
    15721 tgatgaggag tatcatcatc ggggaatcgg acgggatctg atgagaagaa tgtgtgaata
    15781 tctgaaaaaa gaggaagggg aacggtatat gtcgctgaaa gcagctccgt atgccgtaga
    15841 gttctatcga aaactggggt ttcatgcggt acatgaagag gaggcatttt ccggaatacg
    15901 tgtgaccccc atggaaaaat ttttataaaa cagaaaagga taagatgtaa atgaaaattt
    15961 ttgacctgga tagcccttta atgaatgtac tgaacaaaat ggcagatctg atgtggctca
    16021 atattctgac actgatctgc tgcatccctg tcattaccgc tggagctgcg ctgacttcca
    16081 tgcattatgt ggcattgaag atcgtgcgta acgaggagag ttatattaca agaagctttt
    16141 ttaaatcctt taagaccaat ttccgccagg caaccctgat ctggttgctg cttatgctgg
    16201 tagcggcgat ccttggcgga gattactaca ttattaccaa atccggaatg cagttcagcc
    16261 aggtgttggt ggtactgatc atggcagcag gagtgctggt gatctgcact tctttatatg
    16321 tatttccggt acttgccaaa tttgacaata caatcatggg aaccattcga aatgcattta
    16381 tcatgagtat tttacagctg cccaagaccg ttgtaatgtt cgtaatggca tttttcccgt
    16441 tgattattta tctggtatct ttaagactga tacccattat tttcctgttt ggtttttcgt
    16501 tgccggctta tgcctctgcc atgctgtaca ataaattctt ccagaagctg gaagatcagt
    16561 tgttgtcaga acaggagcct gcaccggaaa tctcgccgga agaggatgag agagtattcc
    16621 atgatgaact ggatgaaagc ctgaaaacag acgtgaagta acaaaaatgt cctgcgggag
    16681 catcagacga gaggtgttcg gagtatgcaa aagagaaaat tttgggaaat tgttttccag
    16741 tgggtctttc cctgcgcatt gctggttgtt gcactggttc tgagtattta cgaatttaat
    16801 aataaaatga attcagccat acagacggtg gtggatgatc aggcagagca gatcggctat
    16861 tactattccg cgggagtaga tgacaagctg ggggcattgg gagatatcac gagtgccatg
    16921 gcaaacatta tggcgagccg gccggacaga agtgatgctt ttgtatatga gaagctggac
    16981 accattgtaa aagccagcaa cgcctatatg tctgcttatt gtgctgtgaa cggaaaagga
    17041 atgctgagtg accgccggga gttcgatatg tcggagttaa actattacgg cagcatcagc
    17101 ggaacctccg cccattatat ttatgccggc acggatggga tcaacggaca gactgccttt
    17161 atctatgtgt gtccgattgc gatatccgga aatgtcaccg gatatctgct cagttatatg
    17221 aacccggatg acatgaagga gttcttcgac aattctgtat atggtgacaa ggcatttttc
    17281 tctctggtca accggaatgg aaccattatg gcatgctacg gtgccactga cggaacggct
    17341 attttgcaga atgatttctg gaattctctg aaagatattt cggaaagcct gggaagctgg
    17401 accctgtttg aaagacagca gcagaagggc aacagcggca ttttacatgt taaggaagac
    17461 ggtgtaggca agatcatctg tcattttcca atccgcggta ccgagtggaa tctggtggtc
    17521 ggtattgatg aatcctatct gtctggaatc cagcagtcct tccgcggccc gattgtgaat
    17581 ctgaccgtca agatcagtat ttccattgca gtagcgctta ttattatcac cgcaatcaat
    17641 attctggtac gcatcaaagc ctctgaacac agtaaggtgt tggaggataa agcggatacc
    17701 gacctattga cggatctgaa caataagatg gccacagagc gtaagatccg ggaatacatg
    17761 gaaaagtacc cggacaagca gggcgtattg ttcgtactgg atgttgataa ctttaagaag
    17821 atcaatgata ctatgggaca tgccttcgga gatgaagtgc tccgcagcct ggcagtacgt
    17881 ctgcaatcca tgttccgggc tacggatatc gtgggtcgta ccggtggcga tgagttcatg
    17941 gtgtttctga aggatatccg cgagatcagt atgatcgaac gggaaggcaa gaagatcgaa
    18001 cagttcttcc atcagtttga agtgggagaa tatgtaaaat attccgtaac ggccagcgtg
    18061 ggagcagcgg tcttccccgg ggacggcaag tctttcgagg aactgtacaa agccgcagat
    18121 aatgcgctgt atgtgtcaaa aagacatggc aaaaaccagc ttagcttcta taaaaagccg
    18181 gaaaatgaga caaagtaggg cgattcttgt ggaatatgtc caagaaatgc gggcggattt
    18241 gttcaatctg acaagcattt cgaaaaaaac caaactttat aagcaattct tac
//
LOCUS       DAWDHD010000036        17573 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_215_length_17573_cov_13.368821, whole genome shotgun sequence.
ACCESSION   DAWDHD010000036 DAWDHD010000000
VERSION     DAWDHD010000036.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 17573)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 17573)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..17573
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_215_length_17573_cov_13.368821"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    8656..8755
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4948 a         3489 c         4754 g         4282 t
ORIGIN      
        1 agtgaggaat gtaaagacat ccttatgaag gagattaaga aaaattattt atagataatg
       61 aatatagaaa tacagggaaa atgaaacttt atgatttgca gtaggtgaag cagatgaaga
      121 taaactggga aaggtactgg agaatccgca ggtaattaaa acaccggtag taagaaacgg
      181 aaagcaatct acacttggat atcagcctga tgtatggaag ggatggaatt aatggtaaag
      241 aagataatgc atgatccgct gtttctggca cagaagtcag tggatgcaac agaggcagat
      301 aaacagattg tgacagattt gcttgataca cttaaggcaa atttagaaca ctgtgttggt
      361 atggctgcca atatgattgg agtaaaaaag aatatcattg tagtagcagc aggcccgttt
      421 cagtttgcta tgatcaatcc ggtgatcacg aaaaagctgc gtccgtacca gacagaggaa
      481 ggatgtctgt cactggaagg tgtgagaccc tgcacgagat acgaagagat tgaagtggat
      541 tacctggata gcaatttcag gaaacagcat ggcaaataca ccggctggac agcccagatc
      601 atccagcatg aagtagatca ttgttgtggt gtggtgatat aacattggca agtcaggtat
      661 ggaagtttat cacacacaat gattggaata actcctgtaa actttacctg aaacggtatt
      721 tctttccatg cagtatgcta atattatcct acagcaagga gagagcaaga gagcccatag
      781 gatgaggaac aaaaatgcag gtacgtgaag tgatttgtcc ttcctgcagc catcgtttca
      841 tgtggatgga tgtcgaagag ggaccgacga tcaaaaaata ccggtctgta gagaccggag
      901 agacattcca tacggcagta tgccctcagt gcgggaaacg cgtgatattg ttaccggaca
      961 ggatatgcgc agtgttgccc ggaacggaag ataacctgga aatgatgtgg gagagaggac
     1021 tatgaaatta tggctggcag aacgtgagac cttttatccg gtggagatcc atacggccaa
     1081 cccggtgggg agagccaaca tggagaggat tctggccaga tactggggag aacagtactg
     1141 gtaggaaatt ttgtgataat ttaaccaacg gttaataggc aaaagagatt ctttgtgcta
     1201 tagtaaaagt ctacggaaag acggatatgc aactggatgc tgtacggcat cggaaggagg
     1261 cgtatgatgg aaagaccgca ttttgacatt gtggccagcg gcgcacggat gagagcaatc
     1321 cgtcaggagc ggaagatctc cgtgaagcag gtcatggaat acatgggctt tgaatccacg
     1381 caggccgtgt ataaatggga agccggaaag tgctttcctc aggccgacaa tttgattgct
     1441 ttagctattt tgtatcgtgt aagcccgatg gagcttttgg tagaagagga ccttgtgccc
     1501 tcttctagtc atatctgggg aaatgtcgca tagtgcggaa tggaagagaa ggtgcacagg
     1561 aagagcagta tgatttacat aacgggagat acccacgggg atttcacaag attttccgcg
     1621 aaaagattac ggagaaccgg catggaactg acaaaagaag attacatcat tatctgcgga
     1681 gattttggac tgtgctgggc gaaggataaa acttttgagt atcattgtaa taattttgcg
     1741 gagaaacctt acaccattct gtgggtacag ggaaaccatg aaaactatga tatgatcggg
     1801 gaatatccgc tggaagaatg gcatggcggg aaagcgcggc acattattcg ggacaaggtg
     1861 atattgctgg aacggggaca ggtcttcgag attgacggca agaccttttt caccttcgga
     1921 ggggcatctt cccatgacat tcagggagga attctggatc gtcagacggt ggatttcgca
     1981 gaacagaagc gcagggcaga ccggaattat cttccctacc ggatccttca ggaaagctgg
     2041 tggccccagg agctgccgac agagggagaa ctgcaggagg gcctgcgcaa tctggaacga
     2101 tatcactacg aggtagatta tgtgatcaca cactgttgtg ccagcacgct gcaggatcgg
     2161 atcaatgccg ggacgggacg ctcctgtgct ccggatctgc tgacagacta cctggagact
     2221 ttggaacaaa agctgcatta caggcattgg tatttcggcc attatcaccg ggactgtcag
     2281 ccggatgaca agcacacgct ggtatactat gcgatactgc cgctggaaca gaaggaaagt
     2341 gcagttgcag tgccagtgcc gggacagatg ggaggagaaa cggatcatgg caagggatga
     2401 gaatgttaga atttttcagg atacagaaaa acgggtaaag gaagatccgg ggctgcaaga
     2461 ggcagtgaag cgctctgtgg cagaacaggt gctgatcccg gagatgatgg aagtcatgga
     2521 cctgatgccg gagcttgcac agaatcgtga cagatatgag aaggatgcgg aaatcatagt
     2581 gtcgaagaag cgaagctatg aagcagctgc cgggtatccg ggagaaaggg tatgtgttca
     2641 caattttgca tcagcgacga atcccggagg tggtgtgacc aagggaagct ctgcccagga
     2701 ggaatgcctg tgccgttgtt ccaccctgta tttttgtctg aatacgaagg aaatgtgggc
     2761 aggcttttat tctccacaca gatatgcgca ggatccgatc cacaatgatg actgcatcta
     2821 tacgccgaaa gtcattgtat tcaaatccga tactgcgaca ccggcaccgc ttccggaatc
     2881 agattggtat tctgtgaatg tgatcacctg tgcagcaccg aatctgcgtc tgctcccggc
     2941 caatgggatg aatcccggag acggtaataa gaccgtgaag atgacgagag cacagcagca
     3001 ggcattgcat gagaagcgtc tgaccagaat tctggatgtg gccgtggcgg gaggcaatga
     3061 agtggtcatc ctgggggcgt tcggatgcgg agcttttgag aatgatccgg aggctgtggc
     3121 agctgcgttt tccaatgtga tcgctcgtta cagaaaatgt tttaaaacca tagaactggc
     3181 catttattgt tcccccaggg atgagcagaa ttataatatt ttcaaacgtg agatagatac
     3241 gacattgcgt tgacaaatga taaggaaact agtactatag ttccaaacaa cacgttggat
     3301 attgagggaa gtaatggaga aggaacaggg aaagcttatt ataattgtat cagtcgtatt
     3361 tgctatggtg cttctgtgca tgatctgcac ctcagacagt gtgccggagg taaagtcatt
     3421 acaggagtgt acgccggacg cagtcagcgt cttggatgac ggcagggagc tgtacgattt
     3481 taaattggat caaaacgatg atgagacgaa ctcgattgtg ttttattcga cacatcagga
     3541 gattgtggtg tatgcagatg ggaagctgat ctatcagctg gatgctgtac ccggcatatg
     3601 gggaaatact ccgggctgga cctggaatat tgtcaggttt tcaagtaatg tgagcagctt
     3661 gcaggtacag ttcacccctt gttatccgga aacggcaggc cagcagaaga cgttttatat
     3721 cgatggcggc tataatatct atcgttgggt gatgcgccgg acaatgcctg cctttctcat
     3781 cagcatgatg gtgatactga taggattgta tattagtatt tattggatcg ttatccgctg
     3841 cggaagccgg atcgacggaa ccttattgta tctgggtatt ttttcgatcc tgctgggaac
     3901 atggtcggcc aatgagacgg atgtggcaac gctgctcctg accaacaggc agggatgctc
     3961 ttatctggca tttgctacgc tcatgctgtt gccgatgtcc tttatcctgt ttgtgaagag
     4021 ctttctggag atcagggatg accggttttg tagaattatc tgtaatgcta atctggcatt
     4081 gatcgtcctg acgcatatac tgaatgcaac agagatatat gagttccgga gaagcctgtg
     4141 gatgacgcat gcgttgatca ttctgatgat tctatacctg ctggtggtca tctgtagcaa
     4201 aattgccaga aggcagctgg atcagaggct gaaggcatgt gtaggggcac tgctgcttgt
     4261 attttttgct accattgtag atgtgagtgg ttattataaa accagcaatg atgtaggtgt
     4321 ctttggcaga atctctttct tgatctttat tgtggtgctg ggaatagaat ccgccagaca
     4381 gacggttgcc cggctgaaaa aaggtcgcag ggtggaggag ctggaacagt ttgccctgaa
     4441 tgattccatg accggcatgt acaatcgaaa tgcttatgat tattttgtga aaaatgagaa
     4501 ggattttgaa gggtatgtca tagtgacctt tgacctgaat aatcttaagc agtgtaacga
     4561 tcaatatggc catagggcag gggatgagta tattatcaac gcggcacata tcatagagga
     4621 caattgtgaa cgctatggga aatgttatcg cattggcgga gatgaattct gctgtatcat
     4681 tccgaaggga agatatttta agatagaacg tgtgatgcat aagatcgatc aggatgtaga
     4741 gatcttgaat cataagaaca tcattcccgt tcctgtggga attgcctatg ggtatgctgt
     4801 attcacggcg gatgatacgg atccggagaa ggtgcgtgag cgtgcagatg aggatatgta
     4861 ccggaataag aaggcgatga agcagtcctg accagagaat ctactcaatt gttaaccata
     4921 ttataaaaag gttgacaatt gaaaaacacc atgctattct aacagaggtt tttacaaaag
     4981 acatctgaca gaaaggcatg gttttttatt atgacaacac agacaacaac tcgtagcaag
     5041 acttatgaca tggtgtacat tgcggtattt gcggttctga tcgcgatctg ctcctggatc
     5101 tccatcccga tgacggttcc ttttacgtta cagaccttcg cagtattcct ggcagtcggc
     5161 gtactgggcg gtaagagagg aagccttgcg gtattgatct atattttact gggtgcggta
     5221 ggcattcccg tatttgccgg tttctccggt ggtatcggtc agttacttgg caacaccggc
     5281 ggttacatta tcggattttt attctccgca ctgctgatgt ggctgatcga gaagctgttc
     5341 ggacgtaaga catgggtgct gggtatctcc atggtactgg gcttgatcgt gtgctatgct
     5401 atcggaacgg tgtggttcat ggttgtatat gcgcagaatt ccggggctgt cggtctggct
     5461 accgtactgg ggtggtgtgt aattcctttc atcattccgg atctggtgaa gatcgcactg
     5521 gcactgacac tgagcaagag actgtctaag gctctgaagc tgaacggaca ggaataaagg
     5581 atacaccgga tatggaagta acaacaactt accggatccg tccgcaccac ggcatgtgcc
     5641 tgtatttttt cgaagggaag ggatacagtg aagggttcac taagcatatg gcggaggtga
     5701 aggaatggct tctggggaaa aacggaccgg catccctgca gctggtcgga gcgacggatg
     5761 agatctgcag cgcctgcccc cataataagg gtggcagctg cgagtctgcg gagaaggtag
     5821 accggtatga tgcgggtgta ttgaaataca ccggattaaa ggcagggcag gaaatgactt
     5881 ttgcggaatt tgagcggatt gtggaagaga agattctgca gccgggatat ggcaaggtca
     5941 tctgtggcga ctgccagtgg agagatattt gccacaaata gcctgaatag gataaaaaag
     6001 cgcacctccc cttaggtgcg cttttttgtg cgtctatagc cacactgtta tctcacgaag
     6061 agataagccg tatatgcaac atacagaagc acgcacgcag ccccctcact gcgggtcatg
     6121 cgtctgccgg tacgggcgaa tacgaagagc agaatggcac tgccaaggag cagtacggtg
     6181 tcgatgacgg actctccgag gacatgcaga gggctgatca ccgcggacat tccgaggata
     6241 aacaggatgt tgaacaggtt ggagccgatg gcattgccca gcgccagccc gctgtctccc
     6301 tttttcgtgg caacgatgga ggtcaccagc tcaggcaggg aggtgccgat ggcgataatg
     6361 gtcagtccga tgaaattctg gctgacacca aaattcgtag ctatgatgca ggctttatct
     6421 accacgagat caccaccgaa gataacggca gccagacctc cggcaatata caaaaggctg
     6481 cggggcaggg agagaatctt gccgggttcg ccctcttctc tgtttttccg ggcagaatag
     6541 atcataaatc cgagatatac gatcatgcca agcagcagga ctgcaccctc aatacggctt
     6601 aagctgccat ctaaaaacat cagacataat acaacggtta ccaggatatt taacggcata
     6661 tcccgcttca gaatctctcc gtggggcata aagctgtgca ggaatgcaca gatacctacc
     6721 accaccagcc cgttgaagat attcgagccg acgacattac tgatggcgat atcattactg
     6781 ccagccaggg cagcattgat gctgacggaa gcctccggag cactggtgcc catggcgacg
     6841 atggtcagcc caatgatgac gctggggacc ttcaggatcc ccgccaggga ggagcttccc
     6901 tccacgaaaa agtctgcacc tttgatcaga agtataaatc ctacgagtaa caatatgtat
     6961 tccataagct gtcctttcct gcggaaagcg tctgtattct tagaaaaaag tataaaaata
     7021 gagactttta ccgcatcaaa aatgatcgat aaaagtctcg ttgcttacaa taataaccgg
     7081 tccgacggac gtgatgacga ttatcattcc ggacagcttc atgtatcatt cggttaccat
     7141 tccaggttac cattctatca gaaaccatcc gggaactact ccctcttaac tgtgccctca
     7201 ttctacaaaa aaggacaagc actgtcaaca gactttgtaa atcttgacac tattttaaca
     7261 cgaactccac cttcacggga tcatggtcgg agtaggaata gccggtgttt acattctcgt
     7321 aggacaggca ctcgatattg tcggagataa tgaatccgtc caaagttacg gtataagtta
     7381 caccggggac atattccata tctgcgtttc tggcactgtt ccagagcgct gcctgctcct
     7441 ctgtacttaa cagatccagg cagaaagcaa aatgctccgg cagttccgtt ctcggaaaag
     7501 gaaatgccca ggactcacag ctctccgtat cctcttcgga agccttcagg tcgtggttaa
     7561 agtcgccgcc gcagaggacg taattgccgg cttcgtattc ttttgccata tcattgcaca
     7621 gcatctcaat ctgtccctgg cggatggcat cgctgttgcc gtaagcggac atatgcagat
     7681 tgaagatcac cagctcgtta ccattatcca ccggaatccg ggagacactg tagcaacggt
     7741 ccagatcgaa gaacttgctg aaggaagtgg agatggggaa gcttcggcgc agagcagaag
     7801 acaccggata tttcgagaac agtccgagcc ctgccctgct gctgccgtgg ggctgtgtaa
     7861 acggatagaa caggaaggcg gaatcataat tctgcgcaaa tacagtagta tattccggca
     7921 gaatgttgcg cagcatctcg tactcatccg tatgatagct tctggtggca tccagatcga
     7981 tctcctggat cagcgcaaaa tccgggtcta atgacttcac cagctctccc gcaccgttta
     8041 tggcatattg tacggtctcc gggctttccg cccaggagga gataccgccg tccatgaaga
     8101 agctgtaatc cggcagataa gcaccgaaac cgatgttgta ggtcactgcg gaatattccg
     8161 tatccgtagc cagcactgca gccgcacctg tggtagtatc gtcgaccggt gcctccacca
     8221 ccagttcctg attgtcctcg atcctgtaat aactggcata gaggtagacg atgtaagcca
     8281 acagtgcgag gatgaggatc cccagcacga tacacatggt ttttaataac gttttacata
     8341 aatttttctt tttcatatcc ttattttaac aagccttgcg ggaaatggca aatgctaaaa
     8401 tttctatgag aaataggcac tcgcatgccg tttttgtatt taatgatgtc ggggtcaaaa
     8461 gcccccgact ttgatgccta cggattgtgg gattatcatc ccacttttat gctcatatca
     8521 gggcgggtca aaaggtcttt cacccactta tgagctagct catgtgggct tagacctttt
     8581 gatcctggca tcatttaata tacgaatggg acttgtacct gtcattatag tgaataatgt
     8641 ttaagtaatg ttgtannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntacga
     8761 atgggacttg tacctgtcat tatagtgaat aatgtttaag taatgttgta ataatgttta
     8821 tataatgtca tatattattt aaaatcctta tcctactgta tcataggcag aaatgcaagt
     8881 aaattttcac ctatgatgta attgtaatgt tgttgtaatg tttagtaatg tttgatatag
     8941 ttatgccaag aacattatta tatggaggag attcatgaaa atcaaagaga tatatccaga
     9001 tgttattaca gaagatacaa gttatgaata taaagcagta ttgaatccgg ataatcccat
     9061 taaatgggca aaaactctta ttggatatgc aaatgataaa ggaggaacct tatttatagg
     9121 tgtatcaaac gatggggagg cctttggaat tgatcttgaa gaagttgata aaacaaaact
     9181 attaatttca aggattaatg atagacatat ttttccacac attaagattt catatatgat
     9241 gagaagtgtg gattctaatg cggaacattt tgtattagct gttaaagttg ctcctgcaga
     9301 ttctgtaata agatatagag aaggagatta caatgaaaca gtttttataa agggagatgg
     9361 aaatgcgact cctgcaacac cggaagaaat tatttcactt tcaaagagaa aatatggtgt
     9421 agataatgag acaagcgagg tggcatatac tgataatcag tggtcagaat accttaatct
     9481 ttgtaaggaa tacagacaag ataaatctgc tccaacaatg aaagaattgc agaacgaaga
     9541 aatagtttca aaagatgggt atgcaaaatc aggatttata atgttttcag acaactataa
     9601 tgaagatgac acaatgatat gctgccgttt atggaaaggg aaaaacaaga caggtattgt
     9661 tttagatagc agcagattaa gaggttctat tgcaaacgta tttgagaatg cattaaactt
     9721 tattgaacga aacaccaaaa caggctgggt aaaaacagaa aagggtggac gggatgaaat
     9781 cagatcgtat cctaaggaag ctgtaagaga ggcattggta aatgccattg cccataggga
     9841 ttattctatt tcgggaacac agattgatgt tgatatctat tcagacagaa ttgaaatagt
     9901 ttcaccaggg tcatggttac tgccaaaaga ttacaatgca tatccagccg gttctatacc
     9961 gtctatcaga agaaatgcca tcatagcagc ttgtctggat gtggcaaatc tgatggagag
    10021 aggtggaact ggttttcaaa ccatgatgga atcttacaag tccagtccag atgaattaca
    10081 gccggtagta tctatatatc ccggattttt gaatttgaga ttatatgaca gattatttag
    10141 agatgaggct atagaactgg atttagaaaa ccttacagat gcggaaaaag ttatggctat
    10201 tcttaagatg gaaggaccaa agcctataaa ggaactacag atgtctctta actatcggag
    10261 tagaagtcaa ttcttaaatg aagttataaa tccgcttatg aaatcagggg agatatatag
    10321 ggatggtaag ccaaaatcac caacggcact tattaaaatc aaaaaataaa agaaaaatat
    10381 aatttgtcag cgaaacaggg ctgcaaatac tgattttgac cggagcgagg ccaacaccgg
    10441 gcatctgatt atatgaaatg tgccagatac ttgccatatc tgatggaaaa ggggtattat
    10501 gataaatata aataataagg aggctgatcc catgaggccg ataccgacga tcggggatcc
    10561 ggagcgctat atagacagtg cgtttgtgga ttccgcgttt atagaattga aaagctgtga
    10621 tctgtgggat gtgaaaatgc tgtatccaat cctacatatg gaccatgcgg aagagaagtg
    10681 cttcgtccgt aaggagatgt atgagaggct ggtgaaggcg gcggggaatc tgccggacgg
    10741 ttaccggttc cgcatctggg atgcctggcg cccctttgca ctgcagcatg aattgtttga
    10801 aaggtattct gtggacatca tccgggattt tcatttagaa gagttgccgg aggcaaaaag
    10861 gagagctgca atctgcaaat ttgtctcgga cccggtaccg gatacaaagg tgcctcccgc
    10921 ccataccacc ggaggagcca ttgatctgac gcttctggat ccggagggca gagagcttcc
    10981 tatgggatgt ggattcgatg catttacgga caagacctgt gcagcgtatt ttgaggcacc
    11041 ggagcatgta cagggggcag aggacgagca ggtccgtgaa aatcgccgtc tgctttatta
    11101 tgctatgata gatgcgggat ttaccaatct gccgtcggaa tggtggcatt atgattacgg
    11161 tgaccggttc tgggcatatt acatgcggaa gcccgcaatc tatgagggcg tatttacggg
    11221 ggaggaaatt catggataat cagaaacaaa agaagaaaaa aagtgtgatg gacaccttgt
    11281 ccgctactat ggtactggtg ctgatcgcac tattgctggt atttgtggga tatacctatc
    11341 tggcactgaa gaacggttac acgctggatg acctgatcga caatatcgtg gggaatctca
    11401 tcggtgtgct ggcggcattt ctgttattcg atatactgaa caacaagctg acccaggatg
    11461 cctacgccag agagacctcc cagcagatca ccaagacgct gatgggagag ccggagatcc
    11521 tggattcttt cagcgatgaa gataaaaaga ctttcctgaa atccaccatt acctccatga
    11581 taaaggatga agatgcggtg gatatgcttt ctaccaatat ggagaaatat tttgacaggg
    11641 caagaggcgc caggatccgg aaaatgttcg attacacgat caatctggag ccgaatctga
    11701 cagaggaata tgtaaaagcc ggattccccg gagccacaga cggaaagtat tacctgatcg
    11761 atgaggaatt gaagttcagc gtaaagtgtc tgggaggaac agatgagaac tacacgggcg
    11821 atgtgatcag actcggtttc gcctatgata aaaagagtct ggacggtgga ttgctggaga
    11881 ccggacagga tgcggatttc accaagagca ttttcaatga ggatctggtg gtggaaccgg
    11941 aagccgtgga cttcatcaac agtattcccg cggataagat acgggatgtg atcaatgatc
    12001 tgttcatgcc gatcttgcga attgacaaca gcggagataa tgacgacgaa gcgcagtttg
    12061 aagtagaacg caagagcaac ggatttatcc tgaaattcaa gttggatttt gaccgcaacg
    12121 aggcaagcgg ggagcatctg gtcagcatct atttcaagat gccccgggta tggaactcta
    12181 ttttcgaggt gactctggtg gatccgacca aggagcccca tatcaagctg aaatacaaga
    12241 atggaatgga tgtcactatg tattcctatc tcaataagga aagcactgcg aatgtcggag
    12301 cctgcatcca gagagcagga ctctatgaca ttgcgatcaa ggacgagtgg atctacccca
    12361 agagcggcgt gatcttccat attaagaagg cgtaggataa atatgaaacc aagatctccc
    12421 caaagtatgc aattatataa aatgctccta tcccgcggtt atccggaagc tttctgtgat
    12481 ctggtgacga agaatctgaa tacggatttt acggcgagcc gtatgatcgg ttatctatgt
    12541 cattattccg atctgccgcc ggaggagatc gcagacgaaa tgctggcgat cttaagtgac
    12601 cgcaatcgca ttatgcagaa gaaagaactg gaaagtaata atgcagagtg gaaccacatt
    12661 ctgatgcagg gattgggcat tgaggaggac gaagatggcg aaatggacga tccggcagat
    12721 ctttgacgga gaatatggct gcgaggagcg gctttccgga gaaggcccga aggtgtcagt
    12781 gacactggtg gatgaggacg ggcttgaaaa atatgtctcc gtggaggata attggcttac
    12841 ggaacaggga ctgaccgagg gttccttgtg gccgggggag ttgacaatat aaagatgaca
    12901 gagaggtact ttaagatgga acacattgca agagaagatt atgtgacggc tggcggtgga
    12961 agataatgca ctgaacatgg agatcgcggg gccgttttac aggatgcaat atgacggtag
    13021 agggagcata caatggaaaa gaggctgtgc agcggagaaa gctgcttttt accgtttttg
    13081 gttgatattg tgaaactttc cgcttataat gaagatttgt aacggcgtaa tttgaatttg
    13141 aggttttcaa tgaatataga agtagacgga tataatatat gttataagat tacaggtacc
    13201 ggcgaccgga ccgtggtgat cttacagggc tggggtacgg atctgggagt gtatgattcc
    13261 gtagccggtg tgatagacgg cagcaggtac cgtgtgatcc agtttgattt cccgggcttc
    13321 ggcggcagcg atgaaccgaa ggaaccctgg gatgtggacg gatttgcgga ttttttctgc
    13381 agatttatgg aagtaatgca ggtaaagcag gccaccctca tcgggcattc ctacggcgga
    13441 cgagtgatca tcaagctggc agccagagag agcatccctt ttgagatcac aaacattatc
    13501 ctgatcgaca gtgccggtgt cctgccggtg cggactactg cacagaaatg gaagatccgc
    13561 aagtacaaga tcctgaagaa attcctgaac atgaagctga tctatgccat gttcccggag
    13621 gtgatcgatg actggaggag ccgacaggga tcggcagact accgcaatgc gacacccatg
    13681 atgcgtcatt gtatggtgaa ggcggtgaat gaggatctga cggagctgct tccgaagatc
    13741 cgtcaggaag tgctgctgat ctggggagat caggatacgg caactcctat ccgggatgca
    13801 catattatgg aagagaagat ccccaactgc ggcctggcag tgatcccggg aaccggtcat
    13861 ttttctttcc ttgagaagcc ggcacagttc cggggaatta tggaggcata tctgaaatga
    13921 tcatacgtgt gctgatagcg gtcgtgcttg ctgttccggc atatttttat accatgcggt
    13981 ataatattca tatgttccag cagaacggct ataagaataa agaacacatt cactggctga
    14041 agaagaagtt ccggctgcaa tggattctct attttggtct ggcactgggc attgtgacct
    14101 gtgtgttccc ataccttgcc cttgaaatct tcgagtatct ggtactgctc gtgatcatcg
    14161 tggtatatcg tgccatgaaa cggatgaata cgaagaaaaa gctggtgtat actgcccggg
    14221 tcaagagatt gctcgctacg gagctgatct ttacggctgc ggtattcgct ctggtgtgtg
    14281 gattgggcgg aatgaagcgg atcccgggac tgtgcctgat cctggtatct gtgaatctgt
    14341 ttctggatat tgtggctaat gtcatcaatc atcccatgga ggcggtgatc aatcagcatt
    14401 atatcaatga cgctgtgaga aagctgaaaa gcgtcccggg acttaccgtc atcggtgtga
    14461 ccggcagcta cggcaagacc agtgtgaagt tctatttgca gacactttta caggaaaaat
    14521 ataatgtact ggtgacgccg gagagtttca atacgcctat gggtgtggtg cggaccatcc
    14581 gcggttccct gaagcctacc acagagattt ttgtctgcga gatgggagcc cgtcatgtgg
    14641 gcgacatcaa ggagatctgc gatatggtgc atcctgagca cggtgtgatc acatccatcg
    14701 gaccgcagca tttagagacc ttttttaata tggagaatat tcagaagacg aagttcgagc
    14761 tggcggatgc cctgccggaa ggcggcatgc tgttcttgaa cggtgacaac gattatattc
    14821 agcagcaggc agcttctccc gcgtatgatc agaccccgga gaagatcttc tattattccg
    14881 agacggaagg gacaggatac tgtgccaaag atgtgaaggt atcccagtta ggtacggagt
    14941 tcaccgtagt gaccccggac ggcgagagcg agagattcca gatgcgtctc atcggtgccc
    15001 acaatgtcat caatgtggtg ggggctatcg cggtcgctca taaaatgggt atgacgctgc
    15061 aggaactgcg gatcccggta cgccggatcg agccggtacc ccatcgtatg cagatgcggg
    15121 agcacggtct ggtgacgatc atcgatgatg cttacaattc caaccctgta ggttccaggg
    15181 cagcggtcga gacactggca atgtttgatg gcctgcggat cctgatcaca ccgggcatgg
    15241 tggaactggg tgacaaggag gcggaataca accgtaaatt cggtggctac gcggcagact
    15301 gttgcgatta catcctgctg gtgggtagac gacacaccga gcccatcagg gagggcgtgt
    15361 tggagaaggg attcccggaa gagaaatgcc tggtattcga caagctggag gaagctgtat
    15421 cttacgccta tgccattaaa gggcagggac ataagtatat tttattagag aatgacctga
    15481 cggataacta ttagaagatt cagaggcaat tttgtgaaag aggttccgga tagttggcag
    15541 agagtaggaa aattcaggag ggaaaataaa tgaagacaag agtagcaatg ctgtttggcg
    15601 gcaagagtgt agaacacgaa gtatcagtga tctccggtat ccaggctgtc atgaacatgg
    15661 atactgacaa atacgaagta atcccggtct atatgaccaa gcgcaatgaa atgtacatcg
    15721 gtgaggagat cggcaagatc gaatcctata aaaatatcga tgaactgttg aaaaagtccc
    15781 agagagtcat catgaccaac gaggacggca aggtattcct gactccgttc ccggtgaaga
    15841 tgttcggcgg aaagaaagcc gtggagatcg atgtggcttt cccggtagtg catggtacca
    15901 acgtagagga cggcgctttc cagggttacc tgaagaccat gggcatcccc ttcgtgggct
    15961 gtgatgtgac cgcatccgcc attggtatgg acaaatacat catgaaagct gtattaaagg
    16021 agtgtgatgt tccggtactg gatgcgcagc tgtatactct gtcggattat gcgcagatag
    16081 agattctgct cgacaaagtg gaaaaaggac tgggatatcc ggtgatcgta aaaccggtca
    16141 acttaggctc cagcgtaggt atcagcgtgg caaagaaccg cgtggaactg acccattccg
    16201 tggacgatgc tttcaagtat gccactaagg tactggtgga gcacgccatc accaatttac
    16261 gtgagatcaa ctgctcggta ctgggagatg agaatgacgc tattgcttcc gagtgcgaag
    16321 aacccctgca caccaaagat atcttaagtt atgaagataa atacgtcagc aatgccaagg
    16381 gcagcggctc caagggcatg gcgagcgtat cccgcaaaat ccccgcagaa ctgtctccgg
    16441 agaagagaga ggaagtccgg gaactggcag tcagatcctt taaagcactg ggctgtaacg
    16501 gtgtgtcccg tatcgatttt atgatcgatg aggacaacgg caagctgtat tttaacgaga
    16561 tcaataccat cccgggatct ctggcatttt atctgtggga gccggtagga gttccttaca
    16621 aagagcttct ggatcgtatg atccagctgt ctttgaagag agcccgcacc gaggagagac
    16681 tgacctttac ctttgacacc aatatcctga attccgcaag ctttggcggt tctaagggtg
    16741 gcaaattgta atagccggtt tatggatttt atgattattt aatatatttt ttagcactcg
    16801 ggacttgcga gtgctaagta gtagtgataa tataatctct gtaagaggaa caagcgactg
    16861 caacagcaga caattgtttg aaaagagatg tgaagtggtc agccgcgcag ggaactgtgt
    16921 cggctgattt ttacatggaa aacgtcttgg ttgtgtggct caccggttgg gcagaatgga
    16981 gggaatatga cgaagacaac aaaaagagat tattatgaag tactgggtgt cagcaagaca
    17041 gcggacgccg cagcgataaa aaaagcatac cggaaactgg cgaaaaaata ccatccggat
    17101 accaatgcag gagaccctgt ggcagaggag aagctgaagg aagtcaacga agcttacgat
    17161 gtgctggggg atgaaaaaaa gaagaaacta tatgatactt acggttttgc tgctttccag
    17221 gagggattta atgaggaggc agccagacag tatcaggagc agtttaagaa cggaggcggc
    17281 ttcggcaccg atggattcgg cggctttggc aacggcacgt tccattacag caccggagga
    17341 agcggtttcg gtaccggtga ttttggcggc ttcggcgggg atgaggatat tttcgacaat
    17401 ctgttcggtc agtatttcaa gggacagaaa ggctccggac ggggcagccg ggcaaactac
    17461 cgtagcaaag gtcaggatgt ggaatccggc atcaccatca gttttgacga agcaatgcat
    17521 ggctgcgaca agactttcac cttaagagat ccggctaccg gcaagtccga atc
//
LOCUS       DAWDHD010000037        17508 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_220_length_17508_cov_10.348765, whole genome shotgun sequence.
ACCESSION   DAWDHD010000037 DAWDHD010000000
VERSION     DAWDHD010000037.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 17508)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 17508)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..17508
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_220_length_17508_cov_10.348765"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         4235 a         4810 c         3480 g         4983 t
ORIGIN      
        1 tgatccggat gccatccaga tccagttcct cgccatcctt cagatacaca tccgcaatca
       61 cagtgaccgg actccccatc gctttcgaca gattgttttt cgtatccaga agaaactttt
      121 tctccgcctc cccggcataa attttcacgg ggttctctcc atgctccccg gcataggcat
      181 ccgctgccgc cttcaattcc tcacagccac cgatatggtc gaaatgtcca tgggtcagaa
      241 ggattgctgc tgtatgtagt ccgtgctgcc caagcgcctc atcaattttc tctccatgat
      301 ccgcggggtc gaataccagc actttatcac tgttctctct gtatacaaag taacagttgg
      361 tctgatagat tcccagcgtc atatgtccga tacggatatt gctcataata atctcacttt
      421 ctgtttcccg acaggttccg ccgttttccg ggcatcacct tatcagcccg tggttctctc
      481 aatatcaata acgctgtcga tccctcggat cttgtcaatg atacggttca gttcctctct
      541 gccgctgatc tcgaaagtcg tctgcagcgt tgccagtccc tgtctgttgg ttctggtgtt
      601 catggacata atatcgatat ttttctccgt cagggccttg gaaatatctg ctaacagacc
      661 gttacggtta ttggcataaa tcttgatctc tgccagatac ttctccgcat gtgcctcctc
      721 cggctgccat tccgcatcga tcagtctcgc cctctccatc tccggcaggt tgatgatgtt
      781 aatacagtcg gtacgatgga tggaaatacc tcttccacgg gtcacatagc ccacaatctc
      841 atctcccggc actggagaac agcacttgga aaaacgcacc gacagatccg cgataccctt
      901 taccacgata ccactcttgg acttcatgag aacaggcttg ttcgcaccat tttgcgcatt
      961 tgccgcattg ttctccgcaa tgcttgccag aacttcctcg ttggtcatct ctttcttatg
     1021 gtcacggtcg tacagctcct gcatcttgtt gacgatctgc ccttctttca gagcaccgtg
     1081 tcctatagcc gccaatacgg aatcccagtc ccggaatcca tatttccgca tcacggcatc
     1141 catgtattcc tgcttctgca ggctggcaag attgatgcct ctggctttgc aataattgga
     1201 aagcagctcc ttgcccttta aaatattgtc ttcctttaat tcgtttttaa accactgatt
     1261 gatcttgttc ttggcctggg tactttttac cacattcagc cagtcacggc tgggaccctt
     1321 ggagttctgc gaagtgatga tctcgatccg gtctccgttg ttgatcttgt aatcaatagt
     1381 caccagtttg ccgttgactc ttgcacccac catcttgttg cccacggcag aatgaatgct
     1441 ataggcgaag tcaataggcg tggatcctgc cggaaggttc ttgacctcac cggtaggcgt
     1501 aaagcagtat acgctgtcgg agaacagatc cagatcgttc tttaacaggt tcatgaattc
     1561 cttattgtcc gacatatccc gctgccattc caggatctga cgcagccata ccagcttctc
     1621 ttcttcctgt acctccggct tcctgccatc ggaagcctcc ttgtatttcc agtgggcggc
     1681 aataccgtat tccgccacac ggtgcatctc gaaggtccgg atctggatct cgaaaggctg
     1741 tccgttgctg ccgatcagtg tcgtgtgcag ggactggtac atgttcgcct tgggcatggc
     1801 gatataatcc ttgaaacgtc ccggaatggg cttatacatc tcatggatca cacccagtgc
     1861 cgcatagcag tccttcaccg tatccacaat gatacgcacg gcaaacaaat catagatctg
     1921 atccaacgtc ttatgctggt tcttcatctt cttgtagata ctgaaaaagt gctttacacg
     1981 gccatcgact ttcgcatcga tccccgcatt tttaatatgt tcgctgactt cgtccacgat
     2041 accctgaatg tatttctcac gaacgctctt gcgcaccgcg atcttatcca ccagatcata
     2101 ataaatatcc ggttccagat attttaagga cagatcatcc agttcgatct tcagtttgct
     2161 gataccaagt ctctgggcga tgggacaata gatctccagc gtctccttgg caatcctctg
     2221 ctggctctcc ggtgtcttgt acttcaacgt gcgcatattg tgcagacgat ccgccagctt
     2281 gatcataatg acgcggatat ccttcgccat ggcgaggaac atcttacgca gattctccgc
     2341 ctgcatctcc agcttggcat ctgactgttc tccgttgctc ccggacagcg gaatcttctc
     2401 cagtttcgtc acgccgtcta ccagaagtgc cacatcatct ccgaattccc ttctgaggtc
     2461 atcctccgtc atcacggtat cttccaccac atcatgcagg atacctgcca cgatggtctc
     2521 cttatccagc ttcagatccg ccagaatgat tgccacattc aatggatgga tgatataagg
     2581 ctctccggac ttccggaact gatttttgtg ggcctcgctg gcaaccttat aggctttctc
     2641 gatcatagag atatcatcac tgggatgata tttttgcaca cattgaataa gctcctgata
     2701 taattcatcc ggattcacga attcttctga tttacttatc ttgtcatcat caaaaatgac
     2761 ttcgttcttc tcttctgtca tagacgaaac agctccatat tatttttatc gatgtcttat
     2821 tttccctcgt agcagatggc tgactccaga cgatatttgc taatgcgctc acggcctttc
     2881 agtcctgcca gttcgatcag cactaccacg ccggctactt caccacccag agactcgatc
     2941 agtttgatca tagcctcggt ggtacctccg gtagcgatca ggtcatccac gatcagaact
     3001 ttctgaccgg gcttgatact gtccttgtgc atctcgatgg tagcagaacc atattccaga
     3061 tcataagtag tggatacagt ctctctggga agcttgccct tcttgcggat caatacaaag
     3121 ggcttatgat tattgtatgc gatgggggtt ccgaagataa atccgcggga ctcgggacct
     3181 actaccacat cataatccag atccttgatc ttctcctgca tggtatcgat cgccaggtgc
     3241 agtccgtccg catcctgcaa tacgcttgtc acatcgcgga aaataatacc tttctccgga
     3301 aaatcgggaa tgctctttac atattcttcc aactttttca tgattctaca cacctttctc
     3361 ttatttattt aacgaattat agctgctttt ctgctacttc aatctgtttt ttaataaaat
     3421 atttgatgat ctcgcttctg gtaataattc cgatgaatac atttctgtcg tcgatcaccg
     3481 gtacaaagtt ctgcatctgc gcctgtccca gcagttcttc catgttggta tcgatacata
     3541 ccggacggat cttgccgctc tggatgatat cccgcacgga cagcttttcc agactcttca
     3601 tggtaatggt atccagttca ctgttgtggc ttaaaatatt ccacagaaaa tctccctcac
     3661 tgactacgcc cacatacctg tcctcggcat cgatcaccgg taccgccgtg tagccgtagc
     3721 gtttcatctt ctccagcccc tgtctgatgg tcatatcgtc ccgaagaaaa gtaacctcct
     3781 ccttcgggga caaaaaagat gcaatattca caatgttcct cctgcctgtt cttttgtgtc
     3841 cattaatttt gatccttagg gtcgacctct tcgaaggtac tgtccaaggc atcctgctcc
     3901 tgtttgaatc tccggagctt gcgcgcaaaa taaatcgtag aaaagcaatc gccggcacca
     3961 ctgaggatca gtaccactcc cagaacacgg aacaacagaa tcgctgcttt gaacggattg
     4021 tagatcagta ccactccgag aatgatgttg attgccgcca caataagaag tcccagccag
     4081 ctgccgtaac ccagtctttt caagtcaatg gcatcctgca gtttgctgca tccgctacac
     4141 agaaccagta ctcccagaat aaagggaatc agggagatga cgatctctac cttgtacagg
     4201 actactgctc ccaacacgat tccgagaata ccgaatacaa actcattgtg ataatagttc
     4261 tctttggcat ctctgagcag atagcagatg atagaaatga gacctgccac gatcagtacg
     4321 ataccaatca gataccccag tgtcttctgc atggtctcgg gaatcaacaa tgccacgatt
     4381 cctaagagaa tatacagcac accggtaaga attgcttccc atttcaattc tttcagcatt
     4441 ttcatatgta acccttcctt tcctgtaact gactgtaact gactataact gtgaaatccc
     4501 gttaaactca ggggaaacga aaaaggggct ctctgcaaac ctgcggaaaa ccccctttct
     4561 gttcaattta ctttctgccg cagcactttt tatacttttt gccgcttccg caaggacagg
     4621 ggtcgttggg ataaaccttc tgctccttca caatagtagt ggaagacttc tgctctttgt
     4681 agagttcctt acgggtctct tcatcgaaaa tagcgttcca ctcttccaga ttgtacagcc
     4741 agtcagcctc tgctgctacc atgttcttgt acagaagagc cttgtcaaag cctaagttta
     4801 cctctgtatc ctcttccaga tcatcaatag ggttcttctc caccagactg tcattaatac
     4861 catccaggaa tccggtcatg gtcatcagag ataccttgaa cttatctgcc agttccttca
     4921 cggtaccctt tactacttca tcgggattct tcagaagctg tgcgtagatc tccttctctt
     4981 cttggaaata agctgcccag agctgctgca gcttctcttt gccggttgcc tcattatatg
     5041 ctactgatct ccactgttcc atcaaattca taattgtaat accatcctta cctaatttta
     5101 ataacacgat ttctgcgccc tcccggatct cgaaatcgtc acatccattc gcatctcgga
     5161 cggaagtcct ccctgctcat gtctgtttat ccgctcaaca gataaactgc ctcgcctgca
     5221 ggcagtcagg ccgttcacct gttggcagaa tgattaatca tagtatataa caacttgtcc
     5281 tcaaaaacaa caaaaaaata tcaacgggaa atgtataatc ccgaaaatcc gggacagact
     5341 actactatca tcggatcccc tcctttgatc ttacggaaga tacgataata cttaatcctc
     5401 cccttttgaa aagaggcacc cacgggtgcc tcttttattg tgttcctgca cctgaattct
     5461 tacaggggaa cttttccttc tttgatcagt ccgataaatg catctgccac cttcggatca
     5521 aactgggttc ctttattttt ctccagttct cccatggcgt attccacgct taaaggttcc
     5581 ttgtatgctc tcttggagat cattgcatca aagctgtcgc ataccgccag gattctgccg
     5641 aggaacggga tctcttctcc cttgattcct ctggggtatc ccttcccatc gtatctctcg
     5701 tgatgggaga caacggccgg gatcacataa ttcatattcg gcagataatg aatcatttcg
     5761 atggacttct ccacgtggct cttcatgatc tcatattctt cgtccgtaag ttttccggcc
     5821 tttttaagaa tgctctccgg aataccgatc ttaccgatat cgtgcagcag tcccgccacc
     5881 ttcacagtct ggatatcgtc ccgggacaat cccagcttct gagccaacag caccgcatac
     5941 tgagagacgt tggcagaatg ctggaaggta aaggaatcct tcgcatcgat ggccgccacc
     6001 agggcataca ccgtaggtgc cacctgctcg taaccggtct ccttatcctg gctctcctca
     6061 ttgataccgc cttcatatac ctggattcgg tccttaccat tacgcttcgc ataaaaggcc
     6121 gcccgcttgg actgctggaa caattccagc gcatccgctg ccagaccggg ataccatgcc
     6181 acaccaacgc tgaatgtaat gggctgcatg acctgtggtt tatttgtagc attctcctgt
     6241 actgcccggg tcagcttctc cgccagctcc accagtggtt ccctgccggt ctccggcacc
     6301 gagaccagaa actcattgga tcctacccgg aatacctctc cgttctccac attatccaaa
     6361 aatctcttcg cgcaccattg cagaatccgg tcgccggtct gttctccgta caggtcattg
     6421 tataacttaa aattatccat gtccatgtat aaaaagcccc gttcttctcc caaggcacca
     6481 tcgcggcgca gatacactcc acaatagctg cggttataca ggccggtcag ttcgtcatga
     6541 atggatacct gatatacctt ttcataagca tcaatgtttt tcagactgcc ggaagcaata
     6601 tttgccatct gccggatgca gtccgcctcg cggtaattga gcttactctc ccgcagacgc
     6661 tccatatata caaatccaca gaccatatta tcatatttta acagggcgat ctccggtgtc
     6721 tcttttgtct cttcctgctc tacgatgcca cagactctct ccacctcatc ttcgcccagc
     6781 gggatatgtc cgctctgtac cacctcatgg taaccgtcat catttatatt cttctcaaag
     6841 attctggatt cccagcccgg aattgcatca tccagaatgt catgcatatt agcaaacaat
     6901 tccttacggt ttaaaatgga tgccgctctg tcctggaatt tccgcagtcc ctccagcata
     6961 tgcttcagct tctggttcag gatatcctcc agacgctttc tggcaaaagc taccaccagc
     7021 accgtccagg cacattgcag aacgatatag atcagcagtt tctgcgttac cgcccgctcc
     7081 agacttccca gcacattttc cagattatga gacaggatga ccaacggcag ggatgccacc
     7141 acaactgcca gaaaatatac tgcacctacc gttacacgat aggaaaaatc aaacagatac
     7201 tgcttgtaca tgatatagac catgcacacc gccataccaa cgccgcccag catatcatac
     7261 ggaaagacgc ttcccggaat caggcagagc agatttccca tcaaaacaaa cagggttccc
     7321 agcaacagcg gctgtagttt tttacgcagc tgtaggtctc caccgatctt gcaatgagcc
     7381 agataagtgg catagaccaa taccgagacc tccaataccg tcaacaccca gattccggat
     7441 gttgcatgat acatatagac ataagttcca tcggcacggg tcatcacttc cggtgccggc
     7501 aggatcgcac cactccaggc attgcccaga accagagctg tcgtcaggac tccatagata
     7561 tagatcagcg catcatgctc cgtaatttcc agaaaagcaa atagaaatgc atacataaac
     7621 actggaatca ccagcagacc aaacagtgca aaattatgcc agaaacgaat tcccggaaat
     7681 acctgtagcc gcatgagaat cgccccaccg gtccacacca ggcaggaagt cagcactgcc
     7741 ctcagactac ggactgtccg gctcttcatg gtattgaaaa agcagaataa cagaaaaagg
     7801 gagcacaaaa aggctcccac cggaacaccg atgtagagat ttctatctaa tgtcatatcc
     7861 agaaagctct ccatacttgt attactccct cacacttttg tttttatttt cgaaccgata
     7921 tccctttttc gacccgaatc ctcttatgta tgtaaaatta taccacattt ttcctttaaa
     7981 gacaatggta cgtccaaaat cctctgattc tctgatccgc gaaaaggcag acgtaaatta
     8041 cggcactctt ccttgtattc tccgtccacg ataacatcca gaagcggtaa aagtttcgtg
     8101 atcaccggat ctcccgcttc catccaggcc agaagatcct tatcaaaatc atagccggta
     8161 tagcaccaga tcgtcttccg gggataaatt tcccttactt tctttaccag agaaagcacc
     8221 gttgcacggt tctgcggttc cataggttcg ccgcccagca agcttaagcc ccggatatag
     8281 tccggggcca gtgcctgtaa aatctcctgt tctgtttcca gcgtgtaagc ctgtccgtac
     8341 tgaaaatccc aggccacact gttaaagcat cccttgcaat gatgggtaca accgctgaca
     8401 aacagcgata ctctcacgcc gggaccgttt gccacgtccg ttttcttgat cgtcgcataa
     8461 ttcataggac atttccttcc cttttactat ctgcatatta cagatgaaga actcttgcct
     8521 tgatctcctt ggtcttgccg gcattccaga agttctctcc aagataaccg caggttcttc
     8581 tggtgacatt cattttcgca tgatccttgt tgtgacactg agggcactcc cattccatat
     8641 tatcattgat catgatctca ccgtcatagc cacagacgtg gcaataatcg gatttggtgt
     8701 tgaactcacc gtactggata ttatcgtaaa taaaacggat cacttcctcg atggcttcca
     8761 cattattcgt cagattaggt acctccacat aagagatcgc gcctcccaaa gatttattct
     8821 ggaattcact ctcgatctta aatttggtaa atgcgtcgat cttctcccgt acatccacat
     8881 gataagaatt tgtatagtat cctttatcgg tgacattgga aatatcaccg tatttttccc
     8941 ggtcgatcct tgcgaaacga taacacagtg tctcagcagg agttccgtag aggctgaatg
     9001 caataccggt ctcctctttc cattttgcgc agcgatcctt catataatcc atgacacgca
     9061 gggcgaattc cttgccgggt tccacggtct ggctgcatcc tttcatcaga tacgtagtct
     9121 catacagtcc gatatatccc agagacatgg tggaataacc attgtgcaag tatttgtcga
     9181 tggtctcgcc cttcttcaga cgggcgatgg ctccgtattg ccagtggatg gggctcacat
     9241 cggataccac accttccagt gccttgtgac ggcacatcaa tgcttcataa cacagttcca
     9301 gacgctcgtc cagcagtttc cagaatttct cttcatttcc ttttgccaga ataccgatct
     9361 gcggaagatt gatgctgaca acgccctgat tgaagcgtcc ttcccacttg tactctccgt
     9421 tttcatcctt ccagggagac agaaaagaac gacagcccat gcaggaaaat acatttccct
     9481 cgtaattctc gcgcatcttc ttcgcggaga tatagtcggg atacattctc ttggcagagc
     9541 actgtgcggc cagtttggtc acatagtcat attttccgcc ctttaagcag ttgttttcat
     9601 ccaatacata gaccagcttc gggaaagcag gagtcacata gacacccttc tcattcttgg
     9661 tcccctcgat ccgctgacgc aggatctcca tgatgatctg cacggtctct tccacatatt
     9721 cgtcattctc atcgatatgc aggaaaagtg tgacaaaagg agactgtccg ttggtggtca
     9781 tcagagtatt gatctgatac tggatggtct gcacaccgga tttcagttcg tcatgcaggc
     9841 gcatctgcac gatcttgtcc ttggctgcct gatccagggt atccccgaat tcttcctcca
     9901 gctgttttgc aaatttttcc ttactcttac gcagatattt ccccagatgc ttgatattga
     9961 cgctctggcc gccgtactgg ttgctggcca ctgccgcaat gatctgggtg gtcacggtac
    10021 atgccacctg aaagctcttg ggagactcga tcatcttgcc gttgatggaa gtgccgttgt
    10081 ccagcatgtc cttgatattg atcagacaac agttgaagat cggctggatg aaataatccg
    10141 catcatggaa atgcagcaca ccgttgtcgt gcgccatggc aatatgctcc ggcaaaagca
    10201 gacgtctggt aacgtcacgg gacacttcgc ccgcaatata gtctctctga gtggaagcca
    10261 gacgggtatt cttgttggaa ttttcctccg ccagctcctt attctcattc cggatcagtt
    10321 ttaaaataga ttcatccgta gtattggact ttctgagcag acttctctgg taacggtata
    10381 caatatactt ctttgccaaa gtatatttat tgtgctctac cagatgctct tcgatcatat
    10441 cctggatatg ctccacggtc tgggtctgtc tgccctcctg ctgtacatcc tccagaatct
    10501 cctcgatcag tccctcactg gcacgatacc gctcctccac ttcattgttg gccttatgga
    10561 ttgcttcccg gatcttctcc tcttcataag gcactactct gccgtctctc ttctgaatct
    10621 tcattgctgt ttccctcact ttatatcatt cccataagat acgaattcct gcataagccg
    10681 aaagaaaagc cgcatcggaa gcaagttccc gacacgactt tttctgcaaa tcatagtgtc
    10741 tcactagatt ttgtgttttc caagtaattt cgtatctaca tcttgtatct aatattatag
    10801 tgaggataca tggaaaaagc aagtatcttt tttaaaacat tcctcttcca ccataaccgc
    10861 cgaagcataa accgccaccg ccacagcaaa ggttgcagaa cagattggcc agacaaattt
    10921 tcaggcacag gtcgttgcca cccacatggg gatagccata ggctgcctgt ctgctctcat
    10981 accaccgtcc cccgctctgt agtctctgca ggagcatctg gtattccata ttctggggat
    11041 ccagcgccac cgccttgttt gcatgttcca gcgccgctac attatttcca aggcccatgt
    11101 gtgccatggc actgtagtaa taccatctgg ccgtcttctc atgctctccc atgccgtcca
    11161 acgtatttct ggcctctctg taatagccgt ttctgagata attaccggct gcccgcagtc
    11221 tgggctcttc ttcatagcca gaagtctgct gttgccagcc tgcctgctgc cagaagtctc
    11281 caaatccctg accgtagccg ccatatccct ggcttccacc gtagccgctt ccgctaccac
    11341 catagctctg cctgccatat ccgccaccgt aactgtagcc ttcggtccgc tccttcatga
    11401 tctgctggta ggctgcctgg atctctttga acttttcttc cgcctgatcc ttattgggat
    11461 tgttgatatt ggcatcggga tggtatttcc ggctcagtgc cttatatgct ttttttattt
    11521 cctcctcgga ggcatctctg ctcactccca gaacactgta tggatcataa gtcatttccc
    11581 gttacctctt ttgcactgtc tttcatccgt ttcttccgca cgatctcata acggcaccat
    11641 acaccggaat acagaatatt ccgcagaata tccacattct gtaaaatagg aagctgttcg
    11701 aactccttac agcactccga catcatcatg gtcaggatct gccggcattc ctcctcaaag
    11761 cccggttcct cataccgttt ctttaacgga ttataggttc cttttttgag atcctgctcg
    11821 atatcctcat aggcatccaa cagataaatg aattttccca ggaaaaatcc aagtctctcc
    11881 agattgtcct tccactcatc ctcacgacag gatacaatct gtgccatgac tctgccaaac
    11941 aatcccgcca tggtatcaat atccgcattg ccctgctttt ccgcatccgc aaaatcctgc
    12001 atggccagtg agatatttct tactttttcc tgatataata acggtttttt acggaatttt
    12061 cttcccaaca gataactgta caccagtccc ttcagactat gctcgtcctc ccagtcgtcc
    12121 tccgctttat agcaggaaaa aagcaccgtc atatctgcca cataatccgt aaacagattt
    12181 cttcttgtgg gatgtttttc caaaggatgg gccacacatc tgcactgggc ctcctgctcc
    12241 tccggttcat ataagccggt gagcagcatc aggataaaag tcatatcgta attcagagaa
    12301 agctgcccca ggacaccgta acgttccttc aggctctgac acaatccgca ataataggat
    12361 cgataaacat catactcctt aaatttcatt tccgactgat tgatcacaat atatccgaac
    12421 atctggctca ccaccctttc ctatatttct gtattccaat tttcatttcc cgtgatcctg
    12481 ccacaggatt ttcccgtcag gtcttgcgga taaattcttc accgcatttt ctgcaccgga
    12541 ttgcgatctt ccccttatgt ctgggtaccc gaatcttctg tccgcatttc gggcatttat
    12601 agatcttgta ctccttacga ccggcaatag cctttttctt tcgctgccac catccgcgaa
    12661 ccttcatttc tttcttcaga taccactggt tctcggcact cctcttactc aggtctttag
    12721 aaaacatccg gtaatacgca tagactaaca gggccagagc cagcacataa aatattctca
    12781 gaccaaaaaa agagagcgcc aggcagacca gagccacaat catcaaaaac tgattgaagc
    12841 ggtcgttgcc ataacgcccc cacatgaaac gctgtaactt ctctttcatg aagtaccacc
    12901 tttcgtccgc caccatacag gggacacatt atccaaatct ttcagagtct ccttcgcgaa
    12961 gtctcctctg aatgttacct acattatggt atgccgaaag gcaaaaagca attaactaag
    13021 tataaacttt tgtaaaagaa ctattattta ttccacagtt tctgggcccg ttcatcccag
    13081 tttttcgcgt attctgcgca ctttccgcac agatgctcca cagactccgg tgactgtaaa
    13141 tcagtggaat gtgctccggt ctcatgcacc atacgctgta agatttccgg attttccagc
    13201 atcgggcagg gctttaagtg attgcagttg aaaggctggt tcgcacggta tgccataaac
    13261 agtggctgct tcaacgcagt cagcagatct acttccttga tattggcacc ggaataatgg
    13321 atgaatacac agggctccac atcgccgttt ggattgatat ggcagtagtt tctgccgcct
    13381 gcgatacatc cgcccacata ttctccatcg ttctggaaat cgaaggcaaa gataggtttt
    13441 ccgcccttca tggcacggat ttcacgaata cggtgataca tatattctct ctggtccgga
    13501 tctaagagca attcggtaga tgcatcattg cctacgggca tataatggaa ataccagctg
    13561 aatctggcac ctttttcaat gatcatatcc agaaattcat cactggtgac tactttatag
    13621 ttcttgctgg tatagcagat ggaagttccg aagatcagac cgtactgctt catcagatcc
    13681 atggcatgca ttactttatc aaacacgccc ttgccgcggc gtaagtcgtt cacttcgtcg
    13741 aagccttcca gactgatagc tacggagaag ttgcctaccc gcttcatctc ctggcagaat
    13801 ttttcatcga tcagcgtgcc gttggtaaat gcatggaagc cacagtcatc gtgtttctcg
    13861 cacagacgga tgatgtcatc ctttctgacc agaggttcgc caccggtgaa gaaatacaga
    13921 tagataccaa gttccttacc ctgggtaacg atgcgatcca tatcttcaaa gcttaagttc
    13981 agacgattgc cgtactctgc cgcccagcag ccggtacaat gcaggttgca ggcactggtg
    14041 ggatccatca ggatcagcca ggggatattg cactgatgtt tcacacgcat ctcgttgatc
    14101 tcttttttac cgcagaacgc cgcctcaaaa ccatagtcca gtgcggacat tttgatgaca
    14161 tggggatcca cttcgtttaa gatccggttc acatactgca tccatttgct gtccggatcc
    14221 tggatcatac gtcttgccgc atcatagctt tccttgcgga aggtatctcc cataaatttt
    14281 tccgacagat ctaccagctt taagaatgcc ttttctctgt ctgtttcgtc cttattcaca
    14341 tacttcagaa ttccgtcgat tgctgcgcca aatgcagctc tctcaacttt gtgttttgcg
    14401 tctgccatac gatttacctc acttttgtct atttattcta tgaatcacgg tatttcagat
    14461 gtaatatcga aacaatacat ccacgccggc aagcaggaag atcagcgctc cactgatcag
    14521 tacacggttt cttccatgac acccgatccg atatcccgca tagatgccga cgatgaccgc
    14581 cagcgcagcc ataattgcca gctgaatgat ctgcaccgtt actctggtgt ctaaaaagcc
    14641 catgccaatt cccgtcagaa aactgtccac actggtaagc atggcccaaa tgcatagctg
    14701 tctccaggcg aaggcatctt cccttcgttc cagcgggtcc cgcatatgta gtgcccggac
    14761 gatcagccat atcccgatca ggaaaaaaat aactgccgat gccgccctga acagtctttc
    14821 tgtccgctga tagttcattg caatgtacgg aagttccgaa atcaggcttc ctaaaagcat
    14881 tgcaccgctc tgccatcctg tgaacagcag gatcattttc aggatctgct ttttgtccag
    14941 tctggaaagc atagcgcctt tgtataatgc ataggtaaag acatccaggg atagtcccac
    15001 cgatacaatg aaaatttcat accatgccat ccatgcaacc cctttctccc gttataatgt
    15061 ttttatctct tttcattttt tactttaaac aattttccgg catatggtaa gatacaaccc
    15121 ttgcacagcg tttttcctgc tcttacattt tttcgataac tgtaaatatt ccgcgacatt
    15181 ttggcaaaag tgtcgtttaa agttgagaaa agaaagtttt ttggttataa tatttctatc
    15241 ccggttcttt tataggaaaa ggagtcctgt gatgaagaca aaagaattat tggcagacag
    15301 ttttcgttct cttgtgctta ccatgccttt tcagaagatt tccatcaaaa tgatcacgga
    15361 tggtgcacat gtgatccgcc ctactttcta taactatttt caggataaat acgaagtgat
    15421 tgagtttctg ttcgaccagg acatcggcag caaagtcgaa gtcatgatcg aaaacgatat
    15481 ggaacaggaa gccatcaagc tgatgtttat ctgttttgaa aaaaataagg aatattatca
    15541 tcgacttttt gaaaccacag gccagaattg cttcggagaa tttttttcac actactgtga
    15601 acagaccttt ttccggattc tgtccagaca tcctatgagg aaaatgccct gcgaacttat
    15661 cacaccgcag tcactggcac attataatgc cctcttatta attgaaattc taaagttatg
    15721 gctctcctct ccgaaagaag tccctgcgga gaaaattttt gaggcctacc aatatattat
    15781 acgaactccg atactggata tgatccaata tccggaacca taaaaagaca ggtcgaactg
    15841 acctgtcttt ttatatctga taaacttata atcttgtgat cacaaagata tcatagattg
    15901 cctgaattgc agtctcaaaa tcttcatttg ccacaccgat gatgatgttc agctcggaag
    15961 atccctggtc gatcatcttg acattgatat ttttgtgtgc cagtgcggag aagattctgc
    16021 ctgcagtgcc gcgggtggac ttcatgccac ggcctacgac agcgatcaat gccaggtcgg
    16081 attcgatgtc aatggcgtcg ggattggcca gtctatggat gcctgccact accttctgtt
    16141 ccttgtgcat gaactcatcc tgatgtacga ataccgtcat ggtatcaatg ccggagggaa
    16201 catgctcgaa agaaatacca ttttcttcaa atgcctgcaa tacctttctg ccgaaaccga
    16261 tctcggagtt catcatatct ttttcaatat tgacggagca gaagcccttc ttgccggcga
    16321 taccggtgat cacgtatttg gacttctgac aggtgcttcc cacgatccag gtaccattgt
    16381 cctcgggagc gtttgtattg cggatattga tagggatacc ttcctgacgt accgggaaaa
    16441 tggcatcctc atgaagaacg gtagcaccca tataggccag ctcgcgaagt tcacgatagg
    16501 tgatagtctc aatagcctcg ggatgatcaa tgatgcgggg atccgcaatc aggaaaccgg
    16561 atacatcggt ccagttctca tagacatccg ctttggcagc cttggccacg atggaaccgg
    16621 tgatgtcgga accgcctctg gagaaagtct tcaccgttcc gtcgggcatg gcaccgtaga
    16681 agccgggtac aactgcttcc ttgctctctg ccagtctggc acgcagtact tcattggtct
    16741 ttgctgcgtc aaattcgccg ttctcatcga agaagatgac ggttgccgca tcaatgaaat
    16801 catagcccag ataatttgcc atgataatac cgttcagata ttcaccacgg gaagccgcgt
    16861 aattgctgcc ggcctttgcc ttaaaattct tctcaatagt cttgaactca tcgacaagat
    16921 ccagatccag ctgcagtccg tcgatgatct cctgataacg ggctttgata gcatccagct
    16981 ctgccttgaa gctcttgccc tgatctgcca gatcatagca ggcatacaac atatccgtta
    17041 ccttggtgtc atcggaaaat cttttccccg gtgcggaagg taccacatat tttctctcct
    17101 tatctgcccg gatgatgttt cctacttttt tgaactgctc tgcgctcgcc agagaacttc
    17161 caccgaattt cactactttt gacataacat tttctcctca actctatcta ataatagtat
    17221 atccttttta taccagacgg atcctgccga gcatgtgctc gcccagtgcc tcgtattttg
    17281 cctgaaaatc tttttccttc atactctgtg tgaagtatcc gaagtcttcc ttccggtcct
    17341 taagatgtac catccggcct ccggggaacg ctgctgtaac cgcatcttcc gctccggcct
    17401 ttgcccggat gaagaaactt ctctcggcct cgccgatgtc tgccagcttt acttcctctt
    17461 tgtcccagaa gcacatgatc accttgccct gatgtcttgc acagtcga
//
LOCUS       DAWDHD010000038        17335 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_222_length_17335_cov_12.779051, whole genome shotgun sequence.
ACCESSION   DAWDHD010000038 DAWDHD010000000
VERSION     DAWDHD010000038.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 17335)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 17335)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..17335
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_222_length_17335_cov_12.779051"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         5034 a         3632 c         4681 g         3988 t
ORIGIN      
        1 cggccgaccc cagccgggtg aaagcggcag aggcgcagaa cccctgggaa gagtaccgga
       61 cggcagcgga atccgcaaaa ctgtccgtgg aactggcggc caaagcggca gaggaagcca
      121 gagaactgtt cgccagagag cttagggtct ctgtgatcat tccttccaaa gataatcctt
      181 ccgtactggg aaaatgtctg cggtcgctga cgcaacgtcc ggagggcagt gttccggtgg
      241 aaatcctgct gatagataac ggaagtaacg aggaaaacag gaaaaaaaca gaacagctgg
      301 tggaagaaat ccggacagcc ggaacaccga tccggtatgt atatgagcct gcggagttca
      361 atttttccac catgtgtaat cgtggcgcag aactggcaga tggcaagctg ctgctgttct
      421 taaatgatga tattgaatta tgtgagaatg actggctgga taaaatggtg tccagagcct
      481 tgcagcctta tgtgggcagt gtgggactga aactttatta tccggattcc gtgaagatcc
      541 agcatgacgg cattgtcaat ctgccggtgg gaccggtgca taagctacag tttatggagg
      601 atgacagaag ctactatttt ggacggaacc gttttacaca ggattgtgtg gccgtgacgg
      661 gagcctgtct cctgatccgg acagaggtgt tccgggaggc cggaggattc cgggaagtgt
      721 tgcgggtagc ctataatgat gtggaactgg gctttcggct tctggaaatg ggatactaca
      781 atgtggtgtg gaacgaccgc tttgcatatc accatgaatc tctcagccgg ggaagcgatg
      841 aaagtcctga gaaaatgcag cgtctggtac aggagagaga actgctctac cagatgcatc
      901 cacagttccg gggagaggat cctttttacc cgaaggggct gaaccgggag ggactggaca
      961 gccgtgtggt tccggcatat ctgacagatc ggaatgtttt acaggagcct ttctggaaaa
     1021 ggggacttcc tggcggagag gaattacaga agatccgcag ggataactgc ctgatggcca
     1081 gagtagagac agcgggaccg gagcggatcc agggatacag tgtaattctg ggagacgata
     1141 atgcctgcta tgaaaaacat ctgttgctga ttccctgtgg ggaaacagaa ggacaggatg
     1201 tgtggtccat gcagttgatg cctgcctacc gacaggaact ggaggaaaat cttccggatc
     1261 agaaaaatgt ggcattgggc ggcttttgtg tcctcagaga aggagaacaa ctgccggcgg
     1321 gaaactatac gatcgctgtt cttgtggtga atcgtgtcag caagctgaaa ctttggaata
     1381 ctacgggaaa atatctgacc gtagagcttc cggcagcaaa agaataaaac agatgcatga
     1441 tccgctaaaa tgcggtgaaa gcatcacgga ggagacatat gtcagagagc aacaccgaga
     1501 tcaattataa ggaagcctac gagagagagc atgaaaaatg tgcggatctg gcggacaaga
     1561 tcgtagtact ggaatcggag aaggaagatc tgcagttcaa actggaccga atcaagaaaa
     1621 ataaattctg gaaggctaca ggacctgcca gaagtgtgat ccattttgcc cagagacaga
     1681 aggaacggct gtctcattgc ggtggtccca aagatattct gcggaagatc cgtaacaaga
     1741 gtcaccagaa gagcctgatg gaatcctacg gtaccggaag ctttcccgat gcggagcaga
     1801 ggaagaaaga ggaagaaacg gtattcccct atatgccgaa gatcagtatt ctcgtgccgt
     1861 tgtggaataa caagagagag ttccagatac agatgctgga ctgcgtcatg aaccagacct
     1921 atcagaactg ggaactgtgt ctggcggacg gttccgatgc ggaacatgct tacatcgaag
     1981 aactctgcaa ggagtacgcg gcgaaatcag atggccgtat tgtatataag cacctggacc
     2041 ataacggagg tatttcctac aacaccaatg agtgttacaa gctggccacc ggagactata
     2101 tcggactgtt cgatcaggat gacattctgc atccttccgt attgtatgcc tatgtcaagg
     2161 aaatcaatga acagggtgcg gattatctgt actgtgatga gactaccttc caggaggaca
     2221 atattaataa aatgctgacc atgcatttta agccggatta tgcaccggat aatctgcggg
     2281 ccaacaatta tatctgtcat ttcagcgtct tttccagaaa acttctggaa ggcgaggaac
     2341 tgttccgtac ggcttttgac ggtgcgcagg accacgatat gatcctgcgt ctgacggaca
     2401 gagcacaaaa gatcgtacat gtgcccagac tgatgtatta ctggagaagc catgcgggca
     2461 gtaccgccgc cagtattgac gcaaagccct atgccatcga ggcggcaaag ggtgccgtgg
     2521 cggatcatct gaaaaagcat ggattcaaac attttcagat caccagtacc agagcctgtg
     2581 ccacaatttt ccggatccgc tatcagattc tgggagatcc gaagatctcc atcgtgatcg
     2641 ccaacaagga tcaggtggaa gatctgaaga gatgtattac ttctattcag aagaacagca
     2701 cctggagcaa ctacgagatc atcgtggtgg agaacaacag caccacaccg gaaatccggg
     2761 attactattc ccagctgctg ggattgtccg gcaatgattc ctacgcagag agatgcaagc
     2821 tgcataccgt ctgcggtcat gacggcggga ttctgcacag cgaggacggc cggatctctg
     2881 tggtgaccta tcatggtgac tttaattatt ctgctgtaaa taatctggga gtatcctatg
     2941 ccaccggaga ttatatcctt ttgctgaaca atgatacgga agtgatcact gccaactgga
     3001 tggaagagat gctgatgtat gcacagcggg aagatgtggg tgccgtaggt gcaaagctgt
     3061 attatgcgga caagacgatc cagcatgccg gtgtagtcat tggactgggg gcacatcgca
     3121 ccgcaggaca tacccattat cgtattccgg tacagaacct cggctacatg ggacgcctgt
     3181 gttataccca gaatgccacg gcagtgaccg gagcatgtct gttggtgaaa aagagtctgt
     3241 atgagcaggt gggaggactg gacgagagct ttgtgatctc tctgaatgac gtggatttct
     3301 gcctgaaact gcgcaaactg ggactgctga atgtatggac cccttttgcg gaactgtatc
     3361 attatgaatc tatttccaga ggtctggatg atcagggaga gaaggcggag cgttacaaca
     3421 aagaatccga gcatttccgt gaaaaatgga aggcagagct ggaagcagga gacccctatt
     3481 acaacccgaa tttctctctg gatcgcagtg actatgcgct gagagatccg gttagcggac
     3541 gttaaattgc tcttttcagt aaaggtaatc tatggtagaa taatggcgga gagcgtttac
     3601 tgaagaaaga taaagatagc gataaaacag gaggatagga aaatgagtta tatggacgaa
     3661 ctgaatttct ggttgaatga tgattatttt gaccagaaga ccaaggatga acttctggca
     3721 atccgcaaca acgaggcaga agtggaagat cgtttttaca aggaactgga attcggtacc
     3781 ggcggtttac gtggcgtgat cggtgcagga accaaccgca tgaacgtata taccgttcgt
     3841 aaggcaactc agggtcttgc taattatatc attaagcgcg gcacccagag caagggtgtg
     3901 gctatcgcat ttgactcccg ccgcatgtct cccgagtttt ccgatgaggc agcaagatgt
     3961 ctggcagcca atggcatcaa ggcatacatt tttgaatctt taagacctac cccggaactg
     4021 tcctttgccc ttcgtacgct gggatgtacc gcaggtatcg tagtgactgc aagccacaac
     4081 cctcctgagt acaacggcta caaagtatac tgggaggacg gcgcacaggt aaccgctcct
     4141 atcgataagg acatcatcac cgaagtacag gccatcaagg attatcatac cgtgaagacc
     4201 atgagcagag aagatgcgga aaaagcaggc ctctatcagg tgatcggtaa agagatcgat
     4261 gacaagtaca tggctgagct gaagaagcag atcattcatc ccgatgttat tcaggaaatg
     4321 gctgatgaca tcaagatcgt atatactccg ttatgcggta ccggtaacaa gccggtaaga
     4381 cgtatcctgt ccgagctggg atttaagcac gtctatgtag ttcccgaaca ggagaatccg
     4441 gatccaaact tcaccacact ggattacccg aaccctgagg atcccaaggc atttacatac
     4501 gcactgcgtc tggccaagga gaaggatgcg gacatcgttc tggcaaccga tcccgatgca
     4561 gaccgtctgg gtgtgtatgc caaggatacc aagaccggcg agtatgtggc atttaccggt
     4621 aacatgtccg gtatgctgat cgcagaatat atcctgagag agaagactgc taccggcgct
     4681 atgcccgaga atccagcact ggtaaccacc atcgttacca ccaatatgac caagcccatc
     4741 accgaagctt atggtgtaaa gctgatcgaa gtactgaccg gcttcaagtt catcggtgag
     4801 cagatcaagt tcttcgagca gaccggcagc aacaattatg tattcggtct ggaagagagc
     4861 tacggctgtc tggcaggtac tcatgcaaga gataaggatg cagttgtagc agttatgtgt
     4921 ctgtgtgaag tggcggctta ctgcaagaag cacaacatga ctctgtggga tatgatggta
     4981 tccatgtatg agaaatacgg ttacttcaag gagacccagt acaccattac catgaagggt
     5041 atcgatggtg caagacagat cgccgagatc atggataagc ttcgtaagaa tccgcccaag
     5101 gcattcggtg acctgaaagt ggagaaattc cgtgactatc tgaacgatgt agtcatcgac
     5161 atggagagcg gcgagaagac tactaccgga cttcccaagt ctaatgtcct gtattttgaa
     5221 cttcccgaca atgcatggtg ctgcgcccgt ccttccggaa ccgagcccaa gatcaagttc
     5281 tacatgggtg ttaaaggtaa cagcatagag gatgcacagg ccaaggtaga gaagctgacc
     5341 gaggatgtaa aggctgtact gtaaccacca tgagcaaata gcatgcaagt atgttattca
     5401 ggaaagctct ggaagagcga ttttgcgaat ggggttctga atagttaatg ttatttttaa
     5461 gagcgaatcg cccgggaagg gcgatttgct cttttataaa agtggaggta tccgtggcaa
     5521 agctgaacat agcaaacctt aagaaaactc tatattattt gcaacgcaac ggcctgaggg
     5581 aaacctggat ctctgtaagg gaacgcctga cagagacaga ccgctatttt tatgtgccct
     5641 gtccggaaga ggaactggaa aggcaaagtt gcagaaaatg ggataatccg gttacactca
     5701 gcattgtggt gccgttgtac cgtaccccgg aaatctatct gaacagaatg ataacaagcg
     5761 ttatgcaaca gagctatcct cactgggaac tgattctggc ggatgccaca gaggatcaca
     5821 gtgtggagga gacgttgaca aatcagggat tcctgacaga acggctcctg gaaaatgcgg
     5881 aaactattgc agcggatgca aggatccact atatacacct tacggaaaat gccggaattg
     5941 cggcgaatac caatcaggca ttaccctatg caaggggaga atatatcggc ctgctggatc
     6001 atgatgatgt attgacaccg gatgctctgt atgagatggc agatgctatt accaaagcaa
     6061 atgacagagg tgtcagagtc gcattcgctt attcggatga agacaaatgc aacggggatg
     6121 agacaaaata ttacgatccc aaccataaag aagactttaa ttacgattta atcctgagta
     6181 ataactatat ctgccatttt ctggtgatgg atgcagatct gatgaagaaa ctggccttcc
     6241 ggccagaatg cgacggtgcc caggactatg atctggtgct gcgggcagtg tcagaggtgc
     6301 tggctgcaga tggccgctcc ggggaggaga gaattcttca tattcccaga gtgctgtatc
     6361 actggagatg ccatgaagca tctacagcag ccaatcccca cagcaagaaa tatgcttacg
     6421 aagcggggct gcgtgcactg caggatcatg cggcagaacg tgggatcccg gcaaaggcag
     6481 aggagaccag acatgtagga ttttatcggt tacaatatgc agaggtatta caggagagac
     6541 cggatgtggc tgccgtaggt ggcagagtgc tgtctgggaa aaaccgcggc aggatcgccg
     6601 gcggcagaat gactgcggac ggaaaggtgt tctacgaggg actgccgaag gactttggcg
     6661 gttatcttca ccgggcggag ctgtcccagg atgcagaagc gttagacctt cgctgcatca
     6721 ggatccggtc agctgacagg gaattgtttg aaaaaatcgt gggagtgcct tatacggaag
     6781 tagtaagagg tccggaacaa cagccggtat ttgacagctc cacactgcct gccggggcag
     6841 atatccggct tttaagtctg caattatcgg aagcattacg aaaacgggga agactgctgt
     6901 atctgccgga gtatccggag aaatgggaga gactatgaag gaagtaaccg tcgttatacc
     6961 caattacaac ggaatgaaat atttaccgga atgcatggct tccctgtgtc agggacagga
     7021 aaatgcaccg gaatatgaag tgctgatcat agacaatggt tctgctgacg ggagcgtgga
     7081 atatctggac agcgaatggt gtcacaaaaa agaggcaccg gcagaagagg ggggaaaacc
     7141 gcaggtgcgt ctgatcgccc tgccggagaa cactggcttc tgtcatgcgg tgaatctggg
     7201 aatccgggag gcacgaagcc cctatgtgat cctgttgaac aacgatacca gagtagaagc
     7261 tggatttgtg aaaggattgt atgatgccat tcggaaggat gaaaagatct tttccgtcag
     7321 cgccaaaatg cttatgtggg ataagccaga gctgctggat gatgcgggag accgctactg
     7381 cgttctggga tgggcatatt cccggggaaa aggccggcct gctgtggatt atgacaagaa
     7441 tgtcagcata ttctcagcct gtggcggagc ggcaatttac agacgtcagg tatttgagga
     7501 gatcggctat tttgacgaag cgcattttgc ttatctggaa gatctggata tcggttaccg
     7561 tgcccggatc tacggctatg agaaccggta cgagccgacg gcatccgttc tgcattacgg
     7621 cagtgcttct acgggatccc gctacaattc ccggaaaacg gaactttctt cttccaataa
     7681 tatctatgtc atcggcaaaa atatgccgct actacagtgg atcctgaatc tgccgttctt
     7741 tttgcttgga tttggcataa aatttctgtt tttttgtaag aagagaatgg gaaaactata
     7801 tttaaaggga ttggccgaag ggtggaaacg taccgtgggt cctgtgggaa agcaggcaaa
     7861 agtgcgcttt caatggagtc atctatggaa ttatgtgaag attcagtggc aattatacct
     7921 gaatgttttt cgaattctta tgaaatctta agaaatcgct tcataaattc ttaagttgag
     7981 aaaaaaccac cgttattgta gaatgaaatc gttgaaaaga agcttttcgg acttaaagat
     8041 tgggtgaccc gtatgataaa agacaaccag aaagtcttta acaggcttct tgtgataata
     8101 gatgcgctga tcacggcagt atcttttgtc cttgcctatt atatcaaatt ttatatctta
     8161 aatgacggtc cggggatcgg tgtcctgccg gtgcaggatt attatatgct gcttcccttt
     8221 attgtacccg gttatatgtt cctgtattac cggtgcagtg tgtattcccc taagcgtacg
     8281 gttcgccgca gacatgagat atacagtatc ctgcaggcga ataccattgg cctggcggct
     8341 ttgatcatta ttttgtatat gatcatccgt gagatcaatt tttcacgatc cgtcatggca
     8401 attttttatg tcctgaatgt ttttctgact tccgtattcc gtatcatcat gcgcaaggca
     8461 ttgcgttcca tacggaaaaa aggatacaat ctgaagcata ttttgctggt aggttattcc
     8521 cgggcggcgg aggaatatat cgacagaatc ctcagcaatc cccaatgggg atatgtagtg
     8581 tgcggtattc tggatgagca tatccccggg ggcacgacct ataagggagt gaaagtactg
     8641 ggaacgcttg gtaatctgga atacatcctt ccggagaaca agctggatga aatcgcgatc
     8701 acactttccc tgaaggatta tgattatctg gaaggtgtgg tggatatctg tgagaaatcc
     8761 ggggtgcaca ccaaattcat tccggattac agcagtctga tccccagcag accctatacg
     8821 gaggatctga tgggattacc ggtgatcaat atccggtatg taccgctgac gaacacaggc
     8881 aatatggtga tcaagcgggc aatggatatc gttggttcca ttttcggtat cattattacc
     8941 tcaccgatca tgctcatatc agcaattctg gtaaagctgt ccagcccggg accggtgatc
     9001 tttaagcagg aacgggtagg acttcataat aagccgtttt atatgtacaa attccgcagc
     9061 atggcaatgc agacggcggc ggaagagaaa aagggatgga cggtacgcaa tgaccccaga
     9121 gtcaccggta tcggtaaggt tctcagacgt accagtatcg atgaacttcc ccagctgttc
     9181 aatatcttaa aaggagatat gagtctggtc ggtcccagac cggaaagacc gcagtttgtg
     9241 gaaaagttca aggaagagat tccccgttac atggtaaaac atcaggtacg gccgggactg
     9301 accggatggg cacaggtgaa tggcttgcgc ggtgattcct ccatcaagaa gagaatcgaa
     9361 tatgatattt actatattga gaactggacc attggatttg atatcaagat cattcttatg
     9421 acgttcttta caggatttat caataaaaat gcttattagt acaaggagaa gacaagatgg
     9481 caacaaattc taacaacaat ggcggcaatg gtcagagacc tgccggaagc ggaagaaaac
     9541 cttccggaag cgcacagaga cctacaggac agagacccgc cggaagcggt cagagacctg
     9601 ccggcagtgg acagagaacc ggagctgcga acggacagaa gagaatgtct gccaaacagc
     9661 gtgcagccaa gaagagaaga aaggttatca tctttggcgt agagatcgta gtgatcctgt
     9721 tcatggtagc agtattgtat ctggtaatga ctcataccag caatgagggc cccaaggtaa
     9781 ccgttctgga gacagaagaa cttcagattc ccgaagaggt gcagcaggat gaaactatga
     9841 agggctatat gaacattgcc ctgttcggtg tggatgccac caaggaaagc ctgttgtaca
     9901 agggcagccg aagcgatagt atcatgatcg ccagcatcaa catggatacc ggtgatatca
     9961 aactggtcag cgtatatcgt gatacgtacc tgaatatcgg tacggattcc taccagaaat
    10021 gcaatgccgc ttattcctat ggcggagcag agcaggcggt taagatgctg aacatgaatc
    10081 tggatatgga tattacgaac tttgtgaccg taggctataa aggactgagc gaagtgatcg
    10141 acggtctggg cggtatctat attgatgtgg actctgagga gatcaagcat atcaacaact
    10201 atcagatcgg tattgcagaa gttttgaaat gcgattatac accggtaact gagaccggta
    10261 tgcagctgtt gaatggtctg caggctactg cttactgccg tatccgttat accgctggta
    10321 acgactttaa gcgtgctgcc cgtcagcgtg aagtcattca ggcaatcgaa gatcaggcaa
    10381 agaaagcaga ttatgcaaca ttgtccaagg tattcaacag tgccatcgac gacatttaca
    10441 ccagtctgga ttccaaggac atcctggatc tgctgagcaa tatttcaaat tatcgcatag
    10501 tggacgaagg aggtttcccc gaagagacca tgcgtgacac cggcaatatc ggtgcaaagg
    10561 gaagctgtgt tattccggta aatctcgaaa gcaatgtagt atggctgcat cagttcctgt
    10621 ttgatgatgc caactattcc gtaaccagca acgtgaagga atacagcaat aagattgaat
    10681 ctgacacatc accttatatg aataagtaag aaacagtgta tagagtaaag caacaggaat
    10741 acaagggagt cttccggaat ctgccggggc tcccttgtgc attacccaga aggttataat
    10801 cctcttggac agtaggaaaa ggcttgtgcc gcaataagag ttaaagtata atgaaccaga
    10861 gattggaaaa gtgaggagcg atctgacatg gagatagatg tgatcattcc cctgtataag
    10921 ccgggaaaag aattatttac attattggat aaactgggca gtcagagcgt accggtacat
    10981 caggtgattc ttctgaatac ggaagaaaaa tattttgatc agctgatcta cggaatcaga
    11041 tttctggaaa cctatcagaa tatcaaggta taccatgtat ccaaaagaga gttcgatcat
    11101 ggcggtacga gacggatggg agtaaaaaag tcttctgcgg atgtcttcgt gatgatgaca
    11161 caggatgcca tgccgaaaga tgacaggctg atcgaaaaac tggtggaacc cctgcagggg
    11221 gaagtggcgg tggcctatgc gagacagctg cccagagagg attctacacc ggtggagagc
    11281 tataccagag aatttaacta tccggcaaag tcccggatca aatcggcagc agacctggac
    11341 agcctgggga tcaagacctt tttctgctcc aatgtctgtg cggcctatcg ccgggagatc
    11401 tatgaggaac tgggcgggtt cgtcagacat acgatattta atgaagatat gatctatgcg
    11461 gcaaaggcgg tggaagcagg atactctgtg gcttatgcgg cggatgcgca ggtggtgcat
    11521 tctcataatt ataccaacgg gcagcagttc cacagaaact ttgacctggg agtatcgcag
    11581 gcagagcatc ccgagatttt tgcagcatat cattcggagt ccgagggaaa acgaatggtg
    11641 aaagatgtga cggtatattt gcgcagcaac catatgtctg ccggattgcc ccatttttat
    11701 atgcaatgcg cctgtaaata tgcaggttat ttactgggaa agaactatcg caggctcccg
    11761 aaaaaatggg tacttgcttt tacttccaac aaggaatact ggaaacagaa cagaaaggat
    11821 gtatgactat gtgtggtatt gtaggttata ttggaacaga acaggcagca ccgatcattc
    11881 tggacggtct gtccaagctg gaataccgtg gatatgattc cgcaggtatg gcgatttttg
    11941 acggtgagaa gatcaacacc agaaaggcag tgggaagact taaggttctg gagaacctga
    12001 cccacggcgg agagaatatg aaaggaacct ccggtatcgg acatacgcgt tgggcaactc
    12061 acggagctcc cagcgatatc aattcccatc cgcatatgaa caatgcggga accattgccg
    12121 tggtacacaa cggcattatc gagaactata ttcccctgaa gaaaaagatg caggataagg
    12181 ggtatcagtt cgtatccgag actgataccg aggtattggc acatctgtta gactattatt
    12241 acaagggaaa tcctctggaa gccatcacga aggtgcttca tcgtgtggaa ggctcttatg
    12301 cgctgggaat catgtttgca gatcagccgg ataagatttt tgcagcccgc aaggacagcc
    12361 cgctgatcgt aggaaagagc gacaacggca gctttatcgc ttccgatgtt cctgcgatat
    12421 tgaaatatac cagaaccgta tattatgtag acaatcagga agttgtggaa ttacagcagg
    12481 gtagtctgcg tttcttctcc gtggatgagg aagagatcga gaagcagccg gtgaccattg
    12541 actgggatgc caatgcggca gaaaaggctg gatatgagca cttcatgctg aaagagatct
    12601 atgagcagcc gaagaccatc aacgatacca tctctccgag aatcaaggat aatgatattg
    12661 tgatcgaaga gctgaacatg agtgatgaag agatcaaaga gatcacgaag atccatattg
    12721 tggcctgcgg ctccgcatat cataccggag ttaccgcaaa atatgtgatc gagggtctgg
    12781 caagaatccc ggtagaagtg gatgtggcca gtgaattccg ttatcgtgat ccgattcttc
    12841 agaagggtaa tatggtcatt gtcatcagcc agtccggtga gacggcagat actctggcag
    12901 cactgagaga gtccagggaa aggggcttca aggtactggg cattgtcaat gtggtgggaa
    12961 gctccattgc cagagaggca gacagtgtta tgtatacctg ggcaggtccc gagatcgcag
    13021 tggctaccac caaggcttac agtgcacagc taactgcact ttacatgctg gctatgaaat
    13081 tcgccagggt acgtggtact attgatcagg agaagttcgc cggtatgctg acagatttaa
    13141 gatgcctgcc ggatcagatt gaactgcttc tgggacagaa ggagaagatt cagcattttg
    13201 ccaatcgtta cgtgggagca agagatattt tcttcatcgg acgcggtatt gactacgcaa
    13261 tctcgctgga aggatccctg aaactgaaag agatctccta tattcattct gaggcttatg
    13321 cagccggaga actgaagcat ggtacgattt ctctgatcga agatgggacg ctggtagtgg
    13381 ctttggcaac ccagccggat ctgttccaga agaccatcag taatatggta gaagtaaaag
    13441 caagaggagc ctttgttatg gcagtcacca ttgaaggcaa caaggccatt gagaaagcgg
    13501 cggattatgt aatatacatc cccgagacca atccgtactt taccaattct ctggcaatca
    13561 ttcctttgca gttgttctcc tactatgtgg cagtgggacg cggctgtgac gtggataaac
    13621 ccagaaacct ggcaaaatcc gttactgttg agtagagtgt aataacttac ccatcgagag
    13681 actgcttgca ggcagaacag actctctgtt ttttcaggat tcataattgc ttcagaaagt
    13741 tatcacaatt cggacacaat tgacggatat actgtgttca taaggtgaga ggaagaaatg
    13801 aaacaggtaa tggtaaaaag agtttctcac ttgcaacagg ataggaggaa gcaatatgta
    13861 cgagaatgat agtttttccg gttacggaac cggcaatgaa aatacaaccg gtacattcag
    13921 tggagcagat ctgaatgaga caagaacaga aaacagttat ggctatgaaa atcatagtac
    13981 tacccagagt gcagcaggaa gcaccaatgg ttacaccact tatcagatgg gcggaaccac
    14041 aggcagcaac aatacggagc ctaagaaacc ccaaaaacat ggcggttact tccggaaggc
    14101 aatggtaagt gtcagcctgg gattgttctt tggactgttc gcaggaatcg gtttctatgc
    14161 agtacagcag ggaaccggga tgtttacgac cagcacagat accgcagtgg tagatgagat
    14221 cgcttctgag gcaacgacag aaagtaccca gagtgcacag ctggctacca atgttactta
    14281 tgtagagtcc gatgtatcca atgtggtaga aaaagtaatg cctgccatgg tatccatcgt
    14341 aaataacttt accgagacag ccaatgtctt cggacagcag tatacacagg aagaagcagc
    14401 cagcggttcc ggtatcattg taggtaagac cgatgatgaa ctgctgatcg tatccaataa
    14461 tcatgtagta gagagtgcag atactctgac cgttactttt atcgatggca gtgaagcaca
    14521 ggcacaggtc aaaggactgg attccgacat ggatctggca gtgatcgcag tatctttaaa
    14581 tgatctgagt gatgatacca agaatgctat tacagtagca acacttggca gcagtgatga
    14641 tctgaagctt ggtgagccgg tcatcgctat cggtaatgcc cttggctatg gtcagtctgt
    14701 taccaacggt attgtaagtg ccctgaatcg tgagatcact ctggagaacg gctctaccgg
    14761 tctggagaac ggctctaccg gtactttcat ccagaccaac gcagccatca accccggtaa
    14821 ctccggcggt gcactgctga acatgaatgg tgaggttatc ggtatcaact ccaacaagat
    14881 tggtggtact gccgtagaag gtatgggata tgctatcccg attacttcgg caagtcctat
    14941 cattgcagat ctgatggagc gtcagaccag aaccaaggta gctgaggatg aagtcggtta
    15001 catcggcatc tccctgcagg aagtaacttc ccagatctcc cagatgtaca acatgccgga
    15061 aggtatctat gtagtatctg tagaggaagg cagcgcagca gccaatgccg gtatcatgaa
    15121 aggtgatatc attaccaaat ttgacggcag cagtatctct tcctactctg atctgcagaa
    15181 aacgttacag tattatgcag tcggtgattc cgtaaccgta acggtacaga gacctcagaa
    15241 cggtgaatat gtatccatcg agctgaatct gacactgggc agcagacctg caagccagaa
    15301 ataaagaaat aacaggtaaa taaaaggaaa tggaaagaga cccgggcgag acatatggtt
    15361 ttgcccgggt cttgtaatat ttaccaaaat atgaatagtt ataaatggtt tagaaaaact
    15421 taacttgtgc cccgctactt cattgagtat aataagcgta ggcaatgaga agtgtcagac
    15481 acgggtatta tgcaatatga ctgcttgcag gcattattgc gtaatggccg gggatgatag
    15541 ggcgaagccc ggatatcgag ggaacacgaa gtgttgccga gatacactgc gaagatgctt
    15601 tggagccggg gatgataggg cgaagcccgg atatcgaggg aacacgaagt gttaccgaga
    15661 tacactgcgg aatagtggta ggaaacaaag aggagaataa catgacagat aattataatg
    15721 atgattatag agaagtgatc cctgaggagg aaaaaacaga gaccgatgtg agtgaaactc
    15781 aggataacga aaaaaataat ggaaacacac aggtggcgga tactgcaaaa gaaaattcca
    15841 ccgaaaaaaa gtcagaggac aaaaaggaca gcagcgaata cgaagatgtc tgctttatct
    15901 gccgcagacc ggagagcaaa gccggcagaa tgttcaagct gcccaaccat atctgcatct
    15961 gcgatgattg catgcacaag accatggaga ctgtgagcca gttcgattat cagggaatgt
    16021 tgaacaatcc caatgatttc aaccagttta acggacagaa cggattcccc aacatcagtt
    16081 ttgtgaatct ggcggatctt cagggagacg gcggtattcc caacaagcag aaactcaaga
    16141 agaaaaagaa ggcggaaggt cccaaaccgg tattcaatat caaggacatt ccggcacctc
    16201 ataagatcaa ggccagtctg gatgaatacg tggtaggaca ggaaaaagcc aagaaggtca
    16261 tctctgtagc agtatacaac cattacaaga gagtggccac tgacactatg gatgagattg
    16321 agatcgaaaa gtccaacatc ctcatgatcg gccccaccgg ttccggtaag acctatctgg
    16381 taaagacact ggcaaaactt ctggatgtgc ctctggctat tgctgatgcc acctctctga
    16441 ctgaggccgg ttatatcggt gatgatatcg agagcgtggt atccaaattg ctggcagcgg
    16501 cgggcaatga cgtagaaaag gcggaaaaag gtatcatctt catcgatgag atcgacaaga
    16561 tcgccaagaa gaaaaacacc aactcccggg atgtcagcgg agagagtgta cagcagggta
    16621 tgttgaagct tctggaaggt gcggaagtgg aggtgccggt aggtgccaac agcaaaaatg
    16681 ccatggtacc tctggcaacg gtgaacaccc gtaacattct gtttatctgc ggtggtgcgt
    16741 tccctgatct ggacgagatc atcaaggagc gcctgaccaa gaccgcatcc atcggtttca
    16801 actcggaact gaaggataaa tacgataaag ataccgatat tttatccaag gttactgtgg
    16861 aagatatccg caagtttggt atgattccgg aatttatcgg tcgtctgccg atcatttgta
    16921 ctttacaggg attgtcaaag gatatgttgg tgaagatcct gaaagagccg aagaatgcga
    16981 ttcttaagca gtaccagaag ctgttggagc tggatgaggt aaaactggag ttcacagacg
    17041 atgccctgga tgccattgcc gagaaggcaa tgaagcgtga taccggtgcc agagccctgc
    17101 gctccatcat cgaggacttc atgctggata tcatgtatga gatcccgaag gatgataaca
    17161 tcggaagggt taccattacc agagagtaca ttgaaggaac cggcggcccc atcatcgaca
    17221 tccgcagtaa tgagatcctg gaacccgtgg agaatctgaa gcagattccg gtggaagaga
    17281 aataaattga gagttagtga aatacattgg caaaatccac gaactcccgg agcat
//
LOCUS       DAWDHD010000039        17192 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_223_length_17192_cov_11.585984, whole genome shotgun sequence.
ACCESSION   DAWDHD010000039 DAWDHD010000000
VERSION     DAWDHD010000039.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 17192)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 17192)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..17192
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_223_length_17192_cov_11.585984"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    11579..11678
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4443 a         3591 c         4565 g         4493 t
ORIGIN      
        1 tagaacacca gccttccaag ctggatacgt gggttcgatt cccatcaccc gcttttaaaa
       61 acccttgatt tttcaagggt ttttaatttt catgttgcat ccattccaaa aaggtacgtc
      121 gattctcttt agtatccttt ttatcaaaca aaagtctcca ggcattatat ttttcataat
      181 taaaaacttg ttcaaggtga tttatcggtt tgcccggtaa tcagcgctgg tgttggtgca
      241 ctgggctgat tttttatttc aattttttct tttgctctct ctctaattgc tgaatacttt
      301 tttccggtgt aggcaaatct tccggcattg tgccaccaag atctttaatg gtgtcacgca
      361 cctttttacc aacctcaaag tgggttttat tcgcttcctg tttgccttga atattttcac
      421 ggcggagttt tgcttccgtc tgagtagcac ggaagaggtt ggcagctaat tcttcatatc
      481 ccatgtaatc gagtatgtct tgtgatttct ttaagccttt acgctccttt atatcgctag
      541 cctttaaacc accatatagt cccatatatc cgtaattttg aaatatagca taatccaggg
      601 tagtttcaac accagcatcc ttggcagcag ctactaacat tttattatgc tctttcattt
      661 cctgccggat ggcgatacgt ttttgatctt cggaaagatt ttcataattt tcaattagtt
      721 cttgctgtct ggtttttact gcaaagtagg tttgtccaag tgcaataacc ttttttcgag
      781 gatcaccatt ttgcacaata agataacaag catatcgtga aagttgaata tctccgatat
      841 ccttactggt ggcaccagca tttacgattt tgttgacgtc aacaaaatgg tcagataccg
      901 cgttattact acctcggcag gcttcttttg ccttttcaat aaccttgcaa aagtttctcc
      961 actcagtata ttccagagcg atttgtaaat ctctggcata ccaaaattct tgcccgtatt
     1021 tatttacatg acgaatagat tcaaatagtg actcggtata attatttttc tcttcgtcag
     1081 aaagagcttt cgagagcatg cggtcgtgca aagcatttaa ctgtttttca aattcttgtt
     1141 cgttcattac aattctcctt tgtatgtaaa aagagccctg gaatacaatc caagactctt
     1201 tcgcgaccgg acaccagtca ttcgctaatt agcggaataa taccattatg tgcaagcctt
     1261 gtcaagttat tcctaaaatt tatatttttg aaaaattgca ccggtgcaac aacatcagag
     1321 tttctcgaat accatagtag cctggatccg gtctccgccg ccgaagccgg tactattggc
     1381 agaagcggtg gtaatagtgt ggtgcaggcg gtatctcttt gcagcctggg cgttgatcac
     1441 atcctcgagc tccttcagat tcttggatgc agttccgata agtttctcct taagtgtcac
     1501 ttgtaatact acatactgat tcatagtgat tcccatttct tttgtgcgcc ccgatctcga
     1561 tcatctcttt tacaaactga atgtcctccg gcttcacttt ccgtgaggca tcgaagagga
     1621 ccttgtattc cggattctcg aagaggaact gagccatgtc acgggcttct ggattgagat
     1681 aatagctatc cggaataatt tcagtagatt cttttttcca ataaggtaat tcatgtcgac
     1741 attgagtaaa tcacccatga ggtgtttcct tccaattaaa ttagtagagc tgcactacaa
     1801 taattgtaga aaacatatgt tcccgtgtca agaaaaaaag cagcattttt tgacctggaa
     1861 caattacata aaacccaaaa ttaggtttga ctttcaagag atcttgtcta agcagaatga
     1921 tgctttgttg ataagtacgt gaaagagctg tgtgcggatg accaattata acaaggtgcc
     1981 ccgggagact gaataaaata gtgaatacct ccgatataca tgatagaaca ggtagacccg
     2041 gaaaacagga acggaggcgc ttatgcaggt cggtggagtc ggaagcggac atgacacata
     2101 ttctcatcat gtaacatcat gtcttcatga acatggggca aatacggata cgggcagtat
     2161 gggggctaag tccggagccg cgcagacggc gcagatggct acagaagcct tgcaggagaa
     2221 ttctaaggga aacctgttgt cctgggtaca gaatattggg aatagtggaa aacagctctt
     2281 acagaagatc tggggagaca gcaaagaatc caatgacgcg ataagttcac agaaggatgg
     2341 cagcggtaat catattgcgg gtcaggagac gcttgttacg ggaaattctg tgggcgtgaa
     2401 gactacgttg aacagggcgg cggtggagac ggcggcaacg catacctatt tccagccgat
     2461 agagacacga gaggctgtgc cgacaacacc gttgcagaaa gtgcgttatc aggtacagaa
     2521 ggtagccaga aaactgatgg accgactgcc gggacgattc ctgggatccc atagcggaga
     2581 tttcctgcag acaaagcaac agcatactaa agaggatctg cggaagagaa gccagtacaa
     2641 agaggacgat caggagattg aatgcattct tacggatgaa agctacctga cagattccta
     2701 taatcgaaag ggagaataca gccatcttac tacggataaa taggatgtgg tggaattttt
     2761 cgaaaaattt caaaaatttt cttgacagtt tggcattcat catgtaatat agtttttgct
     2821 gtgagacaca cagcagacat tcatcagatt gcggatgaat gtcaacacaa ttaatttcat
     2881 accttcaaga tacaggtttt gacctgtaca ttatgcgcga gtggctcagc ggtggagcac
     2941 ctccttgcca aggagggggt cgcgggttcg atccccgtct cgcgctctca tgaaaaaaag
     3001 aacgatacca aatcggtatc gttctttttt tcgagccctg ccagggctcg aaagttcgat
     3061 gtctacgctc cgctccggtc agcacttctt tctaataaat tctgtgtttc acaatacctg
     3121 tgtctgatcg tacagtgggt gacataatat atcggggttt gtatttttct aaaatgatga
     3181 atgggaaagc cactggtgac tttcccattc tattttctat tctactatga tatgtaacat
     3241 gccattttca tcttctgtta ctattgttcc acccaattca ccattttcat acattcttaa
     3301 taatatcctg ttttcagcca tacgatattc ctgatggttg gctcctgtga atagggaacc
     3361 atcaggtaag taatctccta cgtggaagac ttctccatat cgtggggagc cttctttcat
     3421 gtaaacggca tagctttctt cggaacctgc tcctttgcca gtgtcgtaaa cccagcacca
     3481 acctatagtt ccatctgctc cttgagattg tgcatatccc gtccacggtt cataaccacc
     3541 atattcttct gtcacttcat taaattcttg ttttccaagg gcaaaggcga tgtctccatc
     3601 tatgttggtt gccttgggat ctacatcctc cggatttaca tcaggataaa tgatgttctc
     3661 ggcaatttca tccattaaac taccatcacc tgctacatga taatacatga atgacattgt
     3721 cgtaaataat ctgaccgctt caccttttgt aattgctgca tcccaatctt ctaagtctga
     3781 ttctgaaatt aactccatat tagtagctat ttcaaatgct ttatacatat cagatggaac
     3841 tcccttacca ggatcagaga ttgcatctga aaaactgatg tctcctgcat cggatacggt
     3901 ggatattact acatcttcaa gatagatgtt gtttttaaaa ccatgtttat ccatatatgt
     3961 ctggtagtct tggctataat agtttgctaa cagataaatg tactctcctc ttgacattgc
     4021 tgacgtaaaa gtctcttcat ctaaaccagt cgaagtattc acataggcat attcatccat
     4081 tggtgcggca aaattggtat attgtgtttc tcctactttt gctgtgaagt cactgtctgt
     4141 ttctggtgcc tgtgcttcat ttacctgagt cattgctctc attgttaacg tcattgcctg
     4201 tgctcttgaa agggggacat ctccatcaaa ctgtctttct tcttggtctg gaagcaattc
     4261 aaagtaggca ttgatagttg catagggagc attcgcataa tctgcatcta tatctgcata
     4321 atattctatt gccatttcac caagctctaa aacagacgac actaccatca tatagcctcg
     4381 aaaatattga ttttttattg ctaaaagtaa cgtgatattc tggtctggcc gtgttgttaa
     4441 tggattcgtg tataagttac cctctttgtt gcctgtggtt cctgttactt ctacatattc
     4501 ctcaaaggct gctcttaact ccggatgtgt ttctaaactt gagagtggaa tccaggggag
     4561 tgacaccgtt tttgattctt ctgtttctgt ttcctccggc actgtggttt ctgcctgtgt
     4621 ggattcaaca gtagcagtga tggattcatt gccggatgtg gtttcctgct tgccacagga
     4681 agcaaaagat aaaatacata cggttatcaa ggttaaaata gttgtttttt tcatgagtta
     4741 atgttattgt accacagaag ggggaataac gctataacgt ttattatgtg tgatgcacat
     4801 gaccgcggga ccagacttta ctgggctggg ctttgtaggt ggggcggcgg aacaggaggc
     4861 ttcggagagc cgggccgtta aaaggaaaga ggggccagaa gtagctgaag cccgcgaagg
     4921 tcggggtagt caaaatagag actgtcacca gtgtcagacc gataaggaat cccggcaatc
     4981 cccagagccc tgttgtgacg accaaaagaa tgcggtagat ccgcagagca tcagcaaatt
     5041 cgatactgga gagggacaga ttcgccagca tggtgacggc agcatagaag aggacttccg
     5101 tggaggccca gttaagactg acagcaatgt caccgattaa tagtccgccg acaatggaca
     5161 gagcaccgga gacgcggctg gagctgagtg ccgctgaata cttgaaaaga tccagcagga
     5221 actccaccgc tagcacatag aaaaaaaggc ggaagggtga cagatcgctg acgggtgtga
     5281 gctgcagcga ggcagtaatc tcgggatagt aggcggagag aagtaaaaat accggaagga
     5341 gtaggagact gaccggaatg cacaggaagc gtatcatgcg gaagtaggtt ccggtcagag
     5401 gattgttgta atagtcctcc ggactctggg taaactggaa gatcgtaccg ggaagcagca
     5461 gaacggcagg ggagttgtcc acgatgagca gaatatgccc ttcgcacaga tagctgcagg
     5521 ccacatcggg ccgttccgtc agctggatac tgggcagagg attccaccag cgttttttga
     5581 tcaggagttc ttccagactt ttgctgccca tggtcaggga agtgatctgt aattgggaaa
     5641 gctttttctt taaggtatct aataattcct cattgacctg atccgccaga taggcgatag
     5701 ctacatccgt gcggctttct gcacccagag aacagataga gaatgtcagt ttcggagaac
     5761 gcaccctccg gcggatcaga ttggtattga acaacaacgt ctccacaaaa ccgtcccggg
     5821 caccacgggt gatccgttcg gtgtcaggtt cggaaatgct gcggacagga taggtacgga
     5881 catcgatgat cacggcctgt gcaaaacctt ctaccagaag gacagagggg ccactgagaa
     5941 tattttgcag aatcgtatct atagaggaag tcagtgacac ctgggcatac cccagtttgc
     6001 tctgcacata gcgagggaga tcccggattt cggaatccag tgtatagagg gggtcctgca
     6061 agtcggaaaa aatttgttga aggatctccg tcttacaaaa accattgaca ccgatccagt
     6121 aagccggcgt atttcccagc ttcaggtcac gggtgatcag gtcaaaactg tcatccagtg
     6181 gcagcagagc gtggaaaagt gctgtatttt tggatagatt tgtggataag gagcgttgta
     6241 ccatagtggg gaatcctttc tgattacttg catagactgc caaacaggta tatggtatta
     6301 ttattcagat agattcccca ggtttttcgg aactatacgg atcagtggta taattgacac
     6361 aaccccagca ggatcaacaa taaacctgag acgggatccg tatattcccc gatctgctgc
     6421 aggaaagcag acctgcccag acggcttccg aaagccagaa atccgaagga gcagaaaaag
     6481 gtggacaccg atgccggaag gaaaggaagt cccgtaatac tggcggagaa accgatgccg
     6541 atattgttca tggaaagggc acagcccagc gccagtatct gcgacaggga taattcccgg
     6601 tgcccggcaa aatttttctt cttctggcac agattattat ccggggaaaa attgcagtgc
     6661 catctggaaa gcagccattt tcccacgtag taaagaccga aaaggatcag tacactgctt
     6721 ccgatccggg taccgatgcc gatgggaaac aagggtaaca acaggttgcc aagagctata
     6781 gagatcacag tcccgatcag ggtgactgtg ctgatcagca gattatgtct ggcaggcaga
     6841 gggactttct ggaggcccag agccatacct accagaaggg catccagact ggctgagatg
     6901 ccgaagagca gagggggcag aatagacata acatgctccg tagaaaatat tcttaatttt
     6961 actatattcc cctgaaatta ttttgtgagg ttgtggggca aaggaacaaa tgagtatgga
     7021 agtaaagaga aaactgagtg aattcctaaa aagagagacg gttctgaccg tagcgatcct
     7081 gctggcaata gtgtccgcgg tgatgattcc gccggacaga gaatatgcag gatatattga
     7141 tttccgcaca ctttcgatcc ttttctgcct gatgacggtg atggcaggct tgcagaaact
     7201 gggtgttttc cgtaatattg caaaaacgct gttgcggcat acacagaata ccatacagtt
     7261 ggcagaggta ttggtactat tgtgtttcat tttcagtatg atcatcacca atgatgtgtc
     7321 attgatcacg tttgtaccgt ttaccttcat tgtgctgcag ctgacaggcg aagaagctgt
     7381 gaaaaaactg gcagtacctg tgatcgttct gcagacgata gctgccaatc tgggaagtat
     7441 gctgacgccg atcggaaatc cgcagaatct gtatttatat gggaaaacac agatgtccgc
     7501 tggaacattt atacttttga tgctgcctta ttctctggtg tcactgcttt tactgatgat
     7561 ctgtgtggta attgtggcaa aacgaagcgg aatcgaagtc cggggagcag aagttttgct
     7621 gacagaagat gagaagctgg aacaaaagaa atatttactt ccggcatatt tgctgttatt
     7681 tgtgttgtgc ctgctgactg tggctcatat gattccttat ccggtgacat tggggactgt
     7741 ggcattggcg gttctccttt tagatcgtgg tactcttata aaagtggatt attcgctttt
     7801 gctgacgttt gtgggcttct ttatttttat aggaaatatg ggaagaatgc ctgctttctg
     7861 tgatttcctg cagaagatca tcggaggtag agaagtgatg acagccgtga ttgccagtca
     7921 ggtgatcagt aatgttccgg cggccctgct gttatccgga tttacggaaa atataacggc
     7981 attgatcatc ggtacgaatc tgggagggtt ggggacgctg atcgcatcca tggccagtct
     8041 gatctcttat aaacaggtgg ccagacagat tccgggagag aaaaagaagt attttggatg
     8101 gtttaccata gcaaatatcg tttttttaat gatattggta ttggaaaact gtctgctgta
     8161 actatagaaa gcagtctgtc tgccgggaaa ggcagaaaag atggcaataa ctaaaaaata
     8221 atctgggagg tattcgtcat gacagaagat atgacaatca ttgcagaaga ggcttatctg
     8281 gcggccaagg agatcttgga aaaggcgagg ctggagcagg gagagctgtt tgtggtaggc
     8341 tgttctacca gtgaagtggg aggtgctact atcggtacct attccagccc ggagctggcg
     8401 gagatggtct tcggcggtat ttaccaggcc acgcaggaag ccggggtata tctggctgcg
     8461 cagtgttgtg agcatctgaa ccgcgcactg attctggaaa aagaggctgc ggtaaaatat
     8521 ggttatccca tggtaaatgt ggtacctcag cctaaggcag gcggatcttt tgcgacggcg
     8581 gcgtataaag ccttcgagca tccggtggct gtggaacata ttcaggcggc agcaggaatg
     8641 gatatcgggg atacgctgat cggaatgcat ctgagagatg tggcagtacc ggtgcgcctc
     8701 cggtccggac agatcggaga cgcccatgtg gtatgcgccc gcaccagacc gaaattcatt
     8761 ggtggcggca gagcggttta tgacgaagaa ttattgtaaa gaaatgatat gtggctattt
     8821 ctgtggcatt ccgcggaagt agtcacggat ccagcgtccg gattcattga aatcatcgtc
     8881 ggtccagtcg gaaagtgccg tgctggaagc cttgaagacg ctgctgcttt cgtctttgtt
     8941 ggcgagattc cagcagcaga agctgatctg atatttgttc agcagatcca gccacttggt
     9001 cgtggaatca aaatcgttgg caccattgcc ggaggcatcg cacatgccaa attcgctgac
     9061 aaataccgga agcccgttct gaacacaggt ctctaagcgg ttccgcaggt cgtctttatg
     9121 ggtgcctgcg tagaaatgca gcgtatacat tacattgctg tcatccagcg gagaagcggc
     9181 agccttgtcg atctcctgac tccaggttgg tgttcccacg agaattacgg catccggctt
     9241 ctgcgcccgg atcaccggga tgacttcttc tgcgtaagac ttgatgctgt cccaggaagt
     9301 accgccgttg ggttcattgc agatctcata gagcacattg tcgttgtcgg caaaaacttc
     9361 cgccatttcc cggaaaaagg cgatcgcctc atctttgttc tgattcgggt cgcaatcact
     9421 taaaatatgc cagtctacga tcacgtacat tccaagctct gtggcgtagg atacaccgtc
     9481 tcttaccagc tgcttcagct gttccttatc accgccggca caataaccgc cgtattccgc
     9541 tgtatacatg gcgaggcgga tacagttagt attccagtcg tcccgcaggg tacggaagga
     9601 atcataattg atatactggg ggaaccaggc aataccgtgg gtggacatac cgtagagctg
     9661 gacagtgttc ccgtctgcat cggtcagctt cccgttctcc acatggaggg caccgtgctg
     9721 tccgaagggg gtggatgcct tatctttgtc agcctcggaa gttacatccg caggccgtgt
     9781 ggattgcgtt ccgggagcat cgggggccgg tgtctgttcg gatgcagcgg tgctttcgtt
     9841 aacggtttct gtgatggcag cagacgcttc gggagaagca gatgttgcgg attcatttga
     9901 cgtatcctgc ctgccacagc cggcgagagc acaggtgcct aaggctgcca gtacggcaca
     9961 gctgagtgtt tttctgaaaa tagacataat aacctctttt cctatttgtt tgagaataaa
    10021 cgaatgaaaa tacaaacaat gggttacctt ttaaaactaa aaacaagcac ttacaacaaa
    10081 tattataatt gtgtctgtgt agcttggcaa gaaatgattg cccccgaata ggagctgtga
    10141 tacagttaat gggaacagag ttcggaatag aggacagaga tggaggaaac ttatgcttcc
    10201 gcagtcattt acagagagaa tgcagggact tttggaatcg gaatatgaag agttcctggc
    10261 aagctttgag catgaaaaat atcaggcatt gcgtctgaat ccgttaaaaa aggatgacat
    10321 gtctgtcata acagaaaaag ttaagcagac ttttcagctg cagccggttc cctgggcgga
    10381 gaacggatat tactatacca aagaggacca gccgggaaaa catccctggc atgaagcggg
    10441 actttattat attcaggaac ccagtgccat ggcacctgtg acactactgt caccacagcc
    10501 cggagagcgg atcctcgatc tatgtgctgc tcccggaggg aaaagtaccc agatcgcatc
    10561 agcgatggag ggagaaggac tgcttgtcac gaatgagatc cacccggcga gggcgaaaat
    10621 actctcggag aacgtggagc gcatgggcat ccgcaacgcc tgtgtattga atgagacgcc
    10681 ggagcacctg gcggatattt tcgaggaata ttttgaccgg atcctggtgg atgcaccctg
    10741 ctccggagag ggaatgttcc ggaagaatga agtggcctgt gaggaatgga gcccggaaaa
    10801 tgtacagctc tgtgcggaca gacaggacgg cattctggaa tgtgccgccc ggatgctggt
    10861 acccggcggc agactggtgt attctacctg cacctttgca cctgcggaaa atgagggaag
    10921 tatcagccgt ttcttggcaa agcatgagga attcgagatt gtccccatag ataaaaaaac
    10981 actggggatc ccggaggggt gtgacggtat tccgggctgt gtggagaacc cggcgccggg
    11041 tatcgaaggc accttgcgcc tgtggcccca taagttacat ggcgaggggc attatgccgc
    11101 agtattacag aaaaagggag agctcccgga aggctgtcag ccggtgagtg ccaccggacc
    11161 agagaaggga attccggcga agaatctgac aaaggactgg gcagagtatt ttaacttcgc
    11221 aaaagagact ttttcggagg agcagatgat caaagcagga ctatgcactg ccggagaagg
    11281 ctttctggca tttggtgata acctgtaccg catgccggag agaatgcccg ggttgaaagg
    11341 actgaaggta ttgcgtcccg gactgcatct gggcaccttg aaaaagaacc gttttgaacc
    11401 ggcgcacgcg ttagcactgg cactgcgccc ggaggatgtg aagcatgtgt ggaagctgtc
    11461 tgaggaggaa gccgctgctt atctgaaggg acagactttt tccgcggaag gcgagaaggg
    11521 ctggtatctg atctgcgtgg acggctgtag ccttggctgg gggaagctgg ccggaggcnn
    11581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngt atctgatctg cgtggacggc
    11701 tgtagccttg gctgggggaa gctggccgga ggcatcatga agaatcatta tcctaaggga
    11761 ctgagaaagt gattaggcga tagagatgca acggtatagc ttaacatctg gaacaacggt
    11821 tagggggatg acagaggaat aggttatgaa aacggaaata ttatatgaag ataacagcat
    11881 actggtcatc cgtaaaccgg cgggactggc ggtgcagtcc gccggaatcg gacaaccgga
    11941 tgtggtcagc gagttgaaat cccatcttgc aaagcttccc ggcgcgggga ggccgggaaa
    12001 aggagagccg tatctgggca tagtccacag actggatcag ccggtggagg gcgtgctggt
    12061 atttgccaaa gagaaaaagt cggcagcagt tctgacgaaa caattgtcgg cgggcacatt
    12121 gaataaacag tattatgcag tactctgtgg atatcctgat gcttcggaag gggagcttgt
    12181 ggattatcta cggaaagagg gcagcgtggc agtggcagtc accggcagag agaagaattt
    12241 tccggacgca aagatcgcaa aactgcatta tagcattttg gagaaaatta atcagcctat
    12301 gccgctggca ctggcagatg tctgcattga gaccggacgg ttccaccaga tccgcgtgca
    12361 gtttgcccat gcgggatggc cgttgttggg agataccaaa tacgggaaca ctacagtcgc
    12421 cggaacggcg gggagtccgg catatcgcgg cgtggcactt tgcgcatatt ctctggattt
    12481 tacccatccc ggcaacggca aaaagatgtc attccgggtg aaaccgcaga atcctgcatt
    12541 tgaaaaattt acaaaaattc cttcggagga tattttcgaa ggataaaaaa gcctgtccgg
    12601 cacacactaa tcccaaaaag gaagtgtgcg gcatggattg gggagaattt ggcaaaaaca
    12661 taataggaaa aaagatgata gtactgcttc tggtaacggg ggcagtctat ttttttctgc
    12721 aatatctgac acctctgtcg gcaccggtac tgttggccat gctgtttgtc acaatttttg
    12781 ggccaatgat gaagcggatg aatcagcgtc tgcattttcc cagacccctc agtgccatag
    12841 tgattctcat cgtggcaaca gtgttgctgg tggggttact ctggattctg gtgtcctgga
    12901 tgctaggcag cctgccggta tggatcggca atctggaact gcgggagcag gagtggctgg
    12961 aagccgtccg ggggctgtgt cgcagcgtgg gagagattat tggtgtggac actgcgtatc
    13021 tggaaaatac ggtcagtggc aggctacagg aggggctgga ttcactggaa gaaaatattc
    13081 tgccgggaat gctttcccag tctatgcagt atgtgcagtg gttcgcggag ctgggaggat
    13141 ttctggtgac gtttttgatc gcttcggtgc tgctggcgaa agactatgac ggtattatga
    13201 accgtctgct ggatcaggag gcctgtcatg tacttctgga ggtgatctgc gggatcgtcc
    13261 gttatattgc cacttttgta aaggcccagg ggatcattat gaccacgatt gccatgacag
    13321 ccgcggcaac attgggaatc gccggaataa aacatggtat tttgtggggg atcctggcgg
    13381 ggattttaga tgcgctgcct tttatcggaa cgggagtggt actggttccg ctgggcatgt
    13441 ttcagatcct ggatggcaac tggggccggg cactggtctg tggtgtattg tatgtggtct
    13501 gcatttttct gcgggaggtc atggagcctc gtctcatcgg caggaaaata ggggtatccc
    13561 ccatagcagt gctggtgtca ctgtatgcgg gaatccggct gttcggtgtc tgggggatca
    13621 ttaagggacc cctgggattt gtgctcattt atgagactta tcacagtctc gcccggatgt
    13681 ttgaccgcca gagggagcag gaacaacagg aatgagaaaa gattgacgaa agaagtgtcg
    13741 aaagctatca taaaagtatt agacaaagga agcttaggag agacggatct atggcaaaaa
    13801 aaacaaaagg gaaaaattct gtggaatcgg tggcagaaac gctatgcaat ttcagccggt
    13861 tcctgtgtga tatcgtgatc agcgtgtatc tgtttgtgat attggctgta ctgccgttgt
    13921 acaacagggg ctatgcccag atcggaacag acaaggagaa tttttttatt aagactatga
    13981 cttatgcggg gaaatgtctg cttccggtat ttgtgttgtg gctgatcctt cgcctcattg
    14041 tggcaaagca gaaacaggag cttccggaat ttcggactat tccgaaaaga atctggcagg
    14101 ctttgaacac cacggataaa ttcgctgctc tatacgggat tgccgtggta atgtcctatt
    14161 tgtttacccg gtatcgggag gaggcactgt gggggacggc atcctggcgg atggggatgt
    14221 ggacccagct gggagcggtc attgtatatt ttatgatctc acggatgtgg cagagaaacg
    14281 actggatcgt gatcctgaca ttgccggtgt cgaccattgt ttttttcctc ggatatatga
    14341 ataaattcgg gctgtgcctt gtagataagg agatcatcaa ttcctccttt atttctaccg
    14401 tgggaaatat taactggtat tgcggttatc tggtgacggt actgttcggt ggagtttatc
    14461 tgttatggtg gatgggatcg gagatcactt ggaagagagc gctgctgatg gggtatgtga
    14521 cgatcggttt tgcttctctg gtaacccagg gcagcagcag tggtgttgta actttagccg
    14581 taatgctgtt tgtattgttt ggcatgtctg tgaaagatgg cggaaaaatg gaatgcctct
    14641 ggcaggaaat gacattgtta tcggtgtcat gtctgatcac atatattctg cggagctgca
    14701 atgtgctttc gcaggaactg ccgatggaaa aagtgacgga tatcctgacg ttttccgcaa
    14761 tgagtgtgat tatgacgatc gtgtcagtag tggtgctgtg gctggtacat accagcaata
    14821 acagaaatca ttaccgtggg aaactttttt ggggcatcta tcggatattg tgcgtagcac
    14881 tgccggttgt gagcgtggca ttattagcgt ttattctggc gaatacgctg ttgggcggaa
    14941 aactgacgtc ctttctgacg gagacggcag gtgacggggg cagcaactgg ctgatgttca
    15001 gtgcggcctg gggcagcaac aggggagcca cctggtcggc gggagccagc tgcttctggg
    15061 aagcagatct gttacataag attttcggcg tagggccgga ttgcatgtat gcgttcctgt
    15121 cgaatgaggc cagcccggag ctgcaaaata tggtgtatga tttcttctat ggaaaccgcc
    15181 tcactaatgc acataatgaa tggctgacga tcctggtgga tataggagtg ctggggctgg
    15241 tcagttatgc gggaatgatg atctcagtaa tccgtaattt cctacatgcc gggaaaaagt
    15301 cgatgctggt gggagcctgc gggttctgtg tgctggccta tacggtgaac aatatgttca
    15361 gcttccagca ggtgatgaat atttcgacgg tgtttgtgat tatggggatt ggagagaatt
    15421 tgcggcgttc agcccgtgat tgaactagtg aaagttgaat ttgaggtctc cgctccgctt
    15481 cggtcggtcc aggccgaggt tccccggacc tcgtgaggtc tccgctccgc ttcggttggt
    15541 ccaggccgag gtcccccgga cctcgtggac cctctgcacc cgaacggtgt gtccgaacca
    15601 gttagaggtc gtaagtttga atagttgcaa gaacaaaaat actccctcac acatgtgtga
    15661 gggagtattt ttagtcgtcg cgacaagatt cgaacttgcg acctctgcgt cccgaacgca
    15721 gcgctctacc aaactgagcc acgcgacgat tttgttaaca cgaattgcgt caacattaaa
    15781 ataataaata aaaacccatt gattgtcaag caaaaaacga atatgataga agaaaaagtt
    15841 gcaaaaacga taaaaccacg aaattaagag aggaaattct atgtacaata caacggtaac
    15901 gctgaaaaaa ggagagggca gaacactgaa agccgggggg atgtgggtct ttgacaatga
    15961 gatcgcatcc attatgggag attttgagaa tggtgatatt gtaaacattc atgattttga
    16021 cggctatttc atgggatacg gttatatcaa taccaattcc aagatcacgc tccgtctgct
    16081 ggcgcgcagg aaggatacgg tgatcgatga ggcatttttt gagcagaggg tacgggatgc
    16141 ctggaattac cgcaaggaga ccattgatac ctccagctgt cgtctgatct tcggagaggc
    16201 ggatttcctg ccgggtattg tcattgataa gttctctgat gtactggtgg tggaatctct
    16261 ggcactggga atcgacaagt ggaagcttgt gatcattgat gctttgaaga aagtactggc
    16321 ggaggatggc attaccatcc gcggtgtcta cgagcggagc gatgccaagg tacgcttaca
    16381 ggagggtatg gagcgttaca agggcttcat cggcgaaccg tttgacacga aagtagagat
    16441 tgtggagaac ggtgtgcatt acatggtgga tgtagaggac ggacagaaga ccggattttt
    16501 cctggatcag aagaataacc gtgccgccat tcatcgtttc tgtaaggata aaaaggtgct
    16561 ggactgcttc acacacaccg gctcctttgc attgaacgcg ggaattgccg gagcgaaaag
    16621 tgttctgggt gtggatgctt cccagctggc tgtggatcag gcaatggaaa atgcgaagct
    16681 gaacggactg gaaaatacgg taagctttcg atgtgcggat gtcttcgagt tattgccgga
    16741 gctggaagcg cagggcgaga aatatgatgt ggtgatcctg gatccgcctg cttttaccaa
    16801 gtcccgcaat tccatcaaaa atgctgtaaa aggatatcgt gagatcaatc ttaggggctt
    16861 aaagctggtg aaggacggcg gatttctggt aacttgttcc tgctcccatt tcatggatcc
    16921 ggagcttttt gccaaaacaa tccgggaggc ttctcatggt gcgcataagc gtctccgcca
    16981 ggtagaattc aagacacagg gcccggatca tccgatcctg tgggcagcgg acgagagcta
    17041 ttacctcaag ttctacatct tccaggtggt ggatgaacgc taggcgctcg tcaggaaaag
    17101 taagcggctg aaagtggcgg aaatgcttga tttaaacaaa tcggaatttt tagaataaaa
    17161 tatttccata agattaagca gattttagta aa
//
LOCUS       DAWDHD010000040        17190 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_224_length_17190_cov_11.899621, whole genome shotgun sequence.
ACCESSION   DAWDHD010000040 DAWDHD010000000
VERSION     DAWDHD010000040.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 17190)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 17190)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..17190
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_224_length_17190_cov_11.899621"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         4132 a         4766 c         3053 g         5239 t
ORIGIN      
        1 ctgaaaatct tggaattttc agggttgcat tactgtttat ttgtcaaaga tcatagcctc
       61 ttgcttcgca agagctcgtt tgttcgttgc tgttgttctc agcggcaact ctgatatctt
      121 atcataaccg ctttcgtttg tcaacaactt ttttcgattt tttcaaatct ttttctcgac
      181 cggaaaactt tcattttcgc ggtggagttt catactacca cattgctccg tgctttgtca
      241 acaacttttt acatttattt ttcaaagcat ttacacaatg gtggaaatgg cgcaaaaaaa
      301 gaaatctcaa tctcttaaga ttcttctttt acttcctatt ggtagtaaac ggagaaagag
      361 ggatttgaac cctcgcgccg gttgcccgac ctacaccctt agcaggggcg cctcttcagc
      421 ctcttgagta tttctccatg aaccgaacat cgtctctacc aatatgttat tggcgtcatt
      481 tcgtgacgca aaagttatta tacataagct ctatgctttt gtcaattgct ttttttatta
      541 ttttcctaat tgtagatatt gtttatgaat attgccgaat agcttcttca atcttcgttt
      601 ttacaatgtc cgcaatctcc ggtgttttca actgtttata ctcttcataa cagattggcg
      661 gtaaatagat cagctttacc gtaaccggag ctatggactt ctcatcaaac ggtttgtaag
      721 aatcaatcag agcacatggt acaatggggc actttgctct gactgcgctt ttgaatgttc
      781 ctcccttaaa tggaagcagt tgatttccca tacggcttct ggttccttcc gggaagatca
      841 gaaagtttct gccctgcttt acttcttctg tcatctgatt gatcacctga agcgattgct
      901 taatgtcttc tctgtctatg gcaatcgcgt gcaatgcccg aaatacttgt tttaacagga
      961 ttacattgct tacttctttc ttataaacta cagagaaagg aaccgggcag gactccagaa
     1021 acacaagtgc atcaaacatt ccctgatgat ttgggaaaaa gataaacccg ttttctttcg
     1081 gaatattttc cacaccatag ctctctatgg ttactcttcc ggcacggttt gccgcttttg
     1141 ttaccttttt gataaatgca tagttctttt ccaggtcaaa attctttctg gttcccagcc
     1201 accatatccg aatcagataa tacggcacct tatagaacag tcttaccacc atcagtatga
     1261 ttctatacat agtccgcccc agtctactct tcgttatatt cttttaccgc cgtttcatac
     1321 aggttattac cttctgcgtc aattactaca atcgcaggaa agtcctttac ggtcagttta
     1381 cggatagctt ctgttccaag atcatcatat gcaatgacct cagaagaagt aattgcgcgg
     1441 gacaaaagtg ctcctgcacc acctacggct gcaaagtata ccgctccgtt tcttacaatg
     1501 gcatcccgga cttcctgtga cctttttcct tttccgatca tccccttcag tcccagatcc
     1561 aataatcccg gtgcatattt atccattctg ctggctgttg tcgggcctgc agaccctata
     1621 ggacggcctt ctctcgccgg agacggtccc atatagtaga ttacgttatt tttcattcct
     1681 ataggaagtg cttctcccct gtccagagct tcctgcattc tcttgtgcgc cgcatcccgg
     1741 gctgtataga tggtccctgt cagatataca taatctcctg atttcagaga gcgggcatct
     1801 tcatcgctga gaggtacttt tacatattta tccatgtcgc ttacactcca attattataa
     1861 tatccggaca tctttacttc agatctctct aactgcatgc ctgttgacat ggcaacagat
     1921 gtttacagct accggcaatc ccgctatatg tgtgggatat gtactaatat tgaccgccag
     1981 tgctgtggtt gatccgccca gtcctcccgg tccgatgcct gttttattaa tcttctccag
     2041 cagctcttct tccagttccc tcacataggg tatctccgaa tgttcatcca ccggacgtgt
     2101 caatgccttc ttcgccatca atgcacattt ttcaaaagta ccgccaatgc ccactccgac
     2161 taccatagga gggcacgcat tcggtcctgc atcctttacc gccgtcagga ttgcgttctt
     2221 cacgccttct attccgtctg cgggcttgag cataaatact ctgctcatat tctcactgcc
     2281 aaatcccttc ggtgccacgg ttatcctgac acgatccccc ggaacaatac tataatgaat
     2341 gatcgcgggt gtattatcct tcgtattctc ccggtaaatg ggatccttca ccacagattt
     2401 tcgcagataa ccgtccacat atccctgtct gacaccttcg ttgacggcat cttccacatt
     2461 gccccctgtc agatgcacat cctgacccac ttccagaaat acaaccgcca ttccggtatc
     2521 ctgacagatg ggaatcatat ccttgcctgc aatatccata ttctgctgca actgttgcag
     2581 gatctgcttc cccaaaggtg ctttttcctg ctgttccgct tttgtaaatg ccgtcttcat
     2641 gtcctcagat aagaaatgat tggcctctat gcacatttct ttgatatttt gcgtcaaagt
     2701 agctacatct actgttctca tggtttctcc ctgttattcg gtaatggttt tgcgaatctc
     2761 ttccagttct tccggtgtac tttccccggg attccataaa atatgctgtc catcattctc
     2821 ataacgggga atcaaatgca tatgaaaatg gcgaacggtc tgtccggcag cctctccatt
     2881 attctgtacg atattgagac catcgcaatg taatttctct gtcatctttg ttgccatctt
     2941 ttttgcaaga acaaatactt ttgccgctgt atcctccggc agttcataga gattatccgc
     3001 atgctccctg ggcaggatca gcgcatgtcc tctggtagcc ggtcccagat ccaagattac
     3061 tctgaaatcc ccgtcttcgt ataatgtctt ggaagggatc tctccgtttg caattttaca
     3121 gaaaatacaa tcttcctttt tcatcgtttt gctcctatct gttattttct tcaaagggaa
     3181 acatctgatc cagtgttctg agaattcgga aatctccctt cattcgcttc ataatgcctg
     3241 acataaacgc cctctggaag gtcattcgtc cgttcacaat atcttccatg gcagcacgat
     3301 tcatctgcat ctccacatcc ggcttctccg tctcaccgta tccgcattcc agtgcgccac
     3361 tgccaatagc gatccacaga ggattcttct tttcttctat attaataaca tatcttgttt
     3421 caaaaccagg ctgcggtaca aaaacttttc ggaactcttc cagatattcc tgttcattat
     3481 ccgttccgct gttatccaac atatcccgga acatggaagt caattcctga atatcttcct
     3541 tctgtcgctg tacaaacgta tcatccgata catattccga caactgttct gtctcctgcg
     3601 gtgtcaggtt gattgccttg gggatcgtta ctttctgacg catcacttta ttgctggccg
     3661 ggaaactggc aatcttctgg ttgatagtac ggtacatgtt ctcagccttt ttctcaatga
     3721 tctggccgta ctgtccgttc atctccaacg ttactgtatt ctctatatat ccgcatatgc
     3781 cgctgcaggg caatcctccc agaatctccc aggccgccgc aagactgagc ttgccttctc
     3841 tctctccgta ggtggtagac atcacgaccg gacacatata aattcccgcg atcgtctctt
     3901 tatccccata cagccagcag gcatccagaa actgctgcat atagccaccg ataccgtacc
     3961 actccactgt cgttgccaga ataattccgt ctgcctcttt cagcgtctgt ggcaatgtcg
     4021 taatattggt ctttccatca taaagattgt atctctccac atgcacccgc aactcttcca
     4081 gcacttcctg catcttattg atcacataga tcgtaggatc atcaatgatt cctcgtccgc
     4141 catagtaaat gttgatattc aagtccacac ctctttcgtt ttcttctgtc ttctgtgttt
     4201 cctgagcgca caaaacaaaa gtcccgccag gaatccggtc accagtccgc ccacatgtgc
     4261 cgcattgtca atattttgtg cagtaaaacc actgtacaga gataatgcta tcatcagaat
     4321 cacccgtttg ggtgtcacat ccggcataga ttgccgatcc agcaaaatcc aggcaagcag
     4381 tactccgtcc agtccaaaga tggctccgct ggcacctaca gatgccgaat aatccatctg
     4441 cagaagcttt accagcagtg agcataaatt ccctccgatt ccggacagaa aataaagcac
     4501 cagaaacgtg acatgacctg tcaccttctc aatcatggcc cccagaaaaa acaggaggac
     4561 catattatta aaaatatgct ctatgcccgc gtgcaaaaac attgcccaca aaagcctgcc
     4621 gtactgttta ccggccaata cactgactac atccagttcg cctttattat ataacacatt
     4681 tcccgaaaaa gtacacatca ggaacacaat tacattgatc accacaagca ttccgcttac
     4741 ccagggtaca ttacgaagcc ttttcacctt cattgctcct ctcaatgttc tctccatccg
     4801 gtattgtatt tcaaatgtac cactgtttat atcactccgt caacaaaaag ggggctgcac
     4861 actgcctacc ggtagattac cgtgatctta cccaatgtaa tctcatctcc ttcttccaac
     4921 tttcgcctct cataaggctg cagttgtaat ccgttcttaa atgtaccgtt tgtggaattc
     4981 atatcttcaa gataataccc atctgtttcc tttatgatcc ttgcatgcag acgactgatc
     5041 gactgatcat tcaataccag atccgtttct ccccgttttt ttccgatcgt gtagattggt
     5101 tgttctaatg ttgcaagcag cttcccttct tccgacaaca ggcggtgtgt actctctctg
     5161 ggggccggtc tttcctccat atacacagtc cttcccatat cttcttcctc ataagcggtc
     5221 tcctccgcca ccgcatatcc cgtcatggtc agttgcagac tccgggtata attatcccgt
     5281 tcctgcttct ctttcttttt ccggttttcc cacagattaa acaggccttt cttttccttt
     5341 attactgtaa cttctcccac cggcatttcc ttctcttctt cccttatcgc atccttctcc
     5401 tgatcttgtc ccacgacaac agttgtttcc tcctctacgg catccatttt ttcagggatt
     5461 ctcaggcact ctgcatcctc aaaaattttg gcctgcaaat agacctctcc tgccgattca
     5521 tattgctcgt aggctttata gacatattcc accaggcttt catcctgata atccacatgt
     5581 tccaccaggt attccatcag ctcatcaaat cctgtatttt ccgtgcaata cgggacatat
     5641 ataaaataaa attctttctt ctccagatcc tgatagacat gttgcggaaa ccacacgatg
     5701 ttactctctt ccaaaaggaa acgggacatt tcctgccgca ctctgcgcat actccacaaa
     5761 aagtccatga tccattgtct tgtaataggc ttcttctcaa aaagctgtgc aatattctgt
     5821 ctggaagtaa tatcataata gagataggcc tctccgtcta tgtatctcaa actgcagggc
     5881 agcaatcctt tgatccctcc ccggctgatc atacaatact gatatttctt ttcctccggt
     5941 ttccgttcta ataatagtct ctcataattc gcatttaagc ttctgatata ttctgtattt
     6001 agcaactatt cttccatcct ttctcactgt accgcaccac ttgtattttc cttgttatca
     6061 ctctctgttg tttcttccgt cttcccacga tacctctgac tgtttcgcag aatctccacc
     6121 ggattgttcc tcttcacctg aaatgcggct tttgcgctcc aatacctggg tacttccgct
     6181 atttcggaaa acggataagg cattcttccc gtctgttcca cggttacctg attcttttcc
     6241 agtttccaga ccggcaattc actctcccag gcaatatatt tctccgtgtc aaaaaacata
     6301 tctttctcct gcagctgtcg attcagttcc tcttttttct gcatccatct actgccgctt
     6361 ctcactgccg cccttcccat atcctgttcc aaaaggcagc gatcatattg aaaaaacagt
     6421 aaatacgtca tcaacactat cactcccaac actataggat acagaactgc cgcctccacg
     6481 gtaaaatacg ctttataatt ctttcgttgc atacgattgc tccctccaat catcctgtca
     6541 tccatgccgt tatatatgct gccagtaaaa agggaagata tgggatctgc aggctgcgat
     6601 tccgctttct gcaaaacagg aacattgcat aaagaaagat atacagaagg cttatgccta
     6661 agataagaag aatattttta tctctgctta taaaaccggt cagcgccagt acgataccat
     6721 ccgcctcacc gatttccttt gttgcctttg acaaaagcaa cagcagaatt ccgggaagta
     6781 gtgcgacaag cctgctgcat tcaaagcctt tccagacaat aaccataaca gccagtactc
     6841 cgcccaggaa caataccccc accggaaccg ctctcttccg gatatcatac cagctgcccc
     6901 atagcagata gccggtcagt atcacatttt cccacagctc catcttcacc attctcccgt
     6961 tttatattct tccgcatcta ctgcattctc tgtacccgtc cacatctttc ctggcaatgc
     7021 aatatacggt tctcttaagc ccggggcagt cccgccttct gtgataacat tcaccctccg
     7081 agcaaatata gtagccattc tcttccgggg cagcggcatc ttttttctgt accatacact
     7141 tctcacaggg atgataaccc ttaggaattt ccgctgcttc cacccgtcga accgacaatc
     7201 tcagatgtgt acattctctg cttctatgat atacttctga atcttccgtt acatagacat
     7261 attcttcatc attctcagtc cccggaatcg catagccgtt ccacaaatga ccatagtatc
     7321 ggttcgccat acggaatttt cgaaatccca aaacctccgt gagcggactg atcccatagg
     7381 tgactacaat ctcacagata tcattctttg tgctgctctc cagaaattgc aggctgtctg
     7441 ccccctgttc taatggcgac tgctccagat attcttctcc cagctgattg cagatctgat
     7501 ttttaacata ggtataggaa acagccactg ttcccaacct gtcatcgtta tcctctttct
     7561 ctccggcatc tcctgcattc agtattccgc catagattcc cacctgtctg ccggcatccc
     7621 acaatgctac ttccaggttt ccatgcaaac gcatcatttc tatagcgctt cccatatgga
     7681 tcataaaaaa acaaaacaac ggcaaaacca gtgctgtttc caccgtcata cttgccttca
     7741 ccggtgttcg cgggagtata agcagtgata tcctctttat gaagtttgct gtctgtagga
     7801 attttacggg gagtaaatcg ttacatgggt taagagtttt tcttaaagtc ggagtaggac
     7861 ttgaatcatt cttgttaata gttataaagc tgtacttgca taaagaggac atcgctgctc
     7921 acctcccttc ccggctcgtc ttcctaatta ccagtatttt ctattgcctc taaagatcct
     7981 atataagatt tctgtctctc gatctcaaaa gaatagccat atttgctttt tactttcaca
     8041 ttcattgcaa tttccacaat gcaggtatcc agtcggaatg ctctgtttcc cggagtattt
     8101 cgaatatcca tttccaccac gtccatagcc cggcaggtaa cagacttttc ctcacagagc
     8161 agcaaaaata ttctcagata atcttcatat cccattcccg gtgaatcgga accggaggca
     8221 tcctcttcgg aagtcccggg caattctccc tgtaatgcgg aagaaagtcc gtaatgccag
     8281 gtagcatctg tttttaacag gggaatcttt tccccggcaa gaagttcctt tacatcataa
     8341 acactttctg caaaagccca tcccaggatc agtgtcgccg caagcaactc tccaatctcc
     8401 ggaaccatca ttgccgctgc aagtgcctct cccaatagct ccgctattgc ccgtttttcc
     8461 tcactaccgg tcagatacat ggtatttgca gcttctctga taacgaatat tcggttcact
     8521 attcccttca gattgtcagt atcattatcc ttgcccacaa tgacatattc tgtctgatac
     8581 catagggggc tgtcctgttt ctcctggcca taccgcccaa gataccgcaa cagatactca
     8641 tggaaaaaaa ctttttgttt taacgcttcc cattcttcat aattttctgc ggttcccggc
     8701 accatatttc cgcggtttat ttctcctcgc tccatccgcg tctccaatag ggactctgtt
     8761 tctatcctgc gggaggagat attgtcttcc tctgttacca gctttaacag acctttattt
     8821 cttttctctt ccagggctgc ggtaggattc tctatatgaa cggtttttcc ttccgtggtc
     8881 tccttcccgt catattcctg aatctgtgca tcgatctgct gtttttgtcc cgcaatatcc
     8941 tgttgctcca gtccacggga ttctatgacc tgcagccagt ccgtcagttg ctgtaagagt
     9001 ccgattccca ccgtatcctc tatggcatcg acagcaaccc gccgaaagac ttctccttcg
     9061 ccatccgtca gaaatgccgc tttcacagct tgtgcttctt ctgcctgaac agcaaaaaaa
     9121 tcccggtaca acagtttttc gagaaacaca tcctccagag aaaaattacg gttcatatac
     9181 tccagcaaat gactctccgt cagctttgtc gaagcacttt ctgtcccgta agagcagtct
     9241 atggcaaaca gattgtactg tttctgcagt tctctatgat attcagccag gatactatcc
     9301 attccgatat ccatgacgca ttctgtttcc agtgagattc cccgataccg acagccctcg
     9361 atcaaagcaa gacacagggc catgatcact gtcaccgtca gtgccagata tatcgtcata
     9421 taaccacccg gggttacctt tttcacggat gtcataacgt attttcctta tatctttttg
     9481 gtctgatttg taatctttgt gaaaatggaa ttgacaactt ccgtgatctt ctctttaaaa
     9541 ataatgacca gccctaccag taccacaatg attagtatta cttccactgt acccattccg
     9601 tcttcttctg tcagaaactg tcgaaatgat tttaatttgt tgtttcccat aaccatacct
     9661 tccttttctt atattcccgc attcgaaaat gcaggcacca tgatcattac cattactacc
     9721 accagcagca tcaccatagg caggagcagt tttgtcgtcg cttcctcact gagttttctg
     9781 gcattctgta tccgcagttc ttccgcctgg acagactctt cctttaattg ctgtaaaagc
     9841 gtagaactgc cctttttcag attttgcgtc aggaatgtgc ttaaacggac atattccctg
     9901 actcctgtcc ttttcccaaa gttctcatat gctgcccctt ccggcactcc catatgtatc
     9961 tcccgggttg ctatcagcat ttcctcgtaa gcttcccggc aacgtccccc tcgttttctt
    10021 tccttctcgt aatcttctgc aacccggcaa aacgcagacc ggaccgtcaa tcccgcttcc
    10081 agatacagtg tcagcttttg caggatttcc ggatagcttc tacgttcctc ccgctttttc
    10141 ttctccacca gatcatgcag gtctttgtcc tggaagaaaa agactgccgc cgccaacaac
    10201 agagcccccg ccagaatctt ccatccagtc tccggaatat cttcttccca ggaaattttt
    10261 ctgccctcaa tttctgtggg cagctggatt ttttcacttt ccggatcttc ctcctgcgcc
    10321 cgctgcagta attccgacaa cttgcttttc tctctttcct gtggggtctc attccttttt
    10381 acgactgtta ccttaaccgt tgtctctctg acaaattccc cgtattgtat ctccgctctc
    10441 aacaatacag gcacatcctc ctgcccggga tgtatctgtc cattctcttc aacagcttcc
    10501 gtagaattgc ttttccagtt caccagaaac gggtatcctt catacgtttc cgataaatcc
    10561 agattctctc tgatctcatc gggagaatca tttttctcca ggatcaactg aggcagttct
    10621 ctcccaaact gctcgtacaa attctccaat tcgctttggc tatacttgcg aggcatcact
    10681 tccatctgga aagaatatct ctcgccgtcc tccacctgtc cggtgatgct gtattcccga
    10741 ctgccctctt ccccagatcc ccgatacacc cattgattct gcaggacatg actttcccga
    10801 ctgctgtaat gcattgtaat ggcaagcatt atccctgcga tcaatatgac tgcagaaagt
    10861 gtcagctttg tcacataata gtctgtcaca cactcctgcg gattttctcc cgggtgcata
    10921 cgattcagat ccgcttgcac cttatctctt ccaggtatcg gaattctcca caaatgtagc
    10981 tttttatagc agaccatcgt caccttatag aaaaaacgca ggatgccttt cttacctatc
    11041 atctctttct acagttcctc ctcgatccga tccagtatct tctcacccag cagatatgct
    11101 cctgcataga ctgcaaggca gagtgtcatc actatgatac ccattccatt atggtagagc
    11161 gttccaaaat atcccggata agcggctgcg atatagagag aaattccaaa cggcatgatc
    11221 tccatcacgt tctgttccag tcttctcccc tgtaacatgg tcagcatctc ctgcatggtt
    11281 tccactttat tactgatcac ctctgctgta gtccgtatga tctgcgtgac atttccaccg
    11341 cctctcttgg caatgttcaa aatagcggaa aattgctcaa tttcctcaat gccactccgg
    11401 tctgccagat cactgatcaa ctcttccagc gtgatattca gtatcattcc tctgcggatc
    11461 agttccagtt cccggtacat ggcggaatgt tcaccataca acattcgtat atcctcccgg
    11521 ctttccagaa aagcattttc caacgcatat ccgcttctca gagaatttgc cacagacaag
    11581 atgcattcct tgaattgcat ggtcagttcc tgtctgttct tccgacactg tctgttgacg
    11641 cacctgcgaa aatagagaat ccccagaggg aacaatagcg gaaccgccca gatagagcga
    11701 tagaaaaacc aggcaaaaaa tataactacc cctgatgtct gtgccagcag ccgtatgcaa
    11761 tctccccgct tctgaaaaac cttatgataa tcctgctgcc ttaagctttc cttcatgaag
    11821 taattctcct tttctccgca gcctgccgac cacttttcct tcactgtttt cccccagctc
    11881 ttcaaactga tacaaaggat tcaggcgtat ttctccattc tcacaaccta ctacctccgt
    11941 gatctccagg acacgtctac tcttatcacg gatccgcccc agatgtacaa taacatccac
    12001 tcccgaggcg atctggttgc gaatagccgt cagcggaaga tccattccca tcaggaccat
    12061 attttccagt cttgccagca tatccgtggc actgttggca tgcccggtgg acatactccc
    12121 atcatggccg gtgttcatgc attgcagcat atcgatagcc tccggtcccc ggacctctcc
    12181 tacaatgatc cggtccggcc gcatccgcag agacgaacgg atcagttccc ggattccgat
    12241 ttctctgcat ccttcggtat tgccgttacg agtctctaaa cgaaccagat tcggaagacc
    12301 ctgcagctgt agttccgcac tgtcttctat ggtgatcacc cgctcttccg ccgaaatata
    12361 gccggacaga acatttagga atgttgtctt accgcttccc gttcctccac tgacaaaaat
    12421 gttgtagccc gccttcacca gtgtctccag gaactgggct gcctccagcg aaatggtttc
    12481 cagaccgatc agcttctgca tggtgacagg cttgtccggg aatctccgga tcgtgacgat
    12541 cggaccattc agagcaatgg gattcatgac aatatttact ctggaaccat tattcaaccg
    12601 ggcatctacg atgggtgacg cttcattgac cacgcgatta caccccgcca caatctgctg
    12661 aatgacatcc tgtaattttt ccacggaatc aaaggctcga tccagttccc gcagttgtcc
    12721 atcctgttct acaaaaatgt gatccatgcc gttgatcatg atctctgtaa cagagctgtc
    12781 ctccacaaaa atctgcagaa tatccagtct tctcagggaa tcaaacaatt ccttacgcag
    12841 acgtctccgc aattctaccg ggcataactc cagactttcc tgctccatca gtacttcatc
    12901 gatcgtatcc tccacttctt catccgtata atctcttcca taatccagtc tttcctgtac
    12961 catctgacga agttttcttc tctgctctaa ataatccatt tgtccttctc ctattctact
    13021 cttccaatgt ctctatttct tttctcacat aatctgccag ttcccctctg gtatactgtt
    13081 ccatttccac gggaagtcgc tgaaagtatg gcaaattcag ctttctggtc ttccccttta
    13141 cttcctcttt ctcgcatagt gacaatagct gttcatattg atccagttta cactgtgaca
    13201 tcttgtcctc tctggtcagc gtaaatactt tgatacacat ctgcagaaca tccaacagcc
    13261 cctgtatgct ttcactcaga tccagaatga catattcgta tttccccagc tcctcgatcc
    13321 tctggaacag acttctccat tcttcccagg tgatccccaa caggttctgt ccgttccgca
    13381 tgggtggaat ataatccaga ttgccttttc tctgaatgac cgtctgcatg cgaagaggaa
    13441 attttccttc atctcctgct aaaaaataga gcagatctgc cagatcccga ttctgtctct
    13501 ccggaagcaa ttctatgatc cccacataat gttcgaaatt cagatacagc gtgggatgtt
    13561 tttctgccat cagctgtgcg aaagtcaatg cgaacgtact ttgaaggcat ctgtgaaccg
    13621 gagagtacat tccgataaaa cgggtcttat attccgcaca taacagcggc atccgtatcc
    13681 ccgtcgtctc tacatacaac gccaacagtt ccttccacac ctgtcccgct tcctgatatt
    13741 tgttaatatt gggaaactga tgaaagcgga gagttccgct ctcatttaaa attgcctgac
    13801 atgtcggctg taatgtgttc aatgcctcct cacaggagga ttccgccacc accagcattg
    13861 ttacctcccc gcttttctct cccgccaaca gttcttccgc tctggtataa gtatggatct
    13921 gccagggcaa ttccttttgc ctcttcaggt attctgaaaa aagccgcgcg tattcttcct
    13981 cccggtcata cagaaccaga accggttcct tttgttccat caatacattc ctcccatata
    14041 catcaatgca ctgcagaaca ccggtcccgc catgcaaatc cctgctccgg caccttcccg
    14101 ttctccggca aaataaagtt tccatcttcc acaccggaat acgtcccata agtactctcc
    14161 caaatagtac aaacggtctt tcatattgcg ttccagatac attttgaaaa gtgcaattac
    14221 tgctgcaatc atgagcgcga aaaacagata ataaagtact ttattccagg gaaaaaaacc
    14281 ggcacagact cccaacatct taacatcacc ggctcctatg gctccgatcc aaaaaacaaa
    14341 ataaagtaat atcgttgtca gtaatgcact tgccagatat tccaaaacgc ctttgtgccc
    14401 gccgctgatc aaccgatacc ccaggcctgc aaacatgcag catacgatca gccaattggg
    14461 tatcctgtgc gttttgtaat caaaacagca agctattgcc aaaagtatac ataatacccc
    14521 aaatatctca tctccttctc ctgatttcac attattttcc tgcgtatagt cctgtcgtca
    14581 ccttcctccc aactattcaa aatcagtctt ctggtaatgc gggatgaatc tatcccaaaa
    14641 tagaaattct aaaagatctt ataagatgct ctgaacaacc atcgcgattg gttgtaagat
    14701 gaattataaa tgctcacttt ccgtctggca ataccttttt cgtaaaaaga taaactttag
    14761 tacattttaa caaacagaat tgtttttcat attttcagtc atcctgaaat acgctatttt
    14821 acacgctttc cgtatgtcaa aacacctttt tataatattt cataaatact tagcccgtac
    14881 agcatcacaa tgcccggaat tccaaggatt cccgttgtca aaatacttac tgcattgatt
    14941 ccaaccactg aagtaaatcc aaggaaagtc acacccatat taataaaata aatggcaatg
    15001 gttcccataa tgccccgcaa tgcaaaattg aggagccact ctgccttttt ccccaaaaag
    15061 cttatgagca gcaataatgc cgccactgct atcagccata ataaattttc cggatgttcc
    15121 actgttgttc tcgcattccc gacaattctt tatcctaatc tattcctctt tccgtgtaaa
    15181 aatgtcttcc gttttcctcc atgtcaggta tatttttcct tttttgttcc tatactgtta
    15241 gcagaaagga cggttaatgt tatgaaaatc ttttttagtc aggcagatgc ctccccggaa
    15301 atcattgaag aggatttctc tcccatagat ctgaaagaat ccataagaaa aacccgccag
    15361 gcgttggaaa tcgcctatgc gggtttcgat aatgctttgg aaagtgatct cattgacagt
    15421 tatatttatg aaatcaatgc gttacaaaaa aggtatcagc atctcacaga gctggccgct
    15481 ctgcagccgg cggatcagga aaaaaatcta taccaacatt ccccgattcg tgccctggtc
    15541 agccatgttt tcggttaact ggttcctgcc gtaagtcagt ccggcatata ctgtctgaga
    15601 attgttcatc accgggccgg aagtatcctg atcctggatc tgcctgtacg catcgcagag
    15661 aggtgcgatc actttgcgaa tctcttccaa tattgtgcgg tctctgcgga cttcacatac
    15721 agcaagtctg cgaaggcaga acaggtacag tctggaaagc tctcctgccg ggctgtattc
    15781 tctgtgcaga gagttcagga gctctttgat acatcccctg gctctatgaa cagcttccgc
    15841 cagtccggca tcatctgctg cctgctctcc gtctgccaga tattgtaagg tcatttcata
    15901 caggatcacg atcaactgcg tggaattcgc ctgtgtgatc cgaagtgtaa attgttgttt
    15961 gcattctttt gtcattctgc catgtcctct tattcagaac tttcctactg taacaactgc
    16021 agtacctgag acggtctctc gttggcctgt gccagcatgg aggttcctgc ctgtgccaag
    16081 atctgataag tcgtataaga agtcatttct tccgccatat ctacatccat gatacgggaa
    16141 tatgcggaag tcatattttc gctggtaata tccagactgt tgatcgtaga ttccagacgg
    16201 ttctggaatg cacccagtct gcctcgaacg gaagatacat atttgatggc gccgtctacg
    16261 cgatcaatgg catcgtccgc accttctgca gtactgacat caacattctc gattcccata
    16321 ttctgtaaag aaaccgcagg aatatttact tccagcacct gatgttcatt agctccgatc
    16381 tgtaagcgca tagcgccgat acctgtggca ttgatcttca gatcctgaac cgttgttcct
    16441 gtctgtgctc cattcaaagt tccgctcaca tcaaggcgca tctcaaatcc gttttctccg
    16501 gtgatggtca aaagatcatc cttcagttca gtcttcagtg tcttcgcatc cgggaaacca
    16561 tctccgaacg tcacatctcc gtccagcaca atattgccgt ccgcatcctt tgtcagcggt
    16621 atagacttta tggtatattc ttttaccggc acgtcggtag aatagtagtt caccttaact
    16681 tcctgcttgt tggtatatcc cttcagatcg tactccccat tcagcagttt ctgtccgttg
    16741 aactctgtta cgtcagaaat tctggtgatt tcctctttca gttgctttac ttcatcctgt
    16801 acggtagctc ggtcatcatc cgtcattata ccgttggcag ccttgacgga gagctcattg
    16861 attcgctgca gcatctcact gatctcggtc atggcgccat ctgcaatctc gataatggat
    16921 acgccgtcgc tagcattctg gtttgccacg gacaggccct cgatctgggc attcatacgt
    16981 ttggacattg ccagtcctgc cggattatcc tttgcatgat tgatctttaa tccggaagaa
    17041 agtctctcta atgatttgga cagcaggtca tcattggtag ataaggcatt gttggataac
    17101 attgccgata cattgtaatt aattctcatg tgttgttact ccttactttc cgctgctgtc
    17161 tgcgacagcc tgcaggaatc cttttgccag
//
LOCUS       DAWDHD010000041        16575 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_233_length_16575_cov_12.900000, whole genome shotgun sequence.
ACCESSION   DAWDHD010000041 DAWDHD010000000
VERSION     DAWDHD010000041.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 16575)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 16575)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16575
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_233_length_16575_cov_12.900000"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3768..3867
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4758 a         3449 c         4687 g         3581 t
ORIGIN      
        1 ggcaacccgg cacccaccgg aacccccgaa gagatcatgg cagccggaca gaagatgtac
       61 cgtgagctgt ccccggagac tgcggaattt tttgatttca tgatggagaa ccagttattt
      121 gatgtcctgg ggcgcaagac gaagaaggcg ggaggttaca tgacctatct gcctctctac
      181 catgcaccgt ttatcttcgc taactttaac ggtaccagcg gtgatgtgga cgtcatcacg
      241 catgagtgtg gtcatgcgtt tcaggggtat ctcgctgcac aggatcctat cagagagcat
      301 gcggatatcg gcatggagac tgcagagatc cattccatgt ccatggaatt tttcacggaa
      361 aagtggatga acctgttctt cggcaacaga gcgcaggatt acgtggaaat gcatctggag
      421 gatgcggctg cgtttattcc ttacggctgt atggtggatg agttccagca tatcgtctat
      481 gagcacccgg agctgacccc ggccgagaga aagcaggcat ggagccgtct ggagcgggag
      541 tacaagcccc acctggatta cgagggagat cctttcttcg gacagggagg cttctggcag
      601 aagcagctgc acatttacga ctaccctctg tattatatcg attattgtct ggcacagacc
      661 tgcgcactac agtacaaggt gaagatggat gccgatttta cagaggcgtg gaagagctat
      721 ctgaagctgt gcaggctgtc cgccagtgat ttttacacta atatgatcaa agaagtcgga
      781 ctggacagtc cttttgagcc cggatgtgtg aaaaatatcg tggaaaaact tgaaaaatat
      841 gtgaaatagc tttctttttc cgcagaaata cggtaaaata aggagtcatt gggacttaag
      901 acaccaggaa ggagacatgt aagcaatgtc taagaatgaa aaagttacag aaaataaaga
      961 acaaacagaa cagaaggtca tgaccaaata tgaccgtaag gtacagaagc gtaaagagga
     1021 gaaggaaaag gagaaaaagg aagagcgcat cagcacggcc atcggcatcg tagtgcttgt
     1081 ggctctggta tgtctggtag catctttccc catccgtacc tatctggcaa ctcatgagac
     1141 ctatgtggtg gtcaatggag aagctgtaaa caaggtagag ttcgattacc aatacaacct
     1201 gaccaagaac aattatatca cccagtacgg cagctatctg acatacttcg gactggatac
     1261 cagcaaggat ctgtccaccc agatgtattc cgatactctg acctggcagg actattttga
     1321 gcagaatgct gtagagagcc tgaagcagaa taaggcgctg atggcagaag ccaaagcagc
     1381 cggcttcacc tacgatacca cggatgagta caacactttc aaggagacca tcaaaacctc
     1441 cgctgcatcc gcaggcatct ccgagaagga atatgtacgt tccatctacg gaagctatgc
     1501 aaccatgggc cgtatcgaag aatatgtgaa gaacgacatg gtaatgaacg cttattatca
     1561 gaagctgcag gaggataatg ctcccagtga tgatgagatc cagagctatt atgaggaaaa
     1621 taaggcaacc tacgattccg tagactatcg cctgacgacc attgaggcag aattgcctac
     1681 agaacctacc gaactggcag atcccgtaga ggagactgct gcaaccaccg ataccactgc
     1741 aacagacgga acggcagcta cagatgcaac agcttccgac agcaccgata ccgcttacca
     1801 gccttccgat gctgagatcg caaaggccat ggaagatgcc aaggtactgg cagatgatgc
     1861 ggagaagacc gtggcaaaag acggcgaagc acacgagaat gagaagaagt cttccgtgaa
     1921 ctacctgatc tccgactggc tgttcgatga tgccagaaag gcaggagaca ccaccgtcat
     1981 tactaacgac aacagccatt gctactatgc cgtggcattt gagaagagat atctggatga
     2041 gactccttcc gcagatgtac gtgtgatcat tcccaccgag gataagaccg gtgaagagat
     2101 cctggaggag tggaagagcg gtgcagctac cgaggacagc ttcgcagaat tgtgcaagaa
     2161 gtatacacag gatacttctg cagtagagaa cggcggtctg ttcgagcagg tgaccaagac
     2221 cggtatgaca gaggagctga gcaactggat cttcgacaac agccgtcagg caggagatac
     2281 cgtagccatc actgtcagtg acaccaccta tgtactttac tacatcggac aggatcagcc
     2341 cgagtggaag atcaacatca agaatacgct ggtatccgat acaatgagtc agcatatgca
     2401 ggatattacc gcagatgtaa cagtagagga tcccaagggt aaactgaatt acctgaaggt
     2461 gcaggcagag gaatccgcag ccgctgagac cgcaaccgca gagacccagg agatcaccga
     2521 ggagcagact gctgctaccg aagagaccgc aacacaggca cagtaaagct tgctcaggaa
     2581 cagaacatag acaacagaca agtacctgtt tcactgcaca agcagaagag acaggtactg
     2641 ttaggttatg agcaaaaata gtcgcaaaag ggcgggaaac atgataatgt gactctgcca
     2701 gttacgtgag ctgaacagag aataactttg agaaaggtat gaataaacat gcaggaaaaa
     2761 gttctggtac tggatattga cggaacattg accaattcaa aaaaagagat cacggaagct
     2821 accaaacagg gaatctggaa tattttacag catggtcaca aggtgatcct ggcttcgggt
     2881 cgtcccaccc ccggcatgcg gaaatgcgaa gaggagctgg aactgaaaaa atacggcgga
     2941 tacctactgt catttaacgg agcccgtatc gtggagtgta agacaggaga agtggtctat
     3001 cagagaacac tgcccctgtc cattgtgccc ggattgtacg aattcgccaa agcgaatggc
     3061 tgcggactga tcacttatca gggtcccggc gtcatctctg cgttcgagcc ggatgactat
     3121 gtgcagctgg aagccagaat caacggaatt cctataaagg tcgttgacaa tttcgtcgaa
     3181 tatgtgaatt tcgatattaa taaatgcctg atgacagcac cggaagagat cgcaccggag
     3241 ctggagaaac agctgcagga aaaatacagc gatgtggcaa gcatctaccg ttccgaaccc
     3301 tatttcatcg agatcatgcc gaaagatgtg aacaaagcca cctctttgga tcatatgctg
     3361 gaaggcatgg gactgaccag agagaatgcg atctgctgcg gggacggtta caacgacatc
     3421 tccatgatcg aatatgcggg aaccggcgta gcaatgggga atgcccagcc ggcggtcaag
     3481 gatgcggcgg actatatcac cggcaccaac gatgaagacg gactggtgga agtcatcgat
     3541 aagttcatca tgaacccgga gcaggcatag cctttcttag aaacaaacaa tagacacaaa
     3601 gcagtcacaa gcgaaaactc acagagcaat gagcgtgtgt gatttttgca aaagaggata
     3661 tatatgattc agatacaaat acctgaaaaa gcgaaatata tcatagaaac catacagaat
     3721 gccggatttg aagcctatgt ggtaggcggc tgcgtgcggg attccatnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnntac agaatgccgg atttgaagcc tatgtggtag
     3901 gcggctgcgt gcgggattcc attctgggga gaaccccgga ggactgggat atcaccacct
     3961 cggcgagacc ggagcaggta aagcagctgt ttcgtcgcac catcgacacc ggaatccagc
     4021 atggcaccgt aaccgtcatg ctggacaagg aaggctttga ggttacgacc taccgtgtag
     4081 atggaaaata cgaagacagc cgacatccca aagaagtgac gtttactccg aacctggagg
     4141 aggacctgaa acgccgggat tttaccatca atgccatggc ttataatgaa acggaaggcc
     4201 tgatcgacat tttcggcgga ttaaaagaca tcgaggcgaa gctgatccga tgcgtaggca
     4261 acccggagga acgttttgga gaagatgcgc tgcggatcat gcgggcaatt cgtttttcag
     4321 cccagctggg ctacgagatc cacgaagata ctgaggcggc aatccggaag ctggcaccga
     4381 ccctgcagaa gatcagtgcg gagcggattc aggtggaact gactaaactg ttggtgtccc
     4441 ctcatcccga caccctcaga gaagcttatg acatgggtgt caccaaggtg atcctaccgg
     4501 aattcgatgc catgatggag acaccgcaga agcataagca ccataaatac aacgtagggg
     4561 agcatacatt acatgcactt atggagatcg ggccggacaa gaatctgcgt tatgccatgc
     4621 tgctccatga catcggcaag ccggccacac ttactgtgga tgaagacgga atcactcatt
     4681 tctacggaca tccggcggtg ggagaagaga tggcgcggaa gattctgcgg agactgcgat
     4741 ttgataatga taccgttgcg gtggtcaccc gtctggtgag ataccatgat tatggcaatg
     4801 atgtgatacc ggatctgcgg attgtccgcc gggcggtgaa taagatcggg gaagatattt
     4861 tcccactgtt gttcccggtg aaacgggcag atattctggc acagagcgat tacctgagaa
     4921 cagaaaaact ggagaatctc gaactctgga agaaactgta tcaggaaatg ctggaaaaga
     4981 agcagtgcgt ctccctgaaa acactggcgg tcaccggcag ggatctgatc gacaccggta
     5041 tgaaaccggg cagagagctg ggagagatgc tgcaacagct gctggaactg gtattggaac
     5101 atcccgagca gaataccaga gaacagctgc tggaaaaggt aaaggaactg tcggcagaca
     5161 aagattgagc atactttaca ggctttcctc ataagatagc gtaggaaccg gaaaacggtt
     5221 aaggacgtat cttaggaggg attgtaagtg aatgacagag cagtggcact gctggagcag
     5281 tatgagatag aagtattgcg tacccgaaaa ggaaggggag cgattctgtg cgatacacag
     5341 cagggctgcc tgatcttcaa ggaatatacc ggaagagagg aacaattatg cctgcaggag
     5401 caggtcctga agcacctgga ggaacgggga accgttcctg cggagcgatt gcttgccaca
     5461 aaggaaggcg cgttatcggt gacagaccgg gacggtgtca gatatattct gaagacctgg
     5521 tgggaaggcc gagaatgtaa tatccgtgac agggaagaat gtatggaagc catgcggctt
     5581 ctggcgagac tgcacggtga tctggaattt acaccggtat ttccggagac ggaggagact
     5641 tcggcaccgg tcatagagcg atatctcccc agccgggact atgagaagca taaccgggag
     5701 ctcaagagag cccgcaggtt cttgaaacag cgcagccaga agacctggtt cgagatccgg
     5761 ttgtctgcag tgattgatcc ttttttagag gaagcaagac aactgtgtga agaatggaaa
     5821 gagatcgagc tggctgcctc cgacagcgga gaggaagtac cgctgtgttt ctgccacgga
     5881 gattaccaat atcataatat tctgcggcag gaccgtggat ttttcctggt gaacttcgaa
     5941 aaatgtcagg cagacgggcc ggtgagagac ctgtatctgc tgttgcgaaa gcttttggaa
     6001 aaaagtgaat gggatgccga atggggcaga gtgcttctgg cggcttatga aagtgtcaga
     6061 ccgttaaagc cctatgagag acaggatctg gtctaccgcc tgtcctatcc tgagaaattg
     6121 tggaagatcg taaactttta ttataattcc ggcaaggcct ggatcccgga gaagaatcag
     6181 gagaaactgg accgcctgtt ggaacaggaa gcggccagaa agaaattcct gaaactgctg
     6241 caaagataaa agccgggcaa tgaacggcag acaacggaag agacgttgcg gcaggatcgc
     6301 agcgtcagga gacaaaggag aagcaaaaac gttgaaaaga ttcgaagaca aaaagtattc
     6361 gaaaacgaaa cagacagtgg taattctttt gcttgtgaca gcaatgtcat ttgctggatg
     6421 cgggcagaca gcatccttcg ggacggcggc aacacctgcg ccaacggcac aggcagagcg
     6481 gatagatacc ggctttgttg tcaccaatcc ggacagctat gattccgcag atacggcggt
     6541 actggtggat atcaataatg ttgacaacac tgtcacactg ttaaatctgg atctgggcaa
     6601 acgctacacg ctgtccatgg acggaacaac gcggttcgca gataaatatg gaaaggcaat
     6661 ttccctgagt cagctgcagg agggggacat cgtggatgtg acttttttga aaagtcagaa
     6721 gcatctgacc tccatgcagc tggcgcagac agcatggcag tatgaagatg tggagcacta
     6781 cgagatgaat accgtacgga atgaggtggc catcggggaa gaggtgttcc agctgtctaa
     6841 ggatacccag tatttttccc agggacacag cattgaaaaa atggatctga atccggcgga
     6901 tatcctgaca tttcacggca ttgacagcac agtgctcagc gtaacggtgg aaaaaggcca
     6961 tggatacctc agactggtca atgatgagaa ctttgtgggc ggctggctgg aaatcggcca
     7021 gacacagatc gtacggatca cagaggacat gctggtgact gtgccggagg gaagctatca
     7081 ggtagatatc agctataacg gaggtggcgg cacgaaaaat gtcgtaatca accgcaacga
     7141 ggaaacaaca ctggatatcg gtgatctgga agtggcagag gcacagtacg gtatggtgct
     7201 gttttccctg aatccttccg atgcagaatt gtatatcgat ggaagtcagg tggatgcttc
     7261 ccagccggtc acgctggaat acggtattca tcagatcata gcgaaggcgg acggctataa
     7321 gtcgcttaca cagtatctca gggtggggca ggaatccgcg ggagtggatg tccagctgga
     7381 taaggcggac tccgacagtg aagacagtac ggaaagcagc agttcttcca gctcttccgc
     7441 aacgggatct acctccaccg cagatacgac tacgacctat taccgcgtgc atattgatgc
     7501 tccggaaaat gtggaggtat atctggacgg caactatgtg ggaatttccc cctgcagttt
     7561 taagaagacc tcaggaagtc atgtgatcac cctgcgaaaa tccggttatg aaaccagaag
     7621 ttatacggta caggtggatg aagaagaaaa ggatgtatcc tactcatttg tagacctgac
     7681 ggccagctct tcatcgacag aatgatcctt tccgggaggg gaagaaatgc cgtaaatatg
     7741 cggaaaaacc ttgacaaaag ggataaaaac agatatatat atgtatagac gtttcaaaag
     7801 aactattttc gagcgtaaaa ttaataacga aaactcatta caggaggagt tcaatatgaa
     7861 caaaacagaa ttagttgcag ctatggctga tcaggcaggt ctttccaaga aagacgcaga
     7921 gaaggcatta aaggcattca ccgatgttgt tgctgaagag ttaaagaagg atggtaaggt
     7981 tcagttagtt ggcttcggta ctttcgaagt atccagcaga gcagctagag agggtagaaa
     8041 tcctcagagc ggcgcaccta tgaagatcgc tgcttccaag gctcctaagt tcaaggctgg
     8101 taaggcatta aaggatatgc tgaacgcata atagaagctg aattttcaga gactcgatag
     8161 gacaaccttt cgggtctctt ttgtttttca gaaacggatg atcgtgaggt taaaagcatg
     8221 agattagaca aatatttgaa ggtatcccga ctgatcaagc gccgtactgt ggctaatgaa
     8281 gcctgcgaca gcggcagagt gctgatcaat gataagccgg caaaggcatc cgccaatgtc
     8341 aaagagggag atatcatttc catctctttc ggcaacaagg aagtaaaagt cgaggtcctg
     8401 gatgtacagg agaccgtgaa aaaagaagaa gccaaagaac ttttccgtta tatataatat
     8461 caggttctct atgtataaat ttccacattt tcccatacaa tacattgtaa gtccggttcc
     8521 ggaaggagcg gaggatggga ggctatggag agaatggaag atttaaacgc gacaggacgt
     8581 atgcataaag tgaccatgac caacagacgg acatgtaccg tgaatggggt aaatgacgtc
     8641 ctttcctttg atctgcggga gatcctgctg gagacggaac aggggatgct gatgatccgt
     8701 ggcaacgagc tccatgtgaa ccgcctgaca ttagacaagg gggaagtgga tattgacggc
     8761 cggatcgaca gccttgtata ttcggatacc gcagcatccg gagaaaaaca ggaatctttc
     8821 ctgggcaggc tgttccgctg atggatgaaa atgtttttct gctgcattcc tttctgctgg
     8881 gtgtggggat cacttttctg tatgatattc tgcggatcct gcgaagagtg ttcgctcaca
     8941 gtaccagcat ggtatctctg gaagatctgg cattctggat ctactgtgcg ggaaaggtct
     9001 ttctgctgat gtttcatgaa agtaacggga acctgcgatg gtttgccgtg ctgggggcat
     9061 tggcgggaat gtacctgtat cacaggacaa tcagtccctt ttttgtaaag tatgtggcac
     9121 tgggcctgaa tactcttctg cacccaatcc ggaaagcctt gcggataacg ggaaattggc
     9181 tgaaaaagca gttgactata cttaggaaaa tgcttaaaat attcattaaa aggagatgcg
     9241 cgtatggcaa gaagaaaagc agcctaccgc aagagacacc agaaccggtt cagtatgttc
     9301 ctggtgaccc tggtcgtgtt gatgatcatg gtggtcgttg cggtgcggag cgtggaatta
     9361 caacagaaga ttgcgggcta tgatacccag attgcaagcc ttggcgcgca gatcgaagca
     9421 gagcagacca gagcggaaca gatcgaggaa ttcggtaagt acactcagac gaaagcttat
     9481 gtggaagagg tggcgaagga taagctggga ctggtatacg aaggtgagat cctttttcag
     9541 gaagacaatt aagcagggaa actgtttctc aacagtgaac ctgctttttt tgtcgcaaaa
     9601 aatgaagtct ctccctccct cgatttgaca aaaggaagag gaaaactgcc ctataatcgt
     9661 gaaaacagaa aggggaggat gccatgatca tagggaaaaa ggagcaggaa cggctggaga
     9721 gagaacagtg tgcacagatc tcactgctgt gcagacagaa actgatggga tatgcggaga
     9781 gttttgagga actggggaag agctttcggc aggagataca gattacagga gaggacaggc
     9841 agaatattct ggaaaaatgt atgctgtggc agagcagaca gatcatggga gaccatctgc
     9901 aggaagtggc ccggatcatg gaacgggtgg caggagagga actggggtat tgtccgctgg
     9961 aagagaaaaa gaggaaaagc ctggtacagg cgctcaagag tgaagggatc atcgccagag
    10021 acctctgcta tgtggagaag gacggcagca gctccgggac agaagcaggg atcagtatga
    10081 cactgtccac cgaaaaaagg cactataagg cggcacaggt ggcggacatg ctgacggtat
    10141 tattgggaaa aaagctgcag gtctcttcag gaagccctta tctcgtggag caggagccgc
    10201 attgttttct gttcacagag gaaccgaaat acatagcact gacgggggtg agccgggcgg
    10261 taaaagaagg ggagaaagtc tcgggggatc agtatgccac catggaatcg gaaaaaggga
    10321 agctgatcct gctgttgtct gatgggacag gctccgggga ggatgccagc cggggcagcg
    10381 gcagagtcat ggatctcatg gaaaaaatgc tggaggcggg cttcgggacc gaggcatctg
    10441 tgaatctcct gaattccgcg ttgtatgcgc aaaatgaaga ggacgatcat cctacgatag
    10501 acctttgcag cctggatctg tataccggcg aatgtgagat ctgcaaggtg ggcggagttg
    10561 ccacgttctg gaagacctcc ggaagggttc tctgtgtcgg cggcgaaagc ctgccgttag
    10621 gtatttttca aaaagtgcag atagaacgac agatgtgtca gatccggccg ggagatctgc
    10681 tggtcatgat gacggacggg gttctggata tgatggaaga cggggaagag cgcatggcac
    10741 aggtgctgga agggctgcag gaacagaacc cgcaggagat cgcagagaaa atattgtcct
    10801 atgccatctg tgcctcagag ggacggattc gggacgatat gacggtattt gtgctctgcc
    10861 tgtgggaaaa cacgtgatgc cgccttgatt tttccccata attaggatag agtaaaaaca
    10921 tgaatacatg gatggatacg cgagaaaaag tatttgcata tatcagagag catcacatgc
    10981 tgcaggccgg cgaccgtgtg gtagccggtg tttctggagg ggcagactcg gtttgcctct
    11041 tatttttgtt gctggaatgg cagaaggaag tacctacaga cattgccgta gtgcatgtgg
    11101 atcacggcat tcgcgcggag gccggagaag atgcccggta tgtggagcag ctctgcgagg
    11161 agagaggaat cccttttttc ctgaccaggg cagaggtaag gaacagagcc cgcatggaga
    11221 aaatctcgga ggaagaagcc gggcgcagaa cgagatatga ggcgtttgaa aaggcggcaa
    11281 aggagtgggg agccacgaag atcgcagtgg cccacaacag caatgaccgc agcgagaccc
    11341 agctgttcca tttgttccgg ggcagtggaa tccggggact ggccagcatt ttgcctgtga
    11401 gagatcggat tatacgaccg ctcctatgtc tggaacggtg ggagatcgag aagtttttac
    11461 aacagcgagg aattgtctat tgcaaggatg ccaccaatga ggaggatgac tacacaagaa
    11521 atcgtattcg gcatcatatt ttgccttatg cggaggagaa tattgtaaag ggctgtgtag
    11581 cgcatatgaa tcagacggcg gagctgctgg cggagacgga ggactatctg gaactgcaga
    11641 caaaagaagc agctggccga tgtatccggg caacggacgg tgacggagag tccgcagaga
    11701 cgggggacag ccggaacgcg gtgatctgta ttgctgtgga gtcttttctg tcgttgcatc
    11761 ctgtgatccg taagcgcctc gtgtatgaag aagtgaagca attgtctccc gggcagaaag
    11821 acatcactta ccagcatatt caggagctgc tgaccttgtt taccagggaa ggcaaccggc
    11881 agatctgtct tccgttcggc atccggggta gacggcaata tgaggaagtc ctgctgacag
    11941 tagagaggac agacagggag cttttggaga agacggaagt ggctgagaaa tatcaaccaa
    12001 ctctgcagct ttctcccgga cagacaggga cagtggaatg tgaagtggga acggtggaat
    12061 actgtgtgtg tgacctggcc gctgcccatg aaaaaaacca agaagttccc acaaatcagt
    12121 atacgaaatg gtttgattat gataaaataa aaaaatgtcc ggtgatacgg acaagaaagc
    12181 cgggtgatta tctgaccatt gctgacggca gaggcgggat gatccataaa tcgctgaaaa
    12241 gttatctggt cgatcaaaag atcccacagg cggaacgcga cagactgccg atcctggcag
    12301 agggagagca tgtactgtgg gtcgtgggat accgtatcag cgaatattat aaagtaacgg
    12361 agaatacaaa acgtatttta aaggtacaat taataagaaa ggagttcgct taaaagcgaa
    12421 aggacaaaag aaaatggcag agcatatcag agtactgttg acagaagagg aagtagataa
    12481 gagaatccag gagatcggtg agcagatcag caaggattat gcgggtaagc aggtacacct
    12541 tgtatgtgta ctgaagggtg gcagcttttt catgtgtgaa ctggcaaagc gtatcactgt
    12601 tcccgtatct ctggacttta tgtccgtatc cagctatggc agcgatacca agtccagcgg
    12661 tgtagtcaag atcgtgaagg atctggacga atctctgaaa gataaggaag ttattgtagt
    12721 agaggatatc gtggacagcg gaagaaccct gagctatctg ctggagatgc tgaaggacag
    12781 aggtccggct tctttacgtc tgtgcaccct gttggacaaa cccgagagaa gagtgatcga
    12841 tgtccatgtg gattataccg gatttaatat tcccgatgag tttgtagtgg gctatggtct
    12901 ggattatgat cagagatatc gtaatctgcc ttacatcggt gtggtagaat tcgattaagc
    12961 gatacaaaga tgtaggaggc ccgtgaagtt gaaacaaaac ggcaaaacaa tcaattcata
    13021 ttttatattt atcatacttc tgcttctggt gatcttcggc ctcaacctgt tgaacaaccg
    13081 tacagatgaa tacaccaagg cagagttcat tgcggatctg gatgccggca atgtatcgga
    13141 agtcgtggtg cagcccaacg gagaagcacc taccggatat ctggagatcc agatgaagaa
    13201 cggtgtatct cataagctgt acgcaacgga cattacagaa ctggagaccc tggtacgtga
    13261 gtatgggttt gatccggtgg tcaatgacat tgaaagggag aactggttcc tgacctatat
    13321 gctgccgatg ctggtcgtgc tggcggtggg aattttcctg ttcatgatga tgaatgccca
    13381 acaggcaggc ggcggtaacg gcaagatgat gaacttcggc aaaagccgcg ccaagatgac
    13441 attgggtgac aagagtgtca cattcgctca ggtagccggt ctgaaagagg aaaaggaaga
    13501 actggaggag atcgttgatt tcctgaagga gccgggaaaa tacaccggtg tcggagccag
    13561 aatccccaag ggtgtcctgt tggagggccc tcccggaacc ggtaagaccc tgttggcgaa
    13621 agccgtggca ggcgaagccg gagtaccttt tttcagcatc tccggatctg atttcgtgga
    13681 aatgttcgtc ggtgtgggcg cttcccgtgt gcgtgacctg tttgaagaag caaagcataa
    13741 tgcaccttgt atcgtattta tcgatgagat cgatgccgtg gcaagacggc gtggtaccgg
    13801 tatgggcgga ggacacgatg agagagagca gaccctgaac cagttactgg tagaaatgga
    13861 cggtttcggc gtcaatgagg gcattatcgt tatggcggcc accaaccgtg tggatattct
    13921 ggatcctgca atcctgcgtc ccggtcgttt cgaccgtaag gtagcggtgg gaaccccgga
    13981 tatcggtggc agagaagaga ttttgaaagt acatgcgaag aacaaacctc tgggggatga
    14041 tgtgaatctg cagcagatcg ctcagactac tgcaggattc accggagcgg atctggagaa
    14101 cctgctgaac gaagcggcca ttatcgcggc gaaggagaac cgctccttta tcgcacagaa
    14161 ggatatcaag agagccttta tcaaagtggg aatcggtgcg gagaagaaga gccgtatcat
    14221 ttccgacaag gagaagaaga tcactgcata ccatgaagca ggccatgcga tcctgttcca
    14281 tgtacttccg gatgtgggac cggtctatac cgtatccatt atcccgaccg gagtgggagc
    14341 tgccggttat accatgcccc tgccggaaaa ggatgagatg ttcgccacca agagccgtat
    14401 gctccaggat atcatggtat ctctgggagg acgtattgcg gaggagatca tcttcggtga
    14461 tatcaccaca ggagcttcca gtgatattaa gaaagccacc aagacagccc gccgtatggt
    14521 gacccgttac ggaatgtctg ataatatcgg tgtcatctgc tatgacgatg acgatgatga
    14581 agtcttcatc ggtaaggatc tggcacatgc caaggcgcac agtgaggaga tttccggtga
    14641 gatcgataag gaagtcaagc acatcataga tgactgctat accaaagcca aggatattat
    14701 cttacagcat gaggatgtgc tgcatagctg tgcacagctg ctgttggaga aggagaagat
    14761 caccagagaa gaattcgagg gtcttttcgt ataggaaaag gttttcgcgg gagtttcgtg
    14821 gaattggtag tattgtacaa accgattttg tgaaaaatat acaaaaatga atattcactt
    14881 ttgtgatatt tgtgaatttt gagaatagtc aaaaatagga gtgcatgcta ttataatact
    14941 cgtaaccccc ccaatacatt atatagtttt ttgctatacc ccataagaaa gaaatacctt
    15001 ctctaaaaag ggcagtcctc ccaagactgt cctttttact gcctaaaaat cgctgaataa
    15061 agaggcgggc agttgtcaga tgtaaggaaa aagggtataa taaatagagc ttttgagctt
    15121 gggacatgtc atactgccgg agcgcacaag acaacaaagc actttggcag aagcagtgtc
    15181 ataatataga tagtaagaca ttagtctcag aaagggtaga tacgatggat ctggagcgat
    15241 tacgtcgaac agaatataat cagcaataca ttgcggccat gagacctgta tttaacagga
    15301 gagcactttt tacagatacc agtgcggaat atgtgattcc ggaagagccg gcctgttttt
    15361 ccgaagtgac gatccgcttc cggaccgcca gaaataatgt ggacagagtg ttcctggtat
    15421 gcggcggaca gaagcatctt atggtacgtg tggagagcaa aaatgatttt gactattatg
    15481 cctacgtaat gcgtctggat gatcagaagg tatcttatta ttttgaggtg cagaccggca
    15541 gaattaccgg catctttgat atgcggggcc tggtgcagga agttaatgaa tattatgatt
    15601 tcattatcat tcccggtttc cacacccccg actgggccaa gggagcggtt atgtaccaga
    15661 tctatacgga tcgtttctgc aatggagatt cttccaatga tgtgttgacc aatgaatatt
    15721 gctacatcgg agaaccggta caccgcgtag aggactgggg acgttatccc gcacagatgg
    15781 atgtccgtga gttctacggc ggagacctgc agggtgtgtt ggataagatg gactacctgc
    15841 aggatctggg agttgaggtg atttatttta atcctctgtt tgtctcccct tccaaccaca
    15901 aatacgatat tcaggattat gattatattg atccccatat tggaaagatt gtcagtgatg
    15961 aaggggatct gcttcccgac ggacagcggg agaaccgttt cgcttcccgt tacatcgacc
    16021 gtgtgactaa caaggcgaat ctggaagcca gcaacgagct gtttgctcag gtggtggcag
    16081 aggcacaccg cagaggtatg agagtcattc tggatggcgt gttcaatcac tgtggttcct
    16141 ttaataaatg gatggacaga gagcgcatct atgagaatgc ggaaggatat gacaagggtg
    16201 cctatgtcag tgctgacagc ccttatcgga attactttga tttccacaat caggcagcat
    16261 ggccctataa caattcctat gacggatggt ggggacatga cacactgccg aagctgaact
    16321 acgagggatc ccaggaactg atggactatg tccttcatgt agcgaaaaaa tgggtatctc
    16381 cgccctacaa tgtggatgga tggagactgg atgtggcagc tgatctgggt cacagtcagg
    16441 aattcaacca tcatttctgg caggaattcc ggaaagcggt aaaagaggcc aatccggagg
    16501 ctattattct ggcagagcac tacggcaata ccagagactg gctgcagggc aacgaatggg
    16561 atactgtcat gaact
//
LOCUS       DAWDHD010000042        16353 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_237_length_16353_cov_10.618113, whole genome shotgun sequence.
ACCESSION   DAWDHD010000042 DAWDHD010000000
VERSION     DAWDHD010000042.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 16353)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 16353)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16353
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_237_length_16353_cov_10.618113"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         4112 a         4312 c         3181 g         4748 t
ORIGIN      
        1 attcacccag ataagccttg atgacttccg gattgttcag cacatcactg gtcttgccct
       61 gacacaggac acggccaaag ttcagaaccg taagttcctc acagattccg cctaccagtt
      121 tcatatcatg ctcgatcaga aggatcgtca tatcaaaatg ctctctgaca aaacggatgg
      181 tctccatcag ttccagcgtc tcgttagggt tcatacctgc agcaggctca tctaacagaa
      241 ggagcttggg cttggtagcc agtgctctcg caatctccaa ctttctctgc ttaccatagg
      301 gaagattgtc cgccagtgtt tccgcttcct cttccaatcc gaataccttc agaagctcca
      361 tggcctcctc catcatccgc ttttctactt tataatattt cggcagacgt aaaataccgg
      421 taaccgtgct gtagctcata tgattatgca gacctacttt tacattgtct atcacagaca
      481 gatccttgaa cagtcgaata ttctggaagg ttcttgcaat tcctgcctga ttgatttcga
      541 tggtcttttt accggtaaca tcctttccat ccagcgtaat aataccctct gtgggcttat
      601 atacacctgt caaaaggttg aatacggtgg tcttacctgc accgttggga ccgatcagtc
      661 cgtacaggga acctttttca atcttaatat tgaaatcatc taccgctcga tgtccgccga
      721 aagatatgcc taattttttt gcctctacta atgccaggct gattcaccct ccattttcac
      781 cgacttattg aatctggctg ccactttttc tctccattca atacacttcg gagcccagtt
      841 gaacagcatc attacgatca gtacgatgga atagatcagc atacgataat cgttcagacc
      901 acgcagtaac tccggtaaca gggtaaggat tacagaagca ataatggaac cgcgaatatt
      961 accgatgcca cccagtacaa cgaataccag tatcataata gacatgttat atccaaagtt
     1021 cttgggtaat gccgtcagtg tggacagatt atgcgcatac agtacgcctg ctacacctgc
     1081 aagtgccgca gaaatggaga atgccatcag tttatacttt gtaatatgaa ttcctacgga
     1141 ttccgccgca atggtattgt cacgaattgc catgatggca cgtccgtctc tggaatggat
     1201 caggttcaat acaataaaca atgtgatcag caataacaca ataccaatgg taaagctgga
     1261 atcatgaggg gttccggtga taccctgtgc acctttgata ataacgtctc cgtcctctga
     1321 catgttcaga tccatggtat tggtaaagga gatatggaat ccattctcat cccggcctac
     1381 aaacataata tttaccagat tcttaatgat ttcgccaaaa gccagtgtta cgattgccag
     1441 atagtcaccc ttcaaacgaa gtacggggat accgatcagc agtccgaaca cacctgcaaa
     1501 aaatgcaccg atcagaatag caaagaaaaa gcgaagtccg gaaggcattg cgctattctc
     1561 cattactttg gagaaaaatg cgctggtgaa tgctccgata cacataaatc ctgcatgtcc
     1621 aaggctcagt tctcccagaa tacctacggt aaggttcaga gataccgcca gaattgcata
     1681 agtacacagc ggaaccaata gtccctggat caagctgctc acactgccgg cagacaccag
     1741 aatctgcatg atgacataag ctgcaatcac cattccgtaa gtaataagat tatcccggaa
     1801 ggttttattt attttcttat tcgataattt cttcatatta tgtacctgcc tcttccctta
     1861 tactttttcc tgaatattct tgccaaacag tccggcaggt tttaccagaa gcacaacgat
     1921 caaaactgca aatacaatag catcagccat ctgggaagaa atataggctt taccaaagat
     1981 ttcgataatc cccaacaata taccaccaac cagagcaccg gggatagatc cgattccgcc
     2041 aaatacagcc gccacgaaag ctttaatacc gggcatggaa ccggtgtacg gtgtcaggga
     2101 aggatatgcg gaacataaga gaactcccgc tacagcggcc agtccggatc cgatggcaaa
     2161 agtcagtgca atggttccgt ccacattgat acccatcagc tgtgcagccc ccttatcctc
     2221 ggatactgct aacattgcct gtcctgcttt ggtcttgttg ataaaaagca tcagacctgc
     2281 cataatgatc agacaggtaa caatcgtaac gatggtctct ccggtaatat tgatctgtcc
     2341 gtctgccagc ttcacagcgg gaacagatac tacagaagta aatgccttgg catttgcacc
     2401 aaagatcaaa agagctacat tctgcagcag atagctgaca ccgattgcgg tgatcagaac
     2461 cgctaacgga gaagaagcct ttcttaaagg cttgtatgct actttttcaa tagccattcc
     2521 caacagggtg caggcgataa cagctgccag aacaccgatc acaggagaaa gtcccatggt
     2581 actgcacatt gtataagtaa taaatgcacc gaccataatg acatcaccat gtgcgaaatt
     2641 cagcattttg gcaataccat ataccatggt ataacctaat gcgatcagcg catagacact
     2701 tccaaggctg ataccgttga tcagataact gataaaactc atgtgaacta cctccattca
     2761 tgcgaatgca caaattatca tataaaataa agtataacag aacataccgg gaaaaacaac
     2821 tgctctccca ttattgcaaa gtctctgaca ttaagcaaag agggctaccc tgcggtaacc
     2881 ctctttggaa actttatcag caaaattaca tagccttgta agcaccattc tcgatcacaa
     2941 cagccttagg agccttgtta gcctcaccgt ctgcggtcca ggtcatgccg ctaccggtca
     3001 gaccatctac agtgatctct gtcatggcag ccttcatacc atcacaaata tcagaaacac
     3061 tcatatcagg agtaacaccg ctcttctcga tagcagcctt gatcacatag atagcatcat
     3121 agctgtctgc tgcgaactgg ttgggagtct caccgtattt ttccttatac ttagttacaa
     3181 aagactgggt cagatcatcg gttgcatcag ctgcgaaagg agttaacagc ataacgcctt
     3241 cggtcagaga tacatcaaaa ttatctacac caccgagaat accatccata ccatcacatg
     3301 agaaccactt gggagcataa cccatagcag ctgcctgtgt aaggataagt gcggattcct
     3361 gataataaat aggaaggaat accatgtctg ctccggcatc ctttgctttc tgtaactgta
     3421 cggagaaatc cgtcttgtta tcagcagtaa atgcttccgc agctactact tcaaagggct
     3481 ggttttcagc ttcctttaca aacttctcat aaataccgga agaatataca tcggagctgt
     3541 cataaataac agcaatcttc tcagccagac cattctcacc aatatactgt gcggatgcaa
     3601 gaccctggtt aggatcggag aagcatacgc ggaagttgtt atcatactga acacattcta
     3661 ctgcagatcc ggaaggagtg atctggaaca tattgtcttc tttggtcttg tcggtaaccg
     3721 caatacatgc tccactggtg gtagatccgt cgaggacctg tgcgccccag tcctttaatg
     3781 cattataagc atttacggat ttctcagcat ctgcctcgtc atcctggaac ttgaactcta
     3841 cctgatagcc gttgataccc cctgctgcat tgatctcttc tactgcgatt tctgcaccat
     3901 gctgtacagc cagtccgtaa actgctgcgc caccggtggt aggtccgata ccgccaattt
     3961 tgaatacact gccggatgct gcaccgttgt cagctgctgc gctctcagtt gctgcgttag
     4021 taccggttgc agcggaacca ttgtctgtat tgccgcagcc agccatagcc ataaccatgg
     4081 cagatgctac aacaacactt aacaccttgt tgaatttctt cataatatct tcctcctttt
     4141 taacaatgta tacaaatata caatgtataa ttgttatttt tgtatattac tcgcttctat
     4201 ttcctttgtc aatggacttt attttctaat ttgatgtttt ttgacattaa aatagatcaa
     4261 caaagcttaa tttttattta aaagtagtat attttttatt tttgggggta agactatcta
     4321 tgaaacaatc attaaataga aacttcaaaa aatgtctgta tggattttgc agcagcaaat
     4381 ttcatacaga caggataagg agattttatg ggtaataaat atttggacgg aaccgctctc
     4441 accatagcta taataggtgc agtgaactgg gggctgatcg ggcttttccg ctttgatctt
     4501 gtagccttcc tgttcggtga catgtcctgg ttttccagaa ttgtatatgt tttggtggga
     4561 atcagcggac tctatctcat cagcttcttt ctccatctgg gagacagcga agcttaaagt
     4621 tcctctacgg gaatctgtac gccatctctc cagatacgtt ccagatcata gagcagacgg
     4681 ttttccttgt caaagacatg tacgatcaca tcgccaaagt ctaacagtac ccagttggct
     4741 gtctcatagc cttcggtctg tctggcaggg aaaccagctc ttcccagttt ctcttccaca
     4801 ttatcacaca acgcctgaat ctgactggaa ttattaccgc cggcaataat aaaataatct
     4861 gcgatcacag atactttact gatgtctatt actttaatat cttctgcttt tttatcatcc
     4921 aatgcttcta cggcaagctt tgccattttt ctggattctt cgctcatgct aactcctcac
     4981 actttctatt ttatttatcc attcttctta taatagttat aggtttcttc tgtcatggga
     5041 tccatctcac cgtcccccga tttcagatat tccagcgtat cctctaaaat cttcgtcacg
     5101 gcagcatcca gatccgtaaa tgccaggtgg cgtatctggg taaggttcgg tgcctgtttt
     5161 ctccccggtt caatataatc tgcggtaaaa atgatcttct ccagaagtgt catacccgga
     5221 cgccctgtgg tatgatataa aattgcactt aagatctcag gatcttcgat tttatattcc
     5281 tttttcgcca gatagcttcc cactctggca tgtaaaagat agggattgcg cgtttccaca
     5341 tccgtcgccg gaagatgata cttttcgcag acagacaaca gtttatcatt ttccatgcat
     5401 ttggcacaat catgaagcag tcccgcgatc tgtgcacgct ccacatcttc actatatcgc
     5461 atggcaaggg ccgccgctgt gtattctacc cccaatgtat gggtaaagcg cttgggatcc
     5521 tgtacttttt gcatctcttt ggtcagtttt tttaagatat cactcatcca ggtaaagccc
     5581 cttttcttcc atatattccc tcactgtttc cggcacaaga tagcgcacgg aaagaccttc
     5641 ggatcttctc gctcggattt ccgaagaaga gatctcaatt cttcgaaaag agagcaactg
     5701 tatcttcgca gaaaaatgtt cctccaactg actgatcttc tcctgcattc ccttcatatc
     5761 cgcatctccc cgtaccgcaa ctataatctc gcaggcagca aacaggcggc ctgcgcattt
     5821 ccattgttcc atggtaaaca ggcagtcagc cccacagatg aaataaaaaa ggtgctccgg
     5881 atattctctg tgcagctgct ccatggtctc atagctatag gaatttccgg gacgttttat
     5941 ctcaatgtcc aggcagcgga atctgtcgtt atctgcgata gcacgctgta ccatgtcata
     6001 acgttctgtc ccgggcagaa ccgtaatgcc ctcttttgtc ttcagataag aacagcccgt
     6061 cggtatcatc cagatctggt ctaagtgtgc cgactccagc gcctgttcag ccagcatcag
     6121 atgtcctatg tggattgggt taaaggtgcc acccataatg cctattctgc tcatgcttcc
     6181 tcatcccttt gcacgcagtt atcattcttg attggttctc gtcggctatg ctctgacctt
     6241 atctgggcag ttcgatcttc ggcttgtcct tgtcgggctt gtacaataca atcttcttgc
     6301 cgatcacctg tacgacctgg gaatgggtac gctccgccac catctccgca atagctctgg
     6361 ggtcgtcaaa acagttcttc agaacaccga tcttgatcag ttcattatta ttaaaggatt
     6421 cacaaaaagc tgcggtgagt tccggagtca ggctggattt acccacctga aaaatgggat
     6481 tcagattgct ggccagactc attaaataag ctctctgttt gcttgtcata atctatcttt
     6541 tcctttcaca aacttactta tagtaatcaa aggacagacc gtacatgcgt accgtatcgc
     6601 cctcctggat acccagtgct tccagttcct ctaaaatacc ggtatccttc aggaaattct
     6661 ggaagaaggt aaatcccttc tcactgtcca gattggtgta tcccagcatc ttctcgatct
     6721 tgggtccttc tactacgtac tcgtcctctt cttcatcata ggttacggtg taagcatcat
     6781 ccacagatcc tgtcatactc tccgggaagt actcctgcgc aaagacagtg gtctcttctc
     6841 cgatgttagc aagcatatcg ttcacttcgt ataacagttc ctttacgccc tgaccggtta
     6901 ctgcagacat agggaacacc ttgatgccct tcggttcaaa ctctgcccgg attgcagcta
     6961 ccggatcgga tccatcctcc gtatagatag cgtcaatctt attcgccgcg atcacctgag
     7021 gtctggctgc aatctccgga ttataggctt ccagttcttt attgatggca tagatatcct
     7081 gaatgggatc tcttccctcc gtaccggccg catccacgat atggatgatc accttggtac
     7141 gttcgatgtg gcgcaggaat tcgtgtccca gaccgattcc ctcggaagct ccctcgatca
     7201 gtcccggaat atccgcaatg acaaaaccac cggttccttc cagatccact acacccagat
     7261 tcggattcag ggtcgtgaaa tgatagttgg caatcttggg tctggcattg gtcactctgg
     7321 acaggaacgt ggatttacca acattgggaa atcctaccag tccgacatca gcgatcacct
     7381 tcagttccag cagaagttcc agttcctgtg cagcctgtcc cggctgtgca tacttgggtg
     7441 cctgcatggt actggtagca taatgctggt tgccgttacc gcccttgccg ccttttaaga
     7501 gaacaacccg tttgttgtcc cccgacatat ccgtaattac ctgcccgctc tgtgcttctt
     7561 tgatcacggt tcccgcaggg actttgataa tgatgtcttc tccgtccttg ccacggcagt
     7621 ttttcttgcc gccctcttcc ccgtctccgg ccttgtactt acggatgtgc cggaaatcga
     7681 tcagggtatt cagtccctca tctacaacaa agatcacgtc accgccgtga ccaccatcgc
     7741 caccgtcagg gccgccactg ggaacatatt tttcgcggcg aaaggacaca tgaccatctc
     7801 cgcctttacc gcttcttacg taaattttag ctctgtcagc aaacattttg ttctcctttc
     7861 cgacataaaa ctctttacgg actaaaaggg cttcaaacaa tcagtatgtt tgaagccctg
     7921 gttaaatcaa cttatttctc tacaggatat acggaagcct gcttcttgtc tctgcccttt
     7981 ctctcaaaac ggagaacgcc atcaactaaa gcaaacagag tatcatctcc accgatacca
     8041 acattgacac cgggatggat cttagtaccg cgctgtctgt aaagaatatt tccagctttt
     8101 acaaactggc catcagctct cttcgcacct aatctcttgg attcggaatc tctaccgttc
     8161 ttggtagaac caactccctt tttatgtgca aaaaattgaa ggttaaagtt aaacatggtc
     8221 tacacctcct cgatttttac ttgcaaatac tgttccccgt actgtctgct gagcttctgc
     8281 agtgaaaaca ccataaagtc catcagaaat acggtcttgt cctgcggaat ggaattctcg
     8341 aaatcacatt tgataaatcc cgtctcctca ttctgcgtca gctgcatttt ttcgccgatc
     8401 atctcgtcca acgccatgat cgtactctgt acggtcacgg aaatcgctgc acagaggata
     8461 tccggttcct taccgaacaa tctcttcttc gtatattccg catgccccat gcagtagaat
     8521 ccacgataga tcccttcatg gttcttacga atgataatag ttgtcataac gattacgcgt
     8581 taatcttttc gatcttaacc tgagtgtatg cttgtctatg accattcttc ttgtggtaac
     8641 cggtttttct cttgtacttg tatacaataa ccttcttacc tctaccctgc tccataacgg
     8701 ttgcggtaac agttgcgtta gcaacgtcag caccaacctt gagggtattg tcacttactg
     8761 cgatcacctg gtcaaaagta acagtattac cagcttctgc atcgatcttc tcaactttga
     8821 tcacatcacc ttcagaaact ttgtactgct ttccacctgt tgcaataatt gcgtacataa
     8881 taaggcacct cctattattt actcgctaac tttggtggta aaggattcta aaatccgaca
     8941 cacttatacc tagttatgcg gcataccgta gtataataac atgaggtgcc ctaatcgtca
     9001 agatataatt ttattttttt gaaaaatatt tcagatatct acttttccag aatatctgca
     9061 ttatttttct tctcgaagtc ttccacatac tgaatgatca gctttacggt tccttcgatg
     9121 ccgaggatgc tgctgttgat gcacagatcg taactatcag ctctgcccca cttcttggaa
     9181 gaataatagt tgtagtaact ctgacgctgc ttatccttct tcgtgatcat ttcctttgcc
     9241 ttggcttccg tcagatcata tctggccatg atacgtttgg tcttcacatc gtcatcgccg
     9301 aagatgaaca gattgatgca gttcttgtaa tctgccagag cataatctgc acaacggcct
     9361 acaataacgc aagggccttc atcggcgatc ttcttgatcg tatcaaactg tgcaagaaat
     9421 actttgtggc tgataggcat atctacaaaa gaggatgcat tgtagccgaa agaataagta
     9481 tccatcacca gattatacag gaaagaattg gtaggtctct catcatggtt ctggatcatt
     9541 tcctcacaga aaccactttc ctgtgccgct ctgataagaa gttccttatc atagcatttg
     9601 attccgaaat actctgcaac cttttccccg atttcacggc ctgcagaacc aaactgacgt
     9661 ccaatggtaa taatcgtatt catacacaca cccactttct gctgtacttg ataactagca
     9721 ttttttgtat tttcattata cgtgatattt aacaaaaaat caaccaatta tcagtttttt
     9781 cagtcgttta ttactaattt tcatatgatg catgcatttt ttctgctatt ttcatgcctt
     9841 cttccgtctg tgccaggcct cctaacgccg tttccctcag ggaagcatcc attttatccc
     9901 cgacttcttt catggcatcc ataacctgat ccggtggaat ccggcttacg atccccgcca
     9961 ttgccatatc cgcacaggcc atggcattca cagtaccgat cacatttcgt ttcacgcatg
    10021 gaacctccac cagtcccgct accgggtcgc ataccagtcc cagcagattc ttcatagcca
    10081 tggcagccgc tgtagcaatc tgttcgcagt tgcctccctg cagatatgtc agagctccgg
    10141 ccgccatgga actggcggac ccgatctccg cctggcatcc tcccgcagcc ccgctgatgg
    10201 aagaacgggt ggctatgatc tgtccaatcc ccgccgctac atacatggct ttcagaattt
    10261 tgtcggtatc cctgcaccgt ttttgaaaat agggaaccag caccgcaggc aataccccac
    10321 aggctccagc ggtaggtgct gccacgatcc gtttcataca ggcattggat tctcccatct
    10381 gtaacgctcc tgcaatacat tcgtctacat attccccgca tagtgactcc ttaccggtat
    10441 tgtgtgtgta ttcctccatc tgttggccat caccgccgac aagtccgctg gaggacttaa
    10501 tatctccttc ataagtagcc gctgccagca gcatggcatc ccaggtagtc cgcatatgct
    10561 ccatactgtc ctgctttgac acattccgct ccgccatatc atcttccaga atcacctgcc
    10621 agaattcttt tccttccgta tgacatttct gtaatatctg ttcaaatgac tgataggaca
    10681 tagttactcc tccacattga tataggtcac cttaatgatg ccgtccagct gttccagcca
    10741 ggccaccgat tcctccggca cttcctgatc tgtctctatg accataacgg cgtagccgcc
    10801 ccgcttgtcc ctgtagagtt gcagagttgc aatattcacc tgcttttcag ccagcatgga
    10861 ggtcacctgt gccacatgtc ccggctgatc cacattgtgt acaatcaaag tcggcttttc
    10921 cgcagagaaa tttacttcga ttccatccag ttttgcgatc aggatccggc cgccgccaat
    10981 ggaagccgcc tgcacttcta ttttccgccc atgttctccg gtcagccgca gaaccgccgt
    11041 attgggatgg gcatctttca gggtaatggt ggaaaaagag aattccattc catccctttt
    11101 cgccaggaaa aagctgtccg gaatccggat atcatccggc cgcattccca aaaggcctgc
    11161 gatcagcgcc ttatccgtgc catgtccgat gcccgtagta gcaaaggaac catgcagaag
    11221 gatttccgcc ttcaccacat ggtcctgaag taattttcct gaaatataac cgatccggac
    11281 cgctcccgca gtatgagaac tgctggggcc gaccattacg ggtcctaaaa tatcaaacag
    11341 attcatagtc gcctcatttc tgtacgaaac ataagctata agttttattt ctccaacact
    11401 gtcagaagat gttcaaaata ttccggcagc ggagcatcga tctccagata ttctccggta
    11461 gtgggatgaa taaatcccag tgtcttggca tgaagtgtct gcccctgtaa tttgaaagga
    11521 cttttccggt tggagtagac ttcatccccc agaagtggat gcccgatgct tgccatatgc
    11581 acacggatct ggtgtgtcct tccggtctcc aacacacact ctatataagt atatccctga
    11641 aatctctgta atacccggta atgggtcacg gcacgttttc cgttctggtg gttcaccgcc
    11701 atttttttac ggtcattcgg atgcctgccg atgggctggt cgatggtgcc ttcctcctga
    11761 ttcagctctc cgaaacagat ggcacggtat cttctgttga tggaatgttc cttcaactgt
    11821 tctgcaatgc accggtgggc tttgtcattt ttgcaggcaa tgacagatcc agtggtatcc
    11881 ttgtcgatac gatgtacaat accggggcgc agtactccgt tgatcccgga caaatccctg
    11941 ccacagtgat acatcagtgc attaaccaga gtgccgctgt aatgtcccgc cgccggatgc
    12001 accaccatcc ccttcggctt attcaccacg atcagatccg catcctcata caggatatcc
    12061 agagggatgt tctccggttc aatatccggt tccacagatt ccggcacgga gaagactact
    12121 tcatcatcac atttgacacg acaacttccc tttaccggcg taccgtttac cagcacattt
    12181 ccgtctttga tcagcttttg gataaaagaa cgggacagat tctccacaag cagactcata
    12241 catttgtcaa gccgttcgtc ttccatctca tctgctatct gaaatcggag ttcttccatt
    12301 atttcatctc ccgataacga ttctgcttaa agttgaggaa ttccagatcc ttttccttgt
    12361 atacgaacaa aaacagaata aacaggccaa tggtagccac taccacataa atatccgcca
    12421 cattaaagat cggataattg atcagtacga aggagataaa atccactaca taatccagtc
    12481 ggaatctgtc gatcatgttg ccgatacctc cggcaatgat cactgccagc aatacatgca
    12541 cgattcggaa tttcttctgt tccggaagct tgaaaaggaa aaacaacagc accgcaagaa
    12601 atacgattcc cacaaacaga ataaatatct tctggttctg cagcataccg aatgcggatc
    12661 ctctgttctc cagatattgc aattccagaa ctccgtcaat gatcacatag gccggctggt
    12721 tcttcagatg taccaccgcc agatgcttgg taaactgatc cagtaccaga agtacggctg
    12781 caatcagggc atctatgatc aacataacaa ctcgactact tttcttttcc ttattcatga
    12841 gcatcctctt cactaaattt gatttcttcg atggcagcca gaatttcctc atccgtaacg
    12901 gttctgtcga taactgcctt tccgatcttc tttaacagaa taaatttgat cgtaccggct
    12961 tccatcttct tatcggattt tgtaagtttc aagatctcct gcggatcgat atcgtccacg
    13021 gagattggca gataaaacgg tacaaacata tcacggatct cataaaattc ttccatgctc
    13081 agcatttctt ttttccagga aatatatgct gcagctaccg tacccagtgc cacacactct
    13141 ccatgataca attcaaaatt cttgtatttc tcgatagcat gtccgatcgt gtgtccaaga
    13201 tttaagagag cacggtctcc ctgctcggta ggatcctttt ccaccaccat tttcttgatg
    13261 gagcaggtac ggatcaccat ttcttccagg gtattcagat cccgctcgca gatctcatac
    13321 atgttctcga tcagccactc atagaagggc gcatccttga tcagtgcgga tttcattact
    13381 tccgcaaatc cggaataaaa ctgtctctct tccagcgttt ttaatacgga caggttcata
    13441 tataccagtc tgggcatctt aaatgcgcct accatgttct tatagctgtc aaaatccaca
    13501 cctgtcttac cgccaataga gctgtctgcc tgtgccagca atgtagttgg aatctgtaca
    13561 aaatcaatgc ctcttaaata agtagccgcc acaaatccgg tcagatcacc gcagacgccg
    13621 ccgcccagtg ccagcagcat atcttttctg tcaaaaccct cttcaatcag gaaacggtac
    13681 acgtccctca cggtatccag attcttgtgt tcttctcctg cggggaacac atatttcaca
    13741 gccttacggc attttccctc cagaaactta agaatctgtg acgcatacag tccgtctacg
    13801 gtggaatccg tcacaataca taatctcttg tctgctgcgc caagctctgc cagttcttcc
    13861 cacaggccgt caaaagactg ctggaataca atatcgtagc aaggcttttt gttataaagc
    13921 accggtaatc tctgtgccat gttgttcacc cttcagttcc ttataaactg ttctttcttg
    13981 tataatttat atctgttttt aaaaaacttc ctgcagttgc ccacaggaag ttatcttcat
    14041 atgacatttt aatggcattt gacattttat gacattccat tgttgatcgg catttcaaaa
    14101 gatccggcgc cgccgaaaat atcctgaaaa tcaccggaga tatctacgct ggccggcgta
    14161 ataatgaaaa tataatggga tattttctgt aaattacccg atattgcgaa acaggatccg
    14221 gaagtaaagt caatgatacg ctgggcgata tccacgtcca ggccttccag attaagtacc
    14281 actgtccggt tggccaacag tgtctctgtg atctctcttg catcctctac ggaagtaggc
    14341 ttgatcacac agacctccat gccggttcca ggcattttct tggtcacggt gcggtggatc
    14401 ggtgttacct taggctgtga ggaacgctta ctcttctctt ccggttcgtc atcctcctgt
    14461 gcctgctgtc tgctcttggc cgggctgaat ttctttaccg gttctacttc ttcctcatca
    14521 tccagataat cgtcatcata gaaaccttca tcctcttcat cgttcagttt catataattc
    14581 aggaacttat ccattacacc cattgttgtg tgtctccttt attattcaat ctctttctag
    14641 atcgtcttac tataatccct ttctccgaaa ataccggttc cgactctgac gatcgtggct
    14701 ccttcctgcg tggccaccat ataatcgccg gtcattccca tggaaagaat attcatagaa
    14761 atattatcaa tgtttttatt tccgatgtca acagctaatt gccgaagttt ctgaaaatag
    14821 cccctgtttt cttccggatc ctccacaaaa ggggcgatcg tcatcaatcc tctgatatgt
    14881 attcccggaa gcgttgcaat ctcacggatc agtgttgcag tttcttctac cgtagttccg
    14941 aacttgcttt cctcctgtgc cacatttacc tcgatcagga tattgacttc cacctgcttc
    15001 ttcacggctt ctctgctgat ctcttccgcc agccggaggc tgtccacgct gtggatcagg
    15061 taaaccttat ctacaatata ttttacctta ttgcgctgca gatggccgat catatgccat
    15121 ctgacatccg aaggcatctg agggatcttg tccaccagtt cctgaacctt gttctccccg
    15181 aaatctctgg ctcccgcacg gtagacatcc atcagcatat ccaccggttt ggtcttgctg
    15241 actgcaagca gcgtcacttc ctgctccgat ctgcccgcct gctcacaggc ttttgtgacg
    15301 ttctctttta ccgttttgta attttgtgcc agttccatgc tctgtatggt ctcttccata
    15361 gtatccgcca tcctttctag ctttccggtg tctgactttc ctgtgttgct tcctgtgtgg
    15421 tctcctgcgt cggttcctct ctggtctccg ctggaagtgg ctctgtgctc tccggtatat
    15481 tgtcgcttac tcccggttct acactgtctg taggaatatc cgtcctgccg gtatctgttt
    15541 tcttgatatt ggtgatgatc tgatcatccg ataccgaaga accgtccagt acaatatagt
    15601 catatacatt caggccatag gtcgtattgg cttttacgat ggaatactcg tcattctgat
    15661 acagaatatt gatctgcttg aagtcagcat atcccttatt cacattatac acaccgatca
    15721 gtgttgcacg cttgcttacc gtataggtct cctggctgtc tgtcttgtac aggacatctc
    15781 cggccttcag caccgaagta tccatgtagt acacgccttc attttcatcg tagctgtacg
    15841 caccggcctc cacaaattca ctggaaatgt ccccgttatc ccggtagcac tgtctgatca
    15901 caccctgggt tcccgtattt tcattggtcg tgacaaaatc ttcatcgatc aggaaaaatt
    15961 ccttctccac aatggaagaa tttggaatct tcagtccggt ctcatcattc aatatcagct
    16021 cgatatccag aaatctgtcg gatacaaacg taaccatgga atttgtaaag gtaagtgaca
    16081 gaaatgtatt gccatcactg ccggtaaaca gctttgcctg cccccaggac tcatactgat
    16141 tcttcagaaa acgtactttg acataatcct cctgttggat ctctgcgcct cgctctgcat
    16201 cgatgggaat gaccaccgac cagttttcct ccgtggaaag cttgtacacc ggatctccca
    16261 cagccatcag ttcattaccg agcatgtggt tcttctggta atttttgtta tctaatgctt
    16321 cctgggtaac accttcgacg ttcagagttt ctt
//
LOCUS       DAWDHD010000043        16327 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_240_length_16327_cov_11.700467, whole genome shotgun sequence.
ACCESSION   DAWDHD010000043 DAWDHD010000000
VERSION     DAWDHD010000043.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 16327)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 16327)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16327
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_240_length_16327_cov_11.700467"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3964 a         4482 c         3408 g         4473 t
ORIGIN      
        1 tattcttcgc tatcctgtta actgccaagg gattgtacaa tgccatcgca ggcgtaattc
       61 ccgcacagaa ggcggatgtg aagaagctgg cgcaggcagg tctgttagca gctctgtgct
      121 atatcggatt tgctttcttc aagatcgaca ttcccgtggg tcccgagaaa acagcgttcc
      181 atctgggcaa tgtgttctgt gtactggcag ctttattact tggcggatac tggggcggac
      241 ttgccggtgc catcggcatg accatcgccg atctgaccac cgcttatgta accagtgccc
      301 ccaagacctt cttattaaaa ctgtgcatcg gtctgatcgt aggactggtt gctcacaaga
      361 tcttccatct gagcaaagag cattccgcaa aatatgtgac cggtgtaacg atccttgctt
      421 ctgcctgcgg tatggcattt aacgtggtcg cggatccact ggttggttac ttctataaaa
      481 tgtatctgct gggtgtgcct caggatattt ccaaggctct tgccaagatc agcaccgtaa
      541 ctaccggtgt caacgccatc gttgctgtga tctgtgcttc cgtattctat ctggcacttc
      601 gtccggcact gaagaaagca ggattattcc atgacatcta atttcgatac ttctgataga
      661 aatgcacaaa aaggcgggga tactgttccc cgccttgcta tgatcaacga tattgccggc
      721 ttcgggcgct gctccaccac cgtgtccctg cccgtgatca gcgtaatgaa ggtacaggta
      781 tgccctgtgc ctacttctgt tttgtcgaac catttgggat ttcctctctg ccattttgat
      841 gactacacct ctcatatgcg agattatata aaagtatgga acgaactggg acttaccttc
      901 gatgggttat actgtggatt cttaggaaat gaagagcaga tcgacattgt acgggaattc
      961 gtggaaatgt tccggccgcc cctgtttttg ctggatccgg tcatgggaga ccacggcaga
     1021 gcctactcct ccattacaga gacccatgtt cagaagatga aggagctgct gcctctggcg
     1081 gatattatca cgccgaatat cacggagtcc tgcctgctga ccgggactcc ctggaaggat
     1141 ggggaatgga cgctgcagga gctgtccgga ttatgtgaac ggctggctga catttgtcaa
     1201 acagcttcca ttacatccga cgaagcctcc acgggcacta ctgccagtgg ttctgacggg
     1261 gggtccgttg gcgctgtctc caatggctcc gccggtgcat ccatcgtcat caccgggatc
     1321 cgtcagggcg attctctggt aaatttcctg tgggatgacg gtgtctatac cactgtttcc
     1381 acccccattg ccggagcatc ccgtcccggc accggagaca tcttcgcctc cattctcgcc
     1441 gccgatgccg tccgcgggga gaccttacta tcctccgtgc aaaaggcagc gaacttcgta
     1501 ggtctgtgca tcaccggaag tgaaaaggcg ggaacgcccg tgcaggaggg cgtggtgttt
     1561 gagaagtatc tggcggcgct gttgtaaaaa aggcgacggc tttttctttt accgtcgccc
     1621 gacagagcgg attatttagt cattccaatt gggttcacca acattataac tctgatctgc
     1681 aacaggttcg ttagggttat cgtagcagct ttcaatagtc aggtgaaact ccggtaagct
     1741 atcacaagtg ttcgacttat tttccagttt acttgatgca tcgtttccgg ttacattctc
     1801 tgttgttccg cgccccgtgt tagttctata accgagaagc gttcccatgt tatcccgata
     1861 gtaggtataa atgttctgaa ctcgttccca aacctgctcg tcaaaataaa cgtcgctcct
     1921 ttggctaacc atgctttcca catactgacg aagatcggaa tagaacaaat cgctgacatc
     1981 atagcagaca tactgttcgt cctcatagat cttgtactgc tccatctgtt caaaggtcaa
     2041 aagttgaccg cggataaagg catagtcctg cgtggtatga atttcatttt tttcgtatat
     2101 gaaaacataa atcaagggaa tatccacacc gccgtcgatt tcacctttgt gctgcgggat
     2161 gtaggcgctt aaaaccatcg cacattcttc cgcggaggcg ccaatgattt tgtcataccc
     2221 atccgttgat cggcgatgca gaccagttag ctgcaattgc atatccgcag gcacagcagg
     2281 gtcaaaaagg actctttcgg cgctgtcaat caaagttaac tcatttggtg acacggaggg
     2341 cacgatgttt atatccattt gttccaacac cctctggcaa tgttccagat attcgccagc
     2401 gagccgattt ctttcattaa tatccaaagt gtagctttca aagtatggtc tgaattcttc
     2461 cataatttct gatatcagtt ccggatcagc gtcaaccgga gtaatcggcg ccacgtcaac
     2521 tttttcggtt gggacagatt ctgcaaattc aaccgtacac atccaatcct gtccaaataa
     2581 gaagttgtta tttgtcaaca ccaagtagta ccaatcatca tccgaaatcg gtgtctccaa
     2641 cagtgatata tcataggcat ctgaaagatc aattaccatc gtaccgcttg agttagccgg
     2701 tatcccggtg ttgctgaaag acacctttcc cgaaagcggt ttaacttcct cgctggtaga
     2761 ccagcgcatt agtttaagtt tcgattgaaa attaagctct ttgtcagacc tattttctac
     2821 ttgaacagaa attacaccat ttccctcata tgtggcagac agagaaagtt catctccgga
     2881 tagggaggaa aacaaactgt ttgcaaaaac agtcatagaa aagaaacaaa aaactgctgc
     2941 aactactccc catttcaccc ataccgtctt tttcttcacc cgtatgttgg gagctgcttc
     3001 ctgaatatac tttgtgctga tgttaccaac cgcctcggaa atatcttcac gtttcatact
     3061 gtaaatccct cccccttcaa ataatcccgc agtttgtccc taatacggga aagtcgcact
     3121 gacacggtat tactcttcag accaaactgt tttgcaagtt ccgaaataga ataggcatac
     3181 caatatcgac gaacaaagag tattcgactc tctgcatcca gtgtatcaag aaaatagtca
     3241 atctgccggg taagctcctt tgcggaaatc tcctcctcta cactttcaga ggaggataca
     3301 cattccccca gttcgtctaa agctgcatcg taaaagctgt tgcgcttgac agatgtattc
     3361 gcatgatact tttttatgga gaggttacga acaattcgac agatataggt aagcaacggg
     3421 tttggatttt gaggcggtat tgtattccat gctcctaaat aagcgtcgtt aacacattcc
     3481 tctgcatcga ggatattgtt caaaatattt tttgcgacct tgttacagac atttccgtac
     3541 ttcgccgcca actccatgat tgcccgttca gagcgcatat agaacaaatc aataatttca
     3601 ctatcgttca aatcatcact tcctttctgc ctcacttata tactacgaat taagagaaag
     3661 atattacaag aaaaacaaat attttcaaat ttaacagaca gtgtctggga tattgagtat
     3721 ccattcctga tgatcgaaaa aagccagatt tctacttttc tctatgaaag ctaattacct
     3781 tttgtgcctt ttcatttata acaattttag agtcttccgt attcactttt acctcccatg
     3841 gtatcataag ttcaatttta gctttgaata tactccaagc ggtattattt ttatcaaatc
     3901 atagatagaa ctttcaaaaa taccgcctga tggatagtaa ctccattcag gcggtattat
     3961 aacaataatt tttcaaatga aagaagcttt tttactgctg cgatataaaa gtaacgctgt
     4021 ccggcagtat tttattcaaa ctccaccaca acgctgccca tagcacattc cagctccata
     4081 tttttagaag cgttgttgtt gatctgtttc tcctgtgcca ggccgttgta actgtcatca
     4141 ccgactttca gttcacccat accgcagctt aaatcgtagt tgaaatccgt ctgcgttcct
     4201 gccagtgtca gtttcagttc tcccatggag cactctccgt ccaatcgttc ggtcacatct
     4261 ccgttcagac ggacggatcc cattcctact gacacctctg ccacgcggct gctcagttct
     4321 tctactgtca tctgaccggc accacagtct aactctacct ggtcagcctc cagtttctca
     4381 aacgtcatct tgccggcacc cacattggct tccagcgccc ccgtctgcaa ttcctctaca
     4441 ctgaggcttc cggcgcccag atccaaagag gtattttcaa aataatatcc ctcaggcaca
     4501 tacaggcaga tggaaccgcc cagattacct tcactgttgc tgcttacttt tgaggttccg
     4561 cggatctcca tcgtaccgcc ttccacatat ccctggaact ttcgcattcc atcggcttct
     4621 acccggaagc agttatcctc tgacacctga atcttcatga cacagccacc ggcctgcacc
     4681 tgtagatccg tgatgccctg actgtcatcc cccagaacat acagctctat ctttcccgac
     4741 ctaacttcat aatctgagtc ataatccaca ttgtcctcca gatcatagtg cacatcgccc
     4801 atattttccc ggatctgctc cgtcacaccg ttgctccagt catccacctc ttctccggag
     4861 attcttccca gggtaaccgc tgacaggatc tcccccggcg aaaaatgcgt ggagccattc
     4921 cccaggcttc cgctcactcc caggatcagt cccaataata taaatatcaa cgctgtgatg
     4981 gcacagccct tcataaattt tctcatgctt cttttttacc tcttttctgg aataatcctc
     5041 cgatccaaca acagatcgcc ggtgtagcga ttcccgccat ggccacggtc agcatcagga
     5101 acagaagtcc gacacctccg cagatcagtc cggcaccgat caggcacatt cccacgaagg
     5161 gatgcgtaaa catactgatg atccctactg ccaccagtgc gatcagaatc agtatcagtg
     5221 ctgctgccgt tgctccgaaa ccgatgatca gtccgaacca tgtggcgatc agccctgctg
     5281 ccacgccgat accggcggaa gctgctccca ggatcaccgg ggatgccaaa atacataaaa
     5341 ttacgatcag cacgatcata ccggtggaca ttccttcttt attctgtgga gataccacct
     5401 gtgcctgtgc agtttgtgac gcatcgctct gccagctgcg ctgttctgtg ccgccgtctt
     5461 cctcattgga atatgccggg tagagaatca ttgctttggt ttcggcattg atcttctggc
     5521 agctattctc tccataacca ttcccgaaaa tgtctttttt gatattctcc gctactttca
     5581 tcggattccc cagtgcctca ataacattct gttcattctc cggtcccgca tcgttaaaat
     5641 attcattata atattgtagc gcttcttccc gctctgtagc ggaaatattc tgcaacagac
     5701 tttccagctg cctcataaac tccgttctat tcattctact atgttctccc atctgcgcct
     5761 gtttacgcct ctgacctgtc acttagtttt ccttcaaaca atcccgtgat cttatcgctg
     5821 tagctgcgcc actcactttc atataaatga agctgcgccc aacctctgct ggtgactttg
     5881 taataacgcc tcgttctgcc actgcattcc cggtcataga cttccagaca ttcgtccttc
     5941 tgtaatcgcc gaagcacggg atacagggtg gattccgaca attcgatcac ctgccggacc
     6001 tcctgcgtga tcttgtagcc gtaggttcct tccgattccc gggccaccac cgccagtacg
     6061 atagcatcca gcagagctgc tcctgtatta aaaagcatcc tatcgctcct ctctgaaatt
     6121 tgcggctatc cgcctttcct tacaatatta tatgccgtat agtattgctc tgtggctata
     6181 ctatacggca tataatatca tgcgtcaata tagagaaacc gtaagaaatt ctaaagactt
     6241 tccaaagatt ccgtcgactc ctccagaaac tcctgacgga tactgttttt atggatcaca
     6301 atggcagaga tcagaagtgc tccccaaaat acctgccgtg gtattctctg tatcatcaac
     6361 agaaccgctg ctcccacggc aaaaaagata taagtgatac gggttcttct ggtgtgcggt
     6421 ctgatcagaa gggcagaccc cgacaaatac aatatgatca gcaacagcag cagttccggc
     6481 tgtaccggtg ccagtccgat caacacgccg aaggataccg caatggcttt gcccccattc
     6541 ccatgataaa acagggaata ggcatgcccc agtaccggtg ccgccatgat aagcgggaac
     6601 cagctgtctg tcacaagccc catgccaact gccatgtata ccggcagtgc tccctttaac
     6661 agatctccca gcaggcacag gatcccgaca ggcactcctg cgtatttcat ggcattggca
     6721 gtccccggat tatgatcgtt gctgacctca gtgacatcaa ttcctttgat ccacttcggt
     6781 aataccctgc cgaacaatat acttccgcat aacaatccga agactgtcat tcccagttcc
     6841 cgtatgatca tctgtctctc ctgtctgccg ttttcctctg tgcctaagct tccttctgtg
     6901 ctgcttccgc cgcttccttt tgtccgcaga taaattcaca gactgcgtct gcagcataag
     6961 gctttccgta tttttcctga cacttccgca tttcggcctg tgcctccgga tcccgcagaa
     7021 gctttagtcc tttttgtatc acggcatctt ccgtatctcc tgatacagac atcccatgtt
     7081 gtgtgaaaaa agcaacatta cgatcctcac atccggggat cggcttagtg tgtaccaatg
     7141 cagctttcgc agccaccgct tccgtagagg tcagtcctcc cggtttggtg aacaaaatat
     7201 ccgccgctgc catatagaga tgcgcttccg tggtaaaatc cagcacatgc acccgctctg
     7261 tatcggcata tctatcacgg agtgtcttct tcagctcttc attggttccc ccaaggatat
     7321 acagatgggt atcctcgccg gtctgctcca ccagctttgc cgtcatgttt tccacacggc
     7381 cgtaccccat actgcccgtc atcataagga tacaggattt atctgccgga atccccagct
     7441 gcactcgcgc ttctcttttt tccggaagct tcaggaacct ctccgatacc ggaattcccg
     7501 tgggaaccag tttttccgca gggatgccgc gtttgatgaa ctccgttgcc agttcctcat
     7561 gcgggataaa atagtaatcc acctttgtct cctcggtaaa aggaatacag gtataatccg
     7621 tggcaataaa ataagtctgt acctccagct tatgctgtcg ttgcagccag gtcattgcct
     7681 cagccgggaa cagatgcggc attaccactg tatcatatcc gttttctgtg atatagctgc
     7741 acagcttatc cgcatacaaa gcgttggcaa aatacaccgg tgactttaat ctggcagacg
     7801 aaatgaccct gcctacccgg tacgctcctg cgaacacctt tttcgcccgg accgtactcc
     7861 agatgtagat ccggcgtccg taagcgctgg ccttgtccga agcaaaatta agtacatccg
     7921 ccaactcaca gggaatgccc cgtttttcaa actgttcttt caccgctttt gcagcagaat
     7981 tatgtccctc accggttccg gtggataaga tcagtacctt cataaataaa ttctccgtta
     8041 gttttccatc agccagaaat acgcactgta cgcaggcaga agactgccgc cgccaaagtc
     8101 atgtttctct ccggtgatca gatccaccgc cgttccgcag ccggcatcaa aatgtgccat
     8161 gaagtcttct ccgtctgcat tgatagccac cagtactctc tcgtgctccg actttctctc
     8221 gaaaatacac tgcttattgg tcaacagtac cgaacgaaag gctccgtaat tcagtgcctc
     8281 ggaatgcttc ttggcctctg ccaatctgct gatccagtca cacaggccgt tccactccgg
     8341 cttgtcaaag cagggacgca gtgcagggtc tccatcttct tttctggctt tgaagcccca
     8401 ttcgcttcca tagtagacac aggggattcc cggcattccg aatgccatgg catagatcag
     8461 gggcagatgc ttctcattac tcaaaatgct ggccactcgg gtcacatcgt ggttatccac
     8521 aaaagacagc aggtgttttc ccttatacag tgtccagttc tccggtccga actggcgcag
     8581 cagggaatgc acgatctcga acatgttcat actgttgaag ctgctgtaaa gtcccttata
     8641 gcactcgtag ttcgtcgcag aatgtaacat ctgatcattc atcaggcggt tataatctcc
     8701 gtgaagcatc tcccctaata agaagaaatc ctgcttgtag gcaccggtca cttcccgcag
     8761 tcttctgaca aagttctcat ccaggctgta cgccacatcc agacgcagac catcgatacc
     8821 gaattcatcg atccagccct tcacgctgtc taacaggtaa ttcactacct ccgggttctg
     8881 caggttcagc ttcaccagat caaagcagcc ttcccagccc tcataccaga gtccgtcgtt
     8941 gtaattggaa ttgccaccga agtcgatccg gtagaaccag ttcacatacg gtgaattctc
     9001 ccggttgcgg atcacatcct ggaactgggc aaagcctctg cccacatgat tgaacacacc
     9061 gtctaacaca acccggattc ctgccgcatg caatgcatca catacttcct taaaatctcc
     9121 attggttccc agtcttacat caattttgcg gtaatccctg gtattgtaac catgagtatc
     9181 cgattcgaat accggggaga aatataccgc gttcactccc agcttctgca tatgggggat
     9241 ccagtcctcc acacgtttga tctcatgtct ctgtactccg tcattttcaa aaggaactcc
     9301 gcagaatccc atgggataga tctgataaaa aacactttca taagcccaca tattagtcac
     9361 catacctttc attgactgat ccagtaccgt tttgtttttt tatccacaac ggttcttttg
     9421 ctattataca ccatttttga accgtctgtc gttttttctg tgtaaaacca caaataatcc
     9481 acaaaatcca ccgacttatc cacatataca catgtgtcat atgtggttat gtggattgtc
     9541 atatcggata tcaactcgtg tatcacattg aaaatcatcc cttttgccac aaccaggcat
     9601 cccagaagcg ctccaccttt ttggctaccg tatccacggt cacaggctgc acctgttccc
     9661 actctctggg gaataatctc cggtaggaat actgctgaaa ccgatcatcc agcagaacaa
     9721 tgatacccac atcctccgcc gtccggatca cgcggcctgc cgcctgcaat actttattca
     9781 ttcccggata gcggtaggaa taatcgaagc cgttctctcc gtcctcatca aaatagccct
     9841 tgaggatctc ccgctcacag cctacctggg gcagtccggt tcctactaca atgactccga
     9901 tcagactgtc cctcttcaga tcgatgccct ctccgaagat tccccccagc acgcagaagc
     9961 cgatcagcgt ctgctcctct tcctcctcga tctccatacc gatgagactt tgcaggtcac
    10021 attcctcatt gccccggaag cgattcagga agtattcacg ctcttcctca ttcatggatt
    10081 cctgttggac gagacattct tcttcctctg tcataaaata ctgttcatag atttcgtaaa
    10141 tatgattcat aaaggaatag gacgggaaaa atatcatgta attgccgtgc cggttcttca
    10201 ccacttcgtg aatgtaacgg gcaatattgt agtattcttc ctgggatctt ctgctgaact
    10261 tgctagtcac atccccggcg atcagtatgg tacgcttctc gggatcaaac accgagtggg
    10321 catacacctc gtaatcctcc ttctcaccgc ccagcaggtt cttgtaatac tggatgggca
    10381 ggaaggtagc ggaaaaaagg atcgtactgc gtcctctgag catgcattcc ttcaggtttt
    10441 ctctgggatt cacgcagaac agcttcagtt caaagctgcc atcctcacac aatctggtat
    10501 atttcacata gttttcatct tgtctctcgt aaatatccag aaaatgactg agcttgaaat
    10561 agaagtccag caggttctcc cgtacttcca ggctgacctt ctcctgctcc tccagataat
    10621 cactgatcac agcgtgcagt ctggtcaagg gctgcaccag catgtcaata tcatccacca
    10681 gccggtaccc gtcacagtcc cgcttcatag acagcagctg tgcgttgcat ttctccagca
    10741 gctggctgat ccgttccgca tagccttccc gaacaaaggt actctttccg tcagactttt
    10801 tccctttgtg tccccttaca tagagggaat cggtaccgtc tgtttcctcc agggtaatct
    10861 cttgtggcag ttgttttgac atacctgtca tttctaatgt catctgcccg gaaatcccgt
    10921 ctttatcacg ctttttctgg gctgcttctt ccatttccga cgtgattgtc tgtttgatct
    10981 cccgcttgag ttccaacaga tcttccttcc gtagcggtgc actgtacatc tcccggcccc
    11041 gctctaagag gttatgtgcc tcatcgatca gaaatacata attaccctga ctgccgtccc
    11101 cgaaaaatct cttcagatac acatggggat caaagaggta attataatct ccgataattc
    11161 catctgcgaa cagacttaaa tccagtccga attcaaaagg gcatacctga tgtttcaggg
    11221 catattcttc tattttgctt ctggtaaagc tctcttcgtg ggtcagaaga tcatagatag
    11281 cctcattgac acggtcaaaa tgcccctttg cataagggca ctgctccgga ttgcactccg
    11341 tatgaccctg aaaacagatc ttctccttgg ctgtcaggat aatgctctta aagcgcagtc
    11401 ccttctgcct gagcaaagaa aaggtgtcct ccgccaccgt ccgggtgatg gtcttggcgg
    11461 tgagatagaa cagcttgtct cccatatcct ttcccatggc ctttaccgtg ggaaagatcg
    11521 tggatatggt cttaccgact cccgtaggag cttcaataaa taattttctc ttatgataaa
    11581 tggtctggta cacatagctg accagctctt tctgtccatc ccgatagtcg aagggaaact
    11641 gcagctcagc aatggactgc tgccgcaatt ctctccactg ccaggtatag tccgcccatt
    11701 tgcggtaatc actgatcagc tgctggaacc aggcttccag tctgacgaat tcataatctt
    11761 cataaaaata gcggatatct tccgtctcta tattgcaata ggtcagccgc acccggatat
    11821 agggcagctc cttttgcagg ctgtacatat aggcgtagca tttcgcctgg gcaatatgta
    11881 aaggcaaggg tgccttcagc ttcgacagat ctttataggt tcccttgatc tcatcgatgg
    11941 tggtctctcc attctgctcg atgatgccgt cggcccggcc ttccacgatg atgacatagt
    12001 cctccgtggg atgggtgtat tttaatgcca cttctgcctg atactccgca cccatacggc
    12061 gctggatcat acggtggatc cggctgccct cctgcatggc attctccggg gatccctgcc
    12121 tcctgtcgtc gatgtcgcca ctgcgcagga tgaactctac aagattacgt accgatattc
    12181 tgacttctcc tgccatgtga cttcacctcc ctcaaacaag tccctacgat acccagatgc
    12241 ctacggcttg ttccgtgctg cgcactctca caagcctccc cactattcgc atatcgtggg
    12301 ggcggggcta aaggtctttc atccacttat gagcaagctc aagtggattt agacctttgt
    12361 cccctggcat catataataa aactacccag gtaaatagta ctctatctta cctgggtagt
    12421 caaatgacat tctgttgttg agaaatactg acctgtcagt gcattaacat gcaatcgcac
    12481 actgcttcag catctcttcg aatttatcat tgtagccgtc ataggacacg gtcaacggct
    12541 tcgtgaaatc caatccatac actccaagga atgatttggc atcgattacg tatctgttat
    12601 agcatatatc aatatcaaaa tcacactttg aagctacatc aacaaaatgc ttcacgtctt
    12661 caggtgtcaa acggatcgtc tttaacattt cttgttcctt cctttcaata gtaagttcga
    12721 tcgtaagtaa aataatacac cgggtacttt cccctcacat ctgaaatcat tactattata
    12781 gccaaccatc cccaaatgta aagcggtttt tttcttccag aaaacgcaca ttttgggcat
    12841 ttttaccact ctgttatcat tttcacaata tatgttcgcc gtatggaaaa ccttttcggc
    12901 gttttgtata tttgttgcgt taccgcggta aaatataaat cccaatttgg cgaaatctac
    12961 cgaaattcca ccatatattc ttggaacctc agcggaagca tatttcgttg cagatccctg
    13021 ttttcacgtt cttttatggc tattgtatga cagaaggaac ggaaaagttc ccggtagtgc
    13081 tgttccgcag cggacagttt ctccgtctca gactgtactc tctcccaggc atccttccct
    13141 tgcagcgtat accagggctt tcccgccgga tgtattccga acagataccg gcctgcatcg
    13201 aacaccagaa agtcttccat gggaaagcgg tctgcaaaat gcaccatcag aaaggtcaga
    13261 atattattct tcggcgcgat cttcgaatag aggattgtat tctccagttc ttcaaaccgc
    13321 gtaaatcctt tcagatggtg atactcattt ccggcgcctc tggccaggct gaaggtctgg
    13381 tttacatacg tgtctgccat ggcatccagc agatgtcccg gcggacactt ttcggaaagc
    13441 ccctttgcaa tggtacggta taccgcctgg gccttttccg gcttcgggga tgaaagagcc
    13501 agacacaggc tctcataatc ttcctcccca aatctgcgtt tcaatgtccg gatcaccttc
    13561 accgtcttct ccggatccgg tatgacctcc accgactccg agaacagcag gttctcctcg
    13621 atggtggtga ccatagtatc ccggatgata cagtgatctt cataagtatt ataaatagcg
    13681 gtgaagattc cctccagcga atcctcacag tgataaatct tcatatgatc cgtttctcct
    13741 cctgccgtac cccgttcggg aacatcccat catcaaacaa ggtcatctgc cggtaactca
    13801 tgccatcgct gccgaaccgg atccgttcct tcggatccgt cagattccgc acgatatagt
    13861 cctcctccag cttcgtggga tacatcatcc tgccaccaca ggtaataaaa taaacagccc
    13921 gtttcatcac cacgcccagc ttcttcagat cctgaaacgt aagctttgtg ctcctcctgg
    13981 cactaaggat ccgtctggcg cttttcacgc cgatccccgg cacccgaagc agatctgcat
    14041 aaggtgcccg gttgatctcc accgggaaac attccagatg ccgcaccgcc cagtcctcct
    14101 tgggatccag catcacatta aaaaacggtc ttttctcgtc caacagttcc tccgccttaa
    14161 acccataaaa ccgcagcagc cagtctgcct gatacagacg gtgctcccgc agaagcggcg
    14221 gccccccggg aagcgccggc aggctcttgt cctcattgac ctttacaaaa gcggaataga
    14281 acacacgctt taactcaaac ttctgataca gattctctgc tacatttaaa atctgataat
    14341 ccgtctccgg ggttgcccct atgatcatct gggtagactg cccgccactc acgaaaacag
    14401 ggctcttgcg gtacaggatc agttcctcct tattcgcatg aattccattc tggatctgcc
    14461 gcatgggcgt cagaatattt ttccggtgct tgttcggtgc cagtgtccgc agcccctccg
    14521 ccgtgggcag ctccaggttc acgctcatac gatctgccag ataccccatc cgctccacca
    14581 gttcgggatc cgctcccgga atcgctttta catggacata gccgttaaaa tggtattgat
    14641 tccgtagcag ccatatggtc cgatacaaca attccatggt aaaagtagga tcctgtatga
    14701 taccggagct taaaaacagt ccctcgatat aattacgccg gtaaaactcc atagtcagtg
    14761 tacagatctc ctccggggta aaggtggctc tgggaacatc attggacttt cgattcagac
    14821 aatatttaca gtcgaagata cattcattgg tcatcagaat cttcaacaaa gaaatgcatc
    14881 tgccatccga agaaaagctg tgacagatcc cggccttcgt gcagttcccc atgtgcttcc
    14941 cgtcgccctt acgttccact ccactggaag tacaggccac atcatatttg gctgcatcgg
    15001 tcaggatctc cagcttctgc atgatggaca attccccgtt ttgtataaga tattccacat
    15061 tatcaccgcc ttttaccgaa aatttccaga atcagaacgt ttgttcttgt gtatattcaa
    15121 gttagcacat atgttcgatt tttgcaagtg gttatttttt ccgaaaaaag gcttgcaaaa
    15181 tcgtgcattt cgcagttaaa taatatagga atattttgta acacacgccg cccggcggca
    15241 gaatgagagg tcagtatgtc agatattgtc atacctaaaa attataagtc tcagctgaac
    15301 ctgtacgaga cacaggttgc cattaagaca gtgaaggact ttttccaggg attgttagca
    15361 gagcgtctgc acctgctgcg tgtatctgct cccttatttg tggatcctac atccggactg
    15421 aacgataatc tgaacggtgt ggagcgtccc gtgaatttcc atattgcagc acagggagac
    15481 cgtgaagcag agatcgtaca gtctctggcc aaatggaagc gttatgcctt gcagaaatac
    15541 ggttttcagc ccggcgaagg tctctacacc gatatgagtg ccatccgtca ggatgaggag
    15601 accgacaaca tccattccat ctatgtggat cagtgggact gggaaaagat catcacgaag
    15661 gaagagcgga atctggagac tctgaaagaa accgtccgca ccgtatacaa ggttcttcgc
    15721 aagaccgaga agtatatgtc catccattac gactacatcg aagaaatcct tcctcatgac
    15781 atcttctttg taactaccag tgagctggca gagatgttcc ccgactactc cccgaaggag
    15841 cgggaatact acattgccaa ggcaaaaggc gctgtctgca tcatgcagat cggtgagacc
    15901 ttagagaatg gcaagcctca cgatggccgt gcaccggact acgatgactg ggcgctgaat
    15961 gctgatatcg tggtatatta tccggttctg gacattgctc tggagctgtc cagcatgggt
    16021 atccgtgtag acaagaaggc tctgttagac cagttacaga aggccggctg ccctgagcgt
    16081 gcagaacttc cttatcacaa ggcagttatt ggcggcgagc ttccccttac catcggcggt
    16141 ggtatcggtc agtcccgtat ctgtatgttc ttcctgcgca aagctcacat cggtgaagta
    16201 cagtgttctc tgtggtccga tgaagttatg gagactgcaa aagagaacgg cttacagttg
    16261 ttataacatc ataatcgagt aggaggcggt gattaaccgc cgtcctctca caccaccgtg
    16321 cgtaccg
//
LOCUS       DAWDHD010000044        16299 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_241_length_16299_cov_12.661906, whole genome shotgun sequence.
ACCESSION   DAWDHD010000044 DAWDHD010000000
VERSION     DAWDHD010000044.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 16299)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 16299)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16299
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_241_length_16299_cov_12.661906"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    15082..15181
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4280 a         3505 c         4531 g         3883 t
ORIGIN      
        1 atatgcaact tattatacca cgcgaaagga taatgcgtgg gcaatgaagc atccggagga
       61 gatccagcag gaatacctga tcagcaaccg catcaccgcc agaggggaaa cactccgcat
      121 ccgtctgatg gaaggcttcc atacggagca gcttaaggtc aacacgctgg acgatccgaa
      181 gcgttggtgg gaagtgatcg acagaacgac cggtgaagtg gtgccgacgg atgcatggga
      241 atttgatgag gcatccggtg aagtggagat ccgtacgatc ccctatcatg agtatacggt
      301 aagctttctg gcatttttga tctgggatcc ggtacacatg tacaatttca tcacgaatga
      361 ctggaaggat acaccgcatc agctcactta cgatgtgaga cagcccaaga ccaaacagta
      421 tgtgaaagac aagctgagaa aatggtgtga agacaatcct catatcgatg tggtccgctt
      481 cactaccttt ttccatcagt ttacactgac cttcgatgac aagaagcggg aaaaatttgt
      541 agagtggttt ggatacagcg caagtgtgag cccctatatc ctggaacagt ttgaaaaatg
      601 ggcaggttac aaattccgcc cggagtacat tgtggatcag ggatatcaca acagcatgtt
      661 ccgcgttccc agcaaacagt tcctggattt catagagttc cagcagatcg aggtatgtgc
      721 gctggcgaag gagctggtag acattgtaca ttcttatggc aaggaagcga tgatgttcct
      781 tggagatcat tggatcggaa cagagcccta cggtaaatat ttcgcaggca tcggactgga
      841 tgcagtggta ggctctgtcg gaagcggtgt gacactccga atgatctctg atatcaaggg
      901 agtggattat accgaaggac ggctgctgcc ttactttttc ccggatgtgt tctgcgaggg
      961 cggtgatccg atcggagagg caagggataa ctggcgtaaa gcaaggcgtg cattactcag
     1021 gtctcccctg gacaggatcg gatatggcgg ctatctgaag ctggcaagta attggcccgg
     1081 tttcattgat gaaatacaaa atgtagtaac acagtttcga gagatccatg aaaatatgca
     1141 ggggacagct tcctatgtgg cgcctttcaa ggtggcgatc ctgaactgct ggggcagtca
     1201 gagaaggtgg atgtccaatc aggtacatca tgcgatctgg cacagggaaa cttattccgc
     1261 agagggagta ctggaatgct taagcggaat gccttttgag gtcgagttca tttcctttga
     1321 tgatgtcagg aacggcatcc ctgaggaaat caaggtcatc attaatgtcg gggatgcgta
     1381 cactgcattc agcggcgcgg agaactggat cgacgaaaag gtcctgacca atatcagacg
     1441 ctttgtggat cagggaggtg gcttcatcgg tgtgggtgag cctaccgctt atcagtatca
     1501 gggaagatat ttccaattgt ccgatgtgct cggtgtagac cgcgagatgt gcttctctct
     1561 cagtacggac aaatataatg agaaaaatga cgatcacttt atcttagagg atattgaagg
     1621 agccttggat ttcggcgagg gcaccagccg cgtctatgcg cagggcggac attatcagat
     1681 ccttgcgatg gatggggaat acagccagct ggtagtcaat gaatatggca gaggacacag
     1741 cgtatatttt gcaggtcttc cgtattctcc ccagaactgc aggcttctgc ttcgggcaat
     1801 ttattatgca gccggcatgg agcaggagat gaaacgttac tatgtaacga atgtggatac
     1861 cgaagtaacg gtattccaga agaccggcaa gatcgcagtg atcaacaact ctgcacaggc
     1921 acagcacaca gagctgtata tcaaaggaaa atgtgcgtat gtgctggatc tgaagcccgg
     1981 cgaaatgcgt tgggtggacg atatggaaga caggtaactt atggaaagca aaaagcgttt
     2041 catattttat agggtagtac tttacaagta taaggaggtt atttaagatg aaaaagaaag
     2101 tattaagcgt gcttttagca gcggcaatgg ctatgtccat gctgaccgga tgtggcagca
     2161 agcctgccgg aaatgatgcg cagagcgcag catccagcga ggcatccaag gaaacagagg
     2221 cagcagcaga aacgactgct tccgcagaag acgagacact gaccgtatgg tgttgggatc
     2281 ctaacttcaa tgtctatgca atgaagcagg cagagaaggc atatcaggtg gatcatccta
     2341 acttcaagtt agacattcag gagaaggtat atagcgatat cgagactgcc ctgatcacag
     2401 cggctgaagc cggtgattac agcactttac cggatatctt cctgatgcag gattattcct
     2461 tccacaaaaa tgcaaccaat tatcccggaa tcttcacagc actggatgac tgcggaattg
     2521 atttctccca gttctctatg ggtaagctgg ctgactctac agtaaatgga acacattatg
     2581 gtattccttt tgataatggt gctaccatca tggcgatccg ttccgatatg gtagaagcag
     2641 caggccttaa ggtagaagat ttcaaggata ccacatggtc tgagttcatg gagctggcta
     2701 agaaggtaat ggctgcgaat gatgttccca tgctgacctg ctccggcggt tctgaaattg
     2761 tgatcgagat gttacagtct gcaggcgctt ctcccatggt ggatggaaag gtgacactgg
     2821 ttgacaacgc agcactgaag aaagcaatcg agacctataa gcagctgatc gatgaaggaa
     2881 tcatggcaga ttataccgac tgggatcagt atatcgcttc catgaacaag ggtactgcag
     2941 caggcgttat ccagggctgc tggatcatgt cctccattca ggcagcagag gatcaggcag
     3001 gtaaatgggc aatcgtgaat atgcctaagt tagatgatgt agaaggcgca accaactacg
     3061 ctaactgcgg tggtgcaagt tgggcagtat cttctaactg caaaaatacc gatctggcat
     3121 ttgatttcct gaacgctacc tttggtgcgg atgtagattt atacgatgat ctgctggtaa
     3181 atgcaggagc gatcgcaagt tacctgcccg cagcacagtc tgagacctat aatgagggca
     3241 atgagttcta cggcggtcag gcagtgtaca aggatatcgt agagttcgcc ggacatgttc
     3301 ccggaattga ctacggtgca tactactctg acatcagaag cgcactgaca gatgcaatca
     3361 ccaacgttgt ccagaagaac gctgatatcg atacagagat caaaaacgct caggataccg
     3421 tagggtttaa tatcgctgaa taatcaccag agtatggaaa ctggatagta ccgggggtgc
     3481 agcagtgcgt tcccggtact gttgtaggag agaaacatgg aaaagaagaa agcaaaatta
     3541 agtttggaaa aaaggcataa tctgactgga tgggcattcc tgctcccggc atcgatttta
     3601 atatttgtat tttgtttcta tccgatgtca caggccatcc ttctttcttt ccggaagaca
     3661 ggaggagtct ttaatggtgt agcgaattat gccagaatct tcaaggataa aacattccag
     3721 caatgcttgt tcaatacctt cttttatttt gtgattcagg taccgattat gcttgtgctc
     3781 gcactgatgc tggcgcagct gctgaataat cctaagataa aagggaaagg tattttccgc
     3841 actttgatct ttcttccgtg tgcaacgtca ctggtatctt attccatgat atttaagtca
     3901 ttgtttgctc cggatggagt ggtcaacaga gtgctgatgg ccatcggact gtcttctgtg
     3961 gactggttcc agagtacatg gccggcgcgt tgggtcatcg tgatcgcact gatctggaga
     4021 tggaccggat ataatatggt attctatctg gcaggattac agaatatcga ttattctgta
     4081 tatgaggcaa gctacatcga cggcgcgaca ccgttccagc agtttatgaa gatcacgata
     4141 ccgttgttaa agccgaccat tctgatgacg gcgatcatgt ctacttcggg aacactgcag
     4201 ctgttcgatg agtctatgaa cctgacagca ggcggtcccg gaaaatctac aatgacattg
     4261 gcgcattaca tctacaatat ctcctttgtg gagacaccga aatttaatta tgcggcagcg
     4321 ttgtctgtat gcatacttgt gatggtagcg gttctgtctg caattcagat gaaggtgggt
     4381 gacaaacgtg actaaattca aacaaatgat ttcctacacg atcttagtga tagcaagttt
     4441 tctgtcagca tttccgctgt attatatgct gtgcggcgcg actaaccaga gtattgatgt
     4501 ggtaagagga cgcttgatcc cgggtaccta tatgatcgag aatttcaaga ccctggtcga
     4561 catgcagaat ctcggacttg caatgtttaa ttccttccgc aatgcgatcg tgatcacggt
     4621 tgtgacactg ctgatctgtt ccattgccgg ttatggattt gagatctatc atgataaggg
     4681 caaggatatt ctgatgagta ttctgctgct ggcaatgatg cttccgttcg tagcgatcat
     4741 gatccccctg ttcaagatgt ttacctcctg gaagctggta aatacctgga tcgcactggc
     4801 actgccatcc atctcgactc cgtttatgat catgcttttc agacaggcgg cacgttcttt
     4861 ccctcatgat attatcgagg ctgccagact ggaaggtctg agcgaaatac agattttctt
     4921 ccggatgttc atcccggtga tgaaatctac ctatggagcg gcgatgacgg ttacatttat
     4981 gaatgcgtgg aataattatc tgtggccgac gatcatttta caggacagca aggcgatcac
     5041 catgccgatg ttagtagcca atctgaagag tggatacagt gtggattatg gtatgctgat
     5101 gctgggagta ttgatctgta cacttcctac agcgatcatc ttcctgtgtt tacagaagag
     5161 ctttgcaaat ggaattacgg gagcagtgaa ataatgcaac agaaaatgcc aaaacaggaa
     5221 actatgacac aggcgcatat caggaaggct cagggagcct tccttttggc gcatagaatg
     5281 ggcctgatcg aggatcccac catggagggt ctggagaccc gacgacagaa gcacaatgaa
     5341 gagctgcgga gaatggaaca ggaaggacag cggttctacg gaccgcatta tttttccgca
     5401 ccggcgtatc tgcaatatga gctgacccgc ctgaagctgg attttgtgca gccgtgtgag
     5461 aaggtccggg aaggtggctt ctgcccggat ttcacggagc aggagaaacg ggatttttac
     5521 gaacagaaca gagatctgtt cggacgctat catggggatt attttaccta tgaggaggtg
     5581 tcacagatca ttgagaaacg gttacgggag gatgcatatg acaagcttat ctgcgacata
     5641 ttatgtgagt cagagaacag gcagtgatga aaatgacgga cgcacatgca gtacggcgtt
     5701 tgccagtctg tctgctgtca atcgaagaca cctgcagccg ggcgaccgtg tcctgctgga
     5761 gcggggcagt gtattttacg gacaatatct gcatgtgaca gacagcggta cgaaagaggc
     5821 accgatcgtg atcggagcgt acggcacagc agatgcaccg ccgcggatcg atgcctgcgg
     5881 acaggggatc tggtatcagg attatgggac cccgctggat gcacctacgc atgtgtatca
     5941 cggctatgta tcttccgcag tgcttttgta cgatgcggag catattgtgg tcgaggatct
     6001 ggagatcacg aatgaaggca gcatgatccc gggagagcgt tatagtctgg gtgagaaaat
     6061 gaaccgtacc ggtgtggctg tggtggctaa ggacaaaggg gtgcggggcg gcatcacact
     6121 taggaatctg tggatccatg atgtccatgg gaatgtctac gataaacaca tgaacaacgg
     6181 tgggatctat atgacagcgc tccgtccgga atgtgaggag ctgacgggtg tggcacggta
     6241 tcgggatatc gttattgaag gatgcttcgt gcaccgggtc agccgctggg gaattgcagc
     6301 aggctatacc tatgcccatg ataagttccg gggggcagag cttacggaag ccgtttttct
     6361 gaactatgga catgaaaata tgcagatacg gaataattat gtaaaagagg ccggtgggga
     6421 tgggatcacg ccgatgtatg ccctgcgtcc tttggtggag cataatatgg cagactctgt
     6481 ggcctgcgag atcaatgacc ggatctattg cgagccgggc gaccggatgg gaaaagtggc
     6541 agccgggatc tggccctgga aatgcaagga tgccctgttt cgttataatg aagtcacgga
     6601 caccagactc aatcaggacg gcatggcata tgatgcggat tccggggacg gaacggtgta
     6661 tgaatccaat tacagcaggc agaatgaagg cggctgtgtg atgttctgtc tgcaggaggc
     6721 gatccataat accttccgcg ataatatcag ctatgatgat ctcggtggta cgatcagccc
     6781 gtcagagaat ccggatgccc tgcttcagga taatgtatac tatgtgcgca agggggttcc
     6841 ctttgtacgt aaaaatatgg acggaggcag ctttacacag gtgaatgacc gggttgttga
     6901 gttgtaaaat atggtgctga gcccaggatg aaaacctgtg gcttggcatt tttttaatgc
     6961 ctgatattct ggcggctctg aagccggcag cctgttttta tcgtttttat tgcctgggga
     7021 taaaggattg actgagataa aaaaatacgg tagaatctgt atcaaaaatg atatacgaaa
     7081 atgaggaaag gagccggtgc atgggcgaaa gtatattaca acttcggcag atccggaaat
     7141 catttgacaa tacagaagta ttaaaaggca tcgatctgga ggtggcacag ggagaattta
     7201 ttaccctgtt gggagcttct ggctgcggca agaccacgac gctgcgtatt attgcggggc
     7261 tggaactgcc ggatgccgga caggtgatcc tggaaggaca ggacattacg aaccgtgagc
     7321 cgaacaaacg ggatgtcaat acggtgttcc agaattatgc cctgtttccg catatgaatg
     7381 tgtcggacaa cgtaggatat ggattgaaga tccgcaaagt tccgaaggca gagatcgaga
     7441 gaaaagtaga acaggcactt cgtctggtcc agctggaaga gtacggaaaa cggatgccgg
     7501 atcagctttc cggcggacag aagcagcgca ttgccattgc cagagcggtg atcaatgagc
     7561 cgaaagtatt gctattagat gaacctctgg gagcactgga cctgaagctg cgcaggcaga
     7621 tgcagctgga actgaaacgt ctgcagaagc agctggggat cacgtttatt tacatcactc
     7681 acgatcagga agaagccatc aatatgtcgg accgcatcgg tgtcatgcac gaaggcatcc
     7741 tggaacagct ggggacgccc aatgaggtgt actatcagcc gcagaccagc tatgtggcgg
     7801 attttgtggg aaatgcgaat attctgcata aaaatgggga gacttttgcc atccgctcgg
     7861 aaaacattct gatgaacggg gagactgtct gcacccggga agctgtggtg gtggaaaaaa
     7921 gctttgccgg agggcagtta cgcattttgt tccgtctgcc ggacggacag actataaccg
     7981 ccagccgcta tggaatcgac aatgacttac agccgggaca gaaggtgcag atcggctggg
     8041 atgcgaaaga cgcagtaaag gtaatagata atgacaaaga cgaaaatcac aagtaacagc
     8101 cgcaggagaa gcgggaatgt cggactgatg atccctatgt atgtgttcac gattctgttc
     8161 gtggctctgc caatcctgta tctgttcctt ttgagctttt tacagagagc acaggtctgg
     8221 ggcgtggact ttacattcac cctggacaat tataagagaa tattggaacc gctgtatctg
     8281 gatactttct ggcagtctct gaagctggca tttaccgcca cctttttcgt ggcgctgatc
     8341 ggatatccgt acggctattt catggcaaaa atgaacgctg tctggaagcg caggatgctg
     8401 ttgctcatta tgatcccttt ctggaccagt gcgctgatcc gcctgtacgg ctggattatt
     8461 gtctttcgca gcaatggtgt actggataag atcctgatgg ggctgcacct gacgaagcag
     8521 ccgttaaagc tgctttacac ttatccggca gtggtagtgg gaatggttta ttcgctactg
     8581 ccttttatga tcctggcggt gtattccagt gcggagaagc tggatgcctc tctggtggaa
     8641 gcctccagag atctgggagc ctcggcgtgg aaagccttct ggaccgtcag cttccggctg
     8701 acgctgccgg gacttttgtc cggcgtggtg ctgtccttta ttccgtccat gggactgttt
     8761 ttcatagcgg atatcctggg cggcaataag atcgtgctgg tgggcagtgt gattcaggaa
     8821 cagatcacca agggacggaa tcagcccttt gcggcggcgc tgtctgcggt gttgatgata
     8881 ctgacttcgg tgatgatccg tctgtaccgc aagatcaccg gaacctcaca gctggaaggg
     8941 atgatgtagc gtatgaaaaa gaaaaaatgg ccctttgtat atgtggcggt gatcacagtg
     9001 ctgacctata ttccaatcct gctgacggtg gtgtattcct tcaatgcctc caagctgact
     9061 tccgtgtggg agggcttttc cctgaaatgg taccgggagt tgttccggga cagagatctg
     9121 ggggaagcac tgattaacag cctgatcctt gcggtactta gctgtgcctt tgcagtgttg
     9181 atcggcactt cgggagcact ggggatgagc cgcagaaaac ggaaactgga cgatgccgtg
     9241 gcctatgtgt ccacactccc catcatgatc ccggagatca ttctgggcat ggtatttttg
     9301 tccgtatttt ccctgatggg actgcccttt ggtatggtca cgctggtgat cgcccatacc
     9361 acgttctgtg tgccctatgt gttcactatg gtacgggcga gactggtggg aatggaccgg
     9421 tcgctggaag aggcggcgct ggatctggga gctacgaagt ggcaggtatt tagggatgtg
     9481 atcctgccac agattcttcc ggcgatcctg tcgggagtgc tgcttgcatt tgccatgtcc
     9541 ttcgacgatg tggtgatcag tatttttgtc actggaccga aggtcaatac cctgccggtg
     9601 aagatctaca cgaaattaaa gaccggtgtg acaccggaga ttaatgcact tgccacggtc
     9661 atgctgtttg tgatattgtt gtgtctggcg ggggcgtatg tgctgggatg taagtcgcag
     9721 acagctgtag agaaagctgc cgggggaaaa tgaggaaaga acgattccgc aaatgcaatt
     9781 gcggataagt aacaacataa aaatgtatag cagatgatgc aggagaactg tatcgaagga
     9841 ggagattatg aagaaaaagc tggcaatgct gttggcagcg accatgctgg ccacgttgtt
     9901 taccggctgt ggagcaggcg gtaaagaaga taagggagaa ctgaatctct atacctggga
     9961 cggcatgttc ccccaggaga ttttagatgg ttttgaaaaa gagaccggta tccgtatcaa
    10021 ttattctaat tttgactatg atgaggatat gctggcgaag ctggaagaaa ccaggggcgg
    10081 tgattacgat ctggtcattg cggatgacta tatcattgag ctggctattc aggagagcct
    10141 ggctcagaag ctggatactt ccaggatcag tacttatgat aacctgaatc ctgtatttat
    10201 gtctcagttc tatgacccga agaacgaata taccgtgccc tacggagcgg gcattcctct
    10261 gatcgtatac gatccgggac tgacggatgt gaagatcaca ggctatgagg atctgtggaa
    10321 tccggcactg gaaaataacg tggcactgac ggcaaactat cgtgttatcg acggcatcac
    10381 cttaaagacc atgggagaat cctttaacac cgaggatctg gatgtgatcc gggcagcagg
    10441 agagaagctt ttgagtctgg ctcccaatat ccgtgtgatc aatgacaata atacgcagga
    10501 ttatctgatc agcggtgagg tggcagcagc gttcctgtat acttcccagg tatctgcgac
    10561 actgcaggcg agaccggatc tggaagtggt atatcccaag gagggactgg gcttcggcat
    10621 catggccgga tttatacctt cccaggcacc caatgcagat gcggcttatg catttttgga
    10681 ctatatcaac gatcctgaga atgcggcgaa atgctatgag tatatcggct actactgcac
    10741 caacaaagct gcggaagagt atatttccga cgacatgaag aaaatgatcg tattgccgga
    10801 agatgcagcg gagggcgaga tcgtgcagaa tatctcccag gaggcagagg atctgcatgc
    10861 ggagatctgg aatcagttca agagtgcatg caactaaagt tgcagcaact aaagttgcag
    10921 caactaatct tccgttcagc ccgcgccatt cgcaaagtcg cgttgacacg ctttccgtca
    10981 cttcgtgact acctttgctc ataaaccggc gctcatctca tggacatatt cgttcgaaaa
    11041 atcatgcaag catgattttt gcgctcggat atgtccatgg ctgaactgtt acaataaaaa
    11101 acgcttgaca gatttgattt gtgcgaatat tataaaccta agttgaaaaa ctattttgta
    11161 aatgctatga aagagactct tgccaaggga aacgacagga atacagcgtc accgactgag
    11221 agcgctgttt gcggaagctg gcgaagggaa cactccggag caggtgatct gaactgcaga
    11281 atatcacatg tagctaggat gacacgttgc acgcgttaag tgacaagagt ggataggcaa
    11341 aggcgcctgt caattcgggt ggtaccacgg attagatgat tcgtcccggg atatgtttta
    11401 acatgtcccg gggctttttg tattcaaaaa taagctgtac agggagcgca cattcgggac
    11461 actatataac aggaggtgcg atatgtacag agatttaaca atgaaagaga tcagtgaaca
    11521 gcagatcgga cagactttgc aggtggcagg ctgggtggag aacatccgtg accacggcgg
    11581 cgtatccttt atcgatctgc gggatatgta cggtgtatta caggtggtca tccgtaagcc
    11641 ggagctttta aagggcatca ccaaggaaat gtgcctgtcc ataacaggtc tcatggagca
    11701 cagagacgaa gaaacctaca acccgaagat tccttccggc accatcgaac tggaagcaca
    11761 caagatcgat gtgttgggaa gagtatataa acagcttcct ttcgagatcc agacgtctaa
    11821 agagatccgg gaagatgtga gattaaaata tcgttatctt gaccttagaa atgcaaaagt
    11881 aaaggacaac attgtatttc gttccaaggt gatcagttat ctgcgtcaga agatgacgga
    11941 catgggattt ttggagatcc agaccccgat cctgtgtgct tcttctcccg agggcgccag
    12001 agactatatt gttcccagta gaaaatttaa aggaaaattc tatgcgctgc cccaggcacc
    12061 gcagcaatat aagcagctgc tcatggcttc cggttttgat aaatatttcc agattgcccc
    12121 ctgcttccgc gacgaggatg ccagagcaga cagatctccc ggagagttct atcagctgga
    12181 tttcgagatg agcttcgtga ctcaggagga tgtcttcaag gtcggtgagg aagtattgca
    12241 tgataccttt gtaaagtttg ctcccgaagg cagcaggatc actgagacac cgttccctat
    12301 tatcagttac aaacaggcca tgctggagtt cggctgtgac aagccggatc tgcgcaatcc
    12361 tttgagaatt atggatgtga cggagttctt ccagagatgt acgtttaagc cgtttatcgg
    12421 cagaaccgtc cgtgccatca aggtacatgc ggagatgtcc aaaggcttcc atgagaagct
    12481 gctaagcttt gcaacttctc ttggtatggg tggacttggt tatctcgaag tggcagagga
    12541 tatgagctac aagggaccca tcgataaatt tattcccgaa gagatgaagg gagaactggc
    12601 agaaatggca ggactttctg ccggagatac catcttcttt attgcggaca aagaggataa
    12661 ggcgaactac tatgccggtc atatccgtac tgagctgggc gagaagctgg atctgatcga
    12721 gaagaatgct taccgtttct gctatgtgaa cgatttcccg atgtttgagc tggatcccga
    12781 gacgaagcag atcggattca cccacaatcc gttctctatg ccccagggcg gtctggaagc
    12841 actgaacacc atggatccgc tggagatcct ggcttatcag tacgacatcg tatgcaacgg
    12901 tgtggaactc tcctccggcg cagtacgtaa ccatgacatc gagatcatga aaaaagcttt
    12961 cgccatcgca ggttatgacg aagagacatt aaagaccaag ttcggagcac tgtatcaggc
    13021 attccagttc ggagctcccc ctcatgcggg aatggctccc ggcgtggatc gtatgatcat
    13081 gctgcttcgg aacgaggata acatccgtga ggtgatcgct ttccctatga acggcaatgc
    13141 acaggatctg atgtgcgggg ctcccggcga agtgacggaa cagcagctgc gtgaggtaca
    13201 cattaaagtg agagattagt gcggaaagaa caggaaagga atggaaatta gtatggcgaa
    13261 cgtgatcagt gacgagacca tagagtatgt aggcattctg gcgaagctgg agctctctga
    13321 agaggagaag gaagcagcga aaaaggatat gggaagaatg ctggactata tcgacaaact
    13381 aggcgaactg gatactaccg gtgtggaacc tatgtcccat gtattcccgg ttcagaatgt
    13441 cttccgtgag gatgtggtga ccaatgggga cgagagtgaa cagacactgg ccaatgcacc
    13501 cggcgagaag gacaacatgt ttgtggtacc aaggactttc gattagaaat cgggtagaat
    13561 accgctttat catctggtta cagcatttag gcggtaatgg ttttccgtca gacattaaaa
    13621 aaggagaatg gatatggata ttttatccct gacagcagta gagctggcta aggcgatccg
    13681 ggaaggtaag actacgtcgg tggaagccac gcaggccgtt ctggataaga tcgcagccag
    13741 cgaagatacc taccactgct atgtgaccgt agaacgggaa aaggcattgc agcaggcatc
    13801 ggaagtacag aaaaaaatcg aagcgggcga actgaccggg cctctggccg gggttccctt
    13861 tgctatcaaa gacaatatgt gcaccgaggg aaccctgact acctgctctt ctaaaatctt
    13921 agagaatttt gtgccgacct tcagtgcgga agcagtgctg aatctggaaa aggcaggcgc
    13981 agtgattctg ggcaagacca atatggatga gtttgccatg ggcagcacca cggagacttc
    14041 ttattacgga gtgacaaaga atcccagaaa cccggagcat gtgccaggcg gttcctccgg
    14101 cggctctgca gcggcagtgg cagcggaaga atgctttgca gcactgggtt ccgataccgg
    14161 cggttccatt cgacagcccg catcctattg cggagtcgtc ggcatgaagc ccacctatgg
    14221 aactgtatcc cgttacggac tgattgctta tggttcctct ctggaccaga tcggaccgct
    14281 gacgaaggac gtgacggact gtgccaccgt actggaggcc atcacctccc atgaccctaa
    14341 ggactccact tccatgaagc gggagaacac agatttcacc tccgcactgg tagcggatgt
    14401 gaagggaatg aagattggta ttcccagaga ctatttcggg gaaggactgg atcccgaggt
    14461 cagggatgcg gtactggcag cagcggaagt gttgaaagcg cagggtgcag aagtggagga
    14521 gttcgactta agtctggtgg actatgcgat tcccacttac tatacgatcg cggctgcaga
    14581 ggctagctcc aacctggaac gtttcgatgg tgtaaaatac ggttaccgtg cgcaggatgc
    14641 ggaggatctg cacaccatgt acaagagaag ccgttctcag ggctttggtc ccgaggtaaa
    14701 gcgtcgtatc atgctgggct cctttgtatt aagctccggt tattatgatg cttattacct
    14761 gaaggcactc cgtgtgaaag cattgatcaa gaaggctttt gatgaagctt ttgcaaaata
    14821 cgatgtgatc ttaggaccgg tggcacccac taccgcaccg aagctgggca gcagtctggc
    14881 ggatcccatc aagatgtatt tgggagacat ctacaccatc tccatcaacc tggcgggact
    14941 gcccggtatc agcgttccct gcggtacgga ttcccaaggc ctgcctatcg gcatgcagct
    15001 gatcggtgac tgcttcaaag agaagacact gatccgtgta gcctatacct atgaacagag
    15061 cgtgaagtag aacaacggaa annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15181 nggtctggaa gttcatgtag agcttgccac gaagacgaaa atattctgcg gctgttccac
    15241 agcgttcggt ggcagaccca atacccacac ctgtccggta tgtaccggta tgccgggctc
    15301 tcttcctgtg ctgaacaaac aggtggtgga atatgcggtg gcagtaggcc ttgcgacgaa
    15361 ttgtaccatt acacagtatt gcaaatttga cagaaaaaat tatttctatc ccgataaccc
    15421 tcaaaactat cagatctctc agttgtatct gcccatctgc aggaatggct cggttgaaat
    15481 cgaggtagcg ggagaggatg gcaacactta cacgaagccg gtgcgtatcc acgagatcca
    15541 catggaggag gatgccggta aactggtgca tgacgactgg gaagattgca cactggtgga
    15601 ctacaaccgc tccggtgtgc ctctgatcga gatcgtatcc gagccggata tgcgcagcgc
    15661 ggaggaggtc attgcctatt tggaaaaatt acgtctgctg atccagtatc tgggagcgtc
    15721 cgactgtaag cttcaggaag gctccatgcg tgccgatgtc aatctgtccg tgagagaagt
    15781 gggagcggaa aagttcggaa cccgtaccga gatgaagaac ctgaattcct tcaaggccat
    15841 cgccagagcc attcaggggg agcgggaacg acagatcgat ctgctggagg ccggcaaaga
    15901 ggtcattcag gagaccagac gctgggatga taataaggaa tattcctacg ccatgcgttc
    15961 caaggaggat gcacaggatt atcgttattt tccggatccc gacctggtac cggtctatat
    16021 cagcgatgag tggctggata gcatccgcag cagacagccg gaattccgca ccgagaagat
    16081 gaagcgttac aaggaagaat atgatattcc ggaatatgat atcgatatca tcaccggctc
    16141 caagcatctg gcagatatct ttgaagcttc cgtggcattg ggaagccagc ccaagaaggt
    16201 gtccatctgg ctgatggtgg cgactatgcg cctgataaaa gaaaaagaga tggagccaga
    16261 ggctatccgt ttctccccgg aggatctgag caggctgat
//
LOCUS       DAWDHD010000045        16150 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_243_length_16150_cov_8.821808, whole genome shotgun sequence.
ACCESSION   DAWDHD010000045 DAWDHD010000000
VERSION     DAWDHD010000045.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 16150)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 16150)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16150
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_243_length_16150_cov_8.821808"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         4379 a         3493 c         4716 g         3562 t
ORIGIN      
        1 gggaagtagt accgctatta ggctatggta ttgtggagca gcaggggaca cagaatccgg
       61 aggaggatga gactacctgc cggaccctga ccaccgaaga tctgatcagc gaagaacttc
      121 tccttccgca gccggaggaa acgcagcaga cggcccttat cccggagagt gtggaggcga
      181 cagccgggac tgcattgagc aattttcgtc cggcggtaca acaggtgcag attgaccgca
      241 cacagcttgc ggattatgag acactggtac ggaattttta tgccatagat gccaatacca
      301 tggcaggcag tgatcagtta tccgtggaaa aactgctggg aatggatatg acgttgccac
      361 aggaaggtga cggaccacag atcctgatct atcacaccca ttcccaggag gcttttgcgg
      421 attcggttcc tggagatgtg aacaccggta tcgtgggagt gggagagtgt ctgaccaaga
      481 tcctgacaga acaatatggt taccgggtgc tgcacaatac cggtcaatat gatgtggaga
      541 ccagagacaa tgcgtattcc cgggcacttc cggcagtgga acagatcctt gcggagaatc
      601 cctctatcca ggtgatcatc gatctgcacc gggatgaggt ggcggaagag acaaagctgg
      661 tgacggatat tcaagggaga cccacggcaa gatttatgtt ctttaacggt ctgagcagga
      721 cccgcaagac gggagatatt gactacctgg ccaacgagaa ccaggaggcc aatctggcgt
      781 tttcttttca gatgcagttg aaggcggcgg agtattaccc gggactgacc cgcaggatct
      841 atctgaaggg ctaccgctac aatatgcatc tgcgtccccg gacacttctg gtggaactgg
      901 gagcccagaa caatactgtg gaggaggcca taaatgcctg tgatcctctg gcacatattc
      961 tggatatggt gcttaaggga gagtagcgat acataaaagc aggataagac aacgaatcga
     1021 agtaaaatca tagacaagaa tgcggaattt atgctacaat cgttagatgc aagaatatgt
     1081 caggaaagga cagtagtatg gcagtaatag atcagagcaa aatccgcaat ttttgtattg
     1141 tggcacacat agatcacggc aaatccaccc tggcggaccg tatcattgag aagaccggtc
     1201 tgctcaccag cagagagatg caggcacagg ttctggacaa tatggacctg gagagagagc
     1261 gtggcattac catcaaggca caggccgtgc gtaccgtata taaagcacag gacggagagg
     1321 aatacatttt caacctcatc gatactccgg gccatgtgga tttcaattat gaggtcagcc
     1381 gttctctggc ggcctgcgac ggcgccattc tggtggtgga tgcggcacag ggcattgagg
     1441 cacagacact ggccaatgtc tatctggcac tggatcatga tctggatgtt ttcccggtca
     1501 tcaacaagat cgatctgccc agcgcagatc cccagagagt cattgaagag atcgaggacg
     1561 tcatcggtat cgaggcacag gatgctccgc tgatctccgc gaaaaacggc atcggtattg
     1621 aggaagtctt agagcagatc gtgaaaaaga tcccatcccc caaggggaat ccggacaacc
     1681 ctttacaggc actgatcttt gactctgtat atgattccta taagggtgtt atcattttct
     1741 gccgtgtcat ggaaggcaca gtgaaaaagg gtaccatgat ccgcatgatg gctaccggag
     1801 cgaaggaaga ggtcgtggag gtcggttatt tcggagcggg acagtttatc ccctgtgacg
     1861 agctttctgc cggaatggta ggctacatca cggcttccat caagaatgta aaagatacag
     1921 cagtgggtga taccgtaacg gacgacaaca atccctgcgc agaacctctg cccggttata
     1981 aaaaggtaca gtccatggta tattgtggat tatatcccgc agacggttcc aagtacccgg
     2041 atctgagaga tgcgctggag aagttacagt tgaacgatgc gtccctgttc tatgaaccgg
     2101 agacttctgt ggcactggga tttggcttcc ggtgcggatt tctgggactt ctgcatctgg
     2161 agatcattca ggaacgactg gagagagaat acaatctgga tctggtgact accgcccccg
     2221 gcgttgtcta taaggtgcat aagaccaatg gggaagtaat tgagttgacc aatccttcca
     2281 atctgccgga tccctccgag atcgaatata tggaagaacc ggtggtaaat gcagagatca
     2341 tggtgacttc cgaatttatc ggttccatca tggaactgtg tcaggagcgc cgcggcaaat
     2401 acctcggcat ggaatacatc gagggcacca gagcgttgtt gaaatacgaa ctgcccctga
     2461 acgagatcat ttatgatttc tttgatgctt taaaatcccg ttcccgtggt tacgcatctt
     2521 ttgattacga tctgaagggt tacgagagat cgaatctggt gaagctggac attctgatca
     2581 accgcgagga agtggatgcc ctgtccttca tcgtctgtgc agattccgct tacgagagag
     2641 gacgtaggat gtgtgaaaag ctgaaggacg agattcccag acacctcttc gagattccta
     2701 tccaggcagc catcggcggc aaggtcattg ccagagaaac ggtaaaggcc atgcgtaagg
     2761 atgtgctggc gaaatgttac ggcggtgata ttacccgtaa gaagaagctg ttggagaagc
     2821 agaaagaggg taaaaagcgt atgcgtcagt tcggcaatgt agagatccca cagaaggcat
     2881 ttatgagtgt tctgaagctg gatgataaaa actgatgaag taattgaagg agcaatatga
     2941 ataaaaagga actggaatta tacattcata ttcccttttg cgtaagaaag tgcagctatt
     3001 gtgatttcct gtctgcccct gccacggagc agacaaggga agcctatatg gctgcacttt
     3061 ttgcagagat cgggggaaga gcaaaagatt acagtgaccg taccgtgacc agtatattta
     3121 tcggaggagg tacgccgtcg ttgttgtccg gggaacagat cgggcagttg atggaccgta
     3181 tcagagagca gttcgcaatg gcacaggacg cggagatcac catggaggtg aatccgggga
     3241 cggtcagcgc agagaaactt cggatttttt acacggcagg aatcaaccgt ctcagtatcg
     3301 gtatgcagtc tgcccaggca gaagaactga aaaatctggg caggatccat gattttgaag
     3361 gtttctgcca ggtgtaccgg gaggctgtgg aggcaggatt tacgaatatc aatgtggata
     3421 tcatgagcgg actgccggga cagacgcttg caagctatcg ggacacgctg gaaaaggtgc
     3481 tgcacttaga acccatgccg cagcatatct ctgcctacag cctgatcgtg gaggagggaa
     3541 ccccttttgc ggccatggca gagagggggg aattgccctt gccggaggaa gagacggagc
     3601 gtgccatgta tgaggagacc atagaggtgc tggcaaagta tgggttccat cggtatgaga
     3661 tttccaacta tgccagggat ggttatgaat gcagacataa tgtgggctat tggatccgca
     3721 gggattacct gggattcggt atcggtgcgg catcccttat cgataatgtg cggtttcaga
     3781 acgaaaagag tctgggagct tatctggcac atccgctgga aagcagggaa gaggagcaga
     3841 cactgacggt acaggaccgg atggaggaga ccatgtttct gggactgcgg ctgacaagag
     3901 gagtatcctg tgtggatttt gcagaacaat acggcaggac tgtggaggaa gtctacggag
     3961 aagtgatcgc ccggaatgtg gcggatggtc ttttgaccgt tacggagagg aaagagaatc
     4021 agaaaaaaga ccggctcctg gcactgacaa agagagggct ggatctgagc aattacgtga
     4081 tggcgcagtt tttattctga ggaacgtggg agactgacag gcacttgtgg acagaaaatc
     4141 acataaacta tcataagcaa ctgtccacgg agggattttt tgaagacctt tcagcagcca
     4201 ttaaccgcag gagaagaagc gtattatatc cgcttgctca gggaaggtac cgtggaagaa
     4261 tccagacagg ccagggagat cctggtggag aggaatctgc gcctggtggc acacattgcc
     4321 aaaaaatacc agaatgttga ggaagacatg gaggacctga tctctatcgg atgtatcgga
     4381 ctgatcaaag cagtgaattc ttttgactca ggaaaaggaa gactggctac ctatgcctgc
     4441 agatgcattg ataacgaact tctgatgatg ctgcgcagcc gcaaaaaact ttccagggaa
     4501 gtctccctgt atgaacctat cggacaggat aaggaaggaa atgccatcca tctgttggac
     4561 gtgatcgaag agaagcagaa ggacgtggtg gaggatctgg agcttgacag aaatatcagg
     4621 cgcctgttcc tggctctgga ccactgtctt acggagcggg agtatcggat tctggtgatg
     4681 cgctatggga tcctgggctg taaagaacac acacagcagg aggtgggaga tgttctcgga
     4741 atttccagaa gctatgtctc acgcatggaa aaaagagcat tgcataagct ggcggaggct
     4801 ctgcgtgagg actaaacatc accttgtcaa ttaaggaaca ctgtgatata tttatttcta
     4861 taagtgacta aagataaggg ggggacaggg atgcattttg taagaacgga agacttgaag
     4921 acgggaatgc gtctggcaag acctgtttat aataagaaga gtattctttt gtttgacaga
     4981 aattcccttt tgtcattaca ggcaatagaa agtattcgta atttcggatt gatcggtgtc
     5041 tatgtactgg aaccggcaga accgcttcca cccctgactc aggaggacct ggagttcgaa
     5101 cggtttcaga ttcaggcggt cagtgccatc gaagaggaac aggatcgtat cctgaagacc
     5161 agaaagcaga accgtaccca gtccatcgca gatatggtca tccgcaatta cggacacttg
     5221 gatgagaaaa tcaatttcta ccagaatctg agaagccggg aggattattt ctccagacat
     5281 tccctgaacg tggcaatact ctgtgccatg gttacccatg tgatgaatat cagacgggaa
     5341 gaacagtatc ataccgtatg tgctgccatc cttcacgata tgggaaaagt gaaaaaacat
     5401 gatgacgtgt attacggggc accttatacc agagaggata tgctgcggaa ctgcgagaca
     5461 caggaagagg cctacggtat catcgaggag gcttttgcca ccgacggatc cggcatccgc
     5521 aggatctgcc agcaggcagc cagaaaacag ctggacctgt tcccggaaga cggagagaag
     5581 agcaggtata aaatgctggc cggagccaat gtattgctgg tggcgaaccg ttatgatgag
     5641 atcacggcca tgacattaca gggaacggca cagtctgagg tgaaggctct tcaggaactt
     5701 ctggagcacc cggaggtata tgatccggag acggtacggg cattgatccg ttccatcaat
     5761 atccttccgc ccggtgccag cgtggaactc agtacgggag agaaagcact ggtattgaat
     5821 gaaaatgaag aaagtgtact gcggccgaca gtggtaagct tccgggataa cagtatcctg
     5881 aatctgtccc ttcccgggaa tgaggacatc cagatcgtgg atgtcatgaa gaccatggac
     5941 aaccggtaca tattcaataa atagtgagga gcaattatgc caacaatcga agaaatactc
     6001 agagccgcca aggaagcggg agcaagtgat gtacatctga cggtaggtat cccacccaaa
     6061 atgcgtgtaa acggcaatct gatcaccatg gattatccca ggatgcttcc ggcagacacg
     6121 ctggaagtac tagtcaatat catgacggaa gtgcagagag aacgttttga ggaacgggga
     6181 gaatacgata tgtctttctc tattcccaac tgcggacgtt accgtgtcaa tgcctacaag
     6241 cagagaggtt ccgtggctct ggcgttccga ctggtaggaa ccgtggtgcc ttctccggaa
     6301 gagctgggtg taccggaatc cgtggtggaa ctatatcagc gcaaacgtgg tctggtactg
     6361 gtcaccggac ctaccggaag cggtaaatcc acgacgcttg ccgccatcat cgacaagatc
     6421 aacaacaaca gagatgccca tgtcatcact ctggaggacc ctatcgagta tctgcatcag
     6481 cataagatgt ccatggtcaa ccagcgtgag atcggcatag attccaataa ttatgccaat
     6541 gctttaagag cggcactccg tgaagatccc gatgtcattc tggtcggaga gatgcgtgac
     6601 ttcgaaacca tcagtgtagc tattacggca gcggagaccg gacatctggt attgtccacc
     6661 ctgcatacga tcggtgcagc cagcaccgta gaccgtgtga tcgatgtctt cccaccacat
     6721 cagcagcaac agatccgtgt ccagctgtcc aacacactgg aagcagtcat atcccagcag
     6781 ctgatcccga cagcggacgg caagcaccgt gtggcagctt ttgaggtcat gcatgccaat
     6841 catgcggtcc gcaacctgat ccgtgagggc aaatcccacc agctggccag cgtcatgcag
     6901 accaaccgcc acccgggtct gaccaccatg gacgaggcta ctgttcagct gtattttgaa
     6961 ggcaagaacg acacggaaca tgcgaccaag ctcgcacagg atcccgacag catggaaaat
     7021 aaaatttaaa ggaatgatcc ggaaccggct atataggagc agaccagacg acgcagcgga
     7081 aagcgtacgg ttttgtgaat ctgtagtgag gttccggagg atttttcacc aaaagacgtt
     7141 ttcatttaaa atttctgata ttaattctaa gaagattccg gtagtttcac tgcccttttg
     7201 cgggctctta tgattaccca ttgtatgaat tgttttgtta agtctgatta tttttgtcag
     7261 attcattcgt tttatctgtt tctgttttgt ttcactgtta tttccataat aaataaaaca
     7321 ttttaaagga ggtattttat gtttactaca gtttgttcgg ctgcagtcta tggtctgcag
     7381 gcatatctgg tccgcgtgga agtggatctg gcagggggac taccatcgtt tcagctggtg
     7441 gggtcgctgg ggagtgaggt gaaggaatcc cgggagaggg tgagtgtggc catgaaaaat
     7501 tccggtttcc agatcccacc ggcacatatc acagtaaatc tggctcgggg aggtcgacgt
     7561 atagatggta ccgcctatga tgtgcctata gctgtgggac ttttgcggga tatggaactg
     7621 gtaccggagg aagcgctgca ggatacattg tttttgggag aactgggcct gaatggggag
     7681 atcaagaagg tgaagggtgt acttcccatt gttcaggagg cggcaaaaag cggtatccta
     7741 agggtggtgg tgcccggaga aaatgccatg gaggctgcgg tgataccggg gatcacggtc
     7801 tggggtgtga actgtctgaa cgatctgctg gcggcactga atcatccgga gctggaaact
     7861 gaaaaagtgt atcagccgag gatccgggcg gaggacttgc taagggaagg aaccggacag
     7921 gatacgggag atttccggga agtggcggga caggagaatg ctaaacgggg tgccgagatt
     7981 gctgcggcgg gattccacaa tctgttgatg atagggccgc ccggggtggg aaaaaccatg
     8041 gtcgccgggc gaatccccgg aatcctgccg cctctgacac tgaaagaaag tctggaggtg
     8101 acaggaatct tcagtgtggc gggacttctg ccgccgggac aggcactgat cacaaggagg
     8161 cctttttatg caccacacca tactgtgaca caggcagcac tgatcggcgg gggaagcggc
     8221 atcccaagac ctgggatggt atcgctggcc catcggggtg tactgtttct ggacgagctt
     8281 ccggaattcc ggaaaaacgt gctggatggc atgcgacagc ctatggagga gcgtaaagtg
     8341 cagatcgcca gaagtgccgg gatggtcact tatccgtcgg actttattct ggtggcggct
     8401 atgaatccct gtccctgcgg gtattatccg gaccggaata aatgccgctg tacccagccc
     8461 cagatcgaga aatatctggg aaagatatcg ggaccgattt tagaccggat tgatctgtgt
     8521 gtggaactgc atcccgtgga tattctgcat ctgcagaaaa cgaaaacgga aaaaagcagt
     8581 gaagatatga agcggcagat ccggcatgca agagaacggc aggcggagcg ttttgcagga
     8641 acggattgcc gtttcaatgg ggacatccgg tctgcacagg tggaagaata ttgcccgttg
     8701 ggagaggagg gtcagaaagt catggaacag ctgtatcgta cgctgcagct cagtgccaga
     8761 gtttatcacc ggattctcaa aactgcccga accatagctg atttggagga ccgggaagag
     8821 atcacggggg aacatttgct ggaggcggcg tgttatcgcc cttcggagac gtattggaat
     8881 taattgtgtg ccggaaccgg gtacattaag tgtccggcaa gaaaaagggg ccggtacgaa
     8941 aatcacggaa aacgtgcggg ctgtgtgaaa acagggaaag ggacaggtat gtttatggcg
     9001 gaggaagaga gaaaatatgc atattttttg tgcagcacct atacaatcgg gggcgtgcgg
     9061 gcagaacggc tgcttgcacg cttcgggtca ccccgggcgg tatatgaggc ggaggcagag
     9121 gaatggaggg agtgcgtggg caattctgcg gcggaagcct tggaacggca gaagaaaagc
     9181 ggagagtggg aacgggaata tggacagctt tcggaacaac agatccactt tcttttgcgg
     9241 gaggagaaag gatttcccgg gaaactggca gaaattccgg atccgcctta cggcattttt
     9301 taccggggaa aactgccgga tgagaacgaa ccggcggtgg cggtcatcgg tgccagagag
     9361 tgttcggaat acggcagata tgtggccgag gaactgggac agtatctggg aagagcggga
     9421 atacaggtca tcagcggaat ggcccggggg attgacggga tcagccagca ggcagcgtta
     9481 tctgcgggtg gaacttccta tggtgtgctg ggatgcggag tggatatctg ttatcccgca
     9541 cagaatcgaa gattgtatga agaactgttg gaaaaagggg gattgctttc cacctatgcg
     9601 ccgggaacaa agccgctgcc ccagtatttt ccaccaagaa accgtattgt cagcggactg
     9661 tcggatgctc tgatcgtcat tgaggccaga cagaagagcg ggacgttgat cacggtggat
     9721 atggcactgg aacagggcag ggacgtctat gcggtgccgg gcaggcttac ggacaggctc
     9781 agcgacggct gcaatcatct gctgacgcag ggagcgggag tgtttttatc tccggagagt
     9841 tttgcagagg attttttgcg gagctgggag gaaaaacagg gactttccgc agatggctcc
     9901 ggaggaaaga acaggcggaa aaataatagg gaaaccggca gaaatacggc aaaagtaaaa
     9961 ttgcccaaag aatccctgca aaagatcttg gaatgtctgg aacctgtgcc aaagacatcg
    10021 gagacgctgt tgcaggaact ttgggagaag ggagaatata cggacatttc cgcactgaac
    10081 cgggcactga tggaactgga acttttggga tatgccagac agaacagtcc gggacatttc
    10141 agcacttctg cgaattgaca aggctttcgg aaaaggggta tagtaataga catatgagaa
    10201 tgacgagaaa ggtcaggtgc ccttatgaac gataaagata cttacagaat gcataccaaa
    10261 gtaatacaga tcggagaccg gaagatcggc ggcggcaatt ctatcgccat ccagtccatg
    10321 accaatacga agaccgagga tgtgaaggca accgtagagc agatcctgcg tctggagagg
    10381 gcaggctgtg agatcatccg ctgcacagta ccgacgctgg aggcggcagc agctatccgg
    10441 gagatcaaga agcagatcca cattccttta gtggcggata tccattttga ctaccgtatg
    10501 gcactggcag ccattgagaa cggtgccgac aagatccgca tcaatcctgg gaatatcgga
    10561 aatccggagc gggtaaaggc tgtggtagat gcagcaaaag agaggaatat cccgatccgt
    10621 gtgggagtga acagcggttc cctggagaag ccgctggtgg aaaaatacgg tggagtgact
    10681 gcggaaggta ttgtggagag cgcactggac aaggtacaca tgatcgaaga tctgggatat
    10741 gacaatctgg tcatcagtat caaatcttcg gatgtgctga tgtgcgtgaa agcccatgaa
    10801 ctgctggcag gccgtacggt gtaccctctg catgtgggca ttaccgaatc cggtactgtg
    10861 aacagcggta atatcaagtc agccatcggt ctgtccctga tcttaagcca gggcatcggt
    10921 gacaccatcc gggtatccct gacaggagat cccgtagagg agatcaaatc cgcaaagctg
    10981 atcctgcgta cattaggatt gcgcaagggc ggcattgagg tcgtatcctg tcccacctgt
    11041 ggaagaaccc agatcaatct gatcgatctg gccactaaag tggagaaaat ggtagaggat
    11101 tacccgctgg atatcaaagt ggctgtcatg ggctgcgtgg taaacggccc cggagaagca
    11161 aaagaagcgg atctgggagt tgcgggcggt atcggagaag gtctgctgat caagcatggc
    11221 gagatcgtaa aaaagcttcc ggaggatcag ctgctccccg ccttgaaaga ggaattggat
    11281 cattggagtt aaaataaatg agtaaaccgt tttttgaggt atttccttcg ttacagttga
    11341 atacggacat tcgtgacctg atggggcaga cagaagtgga gagagtctcg gcgacaaaac
    11401 gcagagactt tcttcgggtg tacttaaaaa gtacccgcct gatccagaaa gcggacatct
    11461 ggaccacgga acaggagatc aagaagcagc tgttccctca ggccaacctg acagtgaaaa
    11521 tatacgaaaa gttcgaattg tcttcccagt acaatccgga aaagctgatg gatatttata
    11581 aggaaagtat tctggaggaa ttccgggagt acagccatat ccagtataat gccctgaaga
    11641 cggcgaaaat cgagtatcct tcggaaaatg aaatgctttt aacgctggaa gatacggtat
    11701 taaatcacag tatggaaggg gagatcctgc agattctgga gaagatcctg gtggaacgtt
    11761 gtggattctc cgtgaagatc catacggctt atgtggaaaa agagaccggg cgtttccagg
    11821 aggaagagga agcaaagatc cgtctgaagg tggatgccat ttacagtcgt acgaaaggca
    11881 gaagagaaga aggagagcag actgcatcgg cggaaggaag cgggcaaagt acagcacagg
    11941 atccggaaac cggcaaggct gcagagaccg cacaaaagcc gaagaccgag aatgccggtg
    12001 gcgtgaccaa gagctttcag ggtggcagcc gcggtgaatt caagcgtgga gaattcaaaa
    12061 agggcgagtt tcgtaagggt ggtaactttg aaaaaggtgc gttgaagaga tcagacaatc
    12121 cggatgtcat ctacgggcgt gatttcgaag aagaggccat gaagattgag gaactcatcg
    12181 gagagatggg tgaggtggtc atccgcggta aggtactgtc cgtggatacc cgtgacatca
    12241 agaacgagaa gacgattatt attttcaata tctctgattt taccgatact atgaccatca
    12301 agatgttcgt gcgtaccgag caggtgaagg aagtgaccgg ggacatcaag ccgggagctt
    12361 tcttaaaggt caagggaatc tgtatgatgg ataagttcga tcacgagctg gccatcggtt
    12421 ccattgcggg aatcaagaag atccctgatt ttaccaatac gagaatggat acctccgcca
    12481 gaaagcgtgt ggagctgcac tgtcatacaa agatgagtga tatggacggt gtctccgagg
    12541 ccaaggacat tgtaaagcgc gcctacaaat ggggacatcg ggccattgcc atcacggatc
    12601 acggcgtggt acagagtttt accgatgcaa accatgtctg ggatgacctg tggaaagcgg
    12661 aaaaaggcaa gcggaaagaa gccggagacg agaatccgga caagcaggat ttcttcaaga
    12721 tcatttacgg tgtggaagcc tatctggtgg atgatctgaa agagatagtg acgaatgata
    12781 agggacagag tctgcatgag gattatgtgg tattcgatat cgagaccacg ggattttccc
    12841 cggtcaacaa ccgcattatc gagatcggtg cggtaaaggt atccggtgga gagatcgtgg
    12901 atcgtttttc cacctttgtc aacccggatg tgccgattcc cttcgaaatc gagaagctga
    12961 ccagcatccg ggacgaggat gttatggatt ccccccagat cgacgtgatc ctgccgcagt
    13021 ttctgcagtt ctgtgaaggc tgtatcatgg tggctcacaa tgccagcttt gatatgagtt
    13081 tcattatgga gaattgcaga agactgggct atccgcagga gtttacctat gtggataccg
    13141 tgggcatttc cagagtactt ttgaagaacc agtcgaagca tacgctggat gcggtggcca
    13201 agaccctggg gatttccctg gagaatcatc acagagccgt ggatgatgcg gagtgcacgg
    13261 cccatatttt cgtgaaattt attaaaatgc tggaagagca ggacattcat aatctgacgg
    13321 aagtcaatgc actgggagct tccagtgtgg atgccgtgaa gaagatgcct tcttaccatg
    13381 ccattattct ggcgaaaaat gatctggggc gtatcaacct gtatcgtctg gtgtcccagt
    13441 cacatctgac ctattttaac aaacatcccc ggattcccaa gagtctggtg atgaagtaca
    13501 gagaaggact gatactggga agtgcctgcg aggcgggaga actgtaccgg gcactgttgg
    13561 acggacagtc ggatgcccag attgccagac tggtgaaatt ctatgactat ctggagatcc
    13621 agccctgcgg caacaacaaa ttcatgatcg cttcggaacg tgtgaggaat gtaaattcca
    13681 ttgaggatat tcagaatatc aaccggaaga tcgtgtccct gggagaacag ttccacaagc
    13741 cggtagtggc tacctgcgat gtgcatttcc tggatccaga ggatgaggtc taccgccgta
    13801 tcatcatggc gggacgagga ttcgatgatg cggatgatca ggcaccattg tatttacata
    13861 ccacggaaga gatgctggaa gaattctctt atctgggaag tgataaggcg gaagaaatcg
    13921 ttattaccaa taccaatatg attgcagaca tgatcgagac tattgcaccg gtgcgcccgg
    13981 acaaatgtcc tccggtcatt cccgacagtg ataagacact gacggagatc tgttataaca
    14041 gagcccatga aatttacgga ccagatctgc ctcagatcgt agaggcgaga ctggaaaagg
    14101 aactgaattc cattatcaag aacggattcg ccgtaatgta tatcattgcc cagaagctgg
    14161 tatggaagtc ggtagaggac ggttatctgg taggttcccg aggatctgta ggaagttcct
    14221 tcgtggcgac catggcggga atcacggagg tcaacccctt aagccctcat tattattgca
    14281 gtaagtgtca ttatgtggat tttgacagtg atgaagtcaa ggcctatgcc ggtaaggcgg
    14341 gaatcgatat gccggacaag ctgtgtccgg tgtgcggaga aaagcttcac aaggacggat
    14401 ttgatattcc tttcgagact ttcctgggat tcaagggaga caaagagccg gatatcgacc
    14461 tgaacttctc cggtgagtac cagtccaagg cgcataaata taccgaggtt atcttcggtg
    14521 cgggacagac cttccgtgcc ggaaccattg caactctggc agataagacg gccttcggtt
    14581 acgtgaagaa ttattttgaa gaacgtggca ttcacaagcg gaccagtgag attgaccgta
    14641 ttgcttccgg atgtaccgga gtacgacgca gtaccggtca gcatcccggc ggaatcgtcg
    14701 tactgccctt aggacaggat atcaactcct tcaccccggt gcagcatccc gcaaatgata
    14761 tgactacgga taccataacc acacactttg attaccattc cattgaccat aacctgttga
    14821 agctggatat tctgggacac gatgatccta ccatgatccg tatgcttcag gacctgacag
    14881 ggagagatcc tctgacgatt cccttagacg gacaggatgt catgagtctg ttccagaata
    14941 cggatgcgct gggcattact ccggagcaga tcggcggaac aaagctgggg gcattgggta
    15001 tcccggagtt tggtaccgat ttcgccatgc agatggttat cgatgccaag cccaaggcat
    15061 tttccgatct ggtgcgtatt tccggcctgt cccacggtac caacgtatgg ctgggaaatg
    15121 cacaggatct gatcatgagc ggtgtggcaa cgatttccac cgccatctgt acccgtgacg
    15181 atatcatgct gtatctgatc cagatgggtg tggaatcgga gaagtccttc aagatcatgg
    15241 aggccgtgcg taaaggcatg gtggcgaagg gcagatgcgc catgtgggaa gagtggaaag
    15301 aagacatgct ggcccacaat gtaccccagt ggtatattga atcctgtcag aagatcgagt
    15361 acatgttccc gaaggcccat gcggcggcat acgttatgat ggcatggcgt attgcatatt
    15421 gtaagatcca ttatccgtta gcctattacg gagcgttttt cagtattcgt gccaaggcat
    15481 tttcttatga aattatgtgt cagggacaga agcatttaga gagcgttatg gtggattaca
    15541 aaaagcgaca ggatacgctt tccaataagg agaaggatgc catgggtgat atgcgtatcg
    15601 tgcaggaaat gtatgcacga ggctttgaat tcgagccaat tgatatcttt agagcccagt
    15661 ccagatcctt ccagatcgtg gatggcaaat taatgccttc cttaaacagt atcgacggac
    15721 tgggagagaa ggcggcggat gccatcgtgg aagcggcaaa agacggcccc ttcctgtcta
    15781 aagatgattt ccgccagaga accaaagtct ccaagaccat catcgacctg atggattccc
    15841 tgggattatt gggagacctc ccggagtcca atcagatcag tatatttgac ctggtgtaac
    15901 agctcagcca tgggacagcc gaaacggggg cgtgggtgct tgcacacaaa gcaccgtttt
    15961 ggctagccca tgagcggagc gcccaatcca tgaagcaaaa gatgagccgc gcaagcggcg
    16021 tcgactgcgt agcagccgct tttgtgaatg gccgtgggct gagctgtagt ccggccagcc
    16081 gcttttatga attgaccgtg ggctgagctg tagtccggcc agacgcggtt gggtatgggg
    16141 gggggctgag
//
LOCUS       DAWDHD010000046        16101 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_245_length_16101_cov_11.316154, whole genome shotgun sequence.
ACCESSION   DAWDHD010000046 DAWDHD010000000
VERSION     DAWDHD010000046.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 16101)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 16101)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16101
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_245_length_16101_cov_11.316154"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3924 a         4413 c         3603 g         4161 t
ORIGIN      
        1 ttttttctct gctgagcccc ggaagtttcc gacaagcggg gcgtaattta catttttctt
       61 ctactttgtc ccgaagataa aaccctccgc aatgagctac gctattcata agattgtcag
      121 cataaagcgc tgccaatctt atttcatatc gcggcgttcg ccacatctcg gcataagtaa
      181 agacagcgaa tcatgcgcga gcgcccatcg ctgtcttcac tatgcctatg cgcggctcat
      241 tgctcgcgta actttctatt taccgaaact tccccgccca atattcgtct tattataatg
      301 tgatcaccat aacacttcac gattttggga gggtatcttt atgaagaagg ctattttact
      361 atcaacactg acaacgattc ttacgctttc cttggcagga tgtggttcta ccgggggcct
      421 ggaggattct gtaggggtgc aaaattccac ggcccccacc gggccaatcg ccccggtcat
      481 cgcggaaagc ctcgttccta cagaatgccc ggaaaacggg cctgccgacg atctggcgat
      541 atccatgggg aatgaagcta tctgtgatct gggaccggtg cagttctccc tgtcccttcc
      601 ggagacctgg gactatgaga tccgcaattt aactttcgca ggtaccacca cgaccgatgc
      661 tatcatcttc tggaacatcg catacccgga ggataccttt acgcttgtct atgctcccgt
      721 cccgggaatc tgcggcaccg gcgtcaccag cgaggagatc actctgcccg acagcaactt
      781 aaacggctgg aaaaataccg agatctacca gacggaacaa aaatactgga tttacattac
      841 attatccaat cccgatgtcc ctttacaggg aaatacccta cagctggaag ccactattcc
      901 gttaacagac tggtacactc tggaaacaga tttcaaccgc atcctcgaaa ccatccagct
      961 tcaatataca agtttcgaag ctcctgtgac gcaggatatt ctgatcttgc cggaagaaac
     1021 gcagtcaacg actccagaca ttctgccctt gccagaagaa acgcagttcg ctccccaaga
     1081 caagttgccc ttgttagaag aaagacagtc caactgtatc gagctggata cggatttcgg
     1141 tgcacctgtg actcaggata ttctgatcct gccagaacaa actcaacctg caactcagga
     1201 gaaactgccc ctgttggaac aaaggcagtc caactgtatt gagttggatg cagatttcag
     1261 agcccccgca tcgtaggata ctctaatctt accgaaagaa tcgcatcctt gtctcacgac
     1321 agccgccatc cttggccgcc agataaggga atctccacag attgcccaca cccaccgcgc
     1381 ttccggccgc cgccagcacg aaaccgatct gcccggaaaa attacttgtt ttcttttcca
     1441 tatgattcac gctccttaaa acacttgtga catgaggata gcatatgcta tactatcagt
     1501 aaaacgaatg tttttactag tatgcataag tatttgtaat gtgatgagtc tcaagagaaa
     1561 acaagtggct gcaaagcaga catttgtttt tctggactca tcagctcacg aatatcctgc
     1621 gaagcaggat gtaaagtgcg aagcacgaat tcgtgagctt atactataat aagtacaaga
     1681 aagccaatct gctcgcataa ttggtgaacg atgaatttga aattcataaa tttacggggt
     1741 gaagatcatg aaagaacaaa aagaccgtta cgagatattt ttaaaagtat gtgaaacggg
     1801 aagcttctcc aaggcggcgg aagcactgaa ttatacccag tccggcatca gccagatgat
     1861 ggcgggactg gaggaagaac tgggtgtgca gctttttgcc cgggttaaac ggggtgtcag
     1921 cctcaccgac aacggtgccc gcctcatccc ttacattcag gaaatggtca accagaaaga
     1981 caagctgcgc caggccgcct tcaatatcaa caataagatt gagggaaaac tgcgggtcgg
     2041 tagtttttcc agcatcaccg ctctgtggat gccggatgtg gttcattttt tccgggaaaa
     2101 ttatccgaaa gtggatgtag aaatttttga cggcaattat gatgagatcc gggactggat
     2161 cattcacggg caggtggatt gtggattttt atcctccatc gtggcggatg atctgacctt
     2221 ttatccactg ttgaatgatc cgctgtgcgc tgttttcccc gagggacatc cgctggctgc
     2281 caaaaacagc gtcaccctgc cggaactgtt cgaatatcct ctgatcatcg agacccccgg
     2341 ctgtgacaac gacatccagc atctgctgac caaatgccct gtccgcccaa agatctctta
     2401 cagcttccgg gacgataccc tgatcatgtc cttcgtccgg aacggtctcg gtgtcaccat
     2461 ctctcaagaa ctggtattaa aagctttcgc acggggtgtg gtctccagac ccttatctcc
     2521 cagttgtcac cgtacgctgg gactggcttt ctccaagacc gccaactccg tggtgtcaca
     2581 gacgatgctg cggtatttgc aggaaacgat ccactgatcc accccagctc aactattctg
     2641 ctattctaca ttatgatagc ttccgcaata tgggcagcgt ccggtatatc ctgctgccgc
     2701 ctgataggaa gcaccgcagt tcggacactg cacggcggtc atgcgaagct tggaaggatc
     2761 cacgggctta tcgtccatca gttcctgctc tcccggcaga atctcatccc ggacggaatc
     2821 cggatcccaa ccgtattttc cctgatattc gtagatatcc atggtcatac cgatgggata
     2881 ggtcgaagcg cccttttcaa acgcagtggg aatcactgcc tcccgctcga tctgattctt
     2941 gtcaaaataa tgcacgatca cattcaccgg gaatctgccg tttaccatat acgccgtatt
     3001 ctccacgtaa ccgtagatct tggcggcata tctgattccg ttttttacga tgagtgactt
     3061 tttatgctgc ccgaataata ctgctgcgat aaaacaaatg cccagtatca ggaaaaacag
     3121 cggcaatgcc agaaatgccc tcatgggccg gaacaccatg ccgatgcttc cgaatatcat
     3181 tgtcagcact gcgccgatcc ccgcaaatgc gcctcctatg atcagtaaaa ctttctgagc
     3241 catgttcatt ttcatgtcag tttcccgccc ttctcctatt tttcatgcca atctccatcc
     3301 agcagccttc ggctggcggc acaaatagta atttatacca tctggaccac ccagacatta
     3361 ctcttcacca tgaggtaacc aatgatcacc ttgatcagtt ccgtaccctg caccagaaaa
     3421 tagatgctca cgatccccag gccggtgaaa tgcgccagca gatatgccgc cggcaccacg
     3481 atcacccagg taaatacgga atcaaacagg aaagtcacca ccgtcttacc accggaacgc
     3541 agggtaaaat acgacgcatg gctgaaggcg cagaagggca tgatgatggc cgataccgca
     3601 ataaaactgg tagccagttc cctgatctcc tccgtggtat tgtaaatacc cgggaaaaat
     3661 cctcctacca cgaacatcaa tgccgccatg atcacacagc agaagacgct gaatacgatc
     3721 atcttgttgt ctgcatcctt ggcatcctcc aatttacctg cacccagata ctgtcccacc
     3781 acaatactga tacaggaacc cagctgaaga tagacaatat tgaacagatt cgtgatggtg
     3841 gaggcaatgt tcagtcccgc caccacgtcc agacctctga cagaatagca ctgggtcacc
     3901 gttgtcatgc ccgctgccca cagcacctcg ttcatcatca gcggaaatcc cttgatcagg
     3961 atcgccttca attccgcaag cggaatacca aagccggtgt aagctccctc cagatagcgg
     4021 ttttttcccc gatggatgtg tgcccagatc accacgatca gtgcctcaat gtaacgggca
     4081 ataacggtag ccagcgccgc cccctgcact cccagctccg ggaaaggacc gatgccgaag
     4141 atcatcagat agtccagaat cgcattagta cccaccgcca caaagctggc cagcatcggg
     4201 ataaaggtct gcccggtctc ttttatattt gtcgcgtagg actggttcac cgcaaaaggg
     4261 atcagtccca ccatcatgat ggccagatat tccagtccgt actgcagtgc caagtccgtg
     4321 gccccgttcc ccaccgtatc cgtcagatac agggagatca gtgcggaacc tttcgtcagg
     4381 aacagaagga tcgctccgcc tgtcaccaac agcgtggcat acagcttaaa ccgaaaggaa
     4441 tacatatgtc ccttatgatt gccctttccg aaatactggg caccgaagat gctggcagct
     4501 gacacagctc cgaagattgc cagattgtat acaaagatca gctgattcac aatggcaacg
     4561 ccggacatct gctccgttcc aagctgtccc accatgatgt tatccagaaa gcttaccaga
     4621 ttggtaatgg cattctgcag gatcatggga acagccagta aaagatactt ctggtaaaaa
     4681 tcccagtctc caatatattt tttcttaaat tttgctaaca taatttccct caattttcta
     4741 aataatccct acatacgctc gatcatcccg ggactttcac tcccgaaaac cgaccgctta
     4801 taacggttct tcctgcggca tggtgctgtg tactttcttt ctgacaaaat agaaacatac
     4861 cgcctgcagc aggatcgttg ccagccagga ggccggataa gataaaaaca gtaccatcaa
     4921 cgaccggtgc gttgggaata cttcgtagat ccagaagatt ctcgttccca ccgtaccgat
     4981 aatggacaat atcataggta ccgccgaatg tcccatgccc cgcaaggctc ccggaatcag
     5041 atccatgatg ccgcataaaa agtaagtcaa cgtcgtatac agcagaatct ccgttccaca
     5101 ggcaataacg tccgcatccg ttgtgtaaat gtgaagcagc tgcggaccga agagattggc
     5161 gcttccaccc agcgttaccg ctacgatcac cgacaggatc atacaatcga tcagtaccct
     5221 gtccatacgt tttttcttcc cggcaccgta gttctgactg gtgaagctca tacacgcctg
     5281 ggtaatggaa ttcaccgaca cgaacaggaa tccaaagata ttattggccg ccgtatagcc
     5341 tgccatggcg atggagccaa aggaattcac cgaggactgc agcagaacat tggagaaatt
     5401 gatgacaacg ctctggatcc ccgccggaac ccccacctga aagatctgca gcaggtactc
     5461 cccttttatt cccagctttg aaatctgcag ccggtaacag ctgtccgtag taaacaggca
     5521 ccataatacc agcacacagg agatcacctg agagatcact gtagcaatgg ctacccccgc
     5581 cacacccatg gaaaaagcaa tgaccagaaa caggttcaat accgcattga tggctccgga
     5641 tatgatcagg aacaacagcg gacgcttcgt gtctcccact gcccgcagaa ctgcagctcc
     5701 gtaattgtac agcatgaaaa agggcatgct gcagaaataa attttcatat ataatgccgc
     5761 ctgatcaata acatccgccg gggtatccat cagttccaac gctccccggg tgcagaacag
     5821 ccctacgaat accatgacaa taccgctgat caatgcaaag gtcatagctg tatgtactgt
     5881 ctcgctcatc tcccgctctt tcccggcagc atagaatctg gcggacaata cattggctcc
     5941 cagagaaacg ccgataaaca gattgacgaa catattgatc aatgccgtgg tggaacctac
     6001 agccgccagc gcctgacttc cggtgaaccg tcccaccaca atgatatcca ctgcattgaa
     6061 cagcagctgc aaaatgctgg acaccatcag gggcagtgca aaggagatca gcttgtccat
     6121 aatcgtgcca ttgcacatat ctatttcata tttgtttttc ttctgtgttg cttccataat
     6181 ttcatttcct gtaattctct tccaaatccc gttattttcc cacaccgctc atccgcatct
     6241 gcagaagaac cgctataact tatgattcta tcacatattt tgctatattt ctattcccct
     6301 ttttatctaa aaatatggta tactatattt tgaatgtttt tggtttttca aagtgatgat
     6361 acatcccgca aatccttaca agagagcaaa gagaaaggaa acttatccct atgaaatatg
     6421 acgtaatcat tatcggtgcc ggccccggcg gtatcttctc cgcttatgaa ctggtgcaac
     6481 agaatcccga cctgaaaatt gccgttttcg aatccggtca ccctctggag aagcgccact
     6541 gtcctatcga cggtgacaag atcaagagtt gcatcaactg taagagctgt tccatcatga
     6601 gcggtttcgg tggtgccggt gctttttccg atggaaaata caatatcacc aacgctttcg
     6661 gtggcactct ctacgagtat atcggcagga gtcaggccat cgacctgatg aaatatgtgg
     6721 atgacatcaa catgaagttc ggcggtgaag gcacgaagct gtattccact gctggcacct
     6781 ctttcaaaaa gatctgcatg cagaacaagc tgaacctgtt agacgcttcc gtacgtcacc
     6841 tgggtacaga tatcaacttc atcgtgctgg aaaatctgta cgcagaactg aaggataaag
     6901 tgactttcta tttcgatacc ccggtgaaaa cggtggagac cgttggtgac ggttaccgta
     6961 tctgctgcga aaacgcttct tacgactgcg acaagtgcat tatttccgta ggccgcagcg
     7021 gcagcaaatg gatggagcag atctgtaaag atctggacat caacaccaag tccaaccgcg
     7081 tggatatcgg cgtccgggtg gaactgcccg cagtcatctt ctctcatctg accgatgaac
     7141 tctatgagag caagatcgta taccgcactg aaaaattcga ggattccgtg cgtaccttct
     7201 gtatgaatcc ccacggtatc gtcgtaaatg agaacaccaa cggcatcgtc accgtaaacg
     7261 gccacagcta cgaaggagcc gacaagcaga cagagaacac caactttgcc ctgctggtag
     7321 cgaagcattt ctccgagccc ttcaaggaca gcaacggcta cggtgaaagt atcgcccgtc
     7381 tctccaacat gctgggcggc ggtgttatcg tacagcgttt cggcgatctg gtgagaggac
     7441 gccgcagtaa tgcgaagcgt attgccgagg gcatagtaga gcctaccctg tccgcaactc
     7501 ccggtgattt aagcctggta ctgcccaagc gtatcttaga cggtatcatc gaaatgatct
     7561 acgcactgga caagatcgca cccggtaccg ccaatgacga taccctgctc tacggtgtgg
     7621 aagtcaaatt ctataatatg gaagtggaac tggacgaaca tctggaaagc tgtcacaagg
     7681 gcctgtacat catcggtgac ggcagcggtg tgactcattc cctgtcccat gcatccgcca
     7741 gcggtgtcta tgtcgcaagg cagatcgcca aaggggaata attactaata aaaaggaagg
     7801 tagattctat ggcacagaca gttaagatca tctctgacag tacctgtgat ctgtcaaagg
     7861 aattagtgga aaagtatgac attaccatcc tgcctctgca cattctgctg ggcgaggatg
     7921 aataccgcga tggcgtggat attacgccgg aagaaatttt ccggtggtcc gatgaaaaca
     7981 agaccacgcc caagacctct gctccttctc tgaccgaggc catggaagtc atgcagcctt
     8041 ttgtggaaga aggccgggag atcatctgtt tctccatctc cgacagtatg tccacctccg
     8101 gcaacgttat gcggcttgct gcagaagaac tggaagcttc cgacaaagtc accgtcatca
     8161 actccgcaaa cctgtccacc ggcatcggtc tgcaggtaat acaggctgcc attctggctt
     8221 ctgaagggaa aacagccgca gagatcacta ctgccatcga agccatccgt cccaaagtcc
     8281 gggccagctt cgtcgtagat accctgacct accttcaccg cggtggcaga tgcagcgcgg
     8341 tagcggctct ggccggtggt atgttaaagc ttcatcctcg gatcgtcgtg gtggatggtg
     8401 ccatggatgc ctccaaaaaa taccgtggaa aactgggttc cgtcatcctc tcctacacaa
     8461 aggatatgga agaggatctg aagaatgccg ttcccgacag agtcttcatc acccactccg
     8521 gctgcgaccg ggagattgtg gaagcggttc gttcctatct ggaaagtctg ggaatctttg
     8581 acgagattct ggaaaccaga gcaggcggtg tagtctccag ccattgtggt cccggcacgt
     8641 taggcgtact tttcattgct aaataaaaaa tccgaaggag tttcgtgtga aaaattaatt
     8701 tttcacacgc aaatttgtgc ctgcacaagg tgccaaggca cctgaaagtt tgcaggttac
     8761 tgaaaggcat tggtattatt atgatccgtt ttatattcgt cgtattattt gtagtattat
     8821 tcctgatcct gtccacgccg ttaatgctgg tggaatggat catcggcaaa tttaaccccg
     8881 gtcttaagga ccgcagttcc ctggccattg taaactgggc cttccgctgg gtcatccgtc
     8941 tttccggcac gaaagtcatt gccatcgggg aagagaacat tcccaccgac actgctgtgc
     9001 tgtacgtcgg caatcatcgc agctttttcg atgtggtgct gacctatgtc cgtgttccca
     9061 gacctaccgg ttatattgcg aaaaaagaaa tgttaaaatg gcccctgctt aacatctgga
     9121 tgaaaaatct tcactgtctg tttctggatc gccaggacat caaggcagga ctgaaaacca
     9181 ttctgcaggc cattgagaaa gccaaaaacg ggatttccat ctgcatcttc ccggagggca
     9241 cccgcaacaa gacccaggat accttccttc ccttccacga gggcagtttc aagatcgcag
     9301 agaaagcaaa tgttcccatt atccccatga ctattgtgaa ttccgctgcg gtatttgaag
     9361 atcattttcc gaaaataaaa aaggccaccg tagtcattga atacggtaag cctatttatc
     9421 cgaaggaact ggacaaggag actcgcaaga gcatgggcac ctatgtacag aatattatct
     9481 ccgaaactta tttcaaaaac aaagaactga tctgatcttt ctaatgtcta tccgcgaggg
     9541 agacctgctg ggctatcagt aacagataat ccgcagcgtc tccctcttcg ggataataac
     9601 tcaccgaatc ccggttccgt cccatcaggt aatgcagaaa atcctctaaa atctccgtgt
     9661 actgataact gtataacaca taattagcac aggacaattc ttctgcccgc ttcagaagat
     9721 cctcggttcc attgttgaca ttcattacgg catcgatcat gttatgggaa cgtttggcca
     9781 gctcctcgcc ccgattccgg atgctttcca tgaatacagt gcacaggtcc acatccaccg
     9841 gctgtctggt ggccagatac gtcatggagc catacagata ggccgtttct tccagataac
     9901 tggtattgtc ctcgaaaaag tccttatact ccaggatctg attgcggtaa gtgtatagtc
     9961 ccgccgcccg gtaaagctcc gtcagcgcca tgaatttctc cgcatctctg ccggatgtcg
    10021 acatcagctt ggtgtacacc gacgacgcat gctgcagaca ttgtgtggca tattgcacat
    10081 catatttctg gtacagataa ctgaattttg ccatcaccgc cacgtaacac atgttctccg
    10141 gttccggctt ttctgtgtcg ttggccgttt tttccagcca gtcggcaata gattccagca
    10201 gatccgggat atcattgcca ttatcatccg caagcacctg cggataccac tcacaggcct
    10261 ccagcaaagt gatgatctca tcctcggtaa tactgcgatc ctgacatctg tcatgaacat
    10321 ccgtgcacaa cgcttccaac agttcctgat agtggtcttc tttcagagaa aatgtcaggg
    10381 atcttcccac attgtcacac tccagataga actcaccttc cccggtatag tccgtgaaat
    10441 ccgctgttcc gatgtacaga gacagctccc cgttgtaatc tgtctgcttt accgtgcctt
    10501 catagaccgt ctctccggtc tcccggtcca ccagccggaa agtctctacc ggctgtctgc
    10561 ccttcaccac tgcctgtttt tcaccgaccg cggcataccc ctgctgatcc accagcacat
    10621 tgggtgtcag cgtaggcact gtatagttga tgaccggtgt gttttccatg ccggtcatac
    10681 ttatgcttac atgccctgtc tctgccgttg cctgcccgca ggcgttcata cctatactgt
    10741 taaatagtaa tagcagggac aggatccctg cacttttcct gctgtatctc aaaatattcc
    10801 tccggtcatt ccctggggac attacagtac acgatctccc cgttgataat agtataaagg
    10861 gttcgtgtga aaatttccat aggatttccg tcgaaaatgg caatgtctgc atctttcccc
    10921 acttccaggc tgccaatgcg gtctgccacg ccacagattt tcgcaggata aatggtgatg
    10981 gcccggcaac cttcctccat tggaagtccc gcttttaccg caagtccggc acacaacggc
    11041 agtgtctgga tctgtgacac cggatgatcc gtggtgatgg cgaccatcac accggcccgg
    11101 ttcagcactc cggcggtctt aaaagacatg ttctgcacct cgatcttgtt ccggctggta
    11161 agatccggtc ccacaatggc cggaaatcct tccttcgcca gttcttccgc caccagatga
    11221 ccttccgaac aatgatccat cgtcattttt ataccgaatt cttttgcaat gcggattgct
    11281 gtgaaaatat cgtccacccg gtggacatgg gcttttaacg ggatgtctcc ccgcagtacc
    11341 ggaagatagc actctttttc aaaatccggt gcaaaatact ctcccttctc cgccgcttcc
    11401 tgcttttgcc gcagatattc tctgctctcc cagagttccc tgcgcagcat ggcggcgata
    11461 gccatgcggg tgacaggact tttgttctgc ccggaataat tgaccttggg gttctctcca
    11521 aaggccacct tcatggccgc cggtgatttc aggatcaggt cgtcgatccg gcgcccctta
    11581 gtctttacca tggcaaactg gccgcccacc acattagagc ttccgggtcc gatcatggct
    11641 gcagtaattc cggcccgcac cgcatcgtca aaggctgcat ccatgggatt aataccatcg
    11701 atagcccgca gagccggtgt caggggatcc actgtctcat tacagtcatc cccttccgtg
    11761 ctcttttttt cttccatgat acccatatgg cagtgtgcct caatgatgcc cggcatgatc
    11821 agtgcatttt tcgcgatcac tacctgctct ccttcttccg gctgtaactt cacctcacct
    11881 ctggcaccta cctgtgcaat tttcccgttt cttatctgca ggatcccttc ggggatctct
    11941 ctgcccgcca tggtcaggat cctgcctcct acaatataca tcagcggaac aatgcctccc
    12001 cgttcaccgg ttccccgttg ttggtcagag caaagtatac gttgcttcct tcggtgctgt
    12061 agtatttcgt gggcgctgcc acactgccga tggtgtcgcc gggattcaca taggaattct
    12121 ctttcactgt gatctccgtc agctgtccat aagtggcctc gtagccgtca cccagatcca
    12181 gggtcaccgc cttgccgatc tcctcatttt cgaagattga agtcactttt ccctgtgcac
    12241 aggcggtcac tgccgtacct tcttccgcgg aaaaaatggt ggcgggattg tatttatact
    12301 gatccagtgt agcaaaataa gtgctcttgt ccatagagta atgcatcaga atatcactct
    12361 gcaccggttg ctgtaatccg tcgctttctg caaagtgcag tgtcttctca acgaccacat
    12421 ccccgcctgc tgtctgcttt cccccggtct cttccttttg tcggtcggca tcggaattct
    12481 gctgctcttt ctgtgcggcc tccagcaggg tctcctgatc cgtcagcccc ggaatctcta
    12541 cgttgccgga gcctacctcc tggggcatat agtccagatc atcctcggtg acaggagctc
    12601 ccgcgatctc atttcccgct gtattatttg ccgtcagtcc ggggatgttc tcgtcctcct
    12661 tcagaagatg ttcgtcgtta ttctccgtga tctcctgtaa tttgtcatcc gcattgttgg
    12721 gaagcgcagt gaagtccaag gtataaccat catccctgga ctccacattc tgccccttca
    12781 tatagacccc tgtcatggtc aatgctgcca gtacaaaggc tgaagaggct aacattataa
    12841 tacgttcttt gttaataccg ctctttttat ttcttctcat atcctatccg catccttcct
    12901 gttccctgag tcaactgcct gctatacaat cgtttcaacc actttccctg gtttatccta
    12961 tagtcttgcc cgattcatgg gttttattca gaatgtcttc gatatgaaat ttgcatttat
    13021 ttggaactat tcagagccat gtaaatagcg tcgaatgttt gcattcgtaa gaacatttga
    13081 tgctatttat agggcgaagt aactgaatag ttatatttat tctatgcctg tgcggcatag
    13141 gcagccatat ccacgtgctg taccgtaccc acatttccgt tttcgtataa gaaaataccg
    13201 gtcttgcgga ccacagcatt ggtcgcattg gcattggtag tgccgtcctg agccttacgg
    13261 tctccctgca gggtaaaatc cgtagaagcg ttttgcagac agatagcacc gacacccttg
    13321 tcagaaagtt tgtacagtac gtcgttgccg ttttcatcct tgcaccagat ttttaatttt
    13381 gaccagatgg aatcgttctc atcgatccag ccattgccat cctcatcata ctgcgccaga
    13441 tctgcaaagc catccccatt ccgggtgccg aacagctcac tgccgtcgtt gatagtgccg
    13501 tcaccgttct tgtccagtgc cagatagccg ctgccattca gcacggagat ctcttcttcc
    13561 ttgccatcgg catccagatc aaaataaaag gtctgatccg acacttctgc cacatcagta
    13621 tccaggttga tcaccagcgg atcatggagc gtgaattctg ctacattgag ttgctctttg
    13681 tagtattcct cgcatctgcg gctcatattt acatttacat taaaattgat ctctctgcca
    13741 tccttggtcc gtacgcttcc cacggtggaa aaactggtat cctcctgttc tatgttccag
    13801 gtctcgctca catagttcaa tattttcaca cgggacgtga ccagattgaa cacgtctgat
    13861 gcaggtgttg cggttccctg tgtgctgccg gaattcgtct gggtattatt tacgctattc
    13921 cctgtattcc agctgtttcc ggtctgatta ccgctgctcc atccgttttc ctgcatccag
    13981 tcccgcaaag tgctgttcct ggacgggaat aataaatcaa aaatataacg caccgtatat
    14041 tcccggatgg tagacaatgt ctgttcactg gcactgcttc gcatatccca tctggcagaa
    14101 gataccagca aacgactctg ccagtctttg gctgtcatcg tggtgttctc tgcagtgttc
    14161 tcctgttcct ccgtgttctg tgtggtatcc tcaccattcg gtgtcttctg tcccttagtg
    14221 gatacatctg tcagacctgc ctgataatcc gttatctcaa atcgtctgac gcttgtctta
    14281 gaagcgtgat agcttctggc ggaatccatt cctatgcttg cggaatcaat tctcaaatct
    14341 gtaattttct cctttccccg acctcaccac gaaccaacag ggcgcgcttc ctgtaacgct
    14401 taacagttca ccgccgcatg cattcataaa agcgcctgct ccgcagtcga cgccgctaag
    14461 cggctcatct tttatttcat acatgcggcg ctccgctcat gcaatatcat atgttctgct
    14521 ttgtgtgcca gcacccacgc agaacatatg atattgcatg gctgaactgt taaactaaaa
    14581 aaagatggta aagaaacaga atacatataa tatgcctctg tctggttacc atccgtggtg
    14641 tcattatcaa actgtaaaaa ataagtagca ggaagtgact ggccttgtca cctctctact
    14701 acttatatcg tccgctatgg acattttatt tagtgttaat ttactgctgt tggggctgag
    14761 tgggagcgcc aggggttccg ggtgcgggag gcaccggtct gccggctgct ttcagctttg
    14821 ctgcgatcat gctggctacc atttctctgg tcaccacgat gttgccaccg aagggattga
    14881 tcacgaatcc caacgccggg ctgatattgc cctgtgcatc tttgcgcagc agcattcccg
    14941 cataatcgtc aaagttcatg gcgaaagtct gctgattctc ctcatctctc caccttttta
    15001 gctctgtcca gtcagtgaac gccatgaaga acttccgacc gtcctcggtg gacagcatcg
    15061 ggaactgtac tttggcatct cttgcgatgg tcacccggcc gttttcatcg ggcacaggca
    15121 ccggatcgat cacgaccgga gacaggaact ttgctttctg cagttcatcc aggaacatct
    15181 gtctgtgttc cggggtgtcc tcagctttta ataattcaat ggatcccacc atcatggggt
    15241 tggataccgg tttgttaaat tccatatttt ttctccttac ttctcattgt aactattcag
    15301 cctctcttcg cgaaacgcat ctgcgatggt tagtcacatg tctctgaata gttacactcg
    15361 gttatagttg atcaggcatc ccttcaggat gatctgacgt tcttcgtcgg tcagctctcc
    15421 cagcttcagg gtgaattcct tcatgccttc tccggttgtt accacatatg ccttaatttc
    15481 ctcggccttg tcttccacgg ctttcttgat gccgggaacg aacagataat ccagattctt
    15541 aaaaggcagg tctccgggct ggatgaggaa gggaagcata ccccagttga tcaggttgga
    15601 acgataacgc ttggtagcat actcgttggc gatgttcgcc cagccgccta ataccttctg
    15661 gcaggaagca gcctgctcac gggcagaacc gtctccgggc ttcactgcga aaatagtgga
    15721 accgaatccg gtgttctcac gacttgcatc gggatactgt ttctttactt cacccataac
    15781 atccttcagt accggaactg cctgacaggg atgtccgccg ttcatacgct ctttctctgc
    15841 cttctggatc tccttggcac gtcctacata ctgcggatcc ttacggctta aagtgaactc
    15901 tgccagtccc agaggattgg agcggtagga agaggtctca ccggatggaa tcagctcatc
    15961 ggtagtagtt acgggatcgt ggatctcgga tactacctgc agtaccagat tctcaggcag
    16021 ggcacccata gcgggccagt ccttgatatt ggggccgaac tggatctcta cgctctcgtc
    16081 tgccacaccg tgggaatcga a
//
LOCUS       DAWDHD010000047        15946 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_249_length_15946_cov_8.712730, whole genome shotgun sequence.
ACCESSION   DAWDHD010000047 DAWDHD010000000
VERSION     DAWDHD010000047.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 15946)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 15946)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15946
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_249_length_15946_cov_8.712730"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         4505 a         3241 c         4461 g         3739 t
ORIGIN      
        1 cataaagacg gacggttacg gacacggcgg tgttcccatt gccagaatgc tggaggatgt
       61 ggattttatg ttcggctatg ccgcagctac ctacgaggag gcacatgtcc tcagggaagc
      121 cggtgtgaag aaaccgatcc tcatcctggg atatactttt ccttattgtt atgaggaaat
      181 gatccgggaa gagatccgcc cggcagtgta tcgcagggat accgtggaag aactggcaga
      241 ggccgcagca aaggccggta agaaagccaa agtgcacatc aaagtggata ccggcatggg
      301 aaggatcggt atcactccgg atgacgaagg gctggatttt gtaaaatttg tcatggaaca
      361 tcccggactg gaagtggaag gtatctttac ccattttgcg aaatcggatg aagcggataa
      421 gacttctgcc aaccatcagc tggaactgtt tcagaatttt attgatagaa tacagtcgga
      481 actgggactg gagattccgg taaagcattg ttccaacagt gcggcaattc tggaaatgcc
      541 ccaggccaac atggatatgg tacgggcggg aatcacgacc tacggtttat atccttccga
      601 agaagtaagt aaggatattg tacctctgcg ggctgcaatg tctctctaca gtcatatcat
      661 ttactgcaag acgatccatg cggggcagag tgtcagctac ggtggattgt ttaccgcaac
      721 aaaagatacc agagtagcca ccatccctgt aggatatgga gacgggtatc ccagaagcct
      781 gtcgggaaaa ggctacgtgc tgatccacgg gaaaaaggcc cccatcctcg gaagggtctg
      841 catggaccag ttcatggtgg atatttccgg aattcccgga gccatggagg gcgataaggt
      901 gacgctcctt ggcgcggatg gtcaggagcg gatcacggcg gaagaactgg gagaactttc
      961 cggtagattc aattatgagt ttgtctgcac actgggaaaa cgaattccca gagtctaccg
     1021 tcgaaatggt gagatcacag aagtaaaaga ttattttgaa aatttctgac ggatctaaag
     1081 tgttcgtata actccggaag aatcggcaat acttatgtta aatcgctgat gaaggagtag
     1141 aaatgagaag aggagatatc tattacgcag atttgagacc ggtcatcggc tcggagcagg
     1201 gcggcatccg gccggtgctg atcatccaga acgatgtggg gaacaaacac agtcccaccg
     1261 tgatctgcgc tgcaatcacc tcaaagatga acaaggcgaa gctgcctacg catattgaac
     1321 tgaatgcccg ccgctacgat atggataaag attccgtagt gctgctggaa cagctgcgca
     1381 ccatcgataa aaagaggctg aaagataagg tatgccatct ggacggagac attatgcgga
     1441 gagtgaaccg ggcactgaaa gtcagcctgg agctggatac atagcccgat gcatagtgta
     1501 tccgaaaata ggataaaata cgaaaggatg aaataaaata tggacatgag tgggcctgtg
     1561 gccatttttc tctttttcct gctgttgctg gtggatatgt ttttctatgg atttgatgcc
     1621 gccatcgaca atctgaatga aaaggagatc ctacataaag cagaggaaga aaaggatcgc
     1681 cggtccatgc ggctgagtga aatgatcagc cgtccgggga tctatatcaa tacggtacag
     1741 ctggttatta cgctgatcaa tattttaatg ggagcttttt atctgaatat ctggctgcag
     1801 accattgaca agggacttca tgtgatcaat gtgggaggca tgtctctgga gaatgtgcct
     1861 acgggagtgc tttcagggat tgccacggta ttgtctttta ttgccatgat ctatatcctg
     1921 ttgactttcg gtgtgctgat tccccggaaa atggcatcca gacagccgga gaagtgggcg
     1981 tatgcctgta ttgacccgat ttatattatc accagactgc tggcacccct gacggggctg
     2041 gtgaatctga cagccaaggg aatcctgttc ctcttcggtg tccgcaacaa tacggatgaa
     2101 aatgatgtga ccgaggaaga gatcatcaat atggtgcagg aaggtcatga gcagggtgtc
     2161 attcaggcca gtgaggcgga gatgatctcc aacattttcg aatacggaga caaggaagcc
     2221 caggatatca tgacacatcg tggaagtatc gtggccattg atggtgagac gaaactgaaa
     2281 gatgccattg cttccatgct gggaggcaag aacagccgtt accctgtgta tgaggagaat
     2341 attgatcata tcatcggtat cctgcatctg aaggatgcca tgcggttcca tatcagtgat
     2401 gacaagctgg acctgcctat cgcaaaactg gacgggctgc tcagagagcc ttattttgtg
     2461 ccgcagacga aaaatatcga tgaactgttc aaggaaatgc agtcccagaa gctgcagatg
     2521 gccattgtgg tagacgaata cggacagacc gacggactga tcgccatgga ggatattctg
     2581 gaggagatcg tcggcaatat catggatgag tatgatgagg atcaggaata catcaagaac
     2641 cgtggcaagg gcgaatatgt catggaaggc aagactccgt taaaggaagt ggaagagctt
     2701 ctgggtatta ccttcgacga ggaagaattc gagactttga acggtttcct gatctccaga
     2761 ctggaccgga tcccggaacc ggacgagcag ttcgatgtgg actataaggg atacaacttt
     2821 aaaatccttt ccgtggtcaa aaaaatgata ggaagtgtgt tggtccggaa gcttccggag
     2881 gaaaaaacac cgcagaatgc agaagacgga caatcagtgc ttgaagaaaa aagaaaataa
     2941 tgctatgata aaaagagcgt tattgcgtaa agactacgca tacaaagaga aagaaacttt
     3001 aagttcaaag gagtaacaaa aatgtcagga cattccaaat ttgctaatat taagcataaa
     3061 aaagaaaaga acgatgctgc aaagggtaag atttttacca ttatcggccg tgagatcgca
     3121 gtcgctgtaa aagagggtgg tcccgatccc gccaacaatt tcaaactggc aaccgttatt
     3181 gccaaggcaa aagccaacaa catgcccaac gacaccatcg agcgcggtat caagaaggca
     3241 gccggagatg tgggcaatgt aaactataag tatgtgactt acgaaggtta cggccccaac
     3301 ggtatcgcca tcatcgtaga tgcacttacc gacaacacca accgtaccgc tgccaacgta
     3361 agaagcgcat tcaccaaggg acagggtaat gtaggtactc ccggatgtgt atcctttatg
     3421 ttcgataaga agggacagat catcgtggac aaggaagaat gtgatatgga agcggacgat
     3481 ctgatgatga tcgctctgga tgccggagcg gaagatttct ccgaagagga tgacagcttc
     3541 gagatcctga ctgatcccga taacttcgaa gaggtacgca aggctctgga agatgccgga
     3601 attcccatga tcagtgcaga agttaccatg attccccaga attatgtaac actgaccgat
     3661 gagactgcag tgaagaactt acagagaacc ctggaactgc tggaagatga tgatgacgtt
     3721 caggcagtat atcataactg ggacgaataa attgaaaggt taggggtaac agcatggaga
     3781 aacttgcaat tgtagttcct tgttataatg aagaagaagt gttaaagatt gcttccgagg
     3841 cactgagggg tgtgctggat gatctgatcc ataagggaaa gatcgcggaa gacagtttta
     3901 ttctgtttgt aaatgatggc agtaaggatc gtacctggga gctgatcgaa gaagagcatc
     3961 aggcacatcc ggtgcaggta tgcggtgtaa agctggccgg caatgtggga catcagttcg
     4021 cactgactgc cggactaatc actgccatgg atctgtctga tgtgacggtt tccattgatg
     4081 cagatctgca ggatgatgta gctgtgatcg aggaaatgat cgataagttc cacaacggct
     4141 gtgatatcgt atacggtgtc cgtaaggagc gtaagaccga tactttcttc aaacgtacca
     4201 cggcgcaggg attttataaa gtcatggaaa tgatgggggt caagactgtt tacaatcatg
     4261 cggatttccg actgatgtcc aagcgcgcgg tagaacagtt ctccaagtat caggagacca
     4321 atctgttctt aagaggtatg atgcctttga tcggttatca gacggactgt gtatactatg
     4381 accgtaagga aagagtggcc ggtgagtcca agtatcctct gaagaagatg cttgcattgg
     4441 cattcaacgg tatttcttcc ttcagtgtga agcctatcag tatgatcctg ggtgccggta
     4501 tgatcattgt ggccttaagc cttctggcag ctatttatgc gctgatctct tattgtaccg
     4561 gacatgtggt accgggctgg acttccctga tcctgtccat ctggttcctg ggtggtattc
     4621 agttaatggc catcggactg gtgggccagt atatcggcaa gatttacatt gaggtaaaac
     4681 atcgtcccag atataacatc gaaaaggtac tgaaataatg aattataaaa agaatgtaat
     4741 aagtgtaata ctgtggttac tttctgcaat tatggcggga accggagcga tgatggtcac
     4801 tatgccaagc gctccggagg aatcccaatg gatcggcctt gtgatagcag gtgtatggct
     4861 tgctgtcaca gggcttgttg cttttctgct gtcaagagga ctgcaaaaga gcagagagaa
     4921 aaaaacaaac gtagataatc ggcagactgc acttgtggtc gaaggaatcc tcacagtgct
     4981 gttggtagcg gcggggattg tcctgaggat tcgtgaaatc cagctgatgg atctggatgt
     5041 agcggcggag gatgtctggt ttgatacggt ccgagtgaca acacagacgc agatcccaca
     5101 ggtagtacat ggcgcggttt atctgtatct gcagatactg cacgggcttt tgctgctcct
     5161 cggcaataag atcaccgtgg cactcggatt ccaggcgggc ttacagattc ttggctggat
     5221 tctttgttat tttgcggtcc gccgattaac tggaccggct gcggcagtta cggcgctggg
     5281 atttggaatg ctctggccat tgtgctggaa ggtagtagta ttgggaccgg aggaacttta
     5341 tcagatgtta tggatgatag gcctgtgtct ggttgcggca gcactgaata ccttccgacg
     5401 aaaaggtgaa cagaagggcg tccgcccgat tctcagtttt tttatcgctg gtgtggcaat
     5461 gggagtgctg acctatctgg atatcacggg tttattactg tatcttccga tactttcggt
     5521 ttttgcactg gagacagcgg aagaggaaac tgtgggattt ttacatagag tgggggccgc
     5581 tttacgggct gcgttcgcag gcgctgtcgg aggctttgta tcaatcgcag ttgacgcctg
     5641 agtcagcggg aaacttatgg gaaatgttct cactgcatgg ggacggctgt tccaaccgtc
     5701 tgctttttcc tggccggttg caatttacga cggtactatg acgaccggag cggatactgt
     5761 ggtagccggc aagattctga tctttttatt ggtaacaatg caaattctcg gtgtatttgg
     5821 tttctggtgt cagaaacgca gagaacgtca gagtatctgg atccttttgt ggattgtgtt
     5881 ggagttactg atgacgtttc agatcacaac cccggatatt cagggaatgt ggatgcatgt
     5941 ccggcttcgt tatcttctca ttggagccgg agtacaaagc ctgattccct atgtgaccat
     6001 gatgcaggaa aaagaaaggg agagtacgga aacggtgaaa cgcagatccc ggctgaaagt
     6061 acaggatctg gagaccgagg aattcccgga ggaagagcca gcggatttcc aggaagaacc
     6121 tgtagaaacg gaaaaaatac agctgatcga aaatccactg cccctgccga aaaagcatgt
     6181 gcctaaggta ctggattata agctgaacgg tgatgatgac agtgattttg attatccggt
     6241 agcggatgac gatgattttg atcattaaaa atctgttggt ttcctaatga aatgttggat
     6301 tgacggacat actaaaagac gatacgagag gtggaatgcc tttgtgtcgt ctttttttgt
     6361 tatttaaatt ttcaaaatca taaaatcatt gaaactggga ggaaacaggc catgcagaat
     6421 cagaaagata acgatcagaa caagaatgaa gcgccgaaga ccgaggcgga agaacacaga
     6481 gacgaaagaa ttgaaaaaac gggacaactg acgctgtcgg atgcggcatc cggggaaaaa
     6541 atccatctgc tgaccattat cggtgaaatc gaagggcatg ataatctctc cggtaattcg
     6601 aagactacga aatatgagca tatcctgcca caactggcag ccatcgaaga cagtgaagaa
     6661 attggcggtg tgctggtact tttaaatacc atgggcggag atgtggaggc gggactggct
     6721 attgcagaga tgctggcatc tttaagcaaa cccaccgtat ccctggtgct gggtggcagt
     6781 cattccatcg gggtgcctat tgcggtgagt tgtgactatt ccttcatcgt tcccactgga
     6841 accatggtga tacatccggt aagaatgaat ggcatggtga tcggtgtgcc ccagaccttt
     6901 gaatatttta aactgatcca ggaccggatc acgggctttg tatgcaaaca ctgtaaaatc
     6961 agccggacaa aactggaaga actcatgatg gagaccggat tcttaacgaa ggatgtgggg
     7021 agcattctgg tgggagaaga agcggtgaac cacggtatta ttgacgaagt gggaggtatc
     7081 gaccgggcca tctgcaggct tcggaagatg atcgaaggga acagaaaaag ggatgacaag
     7141 gccatggaaa aatgatatac ttctaattat gactataatg aagttagggg tatgatgaca
     7201 tggcatcttc atcaggcaaa agaacatcat cttccaatag gacgagaaaa agtaccacaa
     7261 acagccgcag caggcaggcg cagagaacag cacaggacag tgaactgttc catgagatcg
     7321 gtctgatcgt tttgtttgtg gtaatggtga tattattttg ttgtaatttt ggtattatcg
     7381 gcccggtcgg caatgcgatc agcggggtct tattcggtat ctttggattt actgcatata
     7441 tagcacctat tgtgatattt ctggctgtgg ctttctggtt cgccaatgag gggaacccta
     7501 ccgctgtacg caaaatgatt gcaggtgtcg tattgtttct gatgctcggt gtgatctgtg
     7561 atctgatcgc caggactgcc ggctctatgg aacagtatga tatcaaactg ttatatacaa
     7621 actgcagtac cggtaaaaag ggcggaggta ttctggcagg aagtatctgc tatctgttgc
     7681 tgcattatct ggagacggta ggaacggttc tggtggtctt attgtgttct gtgatcagtc
     7741 tgatcctggt aacggagcgt tctttcttaa acagcatgcg aaacggtggt gaccggatcc
     7801 gggaactgtc tagagaggat gcagtgcgca gacgggaaaa tgcacagatc cgcagacagg
     7861 aacaggagga acgtaattct cagagagagg aagcccgtcg tatccgggaa gaggaacgac
     7921 ggatccgtga ggaagagcgc agactccgtg cggaagagaa agagaatgag cagatcttaa
     7981 acgccattcc ccgcagagag aaaaaagtca gcggtgttat gatggacact tccctgaaaa
     8041 agcaggaggc acagcctgtg ggcagacggg aagatattca tgagatcgtt tatgaagagg
     8101 atgcacagga gattcctgtt gtggaagaga ccagagtgac ctccagcgat tttgataaga
     8161 tcaaggtaac gggaatgcat caggtgacca tgcaggagac cgcagcagaa gaaccttacg
     8221 aggagatccc cgtggaggaa gtgaccagtg ctgctacctt caaaaaacct tttgaacgca
     8281 gagaatcctg gcaggaggag gcagcactcc tgacacctca ggcggaatct atccggattc
     8341 acaaggaaga tatcccggag cctgtagagg aaccggttac ggaatggcag tcatcctccg
     8401 atatgcggca gctgcagccg gagactgagc ctgtcgtgcg ggaacctgca gagacagcaa
     8461 cggcggaaag ccgcatacga ccgaaaatca cggcaccgga gccgccaaag ggtaattctc
     8521 tggcagagga tcagatacat aaggatattg cgaaagcggc caaacagcct ccggcagaat
     8581 accggattcc acctctgagc ctgttacaga agggaaaagc agctaccgga gattcctcca
     8641 gagaattgaa agagaccgcc atgcgcttgc agcagacgtt aaatactttc ggtgtaaagg
     8701 taacgattac cgatatcagc cagggtccca gtgttacccg ctatgaacta cagccggagc
     8761 agggcgtaaa ggtatccaag atcgtaggcc tggcagatga tatcaagctg aatctggcgg
     8821 caacggatat tcgtattgag gcaccgatcc ccggaaaggc agctatcggt atcgaagtcc
     8881 cgaacaaaga gaacatgaca gtagcactcc gggatctgtt agagagcaaa gagtttcagg
     8941 agtttaattc caatattgca tttgccgtag gtaaggatat cgcgggcaag actgtggtgg
     9001 cggatattgc caagatgcct catatgctga tcgccggagc taccggttcc ggtaagtccg
     9061 tatgcattaa tacgctgatc atgagtattt tgtacaaggc gcatccggat gatgtgaaac
     9121 tgatcatggt ggatccgaag gtcgtggaac tgagtgtcta taacggcatt cctcatctgc
     9181 tgatcccggt agtgacagat cccaagaaag catctgctgc attgcactgg ggcgtcagtg
     9241 agatggaaga ccgttatcgg aagtttgccg attataatgt ccgtgatctg aaggggtata
     9301 acaagaaaat agagactatg cctgttccgg aaggagagga agcaccgaag aagatgccgc
     9361 agatcgtcat tatcgtggac gagctggcgg atctgatgat ggtatgccca ggcgaagtgg
     9421 aggaatctat ctgtcgtctg gcacagttgg ccagagccgc aggtatccat ctgatcatcg
     9481 ctacccagcg acccagtgtg gatgtcatca ccggactgat caaggccaat atgcccagtc
     9541 gtgtggcatt ctccgtatcc agcggtgtgg attcacgaac gatcctggat atgaacggtg
     9601 cggagaaatt gcttggtaaa ggcgatatgt tattctaccc gcagggttat tccaaaccgg
     9661 ccagagtaca gggtgctttt gtgtccgaca aggaagtatc cgatgtggtg gattatctca
     9721 agaaccaggc actgggcaat acctatagca actatgcaga agatattgaa gagaagatca
     9781 aaaatatcgg ctcgtcaggt ggttcctccg gaagcggttc tggtggcggt aacgacagag
     9841 acgaatattt tgaggaggcc gcaaggttta ttattgataa agacaaggcc agcatcggca
     9901 tgttgcagcg tgtgttgaaa atcggtttca accgtgctgc cagaatcatg gatcagcttt
     9961 gcgaatacgg cgtggtaggt gaagaagaag gcactaaacc cagaaaagta cttatgagca
    10021 tggaacagtt cgaacagctg ttggaggaaa tctgacattt gttcgagact gtctgaataa
    10081 acggagggaa aaaggacatg aaaacagata ttgaaatcgc acaggaagcg gtaatggagc
    10141 ccatcaccaa agtggctgaa agtattggca ttgaagcaga tgacctggaa ctttacggaa
    10201 aatacaaggc aaagctttcc gaagaatttt tacgtaagat ccaggatcgt cccaatggaa
    10261 aactgattct ggtaacagct atcaacccaa ctcctgcagg agagggtaag accacgacca
    10321 cggtaggtat gggagaggct ttcggcagaa tgggcaaaaa ggctgtaatt gcgctcagag
    10381 agccgtcctt aggcccctgc tttggtatca aaggtggagc tgccggcggc ggttatgcac
    10441 aggtagtgcc tatggatgaa ctgaatctgc attttaccgg tgattttcat gccatcacct
    10501 ctgccaacaa cctgttggct gcgttgctgg acaatcatat ccagcagggc aatgatctgg
    10561 gcatcgatac ccggcagatc gtgtggaagc gctgtgagga catgaatgac agagtccttc
    10621 gtaatattgt ggtaggactt ggcaacaaga cagacggctt tgtcagagag gatcattttg
    10681 tcattaccgt agccagtgag atcatggcga tcctgtgtct ggcagacgat atgaaggatc
    10741 tgaaggagcg cctgtccaga atcgttgtgg cttacaacta tcagggtgag ccggtgaccg
    10801 ccggccagct gaaagcagtc ggtgccatgg cagcactgtt aaaggatgcc atcaagccca
    10861 atctgatcca gaccttagag catactccag cattggtaca cggaggtccg tttgccaaca
    10921 tcgctcacgg atgtaactcc gtcagagcta ctaaggcagc catgaagatg gcagactatt
    10981 gcatcacgga agccggcttc ggtgcggatc tgggagcaga gaaatttttc gatatcaaat
    11041 gtcgtaaggc aggacttact cctgatgcag tggtactggt agcaaccatc cgtgcactga
    11101 aatacaacgg tggcgttgca aaggcggatc tgggagcaga gaatctggaa gcgttgaaaa
    11161 aaggtattgt gaatctggag aaacatattg agaatcttca gaaatatggt gtgcctgtag
    11221 tggttaccct gaactctttt atcaccgaca gtgaggcgga gaccgcttat gtcagagaat
    11281 tctgtgagac aagaggctgt gagttcgcac tctccgaagt gtgggagaaa ggcggagagg
    11341 gtggaatcgc tctggctgag aaagtattaa aagcaatcga cgaaaaagag aaccacttcc
    11401 atgttttata tgaaaatgaa ctgtctcttc aggataagat caaaaagatc gcgcaggaga
    11461 tctacggagc gtctgatgta gtgtttgctc cggcggcagc aaaacagtta aagagactga
    11521 cggaacttgg ctttggtgat ctgccggtat gtatggcaaa gaaccagtat tctctgtcag
    11581 atgatcctac gttactggga agaccttccg gttttgaatt gaatgtaaga gaggtctatg
    11641 tgtctgcagg tgccggtttt gttgtagtgc tcaccggtgc ggtcatgacc atgcccggcc
    11701 tgcccaaggc tccagctgct tacaatatcg atgtggatga cagcggcagg atcgtaggct
    11761 tgttctagta aggaggaaag tgtctcatgg cacagttaat agatggaaaa gttatttccg
    11821 cacagattaa agatgaagtc aaagaaaaag tcgcagggtt aaaagagcag ggctgcgaag
    11881 taacgctggc ggtgatccag gtaggtgcgg atcccgcatc ttccgtatat gtccgcaaca
    11941 agaaaaaggc ctgcgaatat gtaggtatcc ggtctctggc atatgaactt ccagaggaga
    12001 caacagagga aaaattactg gagctgatcc gggaattaaa tgacagaacc gatgtcaacg
    12061 gcattctggt gcagctgccg ttaccgaaac atattgacga agaaaaggta ctggattcca
    12121 tttctccttt aaaggatgta gacggatttc atccccagaa tgtcggtgca ctctgcatcg
    12181 gcaagccggg ctttgtgtcc tgtactcctg caggagtcat acagctgctg aagcgttccg
    12241 gtattgagat cgccggaaaa gagtgtgtgg tggtaggcag aagtaatatc gtcggcaagc
    12301 ccatggcatt gttgctgctt cgggagaacg gtacggtgac ggtggctcac agccgtacca
    12361 aagatctgaa agaagtaacc aaaagagcgg atattctggt ggtggctgtc ggcaagccga
    12421 agatgatcac ggcagaatat gtaaaagagg gtgcggttgt cattgacgtg ggaattcacc
    12481 gcaacgaaga aaacaagttg tgcggtgatg tggactttga cagtgtggag cctgtatgca
    12541 gcgccattac tccggttccc ggcggcgtag gtcctatgac catagctatg ctgatgaata
    12601 actgcctgga atcggtatcc ttgcaggcag taagaggata attatatgaa gtgtccgaat
    12661 tgcggggcgg aaatgaaaga aggtacgctt tactgcgagc actgtgggga ggatatccat
    12721 attgttcccg attttgaacc ggaaattgaa tacaatatgg agcagactct gcatgatatt
    12781 gccagggaaa tgacaccgga agaagatgcc acggacgatc aggatgcaca gcagagcatg
    12841 acggaattgc aggactggga tgaagaaccg ctggaaaata cggcaaaaca tccctttcgc
    12901 tggaaattca ttgtgctgat cctggtgctt gcggtagtag cggtggtatg taccggtttc
    12961 ggaatctctt attcccacta tcattccgct gaatatcaga taaagagtgc gagagagtat
    13021 gtagcttccg gggaatacga tcaggcggtc acctgttatg aacgtgcgtt ggaaatcgat
    13081 ccggggaaca tcaccttaaa atttgaactg gcggatattt attttcagaa gaacaacaaa
    13141 atggaatatg aatatctgct tcgggagatt gttgcggatg ccgcagctac ttccgaacaa
    13201 ctggaaagtg cttatggaaa gctgatcgcc atctatgcgg ccagggaaga ttataagacc
    13261 attaatgatc tgttgttgaa ctgtggtaat gtaaatatta tgagtaaata ccagagctat
    13321 atggcaatgg aaccggaatt cagtctgcag gagggatatt ataccagcat ccagcctttg
    13381 aaactgacaa ctttcggaag tggtaagatc tactatacta ccgacgaaac cgaaccggga
    13441 gaacacagtc tgctgtatac ggcacccatc cttttagaga gcggtgatta ctgtatcaaa
    13501 gccgtctaca tcaatgagaa tgggatcagt agtccagtgg ttaccaaaaa ttaccatatt
    13561 gagatcgagg aactgagtgc tccggaggta acggcggtca gtggggatta tgaattccca
    13621 atcaatatcg aggtgaccga cggatatgat gtttattaca caacggatgg ttccgatcct
    13681 acgcagaatt ccacggtgta taatggaccg atccccatgc ctcttggcaa atcagtcttc
    13741 cggtttatcc gcattgcgga aggaagaaag agcgagatcg tggagaaaag ctacaatttg
    13801 gtcatgaaca cggagtttat gccggaggat gccgtgacaa aagtagtgaa ttatgccata
    13861 gaaagtggta aaataacgga agcatcagga agctttgatg aatccgctgc cgcatatctc
    13921 tatcagtatc agtatgtgac gaacatcaac aagatagatg atttctatgt tgttgccgag
    13981 atctaccggg gagaagatgg gacagtcaca aagacgggga atctgttcgc cgtaaatgcc
    14041 tataccaggg aactgtacaa attgcagacc gacgaaagcg gccgccatta tacgcttggt
    14101 gatcgtttag atatataatc ctaacagaag ggacggaaaa agagaaatgt ataagatcgt
    14161 aaaaaaacag gagctgacaa ccaacatcta tcttatggag gtagaagcaa acagagtggc
    14221 aaagacctgt gaacctggtc agtttgtcat cgtaagaatg gatagcgaga gtgagcgtat
    14281 ccctctgact atctgtgatt acaacagaga aaaaggaaca gtaaccattg tgttccagac
    14341 cgttggtgcc ggtacaaaac tgatggcaca gctttccgag ggagaatact tccatgattt
    14401 cgtaggtcct ctgggatgtc cttccgaact ggtagaacag cctctggatg aggtaaaggc
    14461 aaagaagatc ctcttcgtgg ctggtggtgt cggtgcggca ccggtatatc cccaggtaaa
    14521 atggctgcat gagcatggcg ttgctgcaga tgttatcgtg ggaagcaaga ccaaagatct
    14581 tctgatcctg gagaaggaga tggaagctgt cgcaggaaat ctgtatgtaa ccaccgatga
    14641 cggctcctat ggaagaagcg gtatggtaac ccagaccatc aaggatctgg tggcagaagg
    14701 taaaaagtac gatctgtgta ttgccatcgg tcctatgatc atgatgaaat tcgtgtgtct
    14761 cttaaccaaa gaactggaga ttcccacagt tgtcagtctg aatcctatta tggtagacgg
    14821 tacgggaatg tgcggtgcat gccgtgtaac tgtcggcggc aaggtaaagt tcgcctgtgt
    14881 agacggaccg gagttcgatg gacatgaagt aaactttgat gaggcaatga gacgtcagca
    14941 gatctacaag actgcagaag gacgtgcaat gctggccctg gaagagggag atacccatca
    15001 cggtggctgc ggcaactgcc agtagtcata caaaagtttg aacacgataa atcagaaagg
    15061 catggttaaa ataaaatgga tgtattaaag agaattcctg tcagagagca ggatgcgaag
    15121 gaacgtgcta ccaatttcaa agaggtttgt ctcggatata acaaagaaga agcaatggaa
    15181 gaggcttccc gctgcattaa ctgtaaaaat gcacagtgtg taaagggctg ccccgtagca
    15241 atccagattc cacagttcat tgaacaggtg aagaacggca acattgaaga ggcttatcgt
    15301 attatcagtg aatccagcgc ccttcccgct gtctgcggcc gtgtatgccc tcaggaaagc
    15361 cagtgtgaag gaaaatgtat ccgtggcttc aagggagatc cgatctccat cggtaagctg
    15421 gagcgttttg tggctgactg ggcaagagag aatggcatca agcccactcc cgcagcacag
    15481 aagaacggta agaaggtagc tgtgatcggt tccggccctg ctggtcttac ctgtgccgga
    15541 gatctggcaa agctgggata tgatgtgacc attttcgaag cactgcatga ggcaggcggt
    15601 gtactggtat acggtattcc cgagttccgt cttcccaaga gcggcgttgt ggcaaaagag
    15661 atcgagaatg ttaaatctct cggtgttcat attgaaacca acgtggtggt aggtaaatcc
    15721 gtgaccatcg atcagctgat ggatgaagag ggcttccatg ccgtatttat cggttccggt
    15781 gctggacttc cgaagttcct gggtatcccc ggtgagaatg ccaacggcgt aatctctgcc
    15841 aatgagtatc tgaccagaag caatctgatg aaggcgtttg atcccgattc cagaacacct
    15901 atcatgcacg gtaaaaaggt agctgtggta ggcggcggta acgtgg
//
LOCUS       DAWDHD010000048        15873 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_251_length_15873_cov_14.312239, whole genome shotgun sequence.
ACCESSION   DAWDHD010000048 DAWDHD010000000
VERSION     DAWDHD010000048.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 15873)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 15873)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15873
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_251_length_15873_cov_14.312239"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         4339 a         3286 c         4333 g         3915 t
ORIGIN      
        1 attatctgca gggatattat tttggaagac catgtggaaa gaaacagttt ctgcaacaat
       61 atgccagagg catcaacgtt aaataaagag agtaaggcag actcagctat gtggggctgc
      121 cttattttat ggtcttatca gaaggatgag ttcctaacgg ctagtctgag tctctccagg
      181 tcatctgcat cagggtgaga caatgcacag tcaaaattct ggatcaatac atcaagattt
      241 gccggatgat caggagcttc cttcattttc aagtagcgtt cccggacaga ctgcggcatt
      301 ctgccctgac acatatactc tccgaagata acattgcttg catcaacgaa ctgcttaacc
      361 tgattcagga ttttccggaa atagatatca ctgccaccaa agcctgcagt tccaaacaga
      421 aaaatctgtt tattctttaa ctgcgataat agctgtaagg tcttggtatc tgcatttccc
      481 ttatcggtcc aaaatccgat atagagcagt tcggaggaag ggatcacggt gtcggacaca
      541 ccgaaatact cacattcatc ctgaggtaat gcctcgtgga tcgcatcagc cagcaccttg
      601 gtattacctg ttaagctgct ataaatgatg gaatattttt tattcttcat atcatgttgt
      661 ctcctttcat aggatcatga aattatcata tcgcattcct attttagttc ggaaagcatg
      721 atttgtcgaa tgaattttcc ataaacattt tacgggggct gccccggtat catttaatga
      781 ggtagaaaag ttgacagaag agaaatatac gatccgaaag tataaaagaa ttttatttta
      841 tgtagtgata tgtgtgctgc ttgcggcatt ccttttggga caatatattt acccaagtga
      901 aagggaaccg gtcacggagg aaagcattac ttataccgga accttttacc gggtgctggc
      961 agacggaacc aaagaggaaa tcccggttcc gggcaaatgt gatgtgcctg ccggagagcc
     1021 tctggtcatc cggtcagtgc tcccacagga ttatcatgaa aatacgatcg ccatcaggtc
     1081 ctctctggag aatgtgcgca tttacatcgg tggggaactg agggcggtgt atgatacgga
     1141 gaataccaga ccttttggga aaaattctgc cagtgggtat gtgttctgcg agacatccgg
     1201 cgaggatgcg ggacaggaag tgcggattga actgcagtcc tttaccgaca agtattccgg
     1261 tgtcgtcaac acggtatatt gtggggataa atcagatatc tgggcatata tgttccactg
     1321 ctattttatg gtaactctga tcgcctgcgc catgctgttt gccggactgg tcgtgctgat
     1381 catcagtctg gtgcttgata ttatatataa gacgagattt gatctggagt acctgggatg
     1441 gtgcatgctc ctgggagcag tgtggatgct gggagaatcg aagctgcgcc agctgtttgt
     1501 ttccaatgct tctattctgt cgaatatgtg cttcttcgtt gtgatgctct gcccggttcc
     1561 gatcatgttc tatattgaca gtgtgcagca gggaaggtac cggaaggtct atcatgtggc
     1621 agagtgtatc acctgtgtga attttgtact atgcacggcc ttacagctac tgaatatcgc
     1681 agattttatc tcgacaatgt tcttgtccca cctggtgatc gcaggaacgt tcctgacgat
     1741 atttatcacc atttgccggg atctcatcaa gggaacggcg aaacattaca agcttccgct
     1801 gatcggactg gtagctgcca tgatcgcagt catgctggaa gtgacagcgg tgtatcgagt
     1861 ggtgtcattg tccgggatct ttatcgcaac cggactggtg gtgctgctgg tcgttacgct
     1921 gatccagaca atggatcgca tccgcgagct ggagctggcc aggcagagag aggccaggga
     1981 aagcctggat tatctgactg gtcttcccat gcgtcataag ggtgaaaagc tgatccttga
     2041 gaaaatgcag gagcatgatg gctgtctggg atttgtggat atggataatc tgaagaagat
     2101 caacgatgtc tatggacata aggcagggga ccgtgctctc aggctggtag gagcaatgct
     2161 cacagagtgc atggagaatg cagtggtctg ccggctggga ggagatgaat tcctgttcta
     2221 tctgccggcg gcatcagaag cagagatgag catgcggata agaaagctgt ttgacagctt
     2281 tggtgcggca aaaaatgtca cggcggagac ttcaccggca tccttgtcct gcggtctgtg
     2341 catgtgcaga cagggaggaa actttgaaga atattatata aaagcggata aggcgttgta
     2401 ttatgtcaaa cagaacggca agaataatta ccggttctat gaggagctgg aggaacagca
     2461 gctcacgaag cgatcagtaa ataatgaggg gatacttata aaagattctt agggtagtag
     2521 gagctgatat ggcgtatcaa tatgagaagg taaatcacga actgctggtt ccacagatcg
     2581 aagaccagct gatgatgtat atgaagcagg agaacatagc catcggagaa aagctgccca
     2641 gtgagtacaa gctggcggag ctgttcggtg tggggcgcag tacgatccgt gaggctgtga
     2701 agagtctggt gaccaagggt gtcctggagg tgaagagagg ctccggcacc tatgtcagaa
     2761 gcacggagat ggtggcggag gatcccttgg ggctggcaca gttcgaggat aagtatcgtc
     2821 tggctatgga actgttcgag gtcaggatcc tgctggagcc ggaggtcgcc gctgtggcaa
     2881 gcaggaatgc cacggaagag gagaagcagc atatcaaaga gctgtgtgat gagaccgagc
     2941 ggatctatct ggaggggaag aatcacatcc ccaaggatat ggaatttcat gaggctattg
     3001 caaaatgcag cggcaaccgc gtgtttgagg tacttgtacc cattatatac actgctgtga
     3061 cgacctttgc caatctgacc aacagacagc tcatgaagga gaccatagag acacacagag
     3121 ccatcaccga tgccatcctc cggggagaca gcatgggggc aaagtgcgcc atgatcatgc
     3181 atctgaccta caatcgtcag aagctcctgg aactgatcga acaaagagaa aagtcatcag
     3241 acaagttaag accataaaat gacaagcatt gataaattta tacgaaaaac cagcctgggt
     3301 aacaccatcg ggctgttttt tttatgcgat atgtggaaaa aagatgtaga tttgaaacaa
     3361 cttgaagaag gcggagaaga catttatact taagatacaa agaaagatgt cagacatata
     3421 gagagaacaa acggttacag aaaaatagga ggtatgaaaa tgacagagtt tgttgcttcg
     3481 ccggaaagat tgattattgg agcactgtta ggcttcgcag tattactttt tttgatcatt
     3541 aagcttaaga tccagccgtt catcgcaatc atgtcttccg cactgatcat aggcctggtg
     3601 tccggaatgc ccttttccat gatcacggac actctggcag agggtgtcgg caataccttg
     3661 cagaccattg ccatactgat cggtctgggc tccatgttcg gagcgatcct gcaggtatcc
     3721 ggtggcgtgg aggtcattgc ggatacgctg ctcaaggtgt tcggggagaa gaaagcgccc
     3781 tgggcattag gcattacggg actgatcgtg ggaatgccgg tattctttga atccggtctg
     3841 ctgatcctga tccccgtggc atttggaatt gcccgcaaga cgaataaatc cgtatttaat
     3901 tattccattc ccctgttggc aggtctggcg atcggacatg cttttgtgcc tcccacaccg
     3961 ggaccggtgc tggtagcgga aatgctggga gtagagctca gctatgtcat catactgggc
     4021 atcgtcatcg gtattcctgc gatgatcctg gcaggaccgg tgttcgccgg atttatcggt
     4081 aaaaaaatcc attgcccggt accgtcttcc tatctggaaa atgaaaaaga agcagagcag
     4141 aaaaataccg gcaaaaaccc gaatttctgg ctgatcgtat gtatcatcct gcttccgctg
     4201 tttttgatca tcctgaatac gttatcctct gtgatcccgt tcatgaagcc ggcacagccg
     4261 gtcctggaat tcctgggtac tccggtcatc gctcttttac tgtcaacaat ctgcgccatg
     4321 ctgtttttgg gactgcccgg tggcttcagc gtaaaagaga tcacgaacat tatgacgaat
     4381 tccctgcatt cctgcggtaa tatcatcctg ctgatcgccg gcggcggaat gctgagattt
     4441 atcctgcaga actcagggat cggagaattg ttcggtaatt tcgtgggcag cctgcctctg
     4501 ccgatggtgc tggtagcatt cctggtggca gcagtggtca gaatctctgt gggatcggcc
     4561 acagtgtcca tgaccatggc agcggggatc attgcttcca tgcctatggt atccggcttc
     4621 tcacagttac agcttgccac gatcaccatt gccattgcgg caggcggcac agccttcagt
     4681 catgtgaacg attccggctt ctggctgtcc aaatctctgt ttgaggtgga tgagaagacc
     4741 aatctcaaga cctggactgc catggagacc attgtgggta tcgtgggagt gatcggagct
     4801 actatcgtat ggtttgtagt gtcttaaaga aaaggagaga cgattatgct tagaagtgaa
     4861 gaagtgagaa aactggcacc ggagatggat ccgctgcgta tgggaatggg ctggaaggta
     4921 agtgatctgt ccaagccgca ggtcatggtg gaaagctcct acggagacag ccatcctggc
     4981 tccgcccatc tgaatatatt cgtggaagaa gcggtaaagg ctgtggatga aaatggcggt
     5041 aaggcagcca gatattttgc tactgatatg tgtgacggta tcgcccaggg ccacgatgga
     5101 atcaattact ccctggcaca ccgcgatgcc attacgaatc tggtggaggc acaggccaat
     5161 gccacagtat tcgatggagg cgtattcata gccagctgtg ataaatccat gccggccatg
     5221 ctgatgagca tcggacgcct gaaggatatg agtgcgatcg ttgtgaccgg aggggttatg
     5281 gaggcacatg agctgccgaa gaaatatgtg gtggatgatc ctgcctgcag gatcaatgag
     5341 ctccttaccc tggaacagat cggtaaattc gatgcctggg agaagacggg agtcattccc
     5401 aatgaacagc tggattatta taagcagcat gcctgcccca gctgcggagc gtgctccttt
     5461 atgggcacgg cgtccaccat gcagatcatg gcggaggctc tgggactgat gctgccgggc
     5521 accgcactga tgccctccac ggcaccggaa ttacgtcagg cagcctacga tgcgggaaaa
     5581 cagctgatgg agctgatcgc aaaagggatc aaagcctctg atatcgtaac gaaggaaagc
     5641 tttgagaatg cgatcatggt gcatgcagcc atttccggct ctaccaatgc caccatgcac
     5701 atccctgcca ttgcccatga attcggtttt gagattgatg cagatacctt tgaccgcctg
     5761 cacaggggcg ctcattacct gttgaatatc cgtccttccg gagactggcc cgcacagtat
     5821 ttttactatg ccggtggtgt tccccgcgtc atggaagaga tcaagagcat gctgcatctg
     5881 gatgtcctga cggtgacggg caggacattg ggtgagaacc tggaggagct gaaaaagaac
     5941 ggcttctatg aacattgtga tgaactgctt cgcgaaaagt cgaaattatt cggaagaact
     6001 atcgacagag aggatattat ccactccttc gagcacgcca agggtacgga gggtagtatc
     6061 gctatcctga agggcaacct ggcaccggag gggtgtgtga tcaagcatac ggcatgcccg
     6121 aaaaatatgt tcaaggcagt gctgaaggca aagccctatg acagtgagga agcatgcatc
     6181 agtgcagtgc tgcacggaga agtgaagccg ggagatgcaa tattcatccg ttatgagggt
     6241 cccagaggca gcggaatgcc cgagatgttc tataccggag aggctatctg tgcagatcca
     6301 aagctggcct ccagcgtggc actgatcaca gacggaagat tctccggagc atccagagga
     6361 ccggtcatcg ggcatgtatc gccggaggcg gcagcaggag gtcccattgc attcgtggaa
     6421 gaaggagatc tgatcgaact ggatgtggag aacagaaaga tcgccatcgt gggaatccgg
     6481 ggcgagagga agactccgga ggaaatagac gcagtcctgg cagaacggaa agccggctgg
     6541 aagggctttg agagtaaata tcagcatgga ctgttaaaac tgtacgcaca gcatgccgta
     6601 agctccatga agggtgctta catggaataa tatgatgcca gggtcaaaag gtctaagccc
     6661 ctatgaacgg aacaatccat aggcatcaaa gtcgggggct tttgaccccg acatcataat
     6721 tttaattcat atcagaaaac ccaggcacat cagctcagct ggtgtgcttt tttatataaa
     6781 gtttttgtaa ctattcagaa ccccattcgc aaaatcgcct ctacgaggtt tcttttttgc
     6841 tcatgtggtt acttcgacat ataaaactgt ttatctgcct gaatgcaagc attcgcagaa
     6901 cacagttctg catggctctg aatagttaca agttttttat tgaaaagttt acaaaatttt
     6961 acgataatgt tcaaaaatat acaatttatc aaaagtgatg tagacaaaca ataaaaaagt
     7021 gggtaatatg tatatatagt aagctcgaca ggcaaaaagt atgtacaata tgctacataa
     7081 ttaaagcaag aaatataaaa tagagaggaa tgttgtcgat gaaacagttc agctatgtta
     7141 ttaaggatga aatcggtctg catgcacgtc ctgcgggact gctcgccaag gaggcaaaaa
     7201 agtatcagag tacagtgaca ctttcctgta aggggaaatc agctgcagca agcaagctga
     7261 tggcgatcat gggaatgggc gtgaagcacg gagacaacgt ggaagttacg gtagaaggag
     7321 cggatgaaga taccgccgca gcagagcttc agaagttttt ccgtgagaac ctctaggaga
     7381 agacctgcac gacagagttt ggaaggtaga aaatggaaaa atatattgga aaatccgtat
     7441 ataagggaac agcgatcgga cctgtcagtg ttctgaagaa aaaggacagt ctcgttaaga
     7501 gaatccatat cgacaacaca gcggaagaac tgaaacggct ggatgcggct aaggaacgta
     7561 cgatgacgca gctggggcag ttatacgaga aggcgctgca ggaggtcgga gaagtcaatg
     7621 cagctatttt cgaggtgcat cagatgatgc tcgaggatga cgattatcag gatgccattc
     7681 acaacatgat tcagaacgaa gaagtgaatg cagagtatgc ggtggcagtg accggtgata
     7741 attttgcaga gatgttcgcc aacatggatg atgaatatat gcaggcccgc tctgcggatg
     7801 tcagggatat ctccaaccgg ctcgtccgca atctgtccgg ggaggagcag attgactgga
     7861 gtaccatgga gccgtcgatc attgtggcag atgatctgac acccagtgag acggtgcaga
     7921 tggataagag caagatcctg gcctttgtca cagtacatgg ctcaacgaat tcccatactg
     7981 cgatccttgc acgtatgatg aatattccgg cactgatagg ggtaccattg gaactggaga
     8041 aaatccataa cggaacccgt gctattgtgg acggcaggga agcggtattt tacctggatc
     8101 cggaggaaga gcagctccgg caggcagaag cggcacagca gacagagcag agacagagaa
     8161 gcctgctggc agattacaag ggacgggaat ccgtgacaaa gagcggacgc aaggtcaatg
     8221 tatatgccaa tatcggcagc gtatcggatg tggcatatgt actggagaat gatgcggagg
     8281 gtatcggcct gttccgcagt gaattcttat atctcggaag ggattctctt ccggatgaga
     8341 cagaacagtt caatgcttac cgtcaggtgc tgcagatgat ggcagggaaa aaggtcatca
     8401 tccgtaccct tgatatcggt gcggacaaaa atgtagatta cctcggactg ggcaaggaag
     8461 ataatcctgc catgggatac cgcgcgatcc ggatctgtct ggagcagccg gagatcttta
     8521 agacgcagct gagagcactt ctcagggcgg caaaatacgg aacattggct attatgtatc
     8581 cgatgatcat ttccgtggag gaagtgttgg atattcagaa aatcgtggct gaggtggcta
     8641 aggagctgga ggaagaaaag attccttacg caattccgga acagggaatt atgatcgaga
     8701 ctcctgcggc tgtcatgatg agtgaggagc tggcggagca tgttgatttc ttcagtatcg
     8761 gcacgaatga tctgacacag tatacgctgg ctatcgacag gcagaacaac agactggaca
     8821 gattctataa tccacatcat gaggcagtcc tgcgtatgat acagatgact gttgacggag
     8881 cgcataagca tggaaaatgg gtcggcatct gcggagaact gggagcggat acgacactga
     8941 ccacacgatt tgtgcagatg ggaattgatg aactcagtgt ggcaccgtcc atggtactga
     9001 atgtccgtag taaaatatgt gaaatggagt gagagattca tgagaatata caaagcgaaa
     9061 gattatgcag atatgagcag aaaggctgca aatatcgtat cggcacaggt tatcatgaaa
     9121 ccgaactgtg ttctcggact ggctacggga tctactccca tcggcctgta taaacaactg
     9181 gtggaatggt tccgcaaggg agatctggac ttttctgagg ttatgacggt gaacctggat
     9241 gaatataagg ggctgagccg ggaaaacgat cagagttatt attattttat gcaccagaat
     9301 ctgtttgacc atgtgaatat cccggtggaa aatacacatc tgcctaacgg aatggagccg
     9361 gattcggaga aagaatgccg tagatatacg gaattgatcc agtcgctggg cggtgtggac
     9421 ctgcagcttt tgggaatcgg acataacggg cacatcggct tcaacgaacc gggagagagt
     9481 tttgacaagc aggtacattg cgtgaacctg acagagtcta cgatcgaagc caacaagaga
     9541 ttcttcgcat cggctgagga tgtcccgaag caggcatata ccatggggat caagaccatc
     9601 atgcaggcga agaaaatatt gatcgtagcc agcggcgaag acaaggcgga gatcgtacag
     9661 aaagctttct tcggacccat tacaccgcag gtgccggcat cggtattaca gcttcataat
     9721 gatgtaacgc tagtggcaga cgaagcagca ctcagtaagc tttctgaata agaaagggga
     9781 agctgatagg tcatgagaat cagaaatgca aatatatata cagaggagca tcggtttgtc
     9841 agaggggatg caaccgtgga gaacggcagg ttcgtaagct gtaccgggga tccggcaggt
     9901 gaggaagctg ttgtggatgc ggaaggcatg tatctgatac cgggactggt ggatattcat
     9961 ttccatggtt gtaagggcgc ggatatgtgt gacggtacga aggaagcact ggatgtcatc
    10021 acatcatatg aagcctccgt cggtgtgaca agtgtctgcc cggctaccat gacgatccct
    10081 aaggatgaac tgctggaggt catgaaaaat gccggcagct atgagtattc cggtggatcc
    10141 cacctggtgg gcatcaatat ggaaggacct tttatcagtc cggcgaaaaa gggtgctcag
    10201 gcggcggaaa atatcatgca ctgcgattat gaatatttca gccggctgca gaaagcagcg
    10261 aatgggctga tcaagctggt agatatcgca ccggaggagc cgggagcgat ggaatttatc
    10321 gacagagcca aaggagaagt ggtcatttca ctggcgcata cagcttccgg ttatgataca
    10381 gcaagggaag ccattgaaca tggagcatcg catgcgacac atttatataa tgcaatgccg
    10441 ccgctgaacc acagggagcc cggcgtgatc ggagcagtga gagattcaga gaaatgtcat
    10501 ccggagctga tctgtgatgg tgtacacatt catccgtcag tgatccgtgc aacatttgcg
    10561 atgtttggtg ctgaccgcat gatcctgatc agtgacagta tgcgtgcaac aggcctggag
    10621 gatggtgaat atactcttgg aggacagccg gttaccgtca gaggcaatct ggctaccctg
    10681 catgacggca cgatcgccgg atcggcgacg aatctgatgg attgtatgcg gtttgtagta
    10741 aaaacggtcg gtctgccgtt agagacggca gtgatgtgtg ccacggagaa tccggcgaag
    10801 gagatcggca ttttcaatga agtgggcagt atatccgttg gtaagagggc agatttcgta
    10861 ctcctggatc aggaactgaa cattgcagcg gtatatatcg acggaaagga gttcacatgt
    10921 tagatttttt gaaaggtaaa cgaaaaggga attgtatcgg atctccctgt aaggggaagg
    10981 cagttgctct tacggaggtg ccggatccta cattttccga gaagatattg ggagacggat
    11041 ttgcagtgat cccctctgag gggaaaatat atgctcccgc agacggagag gtgaccgtgg
    11101 tatttgatac gcttcatgcg atcaccatga caacagatca gggagcggag cttctgatcc
    11161 acatcggact ggacaccgtg acacttaagg gagccccctt cacagcacat gttgctgccg
    11221 gagaccatgt caaaaagggc gatcttctgc tggatgcgga tctggagaag attcaggaag
    11281 ccggactgca tatcatcact ccggtcctgg tctgcaatac cgatgaatat aacaagatca
    11341 gtattctgaa agagggagag gtatctcccg gcgaggacgt gctccagatc tcttaagagc
    11401 ggtacgtccg tatcaactaa cgaaactgac cggaactgtt ccggttgagt ataatgtaaa
    11461 ggaggagaaa tgtatgaagt ttcttcaaaa actgggtaaa gcgttaatgc ttcccgtagc
    11521 ggttctgccg atctgcggta tcctcatggg tattggttac aggttgtgcc cggcaacaat
    11581 gcagggtggt gagatctcgg gcgtggcaaa tctgatcggt ctgttcctcg taaaagcagg
    11641 tggtgctttg atcgacaaca tggcgatcct gtttgcaatc ggtgtaggtg tcggtatgtc
    11701 agagaaaaat gacggtaccg gagccatcgc agctctggct tcctggctga tgctcacaac
    11761 acttctgtct accggatttg ttacgaccat tatgccctcc atcgctgagg atgctaccaa
    11821 gacactggca ttcgataaga ttgcgaatcc ctttatcggt attattgccg gtattatcgg
    11881 ttccatgtgc tacaacaagt tcaaggatac caagcttccc gactggctat ccttcttcag
    11941 tggtaagcgc tgtgtagcta tcgtagcagg tgtggtatct attctggcat ctgttgtact
    12001 gctgttcgta tggcctttac tgttcggcgg tctggtagct ctcggtgaag ccattgtagg
    12061 tctggatgta gtcggtgccg gcatttatgc tttcctgaac cgtctgctga tccctaccgg
    12121 attacatcac gcactgaaca acgtattctg gttcgatacc atcggacttg gagatctgaa
    12181 gcacttctgg gcaggtgaga caagcgctga tgtatcctgg agccttggaa tgtatatgtc
    12241 tggcttcttc ccctgtatga tgttcggtat ccccggtgca gctctggcaa tggtaaaatg
    12301 tgcaaagccc gccaagaaaa aggcagcgat cggtctggta gcttccgcag caatctgttc
    12361 cttcatctgc ggcgttaccg agccctttga gtttgcattc atgttcttag ctcccggact
    12421 gtatgtgatc tatgcagcac tgtacggcat cttcaccatg atcactgttg ctcttggctt
    12481 ccgtgcaggc ttcagcttct ccgcaggtgc gatggatctg tttttctcat cctccctgcc
    12541 tgcagcagcc agaatctggc tgatcatccc tctgggtatc gcagcatttg ttgtattcta
    12601 cttcgtattc ctcttcgcta tcaagaaatg ggatttgaag acccccggta gagaagatga
    12661 tgatgtagaa gcagagaaga agatcgagct ggcaaatgat gatttcgcag caatcgcagc
    12721 aaccattctg gaaggctgcg gcggcaagga caacattgca agcattgaca actgcattac
    12781 aagactgaga ctggaagtaa aagacattac cgcagtggat gataaggtga tcaaatctgc
    12841 aggcgtagcc ggtgtcatga aacccggtaa gacctctgtt caggtaatca tcggacctaa
    12901 ggttcagttc gtagcggatg cattttccaa gctttgcgaa taataaagta ctgataaaac
    12961 tgaatatatg aacggaagag agacctgcat gggaagacca tgcgggtctt tcttatatat
    13021 gatgtcgggg tcaaaagccc ccgactttga tgcctacgga ttgttccgtg ctatgcactc
    13081 tcacaatcct accctctatt cgcatatcgt gggattatca tcccactttt atgctcatat
    13141 cggggcgggt cataaggtct ttcacccact tatgagcaag ctcatgtggg cttagacctt
    13201 ttgaccctgg catcatatat atttaataat tactttattg acatttactt ttgaaatgat
    13261 aaaatatgtc ggagaaaaaa agctgcaggc ccgatgcctg cgggatgtat tgtaatgtaa
    13321 tggagagaaa agtcatgaaa aaatcaacag gaaagagggg aaacggggga ttttcattag
    13381 tagagctgat cattgtaata gctatcatgg cagtgctggt gggagtactg gcaccgcagt
    13441 atctgtctta tatacataag gcaaaggtgg cggcagatca ggcgaatctg aagaattatt
    13501 atacggagat tcagctggat tatgcttcta caggagtgta taatccgcag gtgccgctgg
    13561 tggataatat tggagaaggc ggttatgaac tcagagagat tcacttttta agtgggcaga
    13621 ctgtgaagct gaaggcagga tactatgctg tgacagaaaa caccaggggt cagggaggtt
    13681 acaatatatg ctattattgt gatctgtgca cagctgctga tgcggatgaa cagacaaagc
    13741 aaaagcatct ggatacttgt gtcattacat ttctgtgatg gcggctgata ggaatatgtt
    13801 atttttccag acataaccca atattatcag aaatgcagca gatgtatcca tgtttcgcgg
    13861 cgtaggtggc tgcttgtctg cggatggcag gaaccgttgc gtattccaga gtataagcgg
    13921 tacagccgtg tgacatagca aaatctaaat aatcagtata gtattcacgt gtacttttat
    13981 cgtttatggt ataggtatca tttgaaaaat cgtacgcagt aaaaacgttc tcctggttta
    14041 ccccatcgat gagaacgttt ttttcagctg taagatattt ttttacaaaa cagtccccac
    14101 cgttgatcat gatctttctg cctgtatggt tcatatagtc cagaattgta aggattccgt
    14161 catagatgga ttcctgcgga tagttatagt atacatccgt gttatcgaca aaaaagccgt
    14221 ccacaccctt ctgtaccagg gcgtctactc tggaagctgt acatgcctgc cattccggag
    14281 ctgagacatc gatccatttt tcctccggcc aattctcata ggcacccaga gtatatttct
    14341 caaagtctgt atcataggac cggaagctct cgatcgatcc gatattgata taggagtaga
    14401 tttcatggat accattttct cttaaacggg caatgtcatt ttgggaaaaa tattccgcat
    14461 ctatgacaag cagatcaatg tcagaaagtt tttctgtgat ctcagggtct tcgccgatca
    14521 ggacaatgta tttttctgca ctattgaagg tgctggtgga gcaggcactg agcagggctg
    14581 tagataatat cagaatgaga acacatatta tttttatgaa tagcttcgtt ttcctcaatt
    14641 aacggtacct cctttttcgc ttgatctgct ccaatatttg tatccagata attatactat
    14701 agatggctct acatttcatg tatttctgtt aactattcag agccatacaa aatactgcat
    14761 tcaggcagat aatgtcgaaa aaaatatctg taaaaatatt aaaaagggcg aaaaatattg
    14821 aaaaatgtgg tataattttt tatgcaaata taagataata ccgggacaaa aagtccccgg
    14881 atttgatgcg ttcggcggtt aagataaccg ggaaagtagg aagttatatg aagattcagg
    14941 atttttgtga catgaacaaa ttcgagcaga tcatgaagaa ctgggcaaac agcacgggac
    15001 tggcaaccgt ggctgtgggt gcagacggaa aatacatcag tgactgttat aattttacgg
    15061 agttctgtat tgatctgacg agaggaagcg cagaggggaa aaagcgctgc gagcagtgtg
    15121 acagagaagg ccatggtgtg tatccctgcc atgcgggact tgtggatttc ggcattccga
    15181 tcacactgga ggatggaacc gtgctgggca gtattatcgg aggacaggtg ctgccggaga
    15241 atccggatga ggagaagttc cgtcagacag caagagaact tggcattgat gaggataaat
    15301 atatcaaggc cttgaaaaag gtaaatgtaa agacaaggga gcagatcgac gcttcggcaa
    15361 atctgctggg agatgtgatc aacatgttcg taagagccag ctacgtcaac aggaaaaacg
    15421 agaatctggt aggtgagcta aagggtggca ttacgaaagc tgcggaacag atcgaggaag
    15481 caacggacaa gaccaaggaa atcgatggct acagcaagag acagcagatt ctggcactga
    15541 atgcttcgat tgaggctgca agagccggag atcagggcaa gggctttgcc gtggtggcaa
    15601 cggaagtaca gaagttagca agagatatgg cgacctccag tgcggacatt aagaagctgt
    15661 tgggagagct gcatgttacg atcaaccatt tgaatcaata gtaatttagg ataaagttta
    15721 caggaaaacc gtttttctga tccacaaagg gtgcagaaaa acggtttttt ttttgctttt
    15781 tgcatgtttc cttttggtgt gtataaaaaa gtcggaggag tggggcgata ttctccatag
    15841 aggagccggt aaactaacgg agttggcatt tgt
//
LOCUS       DAWDHD010000049        15712 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_253_length_15712_cov_10.702944, whole genome shotgun sequence.
ACCESSION   DAWDHD010000049 DAWDHD010000000
VERSION     DAWDHD010000049.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 15712)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 15712)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15712
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_253_length_15712_cov_10.702944"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3542 a         4499 c         3294 g         4377 t
ORIGIN      
        1 aaaggatctg atcgcagcag gacagttcga ggaaggcacc atgcttccca agatcgaggc
       61 agcgatctct tacctggaga aggttcccgc cggcaaagtc ctgatcactt ccatggatca
      121 cgtcaaggat gccatcaggg gcaaggccgg caccgtgatc accgcttaat tttatatcgt
      181 aagcagttaa ggcaacacaa aaggagatat ccgccacgga tacctccttt ttacatgtca
      241 tgattctaat atctctgggt tctatatctc tgatctctat gcctcttttc cagtcagatg
      301 gttcgacagg atattataca atgtcttcgg gttgatgggc ttggacaggt gatagttcat
      361 tccggcctcc agcgtctttt tcttctcctc ttcaaaagca tccgctgtca tggcaatgat
      421 gggtaccgtt tgcaatctcc accacacatc cggctttttt caggatcagc tgggccacat
      481 aaatgttgat ctcgttatct tccaccagta aaattctggc atccatcaga ttctccgtga
      541 tgttttcctc ggaacgctcc tgggagaacg gatcgtacag gtgcggcagg aactccggac
      601 tcattccgat accgttatcc ctcacacgaa accggatttc caccttgtca tcctacttca
      661 tttctacacc acagatacaa taccaacatt ttctttttcg ttctgcatac catgccatac
      721 ttaacttata ctctccctct aaataattgc tatctctgta tagatgtact tctgtaataa
      781 acattcctag gccagccatc tacaacaaac tgatcggcaa atttatagcc tgtactctct
      841 atgctacata cagacggagt attattagga tgagcacaac accaaatagt atcataaccc
      901 atctgtctag cttctcttat agtagcatcc atgaatctct tctgtatacc attacctcta
      961 tattccatat caaccacaac gaattcatga cacataattt tattgccagg aatttccatc
     1021 caatcagtaa taggcttaga gaagaacaaa ccaccttcaa atgcaaagct tgctgcaata
     1081 agcttatctt tactatatac gcctaataag atataatcac catccaaaca ttcctgtata
     1141 gttctttcat ctgtaggata ataatattca tcatgtagca aagtcttatt aacttcatcc
     1201 tgtaatttaa taacaggttt caagtctctt tttggtttct ttatctcaat ccttcttatt
     1261 gataaatcaa aatattgact catatgaaac ctccatttac tccggctttt ttactcgtat
     1321 ttttccagat agaaggaata caggatcctc tccttcacgg gcagctgcat cagcttcgtc
     1381 agtgcctcct catagtattg gatctggacg ctgtagcggt tccagagggc ttgcagggaa
     1441 tcgatcacat cggtcttgta gtccaacagg acgatctctc cgttttctat aaagaagaca
     1501 tcgatgatac cctgaatgag aaccttttcc ccttcaggaa ggtcctgatc cagacgcttc
     1561 gcatcaatac ccagcacgaa gggctgctct ctgtacaaaa ggccctgttc gaaagctctc
     1621 cacatgcggt aggccagctc ctgttccaga aaatgccgga ttttgggaag gcttacggcc
     1681 tgcgcatact cctctgtcag tcgtagggaa gctgtctccc gctgcaggaa ttccttcaga
     1741 cgtacctgta accggtccgt atccaggccc tgacggtatt cctcataagt cccagggcat
     1801 ccggcaaata gtccggattc cataaacaga taagaaaagt ccaacagttc catggtccgg
     1861 tggaacgcgt taccgcggac ggctccgctg accttttcct gttctctgcg gaaagccggg
     1921 atataaggca ccacttcttt ttcttcgaag gtatgaaatg ccgcttcatc cttctccgcc
     1981 atggccgcaa tcttcagttc cgataccgtg gtcttggtat agagcttctg caggccggga
     2041 tacggatagg cataggcaaa tcgctcccgc agtttcgcaa gctttcgttc gttctcctcc
     2101 gggtccccag gcaaaggagt ctcgccgtcc gtcacacgct gcaacagttc cctgcggtct
     2161 cccatgcgca gttgttccct gatctcctct gccaccaggt cttctgttcc cagcgttgtt
     2221 actgcaattc ctgtcttagg caggatcggc agcagaaaat ccatatagct ccccgcctca
     2281 cagaaatcca gataggtcag cttctcctgt cctgtcatcc gggacagctc ccatttttcc
     2341 tccgccttat ccaacaccgc tgtcaggatc agtttttctc tggcacgggt cagtgccacg
     2401 taaagcacac gcagttcctc cgccagattg tcctccttca ttttcgctga cagaacggcc
     2461 cggcggaggg tcttattttt aatgcggcgg ccggaatcca catagtccac cgctactccc
     2521 agatccatat ccacgatcag agactggttc gcatcctgca tgttgaaacg cttcgacagc
     2581 ccggagacaa ataccacggg gaattccaga cctttactct tgtggatgct catgatccgc
     2641 accacatcgg cattttcgtc cagggtatcc gcctcaccgt aatccacatc gtatttttcc
     2701 aactgttcca tataacggat gaaatggaac agtccaaaat aactggtctt ctcaaaagca
     2761 gaagccttcg tcagcagcat ctccacgttg gcccggcgct tgctgccagc aggaagtgcc
     2821 gtcacatagt tcagataatc gtaatcgtcc aggatccgct gcagcaggtc gcggatggag
     2881 gtgaagggag tcagatcacg gtaatgatca atacgttgta agaaagtatc tgccttttgg
     2941 gaaagttctg tctccggttc catgtcctcc ggtactgttg cagcttccaa tgcgcgctgc
     3001 cagtactgtc cggccccggc ggtttcttcc ccggcgcttt cgcccgctga tgcctcttct
     3061 cccgcttccg ccatccgtcc actctgcgcc acttctttca atgcctcata cagtgtcatt
     3121 ctcttccggg aatgcccttt gcttccgctg cggatccggg cgatctcttc ctgggtaaag
     3181 cctccgaaga tcgactgcat cacgccgaac agcggaatat cctgtctcgg gttgtccagc
     3241 actcgtaaaa actgcagcag ttcctgtact tccagggccc cgaaatatcc ggtcttggat
     3301 gtaatgtaga cagggattcc ctgctgttcc agtactttct tgaattcctc atcccagcca
     3361 ctggtggtac gaagcaaaat caccatatcc gagtaccgca ccgggcgcaa ttctcccgta
     3421 gccttctcca tgactttcaa actgcccttc agctgcttga tacgtgccgc aatagccagt
     3481 gcttccagct gtcttacgtt gtcatagtcc acgggacctc ttgccccgtc ggggcgctgt
     3541 tctccgctgc cgccttccgc accatccccc ttctccggct tacggatcag taatagttca
     3601 ctgccgtatg caggatcctc tgtggcggga tatgtggccc cgggatacaa tgctgcctta
     3661 tcatcatagg caatgccgcc gatctcccgg ctcatgatgc gggaaaacac atcgtttacc
     3721 gcatccacca cctgcacacg gcttcggaag ttcttcgcca ggtcgatccg gcacagatcc
     3781 ccactctcct gataggtgtc gtatttttcc aggaaaagtt ccggtctggc cagacggaat
     3841 ttataaatac tctgcttcac atcacctacc atgaagcggt tataatgttc ctccgcttcc
     3901 ccggagatgg cacttaacag atattcctgt accagattac tgtcctggta ttcgtcaatg
     3961 aggatctcct gaaaatactg gcgatactcc aatgccacgt cacttggcac aatgtcatat
     4021 ttcacaccgg tgctgtccgt ccctgcatcg ccggtctctt ccactttttc ccgttttagc
     4081 agaatctgta acgcatagtg ctccatgtcg gaaaagtcga tcagatgtcg ctcctgtttg
     4141 gccgcaagaa gccttctgtc gaattccagc accaggttaa gcagcatccg gagcggctcc
     4201 ctgcaggctt ttccctgttc caccaccaac tctaacggtg tcttgaaata ttgttccgtc
     4261 agatccgcca gcgtgtcttt tacgctatta cggatggatt tcgccagctc ccgtttggcg
     4321 ggatccacgc tctcatcctt cttggaagaa agacggccga aagtcactgc tggaaccttt
     4381 gctgcctgtt ccttcaaatc cttgcaggca gccaggcgtt ccagctgttc gctctccgct
     4441 tctgtcagtt ctccatacat atatggaccg tccggcagct cacacagctt tttcacttcc
     4501 tccagttttt ccagacaccc ttgcaggaca cggctcacac gctctgccag atattgtccg
     4561 taatcactgt gcagcagggc atccagatct cccgccgcat aatcgttttt acgctcttcc
     4621 agccattctg cgggccaggg aaaacttccg gcatagcggg acaggttcag gatatgctgt
     4681 tccagcacgc tctctctgcc gccggggcag aaatattcca cacaggcgtg gaaacgttcc
     4741 cgtgcctccc gggataggtc ctcgctgtca cgttttcccg gaacttcttc cgcaaaatca
     4801 ttccctgacg cttcctccgg tatcatcgct ccgctgtcac ggttttcccg ggacatcgga
     4861 acatcatccg gaacaaattt ccccgcatag gcatcctcca gcagctgtcc caacacctcc
     4921 tgctgcaaca gcttgatctc accctcgtct gccacacgga aatccggatc cagaccgatc
     4981 tcattaaaat gattccggat caggaacagg ctgaagctgt cgatcgtggt gatctgggca
     5041 ttgtgtaaca gggcggactg cttctggata tggtcgttcc ccggatccgc ttccagtctg
     5101 gcggcgatcc cggctgcgat acgctcccgc atctccgcag ccgccgcatt ggtgaaagtc
     5161 acgatcaaca ggcggtcaat gtccgccgga tgctccccct cacagatcat acggatgata
     5221 cgctctacca gtaccgccgt cttaccgctt cccgcggccg cagataccag aatgttgctg
     5281 ttatgcagtt cgattacctt ttgctgttcc ggggtaaaat tcattcccat acgctacctc
     5341 tgttcctgtt tcttttctcc tgttgccgtc ctgtttctcc gggcagcggg cgctttctct
     5401 tcgagcttcc tgtcaggcac acactcccgc cgggcaccct ttgccctaag cttccacggt
     5461 cttctgcatc cgctgcatca gcgcctgctt atccagatct tccaactgtc tcttctcata
     5521 gcccggaata ctgccatcga aaccgcagac ctttttatag gcgcaataag tacaagcctc
     5581 ttcattgcct ttctcatagg gattggctgc tatttttccg tccaggatct cccgcccgat
     5641 gctgcggatc ttggcatcta cataagaaga tatcagctgc atctcctccc ggcttaagac
     5701 accggatctg gcactgaagc ttccatcctt tttcttctcc accggaatga ccacagactt
     5761 atcctgcata tagcggtcca gccgctccac gacctcagga tcactgttta ctacaccgtt
     5821 catacgaagc tttgcaagga tctgttcatt gatctgctca tccgtgagtt ccaccggtgt
     5881 ctctatggtg ggatcatcga tgtgataata cagaagtgcc gccggaacga tctccttgtc
     5941 cggatgcttt cggctctcca tctccatggc tgcattcata tataccacca gctgcaactg
     6001 cagaccgtaa tacaatgccg ccagatcaaa tttccggttg ccggacttat aatcaatgac
     6061 cttgacgtag acatgctctg catcttccga cacatcgata cgatcgatcc gtccctgcaa
     6121 atgcatcttc tcgtcctcag acagatccac atgaatgctg tccagatcct ccgcaaaccg
     6181 gaaagaaagt tcataatcat ctggctggaa actgccctgt ttcaaatgtt tctgcaaggt
     6241 cagcactgtg cgggtcagga tcctgctcat gcgggtaatg gcatattcat tccgcgcaga
     6301 gctgtataat accgtctccc cgtaggttgc cgcataggct tccacggatt ctttgacggc
     6361 tttggctgca aaattctccg taaaatccca ccatgtcagg ttggattctg caagttttcc
     6421 tgcaaagttt tccagtactg catgatatac cgtacccata tccgaagcct cgaagccgaa
     6481 ttcttcccgt tcctgcaggg aaagaccata ttgcaaaaag tgtctgcagg cacaggcagc
     6541 ataagtttcc agacggctga cactgttttc cagctgctgt ccgtacaatg ctctggccac
     6601 gatccgggac agaccgctct ccctgtatct gcgaaatgcc gcccgggtca gcaggtctct
     6661 gcccgccgca tccgcctcgt aggcacggta catgagatag aagtcctgcc tctcttcttc
     6721 cggcagggtt ccttccacat attcccgcag ttcctccgcc agatatctgg caccttcctg
     6781 tctgccctcg atctgttcca gacggctgcg gttctgggga tactccacgc tcattgccgg
     6841 gaacagcttc cgcaccgtat cgatcaggta ggacggtctt atgccctttc cttcactgtt
     6901 taccttggca taggacagat acagctgttc tgagggcttg gtcatattca gatacagata
     6961 taatctctgg atatacatct gctgtctcgg gctgggtgcc atctctgtcc cggattcaat
     7021 caggaattcc cgatccatat ccgaaatgat tccgcccttg gaagcatttt tcggaatatt
     7081 tccgtcattg actcccagga aaaacaatac cttcacctgt ttcagacggg ttcgctccat
     7141 atcgcccacc acgatacgat ccacgttctg agggatcgta ccgacagtga tctcgccgaa
     7201 accggcctct aaaatatccg caaattcctg tctggagatt tcttcctctc ccaacagatc
     7261 atagacctgt tccagcagat ccatgtccag ccgttaaatc tgtgcatact ctctggctct
     7321 ggataagtct ccctcttttt caaactgctg ctgatagttc aaaagctttt gctgcacctg
     7381 attctgttcc aggaaatcgt acagatggct gacatagtct ccagcctttt cctggcttcc
     7441 catgtgcaga atctccaccg catccatgaa ctgttgccgg atacggttca gacgctccat
     7501 ggtcttctct tccgcctgtt cgctcccctc cgcatttccc tgcatttctt ccgttctgcg
     7561 ggtaaacaga cggctatagg tccgatagcc tctcgctccg gtgcggatca catagttttc
     7621 cagctcatca atctcttctc tggaaatatc cgccatgccg ctgcgcagaa aatgaaacac
     7681 cgtatcatag gaaaagtcct taatatacag ttgcaatgcg ctcttgatat attcgatcat
     7741 gggattcagc acgatccctc tggtacggtc caggaaacat gggatctcca gctgtccgaa
     7801 ttctgtttcc acataggaag cgtatccttc cagatctccg atgaccacgg caatgtcccg
     7861 gtaggtcatc ccctgctctc tgatcaggtg ccggatgtac agcgctgtct ggtggacttc
     7921 ctctctgggg gaaagggctt caaacatatg gatctcttgc tgttctcccg catacggttc
     7981 atactgatac cggaacagat tctgttccag atagtgtaat gccggtgcct tggcaaagcg
     8041 attgggcccc cccttgacaa agagatcctc tccccgctct acctctgcct ccgctgccag
     8101 cttttccaga tccgccaccg tcttcttgct cagatggaac agcttctgtt caccatccat
     8161 tttatacgga tcctctccca ctccaatggt cacggtaacg atggtctctg cgcacacgcg
     8221 catcagttcc tggatcagcc ggttctgtat gggagtaaat cccgtaaagc cgtcaaacac
     8281 cactacactg ccctttaaga tctttgattt ggacagggat ctgcgcagta catccagtgt
     8341 ctcctcggtg gtgataaaat gatccctgat ataatcctga aagccccggt acagagtctt
     8401 taagtctttc agtttcatgg ccagcgcgcc acgcttctgt gcactggtga tcagcttgtc
     8461 catatcctgt gtgctgatac cgtattgcat aaactcggag atcgcacttt tcacctcatg
     8521 gatatagccc tgcttgtgca ggagacttcc catggccgga agctgttcct tcagatccgc
     8581 cgccactttt tgcagcacca ggcttttgcc ggtgtcatcc agtaccggca tctctttggt
     8641 ccccacttcc tccagaatgc ggtggctgag tcggccgaag ctcaggacat cgatattcag
     8701 gatgccatcc cggtcactgc gcatgaccag atccttctgg gtctgcatgg tgaactgatc
     8761 cggcacaatg atcaggaaat tcctgcccgg ttcctctgcc gcccgctgca tgatctcttc
     8821 atagattctg tggcttttgc ccgacccgga agggccaaaa cagaaacgta agctcataag
     8881 tctcctccgt tatgctgtcc gtttatttca gatccagttc aatgggacaa tgatccgatc
     8941 ccaaaatttc cgtgtggatc ttcgcatccg ccagccggtc tttcagacac tccgatacca
     9001 cgaaataatc aattctccat ccggcatttt ttgctctcgc ctggaacata taagaccacc
     9061 aggaatacac cccggtcagg tccggataaa aatagcggaa cgtatcgata aatccgttct
     9121 ccagtacctt cgaaaagcag gctctctctt catcagtgaa gcccgcattg ccacggttgg
     9181 tcttcgcatt tttcaggtcg atttcctgat gcgccacgtt caggtctccc gcatagatca
     9241 ccggcttttt ctcttccagt ttcttaatat acgccaggaa atccgcttcc cactgcatgc
     9301 ggtattccag tctcgtcagc tctctctggg cgttcggtac atagactgtc acaaaataga
     9361 actccgaata ctctgcagtg atcacccggc cctcatggtc gtgttcctcc acgccgatgc
     9421 cgtaagtcac ctgtatgggc tcttcctttg taaaaattgc agtgccggaa tagccctttt
     9481 tctctgcgta attccaatac tgatgatatc cgggcagatc cagtgcgatc tgtccctcct
     9541 gcaattttgt ctcctgtaag cagaaaatat ctgcatccgt ttcctgaaag aaatccagaa
     9601 atcccttctg cacgcaggct ctcagcccgt tcacattcca tgagatcagt ttcatatttt
     9661 ccctgcctct ttcctaattt gtcttatgcc atcttatccc ggtcataatt tttatccgct
     9721 tttctgtcat ctattcgttc tatccgccgt gcttttccat ccattcacat tccttccggg
     9781 cagccttcag agacgcttcg taccctttca ggatctccat aagtctctgt acatcgatga
     9841 tgccgctggc tttattttcc gggtgatcca tccatggatg ctgtttttcc ggcggataaa
     9901 tattttctat ggtattttcc ccgatccgat accatttctt ctgatagcag atcgtgttgt
     9961 ccgccgggtt taaaaaggtt tccagttccg tcagtggata gatctctgcc ggtgtccggg
    10021 gattaccttc cagacctctc acggcagtga ccttcacagt tccaccctcc ggtactacac
    10081 acagccacat gggctctgcc ggttttttcc ataatttatc cagtataaag ttcaacggtc
    10141 ccagtaacag gatcgtcatg aaaaccgcca gcgctcccgt cagttccgga atccagtcac
    10201 cccacaacag catctggaac agcagatggt cactctcccg cacaccccag accttcatca
    10261 ctaccagcac ggttgctacc atatagatca gtatagtgat cagggcaaac ggcatcagta
    10321 ctttttcttt tttcatgccc catgcggcat aagccttgca ttcatcgtcc gtaaaaagca
    10381 cgggcagttc atagctctct gtcatgctcc gcttctccct cttttgctat tctccgagaa
    10441 taatatgcag gaaccgcttg tccagcatca acgtccggtt ccagggattc aggatcacac
    10501 cctcgatacc atcggcagtc aacgtcatgc ggaacagctg ctccatatcc gtgagaaaag
    10561 tggacattac gctgccgctt ccctttaatt cttcctcaaa tccggtgaag gccgaccacc
    10621 attttttccc atcggaggtc tgcaccgcct gcaggcgcat ggcagttccg gcggcctgtg
    10681 tcaccgcttc ctgcacatct gctgagggcg gttccaccgc cacgataagc tgtccctgct
    10741 cccgcatacg tctgcgcacc accgtcagag catgcgccag aagttcttct gtcggctcct
    10801 gctgtaatgc cagaattgcc tcctctattt tctcatttcc ctgtaaacct ttatctttct
    10861 tttcgtccat aaccgtaatg ttgtcctttt taattgtagt cgttaaatcg cttcatcctg
    10921 tttcccatgt cccttacgca atcttgtatg caatccgtcg aattccataa gagccacagg
    10981 caacacagct catgcctatg cccatcatac cataaatgcc tccgcaattc cactcacgtt
    11041 tcccttcaga agccgtcttt tccttgacag tggggatatt cgttgctaac atgaacctga
    11101 gcgtttgatg cttagaaatg aggataatta tggatacttt tgacccgaag cacatttaca
    11161 gcgatgccgc aaaactggct cgtctgaaag ataaaaagct tttcctgttc gatatcgacg
    11221 gcaccattgc cgtaggcgat accttgtatg agggaagtgc ggatcttctc cgctacattc
    11281 aggccatcgg cggcagggct tattacatta ccaacaattc cacaaaaagc ggactggact
    11341 atgtgaaaaa attccacgat gcatttcacc tggacagcac ggaggatcag ttcatcacct
    11401 ccggctatat gacgctgcgt tttttgaagg agaattatac agacaagaaa atcttcgtgc
    11461 tgggaaccgc ttcttttgtg gcggaactgc gaaagaatgg cctctgggta accgaggtct
    11521 gcgaggacgg cattgactgc gtggtcgttg cctatgacag tgaattaaat tatgcaaagc
    11581 ttaccgaagt ctgcaaggtt ttattcaccc aggatgttcc tttctacggt accaacccgg
    11641 atttgcgctg ccccatcgac ttcggcttta tccccgattg cggtgccatc tgcgacatga
    11701 tcaccgccac cacagacaaa cagcccatct atctgggcaa gcccagcaaa gaggtcgtaa
    11761 agctttgcct tgaagtctcc ggcttcactc ccgaggagac tctggtggtt ggcgatcgtc
    11821 tgtacaccga tattgcctgc ggcatcaacg gtggtgtgga cacctgtgtt ctcttcaccg
    11881 gcgaggcaca aaaaaaggat attctgactc ccgaggggga gccgaatacc caatatctgc
    11941 cggactatgc ttttgaaaat gtgcaggaat tattaaatgc ctgccagggt gaaataaatt
    12001 ttaaatagct ctcttcacga caccgattcc caacggataa ggcccggtgt gtacgccaat
    12061 ggtggcaccg atctggaagg tgggaatggt gtcgatgtcg tatccctttt ctttcagtct
    12121 gcctgccagt ctgtcgcgga actctacggc ttcttcataa tcgtagccat atcccacgca
    12181 gacgctgtaa ttggcaatat ctgccttttc ctctgcaaga tatgctaaca gcagatcgat
    12241 atttttatct accgtcttgc gacggcttct gccgataccg gaggggaata tctctccctc
    12301 ttttaaggta atgatgggct tgatgccaat caggctgccg gccacgctgg ctactttgcc
    12361 gatacgtccg ccatgcttta aatattccat atttcctacg gtgaaaaaga ttctgccgga
    12421 gctcttgatc tcattcaggc gatcgatact ctcctggtaa ctcttccccg catctctaag
    12481 gtgtaccgct tccaacacat agatgccctg caatacggta ttcacagtag agtcgcagac
    12541 ataaatctgt gcattgggat attcctcttc caggatcgct tttgcattca gagcagactg
    12601 catggagccg ctgaacttgg ttgtgataca gatgcagatg atgggagttc ccgccttgac
    12661 aaaaggaaga aatgcatcta cataatcctg cacggaaggc atggaagact tcggatatac
    12721 atccgggtga tccaccattt cctgatagaa ctcacggaca ccgatctcta tcatttcctt
    12781 ctggtatttc ccctcttcaa aagatacata aaaaggaact accgtaatgt ctttttcttt
    12841 ggtcagctcc atgggaagat cacaggaacc gtctgatata atatgaaatg cttcgctcat
    12901 tctcgcatac acctttctac ttgtttgcag agtttagctt accacgttgt ggagtaaaaa
    12961 gcaatgagtt tttgttatta tatttcattt cgtggtgttt tatgctgttt tatgtagttt
    13021 ttattactat atgtatgaaa aatccgaatt acgcgatatt tgtcgatgaa agtttgtcaa
    13081 ccaacataca acattcgttg acaatgggca gttctctcga tatacttgag ttgttaacca
    13141 ttcattcccc cggttaacat tttctgcagg aatgaccccc tttcattctc tgcaaaccca
    13201 aaaaacagcc cggcaggatc tcctgtcggg ctgcgttatt gtcttctact attcctatct
    13261 ttatatggag taatccagat tctccaggcg cttgttccgt cgttccacca gatccgcaat
    13321 ggtgactccg tccactacct tgttcactgc atcatacaat tcctgccata cctccagagt
    13381 ctcacaggta tcacatcgtt cgcagatatc agcgccttcc tccagacatg ccaccggggc
    13441 cagtgagcct tccgtcagtc gtaagatcat tcctacggta aaatctgccg gatccttggc
    13501 aatacgataa ccgccctggg caccacggac gctttttaca taacctgcct tgttcaaaac
    13561 agcaatgatc tgctccagat acttttcgga aatcccctgt ctggcagcaa tttcttttac
    13621 cttgatacac tctccgttgt tgttcaatgc cagatccagc atgacacgta ctgcatatct
    13681 tcctttggtt gaaattttca tcctatcctc atttccgagc atcttcctgc tctgtttcac
    13741 ttttaatatc agcctgctta ttccaaagca gttcaaaact gctccgatct ttctatggtt
    13801 tccacagatt ctaccttatt ttttctttat ttttcttttt cctatagttc atataggatt
    13861 tgtggaaaca acagaaaaat caggagagca cccatgaatg ctactcgatg gaatgctctc
    13921 cggattttcg cattacttta ctggcacttc accttacttg taagggataa cgccaccatt
    13981 ataattgtca ttgatgaact gcttgatctc atcggacttc agggtatcta ccagtgcctt
    14041 gatcttaggc agattctcgt tgccttcctt aacagcgatt acatttacat aagtagcggc
    14101 tgcttcggaa tcatcgctct catacagcag agcatcgtct gcaacagtca ggcctgcttc
    14161 catagcatag ttgccgttca ggacaccgaa ggatacttcc ggaagggttc tgggaacctg
    14221 tgctgcttcc aactctacga aggtgatgtt gtgagggttc tcaacgatat ccttagtagt
    14281 agcggtcaga cccacaccat ccttcagggt gatgtaaccg ttatcctgta acagtaacag
    14341 agcacgtgcc tcattggtgg tatcattggg tactgcgatg gttgcattgt caatgttgtc
    14401 taagctggtc tcggttccgg gatacagtcc gaagggctcg taatggatac cgccggcatt
    14461 taccagatgg gtacctttct cctcgttgaa gctctccaga taagggatat gctggaagta
    14521 gtttgcatcg aaatcaccgg actctactac taaattaggc tgtacatagt catcaaaaat
    14581 ctgaatctcc agagtataac cctgctccgc taacagaggc tttgctgcct ccaggatctc
    14641 tgcatgaggt actgcacttg cagctacctt gatagtggta tcggtgtcag ctacttcctt
    14701 gctttctgtt gcctcggtag tctgggcagt ggtttcctgg ttagctgttg ttgcagctgc
    14761 ctcgctggtc tgagtagctg tttcgttgct gccacagccg gtcagtacgc tcacggacaa
    14821 tgctgcaact gcaatcaaag cgattacttt ctttttcata atacttctcc tcctaaatct
    14881 tttggtaaaa aattttagtc tattgaatca gatgccttac atctgcctca atcttttcgt
    14941 tttccctaac gggttctctt atccaatttc ttggaaatcg tcattccgat atattgcatc
    15001 agctgtacca gcactatcag caataccaca gtcacccaca tgatgctgct atcgtaacgg
    15061 tagtaaccgt aacggattgc gatatcaccc agtccgccac cgccgacgat acccgccata
    15121 gcggagtatc ccagaatcgt acccagggca atcgttacat tgacgatcag ggaagtcttt
    15181 gcctctccga tcattacttt ccagatgatg gtccagaggt tcgctcccat gctctgtgcc
    15241 gcctcaataa cgccgtgatc cacttccaac agagaggact ctaccattct ggctataaac
    15301 ggtgccgctg acagtgtcaa cggaacgatc gttgcggtcg gtccatagct ttttcctacg
    15361 atgatccgtg tcaacggctg aatcacgatc agcaaaatca aaaacggaaa gcttcgaaca
    15421 acattcacga ccacatctaa taccttataa ataatcttat tcggtcgaag tcccttcggt
    15481 gcggtaatta ccagggcaat tcccatcggc agtccgagca cataccccat aaacgttgtt
    15541 gccaacgtca tgtacaaagt aacaaagatg ccttccacca ccatcatagt cgttgcatta
    15601 tcccacatag tctctcaact cctccacttc cagttttctc tcctgcaaat aatgaaccat
    15661 cttgttcgcc gccatttcat cctccggcag ctgtaagatc atctgtccat gg
//
LOCUS       DAWDHD010000050        15682 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_255_length_15682_cov_13.805529, whole genome shotgun sequence.
ACCESSION   DAWDHD010000050 DAWDHD010000000
VERSION     DAWDHD010000050.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 15682)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 15682)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15682
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_255_length_15682_cov_13.805529"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4765..4864
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4447 a         3148 c         4272 g         3715 t
ORIGIN      
        1 gtagtatccg tgaagacggc ttccgatatt cccaaaggca ggatcgatga ctgcatgaaa
       61 gccctggcag atgtcactgc ggctgcaccc attcatgtag gagatgtcat tgtggaaaac
      121 gtagctgaca cgggtgtcaa tatagtggca acccggtcgt ttcgtgggtg acagaggaca
      181 gaactattga gaatagagca tatttggggc aatggacaca atatattcca atgacacgat
      241 tgggatatgc tatgggcaga gcctggaacg aacaacggga ggttacataa cacaatggga
      301 gatacacata acacacagaa aaagacatac atcatggcac tggatcaggg aaccacgagt
      361 tcccgctgta tcctgtttga taaacaggga aatatctgtt ccatggcaca aaaagaattc
      421 acacagatct atccccagcc aggctgggta gagcataacc ctatggaaat ctggtcctcc
      481 cagctgggag tggccattga aagtatggcg gtgctgggta tcaccgatga ccagatcgag
      541 gctatcggta tcaccaacca gcgggaaacc acgattatct gggataaaaa taccggcgaa
      601 cctgtataca atgctattgt atggcagtgc cgccgtactt ccgatatgat tgaggaactg
      661 aaaaaagacg gctttgatca gatcatccgg gagaagaccg gactgattcc cgatgcctac
      721 ttcagtgcat cgaagatcgc ctggatcctg aataatgtac ccggtgtcag agaacgggca
      781 gagcggggag aattgctttt cggtacggta gatacctggc tgatctggaa tctgaccaag
      841 ggtgctgtac atgtgacgga ttatacgaat gcttcccgga ccatgctttt cgatattcat
      901 aatctttgct gggatcagga gatcctgaag cgcttcaata tcccggagag tctgctgccg
      961 aaggtgtgct gttccagtga gatctacggc aggaccgatg cagccgttct gggcggcgag
     1021 attcctattg ccggtgcggc gggagaccag caggcagcgt tgttcggaca atgctgtttt
     1081 gaacagggag aagtgaagaa tacttacgga accggctgct tcttattaat gaacaccgga
     1141 cacgaagcca ttacctccca gtcggggcta ttgaccacta ttgctgccag cacaagcaag
     1201 aacgtagaat atgccctgga gggcagcgtg ttcgtagccg gtgccgggat ccagtggctt
     1261 cgagacagta tgcgtatgct caagacctcc gcacagtccc aggagtatgc ggagcatgtg
     1321 ccggataccg caggggtcta tattgttccg gcatttgcgg gcatgggagc tccttactgg
     1381 gaccagtatg ccagaggaac catcgtcggc attaccagag gctgtaccaa ggaacatttc
     1441 atccgagcca cgctggaatc tattgcatac cagacggcag atgtgctgga agccatggag
     1501 aaggacagtg gcattgactt aaagagtctg aaagtagacg gcggcgccag tgccaatgat
     1561 ttcctcatgc agtttcaggc agatatcgtc aacacccagg tacgccgtcc ggcatgtatc
     1621 gagacgactg ctctgggagc ggcttatctg gcaggacttg cggtaggcta ttggaagaat
     1681 cgtgatgaga tccgtgagaa ctggcagatc ggagcatcct ttgcaccgga aatggatgaa
     1741 gaagactgca agaaactgtt aaagggctgg catcgtgcgg tgaaatgtgc tcttgcatgg
     1801 gcagaggatg aaatctaaat aaaaccttaa ttttttacgg gtgacgtgta tttttggcgg
     1861 agcaatcttg taaagtgaca aagtactgtg gtatgataac tatttagaat accatggctg
     1921 tttatgatgg cctctgacag gggatgatca atgacagctt ggggattccg gaaaggaacc
     1981 ggcatggatt taaagggttt accacagacc gttcaaaatt tcatagagga aaccatccaa
     2041 aaccgggaaa acggtaaatt tccggacaac gagacctgtc agaaagtgat ggaatatgca
     2101 gcagacaccg gctcacagaa gctggccggc ctggggcttt attatcttgc agaatattat
     2161 tggcagaacg accagtatga aaagaccttg caatgtctga cagagagtat cggttatctg
     2221 aaaaatgagc agatgtatga actgctggcc agaacctata atatgatggg agcggtgtcc
     2281 gatcggaaga acaaccggat ggtggcttta agttcctatt ataactgcct tcaatatgcg
     2341 gaaaaatacc atttttatta tatccagggc atggcggaga gtaatattgc ctataccctg
     2401 gtcaggatgc gcctgcggca ggaggcgata cagcattacc gtacagcaat cgactcctat
     2461 agtaagtcag agaaaacata tcagctgaat tataacagga tcaactgtat gatcgaatgt
     2521 ggctgctgtc acatgtatat gggagaaatg gaagaggctt tacggctttg gaatcaaata
     2581 gaacagatca tcagagaggc accggaaagc tattattcga aaataacatt agaaatgtat
     2641 cgtatttcct gcgaattatt acaagggcat gaggaagagg ctttaaagct tgcagcagat
     2701 cttctggagc aattgtcaga ccgggatatt tttgaagaaa tgttggatga actggtgatc
     2761 ctggcgggaa tcctggcaat cctgcctgat ggaaaatatc tggaagaatt gatccggatc
     2821 attgatgaaa agcatatcga agagcattat aatattttcc tggatctgta tcctttcaag
     2881 agtgaacttc tgcagaagaa gggtctcacc tgggaataca tagattatac ccgccagtat
     2941 tttgatattt atgagaaata ccaacaggaa aacagagaag cgctgatcaa tgtcatagaa
     3001 ttgcagaacc ggctgaaaaa cgttacgttg gactggacga acatgaaagc ctccaaccgg
     3061 gagctggaaa gtctggctat gcatgatgaa ctgacgggac tggcaaaccg gaccttttta
     3121 catgaacact ttacgagcag ttttgaacat gcctatgagg aacatgagct catgggagta
     3181 gaactcatgg atattgattt cttcaaggaa tacaacgatc attacggaca tctggccgga
     3241 gatcagtgcc tgaaagcgat tgccggagtt ctgcggaaac agcaggttcc gggtaagatt
     3301 ttctgtgcca gatacggtgg ggatgaattt atgatcctct atacaggaat gacggtggag
     3361 aagatacgaa gagtggcgga agatattttg agggaggtcc gcaaattgaa acttccccat
     3421 gaatgcagta attgcagcaa ctatgtatcg gtaagccaag gagtatttgt gcggattccg
     3481 gtgggcaata acaaggaatg ggatttcaca tcaagagcgg atgagcttct ctataaggcc
     3541 aaaagctgtg gaagaaacag tatctgtctg tccacggaaa ggttcagaga taagtttata
     3601 gagatcaaat aaacagcgca gaatcaaatt tgaaatttga tttgcgattc ctccgacgaa
     3661 aggaatcgtc tgcggaaagg agattagtat gttagttcag aaatataaag acatgctgtc
     3721 gggaaaatcg gtgatccggc agctttcgga attcgctacg gcaagaggac aggagatcgg
     3781 atacgagaac gtatttgatt acagcctggg gaatccttcc gttccggttc ccagagaatt
     3841 tacagatcgt atgatccata tgctggcaac gaaggaaccg ttggaactgc atggctacag
     3901 tcccagcctc ggtattacaa gtgtcaggga ggcaattgcg gagaacctgg agaaaaggtt
     3961 tgggctgcct tatcgtaagg agcacatttt catggcatcc ggggcggcag gagcgttggc
     4021 acatgctttt cgtctggtga cggagccggg agatgagatc ctgacttttg caccgttttt
     4081 cccggaatat catccttatg tggatctcac cggagcggta ctgaaggtgg ttccccccaa
     4141 tctggagaac ttccagatca acttcgaggc gtttgaggag atgctgacgg agaaggtcaa
     4201 ggcagtactg atcaacactc ccaacaaccc ctccggtgtg gtatattcca ccccgacatt
     4261 gcagagactg gcagatatcc tgagcgaaaa gtccaaggaa tacggtcata ttatttatct
     4321 gatctctgat gaaccttatc gtgagatcgt gtttgaaggt gtagacagtc cctgcgtatc
     4381 ctatttttat gacaatacca tcatgtgcta ttctttctcc aaatctctgt ctctgccggg
     4441 agaacgtatc ggttatgtgg cagtgaaccc ggcatgtgag gatgcggaga ccatgatcaa
     4501 tatgtgcgga cagatctccc gaggtatcgg acataactgt cctccctcca ttatccagct
     4561 ggcagtggca caggtactgg ataagaccgc agatctgtcg gtgtatgaga cgaatatgaa
     4621 tatcctgtat aaagaactga ccgagctggg attcacttgt gtgaagcccg gcggaacctt
     4681 ctacattttc ccgaaagcgc tggaagaaga tgccaaggta ttcagccaga aagcattgaa
     4741 atatgatctc gttctggtac cgggnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnatgcca aggtattcag ccagaaagca ttgaaatatg atctcgttct ggtaccgggt
     4921 gatagctttg gtgcacccgg atatttccgc atggcctact gtgtggacac cgaaaaagtg
     4981 gagagatctc tggaagcttt gcgtaagttc gtaaagaccg agtatgcaac tatctagagt
     5041 cacacagcat atcgaaaagc gaatcgtgct tgcacgaatg agcctttcga tatgctgatg
     5101 tgacgaaata accacatgag caaataacaa gagaacttgt tattcaggaa agcatcgcag
     5161 atgcgatttt gcgaatgtgg ttctggatag ttgcagaagc agaaaggaga gtaatgagaa
     5221 tgtatcaggc aggactggta ttggaaggcg gcggaatgaa gggtgtatac accgccggag
     5281 tgttggactt ttttaccgaa aaaggaattg acttctccca tatctatggg gtatctgccg
     5341 gggcgtgcca tatgtgcagc tatctgtcca gacaaaaggg acgggcgctg agtgtcagtg
     5401 tggattatct ggatacgaga cggtattgca gcctggaaag cttattgacc agcggagatc
     5461 tgttcaatgt ggatttctgt tatcatctga tcccagaata tctgtatcca tatgactatg
     5521 aaacatttga aaaatatcct ggcaaggcct acagcgtggt gaccaacatc gagacgggac
     5581 agccggagta tctgcgtgtc cgtgatatcc gcaaggatat cgacaagatc cgggcatccg
     5641 catccctgcc gctggtggca cggaatgtga agatcgatgg gaagctgtat ctggatggtg
     5701 gcatcagcga tgcgatccct ttggaaaaat cgatcctgga cggcaaccgc aaaaacatcg
     5761 ttgtcatgac caaggaagtg ggattcgtga gaaaacctgc ctctgcatcg cagttaggac
     5821 tcataaaact cagatatctg aaatatccta aagtatatga acttatggcg gacagacata
     5881 tccgatataa taactgcctg gaatatattg acagacagga gaaaaatggg caggcttttg
     5941 tgatccgtcc tcataaaaag agtgatgtgg gacggatcga gaaagacaag gacaaactga
     6001 tcgccttata cgaagagggt tatcgggaag cagaagcctg ctacaaggat ctgctggcat
     6061 atctggaaag gtaagtacta aaaatataag aaagtaaaga gagaagagaa atgttagaaa
     6121 ttatcaaagc aattatttac ggtattgtag aaggtattac cgagtggctg cccatcagca
     6181 gcacgggaca tctgatcctg gtggaacgtc tgatcccttt taagcagacc agtgaaggat
     6241 ttttcgatat gttcgatgta gtcattcagc tgggagctat tttagcggtg gttgtattgt
     6301 tctggaacaa gatctggccc ttttacctga agaaaaataa gcagccgaaa aagggaggca
     6361 ttgtaaaatc cgcaaaagat cccgcaatgg gaaatgtggc actgagcatg gatgctttct
     6421 ggatgtgggt gaagattgtt gtggcatgta tcccggcagt cgtatacggt ctgttatttg
     6481 atgatgcggt gggagaagcc ttccaaaaag agatcggaag cggtggtgtt acaattcagg
     6541 tcatagtggt agctgtaatg ctggtagtgg tcggcgtact gtttatcgtg atcgagaatt
     6601 ggaataagga ccgtgtacct accacaacaa aattgtctca gctgacctac agagatgcac
     6661 tgatcatcgg tctgtgccag ctggtagcgg cggcactgcc cggaacctcc cgctccggag
     6721 ctacgatcct gggagctatt atgattggta tatcgagaac agtggcagcg gaatttacct
     6781 tctttctggc gattcccgtt atgtttggag ccagcctcct gaaggtactg aagttcggat
     6841 ttgcattcac gggaatggaa cttgcctgcc tgctggtcgg tacggtggta tccttcattg
     6901 tatccttgtt tgtacttcgt ttcctgatgg gatatatcaa aaaacatgat tttaaagtct
     6961 tcggatggta tcggatcgta cttggtatta tcgtattact gtacttcctt gtattcgcat
     7021 aacagttgac aattataggg aaaaggaata cactaccctt atcgagcgcc gaaatacggt
     7081 acttgtgagg agaatgaaaa tgtttttgta catgattcgt acagaaggga gacggtaaca
     7141 tggcaatagt taagaaaact gtagcaccag tagcagcagc gaaagtggaa gcgcctaagg
     7201 cagaagtgaa gaaagcagca ccgacaactc cagttaagac agaaactaag accgctgtga
     7261 aggaagctga gaaggcacct gcagcaaagg ctgaagtaaa agcacctgta aaggcagagg
     7321 ctaagaagcc tgcagccaag aaggaaacag ttaagaaaga gaccaagaag gcaacacctg
     7381 caaaggcaga ggctaagaag cccgtagcca agaagacaga gttaaagagt gagatcagcg
     7441 ttcagttcgg tggaaagtcc tactctcagg aagaactgct gaagattgcc aaggacgtat
     7501 ggaaatacga tctgaagcag aaggcagctg acctgaccag cgtagagctg tatgtaaagc
     7561 ccgaagagaa catggtttac tatgtaatga acaaagagat caccggaagc ttctacatat
     7621 aattatccag agtcagataa aatgagcaaa gtatatgtcg aatgcttgca ttcgtaagaa
     7681 tacttgtgag caggccccaa agttgaattt accactttgg ggtcttcgta tctataaaaa
     7741 tgttaaagga tgacagaatg cagaaaaaat cagaaaagat tatttttaca ttatatctgt
     7801 tgggattcct ggcactggca cttacacttg cactgttgca gccgctggcc aacacaccgc
     7861 cgctgtatgg taatccgccg gatgaacatg cgagatatct gatcccgcag tttatctgca
     7921 agtatgggaa gatccctacg ggatgggagg aagaggtgcg gattccggcc tacggatttt
     7981 cctatgctct gtataacgta tttccctata tcgtgcaggg atatctcatg cgctttgtga
     8041 gcctgtttac ggaatcggaa gtggtattgc tctataccgc aagactggtg aacgtaacct
     8101 ttggcctgct gatggcagta gtggtatatt tgattggaaa aagagtgttc cgggatgacc
     8161 gattccgttg gctgttctgc tttgcagtaa cgtacctgcc ggagggattg ttcctgcaca
     8221 cttatgtgaa tacggattcc tgctgtatgt tatccacggc catgatggta tatgctctgg
     8281 tatgcgtata tcaggatggt ataagtctcc gcaacagtct gtggatgagc ggtggtatta
     8341 ttctgtgtgc tctttcttat tataatgcct acggatatat cgtaagctgc attttactct
     8401 ttctgttgtc ctttttgcag aagaaggaaa acggcggcta tttctatgac tggaagaata
     8461 tgctgaaata cggctgtctg atcgccggag tagttctggc ggggatcggc tggtggttca
     8521 tccgcagtta tatcgttctg gatggagatc tgctgggact ggccacccgg gaaaagatgg
     8581 cgatccaata tgcggtggag agtgtcaatc cgctgaccat gcagacttat cagtccatgg
     8641 gttatacggt gctggaaatg ttccgggaac gctacacact gtccggtctg ttccacagct
     8701 tcgtggcagc tttcggctcc atgtccatct acggaagcat ctggctgtat cgggcgtata
     8761 aagcattttt tgtgcttggc accgcgggaa gccttctgta tgtgatctgc tataagaagc
     8821 gtcgcaaaat atccggaaga gaatggttct tccatattaa tatgctttat tgcattttta
     8881 tgccggcatt tctgacgatt tattatgcat acaccacgga ttatcagaat cagggacggt
     8941 acctgctccc agcgctgctc cccctgatgt attatatgat aaaaggaatt caaaaacttt
     9001 cggaactgaa gatcaaaggc agaacctttc cccggtggct ggtgaataca ggggttgcga
     9061 tatgttttct gatccttgtc ggaggcacaa tagatatggt atatgtacgg gcacttcccg
     9121 tatatctgga gacaggactg gtactgaaat agtgccggtc ctttttgtgt atacgatgag
     9181 ccaagtacag atttgtgctt gcacaagaat ctgtatttgg cgaatgaggc tctgaatagt
     9241 tttttttaat attttgttta gaattatttt cgggaaaatc agttgacaat aatatcctgt
     9301 agtgatattt tagggtaaga agatgttagc actcctttta gatgagtgct aataatccca
     9361 aagggagtga tggaatgcaa ttggatgaca gaaaaacaat tatattgcaa gccattatcc
     9421 ggaactactt ggaaacggga gagccggtag gcagcaggac catttcaaaa tacacagatc
     9481 tgaatttaag ctcggcgacc attcgtaatg agatggcgga tctggaagag atgggttata
     9541 tcatacagcc gcacacttcc gcaggaagaa ttccttccga taagggatat cgcttctatg
     9601 tggataccat gatggccacc agagagcagg aagtcaatga gatgaaggat atgctcttag
     9661 agaggcagga taagctggag aacctgttaa aacaggtggt gaagaccctg gctcagaaca
     9721 cacaatacgc caccatgatc agtgctccgc aggtacatcg caacaaggta aagttcatcc
     9781 agctgtcccg ggtagatgcc ggacagattc tggcggtcat cgttgccgaa ggtaatgtga
     9841 tcaagaacaa tatcctggcg gtgaatcagg aactggatga tgagacactg ctgaagctga
     9901 acattctgtt gaatacccac ctcaatggat tgtccatgga ggagatcaat ctgggaatga
     9961 tcgcagcaat gaagcagcag gccggaatcc acagtgagat cgtcagtgag gtgatcgatg
    10021 ctgtggcgga tgccatcaag gcagacgagg acctggagat ctacaccagc ggtgccaaca
    10081 atattttccg gtatccggag ctggcagatc agtccaaggc aagtgaactg atcaatgctt
    10141 tcgaggagaa gcagcagtta ggcggtctga tccaggaatc cctggcagac gagggcagca
    10201 caggtatcca ggtgtacatc ggagacgaga caccggtgca gggcatgaag gactgcagtg
    10261 tcgtgaccgc tacttatgaa ttaggcgaag gcatgaaggg aaccatagga atcataggtc
    10321 ccaagagaat ggattatgac aaggtcatag gaacgctgcg gacggtgcag aaccagttgg
    10381 atgaccttta ccgcaaagaa tagacagatt ccagaaagga aagtaagaat ggcagatcag
    10441 gaaaaagaaa tattagagga agaaatggaa caggatcttt cagatgccga agaactgaag
    10501 gaagaagaga cagaagatga ggaacccgtg agcaaggaag acaaaaaggc agcaaagaag
    10561 caggcaaagc tgaacaaaaa ggaagattcc tacaaggaga agatcgatca gctggaagac
    10621 agagtaaagc gtcagatggc agagttcgag aacttccgca agagaaccga taaagagaaa
    10681 caggcaatgt tcgataccgg tgccaagagt gtgatcgaga agatccttcc ggtagtagat
    10741 aattttgaaa gaggcctggc aacggttccc gaggaatcca aagaggatcc tttcgtagac
    10801 ggtatgaacc gtatctacaa acagctgatg acagaacttg agaacatcgg tgtaaaaccc
    10861 atcgaggcag tcggacagga attcgacccg aatcttcaca acgcagtgat gcaggtggag
    10921 agcgatgagt atgaatccgg tgtgatagcc caggaacttc agaagggtta cacatatcat
    10981 gacatggtcg taagacacag catggtaggc gttgttcaat aattgattat cacatatttt
    11041 tgagaataga aactataaaa tgaaaacaga gaataaaaac aggaggaaaa tattatgagt
    11101 aagattattg gtattgactt aggtacaacc aacagctgtg tagctgttat ggaaggtggt
    11161 aaacccaccg ttatcgctaa tacagaaggt gcaagaacca caccctctgt tgtagcattt
    11221 accaagaccg gtgagagact ggtcggtgag cctgcaaagc gtcaggcagt taccaacgca
    11281 gagaagacca tttcttccat taagagagat atgggtaccg atcgcggccg taccattgac
    11341 ggcaaaaaat actctcctca gcagatctcc gcaatgatcc tgcagaagct gaaagcagat
    11401 gccgagagct atctgggtga gaaggttacc gaggcagtta tcacagttcc cgcatacttc
    11461 aatgatgccc agcgtcaggc taccaaggat gcaggtaaga tcgcaggcct ggatgtaaag
    11521 cgtatcatca acgagcctac cgcagcagca cttgcttatg gtctggacaa tgaaaaagaa
    11581 cagaagatca tggtatacga ccttggtggc ggtacctttg atgtatctat catcgagatc
    11641 ggtgacggtg tcatcgaagt actggctacc aacggtgata cacaccttgg cggtgatgat
    11701 ttcgataaca agatcattca gtggatgctg gatgaattca agaaggcaga aggcgtagat
    11761 ctgtccggtg ataagatggc tatgcagaga ctgaaggaag cagctgagaa ggctaagaaa
    11821 gagctgtcca gcgcaatgac caccaatatc aaccttcctt tcattaccgc aactgcagag
    11881 ggacctaagc actttgatat gaacctgact cgtgcaaagt tcgatgaact gaccagagac
    11941 ttggttgata agactgctat ccccgtacag aacgctatga aggatgctgg tctgaactat
    12001 tctgatctgg gtcaggtact gctggtaggt ggttctacac gtatccctgc ggtacaggat
    12061 aaggtaagag ccctgaccgg taaagagcct agcaaatctc tgaaccctga tgagtgcgta
    12121 gcaatcggtg cttctatcca gggtggtaag cttgcaggtg atgctggtgc cggcgatatc
    12181 ctgttactgg atgtaacgcc cctgtccctg tccatcgaga ccatgggtgg tgtcgctacc
    12241 agactgattg agagaaatac aaccattcct accaagaaga gccagatctt ctctaccgct
    12301 gctgataatc agaccgcagt agatatcaac gtagtacagg gtgagcgtca gttcgccaaa
    12361 gataataagt ccctcggaca gttccgtctg gacggtattg ctccagctcc cagaggaatc
    12421 cctcagatcg aggttacctt cgatatcgat gccaacggta ttgtaaatgt atccgctaag
    12481 gatctgggta ccggcaagga gcagcacatc accattaccg caggctctaa catgtccgat
    12541 tccgatatcg agaaggctgt caaggaagca gctgaattcg aagcacagga taagaagcgt
    12601 aaggaagcta tcgaggcaag aaacgaagca gactctttcg tattccagac cgagaaggct
    12661 ctgaatgaag taggtgacaa ggtacccgag agtgacaaga ctcaggtaca ggctgatctg
    12721 gaagctgtga aggctattct ggagagaacc aaagaccagg agatgagcga cagcgatgta
    12781 gatgagttga aggctgcaaa agagaaactg acccagagtg ctcagtctgt attcaccaag
    12841 atgtatgagc aggctgctca ggcacagcag ggtgcacagg gtgcagcagg ccctgacatg
    12901 aatgcacagg ccggtagcaa tgctggatct aacgcagacg atgatgtgat cgacggagat
    12961 ttcagagaag tataattaaa cttcacaaat aacgcaaatc gtggtataac agtaaatgct
    13021 gtgtaacagt gattgcgaat aacaggtggc acagggtatg cgaaagcgtg ccctgtgcat
    13081 aaccgttttt agatttaaat gacagaggtg caaaaatggc agagcagaag agagactatt
    13141 acgaggtcct cggtgtagat aagaatgcag atgatgctgc aattaaaaaa gcataccggg
    13201 tactggcgaa aaaataccat cccgacatga accccggaga caaggaggca gaacagaaat
    13261 ttaaagaagc ttccgaggct tatgccgtgc tgagtgatcc cgagaagaga cgtcagtatg
    13321 atcagtatgg ccatgcggca tttgacggtg gcgcaggagg tgccggtggt ttcgatttca
    13381 atggagctga ttttggagac attttcggag atattttcgg agatatcttt ggcggcggca
    13441 gaagcagagg tgccagaaat aacggcccca tgaaaggtgc caacatccgt accagtgttc
    13501 atatcacttt tgaagaggca gttttcggct gcaagaaaga gatcgatctt acggtaaagg
    13561 aaacctgtaa gacctgtaac ggctccggtg cgaagccagg aaccagccct gagacctgta
    13621 ccaagtgtgg tggtaagggt caggtggtat ttacccagca gtctttcttc ggtacggtac
    13681 gtaatgtaca gacctgtccc gactgtcagg gtaccggtaa ggtgatcaag gacaaatgcc
    13741 cggattgccg tggtaccgga tatattccta tgaagaaacg ttatgcggtg gatattcccg
    13801 caggtatcga caacggacag tctacccgta tgccgggact gggtgagcca ggtgtcaacg
    13861 gtggccccag aggcgatgtg ctggtagaag tgatcgtagg tcgtcatcct atcttccaga
    13921 gacaggattt cgatattttc tctaccgtac ctatttcttt tgcagtagcg gcattgggcg
    13981 gcgaagtgct gattgatacg gtagacggca aggtggtata tgatgtaaag gccggaaccc
    14041 agactgacac caagatccgt ctgcgcggca agggtgttcc ttcctggaga aataaggatg
    14101 tccgcggtga ccattatgta acactggtgg ttcagactcc cgacaagttg aaaccggaag
    14161 caaaggaact gctaaagcag ttcgatgtac tgaccggaga ttccctgaat gttgtgaaga
    14221 aagcaacgga aagcggagag ggagagacca gggagaatga ctccagggac ggcaagaaga
    14281 aaaagttttg gaagtaaaag ttaataaata aaattccccg tagcctcggc tggggtaaga
    14341 aacgcggcac gctcgcgctc tatcgcagag cgtagcggca ctgcttattc ttacacccag
    14401 ccggggctac tggtttgttg ggaaaaagtg aaaggtatct ttgaaggcca atgatataga
    14461 atacagtttg gtttttttgt caaaaggcag ggtgaacggt ttgacagaat gggcagaaac
    14521 gcttacaatt caagtagtta tgctgtctgt gacacgaagg agcctgcagg atggagaacc
    14581 ggaaagagga atttctgaaa attgtatgtc agagttatct gatcgttatt ctggcagtgc
    14641 tgcctcttta ttatattccc tggaatggat attataaact gggtgatacc aaatattacc
    14701 tgtatcgcaa tgtttcactg ctttgccagg ggatcgcatt gctggcactg tgtgtctttg
    14761 ctgtaagcag ccggtggaca ggggaacatc ggatttttgc cagatctctg gctgaagtag
    14821 tgaaaaaatc ggtcgataaa tgcagaacac atgcggtaac gacggcggtc tgcctgtacg
    14881 gaatctgtgc gttactctct gcaatctgca gcccctatgg cagtattgcg tggaacggag
    14941 agcgggaatg gtacatggga gctgtcacga tctgtttgat gatcggaggt tttttactga
    15001 cggcgaagta cggaggtagt tgtaaaacgg cgatatggtt aggagaggcg gcttttgtgg
    15061 cggtcaccct gatcggtctg ttgcagaagc tgggctatga tcctctggga cttctcaagg
    15121 gttacgttgt gggggactgg gaatttaccc atatgttgac cacgctgggg aacagcaact
    15181 ggctcagcgg gtattacagt gtgatgtttc ccttttccat gacgctgttt caccgggcag
    15241 tggaagcagg gaaaaaaggt cccacactgc tggcaggaac gtgcaatatg ttggccatga
    15301 tgcttctgct tttacaggga agtgacagcg gtgtgatcgt ggcatgcaca gtcatggggg
    15361 tgtgcttttg gctcgaccgg aagcaaaccg gacattggga agtatatttc ctttttcttg
    15421 cagcggtggc gctgggaatg tgccgttggg gtaaatggat gttatatctg ggaacgtttg
    15481 acattctcct gcaggatggc atcgccaaaa aactggcagc ctggcaggga tggattccgg
    15541 ttgcagcagg gtgtctgctg ctttggggag cctgtcgcag gattccggag aataagaaaa
    15601 aaatattgca agagggagta ttgtgttgtg cactggcagg aatagcggta gggctggctt
    15661 ggtatctgcg gcgggtgggg gg
//
LOCUS       DAWDHD010000051        15470 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_259_length_15470_cov_11.722802, whole genome shotgun sequence.
ACCESSION   DAWDHD010000051 DAWDHD010000000
VERSION     DAWDHD010000051.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 15470)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 15470)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15470
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_259_length_15470_cov_11.722802"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         4477 a         3286 c         4249 g         3458 t
ORIGIN      
        1 tgggcaaaac tccgacttgt gaaagaggtg gcggaggaag tctggggaaa ataaataaac
       61 gtggttccgg cgattaccag agaggtggtc gccggaattt taattatatt caattgtctc
      121 atttttctgg ataggctata atatacctat ggttatccct gtttaacatt ttctaaatgg
      181 ggatttttga aatattttga atatgaaaaa taatacggag gaagcagaaa catgaataaa
      241 caaacagccc attaccatct tcccggtctg tttgaattct atgaattata cagaatgttc
      301 ctgccgttgt ttcgggagca tcgagaatat ttttacgact ggtgcgacat cggttccatc
      361 tacggggccc cgccggactg catctgggga ggcggccggg tctcactgga ggatcacgac
      421 gcaggagaag tgctggcact gttacaggaa tacgggatct cagcccgtct gaccttcagc
      481 aattcaaagc ttatcgaaga acatctttcc gacagaaaat gcaatgaact ctgtgccctg
      541 tttgcggaaa atgcaacccc ggagaacggc gtgatcgtcc actcggagct gctgttacag
      601 tatctgaaaa gtcattatcc ggagctttat ccggtatcct ccaccacaaa agtcctgacg
      661 gattttgaag cgttaaaaaa ggagaccgac agagacgatt ttcgctatgt tgtaccggat
      721 ttccgcctga acaaggctta tgaaaaatta aatatgctga cagaaccaca gaaagaaaaa
      781 gtggaatttt tgtgcaatga atgctgctat tttggctgta aggacaggaa ggaatgttac
      841 gaagccgtca gccgcaggaa tctgggagag gagccggatt ttagctgtac ttcgccgggg
      901 gcggaggagg gctatcgttt ctccaaagcc atgaaaaatc cgggatttat cagcgtggaa
      961 gatattcaga agatctactt gcccatggga ttttccaatt ataagatcga gggccgggga
     1021 ctgggcagtg cactggttct ggaattttta ctgtattata tgacgaaacc ggagtaccag
     1081 ctgcaggtca gagaagaaat ctatctggac aatatgctgg atctgtttta ataaatgaaa
     1141 tcaaaagatg aggaatgctg tcatggaata cgaaaccaat gaaccaaagc atatcaaggt
     1201 aagaggtgca aaggttcata acctgaaaaa tatagatgtg gatgttcctt tacacaaaat
     1261 cgtggggatc gccggggtat ccggttcggg gaaatcttcg ctggcgttgg gtgtcctcta
     1321 tgcggagggc tccagacgtt atctggaatc cctgtccacg tatacgagaa gacgcatgac
     1381 aggtgcggcg aaagcacagg tagatgaagt actctatgtt cctgcggcgc tggcattgca
     1441 tcagcgtccc ggcatcccgg gaatccgcag taccttcgga accggcacgg agcttttgaa
     1501 cagtctgcgc ctgatgtatt ccagactggc atcccaccgc tgccctaatg gacattatgt
     1561 gccgccgacc ctgaaagtag cggcggagca ggagatcctg tgtcccgaat gcggagcaaa
     1621 ggtgcatgct cccagtgcag aagaactggc attcaattcc cagggagcct gtccgaagtg
     1681 cggtggtacg ggaagcgtgc ggaccgtgga tctggattct ctggtgccgg atgattccct
     1741 aagcattgat gagggagcgg tagcaccctg gaacagcctc atgtggtcat tgatgacgga
     1801 tgtctgccgg gaaatgggag tgcggacaga tgtacccttc cgggaactga cagaccggga
     1861 aaaagagatc gtttatcacg gacccgcgga gaagaaacat attttctata aggcgaagaa
     1921 gaccaatcag gcgggagaac tggactttac gtattttaat gcggtataca cggtagagaa
     1981 tgctctggca aaggtaaaag atgaaaaggg catgaaacgt gtggaaaaat tcttaaagga
     2041 ggatatctgc ccggaatgcg gcggcagcag actttcggaa gctgccagag caccgagact
     2101 tagggggata gggctggcac aggcctgcca gatgcccctg aaagaattgg tggactgggt
     2161 agcgggagtg ccggattctc tgccggaaga gatgcacccc atggcggaat ccatctgtga
     2221 atctttccag actgtggcga aaaggctgat ggatctggga ctttcttatt tgtctctgga
     2281 ccgtgcggca gcctccctgt ccactgggga aagacagcgg atgcagctgg cccgggcggt
     2341 gcgaaaccgt accaccgggg tactctatgt gctggacgag ccctccatcg gtctgcatcc
     2401 ttcgaatatc gtgggactga atgccgtgat gcatgatcta attgctgatg gcaattccat
     2461 cattttggta gatcatgata cccagatatt atccgaagcg gactggctga tcgagatggg
     2521 accggaagcc ggtgccggag gcggctatgt gatcacacag ggtacgattc cacaggtaat
     2581 cgcaaaaaaa gagtccatga tcggtccttt tctggcgcag actgcggata tgcgtatccg
     2641 taagcccatc acccgggagg aaatatttgc accgggaaag cttcatttgt ccacagatgc
     2701 catccatacg gtaaaacctc tggaaataga tattcccaaa ggaagactta cggtcgttac
     2761 cggtgtctcc ggctccggga agaccaccat ggtgctggaa agcctgattc ccggactgga
     2821 agcaaagtta aacggcgaac atttgcccgg ccatgtaaaa agtatcaccg cagagggcat
     2881 cgcccatgta aaactgatcg atgcatcccc catcggcatc aatgtgcggt ccacggtagc
     2941 cacttatgcc aatgtgcatg acgaactgcg gaaaatttat gcaaaaaccg cagatgcgaa
     3001 aaataagaaa tataaggcgg gagatttttc ctacaatacc ggaaaattaa gatgcccggt
     3061 ctgcgacggt accggacaga tcagtctgga cgtgcagttc ctgccggatg tggatgtgcc
     3121 ctgcccggag tgtggcggtt cccgttatgc cagagaagcc tgggagattt gttatgagaa
     3181 caaacagggg atgcgttatt ccctgcccca gatgatggag atggatgtca cgacggcgct
     3241 gcaggcaaca caagaatgga aagtggtcca tcagcggctg gaagtcctga aaaatctggg
     3301 actgggatat ttaacattag gcgaagagac accaagcctc tccggtggag aggcccagcg
     3361 gctgaagctg gccagtgaaa tgggcagagg acaatcagat tccgtatttg tattcgacga
     3421 accgacgatc ggactgcatc cgttagatgt acagaccctg ttgcaggtat ttcagacgct
     3481 ggtagacaat ggggcaacgg tacttgtcat tgaacatgat ctggatgtga tccgcaatgc
     3541 cgattacatc attgatatgg gacccggtgg cggagaagac ggcggcagag tcgttgctgt
     3601 gggaactctg gagcagatca aggcagatgc cgacagcatt acgggaaaat atctgtaaaa
     3661 aatcaaaaac ccagatacgg cagaggcatc cgctcaccgt acccgggttt taaaaaactg
     3721 atttttacag aatcacctgc tccagcttct cacggaagtc tgcttcggac atggcgccga
     3781 cgaagttggc cacgggttgt ccgtctctga agatcacaaa attcggaatg cttgccacgc
     3841 ggtacttctg ggcgatggtc atgttctcat ccacattgat cttgccgacc tttacttttc
     3901 cctcgtaagc ttctgccagc tttgccacgg taggagccat catcttgcag ggattgcacc
     3961 agtctgcgta gaaatctacc agaacgggca gaggggagtt cagaacttct tcctcaaaat
     4021 tagcaacggt aaacgtatat tccataagta tccttccttt ctgttatctg ttgttgatag
     4081 tatctcaaaa ataagattct atattcgggg ccttatctgt aaataatata tttgcagatg
     4141 ggattgccaa cggaggaaaa cttttcctcg tattcggtca tgacgttccc ctccatcagc
     4201 gcggcatcgt tatgcagatc gtaggtgatc acctgcgctt tccatccggc gggctccagt
     4261 tcctctactg caaaatcaaa caacgggcgg ttgtctgtct tgaattccag cgtaccctct
     4321 tttttcagga tcacatcata gcgggccaga aactgtctcg aaggaagtct tctcttcgca
     4381 tgacggtcct tgggccaggg atcggagaaa ttcagataaa tgcggtccac ttcacctttt
     4441 ccgaacacct tatcgatgtc ttctgcatcc atacggatga atttcaggtt gggaagctcg
     4501 tcctcttcca ttttctggat cgcgcggagc aatacgctgg aatatttttc aattcccaca
     4561 taattgatct gcggattctg ctttgcaagg atatgcagga aacgtccctt tcccatacca
     4621 atctcaatat ggatcggatg atcgtttccg aaaatctcat gccaggttcc ggggcatgct
     4681 tccagggtat cttccggtac gacataggga ctttccgcaa taacttcccg ggaaccggta
     4741 atatttcgta acctcatggt aatctcagcc tttcttatag taatctgtat tgtcaatgta
     4801 ctttatctga gaaagcttgt caagtcacag gccgggggat tatactggca gtaggaggag
     4861 aaaatttatg aaatggatga ttgcatcgga tattcacgga tcggcatatt actgtcgaaa
     4921 actgttggaa gcatatatgg aggaaggggc cgacaggctg cttttactgg gggatatttt
     4981 ataccacggc cccagaaatg atctgcccag agattatgca cccaaggaag tgatcgcttt
     5041 attgaacgag cggaaaaatg atatctactg tgtacgcggt aattgcgata cggaggtgga
     5101 tcagatggtt ctggaatttc cggtgctgag tgattattgt gtacttgcgg aagggacgca
     5161 ggcaatctat gccacccatg gacatatcta taatgagcag aaactgccgc cattgcattc
     5221 cggagatatt ttattgcacg gtcatactca tgtgccgaaa tgcgttgttc atgagacaga
     5281 gcagtatatc tgcatgaatc cggggtccgt gtcgattccc aaggaagaaa gctggcacgg
     5341 atacatgatc tggaaaggca aaaattttgt gtggaaagac ttcgacggac aggaacattt
     5401 acggtattcc gtatagagaa tgacagcgtt cgcagaaggt cttaaaaaga attaaaaaat
     5461 agagataaga ctttggtttt taggtaaaat ggtgtatgat aaagaatgcg cattatcata
     5521 caccatttta ttttgaagat atcaggaagg atttatacat gcagtttttc aggcattttt
     5581 catatagaag ttttttaacc agggcagtga tgggaatcag cgctctctgc ctttttgcat
     5641 ctcaggggct tagtgtctct gctgcaacga tcaaagaaga gaacatcgcc cacaatcagg
     5701 cactggccat tcagtccaat gaagtggcaa actggcctac gggaccggta gtcagcgcag
     5761 aatccgccat tttgatggat gcggataccg gagcgatcct ttatgccaaa aatatccatc
     5821 agcaggaata ccctgccagt accaccaaaa ttctgacaac attgatcgca tccgagcgct
     5881 gcagcatgga tgagatcgtg gatttctcct atgatgcggt acacgatatt gatcccggaa
     5941 gcaaccatat cgctattgac cccggcgagc agctgaccat ggaggaatgt ctgaatgcca
     6001 ttctgatccg ctccgccaat gaagtttctt tcgcggtagc agagcatatt tccggaacga
     6061 cctggcagga ctttgcaccg atcatgaatg aaagagccaa ggaactgggg tgtgttgatt
     6121 ccaatttcgt caatcccaac ggacttccca atgaggatca ttacaccagt gcctatgatc
     6181 tggcgatgat cggaagagct ttttttgcca acgaagcgct gtgtaaaatg acaatgacac
     6241 atatgctgca cattttgccg tcggaacgcc agccagatga tattatggaa gtcaacaaga
     6301 tggaactgat ccccggcggt aaatatgcat atccgtatct ggtaggctgt aagaccggtt
     6361 acaccgatgt tgcccgcagt acactggtgt cctgtgcgga aaaagacggc atgaagctga
     6421 tctgtgtggt catgaaagac gaaaatccga attattatga agatacaatc actctgtttg
     6481 attatggttt cagcaatttt cagagagtga acatttctca gacagagacc aagtataata
     6541 tcgagaatgt aggatctttt tacagtggga atgatatttt cggcaactcc aaaccgattc
     6601 tggaactgaa ccagacggac agcatcactt tgccgaacac catcaccttt caggatgcgg
     6661 tgtccagtat ttcctatgat aatacagaac caggacaggt ggctgtgatc acttatactt
     6721 acaatgatgt ggtactggga acggcatccc tggactttac ggcagcggaa aagggcagta
     6781 gtgtcttccg ggaaaatacg gcagagacca cggagtctgc agaggaaagc ggtgcaaccg
     6841 atgaaacgca gtcaggcaca acgaccacag aggcagacaa tacagagaca accgaaacag
     6901 caggagataa caccggaaac aatggtggaa ttccaacggt tgcacaggag acatccccgg
     6961 aggaggaaaa cagcacttcc ggcggtcctg cggttatttt cattaatgtg gtaaaagtat
     7021 ttttctgtat cgtgggaatt gcggtattga tcttccttgc actgttatta cgtacgtttt
     7081 tcaagaatta tcattttgcc caccgcagta cccgcctgac ctggagattt aaccgcagaa
     7141 gaaatcgttc cagatcccgg tatcgttcgg tggatcagga ccttcgtaac cgacgcaaag
     7201 aagcaatccg acaggcgaaa aaacgtcaaa agcccaagaa gtcatcccac tttcgtgggt
     7261 agtattccga aggggagatt tccacacatt ttgcccagtt tgattttgac atatcaatgg
     7321 gtactatggt acactgaata agaaaaaagt ggggtagtgt acccggggaa atgagaagct
     7381 taggataaga aaaggttaag ggagcgaaat accttggata agagtgaata taaattaaga
     7441 gcggaagaaa tcaaagactt gatcagccgt ggggagtatg cgcaggctgc ggagattgcg
     7501 gataccattg actggcgcag agtcaaaagt gttatgatgc tgtgcactat cagtgacctt
     7561 tacaagatca atcgaagata cgaggatgcc agagatatgc tgcttctggc atatgaacgc
     7621 cgcccgggag gaaggacgat ctgctattct ctgtgcgaac tttccattaa aatggaagag
     7681 tatgtacagg ctatcgagta ttacaaagag tttgtgcagg tagcgccaaa ggatcccggc
     7741 agatatattt tgcagtacaa attatatgag gcgcaggatg tcagtctgga agagcgcatt
     7801 gctgttctgg aagaactgaa aaagagagat tacagagaaa aatgggcata tgagttggct
     7861 tatctatatc acagagtagg acttgctgcc agatgtgtag aggaatgtga tgagctgatc
     7921 ctttggtttg gcgagggcaa atacgttatc aaggcaatgg agctgaaaat gctccatcag
     7981 ccgctaacac cggaacagca ggaaaaatac gatcatcgtt ttgacgcgcc cggtacgcag
     8041 cagtattctc agaattatgc gcaggatccc ggttatggac agaacgaaac ttatgatccg
     8101 aatcagggct atgtgcaaga cggaagttac gacccgaatc agggctatgc acagggcgga
     8161 agttatgacc cgaaccaggg ctatgcacag gatggaagtt atgacccgaa tcagggttat
     8221 gtacaggacg gaggctatga gccgaatcag ggttatgtac aggaggaaac ctacgagcag
     8281 gtgaccggtg acaccagagg ctatgaaacg gtgcagccgg tacagcccca gcaggcaccc
     8341 gcgcagccgg cggaagatga tttcgacatt catgtgaaga ccatggatgt gggacaatat
     8401 aatacgatca atctacaggc agaactggca gcaggcctgc gggaggtact gagtgaggac
     8461 catgcgaagg agacttcgga ttccattacc cgttctatcg tggcacccat gatcgatccc
     8521 ggggattccg atacggagtc tctggactat ccggagatcg cagatgtcag tgaagacgat
     8581 ctggagccgg agacggagca gatggagagc tcggaagtat tctttgggga gactggagaa
     8641 atcggcgatc tgtcacaggt gccccaggta gagacggaag agattctgcc ggaagagcct
     8701 gttgttgcaa gaagaaccga tgtagtaccg gagctatccg aagtacagaa gacaccggaa
     8761 gtacaggaaa tcgtgaagga accggaagca cagaaagtac cggaagcttc ccgaacgcag
     8821 gagattcatg aagttacggc atcggcgccg gcagctcctg tagcaccggt aactccggca
     8881 gctcccgtag agccgcccaa ggaactggcc gatgtgctgg cacaggaatc cgacggtcag
     8941 atcagcctgg taatgccgga ggcagagagc attgagaaac agatcaccgg ccagatgaat
     9001 atcgaggata ttctggcaga gtgggaacgc aagaagaagg agaacctgga gaagagagaa
     9061 gaagaggttc gccaacatgt attacagcag accggagcca tgttcacgga gtttgaacag
     9121 gcggtaagag acggattgct ggaaaaactg gaaaaagaaa aagctgcgga cacggcagat
     9181 accgtggcag aagatacaac agataccgat gaggtagaag agctggaaga gatcacggaa
     9241 gagcctgcca cagaggaacc cgtggaagaa ctgacggagg ctgctcctgc agaggaagcg
     9301 gaacaagagc ctgtagaaga gcttccggaa gaggatactg tagaagaact cgcggaagaa
     9361 cctgcatatg agccggaagc agaagaattt gaggaacctg cggaaccgga agcagaaatc
     9421 tatgagcagg aagaaatcgt agacgaggaa caggcagaac cggaaggagc aatggatgac
     9481 gagcctgtag aggagcccac ggcggaagca gtagaatcgg tagatgagga aacagccagg
     9541 gaagagatag agctgacggc tgaggaaacg ccggaagccg gggaagcacc ggaacagccg
     9601 gagcgccccg cagtagagcg agacaaagca aaggttcgtg cgcttaccag agaagagaga
     9661 gaactgtacg caccctttat ccagagcaga tctgccagag agcagttagt caaggccatt
     9721 gataatatca gtctggcagc ctacaccggc aacgtgatca tcactggaga agagggtatg
     9781 gataccttat ctctggcgaa gaacatggtt cgcgaaattc aggcaaccga cagcaatttc
     9841 tccggaaaag tggcaaagat ctctggacat gcattgaata aaaaggatac tgcagatacc
     9901 ttatccaagc tgaaaaacgg cgcgctgatc atttgcaagg catccgaaat gaacgatgct
     9961 acggcaaatg tacttcacaa ggcattgcag caggagagtc agggaatcgt aattattctg
    10021 gaagatacga agagggatat tgataaattc ctcgagaaac atgaaaaatt gagagagtgc
    10081 tttaccgcac gaatggatgt ggaagccctc agcaataaca cgctggtggc attcggcaaa
    10141 cagtatgcac gggagatgga atactctatt gacgaactcg gagagctggc gttacatacc
    10201 agaatcgaag atatgcagac gatagatcat gtggttacgg tagtggatgt aaagcagatc
    10261 gtggatgaag cgatcgctca tgcaaataaa aagacattga agcatttctt cgatgtactg
    10321 ttcgccaaga gatatgacga tgaggatatg atcatcctca ccgagaaaga ttttgtataa
    10381 cttattctga acagttaaat tttatagcgg gaggcgcggc catgacgaag gagaaggtat
    10441 ttatttccga agcagatcag tatctttttg cccaggggac ccattatgat atttacaaaa
    10501 aactgggtgc acatctgtcg gtggaagacg gcgtgcaggg agtctatttc ggagtatggg
    10561 caccaaatgc agcacaggtg aatgtcattg gcacattcaa tgaatggaat gaggaatccc
    10621 atcccatgca gaagctggga gaaggcggta tctgggggct gttcattccc ggcgtggaag
    10681 agggaacgat gtataagttc ctgatctatg caagggatgg cagaaagctc tacaaagccg
    10741 atccttttgc caattatgcg gagtaccggc cgggcactgc atctatcgtt accgatatta
    10801 caggctttga ctggagagat tccaagtgga tggaagcccg ggacaaaaag gatatgaata
    10861 aagagcctat ggcgatctac gaatgccata tcggttcttt tatgaagcat cctaacaacg
    10921 gtaccgccga aggattctac aattaccggg agtttgcgga ccggatcata gaatatctga
    10981 aggagatgaa gtatacccat gtagaactga tgggtattgc agagcatccg tttgacggtt
    11041 cctggggata tcaggtgacc ggctactatg caccgacttc ccgttacgga acgccgaaag
    11101 atttcatgta cctgatcaac gaactgcaca aggccggagt cggcgtgatt ctggactggg
    11161 tacctgcgca ttttgcaact gatgcccatg gcttggctga atttgacggt cagtgcattt
    11221 ttgaacatcc cgatccccgt ctgggagagc atcccgaatg gggaaccaaa atcttcaatt
    11281 acggcaaaaa tgaagtaaag aacttcctgg tagccaatgc cttgttctgg ctcagagagt
    11341 tccatgtgga cggcatccgt gtagatgcgg tggcatccat gctatatctg gattatggta
    11401 aaaaagaagg tgagtggctg ccgaataaat tcggtggcaa caagaatctg gaggccattg
    11461 agttcttcca ccatctgaat tctgtcatcc gtggaacgta tcccggattt gtgacaattg
    11521 ccgaggaatc cacggcgtgg ccgaatgtga ccagtgatat cggaggcgag ggattaggct
    11581 tctcctttaa atggaatatg ggctggatgc atgatttctg tgaatacatg aagctggatc
    11641 ctttgtatcg taagggcaat cattacgcta tgacctttgc catgagctac aatgacagtg
    11701 agaactacat tctgcccctg tctcatgacg aagtggtaca tctgaagtgt tccatggtaa
    11761 ataagatgcc aggttatact gcggataaat acgcaaatct gcgtgtgggc tatacctaca
    11821 tgttcggaca ttccggcaaa aagttgctgt tcatgggaca ggatttcgga caggagcgtg
    11881 agtggagtga agagcgcgaa ctggactggt atctgttagg tgagaagctg aatcagggcg
    11941 tacataccta tgtaaaggaa ctgttggaac tgtaccgcaa gtatcctgca atgtatgaga
    12001 tcgacaatac ctgggacggt ttcgagtgga tgaatgcgga tgatgcagaa cacagcacct
    12061 attgctttgt gagaaaatgt tccagcggca agaacaatct gctgtttgta ttgaatatga
    12121 ctccgatgaa atgggagaat tataccgtac ccgtgccgaa aaagaagaaa tataagctgc
    12181 tgctcaacag tgatgaggaa cgttttggcg gctggggcaa tgagattccc gcagagatca
    12241 tggcagagaa aaagccttac cattacaagg attattctat cagctttgat ctgccccctt
    12301 atggagctgc tgtatttctg ttctaagtcc ggttaagaaa ttataagaaa cgaccgaaat
    12361 aaaagatgag tgtgaccaaa aggtgcactc atcttttttc ttaacgattc agagacatgt
    12421 aaatatcact aaggcctctt tgaatgcttg cattcgtaag aggatttagt gatatttata
    12481 gggcgatata gcctcaatgg gcaaaaggaa agctctgaaa gagcgatttt gcgaatgagt
    12541 ctctgaatag ttatttcaag agttattacc aggaaggacg acaagcgtga agataacatt
    12601 tcagaatcag acggtaactg atacagaaaa tagaacagaa agtatccaga cggagaaaaa
    12661 tacctggggc agagataaaa tacagcgcaa taaaaaaagt gtgaatgcaa atacttttgg
    12721 agccgtatac gagagtgggc agatcccaat gcctgtggga aatgaagaga ataataaggg
    12781 aaaatccctg attgaactgc agcaggatgc cgggaacacc aatgtggcat tacagcagga
    12841 ttacatgaca ctactgtcac atactatgtc ccaggaggat tatgcgaagg cttgtgagga
    12901 cggatttgac cccagagaac tggatcagga taccgcagtt accatcgtgg acaggatcaa
    12961 agcagaactg gtgcgttccg gacagaacat tgccggatac acggacgaca tagatctgga
    13021 caccctggcg gcagctgtgg gcagcgatac actggcacaa agtattgaag aacagttccg
    13081 ggcaacggat atacctctga ctcctgaaaa tgtagatgaa ttaaaaaccg cctgggagct
    13141 ggcatcttct ctgcagcagc cccaggaagg cgaggtcggg tatcttgtag ataatggatt
    13201 ggagccggag atctggaact tgtatgtggc agagaacagc ggtgcgaaag tacagcagaa
    13261 taccgtaccg caggaattgc aggaccagat ggataaggtg atcacggatg ccggcctgcc
    13321 ggtcaatgat gagaatcgcc agaaagcgca gtggctggtg ggagcaggtt tgccactgac
    13381 aacggatacc ctgcagcagc tggcagaact ggatacgatt gactatcccg tcagtgagga
    13441 tgcttttgca caggcagcgg catcggcact cgcggagggg aaatcccctg ttcatgcgaa
    13501 tctggcacgg caggaaaata tatacgagaa agccacagaa atggtacagg actggttctc
    13561 ggatgcaaaa tgggatgtca cggcggagaa tctggcggcc agaaaacaac tggaagagat
    13621 ccggttgcgc atgacagcgg aagtgaatgt gaaactctta cagagtgatt tttccattga
    13681 tacggcccct atggagcagc tgatcgaagc actgcgtaga gcggaggcag aagttgcgga
    13741 taagtatttc cccaacgatg cggatgccgt ggcaaaatat gagacctata cacagaccgt
    13801 acaggtgaca aacgaactgc cgggactgcc ggtcagcgtg ttgggaccct atagcctgga
    13861 acagaaagtt tctcaggaga ccgtggcaga atttcacagc gaaggtaagg cgttgcagac
    13921 ggcctataca gaagcaaatg aacgttatga gacactgatg acagccccca gaagagatct
    13981 gggggacagc atccgcaagg cattttccaa tgtagatgac attctgacag atatgtcatt
    14041 ggataaaaca ctggagaacc agcgggcggt ccgcatcctc gcgtataacc ggatggagat
    14101 tactgcagaa aatatcgaga gagtcaaaga agcagacaaa caggtcactg cagtgattga
    14161 gaaactgaca ccgaagaatg tattacagat gatccgggac ggcgtaaatc cgttggagaa
    14221 gacttttggg gaactggagt cctattttgc cgaaaatcct cagagttatg aggaagaggc
    14281 ggaggactac agccgtttcc tgtatcagct ggaacaaaag aaagatatca cggagaatga
    14341 gcggaaagct tatatcggta tctatcgtat ggtacaccag attgagagag aagatggtgc
    14401 cgcagtaggc gcggtggtaa atatcggcgc agaattgcaa ttttccacac tcctggcagc
    14461 ttccaggagc agaagaacct cccatatgga ctggaaagtg tcggatgata ccggactgac
    14521 acaggaagta cgtttatcgg agaacaatat ttcagagcag atttctgtag ggatggcaaa
    14581 agaaatattg acagatgtgt cagaagacgc tgagagcaga gacgcatatt atcaggaatg
    14641 tctgcagcag atgcgggaag cggtagagac cgaaccggga gcagcacaaa tgttggaaag
    14701 gggagaggta aataccagta cttctaacct gctggcagct caggctctgc tggaggatcc
    14761 ggcggaactg ttcagcgacc tgcaacgcta tcgtaagaaa tacaacaaag agaaagaaat
    14821 acctgtggcg gcaaccggag gggagcctgc cggaacagaa gcctccggat tgtgggaaca
    14881 gctggatcag ccgaattttg cagatgatta ccggaccatg cttcaggatg ctttacagga
    14941 gacagagacg atcagtctgg aacaggcaga gagccatctg gatgtgaaac agttacaact
    15001 ggtacacaaa cagctgcgcc ttgcaggaaa tcttcagaac aggcaggagt atttcctgcc
    15061 gatgtatctg ggagaccagc tggcaggagt gcatcttacc ctacagcagg gagccggaga
    15121 gacctcggct gtggatatcc gggtagatgc gggagacgaa cagttggaag cgcatttaca
    15181 ggtcaacgga gaacacatcg aaggttatct cgtaggaaat accccggaag aggttacgaa
    15241 actggaaaag acctccgata tattccttga atggattcag acagatacct ctgcagactg
    15301 gaaagcggaa aagctttctg ttgtcagctc ccgggatatg acaaggatgg ctgccggtga
    15361 gacgcaaaat gcagaaacaa tagagagccg ggcagatgcg gcgcaactgt accgcctggc
    15421 aaaaggattc ctgcaggctg tcgcagacag cagcggaaag taaggagtaa
//
LOCUS       DAWDHD010000052        14872 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_272_length_14872_cov_9.466491, whole genome shotgun sequence.
ACCESSION   DAWDHD010000052 DAWDHD010000000
VERSION     DAWDHD010000052.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 14872)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 14872)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14872
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_272_length_14872_cov_9.466491"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         4381 a         2989 c         3936 g         3566 t
ORIGIN      
        1 ctgtaggcgt tattgaaaaa gtcctgagga ttcggatagt gatggaagct gttggaagag
       61 aagattgcat cgaaggtgtc cccagggaat ggaatgttct cgcagtctcc cacgacgaag
      121 ttggtatttg acaggttctt ggcctgggct acgcggatca tttccggggt cagatccagt
      181 ccggtgtaat gtttgtcggg atatttttca tgtaacagtt cgatcacggg accggtgccg
      241 caaccacagt ccagaacaga gtcaaagggc tctttttcca gttcttccaa aagataagga
      301 taatcatctt tacacatttc ataaatgccg gagtgaccgc tgtcgtagac tttggcggcc
      361 tcggtaaatt ctttgacgga gagtgcttta tattcttcgg atgtcattgg aaatcctcct
      421 gttaaaaata ttttgtaacg attcagaaac tctgaaagag cgattttgtg aatgcgctga
      481 atagttacaa ttttttaatt attaactgta cgaaaaaaaa caaatggtta gtcaaaacta
      541 acaatgaaaa tatagcacac attccataaa aaatccagta gaaggcataa gtttttcaaa
      601 tctgcacaga ctatccatga cgcgtggcgg gactgcatcg gctcttttgc cgcgtgtcct
      661 cttttaaaat tttaaaatca aaaaagaaga cgagaagccg cagaaagcgg cagaaggcga
      721 gggaaacgtg gcagatttaa aatgtgttgt ggaaaattgt acctataaca aggactgtct
      781 gtgcagcaag ggagacatca tggtgggagg taagcatgcc tgtgattgtg acggcacctg
      841 ctgcgagagc tttgcgcaga agagagaggg aagagagtct ttcagtaatt cgctgtctca
      901 tccaagccat accatcagta tcgactgtga ggcggtaaag tgcatttata atagtaatta
      961 taagtgtgtg gcggagcatg tggacatcaa gggctgcgga gccagcgact gcagagagac
     1021 cgcctgtgct actttttcgg aaaagtaaat cacggaaaag aaaatcagta ttccggcttg
     1081 acaaatgttc cgaaactgcg gataatagag caagtgatcg gattcggcgg gagaccgccg
     1141 gtacatagaa tacagtaatc tgaaaaaatg taatattatt gtaaaaacgg ggttgacatc
     1201 aggaaaactt cgtggtattc tatttgagcg tgtgaagaac acgatacaac aagcagagta
     1261 tccactctca ccttacggcg caggctggta agcgttcaga tttttcatag acagcgaact
     1321 cgtttggaat ttgtttgaat gatggcagtc cgtgaaggga acaggtggat agttatgcta
     1381 tctgcttttt ttattgttta atacattttc ttatggaggt acacaaccat tagcgattta
     1441 atgattaatg aacagatcag agacaaagaa gtacgactga ttggagaaga cggagaacag
     1501 ctcggaatcg tttcttccag agatgctctg aagatggcag aggaagccgg actcgacctg
     1561 gtgaagattg ctcctacggc aaagcctcct gtttgtaaga tcgtggatta cggtaagtac
     1621 aaatacgagc aggtgagaaa agagaaggaa gcaaagaaga agcagaaggt catcgatatc
     1681 aaggagatca gactttcccc caatatcgac accaacgatc tgaataccaa gattggtgca
     1741 gccagaaagt tcctgaccaa gggagacaga gtaaagatca ccctgagatt ccgcggcaga
     1801 gagatggcac acatgaacaa cagcaagcat atcttggatg atattgcaca gagcctgtcc
     1861 gatattgcgg tagtggaaaa agctcccaag gtagaaggca gaagcatgac tatgttttta
     1921 actgagaagc gttaattcgt acagttaaaa agtgccggga tccggcacga tagattaagt
     1981 aaagtttagg aggaactcgt tatgcccaaa atgaagacaa gcagagcagc agctaagcgc
     2041 tttaaagcaa caggaaccgg taagttaaag agatctaaag cttacaagag ccatatctta
     2101 accaagaaat ctaccaagag aaaacgtaat ctgagaaagc ctgcaattac ggatgcaact
     2161 aatgttaaga atatgaagaa gattttacct tatttataat tcaggcgagg aggaaataag
     2221 acatggcaag aattaaaggt ggcttaaatg ccaagaagaa acacaataga gtattaaagc
     2281 ttgcaaaggg ctacagaggc gcaagaagca agcagtacag agtagcaaag cagtctgtta
     2341 tgagagctct gaccagctct tatgcaggta gaaaagagag aaagcgtcag ttcagacagc
     2401 tgtggatcgc tcgtatcaat gcagcagcca gaatgaacgg tttatcttac agcaagttca
     2461 tgtacggtct gaagcttgca ggcgttgata tgaacagaaa gatgctggca gacctggcag
     2521 ttaacgatgc agaaggtttc aagacccttg cagagctggc taagtccaag atcgcataat
     2581 taaagcaact gttaaggctg gacacactgt tttcggatgg tgcggacagc ctttcttttt
     2641 ttgtaactat tcagaaccat ggaactacca cgaaatattc atcgaatgct tgcattcgta
     2701 aagatattat gtgatagtta tagggtgaca gaatagttac tgattagaag aggagactac
     2761 tatgataaat cccggaatga tgatgaaact gatgaatgcg aagaatacct tcgagagcaa
     2821 tcatcccaaa tttgcagcgt ttgtaagcag attttttatg ggcggagcga tcacggaagg
     2881 aactattatc gagattacga tcacaagacc cggcgaagag cctgtgtcca cgaatctgaa
     2941 ggtacagaaa tccgatctgg atctggtaga ggagcttaag aatctgagat gaagataata
     3001 ttttttgaca ttgacggaac cttaatatgc ggaggaagtg aattgatgtc ggagagcacg
     3061 aaggaagcta tccgcatcgc cagagccaat ggtcatatct gcatgatcaa caccggacgt
     3121 acgaaacggc tggtagggga aaatcttacc ggacaggtgg aattcgacgg attgttatta
     3181 ggctgcggaa cggagattga atatcatgga aaacagctga tgcacaagac gttttccatg
     3241 gaggagtcta aggcaattct tgcagccatg aaaaaatatc atgtggatgc cgtcctggaa
     3301 ggcagcaggg aagactatgc acccagagga gaagaagtat ttaccgagac cttccgtcat
     3361 atggcacatg agatcgagaa tcgaaaatac gatcgctatg ccaatgcacc gggtaactat
     3421 gataaattat atgcttatgc agagactccg gcgggaatgg acggaatgaa gcatgatctg
     3481 tcggaaatac tggattttat cgacagagaa caagggtttt atgagattgt ccccaaaggc
     3541 tattccaaag caactgcgat ccgttatatt acagaatatc tgaacattcc gatggaagat
     3601 acagtagcta tcggggacag caataacgac ctgccgatgt tacagtatgc ccacaccagc
     3661 attgctatgg gcaattccag taaacaggtg ttggaagtgg cagattatat taccacagat
     3721 gtagataaag acggcatctg gaacgctttg aagtggttgg gtgttttaga taagtaaaac
     3781 gattaagaat ctgacagctt gtactaaaat gtgcaaaatg tacaaaaaac agtggcaaaa
     3841 cttatatatt atgatgattt gcataaaacc tatttacaaa acacctatct agtgctatga
     3901 taagttcatc aaaaggtaaa acgattaact tacagaaaag aagaatacag cagtataaca
     3961 gccagatggc tgtttctttt ctcacaaaag attaaacgat taacctttgg aacgaacaag
     4021 gataggagaa aggtatggga catcacagga agcagctgga atacggaaat atcaggatca
     4081 cagacaagct gttcggtgaa tacgtgcaaa aggtcagtga gaagatcatt ccgcatcaat
     4141 ggaagatctt gaacgatcag ctggaggact ccgaacctac ctactgtatt cagaatttcc
     4201 gcgtggcagc gggggagctg ccgggagaga gaaagggggt agttttccag gacacggacc
     4261 tgtataaatg gctggaatcc gtagcattct gtattgccgg ggggcagggc agagaatacg
     4321 aagcactggc agataaagtg atcgaactgg taggaagggc gcaggagacc gatggatacc
     4381 tgaacaccta ctacacagtc aacgagccag acaaaaagtg gtcgaacctg gtggaaggac
     4441 acgagcttta cacggcagga cacatgatag aagcagcggt tgcttactat caggccaccg
     4501 ggaagacgaa gattcttaac atcgccagga aaaatgcgga tctgatctgc agagtattcg
     4561 gtaccggcga aggacagaaa aaaggttatc ccggacatca ggagattgaa cttgcgctgg
     4621 taaagcttta ccgggtgacc ggcgaaaagc agtatttgga caccgcatcg tatttccttc
     4681 atgagagagg gaagaagcca agctattttt tacaggagat ggaagacaga ggcggctggg
     4741 aatttttccc ggagttcaaa aattacgatc tggaatattc acaatcacat atagaaccgg
     4801 taaagcagaa gacagcggaa gggcatgcgg taagagccat gtatatgtgc tccgccatgg
     4861 cagacctggc tgtggaatgc gaggacaccg agatgctgga agcctgtaag cggctgtggg
     4921 acagcacggt gaatcacaga atgtatctga ccggcggcat cggatccagt ggttttcggg
     4981 agaggtttac cacggattac gatctgccga ataccacgaa ctattcggag acctgcgctt
     5041 ccatcgggct gatgatgttc ggacagagga tgagcgcagc caccggagag gcgggctact
     5101 acgataccgt ggaactggca ttgtacaata cagtgctggc gggcatcaac agagcgggag
     5161 acagatactt ttatgtgaat cctctggagg tcgtaccgga gttctgcacg gagcatactt
     5221 acatggatca tgtgaaggct gtcagacaga aatggttctc cgtggcatgt tgtccgccga
     5281 atgtggcgag gacgttagcg tctcttggac aatacatcta cgcacaggaa gaaaatgcgg
     5341 tatgtatcca ccagttcatt tcctccactg caaaggcagt cctggaaagt ggaaccgtgg
     5401 agatcggcat ggaatccaca ctgttgcagg atagtaaggt gaagatccgg acgagacagg
     5461 acggtgaagt aacctttaag atccgccgcc ccggatatgc ggggaaacag gagattacag
     5521 tggatggcca gacagcaaag gctgttttgg aaaaaggcta tctgctgata aatactccgg
     5581 cgggagagca tgaaatctgt attgatttcg gtgtaaagcc cagatggatc gccgccaatg
     5641 acgaagtgag agcggatgct ggaagatgtg cattgatgaa cggccccgtg gtctactgtc
     5701 tggaggagac cgacaacgga aaatatctgg cagacctgta tgtggaagag aacacagagg
     5761 tcacgaaggg gaatccgcag acggatctcc cgggagagat tccgacatta cagtatgaag
     5821 ctacaaggat ccataaccgg tccaccgaac agggaaacct gtacggagag ctgaaactgg
     5881 agaaagaaca gatgcacctt acagcagttc cctatgggct ctggtgtaac cgtacaccgg
     5941 gagaaatgct ggtgtgggtc aaggtaaagg tgtagaacgg tgaacgtgta agcgtttaca
     6001 atctggtgtt tacaacgaca acctattgtc gttataataa agaaaggtaa aggagagaaa
     6061 ggtatgaaaa acaagaaaat cgtaagtaca gtccttgcaa ccgcaatggc aacagcaatg
     6121 gtacttacag ggtgtggcaa cagcaatcag gcaccggcag catccgcaga tgcaggaagc
     6181 cagccggcac agacacagac gagttctgac agcggcagtg gtgagagagt acatatcacc
     6241 tatgcacagt ggggtaacga gacggagact gcagcaactc agaaggtggc tgacaagttc
     6301 aacagtgagc aggatcgtat cgaagtagaa gtggttaaga tcgaccatga tacttatgta
     6361 accaagctga atgccatggc aactgcagga gagcttcccg ataccggtat tatgagtgag
     6421 gccggtgtac tgaagttcgc agagaacgga ctgttggctg acatcagctc catgtacggc
     6481 gaaggcgatg caaagcctct ggatgcactg accttcaaat atcagggaac gcctgttgct
     6541 tattccgcag caaacgaggt actgaatctg tggtacaaca ttgatctatt aaacgaagta
     6601 tgtgagaaac agaatctgga tgtcaaggaa atgactcctc ccgcaagtgc agcagatgcc
     6661 tgggactggg atacttttgt agagacagca cagaagctta ccatcgatat caatggtaaa
     6721 aatgctctgg atcctgactt cgatcccaac aatattgata tttacggatg taccatcaat
     6781 accctgccct ggcagctgga agtatgggca ctgtccaacg gcggcggtta ctacagcaag
     6841 gatggttctg agtgcaccat caatgatccc gcaaccgtag acgcattaca gaagattgca
     6901 gatctgtctg aagtatatca ctgcgcacct cccgtaacca gtgcagcaaa cgctctggag
     6961 tcttctttgg gaagcaagaa ggtcgttatg gcaactgatg gtgcatggaa tgtcggcacc
     7021 ttccttgggc cgagcgctga cttcgaatat ggcgtaggtg ttcttcctta tatgaaggaa
     7081 aaggttacca tctgtaccgg tggccctaac gtagtatttg ctactaccaa gcatcctgag
     7141 gaagcaatgg agtggttaaa gtggtattat caggaagaga attcctggtc tctgatcgaa
     7201 gcaggtacct ggatgcctat tctggattcc tggtatactg atgctgagaa gacagataag
     7261 tggatcgata atcctaattt ccccgataag gatatgtata agagcgcagt tgttgactat
     7321 gcaaaggaca acatggtatc cacatcctgg tactatgtga acggcaccga tgaattcaac
     7381 gctactttgg atactgtatt ctccaatgta tgggccggac agcagaccat gcaggaagca
     7441 attgatgaga atctggaaga gttacaggga atctacgagg ctaacaacca gtaatactat
     7501 taccgcaaca atgtattggg acaacataac aggtggctta cagtgatgtg gccacctgtt
     7561 gttatcagaa gatttggaat tattcacgaa aataagttac tctttgcaaa aatgtgtgaa
     7621 gaaatgaggg ttacaatgaa aaaagggaaa cagaaaaata acgctacaac aagagcagga
     7681 agaaaggaag aactgaccgc atatctctgt ctgataccgg cattcctggg agtgaccttg
     7741 atcagctatc ttcccacact tgcagtattt gtattaagtc tgtttgactg gaacggcatt
     7801 acgaagccgg aatttatagg gcttgccaat ttccagcgta tttttacgaa agatatctat
     7861 ttctgggatt ctatcaaagc aactgtatgg tatgcttttc tggcggtgat cggtgccgtg
     7921 gtatactcac tgatcatagc gatcctctta aacatgaata taaaaggccg gacattgttc
     7981 cgctccctgt tcttcatccc gtatctgctg ccggccatcg gcgtatttaa aggatgggaa
     8041 tggttatatg aaggtaactt cggtctgttc aacttcgtgc tccggatgat gggacttccg
     8101 aagcagaggt tcctggacga cccgaatcag gttattccct gtctggtgct tatcgccata
     8161 tggaccagtg gcaacctgat cgttatcttc ctggcgggat tacagaatgt ccccggcgta
     8221 tacatagaag cagcgaagat cgatggggcc aatgcatggc agagattttg gaatattacg
     8281 atccccagta tcactcccat tatcttctac aatgtgttga cagcactgat cacacattta
     8341 caggtcatca acccggcact ggcgctgacc aacggcggac ccggtaaacg atccatgttc
     8401 atgtcctatg tgatctatat gtatggtttc cagaagaaca agatgggcta tgctgcggcg
     8461 tatgcggtga tcttcttcat tcttgtgggt atatttacca tagttctgtt caaaacacag
     8521 aaagaacaat tatttggaga ggaggaatag aagatggcag caaatttatc accgagtaaa
     8581 aaacggaaac aactggttct ggatctggtg gcaacgctga tcgtactttt gctggcattg
     8641 ttagtggtac tgccgatctg gtggatcttc cggtcctccc tgatgagtac ggcaaaattg
     8701 aacgagtatc cgccttcctt cattcctcat gagtggttgt gggcgaacta ttcaaaagcc
     8761 ttggagacct tcgaatactg gagatatttc cggaatactt tctccatcat cattccggcc
     8821 gttacttttg gtacggtgac cgcaattttc tgtggctatg cttttgcaag acttcgtttc
     8881 cgcggcaaga agctgatctt taatatctgc gtcggaacga tccttctgcc cagcatggtt
     8941 accctgcttc ctctgtacgt ggtatggacc aagggactgg gactggggga tacctactgg
     9001 ccgctgatcc tgccgtactt aaccggtggc ggtttcttca atattttcct gatccgtcag
     9061 ttcctcatga cgatcccgaa ggagctggac gaggcagcat ccattgacgg tgcgggcaga
     9121 atgcgtatcc tgtggacgat cctggtgcca tccatcaagc cggcagttgt ggtagtagcc
     9181 atgatgctgt ttatccagat ctggaacgat atgctgcagc agctgatcta cattaacagt
     9241 atgtcgaaat tcacttttgc cgtggcactg acgaacttta ccggatcctt cggcaccaac
     9301 tggccggtag cactggcagc gacgttcatg acaatcctgc ctggtatcgt gatctatatc
     9361 ttcggacaga agagtttcgt ggaaggtatc gttctgaccg gtatgaagaa ttaacacagc
     9421 ctgttgtgat atagggaaaa gttgtgttag aatggtggta gacaagtcgc ttacaaaagg
     9481 gagggacact atgcctacca taaaagagat cgccagagag tgcggagtat ccgtggcaac
     9541 ggtatccaat atcatcaacg acaagggaaa agtgggcgaa gagaccaaaa agagagtgct
     9601 gcaggtgatt caggagatga attacacgcc gaacatcgta gcgcagaatc tgaagacgaa
     9661 gaatacccgg agtatcgggg tcatcgcgga ggatatgaca gtctttgccc tgccggatat
     9721 catcgacggc atcacggagt attgcgaaaa agaagattat cagatcctgc tggtgaattt
     9781 acgtctgtat aaaaaatacg gagatgccta ttatcaggac aatatccacc ggaacatcgt
     9841 gcagaaggag atccagaagc tgctgtcgaa gcaggtggag gggatcatct atgtggcgtc
     9901 tcatgagaga ctgatcaatg tgatcccggc ggatctgccg attcctacgg tggtggccta
     9961 cggatttacc aacaagccgg agattccttc cgttgtggtg aaggacaaag gcggtgccga
    10021 gcagctggta cggtatctga tctcctgcgg acacaaacga atcggtgtca tcgccgggaa
    10081 aaaggaaagt atccacacac agtcccgact ggaagggtat cagagtgcgt tgttcgaagc
    10141 gaatctgctg tacgatcccg gaatgatcgt ttacggagaa tgggatcgtg aaagcggtta
    10201 tgagcatacg gaagaattgg tgaagcgggg agctacggcg attttctgta tgaatgattt
    10261 tatggcgggc ggagcctatg accgactgga tgaactggga tatacggtgg gaaagaacat
    10321 tgcggtggca ggctttgata acagggaaat ggcaagctat gagaagccgg agctgaccac
    10381 catgggactg ccgttacatg acatcggata ctgtgccagc gaggtgatct taaagcttct
    10441 gcggggcggg gagatacatc agaaaaacgg ggtgtattcc gtggagtgta agccgtttat
    10501 aaggaattcg gtgaatatga tataatgagc gcaagagagc cagagagagc ttgctcactc
    10561 tcggcgatca gaccggatta cgcatgtttg ttgtaatttc gggagttcga agaacgaaga
    10621 aatcatttat agaaaaactt gggagagatt ggggcatgaa acaggttaag attacattag
    10681 ataaggcata caaggtggcg aaggtggatg accgtctgtt cggttccttt attgagcatc
    10741 tgggccgggc ggtgtacggc ggtatctacc agccaggaca tcccacggcg gacaaatacg
    10801 gttacagaca ggacgtggta caactggtca gagaactgca ggttcccctg atccgttatc
    10861 ccggtggtaa cttcgtatcc aactttttct gggaggacag tgtaggccct gtggataagc
    10921 gtccggccag actggatctg gcgtggagat ctctggagcc gaataccttc ggactggagg
    10981 aattctacca ttggacgaag caggtgaatg cacagatcat gatggcagtg aatctgggta
    11041 ccagaggtgt ggcagatgcc tgcaatctgc tggaatattg caaccatccc ggcggctcca
    11101 aatatagcga ccttcgcaga tcccacggtg tggaaaatcc ctataatatc aaactgtggt
    11161 gcctgggtaa tgagatggat ggctcctggc agctgggcag caagactatg gacgaatacg
    11221 gacggatctc gcaggaagcg ggcaaggcca tgaaactgat cgatccttcc atcgagctgg
    11281 tatcctgtgg aagttcttat cgggagatgc ctactttccc tcagtgggaa gctaccacac
    11341 tggaacacaa ttatgattat gtagattatc tgtccctgca tacctattac ggcaatgcag
    11401 ctatggacag caatgattat ctggcaaaat ccgatgacat ggatgatttc atccgtacgg
    11461 tagtgtctgt ctgcgattat gtcaaagcga agaagcgcag caagaagcag atcaacttaa
    11521 gcttcgacga atggaatgta tggttccacg ccagcaagga ggatgccgac accacagaga
    11581 atcatccctg gcagaaagcc cctcatctgc tggaggatca ttataccttt gaagatgccc
    11641 tgatggtagg tctgatgatg atcacgctgt taaagcatgc ggaccgtgta aagatcgcct
    11701 gtctggcaca gctggtgaat gtcattgcac cgatcatgac ggaggagaac gggattgcct
    11761 ggagacagac cattttttat cctttctatc atgcatcctg ctatggcaga ggaactgtat
    11821 tgcagttggc tatcgacagc ccgaagcatg agaccagcgg ccacggaggc atcacagatg
    11881 tggaagcggt agcggtatgg aatgaggaag cggaggaagt gaccgtattt gccgtgaacc
    11941 gtaatctgga agaagacctg cccctgacca tggacctgcg cagtttcgaa ggctatgaac
    12001 tgatcggcaa gaccgagctg gtatccgatg acttacagac cgtgaactcc gcagcaggag
    12061 agaaagtaca gccccgtcag gccgacgatg ccaaagcaga aggcggccag ttggagaccg
    12121 tgctgaggaa agcgtcctgg aatgtgatcc ggctgggaag aaaataagat agtgacaaaa
    12181 gtggaaagct agttaaaaaa tgatagccct tggcagggta taagaaaggt ggccaccttt
    12241 tccttatacc ttgccggggc tattcgtttc ataaaaatgt tttctttgcc gaatttatga
    12301 aaaaacttct tttatacagt tcttttttca taaaaagtgc atttataacg gtttttatga
    12361 aatgcagaac tcctgaagcg gcagaaggtc ctccgggagt tctgtttgta tttaaaacga
    12421 caatttctgc gaaaaaaata caaaaacatg agattttgct atgtaaaaag tctctatttt
    12481 cgctacataa gcaaaataaa gaaattccgt acaaaaaatc ctggtttaga aagttttttg
    12541 tacggaattc attgttttga ggctttttac accaaactaa ctctccagtt acaacaagga
    12601 aaaagatctt tggctcggaa ataacgaaaa ttcaaataag cagataacgg gaatcgaacc
    12661 cgcctttcca gcttgggaag ctagcgttct accgatgaac catatctgca aatggaacaa
    12721 gaaatattat aactgatatt gcttcctttt tccagtattt tttgaaaaaa gggaacaaca
    12781 atgctaaaag agtttgcatc gaaagagcag acatgggatt cctctggcag aggttcttca
    12841 ttttccagac gggatactac agccggttca agggcatcat aagccatggt ataggcagct
    12901 tgaatgtctt ctccctgcgc catgcattct ggaaaatcag gaaaggtaat ccaaaaccca
    12961 ccttcttcag ctttgtgaat taatgcggaa taaaaaagta tattcaaagt atattcatag
    13021 atatcctcat ctaaacgtgg aacgggtgtg ataattaacg gtgtcatggg taatcacaaa
    13081 attattagta agaaaaaaca gaatatttca gaatagcatc agccgccgag tgttctacgg
    13141 gatattcaat aaggtgaacc tagacactgg cgatacgggc gaaccgcact cctcccggaa
    13201 ctgatgccaa ggttattata taacgatacg cagaaggaca caattggaag tgtcagtttt
    13261 gcgtattttt ttatgggttg acaataatcc gatttcggat tataataccg tttagtccga
    13321 tttcggataa aaacaaacag atgcaacgat gtagttacag gaggaaagcg atatggaaga
    13381 ggaaagcaga aaatcttttg cgcagatcac attgtacaat cagctgatca aagagatgga
    13441 tgacctgtac cgggtctatg caaaaaactg taatctgtcg gagacggctt tctggattct
    13501 gtattgtatc cgggaacggg aaaaagaggc gtttacccag agggagatct gcgactattg
    13561 gttctacacg ccccagacag tgaattccgc actgaaaaat atgacggagg aaggtctgct
    13621 tattctgcgg acggaggaag acaaccgcaa gaataagagg atatatctca cagaaaaggg
    13681 gaaagagact gtggagagga tcgtggttcc gctgatggat gcggagcgcc gggcattggc
    13741 ggcgatacga gagcaggagg cagaaatatt actgaaaact attaataaac taacggaatt
    13801 ttaccgggaa gaaatagaga aactgtcaat acaacgggat cccgcgtgat cgcaggaacc
    13861 tccgatgatg acacgaaaac gatgcctgca taattcgcag aagtatctgt tgagaaagtg
    13921 ctggaaggca tgcgggtaat agtaaggcat gaggttatta tgaaaataca gttatctgat
    13981 cattttagtt ataaacggct gctgcgtttc gtggcaccgt ccattttaat gttgttatgt
    14041 acttcggtgt acagtatcgt cgatggcttt tttgtatcca actatgtggg aaagacgcct
    14101 ttcgccgccc tgaatctggt gatgccggtg ctgatgggtg tgggaactct tggattcatg
    14161 gcaggtaccg gaggaagcgc cgtggtctcc atgacgctgg gagaagggaa aaaggaactg
    14221 gctaacgaat atttttcgct gatcgtatat gtgacactgg cggtgtcaat cgtggtggga
    14281 ttcatctgtt ttgcactggc accgcagatc gcactggcac tgggggctga cggagaactg
    14341 gaagcggact gcgtgcgtta ttccagaatc ttgtttttgt cctccccggc atttgttatg
    14401 cagtatttgt tccagagctt ttttatcgca gcggagaagc cggctttgag tttaaaggta
    14461 aatgtgatcg caggactgac caatgcggtg ttagattatg tgttgatcgt ggtatttcct
    14521 ttgggactgg tgggagcagc actggctacc gcagcgggac agctcgtcgg cggaatcatc
    14581 cctgtaatct attttttcag agagaacggc agcttactgc agctagggaa gacaaagtgg
    14641 catggaaaag tcatctggca gactgtgacc aacggatctt ctgagctggt gacaaatatc
    14701 tctacctcta tcgttagcat cctgtataac ttccagctga tggaagcagc aggcgagaat
    14761 ggtgtggcgg cttacggcat catcatgtac gtggacatta ttttcatgac cttgtatctg
    14821 ggatattccc tgggatctgc tcctatcgtc agcttccatt acggtgcggg ca
//
LOCUS       DAWDHD010000053        14839 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_274_length_14839_cov_10.946226, whole genome shotgun sequence.
ACCESSION   DAWDHD010000053 DAWDHD010000000
VERSION     DAWDHD010000053.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 14839)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 14839)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14839
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_274_length_14839_cov_10.946226"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         4043 a         3115 c         4140 g         3541 t
ORIGIN      
        1 acggaagtag tgtgcgaatg actgcgggac agagaaaaag gctccgtgag gttgtagaac
       61 tcatgaattc cctggcgctt cggacactgg cggcagcaca gagcggaaac actcccgggt
      121 gtggggaaga aggtatgaca ttcctgggaa tcgtggggat gcgtgatcct gccagaccgg
      181 aggcggagga agcggtggag attttccgcc gtgcaggtgt atctacagta atgatcaccg
      241 gggatcatgt ggataccgcc tatgccattg ccagacagct ggggattgca gggcatcgca
      301 gtcagtatat tacaggggaa gaactgaatc atctggatga tgcttctttt gccaggagga
      361 tcaggaatat cagggtttat gccagagtga ctccggccca gaaggtacgg atcgtgaagg
      421 ggctgcagca gaatggggag attgtggcca tgacagggga tggtgtgaac gatgctcctt
      481 ctctgaaagc agcagatatc ggtgtagcca tgggaaccgg cgtggatgtg gctaaacagg
      541 cggcggacat gatattgacg gatgataatt ttgcaacgat tgaaaaggca atcgaggaag
      601 gcagaggcgt ctatgaaaac atccgcaagt cggtgatatt tttactgtcc tccaatctcg
      661 gggaactgat gaccatgttc gtagcagtgg ctctggggct ggcttcccct ctaaaatcca
      721 gtcatatact gtggatcaat ctgatcacgg attcccttcc ggcgttggca ctgggagtgg
      781 ataaaaatga cggaaaaagc ctgatgcgct gtcctccaag aaaagcagcg gagagtctgt
      841 tcgcccgggg cggaatggcc tgtaccttac tctatggagc gctgatcacc ggtatcggtc
      901 tggcggcatt tctggtattg ccctgtggga tcatggcagg caggggagaa ctgtcctgga
      961 atctgtctga ctgggtggag ggacttcgtg aattactgtc ccgggaaaaa atacttgccc
     1021 gatcccagac ctatgctttt acagtactgg gaatgtgcca gctgtatcat gcagtaggaa
     1081 tgcgggatat gcagaagtcc gtatttgcca tgagaccctg ggataatccg ctgatggtgt
     1141 tggcctgtgt tattggattt gcgctgcagt ttgcggtaac ggaagtaccg tttctcatcg
     1201 gagcgttcgg aacctcccat ctgtccggac aggaatggct gctattgaat gtattggcag
     1261 catttccact gctggctcat gagctggtag tgatgttacg gaagtgagta cgcttatccg
     1321 ggcttaccga cgattcagaa cgataattcg ccctataact tcaggcagat attcttgcgt
     1381 atgcaagcat ctgccgaata ccctgcctga agctacatgg ctctggatag tcgtaatatt
     1441 cagaacccca ttcgcaaaat cgcatctccg atgctttcct tttgctcatg tggttacttc
     1501 gccacataaa tatagccaga ggatcttacg aatgcaagca ttcgacgttc ctccggttat
     1561 atttacgtgg ctctgaatat ttacaaaaag ttaacatttt ccttgtatgg agtttatagt
     1621 caatagttac aataaaattg acaaataact ttcatgggag ggcaatgtga tgaaagttat
     1681 cagaaaaaca gcgattgtta tgcttctctg cctctatttt ctgacatatg gtgtattgcc
     1741 gcaggtacag gccgcaggta aagacgctcc tgtgatcgtg gtagcgcatc gggcgggggc
     1801 aaaagtagct ccggagaaca cggtggcggc cctggaacag gccatccggg atggagtgcc
     1861 tattgcagag attgatgtgc agcagttgtc ggacggaacc ctgatcgtta tgcatgacag
     1921 taattttaaa cggaccacag gagaggatat ctgcgtatgg gatgtggagg cagatgctct
     1981 caaaaccctg gaagtgggca gcggtttttc agcagcatac cggggagaac agatcccgac
     2041 actggaagaa atgcttacct gtgccagagg caggatcact ctgatgatcg aactgaaata
     2101 taccggacag gaggatgcac tggaggaaag cgtgctgact ctgctgcagg attatgatat
     2161 ggtggatgag tgtattatag gctccatgaa caagggaatc ctgcagaaaa tgaaggagct
     2221 ggaaccgggg atttcaacag tttttattgc ccatgatctg gaagaagagg attatgagct
     2281 ggactatgcc gacagctata gcatcgaggg gagaaatctt acagtggata tggtagatgc
     2341 cattcattac tgcggaaagt cggtatacgg gtggacagcc aatacttccg gtgccatgct
     2401 tcggatcgta aactgtgggg cggacggtgt gatcacggat gatgtgagat tattacagac
     2461 atttttgaga gaacaggctt gccgtaaagc gtttgcaagt gtatactgat tagttataat
     2521 gaggggaaga atacggaaac aggttggtgt atatgagtaa aatatgggaa tttttcgaga
     2581 atctgggtga atatgtatat gtggcagatg cagagactca tgaattgata tatatgaaca
     2641 agaagactct gaagagctat ggatttcaaa gtcttgaaga gatcatcggg ttaaagtgtt
     2701 atgagatatt gcagggaaat tctctgccct gtggcatgtg taataatgaa cagttaaagc
     2761 cgggtttttt taaggaatgg gaatattaca atccgctgct gcgaagagaa cttcatatca
     2821 aggacacact ggtagaagaa gacgggagaa aataccgtat ggaaatcgcc atagattgcg
     2881 gcaatctgaa tgagagaggg cacaaatccg aagattaccg taatatggag gctgcgttga
     2941 atgaagccat ccgtgtggca cagcggcagg agagcatgga tcagacattg caggtgctgc
     3001 tggaatttgt cggaaagaat ctggaagcgg gacgggccta tatttttgaa aaggatgacc
     3061 acgatcatga ccacaataca tacgaatggg tagccaaggg agtcgcaccg gaaaaggata
     3121 cccttcagga tctaccgccg gagatctgtg cggaatggta tcggaagttt gatgagaatc
     3181 agagtattgt cacagagaat ctggaagaaa tgaaagatgt ggatccgcag atgtatgaag
     3241 ttctggtaag acagaatatt cattccctgg tggtggttcc gctttatgat gatgggaaag
     3301 taatcggctt ctacggagta gacaatcctt cgaccagata ttttgaattt gtgtcggaga
     3361 tgctgcagat catgggacac tttatcgtat cgtccctgaa gcagagaaat ctggtacggg
     3421 agctggaagt gatgagctat tcggatccgc tgacgacatt ggggaaccgt tatgctatgg
     3481 agaggtacat tgagcaggtg gaccataccg agcccatcgg cgtggtatac tgtgatatta
     3541 caggattaaa acggatcaat gacagtgaag gacattccgc cggagaccgc ctgatccgca
     3601 ggtgctgtga ctgcctgaaa cgtgtatttg aaggaaatgg aatgttccgt atcggcggag
     3661 atgaactggt ggtcctgtgc accggaattt ctgaaaagga attctatcaa aaggtggagg
     3721 aattaaaaac agagctggca gctcatgatg tctccatggc agtaggagca gcggtgaaca
     3781 ctctggaagc cgtgggaaag gatcatctac tgacggagtc cgagagacgg atgtatcagg
     3841 acaaggcaga atactatcgc agtacagggc tggaccgcag aaagatctga gaatggaaat
     3901 ctccaaagct attttgaaag tttatatgtt gagaatttct atcaggcagt tcccttgacg
     3961 ggaactgctt ttttctatat tatttatata ggttagctac gactaactca atgacttttg
     4021 gatggcagga ggtgaagcgc tgattgagtc aggaaacgtg ggagatcctg tgtgaaatgg
     4081 aagcagacga tgtggaacta cagttggctt tgcagtgtgc accgttgatc acgggactta
     4141 agatttccaa tctgctgacg atttccccgg aaggctttac acaggtggga aggatcgttg
     4201 aaagcagtgt gctttccctg tatcccttgt tggagacaga ggagaaaatg gtaatactgt
     4261 tgtatcggga agaccggctg gaaaagtatc tgaaaatgcc gcaggtacag aaactgctgc
     4321 gggaggcagg gtacgaatcc gatcgactgg aggatatttt gccggtattt tgcgatcgtt
     4381 atgaaaccta tgcagagaga gggaagccgt tcccccacga actgggattg ctgttaggat
     4441 atcccccgga ggatgtggaa ggatttatca gacatcaggg gaagaacagc ctctgcaccg
     4501 gttattggaa agtatatgcg gataaagaag gaaaacagag actttttgaa aaatatgaat
     4561 atgccaggga aaatttgatc cagctgttgc attatggatt aaagatgaca gaaatcatag
     4621 atatctgcgg atgtaccgca gcatagaaac aggcacggaa aggtgcggaa gggaaggaaa
     4681 aataaatgga taagatttat gtagtattct ggacacaggg tggtaacacc caggctatgg
     4741 cagaggccat cggagagggt atccgggaag ccgggaagga agcggaggta gtatttgtga
     4801 ccggcgccaa ccttgacgaa ctaaaggctg ctcccaagtt tgcactgggc tgtcccgcga
     4861 tgggagcgga agtcctggag gaatccgaga tggagccttt tgtatgtgat gtggaaggct
     4921 ttgcttccgg caaaaagatc gcactgttcg gttcctatgg ctggggagac ggtgagtgga
     4981 tgagagactg gaccacccgt atggaagcag caggagctac tgtgttgaac gatgagggcc
     5041 tgatctgcca tgagacaccg gacggggatg gcctgtcaga gtgtagagaa cttggcaaac
     5101 agctggcaaa cgcataaggt atctgaaagc aaataagaca acataacaaa aagattcatt
     5161 ccccgggtgg agagtgaatc tttttgctat atgtatgtgc tatgcttttt tgctgccaca
     5221 taaataatag cgttcctgtg ccacggtctc gggaaccgga atattaccgt tttgcagctt
     5281 tcgggtcaga acatccagat gatgtaagcc ttgggctacc ttgatcttac ggcgatccag
     5341 aagatcggca tacaacgggt tctgagccag tgtattgcaa atatcctcag ggaacgggca
     5401 gtaggtgaac atgatctgac gggctacttt gttcagtctc ggggaaccgg caccttcgaa
     5461 gagcacccag atcatataat cccttacgaa catttccttg aaactgttct tggcacgctg
     5521 caggctggat tttacctttt ccttggcctc tgtactcaga tcatggttct tgcggtagaa
     5581 ctggatataa tcgaaatatt cactggtcag ggaactgatg gagacatcgt tccagcggtt
     5641 accctggact ctcttgcaca gctcccagcg gaattcaccg gtcaggtggg tgaaagtcgt
     5701 cttcagatcc tccagatgga aaatggagaa gaccatacga ccggggctgt tacggcgctt
     5761 gccctcaatt tcctgccaca tgacaccgcg gataccgacg ttgggcatca ggatgatgtc
     5821 cggcagatat tccagatgaa ttgtctcctt acccatgaca tctatgtgct ccatatccag
     5881 actttctcta tagaatgccg tgtaatctac ccggcggatc tcctcgagaa tctgggagac
     5941 acgggaaacg gtgacatagg agctttccag atccttcagc acatcatctg cgcagaacag
     6001 cggacagaaa gtagtgatcc gaccgaaggt gatcttgttg acctgaggga acatattccg
     6061 gagctcgtaa ttgaccttgg ccatgggatt gttctccaat gccttgagct ctgcatccgt
     6121 aatattgcca ccgacctttt gtttgtgaat ataatcggaa taatcctggt caaattcgtt
     6181 ccggctggga gatttcttcc cgcggaagat ggccatcagc cagtcataaa aggtatagac
     6241 accgatatcg ctgtgatccg acatggcttc tgccatgcgg tacagggtga ctgcattttt
     6301 cagaccggcc agctcctcat ccacataacc gaaatacaga aacattttga ccggcatggg
     6361 aatgttcggc tgttccagcg tctgggtgaa caggagggag tagagcagat aaaattcttc
     6421 ggtcagctgt ctgcgtaaaa gagcgacatc ctgatcctgg gaagcccggt cggacagcag
     6481 cttgtaagcg tggacatgct gacggaagct ggtcacggta tccagatccg aaccggcgta
     6541 atccagaata gtgttcagcg atccggcaag cttgccaagg atctcggaat ctccggaaga
     6601 acctgcctca gcggaatcat tggattccat gagattcagg ctgctctgga aggtctggga
     6661 tctggaggca aacagttcct gatcgatgaa ggacagatcg ccggccttag aaagcatacg
     6721 gtgaatgcgg tcgtagaccg ccttgctgtc ttcattatcc tgtcccagtt tatagaacaa
     6781 agaagtataa aaatcaaaaa gatcgttgcc ggattcccgg aaatagaagg aaccgacctg
     6841 ctgcagatag tggaactgtt cctccaggct ctggtaagta cggcggaagt ccagacttcc
     6901 cttacgcagc ataccaatgg acagatccga ctcctgtacc aggatccggt aattgtccga
     6961 agccaggatc ctgcttaagc ccatgtaata gccgttcagc cagatgtccg gtgaatcatc
     7021 cccaagaaaa gcattcagct catcgaaatg ttccaaggat ctcgcctgca gacggtaacg
     7081 gctacacagt gtattgtagg tggcaatatc atctgtcaga gtacgataca gctcactgca
     7141 gttcaattcc gaaatggaac tgcggttcag cagaatattg atctgacgga aacaggacag
     7201 acagaacaga cgtgctacgt ccgggtgctt ctgtagaata tcattcaggg aatccagact
     7261 ggtcagggga tacgtcagga tcgttgtatc ctccaacgtg gtatagccca gcagatggat
     7321 ctcggagcag atctcacaga tactgatcac gtctcccttt cccagagtat aagagccgcc
     7381 gggatatgat acatctactt taccgttggt aatcagatgc aatgcagtca tgggctgccc
     7441 atagctatat atagttttcc ccttctccaa tacgattcca gccattccaa tacctcgtct
     7501 tatcatacaa atatttaata cacattatat accataatac caacaatttt ctactgtact
     7561 tttgatagaa atgtgataaa ataaaactac atatgtgtca cttttacatt gacagacgaa
     7621 aaaagcgatg ttttcggtag aaaacggtac atggaaaggt atattggggt tatggaacag
     7681 agcagtgaat acagagagca gttgctggaa tcatataagc aggcagtgag gccgttactt
     7741 ccctacctgc cctggctgga gcagagcgcc ggcaagagag ccagctctat ctacagcgga
     7801 caggatatcg gagtgaattc cgtgtctttt ccggtatatg acgggactct cctgaatttc
     7861 gtcagggaag cgacgaaaag tcccctgatg gatcgcaact atgcctatgt atacacaaga
     7921 caccggatcc gcactcatca ggaggagcgg gatattattc agaaagcagg atggaaggaa
     7981 tgggatatcc tcagaggaat cctgtcaaaa tatgtcttag gcggcagaac aaagggaaat
     8041 ctgtggagcg aggcggtatc ggagcagatc ttttatctgg tactgaaaca gatgcaggag
     8101 atcattgaat tctgggataa gacaccggcg aacggaaagc tttgagtggg gaaaggcagg
     8161 atagatccat gggagaaaaa tcagtacaga aaaagcgata tatcgtagag aaagcaagag
     8221 aagtatttgt ggagaagggc ttcaaaaaag taacgatgaa ggatattgta gaggcctgcg
     8281 acatcagccg tggcggactc tatttatatt ttgagaatac cagtcagatc ttcatggaag
     8341 tgctgcgcat ggagagtgag gaggcggatg acgtattttc cgacagtatt acggaggatg
     8401 ctaccgcagc ggatatcctg cttctgtttt taaaggagca gaagaaagag cttctgcgca
     8461 agaagaatac actgacgcag gctacctatg aattctattt tgaaaacgaa ctgcccaaga
     8521 gagacaatat tttaaaaaag cagtttgatt ccgcagcgaa gatcattgaa aaactgatcg
     8581 aggcaggtgt ggataacgga gagtttatct gcgaggactg tgagggaact gcgagaaata
     8641 ttatgtttgt gctggaagga ctgaagattt cggcccagac catcggcgtg acggcggaag
     8701 cggtggatcg tgagatcctg tatctgttgc gcggattggg tgtagaagat taataagaca
     8761 acgggtgatg atgcttcagg gaaagtttcc tgaagcattt tctgtgtaga aaagctgcaa
     8821 agagataaag agagggaaaa ggtaagtatg acagaaacga tcatcagcat ggaacttccg
     8881 gcgggaggac atctggcaat ccgcaggaac cggatccgtc cggagggaga agaacggaat
     8941 ttatccagaa tcagtctggt gtccggcatc cacggggatg aactggaggg acagtacatc
     9001 tgttatgaga cggcgcgaag agtaaaagag catccggagt atttgaaagg aatcgtagat
     9061 atctatccgg cattgaatcc gctgggaatc gacatggcca gccggacggt gccgagactg
     9121 gatatggata tgaaccgtat gtttccggga gacgcatcgg gggatatgat ggagcggatc
     9181 acggcaactg tggtggattc cctgatcgga tccgatattt gtatcgatct gcacgccagt
     9241 gatatttttg tgcgtgagat cccacaggta cgactcagtg aagattttgc ggaggcactg
     9301 cttccctatg cgaaaatgac caatgccgac atgatctgga tgaatgccac tgccacggtg
     9361 catgaatcga cgctggccca ttccatgaat ctgctgggag ttcccacact ggtgctggag
     9421 atgggacagg ggcatgacat ccacagatcc ttcggtaatc agatcgtgga cggtattttc
     9481 cacatcatga gtgaaatggg gatctggaca ggccctgagc caaaggtgca ggagcctgcg
     9541 atatccagtg acggtgaggt ggagtttatc cgcagcaagt cagacggtgt gttcctgccg
     9601 ctgatcgagc acaaccacta tgtgaaaaaa ggagatctga tcggggaagt ggtggatcca
     9661 tttgaaggga cggtgaagca gcagatccgt accgcaaaag gagggctgtt gtttaccctg
     9721 agagcttatc cggtggtcta tgaaggagat ctgctggcgc ggatcctgac ggagtgaaaa
     9781 gattatgttt gagagagata tttataccat acattcgacc tacagagaag atatgcgcat
     9841 caagggcttt cagttcggca agggcgagaa gtctgcctgc atcgttggcg cagccagagg
     9901 caatgagatc cagcagatgt atatctgttc ccaactggtg aagacactgc gggaactgga
     9961 aaacaacggc tgtatcagtg ccggcaaaca gattctggtg attcctacca tcaatgccta
    10021 ttccgtgaat atcagccgcc gctttttcgg tgtcaaggaa gcggatatca atcggagttt
    10081 tccgggaaat gattacgggg agccggcgga ccgtctgacc aatgcgttgc ttacggagat
    10141 ccgggactac gtttatggga tccagtttac gtccttttac agaccgggag aattcgtgcc
    10201 ccatgtgcgt atgatggaga caggctatca gaatacgtcg ttggcgaatc tgttcggact
    10261 tccttatgtg gtggtgcgca agccggtacc cattgatacc aagacactga attataactg
    10321 gcaggatgag atgacggccg ccttttcggt atacacgaat cagaataccc agatcgatga
    10381 ggtgagtgca agacaggcgg tggcagcggt cttacgtttc ctgaaacgta tggggctgat
    10441 ccggtacgag agtcattccg gctatatcag tcatgttata tatgaaaagg atctgagtga
    10501 tgtacctgcg aaccgggcgg gaattttccg gggaaaagta catgcgggag acgatgtgcg
    10561 ctatggaaat gagatggcag aaattataga cccttacgat ggaacggtga gggagaagat
    10621 tctggccccc acggacggca tcgtattttt cgcccacacg gaggccctga tctgcgagaa
    10681 ggacatcgct taccggctga tccacagact gcatgaatga cgaaccgttc aaaaaggcag
    10741 gtaaaatcta tctaaatttg ttgttttctg ctattgtggg aaaatatagt atgttgtatg
    10801 atactattca gaaccatgta aatataatca tatgtacgtc aaaggctcgc attgtaaggg
    10861 catctgatga tatttatatg gtgagataac cacatgagca aaaagtaagc atcgcaagat
    10921 gtggttttgc gaatggggtt ctgaatagtg tcgcatagta taaaatttcg gaggagagac
    10981 atgagcaacg taagacgtat ctatgtagag aaaaaggaac cttatgccgt caaggcaaaa
    11041 gagctgcacg acgaactgaa aaattatctg ggtatcgatg tggcaaaggt tcgtgagttt
    11101 atccgctatg atgtggagaa tatttcggat gaggtgtttg caagagcctg caagactgta
    11161 ttttccgagc ctcccgtaga tgatctgtat gaagagacca tcgagattcc tgcggatgca
    11221 aaagtatttt ccgtagagtt ccttcccgga cagttcgatc agagagcgga ttccgcagtg
    11281 cagtgtgtga agttcttaaa agaggacgaa gaacctgtga tccgtaccgc tgtgacttat
    11341 atgatcgaag gacaggtgtc cgacgaagaa ttttccaaga tcaaatctta ctgtatcaac
    11401 cctgtagatt ccagagagac cggcatggag aagccggata ctctgatcac ggtgtatgat
    11461 gatcccgctg atgttgctgt ttttgacggc ttcagagata tggacgagag cagtttaaaa
    11521 gctctgtatg aatctctggg acttgccatg accttcaaag atttcctgca tatccagaaa
    11581 tatttccatg atgatgagaa gagagatcct tctatgacgg agatccgtgt actggatacc
    11641 tattggtccg atcactgccg tcacaccact ttttccaccg agctgaccga tgtggaattc
    11701 ggtgagggtt attatcgttc tcccttagag accacttacc agagctatct ggataccaga
    11761 gaagagatct tcaaaggcag agaggacaag ttcgtctgcc tgatggatct ggcactgatg
    11821 gccatgagaa agctgaaaaa agacggcaaa ctggatgaca tggaagaatc cgatgagatc
    11881 aatgcctgct ccgtagtggt tcctgtggag atggattacg gcgaccatag agagaccgag
    11941 gagtggctgg tatttttcaa aaacgagacc cacaaccatc ctacagagat cgagcctttc
    12001 ggtggtgctg ccacctgtct gggcggtgcc atccgtgatc ccctgtccgg ccgtggctat
    12061 gtatatcagg caatgcgtgt caccggtgct gcggatccta ccgttcctgt atccgagact
    12121 ttgcacggca agctctccca gaagaagctg gtaagaggcg cagccagtgg ctattcctct
    12181 tatggtaacc agattggtct ggctaccggc tttgtaaaag agatctatca tccttcttat
    12241 gtggcaaagc gtatggagat cggtgccgtt atgggcgcag ctccccgcag gaatgttatc
    12301 cgtgagacct ctgatcccgg cgatatcatc atcctgttag gcggacgtac tggacgtgac
    12361 ggctgcggcg gtgcgaccgg atcctccaag gtacataccg atacttccat cgagacctgt
    12421 ggtgctgagg tacagaaggg taatgcaccg accgagcgta agatccagag actgttccgt
    12481 cgtgaggaag tcagccgtct gatcaaaaaa tgtaacgact ttggtgccgg cggtgtctcc
    12541 gttgccatcg gtgagctggc agacggtctg accgtagatc tggacaaggt acccaagaaa
    12601 tatgccggac tggatggcac ggaactggca atctccgagt ctcaggagcg tatggcagtg
    12661 gtagtagatc ctaaggatgt agatactttt ctgggctatg caaaggaaga gaacctggag
    12721 gctgttcccg tagcagttgt tactgaggaa cccagacttg tactgaactg gagaggcaag
    12781 cccatcgtgg atctgaagag agctttcctg gataccaacg gtgcccatca ggagaccaaa
    12841 gtaaaagtag atattccttc cgaagaagag aattattttg ataaatgggc tgttcccgct
    12901 gtaggtgaga aactggaaga gggcgatgtg aagggtgcct gggtggcact gttaaatgac
    12961 ctcaatgtat gttcccagaa gggtctggta gagatgttcg actcctccat tggtgccggc
    13021 agtgtattga tgccctacgg tggaaaatat cagctgactg agacccagac catggtagcc
    13081 aaactgcctg tactggaagg taagaccgat accgtgacca tgatgagtta cggcttcgat
    13141 ccgtatctgt cctcctggag tccttaccac ggagctgtct acgcagtgac agagtccatg
    13201 gcgaaaatcg tggcttccgg cggtgatttc agcaagatcc gctttacctt ccaggagtac
    13261 ttccgcagaa tgaccagcga tcctgagcgt tggagccagc ctttcgcagc actgttaggc
    13321 gcctacgatg cacagatcgg gttcgggctg ccctccatcg gtggtaagga cagtatgtcc
    13381 ggtactttca atgatattga tgtacctccc acactggtat cttttgcagt ggacgtggca
    13441 aaatatggcg atattattac tccggaactg aagacccccg gcaataagct ggtacgtttt
    13501 tctattaata aagacgattt cgacattccg atgtatgaga atgtggcaga attatatggc
    13561 aagatccacg aactgaccga aaatggtacg atcgtatccg cttatgctct ggattccaag
    13621 ggtgtggcag cagctgtagc aaagatggcc ttcggtaaca agctgggtgt gaagatcgac
    13681 gatgaggtaa ccacagacga cctgtttgac aacggactgg gcgatattct ggcagagatc
    13741 cccgcagaca agatggctgc actggaagag aaggaactgg attacaccgt gatcggtatc
    13801 gtgaccgagg agcctgtatt cgtatatggt gatgtgaaga tcaccatgga agaggcactt
    13861 gtttcctgga cttccaagct ggagaaggta ttccccacca aggcagtgaa gggtaccgag
    13921 gcagtggcaa gcaatgttta caaggcagac agtatttatg tatgtaagca caagatcgca
    13981 aagcctaccg tattcattcc ggtattcccc ggaaccaact gtgaatacga cagcaccaag
    14041 gcatttgaac gtgccggagc ccatgtggag acaagagtgt tccgtaacat gaccgcagag
    14101 gatatccgcg aatccgtaga ggaattcgag aagtccattg cacaggcaca gatcatcatg
    14161 ttccccggcg gcttttccgc aggtgatgag cctgacggca gcgcaaagtt ttttgcaaca
    14221 gcattccaga atgagaagct gaaggaagcg gtcatgaagt tgttaaatga gagagacgga
    14281 ctggcactgg gtatctgtaa cggatttcag gctctgatca agctgggact ggttccttac
    14341 ggcgaaatca ccggtcagaa ggaggattca cctaccctga ccttcaatac catcaaccgt
    14401 catatctcca agatggtata caccaaagtc gtatccaaca agtctccctg gttacagggc
    14461 gctacactgg gtgctaccta ctgcaatccc gcatcccacg gagagggccg tttcgtagca
    14521 ccgaaagagt ggatcgacaa gctctttgct aacggacagg tcgctactca gtacgctgat
    14581 cagaacggta atgtatccat ggatgaggaa tacaatgtca acggttccta ctgtgcgatc
    14641 gagggtatca catcccccga tggcagatgc ctcggtaaga tggcacactc cgagagaaga
    14701 gataccgctg tggccatgaa catctatggc gagcaggata tgaagatctt tgaatccggt
    14761 gtacagtact ttaagtaaat tgagagttag ttaaatacat tggcaaaatc cacgaactcc
    14821 cggagcatgc gatacacat
//
LOCUS       DAWDHD010000054        14745 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_277_length_14745_cov_10.452212, whole genome shotgun sequence.
ACCESSION   DAWDHD010000054 DAWDHD010000000
VERSION     DAWDHD010000054.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 14745)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 14745)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14745
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_277_length_14745_cov_10.452212"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3425 a         4045 c         3082 g         4193 t
ORIGIN      
        1 cggcttccat gccgtatcgt aattgtactg cgtgtccaga atattgtaat aagcatcata
       61 acgctttctc tgggcatcgg ataaactgga attgttcctc tggtcatcat aatattcccg
      121 aagcgcaccg tatcggtcca gtcctaccgc cgcacgcatg gtcttgttga actcatagcc
      181 ccagtaccag cttttaccgc ttccggccgt actgttgata tacgaactgc tgtcatgggt
      241 gaacaggaca ctcttaccat cttcgatata atcacggatt gcttcggata ctgccatgtt
      301 cttcaggctc tgatccttgc ttccgaactg atagttgtct ccgaatccca ggatcagcat
      361 atcgtactgc tggaaagtgg tgtatgccgc attgtaataa gcctcagact tctgttcatc
      421 ggtcttaagc tttttcaact gatcgtctga aatcaatccg ttgataaaag ttgaagtctt
      481 cacggaatca atagatacat taaattcccg gataccgctt aacagtgact ttacatcact
      541 gtcactttcc agattccaca gtgtcttgtc attagatgct tttctgtcgg acatcagctg
      601 caatacctga atatccactc tggaacctct gtccacagcc gtataaccgg acaccgccgt
      661 tctcaccaga gcatcggaat tatcatagaa gatcatcttc cagggcaata ctccctgata
      721 atcatccggc agcactctga ctaccttata ggtatatccg gtgcgcagct cataatgacc
      781 actgtcgttt ttcgccacct ctttccactc ctgcccggat ctgacataca gattcagtcc
      841 ggaaagctcc tccgtggaag aaagtgtctg cccgatcaca tcggaaccgg aaaaacggcc
      901 atcggaattc ttatcaatat acagtttgca atcataagtc gtattcacct gggacgcagc
      961 cgcatcattc gacagagcaa aaatatactg cagataaaac ttaccgtccg tcatcttcag
     1021 gaatgcatca ttgataccgt catcgttgta atgcagctcg gtgggcatat agtcctcacc
     1081 cagattcttt acccattcga tctgcagctt ggacagattc agatggttgc caaagtttct
     1141 ctgtctctct tctcttgctt cctttgccgc ctttgcggca gccagcgcct ccttactgtc
     1201 atccgccgcc ttttctgctg ctccgggtac taactggctc tccgcaaaga cattcttctg
     1261 ccagtattta taattgccgc tttcatctgt agacagggca aactgtatca gctggtaaaa
     1321 gtaggagctt ctgtccacgg ttttcgtatt tgcgcccacc ttaccatcga ttcccgtcag
     1381 aagtgcatcc gcaaatacta ccggataccc ggcggaaata tactgctcga actccttttc
     1441 tctggtggag ttcatatcgt ttccgggtcc ccgatactga ttggaagtgt gactcaggga
     1501 atcatccctc agacgcttct tgttatcatc cgattcatcc ttataatcat acagatcccc
     1561 gatatgcgta tagatcagcc cattcatgga actgtcattg taaacggtct gattgttttt
     1621 cacattcatg gttccggtgc gggcacccac atagatcata tcatatcgat cgttgagatc
     1681 ctctatcttg ccaatgaact ctgcggaagc catctgggta atggaaatac ttcctccgta
     1741 actgctctgc cccagaaatc ctttcacgga atctgcagat aaagctccgg ttccgaaatc
     1801 ataacagggc tccagatcca ataccgcaag gctgtccttt acctggcttc tgtgcagata
     1861 ccagttcata atatatctgg tgatggaagc tttgctgatc tcttcattca aagtgaccgc
     1921 cgtggcattt tcctgtgtga tcagaaaatt ctcgtagcgg atctctttca ggacatccgt
     1981 gtagatccat gctcttgtcg tatctgactt cagatccgtc agataatcct ctgccgcata
     2041 agctttggaa ctgtcgttgt tcaggtatac attttcgttc aggtattcct tcaaggtatc
     2101 caacgtcagg gtatccgcat ttgccgcctg cattgcagac gcataggatc tgatatttcc
     2161 ctctgccgcg cttttcagcg tatcgacact tacattttcc caataggaat ctccctgacc
     2221 tgcgattgcg tttgccacat tgtcatccga cacctgcagc agagtcaatg ccagtttcgt
     2281 caacacttca ttgctctggg tgctgttctt ggcataaaaa ttataatcca tgatcaccgg
     2341 cactctggct gcagcggttc tgatcccgct gtcgtccacg ccaaatgcca gctttgcgat
     2401 cacggttgcc gcagagacca tattggacgg ctctgccccg taatttccct gaccggaaaa
     2461 atagaacaga tccacatctg ccagatcaat tccacaataa gaatcctccg ctgtaatgga
     2521 agcatctgcc agatcagcca gttcctgcag ggtaagtgtt gtcacttcga tcttcagtgt
     2581 atcgatatcg tctccggtgg ctgtaccact ctcataacga ctggtaccgg acagattcag
     2641 gacatttttc ttgaaccatt ctttgttgta gaaaccgccg ttataggcaa tagtctccgt
     2701 agcatcctct ctgtcatccc ctacaaaatc atagctgttg gcggtgtcaa agcccgcttc
     2761 ccccttgtaa gtaacctgaa gggaacccgg tgtcaccata gtgtagctgc cggcctcatc
     2821 cttacgatag gtatcgatca gacggtaatc accgtttttg tcacttactg tcattgcaga
     2881 tacataatag tcgccgttat catttgctgt gaaatccaca agataggcac tggctatggt
     2941 ctcaaatgta tatgccttcg aaacatcatc ccattttgcc aaaagctgtg cgctatccgg
     3001 ctgtgcatga tccaggatgt agagattctg ggtaatatct ccggcagaat aggctgctat
     3061 cgcatctcct ccggatgtcg tgcgtgcctg cacaacagta cctgcatcat ttccggatac
     3121 ccccctggac gtagtcttta atccatctgg cagtgccgca tcggtaccgt atacaatctg
     3181 tccgagatag gtataaacgg cagtctcacc cgtacctatc ttctgataga ttgtcctgtc
     3241 cacataatct tttgcctgaa aactcgtttt atcaatagcg gccagatccg tctccgaaat
     3301 tgcatataag ccctgcgcat atcccctggc gctgtctgaa ccattgataa tcgtagtctc
     3361 cagtccgttg cttcctccgg tagatctatg ggaaaatacc ggagtcacgc ttttggcatc
     3421 ccggttactg tctccggcgg cctccaatgc actgtatcgg ttataacgtt ctcctgctgc
     3481 taccgtctcc ccattggcaa tctcacgata gaccgcatcg atctgttggt aattgtatct
     3541 gccggatccg tcctgtcgga atacaaagcg tcctcttacg tcctcggttc tggaagctcc
     3601 ggtgctttcg ctgtaggcag aaaaagtaac cggacctgtc gtcggaaact ttgcagcatc
     3661 cagactgtcc atagctgtct ggcgctctgt ggctgacagc atctcggaaa gctttctgcc
     3721 ctcagatacc ggctcctcgc cgcctgccag atagccgatc gtggcatttt ccttcgccgg
     3781 aacgatctcc agaatatgga aagtaccgtc agaagaagct accgtatcct gtaccagttt
     3841 ttcaataccc ggaagggttc tttcctgcgc actgacctcc atagagccat agctctgacc
     3901 ggctccgacg gccaaagcta tggtgaggat tcctgctatg tacttttttg aattgcgttt
     3961 ccaattcatg gtcatttatt atccttccat tcccaccact ccggattctt ctctacttcc
     4021 agtaaaagat ttctcttatg atccggatat cgttcatcat aaatggtcat aattaaataa
     4081 tcattttctt catgtgcgcc aatatcaaac tgtacctgcg aaaaagggag atgcatatta
     4141 tcaatttctg caaatttcgt gttggaatcc aacggcagtt ctccctggaa atatcctgcc
     4201 agttttgctg ctctatgtcc tttctctctt ccagactcaa aaccatacgc atgcagatca
     4261 taacgattcg tccaggtagg tctcagcacc gtaatcacat gctgatcatt ggcaaataag
     4321 atatccacta ccggcaccag tattaccgtt tcggccacta atgtggaatc atccaggctc
     4381 catgcctgaa gcttcagacg atattcggta tcccaggaaa tatagctaag tgctcttacc
     4441 tctgtagatt ctcccgtatt ttctgttgtc agatagcctt cctgatgaac attatcatat
     4501 gctccgtcat agacttcaaa accgacctga tatgattttt gtattccgtt ccataccttg
     4561 aaatcattat tcagctcact gggaatatct gcaaatctcc aatttacttc ccctttataa
     4621 tttcgaatct cgcaattaat ttttccatta cggtttaata caaactgact ggagtaaata
     4681 tacggttcct tcgcgtttac aaccgtcttc aggggaggga ttgtaatatc gatttctctg
     4741 gtaatcttaa tatctactga taaagctatc accgcacgta cacgtactac cgtatccgtc
     4801 atatttccac attccagttc tgcctggaac tgtcgaccat cagaatctgt caaaccggtt
     4861 attttgtaat tattggcata atcatctacc acactccact caatctgtgg ataaccattt
     4921 ctgtaatccg cccaggacgg cagacattca atcaatgcct tataaccgat cttttccgta
     4981 cttccttcgg taaaatcata tgtggtcaga ttcctggcaa tcagtgttgc atcatatttt
     5041 ccgtcaatgt tggtataggg taataccgat acctttgtgg atgcagacat tccgggatcc
     5101 gccacggaag tacaggttac ctgtagcaca agtgttccgg tcggctcata tctgtcagcc
     5161 gtcaaaagac cgctcccatt tatcgtggtt cctgccttaa gtccagttgc gtccactctt
     5221 gccacgctcc aggtcacccc ggcattttta gcctcttctg ttccttccat agtataagag
     5281 tactgcaggc tgtccccctg aatcacagaa gcatctttcg gttctatatc cagagaatcc
     5341 accatatgct tttgtaccgt tacggtagcc gttgccgcta tggtaggatc cgctacggcg
     5401 gtacaggtta ctgtcagaat attctctgct atggtctcat ccgctgccac actcagttta
     5461 ccgttaccgc tgatggtact cttacatgcg ctgccatcct gataacttac ggcccaggtc
     5521 acgccctgag caatggcttc ctcgtctccg gtgatctcgt aatcgaagac gaaagcctgt
     5581 ccctgtttca gatctgcgtg ctcgggaatg atacggatgc cgctgatcca ggaatagatg
     5641 ctggacggtt cggagctcag atcatttctt aacttgatgg tctcggaaat atcataggat
     5701 ttgtcctgat aggtaaagat catattcaga acgactttcc gcttatccag ttcactgagg
     5761 tccacgctga aactgctgac atgctctgcc atcagtgcgg gcttagccgc gtcaaaatct
     5821 cctaccacaa ctttgccttc gccgtcctta gtatttgtta cctcataaag atacacctga
     5881 ttggcatcgg cataatccgc tccggcaccc ccctgccagc ggatcatata gctgttgtca
     5941 ttattgatca gacgcagctc gctgacactg gcatttgtta cttccatgcc gttaacgtct
     6001 tttgccacat tgccatcaac atcaaccgct tcctgatctg tcagattgga aaagctgatc
     6061 cctttctcca catcaatgat cagatcgatc atctggttgg cggtcagctg ggcttcttcc
     6121 tgcagggtca gttccttgtt cccccgcata taggaattgt tgccggcgac cacgaatgct
     6181 cccaccgcac cagctactat ggtcatgatg gcaagagata cgatgatctc taccagagta
     6241 aatcctttct ttccaatacc catggtcaac ctccttactt cgtcaggatc ttgccggtct
     6301 tgccgaccgt tgtgatttct atggttctcg ttccgttgct tacgacgatt ttctgacaat
     6361 attccttcaa cacattgttt gccgtacagg tatccttctc aaaggccccg gatgcacggt
     6421 taaacaccag atgcagtcca tttgaagatg aaacataagg taacaggctg tcggatcctt
     6481 tcagttccgt cagggaatat cccgtggaat ctccttcaaa atataccttt atatcaatag
     6541 gctcttttcc cagtaccctg ttggatacct ccacatctcc ctgccggatc tgtgccgtat
     6601 attccttggt cgtatcatta tagctgataa cgcattccac ccgattctgt tcttttccga
     6661 gggtcagcac tctggtccgc tccagtgttg tcacaatttc gtccgcacat ttccggactc
     6721 tcctgccggt caaggatgta atggatgtgg ccatcacacc caccagcacc gccatgatcg
     6781 caatgacaat gatcagctcc accagggaaa aaccactgtt gttattcttt ctgccagttt
     6841 tcataaacta tcaaatctcc cagatttata tcactgttct tataggactt accgttactg
     6901 ctcaggtaac cgtctccgcc atttaaaaaa tctaccacat gatattcctg tgtcttaccg
     6961 gcagcatcta cgatcgttct ggaataggtc agggcatgaa gcaccgcatc ccggtcaggt
     7021 tccacattga cattctgatc cagtgtgatc gttccgccgg acaggatcgt tccgtggaat
     7081 tcatttgtca ctttgacatc tccggtggca atgatcagtc cactgaactc gccgaatccg
     7141 gcttccggat aggtataatc accctttaca ataactgcct tatatccctg ctccgcaagg
     7201 cttcctgtgg tattgccgaa atattttgac tccaccttat ttccggtact gccgtttaat
     7261 acagcatgga aggtcgttat ctccccttca tttaccagat tggtataagc attggctgca
     7321 tccgtcttct ccgaaggaag cagctgctca tagacatcga tcatcttctt ggacaatgcc
     7381 tggaatttca actgatccga tgccaaaagt gcctgatatc ctgcattggc accatcactg
     7441 gtcagtgtgg catccaacag cttctctttg cttcccgcag ccgcactgct gtcatacatg
     7501 gccacattgc ctgccagatg catgatgtac gctccgttct gctgtccacg cacctgaatg
     7561 gctttggtat acagaccggt ataggcttcc atggtctgct ggttggcacc gtaataatcc
     7621 tgaaaatacc ggttggcatt ggctctcgcg gtatcagagg tgctgtcaaa gcttacataa
     7681 taatacacca gcgtgatctt gctgttgatc cgcttgaaaa tcttctggat attgttctgg
     7741 gtcagtccgt actcggccag cgtatgacca ttcagggtag cggacggaat attcaatgcc
     7801 acttccgtta catccgcctg gtccctgatc ttgtccttat actgctccaa cgtgatcggg
     7861 ttgctggcac cgtttaacac ggtcttgccg gaagcatcca cgccgataca ctgcgccgga
     7921 acgaggtaag ccagctggtc actcttcaca gcgatagact gtcccatcat cacatcctgt
     7981 actccggtgt tgctcacacc atcctcactg gtaattccat cattggcttt cgtcagagaa
     8041 atgtaagaat ttccggccag cgtaagattc ttcaattcat tcagatagat ctccgtatcc
     8101 gtgccgttga ccagaatggc gctgctgtcc acgccctttc cgttgccgaa gccgatatac
     8161 tgtccggcat attctccgtc gatctgtgct ccggcggtaa atacattccg ttccccggtc
     8221 atcctgaggt ctccggccac atatacgttg tttccctgaa agctcaacgt tccgttgctt
     8281 cccgtattgc ccggaatacc tccgttcatg gcaatggaac cggcccatac ttcaccgtac
     8341 tgatctgtgg taagtgtggc tccttctccc agatataagt cggaagccat caccatacgc
     8401 ttgtcttcgt tttcattgcc ggtggtctct ttccattcca ccgtcacacc gctacccttg
     8461 ccggactctt cgctgcccac gataaaacgt tccgcataaa agctgccgcc aatgacattt
     8521 ttactgatca gcgtggaatc agaaggaagt gcctgaatat tctcctgcgc caccatggag
     8581 attcccgtaa tggaaggcag tgtcactgcc tgtgcaaaat ccatatccgg catcttcacc
     8641 cggatatcgg tctggatcac ggacacatag ccatcggtat ccgtataagt cactttcagg
     8701 ccgcagaagg aaagtccgtc ggtgtacaac tgaaaacgtc cggtaggaat atccgtggtc
     8761 tccactctct ccttgttgcc cgtcaccgga tccgttgtca ttacatatac cttcagactg
     8821 ccgctgctgt ccagagccat ccggtacact acttccccgg agtcattcaa tgtctctagc
     8881 accgtccccc gctttatctc cgaagacagg ctgttgaaca gcttggtcag atcatagttg
     8941 ttgatattca tacttcctgt cactgcataa taatcctgca tatccttcag gaaataatat
     9001 ctcagtttgt tctgcttctc ctctgcggag gttgcctcat aatttttcag gaccagttca
     9061 taggcacttc cgacagaagc ggacacccgc tctcccatag ccgtccggat ctcatccagc
     9121 acagtctctg cggagtaaaa gttgtccttc gccttgagat tgttcacctt catctgatag
     9181 ttcagcagtg acatatacat caaaacactg gctaaaatgc caataaaggc catggcaatg
     9241 atcacaacga ccattgctga gcccttattg tccgttcgtc tgtggaattg tcttaccctt
     9301 gaaaatattt tgggatttct tccgcatatc ttcatgtttc tcgtcttcct tccggtatct
     9361 cctctatctt gcgtccgtca gatcattcga tcacggttcc cgtcatggta agtacggggg
     9421 actgcactcc gatcatatct tttacataca cctgtacccg catgtcatag atacggttgc
     9481 gtcgctccgt cggtgtcaga ccgccgtccg cacccataat atccttcgcc acatctccga
     9541 cagcatttcc tgccgcaccg gacacgctga gatcattgaa aatgtattcc gtattgctgc
     9601 ggtctatcag attcgtccgc agtctgcagg cggaatcaat gctggctccg gcagaggaca
     9661 tccagcccgg ccaggtctcc cgcagttcaa atgccgcctt gtaattgttg ttccaggagg
     9721 aatacgggtt aaaaccggtg ctttcgtccg tgaagctgtc tattctctcc gctgacggag
     9781 tctgctccac caggaacaga ttcgtctgta cattcttcgg gttatttacg atcactttgt
     9841 ctaccgcaga agtcaccgca ctctgggatg cctccactct gggctgcagg cacacaatga
     9901 taatggccag cagggaaatg atcacctctg catccaccgg attcccgctc tccttctcct
     9961 tcttaagctc cgctgctttg gctcttaccg cctcttcatt ggaaaagatc acataatccg
    10021 agccgttctg gggataatct ccgctgatgc ccgtaaggat agcggaatta caatgataga
    10081 ctgtggaggc cgtcaccaga tacacatctg tctctacact ctctccgtct cccagatcca
    10141 gtaaaatgcg gctgctgtct atggatattt cgatggtctt ggtagtattc ctggcaatct
    10201 ccataacgtc tctgccgccg gaatacgctt caaaataacc gtaagcctcc tgtgcggcat
    10261 cttccgattc ggaatatacc gcattcaaat tgcctgccag gttctggata tttacaatat
    10321 ccttctcatt cagtgccgtg taaccgttag gatccagtgt tactaccaca tcataggtgc
    10381 cggaagtcgt cttctcatcc cggaaggtat attgccagat accatcttct gctgtgccgt
    10441 ctccgtccac atccagagaa gcatccttct gcacgctgtt agtaaagtcg gtaggggttg
    10501 ccgcctcata ggtgtctgcc atctcctcca gtgtatgtgc ctcgaactgc tccatgacat
    10561 cctctgcgat ggccgtggca tgcatggtgt tgcgggactt ggcattggtt ctggcagaag
    10621 tcacaaagct gtgcaaaagc ggcacaacga tgatcgacag gatcacgatg gcaatcagca
    10681 gttcaatcag agaaaagcct tcgttattca gttgtcttct ctccgtgtgc ttctgcatgt
    10741 gaggcatcct ccctgctcat gactaatacc gttttctccg cactccgtgc tcccgaaacc
    10801 ggacaaccat tcgcactccg gactatcgtc ctccctgctc atggttgttt gatttgctaa
    10861 tcatagcagt atcctgcctg caagcagtcg tatactgcta tgattgcaaa tctattattc
    10921 cacgtttccg aaaatagtgt ctacgcccat ctgcatatcc gggatgtaag gtgaactgta
    10981 taccttatcg gttcccagca gataataggt gttcatggtc atgttgccgg taagcattcc
    11041 gtcggtctcg ttgtagctga tgctgacgcc ctggatgctc tcgcgttcct gctgattgta
    11101 aatgtatctt accagtccct tgaaatcgtc ataggtgcag gaaatgctga tgctcagggg
    11161 tacctcatac atatgggtat ctgcataagc agtaccgtcc gtagataccg gacttgttgc
    11221 cgtcgttgcg gcagctgctg tggtatctgc ggcagcatct gttgctgtcg tatccgtggc
    11281 tgctgttgca tccgcagtcg tcgttgccgc atccgctgca ggagtataca ccacctctgc
    11341 ttcggaaccg aatgcaatat ttgccacctc ggtacgagta taatcttcca atgtctttac
    11401 catcataatg ctgtcttccg gtaagatatc tgccgggaag cggttctcaa aattcgtgat
    11461 ataggactgc atcttctcgg ttgcttcaat gtagtccgcc ttacgctgat ctaatatctc
    11521 cagcgttgcc acctgggatg ataacgttac attctgtgcc tcaagggcat ctgatttttc
    11581 attaaaactg gaatatacca gaaaataagt acacacagca ataagaacac cgatcactgc
    11641 gatcaaaagc tgaatttcac tctttttaac cttcatcttc cgctgcctcc tactgtgctg
    11701 tctgggtgtc ggcctgtgcc tcaccgctct cttcggtctc atccgtcagc tccttatagc
    11761 ttccggtaat ggaaaaagtc actatggacg tgccggtctc atccttgctg tccgtaatat
    11821 tgctgatctc tacatcatca atgctctgca tgttgcggat attcagaatc gtacgggctg
    11881 ccgctgcttt cccttccacc gtcactgaca tgctgatgcc ggtctggtcg gagctgaagc
    11941 tgttggtata gaagctgtcc ggcaggatct gttccagttc attgatgaac tccaccagat
    12001 tctcattggg agtctgggta taagcatata aatatgtgta tttatcatac tgggcttctg
    12061 ccgccagata atcgttgtat actgcctgtg ccggctgcag ctcctgtacc cggttctgca
    12121 gatacacatt ctgcgccaca tttcccagat atctggtcac agaggtggct gccatggcga
    12181 tggacaggat cactcccaac accagtaccg ccacactgac gaaggtataa ttcgtgccgc
    12241 tgaccacggt cagtcccttg gacttctggg tgctcttgtc gatcagtccc acaggggcga
    12301 gcaccgcacc cagacaggaa atataggctg cgggtctggt ggaacggatg gccttgctca
    12361 tgccgatctt gtcatccatc tcggacaggg tatgtacctt gcgctccaga cagttggtga
    12421 acagcttgct catgccgctg aagctgccgc ccagaccggt aacataggac ttttcaatgg
    12481 gattttcact gttcctggaa tcatagaagt caatgacacg gctcacaccc acgatcagag
    12541 gctccaggga agcggtgatc ttctgtcttg ctgctgtgac ctccacattg ccggcatcct
    12601 catcttccca gcgtccggca ttttcttccc agactttgtc tccctgatgg agtactctgt
    12661 tcagacatgt tttcttctgg aaacgttcta ccgcactcat gggatcgttc acggcatagg
    12721 ctccggaagc gatcatagta tcaatagcat cctgcacacc gtaagccacc gttctctgta
    12781 ataacacctt accggcggaa agcacggtca caatggtgct gttctcatcg attttgatga
    12841 ccatagtaac tttttcggac ttctcatcgc ggagaatctg gtataagctg ttgctgctgt
    12901 agtcgaagca ctccacctcc cagccgcaca tctgcgccag ctgataatag ctgtttaaaa
    12961 gagccttggg tacggccaga gccatgacac gaagcttgcc attctctgcc agaccgcctg
    13021 ccagataatg tccgatctca tattgggtca gatccaccgg gaaatagtca ctggcattgg
    13081 tcttcaccag tgcctcgatc ttactcgcct tgacattggg aatctgtacc tcacggcttg
    13141 ccactctggt agaagaaatg gtaaacagca ctttcttcgt acggatgccg ttcgttccca
    13201 gtgcattcac cagatggtct gcgtactcct gtgtcggctg cagcatacca tccaccacga
    13261 tgccttccgg tgtctccacc gtcaatacct tgtagacctt cggcttcttc accttgtagt
    13321 ccatctcgca gatcttcgta aaagagttgc cgatttcaat actcaatacg cgattgttca
    13381 ttgccatatt tctacccttc ctcttcccgg tatttatcgt atttcaaaag ccttccgcca
    13441 tcgcagaatc tttgaagaac tttctgaaat catgttcgaa atgcaggtat cttcttataa
    13501 gtaaaaattc agataccact gtaagaattc attaccccac aggacagcga ttgccacccc
    13561 tgcggagaga tacggtccca tggcgaggac atgtccttcg ccgcttactt tcatacggat
    13621 cagatggatc acggaaccga tgatacatcc cagtaaaaac gcaaaaataa tgagtttcca
    13681 gccgagcaac agtccacaga ccgccatcag cttcacatca ccgccgccaa tggctctgcc
    13741 accggtagcg taatacagaa taagtaaaaa tacactgaca cacagaaagc cgatcaggta
    13801 atccagccac tgtgtatagt ggaagattcc atggatcagc cccagtgcca gtataaataa
    13861 gttaaatccc acagggatct catatgttct gaaatcaatc acacttaaag caagaagtgc
    13921 actaaataac aagcagtaga ctagggaatc cacgctcatc ccatatttcc agaatacgat
    13981 caggtacagc actccgttca gtgcctcgat gacggggtac tgaacggaaa tgtgtgcctt
    14041 acatttccga catctgcctc ccagtaccag atagctgaac aggggtacga ggtcgtacca
    14101 ccgcagctgg taaccgcaat tcatgcagtg agacctggtc gttacaatat tctctttttt
    14161 cgggatacgg tagatcaaca cattaaggaa gctaccgata acgatgccat agaggaaaac
    14221 cactatgtag agtaagattg ttgggagcat gtcaggtgcc tttctatttc agttacggag
    14281 tagcagtcag agtagatgca cctgctttat ctaccttaaa gctattcgct gcctttttgg
    14341 tatctgcata agtaacagta acagtaacat cgctatccgt tgatgataca ctatatgtaa
    14401 tagctgcacc cttttcatac tttaatgcat cttctaatgc gtcaccattt gtcacaccca
    14461 ttataccata aacttctttc cagaatgtat ctgctgtatc agggatatct ccaccagcca
    14521 gagatgaagt atgatcaata tccggatcca acatggtcgt agtaatagcc tgtctcaggt
    14581 tgtcagccaa ctgcttatca gcagaaatct tagacttatc cagatactta atgtactgag
    14641 gtgccagtac accaaccagt actgccatga tagcaataac aatgatcagt tctaccaggg
    14701 agaaaccttt gtttgttaag ctcttctgtt tcttgttcat aataa
//
LOCUS       DAWDHD010000055        14729 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_278_length_14729_cov_10.354505, whole genome shotgun sequence.
ACCESSION   DAWDHD010000055 DAWDHD010000000
VERSION     DAWDHD010000055.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 14729)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 14729)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14729
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_278_length_14729_cov_10.354505"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3289 a         4218 c         2864 g         4358 t
ORIGIN      
        1 ccacttccct cggtagtacg cgcagcatct ccccggataa atctctctga cagtacctca
       61 cccttgagat tcatgggccg gtcggaaata ttattcagag aagcgatgac tcttccgtcc
      121 tttacctgca tctccagata gactctggtt ccctccaggg catacttgca gatattctga
      181 aacagatttt caatgattct ccacatcctg cggctgtcgg catagatcat gccggccaca
      241 tcatttccgt caaagatcac ctgcaacttt ttctcttcca ttctgtcgga gaattccccc
      301 actgcctgat tcagcagttc cgtcaggttc aactgttcca gattcagcac aatgttaccg
      361 gaagagattt tcgaagcctc caccagatca tccgtcaact gtttcagtct ctgggatttg
      421 ctgtccagca cctcaatata gctcctggcc ggttcttccg tgatctttag gcgtttcagc
      481 agatccacat aattgataat ggaagtcagc ggtgtcttaa tatcatggga gacattggtg
      541 atcagatccg atttcatctg ctcatccttc atgctggtgc ttaccgcctt gcggataccc
      601 tctccgatgt tattgactgc atccgccatc tcacggttgt ccccatgaag agattccaca
      661 tccagcttat aatccacttc tccatcacgg atcctgcgga ttccttccac gatatcgatc
      721 tgctccgctt tttgcttgaa ccttagcacc cccacaatgc cgtccaacac aatagccccc
      781 agtgtcggaa gcacccacag agcgcttttt ccccgcagca gataagctcc gaaaattcct
      841 gccagattca ccagtagaaa cagattatac gggatcagtg agctgatggc ggaatttctg
      901 tgggaaacca caaaatgtac cgccttaccg aagcttgtga cgatccagtg caggaaactg
      961 tcactccaca tattgtggga tttgatcctg cggatcaggc tgtaccagaa aacattgatt
     1021 cccatgctta cgcagaagcc atacagcgca aagacgccgt aggctgtcag ccgtgtaatc
     1081 tcccgcccct ggatctcgga atggctgaga tataccttat tggccacatt catcagataa
     1141 tcgtatccga ttcttccgcc atagataaat cccaccagca aaagcgccat gaactcgatc
     1201 cagacatgat cgataccgtt caggtacaga accggctcat cctcctcatc gtaagccaca
     1261 ccggccgtta cggtaagata aatacccacc agcagccaca atactgccag caggatccct
     1321 ccgccgatca gataccagat attcggaacg attctctgga acaccacatt ggcattgtaa
     1381 aaggaatctc cctccaccgg ataattggta tccactgcaa tccagatatg agtcatatcc
     1441 ggatgcacat aaccatactc tttcagatac tgatagatct ggccctcagt cattcctgtg
     1501 ttaccggtaa attccagact gtccggataa tagatcagat accgtctgta ctccgaaaaa
     1561 taatccgtaa tatcattatc ggaactgttc tcaagctccg atacattcgt ataggttctg
     1621 ctaactccat ctgttgtcat catacggacc acatatttca gattgcccgc atcttcttta
     1681 tacagatcat tacagttctg atactgctca tagttggctg acaacgtctg gatcgtcatc
     1741 tccagattgt tctgcagcgc aatatattcg atccagttgt ccgcacaggc tgtcagctgc
     1801 ttctctccgt ctacggtggc ataacggttc accagcatcg ggaaatatac cgtcagcgta
     1861 ccatcgtctt ctctggatac ccggatatcc tgtacactct ctttcacaat gtatgtgaaa
     1921 gccaggtctt ccagccgatc ggttctctgg ctctccggaa ttgcctcgat tgcctgtgcg
     1981 actgcttcct tctgctcctc cgtctgctct ccgcctgcca cgaacatcag ctgtccgtcc
     2041 gcatccacgc ggaagttcga ctccaggtct atcggtccga aataattcac gaagtccgac
     2101 atactcataa tacgatctgt atattccgca ccgtattttc cccatttgat cagatcttcc
     2161 agctcataaa ctgctgttac agggcaatct gcaccgccgg ttttccctga agcaaattcc
     2221 gtgatatcaa catgcttatg cggatcaaag cttccattgg tctccatctg tcctttgatc
     2281 accataagct ggataatatc cgataccgag ctctggaaca gatcatggaa aatcacggaa
     2341 tcttcaaatt ccgtttccgc atccagaggg gataattcat atacttttgt gccatccgct
     2401 gtgtctaccg ccagatagga attgaacaaa accgccgcga ttgccaccag caaaagcaaa
     2461 gccgccgtct gctgcaccag tcccatcatc agccgtttca atgtaggtcg tctcatgcct
     2521 gctccttgtc gatcttatag cctactcccc ataccacctt cagataccgg ggttccttgg
     2581 gattgatctc gattttttca cggatatgcc ggatgtgtac cgccacggta ttgtccgctc
     2641 cgatggcatc ctcattccag atgctttcat agatctgatt gatggaaaat accctgccct
     2701 ggttcttcat caaaagtaat aaaatattat attcgatagg tgtcattttt accggttcat
     2761 cgtccactgt cacctgtttg gtatcatcat tgaggatcag tccgcccacc tggtagattg
     2821 ctttgttctc accggtcagg ctccccaggg aggtatatct gcgcaagttg gatttgactc
     2881 tcgccactag ttccagcgga ttaaaaggct tgcagatata atcatccgca ccgatattca
     2941 gccccaggat cttgtccgtg tcttccgatt tggccgacag gataataatg ggaatgctgc
     3001 tgtattcccg gatcttcaat gtagcccgta tgccatccag ccggggcatc atgatatcca
     3061 tgatcaacag atggatatcc tccttgtcca ggatctgaat tgcctgttcc ccatcatacg
     3121 ctttatatat tttatagcca tcctgactca gatagatctc gatggcatcc acgatctctc
     3181 tgtcatcatc acatacaaga atatttgcca tacttccctc atttctccgt tactgcccgg
     3241 ttccccatca ttttcgttat ttctatttaa acataatatt tttaaatatt ttcgtgtttt
     3301 ttcttaaatt ttcttaattc ccgtcaggca ctcaggaacc caagactctt tcagcattaa
     3361 aaatgctgcc agatttcccc tctgtccgtg acttgcccgg tgggaaaaca gatactccgg
     3421 attgtggcag gtacagacgt tcgttaccgc catatgttcc ggctggatcc ccgcccttaa
     3481 cagaatgctc tgattggctt tccacaggtc aagctgatat ttacccggtg ctttgcccgg
     3541 ctgcaacaca tcctccggaa agccttcccg gaactgttcc gccacttctt cactcacttc
     3601 ataacaatcc acacagatgg acggtccgat ggccgccacc agctcctccg ggcggctgtt
     3661 aaaatgttcc tgcatatatc gtaccataca agctcccatt cccgctaccg ttcctctcca
     3721 gccggaatgc gccagtccga tggccctgtg taccggatcc acgaaataca gaggaacaca
     3781 gtctgcataa aaagtcacca gtgcgattcc ggggatatcc gtagccagac cgtctatatc
     3841 gtcatagacc gatggtctgg tgatcccatt tccggcatct gctttcgtca ccagatgaat
     3901 attcgtggta tgggtctgtt tcgatgccac catatgttcc gggtctgttc ccagtacttc
     3961 accaatccgt ctgtaattct ctctgacaca ttccaggtca tctcctctgg aaaaactcag
     4021 gttcatagtg gaatagattc cgttgctgac accaccggtc cttgtggtaa acagatgttc
     4081 caccacaccg gtctcttcca gcttcgggaa taccagatac tccagttccc ctttttgtct
     4141 ctgctgtata ctgtctgtgt ctctgcagtc tgttctgatc aatggataca ttattctatc
     4201 tccttgcact ataatggtgt ggaaaagcat ttggaatcca gtgaagtgcc tccccctgga
     4261 ttgccaccac atcatagcgc acccaggtat cctccccata atgatgcaga taacggtaat
     4321 agtccgccac tctgcagatg gttctctgtt tcgccgttgt cacggcctcc gccgcatttc
     4381 ccttttcatc accggaacgg tatttcactt ccacaaacac aagatatttt ccgtctctgc
     4441 cgataatgtc gatctcaccg ttcctgcctc tgcggaaatt ccgttccagg atacgcattc
     4501 cctgtttctc cagatagtcc gcagccatct gctcatatac attccccgtc tgtctggtat
     4561 tcaaccctta tgttttcccc cgtttgccac actttccttt accatgcggt gatccacagc
     4621 atccacaaat accagaatct ccgccaccac ttcatacaac tccggcggga tcatctcgcc
     4681 gatgtccagc cgggacagtg tatccgccag cttgtcatcc cgatggatcg gcacactgct
     4741 ctcctgtgcc ttctctatga tcttctccgc cagaattccc ttgccgctgg ctatgactct
     4801 gggtgcctca tccgagggat cgtattccag tgctatggcc tgttttactt tctttttttc
     4861 gtcatccatt ctgttgtcct ctatgctctt ctaacatata cgttttctcc gtgctacgca
     4921 ctctcgaaat cgtaacaatc attcgtattc cggactatcg tcctgcatac tcatgtttgt
     4981 ccgctcctct cataggaaac atccgctgcc tgccagcagt cacgtctgtt cttcattgac
     5041 cttacaaaac atatacgttt tctccgtgct acgcactctc gaaatcgtaa caatcattcg
     5101 tattccggac tatcgtcctg catactcatg tttgtccgct cctctcatag gaaacatccg
     5161 ctgcctgcca gcagtcacgt ctgttcttca ttgaccttac aaaacatata cgttttctcc
     5221 gtgctacgca ctctcgaaat cgtaacaatc attcgtattc cggactatcg tcctgcatac
     5281 tcatgtttgt ccgctcctct cataggaaac atccgctgcc tgccagcagt cacgtctgtt
     5341 tcctatggat gagctatgtt ctcacatcaa aggcatattg tgctaccgga acacttcctt
     5401 ctgtcttaag aagcggtgtc atggcctgcg ccgtctgctg taattcagta tgcagtgttg
     5461 tctcacattt acaatcatag cctctttttt gcagccgctc tgtcagcaca tccatattag
     5521 cctccaggta atccaggatt gcatcattct gtacataaaa tttggtgctg actttggtat
     5581 ccttcagagc cacatataca tccagtggcc ccagatgttc catatccaga tgcagcagtg
     5641 cgctgacctg tccgtctttt ctggcaagat ttttcttgtt ggtatataca aacagttctc
     5701 ccgtcttatg ctctccctgc cgtagatgca gcggcagctg gatataggca taggtctggt
     5761 tgatctgttg caggaaatcc acgttctggc ttaaattcgt cacgttctga taagcacttg
     5821 acccacgctg tccgttttct tccaaaagat tactcaaccc cttcaactgt tttcccagct
     5881 tctggtaaag ttcctccacc ttctccggag attccacatc ctctggtgtt atgctccaac
     5941 gatccgccag aagttttgcc ggaagctctg ccactttgga attatggaga atactgcgca
     6001 gcaagggaat atctttcgtc tccagcccct gctttaacag tttctgcaga ttctgcagct
     6061 gtggtgcctc ctgtgttttt tctgcaccgg gattttcttt tatcgtctga ccgttgtccg
     6121 gtgccgtctc cgtcggttcc tctatgatgg tcttctgccc cggcacagct tccggtgctg
     6181 ttgccgaagg ctgtactggc atctgttctg cctcttccgg ggtcactgtc acatccacgc
     6241 caggccctgg cagaggtccc tcggtccgct gttgtcctgt taccgtctct ccggccgcat
     6301 cttcgaaagc caacagttcc agaacctcac tgtagattgt ggcggcctgc tctatgtctc
     6361 cgctctctac cagcccctgc agcatatttg tcagactttc tccggtctct gcgattcctg
     6421 ctgtcagctg atgtgtcaga ttgcgatagg atgccatctg tgtgatattt tcctccgtca
     6481 ccggaagttc cactttgtga agattcacga gatccataat ctccgcatcc gggaacgcat
     6541 tactttcatg ccagatctgc tgcaacgtat tcttatcaat aggaagcccc gcatccatca
     6601 gttgttttgt catggctacc gtattctcgt tcaccggcag cgatgccata tccaacgctt
     6661 tcagtacggt tccttctgtt gccatattgg taaaaagagg actcagattc agtgtctgcc
     6721 cgttattttt tacctgaaag gtaatgttct gccccagttc caggcggata tccgcatcca
     6781 cttttgcatc caccaggaca tcctgcagaa gacggatctg cgcattattg ccctccctgg
     6841 agaccacctc tcctctgagg gtctgcccgg gaaccagtgc acggatctgc cggtttacct
     6901 gttcggtatt cgcattattt ggagtctgta ctctgctgtt atccgctatt ttatcctgtg
     6961 agaaccatcc tgtcagcttc atattttctc tcccggcacg ctaaaatgcc ttaaaatccg
     7021 aaatttttga taaagctgtg tctgtggatc ggagtcggtc catactcctt cagtgccgca
     7081 atatgtgccg cactaccgta ccctttgttg gatgcaaaat catactccgg aaacatatcg
     7141 gcatactgct ccatcagacg gtccctggtc accttggcca caatactggc cgcaccgata
     7201 gaaatgctct tggcatcgcc cttgatgatc ggcacctgtc ggatctccac tccgggaatc
     7261 gtcacagcat cgttcaacaa cagatccggt gccggatcca gtttgccgac agcctcccgc
     7321 atggcctcat aggttgcctg taaaatattg atctcatcga tccgctccgg agaagcatag
     7381 cccactgccc aggccaccgc ttccttcgtg atcacatcgt acagctcttc tctctttttt
     7441 tcggataact gcttggaatc attaatgtat aaaatatcac aatccttcgg cagaatgaca
     7501 gcacctgcca ctaccggtcc cgccagagga cctcttccca cttcgtcaat tccgcagata
     7561 taggtatatt gtgcgtattc tttctcgtat tttttcagat tctcgatccg ctgacgttcc
     7621 ttttccagag catctatctt tttctgtgcg ctagacacca gcttctgcac accgcttctg
     7681 ggatcattct catattctgc tataaattca ggcagctttt ccattgaagc tgcctgtaat
     7741 atttccttga tctttgctgc actttccatt tgtttatccc catttatggt tattgatgtt
     7801 gtaatacacc gaaatcagaa accggccaga tccggagcca ggcttttccg atgatatttt
     7861 ctctttttat gttacccacc atatccgtac gactgtcgct ggaattgttg cggttgtctc
     7921 ccatgacaaa atactcatca tcccccagtt caatgggttc cgccgcccgg ccgatggtct
     7981 ccggttttat gacttccatg ccgtagttct cttccagctt ttccccgttg atgtagatac
     8041 tgccatcatc catgatctgt accgtctctc ccggcagtcc gatgatacgc ttgatataat
     8101 aggtgttgtc ctggaattga aacgggaaga caatgatgtc aaagcgttcc ggatcatgaa
     8161 aacgatagct taacttatcc acgatcagat tatccccgtt gtgcagggta ttctccatgg
     8221 aggctccctc cacttccgta cgctgtccta caaaggtgat caccagccat accgctccca
     8281 gtacacagag caggtagatc gctgtgttta ataattctct catgattctg ttcatctgtc
     8341 tatatcctct ccagtgttat tcgtcccagc ttgccgctcc ggaaatcatc gatcaggatg
     8401 ccggcggctt tccccagatc cagttgttct ccctttgcat agcatttgcg gctctctgcc
     8461 actgcttcca gtatctctat gggcttgccc gcaggatctg cctcgtagcg ttcctgcaaa
     8521 gccttcggat aaagctcttt gatggctccg atcagatcac aggccagttc atccggaatc
     8581 aggatctcat cgttcatgga tccgataaat gccagacgca ttcctacctg ctggtcctcg
     8641 aattttggcc acaggatacc gggggtatcc agaagttcca gtcctttatt cagacggatc
     8701 cactgttttc ccttggtcac accgggttta tttcccgtct tggcgcaggc ttttcccgcg
     8761 aaggaattga taaaagtgga ttttcccaca ttggggattc ccactaccat agcacgtacc
     8821 ggtcgattta cgatgcctcg ctttctgtca cgttcaatct tctccttgca gacttcctgt
     8881 accagcccct ggatcgactt gattcccata ccggtacggg aattgatttc cagtacaccc
     8941 attccctgat ttgaaaaata ctcgacccat ttcttattcc acactggatc tgccagatct
     9001 gatttattca acaaaatgat acgggattta ccttttccca gttcatcgat atccggattt
     9061 ctgctgctga tggggattct ggcatctaca agttcaatca ccagatcgat cagcttgata
     9121 ttttcctgca tcatacgcct tgctttggtc atatgaccgg gataccactg ataattcatt
     9181 tttctattct cctaccatac tttctatttg ataaagccga tgccatccgt cccggatttt
     9241 aagtggaacc aggctttgcc gatcatatcg ccctcctcca ccggtccgat attggcggat
     9301 ctgctgtctt cgctgttatt cgggttgtcc ccgatgcaga aatactcctt gttcccaagg
     9361 atgatctcgt tttccgctat acccgcatcc aggatccgtt ccgtcaccca tttgctttcc
     9421 tgtccgttga catacagagt tccgtccgcg atccttacct tatctcccgg ccctgccaca
     9481 acacgcttga cataataatg ggaattttca ttgccattcg gcagaaatgc tactatgtct
     9541 cccgccttcg gagaagataa aacatacaga aaacggtcaa taaatatctg ctgtccattc
     9601 tccagtgtcg gttccatgga catgcccaca actctggtgg actttcccag aaagataacc
     9661 accactgcag ccagaaagac tgacagtgca atgccgaaga tccagctgaa aatttccctg
     9721 agcagttcct gactgatctt tttctttttt ttataaaaac ttagtccctt atttctcaat
     9781 gttctgtcct atctcgcata gtgtttacaa gtctcagtgt accatatccg tattcctttt
     9841 tcaaataaat ataaaaggca gctgtcagaa acagctgccc ctctccggtc aatgtaatta
     9901 tttaactaat tccttaacct ttgccttctt accaacacga tctcttaagt aattcagctt
     9961 agcacgtctt actttacctt ttcttacaac ttcgatcttc tctacgttgg gagaatgtaa
    10021 aggccaagtc ttctctacgc cgataccgtt ggaagtcttt ctaacagtga aagtctctct
    10081 gttgctgccg ccctgtctct tcagtacaac gccttcgaat acctggattc tctcacggtt
    10141 accctctttg atcttgccgt ataccttaac ggtatcgccg acttcaaact gatcaacggt
    10201 tgcttttaac tgttctgctt cgatctcttt gataatgtta ctcataatga gcctccttca
    10261 taaaatggat gttcttaata cccttattgg tagcagagga ccatctcgtt ttcacaacat
    10321 tcagatttta ccataaagcc atataaaaag caagcttttt ttaattatac agccaccaga
    10381 tcacgggatc cgacagattc agccccaacc gttcctgcag ttgtatggac gcttcatttc
    10441 cattcacaat atgggcgtag gggatcatcc cctgttctct ctgtttgttg atcagataac
    10501 cttccaggga tgctgcaagt ccttgtctgc ggtatgcatc ctccacatac agcattccca
    10561 tgctgccgtc attgtggcat cctataaaac cacagagttt tccctcgata tagatgccga
    10621 agacatctcc tgctgtaatc ctctcccgga cataaatctc gtctcccagg tgataatgct
    10681 ccgccacata aggaacctct tccactgtca gctgccggat gtccttgtgc ctcaccggaa
    10741 gtggttcccc tctggtatag cacgcctggg aacatagcat actgccatga taccctaaaa
    10801 tttcactgac acgttcattc caccggtctg tcacggtcac gatactatga cccgacacgg
    10861 ctttcggaat gagcggcagc attttctcca aatgctcctc atcctccgca ttcagcatgg
    10921 cattgccgat ctccggcaga tagatcagta catttttccc ctccgcatag agaacttctc
    10981 ccaatcctct ggacagtgtc tccatcatat gaataaaaat acgtttcttc gcggaaagtt
    11041 tttttatgac tttctgtttt tcctgatatt ttgcatagag atccgggcgg cgttctcccg
    11101 tccgtctctc ggactgttcc agccgccagg cattgatctt tttatgattg cccgacagaa
    11161 gtacctccgg tactttcttt ccgtgccatt cctccgggcg ggaatactgg ggatattcca
    11221 gcagatcgtt atggaagctc tctgtctcgg cagattcgtc gttattgagt accccgggca
    11281 ccagtctggc caccgcgtcc accacgacca tggctgccag ttccccaccg gtgaggacat
    11341 aatcgccgat agaaatataa tccgtcacga cctcttccag aactctctca tcgattccct
    11401 cataatgtcc gcagagcaat accagttcct cttccccgga cagttctctt gcttttttct
    11461 gggtaaaagg agctccctgt ggtgtcagat agacgcagcg gatctttttt tctcctgcta
    11521 ccgaccggta ggcatcatag accggctgtg cctgcatcag cattccggcc ccgccgccat
    11581 aggtgtaatc atccactttt ccgtgcttat cctgtgtgta atcccggata ttcaccgcat
    11641 ctatggaaat cagatctttc tccgctgccc ggcctaaaat actggttgcc agcccctgct
    11701 gcaccatttc ggggaacagg gtcagaatat gaaacttcat cataagtcaa gcagtccttc
    11761 cagtacatgt acctgcattg tgccattttc cacgtccaca ttcaggatac actgtttgat
    11821 agcgggcaga agcaggctct taccgtctgc catttccacc tcgtagacat cattggcacc
    11881 ggtctcgatc acatctttta ccgtgcccag ttccttaccg tcttcatcct gcactttcag
    11941 tccgatcaga tcggcgatga aatactcgtc cctttgcaga cgcactgcat ttttgcgggt
    12001 cacgtacagg ctcttctgac gatatttttc tatatcatta atgttgtcca gccctttaaa
    12061 tttcaaaatc acaaactgct taaagaactt gacaccctca atttccagat tcattttttc
    12121 cttgccggta tccagaacga cttcctttaa tctgcggaat cttctgggat catccgtagt
    12181 aggatatacc tttacttctc ctctcacccc gtgagtggaa gtgataatgc cgacctgcaa
    12241 cagatcttcc atatagtttt actccattcc ggcttttagc caaaaaacgg agaaacacac
    12301 gcctgtatgt ttctccgctc aacgtcaggg atctctcaat tcgaaattaa acgatctcaa
    12361 cgtccactct tgtgttgtct ttggatgatg ctgctttcac aacggagcgg attgcttttg
    12421 caatacgtcc ctgcttaccg atcaccttac ccatatcgga cgcagctaca tgaagttcaa
    12481 ggacgatatt ttttccttct gtcttctcag ttacaactac ttcatccgga ttatcaacga
    12541 gtgcctttgc aactacttct actagttcct tcataatcca cctccgatga atagatattt
    12601 agaacttatt tctcgattcc tgcaaccttg aaaagcttag caacctgctc ggtaggctga
    12661 gcaccgttct gtaaccactt cttagccagc tcttcattga tcttgaaagt ggaaggatcg
    12721 gtggaaggat cgtaggtacc gatctcctca atgaatctac cgtctctggg ggaacgggaa
    12781 tctgctacga taattctgta aaaaggagcc ttcttctgtc ccattcttct taatctgatc
    12841 tttactgcca tttctttttc ctccattaat taaaaataaa ataatttata tgataagaac
    12901 tgattgttca tatcggactt aagttaagta aacagtgcta gaaaaatctg ccgccaatac
    12961 cgggaaattt tccgccgccg aacataccac ccatgccgcc gcggcccttt ttgccgccgc
    13021 caaactgctt catcatcttc tgtgtctgct cgaactgttt gataaaacgg ttcaccgttg
    13081 caatatccac accggcacct tttgcaatac ggtgttttct ctgaggggtc aggatcttgg
    13141 gattcctgcg ttcctctttt gtcatggata atacgatggc ttccgtacgc ttcatctgcg
    13201 tctcatcaat ggcaccttcc agatccgatg ccttgatccc cattccggga agcatcccta
    13261 agatgctgga cagaccgccc atattacgca tctgcttcat gctctccagg taatcctcaa
    13321 aatcgaactt acctttcttc atgcggccgg ccatatcacg gctcttctcc tcatcgatat
    13381 cgatgctggc ctgtgccttg tcaatgaggg ataacacatc acccatacca aggatacggc
    13441 ttgccatacg atcgggatag aactgctcta aatcggacaa tttctcgccc atacttacga
    13501 agaggatcgg cttgccggtg actgctttga tggaaagtgc cgcaccgcct ctggtatcac
    13561 cgtccatctt ggacaagatt actccatcga cgcctacctt ttcatcaaat tcttttgcca
    13621 catttacggc atcctgaccg gtcatggcat cgactaccaa aagtgtctga tgcacggtca
    13681 cggtctcctt gatctcctgt aattccgcca tcatgtcttc atccacatga agtcgtccgg
    13741 cagtatcgag gatcactaca ttatgaccgt tcttctccgc atatgcgatc gcattctgtg
    13801 cgatctctgc aggcttgtga tccgttccca tggagaacac atcgacttcc tgcttcttac
    13861 cgttgatctg taactgttca atggctgcag gacgataaat atcgcaggct accaataacg
    13921 gctttttgcc cttgagcttc agcttaccgg caattttcgc agttgctgtg gtcttacctg
    13981 caccctgaag acctgccatc atgatgacgg taatggcctt acccggctgc atggcaatct
    14041 ctgtggtctc actgcccaag agatttacca tatcttcatt aacgatcttg atcaccatca
    14101 gtccgggttg cagaccgttc atgccggcct ggccaatggc acgttcctcc actgccttca
    14161 cgaactgctt gactaccttg aagttaacat ctgcctccag gagcgccatc ttgacttcct
    14221 tcagtgcgga cttaacatcc tcttccgtca gacggccttt gcttcttaag tttttaaaaa
    14281 cattttggag tttgtctgtt aaactttcaa atgccatttg ctgctcctta ccattccttt
    14341 aaaagggatc tggacaattc ccggatgcgt ctcatccgtt cttcgggatc cgtggtatcc
    14401 tccgcagtca actgttcgat ctcaccgatc ttctccttgg ctgccatgaa gcgttccacc
    14461 agatgaagct tgctttcata atcctgcagg atcttatcgc aacgcttgat cagatcatgg
    14521 acgccctgtc tgctgatgcc ctgttctccc gcgatctcac tgagagacat atcattatat
    14581 actgcatcct catacactct ctgctgatgc tcggtgagaa gttcaccgta gaaatcatat
    14641 aacagattct gttccaaaat cctgtccatc ttatccagtg cctttctata ggtcatgcga
    14701 tatgcacctt ggctatcata ctatagaaa
//
LOCUS       DAWDHD010000056        14490 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_281_length_14490_cov_11.300727, whole genome shotgun sequence.
ACCESSION   DAWDHD010000056 DAWDHD010000000
VERSION     DAWDHD010000056.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 14490)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 14490)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14490
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_281_length_14490_cov_11.300727"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         4092 a         3083 c         3980 g         3335 t
ORIGIN      
        1 ggaatgagaa tatgagaaga tggaatttac cccaggtgaa tacaaaagta atgtgtgcag
       61 tgttgacagc tgctatggtg atgacgggat ttacggcatg cggaggaaca acatctgcgg
      121 gtacggagag cgtagcaagt gaatcagcag aacagaagga gacgatcgca ccggagagta
      181 cgacggagag cacggagagc ggtgaagaga aggtggaatc ttatctcggc cagatcactg
      241 ccattgatgg caatgtgatc tccatcgcac tggcagagaa gccggaagca cccaatggtg
      301 aagctcctac cggtgaggca cccagcggag aaaagccgga tggccaggct cctgacggca
      361 acggtggcgc tcccaatggt gaagccccta ccggcgaagc tcccagcgga gaaaagccga
      421 acggccagac tcctgatggc aatggcagcg cgcccaacgg agaagcaccg aacggtcaga
      481 aacccgagaa agacatgctg gatgagatga ccttaactct cagcggagat actatgacta
      541 tcacagtcaa tgacgatacg atcattacga ttgacggaga agatgccact ctggacgaac
      601 ttcaggtagg tgataccgta tcctttttcc tggatggtga gacagtgact tcattgagcg
      661 taggtatgcc ggagtcgaca cagcctatgc aggccccgga gcaaaattaa gctgacgtaa
      721 gagcatggca ccgagaatct ctgccatttc ttccggggac tggggatcgg attctttcag
      781 ccaggtctct acgacaccga cacagccggt gataatgaag gagcagagat attcataatt
      841 agcatcggat tgcgcacttt ccagaacctg gagagtgcgt ttttcaatct gatctttcag
      901 gcgattgacg aaagccagat ccccatgagg gccgatcatg accttggcca gatccctgtt
      961 tcggtccaga aaacggaaga attccaacaa cggcccctga gaggccagag aattggtaat
     1021 gaccggtttc tgctccatga tttcattaaa ttccaggaac atttcatcct cgattttatg
     1081 gagcatatcg tagatatcat cataatgcag ataaaaggtt ccgcggttga tatccaccag
     1141 ttcggtcagc tccttgacgg agatatcctg aatatcatgg gtttctaaca tttttaccag
     1201 cccctgtaat aagagagctc ttgtcttgcg cacacggcga tccatttttt ccataagaca
     1261 atccaccagc ctttccggga aaagaagaaa tcatttccta tatgtaatac aaatctgaat
     1321 tttttctaaa ataatagacg aacatcacga aaatgtctaa aaataaacaa tcacaaaaaa
     1381 tctgtagctt gctttttctt ccccattatt atattttgca tgtggcagaa aatcaacaca
     1441 agttcattaa atgacattaa tacaatttct tatctttgta gtaagtcttt acagatgttc
     1501 aaaaacattt taaactagag gtgtataaaa tcaaatacat aaacaagggt acaaagagag
     1561 aaaaagcagg agggaactat ggcagacaac gcagaaaaag acaaaaaaga taattccttt
     1621 atgatgaaac ttgccacgct cattgtagac aaacgaaaag gattttatct gatctttatt
     1681 gttttatgca tattttgcgt attatccatg aacagagtca aggttaataa cgatctgacc
     1741 acttatttgc cggacacaac agagaccaga aggggactgg atctgatgga ttcggaattt
     1801 accacgtatg gctccgcccg gatcctgatc tgtaacgtga cgtttgacga ggcacagaaa
     1861 ctggcagatc aggtggagga gatggacggt gtcagcatgt tggatttcga tgacaccgag
     1921 gatcattatc atgacatgga ggctttattg tctgtcactt ttgatgagga agcagacacg
     1981 gaagctacac agcagcatct ggatgaggta ttggatgcat tgtccgatta tgatgtttac
     2041 tattcttccg atatcggtca ggaagagcgt gacgcggatg acttgaataa tgatatgatc
     2101 gtcattctgt tgttggcagc agtggttatc gtagccgtat tgttatttac ctctacgact
     2161 tatgcggaaa tcccggttta tctgactaca tttattgtgg cagctatttt gaacaaaggt
     2221 acgaactact ggttcggaac catcagtttt gtaacgaact ctattgcggt ggtactgcag
     2281 ttaggtctgg cggtagacta tgccatcatt ctggcccacc gttttatgga agaacatgag
     2341 gacaaggatg ccagagaggc cgtgatcgtg gccctgagta aggcaatccc ggagatttct
     2401 tccagttccc tgaccactat ctccggtatg gtagccatga tgttcatgca gtttagaatt
     2461 ggttatgata tgggtatcat tctggcaaaa tccattattt tcagtatggt ggccgtattc
     2521 ttcctgatgc ccggactgct tttgaccttt tccaaggcca ttgacaatac tcaccacaag
     2581 agcttcgtgc cgaagatcac ggcggtcggt aaattctgtg tggctacccg ctacattata
     2641 ccgcctattc tgatcgtagg tgttattata gcatttttcc tgtccaataa ggcaaactac
     2701 gtgtacgata ccaacactct ggaatccagt actatgagtg ataataaatt ttccgtcagc
     2761 atggtaaata aggaattcgg catggtgaac cagctggcag tcattgtgcc caacggtgat
     2821 tatgaaaaag aggcacagac actgaaagct ctggaaaagc tggatgtggt gaaatcctgt
     2881 cagggacttg gcaatatcga ggccatggac ggctatatgc tgacggataa gctgaatccc
     2941 agacagtttg cggaactgat cgatctggat attgaagtgg cggatatgtt gtattcggca
     3001 tatgcactgg atcagagtga ttatggtgcg ctggtcagtg gcgtcagtga atacgaagtt
     3061 ccgtttattg atatgttcct gttcatttat gatcagaaag aaagcggcaa catcacactg
     3121 gacgatgatc tggaagaaac tctgacggat gcttattccc agatctgtat tgcgaaagat
     3181 cagctgctgg gcgaagacaa ttcccgattt gttctggaat tgaatgttcc ttatgaggga
     3241 gaagagacca cagcagcact ggatcagatc cgtaataccg tggcaaagtt ctataatata
     3301 gatgacattc tgctggtcgg taacgccacc agtgataagg atctgggcga ttccttcgca
     3361 acggataata cgatcatcac ggtgctgaca gcattgtttg ttatgatcat cctgctgttt
     3421 accttccagt ccgccggtct gccggtactg ttggttgtaa cgattcaggg tagtatctgg
     3481 atgaacttct cattgccgta tctgttgaat gaaaatgtat atttcctggg atatctggta
     3541 gtatctgcga tccagatggg tgcaacgatc gactacgcta tcgttatcac cagccgttac
     3601 atggatctga agacctatat gccgatcaag gaagccgcca tcgaagcact gaaccaggcg
     3661 ttccctacca ttgttaccag cggatccatg ctggtatctg cgggatttat tatcagctat
     3721 gtatcttcca atgcagcggt agcggccatc ggactggctc ttggtcgtgg tactctgaca
     3781 tctattttgt tggtactgct ggcattgccg cagacactgc tgatcggaga tatcattatt
     3841 cagaagacag catttacact gaaacgggat ctggcaaagc ccttgccctc cgccggaaga
     3901 atccgtatga acggtcacat caagggttat gtcaatggtg tgttggaggg tgatttcatc
     3961 ggtgtcattg atggtgagat gggtgcggtg gtgcgtccca aggataccgt gacgatggag
     4021 gagcgggacg atgccccgga agaaaactat cccagactgg aagcccagga gctgccggag
     4081 gatgagacag ccggacagaa agcaaccgga gcagatacag aagctgcgga taagaaagct
     4141 tcggataaag aaaaaacagc agaaagcgag ggaacagaag atgaacagta agagattatt
     4201 atgtttcctg ctgggagcag cactgatcct gcagacaccg gccaccgcct atgcagcaga
     4261 aactttgacc tatgagcagt acagaggcgg cagcggttat agcagcacga taaaggagca
     4321 ggattatgca gtcatagaga tcagcacgga ggaggatctg cggaaactgg tggagaactg
     4381 tgtactggac agttggtcca gagataaaaa agtagtgctg cagaacgaca ttgttctgag
     4441 tatgacaggt gaactcagta ttcctacgtt tgcaggaatc tttgacggca gcggttttac
     4501 tatttccaat gtgaaactga ccggagacgg ttcggcagtg ggattgttcc gctatgtgca
     4561 ggagggagca aaagtccgca accttacggt cacgggggaa gtatctccct ctggaagtca
     4621 ggatcaggtc ggcggtatcg tcggtgtgaa ctatggcagc attgaaaatt gtaaatttac
     4681 cggaaatgtg gtcggtgata cggatgtcgg cggtattgcc ggagtcaatg ccgagagcgg
     4741 agagatccgt cggtgtgaat cctccggaaa tgtgatcggc aatcattccg caggcggtat
     4801 tgtgggcaac aaccacggta ttctgaataa ctgcagcaac aatggcaata tcaatacgta
     4861 cagcacggaa gtgacttatg atctggaaga tatcaccatg gacaatctgg agcagatcaa
     4921 cagtacctcc aatgtggcag ctcatacgga caccggcggt atcgcaggta tttcggatgg
     4981 taagatttat tattgctcca attccggtgc catcggctac cagcacgtag gctacaatac
     5041 cggaggtatc gtaggcagac tgcatcaggg ctatctgcag aactgcacca acactggata
     5101 tgtgcagggc agaaaagatg tgggcggtat cgtgggtcag atggagccgt tcctggaaat
     5161 ccagtatctc agtgacaagc tgaaggaact ggatacggag acagacaaat tcctggatat
     5221 gttggatgcc acccagaaag atgtgagtag ctatagcaaa caggcatcca gtattgccaa
     5281 gagtatcagt tcgaacctga aagatgccaa caatgcggga agttctctga ccgggaccgc
     5341 tcatgatctg tggtatattt acaatcaaga gttgaacggc gtcagcaacg atctgaaagt
     5401 cctgaatgac gatctgaaca agcaggcgga taatgacaag aataacggca acagtcatga
     5461 cgttaccatc agtggaaatg acatttggaa tggtaactgg ggtggcgatg gcgatcatga
     5521 cgtcagtggc ggaaacatta ccatcaccgt accggacgat acggagagct ataaatcggc
     5581 actgaaaaaa ttcggagaga atgccaccaa gcgtctggat aacatgacga atgcttccac
     5641 ggatcgcagt ggcggtatca aagataatct ggatacactg aatgacaggc tcgagaaggc
     5701 ttttgaccag ttgggtcagc tgggggatgt actggaagcc gggacagaca gcaccagtgc
     5761 ccacatggat gagctgatgg atcaggccag agttctgcgg aatctggtca gcgaaatccg
     5821 ggatgatctc ttccggtatg aaggaatttc tgtagaagat acttcggatg agtcagccag
     5881 caaggaagag gtgaaccccg gagatcccga tgcagaggat gacaccgcag aaccggaagg
     5941 aaccgaagaa gcgcgctatg atacttccag cttccagaag ggaaaggtca ccttatgtgt
     6001 gaaccgcggt acggtggaag ccgatacgaa tgtgggcggt atcgttggac aggtggcaac
     6061 ggaatacgat tttgaccctg aggacgatat cactttaacc ggaacggaga gtttcgatgt
     6121 ggagcagacg gtcaaggcag tcatcagaga cagccgcaac cttggagacg tgaccggtaa
     6181 aaaagactat atcggcggtg tggttggtaa agcagaatat ggcgcagtca tttcctgtga
     6241 gtcttatgca cccgtagaga gtaccggagg cagctatgtg ggaggaattg ccggatctgc
     6301 cagctatgcg atccgcagct gttacagcat gggcaggatc accggtaaaa ataatatcgg
     6361 aggtattgcc ggagaaggct gtgatatttt ctacagctat gcgtacaatg atctggatat
     6421 gtccggcgaa ggccagggca gcattgccgg aaaagtcagt gatgatggaa ctctgtatgg
     6481 caactattat gtggaaggcg gagccggtgg cgtagacggc attggttatc agggcggtgc
     6541 taccccgttg tcctaccagg aattctgcag caaagacgtt ccggacgctt tctcacagtt
     6601 taccattacc ttccaggcgg atggtgtaga agtggcttcc tataaatgcg gttacgggga
     6661 ttatctgtcg gcagaccaga ttccggaagt accggagaag gatggctatt acggtgtatg
     6721 gccggattat gatttcagtg atattaccgg taataaagtg ctggaagcag agtatgagga
     6781 atggaccgca tccattgctt ccgcagagaa aaacgataac aataaggcac tggttatggc
     6841 agaaggcaac ttctatccca atgcagcatt gcatctgcag gtagagggaa atacttatac
     6901 agtatccatg accaattcca tggaagagga tgcaccggac tataccggag aagcaacctt
     6961 gcgtgtctac tgtgaagatg cggataacac tgtcatagag gtagcacagg atggagagta
     7021 cacggaagtg gaatccactg tgatcggcag ctatagacag tttaccatgg aagtgcccgg
     7081 cagcttccgc acggtagagg tcgagggcag tcatacactg ctgatcgtac tttgcatcgt
     7141 cggtggtgca gtcgtgatcc tgttgatcgt attgttaggg aagaaagcgg caaaacgccg
     7201 gaagacgcgc aaggcggcaa aacgtgaccg gaaagccgga aaagctgatg aggaccagag
     7261 cggcacaaac gaaagtgatt caaaggacag cccagcagaa ggtactgatg ttacggatgc
     7321 agcagaagat gccggacaga cggctgacgc agaggaataa caatgattca ggcagtttta
     7381 tttgacatgg acggtacgct gatcgatacc gaaaaatatt accgtatttt ctggccgaag
     7441 gcactggcag cctgtgggta taccatgaca gatgagcagg cacttacgat gcgcagtctg
     7501 ggacgcccct ttgcccccag acagctcaag gactggtacg gtgaagattt tgactattat
     7561 atggtgcgtg acaaacgaaa agagatgatg gaagaaacac tggaccggga gggcatcaaa
     7621 tgcaagcccg gtgccgtgga gcttctggaa gatctgaaaa aaaggcatat caccgcagcg
     7681 gttgctacag cgacggatct ggagcggacg gaaaaatatc tggcgatggc aggacttcgg
     7741 ccgtattttg agaaactgat ttcggccact atggtagcag aaggtaagcc gtccccggat
     7801 atttatctct atgcctgcaa gcagctggga ctggcaccgg aagagtgcat ggccgtggag
     7861 gattctccca acggagtcat gagtgcttac cgcgccggct gtaaggtcgt gatggtgccg
     7921 gatcagacac agccagatga aaaattaaaa aaatgtctgt acgcctgcgt accgaccctg
     7981 gcagatatca gccacctcat ttagaggtgg ctatatttgt catagtagct tgatttttgt
     8041 attccagtgt gttattattt taacgattag agtgactatt cggaagacaa tgaaatcaag
     8101 cgggactgct ttgtcatgct tgcatgtaaa aagcagtttt gcttgatttt aggtgactcg
     8161 aatagagtca ccgccacaca tatgagcaaa aatagctgcg tagcagcgaa agcacgattt
     8221 atcgtgcgat tttgcgaata atgtgtggaa tgtattcata cttggagccc acgaggggcg
     8281 gaaaggaatg gaagaaaatg aattttgatg acatcgtagc aaaagtaaaa gagtgtggga
     8341 agaagaccgt cgcagtctcc gttgcacagg acagtgcagt actggaagct gtacatgagg
     8401 ccaagagact gggcattgca gatgcaatcc tggtaggtga tgaggcaaag atccgtgaga
     8461 ttgcagccgg actgaatatg gacctctccg attatgaaat catcaatgag ccggatatga
     8521 tcgaagcttc cctgaaagct gtaaagctgg cacatgacgg ccgtgcagat atgtatatga
     8581 agggcctgat cgactccaag aacttcttaa agagcgtact gaacaaagaa gtaggccttc
     8641 gtaccggtgg aaccctgtct catgtatgtg tatttgagat tcccggcatt gacagacttc
     8701 tgttcctgac cgatgttgca ttcatgacct atcctacact ggaggagaag gttcagatca
     8761 tcaagaacac cattccggta tgtaatgcct gcggcgttgc agagcctaag gtagcacctt
     8821 tagcagcagt tgaggttgta aatcccaaga tgcccgttac cgtagatgcg gcagaactga
     8881 ctaagatgtg tgaagaagga cagatccccg gctgtatcgt agacggcccg ctgtctcttg
     8941 acctggcgat cgatcccgag gcagccaagc ataagggagc taccgaccgt aagatccagg
     9001 gtgatgcaga tgtattgctg ttcccggata tccatgccgg caacctggta tacaaagcaa
     9061 tcgtacatat ggtaaaagta aagaacggat gcatcctgac cggtaccaag gtacccgtag
     9121 tcctgaccag ccgttccgat accttcgaga ccaaggtata ctccctggca ctggctgcag
     9181 ttgtagcaga agagatgaaa aagaatcaga aataagctat gaatatcgtg atcgaagccg
     9241 ctgtggaagc tgcgggagaa ctgctgtcgg caggtataga tgctgtagtg gacaaagcgg
     9301 taaagcataa gagcgaatat aagagtgaat ataggaggaa acacacaatg gcaatcaaga
     9361 gtctgatcat caaccccggt tccacctcca ccaagatcgg tgtatttgag gatgagaact
     9421 tattatttga agaaaccctg agacattcta ccgaggagat ctccaaatac gatactattt
     9481 tcgaacagaa ggatttccgt aagaagatca ttaccgatct gctggcagag aagaactgcg
     9541 atctgaagag cttcaacgtg atcgtaggac gtggcggtct gttaaagccc atccccagtg
     9601 gtacctatgc ggtaaccgat gatctgctgg aggatttaaa agtaggtgta cagggtcagc
     9661 atgcttctaa cttaggcggt attctggcaa gagagatcgg tgatgagatc ggcgttccct
     9721 cttacatcgt agaccccgtt gtagtagatg agctgatgcc catcgcaaga tattccggtc
     9781 tggaagagat ccccagagga agtatcttcc atgccctgaa ccagaaggca gttgccaaga
     9841 gatatgcaaa agaggccggc aagccttatg agtccttacg tctggtagta gtgcacatgg
     9901 gcggcggtgt atccgtaggc gcacatgagg acggcaaggt tgtagacgta ttcagtgcat
     9961 ttgacggtga tggtgcattc tctccggagc gtgcaggcgg tgtgccttgt gcagcactgg
    10021 taaaaatgtg cttctccgga aaatataccg agaaggagat cagtgcaaag ctgatcggca
    10081 agggcggact gaattcttac ctgggtacca acgatatgcg tgaagttacc aagcgtgcca
    10141 atgaaggcga tgccaaggca gcggaggtga agcaggcatt cctgttacag gtagccaaag
    10201 acatcggtgc tatggcctgt gtgctgaacg gcaaggtaga ccagattgtt atcaccggag
    10261 gcattgcata tggtgaggat gtagtggcta agctgaaaga gagatgcggc tggatcgctc
    10321 ctgtgaccgt atatcccggt gaggacgagc tgttagctct ggctcagggc gctctgcgtg
    10381 tcatgaacgg cgaagaacag gtgaagcagt actaatttag agtaaaaata tagaactccc
    10441 ggtggaatga ttccatcggg agttttttac tttagggaat agactttctg tcagcattat
    10501 gattttaact aaaaatcagc tttgacagaa tccccagtac cagcatgtgt accggataaa
    10561 aggcatagca ggcaagctgg atcttacggt tatgcgcgcc ctggcgtcca tggtagcacc
    10621 acagaagcgg cagacacaac agcgccagtc cctgttctga aatctcgaag gcatgaccga
    10681 aaagctgtaa tggaatctgc attcctgcca acagcatgac attgatccag tagagcccgg
    10741 caaactgtcc ggcaagctgc caccaatttt tcccatggaa gagatagaat acgataacag
    10801 tcagaacgcc ttctccgtaa tagtcgaaca tgaggagggt tgccagcacg tagcccactg
    10861 cagatagcag caggatcgac ggaatccaca gccagaccgg acattttttc cgtagggtat
    10921 ctattgccca gatacacagg aagcctgtga gcagtgtcca gatcacgttc tgatgaaacg
    10981 ggtagaataa cagcccactg tacatcagat tgatgggaag ctcagaaaga atggccagaa
    11041 gaagcaggcg catgcaatat tttttcctat catgggtatg gaagaagcct tccaccagaa
    11101 aaaaggcgaa catgggaaat gccagtctgc cgatgccggt cagccaccag gcatctccgg
    11161 cgagggtagc ccacaggtga tcacagaaca tgaacgccat agcgaggata tgcagcgtca
    11221 gagcactgag atccggtggg aaaatagaac aatgggatac tttgttttgc aaaataaaac
    11281 tcctttcaaa attccttacg gaaggatata gttgcattat atcttgttta tgcgctatag
    11341 taaatatatc tgtgaaagag tatatgaaat tccaatgatg gcttacccgg catttatatg
    11401 ccgcaaagcc tgtcgggcgc acagatattg acagaaaaac aaaatgcagc acaggaggaa
    11461 tacaatgcgt cgtaacgatc gtgagattac agaattttcg gaaatgacag atgtcatgaa
    11521 gaggtgtgat gtgtgcagac ttgcactgaa tgatgaggaa ggctatccgt atcttctgcc
    11581 gttgaatttc ggaatggaag agacggacgg cagagtgaca ctctattttc acggggcaaa
    11641 agaaggaaaa aaatatgact tgatccgtaa ggatgtcagg gcaagcttcg agatggattg
    11701 ctcccacaga ctggtgctgt cggagaaaga agatggctgc cagtgcacca tggaatacga
    11761 gagcgtgatc ggtcagggaa ctatagagat cctgtcggat gcggaaaaac agaaagcatt
    11821 gaatcttctg atgaagcagt actatcctga cagaacggag aatttcgtat tccgaccgga
    11881 agtcgtagcc atgacggaga tattctgcct gaaagtggag catatgacgg ggaagaaacg
    11941 tttcaaaaag tcgtagaaga tatcttctat tgatatcccc acgggatagg gcagcaggaa
    12001 aatagagata aaatagagag gatgagggac aattatgtat acagacggac agatctatat
    12061 gggcctggca gatggtcagc gtgtgaatat gtcgctgaat atgagtaacc gccatggact
    12121 gatcgcagga gccagcggca ccggcaagac gatcactatg aaggctatgg cagaatcttt
    12181 ttcagatgcg ggagtgccgg tatttttgtg tgatgtgaag ggggatgtct ccggaattgc
    12241 cacagccggt gtggcttcgg aatccatgga caggaggatt gataaattcg gtatccgcaa
    12301 cagtttttcc tataaggctt atccggtctg cttctgggac atctatcagg agggcggcca
    12361 tccggtgaga gccaccatct ccgatatggg accggagctt ttatctcgta ttctggggct
    12421 caccgctgcg caggaaggtg tattgaatat tgtcttccgg atcgcggatg acaagggact
    12481 tttattgatc gacttaaagg atctgcgtat tctgctgaat tatgtggcag aacacaagga
    12541 tgactatctg accacctacg gaagtatctc caggcagtcc gtgggcggta ttctgcgggc
    12601 gttactgcct ctggagaatc agggcggaga tctttttttc ggagaaccgg atctggatat
    12661 ttatgactgg atgcgtacgg atgtgtacgg caaaggtatt gtcaatgtgc tgaactgcgt
    12721 aaaactggtg cagaatccca cactgtatgc cagctttctg ttatggatga tgtcagaatt
    12781 attccagaag cttcccgagg cgggagatct ggaaaagccg aagctggtat ttttcttcga
    12841 tgaggcacac ctgttgtttg ctgatgcacc gaagatactg gtgcagaaga tcgagcaggt
    12901 ggtaaagctg atccgttcca agggcgtggg tatctatttc gtgacccaga gccccagtga
    12961 tattcccgat tccgtgctgg cacagttaag caaccgggta cagcatgccc tccgggcgta
    13021 caccccggcg gagcagaaag ctgtccgggc agcagcacag tccctgcggg ccaatcctgc
    13081 ctttaaggca gaagaagtca tcatggaact gggtgtaggt gaggcactga cttcctttct
    13141 ggatgagaac ggcgtgcccg gcattgccca gcgtacctcc atcatctgcc cccagagctt
    13201 tatgggacct gtggatgaga cggagcgcag gaatctgatg gcgagagacg gcatgggacg
    13261 ctacgatgag gcgatggaca atatttctgc ctacgaagtg ttgacagata ccatggagca
    13321 tgaggcgaaa gcggcgcagc tggcagcaga gcgggcacag ttggaaaaag ataaggaagc
    13381 gctggcaaag cagcaggcca agctacagga atccaccgta aagcagaagc agaaggaatg
    13441 ggaagccgca cagaaaaaga aggaaaaggc agcggaacgc agacaggccc agatcgagcg
    13501 tcagctgatt aacaccggtg cccaggtcat caagagagga attttgagta cactgtttgg
    13561 aaagtaatta ggaataaaat tcttttgatg cttattctgc catcgtcagc ggacggaaag
    13621 attatgcaaa acgatggaaa ggctagaata actgcaacca gagaaagtgt ccgccaaaga
    13681 tcagccagag gacggacaga accgacagca ggatacagac gatggtcttc gccggctgcc
    13741 gggggcgtct gcaaagtaac ggcggtagag cgatggcaag tagaaggatc ggaatcccgt
    13801 cccggaaaag cataagcccg atggcaaagg cacccaaagc cagggtgaag agtgcttcgt
    13861 ctaaatttgc ttcattttgt ataaataaga acgcaccagt aaagaagaga aacgcaagaa
    13921 ggatcacgga aaaaatacta agacagagga tgcgaagcca tttcgcggct ctgtcttttt
    13981 tctgttgtct tacctcggtg atgacatcgc tcaggagtgt attggtgtca gcagagggca
    14041 gggcaatccg ctcaccgcga caaagctctt caaccgatac cgacagcaca gccgccagat
    14101 cgtccaacag agaaatgtcg gggtaagcat tgccggtttc ccaacgtgag attgccttgt
    14161 tcgtaatatg taactgttcg gccagctgtt gctgcgtcat tccaagagcc atccgctgtt
    14221 ccttgatgaa agttcccatt ttggccttat ccatgtgctt caccagcccc ctttatagtt
    14281 ttaatatttg atttcagtat agaagaatcc gggggaattg gcaatctacc gaagcgagaa
    14341 tgccgggaaa ttaactggaa agttacgcga gcaatgagcc gcgcataggc atagtgaaga
    14401 cagcgatgag cgcttgcgca cgattcgctg tcttcactta tgccgagatg cggcgaacgc
    14461 cgcgatatga aataagattg gcagcgtttt
//
LOCUS       DAWDHD010000057        14372 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_284_length_14372_cov_12.216805, whole genome shotgun sequence.
ACCESSION   DAWDHD010000057 DAWDHD010000000
VERSION     DAWDHD010000057.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 14372)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 14372)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14372
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_284_length_14372_cov_12.216805"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         4356 a         2751 c         3720 g         3545 t
ORIGIN      
        1 caaattctga aaatacaggt ggggcgaggg tagaaatatt tttcctgagt agtcaggaaa
       61 caagctaaat agaccgcaat taagctatgt aaaagaaatt ttacatgaga gccagcggtc
      121 tggcagagat agccaaagta cttcagacta cgccgaatta tctgctgggt tttgcagata
      181 ataatgatgg atttgcggat gaagcgcttg gtttgttgcg agatgtgaag gatcagtctg
      241 taagagagat tcttttgaag cagatcaggg cactgatttg aaaaaatgta ttgcctttgt
      301 ccgtacattg acttataata tgggtaaaag aaaggggcgt gatgatatgg cagctaaatc
      361 agcaaatctt tatgcaagaa tagagccgga tgttaaagaa caggctgagg gaattctggc
      421 tactttgggg atcccggcat ctaatgctat caatatgttt tataagcaga tcattctaaa
      481 tagggggctt ccgtttgagg taaagattcc aactgcgaga cctgttgata tttcgagaat
      541 gaacgtagag acgttggaca tggaacttga gaaaggatat gcggatatgc aggcagggcg
      601 taccaaatct gcagctcagg tttttgcgga tatccgaagg gattataatg tatgaaatat
      661 gatgttacct ttacggagca ggctgagaac gatcttcgcg gtatctttga gtatatagca
      721 attgatcttc tttcgcctga gaatgcagcc ggacagcttg accggatcga agaaaaaatc
      781 ctttccttgg gagagtatcc ggagaagttc cgacaatatg aaagagagcc ctggaagagt
      841 cgaaatacac gagttgtgcc tgttgataat tatgctattt tctatatccc cgataagaca
      901 aagaaattgg ttacagtgat tcgggttatg tattccggaa gagatgtaga taagcagctt
      961 gatgaattta caaagttata atcataaatc ctatgaggcg tatgagacat ttggtttcat
     1021 acgtcttttc ttttttactt ttttgagttg actaccggtc aacagaacag tcccttattt
     1081 cagggaaaca ttataaggag tgaatacttt ctgtaccagg ctttgcgaga acgaaacgaa
     1141 tctcaaggtc attcagacgg tgatgggaca caaggacatc ccagtaccac acgctaagac
     1201 attagcacgg aacaggacgg caatcctcaa agaccctgaa atgcctttgg acggagccaa
     1261 ggggacaaag acggaatagg acgtaagaac tgaccaatcc catcccgaca atgaagagct
     1321 tagacagtag cgcttccaag aaagctgacg gcaagtcaga aggtgctcag accgccggtg
     1381 ataacaacgg attcttcact ccgaacagca agatcgattt ctccaacgtg aagattgagc
     1441 ccctgttcga ggaagccgta gacttcgaga ctttcagcaa gtccgatttc agagctgtta
     1501 aggtaaaaga gtgcgtggca gtacctaagt ccaagaagct gttacagttc actctggatg
     1561 acggcaccgg caccgaccgc accatcctct ccggtatcca cgcttactat gagccggaag
     1621 aactggtagg caagaccctg atcgccatca ccaatctgcc ccccagagcc atgatgggca
     1681 ttgagtcctg tggtatgctg ctgtccgcag tatgcgagga gaacggcgaa gagaagctga
     1741 acctgctcat ggtggacaac catattccgg caggcgcgaa gctgtactaa gagaaaaata
     1801 agatagaacg cccccccatc ggaccgtttc ctcggtctga tgggggtttt tgaataaaag
     1861 ggataatatc agaagcataa ggtattattt tgagaagtaa agatatctac agcggaagac
     1921 aataaggaca aagttaaaaa tcaaccaccg gtagaatgac attattctcc gaatctggta
     1981 tactttcagt aaaaacagga ggtatccagg atggaaaagg caaaagtaac gatgagaaac
     2041 tgggagccct atgtatatga cggagagtac aatctgtcgg ggactgcgga tgtacatcca
     2101 cggttgggaa gaaatgtcta tgtagccacg acatccacgt tggtcaaggc ttctctggaa
     2161 gaggatgttt tgatctatga gaccagaaat acggtctatc actgcccgct gaaatatatg
     2221 atggtatcac cgtatgggaa tgtagtacag gaataccggg aggaactggc ccgtcttgat
     2281 acttcggaaa atgcactgga ccggatcatt gccgcagcgg cgaaaatgtc acttggagag
     2341 ccggaggata cggcggatga aatggtgaga aagatccgtg ctctgcagga gacaggacag
     2401 caggagattg cgcagatgga agaacaggag aagcagcgcc tgctcgaaat tgccgggaaa
     2461 tatgaggact gtgtctacat tgaagtgtcc agcgttcaca gcggaagcaa attggtatat
     2521 catctcgggg atgctgtggg aattgtgaat ccgggagttc acatcggaat gtttcaggac
     2581 agtgtattat acatgaaata tgccaccgaa gaagatccct gtgcattgga tttccggtat
     2641 tttccgaagg gattcggtga tgtcatggag acctacagct ggtccgataa tattaagcag
     2701 gccgtgatca aaaatcaaaa gggatacagc ctgatcttca accatgaaga aatcgctccc
     2761 ggagagacca aggtatttac cccggatacc cataagcagg gactgttcag cccggactgc
     2821 tataatggga aatctttgtt tacaatgggg gaggaagatg cggagaagga agactgagag
     2881 cgcagagtat ggtaaaaaat tccaaagaaa aggaaaatgt aaacaatttt caagaaatac
     2941 aggcatgctg cccggcctgc aatgcatttg cggataaaat agaatgataa gaaggagatg
     3001 agagccagcg gtctggcaga gatagccaaa gtacttcaga ctaggctgat tgcactatga
     3061 tgcgtcgaag ttataccaca agtataatga caaactaatg ttgctgtggt attatgtcga
     3121 taacaaagaa agaaaagcgg atccgaagcg atgggtctgg gaaaggaaaa aagaatgttc
     3181 acagctattc aatcaagtta tttatatctg gaaagtttga aaaatgcagc actcgattat
     3241 gaaaaagagt ggggctttag ctttgtgtat tcagataaga ataagcttga aggaaatgct
     3301 gtggtgatgg atgtaggcct gtcgtaggga caggagagga caaccggata catagatttt
     3361 gaaccgctta tcttgtgtgg atgcacatgg taggcggtat ttttttgcag atataagtct
     3421 gattggtaag gcagttctgc gttatgctcc gttagctcaa tggtagagca cctgactttt
     3481 aatcaggtgg ttaggggttc gagtcccccg cggggcactg tccaatgtaa atatcacaga
     3541 aatgaggtaa gagaagcatg gatttaattg aaatcaaagg aaaaataaat acagccatct
     3601 gctatgcaag agtagtagag gatgaagcga tagaacaaat cagacgtatg tgtgattatc
     3661 cgatgacgga aggatcaaga ataagaatca tgcctgatgt tcattccggt aaaggctgca
     3721 ctattggaac cacaatgaca attactgata aggctgttcc aaatgtagtg ggtgttgata
     3781 tcggatgcgg tatgtatacc gtgaacttag gcaaggttga tattgatttt gtaaaggttg
     3841 atgaggctgc acattttatt ccatcgggaa tgaacgtgtg ggaaggtcgc caggagagat
     3901 ttgatttgac agaacttgct tgctacagag aactgaagga cacaaagaga ttagagcgtt
     3961 cacttggaac ccttggtggt ggtaaccact ttatagagat agatgaagcg ggtgatggaa
     4021 ccaaatatct tgtgattcat tcaggctcac gtaatctggg taagcaggtt gcagagcttt
     4081 atcagaagct ggcgattaac ctggacagag gctatgggga ctatcttgag aagcgtgatg
     4141 agattatcag aacctataag gagcagggac gtagaagtga gattcaggat gcgttaaaac
     4201 agcttcactg gcaggtatat gagtctgaga caagtatgcc tgaagatctg tgctacttat
     4261 caggaaaata tcttgaggac taccttcacg atgttgaaat ctgccaggct tttgcaagac
     4321 gaagcagaga gaaaatggct gagattattc tggagagaac cgggatggct ggcagagaag
     4381 ccttccatac aattcataat tatattgata cagatgaaat gattttgaga aagggcgcaa
     4441 tagcagccca tagtggagag aaagtgctga ttccaataaa tatgagagat ggaagtgtgc
     4501 ttgctgttgg aaaaggaaat ccggagtgga attattctgc acctcacgga gcaggacgac
     4561 ttatgtccag aacaaaggcg aaagcaaatc ttagcatgga tgaatacaga gagacaatga
     4621 agggtattta taccacttcg attaatgaaa atacattgga cgaggcaccg atggcataca
     4681 agagtctgga ggacatcatt gatgtgattc gagagtctgt agatgtaatc gatgtgatga
     4741 aacctatata caattttaag gcttctgatt aaggtggagg gcattcttcc ttcacctgca
     4801 gaagcagaaa cggaggaaga aatgtttact atgcctacca tcgatatggt agctaccgga
     4861 aggaatatca tgagactgag agaggtagca ggtctgactg taagagacct gcaggatatc
     4921 tttggatttg cgactcctca ggccatctac aagtggcagc atggaacggc catgcctaca
     4981 atcgataatc tggttgtact ggcagcagtg ctggatgttc cgatggatga gattatcgta
     5041 atcgatgtaa atagaacaaa gcagattagc gcatgaaaag tgaataaaca gatagccctt
     5101 tcaaggctaa aatgttcggt tcaagtccgg cgtggatcac ttatgggtag gtcgcatagt
     5161 ggcaattgca gcggactgta aatccgccga ctttaagtct acgccagttc gagtctggcc
     5221 ctgcccactt atgtttgcgt gtccgagttg gtttaaggtg ccactggcac atagcgccct
     5281 tccgcaaacg ctattatatt taacaggtga ttattatggg aaaggtatat ttaaaaacaa
     5341 aatatgacaa tgaaatgaaa atggagatac ctcacaatca taattttgct tcagctatgt
     5401 ttggtttcag agagatggct gcggaaatag ttccttacca tttgatagat gatatctatg
     5461 atgattttaa gcgtgaagat attgccttgg attatattga tcagtgcaat gtactgttta
     5521 acaagtttgg ggtgacaccg catattcctg attatccaga tgtgcttaag ccatttctgg
     5581 gaaggaaaat atggaaagat acaatcaaca gtatttcaag agatgagaac aagtggccgg
     5641 cgggatattt tgtgaaacca gcagtaagaa gcaaggcttt taccggcaag acaatttcca
     5701 gtataaaaga tcttatgggc tgcggtaatc atgctgaaga ttatgaggta ttggtaagcg
     5761 aatcattaga tatcgctgca gaatggcgtt gctttattac atatgatgag ataattgatg
     5821 tgcgcccata tggcatgata attgataagt caaggaaggg ttatttatat cattatgacg
     5881 cgcaggtgct taattctatg atggaaagtt ttgtttcttg ggaagatagg cctatggctt
     5941 gcagtatgga tatctgtgta actaaagatg gcaggacatt actcgtagag ttcaacgatg
     6001 cgtattccct gggggcatat ggattggcag atatatatta tgctaagctg atttctgcaa
     6061 gatggagcca gttgttaggt gttaaggatg agtatcattt ttaagataaa agatgaatac
     6121 gatagcaatt aaactattgg ttaaatgcaa gtatgaggag attctgttaa gcttaatctt
     6181 tagaaggaaa actataaacc cgtccgcact gaaagaagtg gaagtctgcg agttgctgta
     6241 aatgacccgg caggacgtta aagtaaggct gcaatctatt aatctcatag agtagagcag
     6301 gttggtgaga ttatattgcc atagggaata tgattaagcg ttagtaacca taaagagctc
     6361 acggcagcga tatccgggaa acaagacgga ctccactaat cccctctata cataaattgc
     6421 gaatcctgat tattacgagc ctatggcatg taaaaaaact gtagcaatga gaacttgcaa
     6481 ggtactgtaa cagggagtga gtatagggaa gctcgcacag acattttaaa ctaaaagttt
     6541 aatgcatttt gtgaatacat ccgatataat gtaagtctac aaatagagat tgtgattcgg
     6601 gatggaagaa aattacggag gtacagagag atggaaccaa tgtatttgtc aatccagcaa
     6661 aaggagacag ggaagcaaat taagaaactg ctcatggaaa atggttatac tgtgaaagat
     6721 gtgcagaatg ctatgggatt tgagaatccg caggcaatct acaaatggat ttccggtaga
     6781 tcattaccaa gcttagacaa ttttgtaatc ttaagcagat tactgcatac cagtattgaa
     6841 gatatcctcg tcgttgacgg ggatgttgtt cgtttatggg gaattattca tgactataga
     6901 aaatctgact gcggattttc tatggtatta aaccgttggt acaatgacaa agcatctgtc
     6961 atgcagtaag ctaatatcag aacaaaaaac aaaacaaaat ggaggtcgta aggaaatgca
     7021 gacaatgaca tcagcatatg caaataaaat gcttaagagt ctggaagaag ataaggcctt
     7081 ctgggtaaac aaagaggcaa catccagtac ctatgttgca gctatcaatg aggaacctgt
     7141 cgtaccagaa tatgattata cggaggttgt agctactatt acatctcttg atgagaaaat
     7201 tgcaattatc aagcatgcgc ttaatgtaac caattctaat gaaaaggttc gggtgggcga
     7261 tgcagaaatg agcattgata caattcttat aaagatggct cagctgaaca agagaaaatc
     7321 agtgcttgat cagatgcgta agcagctacc aaaagccaga gaggagcagc gctcttatat
     7381 gtccagaaat acggttccgg aatataggta tattaactat gacctggagc tgattaagaa
     7441 ggagtatgag actgtatcca agaccattat ggaaatgcag atggctcttg ataagtacaa
     7501 tcaaacagta cagtttgaag tagacatcta agaatttttc cgtacaaggc tttatgagat
     7561 tgatggtgat cccagaagtt tttgtttatg gtaagggtat gtctgttatg ggttatgtgt
     7621 tatcggttta tgttcattgt aggttttaaa atctgatgca agatttctta acagcggcct
     7681 tgtagaaaac ctgcaggaaa ctgcgtgtgg aggttcggtt ataagcacag gctgtgggag
     7741 gtcttcggat cttctgcagc tatctttatg ttggagatga tgagaatgaa gaattattgg
     7801 aaaatgagtg atttttcgtt atttgcggtt ccttatgctt atgtagatca tagttcttat
     7861 ctggcggatc aattgtttgt gcagaataag ataactatga aatttaaggg agagatggtc
     7921 aaggatggtt cggcgtactg cattgtattc tgtaaggtgc taaagaggga cgtcgcaaga
     7981 tttgaggaag ctctagaacg acttaaagat aaaatgctct tactgggaca taaagattat
     8041 cctgccgcct gtggtgaaat tgcaaagatg attgaagaag acacgaaagc gaggatgaga
     8101 aaatgaagca atatgtaacg cttgataaaa gaagtaagaa agcgcagagg gaatattatg
     8161 ctaagcagcg aacaacatgg ggagaactga atccggtcac aagatcagtt ccgagtggaa
     8221 aggcttacaa cagaaaaaag gaaaagcaga gaatcggtgt tgaattcaga aatggatttg
     8281 atgccgattt tttgcgaagt ttagttttgc tcatacattt taggactagc gaaaggagcg
     8341 aataatcatg aagagatata agttttgcgg tttgtaatcg caaaacaaat tgccttttgg
     8401 aacgaattgc gatatactat ctacaaacgg aggtggtcac gatgatttac agacctttgt
     8461 atgtagagaa gatcatggct tatgtggata cgccatttgt aaaaatcctt acgggagtac
     8521 gtcgttgcgg aaagtcaacg atcctgaaaa tgattatgga gagattggaa acgcagcata
     8581 acattccaaa ggagcgcatt gtcagttgcc gctatgactc aatggaatat gaggatatga
     8641 cggcaaagca gatgtacgca cagcttaaag agcgtctttc acccgatgga aaaacctatc
     8701 tgtttctcga tgaggttcag gaaatcaagg gctgggaaaa ggtcgtcaat tcgctggcat
     8761 ctgactttga tgttgacctg tatgtgacag gttccaactc tcgcatgatg tcctctgaga
     8821 tctcaaccta tctgacggga cgatatgtat ctttccgcat cttcactttg tcctttggcg
     8881 agtacctgat gttcaaaagc caatataccg aggtgggaga gccgaaggcg gagcttgccg
     8941 actatgtacg cttgggcggt ttcccggcaa cgcatttgca ggagttttct caggacgagg
     9001 tttacactat cgtgcgtgat atttacaact ccacgatctt ctcggatatt gtcaagcgca
     9061 atcaggtgcg gaagatcgac cagttggaac gagtggtcaa gtacaccttc aacaatgtgg
     9121 gcaatacctt ctcggcaaag tccatttcgg attacctgaa agcggaacac cgtgcgttgg
     9181 acaatgaaac agtttatagt tacttggaga agctggagaa ggcgtatctg cttcatcgat
     9241 gctcccgctt tgatttacag ggcaaggaga tcctgaaaac acaggagaaa ttctatcttg
     9301 ccgataccgc cctgcggtac agcgttctcg gctataacgc tgacagtgtg gcatccagtt
     9361 tagagaatgt cgtgtacttg gagctttgcc gcagagggta taccgtcaat gtcggcaaat
     9421 ccggcagcag cgagatcgac tttgtcgcag tacggcaaaa tgaaaagctc tatgtgcagg
     9481 tgacgcagga gcttcattct ctgaaaacag aaaaacggga atacgaccgg ttgttggaaa
     9541 tccgcgacaa ctaccccaaa tatgttctca caacagatga gttctcggga ggaaactacg
     9601 agggcatcaa gacgatgcat atagcggact tcctgttaag tcatgaatac taaacgacag
     9661 aatcgcgaaa tggaagagta tcgtagtctt ttggagacta aaaagatgct atgcatagta
     9721 gaaaagaagg gaaaataacg gtatgggaat gactttatct atggaggaaa aggtacagac
     9781 agcagctgag acagtgatca atgcatcggc agctgacgaa acggtaacag gcgggaccac
     9841 accgtcagag ccgctttctg agaaaaggat agtgcatgca gaggattttg atccgctgtt
     9901 tgccatgtcc gaggaggatt gtgtcgaagg gtttataaaa cgtgaaatgc agcgtgccgg
     9961 gatcacggaa gaacagaatc tgatcccgga ggatatgctg cgcagaaaaa atatactgaa
    10021 tgccattttg gcggttctgt tattcgtata catatctttg tttttctttc attttccgat
    10081 gaaaacctat gtgatcggac tcctgatcct ggtggtatat gcatttttga ccagtcgcta
    10141 ccaactgata aaatatttga aaaaggagat ccgatccaga tcccaggaga aaatctcgaa
    10201 tattgtcatg aatgtgaagg catccatggt gccggattac agcaaaaagt taaaatgggc
    10261 gctcatagcg gtggctgttg caggctcgct gctgctgttc agtaagccca ggatcttcta
    10321 tgagcatgca gatgacggat attatgtacg cttttatgtc tatggtctga cgaatatgac
    10381 tacagcgacc atccccgata cctatcaggg ggagaaagta gtgggcctgc gcggcaacac
    10441 cttttccaat atgccctttt taacagaggt aacactgccg gacagcatta cagagatccg
    10501 cgggcaggca tttaaaaact gcagaagcct ggaacgtgtc acattgccgg agcatctgac
    10561 gtatctgggc ggggaagctt tttaccattg cagcagtctg gaggaggtta atcttccggc
    10621 gggactgacg gagatccgcg gcaacacctt tgaagaatgt agcagcctgt tgcgcataga
    10681 gatcccggat aacgtgacca ggatcggcgg acacgcgttt tatggaaata ccagtctgga
    10741 agaggtggtg atctccccgg acagcaagct gcagcagatc gggtcttccg ctttccggtg
    10801 ctgtgattct ctgcgggaga tcacactgcc ccggggcgta tctgtcaatg aacgtgcttt
    10861 caaggagaca cctgtaagga tcgattacta tgaatactaa atttattcat ttgttatatg
    10921 tgccgaccat ggcatgcaat atgcagtgcc ggtactgtta tctggaggac cacaccgtag
    10981 ataccctgcg gggcggagat tgcctggaga ccctgcaata tgccatcgcg aaattccgtg
    11041 aagcggatgt ggtgcccttt aatatttccc tgcacggcgg cgaggtgacc actttgccgg
    11101 agcaggaatt ccgcgacctg atccaatata tcagcagata ctatcaggac atgcgtgaac
    11161 tcatcacgga tgccggcttc agggtggggc atccccacat caagacgaat ctgtacggac
    11221 tggacagaca tatcgagacc atccgggagt tcaacgtgtc catatcgggc agcctggatc
    11281 tgcctctttc cctgcatgaa aaatacaggg tgaccaaggg cggagagggc acactggaac
    11341 ggatactgga taatatccga ctgctggagg agatccctga caagaaaaaa gtatctgcca
    11401 cgatctttcg ggagcatttc gaacagctgg atcagatcat cgaggatatc agattcctgg
    11461 accggaacac gtgcctggac atgaatgatt ttaatttcat gatagggttt gactacaatt
    11521 cctgtggcct gctgcatcac atgagtgaag aggaacagct gatcttttac cggcggatgc
    11581 acgaggcttt tgacgggacc aatctggatg ccggggtcaa cggtgcatgg ttcgatgagt
    11641 tcggaccgga atactgcacc aactgcgaca attgcggaga gaaatttttc ctattggagc
    11701 gtaacggaga catctattcc tgtgtcagag gtcagaagaa cgaagatttt tactacggta
    11761 atatctaccg tgacacggtg gacaccattt tgaaaactgc tgcacgcaag atcttccaaa
    11821 accataacag acaaccgttt cccgaggaat gtgcgaggtg cgcttacctg tatctgtgca
    11881 aaacgggctg cccttttgtg aaaaacgtat atggatccgg caaaagctac acctgcctgc
    11941 tgcaacagca gatgtaccgg gaccgcggct atgccccgga tgcttctgct gacgagacgg
    12001 catatgaata tgtgacgaag atgcgcctcg aggagccgga aaagtatctg cctgccaaga
    12061 catccgcaga atatcctgca ctggaacaga tcattgcaca agatgcgagg ctgaaatata
    12121 tctatgacag cggtgtgttt gaactggacg tggatggaga cagatatccg ctgatctccc
    12181 aaattcttag gaagtccagg gagatcctat atctgacacc catcagtacg gtaaagctgc
    12241 gtatgaaaaa gcatatgttg caggaagaat gcgattatcc ggagaacaac gcattgtacc
    12301 tgatgctgtt gtccggagat ctcgtgactt acggggatga agggagaaca aagcagcggc
    12361 acattgccac gcatcagatt tataaagggg tgctggacca ttccggggac aacgaagaag
    12421 aatggtatgt atatgatatt tccgggctcc tgcgcgaata cgcgaaggaa tatgccacgg
    12481 acagtccgaa caatctgttg tgtaccacta cggagctgag agactgccat taccggaagc
    12541 aggaaaataa tgcctattac catatacagg ccatcaatct cccctttcag aacattgaat
    12601 tttactatct gacactggag caaaagaatg acaaggaggc tttccatgaa ttttgaaccg
    12661 cagacctatg aagaactgat ccgcatgaag cgttgcgtgg aactgacaaa atattatgag
    12721 gtaacagagg aggagatttg ggagatctat catttcctgg agcaggagcc ggaggctttc
    12781 atcaagggag gcagacagaa tctgtccctc atcatcggac agaatactgc aacaacccag
    12841 aaggtgatca tggcgaactg caccgacagc agcattgacg gaatcctgct gtcacggacg
    12901 gaattcaaag tgttccctca ttacacacca tcctcaggct ccggcagcag cggtggttcc
    12961 tcttccaaca acaataataa caataacaac aataataaca acaataacag ataggacacg
    13021 aaagcaatgg cattcatatc cgaaacactt caaatgcgca ggcttacaga aagcggcgtc
    13081 aatatcaaga ctacttacat cgtcgatggt accctgaggt accggtccac agacggaacc
    13141 tgtcaggatg atgtgcacag tggtatccga tgccgcaaag agtatttccg gcagggaaaa
    13201 ttgctccata cagagaaaat cttcgattcc cgcatggagt atacctatgt atccggaatg
    13261 gatgaagagg cacagcatac ctgtgagaac tgcggcttta ccggcaaagt aaaggaattt
    13321 gtgaacggct gtccctgctg tgatgcggcc tccaacattg actacgcaga caaggagctg
    13381 ggcagcaagc accattatga cctggtattg caaagcccgg tctaccggat cgtcacggga
    13441 gtggtagact acatcatatc gttcctgcta tgcagcctgt ggatcataaa tacctccagg
    13501 accttcaatg gctatgatgt gggcaagatt ttcatctaca gcacaatcct ggctatgatc
    13561 ctctattact ttttctacct gtgcgatgct tatgcggtat tgggacctgt cagacgatac
    13621 aaagaaaaac agaaccgtcg gcagcaggaa ttctggaccg ccaccggcat cgacaaaaag
    13681 cggttctata ataacctgaa ctacgaagcc ggcagacgct actattcccg accggatgtc
    13741 attgattacg acattatgga ctataccggg ctgcaggagc atgtggagaa cggcatactg
    13801 tgcgtggatg tggagttgaa ggtcagactg gtgtatctgc gaggtggccg gatcacatcc
    13861 gcatatcaga aggatacctt ctccctcagc cacaacgaca gagtcatgac cctggacagc
    13921 ggaatccacg tgatcaaatg ccccaaatgc gacgccaata ttgatgtgac caaaggcgtg
    13981 tgtgaatatt gcgggaccga gatcgacagc ctgcaggaat ggaagtgaaa aattacaaaa
    14041 tgttgatggg aagtacattt tcgtattaca aaatgttgat ggagatatac cgaggaggaa
    14101 gaaacaatga aaatctggtt agatgatctc cgcccggcac cctggggata tgagagcgcc
    14161 agatccgtga atgaagccaa gacgctgatc cgcgaggcgg agagaaatgg cattgagatc
    14221 gaggtgctgg atctcgacca tgacctggga gattttgcaa atcagggcgg cgatgccatc
    14281 aaactgctgg actggctggt ggagcgggag accttttatc ccgtggagat ccataccgcg
    14341 aaccccgtgg gcagagcgaa tatggagagc gt
//
LOCUS       DAWDHD010000058        14025 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_290_length_14025_cov_7.939084, whole genome shotgun sequence.
ACCESSION   DAWDHD010000058 DAWDHD010000000
VERSION     DAWDHD010000058.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 14025)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 14025)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14025
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_290_length_14025_cov_7.939084"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3824 a         2886 c         4029 g         3286 t
ORIGIN      
        1 gggcgggccg tgggaaatgc cctggaagtg aaggaagcca ttgcaaccct gcggcgagag
       61 ggcccggaag atttcaaaga actgtgaatg gtactgggaa gtcggatgtt acttgccggt
      121 agtaaggcgg agagtgtcga agaggccaga aatatgctta caaatgtcat cagagatggc
      181 agtgctttac ggaagctgga gcagtttgtg gaggcccagg gcggtgaccc tgcggcggta
      241 acggatccgg agcttctgcc gaaggcatcc atggtggttc ctgtctgtgc ggacagagat
      301 ggctttgtag aaaaaattgc ctgtgaggat atcggaattt gttctctgct cttaggcggc
      361 ggaagagaga ccaaggaaag tgagatcgac ctgtctgttg gcatcctctt agagaagaaa
      421 aaaggggatg cggtgaaaaa gggagaggta ctgggatacc ttcatgccaa tgatgagaaa
      481 cgtctggaag cggcgaagga aaagttccgg agtgcctatt tcatcaccgg ccatcagccg
      541 gaggcagaac ctctgatcaa agggatttta cagtaacata ttccggcatc cgctttcata
      601 tagttttata tgaaaatgaa gagaatgcca tcgaaaaaag aaaaatggat cgattccctg
      661 cagttaccca aggatgtctg tatgggagca ctgcgggtga cgctgacggg aaactccgaa
      721 gcctggattg aaaattacag aggaatcctg gaatataccg gagagcggat ccttttgcag
      781 gccaagacct gtcaggtgtg cctggaggga aacagacttt ccatcgacta ttataccaat
      841 gaagatatga agatcagcgg aaatatcagt gcactgcgat atttgcgtga ataatcattt
      901 ggatgagagg ttaacaggat gacggagtgg gtcaaacgtc tgcagggtta tgtgaagatc
      961 cgggtatggg gctttgcacc gcagcgattt atcaatctgt gcagtaataa gggaatattg
     1021 ctgtggaata tagaaaaaca ggaagatgtt tataccatgt gcatcggcct gcggtctttt
     1081 tacgaactgc ggcccatcgc cagaaagaca aaagtaaggg tagtcatatc ggaaagatat
     1141 ggactaccct tttttgtgcc gggaatgctg cgcagaaaag cgtttctggc aggtttattt
     1201 ctggcggtgg ctttctggct gatttcgtct ttatttgtgt gggatattca ggtgactggc
     1261 aattatcagg tgacggatga cgtgttctat tccttcctgg agcaggaggg cattcacacc
     1321 ggaatgcgaa ggagtgaact gaatataggt gagctggaaa aacagataag acggaagttt
     1381 tcgcaggtta cctggacctc cggccgtctg gacgggacca gactggtgat cgaagtaaag
     1441 gaaaacgata tgtcggttcc agaagtcgtg caggatgcgg caggggcagg aaaagatctg
     1501 gtggcagaat gcaacggggt gatcacggat atgatcgtgc gttgtggtgt cccaaaagta
     1561 tccataggca gtgaagtggc aaaaggagat attctggtgg aaggcaggat ccccatttat
     1621 gccgaggatg gaaccgtcag ggaatatcgg ccggtgaccc cggatgcgga tatagtgctg
     1681 gagcataccg gaatctttca ggcatatctt ccggcagatt atatccggaa aaaatacacc
     1741 ggaagacaaa agacatttta ttttatccat ttcggtgacc gggagtggaa accggagaaa
     1801 aaaatggatt ttttgcaata tgacagcgtg ctggaagcat gtcccgtaaa agcgttggag
     1861 tatctgcaga tcccctgcat ggtaggcagg atcacgtaca gagagtatca gaaaactgaa
     1921 tattattata cagtgtcgga agcagaaacg gttttacaga aaaaaatcat ggattttctt
     1981 gaaactttag atgaaaaagg ggtacaaata atctcgaaag atgttaaaat agaaacaaag
     2041 cgcgccggct ggaccgcaca cggggaactt ttgctgcggg aagcggcggt cagtctggta
     2101 gattcccggg cggaggatac cggagcagaa gatttggaca gaaatgagga ttaagacata
     2161 aatgagtgtg acagcaatcc gtctggaaat ggatacagaa cagatgcaga agatcttcgg
     2221 tatgcaggat gcgtacatca agaagctgga acaggattat caggtaacta ttgtggaccg
     2281 caacggaagt gtaatgatca ccggggaaga ggaaaacgtt tcgaaggcct cccgggtatt
     2341 acgacagctg accgcattgt cggatcacgg caatgagatc gaagaacaga gcgtggatta
     2401 tgccattgaa atgggaaaag aagaccagga ggaaaaactg atggagatgg atgcggactg
     2461 catctgccat accatcagcg gaaagccgat caagcccaag accctggggc agaaggctta
     2521 tgtggatgcc atccggaaaa acatgatcgt attcggactg ggaccggccg gtaccggtaa
     2581 gacgtatctg gccatggcca tggcgatcac cgcttttaag aacaacgagg tgagccgtat
     2641 catcctgacc agaccggcca ttgaagccgg agaaaagctg ggctttttgc ccggtgatct
     2701 gcagagtaag gtagacccgt atctgcgtcc cctgtacgat gccctgtatc agatcatggg
     2761 agcggaaagt tttgccaaaa atatggaaaa gggtctgatc gaagtggcac cgcttgccta
     2821 tatgagaggc cggacactgg acaatgccta tattattctg gatgaagcac agaataccac
     2881 accggcacag atgaaaatgt tcctgacccg tatcggtttc ggctccaaag tgatcgtaac
     2941 gggtgactcc tcccagaagg atcttcccgt gggagcaaaa tccggtctgg atgtagcagc
     3001 cagagttctg aaaaatatcg atgatattgc cttctgtacc ttgaccagca aagacgtggt
     3061 aagacatccg ctggtacaga agatcgtaaa agcttacgat gactatgaag cccggacgat
     3121 caaaagcaaa agaagtccga gattaaacaa acgtccggat aaaggaaaca gataaatatg
     3181 aagaacaaag agaaaaacgg attttcgcgg cttttgctgc cggagatcct tacggtactg
     3241 atcggaggtg ccgcagtcta cggactggga cttttgggaa aacagttgtc tgtggaaaat
     3301 gccctgcggg atgccgtgat ggcagcactg ggacttgcag tgtcaggatt tttcctgcgc
     3361 agggaagtag tggattcccg cctggattat gataacggag agcacctgat gagattctgg
     3421 accgctgtct ggtgcagcct tttgttttcg ctgacctgtg cattccttcc ggcgggagga
     3481 tggccgtttc tggcggtctt tgtggtattg tccctgttca gcaatctatc agtgggaatt
     3541 gtgttttccg gggtgtttct gatgattgcc acgctgtggg gacagtcggt ggccactatt
     3601 cccctgtata ttctcagctg tgcgcttgca gcctgtccgt tccagcaatc agagcaggaa
     3661 tttgccatcg ggatccccct gttcttatct ctgttctgcc ttttgctgtg cgagaccgca
     3721 aatgtggtac tgcttgccaa tgaacattta agtctggaac agtttctggt tccggcagca
     3781 aatctgatcg tgtcgggaat actgcttctg ggtattttga aaattttttc gggtacagtg
     3841 gtgttcaggg atcgtgtaaa atatctggaa ctgaatgaca ccgagaatca ggtactggta
     3901 aagtaccgcg aggaagaccg atccgaatat ttcctgtgtg tacatacagc ctatttctgt
     3961 gagcggatcg caaataagct ggaactggac agggatgccc tgaaatgtgc cggattatat
     4021 cacagaaaag gctgggatct tatgcaggag acaccggata tggaattccc ggcaggcgca
     4081 tcggagatcc tggaagaata taaaggaacc cggaaataca aaaaagcgga gaccgcagtg
     4141 ctgtattgtt cggatgcggt agtcagtgcg atccttttgc tgttgcagaa ggaaccggag
     4201 aagaagccgg attatgagca ggtgattgac aggatttttg agagaatccg ggaaaaaggt
     4261 attttcagcg aatgtgacct gtccttaagg ggctggaacc ggatgcagaa gatattcaag
     4321 gaggagaaat tatactatga ctttttacgt tgaaaatgaa accgatgaga cattttcaaa
     4381 tggggaaaac agtttcccca tagaggagac cgtggagcag gtagccacgg cggttctgga
     4441 tatggaggga tgtccctatg agatccagtt aaatgtactg ttgacagaca atgacggtat
     4501 tcatgcttac aataaggaat accggaagat tgaccgggag actgatgtat tgtccttccc
     4561 caatctggat ttcgatgagc ctggggattt tgaaatctct gaggacagag aggcagatta
     4621 ttttgatccg gatacaggag aactgatcct cggggatatc attatttctg tggaccgggt
     4681 caaagagcag gcggaaagct atggacacag ccagaagaga gagttcgcat ttctggtcgc
     4741 tcacagtatg cttcatctgt gtggctatga ccatatggaa gaagatgagg caaaggtaat
     4801 ggaggcaaag caggagcagg ttctcgcatc ccttggaatt acaagagatt aaaaagggcg
     4861 tgtcatacac gtaaaggtag cttacatgaa tcaggtggaa gtaaaaagaa gacaaataca
     4921 gagtttttcc tatgtccttg cctgtatcaa tatctgggtg ttcgggaaaa gcatcggaga
     4981 taacggcctg ggatatctgg cagcggctgt tctggtattt gccctgttct gggtgcttac
     5041 cgggaaaaat ctgccggaca gaatgggcag aatgcttcgg ggcagaaatg ccagggggca
     5101 gtatcggaat gtctccagaa tgcgtaagaa catgatgctg ttccagatcg cagagggact
     5161 tcttgggact gttttgtgca tgagtctggg ctggattctt ttggaaaaag tctttctggt
     5221 gccttacgga agcatgatcc tgtggatctt aagcccggca ctttttcttc gttgtatcca
     5281 gtcggtattt ttaggatatt ttcagagtga aggttcggaa atgccatctg cggtatcttc
     5341 ggtgctgcgg caggtatttt tcctgggcct gggtctggtg ttcctgggga ttttccggaa
     5401 ctacggggag aaagtaagtc ttttattaaa aagggcagac tttacatcca tgtacggagc
     5461 catgggtatc gcagtgggaa tgctgctttc ggaggtgttg gtactgctat ttgtctttgt
     5521 gatctaccgt ggcagcatgg caggcagaag agaagcggaa agcggtatga agggaacgga
     5581 ctctttctcc ttgcagctgc gtacattact gattcctatg ggtggggata ttctcagaga
     5641 catgttttta ttactgcctt tgtggatcgg cctgcttttc ttccagaaaa gatcggcgga
     5701 tatttatgca tccgtttctg cgtatggtat tttcgtcggc agatatctga tcacgatcct
     5761 gatgcccgca gcaattttaa gtgcgggaat tctgccgggg gtggccagaa ccggcagcca
     5821 tatccgcaaa aaagaagaaa gatatgccag aactgcattt caggcaggag tacaggggac
     5881 ggtggtacat ggattctttt tcacggcagg gatcgcggta ctggcaacac cactgtcaca
     5941 ggctttggat aaatctgcgg aaatggcact taccggtttg ttcactacgg gatcttcgct
     6001 gattctgtgg cttctgcttt tgttttattt ttcggagata ctggaacttc ggggggaaaa
     6061 gcatctgctc ctggcaggat acggcattct ggatatcgtg agcatcgtga tattgctgat
     6121 tcttctgaat acgggaaata caacaacggc attatcttta agccttgtgc tggggacggc
     6181 agcgggagga attgttctcg gcatacttgc ctgccttcga atgaagacct ggccggatct
     6241 gttgcggaca ctggcaattc ccgccggaag ctgctgtgcc tgcggattgc tggcttttgg
     6301 gctggagaag atctgcctgc cccatctggg ggcgatggta acggtgctgc tggaactggt
     6361 gatcaccggt gcggtctact ggtttttgct tttgatgtta cgcagtttcc gtggacagga
     6421 tctgaattat gttcccggcg gtaaagtgct gcgtgctttc ggaaaaacgc tccggctgct
     6481 ctaaaactgt tttacggatg tatattttcg ataaaataac tgatactgat acaaagagtc
     6541 aatagaaaca tcatggatgt gaggcagtat gaaatttgta aaacgtatca gttacttttt
     6601 tgtgattccg gttgtgtttt taattcttgg aatctttctt ggtgtctggg gcattcattt
     6661 tttctatccc ggacaatggc aggatctgaa tgagaaccga cttgccctgc cgctcacgga
     6721 agcggagact gcggcagaca cagatcctta tgaggtggca cccagtgtgg aggcggcagc
     6781 ctccagggag acactttgtg cggataccga atatgtgttg gaagaggcag atattgtacg
     6841 gggtaccaca gtggagacct catggaagat cccacataaa tatattggga tgaacaggga
     6901 aaattttctg gagaccatgg aactctatgc ggcccatccg ccattgtcgg agatcgagcg
     6961 gggctttgtg gggttggagg tattgtcttt ttcccgggaa aaagtaatgg tccgtatgga
     7021 ttaccgttat gtccagccca gcgcaagttt ttatctggcg gtgtcggaca atgaagtggt
     7081 ggtctatctg gaagacagat ccacggtata tatcaatacc gggatctcgc tggaagaatt
     7141 gccggaacag atacagctcc agatcatgga catgctgttt gttccggatg aagaaacatt
     7201 gtatgatttt ctggagacgt attcgtccta gaattctgaa taaaggtgat gcagatgaaa
     7261 aaacagatcg gtgtaatcgg cggaggcgct gccggaatga tggcagccat aaccgcggca
     7321 aaagaaggcg cacaggtgac gatcctggag cgtaatgacc gtgtgggaaa gaaaatattg
     7381 cagacaggaa acgggaaatg caatctggga aatcgtgcgc tttccgtgga gtgttatcac
     7441 ggcggagatt tggaatggat ccgggatgtg ctggaacatt ttgatacgga ggatacgata
     7501 cgctttttcc aaagtatcgg tctgatggta aaggagaaaa acggctatct atatcccgtg
     7561 tgtgagcagg cggctgcggt gctggacgtg ctgcgttatg aattacaggc actcggtgtg
     7621 gagatacagt atcagtgcaa gatcacaaag gtcgaaaaac tggcctccgg aaagctgcag
     7681 gtcagtgacg gagaaaagac atggcagttc gatgcggcga ttgtgacctg tggtggaaaa
     7741 gccgcaccgg ctaccggatc ggacggcagt ggatttaaaa ttgcaaaaaa aatgggacac
     7801 acctgcattc cttcagtgcc cgcactggtg cagctgcgct gtcaggagac cgacctgcta
     7861 aaggggattg ccggtgtccg ggcggacagt gagatccggg tcttctgtga cggcggggaa
     7921 atatgccggg aacggggaga actacagctg acggattacg gcatctcagg cattcccatt
     7981 ttccagctaa gccgcagggt gaactatctg ctcagggaca ggcggaacgt gacagcacag
     8041 attgactttt tgccggatct gacacaggaa agctatgaag aattacaggc agggcggaaa
     8101 cttttacagg gagaccggac agcagaggaa ttctttaccg gaatgttaca taaaaaactg
     8161 accgctgcgc tgctaaaaat ggcagggatt cgtctggcta ctcccatgaa ggagatcccg
     8221 gaggaaaaga ttcatgctta ttataatctc tgccgtaacc tgaaactgca tatcacagag
     8281 agcaatcctt acgaaaatgc acaggtatcc gccggaggcg tggatgtacg ggaaataaag
     8341 cctactatgg aatcaaagat ctgccccggg gtctattttg cgggggagat cctggacgtg
     8401 gatggccgat gcgggggcta taatctccaa tgggcctgga gcagtggtta tctggcggga
     8461 taccacgctg cgttagcata aaataatttt ttacacgtga cgtatgcctg cgtaaggtgc
     8521 aaaagctccg gaaaggcggc aggtcattga aagatatgag gatgaaaatg atcagattaa
     8581 atcagctgaa gcttccggta gatcataccc gggagcagct tatacataag acggcacagt
     8641 acttacggat cccggcggcg gatatcctcg aactgcagat cgtaaggcag tctctggatg
     8701 ccagaaaaaa gccggcactt ttttacagtt acagtgtgaa tgtaacggtg aaaaaggaag
     8761 aaaaagtata taaggatgcc tgccgcagac ttggaaaagc caatgttctg ctgaccgaaa
     8821 aaacggaata tctttttcct gcagaaggtt caacgcagca gaagcatccc actgtgatca
     8881 tcggaatggg tcctgctgga cttttttgcg gatattatct ggcacagcac ggctatgcac
     8941 ctgtcattct ggaacggggt gcagacgtgg atgccagaca ggcggatgta gaagctttct
     9001 ggcagacggg cagattaaag ccggattcca atgtgcagtt tggagaaggc ggtgccggaa
     9061 ctttttcgga tggtaaactg aatactttgg taaaggacaa atacggcaga aatacggaag
     9121 tattgaaaac tttcgtaaag cacggtgctg atccggcaat tctttatcag gccaagcccc
     9181 atattggtac ggatgtcctg agcaaagtgg taaaaagcat gcgggaagaa atattgcgta
     9241 tgggtgggga agtccgattt catagtcaga tgacggagat ccttacagag aacgggcagg
     9301 tcacaggagt aaaggtgaat gacacggaag tgctcccctg tgagcaggtg gtactggcac
     9361 cggggcacag tgcccgagat accttttcca tgttgtaccg gaagcagttt cccatgtcgg
     9421 cgaaaccttt tgcagtggga ttccgtgtgg agcatcccca gagcctgatc aatatgagtc
     9481 agtatggcgc tgagtcggta aaaggtctgg gtgcagcggc ttataaggtg acggctaaga
     9541 caagcaccgg cagaggagtg tattctttct gtatgtgtcc gggcggctat gtggtcaacg
     9601 catcttccga gccgggaaga ctggcggtga acggtatgag ctacagcggc cgtgacggga
     9661 aaaatgccaa cagcgccatt atcgttgcgg tgactcctgc ggattacggt tccgatcatc
     9721 cactggcagg aatcgaattc cagcggggac tggaacagag agcttatgaa ttatgcgatg
     9781 ggaaactgcc ggtacagcgg tacggagatt ttagggagaa agtcaccgga gagcatcctg
     9841 cggaaacgga cggagtacaa agaagtggcc cggaagcatt ggaaccacag tgtaaaggtg
     9901 cttatgaatg ggcggatctt accggaattc ttccggcaga gtgtaaccgg gccttcgtgg
     9961 aggggatgga cagctttgac agacagatcc acggctttgc ctctccggga acgatcctga
    10021 ccggtgtgga aagcaggacg tcttccccgg tgcggatcga acgggatgaa gagttacagt
    10081 ctgccatccg gggttgctat ccctgtggag agggggccgg atatgccgga ggaatcactt
    10141 ccgccgccat ggacggtatc cgcgtagcag aacagatcgc ttcacagttt gctccgctac
    10201 agcgttgacg tatggcagaa aatgttatat gattcaaata ttcagagatt gcatcctgca
    10261 gatcgatttt tcgggggagg cgctgaatat attaacctaa taatatgatg tcggggtcaa
    10321 aagcccccga ctttgatgcc tacggattgc tccgtgctac gcactcccac aatccaccca
    10381 atattcgcat atcgtgggat tatcatccca cttttatgct catatcgggg cgggtcaaaa
    10441 ggtcttttac ccacctatga gcaagctcat gtgggcttag accttttgac cctggcatca
    10501 taatatggac ttagaaaggc aatcacgtga ataacataag ggaacaaatc aaggataaga
    10561 aaagaatcgt cgttaagatc ggttcttcct ccttacagca tgccgagacg ggagacctgg
    10621 attatactcg tctggaaaag ctggtaagag agctttgcga tatcagcaat caggggaaag
    10681 aagtcgtact tgtgacttcc ggtgctatcg ccgccggaaa acaggcagtg catttaaaga
    10741 ctacggaggg gcagactcag gcagaatcca tggcgatcaa gcaggcctgt tccgccatcg
    10801 gccaggcaag actgatgatg acctaccaga agctttttgc ggagtacaat caggtggcag
    10861 cacagatcct gatgacgaaa aataccatcg tggacaatct gaaccgctac aatgcccata
    10921 ataccttcac ccagctattg aaaatgggag caattcccgt tgtcaatgaa aatgatacgg
    10981 tagcgaccta cgagattgag atcggtgaca acgataccct gtcggctatt gtggcagcac
    11041 tgatcgatgc agatctgctg atcctgttgt cagatatcga tggcctctac accgatgatc
    11101 ccaggcagaa tcccaatgcg gaatttatcg aacaggtgga tgaactcacg gaggaattca
    11161 tgcatatggg caaggccagt accggcagca atgtggggac cggcggcatg aataccaaaa
    11221 tgatcgcggc gaagattgcc accagttcgg atgtggatat gatcattgcc aacagcaagg
    11281 acatcggagt acttcatcgt ctgttatccg gtgaaaacga aggtacttta ttcgtggcac
    11341 ataaggatga aagctttgat ctgccggaat ttgtacagaa tctgcacaaa aatcagtgat
    11401 gatacgtcaa cgaaaagagg aatacattat gaatatggat gaaagaataa agcgcatcaa
    11461 tgagctttat cataaatccc agagcgaggg actgaccgac gaagaaaagc tggaacagtc
    11521 cattctcaga caggaatacg tggacagcat caagcgcaat atgaaggcgc agctggacag
    11581 catcgaaatc gaaaggccgg acggtaccat tgaaaaatta aagccgtcct ataaaaagaa
    11641 gaaatgagca ctgaagaacg cagagaagtg aaaaagaaac tgcgcaggga agtgctggcc
    11701 cgcagggcgg cactgacgga gccggaacgg gagagaggga atcttctcat taccgagcgg
    11761 attctgggac accagtggta ttacctctcg gatacgattc tgggctttgt cagctacgga
    11821 agtgaaatct gcacgacaga gattctggag aatgctctgc aggatggcaa acgtgtctat
    11881 gttccgaagg tggaaggtca ggaaatgcgt ttttaccgga ttcaatccct ggcggaattg
    11941 aaagagggct ataagggaat cctggagccg gaaggcaata cggagagcta tgaatatgct
    12001 cccgaagagg cagcaaactg tctgcttctg atgccgggaa gtgtatttga tcccctgggc
    12061 aaccggatgg gatacggcgg cggattttat gaccgctatc tggcagacag ggaagcctta
    12121 agacttcgca gtattgctgt cgggttccgc tgccagcagg tcgaaagtgt tcctgtggaa
    12181 gagacagatg taaagcccta tcaggtgatt ttggtataaa tctttggtgt tggaagggcg
    12241 gggaaggaat gtcggaactt ttagaactgg gaaaaaatgc ctgcgatgcc aaatatgtat
    12301 tgcagaagct tactacagaa gagaaaaaca aggcgctgca gacagcggca aatgatctgt
    12361 gtgaaagaca ggaagagatc cttgcagcca acgtactgga tctggaaaac gggaaaaaca
    12421 acggaatgaa tccgggactg ctggatcgtc tgaagcttac cggagagaga atcgcgcaga
    12481 ttgcagaggg ccttcggcag attgcggctc ttccggattg catcggtgag accatagaac
    12541 atttcgaacg tcccaacgga ctgaaaatcg ataaagtcag agtaccgtta ggggttatcg
    12601 gcattatcta tgaggctcgt cccaacgtga cggcggatgc cttcggattg tgctttaaga
    12661 cgggaaatgc agtgatctta aagggaggca gcgatgccat tcattccaat atcgctattg
    12721 tcaaagtatt acaggatgca ctggaagcct gcagtatgcc caggactgcc gtacagctga
    12781 tcgaagatac ggatcgtgcg gtcactgcgc agtttatgaa gatgaaggaa tatgtggatg
    12841 tgctgatccc aagaggcggt gcgggactga tccgcagcgt ggtggagaac agcacgatcc
    12901 ccgtcataga gaccggaacc ggcaactgcc acgtctatgt ggacaaggat gcggatgtgg
    12961 atatggcggt aaaaatactg atgaatgcca agacccagcg gatcggtgtc tgcaatgcct
    13021 gtgagtctct ggtggtgcat cgtgccgtcc gggataagtt ccttccgaag atggcggaag
    13081 ccctgaagac gaagaacgta gaactgcggg gagacgaaag cacccgggag atcctgccgg
    13141 actgcattcc cgctacagaa gaagattacg gtacggagta tctggattat attctatctg
    13201 tcaagactgt ggacagcgtg gaagaggcca ttgcccatat caaccgttac aatacgaagc
    13261 attccgaatg tatcgtgacg gaaaatacgg agactgcaga gaaattttta aacgaagtgg
    13321 atgcagcctg tgtttatgtc aatgcctcca caagatttac agacggtttc gagtttggat
    13381 ttggcgcaga aatcggtatc agcacccaga agctgcatgc cagaggtccc atgggcctga
    13441 aggagctgac ctcctataaa tacaagatac atggaaacgg tcagatccgc agataagcat
    13501 ttgcgacccg ccacaggcgg ataatctgcg tattctccgg ctgggtattt tgtcctgtat
    13561 gcaaagacat ctgtcggaaa taactataga ggaagaggag aaaatttatg aaagaagtaa
    13621 atggaagtat ttggcacact atgagcacca agagagtcac tcccacagat tttgtctgtg
    13681 tcatcgagat ctccaagggc agcaagaaca agtatgaact ggacaaggag acaggccttc
    13741 tgatcctgga tcgtatcctg catacctcca cccattatcc tgcgaactac ggttttattc
    13801 ccagaaccta cggtgatgat ggtgacccgt tagacgtatt gttactgtgt tccgagccgg
    13861 tacagcccat gacactggta agagcgtatc ccatcggcgt gatctccatg ctggacaacg
    13921 gcaaaaatga cgagaagatc attgcagttc ctttcaatga tcccaattac aactgctatc
    13981 atgatatttc cgagctgccc agtcatgtgg tggacgagat ggagc
//
LOCUS       DAWDHD010000059        13928 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_292_length_13928_cov_12.039213, whole genome shotgun sequence.
ACCESSION   DAWDHD010000059 DAWDHD010000000
VERSION     DAWDHD010000059.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 13928)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13928)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13928
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_292_length_13928_cov_12.039213"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3613 a         2932 c         3841 g         3542 t
ORIGIN      
        1 aactctcaat ttacaaaaca tttaaaaaca gtattgactt tttccaggca tatggtaata
       61 taaacatatg aacagtggtt catatgaatg aatattaaaa ctaaatgcaa atcattagca
      121 actaaacaaa aaatgcgaat ggagaaagag gaggatatta ccatgaagaa aacttacaag
      181 atcgaagtag actgtgcagc ttgtgctctg aagatggagg atgcagcgaa gaaggtagag
      241 ggtgttaccg atgcaaccgt cagcttcatg acccagaaga tgaaggtgga atttgccgat
      301 ggcagcgatg agaaggccgt tatggagaac gtggtaaaag cctgcaagag agtagaggat
      361 gactgcgaga tctatttata ggatgctctc ggaaaagagg aagctatgac aaagaaacag
      421 aaaaaagtac tggtccgcat cattgtggcg gcagtactgt taattatttt gcattttgta
      481 cctctggaag ggatctggct gggactgctg tacctgattc cctatgcggt gatcggctat
      541 gatattctca gaaaagccgt tctgggcatt atgcatggtg aagtgtttga tgagaatttc
      601 ctgatggcag tagctaccgt aggtgccatg ggactgggag agtattccga aggcgtggcc
      661 gttatgctgt tctaccagat cggtgaactg ttccaggcgg tagctgtggg caagagccgt
      721 cagaacatca gcgccctgat ggatatccgc ccggattatg ccaacattga ggcagaggac
      781 ggcagcttag agcaggtaga tcccgatgat gtagagatcg gaaccgtgat tgtggtacgt
      841 cccggtgaaa aggttcctat cgatggtgtg gtagtggaag gtacatccac actgaatacc
      901 agtgccctga ccggtgaaag cgtccccaga gagatcacag tggatcagga tgtgatcagc
      961 ggctgtgtga acctgaccgg attacttaag atccgtacca ccaaggaatt tggagaatcc
     1021 accgtatcca agattctgga tctggtagag aattccagta tgaagaagtc caagtctgag
     1081 aactttatta cgaagttcgc aagatattat accccggcag tctgctacag tgctctggca
     1141 ctggccatcc tgcctcctgt gatcaacctg atcatgggca atccggcagc ctggagcacg
     1201 tggatcattc gtgccctgac tttcctggta atcagctgcc cttgtgcact ggtcatttcc
     1261 attccccttt ctttcttcgg aggaatcgga gcagcaagtg cctgtggtat tctggtgaag
     1321 gggtccaatt atctggaagc gctgtctgag accaaatatg tggtatttga caagaccggt
     1381 accctgacca agggtgtatt cgatgtgacg ggtatttacc ccggcaggga tttcacagag
     1441 gatgaactgc ttgagtatgc tgcatatgcg gagagctatt ccagccatcc catcagcaag
     1501 agcctgaaga tttcttacgg caaggagctg gaggcgtcca acgtcagtga tgtggaagaa
     1561 atcagcggac acggcgttac tgccaaagta aatggcaagc aggtagcggc cggtaatgcg
     1621 aaactgatga agagcctgaa tctgtcctat acggaaaata ccggtgtcgg tacagtggta
     1681 catgtagcgg tagacggaaa atatgccgga tacatcctga tctctgatgt gatcaaggat
     1741 ggtgcgaagg aagccattgc atctctgaag aattccggtg tgaagaaatg cgttatgctg
     1801 accggtgaca gcaaaacagt tgcagaacat gtggccggtg agttaaaatt ggatgaggta
     1861 cacagtgaac tgcttcccgc agacaaggta agctgcgtgg agaagctgtt gcaggacaag
     1921 agcgagaagg aaaaactggc attcgtgggt gacggtatca acgatgcacc ggtactttcc
     1981 cgtgcggata tcggtatcgc catgggagcc cttggctcag atgcggccat tgaggcagcg
     2041 gatgtggttc tgatggatga caatcctgcc aagatcgctc tggctatgcg gatttcccgg
     2101 aaatgtctgc gcatcgttta cgagaacatc gtatttgcgc tggcagttaa ggccatctgc
     2161 ctgatcctgg gtgcgctggg tatcgccaac atgtgggttg ctatcttcgc agacgtagga
     2221 gttatggtac tggctgtgct caatgccatc agatgcctgc gcattaaaaa caattaaccg
     2281 gatgcaggca aaatatgcct gcgtcatgag atcaccggta ttccgggaga acaggtggac
     2341 atgcagactt ttttttcaga aattacagca ctgtgcagga acgaacaatt tctgcacagt
     2401 gtgatggaaa aatatcatta tgatccggaa gaatacccca atctgaagag gctggcaggt
     2461 gagatgatcc cctgcctgga gacagaagcg ggatggcagg acagagaaat ccaagagact
     2521 tttcccggga gggatcagtc ttttttggct gtaggtatta ctctgggaaa agggatcgat
     2581 gaactgcagg aaaagtattt gcagaacgga tttctgacag agagttatat gatcgaagta
     2641 ctgtccagtg aattattatt agaaagctac cgggcttata cagagtgggt ggttgtgcat
     2701 cggaatctgc atgttgccag actgcatttt ttaggtgggg agatttctga gacttcagaa
     2761 caaaaaataa gcagtctgct gcggctggcg aaccttccca tgcttctgca ggagcttgca
     2821 ttgccggtta cttgtaatgc agcgtattgt atgatcccga aaaaaagtgt ggtattttat
     2881 gcggagttga cgaaggatcc ttttaccaaa tgtgccggaa tctgtctggg gtgcggcagg
     2941 agagactgcc cgaaccgcat ggaggaaaag gagaatttcc ctctgcggtt tgcggacatg
     3001 acggaccgtc cgctgtccta tgggtatgcc agaatttttt ccaaagcgga tgtaaaatag
     3061 gcttatagcg tacaaatcgg cagaaagtat tgtccgaaat attaaaattc tgataagatt
     3121 tatcagacga cttgtaagta tagaaagaat atggtatgat gcaatcactt tcaaagagga
     3181 gagatatcgc gtgtacggtg tctttcctct ttttatgtat acaatgagtc aaacacggat
     3241 ttgtgcctgc acaggaggct gtatttgacg aagatacatc atcaagcgtt ttttattcgg
     3301 tgatcaagta aaagatttga gaagagaggt ggcttatgga ggtattttta gaggtaacgg
     3361 tacagcataa aaaatatcat gcgggattta tggaaagaat gcgacagcaa ccggaagtca
     3421 catcctgcta tcaggtagag ggggaatatg atttcctcgt aaaagcggat gtgacctcgg
     3481 aaaaggaact ggaaaaactg gagcgcggcg tgcgtaagat taacggtgtc tgcagtgtgc
     3541 agccggtcta tgccctgcag gagatcatat agtcataccc ggagcagggc attgaaggtc
     3601 tcctctaaat tcagcctgcc gtagccccat tcccggttgg ggtagttcag accctgtgta
     3661 cgcacggcac cgcgtatgaa ataactgcgg atctcccggc tttccgccag ccggttattg
     3721 ccttcaatga ctgaccattg gaaaaactga gccacagctc cggcagtgat agcggcagcc
     3781 atgctgcttc ccgtacggcg tccgtcaatg gtggagatat tgactccggg agcggataag
     3841 tccggacgga tctgcccggt gcgcccaaag cctctgccgg attctgcgta aaaactgttg
     3901 ttttccgcgt tgtaagtaga aacgctcaga acctccggag ccatcgaggg ctccgtcaga
     3961 gtcatgtcgg gatccggatt gagaaaatat accggagtag acagaaattc tgtgataggc
     4021 agccacatgt gaaaattgcc gttatataaa ctaccggaag cagagacatg aaaggtccag
     4081 ataccggggg taggatcctg gatgcgtaca aggatcagtt cttcaccgga attttcttcc
     4141 acaagagcag agtcgatcgt gactctgctt ttttcgtaga taaaggaata ggtcacgctt
     4201 tgctgcaacc caaggcgcag ttcgggaata ttttccccac cgggagtgcg gatgctcaga
     4261 ttaaaagcat ccggcagagc gccccaaagc tccagccaga atccgataca gccttcactg
     4321 acccggatct ccacatcccg ggaagattcc tccggtgtct gagcgcttaa tcttccacgg
     4381 taatggtgag aggcgttccc ttcatttcca ccgcagacaa tggcggcacg gcttctgcgg
     4441 acagcgagag aagagagata ccgtgacagg gaggaattgc ccgcatgatc tccctggttg
     4501 gttcccagac ccagacagat gactaccgga cgctcgaaga gctgcacgaa ggattccgcg
     4561 tatttgatgc ccagcataat atcattttcc tgataggcgg gaacaccctc gggcaccaga
     4621 taaaagcttc gtagatattg cttacattcc ttgagtttta ccacaacgat gtcggcaccc
     4681 ggtgcagcac cgatataagg attgccttgt cggacgacac tgccggcggc gactccggcg
     4741 aggatactgc cgtgtccgtt ttcgtcccgg gagggaacga tggaataagg atcctcggag
     4801 cgcagcgcca ggttaatatc ctctctgcgg tattcagaac catatttaaa cccctcaggt
     4861 ggtatcccgg tctgaaccgt ctgatcccag atcgccagga tgcggctgtt gccgttctcg
     4921 tccagaaaca caggattttg ataatcaata ccggtatcaa taaaacacag gatcacgccc
     4981 cgccccgtga gacttagggg cggacgctgt acctgagtga tcccactggc aatcagactt
     5041 gccggatcaa agggagcggc accgggtggc gtctcaccgg agggagatag ctgcatgagc
     5101 ccgtaaagct tcggcatatt gtgatagggg aaaaaatcaa tcttcggatt gcggattccc
     5161 agattgccca gatacaaaat caaaaaatct tctccgatgc gttcataaca attattgtca
     5221 tctccaagaa attccgatgc ggaaaagtct gtgataatgt cataagtgtc ctctgacagg
     5281 atcatttcct tgcagtccat ggcgttactc catatcatac gaaggagtta gattaaccta
     5341 tgcggaaggt tcgtttttgt tgcatattga ttgatataat gataaaataa atagaatatt
     5401 ataacgaaag agacaggaac gttacatatg gaatttattc ataaaatcag agcattgcag
     5461 ataggaaaaa gaaagacttt ccgaagagtt ttgctggcgg aaatcctgct gatattattg
     5521 ggaattgtcg gtctgtttgg cagaaatgag gtgtatgaat atccgtccga aggggtaacg
     5581 gtaaataccg gagtctatca ggacaatatg actatagagc atatttcatt aaagccgggt
     5641 ctctaccgga tccagctgca ttatgatacg gataccaata tgaagaattt ctgtattgtt
     5701 gaggatgata ctgtgggata ccggatgctc cgcaacagcg gagagcacct ctatcaggga
     5761 ctgcatgaga cggattatcc gttatggctg ctgcagaagt cggataatat acgtgttact
     5821 gttacctacg gtggagaagg aaactatatg atctcggggc ttaccattcg ggaaaccaac
     5881 ggtctggcag tcattttcct gtttttggtt gtggtgatct ccattctggt gaatctgtta
     5941 tatacttatg tgcagtatga cagagagtat tcgttatctg tttcgcagaa gggaattcat
     6001 ttcggtctgg cgctcctttt gctgttcagt tccctgcctc tgctgcagga taccatgctg
     6061 tccagcggag atctgggata ccatatgctg cgtatgatgg gaatcaagga tggcattctg
     6121 gcagggcagt ttccggtacg gattgcaccg aaatggcagc agggctacgg atatgcgtct
     6181 tcgatctttt atggggagac acttttgtat gtgggtgcat tgttccggct gatcggcttt
     6241 tcctttatta ccagttatcg actgtttctt ttgtttatca atgcacttac catactggtg
     6301 gcatattaca gctttcgaaa aatttttgaa gaccgtaaca ttggcctttt atgtacagta
     6361 ctttatactt tgtcggtata tcgtctctcc aagacatata tctgcggatc gctgggtgag
     6421 actttcggaa tattatttct gccactgatc gcctgcggat tttatcttgt atttgcacag
     6481 gatgttacga agaaacaata tcattattcc tggattccgt tgacgatagg atttaccggg
     6541 ctagtgcagt cacacctgct gacgggagaa caggcaggcg ctttcactat tctgttgtgt
     6601 ctgatcctga tcaaaaaggt attccgcaag gagacgttct gttctctggc aaaaacagtg
     6661 atctacagtg tattgctgag tgcctggttc ctagtgcctt ttgctgatta tatgctgcgg
     6721 ggcgattttg tgattcagca tgtctctggc agagtgatcc agtatagggg actgtatctg
     6781 accaacctat tatttacttt tttccggaac ggctacaatg tgttctattc cgacaacgga
     6841 atggcagata gtcagcccat gggaatggga atcgcactga ctgtggcact gttactttgg
     6901 gcgggaatgc tctttttccg caaaaccacg cacctgcgca gggaagaaaa gacgttgggc
     6961 aagattgccg gaggttttgc cgttctggct atgtggatgt gtctgtcaat ctttccatgg
     7021 gataagattc atagcctgaa cgatattacc gcaacgctgg tgtcaagtat tcaatttccc
     7081 aaccgttttc tgaccattgc aacactgtgt gcagtactgg tggcaggtgt tgtggccaaa
     7141 gagatcaagg gacagtatga gacgcacgga ttgcagttct attttgccgg aatgatggta
     7201 ctgactctgt gcagcagtat ttacctgatg gatgatatgc ttcagacggc tgacgattac
     7261 cggatctacg gagaggaagg aatgggaagc ggctatattg ccggagcgga atacctgccc
     7321 tacggagcgg atgcctccct gttctggccc catgatcctt atgccgcaga aatagtgaat
     7381 attacggact atcataagga tggaataaag atagacatgc attgtgagaa ccggggtgat
     7441 aagacggaaa cggtggaatt accgcttctg tattattatg gctaccgggc atacgacaag
     7501 actaccggcc aggaactgac gatcaccacc agtgataatt atgcggtgtg tgtggaagtt
     7561 ccggcaggct atgacggaac ggttcaggtg acattcagaa gcccctggta ctggagagtg
     7621 gcagaggctg tgagctggct cagcctgctg gggctgatcg caggggtgac actggagaag
     7681 agacgggaga ggaaagcttc atgaaaataa cagaaaaatt aaaattcaag ggaaatggta
     7741 ttttctggtg cctggccggt atttttctga caggagcact cccgctgctt acaaattatt
     7801 gtctgaattc ctcggaggtt ccttaccagc tggttcgtat tgaaaatatg aaagactatt
     7861 tgtcagcggg aatttttcag atggcaatgc cggtaaactg gcagtatgag catggtacgg
     7921 caggtttgga cggaaatctg ttctggctgc tgccggtgct tctgcgaatg acaggagctg
     7981 cactggaaag tgtttaccgg atgtttctga tatgtattta tgccgtgaca gtgtgctttt
     8041 cctacaagac actggaagaa ggatttcaaa ataaaaaaac agcggtgatc ggtacggcac
     8101 tgtattgctg ggcaccgtgg tatctggatg ccttgtatgg aagagccgcc attggggaag
     8161 cgttaggcta cgctttcctg ccggtgttat tactgtttct ggtgagggca gtcgggcgga
     8221 aaaaaggaaa actgccgcaa tggcttcttc tggccatagc gatttccttg ctgttacagt
     8281 cttcttttgc ggttctggaa gtgggaatgc ttctgttagt gttctgctgc gtatattatc
     8341 gcagacaatt gctggaaaaa gacggctggc tgacattggg aaaggcagta gcggcaacac
     8401 tgttgtgcaa tttgtggtat ctgctgccga tgctaagata tcttgtggca tatcgtgatg
     8461 tggcaagata tgtgggaagc cgtccatttc aggaaaaggg agcttttctt ctgcattatc
     8521 tgaccttctt tttccaggga ggtgccacct atgattataa gagcaatggg tttcagggaa
     8581 cggctgccat tggtgtcggt ccggcactta cgggactctt ctttttcctg ctgtggctga
     8641 aattcagtgg ggcgctgcag aagaaaaaca gtaaagaaga cagcactgcg ggattgttct
     8701 ggtgcggaat gctgggaata cttctcagtc ttggcagctt tccctgggat ctgctccggc
     8761 agaatgccgt gttccaggtg ctgaccttca gtatgcagtc tcctgcgatc tttatgatcc
     8821 cggcaatctg cggactgaca tttataggat gcggtctcct gaacgcttac cgggagggaa
     8881 aatccgcaac agaagccacc atcctggaga cagcggtact tgcggcagct tttgtgtcga
     8941 cacaatttct gaccaataaa ttgctgttag gcagagcacc tttgtggatc cgccagaagg
     9001 aagatctgcc ggatgtggtt ctgatcgaag caccgcagtt tcagatgagt gccgcagccg
     9061 gtgtagcaca actggtagca gtggcgctca gcgctcttgg catagttggg atcgttatat
     9121 actgtatact cacacagaag aaaaatccgg accggaaagt acaaaaggag gcagcgtagg
     9181 aaatggatat gtggaagaaa tctgaaatcc tgaaaaacaa aaaattccgt attctgttgc
     9241 ttctggaagt tctgttactg ctggtgcctg tgatcggaat tctgtgcggc aataaaacaa
     9301 tcagcactct ggcagatgca caggtgacaa cggaagaggg tgtcatccgg gaagcaaaag
     9361 gttattccgt ggacggcagc agcaatatca gcggtccctg gctggaaatg agcggttttt
     9421 ccatggcacc cggtacttat tgctattatg tgacgtatga cacggatgac aacggcgtaa
     9481 ataagattaa cattacaagt gatggagaaa tctacagaga attgctggaa aacgagcttt
     9541 ccctgtattc cgggagaaca gaaagctcct gtgagatcta tgtgacaagc cgtctgaaag
     9601 cggatgaagt aaacatccac gcagactact acggaaccgg aaagcttact attaaggata
     9661 tccggctggt gaaaaccact gcgtcctaca gaatgctgct tgtgatgttc ctcctgggat
     9721 ttttgctgct ggattcgctg atcatgttgt atgtgtatat gggaaaatat cctcttgcgc
     9781 tggagaagaa actggtatac ttcggaattc ccgcattggc ggtcctggct tccctaccgg
     9841 tatttgtgga ttatattatg gtgggagccg atttgggatt tcacctgctt cgtattgaat
     9901 tcctggcgca gagcctggca cagggaatct ttcctgcgag agtagagccc atgtggctgt
     9961 atgggcatgg ctatgccaac tcactgtttt actgcgatac atttctggta attccggcaa
    10021 tacttcggct gatcggtttc cccatgagtg tttcctacga tggatatatc tttctggtca
    10081 atctggctac ggcggtcata gcgtatatct gcttctgcgg aattttccgt aaccgtgtga
    10141 ccggaatgtt cggaagtatg ttgtatacgt tggcaccgta tcgcgtatac aatatttata
    10201 accggagtgc ggtaggtgag tacacagcaa tgatcttcct gccgttgctg tgctatggtt
    10261 tttatcggat cttcacggaa gatatggaga aaaaggagta ccgccattcc tggctgctgc
    10321 cggtattggg cttttccggt attatccaga gccatgtatt aagctgtgag attgcgggtg
    10381 tgtttaccat ccttttgtgc ttattatgta tccgaaaagt attccgcaaa agaactttcc
    10441 tggaactggt aaaggtggtt gtgggaacgg tgctggcaaa tatctggttc ctgcttccga
    10501 tgttagatat gatgctggcg gatcaatacc gttattccaa taacagcggc gtatacattc
    10561 aggatcgggg aattttgggg gcacagatat ttttcaccat gcagaatgcc ggaagcaatt
    10621 ccagatttca ggagctggga atggtggaca cagagcctat ttatataggt gttgccgtat
    10681 tactgggagt gattgtgtat tttgctatcc gtaacaggga aaaggaacag gatccggcac
    10741 atgataaggc agccaaggtt gcatttattc tgggatgtgt ggcaattgcg gtaagtactt
    10801 actatttccc ctggaatgcg ctgaaggaag ccaattcctt gctggagctt ctgactacga
    10861 tgatccagtt tcctactaga ttgacgacca tcgcagcaat tgccatgact ctggtggcct
    10921 gcacggcggg acactggatg ctccggtgga aggataaggt ggcaaagacc atattcctgg
    10981 tggcagtctg cggcggctgt atctttttct ctatgtatca gactaacgat ctgctgcaga
    11041 cgaaagatca gccgttgaga ttgaattcta tggcatccat gggacattcc ggcattctgg
    11101 gagcggagta cctgcctttg gatgtggagt ggaattttta ttaccatgat gcatggccgt
    11161 cggacggagt gacagtggaa gcttttgaga aggaaaatct gaacacgctg actacggtga
    11221 cgactgttgc gggtcccgga gagtattata tcgatctgcc gatgcttttg tacaaagggt
    11281 atcatgccaa ggatatgaca acggggaaaa aattcccggt gactgtaggt gagaacggtc
    11341 atgtccgggc aatcctgccg gcgggctatc agggaactgt caaagtgtgg tattccggca
    11401 tgtggtattg gcgtgtggcg gaaggagtca gtctgctgtt ctgggtggct gtgaccgcct
    11461 atgaaatcat ctcgcataag aaacagcggg aaagggaata gctgccaaac tgattttttg
    11521 ttgaaaacag aaaatggaat atagaaaaag aagcagaaaa agaggatgcg ggtatgcccc
    11581 ggagaacgtt tgcataaagt agtagcgaat agttctgaga ggagatacca catgaaacgt
    11641 tttttcacgg aacacgaaaa aacactttgg ctcctgcttc tttttgcatg tatgtttttt
    11701 ctgggaagag ggattggagc cattgtatac ggactgcata tgcaacagga aatgagcccc
    11761 cgggaaacag tgggaagtgc attggagaat acgcaggagg tactttcagc agagaactgg
    11821 ggactgggat tcggaacgga aggaacgcag cccaccggca cggcatctgc ggaagaacta
    11881 aaagagtaca atgcctatta tgtgggagat gcggaggaga aaaagatcta tctcactttt
    11941 gactgcggct atgagaatgg taatagttcc gctatattgg atgcattgaa aaggcacaat
    12001 gcaccggcaa ccttttttgt ggtaggacat tttctggagt cggcgccgga gatggtgaaa
    12061 cgcatggtgg aggacggaca tacggtggga aaccacacct atcatcatta tgacatgtcg
    12121 aaaatttctg attcggcggt tttccggaaa gagatggatg acgtgagaac tctttttcag
    12181 gagaccaccg gtacggagat ggcgatgtac taccggccgc cccagggaaa atacagtgag
    12241 acgaatctgc agatggcaaa ggatctgggg tatgccactt ttttctggag cctggcttat
    12301 gtggactgga acgtggatgc ccagcccagc tacgaggaag ctttttccaa actgacaggc
    12361 aggatccacc ccggagcggt ggtgctgtta cataatacct cgaagaccaa tggggagatt
    12421 ctggatgaac tgctcacaaa atgggaagaa atgggttata ccttcggaaa attggaagaa
    12481 ctggtgcagt agggtttgac ttcccctgcc ccatacccta taatagagga cagaaaaata
    12541 tactggagga caaccatgga aaaagaactg gtagttgtca ttgattttgg cggacagtac
    12601 aatcagctgg tcgccagaag agtcagagaa tgcaatgtat attgcgagat ctactcttac
    12661 aagacagact tgaataagat aaaagcgatg aatcccaagg gaatcatcct gaccggcggc
    12721 cctgcaagct gctatgaacc cggtgcagct acctgctccg aggatctgtt taacttagga
    12781 atccctgtat tgggactctg ctacggcgca cagctgatga cccatgtgct gggtggtaag
    12841 gtagagcgtg cagccgttcg ggaatacggt aagactgagg tgaaggtaga ccgcagttcc
    12901 aagctgtttt ccgatgtatc cgagaatacg atctgctgga tgagccattt cgactatatt
    12961 tcgaaactgg cacccggttt ccgtgccgtg gccaccaccg ccaactgccc cgtggcagca
    13021 gcagagtgtg tagagcgcgg actgtatgca atccagttcc atccggaagt acttcacacc
    13081 gtggaaggct ccaagatgct gtataatttc gtacgcaaca tctgtggatg ctgcggtgac
    13141 tggaagatgg attcctttgt tgaagagtcc atcaaggcga tccgcgagaa agtcggcaat
    13201 ggtaaagttc tgtgcgcatt gtccggtggt gtggattcct ccgtggcagc agtactgtta
    13261 tccaaggcag tcggcgagca gttgacctgt gtctttgtag atcacggcct gctccgtaag
    13321 aacgagggcg acgaggtaga agcagtcttc ggtcctaacg gcaattacaa tctgcacttc
    13381 atccgtgtga atgcgcagga gcgtttctac agcaagctgg caggtgtatc cgagccggag
    13441 cataagagaa agatcatcgg cgaagagttc atccgtgtat tcgaggaaga ggcgaagaag
    13501 atcggtgccg tggatttcct ggtacaggga accatctatc ccgatgtggt agagagcgga
    13561 ctgggcggtg aatccgcagt gatcaagtcc caccacaacg taggcggcct gcccgactat
    13621 gtagatttca aggagatcat cgagcctctg cgcaacctgt tcaaggatga ggttcgcaag
    13681 gtaggactgg agcttggcat tccggagcat ctggtattcc gtcagccgtt ccccggtccc
    13741 ggactgggta tccgtatcat cggcgaagtg accgctgaga aagtcaagat cgtacaggat
    13801 gcagatgcca tctaccgcga ggaaatcgct aacgcaggtc tggaccgcga gatcaaccag
    13861 tacttcgcag ccctgaccaa tatgagatcc gtaggcgtta tgggtgatga gagaacctat
    13921 gattacgc
//
LOCUS       DAWDHD010000060        13881 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_295_length_13881_cov_12.482641, whole genome shotgun sequence.
ACCESSION   DAWDHD010000060 DAWDHD010000000
VERSION     DAWDHD010000060.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 13881)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13881)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13881
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_295_length_13881_cov_12.482641"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3696 a         3774 c         2830 g         3581 t
ORIGIN      
        1 taattgtata cagtgatacg atttattata tacaagaaat ggcttatcgt caacaaatta
       61 gtttgctatc gctgtaatgt gcgaaagtat ataactgtgc tcatgtgtat cttattacaa
      121 ctttctgtca aaacacacgc gaagcaattc atagaacacg taggctgcca gccccatcag
      181 gaagtctgat tcatacagat ataccaacac agtaaaaatc gtccatattt ccgccaatgg
      241 atctgtcagt tgcattgctc ccatcagagc atgtgtcccg ctaaagtaaa aatatccgtc
      301 agaaagcagc atcgacaagc gagtatccag tcttgcataa caggctccga tgaccgcccc
      361 tgtcagcgca gccccctgca aaaggaacgt tcccgcatac atccgaagac attgtaatac
      421 agatttttct ctccggatcc ggaaatctct gccatggatc agcagacaca gcagcagtgt
      481 aatcagaagc acccggaaga ttcccaagcc ataagggaag gtaaacagaa agctctccgg
      541 catagcttcc caattccatg tttggtttag caattcctcg taagtcactt ttgatgtaaa
      601 aatccagaat tccaacaatt tgctgaacat tagcaataac agatatgctg ctgccacacc
      661 ggcacatttt acagctgttt taactttatc cattcttatc cctccgccat tttttctgtc
      721 ggatccgtca ttgcagctta ccatgactgc tttatcatgg taagctacac tctataagtt
      781 acgcctttat aataacagcc ggaacagtct ttgtttcttt ttgtccccga atgactgttt
      841 tttgaccttg aaatttgact ttctgacaat tacagcctct gaggttaagt tttcacaacg
      901 aagcttacag gttcaaccag acacgtaccc ggaattcttc cgtctcttcc actgtttcat
      961 aatcaatctg cccctcatat tttccaacga tctgttccac gatccgcagt ccccagccat
     1021 gcttcgcact atcctgtttt ttcgtccgaa gatgtccttc cgaaatctcc ggtttctctc
     1081 tgaaatcatt cctgatttcc aataagagca tccccggcac cgtccgggtg gcaatatgta
     1141 ttctgctttt tgccgcctcc agtgcatttt ccgtcagatt ggcatacagg gcacagagat
     1201 cctttttctg caatctgtca ataccggcaa atagggatgc ctcgatctgt aagtcgatat
     1261 cttccgcttc agcctgctct ttcgcttcat ataatacagt atccaccagc tccattccgg
     1321 tatataccgg caccgctgct gtccggaccg gttcctgcac accggcaatg tatttcaaag
     1381 cctcggcatc ttcccctctt gtgaccatct gctccaccgc ccggagatga tgatgcatat
     1441 cgtgatacag cttcgcattt tccgcataaa aatcatatgc cttccggtag ccctgctcca
     1501 gaattccgtt ttgcagatcc agatactcca gctgctctcg catccgcgtc ttctgtgcat
     1561 aaaaataagc tgccgccaga accgttgtca ggcaggaaaa ataaaaatat tctttcattc
     1621 gttctccccg cagcatatcc cagagaaaat tgccgaaaaa caacgtcagg aaaaaagccg
     1681 ctcccatggt tccctgtatg accggaaact gccttctgta tttcctcacc gtgtcggatt
     1741 gtaagaccag atttcccatt aagttcacct caaaccatgc cgcctgtccg atcagcactc
     1801 cgtatgccga cagcctgccg gcgaaccaca acgttacctc cagtcccagc agataacctg
     1861 cggcatacag aaaacactcc ttctcttcgc attcccacaa aagaccgaag gagacggtaa
     1921 aaaggagtaa tgccggaagg caggacagca tctccctcat accataccat aagaatccat
     1981 atccaagggt cagacacagc actcgcttat ttcccataag aagcttattt tccctgccat
     2041 cactcccata taaaccatgg gcatgatccc gttccatcca ccataccatc caaaatgctt
     2101 cgtgaaagct acacaaaaaa gttatcatct gcatttgtgt cacgtctgtc atatcttttt
     2161 ctcccggatc caatactcca tgactttatc ctttaattcc cgcattctgg ccgaactgac
     2221 atagagttct tcccctgttg tcagcgtcag tttatcgttt ttcaactgtg tcacctttgt
     2281 cacattacac aagtatcctc tgtcaatttg aaaaaattgt tcctccggca gctcctccga
     2341 cagacttttt aatgttctcc gtactctgtt ccggcttccg tccgccttcg caatgcaggc
     2401 gtatttcccc cgctgccgga tatatgtgat tgcttccaac ggtattttct ctgctccctg
     2461 cccgttctct accagcagga accgattctc tctgcgtttc agttccgcca cagctgccga
     2521 aagagtctcc gaaagcatct gtgtcaggca tcttttgggc agaaaccgaa agggctgtaa
     2581 tttaaaagaa tcgtagacat acttttcata ggaagaaaca aaaataagaa gtgacgccgg
     2641 aagactgctc tgcagccgct ttgccaacag aaatccgtcc tcatccccca gctcaatatc
     2701 caaaaacagc aggtcaaagt cttgtccgtc ttctacttca tattccaatg cttttccgtt
     2761 ctgaaatata tgacagcaac cgggttctcc cattatttga agagactccg aaagtagtgt
     2821 tttcatcatt tgtccatctg ccctgttatc gtcacaaatc gcaatctgca gcatagtccc
     2881 ctcccgcctg tttttcgaaa taccttatta attacagcat aagccactgc caatacttct
     2941 gtcaatttac tttccaaagc taccgggaat ggggagaaaa ccgcaagtat tccttgaaaa
     3001 agtctcccgt ctattatatg ataagaaagt cagaagacac ggaaatccgt tgttttttca
     3061 gagagaatca tttgcttctg tattttataa tgtttattca aagaaaggtt tttaacatat
     3121 gtggattgca aataattgga aagattatga agtgctggat acgtccagcg gagagaaatt
     3181 ggagcgttgg ggagattaca ttctggtgcg tccggaccct caggtcatct ggaaaacgcc
     3241 ccacgaccat aaggcttgga aacaaaaaaa cggtcattac cacagaagtg cgaagggcgg
     3301 cggagaatgg gagttcttcc atctgccgga tgaatgggcg atccattaca ccctgccttt
     3361 tcagaaggaa gcctccggta agaccgatcc tctgaccttc catctgaagc ccttcagttt
     3421 caaacatacc ggtctgttcc cggagcaggc ggtgaactgg gactggttct ccggtctgat
     3481 ccatgaggca ggccgccccg tgaaggtact gaacctcttc gcttacaccg gcggtgccac
     3541 tgtatccgcc gcagctgcgg gagctgctgt gacccatgtg gatgcgtcca agggtatggt
     3601 cggctgggcg aaggagaacg ctgctgccag cgggcttgcc gatgctccca tccgctggct
     3661 ggtggacgac tgcatgaagt tcgtagagcg tgagatccgc cgtggcaaca aatacgacgg
     3721 catcatcatg gatcctcctt cctacggcag aggtcccaag ggcgagatct ggaaaataga
     3781 agataatatc tgggatttca taggactgac cgcacagctt ttatccgatg atgcgctgtt
     3841 cttcctgatc aattcgtaca ctacaggatt gcagcctgcc gtgctgtcct atatgataaa
     3901 ttccaccctg acgaaaaagt ttggcggtca tgtggaagcc gaagagatcg gcctccccgt
     3961 caccgagagc ggtctggtac tgccctgcgg tgcttccggg cgctggcagc ggaagtaatc
     4021 agactagcat cataagccaa atcaagaagg agcctctggt cgaggctcct tcttgatcac
     4081 agccaaagtc agcaactatc gcctgatgat tgaattccaa atatggtgcg tttttactcc
     4141 tctgtttcaa tctccaatct ctgaagaatg tctctcacaa taccagcatc ctcaatcaga
     4201 gaaattccaa aacacttttt tcctgcatct tttaaggagg ctcctatatg ataaacattt
     4261 ttcttatcca agattaaaaa gcgatcatga aatactttcg tgtaccgcac ttccagtaaa
     4321 ggatactggc tgttgaaatt attaacatct tcttgagata acttggttct tttcaatgta
     4381 tacatcacaa cagtcacatt ttcattcttt tttgtgagaa gattcagcgt tcctatatcc
     4441 acatagccat caattaaaac gatttccttt tccgcttttt gaatcaggct aacaattagg
     4501 ctaaaagcat catatatctg tccatcaaaa aagactttct gatttgattc ctcatggtcc
     4561 gatatatatt caaatatctg atccaatttt tcatccgttt gcttttgata ttccagttgt
     4621 tttaactcca cattactgat acgctcaaaa agcaaggcgt tattagcaat aaatcgcctc
     4681 atttccacga atgcgcgcat aataccaatg cttaccttaa ttgccacttc gctatgtaga
     4741 acactagcca acatagagat tccctgttct gtaaatacat aaggaagagt cctacgacca
     4801 ccataattat tttcgccctg cgcaaaactt gaaatgccag tttggcatct caagttctga
     4861 tactcctctt tcgtgagctg aaaacgaaaa tcttctggaa atctactaat atttcttttt
     4921 actgctctat tcaatgcccc tgtctctacc tgataaagaa tggcaagatc actatccagc
     4981 atgacttgct tatctctaat aacataaatc attttttcaa tcgacggtcg agtgatttca
     5041 actaaatcat ttgtaatacc atccgtcttt ttcgcttctg gtttaatttc tatcataaat
     5101 tctccctata acatttacac gatatcccga taccgtacct tgccattatg tttggaatgc
     5161 ctttcccata gaattccttc ctgtgtttgt tataaccgca ggatcagaca ttccatattt
     5221 atgagattat agtagcaaaa acagacatac ctatgtccca tacaaagtac gtatatcgca
     5281 gtcaaaaccc cgctgttatt ttagaatgtt gtaaatttgg aataaaagca tggactttcc
     5341 gactcgctat tcagaacttc acaagatatc tgtagaaata gaaaacttct atgctttgtg
     5401 cacattctag catagaagtc ttctgctggc aaggggtatg ctattatcaa atatttgtaa
     5461 tgcaaaacca gatttgccgc ctatatttca cttctgatca caacacgaat tttcttaaca
     5521 ccgggatctt gcgcagcacc cacgcagtag ccgttgccag tgccagcact accaggcaga
     5581 atacagccag cagcaaaatc aataccgctt gtcccgcggc tacagactgc acaccgcatt
     5641 gttccaacac cgtaaagggt gtgatcttca cgaatttata caacaggttc acatacaccg
     5701 gatgtaccag gtatacggca aaactcaagg ctcccgcctc ctgccaggga atccggtgta
     5761 ttctgttctt ctcccgggca gccaaagccg cgctgtctcc tcccccattc catcctgcag
     5821 caaacagaag cactgcaagc agtactgtaa acggataatt gtatgccatc tgaatggaga
     5881 atccggcgag tgtcctgctg aggatcattc ccagagccag aaccgctact gcagctgtca
     5941 gccatacatt ccttcccttg tccacaccta cttctctcac cgcgaagaga tatccacaca
     6001 gataatacag gaaatacaca ccgccatccg gcaataccgg aatactgttg acgtccagta
     6061 ccttattcag aaacggtgcc acactgcttc ctaagaagat cactgccatc actgccgcaa
     6121 caccccacac cggcagcaca cgcagcactt ttttcagaag cggcgtgatc agatataaaa
     6181 acaggatcag atacagatac cacatatggg accaggtatg tcccgtcagg gtcatcttca
     6241 gtgcctccgg gatcatcctg acccggaagg tccgctccgc caccactagc tcggaagctg
     6301 catacggaac accgaatagg aggatcgcca gcgcaatcct cctgcaatat tttttaatca
     6361 tgaccggata agtcaatgtg cgtcccggat tcaggaacaa atatcccgag atcaataaaa
     6421 atataggaac acaccaggtc accagatcca taactgacag caataatgac cggtattccg
     6481 gcactatcga cgcatctgtc acatctgtcg cacctgtcag tacatgcatc agcaccactg
     6541 cacaggttgc cagtacccgg agcacgtcca ggaactgctt cctgggtccg gataagccgt
     6601 ttgtcatgtc tctcccatct cctccatcag gcgcatgcct gcagtatctg tcacaggaag
     6661 ggagaatact atgctttgtg ctttactctc cagtcctgct ttttccatta tagcacgcat
     6721 gatggaattt ttctgctcac tttttaccac aatgaacaac atttcttttt ccggcaccag
     6781 tgtcacgccc agaaactgct gtgcattctc tgttccggtg cccttggcat ggatgactgt
     6841 gccgcctgcc gcatgtactt ctctcgctgc atccatgacc agttccgtat atccatgatt
     6901 ggctatcact accagaagct cattcttcgt ctctttcaat gtggactcct ctccttttac
     6961 gaattcctgc ccgcaggtca ggtaattcaa tgctttcttg ccaccgacac tgctcacggg
     7021 aatgataaat gcgattccca ttcccgggag atcgatctgc attttcttac gcagagcagc
     7081 cttgatctgc cgccaggcag catctgtgac gagatggaac aatacgctct tctccatgcc
     7141 gtccagtcca aaataatcta agatctcatt cgccgcggtt cccatgccga cggtaacact
     7201 gccgacctgt gcgccttttt ctttatagca ttccacgaat ttcctcgtca gctgacgatt
     7261 ggtgatggtg accatcatat ataattcact catgggcaca cctcctacag ttcaatgatg
     7321 gcattctcat ccaaagcacc aaatgcatcc gccgcaccgg caaatacgcc tgcaccgctc
     7381 tttggctgct ttaactgata taccattccc aatatctgga tcgtgatcag cggtgtcatg
     7441 gctaccattg ctaccacacc gaaagcatct gcgactaaat tgccgcccac cgccacacag
     7501 gcgccctgcg ctaacggcag taaaaaggta gctgtcatgg gaccgctcgc cacaccgccg
     7561 gagtcgaagg cgatcgctgt gaatatcttc ggtacaaaaa aggatagtcc gatcgccaca
     7621 ccatagcccg ggatcaaaaa ccatagaatc gggatccccg tcagcacgcg gatcatcgcc
     7681 agtgctacgg ataaggatac tcccacggac agagaaaccc ccatggatct ggcagagatg
     7741 gcaccgtccg tcagctcctc tacctgatga ttcagtacat agaccgcggg ctccgctttt
     7801 acgataaagt aaccgatcag tgctcccacc ggcaccagta cccacctgta ggatctggct
     7861 gccatgacct gaccgaggta attaccggca ggcataaagc ccacatttgc accggtcagg
     7921 aacagtacca gaccgatata agtatacacc agaccgatca ggatcttcgc cagtgtcttc
     7981 cccgacagcc gcagggcaaa tatctggaac agaccgaaaa acaatacaat gggaagtaat
     8041 gagagtgcga tctctttcat ataatccgga atcccatgag caaaaagttg cgccagttcc
     8101 acggaatcag cgatctccgg gatcgccacc ggctggtaat ccgcattggt cggcttatag
     8161 atcagcccca gcaccatgac cgccaggatc gggccgatgg agcacagtgc taccagaccg
     8221 aagctgtcgt ccgccgcatg gtggtcatta cggatggacg caataccgac acctagagcc
     8281 atgataaaag gaactgtcat gggtcccgtg gtcacacctc cggaatcaaa cgccactgcc
     8341 cggaagttct ccggtacgag aaatgcaaga acgaacactg ctatgtagaa aaaggttaat
     8401 aacggcggca gtgccacacc gatcagcata cgcagaaggg caatgaccag gaagattcca
     8461 acccccactg ccactgccaa aatcagcgtc atgttcggca ccgaaggcac ctgtgtcgcc
     8521 agcacctgta agtcaggctc agaaatggtg atcaccactc ccaacagaaa agataacact
     8581 acgatcagca gaatattttt actctgggtc attctggcac ccactttttc tcccatggga
     8641 gtcatagaca tctccgcacc cagagtaaaa aacatcatac cggccatgac catcaccgct
     8701 cccaccagaa aacacaacag gatactggag gttatgggag ctatggtaaa gcttaatacg
     8761 atcacgatcc ctactaccgg caggaccgcc ttcaaggatt ctgcaaattt ttgccggatc
     8821 tttggattta ataacttcaa tattggtctt tcctttcttt atgttttctt tatcttaaca
     8881 tgaagcaaag acccgatcct accgtttttc gtgaatttat aaaattttca cggggtctta
     8941 acgcaatctt tatgcagaca gctcccgatc ctgcccagac tgctttttca caaatttata
     9001 ggcccagaca tccaccacga tcacaaacag gaaaaagaaa acgaagatcc agtccgcatc
     9061 cagagacaat gcggaatcat agataccgtg ttcgatcatc ggcatgaaca cgctgagctt
     9121 caggtagcat ttcctgcgtt ttttattgcc tgcaagttct gcctccttcg ccacaccgaa
     9181 gaaatatccc atgatcacgc catcgatggc atgggaaggc acggacaaca gggctctggt
     9241 cactgccgtc ccaaagtcac cgcccagaca atacagaaca ttttcaatgg ctgcaaatcc
     9301 taatgccgaa gtcacgcagt acacgatggc atcgaattta taattaaatg ccggatgctt
     9361 ccatgccgcc catttggccg cctgcatctt gcaaagttcc tcgatcagtg ccaccccgat
     9421 aaaattatcc aggatcacgt agcacagcgt atctgcatcc ataaatacca gaaatacttc
     9481 atccagaatg atttccagta tcatggcggg gatcacagac aatacaccaa acaggaatat
     9541 tttccacaat aatccccccg gctccttctc cgccttatcc tgtctataga tccataccac
     9601 cagtgcgatg gccggcaata gtgccaatcc tgtcagaaac ataacctatt cctctcctgt
     9661 ccacatagac ggtccgccct ttcaggaccg ccgccatact acattttaat ctaatgtttt
     9721 tcctgatgtc aacctaacga aagtatatgt tcctattttt tgtgtcttgg attttcctga
     9781 tgctgcctgc cttttgccgc agctttaggg gaaaatatac cggttttcat atatccttaa
     9841 ctacttcttt acaccttgca ttgcaaagta atggtactat agttataata aatttacaaa
     9901 acaactaaca atatttcatg gaggatcaca ccgtgttccg atcactttcc gataaaaaat
     9961 caccgcggaa aagcttccgc ttaaaaccac cctttaccag acatgccatc agtatcctga
    10021 tctgtgccgc ctgcatgctg ttcgccactc ttgcctcttt tttatatcag catatagtac
    10081 cggacaacca tgccaatgta gcactgttct acattctggc actggtgatc atcgccagat
    10141 ggacggtggg ctacggctac ggcattgcct gcaccctttt ttccgtaata gcgatcaatt
    10201 atctgtttac ctatccgtat tataagctta atttcacact gaccggctac cctatcacct
    10261 tcctgctgat gagcgtgatc actctgatcc tgtgtaccat gacctcccat atgaccatcc
    10321 agtccgagat gatcgcagag cgggaaaaac ggctggcaga ggccgaaatg gaaaagatgc
    10381 gtgcaaatct actgcgcgcc atctcccacg atctgcgcac gccgctcacc ggcatcatcg
    10441 gcaacagctc cctgtttcta gaaagtcaga acgatctaag ctccaccgag cagcgcacga
    10501 tcatgaacaa catctacgag gattccaact ggctgttgaa tatggtggaa aatctgctgt
    10561 ccgtcactcg tatccaggga gacagcttaa gcatcaatac cacagaggaa ccggtggagg
    10621 aagtggtggg agaagctttg gaaaagctga aaaagcgcta tcccgatgcc gccatccggg
    10681 tcaagatccc cgaggaattt ctgatgatcc ccatggatgc ggtgctgatc gagcaggtca
    10741 ccatcaatct gctggaaaac gccatcgttc attccggaag catcctgccc attgatttca
    10801 tcgtggaaga tcatccggag catgtctctt ttattgtgag agactacgga aaaggattgt
    10861 cggaagaaaa attacagaat ctgttcgaga cgggcaccta caacaattcc cggagttcgg
    10921 attcccgcaa gggcatgggt atcggtctgt ccatctgcaa gaccatcatc accgcccatc
    10981 acggaacact gagtggcagg aatcatgcgg atggcgcgga attctgtttc acactgccca
    11041 aggtgggagc tgtcgcttca gaaaaataag tgtactctca gccacataag acagaactct
    11101 tgaaaatacc ggaaaggata tggatcacag atatgaataa gatcaaagtt ctcttaatcg
    11161 aagacgatag aagtatcagt aatttcatca ccgctaccat ggaccgggaa ggctacaaga
    11221 tcatgcatgc cttaaacggc agagagggac ttagtctcag cacttctttc tgccccaatg
    11281 tgatcctgtt agatttaggg ctgccggaca tggacggact ggaggtatta cagaagctcc
    11341 gttcctggag cgatgttccc gtgatcatca tatctgcccg caccaaggag caggaaaagg
    11401 tctccgctct ggatctgggt gcagacgatt atatcacgaa gcccttcggc accggcgagc
    11461 tcatggcaag gatccgcact tctctgcggc acagccacgc ccagactccc ggtcatgtat
    11521 ataaggcact ggatctggag attgatttcg agaaacgact gatccgcctg cggggcgaag
    11581 aggtacatct gacccagatc gaataccagc tgcttacact tttagcggaa aattccggca
    11641 gagttctgac ctacagctat atcatgaatg ccatctgggg cccctacatg gacaataaca
    11701 accagatcct tagagtcaac atggccaaca tccgcaggaa gctggaacag aatcctgccc
    11761 agccccagta tgtcttcact gagattggtg tgggctatcg gatgcgtgag aacgagaacg
    11821 gtgggactgt ataaacagaa atacaattcc caacactgcc tctagcaaaa tgagtaccgt
    11881 ccctccgtcc agtgccttcc agacgctgtc cagggtatcc atccggccca ggatgtctat
    11941 gtaaaagaaa tccagataga tgaccgccgg tgagatcaca catcccggat gcagtaccgt
    12001 ccgctccatg agcagacggt acatttccac tgccagaaat attacattca gggcatagcc
    12061 cgcacaaagc tgattgttca aaaacggtcc cagggagctg agttccatac caaggggaac
    12121 acttcctgcc cggaccattc cgaagcttaa atacatgatt ccaacactcc ataatagcat
    12181 cgcaagggat gctattccaa gtcctctgct taattgactg gagggaatca ttttcacatc
    12241 ctcttccttt ccgaaatacc ggtatacaag ccccagcatc gtcagcgtcc ccgccatgga
    12301 gattccggcc accaggcagg caatatctct tcccccaaac aaccctcttc cggaaaataa
    12361 acggatcccg atgacacagt gatagatcaa aagccccagc acgatcagat acaggatcct
    12421 cttttcacgt ttccgctgct cctggagctt tgccagtctc acgacttccg tcacagcctc
    12481 ctcccggtct cccgcccgct ctccggtcac aatgtcctga atcggcagtt ccaacagatg
    12541 agacagggac tccaatacgc cgatatccgg aaaactttct ccattctccc accgggatac
    12601 cgctttgttg gtcaccccta acatccttcc cagttcactc tgggtataat ttttgcattg
    12661 tctggcttcc cggatcagat tgcccgtttt ctttttatcc atactaatcc ctataccttt
    12721 ctgtactctg caaactctag gtgctttagc acctttgctt tagcaccttt gctttagcac
    12781 cgtatgccgg cctatctgta cccttatggt aacaatcctt tggaaaatgc gcaatccatt
    12841 gtaacgagac tgcctgtttt ctgtgatttt tgcgcaaaaa attcccctcg gcgagggggg
    12901 agtgtaccga gggggataga gagaaaagta tttttgaaag aggcgggttc ctttttcagt
    12961 tattataata gcgtaattta tttatattta ctgctgtttt tatagcttgt ttgtgttgaa
    13021 aacactgttt ttttgtggta ttctgactgt atcaatgtca gtagatttaa aaagtgaagg
    13081 agcagctatg gatatcacaa cgaaaaaagg cagacgcggc agaaaggata ttgaccggat
    13141 catcgtggaa aaaggccgca gaggtgattt ttctcccctg cagcccgatc attatcacag
    13201 cttttatgaa attttttatc tctggtcggg aagctgccgt ttcctgttaa aagatacggt
    13261 ctatcagatg gaagccggag atctggtctt catcgctccg ggagaactgc atcactctct
    13321 gtattccgcc ggagaggtct gcgagatcgt aatgatctat ttcaaagaag aatatctgca
    13381 tttatcggac cggtctgccg acagcctcac ttccatatcc gggcaggggc tacagctgca
    13441 ttccttcatg ggcagcgttc ctacggtata ccaggaatat ctgcactctc tgctgacccg
    13501 gatgctcacc gagaattccc gcttcgacca atactccgaa cattttgagc gttgctatct
    13561 ggaagagctt ctgctcatgc tgatgcggca ttccgtcatg aatgaaaagg aaccggatct
    13621 gctgaactcc cgggatgcgg acatcctgct tgccacaaga tacatttaca acaacttccg
    13681 caagcccctg acactggagg aagtctcctc cgtggcttcc ctaagcccca cttatttcag
    13741 caagaaattc aaattgatca ccggcatggg cttcaaggaa tatttaaact atgtacggct
    13801 caaacacgcc cagacggcgc ttcttaccac caacagttcc atcacggata ttgctctgga
    13861 atacggcttc aacgacagca a
//
LOCUS       DAWDHD010000061        13848 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_296_length_13848_cov_12.749873, whole genome shotgun sequence.
ACCESSION   DAWDHD010000061 DAWDHD010000000
VERSION     DAWDHD010000061.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 13848)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13848)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13848
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_296_length_13848_cov_12.749873"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3912 a         3037 c         3697 g         3202 t
ORIGIN      
        1 cacatccgga gaccttacat cgagacatgt gtatcgcatg ctccgggagt tcgtctggtt
       61 taaggctccc tccgctacga accatgccta ttcataagat tgtcagcaaa gaacgctgtc
      121 aatcttattt catagacggc tgtcgccgta taaaaataga gtgaaccagc ctcgcttgtg
      181 cgcaagcgcc catcgaggtg ttccactcta tttttgcatg gctcgtagct aggctaactt
      241 tctatttatt caacagatac tccgccagat ccaccgcggt ctgcacatgt tccgcattgg
      301 catagatgcc catgacgatg ccgtcaccac ggaagtaata agtatcagtc agatccttgc
      361 agtctgtgag gcagatgcct accggtacgg gatcctgcat gtcctccggg tggaggggat
      421 cgggatagtc tggggtataa tcggtattca tatcatcgtt ggctgcttcg atggccagta
      481 ccacttctct gtctacataa tagaaataag gttccagtgc ctgtatctgc tcatcagtga
      541 gaatatcccg cagatcatga aacatgctgc tgttggcata tttctggaaa gaagtgaaat
      601 ctgtgatcat acagtccaat tcattggctg ccacatacac catcagcttc tgggaagaag
      661 tgatagaagt ttcatccatg ctgtcttcca cgatacggat ggaagtatca aaagtgatat
      721 cgctggtgtt cagatcaatg ccctcttttt ctgcaaaatc cgtaacaaaa tccggctgtt
      781 ccgaggtcat ctgatctaag aggctggcat tcagcatcac ggcataaaaa gagggatcct
      841 tccgggctgt aagctgtttt acaaaactga cgatcataat gatcaccacc agggcaatga
      901 tcacatgcca cttgtaataa taccagaaat acgccagttt ctgctttggc gtaccgtttt
      961 tcaatgcctc tcgttcttct ttgaattcat ccattactgc catggggaga tccgcctttc
     1021 tgttgccgaa atatggcaga acgccacatt ccgggtaaat ttcttcacaa tatactaatg
     1081 ataaaggttc gctatctgaa agtcaatgaa cgaagtgtga ggggaatcta aaaaaacaat
     1141 taaatcgccg ggaaaatccg ggcatgcctt gcaagttttg tgaagatgat atatcataaa
     1201 agaaaacgat tgtcaggaga aaacagaatc cggacagccg taaacagaga tttcccaaag
     1261 aaacggaaga acggtgcggg caatttcaga accggcccgc aagagaaggc tttccttaag
     1321 agggaatagg ctctgcataa aataaacgaa gagaaaagga aacaaaaaat gagtgattct
     1381 tacgtagaat gtctggtaaa agcaaaacag cccacctggg caaaaatttt aaaggtatta
     1441 ttgatcgcac tgacagtact gagctgtctg gtaatgttcg tgtttatcat tttcctgccg
     1501 gtggcactgg cagcgggggt gggagcttat ttcctgaata tgtataccga tctggaatat
     1561 gaatacctct atctggacaa ggaactgtcc gtggacaaga tcatggctaa gagcaagaga
     1621 aagcgtgtcg gtacttacag ccttgaccgc atggaggtat ttgcaccggt ttactcctat
     1681 catctggata acttcaaaaa ccgtcaggtg aaggaaaagg attatagtat cggcgaggta
     1741 ttgcagccgg acgggcgtta tgctatgtac tatgaaggtg gcgagaagat cctcttaagc
     1801 cctaatgagg agctggtcaa ggtgctcaaa aacatggcac cccgcaaagt gttcagcgaa
     1861 taatatttcg caacagtccg gtgcagggaa cagcatcaaa gcagttgaat gcctggcgag
     1921 tcaaaaatat aggtctggtg tacatgatac gccggacctt ctttattgca gaaacagaaa
     1981 taaacgcggt aatatgattg ctaaatgata aaaaatataa gatatccggt tgacagtcct
     2041 acgaaactct gctaaactca tggaaatgtt ataagtacaa cgaatatgta atataatcac
     2101 aacttataac acaacaagat atcattcaca aatcttatca aatgaacaca ggaggaaaga
     2161 aaatgggaca gattattggt gaaggcatta cctttgatga cgtactgctc gtaccgtctt
     2221 actctcaggt agtccctaat caggtggatt tgaccacatg gctgacaaag aagatcaagc
     2281 tgaacattcc tatgatgagc gcaggaatgg atactgttac cgaacatcgt atggcgattg
     2341 ccatggctag acagggtggt atcggtatca ttcacaagaa tatgtctatc gaggcacagg
     2401 cagaagaggt agataaggta aagcgttccg agaatggtgt catcaccgat ccgttttctc
     2461 tgtctcctga acatactctg gcagatgcag atgctctgat ggctaagttc cgtatctccg
     2521 gcgtacctat caccgagggc agaaaattag tcggcatcat caccaaccgt gatctgaagt
     2581 tcgagaccga cttcaccaag aagatcaaag agtccatgac cagcgaaggc ctgatcaccg
     2641 ctcccgaagg tatcactctg gaagaggcaa agaagatcct ggccaaggca agaaaagaga
     2701 agctgcctat cgtagataag gacttcaact taaagggtct gatcaccatc aaagatattg
     2761 agaagaccat caaatatcct ctggcagcca aggattccca gggcagactg ctctgcggcg
     2821 ccggtgtagg tatcaccgcc aacgtactgg atcgtgtggc agctctggta gcagccaagg
     2881 ttgatgttat tgtactggat tccgcacatg gtcattccca gaatgtattg aactgcctga
     2941 agatgatcaa ggaaaaatat cccgatctgc aggtaatcgc aggtaacgtg gctaccggtg
     3001 atgctacccg tgccctgatc gaggctggcg cagatgcggt taaggtaggt atcggccccg
     3061 gttccatctg taccacccgt gtcgttgcag gtatcggtgt tcctcaggta tccgctatca
     3121 tggattgcta cagcgtggca aaggaatatg gtattcccat cattgctgat ggtggtatta
     3181 agtattccgg agatatcacc aaggctattg cagccggtgg taacgtatgt atgatgggtt
     3241 ccatgttcgc aggatgtgag gagagcccgg gagaatacga gctgttccag ggtagaaaat
     3301 acaaggtata ccgtggcatg ggttccatcg cagccatgga gaacggcagt aaggatcgtt
     3361 acttccagga gaatgcgaag aaactggttc ccgaaggtgt tgaaggccgt gtggcttaca
     3421 agggcagcgt ggaagatacc gtattccagc tgctgggcgg tctccgctcc ggtatgggtt
     3481 actgtggtgc acaggatatc gagactctga aggagaccgg cagattcgtg aagatctctg
     3541 cagcatccct gaaggagagc catcctcatg atatccatat caccaaggaa gctccgaact
     3601 acagcgtgga tgataaataa gttgtgaaaa taaggctata atgaaagaag cgattgtaag
     3661 tctgacttgc aaccgcctct ttttggaaaa agaaatcatt tgaatctcaa aatatttgca
     3721 ataattgaca aaaactttgc aatttttcaa gcaatataac gaatatggca aaaaatgtaa
     3781 aataatatct tgtatgaatt tcctgtcaca tggtaaaata tatttgttcg ggtgccgagg
     3841 aagggcgtat tccccgaata tttaaaagag aagcagggga gcaaaaaagt ggctgaaatt
     3901 tcatttcgag aattgatcga tcccagacgt ctgcagcatt tgcagaatga attctgcaag
     3961 gtgacaggcg tggcagctgt ctgcctggac agtgagaatc aggtgttgac ggagccgtat
     4021 gtggacaaga catggaagag accggatggg gaagatccga tcagtacgga atatcgcagg
     4081 aaagcagcac aggcactgga cagagtgcag gccggttcgc tggaggaaca ggtggtggaa
     4141 gaactgccgg acggcggaca tgtggcagca gtggctgtca gcgtggagaa caacacgatc
     4201 ctgtactggc aggtgtatga tctgaagaag atcgacacca tatcctttta tcagatattg
     4261 gatctgctgc gggataccag cgtagatatt tacagagatc gtatgtcctg cttcagcgcg
     4321 gaggcagaga gcagaaggag ccgttccgct gaggaagaga tgagtcggaa tctccatact
     4381 attgaggcga ccacggagat cgtccagctc ttagacagtg atgaacagat tgaacttgcc
     4441 atgaataagt ggctgaagat cctgtcagag catatccgtg tagatactgc ggatattttt
     4501 caactgcatt ccgacacaga taccatgaac gtggtctgtg aatggcgtgc cccgggacag
     4561 atttcttatt ttgacaagat aaacggagtg gaagtatatt ccttccttca tgctgagaaa
     4621 ccactggtgg tatccacgga cagcctgggc aatgccggct cgaaggaaat cgaggagatc
     4681 ggcatgaagg ccgtgatgat cttcccgatc ctgaagcagg agagcggaaa tatggtgctg
     4741 tctctgaatc atagaatcca ggggcatgtg tggagcatgg cggagatcaa gttcacagcg
     4801 gatgcagtga agatcctgca gagcatcctc accagaagga tccagaagaa ttccctggcc
     4861 ggttcctacg cagcattgga ggagattctg gacaacgtgg gctgtgccat ttatgtgaca
     4921 gaccagacca caggacggat gctgtttgcc aaccagatcc tgaaaaatac ctttgccaag
     4981 gaactgctgg atcacaattt tgatgctttg ttacagagca gtgtccgcaa agaaaaaacc
     5041 aagtcagtca gtgtagtcta ccatgcggag aaggagacct ggtacgatct gctgtgcaag
     5101 gagattgcct gggtggacgg taaaaaggcc aatctgtact ctctgtatga cattacagat
     5161 aaaaaactgt accaacgccg catcgagcag caggcctata cggatttcct gacgggactg
     5221 tataatcgta tgtgctgtga gagggatctg gcaagacaga tcgatcaggc gaagaagacc
     5281 ggcggcgaag gtgctctgct gtatctggat ctggatgatt tcaagcatat caacgacgga
     5341 ctggggcacc agtacggaga tgtgctgtta aaatccattt cccatgcatt aaaacggatc
     5401 aacggcatcg agaatacctg ttaccgcatg ggcggcgatg aatttgtcat cgtgatcccg
     5461 cccgacagct attcccggtt tgaaaatatt gtggaagata ttaaaaagat cttcagcaag
     5521 ccctggtttt tgaaggacgc ggactattac tgtaccatga gtatgggtat cgtcactttc
     5581 ccggatgccg gggattccgt ggctgatctg atcaaaaaag cggatattgc catgtacgaa
     5641 gcaaaaaaga ccggtaagaa ccgggtggca acctactgcg agggcattga ttccgtatcg
     5701 ggacgccgcc tggatatgga aaagaacatg cgtgatgcca cggtggaggg ctaccgggag
     5761 ttcgaggtct attatcagcc catcattgat attcagggcg gcagggatgt ctgcgccgga
     5821 gctgaggcac tgatccgctg gaacagtgca aagctgggat ttatctcccc ggcggaattc
     5881 atcccgttgg cagagtatct gggactaatc aatcccatcg gcaattatgt gctgaccgag
     5941 gcctgcaccc gctgcaaacg ctggaacgaa tccggttatc cgaattataa agtgaacgta
     6001 aatctttccg tagtacagct cctacagccg gacatcgtgg agacggtgga aaaggcgctt
     6061 gcggacaccg gtctgtcccc gaagaatctg accctggaag tgacagagag cctcgccatc
     6121 aacgatatgg agcgcatgaa ggagatcctg aaccggatca aatctctggg tgtttcgctg
     6181 gcgctggatg atttcggtac cggctactcc tccctgaatc atatccggga gattcccatt
     6241 gatgtcatca aggtggatca gagctttgtc aaagatctgg cggaggatgc ctactcccag
     6301 tcctttatca aaatggtggc ggagctggcg gagaccatcg gcgtcagcgt ctgcgtggaa
     6361 ggtatcgaga ccccggagca gttcagagta ttgcagggta tgaaggtcaa atacattcag
     6421 ggctattatt ttgaccgtcc tttggagagg catgcatttg aggcaaaata tgtgaagtaa
     6481 cagttcagcc atagaatagt aaaagcgatg atcgggtgct tgcacacgga gcatcgcttt
     6541 tgctattcta tgagcggagc gcccaaaaca tgaataaaag atgagctgca aagcagcgac
     6601 ggctgcgcag cagacgcttt tatgaatgtt ttggggcaga actgttacgg aagcaacaaa
     6661 attatcaaaa tccctcttgt caagtatcgg ggaaccatgc tactattaaa acagctacac
     6721 gactggcgga gcgcagctta ctgcagtgga gatacccaca gggagtgtag aatagtgaaa
     6781 gccgaccgcc tgggcaccgt ctatttctgg tgcttaggcg gttttttgta actattcaga
     6841 gccataaaat attgacaggt gttcgtcgaa tgttggcatt cgtaagaaca ttcggcaata
     6901 ttttatggtt ctgaatagtt acgcgatata tagtaaacgg aggaaaaact atgttacaac
     6961 tggaacagga attgaaaagc aatgcttatc ccggcagagg tatcgtgatc ggacgttctg
     7021 ctaacggtaa atatgccgtg accgcttatt tcatcatggg aagaagcgcc aacagccgca
     7081 atcgtgtgtt cgtgacagaa ggagagggaa tccgtaccca ggctttcgat ccttccaaac
     7141 tggaagatcc cagtctgatc atctacgcac ccgtacgtgt ccttggtaac aagaccatcg
     7201 tgaccaacgg tgaccagaca gatactatct acgagggaat ggacaaacag ctgacctttg
     7261 agcagtccct gcgcagcaga gaattcgagc cggacggccc caactataca ccgagaattt
     7321 ccggtatcat gcacatcgag aacggcaagt acaattacgc tatgtccatt ctgaagagtg
     7381 acaacggcaa tcctgattcc tgtctgcgtt tcacttatgc ttatgagaat cctcttcccg
     7441 gagagggacg tttcattcac acttatatgc acgacggcaa tccgctgccc agcttccagg
     7501 gcgagccgaa actggtagaa atccccgatg atatggatgc ttttaccgat atgctgtgga
     7561 acagcctgaa tgaggataac aaggtctctc tgtttgtacg tttcattgat atcgagaccg
     7621 gtaaatatga gacaaagatc gtgaataaga atatgtaaag taggttgtga agacgcgttt
     7681 tgcgaatgca ggttctgaat agttaatatc gtagaatagg agaaaaataa gatgaacgag
     7741 attcagttaa aatacggatg taacccgaac cagaagcctt ccagaatctt catggaagac
     7801 ggcagtgatc tgccggtgac cgtgctgaac ggcaagcccg gatatatcaa tttcctggat
     7861 gctttcaacg gctggcagct ggtaaaagag ctgaaggaag ctacaggact tcccgcagcc
     7921 acttctttca aacatgtgtc tccggccggt gctgcggtag gacttcctct gagtgatact
     7981 ctggcgaaga tctactgggt ggatgatctg ggagagcttt ctcccctggc atgtgcttat
     8041 gcgagagcac gtggtgcaga cagaatgtcc tccttcggtg acttcattgc attgtctgat
     8101 atctgcgata ccgataccgc ccgcctgatc aagagagagg tatccgacgg tgtcatcgct
     8161 cccggctaca ccgacgaagc actggaactg ctgaagcaga agaaaaaagg cgcctacaac
     8221 atcatccaga tcgatccttc ctatcagcct gctcctatcg agcgcaagca ggtatacggc
     8281 atcaccttcg agcaggggcg taacgaactg gatatcaacg gcgatctgct gtccaatatc
     8341 gtgactgtga acaaggagat cccggagagt gccctgatcg atatgaagat cgctctgatc
     8401 accttaaaat atacccagtc caattccgta tgttatgtca aggatggtca ggctatcggt
     8461 atcggcgcag gacagcagtc tcgtatccac tgcaccagac tggcaggcag caaggcagac
     8521 aactggttct tacgtcagtc tccccaggta ctgggcctgc agtttgtaga cagcctgggc
     8581 agagccaacc gtgacaatgc cattgatgtc tacatgggcg atgagtacat ggacgtgctg
     8641 gcggacggta cctgggaagg tatcttcaag gtaaagcccc ctgtatttac cagagaagaa
     8701 aagagagcat ggttggatca gatgcaggat gtgactctgg gaagcgatgc attcttcccc
     8761 ttctccgata acatcgagag agcacataag agcggcgtaa aatacatcgc acagccaggt
     8821 ggatccgtac gtgacagcga tgttattgca tgctgcgaca aatatgatat ggtgatggca
     8881 tttacgggca tccgtctgtt ccaccactaa gacagatttt gcgggcagga ctgtaacagg
     8941 acctgcctgt aactattcag agccataaaa ggcgattttg cgaatgtggt tctgaatagt
     9001 tttttgagga agaagaggaa agcgtagtta tggacagacg aggggatgca aagagcagag
     9061 aaatgatgct ggatgaatta ttgggctatg accatgtgac gatccagtgc catgacaatc
     9121 cggatgcgga tgccatagcc tccggctatg ggctgtactg ttttttccgg gacaagggaa
     9181 aggataccag gctgctgtat tccggacgga ataaagtcag aaaggccaac ctgatgctga
     9241 tggtggagaa actgcacatt ccgctggagt accagcctca gatggaagat acagtagacg
     9301 gactgctgat cactgtggat tgtcagtatg gcgcgggtaa tgtgacggag ctgccggcgg
     9361 aggagatcgc tgtcatcgac catcatccac tagaagtcat ctgcaccgag cggatgcgtc
     9421 tgcagcccaa tatgggaagc tgtgcaaccc tggtgtggac aatgctgcag gaaatgcatt
     9481 atcccgtgga aaaaaacaga gatctgggaa ctgcgctgta ttacggcctg tatatggata
     9541 ccaaccagtt ctccgaactg tcgaacccgg tggacatgga tatgcgtgag agtctgaatt
     9601 ttgataagaa ccagatatct ctgttccgta attccaatat ttccctgaga gagctggaaa
     9661 ttgccggtgt ggcaatgctg cgctgcaatt ataacgacga ttaccagttc gccgtgatcc
     9721 attcccagcc ctgtgatccc aatgtacttg gcctgatcag cgactttctc ctgcaggtgg
     9781 cgggcgtcaa tacctgcgtg gtctataatg aggacagcgg cggatacaaa ttttccgtca
     9841 gaagctgtat ccgggaggtc aatgccagcg aactgtcgga ttatctttcg gagggcatcg
     9901 gttccggcgg cggacattac gagaaggctg gcggctacat cagtatgaaa ttatatgagg
     9961 aaaaatatcc aacactgcat tccgaagcgt atttcaataa ccgcatgacg cagtatttcg
    10021 atacgttccg gatcctctac gccaaagacc gggcattccc ggtaagtgag ggcacaagat
    10081 atcagagaaa gaaaatccct ctggcatgtg tgagggcctc tgatctggcg gagcttggca
    10141 gggtggtgtc cgtccgcacc cagaacggca ccatggacat cgataccaga cagaatatct
    10201 gctacacgct agagagaaac ggtgaacttc acagggtggc gggagagcgg ttccacagga
    10261 tcctggaact gtccgacgct ccggtgtccg aggactactg caggaatatg acatacgttc
    10321 ccagggtcaa ggacggtacg gacggcagaa atcatctgat cacggaatat gtgcgcatgt
    10381 gcatgccgaa ggatgagttc cgcatctatg ccatccgggt gacacagggg gtaaaagtgt
    10441 tcccggtatg ggacgaggac ggctatatga ccggaagggc cggagattat ctggcggcca
    10501 gtgaggatga tctgcacaat atcttcatcg agacggagca gaatttgctg aattattttg
    10561 aagagaaggg tttataaaag ttttcttgtg tggtaagata aaggatacga aaacagtatt
    10621 aagtaaacga aaggcaagga cattaccatg agcgagatcg aaaaggaaat cgtagattcc
    10681 gcagaagtaa aggaatcgaa gaattttata gagcagatca ttgagaaaga tctggcggag
    10741 ggcgtatacg atactgtaca cacccgtttc cctccggaac ccaacggata tcttcatatc
    10801 ggacatgcca agagtattct gctgaactac ggcctgtccc agaaatacaa cggcaaattc
    10861 aatctgcgtt ttgacgatac caaccccacc aaggagaagg cagagttcgt agagtctatc
    10921 aaggctgatg taaagtggct gggagcagac tgggaggaca gactcttttt cgcatccaac
    10981 tattttgacc aaatgtatga agcagccgta aagctgatca aaaaaggcaa ggcatatgtc
    11041 tctgacttaa gtgcggagca gatccgtgaa tacagaggaa gcctgaccga gcccggcaag
    11101 gaagatcccg gcagcaccag aagcgtagaa gagaatctga cactgttcga ggatatgaag
    11161 gccggcaaat acgaggacgg cactaaggta ctgcgtgccc gcatcgatat ggcctctccc
    11221 aacatcaaca tgagagatcc cgtcatctat cgtgtggcac atatgtctca ccagaatacc
    11281 ggtgacaaat ggtgcattta ccctatgtac gatttcgcgc atcccatcga ggatgctatt
    11341 gagggcgtga cccattccat ctgtacgctg gaatttgagg atcacagacc cttatatgac
    11401 tgggtggtaa gagaactgga atatcctcat cctcccaagc agatcgaatt cgcaaagctg
    11461 tacctgacca atgtggtgac cggtaagcgt tatatcaaga agctggtaga acagggcatc
    11521 gtggacggct gggatgatcc cagactggta tccatcgcag cactgcgcag aagaggattc
    11581 acaccggaat ccatcaagaa gttcgtagaa ttgtgcggta tctccaaggc acagtcttcc
    11641 gcagattatg caatgctgga gtattgtatc cgtgaggatc tgaaggcaaa ggcaccccgt
    11701 atgatggcta ttctggatcc tgtgaaactg gtaattgata actatcccga aggacagacg
    11761 gagatgctgc ctgtagtcaa caatccggaa aacgaagctc tgggcagccg cgaggttccc
    11821 ttcggcaaag aactgtatat tgagagagag gacttcatgg aagagcctcc caagaaatat
    11881 ttccgtatgt tccccggcaa cgaagtgcgc ctgatgagtg cttatttcgt aaaatgcacc
    11941 ggctgtgtca aggacgagaa cggaaaagtc gtggaagtac attgtaccta tgatccggag
    12001 agccgcggag gcaacagccc cgatggacgt aaagtaaagg gaacgatcca ctgggtaaat
    12061 gcagagcagt ctgtaaaagc acaggtgcgc ctgtatgaga atatcatcga tgaagagaag
    12121 ggtgtataca atgaagacgg aagtctgaat ctgaacccca attctctgac cacactgaca
    12181 gagtgccgtc tggagcctgc atttgcacag gcacaggcgt atgaccgctt ccagttcgta
    12241 agacagggct tcttctgcgt ggactacaag gataccaagc cgggagaact ggtatttaac
    12301 cgtatcgtat ctctgaagag ttcttatgta ctgcctaagt aagcagtagg acaaccgata
    12361 aggggaattg ggaaagttgt attcgtattg tcaccgggaa tggacagtac aaaagaaaag
    12421 gagagacata tgtcggacaa attgtttgga ctggccggtc tgggtctgga tgacctggag
    12481 aacatgaaca tttacggaca ggataaaaaa gaagagaagg cagcagccga ggcccaaaaa
    12541 atagaagaaa aggatctgat ctttgacaag acatttacct gtcctgtctg cggagaagat
    12601 ttcccggcga agatcatgaa gaccggaaag gcaagacttt taagtaccga tcaggatctt
    12661 cgtgcaaaat acgaaggcat tgatgcggtg aaatatgatg ttattctgtg tccgcattgc
    12721 ggctatgcag cactgaaccg gtatttcaac agcctgaata aggtttatat aaagctgatc
    12781 aaggaaaata tcagcagtaa agtgcagctg catacctacg atgacgatat ttacagctat
    12841 gaagaagcca ttgagcgcta taagctctgc ctggccaacg ctgtggtaaa acgggcccat
    12901 gccagtgaaa aggcttatat ctgtctgaag agcggatggc tgatgcgtgg ttatcaggag
    12961 catctggaag agtccgggga tacggatatg gccagactcc gggaagtgaa gaccatggag
    13021 gagacttatc tgaagaacgc ttacactggt tttacggagg ccctgcagac cgaaggattc
    13081 cccatgtgcg gtatggatga gatcacggta gaatttctga ttgcggtact tgctgccaga
    13141 ttccagaaat atgatgtggc cagccgtatg gtagccacga tcctgacgtc cccttccgcc
    13201 aatgcccgga ctaaggacaa ggcaagagag ttaaaagacc agattctgga agaactgaag
    13261 aaccagaaaa agtaggataa ccgtatgtat gatatgacga tccggctgcg tacagacagt
    13321 gacaaatgtc tgtaccagca gatatatgag catatacgac aggaaatcag ggaagggaag
    13381 cttctggcag gagagaggct tccctccacg cgttctctgg cagaatatct gcaggtggcg
    13441 agaagcactg tggactatgc ctacgaccag ctgttaagcg agggatatat cgaggcacga
    13501 ccttataagg gctattttgt ctgccgtctg gaagggatat ttgccatgga accgcgggaa
    13561 gagaacgaac cggagccgga cacttcagga gaagaatctg ttccggaaga gggatgcatt
    13621 gatttttctc cttatggtat tgatatgaac ggtttccctg tgggcgtatg gaagcggatc
    13681 acgaagaata ttctgaatga caacaacagt gacctgtttg cgcagggaga acctcaggga
    13741 gattatgatc tgcggctgcc catcagccgg tatctgcctt cttcccgggg ggtgatctgc
    13801 cgcccggaac agatcattgt tggagcagga aacgattatc tgctgttg
//
LOCUS       DAWDHD010000062        13490 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_302_length_13490_cov_9.038630, whole genome shotgun sequence.
ACCESSION   DAWDHD010000062 DAWDHD010000000
VERSION     DAWDHD010000062.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 13490)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13490)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13490
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_302_length_13490_cov_9.038630"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3690 a         2928 c         3600 g         3272 t
ORIGIN      
        1 ggtgccggat ttcctacgca tgtcaagctg tctcccaagg atcctgacaa aatttaatat
       61 gtaattgcta actgtgcgga atgtgagcct tatctgactt ccgattaccg cagaatgatg
      121 gaagagccgg acaagctgat cggtggtctg aagatcatgc tgaagctgtt tgacaatgcc
      181 cacggtattc tggccgtgga ggacaataag ccggattgta tctctttgtt aaaacagatg
      241 actaagaacg atcctcagat cacggtgaag gcactgaaga ccaagtatcc tcagggcgcg
      301 gagcgtcagc tgatctatgc cacaacggga cggaagatca attcctccat gctgcctgcg
      361 gacgtaggtt gtattgtaga taacgtagat actgtggttg ccatttacag agcggtgacg
      421 gaaggacgcc ctctgatgga acgtatcatc actgttaccg gagatgctgt ggccaatccc
      481 cggaattttc gtgtacctat cggtatgagt tatcaggaac tgttggatat agccggtgga
      541 tttaagcagg agccggagaa gatcatctgc ggaggtccca tgatgggatt cggtatgttc
      601 cagctggatg tacccaccac aaagacctct accgcattac tggcactgac ccaggacgat
      661 gtatccgcta tggagcccag tccctgcatc aactgcggac gttgcgtgga agcatgtccc
      721 ggccgtattg ttcccagcct tttggcaacc tatgcggagc attttgacga agaggctttc
      781 gtggaacata acggtatgga atgctgtgaa tgcggctgct gtagttttgt atgcccggca
      841 aagcgtcctc tgacgcagga gatcaaatcc atgcgtaaga tccagcttgc caagaagaaa
      901 aagaagtagg agggaagaac agtgagcgga cttttaaaag tatcttcaaa tcctcatatc
      961 cggtcaaaag taacgaccag cagcattatg acagccgtgg tgatcgcact gctgccggct
     1021 gccggtttcg gaatctataa ctttggcccc agagcactgg cagtcatggc tgtgaccatt
     1081 gccagtacag tattgacgga gttattattc ggctggctct ggaagaaaaa aatcacgatt
     1141 tccgatatgt cagcagtggt aacgggactg ctgctggcgc tgaatctgcc ggtgagcatt
     1201 ccgctgtgga tggcggcatt gggcggtgtg tttgccatcc ttgtggtaaa aatgctgttc
     1261 ggtggtctgg gtcagaactt tatgaacccg gcactggcag ccagatgctt tctgctgatc
     1321 tctttcccgt cccagatgac gaattttgcc tgtgatgcct atactggggc tacaccgctg
     1381 gcagcactga aggcaggaga gagtgtcaat gtcatgaaca tgatcgtagg taagaccgcc
     1441 ggtaccatcg gcgagacttc cgtgatcgca ctgctgatcg gtgcctgttt cctgatcctg
     1501 atcggtgtga tcgaccttcg gatccccggt acatatattg tgaccgtgat cctgtttgcg
     1561 ggaatcttcg gcgggcacgg ctttgatcct gcctatctgt ctgcacagtt agcgggcggc
     1621 ggcctgatcc tgggtgcctt tttcatggca acggattatg tcaccagacc gattaccatc
     1681 aagggacagt atgtatacgg tgcggtactg ggacttctta ccggaatctt ccgtatcttc
     1741 ggaccgggag cagagggagt gtcctatgcc atcattttag gtaaccttct ggtacctctg
     1801 attgaaaaat tcaccatgcc ggtggctttt ggcagaaagg caggtgcgaa acgtgaaaaa
     1861 taagaatgat attcaggcaa tgttaaaaga agcgggcatt ttgtttgcaa ttactctgat
     1921 tgccggactt ttgcttggct ttgtctatga actgaccaag gagcccatcc gtcagcagga
     1981 ggaaaaagcg gtgcaggaag cctgtcaggc ggtctttgcg gatgcggagc attttgaaga
     2041 actggatgct tatacacctt ctgcggatac ggcgcagaag ctggcagata ccggaatcac
     2101 catcggtacc gtctatgaag cacaggatgc ctccggaacg cagttgggct atgtgatcca
     2161 gaccacgacc tccgaaggct atggcggaaa tattgtgctg tatgtgggaa tccgtctgga
     2221 tggtacggtc aatgatattt ctattttaag tatttccgag actcccggac tgggcatgaa
     2281 agcgggagat gtgctggtgc cgcagttcca caacaagaac gtgaagagct ttgcctacac
     2341 caagaccgga tccacttctg acagtgagat cgatgccatc agcggtgcga ccatcaccac
     2401 gaaagcagtg accaacgctg tcaacggaag ccttctggca tttaatgatt atgtacaggg
     2461 aggtgctggc aatgaataaa gcaggtgaaa gactttataa cggtatcatc aaggaaaatc
     2521 ctacatttgt actgatgctg ggaatgtgtc cgactctggc ggtgaccaca tcggccatga
     2581 acggactggg catgggcctg accacggcag tggtacttac catgagtaac ctgatcattt
     2641 cgctgcttag gaaagtgatc cccagccggg tgagaatccc tgcatttatc gtgatcattg
     2701 catccttcgt aactgcggta cagttgctgt tgcaggcgta tctgccgtca ctgaacgatg
     2761 ctctgggtgt gtatattcct ctgatcgttg taaactgtat cattctggga agagcagaga
     2821 gctatgcgta ttcccatccg cccattcctt ccctgttcga tggacttggc atgggacttg
     2881 gtttctctct ggcactgact tgtatcggtg cagtacgtga gatcctggcg gccggatcca
     2941 tcttcggatt tcggatcatg ccggattcct atgtgacgat caatattttc ggactggctc
     3001 ccggtgcgtt cttcgtactg gctgcactga ccgctttgca gaactatatc aagaataaga
     3061 gaaagctggc tggcaaggat tacgagaaga tccagtccgg ctgcggacat gactgtctgc
     3121 actgtggcga gagcggctgc tcggagcggt tttatgacaa taccaatacc acggaagagg
     3181 ctgtgaagga tgcggcaaaa gcggcagcaa aacctcagac ggcagaggat gatctggaga
     3241 ccatcgattt agataaggag gacaaataaa tggatagtgt aaaagatatg cttctgttac
     3301 tgatcagttc ctccatcgtc agcaatatcg tattaagcca gttcatggga ctgtgcccct
     3361 tcttaggcgt gtcccgcaag atcaagacgg cgggcggcat gggaacggcg gttatcttcg
     3421 ttattacgct ggccagtgcg gtagcgggtg tgatctacaa atttatctta cagccgttac
     3481 acattgagta tctggagacg atagtgttca ttctcgtcat tgctgcactg gtacagttcg
     3541 tggaaatgtt cctgaaaaag gcaatgcctt ccctgtatca ggcactgggc gtctatctgc
     3601 ctctgattac caccaactgc gcggtgctgg gtgtagccat taccaacgtg cagaaggaat
     3661 acggtattct gaccggtatc gtaaatggct ttgccacagc gtttggcttt accattgcca
     3721 tcgtgatcct ggcaggactg cgtgagaaga tggaatacaa tgaagtgccg gaatctttca
     3781 agggaatgcc catggtactg ctgacagcag gactgatggc cattgctttc tgcggattct
     3841 ccggtctgat ctaggaagga gtaacataga tgagtataac aggaattctg attgctgcgg
     3901 cttgtgtcgg aatcgtcggc atctttgtgg gactgttcct tggcgtggcc ggcattaaat
     3961 tcaaagtaga agtagatgag aaggaagagg cggtgttagc tgcccttccc ggaaataact
     4021 gtggtggctg cggcttcgcc ggatgttccg gtctggcagc tgccattgca aagggagagg
     4081 ctgcggtgaa cacctgtccc gtaggcggag aggaagtcgg caaaaaaatc gccgccatca
     4141 tgggtgtaga agcagaggct tccgagcgca aggtagccta tgtgcattgt cagggtgact
     4201 gcgagcggac cacaacggat tatgattatt atggcatcaa ggactgtcgt atgatgagct
     4261 ttgtgcccgg aggcggaccg aaatcctgca acagtggctg cctgggattc ggaacctgtg
     4321 aacaggcctg tccctttgat gccattcatg tagagaacgg tgtggctgta gtagataagg
     4381 aaaaatgtaa ggcctgcgga aagtgcgtgg aggtctgccc caagcatctg atcagcttac
     4441 ggccttattc caatacggta caggtagcct gcagttctac ggacaagggc cccgttacca
     4501 tgaaggcctg caagaccggc tgtgtgggct gcggtatctg cgtaaagaat tgccagcacg
     4561 atgctgtgaa ggtcgagaat ttccatgcaa tcattgatcc ggagaaatgt gtgggctgtg
     4621 gtgcctgcat ggagaaatgc ccgaagaaat gtatcgtaaa agcataaaga aagtacaaga
     4681 ggagaaaaga tatgcgtctt ccggacgatt atgaagatca cggaggaggg ctgacaccta
     4741 cggtgatcag tgccatcgtt gctgttacaa ttttcgtggc agtgatcctt acggtggtgc
     4801 tgatcatgaa cagacagccc acttcccacc aacagaacaa tacggctgca cagacggaga
     4861 atgtacaaca ggcaggaaat aatcagacag acaaatatcc ggacacgcag gacctgatca
     4921 ccggaagcac attgactcct tcggatctgg atttctggga catgtacccg gagactacgg
     4981 caacaccatt gccgtctgcg gatccttccc aaaaggataa caaaaaggaa acagcggaag
     5041 cggatccctc caccgatgga aaacataccc tggtgaccaa ccgggacggc aaggaagaat
     5101 gggtgctgat cagcccgtac cttcccaagc acgaatatga tttcaccaaa ctggtgtgcc
     5161 agtccgacct gatgaaatat tatcaggacg gcaaactgac ttcctatgtc ggggtggata
     5221 tttccaaata tcaggattat gtggattttt tgaaactgaa aaaagccgga gtggattttg
     5281 tgatgttgcg ggtaggtgcc agaggctatg gcagcggaca gatcgtattg gatgattatt
     5341 tcgtagacaa tatcaagaga gccacagatg ctggattaca gatcggggtg tattttacct
     5401 cacaggccat cacggaagaa gaggcggtgg aggaatccaa tatcgttctg gagaatatca
     5461 aagactacaa gatcacctat ccggtggctt ttgatatgag ttttgtggac aatgatacga
     5521 cgagaatcga gactgtcagt cgtgcggaca agacgaagat caccaaggct ttcctggata
     5581 ccatagaggc agccggatat aagcccctgc tttacggaga caaggagtgg ctgatcaaag
     5641 agattgacct gtcaaaatta tcggcatatg acgtatggct ttcccagatg gaagacgtgc
     5701 cggattatcc ttatcgtttt accatgtggc aatatgccaa tgatgtcacc atcgacggca
     5761 ttgcaggcta tgccaatatg aatatcagtt ttattgatta ttcggagaaa taggctcata
     5821 attcaggatt cccggtgcaa tgtcgagcgt agaatagatc tcggcataac ggcggggagt
     5881 gatggcttcc acatagttgg gggtaaaatt actggtgact cctttgctat gcaaaatatc
     5941 gatggccttg ttataggcaa tggctgcttc gatatcagta gcgtagcgcc ccacaatata
     6001 attaccacgg atatgaatgc ggacagtata aacgtactgt ccgttttttg ctgccgtttt
     6061 ccggacaccg tgatagatgt tgtggatctg cagattttcc ctgcggaagt ccgtaggatc
     6121 tccgttgaca aaacaataat ccacgccggg aactccgtag cttttgatgc catagcgtga
     6181 ggtcagcgtc tgctgcattc cgtagtcggc cacaaaataa tgatttcccc ggcacatgat
     6241 cttatgggaa gaataataga acaggtcttc catgtcgaat tttaagacat ggtgggggga
     6301 caaataataa tagaagagct gctgccccag atagatcgga ttgccgatgt atagtccgtt
     6361 gtcccgcaga ttgatcagac tgacccattt ttcgaaggac aaaggggaca ccttctcgta
     6421 ggactgcagt gtcagggaag gatcggacag caggagaagc ccctgttcgt aggcccgatg
     6481 ggcctgtaag gcatcggaga aactgccaag actgatatgc ttgccgtttt tggtcagaga
     6541 agcacggtaa tagacagttc cgtctttttt tccggcccgg aagactcctt tatataattg
     6601 ttctttttca atgttttgct cactcatatt tctatagtaa catttttctc cggagaacaa
     6661 cagatatatt tttcggacga gctgcataaa atgaagttag agccaaaatg agaattgggg
     6721 aaaggaacaa agcctgtatg tataaaagag cattaagcgg actacttgca ataaatctga
     6781 tatttctgat cggtgtttta agtctctgcc gcatggaaga aatctgtagg aaattgcagc
     6841 agacggtgtc ggaggtacat atggaagaaa gcctgctggc ggacaaggaa agcatcatga
     6901 tggggattgc ttcccggact tcttccggac agagagtagt ggattgcagt gaattacagc
     6961 gggagagtaa gtaccagctg gaacaggcgg atctggaagt gctgcttcgg atcgtggaag
     7021 cggaagccgg ttgcgaagac gaggatggga agctgttggt ggccaatgtg atcctgaacc
     7081 gtctgaattc cgaaaaattt ccggacagca ttacggagat cgtattccag cgggaaaacg
     7141 gggtgtcaca gttttctccc attgccgacg gcagttatta caaagtaagt gtcagtgaag
     7201 agacggtaga agctgtcgga cgggctctgg cgggcgagga catttccagg ggagcattgt
     7261 attttgccgc cagaaaatat gccgacagtg aaagtatgcg ctggttcgat gaaaaattga
     7321 cgctattatt ccgacatggc ggacatgaat ttttttcgga taaaaaagcg cttcaatgat
     7381 tcaccttgca acagtgttta aagtatgcta tacttttcga aaatatagat ggagatgcta
     7441 gggagatatt ccagcataca ttcatacata gacacggagg agaggaaatg gaagtaattt
     7501 atcgcagcac acgcagcaac agcgaaccgg taaccgcatc ccaggcaatt ctgaagggat
     7561 tatccgatga cggaggactg tttgtaccgg atcatattcc tgcattggac aagagtcttt
     7621 cagaactggc tggaatgagt tatcagcagg tagcttatga agttatgaaa ttattcctga
     7681 cagactttac tgaggaagaa ctgaaaaatt gtatcaacaa cgcttatgat tccaagtttg
     7741 atacggagaa gatcgctcct ctggtacagg cggatgatgc ttattatctg gaattgttcc
     7801 atggttccac cattgcgttc aaggatatgg cactttccat tttacctcac ctgatgatca
     7861 cttccgcccg taagaataat atcaagaatg agatcgtgat cctgactgct acttccggtg
     7921 ataccggtaa ggcagctctg gcaggctttg ccgatgtcaa gggtacccgg atcatcgtat
     7981 tttacccgaa gaacggtgtc agcccgattc aggagaagca gatggtgacc cagaagggtg
     8041 ccaatacttt tgtggtaggc attcacggta atttcgatga tgcccagacc ggtgtgaaga
     8101 agattttctc cgacaaggaa ctggcaaaag aaatggatga gaagggcttc cagttttcct
     8161 ctgcgaactc cattaacatc ggacgtctgg tacctcagat ttgttattat gtttatgcgt
     8221 atgcacaatt attaaaagat ggtaagatca cagagggtga gaagatcaat gttgtggttc
     8281 ccaccggcaa cttcggtaat attctggctg cattttatgc gaagaatatg ggactgccca
     8341 tcgataagct gatctgtgct tccaatgaca ataaggtatt gtatgatttc ttccgcaccg
     8401 gcacctatga ccgcaacaga gaattcgtac tgaccacttc accgtccatg gatattctga
     8461 tctccagcaa tctggagcgt ctgatctacc gtattgcagg caatgatgca gacaagaacc
     8521 gtgagctgat gaacgctctg tccggcaacg gcaaatacga gatcaccgaa gagatgaagg
     8581 cacagctggc tgacttctac ggcaactttg caagcgaagc ggagaccgcc gcagagatcc
     8641 gccgtctgta tgaaaaatgc ggttatgtca tcgataccca taccgcagta gcttccgcag
     8701 tgtacggcaa gtatgtggtt gagaccggag atcacaagac caccgtgatc gcttccaccg
     8761 caagcccctt caagttcacc agaagcgtta tggatgccat tgacatcaag tatgatgcca
     8821 tgggtgactt tgaactggtg gatgaactgt ctaagattgc caaggtgaaa gtacccaatg
     8881 ccatcgaaga aatccgtacc gcaccggtgc tgcacgatac ccagtgtgac gtggacaaga
     8941 tgaaagacac ggtgaagagt tttcttggaa tgtagttaag aattaaaaat tagtgagata
     9001 ccgcccaagt cacgtcaacg gctttctggg cggtattgtt tttgtgtaaa aactttatcc
     9061 tttatcttgc gggggttctt tgctataata ggtaaggtta gcagactgta acaataatat
     9121 gaaaatagat accggaagga gaacaacatg actgcaaaag aaatgcttgg aaaagtggat
     9181 catacccagt tgaaagcctt tgctacctgg gaggacatcc agaaattatg cgatgaggcg
     9241 attcagtatc ataccgccag cgtatgtgtg cccccttgct atatcaagag aatccatgac
     9301 acttacggag acaaaatcaa tatctgtacc gtagtaggat tccccttagg atacagtgtg
     9361 acggaagcca aggtggccga ggtgcgcaag gcactggagg atggttgcaa cgagatcgat
     9421 atggtagtga atatcagcga tgtgaagaac gggttgcttg acaaggtgga ggaagaaatc
     9481 cgtacactga agaaagagtg cggacatcat atcctcaagg tgatcattga gacctgttat
     9541 ctgaccgagg aagagaagat tgccatgtgt aaggcagtga ccaatgccgg agcggacttt
     9601 atcaagactt ccaccggctt cggaaccggc ggcgccacaa aggaggatgt ggaactgttc
     9661 aagaagtaca ttggccctaa cgtaaagatc aaagcggcag gcggcgtatc cagcatggaa
     9721 gatctggaaa tgttcttagg acttggctgt gaaagaatcg gtacttccag agctgtagga
     9781 ctctgcaagg gtgaacttac ggaagggtat tgattaactg atatatagat gatatataga
     9841 atggagataa catggaaaac aaagtgattc aggacagact tgcacttctt cggaagaaga
     9901 tgcaggaaga gggcattgat ttctatatga tgcccacagc ggattttcat aattccgaat
     9961 atgtaaacga ttattttaaa gtaagagaat atttctgcaa ttttaccggt tccaacggca
    10021 cactggtagt ctggaaagac ggcgcgggac tgtggactga cggcagatat ttcatccagg
    10081 cagaagcaga attggaagga acgaccgtag aactgttccg tatgttacag gagggagttc
    10141 ccaccatcga ggaattcctg gagcagaaca tgcagcaggg acagacttta ggatttgatg
    10201 gcagagttat cgctgccatg gacggcaaaa aatacgaaaa ggtactggaa aagaagcaga
    10261 tccgcatcgc ttatgagaag gatctggcag agagcatctg gacagacaga ccggattttc
    10321 ctgccggaaa agtaaccgta ctggatgaga aaatcgccgg taagagcgta gaggaaaaac
    10381 tgacagagac cagagaaaag atggcgaagg acggtgccaa tgcactgctt ctgaccaaac
    10441 tggatgatct gatgtggctc ttcaatatcc gcggttgtga tgtggaatgc aatccggtag
    10501 caatgtccta tgcctatatt acagaagagt ccgcaactct gtttatccag cagaaagcac
    10561 tggatgcaca ggtgacggaa tacctggcag ctcatcgtat cgaggtaaag gaatacgaca
    10621 ccgtcgtaga tttcctgaag gaactccctg cgggcaatgc gatcctcgtg gacaacagat
    10681 attgcagcta tactttatac aagacattgc agaagaatca gaagttagta gagggcaaga
    10741 accccaccga actgttaaag gccattaaga atccggtaga gcttgcgaga atgcaggaga
    10801 tctttttaaa ggacagcgtg gccgtaacga aatttatcta ctggctgaag acccatgtgg
    10861 gcaaagagaa gatcactgag gtgaccgcag cggactatct ggaacagaag agaagagaga
    10921 tcccggaatt cttagatctg tccttcccga ccatcgcagg ttacaagtcc aatgcagcca
    10981 tgatgcatta cgaagcaacc ccggacaact gtgcgactct ggagccggaa ggaatgcttc
    11041 tggtagattc cggcggacag tatctgggag gtaccaccga tgtgaccaga accatcgttt
    11101 taggtcccat ttccgaagag atcaaaaagc attacaccat ggtagcagcg gcagtgatgc
    11161 agctgaccca tgcacactgg ctctacggct gtaccggacg gaatctggac attctggcaa
    11221 gacagcctat ctgggatatg gacattgatt accagtgcgg taccggacac ggcgtaggtt
    11281 atattctgaa tgtgcacgaa ggtccccaga acatgcgctg gagattcacc ggaggtatgg
    11341 tggaggcagt gtttgaggat ggtatggata tcaccaatga gcccggcatc tatattcagg
    11401 gcagccacgg tatccgcatc gagaatgtta tggtggcgaa aaatgatgtg aagaacgagt
    11461 atggtcagtt catgcatttc gagaccctga catgggtacc catcgacaga gaagccattg
    11521 atgaaaagta tctgaacgat acccagaaaa agtatctgca cgagtatcag aagactgtct
    11581 acgagaagat ttctccctat ctgaatgagg aagaaaaaga gtggctggcc aaggaaacca
    11641 gggtaaagta aagagaatat aaaatcagta taaaattttt agcggtttct tgactttttt
    11701 actggggttt gagataatac agacattgtg gcagaagata aagacacaca taaatttaaa
    11761 ggaaagaggt taatgtaaga tggcagagat caatttaagc aagtacggca tcaccggaac
    11821 cacagaaatc gttcacaatc cttcttatga agaattattc aaagaagaga tgaagcctga
    11881 actgaaaggc tacgagaaag gtcaggagac cgaactcggt gcggtaaatg ttatgaccgg
    11941 tatatataca ggacgttctc ccaaagataa gttcatcgtt gaagacgaaa actccaagaa
    12001 cactgtatgg tggacttccg atgagtacaa gaacgacaac catcgtatgt ctcaggaaac
    12061 atgggctgct gtaaaggata tcgctaagaa ggaactgtcc aacaagagac tgttcgttgt
    12121 agatgcattc tgcggtgcta ataaggacac ccgcatggct atccgtttca tcgttgaggt
    12181 agcttggcag gctcatttcg taaccaacat gttcatcaaa cccaccgagg aagagctgaa
    12241 gaacttcgag cccgatttcg tagtttacaa cgcatccaag gctaaggttg agaattacaa
    12301 ggagctgggt ctgaactccg agacttgtgt agcatttaat atcaccagcc gcgagcaggt
    12361 tattgttaac acatggtacg gcggagagat gaagaagggt atgttctcca tgatgaacta
    12421 ctacctgcct ctgaagggca ttgcttccat gcactgctcc gcaaacaccg atatgaacgg
    12481 tgagaacacc gcaatcttct tcggtctgtc cggaaccggt aagaccactc tgtctaccga
    12541 tcctaagcgt ctgctgatcg gtgatgacga gcacggctgg gatgacaacg gcgtattcaa
    12601 cttcgaaggt ggatgctacg ctaaggttat caacctggac aaggattccg agcccgacat
    12661 ctacaacgca atcaagagag atgctctgct tgagaacgta accgttgatg ctgacggcaa
    12721 gattgatttc aacgataaga gcgttaccga gaatactcgt gtatcttatc ctatcaacca
    12781 tattaagaat atcgtacgtc ctatttcttc cgcacctgca gctaagaacg taatcttcct
    12841 gtctgctgat gcattcggcg tactgcctcc cgtatctatt ctgactcccg agcagactca
    12901 gtactacttc ctgtccggtt tcacagctaa gttagctggt accgagcgtg gtattaccga
    12961 gcctacccct accttctctg cttgcttcgg ccaggcattc ctggagctgc atcctaccaa
    13021 gtatgcagaa gagctggtta agaagatgga gaagaacggt gctaaggcat atttggttaa
    13081 caccggatgg aacggaaccg gcaagcgtat ctccattaag gatacccgtg gtatcatcga
    13141 tgctattctg gatggcgcta tcaagaccgc tcctaccaag aagatccctt acttcgattt
    13201 cgaagttcct accgagctga ctggtgttga caccggtatt ctggatccca gagataccta
    13261 cgctgatcct tcccagtggg aagagaaggc aaaggatctg gctcagagat tcatcaagaa
    13321 cttcgctaaa tacgaaggaa atgatgctgg taaggctctg gttgctgctg gtcctcagct
    13381 gtaatcttac tttacagaca aatgcaaata tggaaggctg tctcttcgga gacagcctca
    13441 gactgtagac aaaacgcttt tggtcagcac tccagaagcg tttttcttgt
//
LOCUS       DAWDHD010000063        13196 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_307_length_13196_cov_7.801309, whole genome shotgun sequence.
ACCESSION   DAWDHD010000063 DAWDHD010000000
VERSION     DAWDHD010000063.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 13196)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13196)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13196
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_307_length_13196_cov_7.801309"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3295 a         3446 c         2764 g         3691 t
ORIGIN      
        1 tgtatacgag agactctata gtgtatgcgt cggaagccct gtcctgcctt ccttccctga
       61 gtgcggtctc ctcatgggtc tccccggctt cgacggtacc cttcggcaat acccagcctt
      121 catatttatt cttaatgttc ttatacagca gcaggatctt cccacggaag atcacaacgc
      181 caccgcaact ggtagcctct atcatgattc ttttgactcc tccgatcact ctttctggtt
      241 gaagtaagca tcaaacagac gtcttgcaat gggaactgcg taatcaccgc cacttcctgc
      301 accctctacg ataatggtga cacagacttc cgggtcttct gcgggtgcaa aaccggtaaa
      361 ccaggcatgg ctgtcaccct tgatattatt gtattccgca gaacctgtct taccggcagc
      421 cgtatagttt aaacctctta acttggaagc agttccctct tccacaacat cggtcatcaa
      481 ctgtttcaga atcgcagatt cgtcctctgt catcagacgg ccgtattccg atgccttaaa
      541 gctttttacc acagtacctt catcattttc cacatgatca atgacatagg gtttcatcag
      601 aacaccatcg ttggcaatgg cacaggtgat catattcaga tgcatcggtg tgatctgggt
      661 cttaccctga ccgatggagg tctgcatcat ttccgcggaa ggtgtacttt cggagacgat
      721 agccgagctt ttggcataat tcagcgtaag tggcagatct ttattgaaca aaaggtcatt
      781 cagtgtctcc tgaaaagcct ctctgtccag gctcattcca atattggcaa aggacgagtt
      841 acaggatttt gcaaaggact tctgaaagct gacctgtccg tgactggaac cgtgataaca
      901 actgattttg ctgtttccca cccggaaaga accattacag ttataagaat aattctgata
      961 cgtggtggga ttctcccgga tatattccaa tgccgttacg atcttgaagg tggatccggg
     1021 cggataaagt ccctgggtgg cgcggttcaa cagtactgta ctggaatcat tgtcgatcag
     1081 atcatcccag atctccggaa tggtattcgg atcaaaatcg ggatgcgaca ccattgccag
     1141 aatcttgccg gtagacactt ccgttacaat gatggcacca cggtaaatat ccagctggtc
     1201 gttggccacc tgctggatat ggacatccag tgtggtatat acattatctc cgggattctt
     1261 tttcccggca gtatcgtttt tcactttatt gctcagggag gtattggtgt tgatcagata
     1321 gtaattggcc agagcctcca ctcccatgcg tccctgggtg gaataaccta cgatatgaga
     1381 aaacagattc ttatacggat agtttcgagt ctcctcttca tccgcatcca gtatcgtctc
     1441 tgccagaaca tccccatccc tggaataaat ggatccgcgg taattgcggg acagcagaat
     1501 ctgctgacgg gaattataac tgttgttgat catatcctgt tcgctggttg ccacaaaatg
     1561 acccagatag accaacaggc ataagaacag gacaccgatg caaccagcag aaagccagat
     1621 ttcccgctgg ttgcccatac gttttctgtt ccgggcgtcc tttttgcggt cggcatcttt
     1681 tttctgtgta tttttctgcg tattctgcgc cttattttct gccacctctg cgctctcctt
     1741 ccttctgcag gcgaatatag attccctgta taatgaaaaa catgatcatg gtcgtcatga
     1801 cggaacttcc tccatagctg atgaaaggaa gtgtcactcc ggtcagcgga atgaacttgg
     1861 taccgccgcc aatggtcagg aatatctgga aaatatacat aataccgatg ccgtatacga
     1921 tcagctgata gaatctgtca tgaatgcata ctgccacccg catcatttcc agaaaactgc
     1981 tgacacagat caggatcaga catattccga agatcacgcc cagttcctcg cagatggaag
     2041 aaaagataaa gtccgcttcc acataaggaa tcgctgtggg attacctttt aacagtccca
     2101 tgccgaacca gcttccactg ccgatggcaa ataatgactg ggtgatctga tagcctttat
     2161 tgtcaatata tttccaggga tcctgccagg ccagtactct gacccgtaca tgatcaaaca
     2221 gctgataagc cagataagaa gccgcacctc cgccgacaag tcctgccaga agatacagat
     2281 aatttcttgt ggcagcaaaa accacaaaaa caaagcccac gaaaaagatc aaagcacttc
     2341 ccaggtcctt ggatactacc aggatcacca catgggctcc cgcaatgatc gccgtcagaa
     2401 cgactctctg gaaggagaca ttttcccaca acgctcccgc cagaaagaac aggaacagga
     2461 tcttgacgaa ttccgaaggc tggaacgtga ttcctcccag ggaaaatgaa atcttggaac
     2521 catgggtcac ctctcccagg atcagcaccg tactgagcag cgcaattccc aacgtggcat
     2581 acagccaggt gatcttctta aaaaagtgga tctttcccag cagatacgga accagcaggg
     2641 aaaagatcag agataccagt acgatcacat attgccggat ggccttgtca aaagataatc
     2701 tcgatacgat gcaaagaccg attcccagaa gcatacacat attattcagc aaaagcctgt
     2761 taatattctc gtagatcagc gataccatcg tgatcgtagc aaacagaaag atcagtataa
     2821 atgcaaagaa aaacaaatac tgaaaatctc cgctgacaag ggacaggttc agaaaacaga
     2881 ggacctgcac cagaaacatc aaaagaccct gtaaaataaa tactccacga tgcttctctc
     2941 ccaccggata ccgaaaggta taaaaacagc ttgccgtata taacaccata aaaaaggcga
     3001 taaaatattt ggataattca atcacatatt cctgcatgga tccaaaacct ttctgtctat
     3061 tctactccac gtttttcatg accggtggta tattccccat agggcaattc cttacgggta
     3121 ccggcaaaat actccaggac ttccaaagtc tcggtctcat tttccgtggt aaaatccagc
     3181 cgtccggtca ccaggccgct ccatttacat aattccttgt gcaaggaaag cggaacactg
     3241 ttatagatca cattaaaaca gtgggtacaa ttccgctgaa ccggaaatct tttatgatag
     3301 cggtcgatca gatccaatgc cttcggattc tcctgcggct gacaccggtc agtggtcttc
     3361 tgcacgcagt tggcggtgac catcataggg atcctgccgt atattacttt ttccgcagga
     3421 aacgcaggat ccagaatgtc cctctgctct gcggcattca gctccaacgg cagacaatag
     3481 ccgtctaaaa atgccttcca gaaatcccgg ctggtccggt tccagagata cagactatga
     3541 tccccataga tttcttttcc ggtaaaattc cattgtttta ataacgcgat atgttccatg
     3601 tttgctgcct gaaagccatt tatatatgcc agattttcca gcagcaattt ccgtaatttt
     3661 tcaagatact gcccctcccg caggcgcagg atcttcggca gagcgatcac agattggatc
     3721 tcttttttag taaggcttct caaaatcttc tccgcagcgg acggatcctg taacaggaaa
     3781 tccgcatcca gatagagtct gagttgtccg gcggttctga ccggatcttt ccagaaggat
     3841 gaactgcaaa agccctgcca ttgttccggg gtccgtatcg tgacagccca tttttcatcg
     3901 gatttttgag actttatact atcgtttttc cgaaaatcac catcgtcagc cagtgtcgga
     3961 gctttttcgg aagttctctc ataaaggaag ccgtttgcaa ggatcacttg ttctttcagc
     4021 agggaaagtc cctttcggcg taactcgttg atagctttta acgggtagaa agcattgtca
     4081 cttacccgga ttccgatgga gtcctccaga tggaagtgac tgtcgccaag tttccccagc
     4141 tgttttgcta tattttcctg tgtgatgggc tgtcgggaag cttcttctac cacattccct
     4201 tctacggtga cggcactctc tccggtccac aaatacaatt ttgcggtctc cccggtcaga
     4261 aattcagcct gcatggatac cggaagggtc agcctgtgtt ccagatgctt cttccggata
     4321 ttctgaagca aagtctcatc actaccgctg taagccgggc tgtccagagt caccatctcc
     4381 ccgccgttgt gcttgtgata ataaccatcc tgtctctcac tgcgaatata gaggcaggaa
     4441 agcgtatgca tatctgccgc agaaatcttc tgaggagctt tggaatccgc ataatactgg
     4501 tcaatatatt tgcgataaat tgccgtaact ccggcggcat attccggctt cttcatacgg
     4561 ccttcgatct taaaggagtc gatccctgcc tccagaagct ccggaatata ttcaatggta
     4621 cacatatctt tcaggcttaa aggataacat tccttcggat gttttccagt ggtatagggc
     4681 aggcgacaag gctgggcaca acgtcctctg ttgccacttc tgccccccag aatactgctg
     4741 aacaggcact gtccggaata gcaataacac atggctccat ggataaagca ttcgatctca
     4801 agccctgtga cttccttgat cctgcggatc tccgcaaggc tcagctctct ggcgggaact
     4861 acacgacagg ccccctgttc cttcagaaaa gctgccccat actcccctgt aatggtcatc
     4921 tgagtgcttc catggagttc catcccggga aaatgttctc tgagatatgc aaacactccc
     4981 atgtcctgga caatcacccc atccagtccg gcccggtaat agggcaatac atattcatac
     5041 agtccggaaa attccgattc ttttaccagt gtgtttaccg tcagatatac ttttcgctga
     5101 aaaagatgag cataccggat gcaggcaacc agttcttctt ccgaaaaatt ttccgcataa
     5161 gctctggctc cgaaacggct gccacccaga tatatagcat ccgcaccggc atggatagcc
     5221 ccgtaaaagg cttccgcatt tccggcaggt gccagtaatt ctactttttt tgctgctttc
     5281 atagatttca gatttcctct attgcattcg attcatataa gcaaatagct gcccgaaaac
     5341 ggacagctat caatttattt gtcttttaat tctgtctcca gacgtacaat cttcttggcg
     5401 tcttcattta actgcttttg cagttccttc ccctgctttt ctgcattttc cagtttgatc
     5461 tgtgctgaaa tcagttcatg cttcagatca taaagctctt tttccttgcc ctgcagttct
     5521 tcctccagaa tggagatctg cttcttggct ttaaaataat catctgcaat attcaactgg
     5581 agcagaatgt tctgcttatc cagaggttgt ctgcgaaagc cgtccagctt cccgtattcg
     5641 gtaatctttc cattgatgta ggaagcaatt ttctgcatat aatcttcact ttcatagccg
     5701 cttaaagtaa atacttttcc gcctatgatc acttctgtat ctgtcttagg tgacatctcc
     5761 acacccctct taacactctt ttgaaaaaca caatatatgc catttacgca aaaatataat
     5821 acaagattta ttatatatca gaatgtatag ggtggcaagt aaaatttcgt aagctgcacg
     5881 tatcaattca gtcccagatg atgataggta agatctgtga ctacacggcc tttgggtgtt
     5941 ctgttcagaa atccgttctt gatcagatag ggttcgtaga catcctctat cgtaccggca
     6001 tcttcaccga tggcagctgc cagggtatcc agacccacgg gacctccgtc gaatttctca
     6061 atgatcgtga ccagaatatt gcggtcgata tggtccagtc ccatcttatc gacatccatc
     6121 agatccaatg ccgttctggc tacctcctcg gtaatgatcc cgtcgtattt aacctgtgca
     6181 aagtcacgga cacgttttaa gatccggttc gccagtctcg gtgtaccgcg gcttcttctg
     6241 gccatttcct ttgctccggc cggttcgatc tccacgtcca ggatccgggc ggaatgcatg
     6301 atgatctgct gtaattcctc tatggtataa aattccagat gatggatcat gccgaaacga
     6361 tcccgcagag gtgcagtcag aagtccggca cgggtggtcg ctcccaccag agtgaatttt
     6421 ggcagctcca gacgcacaga tctggcactg gaaccctttc cgatcataat atcgatgcag
     6481 taatcctcca tggccgggta cagtacctct tccacctgac ggttcagccg atggatttca
     6541 tccacgaata acagatctcc ctcctccaga ttgttcagga tcgccgccat ttcaccgggc
     6601 ttttcaatcg cagggccact ggtcaccttc atatgcacac ccatttcgtt ggctatgatt
     6661 cctgccagcg tggtcttgcc cagtccggga ggtccataga acaacacatg gtccagtgca
     6721 tctccgcgct gctttgctgc ggtaatatac accttcaggt tctctttgat cttggactgg
     6781 ccgatatagt catccagtgt ctggggccgc agagagcctt ccagtttgat atcttcttct
     6841 gtgatatttg tctctatcat tcttctaggc ataatttatc cggttcctta aaacaatttt
     6901 ttcagcgcag ctttcagaat cgcgccggaa tccatggtct ccacatcctc aagggcattg
     6961 acagccttga ggctctctgc ctgtccgtat cccagtgcca ccagtgcttc cacggcttcc
     7021 tttttcttct cactgtcaat gtgcaggctg ccggcagtca ggattccctg ggctccgcct
     7081 gcggcgatct ctctggagat cagtgtatca tcaatggaga tcttatcttt taattccaga
     7141 atcaggcgct gcgccgtccg ggctcccact cccggggccc tggagattgc cttggcatcc
     7201 ccggacataa tggcaaaccg cagatcatct gcatccatga ccgataatat tgccagtgct
     7261 ccctttggac cgattccgct gactgtaatg cacagcttga acatttccag atcacttcgg
     7321 gagagaaaac catatagtaa aaaggcatct tccttcacat aggtataagt gtaaagccga
     7381 acttcttctc cgattcccgg cagcctggag gctgtatccg cagaaatcct tacattgatg
     7441 ccaaatccgt tgacatcgat cacagcgtta tctactgtgg tatcttccaa aaagccattc
     7501 acatatgcaa acatgtatac taagtcctcg tctttcctgt cctgcaaata gtaacctgta
     7561 aaaatcggct acgatatgtt tatcatacca tagccgaaca tatgtttcaa gcaacttttc
     7621 tgttttctgt tttccatcag ctctgaatct gtttcaactg ataaaattct ccctgcagag
     7681 ccataagttc ttcataagtg ccgtactctg cgcagcgacc gtctgcaatg accgcgatcc
     7741 tgtcggcatc ccggatagtg gacagtctgt gggccacgat aaaggtggta cgcccttctg
     7801 tcagattgtt cagcgcctgc tggatcagct tttctgaaat actgtccaga gccgaggtgg
     7861 cttcatccag tacgatcacc ttcggatccc ggatcagtgc tctggcaatg gagatacgct
     7921 gtctctgacc gccagacagt ttgccgccgt gctctcccac catggtatcc agacccttag
     7981 gcagtgatgc gatcagatcc gacagattgg cagctttgat cactttctgc agttcttcct
     8041 ccgacacatt ttcgcaaccg taagtgatat tgtcacggat cgttccggag aacaggatcg
     8101 aagtctgggg aactaccgcc aggtgctttc ggtaggtgcg tagatcgatc ttactgatat
     8161 ccttgccatc gatcaggact tttccgcttt ccgcctgatt aaaaccgatg accagattca
     8221 ggatcgtaga ctttccggcg ccggattctc ccaccagtgc aatagtctct cccgatttca
     8281 catgaagatt cagatggttc aatacattat gttcgttatt tttataatgg aaacatacgt
     8341 cctcaaaatc aaaagtaccg gtcacctgtt ccagtttttc cttgccctca ttatcttcga
     8401 tatcttcaga taacaatact tcaccgatgg aattgacgga ttcaataccc ttggcaatgg
     8461 taggcagcag ggtgacgatg gaggacacct gattgactac cgtggcaaaa taactctggt
     8521 acaatgtgat atcgcctgcc tgaattttgc cgccaatggc cagatatccg gtaaatgcca
     8581 gacagatcac ctggaagatc tggaatactg cccagccaac cgagccaaac aatgcctgga
     8641 tcagatccag tttgtagcct ttttccgcca cagcaaaaag ctggccgctc atttttgcca
     8701 cttcttcgtc ctccagggca tgtgctctgg tcacaggcac aagttccacc atttccatga
     8761 ctctggcgga agtctcttcc atttccttac ggaattcagt attccgtttt ttcatgatat
     8821 tccggaaagt gaccatggtg atggctgcta ccggtgtggt caaaaggaaa aagataaata
     8881 cgatccggct cttgctgatg gtgaccacca gcgccacacc gatattcagc gcaatgttca
     8941 gaatgcttaa aaataactgt gtggaaagcc cctctaccgc ttccacgtca cgcatgatct
     9001 tggactgcaa tcgcccggac tgagtctcca catgatagga aatggataac tgctgaagct
     9061 ttcggatcaa tgctttcctc agtcccgtct ccgcatagcg ggtggccaga ctcttgtagg
     9121 aagtatacag atagttcatc ggaatgttca gcaggatcag ccctgcttcc agaatactgt
     9181 aaaaaataat gttccgaaaa gtatacgggt tgtgggatgt cacatcattg atgatattgg
     9241 ccgtaatgat cggcagtacc cagacgcagg catgctttgc aaaaaagcag atgactgcca
     9301 gaacaaattt atgataattt cccttgtaca gatgcactaa cgtccgcaga ggatgcccct
     9361 gattgttccg gtaacattcg ataaaccagc tttcgatatc cattttctgc ctgggggatg
     9421 tttttgcttc aaaatctttc ataccgcttt tcatgccttt ccgtgatctg cgcatttatg
     9481 tcttttcctg tccgcagatc tgtttcatgt ttcaatgcca cgtttctatt ttccgggctt
     9541 acttattcat tttatcgcaa ttaatatgta ttttccagtt tgtttttgcg gttttccttt
     9601 gaaaacaact gattttttgc caaaaaaggc tgtgtcagaa cttttacatt cttacacagc
     9661 ccttttgtca tagcattgga ttttttacag ttgattagtt gttgatcagc ttatcactct
     9721 cgttgttcca gctgtaaagc ttacggatct cttcgccgac cttctcggaa ggatgctcag
     9781 cagcaagctt tctcatagcc ttgaagtgaa cctgcttgcc tgcgggagac atatctaaca
     9841 ggaattcctt agcaaaagta ccatcctgaa tatcggacag gatcttcttc atggtcttct
     9901 tggtctcctc ggtgatcagc ttggggccgg tgatgtagtc accatactca gcggtgttgg
     9961 agatagaata tctcatgcct gcgaaaccac tctggtagat cagatctacg atcagcttca
    10021 tctcatggat acactcgaag tatgcatttc tgggatcgta accagcctca accagagtct
    10081 cgaaaccagc ctgcatcaga gcacatacgc cgccacacag aactgcctgc tcaccgaaca
    10141 gatcggtctc ggtctcggtt ctgaaagtgg tctccagaac gcctgctctt gcgccgccga
    10201 tacccagtgc ataagccagt gccagatcct gagccttacc ggttgcatcc tgctctacag
    10261 cgatcagaca gggaacaccc ttaccagcct gatactcact tcttacggta tgtccgggtc
    10321 ccttgggagc gatcatggta acatcaacgt tcttgggagg tacgatacat ccgaaatgaa
    10381 tgttgaaacc atgagcgaac atcagcatat tgccctcttc caggttgggc tcgatgtcgt
    10441 tcttgtacag atcagcctgc ttctcatcat tgatcaggat catgatgatg tcagcacgct
    10501 ttgcagcttc tgcagcggta aatacttcga acccctgtgc ctcagccttt gcccaggact
    10561 tggaaccctc gtacagaccg atgataacat gacatccgga atccttcagg ttcagtgcat
    10621 gtgcatgacc ctggctgccg tagccgataa ttgcgatagt cttaccctct aataaagaga
    10681 gattacaatc ttcctgataa aaaatcttag ccattttctt ctcctccgtt atgagacata
    10741 aaatttataa ctgataggga tcttccctag agctaacata aagtgtgagg tcttacacct
    10801 gtcagtcctc aatcttcaat ataggtaacg ttctcgatac ctcttcccag acctgtgata
    10861 ccggttctgg caagctccag cacttcgtac tcttccgtaa aacgctggat cagcgcattg
    10921 actttgctgt gattaccggt aaattcaacg atcagattct ccgcacctac atcaatgatc
    10981 ttcgcacgga aattgttggc aaggaattct accccctggc gaatctccag acgcttttcg
    11041 ggatctactt tcaccttgac cagtaccagt tcgcggtaca cgctgtcatc cgggtccagt
    11101 tccttaatat cccggacatc cacaagcttt cccacctgct tctcgatctg ttccagaata
    11161 tcgtcatctc ccgatgtcac aatggtgatt ctggtaaaac ggggatcggc ggtcacacct
    11221 gccgtaatac tttcgatgtt gtaaccgcga cgggaaaaca atccggcaat acggctcaac
    11281 acacctgaag tgttgtcaac caacacctga aatacttttt tctgcatgaa tatctaccat
    11341 acctttcgta tttctacagc cgtcctccct gacggagcag caactgtatt cttaatatag
    11401 caagatgact acagaatgtc aattagtgaa tttaacatta tgattgatgt attttaatca
    11461 tcattttccg tgcattttga taatgccaag gttcctgttc gagctacttc cacaatactg
    11521 atggtctgaa gcatcttgat cagagtgtcg attttctccg gagcatcgca aatctgcagc
    11581 atcataaagt gttttgacat atctacgatc tctgttttca taatgtcaca gatttctata
    11641 atatccctac gattattctt gttatattta acttttaata acattaattc cctggtatag
    11701 cactgttcct gggacaaagt cttcactttg atgacatcca gtttcttatt cagctgcttt
    11761 tcaatctgct ctagggtacg ctcatcaccg gagcttacaa tggtcataca ggaaactgca
    11821 tggtcatcgg tctctcccac ggcaaggctg tcaatattaa aacatcttct ggagaacaga
    11881 ccggctacct tgcacagaac accggaacgg ttctctacca atacggaata tattctcttc
    11941 attttaagta cagctccctt ccgttatttt accagatcca tcggatcgat cagacattct
    12001 aacagcatgg tattgtcttc cgccagaaaa gcgtccagaa tctcatccac gtgttccatg
    12061 ttgttcaatc gcagatacgg aatatcatag gccgcagata atttctccag atccgggctt
    12121 ccggacagat ccacgacaga ataatgatct ttgtaggtat agtgctggta ttcccttacc
    12181 atacccagat aattgttctt tagcacgatg atctttaccg ggatattgtg ctgtctgatc
    12241 gtagccagct ccatcatgga catctgaaag gaaccgtcac cgcataccgc aataacctgc
    12301 ttatccatgg ccgctgtctt ggctcccatg gctgccggaa tagaataacc catggttccc
    12361 ataccgccgg aggtcaggaa tttgcctttt ttcacaatgt gatagccgca ggaccagatc
    12421 tgattctgtc ccacgtccgc cacatagaca ccgtcctcct gcatcttctc ggaaagtctt
    12481 gtgataaatg ctgcaggatc cacataatcg ggattagggg ttctcttctt ctccatggta
    12541 gagcgatatt cattcaatgt agtgagccat tcctcgtagt tacagtcaaa ttcttctttc
    12601 tgaaaatcct ggaagatgtg ttttgcatca cctaccagtg gaatggaagg acccgcattt
    12661 ttgccgatct ccgcaggatc cacgtcaatg tgcaccagca ctttgtttct tgtgatgaga
    12721 tccggctgac tgaccgcgcg atctgccact ctggctccca ccatgatcag cagatccgat
    12781 tcattcatgg cacggttggc ataagtctta ccgttattgc ccaccattcc ataataaagc
    12841 ggatgttcgg taggcataac accgataccc atcatggtgg acaccacggg aataccgtgt
    12901 tcttccgcaa agctgcgcag ctcctcctcg gcctcactga gcaggactcc tcctccggca
    12961 cagataatgg gacgtttcgc ccgctccagc tctttgataa ccttcttgat ctgcacggca
    13021 tgtcctttta cggtaggctt gtaggtacgt agattcactt cttccggata atggaaattt
    13081 ttgatcgttg cattctggat atcaatggga atatcgatca ataccggccc ttttctaccg
    13141 gtattggcaa tataaaatgc ttccttgaac actctgggaa tatcatttac attttt
//
LOCUS       DAWDHD010000064        13103 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_308_length_13103_cov_14.407419, whole genome shotgun sequence.
ACCESSION   DAWDHD010000064 DAWDHD010000000
VERSION     DAWDHD010000064.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 13103)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13103)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13103
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_308_length_13103_cov_14.407419"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3625 a         2828 c         3582 g         3068 t
ORIGIN      
        1 aagaacagga tcagtatttc cagagagata caaaagtgat aaaataggat caataaggat
       61 tttttcagta taaacttttc acgcgcaaat tgtgcctgca cagtgtacgg aagtacaggt
      121 ttaaagcacc ggaaggcttg caggctacgg aaagacaaga gggttaaaga gatgaacgaa
      181 atgatcagaa aaagaaatga acaactggca aaaacagtca tcaagggttt acagtcccgc
      241 aacatgtcgg gctactacgc agaggataaa gaagccgcgc tgaagcaggc actggagctg
      301 atcccggagg gaagcaccat tgccatgggc ggatgcatga gcgccggaga gatcggactg
      361 atctccgctc tggaagcagg caattaccac tatatcgacc gctctaagat ggatgccaga
      421 gcaggactga tggcagcata cgatgcggat atcttcctct ccagtgccaa cgccatcacc
      481 agtgacggtg ttatggtcaa tatcgacggc aattccaacc gtgtctcctg catagcccag
      541 ggaccgagga aagtaatctt catcgtcggc atgaacaagg tctgcgcaga cttagacagt
      601 gccatgaagc gtgcgagaaa cgtggctgca cctatcaatg cccagagatt cgacatcaag
      661 actccctgca aagaaaccgg aaaatgcttc gactgtaagt ctccggatac catctgctgt
      721 cagttcctga tcacaagata ttccagacat acggacagga tccatgtgat cctcgtaaac
      781 gacaccattg gttattagac aacaaagggg aaacgacaat gacattagta ttgcaaaaag
      841 gaagacctgc agatacgacc ggcagactgg acaaagagat ccgtacctat gacctgctgg
      901 acaggctcgg ggtggaatat gaccgcgtgg atcatgctcc ggccatgacc atggaggact
      961 gcaaggaagt ggatgagatc ctggaggcta tggtatgcaa gaatctgttt ctgtgcaatc
     1021 gtcaggagac cgcattttac ctgttgatga tcccggatac caaggtcttc cataccaagg
     1081 atctgtcggc acagatcggc tcggcgaggc tttcttttgc caagccggaa tacatggaga
     1141 aattcctgga tatcaccccc ggttctgtca gtgtactcgg tctgatgaac gataaggaac
     1201 atcaggtgaa gctgttgatc gatgaagagg tgctggacag cgaatatttc ggatgccacc
     1261 cctgcatcaa tacctccagc ctccgcatgc ggacggcaga tctgatcgaa aaggtgcttc
     1321 ccgccatggg acatgatttt gtaaaagtaa agctgtaaaa agtaataata taagcagatc
     1381 tgcccgccgc cggaatcccg gcagcgagga tctgtaagcc tggttttgca tggctctgaa
     1441 tagttacaag atattaaaat acctgttcgc actcttcaca catcagcagg aaggcgccga
     1501 ccccgtgcag gtcgttggcg gatacgggac ggtggatata gtggtcataa tcaccgacac
     1561 cggttccgat gcagacatta ccgataatga ggtcatcacc ttcccaggtc agggagcgga
     1621 tcacaccctc ataacccttg cgggcatatt ccagataaga tgcatccagc agacctttgc
     1681 ggatcgcttt gcacagggcg gcaacataca ggcagctgca ggaattctcc agccagttgg
     1741 caggatcatc gccccggttg actacctgat accatctgcc gtcttcagac tggaatgcga
     1801 gcagagactt taacagatcc actgccattt tttccagttt tgctctgtcc ggatgatcgg
     1861 cgggaatgaa atccagttcg tccaggaggg ctacaggcac ccatcccatg gctctgcccc
     1921 agaattcggg ggagcagccg gtctccttat cagcccattc tactttcctg tcataggacc
     1981 aggcatgata cagcagaccg gtcttggggt cacgggtatg tttctgcatg gtcagagcct
     2041 ggaagataac ctcatcaaaa tattcggggt gatggaattc tgcgccatac tgcgcgcgca
     2101 gcggaccttc catgtacaga ccgtccagcc acatttcttc cggcagatca gccttgtgga
     2161 aatagccgcc ctcgggagtg gtgggataac gatcaatggc agccatcaga gagtccaggg
     2221 ctttcttata tctggcatca ccggtacaat gatagagggg atataacaga atacccggct
     2281 gcatatcgtc gaactgcccc atatcagcct gtttgatcac accgtcttcg gtgatgacgg
     2341 catccaccca ggatttgatg taatcaaaat atttttcctc tttgcaaagt tcatagcttt
     2401 tgtacattcc ggacaggaat actccctgat gataatggaa tcttccttcg ggtggaagct
     2461 tgggggcttc ccatttgcgc atcatggtat ccatggatgc ttttgcatag tctaaaggtc
     2521 ttttctccat aagttatctc cttcccatat acttcacatg gcacaagatt tattatataa
     2581 tcccattata atcttttatt tttaagattg tttaacatat cttgcggtaa taaaaagcaa
     2641 tcgaatgaat cttgccggaa tgattccggg aaaggaaaac tatgcttcgt acgatttttg
     2701 acccttattc cgatctgttt ctggaataca tagagacaac caaaaaaaat gatatgcaga
     2761 ccaatcatta tcacgatact tacgaacttt tttttgtggt gaaggggata cgatacatct
     2821 tcctggatgg tgtctgtcat gtgctgaatc cgggagacat tatcctgatc aaaccctacc
     2881 agacgcatta tatgcagagt ctgtcctcgg atttctatgc ccggtacgtc ttgaactttg
     2941 caccggatta tctggatgga atgttgaaac ctgcggagaa gcagcggttc ctggaggttc
     3001 tgcagaatga tattctgcat ctgacaacgg aacagtccgg agaactggta gaggtcttcg
     3061 aacggctgcg gaaatattat aaccgccacg atgctgtggc cgagaaatta aagcaaaact
     3121 attgtctgga actgctgcta ctgtgcagag actactatac gaggcaggag cggacacaga
     3181 tgccggagct ctccgcagct cccagacagg agattctgga tgccatcgcc tatatcaatg
     3241 cccattatgc ggaaaaaata gatctggaga ccatcgcatc ccagatccat ttaagtaaat
     3301 actatttttg ccgtattttc cgcgaggtga ccggagccac cgttctggaa taccttacca
     3361 atgtccggct gaccagagcc ggaagacttc tggtggagtc ttctctggcg atccaggaga
     3421 tcgcgcagaa gaccggattt gcgtcattgg tgcatttttc cagaacctac cgcaatgctt
     3481 atggtgtcag tcccagtgat accagacgcc ggcgaaaggc gtagaagcta ctgaatccca
     3541 caggcgagac atgctgacat ttgtgacgca aaaagtcaaa tcatccacaa attgatttct
     3601 cgttgagagt cttatataat aaagtcgagg tcaaaggccc ccggctttga tgcctacgtg
     3661 ctacgcactc ccacaatcct accctctatt cgcatatcgg ggtgggtcat ataacagaaa
     3721 tggtttatac cgtaaaaaat gtatggtaag aatacgaaag agagaagaaa atggtactac
     3781 agatattaac atatacacat catataacga caatgctgtt tggaatcttc ctgtcggcat
     3841 tttttctggg agtgaagcag gataagaaaa atatattgca attgttgtca ttggcggttg
     3901 tttcaggaat tctatatgtt ctgtgtgtac ttctctttgg aacagaaaca gtagatcaga
     3961 tttatccact gatcgtgcat gccccattgt tatttgtact ggttttgcat tataaattcc
     4021 gtattcttcc ttccctgatt tctattttca cggcatatct atgttgtcag tgcagtaact
     4081 ggatgggact gtttgctttg gctctgacag gacaggaatg gtgctattat gtctgtcgta
     4141 ttctggtgac ggtgggagct ttcatccttt tatgccggta tgtatgccag accacagcca
     4201 tgctgtttgc caaaacggac cgggaacttc tgatcattgg aagcctgccg atcatttatt
     4261 acatttttga ctatgccacg accaaatttt ccagtctgtt gtattccggc aataaggcgg
     4321 tgccggagtt cctgggcttt gccatgtgcc tgacgtatct gcttttttta ctggtatatt
     4381 tccgggagta tgagctgaag aataaggcgg agcagtacaa cgaaatgatc cggatgcagt
     4441 taagttctct gcatacggaa atggaaagta caagaaaaag cgagcagaga atgtccatct
     4501 tgcggcatga tacaagacat catctgtcgg cgatccggac actgatccag cagggagagt
     4561 ccgacaaggc gctggaatat ttgaacgaag tgagccagac ctatgacgat acggtgatca
     4621 agacctactg taaaaatgaa atggtgaatt cggttctgtc catctataac tcccggtttg
     4681 aggaacggag gattcactgg gaagtgcagg tcagtatcgg ggagaagctt ccctgttcgg
     4741 aaatgatgtt ctgtgcgatc ctgtcaaacg tactggaaaa tgccatgcat gccgtacagg
     4801 aactgccgca ggaaaagcgg ctgatacagc tggctctgtc cgaaaagtca ggacatcttc
     4861 ttctgatggc gaaaaatact gtgcagaatg ccccggtatt tattgacggt gtcccggtga
     4921 cggagcgctc cgggcatgga ctgggggcca gaagcatcgt atattatgta gaaaagctga
     4981 acggacaata ccagttcttt atggagaacg gggattttgt ggtcatgatc atcttgtaaa
     5041 atcaataaaa tgtttatagg gctctttctg tcagagaaag agcttttctt gtggatgtat
     5101 tgggaaatta atgctattat taggataaaa gatacagaaa tatagatata acaggaaggg
     5161 agcagacaga tatatgaaat atacattgga ttatgagaaa tatgcggaac tggcaagacg
     5221 tgtggcggcg gagggaagcg tactgcttcg aaatgaagac ggtgtgctgc ctctgcaaaa
     5281 aggacagaaa gtatcgatct tcggaaggac acagttcgag cattataaga gcggtacggg
     5341 ttccggaggt atggtcaatg ctccatatgt gaccaatatt acagacagtc tcaaggaaga
     5401 cggcaccgta caggtgaatg aggtactgga ggcacagtac cgggaatggc tgaaggatca
     5461 tccttttgat ataggagagg gatgggctat ggagccctgg aaccaggaag aaatgcccgt
     5521 cagcgaagaa ctgacgagac aggcggcgga acagtcggat cttgcgattg tggttctcgg
     5581 ccgaaccgca ggagaagaca aggataatag tgctgcggaa ggcagttatc tgctgacagc
     5641 tgcggaggaa gaaattcttc gtaatgtctg caatgcattt gccaggacga tcgttgtgct
     5701 gaatgtgggt aatatcatgg atatgaagtg ggtcgaccgg tatcagccac aggctgtgct
     5761 ctatgtatgg cagggcggcc aggagggcgg cagagctaca gtagatgtcc tgacaggaaa
     5821 agtgaatccc ggtggtaagc tcagcgatac tattgcggaa gatattgagg attatccttc
     5881 cacggagaat ttcggggatc cggtggagaa tttttatacc gaggacatct atgtaggtta
     5941 tcgctatttt gagacctttg ccagagacaa ggtaaaatat ccctttggct ttggactgtc
     6001 ctatacccgg ttccagacag agagcatggg actggcagtg cagggtaccg aggcaactgt
     6061 cattgagaaa gtgacaaatg ttggcggtat gtccggcaga gagaccatac aggtctatgt
     6121 ggaggctcct cagggaaagc tgggaaagcc gctgcgtcag ctggcagctt atgccaagac
     6181 ggaagaactg aagcccggag caagtgaaga actgatcctg aaagcagaac tgacggagct
     6241 tgcgtcctat gatgacagtg gtgtcactgg acataaatcc tgctatgtat tggaagcagg
     6301 agactatatc ttctatgtgg gcacagatgt gcggagtgca agagaagtcg gaagagtgac
     6361 attagcggag ctgcaggtcg tacggacagc tacagaagca ttggcaccgg tgcaggcatt
     6421 taagcgcctg agaccgtttt tcttcggaga gaatgaccat gccatcgcca actgggagga
     6481 tgtgccctta cggaccatag atctgacgga acgtattttg cgggaaagac ccacccagag
     6541 cgaatatgtg ggagatcagg gctggaaact ggcggatgtc tatgacaaaa agattacatt
     6601 ggaacagttc ctgacacagc tgtcagacga agatctgatc tgcatgacca gaggagaagg
     6661 catgtgttcc ccgaaagtta cccccggaac agcagcagct ttcggcggtg tgacggatgg
     6721 actgcagcaa ttcggtattc cggtggcctg ctgttccgat ggtccttccg gcatccgtat
     6781 ggactgcggt accatggcct atgcactgcc gaacggtacg ttgctggcat gtacctttga
     6841 tccggagttg gtggaagaac tgtatgagat ggagggcatg gagcttcgca agaacaagat
     6901 tgattccctc ttaggccccg gcatcaatat tcacagaaat ccgttgaatg gacgtaactt
     6961 tgagtatttt tccgaggatc cgtacctcac cggtaccatg gcggcggcac agctgaaagg
     7021 catgggaaaa tatggagtta ccggaaccat caagcatttt gcatgtaata atcaggaatt
     7081 caaacggcat gatgccaatg ctattgtatc cgagagggcg ttgcgggaga tctatctgaa
     7141 aggatttgag atcgccgtaa aacagggcgg agcctacagc atcatgtcca cctatggccc
     7201 ggtgaacgga ctgtggaccg caggcagcta tgatcttctg acaaccattc tccgtaagga
     7261 atggggctat cagggtattg tcatgaccga ctggtgggca aagatcaatg aggaaggcga
     7321 atccggttcc aagagtcaga cggtacctat ggtccgggcg cagaatgata tttacatggt
     7381 gaccgcagac gcagcatcca attctaataa ggacaacact gcggaaggac tggcagacgg
     7441 caggctgacc agagcgcagc ttatgcggaa tgctgcaaac atctgctgct tcctgttacg
     7501 ttccgttgcc atggagaggc tgcttggcag agaggaagag gagtgtacgg agcttcacag
     7561 tccggtaagc accgaaggag ccggagacag cggacaggtg ctggcccggc tggaactgcc
     7621 ggagccgaag accggcgaag agattgaact tccgctggag aagctgtcca cgaagaaagg
     7681 tactggtgcg gtataccagc tgcgtttcac gaagatcgga cggtacgaac tggtattccg
     7741 gatgagttcc acgctgggtc cgctggcaca gttaccgatt tccgtattct tgaacaacac
     7801 gttgcagcaa actgtgacga ttaacggcac ggagggcaaa gtggtggaac agtccgtcac
     7861 gctggcaatc cggcaggacg gtgagaagta catgaaattg tatttcggag aaagcggtat
     7921 tgatatgcac cggatgttct tgcggtatgt gggaaagaac gatatcgaaa gcttccggaa
     7981 tgaataaaac aatataagca ataaaaaggc tccgaaagat cggtatgcag gcaggatctt
     8041 ccggagtttt ttatgcgtcg caatttgtaa aaaaacttta acggaatatt tatgcataga
     8101 aaccaaaaga agaattgact tatgccgata tttcggatag aatctactct tgtatgttct
     8161 aaagaaaaat tagcaaggag gcagggaaag tggtagcaca aatacttgca gtaatcatat
     8221 ttctggcgat gtttgtactg atcgtcctgg aagtatggga gagacacatt atcacattgg
     8281 gctgcggcct gctgacactg gtactggtat tcggactggg aatgcacagt atgagcgcag
     8341 tgctggagac tttgaactta ggcagctttt tcacaagtca tttctggtac acggcgggac
     8401 agtccgcaga ggcttccagt ggtatcaact gggagaccat cgtgttcgta gcaggaatga
     8461 tgatcatggt agagggaatg gcccgggtgg gattcttccg ctggctgtgc atgcgccttg
     8521 ccaagatggt caagtataag gttgtaccgc tgtttgtcac ctttatggta ttatccggta
     8581 ttctggcaat gttcattgac agtattaccg taatcctgtt cctggcagcc gttaccattg
     8641 aattgtcaca actgttgaaa ttcaatccgg tgcctatgat tctggcagaa gtgttctgtg
     8701 cgaatctggg aggatccgct accatgtgcg gagatccccc caatatcatc atcggtactt
     8761 ccctgggata ttccttcacg gatttcctga ccaataccgg tgtgatcgca ggcgtttccc
     8821 tggttgtggt cgtactgtat ttctatctgg tattccacaa ggaactgcgt gccagtgagg
     8881 cagctgcagc aggcagcaac cagacctacc cggatccttc cgaagccatt accgataaaa
     8941 aaggatttat tatcagcact attattttcc tgtgtgcggt agttctgctg gtgacccatg
     9001 cacagactgg actgaccgta tcctgcatcg gtgtattcat ctccatcgtg acattgatcg
     9061 ctgcaggcaa agatgcgctg aagctgatca gacagattga ttataagacg ctgctgttct
     9121 tcatcggact gtttatggtc gtaggtggtc tggagcagac aggtatcctg aaagtcatgg
     9181 cgaacttcat cggagacatc agcaacggca atctgatgct gatgatcgct attatcctgt
     9241 ggatctctgc gattgccagc gccttcgtgg ataatattcc ttttgcggct accatgatcc
     9301 cgattatcag cagtctgtct gctgcccaga gcgtggatct gtccattctg gcatgggcat
     9361 tggctatggg aaccgatatt ggaggaagcg caactcccat cggagcttct gccaacgtag
     9421 taggtatcgc caccgcagcg aaagccggtc atatgattaa gtggggaaaa tattgcaaag
     9481 ttatggcacc tgcgactata atagtagtag ggatctctat gctgatgatc tatgcaagat
     9541 atctgtaaga tgtatttgca gaatacgagt ttaaaaaatt ttggaaatac atccagccat
     9601 aaggagggca gatccatgag tgaacaactg attatctccg tcagtcgtga atacggcagc
     9661 ggcggacatg agatcgcaca gaagctggca gagtattata agctgccgat gtacgatcac
     9721 aacctgttag acgaggtagc tgcaagcatg aacgtaagca gccaggagct ggcagagttc
     9781 gatgaaacga gacgtaacaa attcctgtac cgcagtgtaa tgggaatgaa cagctctccg
     9841 gcagacaatg tggccagaat gcagttcgat tatattaaga aaaaggcgga agcaggggaa
     9901 tcctttgtga tcgtaggacg ctgttccgag attgtgctga aggacaatcc gcacctgatc
     9961 tccatcttcg tattgggaga ccgggaggcc aagatcaaac gtgtcatgga gatctatgag
    10021 ctggatgccc gtcatgcgga agagcgtatg atcgagaagg accgtcgcag aaagtcttac
    10081 cacaatagtc attgtaaggt aaaatggggc gattcccgta attacgatct gtctatcaac
    10141 agcagcaagc tgggtgtaga agagacggtg gaatctctga aaaattacat agacgcaagg
    10201 cttgctcata aataaagcag acataagtca acaaaaatgc agagaccatc tgaggaaagc
    10261 atacgaaaga aaatcgtgta gctgccgcag atggtctttc ttgttggcgt ttcttaagaa
    10321 ataccggata acatttaaac aaattttaag gatttctgtc gtgacgaata cagcagatta
    10381 tggtattatc ctagaagatg aataggaata aaagagtgca aaatcaggta tgtggaagtg
    10441 aggtacagga tgagcgataa ggaaagagac tccattcagg agatggaaga tatacaaaag
    10501 ggtctggaag cctttttaca acaggagatg caggaaatgc aggcatccgg ggcgggagcc
    10561 cgccgcagta cggggcgccc ggcggcgaat gcggggacac actctgcgga gcgtgaggca
    10621 gcccgcccgg ctccgcagat gtatgatatg agggaagatg acgaggaaga ggaggaactg
    10681 gatgtgatcg gatccgacag aagagcagga tcccgtgagg actatgcaga ggactgggat
    10741 gatcccacat accgcaggag acaggcagcc cgcagagaga cggcacgtaa gacttcagag
    10801 agtcgtaacg gtggcggccg tagcagtaac gctgatcacc gcagacagga gacctcaggc
    10861 agaacccgcc gcagcagacg ggatgactat gtagaggact atgaggacga ttataacgat
    10921 agaaaacgct ccggaagaag atccggaaag cgtgaggaga tagacccgaa agcggataaa
    10981 aaaagcagaa aaggaaataa aaaggtcgct aagggcggca ggaagaagaa aaagttagga
    11041 ctcaagcgtt tcctgatcgc agtggcgctg atccttgtat tcctggcagc aggattgtat
    11101 gtgctggtag gaaaggtcta cggagagatg aactatgaga agattgagtc tgtagctgct
    11161 tcccccatga aggaagacgg tgtgaccaat atcctgctga tcggtaacga ctcccgtgaa
    11221 aacggcgagg acggaagatc ggatgccatg atcctgctgt ccatcagcaa caagacgaag
    11281 aagatctaca tgacatccct gctccgtgat atgtatgtgg acattccggg acataagggg
    11341 aatcgtctga atgcggcata ttcctatggt ggcgcagaac tgctgatgga gactattgag
    11401 cagaatttcg atattcatat cagccgctat gtactggtga actttgaagc atttgccaat
    11461 ctggtggatg cagtgggcgg tgtggatctt gagctgacca gcaaggaagt ggaatatgtc
    11521 aatggttatc tggtggaata caacattctg ttgggaagac cggaaggcac cgattatttt
    11581 gaagatacct ccggaggcat ggtacatctg aacggaccgc aggcactggc gtattgcaga
    11641 aaccgttata taggtacgga tttcggcaga accgagagac agagaaaggt attggcagag
    11701 gtgatccaca agcttccgca gggaatgctg accaatcccc aggagctgat cgatggtctg
    11761 atgccgaatc tgaccacgaa tctgacccag acagaatgct atcggctgag ccttatggca
    11821 cctaaggccg tgacttacga tattatccag aacagcattc cactggaggg cacctataag
    11881 gatgcgacca tccgtaagat ggcagtgctg gaagtggatt tcgaagccaa caagaaattc
    11941 ctgcaggaga atctgtacgg agacggagac agtacttcca catcggaggc ttctgcggaa
    12001 tgatattata attgacgtat gagagtcaat gtgtcagcag gattgacgaa cggcgaatgg
    12061 gatcatgata gttgcgaagc aacggtaagc accaaagatg cggatcatgc tacgataatg
    12121 agggagcagc ttatggggta tcgaaaacaa aggtttgcag cactgttctg tgcagcggca
    12181 gtcataatac ttggtggatg tggcgatgat ctgacccagc aggtctacat tccgggaagg
    12241 gaagatctgg cgcagcctat cggcggttac aatgcaacta cgcaggctgg gcagacggca
    12301 gaagattctg ctacgggggc acagacaggg acggcttccg gtggagagaa cgcaacaaca
    12361 tcgggatctt gccatgactc cggtgatttg acgggtgaac gctatacgat caccatctcc
    12421 gcagcagggg atgtaacgtt gggaaatcat caggaacaag actacagtgc ctccttccgt
    12481 caggcctatg atcaggcgga ggacgaaggt tatttctttg aaaatgtgag agacatcttt
    12541 gcggcggacg atatgaccat cgtgaatctg gaagggcctc tgaccacatc ggaacagatg
    12601 cgggagggac agacctactg catcaaaggg gatccagcct atgctcatct gctgacgctg
    12661 gggagcattg aggccgtcag cttcgccaac aatcaccgcc tggattatgg cgaacaggga
    12721 agccgggata ctgtggtggc attagagcag gaagggatca tttatgctta tgataaaaat
    12781 gtgggtatct atgagatccc ggacagtgcg gcagcccacg gcggagaacg gaacctgaaa
    12841 ataggaataa tctctgtcaa tgaggtagag tggggcgcac aggcggaaaa gctgatccag
    12901 aacaatattg aaactctgcg ggaacagaat gtggacctga tcctggcctg ttgtcactgg
    12961 gggatcgaga aagatactta tccggagaat taccaacagg tacttggcag aaaatgcatt
    13021 gactggggtg tggatctggt cattggtcat catccccatg tcctccaggg aatcgaagaa
    13081 tataagggca gatatattat cta
//
LOCUS       DAWDHD010000065        12677 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_319_length_12677_cov_9.341071, whole genome shotgun sequence.
ACCESSION   DAWDHD010000065 DAWDHD010000000
VERSION     DAWDHD010000065.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 12677)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 12677)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12677
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_319_length_12677_cov_9.341071"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3812 a         2658 c         3373 g         2834 t
ORIGIN      
        1 tacgctcccg gcagcgagaa acacaaggca gaaactctcc agaaaggcca tgcacatgat
       61 ctcactactc tgtagcctac cggaacggtg ttcgaccggt aataactttt cctgcgatag
      121 aagcggcggg gatgatcccg acttccgcaa agcggctgtc aatgctgtgg ttgcggttgt
      181 cacccaggac aaaatattct ccgggtgcaa gcgtcagggg agcggcggct ttgccggcat
      241 cctccatgag agaaaagcca tcgggggtcg gttggggaac cccattgaca tagaggattc
      301 cctctgtgat ctgtaccgta tctcccggca gggccacgat ccgtttgacg atatcgcgtc
      361 ccagcgtctc gtggcggatg acaatgacat cgttatagtc cagacagtct ggcagaccga
      421 atttctgaag gaagaccggc tgcccggagg tgtaggtcgg ttccatggaa gaaccgttta
      481 caaaatacag ctgtaccacg aaatgggaca ccagataaca gatacagccg ataagcaata
      541 caaaaagcag ataagaacgg gtgcgatgat tcataaggga tcctttccgg tttgttaaaa
      601 tgctgataaa aatatcaaaa tctatcagat agcataccac atttcgggtg gtaataacag
      661 agaagagaaa caattagaga aaaagaggta aaagcaatga aagtattact ggtaaacgga
      721 agcggacatg cgaagggctg tacctacaca gctctggagg aagtagcggg agcactggag
      781 aaaaacggta ttgagacgga gatcatccag gtgggtacac agcccatcgc cggatgtatc
      841 ggctgcaatg cctgtctgaa gaacggaaag tgtttccggg aggatggcgt gaatgaattt
      901 gtggaaaaag cgaagactgc agacggattt gtcttcggat ctccggtgca ttatgcatct
      961 gcttccggta tgatcacttc cttcctggac agagcatttt acggcaagag cgccctgttc
     1021 cagggcaagc ccggtgcctg catcgtcagc tgcagacgcg gtggggcttc cgcagccttt
     1081 gaccagttga acaaatattt taccatcaac tgtatgccgg tggtatccag ccagtattgg
     1141 aatcaggtac acggcaatac tcccgaagag gtaaaacagg atctggaagg catgcagacc
     1201 atgcgtaccc tgggtaacaa tatggcatgg ctgctcaaat gcattgccgc aggaaaagct
     1261 gcgggcgtgg atttcccgga gagagaaccg gcgcagaaga ccaactttat tcgataagga
     1321 gacagatgaa ttatggcaga gtggaatgga ttgggaagat atccggagga tgcggatgtg
     1381 aatgatcgtc cggaagaggg taccttgctg gcctatgtgg acggaagcta tcaggatgca
     1441 ctgaaaaaat acggtttcgg ctgtattttt attcttcccg acggacgcat ctatacagaa
     1501 tacggtaatg gtgacaatcc ggactctctg aaacagcgga atgtctccgg agagatgctg
     1561 ggagccatgt atgcggtacg gtttgcctta aacagcggct ttacagtcat cgagatccgc
     1621 tacgactatg aaggaatcga gaaatgggtc accggtgcct ggaaaagtaa gaatgatctg
     1681 acgaaaaaat atgcacagac catgctgggc tggggacaga agattcagat ccgctttacc
     1741 aaagtaccgg ctcattcgaa ggtaaaatac aacgagctgg cagatgagac cgcaaagctg
     1801 ggtgtggcaa acggaaacgg tattcccaaa gtcaaaagtc tcagtgactt cgaagcggca
     1861 gacggtgtgg aagcgtgaaa acatatactt gcggccgtgt gtctgtagcc cggaagttaa
     1921 gggcttcgga aaggaatgag gaatagaatg cgtctgaata aatatctggc acagtgcggt
     1981 gtatgttccc gaagagatgc ggataagctg atcgaacaag gaaaagtact ggtcaacggt
     2041 caggtggctg gaatgggaca acaggtggaa gaaacggaca ccgtgcaggt gggtaagaag
     2101 attctgcagg gaaaacagac acagaaggta ctggcatttt acaagccggt gggagtgacc
     2161 tgcacggaga gagatcctca tgcagagaaa aagatcatgg atatgatccg ctatccggaa
     2221 cggctgacct atgcgggacg tctggacaaa gattccgaag gcctgatcct gctcaccaat
     2281 gatggagatc tgatcaatgc catgatgcgg ggcgccaacc gtcatgaaaa agaatatatc
     2341 gtaaagacag atagggaaat aacacccgat tttttggaga aaatggcgaa aggtatttat
     2401 ctgaaagatc tggacatcac taccagagaa tgtgagattg aacagatcgg aaaatttacc
     2461 ttccgtattg ttttgactca gggagtcaac agacagatcc ggcggatgtg tgaagcctgt
     2521 ggataccggg taaaagcgct gaaaagaatc cggatcatga acattctgtt gggggatctg
     2581 aagccgggaa agtggcgcga ggtcaccgga gaagagctgg aacagcttca aagagacacc
     2641 ggaatgtaaa atcgcaatta aataatataa aggcaggaat acaagatgca gtcttccaaa
     2701 atagaccgga tcaaagatct ggtagaacaa ctgaatgcag cttccgaaag ctattacgcc
     2761 aaggatcagg agatcatgag caactacgaa tacgacaagc tctatgatga actggtggaa
     2821 ctggaaaaag agacaggcgt taccctggcc aacagtccta cggtaaacgt aggctacgaa
     2881 gcggtggatg agcttcccaa ggagcgccat gagagtccta tgctgtctct gggcaagacg
     2941 aaaagccggg aagaactgcg ggactggctg caaggcaatc ctgcaattct tagctggaaa
     3001 ctggacgggc tgaccattgt attgacgtat cgtgaaggaa aacttgcaaa agcggttacc
     3061 cgtggaaatg gcgagatcgg tgaggtcatt acaaataacg cccgtacttt taagaatctt
     3121 ccgctgaata tttcctatac cggagaactg atcctgcggg gagaagcggt gatcacctac
     3181 agtgattttg agaagatcaa cggggagatc gcagatgcag aggcaaaata caagaatccc
     3241 cgaaatctgt gcagcggctc tgtgaggcag ctcaacaatg agatcacggc cagacggaat
     3301 gtccgctttt ttgcttttac gctggtaaag gcggatggtg tagattttca tgattccaag
     3361 gaagcccagt ttgctttttt gcaggagcag ggctttgcag tggtggaaca tgtggcagta
     3421 acggaagaga acattttatc tgccattgaa gattttgaac acaagatcga gcactacgat
     3481 attccttccg acggtctggt acttacttat gaaagtatca gctacggaca gtccctgggc
     3541 agaactgcga agttccccag agattccatt gctttcaaat gggcggatga actgcgggag
     3601 actacattaa aagaggtcga gtggagcgcc agccgtaccg gtctgatcaa tccggtagct
     3661 atttttgagc cggtggaact ggaaggcaca acggtgagcc gcgcttccgt gcataatatc
     3721 agtatcatga gatccctcag actgggaatc ggagaccata tcacggtcta taaggccaac
     3781 atgatcattc cccagatcgc tgagaatctg acaggcagtg ataatgtgga gattccaaag
     3841 gtgtgtccgg tgtgcggaca gcccacggag atccgacaga tgaatgaggt acagtctctg
     3901 tattgcacca atgagaaatg cccggccaag gagatcaaat cctacactct gttcgtcagc
     3961 agagatgcgc tgaatattga tggactctcc gaggcaacat tggagaagct gatcgatcag
     4021 ggctttatcc atgaatatgc ggatctgttc cggctggacc gttacaagga ggtcattacc
     4081 ggaatggaag gattcggaga aaagtcttat cagaatatga tggacagcat cgagactgca
     4141 agacatacca cgctgccccg gttgatctac ggactgggga tcgcaggaat cggcgtggct
     4201 aatgccaagg tgctgtgccg ttattttgat tacgttctgg aacgaatgca ggcagcggac
     4261 gaagaaacac tgagtgccat tcccggtgtg ggagaggtgt tggcatcggc atttgcggat
     4321 tatatgcgtg atgcggagaa tctggagaaa atagctcatc tgaaggaaat cctgacgata
     4381 gagacaccac agatcgatga gagtgcacag acactggcag gaatgtcctt cgtggtgacg
     4441 ggaagcctga accattatgc cagccggaat gatctgaaag aggtcattga ggaaaaaggc
     4501 ggcaaggtga ccggaagtgt caccggcaag accacctgcc tgatcaacaa tgatattaca
     4561 tctacatcat cgaaaaataa aaaagcaaaa gaattacagg ttcccattct gacagaagag
     4621 gaatttctga gaacctataa tattccttac gaggaataag caagaaagac ccggaggaaa
     4681 ataaaatgcc tatcaaaaca caaaatgatc tgcctgccaa agagatactg gaaaatgaaa
     4741 atatattcgt catggacgaa tttcgtgcgg tacatcagga catgcgtccc ctgcaggtac
     4801 tgatcctgaa caatatgccg gtaaagcagg atacggagct gcagttgctg cgggcgttgt
     4861 ccaatacacc actgcaggtg gatgtcactt tcatgaacac caagacccac gtgtcactga
     4921 ataccccggc cagccatctg aacaaatttt acactacctt tgatgagatc agggatcgta
     4981 aattcgatgg actgatcgtt accggagcac cggtggagga tattaccttc gaggaagtgg
     5041 attactggga ggagacctgc cagatcctgg actgggcgga gacccatgtg acctccacct
     5101 tacatatctg ctgggcggca caggccggat tttatcatta ttacggaatc aacaagagac
     5161 aattgtccca gaagctgttc ggcgtctatg cgcataaggt atccaaccgg aaagttccgt
     5221 tggtccgggg atttgatgat attttcatgg ctccgcacag ccgtcatacc gagactccgt
     5281 cggaggccat tcatgcctgt aaggatctga cgatcctggc agaatcggag aaggccggcg
     5341 tattcctggc aatggcggaa gagggcagaa agatttttgt agcgggccat ccggaatatg
     5401 accgttatac actgaacaac gaatatcata gagatctgga caaaggactg cccattcaga
     5461 tcccctataa ttattacccg gacgatgacc cggagcagag acctctgtta cagtggagat
     5521 cccattgtaa caacctttac agcaactggc tcaattacta tgtataccag acaacaccat
     5581 atgacttaac ggaaattcga taatttcgat agaaacagga cgatggtcat ggagaatgga
     5641 aaaaagaaag gaagcagaag atgaatcaga cactgacaag aaaacaattt gatattttat
     5701 cgatactggc agaggaaaaa ggtactttat cccagagaca gctgggagaa aagagcggac
     5761 actctctggg aacggtcaac cgtgtcatgc aggaactgac cgaattacag tatgtgacag
     5821 agggagagat cacaggtgcc ggaatctccg cactggagcc ttaccgtgca aagcgcgcca
     5881 tctttattgc agccggattc ggcagccggc tggtgcccat tacctttaac accccgaaac
     5941 cactggtacg tgtgcatgga cagcgtatta ttgacggact gatcgatgcc tgtctggatg
     6001 ccgggattaa tgaaatctat attgtccggg ggtatctggc agagcagttt gatcagctgc
     6061 tgtacaaata tcccatgatc agattcctgg aaaatccggt ttacaatgaa gctaacaata
     6121 tttcctccgc catggtggcc agatacatgc tttccaatgc ctatgtcttc gaggcggatc
     6181 tgttgatctc caatccgaag atcattacca agtatcatta cacctctgat ttcctggcta
     6241 ttaaaaagga cagaaccgat gactggtgct tcatagtgaa ggacggcgtc atcgtagagg
     6301 agaaggtcgg cggcctggat tgctggcaga tggtgggaat ctcttactgg aacgaagagg
     6361 atggacacaa gctgtcagac gatatcaaga tgacctatga gcaacccggc ggaaaagagc
     6421 gctactggga gcaggtgcct ctggtattct gcaagaagca ttataaggta gaagtacgtg
     6481 aatgccggga aaatgacatt atcgagatcg atactttccg ggaactgaaa gccatcgaca
     6541 agacgtatga tgtttaaaga aaaataagta ttgctatttt gtgcgaatgg tattatgata
     6601 tgttcatatt taacacaaag tgtctgcgaa tggaacgcag acattttgtg tacttatttt
     6661 agccgaaaac ctatgaaaac cgttcaaaga attaaaacat atcatacatt aatgaacgat
     6721 tgggaaaaga acgtgaacaa aaaaaggagg aatcccataa tggagcagtt gatacaaaaa
     6781 cccgtcaaaa gaaatatttt attgaatccc ggaccgtcta ccaccactga taccgtaaaa
     6841 tacgcacagg tcgtgccgga catctgtccc agagagaaag aattcggcgg attgatgaag
     6901 gggctcagag aagaccttgt aaaaatcgtg catggagact tggaaaagta taccagcgtc
     6961 ctgttctgtg gatccggcac catcaatatt gatgcctgca tcagctccct gctgccggag
     7021 ggcggcaaag tactggtggt ggataacgga gcctacagca ccagagctgt ggaagtatgc
     7081 cagtattacg gacttcctta tattgacctg aaattcccgg tggacgaact gccagactta
     7141 gaaaaagtgg agcagaccct gaaggagaac ccggacattg ccctggtaca taccactcat
     7201 aacgagaccg gcaccggcat tctcaatcct atccgtgaga tcggagccct tgctcataaa
     7261 tatcatgctg tttttaccgt ggataccacc tctacttacg ccatgcgtcc tattcatata
     7321 gaagaagaca atattgactt ctgtatggct tccgcccaga agggtctcat ggccatgacg
     7381 ggactttctt ttgtcgtggg aagccgggca gtcttagagg catcgaagga ctatccgaag
     7441 cgctcttatt actgtaacct gtatcagcag tatgactttt tccagagaac cggggagatg
     7501 cattttactc cgccggtaca gacaatctac gctacgatcc aggcattgaa ggaatactgg
     7561 gcagagggag aggaagccaa gtgggcgaga catacgagag tctttgaggc cattcatgaa
     7621 ggattggatg cactgggatt ccacgatgtg atccgcagag aatggcagtc gggactggtt
     7681 gtgtctgtga agtatcccga tgatccaaac tggaattttg aaaaagtaca tgattattgc
     7741 tatgagcgtg gatttaccat ttatcccggc aaaatctcta ctaccaatac cttccgcctg
     7801 tgtgcgctgg gtgcgattga cagaccggat atcgaagcct ttttcaaagt gttcagagaa
     7861 gcactggaag tcaataaaat tcagattccg gcaaaatata aggaggcata ggatcaaatg
     7921 aagcgagtat atacctgttt ctgtacggat gtcattcatg aggggcaccg caatatcatc
     7981 agagaagccg gaaaatacgg cgaactgacc atcggcgtct taagtgatgc ggcaatgatc
     8041 cgttttaacc gttttcccac catcagcttc gaggagcgta tgcagttagt aaaagatatc
     8101 ccggaagtca gcaatgtggt cgtgcaggat gatgtaatgt atgataaggt cattgaagaa
     8161 ttgcgccctg actacgtgat ccatggggat aactggcagg agggtgcttt gaaggctatc
     8221 cgtgacaacg tggaaggact gctgaagaca tacggcggtg agatcataga tgtcccttat
     8281 acatataatg aagaagtaaa aagaattgat acgaggatca aggaaaagct ggccatgccg
     8341 gaataccgca gaaagcgact gcgccagctc attgagatcc gccccattgt caaagcactg
     8401 gaggttcaca gcggcctgac cggactgatc gcagagaaga ccatcgtgga gcatgatggt
     8461 gagctggacc agttcgacgc catgtggatc agctctctgt gcgattccac ggcgaagggc
     8521 aagccggata tcgagctggt ggacatgact tcccgtttcc gtaccattga tgacgtgacg
     8581 gaagtgacca cgaagcccat tatttttgac ggagataccg gcggactgac ggagcatttc
     8641 gtatacacag tgagaacact ggaaaaaatg ggtgtttccg ccatcattat tgaggacaag
     8701 accggactta agaaaaattc cctcttcgga aacgatgtgg tgcagaccca ggacagcatc
     8761 gagaacttca gtgccaagat cgcagccggc aaaaaggcac agctgacaga tgatttcatg
     8821 atcattgcca gaatcgaaag cctgatcctg gagcgaggca tggaagatgc actgaaccgt
     8881 gccagagctt tcgtagcagc gggagcggat ggcatcatga tccacagccg taagaaatcc
     8941 ccggatgaga ttctggaatt ctgtgataaa ttccgggcag aggattcgaa gacacctatc
     9001 gtggtggtgc cgtcttcctt taacagcatt acggaaaacg aactggccgc ccacggcgtc
     9061 aatatcgtga tctatgcgaa ccagctgacc agaagtgcct tccccgccat gcagcagacc
     9121 gccgaggaca tcctgcggta tcacagggca caggaagtgg acagcagact gatgccgatc
     9181 aaagatatta taactttaat agacgaaatt taactgacag gggcaggaaa ggaatactta
     9241 catgaaagca gaaaactttg cgaaaatttt aaatgcggac ttttataccg gggtgccgga
     9301 ttcccagctg aaagccctct gcaattacct gatgcacacc tatgggatcg atccgaagca
     9361 tcatgtgatc gcggcgaacg aaggcaactg cacggcactg gccgccggct atcatctggc
     9421 taccggcaaa gtcccggtag tatacatgca gaacagcgga gaaggcaata tcatcaaccc
     9481 cgtggcatcc ctgctgaacg ataaagtcta tgccatcccc atgatcttcg tcatcggctg
     9541 gcgcggtgag ccggggatcc acgacgaacc ccagcatatc taccagggtg aagtgaccct
     9601 gaaattgctg gaggatatgg gaatccgcta ttttgtcatc ggaaaagaca caacggaaga
     9661 ggaagtaagt gctgccatgc aggagttccg caaggagctg gatcgtggtg aggatgtagc
     9721 gttcgtgatc cggaagggtg ccctcagtta tgaggaaaag gtggagtatc gcaatgacaa
     9781 tcccatgatc cgggaagaga tcatccgtca catcgtacag gcctccggcg aggatcctat
     9841 cgtcagcacc acaggcaaag ccagcagaga actgttcgag atcagagaac acaacggaca
     9901 gagccacaag tatgatttcc tgaccgtagg ttccatggga cacagcagca gtatcgccct
     9961 gggtgtggca gtacagaagc ccgggacaaa ggtatggtgc gtggacggtg acggcgcagt
    10021 gctgatgcac atgggttcca tggcggtact gggcagtacg gcacctgaaa atatggtaca
    10081 catcgtcatc aataacggtg cccatgagac cgtgggcggt atgcctaccg tggcagccga
    10141 tatggatctt gtctccattg caaaagcctg tggctaccct catgccgtat ccgttgccac
    10201 ctacgaggaa ctggacaggg aactgaccgc tgcaaaagag agagatgccc tgacctttat
    10261 cgaagtgaaa tgcggcattg gcgccagaga agacctcggc agacctacta ccacagcact
    10321 ggaaaacaag cagaacttta tggagtatct gaaaaatctg ggttaaattt tgttgacagt
    10381 agtaaaaaag aaatgtaata taacagatat agaaaagtca tgtcaaataa tgctctagga
    10441 tgtaagtttc tcagaagatg agtagaacaa attcctgggg cattttttac tagtgaaagt
    10501 cagattgtgt actggcacga ttaaaagggg caaaaatggg aaacttacaa tatcggaagg
    10561 tcacgagaga agtggaaaat acttctttta catgtggtgt tccatccata gatgaatatg
    10621 tgaaaaattc atattatcct accatagtac agcaggcata tggttatagc attagtggca
    10681 gggggattgt tcttggatat tatatgataa tgtttcggga gatagaatta gacgattttc
    10741 ctccggagat agcagactat gatccggaaa ttaaggaccg gaaagtatcg gctgttcata
    10801 ttcgttatat agcaatagac gaaaaatatc aacataacag gattggtacg gctacgattc
    10861 aggcaataat aaaaaatgtg gaattattgg ctgccaactg gccggtcagg gtaattacga
    10921 tagatgccag agaaaatctg gttatgtggt atgaaaaact cggctttcgc aaaatggtat
    10981 ataatacacc ggggcaggat ggtgttactg tttcaatgtt tttagattgc atgcagaaaa
    11041 aagaagaatt tacggaatac atagacagta tatatgaata ggagggaaaa tatatgaatg
    11101 aggaaagagt tggagacatt gtactccatg gcaacgatgc tattcgactt gcgtatatgc
    11161 tttatcgccc aacggaagag gaattactat ataacaggag cgttctggaa cggattgata
    11221 acaatattac aatcacggaa agagataatg ggtatgatgc ggaaattaag gatctggact
    11281 taagcttttt agaagaaaag ccgcaatgca gtctgggtaa tatagaggtt acatggacgg
    11341 tcaatattca aaaagaagaa tttttctact caaacagtag taatgaaaat cgggcgcaaa
    11401 tggtggtgga tacacagaaa acatcggtat atgaaaacac aacaagtgat ggaatgatct
    11461 tggtggcagc ataggaggaa acatggaaag tgcatttcag tttagtaatc cctcgctgac
    11521 aaaaatggaa ttttgcatta atgaagattt tgaaaacgga cgggataaag aagtaaagat
    11581 ggcggtgaac atgggaatcc agattgacag aagcaaggtt gataacagcg caaaagtcag
    11641 ccttactttg gaaattggga aaaagggggc atcagatccg ttctatgtgt gtgcaacaga
    11701 aatggcggaa ttcagatggc cggatagtct gaatatggga atgatagaaa aattattgaa
    11761 tcaaaatgcc ccgtcattgt tgctgggata tttgagaccc attatttcac agattacggc
    11821 agcatcgccg tataatgcct ataatttacc gtttatagat ttttcagaga aataagatga
    11881 tgtatcgtgc actttttgca cagttatttt tcataaaaga atgaaaattt atctatataa
    11941 aaggctgcct gtatggtaaa atacatacca tacaagcagc ttttcatata ttacaaaagc
    12001 agaaagaatg agggagtaca tatggaaatt aacattggta ttatcgcaat tattgtattg
    12061 gccattgcca tcctggccat cctggcatct ggttatgtga aggcatcacc gaacaaagcc
    12121 tacatcatct ccggtattaa gagagagcca aaggtcctga tcggacgtgc cggcatcaag
    12181 attccttttc tggagaagaa ggatgagctg atcctgaagc agatcagtat cgacattaag
    12241 accaacggct atatccctac taaggatttc atcggtgtgg atatcgacgc cgtggctaag
    12301 gtcagagtcc tgacccagag agatgtcacc gtaaatgcga agggtgaagt cgtggcaggt
    12361 gcggatccga acaagaggat caccaccgag atggctaacg cagccatgaa gaacttcctg
    12421 aacatgaacg aagatcagat ccgcgatgca ctgaccgatt ccttacaggg taacatgcgt
    12481 gagatcatcg gtacccagtg cctgaaggaa ctgtgtaatg accgtaagac tttcggtgat
    12541 gaggtacagg caaaggcgca gaaggatatg aatgccctgg gtatctggat cgagtcctgt
    12601 aacatccaga agatcgagga tgagaacaac ctgatcactg ctttgggaca ggataacatg
    12661 tcccagatcc agaagga
//
LOCUS       DAWDHD010000066        12666 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_320_length_12666_cov_9.143525, whole genome shotgun sequence.
ACCESSION   DAWDHD010000066 DAWDHD010000000
VERSION     DAWDHD010000066.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 12666)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 12666)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12666
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_320_length_12666_cov_9.143525"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    10633..10732
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2810 a         3534 c         2694 g         3528 t
ORIGIN      
        1 gtcgcatcaa ttctgccgca cagtcttacg aatgcgagca ttcgacgcct gtcagcagaa
       61 ttgcgctact ctgaatagtt tacacgttga agcagcttcc acccacaaat tccctgcaca
      121 gagaattatt gggaaggctt tcactggtca cacccaggaa atactccagg aacttaagca
      181 gtctcttggc ttcgtaatat tccggttctc ccttatcgat ctggaacaat aacggctctg
      241 cgaactgctt taacactgcc agttcataga tcaggacgga attaaacatg gcatccgcat
      301 cctcctggaa cgggaaaata ttctgttcct caccccgtcg tacagaagcc cacatatcga
      361 tggtgcgtct cgcatccgca cctctggtcc tcgcatctct gaccatacgg cggagaagtc
      421 ttccgtccgt agtcgggatc cggttatgct catcaatatt gatattggtc aatgcactga
      481 tatagatctt gtatttactc tcctcgggaa gtgcataaga tgtcttctca ttcagaccgt
      541 ggataccttc gatcaccaga atatcatccg gtcccagttg tttgaaattg cccttatatt
      601 cccgctttcc ggtcttgaag ttaaaggaag gcagttccac acgttcaccg gctaacaatc
      661 tgcacatgtc gtcattgaac tgctttacat caatcgcttc cagacattcg aaattgtaat
      721 caccgttctc atcccttggc gtgaattctc tgttcacgaa atagtcatcc agtgcaatgg
      781 gatgcggtgt tttccccagg gtcttcaact gaatggataa tctgtgggaa aaactggtct
      841 tgccggaaga agagggtccg gcgatcatga caaatttcac accgcccctg tctacgatgc
      901 tcttggcaat ctcgccgatc ttacgctcct gctgcgcttc ctgtaccagg atcagttcac
      961 tcagcgaccc ccggcagatc tgatcattca gatcacctac ggtctcaata ccgatctcct
     1021 ttccccattc ctcagattct ttcaacgtcc ggaataacag ctttctctct tgaaacggct
     1081 taacatgtgt aggatctttt ttatccggca gcagcagcat aaatccatcc tcatacgcta
     1141 tgacatcgaa ccactttaca tagccggcat tgggcagcat gtagccgtaa taataatcgt
     1201 aataaccgtc catttcataa atatttacga aagagcctct gcggtaacgg aacagctttt
     1261 ccttatcctt catcccctct ttccgaaaca gttccatggc attatccagg gaataggatc
     1321 tcttcagaaa aggtgtcttt gcctccacca gctcccgcat ccggttacgg atctttgcag
     1381 cattttcttc cgttgcattg atcttccctt ttggagagat gtaggagccg ttgccgatgg
     1441 caaattccac acgacagctc tgggccgcct ctctgccgta gacgtcaaat accgctttca
     1501 aaaacagcat tgtggcagaa cgcacataag tcttgttgcc cacatcatcc ttcaaagtaa
     1561 aaaaggtaac ctcgcaatcc cttgtcagtt tcttgaacaa ttctctgatc ttgccgtctc
     1621 tggctgccaa tgcgatcaga ttctcatact cctgctgata atcgctggcc acatcctcgt
     1681 aagtggctcc ctgaggatat tgtctctctt ccccgtttat cttcactgtg atcatttttc
     1741 tcaccctgtc cttctgtaat ctgcttttac aggtcttatt tttcaaacgc aatccggttc
     1801 cttgccatta tcataccaat ccagtgcacg ggtaatcagg gtgatctcgt ctgcgatctc
     1861 ctgtctgcgg catatggttc tgggattgtc cgcatctgcc ttttgctcca tgcgtcccac
     1921 gatctgcctt cgctcggtca gcatccattc cagatactcc cgaagtatcc ggtctctgcg
     1981 ggataaaagc agcgggccga acttttccgc cagtccggga tccagacctt ccgtcttcca
     2041 ggagtccccg accttctgcc gtaccgcatc gtccgtcatc gcacccagcc agcgcacttt
     2101 catcataaaa taatattttc cttcttcgta gatgatattc tcatccatca gaagatatcc
     2161 ctcttccatc agaaatgtcc gaaaatgcgg gatctccgac tggggctgta agatcagttc
     2221 ccgaaaccct ttcgcctgtt ctcttccttc ggtaagaatc ttctccatga gaggcccacc
     2281 catgccggca cagatcatcc cgttcgcctc tccggtcttc aggccgtgca gaccgtcgga
     2341 aagtctggtc tctatcctgt cctgcagacc atattcccgg atatgctctc tggcacgctc
     2401 caggggccct gaccgcacat ccatggcgat cacccgtggg gagagcccct tctgcacgag
     2461 atagaccgat acaaaaccat ggtcacaccc cacatctgtt atcacagtgc cgggagtgac
     2521 catgtctgtt aaagcctgca ttcgattgga taatataact tccgccatta atccagataa
     2581 tcctttagct tgcggctgcg gctgggatga cgcagtttgc gcaatgcctt tgcttcgatc
     2641 tgacggatac gttcacgggt aacgttgaat tccttaccta cttcctccag agtacgggcc
     2701 cgtccgtcat ccagaccgaa acggagtctc agtactttct gttctctctc tgtcagagta
     2761 cccagcactt ccaccagctg ttccttcagc agggtaaatg ctgcagcatc ggaaggaacc
     2821 ggtacattat catcctgaat gaagtcgcct aaatggctgt cttcctcttc accgatagga
     2881 gtctccaggg atacgggctc ctggctgatc tttaagatct cacgcactct gtctaccggc
     2941 atgttcatct cttccgcgat ctcttcgggg gtcggttctc ttcccagctc ctgcagcagc
     3001 tgtctgctca cgcggatcag tttgttgatg gtctctacca tatgcaccgg gatacggatg
     3061 gttctcgcct gatcggcgat cgctctggtg atggcctgac ggatccacca ggtcgcatag
     3121 gtggagaact tatatccttt acgataatcg aatttctcta cagctttgat cagacccagg
     3181 ttaccttcct ggatcaggtc taagaacagc attccacggc ctacatatct cttggcaata
     3241 ctgactacca gacgtaagtt ggcctctgcc agacgctttt tcgcttcctg gtcgccctct
     3301 tccattctct gggccagttc gatctcttcc tcagcactta acaggggcac cttgccgatc
     3361 tcctttaagt acatacggac aggatcctca atgctgacgc cttccggtac ggacaggtcg
     3421 atgttctcca catccacttc gtcctcatcg gtcagaatga tctcttcgtc atccacatca
     3481 tcgtcatcgg taatacgcag cacatctacg ttgttctggt ccagatactc cagaaccttc
     3541 tccatctgtt cctcttccag aggcatgtcc gcaaagaagt cactgatttc ctgatactcc
     3601 agcacattct ttttcttttt cgccatattc aggagctctt ttatcttctc cgcaaatttt
     3661 gcctgtgtgt tttcatccat tttcgtggtt ccatcctttc attgccatta tttatagcca
     3721 tatttcgtat tttttataac tgtcccgagc cgcaaaccct attattccag agaaatacgg
     3781 gctctttgta attcttccag tgcttttttg ccttccaccg cttttttgac cgcgttgaca
     3841 tctgtaccca gctgcgccat atagtactgg tagctgttgc gtttgactga caccagaatg
     3901 tcatggaaag ctctttctct ctcctgcttc gtggacaatt cggggagcgt tgtatggaac
     3961 agctccgctg cctgcctctg ctcctcttca tcctcaaaca tgctgatgat ccccgccggc
     4021 tgaaactgtc cgttgtccag ctctgcaaac atcttctccg ccaccttgcg atacaattcc
     4081 tccgtgaaat cctgcagaga aatgtactta cgtacaatcg tatacagaga aggatcatct
     4141 ccgatccagg tgatcagcag tctctgggac ttccgtccgc tctccgaagg atcgctcttg
     4201 ttctgcactc cgctgcgggg acggggaatc ggtctggcga ttcccgtctg ggaagcatat
     4261 cctatgacca ttttgcgcag atcatcggga cggatcatat atttttcggc aatggcctgc
     4321 aaataattct cccgctccag atcttccgaa aattcgcaca atttctgtgc gatctcccgg
     4381 tggaacttcg tccgctgttc cggatcactc tgatcgtatt gttcactcag gatacggatc
     4441 tcgaagtaga aactgttctc cgcctgatcg attctctcct gaaaagcctc tttccccaga
     4501 ttcttgataa attcatccgg gtccttataa ggacgcatat tgatgacttt accggtaagc
     4561 ccggcctccc tgagaatacc gatgccccgg agtgccgcct tggttccggc accgtcgctg
     4621 tcgtaggcca gcaggatgtt ttccgcataa cggtgtaaaa ggttagcctg ttccaccgta
     4681 aatgccgttc ccagggatgc caccgcctgg gtaaatcccg cctgatgcat ggctatgaca
     4741 tccatgtagc cctcgcacag gataatattg cccgtccggg cagtccttgc gaaattcagt
     4801 ccataaagat tccttctctt atcgaagatc atggtctccg gtgaattcag gtatttcggt
     4861 tccccgtctc ccatgactct gccgccgaag ccgatgaccc ggtggttgat atcctgtatg
     4921 gggaacatga cacgattcca gaactggctt cgcagtcccc gctgttcaga aaacattgcc
     4981 agcccggagt ctttgatctc ttcgtccgta aatccctttt tccgcaggta ctgtaccacc
     5041 tgctcaccgt ttttcccggc ataacccagt ccgaaatgat gcatggtctc atcggtcagc
     5101 ttccgccccg tcagataccg cattcccact tcgccacggg gatcccgcag catataataa
     5161 aaatattttg ccgcttcctt attgacttca agcagccgca tacgtttgcc ggcttttttc
     5221 ttctgttccg gtgtctcctc cacttcggga agctttaccc cggcgcggtc cgccaggatc
     5281 ttaatggcct ccgggaaggt atcgttttca tatttcataa gaaacgtata tacatttccg
     5341 ctggcatggc atccgaagca ataatacatc tgtttgttcg aagacaccga aaaggaaggt
     5401 gttttttcgt tatgaaaagg acagcagccc caatagttgc ttcccttttt tttcaatgag
     5461 acatagcccg atatcacatc cacgatgtca tttctggctc tgacttcttc taccagttct
     5521 tcgggataat acacgttttt aactcctatt ttatcttgta cctatctctg ccaggatttc
     5581 ggtatgtaaa tgtcctcgta taccgctatg gcaaaccgat ccgtcatcgc tcccacatag
     5641 tcgcagacga ccttctctct cggttctccc acggaaagaa gattgagata ttcttccggc
     5701 agatcatcca gatgcttcag gaaatgctgg tacaatgtct ccatcagcat ctctgctttc
     5761 ccctcttcac tcttcgctac cgggttcttg taaactctct caaacatgaa ctgtcgtatc
     5821 ttcgccatgg cttccgccac cggctccgac atcaggatgt ctcccttgtc catgctgttg
     5881 gtcacgatgt catgaatgaa atgatccagt ctctcggtgg gactgctgcc gatcacctca
     5941 cagatgtcct tcggcacatc cgcttccgtg aggattcctc cgcggatggc gtcatccata
     6001 tcgtgatgaa tataggcaat tttatcggaa agtctgacga tcttgccctc cagggtgtgg
     6061 ggctttccaa tggtctggtg gtttcttata ccgtctcgca cctcgtaggt caaattcagc
     6121 cccttgccat ccttctccag aatgtccacg gtgcggacgc tctgttcggc atgttcgaag
     6181 ccaaaagggc atactctgtt cagcgcacgc tctccggcat gtccgaaagg cgtgtgtccc
     6241 agatcgtgtc ccagcgcaat ggcttccacc agatcctcgt tcagctttaa tgccttggct
     6301 atggttctgg ccgtctggga tacctccagc gtatgtgtca gtctggtacg gtaatggtca
     6361 ccctcaggtg tcagaaatac ctgggtctta tcctttagcc ttcgaaaggc cttgcaatgg
     6421 ataatacggt ctctgtctct ctgaaacacc ggccgaatat cacattgttc ttccggtcgc
     6481 agacgtcctt tggagttcac actcaaggat gcgtacggac ttaaataatc cttttcccac
     6541 tgttccaagt tttctctgat cgtcatagtt cctcctgccc ctacttctaa tattctgtgc
     6601 ctgtcttaaa aatccttttc tttcttttga aaaaaatagt cattttttct gatttaaatt
     6661 atctgcacac agaatagatg aattccgcat tcttatccat ggcctcactg gcctttttga
     6721 agggtgacat ctgaaaaaca tgccacattc cggggaatcg gtcgatatgg gcagcgaccc
     6781 cggctttgat cagattttta tatagccgca gagaatcgtc caacaggatt tcattctctc
     6841 ccacctgaat atagacgggc gggaaaccat gaaagtttcc aaacagcgga gaaatataag
     6901 gatccgtcag atctctgtcc ggggcatagg ccctggtcat acggtcgatg tactctttgt
     6961 ccagtaccgg atccagttcc gccttggtct ggaaagtctt gccctcgccg gtaagatccg
     7021 tccagggaga catcagcacg attcctctgg gaagcaagcg ctcctgctct tttaatttca
     7081 acaccagtga aagcgccaga ttacctccgg ccgaatctcc ggtaatgatc acatctcttg
     7141 ctccgtagcc caaaagcatc agatagttcc aggctttcat ggcatcctcc gcagctgccg
     7201 gatacggatg ctccggtgcc agtctgtaat caaagcacag cacatccatg gaagtacccg
     7261 ccgccagctt cgatgtcagg gttctggcat agatactgct gccggtggaa tatccgcctc
     7321 cgtggcaatg caggatcacg tactttttca tatgagcccg gttcacgctg acccactccg
     7381 catccatgcc atcgatgtcc acgggacggt actggagctc tttactgttg ccgagcagct
     7441 cgccaatgtg atcctgggat tgtctgtgct tctccagatc cggattctcc acaagaccat
     7501 gggccctgcg gatgagctgc atcagattct ggttgttctt ctcactgttt tttcctacca
     7561 gtgccataag ttcctcctgt tcacctagtt ttctcgtaac tattcagcgc ctcattcgca
     7621 aaatcgcatc tccgatgctt tcctgaacaa caagttcact tgttgtttgc tcatctgagg
     7681 ctgcttcgcc aaagaaatat tgtccagccg tcttacgaat gcgagcattc gacgaggctt
     7741 tgccaatatt tcatggctct gaatagtgat caaaagtgca atcttctgcg cactgcattg
     7801 cgcagtctcc ttcgggataa aagggtggcg atggtgatat cgacgatcac gcagatcgtc
     7861 agcacgatag ccgaccatcc cagtgcgatc tcctgtctca ggaaccggtc gatcagaagt
     7921 tccagtccca cgcacaaaac agcgattccc gagatcaggt acagggctcc tgtcaggaag
     7981 gacatgggga gcttgcggat acagaacgtc accgcttcca ccactgccgt caccagcaat
     8041 acgatcggca gtcccagtcc atagaaccag ctgttatgtc ccgtcagata agtcagcaga
     8101 tatagataca cgatcactgc cactccatcc agcagaatcg acagataaat gggctgcctc
     8161 gtataaataa ccacagggat cataataacc cacaatacca ggactacgcc aatcaccgcc
     8221 agagaccaca gactgctgcg gaacacaaaa gcgtttaaca gcccacaggt caccgccgta
     8281 gccagtacaa taaccgtcaa cagtactccc agatcttttc tttttaccac ttccacctgt
     8341 cccttttcct ttggaaaagg ggatggctgc ttctccggtt ccggtgcctt gggattatac
     8401 accggtgtgt cgcacagagg gcacttcttt gccgaagcat ccagttccac accacagttc
     8461 acacaatatg acataagcat ttcctttcta accggaggat ctaccggtta ctctcgatct
     8521 tcacatgaat accgtcctgt accagcttgc ggaaaaacag tcgttccaca tccgtttccg
     8581 caatactgct ggcaaaattg atggtcagaa tatcttcaaa gctggcaatg gcacagttgg
     8641 ttctcgtaga gaacggctgt cccatataga tatcaaaacg ttcgatatag ggcttcatgg
     8701 tctccggcac tttcaaagct cccatgttgg tcagtcccgc cgaagagttt ttgtcctgga
     8761 tcttcgtata gaactggcgg accaccagat ccttgataaa cagcggtacc acacggataa
     8821 acgggttcct ctgggtctct gcgttggtgg tgatatctcc ccgcaacagc ttttcattca
     8881 gataataccg catgtaattg tgcacctggg tcacgatctc ttcaaaagtg tattctccca
     8941 gtcttgggtc gatccccgga tagatcatgg tgataaaatt ccgcagagtc ttcgatggga
     9001 aaaagcgccg cagattcacc ggcatggcaa tctttaccgg acgtaatttt aaatgccact
     9061 cactctcctg cttctgcatc agtgcataga gcagaaccgc attgagatat tccgtaatgg
     9121 tggcattata gctttttgcc accttcatga cctctgccac cgacataata ccgtctatga
     9181 tgttcagggt ataaaagggc tccttggttc cccggacacg gtaagtcttc tcccccggtc
     9241 tgggcgggca caccttggca ttggcatatt tcatgtaggc atcttccagt tctaccggat
     9301 ccggtgtctc taaaatatcc agcacaaaac cgccgttctc gatctccgta tgccccttca
     9361 ggcgcagata agtggccgtc agagtctgca atacgcacat gcctccggta ccgtctccca
     9421 gactgtgatg ggcctccagg gcgatccggt tccggtaata atagattctt accagatacc
     9481 ggttattggc cttgaaatac atgggctgac agggattttt cacgtcctcc tgcacaaatg
     9541 ggccggggcg gtcattgggc tccaggtatc tccagaagac acccttgcgg atagccgcct
     9601 tatacgtcgg aaaacgaggc attgtcagat tcaccgcccg ctgcagcatc tcgggattga
     9661 tctcctcctt taaaagaacg gacaaacgat aaacggaaga aaaatctctt ctctgtaagg
     9721 tcgggtatac gatggccgac agatccagtt tgtaccagtc cttgtgtact ttcgatgtcc
     9781 tgtctgtctt cgttttgtct cttctattaa ttgtaattgc cacggttttc gcccctttcc
     9841 cggttttgta ttatattttt cagaaacgac aaaggcggca acctttcggt caccgccatg
     9901 tgatgcagaa agagggactt gaaccctcat ggtatcacta ccacacggac ctgaaccgtg
     9961 cgcgtctgcc aattccgcca tttctgcgaa caaatgtata ttacagtatt taattaaaaa
    10021 tgtcaacacc tttttcaaat taccttaagt tacttgaaaa agtgatgcag aaagagggac
    10081 ttgaaccctc atggtatcac taccacacgg acctgaaccg tgcgcgtctg ccaattccgc
    10141 catttctgcg aacaaatgta tattacaaca accggctcca aatgtcaaca ctatttttcg
    10201 aattatttaa actgtatgca aaataattac gatttctgcg taaaaaatgc ggctgaccgg
    10261 cagccgcatt tttgcaatta ttttacattt acatgtagga actgaacaga ttgcctgcat
    10321 tgtaagtgcc ggtatatcct gctgttccgg tgtacagact gcttctggtg gaagcatggt
    10381 ctgctgcaaa attgatcatg gaactctgtg cagatacacg ataaccatag gaaccgttcc
    10441 cctggaacag gttcttcaca gtattcatat ccgctttcat aaaggtatcc ttgtccagag
    10501 acagagtgcc gtcatttttc atagtgatgc cgatcttcgc caactgctta ctgttggcaa
    10561 tggtggtatt ccccagcgat tccgtgcggt tgttcactgt ggtatcgctc acatcatcca
    10621 cagcagccat cannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngattccgt
    10741 gcggttgttc actgtggtat cgctcacatc atccacagca gccatcacgc tgttgtaatt
    10801 cttcacaaag ctgttcaccg cattgtagat ggcactggta tcatagccct tggtcgtggt
    10861 ctctatacca ttctcatcct tggtggtgat atccttttgt gcgaacagat catttttacc
    10921 ggtagccagt aatgtatctg cggattcctt caaagcatca gtggcggtct ctaccttggc
    10981 cagtgctttc ttcgcggtct ccttcgtggt gtctttcttc gttatgtcgg aagctgcggt
    11041 tttggatgcg ttgttctcct tgccgtaata agccttcatc agcttggcat agctaccgtt
    11101 cttaatactt gcataatctg ccagccagtt gccgcttagc gcattggatg cagaggaact
    11161 cagtccggaa aacagaaatg aataattggt ctttgcctga atattaatac tcatcacagt
    11221 cacctccgtg tcaactgttt tgaatcggcc tttcagccat tagtcctttt atatcagtta
    11281 cagtatacta cttatttcgg atttttccaa ggtttgttaa ctgtttttta aaaaaatccc
    11341 attaattgag tattttttcc agcatggtca cccggttgaa gggaaaggag aaataatagc
    11401 cgtccgcaaa atcctccgtc atgacgatca cttcatttgc cagctgcaca ccggcctgct
    11461 ctccctcttc tctggtcatg tccgcccggt atcttgccag tacttcgtcc gtcacgtcga
    11521 tgcctgccat ctcgttcttc ataaaggtgg catttttaag actcacgaac ggcatgatcc
    11581 cgcagagaat ccttgctccg gtctcctctt tgatccgacg cactctgtcg gcactctcct
    11641 gtgtggaaat gggctgtgtc agaaaaaagg tagctcccgc cgccattttc ttcttcactc
    11701 tgccgatctc cacctcaaga ttacgcctgc cctgattgat ggctccgccg tagctggccg
    11761 gagcctgcgt gaactgctcc tcgttcatat ccgccagaat ctgcatcagt cccacagaat
    11821 caaagttgaa cacactcttc accgtggtcc gcaccatgga aggaatgggg tctcccgtga
    11881 tcaccaggaa attctgaata ccattgatat aggctcccag cagctgggaa cgcatggcaa
    11941 tggcattttt atcccggcaa cagatatggg gcatgacaca cataccggtc tctcttgcca
    12001 ccttctccgc catgagaatg gaatctgctc tggtacgtcc cgaaggagaa tccgggaaag
    12061 tcagcacatc cactccgctc ttctgtagca gatgtgccgc atccatcagt ttttcgtcat
    12121 cgattcccat gggaggtgcc agttccacgg cgatcagctt tcggttcctg ccctccgcct
    12181 ctttggtatg ataaaaagaa cggtcttcct tcggtgcctg ctgtttctcc ggttctgtgt
    12241 ctttttttgc ctgtgggtac tgggtcagat ccagctttcc ggcaagctcc gcgatatatt
    12301 ccggtgtcgt accgcagcag ccgcctgcca tatccactcc aagggcagtc atctgctgca
    12361 ttctgtccac aaaatagtct ttgttatccc cggtgaagag catacggccg gtgaccatct
    12421 gaggataccc agcattggga agggctgtga gaagttttcc ttccggtttt tccagtatct
    12481 gcaggatccg gtgcatatgg gacggaccca caccgcagtt aaagcccacg gcatcgatct
    12541 cctttgcctc acccgctttc tgtaaaagct ttctggcact taatcctgca ttgctgtagc
    12601 cgaactggtt tacagagaat tgtaccgtaa taaatgcctg ttccccgatc attttgatcg
    12661 caggca
//
LOCUS       DAWDHD010000067        12415 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_324_length_12415_cov_12.976456, whole genome shotgun sequence.
ACCESSION   DAWDHD010000067 DAWDHD010000000
VERSION     DAWDHD010000067.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 12415)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 12415)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12415
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_324_length_12415_cov_12.976456"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2904 a         3429 c         2712 g         3370 t
ORIGIN      
        1 tcatgatacc ggaatacata caatccgcct cttccgtacg gatcttgata ttgtcgtagg
       61 tatactgcaa gatctcggat accatctcac aggcggagga tgcataaggc tccacataag
      121 acaatgtggc attctcaatg gtctccgtac cctgtctgtg gtggtccagt actacgacgg
      181 atttgcagaa gcgaagcaga tccgggcact ctgtgatgga gggcttattg acatccacca
      241 ccaccagaac cgtattgctg cctgccatat caatggcctg ctggctggtg atgatcatat
      301 cctcgtcata ttccggatta ttcttaaaca gctccaccag cggctgtaca gaggtcgtag
      361 cctcgttcaa tacaatgtga gcctttctgc ccaatgtctg ggcgatccgg taaataccca
      421 ccgcagaacc gaagctgtcc acatccggca tccggtgtcc cataacgata acacggtctt
      481 ttccagtgat aatctcccgc agggcatgag cttttactct tgccttgaca cgggtattct
      541 tctctacctg ctgactcttg ccgccgtagt aggtgatgct ctccggggtc ttgatcacgg
      601 cctgatcccc gccacgtccc agtgccagat caatggcggt acgggcgaat tcatagttct
      661 gtgcataagt caggccatcc agaccaatac cgatactgat ggtcactgcc atttcattgc
      721 cgatgtttac agtcttgacc tcttccagca gatcgaaacg gttctcctgc agctgtgcta
      781 ccgctttttt ccgcatgatt accagatact tgtccttttc cagcttcttg ctgataccgt
      841 ccagagatgc aatgtacttg ttcaccttac ggtcgatcag tgcgatcagc agggatctgc
      901 gaacttcctc aacgctctcc agcgcttcct catagttgtc cagatagatc atgcctacgc
      961 tcagactctg gtcatccact tcctgcagtg caatatgcag tgccgtctca tcaaagagat
     1021 agacggcaat cagataaccg ttatagcctt ccgcatcgat catatcgctg tgttccgcca
     1081 tctccttcag agaaatcttg cggaatttgg ccacatactc acagccctcg tattccagtt
     1141 catactgtgc ctcgtccaca ccagattcgt ccggaagcct gtcccgggta atggtaggaa
     1201 acagggaagt gatggattta ctgtagccct tgggctgatg caccacattc tcaaaggcaa
     1261 tgttggtcca gatcaccttg ccggaatcgt ccagaagggc atatggcaat tccagatccc
     1321 gcagcagttt tcgctggatc tgaccatatt ccgtggcaaa gctgatcagc tcgttcatga
     1381 tgatcggctt gttgtagaaa tacagcgtca gggtaattgc aaaataaaaa atcgtaaatc
     1441 cgaaggtaag cagtcctgca cggaaatcca acatgaatac cgccgcatcg atagccagca
     1501 gtaacaggcc cagatatagg gtaaactgga tatatgtacg gatcctgcct ttcagtctga
     1561 ttcgtttttt catgaaagaa cacctctcaa tatttgcgtt cgacagcttc tattataaca
     1621 aataataacc cgctccgcca ccatattatt ttgacgaata gacaatattc ctctgtcaaa
     1681 tttgtgcttt ttgtcagctt ttccggacgg agagccgttc tcttacttac acagttccca
     1741 gaaagccact gcgctggcgg ccgccacgtt cagagaatcc acaccatggg ccatgggaat
     1801 gcacaccgta tcgtcacagt ccgcaatggt atccgctgcc agtccttccc cttccgttcc
     1861 cagaataatg gcaagctttt cgacattatg cagtttctca tcatccggcc gcaagctgtc
     1921 ttcttttaaa gccatggcta cggtatgata ccccatcttc cgcagctgtt ccatggtatg
     1981 ctccggccag tcctttgtct ccaggatcgt ccacggcacc tggaatacgg ttcccatgct
     2041 gactcttgct gcccgacggt acaacgggtc actgcagcct cccgtcaaca gtactgcatc
     2101 gattcccaac gctgccgcag aacggaaaat tgcgcctaca cttgtgggat taccaacatc
     2161 atccacaaca gcaatgcgcc cggcattacc gcactagtcg tccatcttcg gcacggctct
     2221 tcagcgcatg gcacagagca ttcctctcgc cagtttaaag ccggaaatct ccgacagcac
     2281 ttcattggat gccacatata cgggaatttc cccagacacc tctgcaatcc gccccaggat
     2341 ttccctgccc ttctccgaat ccgcttcccg ctgttccatc agaagtgaca gcggctcata
     2401 tcccgctgcc agtgcccgca gaatcacatt ggggctctcc gcaatgaaca gcccccgctc
     2461 tggctcataa taatgaaaaa gttgtacctc cctgcgttca ttatagatct ccagttccgg
     2521 tgtatgcaga tcagtaattg gtattaatct atgcttatct gtactcatgc ttttgcgata
     2581 ccttttctcc aataattttc tctttttatt ataatggagc atccgacccc cgtcaaacgc
     2641 aactgttacc ctgtactcgg ctcatggtat acacaaaaag accagccaca aagggctggt
     2701 ctctcatgat tgtcttagtc tgaattattc agctacgtaa ggcataagtg ctacgtgacg
     2761 tgctctcttg atagcaacgg tcagtgctct ctgatgcttt gcacaagtac cggtaatacg
     2821 acgaggaagg atcttacctc tctcagatac atacttcttc agcttagctg tatccttgta
     2881 atcgattacg ttatctttac cacagaatac gcaaactttc tttcttctgc gcataccgcc
     2941 tttttttctc atcggagaat cggtcttctc agtcttatta aaagccatta ttattacctc
     3001 ctatcggaat ctgtcagggc agaggcgggt gtcagctccg cttgtcactg aaacttagtt
     3061 aaagggtaat tcttcatcga tgccgtcagg gatgttcata aagccatcac ctgctccgga
     3121 aggagcaggg gcattattac cgccaccaaa gccagcgttc tggtatccac cgtcattatt
     3181 gccggcagat gcgttcttac tctctgcgaa ctctacgttc tcagccacta catcagtagt
     3241 atacacacgc tgtccatcct tattggtata gcttccggtc tggatacgtc cttccacaac
     3301 gaactttgtt cccttgtggc cgtatctctc aataaactca ccggttctgc caaacgctac
     3361 acagttaata aaatcagcat ccggctcacc gtcacgctta aatcttctat ctactgctat
     3421 ggaaaaacga gcaatcgctg ttgcggtagt tccctgagaa tatcttacct caggatccct
     3481 tgttaaacgt cccataagaa ttactttatt catatttcgc ttctcgcttt ctgtttatgc
     3541 ctcgtcctgt ctaacgcata agtatctgat cacgttgtcc atgatacgaa cacggctctc
     3601 gatctctgca ggaacagtgc tctcagcttc aaatctgatg aagtagtaga aagcttcttt
     3661 catcttctga acttcgtaag caagcttctt cttgccccac tcgtccactt ctgtgatgtt
     3721 gccgccgaat ctctcaacca gtgccttgca cttgtcaaca acggctgctc tctcatcatc
     3781 ctcgatctta gcgctaacaa caacggctaa ttcgtacttg ttcatgttag ttacctcctt
     3841 ttggtctctg gcccatattt tatatatgag caaggaataa agtgtctcac aaaggaacat
     3901 gataccatat tcctgtccac ttttcaagct ttttcaacga tttctttcac atttttttct
     3961 aacagttttc ttgcgatcat gatctggtgt ccacagccca gacatttcag ccggaaatct
     4021 gctcccaccc ggagtacttc ccattctctg ctgccgcagg gatgctgttt tttcagtttc
     4081 aatacatctc ccacattaat atccataaca ttttcctctt cttttcctgc ctgtatcgcc
     4141 actcctccgc aaacatgtcg cgacagaggc ctgtgattct gtattataaa tggttcgcca
     4201 taaagtcctt cactaccccg aaagcctccc gcagcaggtt attcggtacg gtaacgggat
     4261 aagttcccgc ggacagtccc tctacatggg catagccctg ttttctcgca atagccaacg
     4321 cccggaacat atgaaaattg ttggtaataa ccacaacact gtcctctgat ttgtccagca
     4381 gttctccgct gaagatcaga ttctgcgtgg tattcgtgga agcatcctcc gtgaggatcc
     4441 gctccggatc gatcccggcg gtttccagat atccttgcat tccctcggcc tcgctgatgg
     4501 gttcattgta tccctgtcca ccggacacga tgactttggt ctccggattg gcattcagat
     4561 attctatggc tttatccagc cgtttctgta gcacataact ggggccggaa gtcttccact
     4621 gggctcccag tatgatcaca taatccgcac cttcctgcgc cgtggcatgg aactgactga
     4681 cgatcatccc ttctataata agaagcaata ccatccctgc catcacaaga ctccggaatg
     4741 taatatgcag ccatcggggg atccagtaat atacggttct gtgggtcagc acccatgccc
     4801 atgcggcaca aaaagcacca atcacacccc agaccaggaa aaagtagctg ccgaaggcaa
     4861 caaaaagacc gatccccaca cagtacagca gacacagcac cgccagaaca tataacgccc
     4921 ttgctccgta atgtttttct cccacacctc tgcctctggg atccagatag atcacttttt
     4981 tacttttttc cctcggaatc aagtggttcc ttttcatact gcctccattc tttcctacga
     5041 tctattgtag catatctcct acggcagtct caataccaca tattttaggg ttttcttaat
     5101 tttttcgaaa attttcttta ccagacactg attgacttgt cactcaccta acactatgat
     5161 gtaattaaca tactacttct gaaaaaagaa acggatggaa accttatgca taccacatta
     5221 tccaaaaaag atttttcccg atatctgccc ttcctcctgc tggtgatgac cgttttccgc
     5281 gtcctcgccg gactgaggat tccttatatg atcctggcaa atcagcgcta tgatgaccgg
     5341 ctgctgttcg agaatgctta cgatctgctg agcggcgtgt ggcttggcag ctacgattct
     5401 tatacactgg caaaaggaat cggctatccg ctgtttctgg tactggcgaa aaagctgtgc
     5461 cttccctact ctgttctgct gtcgctgttg caggcggccg gtgcatggct gtttgtccgc
     5521 gcagtgtctg tgagatggca gaatccctat gggcaggcaa ttctctatct gctcctgttg
     5581 ttttccccca tcagcctcac gctgttggtc acgcagcggc tgtaccgcat ggcaatcgtt
     5641 ccgggaatgg tcctggtggt attttccagc atgatcgggc tgactctgcg caaaaagctc
     5701 cccttgaaaa aacagctgcc ctgggcattc ctgaccggac tgactcttgc ctttttctgg
     5761 cagatccggg aggattccgt ctggatcctg ccatttatcg ccgttatgac ggtatggaac
     5821 gtgggttatg taattctggt cctgcataag aaattaaaca caaaggcact tctgcttcac
     5881 tgcctgacca tgttagtgcc actccttctc ctgttcggcg ccaacaccgg tgtctccgtc
     5941 gtcaaccgga tccactacgg tgttttcctg aacaacgacc gcacggaggg aaattttgca
     6001 gagctgctgt ccctgttgta tcatctggac agcaatacga gaaccaatcc cgacatctgg
     6061 atctcccggg atactattgt cagagcagaa gcagccagtc ccactctgca ccagattcag
     6121 ccactattgg acagttatac cgaagactgg gccacaaggg atggtgagat tcccggtgat
     6181 cacttcagct gggtactgcg ggatgcggta caggactcgg gaattgcccc aaatgctgtc
     6241 agtacacaga ccttttatgg caacgtcctc tccgaactgc gggcagccgt agccagtggc
     6301 gagctgaccg aaaagacgga cggtgccctc tatttttcct cccaatcccg tggcgtactg
     6361 ccggaagaaa ttcccggtat cctcagtgat actttgcaga atatctggaa aattgcagga
     6421 tacaccgatt gtgctttgag cagttccgcc aaaagtgccg gccggctgtc ggatatccgt
     6481 cgtatggaat cctttgcctc ctgcctgacc gtgtacccca cactgtcaca gtttcaggct
     6541 gccgattatg attccattga ggatgagacc ttatatgatt tcaatgagat ctacagttcc
     6601 ggtatggtat ctcttctgaa taagagcaac gctatctatc agttgttggg acagcccatg
     6661 ttcctgctgg cactgcttgc cctttgtgtg ctgaccgtcc gcgtgatcca tggacttttc
     6721 catggggaca caaaagatct cgaactgtgg atcctgacct gtgggattct gctcagtgcc
     6781 gttctgctgc gtttcggctg tggcttgttc accgcatggt tttccgaaga catgcaaaag
     6841 ttcatcaaca gcttctactc ctgtggcgtc tacattctgc tgcagatgtt caaatatctg
     6901 gcggttatta ctactgccgc cagcctgcaa ccgatacgga aacaatattt tcaaaatttt
     6961 cgtaattctc ataaggaaaa ggaggaataa cttatggaaa atttgaacaa acagattcag
     7021 gaattgcaaa agatcattga tacctacggc aatatcgtat ttttcggtgg tgccggtgtc
     7081 tccaccgaga gtggaatccc ggatttccga agcgtggacg gactgtatca tcaacagtac
     7141 gattacccgc cggagacgat tttaagtcac accttttatc gttcccacac ggaggaattc
     7201 ttccgttttt accggaataa gatgcttttc cccaatgcac agcccaatgc cgctcataaa
     7261 aaactggcag aactggaagc tgccggaaaa gtccgggccg tgatcaccca gaatatcgac
     7321 ggactgcatc aggctgccgg aagcaggacc gtactggagc tccacggctc cgtgcttcgc
     7381 aattactgtg aaaaatgcca tcagttctac ggtctggatg ctatcttaaa cagcaccggt
     7441 attccccgct gtgaaaaatg cggtggtatc atcaagccgg atgtggttct gtacgaagag
     7501 ggactggatg agaagacgtt aaatgccgcc gtcagataca tccacgaagc agatgtcctg
     7561 atcatcggcg gcacttctct ggctgtctat cccgcagccg ggttaattga ttattttcag
     7621 ggagataagc tggtggtcat caacaaaagt gcaaccccca gagacagcca cgcagatctc
     7681 ctgattcagg gaccgatcgg ggaagtattt cactcgatcc agaatccgta agtgatatcc
     7741 tgttctccgc tgatatgata agcatcttcc acatcagcgc cccagctgtt gataccaccc
     7801 acgcccctca ctgctcccag gatagatacc accgtcctgc gggcaggagg tagttcttcg
     7861 tggtgcatgg cattttccag ttcctgtgcc gtataaggca ggcaggaaaa tgcgaagtgt
     7921 tttccctcct ccgcggttat ttttaaacgg ctgcttcttt ctccacggtc tgtattatcc
     7981 agggaagtct tcctgacgat ctccagccat ttggtctgca tatgcatccc gcagtcctga
     8041 ggcaccatat aaggtgtcac cggcaggccc tgtacttcat acacaccgtg gatccctcct
     8101 gccatacggt ccggataggt ctctccggac aaaccttcgt aagtatagcc ttccgccgct
     8161 gtgggcatta aaaatcttaa gcccaatacc ggaagctccg gcagtccctg ttttccgaga
     8221 taatccacct gtacatggat ccggccgtcc gattccacca gatagcttac cgtgacttcc
     8281 gttaccggta cagtgggtgt ctcgtaagta taggagattt cgaaccggtc acaggtttca
     8341 tcaccgttgt atttattatt ttccggtgcc ggaggcatgc tgatctcctg ctcatccatc
     8401 cgggccgtta cctttacaca gcgcacaaaa cgatccgccg cagaccagac cgcactgcgg
     8461 aaaacaaagc cattacctct gtcgttgtcc gtggctgccc gccagaaagc cggtctggga
     8521 gcccggtaca gccactcttt tccatcctta tataaagatt ccatgccgcc cattcctaca
     8581 gagaaaatat aggcaaaatc ctgtccctgt attccgatgg tcacatcacc gatcacgatt
     8641 ctgaatgcca taatagtatc tcacctcctg cattgccggc ttttcgctgc gatttgcgaa
     8701 aagaattccg ttgccggaaa attcatagtc gctgggacgg tcatcaaagt ctccgccata
     8761 gcgcaggact ctccgtcctg tgacttcatc ttccacccaa agcgcctggt caatataatc
     8821 ccagataaat ccgccctgat acatttcaaa gcggtccaat aattccatat aggacttcat
     8881 tccgcccaga gaattgccca tgtcatgcat atattcgcat aacagatagg gtttcttggg
     8941 gtcatgttcc agataatcta taatttcctg cgggtatgcg tacatacggc tctccacatc
     9001 cgagatccgg tcttcataag ctctgttgtg gaagacacct tcgtaatgta ccagccgttg
     9061 atcgtccttt tccttgaaaa actgctgcat ggcagcaata tcatctccgg cataggattc
     9121 atttcccaga gaccagaaca ggatcgaagg atgattctta aagcattcaa aattggttct
     9181 ggcacggtcc actaccgctt ccttccactc cggcagggaa ccgggcacat tccaggaagg
     9241 ttccacagct cccatcttct gccaggagcc gtgactctcc agattcgtct ccgccatcac
     9301 atagattccg gcttcatcac acagtccgta ccattccttg cggtccggat aatgacaggt
     9361 tctgaccgca ttgatatggt tacgtttgaa gcattcgatg tcccatttca tgtcttccag
     9421 ggaaatacac cgtccggaag ctgcattcca ctcatggcgg ttcacaccac agatcaccag
     9481 ccgctcacca ttcagcttca ttaccttatc ctgaggatcc agcgcaaaat cacggaagcc
     9541 tattttatat ggcaccactt ccgtcacttc cccctcactg tctgtcagag tgatatccaa
     9601 ctgatataag tacgagttct catggctcca ggggattact ttttctgcca atgtttcccg
     9661 aaggtgcaac gtctgctccg cctgtgactg caaaatctgt gtctgctcca acaggcattt
     9721 cccgttggca tccgacaggc atacacgcat tttgcccgga cgctgtccct caccctcagc
     9781 agatattttg attgctgcat ctaacgtgcc ttttccatcc tctacggaaa aatatggctt
     9841 cgcccaaagg tcttccacgt gccactgcgg cttggcatat aattccacac tccggaagat
     9901 tccgaaaaag cggaagaaat cctgatcctc cagaaaagca gccgtactgc gcttatgcac
     9961 ttccaccgcc agcagattgt ccttttcctt aatataaggt gtcagatcga attcagaggg
    10021 agtaaagctg tcctcggcat atcccacgaa atgcccgttc agccagacat acatcgcctg
    10081 ttccacacct tcgaaacaga tgatcaccct ttttccccgc aggccttccg ccaggtgaaa
    10141 acgcttgcga taacttccta caggattata ctctgcttcg ctgaaagcac cttctgccaa
    10201 tccttttccc agcgtgtaag ccgggcggcg gtaaattttc ccctcccagg gatacatggt
    10261 attgatataa tgaattttgt cgtaaccgga taactcaata tgccccggta cacggatcgt
    10321 ttcgaattct gtatctccgg cattgtcccg gtagaaatcc accggacggc ttgcggcatt
    10381 cacagaatag gcaaactccc agtttccgtc cagagactgc accagagagc ttttaccttc
    10441 tgctgcttcc tccacatccc cataaatggg atgatcactg tgggctggca gctgaccgac
    10501 ccggaacacc ttcggatcgt ccagccatct gatatctgct tccattttgg tcctccttat
    10561 ttatcccttt tctattataa taattgtgaa ttttcttcca tgcaacaaat atttatcttt
    10621 tcgaaaaaac agattccatg atacactgcc cggaaatcaa gatttttgtg gcagcatatg
    10681 tgaaaatatg ttaatctgta aaagtatttg ccttatatcc acaaatgata ggagattctg
    10741 tttatgttat ccacaattga agcagtcaac agtgttgtta acaattttat ctggggtgtc
    10801 cccgccatga tctgcatcat cggtgtcggt ctgtatttaa gtatccggac gcgcttcctg
    10861 cagatccgaa agttccccta ttccatgaag gtgacactgg gacgtatgat gaagaaaaaa
    10921 gaggcctccg atggagccct gacacctttc caggcggtat gtaccgctct cgccgctacg
    10981 gtaggcacgg gtaacgtagc cggtgtggca ggtgctatcg ctatcggcgg ccccggagcg
    11041 gtattctgga tgtgggtatc tgcgctgctc ggcatgtgca ccaagtttgc cgaagtaaca
    11101 cttgctgtac acttccgcga gaccaatgca cagggcgacc tggtaggcgg tcccatgtat
    11161 tatattaaga acggactgga taaaaaatgg cacttcctgg cgtatttgtt cgccgctttc
    11221 ggtgtgctgg ccgtattcgg taccggtaat gccacacagg tcaacacgat cactactgcc
    11281 attgattctg ctctgagcag ctttaacata ctgcctgcag atgctgtaaa gcttgtcaac
    11341 ctgatcatcg gcgtggcact ggcgatcatc attgccctga tcctgatcgg cggtatcaag
    11401 cggatcggca atgtcacctc caagctggtt cctttcatgg cgatcatgta tatcgtgctg
    11461 gcactgggtg tgatcttttt ccatattaag gctgtacctg ctgtattcgc ttctattatc
    11521 gaaggtgctt tccatcctgc ctctgtcacc ggcggtgtgg tcggaagctt cttcatgagc
    11581 atgaaaaagg gcgtatcccg cggtatcttc tccaacgagg ccggccttgg taccggatcc
    11641 atcgctcatg cctgtgccga tacgaagaag ccggtaaagc agggtttctt cggcatcttt
    11701 gaggtatttg tggacacgat catcatctgc accatgacgg ctctggtcat cctgtgcagc
    11761 ggtgtatccg taaattacgg tgaagctgcc ggtgcagagc tgaccatcag tggcttcacc
    11821 gctgtctacg gcagctgggt atcgatcttt accgctgtgg cgatgtgctg cttcgccttc
    11881 tccaccatca ttggctgggg actgtacggt accagatgta tcgaattcct ctttggttcc
    11941 cgttccaaca tgcctttcat ggtactgtat tcccttacag ctatcgtggg tgcgacaatg
    12001 aacctcggac tgatgtggag catcgccgag accttcaacg gactcatggt catccccaac
    12061 ctgatcgccg tgttcctgct gtccggcgtt gtagtgaaaa tgacaaagga atatttcgca
    12121 aaagagaacg gataatatct gaatgggatt tgcgttatga tcattgaata aatgatgtag
    12181 ttctttcgtt cgataatgac agtgaattga aagttagtga aataatcccg tagcccttgg
    12241 cagggtataa gaaacgcggc acgctcgcgc tctgtcgcag agcgtagcgg tacataagcg
    12301 gtcacaatac gaccgctaag taccgacgtc tgcttacggc actgcttatt cttataccct
    12361 gccggggcta ctggtttttg aaagctttcc aaaacgcaga actcccggag tatgc
//
LOCUS       DAWDHD010000068        12334 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_330_length_12334_cov_10.318430, whole genome shotgun sequence.
ACCESSION   DAWDHD010000068 DAWDHD010000000
VERSION     DAWDHD010000068.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 12334)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 12334)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12334
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_330_length_12334_cov_10.318430"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3432 a         2648 c         3346 g         2908 t
ORIGIN      
        1 aaagccggtt atgaatccgt tgtttttgga gaaacgtgtc tatcagggaa gctcgggtag
       61 gatatatccc cacacggtga tcgagaagat cagcgatgag aaagtggata aggagtatac
      121 agcgcttttt ttggagaacg attatctgaa agtaatgatg ctgccggagc tgggaggcag
      181 gatccagaga gcttatgaca agacaaatgg ctacgatttt atatattaca atcatgtcat
      241 caaaccggcg ttagtgggac tggcgggacc atggatttcc ggcggtattg agttcaactg
      301 gccgcagcat cacagaccca gtacctttga tcaggtggaa tatacctatg cggaaaatga
      361 ggacgggagc gccacagtct ggatgggaga gattgagaac atgttccgga ccgaaggagt
      421 gctgggcgtg accctgtatc ccgacaaggc atacatcgaa ttgtctgcaa agctatataa
      481 ccggacgaaa atgccccaga ccttcctgtg gtgggcgaac cctgcagtag cggtaaatga
      541 tgacaccatc tccgtatttc cggaggatgt gacggcggtc tatgaccacg gcaagcggga
      601 tgttatcagc ttcccctatg cggagggaac ttattataag cataaatacg accatgtgaa
      661 tattgcccag tataaaaata ttccggtgcc cacctcctat atggcatatc gttccgatta
      721 caattttata ggggagtacg attacggaaa acaggcagga cttctgcatg tggcagatca
      781 tcatattgct cccgggaaaa agcagtggac ctggggatgc ggagaattcg gcaaagcatg
      841 ggaccttgca ctgaccgacg aggacggtcc gtatatcgaa ctgatgacag gctgttttac
      901 ggataatcag ccggatttca catggatcca gccccaggag accaagaatt tcaaacagta
      961 ttttatgccc tataaaaata tcggctatgt gaaaaatgct acgatcgatg cggcggtaaa
     1021 tgcggagtat gatgagacaa aggggcagct tacggtatcg gcctatacca cttccgtgca
     1081 aaaaggggca cagatactgc tgacattgtc gggagaaaac ggcagacagg aaaaggtact
     1141 gtatgaggaa acctcagatc tgtcaccgga ggaaacttat gaagtaaaga tcgaccgtga
     1201 aaaattgcaa cagataccga catttgcaga gggagagcag aatggaacag aggtactctg
     1261 cggtctgcgg gtatgtgttt gtgccgcaga tgggagagaa ctggtctctt accattttcc
     1321 gaaaaaaatt gaagcggaag tgccggaacc ggcgaaagca gcgctacttc ccgaagactg
     1381 tgaaaccacg gaggatctgt tcctctacgg tctgcatgtg gaacaatacc ggcatgccac
     1441 ttatcatccc gaggattatt atctggaggg gctgcgcagg gatcctaccg atatccgtct
     1501 gaacaatgtc tatggaagat gtctgcttaa aaagtgtgat tttgccgggg cagaaaagta
     1561 ttttcggaag gcagtggaga aatccattcg ttccaatcca aacccttacg attatgagcc
     1621 gtactataat ctgggacttt ccctgaaata tcaagggaaa gaaaaagaag cctatgatgt
     1681 cttttacaag gccatctggg gtggcagctt ccaagcaccc ggattctatg aactggcctg
     1741 tctggatgca aaagcgggaa gattcgcaga ggctctggag catgtaaatg aatctattct
     1801 cagacagtac cagtgtatga aggcccgtgc attgaaggaa aatattttaa gaaaactggg
     1861 aagagtgaag gaagcagaag aattacataa ggagagtctg aagatcgatc cgctctatga
     1921 cagacagccg gaaaaaataa atcataacac tctgctggaa ctgatgatcg atctctatga
     1981 ggcaggagac tacacgcagg gaattgccct tgccgaaaaa tgggtggaac agaaatccgc
     2041 caagggggag aacatttatc cgctggtata ttattatatt gcttacggat atggcaacct
     2101 gcccggtgcg gaagctgcca gaccggcaga acattatgcg gcattgggag aaaaagctgc
     2161 agccgacggc tgcttccctc acagaacgga agattatatc gtgctgaagc gtctggcagc
     2221 agaatacgac cttggcatgg cgtattatta tctgggctgc ctgctctatg acaagagagt
     2281 gtatggagaa gccgtggcgg actatgaaaa ggcggcgctt aaatacccgg agttccctac
     2341 ggttcacaga aacctggcgc tggcatatta caatgtgaaa aagcagccgc agagagcata
     2401 tgaggaattg acaaaggcct ttgcgctgga tgagacagat gcaagagttt atctggaact
     2461 ggatcagcta aagaaacgtc tgaatatctc catgaaggaa cgactgagcg atatggataa
     2521 acattttgtg ctggtgaaaa gcagggatga tctgtatctg gaatatgtga cactgatgaa
     2581 cactctggga gaaaacgcaa aggcgcttgt cctgatcgaa gcaaggcagt tccaccagtg
     2641 ggaaggcggc gaaggcaagg tggcagcaca atacctgacc gccctgcatc agctggcaaa
     2701 agcggctgtg gtaaaagggg actatgcaga ggcaaagtca ctccttctgc gggcagttgg
     2761 ggaataccct cacaatctgg gagaaggaaa actggagtcg gcacaggaaa acaatctgta
     2821 ctatctgctg gggctggtgc aggaaaaact gggagagaca gaggatgcgt tagactgcct
     2881 gacaaaagcc tgtcacggag agtcggagcc ggtgggcatg atgtattaca acgatcagcc
     2941 gcccgaaatg atctattatc aggggctggc gtaccgaaag cttggtgacg aagaacaggc
     3001 agtacagaga ttctgcaaac tggtagacta tgggaaagag catataggcg atgagataaa
     3061 gatagattat tttgcggtat ctctgccgga tctgctgatc tttgaggaaa acctgaatga
     3121 gcggaatcgg aagcattgcc tgtttatgat gagcctggga ctgcgggggc tgggacagac
     3181 ggacgaagca aaagcctgcg cggaagaact gctttccatg gacaatgcac accagggaat
     3241 ccgggtacat gatctgtaat cacgcgtaaa aagaaatttt tgatagattt tactggaaaa
     3301 ggacattcaa atgaaaaagg aaatctatag ctataacagc gaatacttaa cctgcaacgg
     3361 cagtccaatc ctgccaatca tgggagagtt ccatttctcc cgttacccgg aaaaggagtg
     3421 gagatctgcc ctggagaata tgaaactggg cggagtggag atcacagcca cttatgtctt
     3481 ctggatccat catgaggaag cggaacgaga atgggatttt tccgaaagga ggaacctgaa
     3541 agcctttctt aactgctgtc gggaagtggg aatgaaagta tggctccgca tcggtccgtg
     3601 ggcccatgga gagtgcagaa acggaggctt cccggactgg ctggtggaga aggaaagacg
     3661 gggagaactg actttgcgga cggacgatcc acagtatctg agatatgtcg atgtcttttt
     3721 taccaaaatc gccgagcagg cggacgggta tatgcataag gacggcggtc cggtcatcgg
     3781 tatacagatc gagaatgaat acggacatgc cggcggtccc tccgacaggg aagagggtat
     3841 ggcacacatg cgcacgctac gtgccatggc ggagaaaaag ggactggaag ccccgtatgt
     3901 gtcggcaacc ggctggggtg gtgcctatgt gccgggaagt ttcctgccgg tgctgggcgg
     3961 gtatgtggat gccccgtggg caaatcatac ccatgaactt gcggcaagcg agaatttcct
     4021 gtttcagcct tttcacgacg atgccaatat cgcatcggat ttcgccgaag gacagagtgg
     4081 ttttaccttt gatacgtcaa aattccctta cctgacggcg gagctgggtg ggggactgca
     4141 ggtgacggca cacagacgca cctaccctta cccggaggat atcgaggcac agacgatctg
     4201 catgttggga gcaggggcaa atctgatcgg gtattatatg taccacggcg gcgtgaatcc
     4261 tgacggaaaa tataccaccc tgcaggagtc gaaggcgacc ggctatgcca atgaccttcc
     4321 ggtgaaaaac tatgattttc agacgtgcct gcgggaaaac ggtctgccgt cagaaagcta
     4381 ttatcgactg cgcaagcacc atttttttat aaaaaacaca caagaactgc tggcaccggc
     4441 aaaggtgtac ctgccggata atatctccga gcctgcgagt gcggaggaca tggagacgct
     4501 gcgggcagcc ttccgttata ataaaacggc ggactgcggg ttcttattta tcaataatca
     4561 ccagagaaaa cggaaaatga cggagaaaca gatcacaccg gaaaagccat tacagtttac
     4621 ggtgacagat gttgaaggaa tacagaggca gattattttc gaccggattc atgtgcggac
     4681 ggatgcgatt ctggtgctgc cttacaatct gccggtggtg atacggggag agcagttcag
     4741 acttcggaaa accaatgcct cttatctggg atgctttggc ggaacttatt atttctatac
     4801 ggatgagaag ccggaggata tttatttcga gtggagtgat ggcaatgatc atgcggaagc
     4861 ggtaaggatc cttaccatac acgatgcgga gcatttttgt tatgcgcagg aaggggcaga
     4921 tgaaaagggc aaggtgagtc tgctgccgga tctgcacttt gcggaagccg gtaaggtgcg
     4981 gatcgcggat gccggacagg cagtggaaag catctggaac gtatatggtc aaacagaacc
     5041 gaacgtctat gaattaacct tggaatatga gtatcacccg gcggatgcac tgtctggcga
     5101 tgtctggctg gaactggatt tcggtgggga ctgcgcacgc ttgtatcagg acggaaaact
     5161 gattgacgac tggttctcca atggtgaact atggcgtgtg gctctgaaac gctacagctg
     5221 cccgacacaa ctgacgctgg aactcgatcc gttcaaaatg gatgtctatt acgatctgcc
     5281 accgaagcgg gagatgcgtc tggcaggggc aagactgctg cggttaagtt gaccggaacc
     5341 cggtttggca ggactcactt tggaaacaga aggatagaaa agaggataca gatatgaaag
     5401 acatgacaca gggaaaaccg ttaaagctga tcatcggctt cgccataccg atgctgatcg
     5461 gcaatatctt tcagcaggta tataatgtgg cggatacagt gatcgtagga agattcttgg
     5521 gagagagcgc gctggcgggc gtgggaagca cgggaaccct gacctatttt ctgctggcac
     5581 tggtcagcgg catgtgtaac ggcgcaggac tggtggttgc gcagtgtttc ggctgcggga
     5641 ataaagagcg tctggcgaag acggtgacag cactgatctg ggtggcggga gtgctgacct
     5701 gcatcatgtc actgatcggt attttcggtg cggaattttt cctgcggctt ttaagcgtgc
     5761 cggaaaatgt tatcggctat tctgctacgt atctgcatat catttatact tttgttttcg
     5821 gcagtgtggt ctacaacggc tgtgcggcga tcttaaacag tgtgggggat tcgaagactc
     5881 cgttgcgggc ggtgatcgct tcctcggtag tgaacatcgt gtttgacctg tttttcatca
     5941 ttgtgtgtaa gatgggagta gcgggagcag cttatgcgac gattctggca cagtgtactt
     6001 cggcggtgat ctgttttgtg aaagtaagac aagtgagaga agagatcgga ttatctctga
     6061 tgaaatggaa accggaagtc cattatattc agctggtggt gaagaccgga ttcccggcgg
     6121 cattccagtc ctgcatgatc gcactgggcg gaatgagcgt ccagagactg gtgaattcct
     6181 tcggatcctc taccatggcg gcctatgtgg cggcgaaccg tgtggacagc gtggcgatcc
     6241 aggtcattgt ctccatcgga acggcattgt cggtatttac cgggcagaat attgcgaaga
     6301 atcaatatga ccggatccgg gaagccctct ataagacgct ggcggtcatg gtgggagcca
     6361 gcgtctgtat tgcgacactg gtgctgatct tccgggtaca gctgatgagc ctgttcctgg
     6421 atgccggcga atcggcagaa gccatcggca tcggctgcac ctatctggcc attatcggcg
     6481 tggcttatgt gatcgcgggg gtgatgcaga gctaccagaa tgtgatccgg ggagccggag
     6541 acgtcaatac ctgtatggta gcgggactga cggagctgtc cggcaggatc gtgttcgcgt
     6601 atctattgtc cggaaccctg ggcgtcacgg gaatctggat cgccactcct ctgtcctgga
     6661 gctgtggctg tgtgattccg gtgatccggt attattccgg caaatggaag cacaagaggc
     6721 tggcctgatg accggagtgg ccggaacaca gaggggctat tccggccgca tccatccggc
     6781 aaattccaga atggactggt tctcagcctt tttctcgctc tgctgcatct gcttgcggta
     6841 ttctctggga gacatctgca taagtttgat aaagtagcgg ttgaaactgg acacggaatg
     6901 gaaccccacc atttcggaaa tgtccagaat agagtgttcc gtactgcgta acagaataca
     6961 ggattttaaa atccgggtgt tgttgacgta gtctaacggt gaagttccca taatatcatg
     7021 gaaaacccgg cggaagtgcg tcgggctcca gtggcagaga tcggccagat attcgatcga
     7081 gaactgttgc atataatttt tctcaatata ttccagagcg ggtgagatca ccagagagtt
     7141 ttcggcgatc tcgccttctt ttttacgttc ctgtaaggcc agtatttcaa gagagtccgg
     7201 tttggccgtg tcgggattga caccgccgag actttcgatc cggtacaatt caatataaag
     7261 ggagcataac aggcttctgg cactgatctg atagcaggga ttctgctctt ccagctcgtg
     7321 gatgatatga gataccagat agttgatgtg gggaaaagcc tctttgctga aaatataacg
     7381 aaatgctggg aaaccgtccg tggacaggtc gtaatttttc caggaggcgg ggaggagatt
     7441 gcggaacaat tccttcggat ccaggaaaag ataagaccag tggcttttcg tgcctggtgc
     7501 actgtaagtg gtatgtggaa cattgcgggg aagaacggtg acatctccgg catggaaagt
     7561 ctgtttttct ccgttgatct ccagcgtacc gctgtcggaa tgacagatcc caatctccag
     7621 acagttatga aaatgaaggc ttccgctggg aatatcggaa atcttccaat aatcaccgga
     7681 cagaagcagt accgggaagt gtgttggcag ataataactt cggtattctg tgacagctcg
     7741 tttggatttc gccatagtgt gcctcctgtt ccagagtttt ctgaatagct ttgtgcatcc
     7801 tgtatgatga tgcaacggaa agatatgttg cagtagaaat aatgtctata cattatatat
     7861 aacaattaaa atggataaaa tcaaccatta tgttgcgaaa atgacttttt tagggttggc
     7921 aatgtttccg tggaagagat taaaaaaggc taaaaatacc gtgaaacagc gaatcctaca
     7981 gcaaaaacgc tccaaaattc acgcaaaatt tacgaaaatg caacgaaact tcaatactta
     8041 cgaaaaacga ccatgatcta taatgatcgt ttttgcatag ttttttataa tgattgatta
     8101 gaaaaatatg acaacatgta tgataatgta cttacaaagt tacaaaaagt gtacaaagtg
     8161 acgagaaaat atagctgaca cttgaggagg ttcgacatgg cgaagaagaa tgcagccaat
     8221 gtggtgggag tgaaaacgaa aaccacgtgg aaacaagctt ttaaaagaga ctggcaattg
     8281 tatctgctgt tactgcttcc actgattatg gtatttgtat tcagttacgg agcatacccg
     8341 ggattgcgga tggcctttat ggattataaa ccggcgaagg gatatgcagg cagcgaatgg
     8401 gtgggcatga agacattcgt taagatattc aaggatgccg atttcatgag agcacttcgc
     8461 aactccatag tgtttaacct ggcggatctg atcatcggtt tcccaatgcc gatcattctg
     8521 gcactgatcc tgaatgaatt aagatatccg agattcaaga aagtgtccca gacgatcctg
     8581 tatctgccgc acttcctttc ctgggccatc atcggaagcg tagccatgac aatgttccgt
     8641 ccgaactccg gtctggtgaa cattctgctg accaacctgg gagtgatctc cgaaggaatt
     8701 cctttcctga atgagaaatg gcactgggca attacatatc tgctgatcgg tgtatggcag
     8761 acgatgggat ggggcacgat cctctacctg gcagcaatca ccggtatcaa cggagaattg
     8821 tacgaagcag ccatgatcga tggagcgaac cgttggaaga gaatgtggca tatcacactt
     8881 cccggtatca agagcacagt agtaacactg ctgatcctga acctcggacg tgtcatggga
     8941 agtaacttag agcgtctgac cgcgttagaa aattcacagg tcaaggactt ccagtatcag
     9001 ctggctgtct acatttacaa caaaggtatt ggagccggaa aattcagtgc ggcgaccgca
     9061 gttggtctgt tccagtcatt gatcggattg atcctggttc tgttatctga taaattcgca
     9121 aagaagcttg gcgaagacgg attactgtag gaggtgagag agatgaagaa aaaagaagtt
     9181 aatattaatg aagaatctac ctccgcaggc ggagcacatg cggtcaacaa attccgcatc
     9241 agtgatttcg tcatgatggc agtgatcgtc atcctctgcg ccagctgtgt attaccgttc
     9301 ttacatgtag cagtaaaatc catatccggt aataccgcag taatgtccaa gagcgtttac
     9361 ttctggccca aagacatcac cttcgatgct tacaagaagg tatttgcaga taactccatg
     9421 acctactcca tgatcttctc ggtgatcgtt accgtgatct tcaccgctct gggaatgctg
     9481 gtatgtactt gtgccgctta tcctctttcc aagaagagac tgaagggcaa aaagttactg
     9541 actttcctgc tgatggtacc gatgtacttc agtgccggca tcattcccag ctacctgtta
     9601 tatcagaagc tgcatgtgct ggataccatg tgggtgttga tcttacctct gatctattcc
     9661 ccgtacaaca tgctgatcat gaagaactac ttccagagta cgattcccga cagcttagag
     9721 gaatccgctt tcctggatgg cgcaagtaac ttccagatcc tgagcaagat cgtattaccg
     9781 ctgtccaaac cgatcctggc aacgttgtct ctgttctacg ccgtaggccg ttggaactct
     9841 tacgcagaca acatgtacta caccaagagt gctaacctga agctgatcca gtacaagtta
     9901 tatcagttgg tagcatccgc agcagaagca cagacttcct ccctggcaga taccggcagt
     9961 gcgatccagt ccactcccga agtattacag gcagcaagta tcatgtttgt aacaattcct
    10021 atcattatta tttatccttt cctgcagaag tacttcgtac agggtaccat gatcggagca
    10081 gttaagggct aaaagttaaa aggaacaaaa caaccaagtt agttttcccc aaaaccgggg
    10141 gtacacataa aactatgtat aatttttaag gaggaaaatc ctctcctcgc cagggactcg
    10201 gatttttcct tcggaaaaac aaggaggatt gatttatgaa gaagaatact cttcacaagg
    10261 taatggcaac agcacttgtt ggtgctatgg caatgagcac tctgaccgga tgcggcagca
    10321 gtgcggacaa cacaaccgca gatgccggaa caaaggaatc tgtagcagag agttctgcag
    10381 caagcacaga tgcaggagca tccacagcag tagcaggtat tgacggatgg gaggctttcg
    10441 cagacaatgt tactctgcag atccccgtat acgatcgtgg ttcttccgga aacggatgct
    10501 ctgatgtaga gaacaactac tggaccaact gggtacagga gaacttcggt gataagtaca
    10561 acatcacggt agaatatgta ggaattaccc gtagcgacgt tatgactgat tacgctatgc
    10621 tggcagcaag ccagagcctt cctaccatct gtatggaata tgactatgat aagctggcaa
    10681 gctggcaggc agacggctat ctgcagacct acgatgtaga agagttcaag cagatcgctc
    10741 ctacttactg ggagaccatg gtagagcacg gcaacgatgc ttacaccaag ctggatgatg
    10801 aagattatct ggtactcggt tacagacctt acggtaacac cacttatacc tggtgcactt
    10861 tctatcgtca ggactggctg gaagaagctg gcttctctga gtatcccgtt ggcgacaaca
    10921 ccaagctgtt agagctgtat gcaaaactgg ttgagaacgg ccatcagtat cctttaagcg
    10981 gtaagaaagt aagcggcgca ggtattgacc agaactatgg ttacagagat tatcctcagg
    11041 atgagaccac atgggcaacc accggtgact accagatccc cgctctgtcc accgaggctc
    11101 agaagagatt cttaaaatgg gagaacgagc tgtacaacga tggttactac aatcctgagt
    11161 actatctgag agatgcttcc gaggcagagg cagacttcat caacggtgaa gcatttacct
    11221 ggaccggata tatatcctcc agcatgaacg tactgaactc cttctatgat gctaaccccg
    11281 atgccaagct ggcagtagca gtgactcctt ccacctggac acaggatgag acctggggat
    11341 cttccgcttc ttacagaccc ggaaccaact tcggtatgat gattggcttc gctaacgatg
    11401 ctaccgagga tgaagtaaag gcagctatga tgtaccttga gtggctgaac cagcccgaga
    11461 atctgttcac tatgcagtgg ggtatcgaag gcgtgaactt caattatgat gataacggcg
    11521 atcccgtagc agtagcagat cagaccggtc tggctgagca gcagggacac aacaacaacg
    11581 tagactactg gtgtgcagta accgcagtta agaccctcgg atctatcgag aaagatatcc
    11641 aggcagttac ccctcagggt attcctcagg acttctatga tcagatcctg gctaactaca
    11701 acggacaggt tgctatctat gaagcaggca aggctaactc cgactgtctg tttgcaaccg
    11761 ctatcgatgc tgttaccgag tacaaccaga cgctgtatga tctgtatact gagtaccgtg
    11821 acaagctgac catgtgccct aaggaccagt tcgattctct gtatgaagaa ctgagccaga
    11881 agtacctgga tgcaggttat caggaagtta tcgatgagag acaggctgct ctggatgcag
    11941 gtaacaccac cagacttcag taagtaaaaa gttttattac aggaagtaca tacagccccc
    12001 aggcatgaat ggcttggggg ctatattaaa aatgcaacta ttcagagcca tgtaaatatt
    12061 attcaatggg cgtcgaatgc ttgcattcgt aagaacattt gataatattt atagggcgaa
    12121 gttactttat gtccttttcc gaccgactgt tttacaataa aaacaactgt gggaaaagaa
    12181 tataaagagc caaaaggcag aaatggagga gctatgttaa aactggaata tgataagaaa
    12241 gaaatagagg gagtcattga caagatcgtc aaaaagacta tgaatatgga tctcacctgg
    12301 gactggccct gtggtgtggc atattacgga atca
//
LOCUS       DAWDHD010000069        12041 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_340_length_12041_cov_9.885366, whole genome shotgun sequence.
ACCESSION   DAWDHD010000069 DAWDHD010000000
VERSION     DAWDHD010000069.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 12041)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 12041)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12041
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_340_length_12041_cov_9.885366"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4729..4828
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3204 a         2682 c         3119 g         2936 t
ORIGIN      
        1 gagattattt tgtctcgatc catcgcaatt ccagtcctta tccgaaccag tacagtggag
       61 tggagagttt agtctacaac cgctatggag cggcagccag gatggcgtac aacatcaatg
      121 ccggactgga gcaggtgggg ttcgtaaatc tgggagtgaa tgaaagaccg aatctggtgg
      181 tgctcaacag cacaaatatg ccggctgtgc tgatagaagt gggatttatc aatacggatg
      241 cggacaacga actctttgac agccggtttg acgagatcgc ccgggctatt gccgacggca
      301 ttctggagag catctgattt tacctaattc ttacaaataa attaccggac tgtgatatta
      361 tgtgaatttc ccccatgtta gtatgattgc gtaatcaaga gaaagaaaaa caaaaacatg
      421 gaggacatta ttatgagagt aagacacagc aaattattag ctctgatcgg agcaggaata
      481 ttagcagtaa ccgcattggt tgcatgcggt aacggtgatg cagccacagc acagccttcc
      541 gcagtagaga gcagcgcagc agaaagcact gtggcagaga gcagcgcagc agcacctgct
      601 gagaccacca cagatctgtc cggcagcatt tccatggtag gaagtacttc tatggagaaa
      661 ttagccaatg cactgagcga agctttcatg gaagaacatc ctgatgtaac tgtaaccgct
      721 gaattcgtag gatccggtgc gggtattgaa gctgttacca acggaaccac agatatcggt
      781 aactcttcca gaggcttaaa ggatgaggaa aaggcagcag gcgttgtaga gaacgtagta
      841 gctatcgatg gtattgcagt atgtgtagac cctgctaacg aagtagcaga tctgaccaaa
      901 gagcagttga ccaacattta caatggcaca gttaccaact ggaaagaagt tggcggtgca
      961 gatgagccta tcatcgtgat cggtcgtgag gcaggttccg gtaccagagg cgcgttcgaa
     1021 gagctggttg atctggtaga cggctgcaaa tatgcaaacg aactggatag caccggtgca
     1081 gtaatcgcaa aagtggcatc tactcccgga gctatcggat acgcttccct ggatgcactg
     1141 gatgacagtg ttaaggcact ttccctggaa ggtgtagaag ctaccgcaga gaatatcaag
     1201 gccggtaact atttcctgag cagacccttc gtaatggcta ccaagggtga gatctccgag
     1261 cagaacgatc tggtacaggc atggttcgat tttgtactgg gtgacgaagg acagcaggtt
     1321 gcttccgaag ttggtctgat cacagtaaaa taatagcccg acacagaaaa aggaaaaaag
     1381 cgatgaatag tggaacacac gcttccgtaa tgggaaacag aaaaaataga gcggtcgttg
     1441 aaactgtggc acaaatagta tttaccattt gtgccttctt cgccgtactg gcggtagttt
     1501 ctatcacctt atatatgatc atcagcggta cccccgctat tatgaaagta ggtctgggag
     1561 agatcctgtt cggcacggaa tggatgccaa cggcggcaga tccgaaattc ggtattctgt
     1621 gggttatcct gacttccatc gtcggtacga cgctggctat cctgctgggt gtgccaatcg
     1681 gcgtgctgac ggcagtattc ctggcagagg tggcacccag acccttagct gccattgtaa
     1741 agcctgcggt agagctgtta gccggcatcc cttccgttat ctacggactg ttaggaatct
     1801 acttactgaa tcctctcatg tataaattag aaagactgat cttccaggga tccacgacac
     1861 accagttcac cggcggtgca aacctgctgt cagccgtgat cgtactggcg atcatgatcc
     1921 tgcccactgt gatcaatatc agtgagactt ccatccgggc ggttcctgca ggaatcaaat
     1981 cctcctctct ggccctggga gcctcccgtg tacagactat tttccgctcc atccttccgg
     2041 cagcaaaatc cggtatcgtg acggctatcg ttctgggagt gggacgtgcc atcggcgagg
     2101 ctatggcgat cacactggta tccggtagca gcgtcaatgt gccgttgcca tttaactccg
     2161 tgcgattcct gaccacagct attgtcagcg agatgggata ttcttccgga ctgcacagac
     2221 aggtgctgtt caccatcgga ctggtgttgt ttgtatttat tatgatcatc aatatcgtac
     2281 tgaataaaat actgaaaaag ggggcggatg acaatgaata aggacacctt atatgtaaaa
     2341 agaaaacgcc ccacagacat gcttttgctg gcagccattt atgtctctgc cacactggcg
     2401 gtggcacttt tactgggaat tatcctgtat gtcttcgtaa agggcatccg caccgttaac
     2461 tgggcatttt taacgacggt gaccagtgcc agaaagcaga ccaccggtat tgcgggcaac
     2521 ctgttgaata ccctgtacat catcgtgatc actctgttga tcgcgacccc catcggcgtg
     2581 ggaagtgcca tttatctgaa tgaatatgca aaacccggca agctggtctc catcattgag
     2641 ttcactacgg agacactgtc cggtattcct tctattattt tcggcctgtt cggtatgatc
     2701 tttttcggag agactttggg cttcggctat tcactgctca ccggttcctt taccctgaca
     2761 ctgatggtgc tgcccctgat cacaagaaat acgcaggaag cattgcgcac cgtgccggac
     2821 agttatcgta ccggtgcgtt gggaatggga tccacgaaat ggcatatgat ccggacgatc
     2881 cttctgccgt cttccatgcc aggtatcgtc accggtatca tcctggcact gggacgtatc
     2941 gtgggagagt ctgcggccct gttgttcact gcgggaagcg actacaagct gccgaagttc
     3001 accggagttt tcggagacaa tctggcaaag ctggctcaca aggccatgga gtctggtggt
     3061 accctgacca tcgaattgta tttacagatg cagaagggac agtatgataa tgctttcggt
     3121 atcggttgtg ttctgatcat tatcgtattg atcctgaacc tcctgaccaa gctggcggcg
     3181 aataagctta gacgagagta agagaaatca caagaaggag aaaatatatg gctaacagta
     3241 aaatatccgt ggaaaactta aatctccatt atggagagaa ccacgcactg aaagatgtaa
     3301 atatggagat cgcagaccac gccattaccg catttatcgg tcccagcggc tgcggcaaat
     3361 ctacttttct acgttgcctg aaccggatga atgacctggt ggacggctgc agagtcgaag
     3421 gaaaagtaat tctggatgga gaggacattt acgataagcg ggtggatacc acactgcttc
     3481 gtaaaaaggt cggcatggta ttccagcagc ccaacccttt tcccatgtcc atttatgaca
     3541 acatcgccta cggtcccaga ctgcacggca tcaagaataa aaaggaactg gatgagatcg
     3601 tggagcgttc tctgcagggt gccgctatct ttgaagaggt aaaggatcgc ctgcacaaat
     3661 ccgcgttggg actgtccggt ggacagcagc agagactgtg tattgccaga gcactggcag
     3721 tatctccgga ggtacttttg atggatgaac ccacctccgc actggacccg atctccacgc
     3781 taaagatcga ggaactgatg gagacactga agaaaaaata caccgtggtc atcgtaaccc
     3841 acaatatgca gcaggcagcc cgtgtgtcgg atgacaccgc atttttcctg gtaggggaag
     3901 tcatagagaa aaatgcaacc tccgagatct ttgcaagacc tcaggacaaa cggacagaag
     3961 actacatcac cggacggttt ggatgatttt ggatgagaag ataggaggaa tgggagatgt
     4021 cacccagagt attatttgaa caggatcttg agactttgaa aagtaaagta tctgagatgg
     4081 gagagcatgc ggagatcagt tatgaccgta tggtgtatgg aattcgtgag aacaaagaag
     4141 acattctgaa gacactgttg aatacagatc ataccatggt ggatatgcag cgaagtatag
     4201 aagctatgtg tctgagcctg ctgaccagac agcagccggt ggccagagat atgcgtctgg
     4261 tatccgcagc gctgaaagtg gtaccggata ttgaacggat cggagaccat gtggcagata
     4321 ttgtggaact gtacctgcgt atgggcaaca tgaatcccga aggaaaacag gaacatctgt
     4381 tgctggagat gatggaagaa gccaaggaga tgacccacaa ttcggtggag gcatttgtgg
     4441 aaggtgatga ggcaaatgca agaaaagtag tagaacatga tgatgtggta gatgatctct
     4501 tcaacgaagt caaaacggaa atgatgcagg ctatcagaga gcagaacctg gatgcggatc
     4561 gtgtggtgga ttatctgatg atggcaaagt atctggaaaa agtgggcgac catgcggtaa
     4621 atatcgcaga gtgggccatc ttccgcatga ccggagatat ggaaggtgcg agattgtatt
     4681 aggattcgat aatctcggta acgatgcaga gccccaattt gcaaaactnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnngc tcctgcggag cgttcctttt gctcattgag
     4861 gcttgctcgc caaagaacta ttgccatata ctcttacaga tgctggcatc tgataatccc
     4921 tgtaacgatg cagagcccca atttgcaaaa ctgctcctgc ggagcgttcc ttttgctcat
     4981 tgaggcttgc tcgccaaaga actattgttc tatgctctta cagatgccag catctgacga
     5041 gcatagaaca atagttcatg gctctgcatc gttacaactt tacataaatt ttacattcac
     5101 tggtaacgca ttttacataa gcaagataaa gtgtagatag aaaccaaaaa tgtaaagtat
     5161 gttatgtaaa atgagaggat tagagtatga gcttttttga tttactgaag atggtaggag
     5221 gacttgcatt attcctgtat ggaatgaatc tgttgggtga cggattgtcc cgggcatctg
     5281 gcggacgatt agaacgcatt ctggagaaac tgaccaacaa cccgttaaag gcagtgctca
     5341 tgggtgcggt ggtcaccggt gtgatccagt cctcctccgc taccactgtt atggtagtcg
     5401 gatttgtcaa ttccggtatt atgagtctga aacaggcagt aggtatcatc atgggtgcca
     5461 acatcggtac cacggtgacc tcctggatct taagtctgac gggcattgag agcgatgctt
     5521 tctggatccg tatgctcaag cccagttctt tttcaccgat tttagcgatc gtgggtgtgg
     5581 gtattttgtt attttctcat aaggataaat taaaaaatgc agcaactatt ctggtgggat
     5641 ttgccattct gatgtttggc atggattcca tgagcgcggc agtttcaccg ctggcagagg
     5701 taccggagtt taccaatctg ctgatcgagt tccaaaatcc cgtattcggt gtgctggcag
     5761 gactcattct cacagcggtg atccagtctt cttccgcatc cgtaggtatt ttgcaggcac
     5821 tgtgcatgac cggagcggta agctttggcg cagcagttcc cattatcatg ggacagaata
     5881 tcggtacctg tgttaccgcc atgatctccg gtatcggtgc ttcccgcaat gcaaaacgtg
     5941 cggctctgat tcatctgtat tttaatatta tcggaacgat cctgttcctt ggagtgttct
     6001 atacggtcaa tgccattcat ccctttgctt ttctgggaga agtggcaaca ccttttggaa
     6061 tcgcagtagt tcacagctgc ttcaatatcg cggctacgct ggtattgctt cccttctcca
     6121 acggtctggt gaagctggcc tgtctgacgc tgccgaccca tgaggacgtg aaggaaatgt
     6181 ccgagacaga caagacttta agcttactgg atgtacgttt cctggagaag cctgcctatg
     6241 ccgtagagca gagcagactg gtggcttctg tgatggcgga taagtccagg gaagccatgg
     6301 atctggctat ggagctgctg cataatttcg atgaggcgaa agcagagcag gtggcacaga
     6361 tggagacgga tgtggaccgc ttcgaggatg aactgggaac ctatctggtg aaattgagtt
     6421 ccaaacagct ttccaataag gatagtgaga ctttaaatac gattctgcac tgcatcggcg
     6481 attttgagag aatttccgat catgcccgca acatcaagga agcggcacag gagatgcatg
     6541 acaagcagct ggtattctcc gagaaggcgc aggaggaact gaaggtattc gagagagcgg
     6601 tacacgatgt gctggatctt accatgacca gctttaagaa cggagatctg aatctggcaa
     6661 gacaggtgga gccgttggag gaagtcatcg atggactgag cctggatgtg aagcagcgcc
     6721 atgtacgcag actccgtaag ggaaaatgta ccattgaact gggcttcatc ttatccgatg
     6781 tcattacgaa ctttgagcgt gtcagcgacc actgctccaa tattgcagtc tgtctggtac
     6841 aggtgaatga ggatgaattc gatacccacg cttacctgga tgaactgcgc caggaagata
     6901 acaaggactt ccagagcaaa gtgctggcat gccgtgagaa ataccagtta ccgcccacaa
     6961 agcaggaacg tcttgcggaa aagaatgcaa aactgcttgc ggagtgagga tttaccgagg
     7021 tatagttaag aggagaaagc acagtcagag aggagtgctg tgagatggct ttgatctatg
     7081 tagtagagga tgacaaaaat attcaggaga tcgagacctt tgccctggcc aatgtgggct
     7141 atgaggtaaa aggttttgat tccgccatgg atttctatga ggcgctgaat gataccacac
     7201 cggatctgat tttattagac atcatgctgc cggacgagga tggtctgagc attgtgaaaa
     7261 agctgcgtac cagacaggac acggtgctgg tgccggtgat catggtcacg gcgaagacca
     7321 cggagatcga caaggtcaag ggactggatc tgggggcaga tgattatatt acgaagccct
     7381 tcggtgtcat ggaactgatt tcccgggtaa aggcaatgct caggagaagt cagaagagtc
     7441 aggaaaaaga taaggtgctg aagctgggga acattgtgct ggaccgagag aaacattctg
     7501 tcacggtagg caaggagcct gtggagttga cctacaaaga atatgagctg ttaaagcttc
     7561 tgatgaccaa tgcgggcatt gtgaccacca gagaactgat cctggaccgc gtctggggga
     7621 tcgattttga gggagaatcc cggacgttgg acatgcatat caagacgctg cggcagaaac
     7681 tgaaggaaga gggcagtctg atcaagaccg tgcgtaatgt gggatacatt atgaacggcg
     7741 agaaagcgta acagaagatg aagaaaaaga ttaacaaaat gatgatcctc attgccacgg
     7801 taacgatttt gcttaccatg gtactgatca cagtcgtgta ttatgatctg ttccgcagac
     7861 aggtgctgga ggatctgaaa agctatggtt ccctgttaaa aagctgttct tcggtagaag
     7921 agatcaagga aaacggagtg ccggtggaat cggatctgcg attgacgctg atcggcagtg
     7981 acggacaggt attgtatgat aatgaggcag attctgtggc catggggaat cacgcttccc
     8041 gtccggagat ccaggaagcc atgcgggacg gcgaaggtga agcagtccgg aattccgaga
     8101 ccttgtccca gtccaccttt tattatgcca tccgcctgga agatggcagc atcctgcgac
     8161 tgtccaagga tgccagaagc atttacagca ttttcagtca ggcgctgccc atgatggtgg
     8221 ggatctttgt ggtgctgctg gtgctatgca tggtggcggc cagagtgctg accacgaagc
     8281 tggtggctcc cattgagaaa ctggcacaga atatcgggga atgtacagag atgcagacct
     8341 atgaggaact gacaccgttc atgaccatga tccgcaaaca gcatgaggac attatgaaaa
     8401 atgccagaat gcggcaggag ttcagcgcca atgtgtccca tgaactgaag actccgctga
     8461 catccatctc aggatatgcg gagctgattg agaccggaat ggcctccgaa caggatacgg
     8521 tgcgttttgc tcacgggatt cataacagcg caaatcgtct acttacattg atcaatgata
     8581 ttattcgttt gtcggagctg gacggaacgg aagaggaagc ggatacggag cttttgaatc
     8641 ttcatgaaat ggcgcagaat tgtgtggaaa tgctgaaaat gagtgcagag aaacacaagg
     8701 tgaccatagc gctgaacggc acggaatgct atgtgacggc caatcgccag atgatggaag
     8761 aacttttata taatctgtgt gataacgcaa tccggtataa caatccggga ggaagtgtgg
     8821 atgtgcagac ctatgccaga gaggggcata cctatctggt ggtaaaagac accggcatcg
     8881 gcatctccaa ggagcatcag gagaggatct tcgagcgctt ctaccgtgtg gacaagagcc
     8941 gctccaaatc caccggcggc acgggactgg gccttgctat cgtaaaacat atcatcgcca
     9001 agagccacgc tgaactggaa ctggaaagtg aacccggaaa aggaacgacg atacgggtga
     9061 ttttctgaga acttcgagtt caaattttct aagacttcat tcgagagagt ggagtctttt
     9121 ttacgcatga tattccgcca gcagctgttt cgcataagca acacagtctc tgtgcagagc
     9181 aaattttttg ccatcgcaca tgatgacttt gcggaagccc agcttctcca tgtatctgcc
     9241 gttaaggatc gtcttgacgg tgggagcgaa gaaggtaggg tgattctcta tctgagagaa
     9301 caaattgata gcggtggtgg ctacttttac ggtgcggccg tccttcaggg tgacgatgac
     9361 attccggcct tcttcttcgg cgtagaggat gtccggtgcg gtgacataga cggtatcttc
     9421 atcctctcga agctctatga gaggaactgc cttagacata atatccagag catggtcaat
     9481 actctgtccc agttccgcta ctttgatggg cttgtccaga aaaataacat tttccggata
     9541 ggtctgttcc ctgtaattga tcttggaaca gcacagcacg atgggtgcgt tgaccccctg
     9601 ggcaatcagc gcatttgcca cttctgtacc ggtgatgcct tccgtcagac acatatcaat
     9661 atagaaggca tcgtattgca tgggattgga cagcagtgag gtagaattac cgaaagaatc
     9721 ggtataaatg atacccgtag acgctgctct tttgtcggat tcccgcttta acagacgctc
     9781 cagctgcttt ctgtcggcta cattatcatc acacactgct atatgcatat cgatacctcc
     9841 cggactttat ctcatataaa agaagtatag catatttcct atttgaataa aagggcaaaa
     9901 agcagtacct gtaccaccag gatcgccggc attccgtact tgaaatacca gtgcttcgtc
     9961 ttatgacgga agtgctgcat tcccagaata catcccagac tgccacccaa aagcgccgtg
    10021 aaaaaaagag aagcctccga gatgcgccag gcaccccgga tggctctttt tttatcgaca
    10081 cccatgatca catatcccac aacattgatg atcaccagat acaggatcac cacagtaatt
    10141 atagtctgtg cagttatcat agacacctca ttattttaat tcttagttac caagtttctt
    10201 ctcgttctcc tgtaacttca tggcacgaac cttcagagac agaccgaaca gattgatgaa
    10261 gccttctgca tcgtgatggt cgtacagatc accggtggtg aaggaagcca ggttctcatt
    10321 gtacagagag taaggagaag tggttccggc cttgatgatg ttgcccttgt agagcttgaa
    10381 ctttacttca ccggtcacat attcctgggt tttctcgatg aatgcctgct ctgcctcacg
    10441 aagaggagtg aaccatttgc cttcgtatac cagactggcg aatttcgcac ccatttcctt
    10501 ctcgaatgca taggtatcgc gatccaggat cagctcttcc agctgctggt gagcctccat
    10561 caggatggta ccgccgggag tctcatagac accacgggac ttcatgccaa cgacacggtt
    10621 ttctacgata tctacgatac cgataccgtg cttgccgccc agcttgttca gggtacggat
    10681 gatatcggag accttcatct tctcaccgtt aacggaagtg ggaacaccct tttcaaaggt
    10741 catggtcaca tattcaccct catcgggagc cttctcggga gtcacaccaa gtaccagcag
    10801 atgctcgtag ttaggctcgt tgccgggatc ttccagttcc agaccttcgt ggctgatgtg
    10861 ccagatatta cggtcacggc tgtaggaaga atcggtggag aaaggaagat gaatgccatg
    10921 agccttgcag tattcgatct cggattcccg ggagtccatg ttccatttgt cgcttctcca
    10981 gggagcgatg atcttgatat cgggagccag agccttgatg cccagctcga aacggatctg
    11041 gtcgttacct ttaccggttg caccgtggca gatggctaca gcgccttctt ttctggcaat
    11101 ctctaccagt ttcttggcga tcaggggtct tgccatggaa gtacctaaca gatacttgtt
    11161 ctcgtatacg gcatgagcct gtacacaggg aacaatataa tcatcacaga attcatcgat
    11221 aacgtcttca atatacagct tggaagcgcc gcaagccttt gctctctctt ccagaccgtc
    11281 cagctcctct gcctgtccgc agtttacgca gacacagata acttcatagt caaaattctc
    11341 cttcagccag ggaatgatag cggtagtatc cagaccgccg gaataagcca aaactacttt
    11401 ttccttcatg ataaaatcct ccgttttata gtgtgtatga tgccaggggc aaaagccctg
    11461 aaaatgcgat gtgcaaagca cgaagcaatc tgtaagtatc agatgccagg atcgtgtgtt
    11521 acgctctgac aattactata caacattata atcgcaaaag caagcagaaa agttgcataa
    11581 acaaatcata aaaatgaata aaataaatta aaatatgcac ataaatgcat agaataaaaa
    11641 atatacagta tatttatgca taaaatatgc tgcctgatgc atagtacggt aacgaccata
    11701 taagatgcgc cggaatcgtc aaaagtcagt cgctgatctt ttcaattccg gagatatcac
    11761 tgtcgatcat cagagagtag ttcgtataat agaccacgaa gccaggcttg gcaccggccg
    11821 gctttttgac ctgttttttc tggacatagt cgatctccac cttttcctgc tccctgccct
    11881 tagaatagta ggcagcaagc cggccggcct cttcaaaagt ccggtccggc agttccttcc
    11941 cttcggtctt taccactaca tgggagccgg gaataccttt ggcttggaac gaccagtcat
    12001 ttccgttggc gaatttgaag gtcagttcat cgttctggaa a
//
LOCUS       DAWDHD010000070        11949 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_345_length_11949_cov_9.694888, whole genome shotgun sequence.
ACCESSION   DAWDHD010000070 DAWDHD010000000
VERSION     DAWDHD010000070.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 11949)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11949)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11949
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_345_length_11949_cov_9.694888"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3445 a         2535 c         3462 g         2507 t
ORIGIN      
        1 ccatgaatca ggcgctgaag actgtactgc gggaaaaaca gtcggtaaaa aacctggaac
       61 acagtcagct ggaacgtctg atgaacagag aagagatttt gtcattatta ggagaatgtg
      121 aacggaaaaa acaggtggcc aaagcaagac tgttaaaagc cctgtcagaa caggagacga
      181 ttccctatga gtggatcacc ggaaaactgg gggtcagtgc agccacggta aacagtattg
      241 taaaaagtgg cgcggcaaaa ctggtcagtt ccgaaagcta tcgcaatccg gtcaaaatgc
      301 agtccgtaga ggcagtgcgt aatacgctga gtgaggaaca gcagaccatc gttacggaag
      361 tgtttgcgga ttttgatgcc ggaaggagac agacctatct gatccacggc atcaccggca
      421 gcggcaagac ggaagtatac atgcggctga tcggagaaat gatcgcgagg ggacggcagg
      481 ctattgtgct gattcccgag atcgccctga cttatcagac actgctgcga ttttaccaga
      541 gattcggtga tcgggtcagc gtgatgaatt ccacgttatc ccccggggaa aaatacgatc
      601 agtgcgaacg ggccaaagct ggagaaatcg atgtgatcat cggcccaaga tccgcacttt
      661 ttacgccgtt tccaagtctt ggcctgatcg ttatggatga ggagcaggag aatagctata
      721 aaagtgaaag tactccgaaa tatcatgcca gagagactgc gctggaagtg gcaaaactat
      781 acggggccag tgtagtgctg ggttcagcca caccttccct ggaggcctat tatcgtgccg
      841 gtaggggaga ataccggctg tttcaactga caaagcggct caccggcggg gaacttccca
      901 cggtgtacac ggtggatctc agacaggaac tgcaggaggg taaccgttct attttcagca
      961 gaaaattgca ggaactgatg acagaccggc tgaacaaggg ccagcagacc atcctgtttt
     1021 taaaccgaag aggctacgcg ggatttgtgt cctgccgttc ctgcggtgag gtcatgaagt
     1081 gtccgcactg tgatgtctct ctctcggaac ataagggagg aaggctgatc tgtcattact
     1141 gtggttatac acagccgatg ccgaagcttt gtcccaagtg tggatcgaaa tatattctgg
     1201 gatttaaggc aggcacccag cagatcgaag aaaaattaaa ggagctgtac cccggtgtgc
     1261 ggacgttgcg catggatgcg gataccaccc ggacgaagga cagctatgag aaaatattat
     1321 cctccttcgc aaacggggaa gcggacgtgc tgatcggcac ccagatgatc gtcaagggac
     1381 atgatttccc aaaggtgaca ctggtgggga tcctggcggc ggatctgtcg ttaaatgcgt
     1441 cggattaccg ggcgggagag cggactttcc agcttctgac ccaggcggtg ggccgggccg
     1501 gcagaggagc gctccccggg gaagcggtga tccagaccta tcagccggat cattacgcca
     1561 ttacctgtgc tgcggctcag gattacgaga acttttatca ggaggagatt ctgtatagag
     1621 agctgtccgg ctacccaccg gtggctcata tgctggcagt acagatctac gggaaagagg
     1681 aagaaaatgt aaaacagctt gccctgagac tgacagatgt ggtaaagtct aatatggact
     1741 ttttgtcagg aaagcaaaaa gaacagtcca ataagaagat acaggtactg ggacctgccc
     1801 ctgccaacat cagcaagatc aatgatatct accggtatgt gttctatgtg aagcatgcgg
     1861 agtatgatgt cctggtggaa gtaaaggaca gactggagga aactctcaga cagtggcagc
     1921 ccagacagat gagtatacaa tttgattttg acccggtaag cgtgttatag gaggtaaaga
     1981 actgtggcaa tcagaaacat tcgtgaatat ggggatgaag tactgaccaa gacctgcaaa
     2041 gaggtcaagg agatgacacc ccggaccaaa gaactgatcg aggatatgtt ggagactatg
     2101 tacgatgcca atggcgtagg gctggcggca ccacaggtag gtgtcctgaa gagaatcgtg
     2161 gtgatcgata ccaccggaga ggatccgtat attctgatca acccccgcat cgtagagagc
     2221 agtggagagc agaccggaca ggaagggtgt cttagcgtac caggtaagtt cggcatcgta
     2281 accagaccca attatgtgaa agcggtagca ctggatgtga atatgaagcc catcgaagta
     2341 gaaggaacag aattgctggc cagagccatc tgccacgaac tggatcatct ggacggtcat
     2401 ctctatgtgg aaaaggcgga aggaccgctg caggatatga cctacgagga tgaggaagaa
     2461 gagtaagctt tccggaaagg agacagacta tgcagatcgt atttatggga actcccgatt
     2521 ttgccgtggg agcgctggag gcactgatca cacagggcta tgagatcacc gcagtggtga
     2581 cccagccgga caagccaaag ggcagaagca aagaattaca gtttcctccc gtgaaggaat
     2641 gtgcggtaaa acatggcatt cctgtattcc agccccgtcg gatcaagaca ccggaagcca
     2701 ttgaggaact gaaaaaatat cccgcagata tttatatcgt ggcggcgttc ggacagatcc
     2761 tgtctcagga gattctggat ctgccgaaat acggatgtct gaacatccat gcatccctcc
     2821 ttccgaaata ccgcggggca tctcctatcc agagagtgat catcgacgga gaggccgaga
     2881 ctggtgtgac catcatgcag atgaatgcgg gactggacac cggcgatatg ttgtaccgga
     2941 agaaaatccc cattacttcg gaggacacct tcgaaacttt gcatgacaag ctgatggtgc
     3001 tgggcgggga agcgatcacc gaggcactgc ctctgctgga ggcaggcaaa ctggtaccgg
     3061 aaaagcagga tgataccaag acctgctacg cggctctgat caccaaagag atgggaaagc
     3121 tggactttac gaaaacagcg gcagaactgg atcgtttgat ccgtggactg actccctggc
     3181 ccagtgcata tacgcattat catggaaaac agctgaaaat ctggaaagcc attcctatgg
     3241 caactgcaca taaggaagca ccgggcacga tccttatggt gaataaggat tcctttgaag
     3301 tggctgcatc ggaaggctcc ttaaaggtga tggaactgca gctggaaggt aaaaagcgta
     3361 tgacaactca cgatttcctg ctgggggtaa aggtacagcc gggagagctt ctgggcgagc
     3421 gttaggatac gacgaaacga aaagcaacga tcgggtaagc ggtgttgaca gaaaggagac
     3481 tttcgtatgt acggtggcta ttacggatat ggttattatt gggatcctac ttattttctg
     3541 gtactggtcg gactggtatt gtgtctgggt gccagcgcac tggtgaattc caccatgcgc
     3601 aaatacagca aagtccggaa tatgagagga ttgacgggag cggaagcggc ccgcaacatt
     3661 ctcaacagag aaggcttata caatgtacag atcgagtgcc tgaactccga tgacggtgat
     3721 cattacgatc cccggacgga tacggtgcga ctgtcttaca acaactttaa cggaacctcg
     3781 gtgaccgcgg tcagtgttgc ggcccatgag tgcggacatg ccattcagca tgcccatggc
     3841 tatgccccca tgagcattcg tgctgcactg gtgccggtgg tgaatatcgg aagcaattta
     3901 agcattcccc tgatcttttt aggagtactg ctcagctgga accagacatt gatccagctt
     3961 ggtatctggg catttgccct gaccgttatt ttccaactgg tgacactgcc tgtggaattc
     4021 aatgcttccc acagagctat cgtgaaggtg gaagaatacg ggttattagg cagtgaagaa
     4081 gtaagcggcg gcaaaaaggt gctgacggca gcggctctga cctatgtggc aggagcggcc
     4141 tcctccgcat tacagctgct ccgactgatc ctgctgtacg gcggcaatcg cagaaggaga
     4201 gactaatctt gagtgatcat gcaacgaccg atacaagaga gattatagtg gagatcctgc
     4261 tcagtctgga aaaggggcag gatttttccc ataaattgat gaaagcggtt ctggataaat
     4321 acgattatct ggatcccaga gagaaagcgt ttatcaaaag agtcacggaa ggtacgatag
     4381 agagacagct ggagctggac tattatctgg atcggttttc ttctgttccg gtccggaaga
     4441 tgaagcctct gatccgctgt cttcttcgca tgagtattta tcagatcctg tatatggacg
     4501 cagttcccga cagtgcggtg tgcaatgagg cctgcaagct ggcggccaaa agaggcttcc
     4561 gtaccctgaa aggcttcgtc aatgcggtgc tgcgtaatat ttcccgcagc aaggaacaga
     4621 tgccgttgcc ggatcccaag gatacggtga aatatctgag cataaaatat tccatgccgg
     4681 aatggatcgt gaatctgtgg ctgcctgcat atggcagaga aggaacggaa acacttttaa
     4741 aaggactcct ttccattcat ccggtatccc tgcggttctc cacggaactg accgaatcgg
     4801 agagagagtc actggcggag aaaattgaga aaaccggtgt acgtctgcag caaagcaggg
     4861 aacttcctta tgtgtatctg gcacagaatc tggaaaatgt aagtgaactt ccgggctttg
     4921 cggaaggcaa atttaccgtg caggatgcca gctccgcact tgcagtggaa gcggcaggta
     4981 tatgcccagg taatacggtc atggatctgt gtgcggctcc gggtggcaag acgatcctgg
     5041 cggcggaatt tgcaggggaa gcaggccaca tcgtgtcccg tgatgtatcg gaatacaaga
     5101 cggacctgat ccgggagaat ctggagcgca tgaaccgtag gaatgtggag gttcaggtgt
     5161 atgatgccac ggtgcatgat ccggagaagg aaaaatatgc ggacgtggtg ctgatggatg
     5221 tcccctgctc tggtcttggt gttatgggaa agaagagaga tatcaaatat catgccacac
     5281 cggaaagcct gcaatccatc acagaactgc aaaggcagat cgtggcagcc ggatggcagt
     5341 atgtgaagcc gcacggtgta ctgctctaca gtacctgcac gatccaccgc ggggaaaatc
     5401 aggagatggc agtctggatc acagagaatt tcccgtttgt actggaagaa gagagacagc
     5461 ttctgccggg aacggatgag accgacggat tctattatgc aagactgcgc agaaaagcgt
     5521 gagagataag aagtaagaga acgaaaaatg gatacgaaaa aagatatcaa atccctgaat
     5581 ctggaagaat tgaagacaga gctggagagc atgggagaga aggccttccg ggcaaagcag
     5641 atgtatgaat ggatgcatgt gaagctggtc aggagctttg atgaaatgac caacctctcc
     5701 gcaaaattca gggagcaatg taaggaaaaa tatgaataca cctgtgtaaa ccctgtcaga
     5761 atacaggagt ccaagatcga cggaacgaaa aaattcctgt ttgaattatc tgatggcaat
     5821 gtggtggaaa gtgtctggat gcagtataag cacggcaaca gtgtgtgcat ttcttcacag
     5881 gtgggatgcc gtatgggctg taaattctgt gcgtccacgt tggacggact ggaacggaat
     5941 ctgactcctt ccgagatgct ggatcagatt tacgccatta caagactgac gggggagcgg
     6001 gtctccaatg tggtagtcat gggcactggg gaaccgatgg ataattatga caatattttg
     6061 aaatttctgc atcttctgac cgatgagaac ggtctgaaca tcagccagag aaatgtcacg
     6121 gtgtccacct gtggaatcgt gcccagaatg cggcagctgg cggatgaaaa gctgcagatc
     6181 acactggcac tgtccttgca tgccaccacc gatgaaaaac gaagaaaact gatgcccatt
     6241 gccaaccggt attctatcaa agagttgatg gaagtctgtc agtattattt caaacagacc
     6301 ggacgaagga tcactttcga atacagtctg gtgggcggtg tgaatgatac ggaagaggat
     6361 gccagggaac tgatcgccct ggcgaagccg ttgtgctgcc atatcaacct catcccggtc
     6421 aaccccatta aagagcggga ttatgtgcag tcggatacga agcacattca ggcatttaaa
     6481 aataaacttg aaagtaagat gatacatgtt acaattagaa gggagatggg ccgggatatt
     6541 gacggagcct gcggacagtt aagacgccgt catacgcagg aggcgaagaa cccgggggaa
     6601 ctctcacagg aggaagtttg acgtgcttaa gacgttttcc ataaccgata tcggaaggaa
     6661 aagaaaagta aatcaggact atatctatac ctctgaaaaa ccggtgggaa atctgccgaa
     6721 tgtatttatc gtggcagatg gtatgggtgg tcacaatgcg ggagactatg cgtccaaggt
     6781 aacggtagag accatgcttg ccgagatgga gaattctttt gagaagaatc ccaccctgat
     6841 cttccgcaaa gccattgagg aagccaatga tgtgatccgc aagagagcca gcgaggatga
     6901 aaaactggaa ggaatgggga ctacggttgt ggtggcatcc tgcatgggca gattcttaca
     6961 ggtggccaat gtaggagaca gcagactcta tgtggtgggc agcaagatcc gccagatcac
     7021 aagagatcat tcgtatgtag aagaaatggt acggatcgga gaactggatc gtgccgatgc
     7081 aaggaaccat ccggataaaa atattattac cagagcggta ggggcggaag acaaagtgga
     7141 gccggatttc tttaccgtgg aattacagga gggtgagatc gtccttatgt gctcggatgg
     7201 tctgacaaat atgctggaag acgaggaaat ccgcatgatt atcagcggag ccagagacct
     7261 tgtggagaag gcagagagcc tggtggaggc ggccaatgcc aacggcggca gagacaatat
     7321 cagtgtgata ctgatcgagc cgtgcgcgta aattacccag cttttttgga aatggagagc
     7381 aagtatgatc aaaataggaa tgatgatcgg cgatcgctac gagatcctgg aaaaaatcgg
     7441 aaccggcggt atgtcagatg tctataaagc aaaatgtcac aagttgaatc gctatgtggc
     7501 gatcaaggta ttgaagcagg aattcagcga aaatgctaat tttgtatcca aattccggat
     7561 cgaggcacag gcggcagcag gactgatgca ccccaatatt gtcaatgtct atgatgtcgg
     7621 ggaagagaac ggaatctatt atatcgtcat ggaactggta gagggtatta ccctcaaaaa
     7681 gtacattgag aagaaggcca gactgtctta caaggaagcg gtaagtatcg ccatccaggt
     7741 aagcatgggt attgaggctg cacataataa tcatattatc catcgggata tcaagccgca
     7801 gaatattatt atctccaagg acggcaaagt taaggtgacg gacttcggta ttgccaaggc
     7861 agctaccagt aataccatca cttctaatgt catgggaagt gtacactata cttctcccga
     7921 gcaggccaga ggggggtaca gcgatgaaaa gagtgatatc tattcgctgg gtatcaccat
     7981 gttcgagatg ctgacgggaa gagttccttt caacggggag actacggttg ccattgccat
     8041 caagcatatt caggaagaaa tgccgtctcc gaaggagttt gtaccggaga tcccttccag
     8101 cgtagccagt attgtactga aatgttgtca gaagtctccg gaccgcagat accagaatat
     8161 ggcagaactg atcgcggatc tgaagcagtc cctgatcaac ccggaggaag atttcgtggt
     8221 gaacaccgat ccggatatgg aaggtggtac ccggatgatc tccgatgagg atatggcgca
     8281 gatcaaacga aaagtccctt atcagggagg acgggaatac gaagaaccgg atcatgatga
     8341 gaccatgcgt ctgcgttccg ataccgaagc catgtatgaa aacgaagaag atggcgaaga
     8401 cgaggataat tacgactacg accccaagat ggagcgtgtg accacaatcc tggcaattgt
     8461 agccggtgtg gtgatctgta ttatcctgat cgttctggca gtgaagatct tcggcggcaa
     8521 aaatgaagga agcagtgact ctgatgcagg accgatcatt acgaccgaag agacggatgc
     8581 cacagaagtg gaaaaggtct ccatgccgga tgtaaagggc atgaaggtag aagatgcaaa
     8641 gagcaccctg accagtctgg gaatcctcac agaggtgact tatgaggagt ccgacacggt
     8701 agcggaaggt attgttatca atacagatgt cagcgtggga gcacagatcg ataagggcag
     8761 caaggtaacc ctgaaagcca gtgcaggaac tcagggtgtg gaagtaccca atgtggtgaa
     8821 tcttactttt gatgaagcca atacacagct ggtaagcaag ggattcctgg tcaacaagac
     8881 agagaactat tccgatacgg tagagtccgg ccgggtgatt tcccagaccc cctccgaggg
     8941 caacaaagca ccaaagggca gtgtgatcac tgtgacagta agtctgggta aggaagatac
     9001 caagatccgt gtgccgaatc tgatcggtct gtccgagcag gacggcacgg tagaagccat
     9061 cgaggcagga ctgaccgtag gcagtgtgac ctatatctac agtgacgaag tgggagaagg
     9121 catgatctgt taccagagct attcccatgg ctcctatgtg gagcagggaa cttccattga
     9181 tatcaaggtc agtcagggag ctcagaaggt gacttacaag tgtaatgtca gcattgcggc
     9241 accgacgact tcggaagcac ccggctatgt atcgggaatg gaagtcagca tcaaactggt
     9301 gacggatgac aataacgtac tgctggatac caagaccagc actttcccgg tagctaccaa
     9361 tttcaacggt atgactgcct ccggtggtac gctcaccata acgtatcagg tgacaagcga
     9421 accgaccacc acaacgaacc cggataccgg ggagcaggtg acggttcccg gaacgacaga
     9481 agaccgttcc tttaccagaa gagttgagtt cgtaaaggaa taagatcaaa ggcgggaagc
     9541 tatgaagggc aagattataa aaggaatcgc cgggttctat tacgtccatg tagcggacga
     9601 tggccataca atgggcacgg tgtacgaatg caaggcaaaa ggagtctttc gcaaggatca
     9661 cagaaaaccc ctggtcgggg atgacgtgga gatggatgtc ctggatgagg cacagaaaaa
     9721 aggaaatatc agggaattgc ttcccagaca cagtgaactg atccgtccgg cggtagctaa
     9781 tgtggatcag gcgctggtga tctttgccat cacgaagcca cagccgaatt ttaatctgct
     9841 ggatcgtttc ctggtcatga tgcagcagca ggacattccc tgcattatct gttttaacaa
     9901 gcaggatatt gacgaggaag gcaaaaagga agattatcgt gccatttacg aacaggcggg
     9961 attccgtacg atcacggtca gcgcagccag acaagaaggg atagatacca ttcaggagct
    10021 gcttcgcggc aagaccacta cggtggcggg tcccagcggt gtcggtaaat cctctctggt
    10081 aaactgcctg cagtccggca cagtgatgga gaccggctcc atcagtgaga agatcgagcg
    10141 gggaaagcat actaccaggc actccgaact gatctccatt gcacaggata cgtatattct
    10201 ggataccccg ggatttagct ccctggggtt gtttcggttg gagaaggagc agctggcgga
    10261 attttatccg gagtttgccc cttttgaaaa atattgcaga ttcggaggct gctcccatat
    10321 ctctgagccg gtatgcggta tcaaggctgc tgtggcagag ggagagatcc cgcagatacg
    10381 ctatgagaac tactgtctgc tctatgatga actgaaaaat cagagaaaat attaattatg
    10441 agtgaagtgc agaataatct aggggaaaaa ttcaaagaga ccaatgcgga agcgatgaag
    10501 accggagatt ttcatgcgtt gaacacgctg gtgacggata cggtcaacga tgccttgcgg
    10561 gaggcggatt tccaggcaaa tctgtccagg gaagccacgg aaaaaaaggt gtggaattcc
    10621 cagcgcagct ggcaggataa aaggcgggag gaagtcaaga ctgaggcaga gatccaggca
    10681 gaacggcagc aacgggaaaa acagcaggag gagcgccgca ggcagaacga agaatggcac
    10741 tggcagcgca gagagcggga aaagcagtgg gaagaacaac ggaaggctca ggcagagcag
    10801 aaccgtgcac ggaatcaggc acagccggtg acacccagaa aagctactgc ggcagaaatg
    10861 acttctctgg tcaaaatgga aaagaaggga aacgtgtccg gaacattgtg cactgtgttc
    10921 ggcgggatcg gactggggtt cagcggactg ggacttctgg tacagctgtt actggcagct
    10981 tttcaggcag catctctgag tgcggcaatc cttccgggag tattgtctct ggtattttta
    11041 gagatgatat cttatggcaa cggacagcgc agaagattaa agcgtgcgga gaaatatctg
    11101 gagctgtgcg gcaccaaaat gtatggtgtg atcaacgaaa tggcccgcta taccggaaaa
    11161 agtgtccgct atgtcagaag ggatcttcgt aagatgttaa aagccggaat gtttccggac
    11221 ggacatctgg atgacaaaga gacctgcttt atgctcaacg atgtgatcta ccgtcagtat
    11281 ctggagacgg agaaggctta tctggagcag aaaaagagca gtgagcagga aaaaaaacag
    11341 aatacagcgg acaccgacac acaggatacg gagcagagcc gcagagagca ggaactgcac
    11401 agtattgtgg cagaaggaat ggaatacatc cgtcatctgc gggatctgaa tgacaggatc
    11461 gaaggagaag cgatctctga gaagctgtac cagctggaaa atctgttgaa ggagatcttt
    11521 gaccgagtgc aggagcatcc ggagcagatg caccggatgc ataaactgat ggattattac
    11581 ctgcctacta tgttgaaact ggtggagcag tatgcggaat ttgacgaaat cagcagcccg
    11641 ggagaagata ttctcgatgc taaaaaagag atcgaaaaga cactggatat gatcaatcag
    11701 gcattcgtta cattgctgaa caatctgttc cgggatgcag cctttgatgc gtcagcggat
    11761 gcacaggtat tgcagacgat gttggctaag gaagggctga cggaatcgga ttttgtaaga
    11821 gaaaaaagtg tatgacagca ggaaagtaat gctgatggta aataaaaaat catgagagag
    11881 tatggaaagg cggggttatt tgtatgaagt tggagctgga tgctgcaact aaagaggcac
    11941 cggcccgtg
//
LOCUS       DAWDHD010000071        11696 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_348_length_11696_cov_10.090027, whole genome shotgun sequence.
ACCESSION   DAWDHD010000071 DAWDHD010000000
VERSION     DAWDHD010000071.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 11696)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11696)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11696
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_348_length_11696_cov_10.090027"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2979 a         3059 c         2548 g         3110 t
ORIGIN      
        1 tcctctttgt cccagaagca catgatcacc ttgccctgat gtcttgcaca gtcgacaaca
       61 tccgatacta ccgcgctggc ggtgggaagc ttacctgcgc cccgtccgta atacatggaa
      121 tctcccagca tgttgccggt gacaaataca gcattgaata caccatgtac catggcaagc
      181 ggatgttctt tggaaagtaa aaacggtgct accataacct ctgcggtatt atcatcaatg
      241 gccctgcttc tgcccaatag cttgatggta tgatctgtgg cattggcata ttcgaaatcc
      301 gctgctgtga tcttagtgat accttcggtg ggaaccttct cggaatccac atttttactg
      361 aacatcaggg aagacaaaat tgcaatctta cggcaggcat cataaccttc cacatccgct
      421 tccggattgc gctccgcata gcctttatcc tgcgcttcct tcagaaccgc agcaaagtcc
      481 gcaccctctt tttccatttt acttaagata taattcgtag taccgtttaa gataccgttg
      541 atagcctcga tcttctctgc tgtcagggaa taattcaacg gacggataat gggaataccg
      601 ccgcccacac tggcttcgaa cagatagttg caattatgtt ctctggcgat ctgcagcagt
      661 tcgggaccgt gcttggctac cagctccttg ttggaggtgc agacgctctt gccccgttcc
      721 agagcctgct tcgtgaactg ataagcggct ccgacaccgc ccatggtctc acagacaatg
      781 gtcacactgt cgtcatgtaa gattgtattg aaatcatgaa tgactttgtt ctcgtaagga
      841 tctccgggga aatctctcag atccaggatg tattttacct tcagttcctg tgcggacttc
      901 ttgttcacca tgtccttgtt tttctcgata acttccacca caccgctgcc cacggtgccg
      961 tagcctaata ctgcaacgtt gatcatatca taacccttgc ctttctcata gcctgcaaac
     1021 tttcaggtac tttagtacct tgcgcaggca caaatttgcg tgtgaaaaat tttattcttt
     1081 tgccagtatt tttacatgat gtacactggg ctgtccctcc agctgctcaa tcatccggga
     1141 aatgtccccc gtggtctgca atacctgaat actcaatgtc agagaagcga ttccgttgat
     1201 gggaatactc tgatggatcg tcaggatatt ggcccggtac tctgcgatga tcttcagcac
     1261 atccgacaat atccccggtt catcatccat ctgaaaagta agagtgatgg tcgttccctg
     1321 ggaattgtca tgaaagggaa aaatatcatc tttgtactta taaaaagaac tgcggctgat
     1381 acctaccgca tcaaccgctt cctgtatggt cagaaccttc tctgactcca gaagccgctt
     1441 tgcttccact acctttaaca gtacttcggg gatcgccttc tgcttcacca caaaatactt
     1501 ggtcgtttct cccatatgtt atcctttctt ccagttcgta tctgtagtgc ggacacttgt
     1561 ttttcactga gggatattct agcacagtaa agtatgaaag acaacccccc tttttatctt
     1621 ttttgagcgt caccgacaca catatccggc attttcagga ggttttgagt gattttaagc
     1681 ataagaaaag agccgaaagg ctcttttccc atccgctgtc tttagatcag aggatatttg
     1741 tctgttaatt tctgaatgat ggctctggct gcgggaacct gttcctcgcc gcccttgatg
     1801 accatggcaa tggcctctgc cacctgatcc atatcctcgg tgttcagacc tctggaagtt
     1861 accgcagggg ttcccagacg gataccactg gtcacgaacg gagactgggg atcgttgggg
     1921 atggtgttct tgttggcggt gatgtgagct gcatccaaca gcttctccac tgccttaccg
     1981 gtcagaccgt aaggggtcag gtctaccagc atcagatgat tgtcggtacc gccggagaca
     2041 atcttaatgt cacggctcat caggcccttg cacaatgcct gtgcgttgtc cacaatgttc
     2101 ttctgatata ccttaaagtc gtcgcttaat gcttcctcga agcagacagc cttggctgcg
     2161 atgacatgca tcagcgggcc gccctgaatg ccggggaaaa tagctttatt aaaattgaat
     2221 ttctcgttgg cttccttgtt gcagaggatc ataccgcctc tgggtccgcg cagtgtcttg
     2281 tgggtggtgg tggtcactac atccgcatag ggaatgggac tgggatgaag gcctgctgcc
     2341 acaaggcctg cgatatgtgc catatctacc atcagtaccg caccgacttc atccgctact
     2401 tcacggaact ttttgaaatc aatggtacgt gcatatgcag aagcacctgc aatgatcatc
     2461 ttgggtctgc attccagggc cagctctctc attctgtcat aatccaggaa accgttgtca
     2521 tccacgccgt aaggtactac atggaaatat ttaccggaca tattgacggg tgaaccatgg
     2581 gtcagatgtc cgccgtgatc cagattcata cccataatgg tatcgccggg cttcagcaca
     2641 gcgaactgca ctgccatatt tgcctgtgct ccggaatggg gctgtacatt ggcataatca
     2701 catccaaaca gcttcttggc acgttcgatc gccagattct cgatgacatc cacgcactca
     2761 caaccaccgt aatatctctt gccgggatat ccttccgcat atttattggt cagtggactt
     2821 cccatagctg ccataacagc cttacttacc cagttttcag atgcgatcag ctcgatgtga
     2881 ctgttctgac gattctcctc gtcaataatg gcctgtgcca cttcgggatc tacttttttt
     2941 acttcatcca atgaatacat gcctcattcc tccttatgtt acgttaattc gctaaactta
     3001 tggtttagta tagcatagaa ccttttattt gcaaagatag aaaatgaaca atgttggcag
     3061 aagccccctg gtgtgttaga ataaacttta acataactat tcagagcctc attcgccata
     3121 aaaattttca aaattatgct cattcatgca agcatgattc gcaaatttat gcaaattttc
     3181 ccggctctga atagttacaa ctttacgaaa gatggggaat tctcatgtat catattatta
     3241 tcaatcccgc ctcccgctcc ggaagagggg caaagatctg ggccgaacag gtggagccga
     3301 ctctgaaagc gggcaatacc gaatatcaga cctatttttc cgggaaagcc ggagaagtcc
     3361 gacagctggc cgcacagatc accgcagagc atgctgcaga gcctgactta acgctcatcg
     3421 ttctgggggg tgacggcacg ataaacgaga cccttcaggg cattgcagat ccttccaaag
     3481 tcatcctcgg ctacattccc accggctcca gcaatgatct ggcccgagat ctgggaattt
     3541 ccaaggatcc aaaggctgct ctcaatctga tccttcataa taatactccc agagccatgg
     3601 atctggggag actgacctat ctggacgaag accagccgga agaatccagg ctttttgccg
     3661 tcagctgcgg gatcggcttt gatgccgccg tctgtgcgga agccatgagt tcccggatca
     3721 aggataccat gaaccggatc ggcttaggaa agctgaccta tctgggtatt gccttaaaac
     3781 agctgatcac tgcccgcaag gtctcctgca cgctgacact ggaggacaag gtacatgaca
     3841 aaaaggaatc cgtagaactc ccccgttttc tgttcgtgac ctgtatgagt caccgttacg
     3901 agggcggcgg tttcatgttc tgccctcccg ctgtcgatca tgacggggta ttggatctct
     3961 gttccgtagg caatatttcc aagggattgg ttctgctggc cctgcccact gcctttttcg
     4021 gcaaacatta ttttgtcaaa ggcatcaatg cccataccgc cacacagatg tccataacag
     4081 cttccgctcc tctgtgggtg catacagacg gagaagtgac aagaaagagc cgcggtttcc
     4141 gggtggaatg tctccccggt gccatccgga tgatcacacc ttaaacagat ttaacacact
     4201 ctaaagattt tcttaagaat gcttaagtat tccaaaaaag ccttgatttt tttgttccga
     4261 tagctatact aaaattgatt ttagaacata ggtttttaga gaaatgaggt ttttatggaa
     4321 aaattacgga tcttctattt gaaatataaa catgccattc ctcttattat atatggcatt
     4381 atttacatgg cctggttcgc ttggttggag aaaaatacta ccggacatta ccggatcata
     4441 catatggcgg cggatgatta tattcctttt tgtgaagtgt tcgtcatccc ttactttttg
     4501 tggtttgcct atgtatcggc tgtggtcatt tatttctttt tcaagaacaa agaggattat
     4561 taccgcacct gtatcttcct cttcacgggc atgacgattt tcctgatcgt atccaccctg
     4621 tggcccaacg gacagcacct tcgtcccgct gttatgccga gagacaatat cttcacccgt
     4681 atggtagccg ctctgtatcg gacagacact cctaccaacc tgtggcccag cattcatgta
     4741 tataattctc tgggtgcaca ctttgccatt atccgcagca aatgctttga gaagaaaaaa
     4801 ggcatccgca tcggttccct gatcctggct gtctccatta ttatggcaac catgttcatc
     4861 aaacagcatt ccgtatttga tgtgcttact gctttcgtca tggcggctgt gatgtacacg
     4921 ttggtatatc gttatgatct gctgatcgct gtcagagagt tccgcaataa gagaaaagca
     4981 aaaccgcaga tcggctaaag gttgtttcaa aacatgcgcc gacggcgcaa ctataaaaat
     5041 gcagtgacgt tacccacgcc actgcatttc ttttgccctc atttatgatc tgttacatta
     5101 caatacttct tcgctcttcc tcaatgccga ggcaatgacc gcatccatat catagtagcg
     5161 gtattccgcc agtcttccgc cgaagatcac gttcttttcc gtatctgcca gccttttata
     5221 ctctgcatat aaagcgccgt tcttttcatc attgactgga taataaggct catctcccgg
     5281 cttccattcg gaactgtact ctctgctgat gaccgtcttc gggagatcct gtccctgttc
     5341 gtccttgcca aactcgaacc atttgtgctc gatgatccgg gtccagggag tctcacgatc
     5401 cgtgtaattc accgcagcat ttccctggaa gttgggaata tccagcacct ctgtctcgaa
     5461 cctgacggaa cgatattcca gtgctcccag agagtagccg aaataggcat cgatggggcc
     5521 ggtatagaca acagtctctg ccagtttatc cagttcctct ttctgctcca gataatctgt
     5581 gttcaggcgt acctcaatgc cctctaacag atgttccacc aacttcgtgt aaccgccaat
     5641 gggaatgccc tgatacaacg cattgaaata gttgttgtca aaagtcagac gcaccggcag
     5701 acgtttgata atgaaggccg gaagatcttt acagtcacgt ccccactgct tctcggtgta
     5761 gccctttacc agtttttcaa aaatatcctt acccaccaga gagatggcct gttcctccag
     5821 atttttcggt tccgtgatcc cggcagcctg ccgctgctct tcaatcttgg ccgcagcttc
     5881 ttcgggagtc accacgcccc acattttatt aaaagtatac atgttaaaag gcagtgagta
     5941 caactctccg tgataattgg ccaccggaga attggtgaaa cggttgaact ccgcaaatcg
     6001 cgtaatgtat ttccacacct ttgtatcatt ggtatgaaaa atatgggcac cgtatttgtg
     6061 gacatgaatt tcttccacgg tttccgtata aatgtttccc gccacattag ggcgtttatc
     6121 gatgaccaga acctttttcc ctctgtcagc cgcttctctt gcgaacaccg ctccgtacaa
     6181 tccggagccg accaccagat agtcgtattt cataatttat atatatttcc tttctcacag
     6241 tcgcattcct gcgcttacgt tatttccgta cgacagcctt ctctccgaag acacgtttca
     6301 gaaccaccag agtagccagt gtcagcagaa taccgatcac cagacagatc accacattct
     6361 gtacaaaacc ggcaatggta taagtcacaa aagagattgc cgccacggtc agtacataag
     6421 gtacctgggt ggatacatgg ttgatatgat tacactgtgc tcccgcagaa gccataatag
     6481 tcgtatcgga gatgggggaa caatggtcac ccataacagc acctgccaga catgcagaca
     6541 caccgatgat gatcagatgc tcggctccgg gtacgatagc tccggtcgtg ggatcggtaa
     6601 atacgccggt aacgataggg atcagaatac cgaaggttcc ccagctggta ccggaagcaa
     6661 aggccaggaa acatgctacc aggaaaatga tggccggaag catattggta agccccgtgg
     6721 atgctccctg catcaggcct gccacataga catccgcacc taacagtccg gtcatggtct
     6781 tcagagtaac ggcaaaggtc aggatcagca tggcaggaac catggcaatg aagccctggg
     6841 tcagacagtt catggcctcc ttgaaggata ccagtcttct gcacatatag tatacaatgg
     6901 caaataataa tgcgatcaaa gatccccagg gcagtcccac ggaagcatcc gttgcgctga
     6961 atgcatcgat aaagcccacg ccttcaaaga agccgccgac atagatcatg ccgatgacac
     7021 agcagacaat cagtacgatc accgggatca acagatcgta gaccttgcct ctggtggaac
     7081 ctttgggtgc attgtctgca ttggccacgc gctctccctc ggtgaacaga tcaccctttt
     7141 ctctggcatt gcgctcatgc tttgccatgg ggccgtagtc gaagcccatc agcgccatac
     7201 cgacgacgaa tacgatggtc agcagagaat aatagttata aggaatagct cttacgaaca
     7261 gttcaatacc ggaataaaca ccttcatcca ccataccgga tactgcggcc gcccaggaag
     7321 agatcggtgc gatcatacat atgggtgctg cggtggcatc aataatgtat gccagctttg
     7381 ctcgggaaat cttatgtccg tctgttaccg gcatcataac agagcctaca gtcagacagt
     7441 taaaatagtc atccacgaaa atcagaacac ctaataaaaa ggtggaaagc agcgctcccg
     7501 ctctggtctt tacatgcttt ttcgcccatt cgccgaaggc tgcagagcct cctgccttgt
     7561 tcatcagtgc taccatggta cccaacagga tcaggaacat gaagataccg atattccatg
     7621 catccgccac ggaattgata aatccttcgt tgatcaccgt atccagcgtt gcaataggag
     7681 aaaatccggt ataaatcaaa gctcccgtaa caatgccgat aaacagtgac gaatatactt
     7741 cttttgtgat cagtgccaat acgattgcca ccacaggcgg cagcaatgcc caaattgttc
     7801 cgtacataat tttgccctct ctcgtacttt ttttgtaaca tttcagatac atttttactt
     7861 tatggcaaac ccgtatacct gtcaatataa ttttccaaat taacgcttta gcacagcaat
     7921 ttatccgttc cttttctctt tttcgcttta aatcgctaaa ttttcttgca tccttgcacg
     7981 atctgggcta taatgagcaa tgacatttag cagaaaggac gtgccctgcg cacgatggaa
     8041 taccatgagt ttcgaattta tcaaaaaact gcccactcct gcggagatcc gcaaggaact
     8101 gccccttccg gcagaattag ccaggatcaa agaagaaaga gatgctgaaa tccgtgacgt
     8161 cctcaccggc aaaagcaata agtttctggt gattatcgga ccctgctccg ctgataacga
     8221 agattccgta tgtgattacg tcaatcgtct tgccaaagtg aatgagaaag ttaaggacaa
     8281 gctgatcctg gtgcccagaa tctacaccaa caagccccgc accaccggcg aaggctacaa
     8341 gggtatcacc agccagcccg atcccgagaa gaaaccggat ttcctggcag gtctgatggc
     8401 catgcgtaaa atgcacatcc gcgccatcaa agagaccggt ctcactgccg cagatgaaat
     8461 gttatacccg gagaactggg gttatgtatc cgatatcctc tcctatgtgg ccatcggtgc
     8521 ccgttccgta gaggatcagc agcatcgtct gaccgtcagc ggcttcgatg tagctgccgg
     8581 tatgaagaat cctaccagcg gagatttctc cgtaatgctg aactccgtat atgcggcaca
     8641 gcacccccac tccttcatct ataccggatg ggaggtgaat acccacggca acgatctggc
     8701 acacaccgta ctacgcggtg ctaccaacaa gcacggtgcc aatatcccca actaccatta
     8761 cgaggatctg atccgtctgt gggatatgta tgaaaaaatg gatctgaaga atcctgcctg
     8821 cgtgatcgat gcgaaccact ccaactccaa caagcagttc aaggagcaga tccgtatcac
     8881 caaggaagtc atgcacagcc gtaaactcag ccccgatctg cacaagcttg tcaagggcgt
     8941 catgatcgag agctacatcg aggaaggcaa ccagaaggtg ggtgccggct gctacggcaa
     9001 gtccatcacc gatccctgtc tgggctggga agacagtgag cgtctgatct acgatatcgc
     9061 tgagtacgaa taaaaagaac gattttgcaa atggggtgct gaatagttaa aacagtttca
     9121 aaaaaactgc aggaatcctt ttaataacgc aggattcaca aaacattcca ataaacatcc
     9181 gaaaaccagt gcgatccata tgagcgccag ttttcccaat ctgccgggca gggttttttt
     9241 tccctgcccg gttgtgtgat ggtaaaaata aatactccgg tgcatatctt cacaccatct
     9301 gagtaacagg atcatagccg gcaggtaaca cagataatga ggaaagatgc tcacgatccc
     9361 cagaaggatc cccttgattc catacctaaa cactgccgta ctcagaaaaa agcccacaga
     9421 gaatccgtat tttacggtga ctcctccgca gaaaatccat cccagataag tcgttgccat
     9481 gataataagc accagaaaat tccgacatct tttgcacagc acgtaccaga acaggatact
     9541 gctgtccacg gtcatatatt tcatgcgata taacgtgtcc tcatccagca ttcccgcacg
     9601 gtttaacaga attccccgtc cgaaatacac cgcaaacaat cccaccacaa atcccgtcaa
     9661 aaacaaaaag tgcagcggga gttttcccgc aggcaacatg gatctgttcc atttcatata
     9721 ttttcgtctc ccgtcaggcg ttgttagtaa tatatgcgga caacatggaa aatcatgctt
     9781 tcttattcgt ctttcagaaa agaaaaagcc catccggaca ggtactgcct gtctggatgg
     9841 ggttccctaa aatcattatg tagcagccgg tcgtgactgc ttttgtctgt aacggaatta
     9901 ctcactcagg tatttggatg cgtaagttaa aatgccgcag attgtcttgg catcctggat
     9961 cctgcagtcg tagatcatct gctttaattc ttctacggaa taagcctcta cattgatgaa
    10021 ttcatcttca tccagatgct ggtttcctct gtctttcagt cctctggcca gataaatatc
    10081 gatcttctca ttgcagaatg ccactgtggt ataaatagag gtcaacagtt ccacatggtc
    10141 agtatgatac ccggtctctt cctgcaattc ccgcatagct gcctgatccg tgggctcttc
    10201 tctcccgttc agtccgcctg ccgggatctc taacgtctca cgctccaggg cattgcggta
    10261 ctgacgcacc atcagaagct taccgtcttc tctcacggct actactgcag ccgccccttt
    10321 atggtcgatc agatcccatt ttgctatatt accgttggga atctgcatgg tatcctgata
    10381 atagtcgata atagcaccct ttgctaccag atcacggctg atacgcttga attcttccat
    10441 aacttatcat tccttctcat ccaaatcgaa tacttacctg ttttccagta atttatcaac
    10501 gctcaccatc ttgacgcaga gtacgaacag atccgtagcc attgccatct cctccggtgt
    10561 ggggaaggaa ggcgcaatac ggatattgga gtccttggga tccttcttgt aggggaaagt
    10621 agcgcctgct ccggtcagag tgacgcctgc ttccttacac tttgccacaa tggctttggc
    10681 gcagccttcc atggcatcaa aagaaataaa gtaaccgccg ttgggcttag tccaggtacc
    10741 gatgcctaag cctcccaact cattctccag cacttgcagt acagcctcga acttgggacg
    10801 catgatcgct gcgtgcttct tcatatgttc acgtacacca ttgatatcct taaaataacg
    10861 tacatggcgt aactggttga tcttatcata accaatcgtc tggatcgtca tggacttgcg
    10921 catatcatcc aggtttgcct tggaagatgc aatagccgca atgccggctc ctgcgaaggt
    10981 taccttggag gtggagcaga actcgaatac catgtccgga tgtcctgcct tttcacactc
    11041 ggaaaggatc tctaacagct gatcctgtct gtcatcatat aaatgatgga ttgcataagc
    11101 attatcccaa aaaatacgga aatccggtgc agcaggctgc aatgcagcga aacgtcttac
    11161 ggtctcatcg gagtaggaat atccctgagg gttggagtat ttcggtacgc accagatacc
    11221 cttgatagcg ggatcttcag acaccagttt ctctaccaga tccatatccg gtccttcggg
    11281 tgtcatgggc acattgatca tttcgattcc gaaatgctct gtgattccaa aatgtctgtc
    11341 atatccgggt acgggacaca ggaactttac atgatccagc ttacaccagg gggtattgcc
    11401 taagatgcca tgggtcatag cccgggatac ggtatcatac atgatggtca ggctggcatt
    11461 gccacaaacg atcacatttt ccggcttcac ttccagaatg tcagcgatca gtttctttgc
    11521 ctcaggaata ccgtccaggc caccataatt tcgaacatcc attccatcct cagccttcat
    11581 agcgctctga gaatttacaa catcgagata atccattgtc atatccagct ggttaactgc
    11641 cggttttcct ctggacatat ccagtttcag gcctttgcct tttgcatctt catatg
//
LOCUS       DAWDHD010000072        11658 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_350_length_11658_cov_12.052917, whole genome shotgun sequence.
ACCESSION   DAWDHD010000072 DAWDHD010000000
VERSION     DAWDHD010000072.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 11658)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11658)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11658
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_350_length_11658_cov_12.052917"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2691 a         3144 c         2530 g         3293 t
ORIGIN      
        1 gtcttatagg cctccatatt ggggaactgg atccaggctc ccgccggcat gcctattttc
       61 tgaggggttc catttgcctg tgtacgctgc ccagactgca tttgtcctgc tgcctgaccg
      121 gtctgtccca ccgacatact actgctgttc ataccgtcat tttggtattg tccgtagccg
      181 ttcccggcat tccggtatct accggcaccg ccatttccac ttccgtttcc gtaggatccg
      241 ctgcggctgc tgccgccccg gcctccgtaa tttctgggga acaggggctg gttcgccgct
      301 ctcgcagcct gcgcatcctc gaaactgatg atctggtcgc agatcagctt gccgtccttg
      361 tcttcttcca gggatactct gccccggata aacaccttcg catcctccac aagcaaggtg
      421 ctgtatcgct cgtacacctg tggaaatacc acgacttcca cattaccgat cagatcctcc
      481 acattcaaaa atgccatgat cttgtcattc ttggtgtatt ttactgtctt gtccgcgatc
      541 aatcctccga tgactgcatt ggcctggtcc ctgactaact gcacctctcc ggtctcttca
      601 tccagtgcaa aatccccggc agtattgctg atgcagttct gccacagctc ctgatattcc
      661 tccaggggat gaccgctgac gtagacaccc agcacctctt tttcgaagcc cagcagcatc
      721 tccttattgt attcgcccac atccggcatc cggatatcga attcttcctt atcctcttcc
      781 cctgcaatgt caaacaggga gatctgtccg gccagattgt tctttttatc atgggcaata
      841 tggtccgcaa tctgcacata gacactcatg aactgttttc tcgtaccgcc cagaccatcc
      901 agagctcctg ctttgatcag gttctcaata gcccgcttat tcatctcttt tttatcggac
      961 atgcgggtaa taaagtcctt cagattcgtg aaagggcctc tttccttacg ctcctgtacc
     1021 aggctgtcaa tgatgggacg tcccacactc ttgatggccg tcagggcata acggatcttg
     1081 ccatccgata cggagaatcc ggcttctccc gcattcacat ccggaggtag aatggcaata
     1141 tccatgttcc ggcaattcaa aatatattcg gatacctttt tcgggttatc gatgacggag
     1201 gtcatcagcg ccgccatgaa ctccacggga taataatatt tcaaataagc cgtctggtaa
     1261 gccaccactg cataacaggc cgcatgggac ttgttgaacg catatttggc gaaatccatc
     1321 atcatatcgt agatctgtcc ggctgtctgc tcgtcaatgc cgttggcaat acagccgggg
     1381 acattttcct ccgggttgcc gtagatgaag ttggcccgct ctttttccat gacggactgt
     1441 ttcttcttac tcatggcacg gcgcaccaga tcgcttcgtc ccagtgtata gcccgccaga
     1501 tcacgaacga tctgcattac ctgttcctga tatacaatac agccgtaggt gggctttaag
     1561 atcggctcca gctggggaca ggcataagtt accgcatccg gatgattttt accctgaata
     1621 tacttcggga taaaatccat aggacccgga cgatacaggg aaataccagc caccacatct
     1681 tccagattct ggggacgcaa ttccttcatg aagctcttca tcccggcact ttccagctgg
     1741 aacactcctt ccgttctgcc ggtgccgatg gaagccagta ctttcggatc gttatagtca
     1801 atgacatcca catctatgtg tcttccggtg gtattctcaa taaattttac cgcatcatgg
     1861 atcacagtca gtgtacgcag ccccaggaaa tccattttca gcagtcccag ttcttccaat
     1921 gtcgtcatgg taaactgggt gacgatggaa ccgtcggagc ctctggacag cggcacgaat
     1981 tcatccgccg ccttctgaca gatgaccacg cccgccgcat gcatggaggt atgtctgggc
     2041 agtccttcca gacgcttaca catatcgatc agatgatgca cctgctcatc ctcctgataa
     2101 agctttcgga attccgggtt catttccagc gctttgctca gggtgatgtt cagttcgtta
     2161 ggcaccatct tggcaatgga atccacgaac gcatagggca gatccataac acgtcccaca
     2221 tcacggatga cacccttggc tgccagggta ccgaacgtaa cgatctgcac caccttgtcg
     2281 ttgccgtatt ttctgcccac ataatcgatg acttcctgtc tccgttcgaa gcagaagtcc
     2341 acatcaatat cgggcatgga cacacgttcc ggattcagga aacgttcaaa cagcagatta
     2401 taatgaatgg gatcaatgtt ggtgatcttc aggcaatatg ccacgatact tcccgcagcg
     2461 gaacctcgtc ccggtcccac cggaatcccg tgctctttcg catagttgat gaaatcccat
     2521 acgatcagga aatagtccac atatcccatt ctgcggatga cacccagctc gtaatccaga
     2581 cgttcccgca gggtctgtcc ggtctccccg gcaggctggt caccgtcacc gtaacgctct
     2641 gccagtccgt cattacaaag cttgttcaga tagctccagg aatcataacc ttccggcaca
     2701 tcgtatttcg gcagcttcgt cacgccaaac tctatctcca cattacaacg gtctgcaatc
     2761 ctctgggtat tctccacggc ctcccatgca taggggaaca gtcccttcat ttcctcttcg
     2821 ctcttgacgt aatactgacc gccctcataa cgcatgcgat cctcgtccgc cagctttttg
     2881 ccggtctgca gacacagcag aatatcgtgg ggaatcgcat cctcagcata ggtgtaatgc
     2941 acatcgttgg tggttaccag cggaatgtcc agttctctgc tcatctggag cagttccata
     3001 ttcacggtac gctgctccgg gataccgtga tcctgcagtt ccagaaaata attgcccttg
     3061 ccgaagcagg cctcgtattt gcgggctgct tttctcgctt cgtccttcat tcccttcaga
     3121 ataaaccggg gcacttcacc tgcaaggcag gcagacaatg cgataattcc ctcgtggaac
     3181 tcctgcagca cttccatgtc cacacggggt ttataataat atccttccgt aaagccacgg
     3241 ctgacgattt tcatgagatt cgcatagccg gtattgttct ccgccagcag caccagatga
     3301 tgataacgtt cttccccgcc ggtcagttcc ttgtcaaaac gggaattcgg agccacatac
     3361 acttcgcagc ccaggattgg cttgatcccc gcttctctgg ctgcccggta aaaatcgatc
     3421 acgccgtaca taacaccatg gtcggtgatt gccgcgctgt ccatgcccag ctccttgacc
     3481 ctggctacat actccctgat cttgttggag ccgtctaaca gcgaatattc tgtatgaaca
     3541 tgaagatgtg caaatgccat tttgtcgttc cctttctagt gcagttcttt tgcttttctg
     3601 acgcattcct cgtaagttgc tcccacctgt tccctgtggt agccgggagc cagcatcgcc
     3661 gcgaaataca aggtctccag tgtctcttcc tgtgccggtg tgggatggga aggcaccacc
     3721 acacgggtgc tccagatcag catacagccc gtatattcca ccatgaaaaa aatgggctcc
     3781 atatttttct ccatacggct gttcagtgtc agtttatctt cgccggagat ttcctccagc
     3841 tttttgatat gagaaggctg tggctcttcc acactgccgt tcggcaggat cacgcactcg
     3901 cagggatcca tatcctctct gtgtgcattg ataaattcca tgacagtcat tctcacacct
     3961 cctttgttcc gtctgtcagt ctgtctgttc ttccgcccgg accgcccaac gcatcttcgt
     4021 ggaacgggct ctgccgttct gtacacattc ctccgcagaa gggcggatca cgtctttcgc
     4081 ataatcggca taaatgccgt tccggtagcc ttccttcaat gcctgcttca ccagcttgtc
     4141 ttcgccggaa tggaaagtca ggatagctac tcttccgcca ggtttcaagg ctcccggcag
     4201 cttttccata aattcatata acacttcaaa ttcgcggttc acatcaatac gcaaggcctg
     4261 aaaagttctc tgacaggtct ttttgatggt atcctttttc tccttctccg ggaggaaatc
     4321 cagagtcttt tcgatgactt cccttaattt tgtagtagta tcgatccggt gtcctttacg
     4381 gatctcatcg gtgatggcct tcgccagttc ctcgcagtag ggttcgtcgg aattctcgta
     4441 aagcattccc gccagttctt ctctgctgat ggtatccagg cgctctgctg cactgattcc
     4501 ttgctgctga ttcagtctga gatctagcgg accgtccacc ttgaaggaaa atcctctgct
     4561 gggattatcg atctgcatgg aagatactcc cagatccgcc agaataaaat caaatcctcc
     4621 gacctctttt gcgatctcat ctatggtaca gaaattctgt aatctgatgg tcagaatctc
     4681 ttctccgtaa ccggcctctg ccagtcgttt cttcgtcttg gcagactcct ccggatctac
     4741 gtctgtggca tagatatgcc cacacccctg cagctgctcc aacatggctt tcgtatgtcc
     4801 accgtagccc agtgttgcat caaatccggt ctctcccggc tggatcttca ggaaatccag
     4861 gatctccttc accataatag agatatgcat gcctgcggga gtgtttccct tctgaatcac
     4921 atgggcaatg gtatctccgt atttttcggg ctgtaattcc ttatattttt cctcaaattt
     4981 tctggggtat ttccctttat aacgcacccg gcgcttgtgg ggtgtctgtt cctgatccat
     5041 tcccatgatt acctcatttc actcattttg ttccagttct gttccctgta attatatcgc
     5101 atatcagata tccttgtcca cttcctgtca gagaaaaaaa gcaggcatcg ttgccgatac
     5161 ctgcttttcc agctgtacta tatttttctc aatgtctcag ccgttcttat tcctgaggaa
     5221 gaataaactt gctgatcagc tcatccagag agattgcctg tgcagacaat tcctggctgg
     5281 tagcggaaga ttcttctgcg gtagcggagt tggactgtac tacctcggag atctggttga
     5341 caccctgctc tgcctgttcc atggcattgg cctggtcctt ggagatctcg ctgatactgc
     5401 gggaagactc tgcaatcttg ccgataccgt ctactacgat ctcgatggaa gcagcagcgc
     5461 tgtctgcagc ctggttgcct tccttgatct cctgcatgga accttcaatc aggtctctgg
     5521 tatctacagc agacttggta ctctgctctg ccagctgacg gatctggtca gcaaccacag
     5581 cgaatccacg gcctgcctca cctgctctgg ctgcctcgat ggaagcattc agggacaaca
     5641 ggttggtctg ggatgcaata tcctcgattt cagaaatgat attgccgatc ttgttggaag
     5701 tatcactgat tctggccata gcatccacca tagccttcat ctctgctctg ctgttatctg
     5761 cttcgtttgc atattttctg gcctgctcat aggactcctg tgcctgttca gcagcctgct
     5821 caatattgga agtaatgttg gtaatggtag cctgcagttc ttctacggca cctgcctgct
     5881 cggtagcgcc ctctgccaga ttctgggaag catctgccag gttagctgca cctgcggata
     5941 cctgcctgga agactcctcg atggaacgca gtgtctgaac catctgtgtc ttcatgttac
     6001 ggatagacag aaacaactgt tcaaaatcac cctgatacat atccgggttc ttggaagtga
     6061 tgttgtaatt gccgtttgcc atctggctca taacttctcc ggtgtcggca atgacaaaac
     6121 tcagcttttc tgccatttca tttgcagaat gaatcatgtc agcgatctca tccttactgt
     6181 tcagtgtcgg gaacggatcg gacaggttac cctgtgcaaa agtctccaga cgctttgcca
     6241 atgcatccag cggtgctgcg attccctgag agattgcatg ttccattctc gttgcaatga
     6301 taattgccag cacgatcaca ccaacaatga taagcaggaa gataaatccc atgatattca
     6361 gtatggaaga aagggaatct ccttcacgga cattctcgtt cataagttca cgcatggtat
     6421 cataaatttc attataaagc ggtgctacct gattctgcat cagctcctgt gcctgcatac
     6481 gcttctcttc catatccacc ttaccggtat taatggcctg ctgatccagc tcccagtact
     6541 gctgaatctg ctcatttacc tgatcataca gttccttctc ctttgaagaa acaacagtct
     6601 cgccgacaac ttcccagtat tcaagaaaag cctgcttctt ctcatcgtga gtcgtgatca
     6661 gaccgtccac cagatccggg tcgttatatc ctatgattcc gcgggttgcg gatcttacat
     6721 ccgtgaatac agtcatggcc ttaccgatat ctccctgtga aaaaccgtaa ttggtcaatg
     6781 catgctgata gcggttcatg gtaacgatca ttgccagtgc tccgagcact cctgcaatag
     6841 atgatataac cacaataatg gcaaaagctt ttttcaggcg ctgcctgatt ttcaaatttt
     6901 cgaacatttt cctctcctta ctattggttg ataacaagtc atattataat attgtaacat
     6961 tttgtaaaaa tatgtaaata acttttttaa tttcgattat atttttatat tttatttgtc
     7021 ttttccctgt tttccgtgtg tggcggatca atattctcca ttaaacgcat tcactgtata
     7081 cacagaaaac tctcctcaac agaataacat ctgtccggag agtttttcat agtaatagta
     7141 atataggaga ttataatgct tttttgatgg ctgccatcag ttcgtcatag gtctcatagg
     7201 ctggaagctc cggatgatct tttttgatct gttcggtact cttgaacagg aagcctgcct
     7261 tgctggcctg gatcatgccc agatcattgt ggctgtctcc actggcgatg gtctggaagc
     7321 cgatggactg cagcgccttc accgtagtat acttggaatt ctccacacgc atcttaaatc
     7381 cggtgatctc accattatcc gctacttcca gagaattaca gaaaattgtg ggatatccta
     7441 gtttcttcat cagaggaccg gcaaactgtg aaaaggtgtc actgataatg atgacctggg
     7501 taatggaacg cagctcatcc agaaactcct tcgccccggg gatggggtcg atctttgcga
     7561 tagtctcctg aatctccttc agtcccagac cgtgttcctt cagaatacca agtctccatt
     7621 tcattaattt atcgtaatcc ggctcgtctc tggtggttct cttcagttcc gggattccgc
     7681 tggcctccgc aaaagcgatc cagatctccg gcaccagtac tccctccaga tccaggcata
     7741 caatattcat atccatcctt atgtcctccc agtaaattca aatgtatttg tgacaagtat
     7801 accccatttt acctttttac gcaacctttt actttattgt tgtaacgtgt tcttgtaatg
     7861 cagttgtaaa tttttctcag atatatcatc atacgcacac aattttgaaa cgaatatttc
     7921 catgtgtttt acatattttc gtttcatgtc gtagatgcca aagagtgccg cttcacattg
     7981 ttttctgaaa tttgcttgta aatcccactt tttttctgtt atttttgtga cattttaact
     8041 gtttactttg ttctatatgt gttataataa ccatagtgca aaaagaactt ctacaagctc
     8101 tcatctacac gaaacaaacg tagagatcga ggaggtcatc attatgttga cattaaaagc
     8161 tgaaaaaaga aatcctgacg aaaaagcaaa gaaattaaga agagacggtt ttatcaccgg
     8221 tgttttgtac ggaaaagaaa tgaaagagaa tgtttccctg aaattcacag agaaggatgc
     8281 atccgctttt atcaagaatg caaaagaagg cacccaggct tatctggaac tggatggtaa
     8341 gaatgtagat gttctggtaa aaaatattga ttttgatcct ctgaccaaga aatatatggc
     8401 tctggacttt caggctctgg tagccggcga aatcgtatcc gctactgttc ccattgtcta
     8461 tgtgaacgaa gacaaggtac agggtatctt cgaagcagaa ttgtccgagg tgcattacaa
     8521 agcagatccc gcacatctgc tggagcccgt tgaaattgat ctggccactc tgccccagac
     8581 tacgaagacc atgtatgtca aggatctgaa gctggaagaa aaaggtgtgc atgtcactac
     8641 ctccggagaa cagcagctgt tccatatcgg agagtatgga caggaagaga cggacgagac
     8701 cgaggagaca gaggaaacca aataaaacgc catgcttcat tcatacatgt gccgcataaa
     8761 aatcaaaaca ggaactgttc cctgtgagta aactcccggg aacagttcct gtttcatttt
     8821 tgcatggctc gtaactatgc caggtatttc ttcagtaaat ccaccttgtc cagcttctcc
     8881 cagggcagat ccacatcggt acgtccaaag tggccatagg aagcggtctg cttgtagata
     8941 ggtctgcgca gatccagcat cttgatgatg cctgcgggtc ttaagtcgaa gttctcacga
     9001 atgatctcca ccagcttatc atcatgcagt ttgcccgtac cgaaagtatc taccatgatg
     9061 gaagtaggat gtgccacacc gatagcgtaa gacagctgga tctcacactt ttcagccagt
     9121 cctgccgcta cgatattctt ggcaacgtaa cgtgctgcgt atgccgcact tctgtccacc
     9181 ttggtgcagt ccttgccgga gaatgctccg ccgccgtgac gtgcatatcc accgtaggtg
     9241 tctacgatga tcttacggcc ggtcagtccg ctgtcgccgt taggtccgcc gattacgaaa
     9301 cgtccggtag gattgatgaa gaacttggtg tctgcatcga tcatgtctgc gggcaaaaca
     9361 ggatcgaata catacttctt gatgtcctca tggatctgct cctgagatac atgttcgtca
     9421 tgctgggtag acagaacgac tgcctccaga cgaaaaggct tgccattctc atcatactct
     9481 acggatacct gactctttcc gtcgggtctt aaataggaaa gtgtaccatc cttacgaacc
     9541 ttggtcagct gtctggtcag cttgtgtgcc agagagatgg gatacggcat atattccgga
     9601 gtctcatccg tagcatagcc gaacatcata ccctgatctc cggcaccgat ggcatccagc
     9661 tgttcatcgg tcatgtggct ttcttttgct tccagggcct tgtctacgcc catggcgata
     9721 tcagtggact gcttgtccag agataccata acggcacagg tgttgccatc gaatcccttt
     9781 tcggaagaat catagccgat ctcggtcacg gtcttacgaa caatagcggg aatatcaata
     9841 tttgccttgg tggtgatctc acccattacc agtacgaaac cggtattggt gcaggtctca
     9901 catgctacac ggctgaacgg atcctgctcc atcagcgcat ccagtaccgc atcagatacg
     9961 gcatcacaga ttttgtcggg atgtccttcg gttacggact ccgatgtaaa taaacgtttt
    10021 tccatttgtc ctccattctg cttccttgcg ggaagagggt ctagtgtatg agttgctaaa
    10081 gtcctgtcat tcagagcctc attcgcaaaa tcaaattttc aatgccgaca gctctgaatg
    10141 atgcaaaaaa gtccgctttc ataagcggac tgatttattg tgcgatcaaa tcctcttatt
    10201 gttcgacagt tattcgctca ggtgggcacc ttccgcattc tgcggggttg ccgtggcttc
    10261 atcgatccta tgtatctcca ccactctgaa taagggaaag tctatatgaa cttttctacg
    10321 ctaattaact tactatgagt tacctgtttt gtcaacagac atttttttca aaatgtcatt
    10381 ctgttgtggt ttatgagaaa tccactcatt aaccgatctt cagcttgaac ttctgcaggt
    10441 cccgctccgt ctcgaccaac tcgatctggg ctcccagtcc ctgtaatttc aggtggaaat
    10501 cctcataacc tctctcaata tagcggatat catccacagt agagaatcct tctgcagaca
    10561 tggcagccag taccagtgct gctcccgccc gcagatccgg tgcggaaata ttggctccgg
    10621 tatatctgga tacaccgtcg atgatagcgg tgttgccttc tactttgatg ctggcgccca
    10681 tacgggtcag ctcatccaca tatttgaaac ggttctcgaa aatactctcc gtcacgatgc
    10741 tggttccttt ggaaagtccc agcgccactg tgatctgggg ctgcatatcc gtaggaaatc
    10801 cgggataagg cagggtcttc acctgggtat gctgcagagg cttggacgct accacacgga
    10861 tacagtcatc cgcttcctct acttcacagc caatctccag aagttttgcc gtaatagatt
    10921 ccaggtgctt cggaatcaca tttttcaccg tcacatcgcc cttggtcact gccgctgcga
    10981 acataaacgt accggcttcg atctgatcgg gaatcacggc atactctgtg ccatgcagat
    11041 gggaaacacc cttgatacgg atcacatccg taccggcacc cttgatattg gctcccatgc
    11101 tgttcaggaa gttcgccaga tccaccacat gaggctcctt ggcagagttc tcgataatcg
    11161 tggtgccttc cgccattacc gcagccatca tgatattgat ggtagcgccg acactgacca
    11221 catccatata aatatggctg ccgcgcagat gctccgcctg tgtcttgatc aggccatgct
    11281 cgatcttcac gtccgcgccc agagcacgga agcccttaat atgctggtcg atggcacggc
    11341 agccgatatc acatccaccg ggaagtgcca cttccgcatg cttgtattta cctaacaaag
    11401 caccgatcag atagtaggac gctctgatct tcttgatatt ttcatcttct actacaagtt
    11461 cactgatgtt ggatgcattg agagtcactt tgtgacggtc ggtcctcttt acgatcacgc
    11521 cgatgttctc catggcgctc aaaaggacat tcgtatctct tacatccggc atattttcta
    11581 tataaacagt ctcatccgtc atcacggaag cagcaagaat aggaagtgcc gcattcttgg
    11641 cacctcctat ttcaactt
//
LOCUS       DAWDHD010000073        11556 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_353_length_11556_cov_9.484740, whole genome shotgun sequence.
ACCESSION   DAWDHD010000073 DAWDHD010000000
VERSION     DAWDHD010000073.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 11556)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11556)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11556
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_353_length_11556_cov_9.484740"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3198 a         2382 c         3010 g         2966 t
ORIGIN      
        1 aactgctatc atgatatttc cgagctgccc agtcatgtgg tggacgagat ggagcatttc
       61 ttcacagtat ataaggcact ggagaacaag accactgctg tcaatgaaaa gggcgacaga
      121 gatgaggcaa ttgaagtcat taaaaagtgt ctggacaatt acattgatac ctttatcaag
      181 agttggtaag ctgttgataa ttgattttca acaatcacaa agcaccgtat ttcccatgca
      241 gttcattggg ggatacggtg tttatttcta taaactattg aaaaaaattg tcaaaacccc
      301 tagatttttt aaaggaaata tgagagaatg aacacgacca ttatgatatt attttatcaa
      361 tagtattata agtacatatt gaaaggagat ttcacatgat ttattcaaaa gaagttgaag
      421 aaatgtgtcc cgttgcacag ggcgtacatc atggttgtgc tcccattccc gaagaggcaa
      481 aatgggtgca ggcaaaagaa gttaaggata tttccggtct gactcacggt gtgggctggt
      541 gtgcacctca gcagggtgca tgtaaactga ccctgaacgt taaggaaggt atcatccagg
      601 aggctctggt tgagaccgtt ggatgttccg gtatgactca ctccgcagct atggctgctg
      661 aaatcctgcc cggtctgacc gttatggaag ctttaaatac tgaccttgta tgtgatgcaa
      721 tcaataccgc tatgagagag ctgttcttac agatcgttta tggtcgtacc cagagtgcat
      781 tctctgagga aggtctgcct gtaggcgcag gtctggaaga ccttggtaag ggcttaagaa
      841 gccaggttgg tactatgtac ggtactctga agaagggtcc ccgttatctg gagatggcag
      901 aaggttatgt aaccggtatc gctctggaca aggatgatca gatcatcggt taccagtttg
      961 ttaacctggg caagatgacc gacttcatca agaagggtga cgatgccaac actgcatggg
     1021 agaaggctaa gggccagtat ggtcgtgtag acgatgcagt taagatcatc gatcccagaa
     1081 aacaataaga tacaggagga cagaaaaaat ggctttattt gaatcatatg aaagacgtat
     1141 tgataagatc aacgctgtac tgaacagcta tggtatctcc tctatcgaag aagcagagaa
     1201 gatcacaaaa gatgcaggcc tggatgtata cgatcaggta aagaaaatcc agcccatctg
     1261 ttttgaaaat gcttgctggg cttacactgt aggcgctgca attgcaatca agaaaggctg
     1321 cagaaaggca tctgaagcag ctgcagctat cggcgaaggt ttacaggcat tctgtatccc
     1381 cggttccgtt gctgataccc gtaaggtagg tcttggccat ggtaacctgg gcaagatgct
     1441 gctggaagag gatactgact gtttctgttt cctggcagga catgagtctt tcgcagcagc
     1501 agaaggtgct atcggtatcg cagagaaggc taacaaagtt cgtcagaagc ctttacgtgt
     1561 tatcctgaac ggtcttggta aggatgctgc aaagattatt tcccgtatca acggatttac
     1621 ctttgttgag acctcttatg atccttacac caacaccgtt accgaagtag agagaatcgc
     1681 ttactccgag ggacttcgtg ctaaggttaa ctgctacggc gctaacagcg ttcccgaagg
     1741 cgtagctatc atgtggaagg agaacgtaga cgtatccatc accggtaact ctaccaaccc
     1801 taccagattc cagcatcccg tagctggtac ttacaagaag gagagactgg aagcaggcaa
     1861 gaagtacttc tccgtagctt ccggcggcgg taccggacgt actctgcatc ccgataacat
     1921 ggctgcaggt cccgcttctt acggtttcac cgatactctg ggccgtatgc actctgacgc
     1981 gcagttcgct ggttcttcct ccgtacctgc acacgttgag atgatgggtc tgatcggcat
     2041 gggcaacaac ccgatggtag gtgctaccgt agctgttgct gtatccatcg aggaagctgc
     2101 taaggctggt aagttctaaa acataaaatc agataaatca aggatttcaa ggctcccgac
     2161 attgttcggg agtctttttt tccacaaaat tactttgtaa tcgaacatgc attctgctat
     2221 aatcatttat agaaagtgtt tgaaaggagg cagcaatgga atcacaatcc catacttata
     2281 ttgccatgga tctgaagtct ttttatgcct ccgtggagtg cgttgacaga gggctggatc
     2341 ctttgaatac gaatctggtg gtggcggatg cttcccggac gcagaagacc atctgcctgg
     2401 cggtatctcc ctctctgaaa tcctatggaa tcccgggacg ggcaagactg ttcgaagtga
     2461 tctccaaagt aagagaagta aatgccatgc ggaaagtgcg gtcccctggc ggcagatttt
     2521 tgggaaaaag cacctttgcg gatgaattgc agaggcatcc ggaactggaa gttggttttg
     2581 tgaccgccac gccccggatg gcgagatatc tggaatatag tacccagatc tataatattt
     2641 atttgaaata tatcgcaccg gaagatattc atgtctattc tattgatgaa gtgtttctgg
     2701 atgtgacaca atacctgaag acctatggta tgacagcgag agaactagca tccagaattg
     2761 caacagagat tctggagaaa aaagggctga ccgccacagc aggaattgga accaatctgt
     2821 acctgtgcaa gattgccatg gatattgtag cgaagcatat ggaaccggat gataacggag
     2881 tccggattgc cgaattggat gagatcagct atcgggaact tttgtgggat cacaggccgc
     2941 tgacggattt ctggagagtc ggcgccggat accgcaagaa gctggaagcc gccggaatgt
     3001 ttaccatggg cgatgtggcc agatgctcta tcggaggact gggagattac tataacgaag
     3061 atttgctgta ccggatgttc ggcatcaatg cggagctttt gatcgatcct gcctgggggt
     3121 atgagccggt gaccatggac ctgatcaaat cctatcgacc ggagactaac tgtatcagct
     3181 ccggccaggt actgcattgc gccacggatt ttatgcaggc caagatcgta gtccgggaaa
     3241 tggcagaaca gctggcaatg gatctggtgg aaaaaaagct ggtcacagat cagatcgtgc
     3301 tgactgttgg ctatgatatt gacaacctga agattccgga gatcgcagat cattatcatg
     3361 gtgaaatcac ggtagatcgt tatggcagaa gcgtgccgaa gcatgcccac ggcactggaa
     3421 accttgatgc aatgactgca tcagtcagca ggattacaga gacaacaatg ggattatatg
     3481 aacggattgt agatcccatg ctgctgaccc gaagaatcac tctggtggcc aatcatctaa
     3541 aggatgcgga cagcgtaaag gaagagccgt tctacgaaca gatggatctg ttctccggag
     3601 gctttttgca aacggggcag aaagaaactt cccgggaaac aaagcagctg gaaaaggaac
     3661 ggctggaaaa agaaaaacac ttgcaggaag caatgctcag cgtaaaatca aagtatggaa
     3721 agaatgcgat tctgaaggct gccgatctgc aggagggagc cacaaccata gacaggaacc
     3781 gtcagattgg cggacataag gcataatatt tatcggacca caaaatccga taacaggaaa
     3841 agagtaaaaa gatgaatgaa aaatataagg acatgctcca tctgcctcac ccggtgtcag
     3901 ccacccatcc ccgaatggct ttacaggacc gtgctgcaca gttttcaccg tttgcagcat
     3961 tggcgggata tgatgacgct ctgagagaaa cggcccgccg gacagaccgt tttgtggaac
     4021 tggatgagga caggaagcag gagctcgacc gccagatttc ttatttgcaa cagcatctgc
     4081 tggacacagt gccggtgaaa attatttatt ttgtgccgga tgagaaaaag gacggcggca
     4141 gttatactgc cgtagaaggc tgtgtccgca aaatcgatga aaacacaaaa agccttcgga
     4201 tacagggaac agaaattccg gtagagagga tctacgggat cgattttctg tagttacatt
     4261 gtaaatagtt cagagaaatg ctaaaatatt accaatagta ttttgagaac aagaatggaa
     4321 aggaatttct gacaatgaag aaaaaatgta tcagtacagc aacactgtca gtgcttttgc
     4381 ttgtactgtt tcttcctgtt ttctattata tagtattcta cgggaccaat atgaactaca
     4441 acgagatgca taagatcatt accgtggagg gaaataaagt actatccctg tgcgcggtga
     4501 tcggagtggc tgtactgggg gcagcgtact attttctgcg aaagattcct tacaccggac
     4561 gcatagcagt ctggtttacc gggatcacac tggcagtctg tattctgttc tgtcttgtga
     4621 atataaaaat atccaaatgc attgcatttt acggtggctg ggattgcggc atggtggcca
     4681 attccgcccg ctggctttat gaaggccaga cactggggta tgacgattac tatacgatct
     4741 acagtaataa tattcccgtt acatggcttt tgtatcagtt gtattctttt gcctcaggac
     4801 ttaaggggta tccctacaat ccggaattta tctggatcca gttccagtgc gtcatgctgt
     4861 cactggcggt attctgttcg gtacttctgg tccttcaggt cagcaggaac cttggaattt
     4921 ccgtgattgc cctggtcttt cagagcatct ttctgggaat ctctccctgg aagatcattc
     4981 cctatacgga cggaagcacc attgccatgc cgatcctgat tcttctactg tattcattgt
     5041 tccggaagaa tgggagaaaa cggaaatacc ttttatggtt tttgataatg tttctggggt
     5101 gccttggcgg aatcatgaaa gccacctgct atgtcacatt gattgcggta gtactggtgg
     5161 atctggtatg gtctgcctgg gaagaggtgc caatgcgtaa gcggctgcag aagtccggct
     5221 taaaaatctt gcttctgata gcggctttcg gacttggggc tttctgtaag cagggaatct
     5281 actgttccgt acagtatgat tataatgcgg atatggagat cggctggagt aattatatgt
     5341 ataacggcct caatgaggat actaccgggg cctgttccgg agagggactt gagatcgtac
     5401 gatcttttgc tggtacggat aaaaataccc ggatacaata tgagatggaa ggaatccgca
     5461 aaagaatcga tgatcgtggt atttggggta ccatccggtt ctggctgcgt aagcaggtca
     5521 tgaattttaa cgacgggacg ttcagctggt atcaggaagg gtattttcag gcgtgggaat
     5581 atccgctgaa catagacagc tcctggaaag aacctctgag ggctttttac tggcaggacg
     5641 gcagcaatta tatctggttt acgacgatca gccagggatt atggcttttt gtactgttgg
     5701 gagtcataac agaggctggt atgcttttgt ggacggcggt gtcaacaatc cgcagaccca
     5761 aataccggac agaagagaac ctgtctgacc ggctgtgtct ttctactgtg atgatcgtta
     5821 catttatcgg tatgttttta tttgttatgt tatttgaggc aagagccaga tatctatata
     5881 ataccattcc ggtattctct gtcatggcag tttttggata ttacgaattg tatcggaaat
     5941 tattcatatt ttgtgacaaa aggcggaaat aacttcgttt tccggtagaa attgcacgtt
     6001 atgagagcct ataatgtgaa aatagcactg gtaaaatata taaacatcct gtataatgta
     6061 cagtaatgac tgagaaatga gtcaggaaca aaaaagaggg aggaagaata aatgtacttt
     6121 tgtaagaatt gcggcgagcc ttatatgaca gacgaagctg tgatgtgcgt aaagtgcggt
     6181 gtggccaagg gacaggggaa taattactgc cataactgcg gtaagccttt agctccggat
     6241 gctgcagtat gtttaaactg tggcgtagcc aataagcagg caccggcacc ggatgcaaaa
     6301 tccaaactgg tagccggtct gttggcaatt ttccttggta cgttcggcgt acataatttc
     6361 tatctgggct ataccggtaa ggcagttacc caattggtac tttccatcgt gggaatcctt
     6421 ttatgctgtg tggtagtcgg cgtattcatt gttatgggtg taggtatctg gggactggta
     6481 gaaggtatca tgatcctgac cggcaagatc gacacagacg gcaagggtaa tcccctggca
     6541 gattaatatc gggccttttc caggccagaa agaggagaaa atggacggac agaattttga
     6601 aaatgaaaac aatgttacag tagatactac tgcaacagaa gtaagctccg cagcagaaac
     6661 tgtacaggag accccggtaa cagaacagcc tgtgaacaat tatcaggatt ataccgcaaa
     6721 tgtacagcct caggcagcta caacagctcc tgtacagcag ccggaggaca aagaaaccaa
     6781 tgtactggct attgtcagcc tggtactggg tatcctttcc atcgtacttg gatgctgcac
     6841 cggatggatc ggcgcactgt ttggaatcgg tggtatcatt tgcgcagtat ttgccaataa
     6901 gcagggcaag accggcctgg caaaggctgg actgatctgt tctatcattg gtattgtact
     6961 gggaattatc attacaatct tagctgctgt attttctgta gcatttatga gtgaacttac
     7021 aaactactaa aaaccagcgg agcctggaag atcagcttta tattgcagga tggatatttc
     7081 tgctggtcgg aagcatcgga agctttctgt atcttcatat aatcgttccg agactgcagg
     7141 gatatacctg cgtggtctat cgattatttg ggatttactg tcccggctgt ggtggaacga
     7201 gagcggtaag tgcactgctt actggacatc cgctactggc cacatggtat catccactgg
     7261 ttacgtatac tgttgttatc tttggaggtt ttatgctaag tcagaccctt gccagattac
     7321 atattggcag gatcaaaggc tggcgctttc atgaatggca tctgtatgcg gcggtaggga
     7381 tcatgatagg gaactttatt ctgaagaata ttttgcttct gaagtttcac attacattat
     7441 gactgtattt gcatttttta catatacaga atattttatc gcacaaaagt gcagagggag
     7501 aaaacatgaa taaaaaaact acgggtatca ttgcttacat tacctggatc ggctggatcg
     7561 ttgctttttg cgcaggtgac aaagagggtg ctaagttcca tctgaatcag gctctggtta
     7621 ttctgatcgg ccagatcgtt gcagcagtaa tcaactacat tccttatgta ggtggtattg
     7681 ttggcactat cttaagcatc tttctgtttg tatgctggat catgggtctg atctatgctt
     7741 gccaggagca ggagaaggaa gttcccctga ttggtcagat caagatttta aaataataaa
     7801 aatgacaata aatcataaaa agaatccccg gtgttcatta catcggggat tctttttatg
     7861 ggtgcgcctg gcaacgcacg tttctgtgat tattaaaaaa ttgtaactat tctgcagcag
     7921 aagcagccgc tgtttcctct gcagtggtca gattcacatc ttcgggctgt tcaccgtgca
     7981 ggaaagtgat gatgatctgg atccgcttgt aagctctggc ataattttcc tttgcatagt
     8041 ctgcggtcag ctgattgtaa ccaccgttat cataggcatc gtggtaactc tctaccgcag
     8101 tggtcatata ttcgcttgct tcatcggcaa gagattccag ataatcaaat tcagtaggaa
     8161 aatccaacgc tgcaaaatcc tgaaaagcgc tgtccagctg atccagataa ccgagaagtt
     8221 catcggtggc attttcggat gtcgcatcta cgttgttgat ctcggtatct atatcggaga
     8281 tctcggtaca aaaagaatct atttcctcct taaactgtgt cagcgcagga tcctttccgc
     8341 aggcggtaag cgtaagtgcg gtcagcagtc cggccgtaag ggcaagtatt tttttcttca
     8401 aaattatatc ctccaaatca tttgatccat ctaaaaattt atcatattct gcagttttac
     8461 gcaaccttat aatgttaaaa cgtccccttt acgaataaaa ggacattgtg gtatgattaa
     8521 aagaatcaag atacaggaaa agagaactat gacaagagaa gaatttatta cgttaagtaa
     8581 agaccatatt ttatatctgg acggcgctac aggatccaat ctggtgaagg caggaatgcc
     8641 ttccggcgtc tgcccggagc agtggatcct ggagcatcag ggtgtcatgc tgcaattaca
     8701 gaaggattac gtgcaggcgg gaaccaatat cctgtatgcc cctaccttta ccgccaatcg
     8761 cgtcaaactg gcagaatatc atctggaaaa aaatatgact tccatgattc gtgatctggt
     8821 ggctatttcg aagaaagcgg cagagagtac tccgggacat ccggtttatg tggccggtga
     8881 tctcacaatg actggtgaac agttaaagcc catgggaaaa atgaatctgg aaacactgat
     8941 cgatatttat aaagagcaga ttctttgtct ggtggatgcc ggggcggatc ttctggtggt
     9001 ggagaccatg atgagcctgg cggagaccag agcggcactg atcgcggcga aagaagtctg
     9061 tgatttgccg gtaatcgcta cactgacctt tgaagcggat ggaagaactt tgttcggtac
     9121 ggatgcgaaa acggcggcca ttgtgttgga aagcctgggg gccagtgcta tcggtgccaa
     9181 ctgttccaca ggtccggctc agatggagag cattatttcg gagatggtat cccataccag
     9241 aattcctgta attgcaaaac ccaatgccgg acttcctttt ctggatgaaa acggaactac
     9301 ctgctacaac atggaagcag aagagtttgc agaagagatg gaagtactgg tcaatgccgg
     9361 agctacgatt cttggcggct gttgtggtac cactccggaa ttcataagac agatccatga
     9421 acgatttggt acagatgcga aggttgcagc atcccgaaga ccggatggaa ttcggtatct
     9481 gacctccgaa cgtattaccc acagctttgg tctggatgac ggctttttcg ttgtgggaga
     9541 acgtatcaat cctaccggca agaaagcact tcaggcacag cttcgtgagg gcagctttga
     9601 aaaggtgata cagtttgccg aagaacagga agcctgcggt gccaaggtac tggatatcaa
     9661 tatgggcatg agcggtatcg acgagaaagc tagcatgctt cgggctctgg aggaagtcag
     9721 cggtgtcacg aacctgcctc tttccctgga ttccagctac gtggaagtct tagaagctgc
     9781 actgcgtaat tatcccggac gtgctctggt caattctgta tctctggaga cggaaaaatt
     9841 tgaaaaacta cttcccattg tagccaaata cggtgctatg tttatcctgc ttcctttgtc
     9901 ggatgcggga ctgcccaagg atatagaaga gaagaaagag attattcaca agatttatga
     9961 ccgggcactt tctctgggaa tgtgcaaaga agatatcgtg gtggacggtc tggtggcaac
    10021 ggtaggtgcc aatccgaaag ctgcactgga aacgttggag accatccgct attgcaaaga
    10081 gaacggtttt gccacgatct gcggtctgtc caacatttcc ttcgccatgc cggagagaag
    10141 ttttgttaat accgcattcc tgacgctggc aattcaggcg ggactgacca tggctattgc
    10201 caatccttcc caggaacttc tgatgagctg tgctttggct gcggatctgc tgttgaacaa
    10261 agaagaagcc gctcttcgct atatcgagta cgccggtggc gtcagggagc gcagagaaga
    10321 gaaagaagcg gaactggcaa aaaaactggc actgctggaa aatcagggaa caaccgccaa
    10381 gacaggcact gccggcaatg ccgccaaaga aacgactgct gataacggtc cgcagatcaa
    10441 tgaaatgcag gacaagctga agacggcggt actgaaaggc aaccgtaacg gcattgtaaa
    10501 gatcacgaag gaagcattgg aaagcggaga gaaacctgcg gagctattga atcaggtact
    10561 gcttcctgcc attaatcagg taggtgaatt ctttgatcag ggcaagtatt ttctgccgca
    10621 gctgattgcc agtgcggaag ccatgaagaa ttccatcgag gtgctggaac cgttgctgca
    10681 gaccggaggc accggagaag aaatgccggt ggtggtcatt gccacggtgg aaggagatat
    10741 ccatgatatc ggcaagaatc tggtggcact gatgttaaaa aaccacggat tccatgtgat
    10801 cgatcttggc aaggatgtgc cgcaggcgaa aattctggag tcggcgaaag aacatcatgc
    10861 ggaatttatt gccctgtcag cactgatgac caccaccatg cagagaatgc gggagatcgt
    10921 ggcagccgca aaagaagaag gcatcaccgc caagatcatc atcggcggcg ctgttattac
    10981 gcaggaatat gcggatgaga tcggtgccga cggctattcc aaggatgcgg cggatgccgt
    11041 gaaactggcc aaaagcctga tgtaaaagag tctgaaacaa cagaaataat ggagtataga
    11101 taaaggagca aagcaaaatg attggagcag atgcgattat taaatgtctg gaagcagagg
    11161 gggtaagtac tgttttcgga tatcccggtg ttgccatatg tccttttttc aacagcattc
    11221 tggattctga catcaaaacg gtgttgatcc gtacagaaca gaacgcggcc catgcggcca
    11281 gcggtgttgc ccgtatgacc ggaagaccag gtgtatgtgc ggtgacctcc ggccccggtg
    11341 ccaccaatgt gatcaccggt attgccacgg catttgctga cagtatcccc gtgatctgca
    11401 ttaccggtca ggtcaacagt gaactgttgg gcagtgatgt gttccaggag gcggatatta
    11461 ccggcgccgt ggaatctttt gtaaaataca gctatctggt gaaaaatgta aatgatattc
    11521 ccagagtgtt caaggaagca ttttatattg ccaata
//
LOCUS       DAWDHD010000074        11404 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_359_length_11404_cov_14.287250, whole genome shotgun sequence.
ACCESSION   DAWDHD010000074 DAWDHD010000000
VERSION     DAWDHD010000074.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 11404)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11404)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11404
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_359_length_11404_cov_14.287250"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2787 a         2973 c         2481 g         3163 t
ORIGIN      
        1 tgcacacaag agtgtcatcc ttcgtaatga ttaaaatacg ttcagattca gtacgactgc
       61 aagtaacatg aagaatactg caaggatctt cgtgatcttc accagcatac cttccatgga
      121 acgtccttta tttctgcccc agtaggtctc agcagcgccg ctgatggaac ccagtcctgc
      181 ggatttacct tcctgcatca atactaacac tgtcagtgct acacatacca atataaaaat
      241 tatgttgatt actactctta atgcttccat ttcacacctc ctaatgtcaa gcagatttta
      301 tattaccata tattgtacac aattgcaaca acattttaca agacagtatc gaaaagacgg
      361 cacaaaaaag aacccccgga aatctttacg atctcacggg gatcctttat ttttattctg
      421 aatgatcaga tccgcagatt actctgcgat gtcaccatac atgaagtacc agtaaccata
      481 agcggaatgc cagataccgg tgatcttctc gctctgcagc cagaagtcat tgtagtaagc
      541 aacagggatg cagcctacct gctccatcag aacatcctca gcctcatgaa gagctgcgga
      601 acgctcagcg gtatccatgg tggaagctgc cttctccata gctgcatcga agtcagcgtt
      661 ggagaactta ccatcgttgt taccgttgcc ggtgcagaac agatctaaca tattggaagg
      721 atcgctgtag tctcctaccc atccgttacg tgctaccatg tactcaccgt tacgacgcat
      781 cggagtgaag ctggaccact caacgatatt tacttctacg ttaataccaa gctcgcccca
      841 agcctgctgt aagtactcag caacaacctt gtggtaacct gcatcattgg tagtgtaggt
      901 aatggtagga agtccttcac cgttagggta gccggcatca gctaagagct tcttagcctc
      961 ctccaggtta gcatcgaaat catcggagat gtagctctga ccaccgttag catttgcatt
     1021 gaaggagctg ccgtcggtat ctacccagcc ctcacctaca atgttgtatg cgggagagta
     1081 ggttccctgc atcagggtat ttgctacata gtcacgatca attgcaaggc tcagagcctt
     1141 acgaacgtta acgttctcga atgcaggatc ttccaggttc agagacagat agtaagttcc
     1201 taagatagga tctacatgga actcggggtt accctgtaag ctggggatct cctgggtagg
     1261 tacactcttg atgaacagta catcgccgga ctggaatgca gtataagctg cgttggaatc
     1321 ctccatcagt acgaacttga tgctgccaag cttgatcgca tctgcgttcc agtagtacgg
     1381 gttcttggac ataatgatgt gagaaccggg tacccactcg gtaatgtaga aaggtccgtt
     1441 ggagatatag gtctcagctg cggtagccca tgcatcgccg ttagcctcta cggttgcctg
     1501 ctgaacaggg ctcagggtag cgaatgctgc cagagactcg aagtaagggc agggattgga
     1561 aagttctaca acgaaggtct tagcatcggg agcggatact gccagagcat ccagatcacc
     1621 tgcctgtgcc tcgtcaaagc ccttaaccat acccagaaca gtctcagcat aaggagctgc
     1681 tactgcggga tcgcatacac gcttccagct gtatacgaaa tcgtctgcgg tcagatcact
     1741 gccgtcagac cactttaatc catcacgaag atggaaggtc caggtaagac catcctcaga
     1801 tacttcccag gtctcagcct gtccgggagc aatcttacca tcctggtcta cggtcagaag
     1861 acactcaaaa gagtgaagca gcatattacc gccatctact gcactgttca gtgcgggatc
     1921 aatggtctcg ggatcgggac cgatctgaac ggtaagctgt ttgccttcgg tactaacttc
     1981 agttgctgct gcatcggtct tggtgctgtc agttgcactg gcactgccgg ttgcagaaga
     2041 accattgtcg ctgctgccgc atgctgccag agaacctacc agcattgctg cggtgagcag
     2101 catagctaac ttctttttca taaagttatc ctcctctttc ttttcataca aaagcactat
     2161 gctttgtata actttatatt aaacagctaa attgctcagc tgtcaatacc taatttcagg
     2221 cattgatctt ttccacatgg tggcaagcca cgtagtgacc cttttccact tctctccatt
     2281 cgggttcctt ctgtgcacag cactcatccg cataaggaca tctggtgcgg aaacggcatc
     2341 cggtaggcgg atttaaggga ctgggtacat ctccctgcag aacaatacgc ttgcttgctc
     2401 ttgccttgat gggatctgca atggggattg cagagatcag gctcttggta taaggatgca
     2461 ggcttcgtgc taccagctcg tagctgtctg ccagctctac cattttgccc aggtacataa
     2521 cgccgatacg gtcggagata tgttttacga cagagaggtc atgtgcgatg aacagatagg
     2581 tcagtcccat ctgctcctgc agctcctcga acatattcac cacctgtgcc tggatcgata
     2641 catccagtgc ggagatcggc tcatcgcaga cgatgaactg cgggttcacc gccagtgctc
     2701 tcgcaatgcc gacacgctga cgctgtccac cggagaattc atgaggatat ctgtttgcat
     2761 gctcggagtt cagtccgacc ttctccagca tggagatgat cctgtcatga cgttccttct
     2821 tattggcagc cagcttatgg atgtcgatgg cttcccctac gatatctcct acggtcattc
     2881 tgggatccag acttgcataa ggatcctgga ataccatctg catcttctga cggtaaggca
     2941 gcatatccac ctttacttta ttttcaacat ccaggatcac attgtcgtca aatgtgatct
     3001 tgccgccggt gggctcatat aatcgtaaga tagaacgtcc ggtagtggtc ttaccacagc
     3061 cgctctctcc taccagtccg agagtctcac ctttattcac aaaaaagctg acatcatcca
     3121 cggccttcac gaacttacgg tttttaccca tgccgcctgc ggggaagtat tgcttcaaat
     3181 gttcaacctg aagtaactta tcactcattg gtatttcctc cctccatctc agctttctgg
     3241 ttcataaagc agtgtgcata atgcgtctcg ctaaaatccg tcttgggcgg attctctctc
     3301 aggcagatct tcatgcagtt cttgcagcgg ggtgcgaatg cacatccctc gggaggattc
     3361 aaaagatcca ccggattacc ctcgatgggg atcagcttct catgcttcgt gctgtccaga
     3421 cgggggatac tctccagaag gcccttggta tattcatgct tcggatggta gaagatctcg
     3481 tcacaggtac cggtctccac gatacggccg gcatacatga ctgcaatacg gtcacacatg
     3541 ctcgccacga tacccaggtc atgggtgatc atgatgatcg ccatgcccag cttgtccttc
     3601 agctccatca tcagctccag gatctgcgcc tgaatggtaa cgtccagtgc ggtagtaggc
     3661 tcatcggcga tcagaagctt cggttcacat gccagcgcaa ttgcgatcat gacacgctgt
     3721 ctcataccac cggacagctc gtggggatac tgcttcatac gtttctccgg ttcattgata
     3781 cctaccagag acaaaagctc cactgctctt gcatatgcct ttttcttatc cgcatcggta
     3841 tgcagacgga ttacttccat gatctgatta ccaatggtat atacgggatt taagctggtc
     3901 agaggatcct ggaaaataat ggaaacctct ttaccacgca ttttggaaaa atctttttcg
     3961 gtcattttct ccacttcgtg tccattgaag tgaagttctc cgccgatcag cttaccggga
     4021 taagcggtca gtcccatcag actgtatgcg gttacggatt taccggaacc actctctcct
     4081 acgatgccca gtacttcccc ttccttcagg tgaatggaga catcgttcag tgcctttact
     4141 tctccggcag gagtaaagaa agagagacgt tcatgctgta tatctaccag ataattgtta
     4201 tccatcttca ctactccttc cattaattct taagcttcgg gtcaaatgcg tcacgaagtc
     4261 cgtcaccaaa caggttaaag cttaagatga tcagactgat cagcagacag ggtgcaaaca
     4321 acaggtacgg gaatgtgatc attccgttaa tggcatcact tgcaaggctt cccagggaag
     4381 gcaggggaac tgccacaccc atacctaaga agctcagata gctctcggta aaaatagagg
     4441 aagggatctg cagtgtggtg gtaacgatca gcgtaccgat acagttggtc agcagatgct
     4501 ttttaatgat acggccatcc ttggctccca gagccttggc tgccataaca taatcatttt
     4561 ccttcagggt caggatctga cttcgtacca tacgtgccat tcctacccag tacaataatg
     4621 cgaatacgat gaagatactg atcaggttag atcctacgat ctgcatccat ccgaacccgg
     4681 gaagctgtgc cagattcgcc agcggatcct ttaacgcaaa ggacagcagc acgatcagca
     4741 ggaaatccgg tatcgtatag ataatatcca caatacgcat gatgatcaga tccacccatc
     4801 caccgaagaa tgcagatatg gaaccgatca gggaaccgat cacgaaaatg atggcagtag
     4861 cgatcagacc aaccaacagg gagatacggc ttcccatcat aacacggatg gcataatcac
     4921 ggcccatttt atcggtaccg agcacatggg ggaatacctt ctctcccgca tccatacgct
     4981 ctatctcttt ctcggaatac tgcatggggt gcagattctc gcttcctttg atctgtgtct
     5041 tgtaatcata gggataaaat gccggcacga taaaggaaaa gatcataact acaataataa
     5101 cgaagaggct gaccatggca accttatttt tacgaagtct gcggaagcca tcctgccaga
     5161 agcccacgct ctcacgcatg atgacctggc tctgcttctc ttcttcgttc gccggcataa
     5221 aatcttccgg tttatatttt acatgaaagc tcatagcatt cggttctttc atcggtatct
     5281 tccttcctgt taatcttact tcaacttgat cctggggtcg acgagcttat atacgatatc
     5341 caccagcaca ttcatgacaa ccatcagtgt tgcgaggaaa atggtggtac ccatgatcat
     5401 ggtgtagtca cggttaatga tcgagctgac aaattcattg cccagaccgg ggatcgtgaa
     5461 aatcttctct actacgaagc ttcctgtcat agtataagcc atcataggac ctacataggt
     5521 gataacgggg agcagtgcat ttctcagtgc atgcttgaac agacggaaga aaggttttac
     5581 tcccttcgcc gtagcggttc tcatataatc ctgtcccaga acatccagca tggaagaacg
     5641 catcagtctc ataatataag cagtggggta caatgccagt gcgatcacgg gcatcacata
     5701 atgcttccag gtgccgattc cgtaagtggg cagtatcttt accttacttc ccaagaaata
     5761 cagaagcaga gtacatataa cgaagcttgg taccgcaata ccacaagtgg agatcacgga
     5821 gatcacgctg tccagcgtct tgccacgatg ggtagctgcc agacatccca gtgggatacc
     5881 taccaacagc gctacgataa cggaaagtcc acctactctt gcagatacag ggaacttgga
     5941 tactataata gaagtaacgg ttcttcctct ctgcttcaga ctctctccga aatcaccgtg
     6001 caaagcatct ttcatatacg tcagatactg ctgggacaaa ggcttgtcca gtccatattt
     6061 tgcctccagg gctgcctgtg cagcagggct tactgctttc tccgagagga acggaccacc
     6121 ggggaccatg ttcatcagga aaaaggtgag tgttgctact gtaaaaatcg tgatcagcgc
     6181 cagaccgata cgttttgcaa tatatttaag catctcttca actccttgct tttcttttgc
     6241 ctacggacat tcataaaata cacttttcgt atttgtatgc aagtatacac gtacgtaaaa
     6301 tgtactttac ttttccgaca acgacttttg ttatattaca acactttttc aaggctgtca
     6361 actgaattac atatgaagca tctgccagtt tccgtgtttt tcttattcct gatttatctc
     6421 caccgtgttc gtgtgctacg cacatttgtc tgtatattta tgaaaaagtt tttcattaaa
     6481 aatagtctct gtttttcaat tcctgtatat agtgtatatt ttccagtata tgatgtatat
     6541 ttacacatta tatttttgtt cttttttctt tctcccatgt atgtctctat agttttcctc
     6601 aaaaaaaaga agaatttccg gtgcagaaaa ttcttctttt ttatacattt tttatttttt
     6661 gtagttttca ttttgttgcg cagtcactca gattttgcaa aacggctcca tatatgccgc
     6721 gacatccttt aactcctctt caatggcaag cagccggttg tacttcgcca cacgatcgct
     6781 gcggcatggt gctccggtct tgatctgtcc ggcattcagt gccaccgcta tatctgctat
     6841 ggtggtatcc tccgtctcgc cggatctgtg ggagatcaca gccttgtaac cattcttgtg
     6901 tgccatttcc accgcttcga aggcttcgga cagagttccg atctgattga cttttaccag
     6961 aatggcatta ccacaatgaa gtttgatccc ggcatccagc cgttttgtat ttgtgacaaa
     7021 cagatcatct cccactaact ggatcttatc ccccagccgt ttcgtcagct cctgccaacc
     7081 gtcccagtca ttttcatcca gtccgtcctc aatagagata atgggatagt tctctacgag
     7141 ccgttcataa tacgagatca tttctccggt atcccgcagg acttcctctc ccttcagctt
     7201 actctctccg gggaaatggt acattcccgt tttttcatta tatagttcac tgctggcggc
     7261 atccatggcg atcttgatat cctgccccgg tacatatccg gaagcctcga tcgcctccac
     7321 gaggaaatcc agcacgctct ccgcatccgg cagattgggt gcaaagccac cctcatcccc
     7381 gactccggtg gacaagcctt tctcctccag cagttttttc agattatgat aaatttccgc
     7441 acagatccgt aacccttccg caaagttttc cgcctgtacg ggcatgatca tgaactcctg
     7501 gaaatccaca gtgttcgccg catgctttcc gccgtttaag atattcatca tgggtaccgg
     7561 cagaagtctc ggagagattc ctccaaagta acggtacaga ggaagtccca gtgcctccgc
     7621 cgctgctctt gccaccgcca tggataccgc cagcatggca ttagcgccta aattactttt
     7681 attatctgtg ccatccgtct cccgcagaat ccggtcaatg tgaatctgat ccagggcatt
     7741 ttctcccagt aaaacaccga caattttctc gtctatattt ttcacggcat gctgcacgcc
     7801 cagaccgaaa tatctggtct caccgtccct gagttccact gcctcgaact gtccggtgct
     7861 ggctccggaa ggcactgccg ccataccttt ggtgcatctg ccggtctctt tgtccttcac
     7921 ggtaacaacc gctgccaccg tgggattccc tctcgaatcc aggatctccc ttccatgtac
     7981 ttcgatgatc tcctgatatt tttccataaa aaacctcctt acaggcatcc ttaacttact
     8041 ttttcttagg taagtatgtc ccataaggag gttattattc agatctgcta tgtgttaata
     8101 cgcttttccg gcaaggatct cacagtaatc cttataattt ttctcaagta gtgccggagt
     8161 atccagtcca tagggacact tggatttaca tctgccacaa tgcaggcaat cctcgatctt
     8221 acgcattttt gcctgcatct cgtctgtcag ctgtgctgct gaaggtgctc tgcggatcat
     8281 caggctcatg cgggcacaat tgttgatctc gatccctgcc ggacagggca tacaatagcc
     8341 gcagcctctg cagaactctc cctgcagttc ttctctgtca cgatcgatca ccgcctgtaa
     8401 ttcgggtgtt aatgcaggag gattatctat ataggacaga aattcatcca gctcactctc
     8461 ccgctggact ccccagatgg gaagtacatt atcaaactgt gccagatatg catatgccgc
     8521 tgcggaatta ttgatcagtc cgccggacag tgccttcatg gcaatgaatc ccatgcctgc
     8581 ctttttgcaa tcctgtacca gttccagatc cttgtcggta gccagatagc agaaagggaa
     8641 ctgcagtgtc tcatacagtc cgctttcaat ggcttcatgc gctacattca gacggtgatt
     8701 ggtaatacca atgtgacgga tcatgccttt ttctttggct tccagcatgg cctcatacaa
     8761 tcccgtgcca tctcccggct tcgggcagaa tgccgggtta tggaactggt agatatcaat
     8821 ataatctgtt tttaattttt ccagggaagt gtgcagctcc ttccagaagc cttctgctgt
     8881 ggtggcgccg gtcttggtag cgatatatat tttgtcacgc ataccctcaa aggcagcgcc
     8941 cactttctct tcactgtcag tatagtatct ggcagtgtca taaaaggtta cgcctgcagc
     9001 ataggctttg cgaagcagtc caatggctgc ttccttggag atccgctgga tgggcagcgc
     9061 tccgaaggag tttttctcta cggtgatccc ggtgctgccc agagttacca gatttttctt
     9121 ctgttccata taatataatt cttcctcctg ctgataagaa gatatcatgt aaaaatcagt
     9181 tacaaaatcc tcaggcaccg tgcaaagtgc ctgaggaatc ttcctctgct tttatggttt
     9241 ctgcgatctt accgaaaata ttccggtgat tactttttaa ttaacagaga ctttcctgtc
     9301 atttctgcag gctgctccat acccatcacg ttgatcaggg taggtacgat atctgccagg
     9361 catccgccct ctttcagcgt ataagcagga tcgtagttta ccaggatgaa cggaaccgga
     9421 ttggtggtat gtgcggtatg aggctgaccg gtggtgtaat cgatcatctg ctctgcgtta
     9481 ccgtggtctg cacagatgaa cagaacaccg tctacatcct tcactgcctg tactgccttg
     9541 cctacgcact catctacggt ctcaatcgcc ttgatggctg cggagatcac accggtatga
     9601 cctaccatat cggggttcgc gaagttgatc acgatcacat cgtatttttc agagcggatc
     9661 gcatcaagca gtttcccgca aacctgagga gcactcatct cgggcttcag atcgtaggtt
     9721 gctacatcct tgggagaatt tacgagaata cgatcctctc cctcgttggg ctgctctacg
     9781 ccgccgttga agaagaaggt tacatgtgca tacttctcag tctcggcgat acgtgcctgc
     9841 ttcatgtcat gggctgccag ccactcaccg aaggtattgg taatggcgat cttgtggaat
     9901 gccacttctt tattaggaat ggtaggatcg taatcggaga agcatacaaa agtaatatcc
     9961 tttctcacac ggtcaaatcc cttgaagtca tcatcacaga aggctcttgt gatctctctt
    10021 gcacggtcgg gacggaagtt gaagaaaatt acggaatcct tgtcctgcac cagtccgact
    10081 gccttgccgt cttctacaac aacagtaggt tttacaaact catcggtctc ttctctgtcg
    10141 taagactcct ggataccgca gataccgcag gcagcggtca gacctttgcc ttcggtcatg
    10201 gcatcgtaag ccagctgtac acggtcatag ttgttatcac gatccatagc atagtatcgt
    10261 cccattacag aagcgatctt acctacaccg attttcttca tttcttcatt taactgctct
    10321 gcatagctct taccgctggc gggaggagta tcacgtccat ctaagaagca gtgaacatat
    10381 actttttcca ggccctgctg cttcgccagc tccagcaggc cgtacagatg ggtaatgtgg
    10441 ctgtgtacgc caccgtcgga taacagaccc atcagatgca gggcgctgtt gttcttctta
    10501 cagttatcaa tagcctttaa cagagcttcg tttttgaaga aatctccgtc ctgaatctcc
    10561 ttggtgatac gggtcagctc ctgatataca atacggccgg cacccatatt catatgacct
    10621 acctcagagt tacccatctg accttcggga agacctactg ccataccgct ggcattacca
    10681 tgtacaaaag gatactcctt catcagggag tccattacag gggtattggc ctgtgctacg
    10741 gcgttgccct ctaccttatc attcaatcca taaccatcca ggatcattaa tacaaccggt
    10801 ttctttctca tcgatcgttc ctccattttt acttttgatt ataataaatt accagtacgg
    10861 ctccggaaaa tggtctgcgt cccggatccg ttctgaaagc ttacattacg tccaccgaga
    10921 ttatacactg aaaacggcgg atgcgcaata gtaaccgctg atttcttgat ctgcatcttt
    10981 gggcgttttt tctggcgttt tttctgcaaa tcatttttct gcaaatcatc cggatttact
    11041 tgtatttttc ctgcatatgc gctacaatat cccttaaact ctcatttggg aaaaggggat
    11101 atcttatggt tacattattg gcaaaattat ttatcaggga tcatgaaaac gttacggatt
    11161 ccggcgttcg ccaggcatat ggaatgctct gcggtatcgt cgggattttt ttcaacctga
    11221 tcctgttcac taccaaggct ctggcaggct ttttcagtca ttccatcgcc atcacggcgg
    11281 atgcattcaa caacctgtcg gatgcagcat cctccatcat tacgctggca ggtttcaaaa
    11341 tggcgggaca gaagccggac tctgaccatc ccttcggaca cggacggatc gaatatatct
    11401 ccgg
//
LOCUS       DAWDHD010000075        11316 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_364_length_11316_cov_14.538762, whole genome shotgun sequence.
ACCESSION   DAWDHD010000075 DAWDHD010000000
VERSION     DAWDHD010000075.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 11316)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11316)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11316
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_364_length_11316_cov_14.538762"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2579 a         3055 c         2455 g         3227 t
ORIGIN      
        1 ctgttttata aaatataaaa accttagtaa ctggaagtta ctttttcaat tgagtagaac
       61 aggcaaatgc ctgtcctgtt tctgttttcc gtccttgcta taatgcaatc ttgaataccg
      121 ctttcttcac tttaccgaca ccgcccgacc ggacacaggg cttgtgtgcc tctcccggaa
      181 ggagtatgac cagttctcct gcgtgcagat ccaacagaga tgctttgcca tccttccaga
      241 accggatatc tgccttagga tcgtacttgg tgtccggtgt gggaagtggc tgctctgtta
      301 cccagatctg ttcctttccc tgcagaatac actggatatc cgcatatctt ttatgtgttt
      361 caaagcggca gtccatgtag gactttgtct cgtactcctg gatgctgaca tagatctgtt
      421 ccccgtcgat ctcatatttt ccgcagggct gcgccgccag atcccgggag tttacatatt
      481 cgcttacctt ctctatgcac tctgccggta ttccgacctg ggaatactgc ggcagagcgt
      541 ccatatatcc tttgaccatt gtgtatcctt ccggcggtat tatttgtaca tgggaccgta
      601 tgcggcacag gcacgataat cctgtccctc tttgtccatt ccgaaggcat tccaggatgc
      661 cgggcggaaa attttctcct ccggtacatt gtgcatgctg accggaatac gcagcatgga
      721 acacaaggta atcagatcgg cacccacatg cccatagctg atggctccat ggttggctcc
      781 ccagttgttc ataacatcat aggcactctt gaagggtccc tcttttccgt tgcatctggg
      841 tgcaaaccag gtgcagggcc aggtgtaatc ggtacgcttc cataagatat ctgtcacttc
      901 ttccggcagc ttcaccgtcc agccttctgc gatctgtaag gtggggccga tgcctttgac
      961 cagattcaga cggatcatgg tcacaggcat ctgtgcctct gtcacgaagc gggaggaata
     1021 accgcctccg cggaaatagc cgaagtctgc ctcactccaa gtcgttgcct ccagcattgc
     1081 cttctgatct tcttccgtca cctcgtacca gggcttcatg acagagttac cctctgcatc
     1141 cctggcttgt ccgctggcat ccagacatgc cgcaccggaa ttgatcagat ggataaagcc
     1201 gcctgcttcc ttcgccactc cttcgacttc gtagccggct gccttcttca cagcctccgg
     1261 gctccagtaa gtacggacat ccgcaaagat ctgtgcacgg ttggtcagaa gcttcaaaaa
     1321 cagcattccc aatccgttta atacatcgtt ttccgttgcg agaatataag actctcttgc
     1381 gccgttccag tcaaaagtag tattcagcat tgcctcggca aaatccgtat tgggatagta
     1441 atccgtccac tgtctctgtc cctggaagcc tgcagcaatg gcattgtgtc ccaccatttc
     1501 ctcttctctt ccggcgggta atctgtcatt accgttcatc agatccttga tgatgcacat
     1561 ggtctttaca acgaatttcc agtcctcttc cttctcttcg ggagacttcc ggatctccac
     1621 aggattcttg tccagtccgt ccggacaatg ctccctcgtc cacttataag ccttctcaaa
     1681 ttcatccttg tcataaatat cctgctggat acggcgcaga atctccactt catccacgga
     1741 ttccacacgc attcccagat attcctcaat gaatgcagga tcaataatgg atccaccgat
     1801 acccatacag atagaaccga tctgcagata ggatttgcct ctcatggtag ctacggccac
     1861 ggctgcccgg ccgaagcgca gcagcttctc ctgcacatct tccggaatct ctgtagcatc
     1921 cgcttcctgc acatcatgtc catagatgcc gaacgcaggc agtcccttct gggcatgggt
     1981 tgccaatacg gatgcaaggt atacggcacc gggtctctcc gtaccgttaa atccccagac
     2041 acccttaatc gtattgggag acatatccat ggtctccgct ccgtaacacc agcagggggt
     2101 cacggtcaga gtaatggcaa cgccttccga ttcaaaaaag tccgcacatt ctgccgcttc
     2161 tgccacgcgt ccgatggtgg taggtgagat cactaccttt accggctctc cgttgctgta
     2221 acgaagattt tcttcgaaca gctttttggc agaagaagcc atattcatgg tctgttcttc
     2281 cagtgagcct cttacattca gataaccacg tctcgcatcg atcacgggac ggattccgat
     2341 caccgggtat tttcctatgt atctgttttc agccatattg tcctcttttc tgcgctgcct
     2401 ctttcttttt catttcgttg ttctgcctct gccatgggac tgcggtagca gcgccggtgc
     2461 agtcctgaga tctcctagcg ggcaattccc agttcaatct ctacctctgc ggacagacga
     2521 atgagctgct ctgcttccat ccgctgtgcg accgtcagtt tcttccagtc taatcttctg
     2581 ctgtgcagcg tcatgggaac tcccatcttc tgtaatgcat atttggcgtt cacgggatag
     2641 ctgctgctct cgatcaggga gcacatggtc agcagatctg tcactttccg ggctttctca
     2701 ggctgtgcgg catagttttt gcataaccaa ctgtacagtc tcggatggaa attcgccatg
     2761 actccactgt agccggcggc tccctcctgc aatgattcca gtaaggtcgc agtattggca
     2821 ttgtaaagct tcagattgct tccctggatc agctgcaatt tttcgctcat gcttgccata
     2881 tcacagctgg tatccttcag aaaatagaat ctctcactgt ctaccagaaa cttcaccact
     2941 tctctgctca gtactctctt gtagggataa ggacattcat aaaatccaag cggcagatcc
     3001 ttcggaagct gttccatcaa tgtctgcagc ctttcgatca tcacctgatc ggattcctgc
     3061 tgtgcagcag gcctgttaga gatcaggatc agcgcgtcga ttccggtatc tgccatgcgt
     3121 ttcagttctt ccgcctgatc ctccatggaa tcagaaatat gcccggatgc aatcaccggt
     3181 ttcccgttgg catacttctt tactgctgct gcgattttac agcgttcttc cagtgtcagc
     3241 tggaacattt cactggattg gcataccgca aacagtccgt cacagtcatt ttcaaaatac
     3301 cattcggtca gtttctccaa cgccggatag tcaattttat tatcctccgt ataaggagtc
     3361 agcatcaccg gccatactcc attaggaaat tcgtttttca ttattgctcc catctgcccg
     3421 ccctgcggac ttttttattt gtatcttacc tttccatcag taccggtgcc ttaaggattc
     3481 cgtagggttc caccttatat tccggtgtat atttttcacc gctggtacga aatacagaat
     3541 cgttcgtcac ttccggtctg cctcccgcga gatgaaaagg accgaataca ttccgcaaag
     3601 aactgtaaat ttctatctca aggtgattgt caccttcttc taatgcctct gtaatgtcga
     3661 tcattcccgc tgctctccag ggaacttcat aggaagccat tcccagtctg accgtagcgc
     3721 aagtaccctt gaaatctttc aatttcaaat agatccggct gtccgctctc cttttacagc
     3781 gtggattcca tcgaaaatca tacagatatt tgacgctgcc tgcataatgg tacaatccct
     3841 gagccgtcca gtctccgatc agcagtgctg tcggctgttc tgtaatcttc cgttccgtat
     3901 ttacgccgaa ctctccgcag agataacagt tttccagttc catggaatta cgataacggc
     3961 agatcagccg cagcctgttc tctccttctg cccagcccgg aagcggaatc ctcttgatcc
     4021 ctctgtcata caggcatccc gtgatccgct gttccaccgg ctgtccgttc agatagatct
     4081 gaaaattttc cggatgttcg attgcaagtt ccgtgtagcc tgtaattttt ttcaccgtct
     4141 caaatgtaaa ttccagtgag acttccgttc cgtccgcagg atgaggttct cttacccaac
     4201 ggtaacgctg ttcttttcca agggctgcta tggagcacat ttccaatccg gtgcggatct
     4261 gttcctgtgc ttcccagacc tgcataggtt ctgaccagtg cccctcctgt atgcggtatc
     4321 tgcagctatc cagagtatac aggttgggat cggtcagacg gatcactgtc cgttccggca
     4381 gcacctgaag ttctgtttcg tcagcctctg caggcactac gcttgcgcca gctgattttt
     4441 cggcgttttt ctccattttc tccaggaaaa acaggcgcga agtctgtgct tccaaccgtg
     4501 tcggaaaaac agtctgcccc tgtccggtga cagcttccac cgtattatga gttccggtga
     4561 gtgcgtccca ctcttctaca gaagcttcca agggtacgtg aatctctacc tcacagtctt
     4621 ccgtccggtg atagtttgtc acgaacagaa gctgtccctt ttccagaaat ttctgcaagg
     4681 cgatacagtg ggtttcttcc ctgtctccgt tccggatgct tactgtccgc actcctgcgt
     4741 cttccagtgc ttttataagt tcttcatcac tgtgtaccgc cacacaattt ttagcggtaa
     4801 acagttctgc cggaaggtcg ctttccatgg catccagcag gaccgattcc ggatcggtca
     4861 cgatcaactg accgccctgt tccacaaatt ctttcagaag ccggcaggta ctgcccagca
     4921 tagtacggat tttcggcaga accactgtcc ggtactgcat tttccccagg atcagtctgc
     4981 cctgttctac agatccgtgc ctagccaaca gaatctcatc ccccagatcg ggatccagat
     5041 gatgattgca aagcttttcc agcagcgtat tatattccca tccgtattcg ttgaccttct
     5101 gcagatcccg ttccttgcgg cgcacaggat tgccataggg actggccccc atcatgctcc
     5161 atgccgtagt cgccggatgc agcaacagat actgctggct gggagaacct tcttccagca
     5221 cggcactcag ccgcccgaaa taatcctcca ccacatgatc tttactccac caggggatat
     5281 tgtagccgaa gcccggagga cagtctctct tcctgccgcc cgtcagggaa tataacgcca
     5341 gatgctggca tcgcttattc acgcccaatg cgtattgcca gtctccgata tgccgctgtc
     5401 cttcgaaggt aaaatcccat ccggtcacgc cataggtctc ggagatgaca ttctttttcc
     5461 ccatctggtg tgccacgctg acacattgct tcacggtaag aaattcgtcc gtcttatcct
     5521 gaaggatatc cactcccgga atatcctcat attcatacag tggcatggtc gctccgctga
     5581 ctctggtaga tagcccaagt ttatcttcct gaaggaaatg acctgtcata atcaaatgat
     5641 gctctctgca ccatacagca atctgtttgg aatagttttc actaaaacgt aatgtaatcg
     5701 catgccaata atcatgccgt attttcgcac tgccttctcc ttcaaaaaag aataaaggaa
     5761 ttttgtcaac aggatcatag ccgttcttct cccggaaata ctctgcgaat ccgtaagtcc
     5821 agggaatcca gctccggtct gccccaaagg cggaatgtgt gtccgccaga ctgggttcat
     5881 ctgtaaaaaa tccgggaatt gttttgccga attcctctcc aaaccgttcc agatatttat
     5941 catgggtcag atgcagaaat ctctgtaccg tatccggatt cagattgtcc accggtgttt
     6001 ccccgttgaa ccattcactt tttgccgata tttccaggcg cagaacgaga aacacctgtc
     6061 tgcatccggg atgctgtttt ttctcttccg gctccggaac ttctgtcatg gcattttcca
     6121 gccgcttcag aaaaaaaatg gcatctccct gtaccaatgc ggtaaatacg gcaataagat
     6181 gttcttcctt ccataccgca gagtcacagg tgtcgcatac ctgcagggtc agtcctttca
     6241 gacgataggc atctcctccg gctgccacca ttcctccggc attgccggag ggccagcggt
     6301 catcatcgta cagccaggcc gttatgccca gttgttttgc ttcatccacc gccgcctcca
     6361 cgcagtccat ccattctctg cccagatagg gagtctccag accatcccgg gagtgaataa
     6421 agaatcctcc gactccctgc ccataaagct ctcttacctg acgtctgact tcttccgggg
     6481 tcatttccgc attccaggac cagaaaggag ttccccggta ttcttttgcc ggattctgaa
     6541 attttctgta gatgtcttcc tttgtcctgt tgctgtcttt cataatcagt cctctaatgt
     6601 aaagctcaca tacacaatct tttcccgttt ccaggtataa gtgatcataa tcttattgtt
     6661 gtcgttgcag attacggaag gataacaata atcgcctctc tgatcctcta acgtacagag
     6721 gatctcaaag gtctctccgt tgtctctgga caatgcaacg ctaagcggtg ttctcggtcc
     6781 cttgtaatag ccgggcagat tctctctggg attgtagacc agcaccagat cgccgttctt
     6841 taatttgacc agatcgatac cgctgttatt gttgggaagc ccggtatcat atgccagaca
     6901 ccatgttctt ccgccgtcct cggaatcgct gcggatgata cgggagctgc tgctgcggca
     6961 gagcatatgt acttttccgt cccgatcctc ccagagggta ggctgaataa tcccttttcc
     7021 gtagatatag tgtctgtcat aaggtctgtc gatcacctgc acattatgaa cgcccttatc
     7081 tgtgatcgcc agtctgtgca gcggcaccag atcgcttctc tcccaggtgc ggcagtcatc
     7141 tttggagata tccacgaatc cgtcccacaa ttccccttcc agagatccgg gagccagtac
     7201 ggtgccgtca cttaaccgga taggtttatt cttgacaggg cctcttcctc cgatatctcc
     7261 gggtaccagt tcctgcggct ctgaaaaggt gtgtccttca tcttcgcttt ccatatacca
     7321 ggtcacccat tccgagatca gcttgccgac cttatagaaa aggataattt ttccatcctc
     7381 cttacggaaa agtacgggat tccacatggc aactcctctt acatccgctg cctttaccgg
     7441 ttgtccccag ccgtccttat cacgtattgc tgtccagatc gctacgtccg ggttcttttc
     7501 ccatgcaccg ccaaaccatg ccgccagaat gccgccgtcc tttaattcca gcagtgttga
     7561 ggcatgggca ctgtcaaagg gtttgttatc atccgtgata aattcctgtg tataatttgt
     7621 aatcttcata ctgtcagtca caccttccat tgcctgattc tctcttttta gcagaatgaa
     7681 cgggtaacct gcccgttcac tctgtgttat gttctgttat gttctgttac tttcctttgt
     7741 ccgatgacct atttggtttc tgctacggca cgggcttctg cgtcattcca cagctgctcc
     7801 agttctctta cgcccatatc ctcagctttc ttgatcagtg catcccagtt gtcgatggat
     7861 tccttgccgg taatgtactt atcgtattcc tgtgtgaaga aggtatccag atcagtggtc
     7921 agttctttca cacggtctgc ctcttcggta gtaaaggaag gtgcaatgtg aggctgtaca
     7981 taagcgtcat cctcattgat gatcttccag taatcttctg tcagatcgga ccagcttccg
     8041 gtgatcatct ggatctcacg ctgggtctta atgttgcatg catacatgga gaagtccagc
     8101 ttgcctgcac caatatctgt ccagcatgca tagtagtcag aaggagatgc atccttgaac
     8161 tgcattacat aatcggaaat atattctgcc tcgccgtctg cgttgtactg gaaggtctca
     8221 ccctcgatac cgtagttggt aatgtcggaa gcctccatgg agtacatcca atcgatgaat
     8281 ttaatgatct gatctttatt cttggcattt gcattgattg catacagctt accgaatctt
     8341 gtatcgtagg acacggcacg tctctcaccc agactgttct cgggaatcgg cagtacctga
     8401 aaggtagcat tctcgttacc gtcgatctta cgcagttcgt tggtgtagtt ctgaccgaat
     8461 ccactgttat ctacgaagaa gaaggactga ccgctgctgc acttggttgt catggaatca
     8521 gaatcggttg ttgcatattc ctgatccaga acgccggtgg tatatgcctt gttcaggaac
     8581 ttcagaactt ctttgaaatt ggtatctgcg ggaccgtata catacttgcc cagatcctca
     8641 tcccagtaca taccggagct tgcggataca gaaccgaagc cggaacctaa catgtaggaa
     8701 gtggtcttta acaggtttgc agttcccttt ctgattgccc agggagtact gtcaggataa
     8761 atctctttta actgtgccag tgcatccagt acttcatccc atgtggaagg agttgcgatg
     8821 ccgttctctt ccaaaaggtc ggtacggatt acggggccga agccgttggc ccactcatca
     8881 cgaagtacta cagggaatac atacatttcg ccgtctacca tgtatctctg catttcggga
     8941 tactgctgcc agaacttgta gaagttcggc atggtctctt cgttgatata ctggctcaga
     9001 ggctcaaaaa taccctcgga tgcgaaatcg gaaatatcac ttgcgcttct cagataaatg
     9061 atatccggga aattctgtgt agacagcgca atgtttttct tctcatcata gctgctagaa
     9121 ggtacgatct ggaaatccag ctttacattg gttgcttcaa aaattgcctc gtgggacttg
     9181 ctgtcctgtg ccatgggctg ctggctagag tccatgacca taatgctgat ctccagagga
     9241 tcctcgctga tccaagaagg atgctcgctg tttgcttcct gtgtccccgc acttgtagcg
     9301 ctctcctgtg cattgtttgt tgcggcatca ttgctgccgg aagcatcgga accacatcct
     9361 gtcaacagcg atacgctcat tgctgttact gccaataagg ctgtcagttt ttggaatgct
     9421 ttctttttca tttaacctct ccttttctta aatacattct tactttatag tttgtttctt
     9481 tatcccttaa ctgctccgac cataacaccc tttgtgaagt acttctgtaa cagagggtat
     9541 acaagcagaa taggtacgat ggaaataatg atcaccgcat atttatagtt ggttgcgatg
     9601 atgttgacac ttcccgtgat ctcggaggtg tcaccctgta ttacgatgtc acgcatgaca
     9661 acctgtacag gatatttttt ctgatcattc aggtacagca tggaagggaa gtagctgttc
     9721 caatggtaca cggcatagta taaagtcatg gttgcgatga tcggtttgga cagcggcagg
     9781 acgatcttac tgaagatcgt aatgtcattt gcaccgtcca gatatgcgga ttctaccagg
     9841 gaatccggga tcgcctggaa ggaagtcctc atgacgatca tattataggt actgatggcg
     9901 ggcggcagaa cgatggccca catagtgttg atcatatgaa gcttattgat caccagatag
     9961 gtagggatca taccgccgtt tacgaacatt gtgatcatga tcagcacggt aaatacctta
    10021 cgtccgtaaa aatcctttct ggaaagagga tatgcacaca ttgcggtgag caccagacag
    10081 atcagggttc ccagacctgt gtataaaaaa gtgttcttat atccgctcca gattctctgg
    10141 ttctcgaata ccgccttgta ggcatcgaaa ttaattcctc tggggaacag tgttaccagt
    10201 ccctggttga tatactgcga agaactgata gacacaataa atacataaaa catcgggtag
    10261 aacataatga atccgaggat cgccagcaga aggtaattca caacatcaaa tattttgctg
    10321 ccgattccct gcggtttttt gctttttatt tttactttct tagccatttc tgtcctccta
    10381 ccacaagctg ctgtcactga gttttttcga tatctggttg gatgctacca acaggatcac
    10441 accgattact gactggaaca gacccaccgc tgtagaataa ctgaagtctg cactcagaat
    10501 acctcgtctg tatacataag tggaaataac atccgcagtc tcataagtag atccgttata
    10561 caacaggatt attttctcat atcccaggga catcaatcgt ccgatctgca tgatcagcat
    10621 aatggagatg gtcggtgcaa tagagggaag cgttaccgac ttaattctct ggaaacggtt
    10681 tgctccgtca atcattgccg cctccagaat ctcctggtcc acagaggtca gtgccgacag
    10741 ataaataatg gaagtccatc cacaggtctg ccagataccg gatgccgtaa atatggtacg
    10801 gaaccattcg ggcaatacca tgaactggat cttatcaaaa ccgaatgccg cgataagctg
    10861 attgatgatt ccgtccagag acaggaagtt cattaccata ccacagacaa ccacagtgga
    10921 aatgaaatgg ggcagatagc tgacggtctg gatcactctc ttgaacttgg catttgttac
    10981 ttcgttcaac atcagtgcca ggagaatcgg taccgggaag ccccacagca gtccatagat
    11041 atttaacagt aatgtatttt tcaccagttt ccagaaataa ggatccatca ggaacttctg
    11101 gaaccattta aagcccaccc aggagcttcc ccatacaccc ttcgcaatct tgtaatcctt
    11161 gaatgcgatc agtaccccgt acaaaggcag ataatggaac aggatgtaat agatgatcgg
    11221 aagtaacatc ataatgtaca gatatttgca tttcttgatc ttcttccacg gcgatgtttt
    11281 catgttatct ctccttctgt gcaatcattg tttctt
//
LOCUS       DAWDHD010000076        11004 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_369_length_11004_cov_13.110330, whole genome shotgun sequence.
ACCESSION   DAWDHD010000076 DAWDHD010000000
VERSION     DAWDHD010000076.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 11004)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11004)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11004
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_369_length_11004_cov_13.110330"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3020 a         2294 c         2887 g         2803 t
ORIGIN      
        1 ctctctaaca gatggcaggc atgggtcagt ctcagatgca tcaggtattg cgtcagagtc
       61 agatgaaaat gctgcttaaa atagcgggac aaatatttct cggataaatg aaattgctct
      121 gccagggttt ccaaccgaag ggggtccgtg tagttatact gcagataagt cagtatctca
      181 cgctgcatcg gatcgttcct tttggagccc atcgggttaa tggttccaca ggaagcaatt
      241 ttttgtataa gtgacaacaa taaaatacgt aatcggaaat acccactcaa tgtatttgga
      301 agctgtctga cagacaggat ctcacggatg agagaaatga tgtcaggaca gcttttttcg
      361 tgggaaagat tatgatgcaa aagcaattca tggttacgca ggggaaggag aaactccgtt
      421 tccaacagat cgtccgtctg aaaggaaata aattccagtg gaaacaacag gatatgatag
      481 tctacgcccg gaattgcgga acccataaaa tgaagctcac cgggattcac aaagaatacg
      541 gagccgccgg ccgcttcgta ttcttcccct tcaatgatga cattaagaga gccggagcgg
      601 acatatatga tctctacctc cggatgccag tggacaggaa tctgaaaaaa agcaggcata
      661 tcatgcaggt ggtaacagct aaaaggaaat tcttttgttc cgtgaatgcg ttcttctctt
      721 aattcagggt acataggcag gcctttctgt gaagtggagt agaggatatt atggtcagat
      781 tactataaaa gtagaaataa gtcaattttc tgtggtaaaa atgcaagttt taggaaagat
      841 aaagtagtaa tatgtaaaaa ttgaagagac tattcggaat agtattctga atagttgtaa
      901 aatgaaaatg caaattttta ggaaccgcag aaaggatata aaacctacga tggaaggaca
      961 gaataagtca ttatttcaat tatttgtacc actgtgtctg gagacgatct tttacatgtt
     1021 gtccggaatg gtggatacct taatgctttc ttctgtggga gatcaggcag tcggagcagt
     1081 cggaacagca aatacttata tcggtgtatt tattattatg tattcggtag tttctatggg
     1141 aatgctggct gtgatgacac agaatatcgg tgcgggcaga ccggggatcg cctgtcaggc
     1201 aaagactttg ggattatggt ttaatgggat cctgggtgtt gtgatgtctg tgtttttgtt
     1261 tttctgttcc gatgggattc tgtatgtggt aggtattgca ccggcattga aggagcctgc
     1321 tgtacaatac ctgcggatcg taggaggcgg aagtatacta aacgcaatga ttcccatact
     1381 ggcaggatat ctgcgtgcat ttggatatac gaaacagcct ctgatcgcaa ctgtgacagg
     1441 taatattttg aactttatat taaatgccgt gtttctgttt cgtttccacc tcggtgtgca
     1501 gggggtcgca tccgcaacag tgatctccaa agtagtgaat ctgatattga tcgtgatctt
     1561 tgtacggcgg ctgatccctg caaaaaaata ttcggagagg gtaaattccg gagagatttt
     1621 gcggaagatc atccggatcg gattaccttc cgctttggag aatactattt acaatatagc
     1681 gatgacactg gtgatccgtt ttctgaacca gatggatacg gagggaatca atgtggctgc
     1741 ccgctcctat acggcacaga tcacgaattt atccttctgt gtgggaagtg cacttgccca
     1801 ggcgaatgct attatgaccg gatggtacat gggtgcggga aaatatgaaa tatgtgataa
     1861 gcagactaaa aaatctgcgg cgatcggagc ggtactggcg atgggaacag aatttctgtt
     1921 tgcagtcagt gccccggtcc tgatctcttt tttcacgcag gataagagta tggtagaact
     1981 agtgcagaag ctgatgttta ttgatattgc cctggaagcc ggacgagtaa caaatctggt
     2041 atacggaaat gcactgaaaa ccagtggaga tgctgtattt ccggtgatcc tcggagctat
     2101 tttcatgttt ttgtgtgcag cgggaggaac gtatctgttg gggatacagc tgcatctggc
     2161 agtagtgggt gcttatatcg gtctgacagc agatgaatgc tgccgtgcgg tcggcatgat
     2221 gatgcgctgg cggagcggag catggaagaa aaaaggattg atcgaagatt gatcgaattg
     2281 caataataag acggtatacc ggctggcttt tgcccgggtg ggcattctct tgcttttcgt
     2341 agcttatcat agcatagcgc attttttata attaaaatat tgacaatgtg aaaaaatgtg
     2401 ttatagttct aaatgcaaat gcaactcatt tgcatttaga acaaatatat gattaagtat
     2461 tcatttgata aagaaagtat ggaaaggtaa gagtcatgtc ggaagttatt ttagatactt
     2521 taatagatag tataaagctg gtgccatttt tgttcctgac ctatctggct atggaatatc
     2581 tggagcataa ggctggcgag aagaccacgc atatggtgcg taaggcaggt aaaatgggac
     2641 cgttgatcgg tggcgtggca ggtgtgctgc ctcagtgtgg attctcagca gcggcatcca
     2701 acttatatgc aggcagggtg atcaccctgg gaacactgat agccatttat ctgtccactt
     2761 ctgatgagat gctgccgatc ctgatttcgg agaaaatgga tatccgcttt gtactgggtg
     2821 tgctgggagc gaaagcagcc attggtgccg tagccggatt tgtgatcgat ctgttgatcc
     2881 gggagaggaa gatacatcct catgaccacg ttcacgggca tgaggaaaat gatcatgaag
     2941 aggaagagca cattcatgaa atctgtgagc atgagaactg ccattgtgaa aaggatggca
     3001 tttttctatc tgcggtaaag catacgcttc acattacctt tttcattata gtcattggat
     3061 ttgttctgaa cacagcactg tattttgtgg gagaagatgt tctggccgga ctgatcctga
     3121 accgtcccgt actgggacct gtgctggcag gagtggtggg actgattcca aactgtgcag
     3181 catctgtgac cataacgcaa ttgtatatca gtggtgtgat cagccttgga gccatgatgt
     3241 ccggactgct ggtaggagcg ggagtaggac tgctggtgtt gttccgcgtg aatccggata
     3301 aaaaggaaaa tctgaaaatc gtgggaattt tatatgtgat tggcgtttta gcaggaattg
     3361 tgataaactg gctgtaggca caagcagtgc caaaattgag agctgactct ttgactgctt
     3421 gcaggcataa gaggcagatc caattttgat agggcaacgc ccgtccagtg agggaacgca
     3481 gggcgttacc gaactgagca gcactgcgga tgccccggat cgcgatatta aaggacagat
     3541 atctatggaa gaacataaat gtgaacacaa tgatatttcc taccctaagg gattgaaatg
     3601 gaccaaacag agaaaatgcg tgtatcaggt actggaggaa gcgaccgaac ccctcagcgc
     3661 cgtgcagatt tacaatcgta ttgagaaaga acacgaaggc agcgattatg ctgtatccac
     3721 aatctaccgg atcctggcaa catttgaaga acacggaatg gtagagaaga ctacgttcat
     3781 gggagacggt accgtagtat acgaattgaa ccgtggggaa catacccatt atgcggtctg
     3841 cctggaatgt cataagcgga ttccgctgcc cagctgcccc tttgcacacg tacatctgga
     3901 acaggatacc ggagatttta ccgttaccgg acacaaatta gagctttatg gctattgcaa
     3961 aaagtgtcag gaagcccgcg aaaatcacta atttcaccta ttatataaaa aaatcgaaaa
     4021 aaatatttaa ttttttttga aaaagtactt gcattattta aaatcatgtg ataacatatg
     4081 tcttgtcgtt gcgggagaca caacaacgac aaagaaaatg caccgctagc tcagttggta
     4141 gagcacctga ctcttaatca gggtgtccag ggttcgagcc cctggcggtg cactcgagag
     4201 aatcgttttt ggagacatag gagctccggg gacggttctt tttttgcata gataccctga
     4261 ttaaggagat ttcacatgac tgcgatattt gatacccatg cacattatga cgatgacgca
     4321 tttaatgaag acagagaaca gcttctggcg gcgctgccgg agcaggggat tgcccgggtg
     4381 gtggatgtag gtgcgagcct tgcttcctgc aggaaagtac tggagctgat ggaacgatat
     4441 gattatatct acggagctat cggcgtgcat cccagcgaga cagcggaact gaacgaggaa
     4501 acctattcct ggttaaaaga gcagtgccag aaggagaaat gtctggcggt gggagagatc
     4561 ggactggatt attattggcc ggagccggat catgagacac agaagaaatg gttcctgcgg
     4621 cagctggatc tggcgagaga gatcaaaaaa cctgtcatca ttcactcccg agaggcggca
     4681 aaggataccg tagacctcat gacggaggca catgcagagg agatcggcgg cgtgatccat
     4741 tgctattcct atacgaagga aacggcgaag actttcctgg atatgggctt ctacttcggc
     4801 attggtggtg tgctgacttt caaaaatgcg aagaagctga aggaagcggt agagtacatt
     4861 cccatggatc gcattgtatt ggagacggat tgcccgtatc tggcaccgga accgaaccgc
     4921 ggcaaacgca acagttccct gaatattccc tatgtgatcg ctgccatggc acagatcaag
     4981 gagatcacag aggaagaagt gcgtaaggcg gcatgggaca attctctgaa attatatcgg
     5041 atggataaat aatcgggtaa aaagcaatgg aagcaataaa agaaatggaa gaaagcaaga
     5101 caaaacagca actattttca aaagaagatc tgaaaaaact aatcgttccg ctcttatttg
     5161 aacaggcgct ggctattacc gtgggtatgg cggatacggt catgatctct tcggcgggcg
     5221 aagcggcggt ttctggcgta tccctgatcg atatgttcaa taacctgatc atcagtgtgt
     5281 tggcggcatt ggctaccggt ggcgcggtgg tgacttccca gttcctcgga gcagggaaaa
     5341 aagaggacgc ctgcaaatcc gcaaaacagc tgatcgtgtc ctccgtatgc attaccattg
     5401 gtatttcgat tctggtcatg gtgttctgta acggcatcat ttccctgttt ttcggtaaga
     5461 ttgaagcgga tgtattccac aatgctgtaa tttacatgat gatctccgca ttgtccttcc
     5521 cgtttttggc tttgtacaat tcctgtgcag cgctgttccg ctccatggga aattccaaga
     5581 tcacattgaa ggtctccgtg gtggaaaata tcatcaacat cgggggaaac gcccttggtg
     5641 tctatgtgct gggactgggc gtggccggtg tggcgatccc ttccctgatc tcccgtggtg
     5701 tggcgggttt tatcctgtat cacttattga aaaatccgtc caacgaagtg catctgttaa
     5761 aagaaagatt ccggatcgac tggccggtga tccataagat cttttatatt ggcattccca
     5821 gtggtattga aaacggtatt ttccagctgg gacgcgttat cgtggtgagc attatcgctg
     5881 gcttcggaac cgtgcagatc gctgccaacg gcgtggcgaa cagtctggac agtatgggct
     5941 gtatcgtggg tcaggccatg aatctggcca tgatcacggt gatcgggcgc tgcgtcggtg
     6001 ccggtgattc cggacaggta cggcactata cgaaaaaatt attgaagatc acgtatgttt
     6061 gtacgtttgt ggtaaacagt atcattctgc tgtgtctgcc ctggatcctg aaatgctacg
     6121 gattaagcac ggagaccacg cagttgtcct acatactggt catgatccac aatggcatgg
     6181 caatgttttt gtggccagca tcttttgtac tgccgaacat gcttcgtgcc tgcaatgacg
     6241 tgcgctttac catggtgctg tccatattct ctatgttcat gttccgcatc ggtttcagtt
     6301 atatcatcgg tgtgaacatg ggctacggag ccattggtgt ctggatcgcc atggtactgg
     6361 actggatctt ccgcgtgatc tgcttcgtga gcagatattt gtccggccac tggctgaaaa
     6421 cggccgagtt aaaggataac agatagtata gagcatgatt tttgcgccca aataggttca
     6481 cggatgaact gttatgaggt ataatgtggg aaagagggag gtatgtaata tgcaggtagt
     6541 attggaaaat gaagctttaa aggtaacgat caacagcttt ggcgcagaac ttgcaagtat
     6601 tcgggggaaa gagacagata ctgagtatct gtggaatgcg gatgcgaaat tctggaagag
     6661 aagtgcacca gtactgttcc cctttgtcgg aagcctgaaa aataaggaat atcattatga
     6721 aggaaagacc tattccatgg gacagcatgg ttttgccaga gatatggagt ttgccgtgga
     6781 cgtacagacg gctacggaag catggttttc cctgcattct aatgaagaaa caaaagcgaa
     6841 atacccgctg gagtttatcc tgaaaattgg atatgaatta gaaggaaaag acctgaaagt
     6901 tatctggcag gtagaaaacc cggacgaaaa agtcttgtat ttttctatcg gaggacaccc
     6961 ggcatttatg tgtcctgtag atgaaaaagg aaagcagtcg gagtattatt tcaagtttga
     7021 tacggacaag gatctgacgt atggtctcat cgcagatgat ggacagggac tgctaggaca
     7081 ggaagaggat gttctgccgg tggcagagga tggttacgca cagatcacaa agcacctgtt
     7141 tgaccgggat gccctgattg tggagaacca ccaggcttcc gtagtgagcc tgtgtacccc
     7201 ggacaaaaag ccttacctta ccgtaagctt tgacgctccg ttatttgggc tgtggtctcc
     7261 cgcaggcaag ggagcaccgt ttatctgcat cgagccctgg tacggcagat gcgaccggac
     7321 gaccttcgac ggcagcctgg aacagcggga gtatggcaat actctgcagg cgggcggggt
     7381 attccgcagg gagtacacca tcaccgtaga ataaatccgg aaacagcagc gatatgttag
     7441 gtatcgctgc tgtttttaag tggctcatct tatgaattat tcttgttctt gtaatggaaa
     7501 ttcggaatat gctcccatct gtcccggaaa taatgggcga tcagtttcgg acggcggtct
     7561 cgggtaaaga tgcctttccg gttgccgtct gcgcgcatac agccctgcag ggtggcataa
     7621 tcggcgaagt tccatgcctg ttccccgata aaaaagtctc ttccgtcgaa ggccttgctg
     7681 tacattttat aaagttctac ctgaaattct tcactccaca tttccggcgc acagttatgg
     7741 agcccggcga gagtatcggc gccgtattcg gtcatcatca cgggttttcc gatcttttcc
     7801 cagaaatcca gttccagatt ccaggcgtag gaagcagcct ccagatcacc agagagattg
     7861 taccatccgt aataacggtt gagacagact acatccatgg ttctggtcac aatgtccttc
     7921 tcgtaattat tctggcagca gacaaaggtc acggggcggt tctgaggatc cagccggtgg
     7981 gtaaattcat agagagaatg ccagtaatcg taggcggatt ccgggaaatg ctcggtatcc
     8041 ggttcgtttc ccatggacca catgacgatg cagggatgat ttttgtctcg cgcgatcaga
     8101 tcccgcagga cctgctcgtg atgctctctt atgggaaatg tcttataagg atcacagctc
     8161 tctccggcac cgatgcccac ggcgggcgtc tcgtcaataa taacgatacc ttctctgtca
     8221 cagagccggt agatctcctc cgcgtaaggg taatggctgg tgcggaagga gttggcatgg
     8281 atccagtgta gaagattaat atcctttaca tcgaggcaga gatccagtcc tcttccgcgg
     8341 aaaaaggaat cctcatgctt gctgaagccc ttgaaataaa aaggctcccc gttgatcaga
     8401 aactgcgttc ccttcacctc tacggtgcgg aaaccgaatt cttccgtata gcagtcctct
     8461 ccgtaatgaa cttccgcctt gtacaggtaa ggagtgccgg gataaggctg ccagagatgg
     8521 acctgcggga tccgaagctc tccctttcgg ccggtggctt ctgctttgca gttgctttcc
     8581 gcgtctatga tttttaaagt gacaggagcg tcactatcca tcccatccgt gagaatttcg
     8641 taatttaaga cgccggtgtc accttcgata gacgggacca gagtaatgtc tctgatgtag
     8701 gaggccggag tgctgtagat gcgtaccgga cggttgatgc ctgcaaaatt aaagaaatca
     8761 aaattcggaa gattttgctt ttttctccat agctttgcgg cttctacact gggaatgcct
     8821 ggattgtccg agccgaagaa ggcgatattg ccctcattgc cgatgggcag agtgctgtgg
     8881 ctgatccggt tgtccacagc aacaccgatg gtaaggtgtc catcctccgg aatcagatcc
     8941 gtgatctcta cttcaaaggg gagaaaacca cccttatgtg tcataatcag tttcccattg
     9001 atataaactt ttgcaacatg agttacggcg tcaaaacgca atacctgacg ctgtccgttg
     9061 cttccgcaca aaagagccgg gagcaccacg gttcgctcgt aataagccca accgtaatga
     9121 ttccgataag ccggatcatc cttctggtca ttataagaag ccggcacggc aatcaggtcc
     9181 atctcctgcg atccgctcca gagatcctcc ggctctgtct catctcctaa ttgaaattgc
     9241 catactcctc ccagatcgat caagctgcgg gaacggttca taattggata taacatggta
     9301 gtcctccttt tgccttgtgg actgtaattg cgcttattat agggcaggac aaaaggaaaa
     9361 tccatgtgat tatttttagg aagtatgtaa atttttatga atctggcaag tcagtaatta
     9421 atggtgggta ttcctgtttt tttgcaaggg cgcaaatagc gccccagcgc ctggatttaa
     9481 agtagaagag catggcaatc atgccggtaa taaagctggc ggaaacactg accatggcat
     9541 attcgccata gccggcatac atgatgagat aagcggcagg gattcggaca acccataata
     9601 gcagaatatt tacgatagtg ggatagcgaa tctctccgat accattgata aagcacataa
     9661 tgccattgaa cacggcgtag gcccaggtga atggcagaac aagataagta tatcgctgtg
     9721 caaaagccaa cacggccgga tcacggctga acaacatcag cagaggatgg caaaaagcag
     9781 tcacaagtaa tccccctgca agggtgacga tgccggaaca gagcggtatc tgccatgcac
     9841 cgcagcataa acgacggtag ttccgggcac caaggttctg cccggcaaac acagtagccg
     9901 cagaagaata tgaagtaatg gcaatattgg caatgccgct gactttaccg gcaacggagc
     9961 atccggctac gaaggtggag cccagtgtgt tataaagagc ctgaagcagc acatgaccga
    10021 cagaataaag tgcggtattc agtccgagcg gcaagccgat ctttaacatt tcatgaagaa
    10081 taggaaggtc aaaaccatgc gcaaaaacag gaaaattcat ggcaggataa gtatgacgga
    10141 tgtagaaaat gctgaacatc caggacagaa acagtgaggc agcagttgcg atggcaacac
    10201 cggtgacgcc cattcccaaa ccggcagtca gtacaaggtc cagaacaata ttggtaacgc
    10261 aggaaatgcc aagaaataac agcgaggaag tactgtttcc cagacctcgt aaaatacctg
    10321 cgttaaagtt atatcccata ttgccgagaa tacctaacgt tgtgatacgc agatacacaa
    10381 tggcataggg catggcatcg gcaggaacct tcatcagcct gagaaggaac ggtccggcag
    10441 ctacgcctaa cagcgtaagc ggcagactgg cagcgtacag aaaaaacact gcggtttgtg
    10501 caagcttacg cagttgatcg gcatcggagg cacctgccgc ttgtgaaatc aggatggata
    10561 ttccggaacc aatgccaaga aagatacaca aaagaatctc cagtggctgg gaacttgtgg
    10621 ctacagcggc caaagcggat gcatcacaga aattgccgat gaccaaagta tcaacggttc
    10681 cgtataactg ttgtaagata tttccgatgc agattggcaa tgcaaactgt gctgcgcggc
    10741 ctaaaataga accttgtgtc atatcgattt tactcatatt cattgacaat cacccgcttt
    10801 gtaaaaatgg taaatattta aattgtagaa tgtaaaaatt catcagctta tactggcttg
    10861 gttcagagtc tatgccaaaa ctgtgtccgg ggttttatgt tgagaaagct ttcgatgctg
    10921 ccggttatat tctccattgt aatataatca tgatgccagg gtcaaaaggt ctaagcccac
    10981 atgagcttgc tcataggtgg gtga
//
LOCUS       DAWDHD010000077        10959 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_370_length_10959_cov_15.004494, whole genome shotgun sequence.
ACCESSION   DAWDHD010000077 DAWDHD010000000
VERSION     DAWDHD010000077.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 10959)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10959)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10959
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_370_length_10959_cov_15.004494"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3145 a         2355 c         2819 g         2640 t
ORIGIN      
        1 ctttccgaag aggagttttc ctggctcagg ggctgacaga cgagagcggg agcatgatct
       61 tcggaaccgt gacagagaat gtgggacagc agatggaaaa taccgaggat attcaggtga
      121 tcctggcaga cagacaggaa gtacttcgta tcctgcggga agaacgggtg tccatgcgct
      181 gtggactgat gctgatgcag tttttgaaga ccggggcaga agatcccttt gtctttttgc
      241 agttgtgacg gacgctggga gaaagcacag aggatagacg ggactgttac ccgggaaaaa
      301 caggaaagaa agacgttgat cttgcaaaag cagttcccag ttttagaaaa cttttttgag
      361 aaaagaggta aattatggca atcaaatata tagaacagga cagaaccttc tggttggaca
      421 cggaacacac cagctatctt ctagcaattg tagatcagga gaattttgtg ggacacgtat
      481 attacggtca gaaattgcag tataccgaga atacaccggc accggtatat ttgctgcgta
      541 cgggagaagc acccttcgtt ccgtcacaga ataacagaga gcgtgtgtca tttctggatt
      601 ccttccccat ggaatatccg ggaaacggtc tgggagatta cagagaaagc gccatttccg
      661 tgcggacagc acagggacat gtaggagtgc agttacagta tgtctctcat gagatcgtaa
      721 aggagaagcc ggcactgccg ggacttcctt ccacatttcc gggggatgag gactgcgata
      781 ccctgatcct acatctggcg gatccggtca tcggtctggc tgcagacctg atctatacta
      841 catttgaaga ggaagaggtg atcacccgca gtgtgatcct gaaaaataca gcagaacagc
      901 cgatttatct gacgaaagtt atgtctgcct gcctggatct ggattgcacg gatgaaaagt
      961 atgacatcct taccttgcac ggttcctggg ggagagaacg tcagatggag aggagaagcc
     1021 tgatgcatgg taagcagagc gttgggtctg tcagaggaga atcctcccac caggagcatc
     1081 cgtttattgc attgctgtca gaaaatgcga cgcaggatac aggtgaagtc tacggcatgc
     1141 attttgtata ctccggcaat ttcctggcgc aggcggagct ttcccagttc gattctatcc
     1201 gtatgactat gggcattcat ccggagaact ttgtctggaa actggagcag ggcgagagct
     1261 ttgcggctcc ggaagtcgtt atgacctatt ctgcggaagg tctgggaggc atgacccgcc
     1321 attaccacga tatgtaccgc aatcatctga tccgcggcga gtaccgtgat aaaaagagac
     1381 caatcctgat caacaactgg gaagctacct attttgattt taacacggaa aaactactga
     1441 agattgcaga acaggcatca aagctcggca ttgagatgct ggtcatggat gacggctggt
     1501 tcggacaccg taacgatgac agtaccagct taggtgactg gaaggtcaat gagcagaaac
     1561 tgccgggggg cttaaaatct ctggtggagc aggtcaatgc actgggaatg aagtttggta
     1621 tctggtttga gccggagatg atctccccgg attcagagct gtacagagag catcccgact
     1681 gggccatcgc tattccggga cgggccggca gcttatgccg gaaccagtat gtgctggatc
     1741 tgtcccgcaa ggaagtcagg gatcatgcat ttgaaagcgt ggcggcagtt ctgcacagtg
     1801 ccaacatcga atatgtaaaa tgggatatga accggcagct gtcggatctg ggaagcgtgg
     1861 aactgcccgc agaccgtcag ggagagctct atcacagata tgtgttgggt gtgtatgaac
     1921 tgcaggacag actgttgcag gaattcccgc atctgcttct ggagaactgc tccggcggcg
     1981 gagcgagatt tgatccgggt atgttatatt acagcccgca gatctggtgc tccgatgata
     2041 cggatgccat tgagcgattg aaaattcagg agggcacgat gttattgtat ccgctgtcca
     2101 ccatgggtgc ccatgtatcg gactgcccca accacacggt gggaagagtc acacccttcg
     2161 agaccagagg ccatgtggca ctggcgggca ccttcggcta cgaactggat atcacgaaga
     2221 tcccggagga agaccgacag cagatccccg cacagaccgc catgtatcac aaatacaacg
     2281 atctgatccg cagaggggat tattcccgga ttgcatcatt tttggaaaac ggtaattatg
     2341 actgctatca ggtgacatcc aaggatcaga aagaagcgtt ggtaacctat gtacaggtac
     2401 tttcccgacc gaacttccac agtaggagag tacgtctgaa aggcctggca ccggagaaac
     2461 agtatcgcat cgaggagacc ggggaaatct ttggcggcga cgtgctgatg caggcaggac
     2521 tgctggtgac acctctgtgg ggagactacc gcagcaggct gatccacctg accgaggtgt
     2581 aaaattaagg cagtgacatg ccttgacaaa cgatttttag aaatccaata tggaagtata
     2641 attgacgatt tatcgggaaa tgtgtagaaa aatagcgata aggagtacaa gattatggca
     2701 aaagcagtaa aattagcaga tatcgcagag agagttggag tcagcacagt aacagtgtcc
     2761 aaggcactgt ccggacaaaa aggcgtcagc gaggaagtcc gggagaagat ccgcagcatt
     2821 gcagaggaac tgggttatca gcagccgtca gcggcaaaaa aaagtcagaa ccagaagagc
     2881 tacaatatcg gtattctgat ctctgagaga ttccttgata aatatgaatc gttttactgg
     2941 cagatgtacc aggcggtagc taccagagcc acggcaaagg aatgctttac catgctggag
     3001 gtcatcggaa tgtcagagga agagaacggc agaatgccga agctggtaca ggaacgcaag
     3061 gtggatggca ttatcgttat cggtaagatg atggatactt acctgcagca tctgaataca
     3121 gaagccggga tcccggtgat ctatctggat tattataacg gaagggaagc cagtgattct
     3181 gtgatctcca acagctacta cggaacctat gagctgacct actatctgta tcggatggga
     3241 catcgcaaaa ttgcttatgt gggaacttta cttgccaccg agagtattac agatcgttat
     3301 ttcggttacc agaaagcatt gctggaactg gggctggaac agaagaagga atgggtgctg
     3361 gatgatcgtc atattgagac cagcaggatc gatacggtga atatgttgca gcttccgaag
     3421 gatatgccta ccgcattcgt atgcaactgc gatctgaccg caagctttct gatcaagaaa
     3481 ctgagagaga acggatatcg tgtaccggag gacatctcag tggtcggatt tgataattac
     3541 ctgtatcccg gactcagtga tatccgtatt actacttatg aagtagatat gggagagatg
     3601 gcgagaagag ctattcataa tatgatcaag aagatcacca gtgataattt caaaggggga
     3661 gtcaccgtgg tggaaggccg tctggtactg aaggacagcg tgtctcacgt atagactgcg
     3721 gcgcattttg ccggtagaca aagggcgtga caccggtgtg ctttttaaac aggtggatga
     3781 aatggttggt gttgtcaatg cctaccgcag agcccacctc atgaatcgga tcatccgtag
     3841 ataggagcag cgatatggct gcttctattt ttttctcttg cagatattgc agcggggaag
     3901 tatggaatgt agcggaaaat tcccgacaca gacggtagcg gctgatgccc aaagtctgct
     3961 caaagtattc taaagagtga ttttcagcat agttttcctc aaaatccgct ttcatggaca
     4021 gcagataagc aggaatgggc ttcacatccc gggagacaga cagggcggac agcatcagtt
     4081 ctgcgaacat gtcggtcagc tcccggtgaa gctgtatctg ctctgcagga gtcgggatga
     4141 atgaaaattt ggtcagctgc tgcatccgct gtgaaacgca ggagccgatg ggaatcgggt
     4201 aacaacaggt aaaagcagcc tgaaatatgc tataataaga agaaagctcc gctccactta
     4261 agaaaaacat ttgaaaacgc cactttccct gcagcagtcg gagggagaag ccctgactgc
     4321 aatctaagaa caacagatgc tcgtgatcca ggaccacctc actgtcccgg gtggtaagga
     4381 gagcactacc ctctaaggtg tagagcagca gataagaagg aaatgggttc cattcataat
     4441 aaaagggatc tgacaggcgg aattctccac cggccagagg cagtaaaaag cctttttctt
     4501 ccagggatac tttgtagatc tgtcccagga ggatctccgg ggaacgctgc ccctccacat
     4561 cggaggaagt gtgcttcaat gcattgagaa aagtggtgga aatgttcata caaaaacctt
     4621 gcctttccgc aatcttcaaa cttcggctgc tttagcagct tgtgcaggaa caaaacttca
     4681 tgtgaaaaaa atatttttca cactagaaac atgctttttg aaaaaaataa ttaagcttaa
     4741 atacattata agccattatt taagctgttt caatgacaaa ggatgcaaag aaaacaaaat
     4801 tagttaatta aatcagctaa tttaactcaa attaattact ttaattgtat aatatgtcca
     4861 aaacataggc atagaacttg tgaaaataga agataataac gaaaatagca agaataagag
     4921 attaaaaaca agaatagttg atgattaatt aatatgatta tgtataatta aattaagtta
     4981 caaaacagta aacgctatca aatgtaacaa acaatattaa ggaggatttc attatgaaga
     5041 tgaaaaagtt tttagctctt tccacagcat cagtcatggc actgtctatg gcagcatgtg
     5101 gaagcacaga caacggcggc gccgctgaga gttctgcagc aagcaccact cccgcaactg
     5161 cagaagcttc cagcgaggct gcagcacctg cagaggttac cagcctgaat tacgcagaca
     5221 ttactctggg tgaggattac accgacatta ccacgaccat taaggtcctg actaaccgta
     5281 ccgacctgtt agaggacggc gcagcagttc cttaccagac ttacattgat gcattcaacg
     5341 agatgtatcc caacatcact gttgatattg aaggtatcac cgactatgca agtgatacgc
     5401 tgcttcgtct gcagggcggc gactggggcg atgttatgat gatccccgca gttgacaagg
     5461 ctgacttgag tacatacttc cttcctcttg gaactactac cgagatggat ggtcagttaa
     5521 actactacaa tcagatgtac gaaggcactg tatacggtgt tccttacact gcaggtgtta
     5581 acggtggtat cgtatataac aaagctgtgt ttgaagcagc aggaatcacc gagatgccta
     5641 agactcccga agatttcatc gcagcattaa agcagatcaa agagtatgac agctctatca
     5701 ttcctatgta caccaactac gcagcaggct gggcaatggg cggccagtgg gatcccgcta
     5761 tcagcggcag tgctaccggt gacagcacct acatgaacca gaagttactg cacaccgcta
     5821 atccttttgc tgatcccggt gacggaaccg gcgcttacaa tgtatacaag gtaatgtacg
     5881 aggcagttgc tgaaggcctt accgaggaag actactccac caccgactgg gaaggttgta
     5941 agggtatgat caacaatggc cagatcgcat gcatggttct tggttcctgg gcagtgcctc
     6001 agatgaagga agcaggcgac aatgctgacg acatcgctta catgcctttc cctattactt
     6061 tagctagcgg taagcaggca gcttccgtag gccctgacta cagctttggt atcaatgcag
     6121 atgcatctga agagaaccag gcagcagcac tgtgcttcgt gaaattcatg gcagaagaat
     6181 ccggcttctc ctatgactgc ggcggacttc ccgttgccat caaggatacc agattaccgg
     6241 atttctatgc agcattcaag ggtatcgact tcgtagtgga tgagcctgcg atcgaaggtg
     6301 aagaggatct gttaaacgag ctgaacgctg aaagtggtct taacatcaac aacaacggtg
     6361 agaccaagat gcaggagctg gtagagtgtg cagcagatgg ctccaagacc tacgatgaga
     6421 tcatcgacga gtggaacgaa gagtggactt ccgcacagga gagctgtggt gtagagatcc
     6481 agtaatttca tcaagcagta aacattaagc gactaactta atcttctgaa gtaagaggga
     6541 accttttatc cggttccctc ttatctaaga gagaagggta tggaggtatt tcgatgagtg
     6601 cagagacaat gaagaaaaca aaaaaaccct tcagcatgaa aaaatgggta aagagcagga
     6661 acggacaaca gaccatcgtc atactgtcct tcatgatcat tcccctgctt ttgctgttcg
     6721 tgttcaccta tcttcctttt ggagagatgg taaaattcag tttttataaa atgaaatatg
     6781 ctaccccggt agacaaaaga cagtttgtag gattccggaa ttacattgat gtatttaaga
     6841 aagatgaatg cttcagcgca ttgaagctga gcctttatta catggcgggc gcaatggtgc
     6901 agctggcact tgcattgtac ctggcaacga tccttagctt taagaccaag tttggcaata
     6961 tgttcaaggg attcatgttt ttcccctatc tgatcagtgg tattgccatt ggatttatct
     7021 tcaaattctt ctacaccaga ggatttgtat tcgatacggt attacagtgg tgtggattta
     7081 acttagaaag tcttccgttc tggctgaagg acaagagcgt taacaatatc tcgctggtgg
     7141 cttcctcggt atggcgttat tacggcaaca acatggtact cttcatcggg gccatcatgt
     7201 ccgtggactc cgagatgtac gaggcagcag agctggacgg tgccaataaa ttccaacagt
     7261 ttatacatat tatcttaccc agcattaaga ccatcgttac gctgaacgtg atcctatcta
     7321 tcacaggttc cctgagtgct ttcgatcctc cctacgttat caccaagggt ggcggcggaa
     7381 ccgcaaccta cttcgtattg atggacaaga tcgcacatac caaccagcag gtaggtcttg
     7441 cgtctgccat ggctgtggta ctgttactga tcatttttgc agcaacaatt ttacaaaagc
     7501 ttttcttcaa actggtattt agaagctccg atgcggataa tgaagaatat accagaagaa
     7561 aacgtctcag aagagagaga agatggaaga aggaggcagt gtaagatggc aaagatgaaa
     7621 accaaccaca aacattacag cgcaggcgct gtcatctgga tcgttattaa gtatttcagc
     7681 ctggtattct tctctttcgt ggcacttttg cccattgtat cctgtgtcat cacagcattc
     7741 aagaccgacg cagaatatca gagcactaac gttatgaccc tgccggaaag ctggctgaac
     7801 ttccagaact ttattgacgc ttttaaccgt gccaacatgg gcagagcata tctgaactca
     7861 gcgatcatcc tggtatttgt actgttagga tccatcctgg tgggaacaca gcttgcttat
     7921 gtactgaacc gattcaagtt cccaggcaac ggactggtcc gcagtctgtt cctgtttgca
     7981 actctccttc ccggaattgc catgcaggtc tctgtatacc agatcatgag taaccttgga
     8041 tttatcaatc atctgtatgg ttacatcatt atgagtatgg gtacggatgt tatctccatc
     8101 tacatcttca tccagttcat ggaaaatatt tcggtatcgc tggatgaatc cgcatttatt
     8161 gacggagcct cctactttac catttactgg aagatcattc ttccgcttct gaaacccgct
     8221 atcgtgactt cgatggtgct taagggagta ggcgtatata acgagtatta ttgcgccagt
     8281 ctgtatctgc aggacaagag gactctcggt gtggtagcaa cctccctgta tactttcacc
     8341 ggacctcttg gaagcaaata caatctgatc tgtgcaggtg tactgatgtc tctgctgcct
     8401 gccctgatcg tattcctgct ctgtcagaaa cagatctaca gtggaatcac ctccggcgct
     8461 gtcaaaggtt aaattcaaag gagagtgcgg aacatgaaaa atgcggaaac catgaaaatg
     8521 agagaagaga tcaaaaagca tctgaccgaa ggaatcattc ctttctggaa agggatgcgg
     8581 gatgacgagt tcggcggtta ttacggtttt ctggactacg atctgaatct ggataaaaag
     8641 gcggaaaaag gctgcatcct gaacagcagg atcacctggt tcttctccaa cgcatataca
     8701 ctgttaaaag acgaaagtct gttggatgag gcaaagcacg gctacgattt cctgaaagac
     8761 cattgtctgg acaaagaata cggcggtatc tactggtccc taaactatga cggaaccccg
     8821 aaggatacca caaaacatac ttacaatcag gcgttctgca tctacgcgct gtcctcttac
     8881 tatgaggcca gcggggatgc agaagccctg gagctggcga aaaagctgtt tactctgatt
     8941 gagactacgt gtatggatga ggtgggatat ctggaagcat tcaccagaga ctttaaaccg
     9001 gaatccaatg agaaattgtc ggagaacggt gtcctggcag acaagaccat gaataccctg
     9061 cttcatgttt ttgaagctta tacggagctg taccgggtat ccggggatga aaaagtgcgc
     9121 agacgccttc tgtggatcat ggatgtgttc gctgagaaag tttataatcc gaagctccac
     9181 agacaggaag tgttttttga taagcattac aacaccattc tggatctgca ttcctacggt
     9241 cacgacatcg agactgcatg gctgatcgac agaggctgca aggttctgga tgatccggaa
     9301 ctgacgcaga agatgtatgt catcaccgga gaccttaccg cacagatcta taaggtggca
     9361 ttcgacggac actctctggc caatgaatgt gacagaggtg tcgtgaatgt acacagagtc
     9421 tggtgggtac aggcggagac cgtgatcggt ttcttaaacg actatgagaa agagccggag
     9481 aaaacggaat atctggaagc cgcagagaag gaatgggcat ttatccgtga tcatgtgatc
     9541 gataagagac ccggttccga atggttctgg gaagtggatc cggaaggaaa tccctatcct
     9601 gaccgcccca ttgtggagcc ttggaaatgc ccttaccata acggcagaat gtgcatggag
     9661 gttatgaagc gtattcccga ataaaactgt aacgggagac ctccaggagg tcatgaaacg
     9721 tattccagaa taattgattt attttaagag aggataccta atatgttaga gcgttattac
     9781 atagaaaaag agaagcagga gaagctgctt tcccggaaaa atgtaaagtc tgatttctac
     9841 aacggtattt atgaccgtta tgaatatccg gttctgacca gagaacatat tcccctgacc
     9901 tggagatatg atctcaatcc caagacaaac ccctatttca tggagcgtct gggtatcaat
     9961 gcagtcatga attccggagc gattgaatta aacggtaagt attatctgat agcccgtatc
    10021 gagggaaatg acagaaagtc cttcttcggc gtggcggaga gtgacaacgg tgtcgatgga
    10081 ttccgtttct gggattatcc catcttgtta gatgacacct gcccggaaga gaccaatgtc
    10141 tatgacatgc gtctcaccaa acatgaagat ggttacatct acggtgtgtt ctgttccgaa
    10201 agcaaggata cttccgtaaa tgatctgtcc gcagcagtag cggctgcagg cattgtaaga
    10261 accaaagact taaagcactg ggagagactg gagaatttaa agacccttcg ttctccccag
    10321 cagagaaatg tagtgctgca tccggaattc gtggatggca agtatgcttt ctacaccaga
    10381 ccgatggatg atttcatcga gaccggaagt ggcggaggaa ttggtttcgg actgtgtgac
    10441 gatatcgctc atgcagtgat cgacgaagag cgcatgacca gtctgcgtaa atatcacacc
    10501 attaccgagg ccaaaaatgg tgcgggcgct acccccatca aaacagacag aggttggata
    10561 catatcgcac atggagtacg taataccgca gctggtttac gttacgtcat ttatgctttt
    10621 gcaacggatc tgaaagatcc tgcaaaagtg atcgcagagc cttcgggtct gctgatcggt
    10681 cccagagggg aagagagagt gggagatgtt tcaaacgtag tatttaccaa cggtgctatc
    10741 gcgaatgaga agggggaagt ctttatctac tacgcatcca gcgatacccg catgcatgtg
    10801 gcaactacta ccatagataa gcttgtggat tatgtattca acaccccttc cgatccagga
    10861 agaagcgtgg agtgtgtggc acagagatgt gaactcatcc gcagaaatga agagttctta
    10921 aaacaggaag caagataaat aatacagaaa tgaggtaac
//
LOCUS       DAWDHD010000078        10869 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_374_length_10869_cov_12.372758, whole genome shotgun sequence.
ACCESSION   DAWDHD010000078 DAWDHD010000000
VERSION     DAWDHD010000078.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 10869)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10869)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10869
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_374_length_10869_cov_12.372758"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1628..1727
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2496 a         3013 c         2200 g         3060 t
ORIGIN      
        1 gttcgtcaga ttgcaggaat tcccgcaatt cccattgcca cacataccag ccacaagagc
       61 agcagatgcg caggatagaa taagtaaaag aaatatttat tctgccgccc tctcgttccg
      121 ttgtagaacc agatcagaat aaaggaaagt gctgcaaaca tctccaggct gtccatcaat
      181 aaaaaaatga tgacaccggc ccacatctgt gctttctttc ggttacggaa cagataaagt
      241 acagtgattg ccaaaatccc cttaaaatca taatccgtct taagaaccaa gcccagtaca
      301 catcctgtca gaataataac ggcattcagt cccacgcgca gaatcgtttt tccaaccgtt
      361 gtacccacaa aatgctgttc caccagatga cgacccgcga tcaccagcag ccccaataac
      421 acagtaaaaa atacattttg atatgccgga taccatgcct tgccgctgaa tgccagatca
      481 aagggtacct ctgaaatcag tgcaaataaa aacatcctgc ccagatactt tttcacattg
      541 tgggtctttt gaaatccctc taccagtaaa aaacagtaaa tggggaacgc aaatctgccg
      601 atgatacgca ttgccatgta gatgccgtac attcggccgt tctgccccat ccaggcagta
      661 taggcctcct gattcgccat agcttcatac agcccgttcc ggatcaacag ccttgtcaca
      721 atcacagccc cgatgtgatc gatcaacata gtcactaccg cgatattttt cagcactgca
      781 ctgctgattc cacgtttttc ctgtattttc ccttccgata ttacttctgt ctgcatcctt
      841 atattccatc ctcggttttt gtattattta attagtacaa taatgtattt gttctgcttt
      901 gtcaagcaac ccatttgtca gactcctaca aaaatgtccc ctgctgacgc agaggacaca
      961 cttattaaca atcttttttc atcctacctt atatccggtt ccccatacca ccttcaggta
     1021 tctgggctcc ttgggattgc tctcgatctt ctcacggatg tggcggatat gcaccgcaat
     1081 agtattatcc gctccgatag cctgcatatg ccagatggat tcgtagatct ggctgatgga
     1141 cagtaccttg ccgcgctcct gcactaggag ccttaaaatc ttatactcga tgggagtcat
     1201 cttcacttcc cgtccctcta ccgtcactgt gcggttcctg tcgttgatct ccagtccatc
     1261 cacgcggtaa atactatcaa tactttcgca cagattgacc agctgtgtat atctgcgaag
     1321 ctgggatttc actctggcta acagctccag cggattatat cccactgtca cataatcatc
     1381 tgccccggca ttcagtgcca tgatcttggc agtctccgca tcctgtgcgg aaatgatgat
     1441 aatggggatc gtggtcatat tccggatatc atgaatcatc tcaatcccct ctccccaacc
     1501 tttgccatcc agttccacat ccagcagcag aagctggatc tcctgtgccc gcagtaattc
     1561 ttgtaactgt gctctccccg tcactgccac catttttaag ccttctcctg cgagaaggga
     1621 actcattnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnacc ctacaatata
     1741 ctagacgtaa ttgtagggca aaaagttgca gtatttttat ttttcaggtg taaaggtttc
     1801 aggtgtaaaa aacttcttcc agtaccaggc ctttggccgg caccagcgct cccgccttcg
     1861 ctctgtctcc ggacttcagt acatccgtca cattttccgg tgtctggatt ccctttccga
     1921 cctcgaggag ggttcccacg aggatacgga tcatatgatg taaaaatcca tttccggtaa
     1981 atacaaactc cacacggttc gctatccttt cgatccggat ctcgtcgatc cggcggacgg
     2041 tggatttctt actttttttt gcagaggtaa atgccttgaa atcgtgctct ccaagcaatt
     2101 gctccgctgc ctttcgcata gcatctaaat ctaattcttc cggttcctct gtcgcatagc
     2161 gtctccagaa tacatccggg atctggctgt tgatgatgcg gtaacggtaa cgcttcccgg
     2221 tggcattgag tctgctgtgg aacctgggtg ccgcctccga cacctccacc acggcaatat
     2281 cctctggcag ataccggtta caataggcca tgatctcttc tgtactcatg tcagattccg
     2341 tatggaaatt cgccacctgt cccaacgcat gcactcctgc atccgttctg ccgctggctg
     2401 cgatctccac aggctccccg cacattttcg tcaaaagaac ttccattttt ccctgaatcg
     2461 tattgtctgt ggatgtctgt ttctgccacc cctgatatct ggttccttca tattgcagtc
     2521 gcagtctgat atttctcatg atttcttttc tttctgtttt ttcgcaaaag cttcttttcg
     2581 agcttctttt tgggcttctt tttgagcctc ttcctctgct gcctgtctgg ccagttcctc
     2641 gctcagcctc tggtctctct cctgcatctg ccgcacttcg tccatatcct ctggcttcag
     2701 tctgcggaaa tgattgattc cggctgtcag gatcttcaca ctgtccttag tcgtctgtaa
     2761 agctccctcc tgatacatgg tcagagcgat cagaccaatg ggcactgcca cgatcattcc
     2821 tacgacaccg cccagcttat atcctatgta cagaagaaac agggtcggca gaggcggcat
     2881 tcccacagaa tctcccacga tcttcggctg gatcagctga cgtgccaact gtccgatgcc
     2941 ccagatgatc agcagcccta tagccatttt ataatcagag cttaagatct tgataattgc
     3001 ccaggggacc atgacggttc cggttccaaa gaaaggcagg aaatccaaaa atgcgattcc
     3061 cagtgcgatc agcaacgcat agtctacctg cagaacagca aatccgatga ctaatagtag
     3121 atacatccac acttcaatct taagctgtgc cttaagatat ccgcctactg ctttgaccag
     3181 tccacgcttc agcatataat accaggacag tacttccgcc ggtgtatgct tacggaacca
     3241 gactgccagt tggtctctct cagccacaaa gaaataggaa gaaagcagtg ccatgatcag
     3301 tccgatcagg atcgaaggaa cctgcttggc gaaattgccc acggcattga tcgtaggggt
     3361 gccgatctta cccagaagat ctcccacata gcttccgatc tgattgatga catccatgat
     3421 gctttgctgt acatccgcgg gaaaacggtt atagataaca ctcaggttct gtccgatatc
     3481 ggcaaaatca ttctccgcac tgtcccagat gtccggcagt gcaccgatca atcccatgat
     3541 ctcatcggtt aacttcgccc ctaccagata aaggatcaat atcaccagcg cgatgaccgc
     3601 cacgatgaca aatgcacttc ccgcttttcg cttgatcttt accttttcct caaaaaacct
     3661 caccagggga ctggcgatca acgcaatgat ccagcctgcc acaaacggtg cgaaaaatac
     3721 taacagcttc ggcaccagga aaacgatcaa cagcaatata ataaatgcca cacccagatt
     3781 cactaaggct ttacaatatt tcttcatata ctctcactct ttcacgcatt ctgcttttac
     3841 acctattcat ccttcctgct gtctattcct gtgcaggagc ctctgctgcc agcactccgt
     3901 ccaggatctc gtagatttct tcccttccct gtttcgtctg tgcggaataa ggtacgatct
     3961 gtgtatccgc tccggcctgc agggtggtgc ggatcagctt cacctgcttc tgtatctgac
     4021 tgcggttgat cttgtccagc ttggtggcaa tgatgatggg atcgtagcca ttgcgacgga
     4081 tccagtcata catgatccgg tcgttctctg agggagcatg ccggatatca atcagcagaa
     4141 ataccagctt cagctgtttg gatttatgca gataatcttc gatcatctta ccccactggg
     4201 ctttcacatg ctcattggcc tgtgcatagc cgtagccagg cagatctaca aagtagattt
     4261 catcgttaat gttgtaataa ttgatggtct gtgtcttgcc cggctgggaa ctggtcctgg
     4321 ccagagactt acggttcatc aatccgttga tcagagagga tttgccgaca ttacttttcc
     4381 cggcgaatgc cacttcggga tgtatattct ccggaagctt gctggtaata ccacagaccg
     4441 tttccagact tacattcttt atgatcataa aaactccatt tctgcgctgc ttttgcgcat
     4501 cttaggtttg tacttttttg cacaaaacgc tgccctgtgt gaccacaagg cagcgcccgg
     4561 attattttgc ctttttcaga gattcatcat gtatggtgag gggctcgctc tctccatcca
     4621 ctgccgcctt ggtgacaaca cattcttcga tggtgtcatc cgaaggaatc tcatacatgg
     4681 tatccatcat tacggattcc ataatggagc gtaagcctct ggctccggtc ttgcgttcca
     4741 gtgccttgtc agcaatagcc tcgatggcat cctgctcaaa gctcaattct acgttatcca
     4801 tctggaacag cttttggtac tgttttacca gagcgttctt cggctcttga agaatacgga
     4861 tcagtgcttc tctgtccagt cctcttaagg agacacagat aggcacacgt cctacgaact
     4921 cggggatcag accgaacttc accagatcat gaggagttac ttcctgcagc agttcgctga
     4981 tatccttatc ctctcttctg cccagctccg tattgaaacc gatggcctta cgatccagtc
     5041 tggactctac gatcttctcc agtccatcga aagcacctcc gcagataaac agaatgttgg
     5101 tggtatcgat ctgaatcagc tcctgatgag gatgctttct gcctccctgg ggaggtacgc
     5161 ttgccacagt accctcgatg atttttaaca gagcctgctg tacaccttct ccggatacat
     5221 cacgggtaat ggatacattc tcgctctttt tcgtgatctt atcgatctcg tcaatatata
     5281 cgataccgta ctgtgcacgc tctacatcat aatctgccgc ctggatcagc tttaacagga
     5341 tgttctccac atcctcacct acataacccg cttcggtcag tgtggtggca tccgctatgg
     5401 caaacggtac attgatgatc ttggccagtg tctgggccag gtaagtctta ccggaaccgg
     5461 tagggcctac catgataata ttactctttt gcagctccac atcattcaga tccttgggag
     5521 ccgttactct tttgtaatga ttgtacaccg caacggagag gacttttttg gcctcctcct
     5581 gtccgatgac gtattcatcc aggaagttct tgatctcctt gggtttcaga aggttaatgt
     5641 cgggactcgc agtctcttcc acttcttcat cttccagttc ttcctccagg atgtctgcgc
     5701 agatgtctac gcactcatca cagatatata ccccggcagg tcccgcgatc agcttgcgga
     5761 cctgactctg ggtcttgtta caaaatgaac atctgatgtc atttccggaa ttttttcctg
     5821 ccattattat actcctgttg ttttatgcct cgtggaaggt gatcacctta tcgatcagac
     5881 catactcaca agcttcctca gcgcttttcc agttatctct ctcggtgtct gcgcagatgg
     5941 tctcataatc cttgccggtg ttctcagcca gcatacggtt caacttctcc ttggtacgga
     6001 gaatatgctt tgctgcgatc tcaatctcgg tagcctgacc ctgtgttcca cccagtggct
     6061 gatgaatcat aatttctgca ttgggcagtg caaaacgctt gcccttggca ccgcctgcta
     6121 aaaggaaagc tcccatgctg gctgccatac caatacatac ggtggacaca tcgcacttga
     6181 tatatttcat ggtatcataa attgccatac ctgcagtaac gcttccgccc gggctgttga
     6241 tatacaaatg gatatcctta tccggatcct ctgcttccag gaacagcagc tgtgctacaa
     6301 tgatgctggc agaagtctca ttgacttcct ctcccagaaa aatgattctg tccttcaaca
     6361 gtctggaata aatatcataa gaccgctcac ctttgctggt ctgttcaatg acgtaaggta
     6421 ctaaactcat attctaaaac ctccattact tctgattaat attactatcg tatcctactt
     6481 agcaggcttt ttcaacgaga ttccaaagat ttaataaaaa tttaaaagaa agcgtatccc
     6541 cacctccctg cggacgtggg aatccgcttt ctctttctat cgtatcttaa agcatatatt
     6601 atgcttcttc gggcttctct tctgctgcct tttccttctt ggtggtcttc ttggctgcag
     6661 cctttgcttc cttagcgttg tctacgatga aatcagctgc ctttcttaca cagatatctt
     6721 ccttcacctg cttagcgccc tgctcaccca gaagctcttt taccttgtca gcttccatct
     6781 ggtaagcttc tgccatgctc ttaacttcag cttcgaagtc ttcctcggta gcttccatct
     6841 tctcagcagc tgctacagct tccagaacca gacgggactg gattctcttc agtgcttcgg
     6901 gcttcagctg ctccatcatg gtctgagcgg tcagaccggt gaactgcatg tactgctcca
     6961 gagtgattcc ctgtgcctgg attctctgtg caaagttctc taacagctgt ctctgctggg
     7021 tctctaacat agcatcgggg atctccatct gagcgtctgc gatcactgca tccagaactg
     7081 cctcttcctt ggcaatctta gcttcctctt ccttcttgga agtcagcttc ttctgtacat
     7141 cttctctata ctcagacata gtatcgaaat cggatacctc gcttgcgaac tcgtcatcca
     7201 gatcaggaag ctgcttctcc ttgatctcct taacggtgca cttgaatact gcaggcttgc
     7261 ctgccagctc ggttgcatga taatcctcag ggaaggttac gtttacttcg gtctccttgt
     7321 tcagttcagc accaaccagt gcctcttcaa aaccgggaat gaaggaaccg gaaccgatgg
     7381 tcaggggata gttctcaccc ttgccgccct caaatgcaac accgtctaca aaaccttcga
     7441 aatcgataac ggtcatatcg ccgtccttaa cagcacggtc ttccacgctg atggttctgg
     7501 cattgctctc acgctctctg ttgatctcag cgtcgatatc ctcatcgctt accttaacgt
     7561 cgatcttgtc taccttcaca cccttgtatt tgccaagagt aacttcaggc ttcagagcaa
     7621 cctctgcggt aaagatgaaa ggcttgccgg cctctaactg ggtcacatcg atcttcggag
     7681 aagatacgat ctgctcctca cactcttcca gagccttatc gtaagcatcg ggaatcaatg
     7741 cgtttgctgc atcctcatag aatacggact tgccgtacat ctgctcgatc atcttacggg
     7801 gagctttgcc cttacggaaa ccgggaatgc tgatcttgtt cttattcttc tggtaagcac
     7861 cttcgattgc cttctccagt tcttcagcag atacttcgat ggtcagcttg gccatgtttt
     7921 tctctaactt ttcaacctgt aaactcatta taacatttcc tcctttatac ccttgcgggt
     7981 ctgtatccag tcgcagtcat tttgcaatta tagcataagt tggaggaatt gacaaccttt
     8041 ttattttggg gctttttgcg cgtccggggc atacagacac ttcgtgctga ctcccgggat
     8101 cattccaagc ttccccgaca gggcactgac tttttcctgg ggggcatcca tgaccacact
     8161 gatgatgttc atctgctttt catgataagg tatccccatt ctgccgatga tatattcccc
     8221 atattcgtgt aaaagtccgt tgatccttat cgccgcctcc ggatcctcca caataatgcc
     8281 gatcagggtc actcttgtat tttccgtacc tttttccata tcggtcttcc tcctgtggtt
     8341 tctatatctg catctgcgtc atttccgaaa attcttctcc ggttctgata tgagtagcta
     8401 tcatcacgat ccggcctctc gtcttcctcc ggatatagtc ttcggccttt ttgcggtttt
     8461 cttcatccag tccggtaaaa ggctcatcca gcagaacaca ctgcgctccc gcctccatgg
     8521 cccgtaccag agacactctc cgtttcatgc ctcccgatag ttcgtggcag ggctgatcca
     8581 acgcttcttc cggcagcaac tgcgtcaatg ccgttctcgc ctgtgaagca tctcccagga
     8641 tcatttccag attcttcacg gcactacagt cttcacacaa acgatcctcc tggaattgca
     8701 tggcaaaagc atccatttcc gacacttctc cctcctccgg ccgctccagt ccgcacagac
     8761 accggaaaaa agtggtcttg ccactgccgg aagggttatc cagtacatac ggttccccgc
     8821 tgtgaaattc gtgatccaca tgtcggaaaa tccattgatt gtgaaaactt tttccaagat
     8881 tccttacaca cagggtgcgc tgttctctac cagcgatttt ctgtggcatg gagggatcct
     8941 tacaggcagg ctcccaccgg aaaaagactt caatcccgta gaatattatt ttttcaaaaa
     9001 atacactgag tactacgatc accgctgtcc aggcaaacag atccgccgta tccagataaa
     9061 tcttcgccag atacagtgct ccaccgatgg aatgactggg agtgccaatc acttccgccg
     9121 ccactccgga cttccagcac attcccagag atagctgaaa cgcgctgagc aaaaaaggct
     9181 ttaacgccgg acggtaaatg tagaagaact gagtaccaag gggaaggcgg aatacctccg
     9241 ccatctctaa cagccctctg tccgcgctct ttagtccttc cagcgtattc aggtaaatat
     9301 ttggaaatac taccagaaaa cagatagcca ccgccagcat ggaagacccc caccagatca
     9361 gcaaaagcac tgcaaatacc gctaccggta ctgccttgca gaataccatg aaaggacgca
     9421 ggacttcttc cgccagcgga taacggtaac tgatggccgc cagaagaagt gccaataccg
     9481 cccccagcaa aaagccgaag gcgatccgcc acagcgaatc aaaggcactc cgccagaact
     9541 ccacctgccc ggccagagta agcagtgccc gcaatgcctg caaaggtgtc accaccagca
     9601 ggaaattgtc cacacacatt gccagaatct gccatactcc cagccagaat aacagtatta
     9661 taaatttttt aacgtatatt ttacccttat tctgcatcat aatagaaatc ggattccgga
     9721 agtccgccac ctatggacag ggaatcctgg tggaacagta cgtcaaggta tccggaaagt
     9781 gcctgcttca tttcagcttt gtcaatatag gtaatgttgc agttcggaat tgctttctga
     9841 gcaatgggct ctttggctac gatctgggct tccactgcca gtgcagcacc ggtggcaggg
     9901 tctgcattaa tgtcttccgc acttgcctta tgatcttcca taaaggattt cactgcttct
     9961 tcgttctcct cgaggaattc cttgcgaacc accgttacac cggtgaccat gctgctgccg
    10021 tcctctccct gtaccttatt ccactcttcg tttaagtcga agatcacttt cagtgcatca
    10081 ttctgcatgc aggctgcggt aacaaaaggc tggggcaaca ggccgatggc tgtgggatct
    10141 tctgccagta cagatgccac ctctgttgct tcggatttat actccagtat cacgtcatcc
    10201 gcagacatac catttgctgt cagcaggtac tgcaatacat agtccggtgt ggttcctttt
    10261 ccagtcagat aaatggtctt accctttaga tcggtaaagt ttgtcagacc gtcttctcct
    10321 gataccatat aaagtacacc cagagtattg atatcaatga cttccacacc gccctgtgtc
    10381 ttatgataga gaacgcttgc cacattggcg ggtaccagtg caatgtccag atctcccttt
    10441 accatcagcg gcaggatctc atctgcacca gtagccatct ggaattcata ggtattggca
    10501 gtctcaccct tgtctgcttt attcattaaa aataaaagtc ctaatgaggt cggtcccttt
    10561 aaggatgcca cacgtaccag cgtgccatcc gcggtctccg tattttctgc tgtggcttct
    10621 gcaacggttt ccgtactctc caccgtttca gtgggctgta cgctctccgc tgccagatct
    10681 tccgacactg tttccacact ctgtgcctgt ccgcatccgg caagaccgct gcacaacagt
    10741 ccaagagtca gtgcaagtgc cagagttttt gtgatttttg ttttcttttt cattgttttt
    10801 tcgcctttct gtcataaaat gatgtcgggg tcaaaagccc ccgactttga tgcctacgga
    10861 ttgttccgt
//
LOCUS       DAWDHD010000079        10693 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_380_length_10693_cov_11.973491, whole genome shotgun sequence.
ACCESSION   DAWDHD010000079 DAWDHD010000000
VERSION     DAWDHD010000079.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 10693)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10693)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10693
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_380_length_10693_cov_11.973491"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2272 a         3056 c         2281 g         3084 t
ORIGIN      
        1 ttcttcggta attccataca aaaaacacct cccatgccgt ttctatcagt tatcactaac
       61 tatagcatcc gcacaggagg tattttagcg ttctttgtcg cacctcttca aaaacagttc
      121 gacaaagttt gcaccatatt ctatgtattc ttagctatgc ttcaaactta tatacggtaa
      181 cagaatcgta ttctctgccg ttctccccgc caaatacata ggaggggaaa ttctcatgat
      241 gaatggtatc cgggaaatac tgggtctcca atgcaaaacc gcagcggttg ccgtaggtca
      301 ccccatcttt tccgggctgt acatcaataa aattgcctgc ataaaactgc actcctggca
      361 gggtggtgta tgccttcatt actctgccgc tcttcgggcc ttttactgtg gcaatatgcc
      421 gtaatgtacc gtcatcgttc catccatcga tcacgaaatt atggtcatag cctccggtca
      481 ggagtaactg ctcataatct tcccggatgt ctctgccgat cttcttgggc tgtgtaaaat
      541 ccatgggtgt gcctgctacc gcacgaatct caccggtagg gatcgatcct gctgccaccg
      601 gagtaaagtg agaggctctc agccacagtt catgctcttc aatgctgcct gcaccgtgac
      661 cgtccagatt gaaataggaa tggttggtca ggttgataat ggtcttcttg tcactggaag
      721 catggtaatg cagagtcagc tcattctctt cactcagaga gtaggttacg gataccttct
      781 tattgcctgg aaatcccagc tgcccatcct catcctccag agaaaatgtc acgctgttct
      841 ctcctatctc cgcatcccag agtctcttat ggaatcccag ttgcttatgg gtatgtaaat
      901 tgttggcacc gtcatttttg tccaactgat aagtaacgcc atccagcgta tactctgcac
      961 cgccggtacg gttcgcacag gggccgatca ccgtgccgaa aaagctgtcg ttgccgtaat
     1021 agctctcacc attgtcaaat cctagcacca ggtcgtccac cttgcccgtg gcatccggta
     1081 ccagtacgcg caccagagta gcgcccagat ccgttatctc tgctttcatc ccttgatcat
     1141 tactcagggt atacagagat atctctttcc cttccgcact tttcccaaat aaacttttta
     1201 ctactgccat tgtcttgtcc tccctctttt aactataatt ccactctgcc ctctaaagcg
     1261 cggagtaatg ttacctcatc aatatattcc agatctcctc ctacagggac accactggca
     1321 attctggtca ccttgatccc ggtcggtttg atcaatttgc tgatatacat tgccgtagtc
     1381 tcgccctcca ggctggaatt ggttgcgata atgacctcct ccacatcacc ctgcagacgt
     1441 tccatcagtt ccttcagctt gatgtcaccg ggtcccacac ccagcatcgg agaaatggca
     1501 ccatgcagta catggtacac gccctcatat ttgccggtct tctcataagc cgccagatct
     1561 ctggtgttct ccaccaccat aatggtacga tgatcccgtc tgggattcgc acacaccggg
     1621 cagagttcct tatccgtcag agtatagcat tccttgcaat agcggacatt ggctctggct
     1681 tcgatcatga cgtttgccag cctgtccacg cgttccttcg gcatattgat caggtgaaaa
     1741 gccagtcgtt gtgcggactt actgccaatc cccggaaggg ctgccagttc ttctattaat
     1801 ttattgatct gtccgctgta gagttccatt agaaaggaaa tcctcctaat cctccggtca
     1861 ttttgcccat aagctctgca ttggcttcat ccgccttgcg cagtgcttcg ttggctgctg
     1921 ccacgatagc atcctgcaga gtctcgatat cctcgggatc cacgatctcc tctgccagct
     1981 tgatggatgt gatctctttt ttaccgttga tggtcacttc cacagctccc ccacctgcgg
     2041 atgcggagaa ctccttggtc tccagttcct tctgtccttc ttccatctga cgctgcattc
     2101 tctgtgcctg cttcatcaga ttattcatat ttccgggcat tgcgcctccg ggaaatccac
     2161 ctctttttgc catgttgctg tttcctccat attatttata attgagttat tattctatct
     2221 taccttatct gcaagtctgc cgcaatccca aattcacgca ggatcactct tcctcatcga
     2281 tctccatgtt gatgacctta ctgagatcca catagttgtc ttcgaattct ctgctgcttt
     2341 ccaccgtctg gatcgtcact tcaatctctt tgctggaaaa atcggacaac agcatttcca
     2401 gctgctgctt gttctgctca tgagcttctc ctttgaaata atcggaagcc agtccgtcct
     2461 ccaacaccac catgagcttg ttgtctccgc ccagacttaa tcgggcattt ttcaggtaca
     2521 tcttcatggg gttctctgca tttccaacga tggccggcca tttccgtacg atctcctgga
     2581 tatcctcggg gaccgccttc ggcaactgtg gcttcggctt tgccacaccg gcctgcggca
     2641 ctccctgtgc tccgggagct ccggcaggca tttgcgccgc cgtaacgaca atgccgtttt
     2701 ccactttttc ctccacctgt cggatacgat ccaggacagc ttcctgatcc gtctccatct
     2761 ccggcttaca cagcttgatc agcgcgatct ctaccagaat acgtttctgc gccgcatagc
     2821 ggatctgtcc ggacagttcg gagaaaatat gaatatagcg gatgatctgg tccatggaca
     2881 gcatggaagc ttcttccttc agattcgcca gattgtctgt ggacatatct atgacatcct
     2941 ccagattatc cgctgtctgt accaacatca gattccgcag ataccaggta aagtccgtga
     3001 cgaactgtac cagttccctg ccctgcatga cgatctcttc caacagttca atgcagccta
     3061 acacattccg gtccatgacg aagcgcagca acctgctgaa tacttccgta tccactgccc
     3121 ctagcacatc cagggtcata tcgtaggtca gttctttgcc cagatggaag gcaatacact
     3181 gatccaacag gctcaaggca tcacgcatgg agccgtctgc caccttggca atgtagcgca
     3241 gagctctgtc ctccacctgt acctgctccg tgtccatcag ttccttcatg cgatccgtga
     3301 tggtatcaat ggagattcgt ttaaaatcat atctctggca gcgggacagg atcgtaatgg
     3361 ggatcttgtg cacctctgtg gtcgccagaa taaaaataac ataagatggc ggctcttcca
     3421 gcgtttttaa caatgcgttg aaagctccgg gggagagcat atgcacctcg tcgatgatgt
     3481 agaccttgta ttttccctcc gcaggggaat aggatacctc gtcgatgatc tcacggatat
     3541 tgtccacacc gttgttggaa gccgcatcga tctcgatgac attcatgctg gctcccgctg
     3601 cgattgcctt acaaatacgg cattccccac aggggccgtc ctctgtgggg tgttcgcagt
     3661 tcactgtttt cgcaaaaatc ttggccaccg tggtcttacc ggtacctctt gttccggtaa
     3721 acaggtacgc atggccgatc cggttcgccc gcagctgatt cttcaatgtc gtcacgatat
     3781 gctcctgacc cttgacatcg gaaaaagcgt ccgggcggaa tttccggtat agtgctgtat
     3841 atgacatgtt ttttctctct tccttgtaac tattcagagc catcaaatat tagcagatgc
     3901 ttggcgaaaa tagttactca ttaattttct gtcttaatcg ccgaagcaga tgcccagcag
     3961 cttcgcttcc tgtacgatgg tagcatcggg atccacggtc tttaacttgc ccgctacatc
     4021 ctctaacgga acccttacga tctcacggtt cttgtaacct accatgaatc catactgatt
     4081 atccaggatc agcttgcctg cctcggagcc cagacggctg gcgaatactc tgtcataagg
     4141 gcaggggctt ccgcctctct gcatatggcc gggtacggtg acacgtactt ctctgccggt
     4201 cttctcctgg atctgtgcag ccacttcgta agatacggaa gggaacggat atttttccat
     4261 ctttttcttg tagtcttttt tggtcagggc agcatcttcc ttggaaatcg caccctcagc
     4321 cacagcgata atggtaaaac ggcagccctt tttgtccctg tcttcgatct tctcgatcac
     4381 cttatcgata tcataaggga tctccgggag caggatgatg tccgcaccgc ctgccattcc
     4441 ggcattcagg gtcagccacc ctactttatg tcccataact tccacgataa atactctgcc
     4501 gtgggaagct gctgtggtat ggatgcagtc gatggcatcc gttgcaatat ccaccgcact
     4561 ctggaaaccg aaggtcatat cggtgcccca caggtcattg tcgatggtct tgggcagggt
     4621 cacgatattc agtccttcct tgcgcaacag gttcgcagtc ttgtgggtgc cattaccacc
     4681 gagaattacc aggcagtcca actgcagctt ataataattc tgcttcatgg cttcgacttt
     4741 attcagtccg ttgggatccg gatcctggat cgtcttgaag ggggtacggg aggttcccag
     4801 gatcgtgccg cccttggtaa ggatgccgga gaaatccttg tctgtcagca tgcggaaatt
     4861 accgtaaatc agtcctctgt agccatcgag gaagccatag atctcgacct cttccttact
     4921 gttcgccagg gtcttcacca cacctctcat ggcagcattt aatgcctggc agtcaccacc
     4981 gctcgtcaac attccgattc tcttcatcgt aggatcctcc gttttctata tttcattatt
     5041 ttctcttgcg tttgctgtaa ctatgcaaaa ctgtcttcgt tctgccaata cttatttcta
     5101 ttttaataca tttattttca tctgaaaata gtttgttgta atttccggca gatttctccc
     5161 ctatggcaaa acaaacctca gaagcaccgg gcctctgagg ttcgcttatc atcttatcat
     5221 acgttatttg aatttaaact ttcctttgat catggtcacg tagacagctg cgccgccacc
     5281 gccgaccact acggttccgg ctgctgccgc tgccgccaca cggcctgcgt tcgttccctt
     5341 accggaggtc tccgttatct gctctgtgcc ggattcctga gagccggaag tctgggcggt
     5401 catggaatct atccgctcct cctgcggtgc ttctccactc gattcctgcg tgtcctgtgc
     5461 ctccccggag ggctttgccg catcttcctt acggttttcg tccgcagcgg gtgttgtcgg
     5521 gggcacggga attacagaag gctttaccac accgggtgcg gcaggcgttg ccgtggctac
     5581 gggaggtgtt gccgcgtccg tggtcttctg gtccgtattg gacgcctgac tgctgtcatc
     5641 gtcgtcatca tcgtcatcat cgttactggc agcaggcttc ttcggtgcga tctcataacc
     5701 gcagatcgta cagatttccg cccgttctgt ggtggccgcg ccaccggatg catgcttttc
     5761 cgtattgctg gtgattccac agacaccaca cttatgccag tgtccggtca tatcggacag
     5821 atatccttct gcccagctat gatccgtctt cggcagcacg atttctgccg ctgttatgct
     5881 cttacttcca tctgcggatg caaaatacag gccgcagacg tcgcaggtcc aatactcgat
     5941 gctgccctct gcctgacagg tgggttccac ccggtcgtgg tgggtcagcg ggccgtgacc
     6001 ggagttccgt gtcttcgata cggcggggct tgcgttataa tcatcggttt ccgcatagcg
     6061 gatctgtgca atcaccttct cgttgtgacc gactccggtg gatttggtct gcccccagtc
     6121 cgttccatca aagctgaact ccacgtcagt gcggtctgtg gtgatctgcg ccgccagatc
     6181 gttgccgctg acggtaaagg tcatgtcaaa ggcatccgga tccggggtat cctcgcggtc
     6241 tgctctttcg gttcgatgta tggttatatt cacacagatg tctttataat tttgcgacct
     6301 caatacaatt tgaatagccg cagaatgctt gatatcctcc atggtgtttt ctcccaatgt
     6361 gaatatcagc ttaccttccg aatatctgat agcctggtct gttgccaaaa cgttagcatc
     6421 atctgacttt accatattga ttaattctgt cgttcccctg tcttccggca gtcccggtat
     6481 ctgtacttcc ttctctcctt tggctgccca actgtaatat aaattctgca ttggaattgc
     6541 cgaaacttcc gccttttcaa ttgccagctt caccggtgca gtcaacggct tatagtattc
     6601 cggatgctgt accggggtaa atgttgccgt gtaggttcca tcgtctccga cacaaggaac
     6661 ctcgtctccg atgatctgcc aggttccgtc gatgatctcg gttcccagtt ttgccgtcag
     6721 tcctgtcact gtgagttcac tcagcttatt gccataggtt ccacaggcaa tgccctcccc
     6781 ttctacagta agtggagccg gggcgatgtc aacgatcaga ttcttcgtct tctcagagaa
     6841 gaaagaattg tcatttcctt tgtagacgag ggtaaacgtg gtcgcacctg cagtccgcgc
     6901 attgtaggtt gcggtgccgt cccggaattc tgccgcattt accaccgggg tatcctcgat
     6961 ccggatctca aagctgccct ccgtatcttc cgcagagatg ttctttccgt ttgtcacaaa
     7021 gtttaccagt gcctttacat taaaagtaat atcttgtcca taggtgggaa tagcgttatc
     7081 tgttctctcg aacagcagac ccgtggtgta ctttacacca actgcgttat tctccccaag
     7141 agaagggtag taggcgattc ccttctcttt gtcgttggct ttctttgtcc agagttcggt
     7201 gttaaagtcg tatgttttga gttcttcgaa aaagctggaa gaggtcagtt gcagggtcgt
     7261 acgccccaaa ttgcgtttat catctgcttt acatatatcg ttattgaaat agcaattcgt
     7321 tgtaccgtaa tctccactgt tcactattcc tgcacaatct ttaccacttg taacagcagc
     7381 tatactaatg caatgatcaa taacttgaga tggactcttg ccacagattc ctgccgcaga
     7441 atcctttccc tgcacgtctc ctgcaaaaaa gcactgggaa attatagttt tttgtccggc
     7501 attgctgcag attccacctg ctgtataccc ttttaccgtc cctataaagg agcagccata
     7561 aatttcgcca ttaagcggat atccgcaaat tcctcctgcc tcgttattcg aagcaatcac
     7621 tccgttggca gccaaaagca ttactttacc gctttctgcg atctgaccat ttatgttacc
     7681 tatcccgcca aacaagccca gacaatcacc tgccatattg acatggccta tcgtatgtcc
     7741 gtctccactg aattttcctg tgtacggagt tcctggattt ccaatagatc tgagttccac
     7801 tccgctcaca tcaatatcgt tccccaggac atagtttgca ctccattttg tctcatccct
     7861 atcctgtccg atggctgcga attcctccgc cgtggtgatc agggtataga tatcccagtc
     7921 gtcgccattc gttgtaacat agttgtaaac cggtacggaa gcagtcttcg tctctccgat
     7981 ctctgccacc tttctcagat tgatgtaagt ccctgcggct gtaccgaatc tacttcctgt
     8041 acctgtagcc actgcagtgc tggtatcgac actgccctct ttccagattg taggatccaa
     8101 gcccttcgga agtgcaccgc aaagctggct ggtagtcagg ggaatcacct tatcgttttc
     8161 tccttctccc acaccggcat tattcaaatc actgttgtaa tagcagttgc cgataaagtt
     8221 catataactc gaatccgtgc ttccaactat gccaccaaca tttttgccat tccctgtaat
     8281 cttaccacaa ttcaggcaat tactgatgtt atacctattc gctgatgcac cggtaatacc
     8341 acctacagaa ctatcctgtt gagaaactgt tacagcacct atattataac agtattgaac
     8401 tacgccgttt ccatctcttc cttcataact ggcactgccc atgattccgc ctgctgtaga
     8461 atttcctttt actgaactgc tattgtagca gccggaaata tttcctatca gacttccgca
     8521 tacaccgcct acacaccgac tcgcataaat cgtgttttcg atactatagc agtcagaaat
     8581 atttgctcct gctccagcaa tcgcgcccac aaagctatga ccagaaatat tagcccgctt
     8641 tactgtgaca ctttttatat agctggaagg agcggtaaag ttgtagcacg ctccaaacag
     8701 acccacatat ttgttttcct ttcctgtaat tgtcattccg gttattgtac gtccgccgcc
     8761 gtcaaagcct ccccagaaag agttgctata tgtacctatc ggcgtccact ccttaacgct
     8821 aaggtcaagg tcagaaacca gcagaaaatg cttccctcga taattcgtgc cattattcac
     8881 ctgctgcgct agatacgcaa gctgctctgc attttcaatc cgccagggat cttcctttgt
     8941 gccactacct tcggtaaaag cttccactgt gccatcccag gtatctactg tttccccttc
     9001 cgctcttgcg ctcaccggca cggcgatcca caccagtgcc gccagggcca gcagggccag
     9061 tctcatcttc atgctccatg cttttgtact tttcttcata accctcatgc ctttctatat
     9121 cctgcaaaat ttcaaatatt tcccaaccct ttgcagatgt aaaatccaat tttgttttta
     9181 tactacccgt atcatttatt ataccgaaaa atcaatagta acgtcaatat tttccatacg
     9241 attcgtctca tatttttata aaattttgtc aaacaagtgc gcccaccgca aaatcgagca
     9301 ggctggctca tcgtaagcac aaaaaaggag ccaccggatc tctccggtca ctcctcgttg
     9361 ccttctctga aaaatatatc cagttttccc agacatttga tcaatgtctt catttcttcc
     9421 tcgtccagat cccgtatgat ggtgtggatc attttcctgt ggaaatctct gtgatggaaa
     9481 aaagcttttc tccccttctc tgtcagggtc accagcacta cacgtttatc tctggtgctg
     9541 cggttgcggg tgagatatcc ttttctctcc agactgttca tattcgtggt cagggttccc
     9601 acggtgacat gcagttcccc ggcgatcctg gacatgctct tgggttcctc gatgccgata
     9661 gcctccagaa tgtgtatgtc attatttgta atatccttga attcttctgt tatgaccgcc
     9721 tttgcctctg catccatcac gttatggaac aggttcacca acagttcatt cagtacccgg
     9781 ttgttgctct tctccatagt tttcccaata aatgtctctc tggtataccg gcaatactcc
     9841 cgggccgcag gcttcctgtc taccggaccg cgtccatgcc gttgtcgtgt ccttagtcgt
     9901 tatcagtata ccgtctacca ggtcatcacg caagccccaa tcgtaagccc ggcaccgaat
     9961 cccgccagaa cgatgcggtc gccgcggtgc agctttccct gcctgtgcag ctcatccagc
    10021 agtaccggga tagaagcgga ggacagattt cctaccctct ccagatttgt aggaaatttt
    10081 tcacgatccg catgaaggcg ttttgccact gcatcaatga tccgtgcgtt ggcctgatgc
    10141 agtacgaaca ggtcaatatc cggcacagca agtccggcat ctgacagtgc ttcctcgatg
    10201 cactgcggca cccgtctggt agcgaaccgg tacacttcct gtccgtccat ctgaatataa
    10261 ggttctctgc tgtccgtcgt ctggttttgg tccgtttttg ccgccgaagc ccttgcatac
    10321 ggggttgtca attctctcgc tccacattgc aggactccac cgccggtgcc gtcggagtgc
    10381 agtgcccggc ccagaatgcc tgctggcggt atccgtttct cctccgccgt ctcctgcatc
    10441 cctttccctg tctccggcaa agcgttactg tattccaccg cccgagactc cgtcctgcat
    10501 ctctccacaa ctaccgcccc ggcgccgtct ccgaacagaa tacagctgcc ccggtccgtc
    10561 cagtccacca gcttggacag tacctcactg ccgatgatca gggcattttt cgccaatcct
    10621 gtcctcagat aagcatctgc ggtattcagt gcaaacaaaa atccggaaca ggccgcattc
    10681 acatcaaaag cca
//
LOCUS       DAWDHD010000080        10657 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_384_length_10657_cov_8.775231, whole genome shotgun sequence.
ACCESSION   DAWDHD010000080 DAWDHD010000000
VERSION     DAWDHD010000080.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 10657)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10657)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10657
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_384_length_10657_cov_8.775231"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2850..2949
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2404 a         3093 c         1997 g         3063 t
ORIGIN      
        1 tcgtagcttt caagggtatg actgtagaag ctgcaggtca ggatgctgac ggcaacgatg
       61 ttgctttcct gtacaactgg gatggttccg gtgaagacgg taacgacctg tacttcaacg
      121 tatccctgaa cggtcagacc tataccttca ccgttgagtc ttatctgtgc gacaacacca
      181 ccgatgttta caacgcagta aagaacttaa agatcggtga caccatcgat atggaaggtt
      241 tcctgtactg gtacgaaggt gtgaaccctc acatcacttc cgtgactgtg aagtaatttt
      301 atcagatatt aagctatatt gacaataaaa agaactggct tcccaggaag acagttcttt
      361 tttattgtcc acttatatgc tttacttatc tgttgccagg tacacctgcg catacctatc
      421 tttctctctg cctgtcggaa ttcttctgcc tccatcagcg tctcagcaat ctccttctct
      481 ggctctacta tctatgcgaa gtgatcaacc tttattttct gctctccagc cattgtttcg
      541 cgatttggaa tccctccatg ggaacgcaga atcgctgatc tctaggcggc tgcaatgcgg
      601 catccagttc ctccatatcg aaccactcca cactctcaac ttcctctttt tgcaggataa
      661 gcttcgcaac atctaccggc ttagtgtaca tatagacgaa tgcaacctcg ttgtcccgaa
      721 acatttttcc atgaaattcc ttttcgtatt gaatatcaaa gcatccggcg aattgcagtt
      781 cctctttaga tgcctggatt cccagttctt cgtaaagttc ccgtattgca gattcctccg
      841 gctcgtcccc cgcctgaata tgtccggcgg aagaagtatc gaacctcccc ggaaaactgt
      901 ctttctcagc ggagcgtttc tgcaataaca gctgggcttt tccgttctgt tccctcacga
      961 tccagatatg ggcggttctg tggcggacac cctttttatg tgcctgtgtg cgttctatgc
     1021 tctcccctgt gggaatgcct ttttcatcta cgatgtcaaa tatttccata gtgttatggc
     1081 tcctttgtgt ttctttgtag ttttcctatc taagatctaa taccatcaga tcaaaatcgt
     1141 agtattcccc gtcgatctta aaatacgccg gcgaggtccc gattttccgg aagccatatt
     1201 tctcatagac atgaatagct cgtatattat cgcttcgaac ttccagattg atcagttcta
     1261 tcgtatgttc ttttgcataa tcaataatct tttgcaatag agcactgccg atgccctgtc
     1321 cccattcgga ctttgttaca gaaatgccga attcggcgcg gtggctcatt cttctgggaa
     1381 acccgctcaa gctggcatct ccgacgatct ctccattttt ccatactaca tagagaacag
     1441 aatgttcctc cttatttata ttctgtatga atttctcttc ctcttctgtc gaaataggaa
     1501 gcccttcttt tccaaaggac agataatctg tctctccacc tacctgcgct aaatatgcga
     1561 tgatcttctc cgcatcctcc ggtgtagctt ctctgatatc aaattcttcc atagctgcct
     1621 ccccttcgca agattacaaa ttttattcat aatcaaagat agtcttggac tgaatagcat
     1681 ctccgtattt tgtcttccat tcctgatggg tcgcagaagc atcgtacaca ggcagtgctt
     1741 ccggatccat ctccatttcg atcatcaggt catgacggtt cggtctcggc gtcacatttt
     1801 cataaatgtt caccgtatga acgccttcta tctgcttcgt ccgattgaag atcttcttga
     1861 tgtccgcaag catctccctg ctcgccgcat cctcccggtt ccatatattc tctttaaatt
     1921 ttacaatgat caggtgcttc attttgcata tcccccttat tttactatta ctgatatgtt
     1981 ttatcctacc acggcatccc atttgctaca accaaaatat tcctgcaaga tcatttctac
     2041 tttgtctgca ttcagaggac actgctttgt ttcataggtg agaatcagat ttaccgaagg
     2101 aatgatacca tgtcggcaat atagctttat tttgtcacaa gcatgattgc aataatccgg
     2161 attatccatc atcccaaagt gctcccaata gaaatattgt cccgtaaagg gatgtcgcat
     2221 cacgaagtcc ggatataaaa ttattccatc caaaatcagt ttttcttcat agtggaatgg
     2281 tattttgttt tgatatagtg ttctgtcaat aattgcttct gatttagacc gcaacatctt
     2341 cccatgcgtt ccttgtacca ataaattttc cggatgcttt gtacattttt catagggttc
     2401 ctcctgccac cgctccagtt ccttatccaa tggtctgaaa ttcttagcca gcaattttcc
     2461 atattccgga tgatttaata gatgttcgga gtttattttc atcttgtctg tcttcttcaa
     2521 gtaggcttcc catcccgctg cttcactctc caattccttt tttctatact catagtattt
     2581 cttcaatgcc agtatttctg cctgatttct ttcggttttc ggaagatagc gtattccatt
     2641 ttcctctttt actttccatt tgtagcggtt ttcatttttg aagcattgca attctccttg
     2701 cggaaatttt ttgtccaatt ttgttatctt ttctaattcc ttattaattt cttctattct
     2761 gttctttata ttttcgcgtg tatttttcac ttttcctcct tctgcacact ggcttcttga
     2821 cttctccggg gcttattctt acttttcttn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnc cggggcttat tcttactttt cttcctctca cgccccggct tcttgacttc
     3001 cccggggctt attcttactt ttcttcctct cacgccccgg ctacctagaa ccccaacggc
     3061 ttattttctt ctcagatacc cttgcagccg gtttccgtga ctgtcgacgg ttactgtcag
     3121 ggctccatag tcagtggtga ggtaccaggg aaccttagca tcttccaggc gctgtagcgt
     3181 cgcggcatgg ggatggccat aggaattgtt acgaccacag gagataatgg cggcagcggg
     3241 gccggcggcg gcaaggaact cagaactggt ggaatagcct gagccgtgat gcgctacctt
     3301 caggatgtct accttgaagc caccttgtct ccgtgattcc cacaactcct gctgttctcg
     3361 cggttcctgc aatttccgcg cattctgcaa agcccgtgat tcctgcgcta cccgtaacgt
     3421 cttcgcttcc tgtaacgtct tcaacgtctg caattcctgt gtcagctgct gctccccttc
     3481 gccttccaca tcccctgtca ggagaatgct tagcttcccg gcacccacgc caaagttttt
     3541 cctttcaccg gtatcaccat gaccggttct ctcaccaata actccgacag caaagctgcc
     3601 gttctcaccg taaacttcgc tagcagctct tccgccctct ccacttgctt ccatcccctc
     3661 tgccgcgccc cacttcactg ctttcgcaaa tacctccaca tagaagcatt ccgaataggc
     3721 attggcatcc gcaagagtcg tattctcctc cgggtgcaga caccgcagcc ggaggagact
     3781 atccctgatc tcatcgccac attttatgta acttaccgat actccggcat attccgcggc
     3841 aacaagcaat ttttcaaaag cctccctgcg ctccgcttcc gtaatcgcag gcagaaacat
     3901 ctgctccacg gtaattcccc attctccacc gttctccagc agttccaata tgccattcat
     3961 atggtcttca tctgggtgcg atacaaacac accgcgtaga gactggattc cccggctctt
     4021 gagataaggc ttcaacacat attcaccgat tttcctccga ctggtactgc cgcagtcaaa
     4081 aagatatgcc ccctgcccgg tctccaccac gataccgtct ccctgtccca cgtccagaaa
     4141 agtcacacgg cttccccgat caaaattgcc ggtcagtaaa ccaactatta atactaaaat
     4201 cattgcggct acaattcggc acataactcc gttcctatat gtcatcactc cctgccatgt
     4261 ataccaaaaa tttgctaaaa catgccgcca tctggtggga catatttcag agtacctgtc
     4321 agaatgctgc atcttttttc tgttactctc tctttttcta cccctaccgc tatcatccac
     4381 tccgcgtgtt tcccgacgct gcctttctct ttcagcgcag tgtccgtcgc catgattatt
     4441 ttccgccaca taagctgcat aaagtctttg ccgcttccat ttttccgcat aattcctgcc
     4501 aatgatcaac acaaatagcc cactataata aacggccatc tgccactttg ccgggcggcc
     4561 cacgcaccat accgctcccg gcagttctcc gaaccgttcg cagatccatt cataccacca
     4621 caggatcaga caatccaccg tccctgcaat tcccagtccc gggatcagtg acaggattcc
     4681 ccctgccatc accacactca taaagggcaa caccagcagg ttcaataata ccgagtagac
     4741 agggatctca taaaaaaacc agagctgtat gggcagcgta aaaagaagaa ctccggcact
     4801 ggcggcaaaa ccgcttttaa gtgctgacac catgcgccaa agcctttcat acaccacctt
     4861 ccgccagcct tcccggtact tgttcctctc gtaagcggaa tcacccagaa tatctgtcac
     4921 ggtcagaagc catgccggta ctctaaaacc gttccccccg tttcccagtc tcctctgttc
     4981 cgtgtcaaag tcagacctct caccaaatcc gctcttccca tcagtcctct ttttctctgc
     5041 aaagcctaat gcctcaccaa gtccgcgctt cccgtcggcc ctctttttct cttcaagccc
     5101 tgccaccaca ccactggccg tttccacacc gacattatct tccctaccat ccccggcaaa
     5161 caccggtgtc aacaagcaga tccccagcac cgccccaaaa gacatcaaaa agctcacatc
     5221 cccaagccgc tccggttgct gcaacaccag caatgccgcc aacagcccca cacctgtcag
     5281 catatcgtaa gtccttccca cgaatattcc caacatctgc agcagataca ttcctatggc
     5341 acggctggcg gatacactca tgcccaccat cataccgtac agaagcagtg tcatggcacc
     5401 tcccgctgct gccactctga ccggagcccc cagtctctta agcagccggt agcatcccat
     5461 ccccagcaaa gtaatatgca gccctgaaat gcttaagata tgggcgatcc cattcctctg
     5521 gtacagcgcc tttacctccg catccagttc ttccttctcc cccagcagta atgtctgcat
     5581 aacggaagcc tctttctccg ggaatacctt tgccagtctg tcgtgtaatc tccttcgaaa
     5641 ggcaaacaac ttttctctca aaaagctata gtcctcctcc gctgccagaa cctgtgcttt
     5701 ccgcactctg ccaccgatgc cctttcccgc agaataggcc cgcacgtcaa attccccgtg
     5761 attggtcgca gcatcgaagt gcgaaaaaac gccttccaga acaatatgct ggcccagtct
     5821 caggctctgt acctcctgtg tgtttgtcaa ctcacattgt aattttccag aataggaata
     5881 ttttagatcg ttttgaataa tagaaattga tttcagaata atcgtttcag aagttcttgt
     5941 gtctatccgt cccgtgatcc tcaccggact tccgtcaggc ggcagtacgc cggcatcccc
     6001 ggtgtctgcc cccgtcagta ttcttcctat gacgagcact atcacgagac acagacacgc
     6061 cataaaaagc ggtcgtctca ttaagctccc ttccgcaggc aatcccgcct gcccacaggc
     6121 aggactgttc cgacagtcca acgcaaaaac agtcctgtcc cgcataaatt caaaatttac
     6181 tgttccaaag tcgttacata atccagattc cgttcaaaag tggaattgct gaaggtagat
     6241 ggcacttcgc cgttgaccag gatctgtact ttattgatat tatccagttc caccagggaa
     6301 ttcacgatgg agtaaactgc tacctccgtg gaaacattgt tcaccacggt caggaagttg
     6361 gaatccagat tcacatagca gatgccatct ttggtcagga tattgatgat cttggtctcc
     6421 ggattgatca ccgggtacag accctcaatc tgtccgctgg gtcccgcgat tagttcctcc
     6481 accacgaatc gctccaacgg agtattggtg gaataatgct tttctctgta ggccgcgatc
     6541 agcttatcac cgtctgcatt ggcaaaatac agctttacct tcaccagttc ataggtgttg
     6601 atctcgttac cgtcattatt gatgaactgc tcggcattca tccagcctac caattcccct
     6661 gcattgtcat ataactgact gccttccacg gtaatcgtca catatttcac attggacagc
     6721 tgcgtcagtg tccttacaac ggccgcccgc accagtactt ctgtggtgac cgaaagttcg
     6781 gaatatgcct tatccacatt gattacaagt tttccgtctt ccacttcata atccagaacc
     6841 tgaaatccca tgtcaaaagg cgccttatat tccagctttt ccggggtggt ggagagaaat
     6901 gtcatggtct cttccaactg ttcctccggt gtcttggtgt tcagatactg gtcatgaacc
     6961 tcaaccttcg tctctgcggt atttacataa tacaccgata tctttttgcc ggtctccggc
     7021 tctctcgtgc cacagccggt caggactgcc agcaacatca gaagcatacc ggtcaaaaca
     7081 tattttttaa ctctcttcat aactacacaa tctttcttac aacagctccg tctaagaggc
     7141 gatatatgtc agcggaatct tcaccaggaa acaggagccc tgcccttcgg tactcgtcac
     7201 cgtaagggat cccttgtgca tcaggacggc gctcttggtg atcgccagtc ccagtcccgt
     7261 tccgccgatc tctctggaat gagacttgtc cacacgatag aatctctcgc agatatgtgc
     7321 cagagcctcc tccgggatac cgattccgga atcactgact tcaaaggtaa aatactgatg
     7381 atccgcatcc agtatcacct tcacccagcc atgatcctta ttgtatttga tggcattctc
     7441 caccagattc gtcatgatca gggtcatctt gacttcatcc acctctgcca ccacaggacg
     7501 gacgctttca aataccactt ccacatcctt cttccgggcg atgggccgca gtctctttaa
     7561 gatcagttcc gtcagatcat tgatgttcaa cgatacaata ttcagatcct gtaccttttt
     7621 atccattttt accagtgcca gcagatccgt aatgatctgg ttctctctgt caatctcgga
     7681 agcaatgtct tccatgaatt ctcgatacag ctccgccggt acattttcct gtgctagcag
     7741 ggaatccgac aataccttga tggatgtcat gggtgttttc aactcgtggg acacatttgc
     7801 cacaaattcc tgtcgggaat cgtccaatgc cttcatccgc ttcaacaact ggttgaacgc
     7861 atctacgata tgtacggtct ccacataatc tggcacagag atcgtctcat cactgaagcc
     7921 ttcctttacc gcattgatgg cctgtgtgac tctgttgaaa ggtctcgtca aagctctgga
     7981 cagtatgata gccagtgcca ggattgccac catcatgatc acttccagaa tgtacgcctt
     8041 acgattcagc acttccttgg catttacgat actgtcattg gaaatgctgg tcagcataac
     8101 gcccctgatg gcatccggat tcctttttcc gttttcgtcc gtagtgtttg ccgtggtatc
     8161 catgatggga gtggtcattt ctatgtaacc gtgctccggg tcataatggg aaatgttttc
     8221 cccctgaaaa catttgatga cctcctcgga aatgatggtc tttccttcac tgatgccgta
     8281 ggtatctttt tccaccttga aattcttgtt gatgatcata acacggcctt catacagatt
     8341 ggaaagcatc tccagttccg cattgatgac ttctctggaa ttgccggtgt cctctctgac
     8401 accatggctg cttaaataat tgttgctgat cagatgattg gccaggatca tcaactggtt
     8461 ctgtaccact gtggtccgct gttccaccgc cagatcctcg taattgctca cgataccgtg
     8521 gcgcatggca atacaaggca ccaggccgat caccagcgtc agcaggaaga tcctcgcccg
     8581 cagacttcga agcgcgatca tgctctccag cttttcccat atagttgcaa aacgttcttt
     8641 attccatttt ttcacagtat aaaaacctca gttccgaacc tctccacgcc attcacaaaa
     8701 ccgcacgata aatcgtgctc tccgctgctt cgcagctact tttgttcatt tgcgtggcgg
     8761 tggctctatt cgagccacct tcaaagataa gagaaattga ttcttacatg caagcatggc
     8821 gaatcatttc ttcttatctt tgaaggttct catagtctca cttattatta aagtagtacc
     8881 cgacgcccca tttggtatgc acgtatctgg gctcgctggg attggactcg atcttctcac
     8941 gcagtctccg gatatgtaca tccactgtac gcacatcacc gggataatcc gtaccccata
     9001 ccagcttcaa caaattctcc ctgctgtata ccttgtcggg attcagcatc agaagttcca
     9061 gcacttcgaa ctccttggcc gtcagtccga tctccctgcc ctggatgtag gctcttttgc
     9121 cgtcacgatc cagcttcata tcgccgcttt ccagaatacc ggtgttccct gtggttcctt
     9181 ttttcggctc cattctgcgg aagatagctt tgatccgggc tttcacttcc agaatgttaa
     9241 aaggcttggt tatgtaatcg tccgcgccgt attccagtcc caggatttta tccatgtcat
     9301 cacccttggc ggtcaacatg atcaccggta cattggaaaa ttcgcggatc tgctggcaga
     9361 cctcaaagcc ggtcagcttt ggcagcatga cgtccagtaa tatgatgtca tattccttgt
     9421 tatgcgccat ctgcaatgct tcctcaccgt cataagcaca atccacttcc atgttgtcct
     9481 gttccagact gaaacggatt cccttcacga tcagcttctc atcatccact accagcactt
     9541 tcttgcctgc catctttcct taacctctct atcccttact tttctcttca cggtcccggc
     9601 ataagccctc attgtaccgt gatcttatca cacaatttgt catacgcgtt ctgtttgatc
     9661 ccggatacct tcataatgtc ttctttcgtc tgaaaggctc cgttttgctc ccggtagcgc
     9721 acaatatctg ccgcccggga agctccgatc cccggcagcg cacacaattc ctccgcaggt
     9781 gccgtgttga tattcacaag tccctcctcc gtcgctttta cctcggcttc cagagtctcc
     9841 gcttcctcca acgttggaaa atacagcttc tgaccatccg tcaccgtttc tgccagattc
     9901 acataaaaag gatccgcctc cgctgtcata cctccgacca gtcgcagggc ttcctccgct
     9961 ctgctgccct ccggcacttc cacgactccg ggatcccgca ctgcgccgca gacatagaca
    10021 tatatcatct gcggttcagc ctttgccggt tcctgcactg tcacgctgct gcctgcaggc
    10081 tccgctgcct gttccaccgc ttccagtgag ggaaattccg ccacatcttc tccggatgga
    10141 atcagcacaa tggcctcatc cttcctctga cagccggtca taaacatcac cagactgaaa
    10201 aacattccga tcaaacctgc atattttgtt ttaaaaaacc ttatcgaaaa catcatagtc
    10261 ccctttccat tttcctgatt ccttcacaaa atttcagggg attgcctctc tatgctgtta
    10321 actttcattg tactggaatc gcacggaatt tacaagccgt tttcgcattt gtggtaacga
    10381 ttcagaaccc attcgcaaaa ttgccgctac gaggatagct ctggatcatt tccacttgaa
    10441 aatcacaata cccacaatcg ccacggccat gccgatggct ttccgccact cccagggctg
    10501 cttctccaca ccgaaccatc cgaacagttc gataatatat gctacagcaa gctgagcgac
    10561 cacaataagc attaccgctt aggccggtcc cagggcatcc attctgcgga tcacagtgta
    10621 ggtgataaag gctccgatag cccctcccaa aagcata
//
LOCUS       DAWDHD010000081        10351 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_388_length_10351_cov_8.668318, whole genome shotgun sequence.
ACCESSION   DAWDHD010000081 DAWDHD010000000
VERSION     DAWDHD010000081.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 10351)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10351)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10351
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_388_length_10351_cov_8.668318"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2190 a         3020 c         2120 g         3021 t
ORIGIN      
        1 atgatctgta aaaattcctc tacgcttttg gcatttctta aagatgtggt aaaattctca
       61 tccatcagca taacggacaa tttgctcagt acatccagat gtacattatc cttggtattg
      121 ggtgctgcga tcaggaaaag cagtgtcacc ggttcgccgt ccagggaatc ataatccaca
      181 ccgtttttaa ttaccatggc tgccagtccg ggagtcttca ctgcatcaca tttgccgtga
      241 gggattgcaa tgccttcgcc gattcccgtg gtgctctctt cctctcttgc atagacctgt
      301 gcacggtact gctccacatc cataagcttc ccgctcttcg ccatcagttc cactgccata
      361 tccagtgttg cctttttatc aataggagtt gcatccaggg aaatacttct tttgtccaat
      421 aaatccgtaa ttctcatatt gtaacccttc ctctcttttt tattgtttct tttttattat
      481 gatgtcgagg tcaaaaacct ctactttttt acctgtttca atacctgcat aacttcatct
      541 ttggtagcca gattctcaga aaaagcgctg gcgcttcccg cggcgattcc catgcagaag
      601 gcatgtgcgt attccttctc ttccagccat ccggtgagga atcccgctac catggaatct
      661 ccggcaccga caccgttgac cagctttccc ttcggagccg gagcctccag gcattgaccg
      721 tccgctgcga tcagcaccgc tccttctccc gccatggaga tcaaaacatt ctgtgctccc
      781 atgtcccgga gctttttccc ataagggacc acttcctctc tggtggaaag cttcacgccg
      841 aagatatccc ccagttcatg gttgttgggt tttaccagaa aaggatgata cggcaatacc
      901 tttaccagca ggtctctggt ggcatccact acggtgagaa cacctcttcc ctgaattctc
      961 tccagaattc tctgataaat atcatccggc atcgaatagg gaatacttcc cgccagtacc
     1021 aggacatctc cttcctgtaa tctgtcaatt ttttccatca gctgttccag cgcttccgca
     1081 tcgatatccg gtccgcagcc attgatctct gtaccgtcaa tacttttcag cttcagattg
     1141 attctggtca ttccgtgatt gattttgatg aaatcggcat ttactccaaa ttcagtcaat
     1201 cttcggatca attcttctcc ggtgaatccc gccgcatatc ccagcgctgt ggaatccagg
     1261 cccagattct tcagcacaat ggacacgttg attccctttc ctcccggaag aatcagctct
     1321 gatgaagttc tgttggtcat tcccagctga aagtcatcca cggatacgat gtaatccagt
     1381 gacggattga atgtcacagt acaaatcatt tccattcctc ctgtctgtta cctttcgtgt
     1441 tcttatgatc ttattatatt ctcacaatcg gtcaaattca atcaatttaa ttcacaaatt
     1501 tggtgattta ttttggttga atttgcacaa aataaaagga tgaagtattt cttcatcctt
     1561 tttcaatgtt tctttcacta tagcatttcc cttatctggc ttctttaaac ccgtttgcct
     1621 gttctttcat ttcctttgca ttggaaagcg ccgcttccgt cagtgcatca ctgcctaaca
     1681 gtcttgccag ctccgccagg ctttcctctt ctgccacctt gtgaatgtcc gtaatggtca
     1741 tatcatcggt actgctcttt tcaataatga aatgcacatc cgccatggct gcgatctgag
     1801 gcagatgagt gatacagatg acctgatggg ctcctgccag aatactcagc tgctccgcta
     1861 ctttccatgc ggttctgccg ctgataccgg tatcgatctc atcaaagatc aatgtatcaa
     1921 tggcatcttt ccctgccagt accgttttga tcgccagcat cacccgggac agttcaccgc
     1981 cactggccac ctggttcaaa ggtttcagac tttctccggg attggtggaa atcaggaatt
     2041 ccacatcatc atagcctttt gctgtaatgg cctgaccggg tctgactgcg atgtcaaatt
     2101 ccaccgtcag gaaattgaga cctatcagcg catctttcaa tttctgcgtc agtaccgctg
     2161 cattttttct gcggatctca gacagttctt cacagtcttt cttcagtaac gcctgttttg
     2221 cctcccacac gacgttcaat ttctgcatat aggcatcata atccgccagt ttttccagat
     2281 aagcttcttt ttcctctttg taccgcagga tctcttcgat cgtgttgccg tattttccct
     2341 tgaggtgatt gatggtattc aggcgatcct ctgtactgcg aaaatcctct tcatcaaatt
     2401 ccagatccga catatattcg gagacatcat gattgtaatc tgacaaaaga ctttccacct
     2461 ctgccagctg ttcctccatc tgttccagtg caggatcata catggtcaca ctacgcaatg
     2521 cccgcagtgc cctgctcaaa gaattcccgg caccgtcttc cgcgtcattg ccactgagtt
     2581 gatagctctc cgccaggctt tccgcaattt ttcggctgtt ggacattttg cggtacgcct
     2641 gttccagttc ctcatcttct ccgggctgca gggaagcatc cgcgatctcc ttttgttcaa
     2701 attccgccag agattgttct ctgcttctgg aggtatcatc cagagtttcc tctgtgatct
     2761 ttttctccag ggcagtacac tcatgataaa gtgcagccac ccgccctgcg cattctgcaa
     2821 attccgctcc cgcataggca tccaggatct ccatatgttt tttcttgttt agcagagact
     2881 ggtgctcatg ttgtccgtga atatcgatca gcagctccga aagttccttc acctgctttg
     2941 ccgtcacggt ctctccattg atgcggcaga tgcttttccc ggaggaaagt tttcggctca
     3001 ggatcacggt tccgtcttcc gaaggttcca gatccatgct ctttaatttt tccgccagcc
     3061 tttcttcctc gcagtcgaag accagttcca ccagaccgtt ctcttctccc cgtctgagca
     3121 tgtctttctc gaactttcct cccagtgcca gattcactga tccaatgagt aatgatttac
     3181 cggcaccggt ctcaccggtc agtatattca gtccttctcc gaactctacc tccgtctccc
     3241 ggataagagc cagatttttc acgcgtaaac tacgaagcat gtatttcccc ctgtcttacc
     3301 tttctatcat cagatggatc ttgtccataa ggatctgggt atcttccacg gtccgcactg
     3361 ccaccatgat cgtatcatct ccggcgatac atcccaccac ttcggagaat ttcagggcat
     3421 ccagtgccgc cgccactgcc atggccatgc cggatacggt cttgatcacc agaatattct
     3481 gtgccatatc catggagata aaaccgtctc tgaggacgcg gatgtattta tctcccatat
     3541 gactgtcatc ctgttttaaa atgacgtatt tctgtttgcc gccaccggtg ggtactttgg
     3601 acagcttcag ttctctgata tctctggata cggtagcctg tgtcacagca aaaccggctt
     3661 ccttcagata gcccgccagt tcctcctggg tctccacatc tcttttttct ataatctcca
     3721 cgatcttgcg atgtcttgtt ttcttcatct tgacctcatg cctttctcac aacaattcgt
     3781 caggaatctc tcattttccg atgcaataca tccaggaagc ttacctgatt cagctgaata
     3841 aactcggtga tcttctccga ctgcagaata cggttcaggt ctgcggtaca catggtcact
     3901 ttgtggctgc cgtcataatt ggcttccacg gtctggcttt tgccctctct tccaccggga
     3961 atctctataa cgatctcatc ttccggggac aggataatgc ttctctggtt aaggctgtgg
     4021 gggcagatcg gtgtcagggt gatcagtttc gcctttggct ccaccagcgg cccgcccgcc
     4081 gacagattat atcccgtgga tcctgtcggt gtagtcacga tcattccatc tgccttgtaa
     4141 tgattcagaa gctgtccgtt gacatagatg ttgaattcta tgatctgcag ggatccgctt
     4201 cgggaaatga cgatgtcatt caaagcccag ccttccgaca cggtttcatc cgcacagcag
     4261 actttaccgt ttaacatcat tctgcgttct ttcttaaaat catcccggat caggcggtcc
     4321 agggattcct ctaatccaga cagttcgatc tccgtcatat aacctacggt tcccagattc
     4381 actccgatga tggggacaag gatcttcttc gtctctctgg ctgcctgcag caccgtgccg
     4441 tcgccgccca atacgatcat gcagtccaca tctaacggaa tgtccgttgc ttcctcttcc
     4501 gccacggaat ccgcttcttc cttccagtcg gattccttga cctttaattt caccgtacag
     4561 cctttggttt ccagataatt ctggatccgt ttcgtggtcg caagcttctg atccttatgc
     4621 ttgttggtat atatcagaaa atgcttcata gcttcgcact ccttgtctgt tacagggtat
     4681 gagaattgtc tacgatacct ttgattacgg catcccattc aggggtatag gacagtccgg
     4741 tcttttcctc tataatagtc tgtaaggcgc tctccgcttc atgctcggtc agcgccagct
     4801 tttcttcgga gatctcctcc ggcttacgga tatgcagcag atattcgata ttgccctccg
     4861 gtcccttgat gggggaatat tccagatgca gtacttcgaa accgatgctg tccgcatagt
     4921 cgatgatctt gcggatcact tcctcgtgga tctgaggctc tctcaccaca ccgttcttgc
     4981 ccaccttgcc tctgccggcc tcgaactggg gcttgatcag acacaccatc tgtccgccgg
     5041 tctttaacag atttctcgcc gggatcaaaa tcttggtcag agaaataaag gacacatcca
     5101 cgctggcgaa atccagtgca tccgggatat cctccggggt cacataacgg aaattggtct
     5161 gttccatgca taccacccgc tcatcattcc gcagtttcca ggctaactgc ccctgtccca
     5221 cgtccacgga atacaccttc gtggctccgt tctggagcat gcagtccgtg aagcctccgg
     5281 tagatgcacc gatatccatg cagatcatgc cgtccagcag aaatccccac tgttccattg
     5341 ccttttccag cttcaggcct ccgcggctga catatttcat ggtatgattt ctgacttcca
     5401 gcttgatctt gctgacatca aatccggtgc cggccttgtc ttccttctgt ccgttgacat
     5461 agacattgcc ggacattatg atggcttttg ccttttctct ggaaggagca tacccctgct
     5521 ctaccagaat cacatccagt cgttctttca tcttcgtctc ctaattattt ataatctttc
     5581 cagaattttc tccgtgatgc tttccgcatc caatccgatc tcctttttca aaagctctac
     5641 atttccatgt tccacataag catcgggaat agctgccatg acacatttga tccgtttctc
     5701 gtggtcattc agacaatcca acaccttttc tccgaatcct ccgctttgga cattttcttc
     5761 catggtcacg atcatctcat gatcttcaca ggccgaaagt actgcctctt cgtcaatggg
     5821 cttgataaat ctggcattga tgatacttgc cttgtaaccc ttttcatgca gaagcttctg
     5881 gacttcctcc gctgtcttta ccatactgcc tacggcaaac aatgcgatac cgccctctct
     5941 tgcgatccac tccgccttgc ccagttcaat gggggcacgg tgttcggcaa gtccggcata
     6001 agccgttcct ctgggatatc gcaccgcaat gggcgcatcc agtgccacgg caaacttcat
     6061 catatccgat aattcccatt tgttcttcgg cgcacagata tgcatattgg gaattcccga
     6121 cagataggta taatcaaaaa taccctgatg ggtctcaccg tcactcccca ccagccctgc
     6181 acgatctata gcaaacacta ccggcagatt ctgaatacac acatcatgca ggatctgatc
     6241 gtaggctctc tgtaaaaatg aagaatacac cgcaacaata ggcttcatgc ctcctgctgc
     6301 cagtcctgct gcgaaggtca ccgcatgttc ctccgcaatt cccacatcaa agaaccggtc
     6361 cgggtacata ttccggaagc gctttaagcc ggtgccgtcc ggcatggctg ccgtaattgc
     6421 cactacccgt tcgtcccgct ggcccaactt gcacataaca gtggaaaaca catccgtata
     6481 attggccacg gtgcggggat gggaaggaat tcccgtctcg atgtcaaaag gctctgcccc
     6541 gtggaatctg gcgggatgtt tctccgccgg tccatagccc ttgcccttct gggtaatcac
     6601 atgcaccagc acagctccct tgactctctt ggcttcctca atgaccttga tcaggccttc
     6661 gatatcatgt ccgtctaccg ggcctaagta agtcactccc atatcctcaa aaaacatccc
     6721 ggggatcacc agctgtttga agctgctctt ggcccggcgg atactgttga caataccgtc
     6781 gccaccgggt tttctcttga gagcgttgta tatgccctct ttcagatcca gatagccggt
     6841 cgccgtacgg atattgttca gatatttgga aacaccgccc acattttcgg agatggacat
     6901 attgttgtcg tttaatacca caataaagtt cgtctccagt ttggctgcat tgttcaaggc
     6961 ctcgtaagcc atgcctcctg tcagagagcc gtctccgatc acggatacaa tggtggtctt
     7021 ctcccctttg agatctcttg ccttcaccag tcccagtcct gcggatatgg aggtagaact
     7081 gtgtccggta tcaaatgcat cataatcact ttcttttctc ttggggaacc cactcatgcc
     7141 atggaactgg cgtaagacat cgaaaccgtc cttgcggccc gttaatattt tgtgggtata
     7201 agactgatga cctacatccc agatgatctt gtcctccggg agattcagag caagatgaag
     7261 tgccatggtc agttccaccg ctcccaggtt cgaacccaga tgccccccgg tcacactgat
     7321 cttttcgatc aggaactgcc gaatctcctc cgccagttcc ccatagtctg atttatctat
     7381 ttttttgata tcgtttgctt tctcgatctt atccagtaac attggctttt cctctgctat
     7441 ttcgttctat ggaccaggtg cagcaacagc tccttcagga attcatgttc tcccgaaaag
     7501 ctgtccagaa tcgctaccgc ttccccggtc agccgttcca cttctctgcc ggattcttcc
     7561 agtccgtgta aagtcacata ggtctgctta tgattctgtg catcacttcc ggtagctttt
     7621 cccagctcct tgctgtcccc tatcacatcc agaatgtcat cctggatctg gaatgcaatg
     7681 ccgacattgt aggcacacag ttccaatgcc gcaatctctt catcggttgc ccctgccagc
     7741 gtagctccga tagtcatggc cgactggatc agggctgccg tcttgttggc atggatgaac
     7801 aggatctgtt ctgtggtcag aggttctgcc tgttcctccg cttcaatatc tgcgttctgg
     7861 ccgccgatca tcccgtagat tcctgccttg cgggctaaaa tattcagtgc cgccgccact
     7921 ctcgggtaat tcggatctac cggttctccc gaggtgcatg ccggaaaagc acgaaatgca
     7981 gtctcaaagg cataattgag cagtccgtct cctgccagga ctcccatacc gtcaccgtac
     8041 acagaccagg tggtcttgcg gcctctgcgg tactcgtcat tgtccatggc cggcagatca
     8101 tcatgtacca gagaatatgt atggatcatc tcgatcgccg ccataaaagg ctctatcgta
     8161 tcatctttgc caccgaacat tttataggtc tcctgcatca gcatgggacg cagacgcttt
     8221 ccgcctgcca taaggctgta attcatagct tcaataacgg tcttctggta tccttcctcc
     8281 gccggaagat attccttaag caattcttcg attgctgccg ttctcttctg caattcctgt
     8341 ctgaataatg tttcttccat gtctgatgcc aaccttcctc tacctgccgg tatacttgta
     8401 tgcttactgg ggatccagcg gttccagtcc gccctcttcg ttcagcttca tgacctgttt
     8461 ttccacccgg tcgatctgtt cgctgcactg cttcagcaga ctcatgccct tactgtatgc
     8521 ggtaaaagcc tcctccaggg aaatatcctc tgcttccagc tgtccgatca gttcttccag
     8581 tgcggaaaaa ttctcttcca ggctctgtgg ttcctgtgct tccgttttct tttttcctgc
     8641 caaaatagca cctccgtctg ctgcccgcat ctatctgcat ccccccggat accggtaaat
     8701 gctatccggg aagtctttct tccttctgta tctgttccac ccgggtatcg atcacgccgt
     8761 cactcacccg gatccgcagg cgctcccctg cttccacctg ggtcacggaa cggatatttt
     8821 tcccatcggt atcctccaca taggaaaaac cactgtttaa cttttccaaa ggagagaccg
     8881 ctttcatcct ctcaatatac aatgccagat tatgtctgcg ttcccgcagg atcgcctgca
     8941 gccggttctg cagccggtct tccagctgta tgctgtaagt cctcttctcc cgtatccggc
     9001 tggcggggct taaatatttc aggcgcattt ccatctgatc cgcctgctgc ctcttatgct
     9061 gaagcactcg tcccatccgc tgatacagcc tgtcctgtct gtccaaaatc tcattctcaa
     9121 tatcactgac ctgtgtcact gccagttctg ccgctgccga cggggtcggt gcccgcagat
     9181 ccgccacata atcaatgatc gtggtatccg tctcgtgtcc aaccgccgaa ataatgggaa
     9241 cagagcagtc gaatactgcc tgtgccacca ttctctcgtt gaacgcccac agatcctcaa
     9301 tggagccgcc cccacgtccc acgatcatga catccacgcc ctgtctctcc agtgcacgga
     9361 ttccgttcac aatggaagga gccgccgaag ctccctgtac cagcgcagga tacaaaataa
     9421 tctgcacgaa gggattccgt ctggaggcga tctgtatgat atcccggact gccgcgccgg
     9481 tatctgccgt gactacccca agagtatgga tatacttcgg aatctgctgc ttatactccg
     9541 gagcaaacat gccctcttcc gccagttccc gttttagttg ttcgtatttt tcatagaggg
     9601 ctccggcgcc gtccagcgtc acttccttca catacagctg atatctgccg tcacgctcaa
     9661 atacactcac actgcccttc gcaattacct gctgcccttc cgtcagccgg aatcccagac
     9721 ctctccggtc tccggcaaac ataatgcagt tcagggcact ggcagaatcc tttaacgtaa
     9781 aataaatatg tccagaggga tggtatttac agttgcttac ctcacccttt acagatacca
     9841 tctgtaaaaa gtaatcctgc gtgaacatat ttttgatata agtattcagt tgtcgtacgc
     9901 tgattacgtt ctgtatccta ctcaccggat ttcatgatct tggcaaggat cgcattgacg
     9961 aatccgccag agctttcctg tccgtacttt ttggcaatct cgattgcctc gttaatagca
    10021 acgccattcg ggatatcgtc atcatagcgc agctcatatg caccaagacg cagtactgca
    10081 agctccacct ttcccatacg ggtggtatcc cagccttcgg tattctcatt caacagcctg
    10141 tcaagctcgg ggattttctc cctgatcttc tcaaagcggg ccgcaatggc atctgcatcc
    10201 tgccaggaag gaacttcctc attattctcg cggaacaatg cgatctgttc cggcatatcc
    10261 tccggtgcat gaaactccac gcggtataaa agtttgaaaa cctgttctct ctgctcgtgt
    10321 cttcccataa tatcttcctg taattcctct t
//
LOCUS       DAWDHD010000082        10180 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_395_length_10180_cov_13.977580, whole genome shotgun sequence.
ACCESSION   DAWDHD010000082 DAWDHD010000000
VERSION     DAWDHD010000082.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 10180)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10180)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10180
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_395_length_10180_cov_13.977580"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4069..4168
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2781 a         2462 c         2045 g         2792 t
ORIGIN      
        1 tggagaacac catggtcaga ggattgaact tggacatcca ggataccagt acagcagtaa
       61 atccctgtcc acctgcaatg gtagtggtaa cagtatggtt gataccgcct accagaagca
      121 aacctaccag tccgcacagc gcaccggaca gaagcatagt acggatgatt accttttcta
      181 ccttgatacc cacatatttt gcggtatttt cactctcacc cacaacggag atctcatatc
      241 cctgcttgct atagttgaga tacacataca tgaaaattgt aatcactact gccactacaa
      301 tacttaacag atactttgaa ccaaatatct ggggcagcca gcccacgttg gtattctggt
      361 tgatgatacc gatcttaccg gatcccttgg gaacttccca tacaatggta tagaatgcca
      421 caagctgggt agctatgtag ttcatcatca gtgtagacag tgtctcattg gtattccatt
      481 ttgccttgaa aaatgcagga atgaagcccc agatggctcc tgctgccagg ctggccacga
      541 tcatgcacag gatcacaact gcacccggaa gcttgtcccc caggcagatc atgcaggctg
      601 cggatgccag acccccgatc aagacctgtc cctcaccgcc aatattccag aatttcatct
      661 tgaatgccgg agtcagtgcc agtgcaatca acaacaggat cgcaatattc tgaaaagtaa
      721 cccaggtctt acggacggat ccgaaagctc ccagaataat agactgatat acctgtaaag
      781 gattgattcc cgtggtgatc gtcgtaatga taccgcacag gatcagtgcc agcaggattg
      841 ccaaaaatcg aatacccaga gatttatacc agggaagcgc atctcttttt acgatatgaa
      901 acaaaggctc tttattcttt tccttcatgt tctttgcctc ctactctggt catcagcata
      961 ccgacctcat ttttgtctgt ctttctcgca tccagaatac cgctcacttc tccgccgcac
     1021 aataccatga tacggtcgga cagctccatc agcacatcca gatcctcacc tacgaaaatc
     1081 acggcaacgc cctttttctt ctgttcattg atcaggtcat atatagtata cgaagaatta
     1141 atatccagtc cacgtaccac ataagccgtc aacagtacgg taggtgcaga cgcaatctct
     1201 cttccaacga gaaccttctg tacattacca ccggacaatc tgcgtaccgg agtggtgata
     1261 cccggggtta caacctccag attttctacc acatcagttg caagctgttt ggaaggcttt
     1321 cggttcgtaa acggtgtatg tccctgtcgg aaggaacgta acatcatatt atcggtcaga
     1381 tccatattgc ctaccaggcc catgccaaga cgatcctccg gtacaaaaga aagcgataca
     1441 cccatctctg cgatcttcaa cggatccttg ccgatcagca tttccttact tccatcatct
     1501 tccacatagc tgattgtgcc ggcttctgtt acctgcagac ctgcaatggc ttccaaaagc
     1561 tccttctgtc cacagccgga gataccggcg attcccagga tctcaccact attggcggta
     1621 aaggagacgt ccttcagctt aacgatacct tcttcgttac gtacggtcag tccctgtact
     1681 tcgatacggg gcttcggatt cacaggttcc ggtctgacaa tattcaaagt aaccgcatgt
     1741 cccaccatca tattggtcat ttccgtaacg gtggtatcct ttgtcagcat gcttccaata
     1801 tactgaccgt tacgaagcac agccaccctg tcggacagag attccacttc atgcatttta
     1861 tgggtaataa tgacaatagc cttgccgtca tctctcattt tccggagaac gttaaagagc
     1921 ttttctgtct cctgcggtgt cagtacagcg gtaggctcat ccaggatcag gatatccgca
     1981 ccacgataca gcacctttac aatctccacg gtctgcttct gggatacgga catatcatag
     2041 atcttctgct tcgggtccac ttcaaaaccg tatttattgc agatctcctc gattttacgg
     2101 gaagcttcct taatgttcag tccaccttcc agacccagaa taatattctc ggtggcagac
     2161 agtacatcga tcagcttgaa atgctgatgc accataccga ttcccaaccg caatgcatcc
     2221 ttgggagatc tgatgacctc ttccttaccg ttaataaaga tctgcccctc atccggaaaa
     2281 taaataccgg agagcatatt catcagtgtc gtcttaccgc tgccgttttc tccaagcagt
     2341 gataagatct cacctttgta gatatccaga ttcaccttgt cattggcgat cacggatcca
     2401 aaagtcttgg taatgttttt cattgaaacc gcagctactt ttgtctcttt ttccatcctc
     2461 tgtcaccttt ccaaattatc cataacctgt atactttgag tcgccccttt tctaccctgt
     2521 caattcttac aaattaacag ggtagaaaaa gggcggcgcg gcgataaacg cggcgccgtc
     2581 caggcttttt aagaattaat actgctcgtc cagcaggttg atgccgtcga ttcttacatt
     2641 gaagtaagga gcggaacgga atgcaacatc ggactcatgg aagtatccgt cacttactac
     2701 ctcatgatcg ggagtataat caggatcggt atcgatatca gcctcatagc tgtccagggt
     2761 cttgccatct acagtgaagg tagtgatatc atatacgtga gtcttgcctg cgatcagatc
     2821 agcctttgca gcttctactg cttctgcagt accctctgct acagcagcac caagatcact
     2881 cagctctaca gatccggtct ccagagtacc acaccaatcg gtatcgatag gagttccatc
     2941 tgctacacat ttaatagcat actcaaaata aggctcccag ttaactctgg aagataccag
     3001 gaaggtattg gggcaggctg cctcagtgct tccattataa gatacgttag gaacacctgc
     3061 ggtctcgcat gcggtaggag cacccatgga gtctgcatgc tggctgatca gtacgcatcc
     3121 gccctcgatc agcttgttag ctgcttcctt ctcagcggtc tcatcatacc aggaaccggt
     3181 aaaggttaca tccatggtag cgctggggca tacggaacga gcgcccagga agaaagaggt
     3241 ataaccggac ataacttcag cataggtata agcacctaca taacccatct tagcctgatc
     3301 ctcggtgata tcaccgttag cgatcatctc attcagcttc atacctgcaa cgatacctgc
     3361 aagataacgg ccatcataga tggaagcaaa tgcattgtgg aagttatcaa ggccttctgt
     3421 atgagccttg gttccggttg catggctgaa ctgaacatcg ggatattcct tagcggcctc
     3481 gatgatgtag tcctcatgtc cgaagctgtc tgcgaaaatg aaattacaac ctgcatcagc
     3541 aagctcacaa gctgcttcat aagcttcctg tccttcagga atgttggtct tggtcatgta
     3601 ctcaatacca agcttctcac aagctgcttt tgctgcattc aggaagttca gatcataagt
     3661 agagttctca tcatgcagga aaatgaaacc tgcctttaca ccggtaacag cttcactgtc
     3721 ggttgctgct tcggtagtac ctgctgcttc ggtagtacct gctgcctcgg tggtagctgc
     3781 aacggttgct gcatctgtgc cggtggtagc tgcatcattg gatccgcagc ctgccagagc
     3841 agtaactgcg agagccatag ctgcgagtaa acttacgact ttctttttca taattctcct
     3901 ctttctgcgc tgctgcttcg ttacagatcc atgtagcagc gcgggcacag atctgcagat
     3961 aaaccggtga gtcaacgcaa aaagggtgta tgacaccttc atacgacccc tttattgtac
     4021 tctctcaaca ttagtcagta taagcatttg tctgacttct tgtcaagann nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnac attttgctat gttaaccatg agaatactga
     4201 aatcacgagg tgccttatgc ctatcagagg atatatcata gaaaaatata atactatgac
     4261 taacgcttat acctgcaacc gcctggtaca ggaagcttct gccctggata tggatctgca
     4321 gattgtaggg atccatgata ctgtggtctc tccgcatggt gttataaatc atggtaaaat
     4381 actggaaccc gtggattttg tcatcaaccg ctataaatgg ggacgggaaa aggatgctat
     4441 aaatgcactt gccacccgaa gctataatcc attaactgct tataatatct atattaataa
     4501 atttgaacag gtacgaaggc ttcattccaa agcctttttg atcccaaaat atcttctggg
     4561 aacttcacgg ttgccttttt cttctattgt agaacaactg ggccttccct ttgtcggtaa
     4621 aggactggaa agctccatgg gagaagaaat cgtctgcatc cgggataaag cttcttatga
     4681 ggaactctct cttaactacc cttcctccaa ggaatggctc ttcgaagaat ttatctcaga
     4741 aagctacggc agagatctgc gcttttacag catccgcgga gaagccgttg cctgtatgca
     4801 gcgcacatcc cagggggatt tccgtgcaaa tgtagctctg ggcgcttcgg taaagccttc
     4861 ccctgtcact tctggcatcc gtaccatagc cgcagatatt tacgagcaga ccggactgga
     4921 ttttctggga attgatctgc tttttggcag agaaaaacct tatttttgcg aaatcaatgt
     4981 catgccggga ctggaaggta ttgaaaaggc ttccggcata aatgttgcgg gaaaaattat
     5041 tgagacgata caatctgact ttacataaaa gacttagtgt aaaaaatagt tttttcatgc
     5101 gcaaatatgt gcctgcacag tgtacgaaaa tacatgatac taaagtatct ggaaaattgc
     5161 aggctacaaa aaggtattta tatgaataac acttccgaaa catcactgca atttcataaa
     5221 atcactacgc gtaaggaagc ggaagacttt gtctatgcct cttatctgcg agctgcctca
     5281 catcaggatt atgctgcaaa ggccgccaga aaacgacacc cggaactgac cagatccctg
     5341 ctttggcaaa aatccgttac tccctgcatc gttgtcaccg gaagcaaggg aaaaggctcc
     5401 gtttccaaca tgatctcccg aatcctgaag acaagattct ctgtgggact gatgaccagc
     5461 cctcatatca cggactttcg cgaacgcttc cgtgtcaatg atacgatgat cagtgaatct
     5521 gacttctgcc gtctgatgac agagatccag ccagagattc tgaatattgc ctctgatctg
     5581 ccggagtcag tatgtatcag tcccatggga atccaggccg atctggcact ggcttatttt
     5641 tcggaaaaac atacggattt caatgtactg gaatgcggta aaggtgcaaa atacgatgat
     5701 gtgaataatg tacgtcacaa ttatgccgtg atcaaccgga tttttctgga gcatacgagg
     5761 gaactgggtg atacgctgac tgccattgcg gaagataaat cccacgttat caccggagag
     5821 cagaagtgtg tctattttgc ggagcaggaa ccggaagttc tggaagttct gcagcgccgt
     5881 gccgaaactc tgcatattcc ctataagatc tacggtcggg attttcatgc ggaaaacatc
     5941 cgttatacct gcttcggaat gcagtttgat gttgaaatcg gagacaacat ttatgcggat
     6001 ttacaaattc cgttgctggg agaacatcag gcgaaaaact gtgctctggc acttgctgtg
     6061 tgtgtggatg tgctgtctga tctgcgcagg gaatccgcag ttttttcttt gccagacatc
     6121 cggaaaaatc tgtctctgct gcactggccg ggacgcatgg aagtcctgcg ttccaagccg
     6181 tttgttctgc tggatgcctg tatcaatgcc accagctgtg agaatgtaaa ggaagtcctg
     6241 cgacatctgg gggtttggaa ttgcactgtt attgtgggaa ttccggagga taaggattat
     6301 gccggggtgg ttcggtccat gaaaggtatg gcaaaccgga tccttctcac cagatctggg
     6361 aatccgcatt atcatttttc ccaaaaacag caggagacat tggcagaaga agatattggc
     6421 acaatatgga cagattccgt gaaacaggca ctgactctgg ccggttcttg tccggatccc
     6481 attgtaatct tgggaacgac ttcggtaatt tccgaagtag agcagatttt tcagcaatct
     6541 taacttgccc tcaccggata caggtgctac aatgagggca aatagaagag tagcaaaaca
     6601 ttacgcagaa aggagaagtc ccatgacagg tatttacgga atcaattatt ctaataatta
     6661 tagtctgacc ggcaattacg gatacggtta ttctaccagt gcggtatctt ccgcacagcc
     6721 cgaagagaaa ggtatggtgg taaatcccgg aaaatccgat gtagaaaagc ccggcagaaa
     6781 gtcttctccc gcagaatgcg aaacctgcaa gaaccggaaa tatcaggatg gttccaacga
     6841 aatggtctcc ttcaaatctg catctcatat ttcaccacag gcttcctcct ccagagttcg
     6901 tgcccacgaa caggagcatg taacgaacgc ttacaacaag gccgcacaga acaacggcaa
     6961 ggttctctcc gccacagtgt ccctgcggac cgcggtatgt cccgaatgcg gcaccgtcta
     7021 caccgccggc ggcaccacca cgaccaagat cagttaccgt gacgagagca atccttacca
     7081 gcaaaataag aaatctgccg atgctgccat gctgattgga cggaatctgg atctgagtgc
     7141 gtgaatgaaa cataattttt gacacgcgaa agcccccacc ttttatgtga gggctttttt
     7201 attttcattt taatacgcca cataccagtc ccggcagggc aagagagtga atgcatcgaa
     7261 tttttccgca tctgtatagt aaatgatata tatcttattt ctgtcgatgt gctcccagtc
     7321 gattcccatg gtgaagcgga ttccttttcc cagaaaatcc ttcggcttgg gaggtacatc
     7381 gcttaagttc ctgttggcaa ggtattctgc agcgtcaatc tctccgtaga gcaaaacact
     7441 gggatatttg atggcatctt cgatctcgat ctcacgaccg ctttccgccg caatctccat
     7501 ggctttctgt acggcttcct cgctgccttc ctgaaaatag gcactgacca gctctttata
     7561 agaagtaaaa tatgcctttt caaactgtac attttccccc agcagaagtg ctgccaaaag
     7621 aatgcttact gcaatttttc catagaaatt cacttttttc cccagaaaat ctgtcaaaga
     7681 agacacgcac aatgctccgc acagaacaag aggaatatag atgcagttga tctgggtcat
     7741 tcccacggta accagtaatc ccagcagcaa tgcgccggtc agttgaaaga atatcatcca
     7801 taataatgtt gtctgtctct tctgtaaggc taggatcagg cggatcacca gtaaaaaaac
     7861 tcccagaaag atcagtactc tggctgtgat tccgaaaaat ccaaacggca tgatcacatc
     7921 gtagggacga ccgatatcct gccgatacag aagatacagc agatttttga aattgtataa
     7981 aatatcacgg aaagaggatg ctatttcatt cactctgaaa tccgtcatta catagaccga
     8041 aaaagggcct atggagaact ctgggaggat tcctctgttt accagtaaaa acgccagcaa
     8101 cggaagtgcc agtaatccaa tgatcaccac atggatcacg aagtatttgt tgatcttcaa
     8161 agtctttgta agaaatccgt atccccattc taacaacaga aggagtggaa caataggcca
     8221 gatggtcgca taacaataca ggctcagacc gtaagagagt gctgacaaaa taagtaatct
     8281 cggttttttc agaccatagg caaaaaaagt cagtccaaaa agcaggaacc cgggtgccag
     8341 attggactcc agcccccatc ttgacatcat aatatgccag ggacatacac tgagaagaag
     8401 catggcccac agtccggccg cttcatccac catctctttc atcagaaaat aaaccgccac
     8461 cagagataaa attgctacaa tggcctgcgg tagtcttact accagtggag tgatcttacc
     8521 tccggtgaac accataaaag gaagcatgag atacgagttc agcgcattct gtccgcctcc
     8581 ccaggactcg aaatacacag gataggagtg tcctgtggaa tcgatcccac tgtgaaacag
     8641 tgaatatgca ttgaacgctc cataagcctc atccatctga tagccaccag gtacgtttcc
     8701 aagctgcacc agccgcaata tagaaccaaa tactaatatg agacaaaaca atatggtaga
     8761 tttatgtcgt tctattgctt tctttaatgt cataggtctt ttatatcctt tttactttcc
     8821 aatatccatt tgggatgaat attattttct tctgtttttt attttatcat atgggatatt
     8881 ggattgtctc tgtaaaagtg aaaattactc tctttccacc tactgtggta aattcaaagt
     8941 tggatttttc aacagtgtgg caataaccgg ggtatattta gatactccat agtcttcaac
     9001 aatactggtt attctagccc ctgcatcctt tgatgaagca ccacataaga aaactctttc
     9061 atcagtagtt ccataatcca acactataaa tcgatcatga aatataccgc ctgtcttttt
     9121 cattgatatt tttactgctg gatattcttt gcaaaaatcc gtaaattcta tattatgaag
     9181 tttattattt ccaacattat cactgaataa aataatagct actccagccg gagaattctt
     9241 aaggtgtacc agcgtcctta agccaatata attgtcaacg acatatattg acgactttgc
     9301 ctgcttataa atggactcat aaacttcatc cgcactactg aactttgcat taaacatgag
     9361 ccacttctcg tcatcatctg aaacaaagct gttcatcatg tcagctaact cagattttgt
     9421 cacaacatcc ttcaatcctt ctgcaacatc tgaaatctgt ttttccaaag atgtcatatt
     9481 tgaattgatt ttattaattt ctattctgtt gttagcagtc tccatgctta actgaagtat
     9541 ttcccgctga cctattaaat cacgattttc taatatgtaa tctttcattt tcttaaaggt
     9601 tcttattaat gccttgctct gtttaacagc caacggtcct ttcaagacag tcatcagcat
     9661 atataatcct tgctctgtaa atacatgagg attataacga ctttttgaac ttatatttgc
     9721 ggtacaattt ttgtaccgca aatcttctaa ttcctcatct gtcaattcaa acataaaatc
     9781 ttcatcaaat ttttcaatat tattttttac ctgtctatta aatcccttcg tatcatagcc
     9841 atatatctct gctaaatcag catcaagcaa taccctttta cccctaattt tatacagttt
     9901 ttcttttaga tattcttctg ttacttctat tactgtaaca tcgatttctt tctttttatc
     9961 ttctgccata gaactcctcc atccatttgc ggtcaaaaaa atcgtccgca actctcatat
    10021 aacatattgc tgacatcagc aaaatggtct attcctcttt ttccctcagt ttgtcataat
    10081 atgctttata cctatagatc gtcctctcac tgacattgtt cctcttcgca atttctacca
    10141 ttgtcatgcc gctctcatac ccaacaacaa aatcattata
//
LOCUS       DAWDHD010000083        10148 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_397_length_10148_cov_11.476568, whole genome shotgun sequence.
ACCESSION   DAWDHD010000083 DAWDHD010000000
VERSION     DAWDHD010000083.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 10148)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10148)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10148
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_397_length_10148_cov_11.476568"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3688..3787
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2381 a         2633 c         2046 g         2988 t
ORIGIN      
        1 cctgcccgtt acggatctgc gtcttcagtt ccgtaatgcg gtccttgctc tgactgagcg
       61 atccggtcat acgagagggt gacatgctgt ttaacagatc gttcaaccgc ttgtccgctg
      121 tctcaaaatt attgatatcg tccgtattct ccgccagatt cccgattttg gcattgatgc
      181 cgtccgtaac aaaggtctcg cagtcattct gtgagataaa aatcgttcgt tcaaaggatc
      241 ctgcgtcgat ccggaacaac tcttctccca gattttgtgt aaaatctctg catggcagat
      301 ttgttttcgt atctgtcagc tgaaattcat cttctgcgct tttacttccg aaggttctgc
      361 ggacagaata ttgctttcct tcactttcaa attgcagttc tccaccatag acgccctttt
      421 gccagggact gtatctcttc ctctcatttt ccagtgggtc cctcttccct tcattccgga
      481 atccgaacag cataacgcgt aagaatgcag ccagtgtgct ttttccccag ccgttttcct
      541 cacagacggt gttgcagcca tctgtaaaat catatgtaaa gtcacttagt tttccgaaat
      601 tctcaatgtg acatttgatc agtttcatgc gtccaggatc tcctctccgg ccagtgcctg
      661 caatccgtaa tgtatcactg tcaccttgtc ctcttccggc agatccgctc tgcccatgac
      721 agtacgaata aactctccct ttaaggactt atcctttgta aaagcagcat aatccaccag
      781 atactcagag ctgtccttaa tcttcacaaa ataaaaaagg tgactgaact gatgtaacag
      841 aaagccaata tccttttcac tttccacatc tattttcccg accagttcat acttgatcag
      901 acttttctcc gggtaacctt tcccggctgt ctcggctctc atccgttcca tgatttccgt
      961 cgtggattca caaccggtaa tatccaccgg gatggtatag agcttccggg atgcgaaagg
     1021 aataaacgta ctgtcaattg tacctgtcag ctcatcaata tcaagcatca caaatccatg
     1081 ctctccgcat tcgtcaaacc ctctgccctc cagacagccg ggataacaat agacgcctct
     1141 cgcatccaga cgctcttttt tatagctgtg gatgtgtccc agggcaagat aatcaattcc
     1201 cttatttttc aatgccgtca gatgaatgat ctccgcctta tctctcgcac tgtattccga
     1261 ttcctgccca tgcaggacta caatgttgca ttgatccaca tccagtgaca gcgtattata
     1321 tatgcttcca ctgttttccg gtgtcagttc caccccagta accgcaactc ccttaccgga
     1381 ggtattctcc gtccctgaaa gttcatagct tgtccagttg ttcccaaata gatgaagatt
     1441 ctccggaagt tccgaaagac cggaagtaaa gcttccttcg tcatgattcc cctgtaagta
     1501 ataaaaagct attttgggat gtccctgaat taagtctttt accacattcc gggcggtagc
     1561 ggatacgttt ttcgtatcga acagatctcc tgcgattatg atcgccgcca cgccctgctc
     1621 tgccccgtag gtaaccatat tctgaaaagt agtcagcaat tccgtcttgc gttctctcgc
     1681 tttttctttg gtcagatttg cactcatttt ggaatccaga tggatatccg cacaatggat
     1741 gattttcata ttgctgcctc ctgtattatc tttgtagtat atgtcttaaa atattctcat
     1801 tcatgcgcac atgattcgct ctttatgaaa attaccctgg ctctgaatag ttacaaaata
     1861 tcttaaaaca atgtcgtacg gatctgcatt tgcagacact tttattatat acgaatctct
     1921 gttcgaatac aacaaaaacg tatcggcaat gagacattca gtctcatttc ccgatacgtt
     1981 ttttaactac ctgtttccgt ccagtttcag taaatccgaa aagctttgca aggggtatgt
     2041 tacgaaagca gatgttgctg cttcccctaa tcctgcactg tcaaaacctc cctcttttgc
     2101 tgccagaata ccggcttttg catcttccac caccaggcac ttttccgggg gcagccccag
     2161 atactgtgct gcacacagaa acacttccgg gtccggtttt gaccttgtaa tattggttcc
     2221 gtcgctgacg gcatcaaaat atccggtaag cccaagcctt tttaagatca gtttggtgtt
     2281 tttgctggaa gatcctattg ccaattttcg tccgcggctt cgcagcgtat ccatagtctt
     2341 ccggacttct tcggacaaat ccgcctcgcc catattttgc agcagaccta catataattg
     2401 gttcttcttt tccgcaagtg ctgctttttg cgctgcggtg agcggttcac cgtgatattc
     2461 ttccagcaaa atctccagac tgtccatacg gctcaccccc cgcatccggt cattgatccg
     2521 gtcatcaaaa ttaattccga gttgatccgc tatctgtttc catgccagga aatgataccg
     2581 gtccgtatgg caaatgacac catccagatc aaatattatt ccctgatagt ttttcctaca
     2641 catgcagact gcctgccgta atcagtttgc ctgacttcct gtcaaagagc aggatatcct
     2701 ctccgccaac ttcgaatttc acttcctgtc cgatcttgta tgctgtattt ccaaatacta
     2761 caccggtcag caggaaatca ccgatacgaa gcttaatcgt tgattccata ccggtgggca
     2821 tggcaccgta gatcacgccg tccagacctg caccatcgtg taattgcagg gcttcgggac
     2881 gtacaccgat gacaaaatcc tcgttagtga tcacgggagc ttcctgcaat gcataatcgt
     2941 cttcgttcac ccttgcaata tgatatttga aaacttcatc tttattcgac ttttccacag
     3001 atttcttatc ctgcatctgc tcttggtgat gcgctgcctc atcggcttcg ttcttatctc
     3061 tctcagcgaa ccatctctgc agatccaggt gttctttcgg aacaaacttt gcctttctac
     3121 cgtctaagat tgtcacatcc aggctgccgt tttcattctg cacacccctg gcttcgataa
     3181 aattaatgga aggatttcct acgaaatcag ccacaaacag gttgttgggt ctgctgtaca
     3241 cggtcaacgg tgcttcatac tgctgcaaca caccgttgtt gatcaggcag atcttcgttg
     3301 ccagtgtcat agcttccatc tggtcatgcg ttacatatac gaaggtgctt ccggtctcca
     3361 catgcagacg ctgtaactca tatctcattt ccagacggag tttcgcgtcc aggttagaca
     3421 gcggctcatc catgaacaat acctggggtt ccggcgccag tgttctggca atggcaactc
     3481 tttgctgctg tccaccggac agttctgcag gataacgatt catgaacatt ccgatcttaa
     3541 cgatacggga tactctgcgt actgccaggt caatctcttc cttggaatat tttctgaccc
     3601 tgcttactac cttgccaccg gatacgactt cgaatttctc gttaagttct tccccttttt
     3661 ccttatgacc tgccaggatg ctgtccannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnntcc tcatcgatca cgggaagttc ttccttgata tttcccagtc cgaaggagat
     3841 gttctggtat accgtcatgt tcggccacag tgcgtagttt tggaacagga atccgacttt
     3901 tctcttattt gcgggaacat tgatccccgc ttcactatcg aataccaccc ggtctccgat
     3961 cttgatctgc ccgctggtag gggtctccag accggcgatc atacgtaagg tagtggtctt
     4021 accgcaaccg gagggaccaa gcagagtgat aaaggaatta tcctccatat ccagactcag
     4081 atcatctacc gcatagaatt ttccccaacg ttttgtaaca tgtgttaatt tgatttccgg
     4141 catagttatt tacctccgat accttcatcc aggctggacc cggtcagttt atttaccaaa
     4201 gtgttaaaga tcaggatcgt tacgatcagg atcaggttga tggcgctgga gaatgcatac
     4261 aatcccatct catcgaaata atccagtgtc gtggagagga taaagccctg cgtacataac
     4321 agtaagaaca gggacagctc tcgcagacag gtcatgaacg gaagcatata tccgttgata
     4381 atggaagact tctggatcgg aatgatgatc cgcagcatcc gcttgtacca gggaatatcc
     4441 tggatgatgg ccgcttcctc gatctctccc gacagctgca gcatggagtt cagtgcactt
     4501 ctgctggcaa agggaatgta cttgatcacg cctgcgatga tcaacaatgt gtaagtgttg
     4561 aacaggttga tcttgtcatt ggagaacagg atgaagaagg ctgcgcccac tgccagggat
     4621 ggcatcaggt acggcaggaa tgccacaccg ttcacgtaat tggcccattt acttctccgg
     4681 ttcttggata ccgcataacc gatcagggta ccgatggtac ctgcgatcag agcacagcag
     4741 acggcgacta acatcgttcc cttgaaagct ctccagatca gcggattgtg aagaataccg
     4801 ggctgtccgt acagagcacc ggctccgttg ttatccgtca gccaccactt ggtggtcagc
     4861 gcactggaat ccatggtata caggaaactg taatctcccg gattcggcag gaaagtctcc
     4921 agtgcgaagg acaggatcgg gaaaatactg gtgaagaagg tgatgacgat caggatgacc
     4981 gcgatcagat attttccgac ttttcctaag ttgatcttgg agatctgtcc agatttaccg
     5041 gtaaccgtcg tataattttt acggctcttt aaactcatct ggttcattaa caggatcgcc
     5101 acaccgaaga tcatcatgat gatggcaaga atactggctt ccccggtata cttggagttc
     5161 atggagatgt acttggtgga cagagtggtc agtcccagat agtggggtac gggataactt
     5221 cccatggcgc ttccgaatac cagcaggatc gtggatagaa ttgccggctt taccataggc
     5281 agtgtgatcc ggaacatggt cttccacttc ggcgtatcca ggatggttgc cgcttcctcc
     5341 agattggcat ccatgttacg gaagataccg ccgatcagga tgtaggcgaa gggggcataa
     5401 tgcaatccca acactaccgc gctggggaac agtcccagac accaccactt cggaaaagca
     5461 attccaaaga gggatgccag cagtccgttg gaggttccgg taactgcgtt actgttaaac
     5521 acgttctgcc ataccacagc cagtgtccac tgcggcatga tgtagggaaa aataaaaatg
     5581 gagcttaaat attttttaca ttttaaatta gttctcgtaa ttaagaatgc gaacaaaccg
     5641 ccgaacaaaa tggagatcag gcaggtaaag actgccagaa gcacggtatt taacagcggt
     5701 gtccacagat ttgttctcgc cagtttactg gtgaacagat ccacataatt ggcaacggaa
     5761 tatccgcctg cctttccggt aagatgtgca tcgatggtgc ccggatggat cttgaaggta
     5821 tcctcgacga ttgccacgat cggtgccact gtggtaaagg taagtacgat accaaacaga
     5881 agcagaatga tattttgcgg cttggagaaa tacgtcttga tcttgttcag aacaatcacc
     5941 atcttgcttg ttttttttgc ggcactctga ttcatggtta acctctcttc ccaatacaag
     6001 cttatgtcct gtagaaccat tcatccctgc gaatcagaaa actgctactt acatgcaggc
     6061 atgatgtagc agattttctg attctgacag gacgaatagt cactctttta tatcatatat
     6121 cactcagcag tatacttcgg caacagctcg atccagcttc ctaccgtgaa ggatacggat
     6181 gcgcagtact caggatcttc cagcaccagt tcaccgtcgg tagaccacca gtcaaagcct
     6241 ctgtcgttct tgctctcata taccacatta ccgttctcat cgttaccacc ggtgctgtgc
     6301 tgataggtag cttcgattgc tttggcgact tcagggtttg cagaataacc gcccatgtct
     6361 ttgccccatg cgctgaagcc gtcttccgta caggtcatgt atgcgatgaa agcacatgcc
     6421 gtccagggca gaggactgtt atcagtaacg aacaagtaat gacagtagcc gtagccacca
     6481 attcccttat agccatcctg ataagcggca accttaatat tgttcacaga tacacctgcg
     6541 gactcttcta cggagcgcag cttactgtat actaacagtc cacactgatc ggtagcggaa
     6601 tcggatacca gggtattgca gataggtccg tcatcggtct gggcattgta gctgcctacc
     6661 cagagtttga tccatgccag ggcatatttt ccatcggcgc ctaagcctaa atcatttgct
     6721 tcggattcca tctcatcaat cgtgggctgg aagtaagcct gctcatcagc gggtaatgca
     6781 ttaaaggcat cctttaacat ggctgcatat ttgtcttcgg tcagcatgta caggaagttc
     6841 ttacctacaa cttcagaatc aatatccatg aacagtgcat gggtatcttc tgccacgaag
     6901 tcccagcagt tgtcatatac tttgctgccg gtgcagttat attcgaatac tttattcaag
     6961 gtctgtaatg caaggtaacc gtccactgcg tcgggagtgg ttccgttggc ttctgcccat
     7021 tccttaggaa taaaggtatc cagtattccg gtctgtgtca tcttggattc gatctgatta
     7081 ccatcctgga tcagagtcat tgcgaaggtt ccttccggtt tcagggaatc tgcggtcagc
     7141 tgctcgaaaa tcttgttgtt cttgggctgc tgccagtcaa attccatgct gtaagaagga
     7201 tcaatagact gcagatactc aataaacagg ggtaatgctg ctttaccgcg gctggagtta
     7261 cccacaccgt agaaggtctt gccgttggat tcctctatgg ctttctgtgc cagttcttcc
     7321 agggtcattc cttctgcctg tttgatgatc tgctgagttg cagtaagatt tgcgtcgtcg
     7381 gctgcctccg gggtcttggt ctctgccacc gtctcggttc cggctgtctg tgtgttatct
     7441 gcgcttccag ttgcctggtt ctgtgtctcg ctgctgccgc atccggctaa cataccaaca
     7501 ctcagtgcac agatcataag taacgatact acttttttct tcatatgctg tcctcccttg
     7561 aaacatatag tttgtataac ccttcatggt ccacttactt ggaatgggat gtttcccact
     7621 tccgttctga tgattctatt atacgttggg agttcagcaa tttgtataag gtaaaactgt
     7681 tattatttta atatttctga tattatgtat aatatttctg ctattttctc gattcgtttg
     7741 tgcaatctgt tatctcttca ggcttctatc ttgatattcc gaaggtgtta caccgacaat
     7801 ccgcttaaag ctgctggaaa aatgtgcaat atcacgatat cccacctgct ctgccacctc
     7861 atagacttta tttctgcagt catttaataa tttcatggct gtccgcatcc gcactctggt
     7921 aatataagcc gccaccgtat aatccgtctc ctttttgaaa gtatgactta aatgtccctc
     7981 gctgattccc acatgttctg cgatctctga cacgcataac tcaggatttt cataatgttc
     8041 ggtaatataa tccactgcgg ccatgacgta tttgctcttg tcgcctttgt tcagtaccaa
     8101 ctccggaagc tgatcatcgc cagccttttc ctcacgttcc tgtgtctttt tccgtttatc
     8161 caatgcatta ttcactgcct gctccagctc tccgtccccg aagggtttta acagataatc
     8221 acttgcaaac agcttcaggg cactctgtgc ataggaaaaa tcactgtatg cggttaaaaa
     8281 aatcacactg acatcattgc cttctgcccg cagttccttc agcatttcga taccgtccat
     8341 tttgggcata cggatatcgc agatcagcag atccggatga tatttccgca cggcttccag
     8401 tccttcgatt ccgttgccgg cttccgccac tactacgcag ttcagtgcct gccagtctgt
     8461 ctccatcacg attcctcgtc gcaccaacat ttcatcatct attacgatta ctttgtacat
     8521 aacccaattc ctccctgtct tttgcaccat atgtcttttg tttttctttc cttttcgtct
     8581 ttcccctctg aatcactctc ttgaattatt ctctttgctg cttccttttc aggctttttc
     8641 tcttctgacc ggcagtcgca atgtcaccct ggtacctccg gattccttac catcctttgt
     8701 agtttggata tttccatccg atttttccat gtaaataccg tattcctcac cgtaagtcag
     8761 cctgataatg gtgtcaacat ttgatactcc gatatgtccc acaagctgct cctgtctcct
     8821 gtgattgatt gcctccatta aatcatccgg agctccgcag ccatcgtcag tcacctcaat
     8881 gcagagtaca ccggtcttcc ctgtaatccg cacatggata tttccttctt cgctctcttt
     8941 taatccgtgt aacactgaat tctctacaat cggctgaata atcagtttcg ggattacaca
     9001 atcttccagt ccttcttcca tttccacgga ataggaaaac ttatcattga aacgaatttt
     9061 ttggatgtct acatagcaat tcaccagctc cagttcttcc ctgagacaga taaactgcct
     9121 cttggagata ctggtccgca gaatcttggc caatccggct gccatggtcg caatctccgg
     9181 aatatgattg gcctttgcca cccatttcat ggtatccagc gtattgtaca gaaaatgggg
     9241 gttcagctgc gcctgcatca tggcaatgtg agatgcatta agttctttct gcttctctat
     9301 cttctcctgc acattctgtt ccagttcatt tgccataata ttaaaattcc ttgacatctg
     9361 ccccagctca tcttctctgt cggaagcgat ccttacagtc aggtctccct cctgcaaagt
     9421 gcgcatggca gtattcatcc gttcgacggg gcgcgtcata tttctgccta catgggatgc
     9481 cacacctaca cacaataaaa tactgatgat cagcattacg gtaagtacac tgtacatgga
     9541 attcagtact tccgtggaaa atacacttgg acatgccatt accgtatata atccggtatt
     9601 tcccaaaggt tctatcaaca attctcctgc gcgataaacc cccagtaact tatgtccctt
     9661 catcagctct tctctaatct tgtcagtctg cagattaccg ctttcaaaaa tcattcgcca
     9721 ataggtatct aaaaaggcga cttccgcctt actgtttccg ttatcataga gcagtttttc
     9781 aaaattttcc gcccgcatgc cgataacgat gtagccctct aaggtattct ccttcccgag
     9841 cagtttccct gcagcataca tcaaaatatc tttatcatta ggctccgccc tgcgcagaac
     9901 caggttttct tcactttctt cgacggcctt cagaatgccc cagtataccg gcaaatctgt
     9961 tccggcattt ccctgagccg tggtataaat acattttccg gttttatcat acaggtcaac
    10021 ctgcgcatac tcccgtaacc ctgacacttc ccggtaaaat tgcagataaa tcttactctt
    10081 tgaccaggag tcttcttcat tgatctgccc cacgatttta ctgttcgcat taatactttc
    10141 tactgttt
//
LOCUS       DAWDHD010000084        10081 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_399_length_10081_cov_12.295931, whole genome shotgun sequence.
ACCESSION   DAWDHD010000084 DAWDHD010000000
VERSION     DAWDHD010000084.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 10081)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10081)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10081
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_399_length_10081_cov_12.295931"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3049 a         1935 c         2811 g         2286 t
ORIGIN      
        1 ggcggaggga gaatacagct atcgcgcgga gcagatcagt aatgatgaaa tgggacagct
       61 gaccgcggat ttcaaccata tggcagaagc gctggagcag aatattcaga atttggaaaa
      121 tgaagtcaga gccagagaag attttattgc cgcattttcc catgaactga agacaccatt
      181 gactgccatt atcggttatg cggatatgct gcgttcccgg aaattggatg atgagaggca
      241 ttttttgtgt gccaattata tctatacaga gggaaaacgt ctggagacta tggcactcag
      301 actgctggac atcattgtga ccagacgtaa ggagatcgat cgtaagacca caaatgtctc
      361 cggtattttc tcgtatctgc aggaaatcta tgatgagatc aagaacccag aagacagcag
      421 ggtgaaggtt gacatctgct gggaaaaggg agaactctat gccgaggaga acctgttgaa
      481 gactgttttg atcaatctga tcgataatgc catcaaagcc tccgaggagg ggcagactgt
      541 ggagatcaca ggaaggcgca tggagaatgg ttattatttt caggtacggg atgaaggaat
      601 tggcatcccc gaggaagaaa ttcataagat cacggaagct ttttatatgg tggataaatc
      661 cagatccagg gctcataacg gagcgggact ggggcttgca ctgtgcaccg ctattctgga
      721 actgcatcgt agcagactga agatcgagag tacacagggt tttggaagct gcatgagctt
      781 tctgattccg gatgaggggg tgaagtcgga tgaatagatg gaagagctgt tctttgggct
      841 ttgcggtagc agctgtggtc attcttgcgg ctctgtgggg accggaatgg atcgcggcca
      901 aaagtgatga gagactctta aacagtatta ccacagaggc ggtggaaggt gcggaaggat
      961 atcgttaccg catgagcagc aatcagaaac tatatctttt ggggagatgt ctgagcagtc
     1021 agaccctgcc ggagagtgaa ctgcgttttt tgaccagagt ggacagtgag gcaggaaact
     1081 acggagaaat gacgggtacc tatgcgtttg tagagaatcg tcagcaaccg ggagagggac
     1141 agattcagga ggaagcggta tatgaggcat gtaacagaga gattcagatc ctgaaggagc
     1201 agggaattct tcccagcgaa gtaaaggaag tttcggaaga ctcttacgaa gcggtgatct
     1261 gctctgctat cgatgttttg gagccgagaa ataatctctc tgtatggaaa atcagcctgt
     1321 ctacggacgt aaggaatgcg gataagagta accgttttct ggatatttat ctggatgcgg
     1381 ataccggcaa aatctatgaa ttctatgtcc ggaccggaca gcaatgggaa gatatcgata
     1441 cggatgccat gatcgaccgg tatgcggaat atctggaact gaccggtctt gaaaaaaatg
     1501 aggatcaaaa tccgctgttg gagacgacac cgtattttgc caagtatact ttcccggggg
     1561 aggaggaagg cagtaccacg gtgaccatcg gttactatga aggaattcgt gagcttttcc
     1621 tcaaagtagg aagatagaaa aataaaatac gcaaagtttt tttacaaaaa agattaaaat
     1681 cactacttga cgttagcaca ctaaagcagt aatataaata aaaattaaat acagcggtaa
     1741 gaaaaccatt gatgtggaga taaaaacagt tcgtgcactt acagagagac cggatgctga
     1801 gaaaggttag aacgcaggtt gttaaatgga ccacggaggg cgcagtcaaa gaggacacat
     1861 ggaattatga gatctcaagt attctgcgac gtgaaggcat acgtcagttg ccggggatat
     1921 gttggtatcc tgtaagagca caagcaattg tgaagcaagg tggcaccgcg ggaaatgtat
     1981 atgacccgtc cttgacagaa gaagattctg tcatgggcgg tttttttgta ccttttcaga
     2041 gcctatggcg agatagcctc aatgagtaaa aggaagcctc aaagaggcaa ttttacgaat
     2101 ggaggcgctg aaaaggtgtg gaaagaaata attctaagga ggagtaccgg aatgaagatc
     2161 acacccacat tggaagaatg taaaaacatc gcagcggaag gcagctacgg cgtaattccc
     2221 atcagcacag agatgtatgc ggatatgacc acacctatcg aagtgttgcg catcctaaaa
     2281 aaggtcagcg gtcatgtcta tctgctggaa agcgcggagg cagataagcg atggggacga
     2341 tattcctttt taggctatga tccattgctt gagatcacct gttataacgg tatgactaca
     2401 ataaaatcgg aactgaccag ccggacagat tccggagatg tcagagggat catccggaac
     2461 gtcatggaag agaataaaag tcccaagctt ccgggacagc cttcctttac cggaggtctg
     2521 gtaggatatt tttcctatga ctatatcaaa tacagcgaac cgaccctgcg actggatgcg
     2581 aaggacgagg aaggcttcca ggatgtgaac ctgatgctgt tccacaaggt catcgctttt
     2641 gacaactaca gacagaagct gatcctgatc ctgaatatca agaccgatga tctggagatc
     2701 aattatcata gagcagagcg tgaactgaac aacatgaagg agcttttgct gaatggggaa
     2761 aaggcagagc atattccctg taagctccgg acggattttc aaccactgtt caatgaagca
     2821 gcctattgca gcatggtcga gaaagccaag cattacatca gagagggaga tatcttccag
     2881 gtggtattat ccaatcgcct ggaggcggag atggaaggaa gcctcttaga tgcttaccgg
     2941 gtactccgca caacaaatcc ttctccgtac atgttctatt tctccggcag cgatggcgag
     3001 attgttggag cgagcccgga gacattggtg aaactggaag atggtgtatt acataccttc
     3061 cccctggcag gaacgagacc cagaggagct accgaggaag cggacaaagc actggaagca
     3121 gaacttctgg cagacgaaaa ggaacgttcc gaacacaata tgctggtgga tctgggacga
     3181 aacgatatcg gcaagatcag ccggatcggc accgtggaag tggaaaagta tatgtccatc
     3241 gagcgttact ctcacgtgat gcacatcgga tctacggtaa gaggtgtgat cagagaggat
     3301 gcggatgctt tggatgcggt ggatgccatc ctccccgcag gaaccctgtc gggagcaccg
     3361 aagcttcggg cctgtgagat catcaacgaa ctggaagaca ataagagagg catctatgga
     3421 ggagccattg gctatatctc ctttaccgga aacctggata cctgtatcgc tataagactt
     3481 gctttttcca agaacggaaa agtattcgta agatccggtg ccggtattgt ggcggacagt
     3541 gtaccggaga aggaatacag ggaatgcatt cagaaggcag cagctgtaag acaggcacta
     3601 cagaaagcac aggagggaat ggaagcatga tattactgat cgacaattat gacagttttt
     3661 cctataacgt atatcagctg gtaggaagcg taaatccgga tattcgggtg atccgcaatg
     3721 atgaatgcag tgtggaagag atccgggcca tgaatccttc ccatattatc ttatcgccgg
     3781 ggcccggaag gccggacaaa gccggtgtct gtgaagaagt gattcgggca ctgggcggaa
     3841 aaatccccat tcttggtatc tgcctgggac atcaggccat ctgcgaggtg gcgggagcca
     3901 ctgtggctta tgcatcccat ctgatgcacg gtaaacagtc cctggcgact ttggatacag
     3961 acagtgtatt gttccggggt atgaaaaaag tgatcactgt ggcaagatat cattctctgg
     4021 tggcagatcc acagacgatt cccgcagaat tgaaagttac cgcggtcacc gaagacggag
     4081 aggtcatggc ggtagaacag acagaaaagc agatctatgg agtacagttc catccggaat
     4141 cggtactgac accggatggt cgacagacca ttgttaattt tttacagaca cagaaaggag
     4201 caggcagaaa catgattaaa gaagcggttg ctaagttggt aaagaatgag gacatcggat
     4261 acgatatggc aaaaaccgtt atggatgaga tcatgagcgg tgaggccagc gatattttaa
     4321 agagtgctta tctgacagca ttaagccaga agggtgagac catcgaagag atcacaggct
     4381 ccgcagaaga aatgcgaaag ttcggcagaa aacttggagc ggacgtggaa gctttagaga
     4441 ttgtgggaac cggcggtgac ggatctaatt ctttcaatat ctccactacg gcatctatcg
     4501 tgatctctgc ggcaggcgta ccggtagcca agcacggcaa ccgcgcagcc agttccaaat
     4561 ccggtgcagc agactgcctg gaagctctgg gcgtaaatat caccatagag ccggaacaga
     4621 gcaagacgtt gttggaagag atcggcatct gcttcctttt cgcacagaaa tatcacaccg
     4681 ccatgaaata tgtgggaccc atccgcaagg agttgggtat ccgtacgatc ttcaacattt
     4741 taggaccgct ggcaaatccg gcaggaccgg tatatcagat catgggtgtg tacgatgaaa
     4801 gcctgctgcc gtccatggca aaggtattgt ccaatctggg agtaaagaga ggcatggtag
     4861 tctacgggca ggacagactg gacgagatct ccgccagtgc acccactaca gtctgcgaga
     4921 ttgataacgg tacctttaaa aattatgtga tcacaccgga aaaatttggc atggtaagat
     4981 gcacgaagga agatctggtg ggcggaactc cccaggagaa cgcagagatc accagagcta
     5041 tcttaaacgg agaaaaaggt cccaggcgca atgcggtact gctgaatgca gcagcagccc
     5101 tatatgtggc aggcaaggca gacagtatgg cagacggtgt gaagctggca gagaaggtca
     5161 ttgataccgg ccttgcaaag gcacagctcg agcgttttat caagctgagc aatgcatgat
     5221 aaagtgttac gagtactttt gacctgcggc gcaagtatta cgattatgaa acggaaaggc
     5281 aggatgaatg gatggtactg gatactatag cagcctctgc aaaggagcgg gtggcagcag
     5341 ccaaagaagc actgcccctt acagaacaga tcgccagagc aagagatttg gacagcaaca
     5401 cgggatttcc ctttgaacag gcactggcga agcaggggat gagctttatc tgtgaggtga
     5461 aaaaagcatc gccctccaag ggactgatcg cagcggaatt tccctatgtg cagattgcga
     5521 gggaatacga agcagcagga gcggatgcaa tttccgtact gacggaaccg gcgtactttc
     5581 aagggaagaa cgaatatctg accgagatcc gtcaggcagt gaagattcca ctgcttcgga
     5641 aagattttac ggtagatgaa tacatgatct atgaggcaaa aaatatcgga gcaaatgcag
     5701 tgcttttaat ctgtgccatc ctgtcaccga tgcagttgtc agagtatgcg ggaatcgcag
     5761 gagaacttgg actgtcggca ctggtggaag cccatgatga gaaggaagta gagatggcat
     5821 tgaaagccgg tgccaggatc gtcggtgtca ataaccggaa tctgaaagac tttacggtgg
     5881 atatcaataa ctccgtgcgg ttgagggaaa tggtgccgga gaatattctc ttcgtatccg
     5941 aaagtggtat gaagacgaga caggatatcg agcggttgga gcagaacggt accaatgccg
     6001 tccttatcgg agagactctg atgagaagtg cggataagaa agcggtattg caggagcttc
     6061 gcggacagtg tgaaaaataa atttttcata cgcaaatttg tgcctacggc ccaaagtttg
     6121 ccggtcatag aaaggttgta aatggtatga aagtaaaaat gtgtggtctc tatcgtccgg
     6181 aggatattgc ttatgccaat gaggtgcagc cggactatgt gggatttgta ttttacaaaa
     6241 agagtcaccg ggcggttacg aaggagcagg cagccaaata tcgcaaaaag cttctgccgg
     6301 ggattcggac ggtgggtgtg tttgtaaatg aggatccgaa gaacattata gaactgctgg
     6361 aggaagatat tttggatgtg gcacagcttc acggggatga gacagaggag gatatccagt
     6421 atcttcaggc ggtctgcggt aaaccggtga tcaaagcagt taaagtcaga gaccggtatg
     6481 atgtggaagc ctggctggac agcagtgcag actatctgtt attcgataac ggaatgggga
     6541 cgggacaggc gtttgactgg agcgtccttg gcgggatcga cagggaattt ttcctggcag
     6601 gaggattaca ggcggagaat ctcaccgaag ccatggaaga ggtaagacct tacgcggtag
     6661 atattagctc cggcatcgag agcgaccgga aaaaagatcc agagaagatg catcggatca
     6721 tggaattggt agagcagtat cggaacaatc cgcaggcatc aacatcaaag tcgggggctt
     6781 ttgagcccga catcaatgta tagaagaatt ataaatcaga aatcaaaatg aaaatcagga
     6841 ataaaaatca ggatgaaaga aaagaggagg aacacatgag caaaggacgt tacggaatcc
     6901 acggaggaca atatatctcc gagacattga tgaatgaact gatccatctg gaggagtgct
     6961 atgagcatta taaaaaggat ccggaattca atgcggaact aaataagctg ttgaatgaat
     7021 atgccggtcg cccctctttg ctgtattatg cggagaagat gaccaaggat ctgggcggcg
     7081 cgaagatcta tttaaagaga gaggacttaa atcacactgg ttcccacaag atcaacaatg
     7141 ccctgggaca ggcgttgctt gccaagaaga tgggtaagac ccgtctgatc gcagagaccg
     7201 gtgccggaca gcatggtgtg gctaccgcta cggcagcagc ctttttaggg atggaatgtg
     7261 agatcttcat gggaaaagaa gataccgaca gacaggcgct gaatgtttat cgtatggagc
     7321 ttttgggagc aaaggtgcat cctgtgactt ccggtaccat gacattaaag gatgccgtca
     7381 atgagaccat gagagaatgg tccaaccgcg tggcggacac ccattatgta ctgggatccg
     7441 ttatgggacc gcatcctttc cccatgatcg taagagattt tcagagtgtc atcagtcagg
     7501 aagcaaaaga gcagatcctg aaggcagaag gaaagcttcc ggcagccgtg gtagcctgtg
     7561 tgggcggcgg cagcaatgcc atgggtgcat tttacaattt tattgaggat caggatgtag
     7621 aactcatcgg ctgtgaggca gcaggcaaag gtgtggatac ggcactgacc gcagctacga
     7681 ttgcgacggg atccttagga atcttccatg gcatgaaatc ctatttctgc caggatgagt
     7741 atggacagat cgctcccgta tattccattt ccgcaggact ggattatccc ggcatcggac
     7801 cggagcatgc ttatctgcat gacattggca gagcacagta tgtgcccatc acggatgatg
     7861 aagcagtgga tgcgtttgaa tatctggcaa gaaccgaggg aatcatctgc gccattgaga
     7921 gtgcccatgc ggtagcacag gtgcgtaaga tcgcaaagaa ttacagcccg gatcagagca
     7981 ttatcgtatg tctgtccggc agaggtgaca aggacgtggc agccatcgca agataccgtg
     8041 gcgtggatat tcatgactga tcgttttgac ggtaaagcca tcataggagt aacagacaga
     8101 agaaagtaaa aatcaaaaca gaaattacaa aataagaaga tggagataac aacaatgaat
     8161 aattatttgg ataaagtatt tgatactccc aataaaaaag cattcatagc tttccttacc
     8221 gcaggagacc cggatgcgga cagcactgta aagtttattc tggagatgga gaaggcaggg
     8281 gcggatctca tagagatcgg tattcccttt tctgatccca cggcggaagg tgtggtgatc
     8341 caggaggcaa atatccgttc tcttagcaac ggtatgacca cggacggtgt gtttgagatc
     8401 gtgaagagag tgcgggagca gtcggagatc cccctggcgt ttatgactta tgtaaatccc
     8461 gtattccatt atgggtatga gaaatttttt acaaagtgtg aggaactgga catcagcgct
     8521 atcattattc ccgatgtacc gtatgaagag aaggcagagg tggaggctcc cgctaaagcc
     8581 catggtgtga aggtgatctc catgatcgca cccacttccg agagccgtat ccgccagatt
     8641 gcatcggagg cggaaggatt catctatgtg gtaagctcca tgggtgtcac cggtgtgcgc
     8701 agtgagatca agaccgatct gcctgctatc gtggaaagca tccgcaccgt gaccgacgtt
     8761 ccctgtgcta tcggtttcgg catcagcact cccgagcagg ccaaggctat ggcatacata
     8821 tccgacggag ccattgtcgg aagtgccatt gtgaagatca ttgcaaagta tggtaaagac
     8881 gcagctccct atatcggaga atatgtgaag agcatgaaag aggccgtagc agagtagaca
     8941 acgataggag gtatgacacg ataatctagg aaacggttat tgctgtaact acttgagcaa
     9001 aaggaaatta ataagattaa gatggagagt ttccaaatgc tcaattgatt tagacgtatg
     9061 attgtggtaa atgtgaattg tcaagtatgt tcatttttgc ttcattgctt tttagataac
     9121 ttaatcgtat aattgtatca ttgctagtac aattaaaggg gtaaagttat gcaaaaggaa
     9181 gcatttggta aactactgat aattttaagg aagaagaatg gattaagtca gaaggaactg
     9241 gcagagaggt tgtcgatttc tacgtctgcg gtaagcaaat gggagaatgg aaagaacctt
     9301 ccggatatga tgatgcttag tagtatagca gagatgcttc aggtatcgtg tgacgaatta
     9361 cataatccgg aaaagacatt ggagaagctg aacaatcctg aatttcaaaa aaggaatgtg
     9421 gaggagggag ataaggagaa tacggataac acaggggaaa atgaggaatc agaacctgaa
     9481 ccccaaaaga aaaactatgg acgaattgta agaatgtcct ttttgattgg aatattggcg
     9541 atagttgcag gaatgtttct gatatatatg gtaggacata gaaaacctgt ttttcagcag
     9601 ataggaacaa ggtatattga tgatccgtta tggggaaggg tatatgaaat tgcgtatgta
     9661 gtaaatggtg aaatgacgaa tgatagtatt aatgtgcatt tagatgaggt gagaaataca
     9721 ttggatcaag aagtaatagg taccaatatt gtaaagacag cttattatga cagtaagaaa
     9781 gatgctacag catggaagga aacagatgta ggaggatatg tatttctgag tggagaatga
     9841 acggtgagga tacgacaacg gtttatgcaa gaaatcatgc agtacgcccc atttcaggaa
     9901 gcgatgtaat ctcattaaaa gaatctgatt caactaatac aagtgcagcc gcatatgcaa
     9961 ctgtttctta cgacggaaga taagttatat gagggaaatt gtatgttaat aaaagctatt
    10021 aagaaccaaa ccattttttt attaaaacaa aaagaagcaa gtctgacatt tatattactt
    10081 c
//
LOCUS       DAWDHD010000085         9841 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_412_length_9841_cov_12.363376, whole genome shotgun sequence.
ACCESSION   DAWDHD010000085 DAWDHD010000000
VERSION     DAWDHD010000085.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 9841)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9841)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9841
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_412_length_9841_cov_12.363376"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2268 a         2458 c         2070 g         3045 t
ORIGIN      
        1 acggaagcag caagaatagg aagtgccgca ttcttggcac ctcctatttc aacttcaccg
       61 actaatggat taccgccttt aattgcgtat tgttccattg tttcttacct ctgtcattct
      121 cagatcagaa aaaccttctt atgcacttat accacatttt cgcacatttg taaatacaac
      181 gccctaattc aggtacttca acgggttcac ctggctgcca ttaatgagga tctcaaagtg
      241 aagatgaggt cccgtactta cgccggtatt accgctcagt gcaatacgtt ctccctgttt
      301 taccgtctgg ccgaccttga cgagcacctt gctcagatgt ccgtatcggg tctgtctgcc
      361 gtcttcatgg ttgatataga cacaataacc gtatccactt ccccatccgg ctctggccac
      421 tgtaccgccg caggatgcaa ataccgcagt tccggtagga accgaccagt ccaccccctt
      481 atggtagctg ctggcaccct tggtaggtga ctttctgcgt ccgaaattgg aactcagacg
      541 tccaccggaa ataggcttga tataggtcgg aggaatcttg gtgcctcgtt ccacaatctt
      601 gggaactgcc tccatgacca cttcttcctt cagaatatcc ctggtgacct ctctgtcatt
      661 cagataagat acatccgcca ctacccgacg gaaaccagcg gagggctgct gcaaaatctt
      721 ggtctgggtc gtataccagg aatcgttgtc cacataaatt acatctgcat catagatttc
      781 ttcataataa ttttgctcca tgcgctccac ggacagctcc ggctgcggca cggtaatgat
      841 cagcttgtcc cctacgcgga tcgtagaatt ttcatcggtc agtgtctcgc tgttcatggc
      901 gatgatctga tccatcggaa tatttacctt aatcgaaatc tccgacaggg tatctccgct
      961 gactacttca tattctccgg gggtctcctg atccttgatc acatcctcga ttgcctgatc
     1021 cagcggagtc agcatgcttt ccggaagata ggtctctacg atttccacct tttccgcaaa
     1081 gtccatggtc atcagcccaa gatcatagtc ttcgaaatct ttttccccct gctggtctgc
     1141 ctgggagccc gcctgatcca gtacggactg tacaccgcct gcatagatgg attcttcctt
     1201 ctgttcttcc tgagcggtca gttcctgttt attgactacc tcggtggtaa gaacattgaa
     1261 ttccttctgt gcatcgtgag tcagttccac gctgaatttg tcttcactgt cgtatttgtc
     1321 cactgctgcc tgcagcagag actccacatc ctccacgctg gacaggttga ccatatattc
     1381 atttactttc attgtatagg atctgtggga agtctcacga atactgctct ccagttcccg
     1441 ttccatggcc gccagcagat tgtcctcatt gtccagtgtt ccccacagca cttcttcacc
     1501 gaccagcttt aaatctgcgt ccataaagat catatcatca ctctgggaag cgatattcct
     1561 tctggcttcc tgcagaagct gctccgctct gtctgcttcg ctcaccgttc cgatctcatt
     1621 gccgttcaag aacacgtgga agtaattctc tccggtcttt tcaaacgctg catacccttt
     1681 gaggaaaaag aggctcacaa aaagagtggc cagcgttgtt ttcacatatg tatattttac
     1741 tcttttcgag taagatgtca gtttcttcat gaattccttc tcgatctatc gtatcagaaa
     1801 acagtatgcc cccttcgggc attctgtata cctgtcttac tgtaataact ttgtaacata
     1861 tagattctat cagcatttat atgccttgta aagaaagaat gtctgttcga tttttttgtt
     1921 ttctcccgaa ccgatctatg ctatactgga ggtaaataaa acggtctgat ccggaaagga
     1981 gacgccatga gggaaccggt catttttcat attgatgtca attccgcttt tttaagctgg
     2041 acggcgctgg cccgtctgga acagggggat cctgtggatc tgcgcacgat cccttccatc
     2101 gtaggcggtg atacctccac cagacacggt gtggtgctgg ccaaatccat tcccgccaag
     2161 gctttcggca tcactaccgg tgaaccggtg gtcaatgccc tgcgcaaatg tccgcatctg
     2221 gtcatggctt ctccggatca taatatgtac cgtctgaaaa gccagatgct tatggaacat
     2281 ctggcgggta tctgccccga catcgaacag gtcagcatcg atgaatgtta tatggactat
     2341 acgcccattg cggcgaacta cgcctctccc gaggaagccg ctgctttcat tcgtcactct
     2401 gtttttgaaa aatttaaatt tacggtaaat gtgggaattt cggatcgaaa ggttctggcc
     2461 aagatggcct ctgattttga gaaaccggat aagactcaca ccttgtatca gcgggaaata
     2521 gcgcagaaaa tgtggccgct gcctgtttcc gccctgttca cctgcggaca ttccagtgcg
     2581 gagactctcc acaaattagg tattctcacg atcggggatc tggcaaagac ggatcctgcg
     2641 atcctggaag cacatttaaa aagtcacggt cgttgtctgt ggcagtatgc taacggcatc
     2701 gacgattcca cagtagtcct ggctccttcc aaggcaaaag ggatcggcaa ctccaccact
     2761 ttaagcagcg atgccaccac caaagaggaa gcctgtaaag tattgctgtc tctgtgtgaa
     2821 tccgtgggca ggcgtctacg ggcctcccac cagttggcag gacagatctg cacggagatc
     2881 cggtacagtg atttccggaa cgtttctcac caaatgccga tagacattcc cacggcttcc
     2941 gtgaatctcc tgtacgaaga tgcctgtgca ttattcgacg aattatggga ttccactccc
     3001 atacggcttc tggggatccg cacttccaag ctggtgccgg aagacgaacc ggttcagctc
     3061 agcctgtttg attttccggc tccccgttcc gagaaacagc agaagctgga tgccgcactg
     3121 gataatatcc gggcaaaata tggcaaggat gccatcaagc gggggcgttt tctctaacgc
     3181 tgcgtggtaa tgatcatgtt gagtaccgcc ttggtctcac ccacattgtg atagacatgt
     3241 ttcctgtcac aggcaaaata gagagaacca cctgtcttca ccagaaaact ggaatcttcc
     3301 agaatcagtt cgacctcccc ggataccacc gtcaggtatt cccaggtctg ctccccgtgg
     3361 gattcgcttt ccatccgggc atttctggtc agctcgccct gatagatctc gaaatttctt
     3421 ccggcatcgt agggcagcat cacatagatc cggtattccc cttcctttgc cttccatacc
     3481 ggagcctctt ccggagacgg caccaccatg atttctttcc tattataaat caactgttca
     3541 aagggtacct tcaatccctc cacgatcttc ccgatagtgg acaccgtggg attggattct
     3601 cctctctcga tctgtcccag catgcttttg ctgacaccgg tacgctccgc cagcatatcc
     3661 agactcatat ttttggattt gcggattctt ttcagattga atgctatgtt tttatcgatc
     3721 tgtgtcatat gcttcacccg gactttccca tgattgcggt acccggcaga tattttcccg
     3781 ccaccgcata aaaaaagcat cagactcccg tctgatgcta cttcttcgta gtaaaattta
     3841 ccatgttctt atttatgact tatgataccg aaacggaagg caaacgtcaa gtaatttttt
     3901 acctgtgtta ctcatcgctt ctttacacta tatccgaagg ttcccagcaa ttcgccacag
     3961 ccgaaatagg aaccgtggga atagatgatg ggttccgcct gctccaggtg gaatttgtgc
     4021 ttctcatcca tatatttttc atcggcgtgt actgccacca catccgccag gaacatatca
     4081 tgggatccca gcggcaggat ctgtcgcacc ttacattcaa tgttcaccgg actctctttt
     4141 atcatcggtg ctttcacctc agatgccgga atgggagtca gtcccatttc tttgaattta
     4201 tccacgtccc gtccgctttt cacgccacag taatctgtgg caaatgccag ctccttcggg
     4261 ggcatattga tctcgaattc tcctgtctcc ttcaggatcg gataggaata tctctcgggg
     4321 cgtacggaga tcgacaccat gggcggattg gtacatacgg ttcctaccca ggccagcgtg
     4381 ataatattgg ggcggccgtc ccggtcagcc aggctcacca tgaccaccgg caggggatac
     4441 agcatatttc ccggcttcca aagttgtttt cccatttccg taaaacctca tttccgttac
     4501 tttcttatca atttcagcat tcttaaatga atactttatc tgccttttca gaaaatgtgc
     4561 aaaatgctca atacatgaag aacaatatcc gcaaccgtca gaactactgc cgcaatggat
     4621 actcccagca caccgttcaa tttaccgcca aggttttcct ttacttttgc tgcgatggct
     4681 tcggtctgct tctcgttctc ttctaccaca accgcctgta cattacggta tacctttacg
     4741 cattccttat gtacattctc ttccgtagtg ctcagtttct catccacatg cttctgaagc
     4801 tcgtccaaca cctcggtact ctgctgcagt tcttccagtt tggcagtata ctgctgtccc
     4861 agagcttcca gtttctctgt gttctcctgc tgcagcttct gcagggcagc cacgccttcc
     4921 tcggaaagct tcgtcacttc cggagcaatg gtctcggaca tcagcttctc catctcctga
     4981 ttgatcgctt tcagttcctt attgacctgc tgcatctgtg ccaggcactc gtcgtattcc
     5041 cgcagctggc ttttcagctg atccatctct tcggcatcag ccgcggaatt cgccttgatc
     5101 atttcctggg cattcagctt ttgtgcaagt ttatccataa aagtatccat ccatcttcct
     5161 ccaaacatat ttcgtccctc atgtgaacag tttccctttt caactgtccc tattttacca
     5221 tgtttattcc ccggttacaa ccaaaacccc atgaatactg tgttttcatg gtttgcgcat
     5281 ttttatgcaa tttgtacagt ttcttttttg gtttctgtac tttttcgtaa aattcaccaa
     5341 gttttcacat cgtgttcata atttgttcat aattggttgg tatactttaa atacagtcaa
     5401 ggaagagata agacttccgg ataacaaagc atataagata aattttttca taatagccca
     5461 ggcgccgaaa ggtgtctggg cactcctcat tttatgggga ttttcctgtc atcccattac
     5521 tgttttgacc ataaaaaaga atctcgcttg cgagattctc cgcctgcggc gggtcgcttt
     5581 tgcgacatat ttcataaccg ccgcagtgcg atccctgcgg agcattttta attcatagga
     5641 agaaatttcc tatgaattaa aaatgcttct gtcgtttgta ttacaccgac agaagctttt
     5701 ggattatttg gatgttacgc tatttaattg tttttcctct tctctctttt ggctgatggc
     5761 tagtatctct tcgttcagag acagcatcct cgcatccaga tcagacacca ttctcgcaca
     5821 ctccaccagt tcatctttaa tgctctccgg agcatcgccc tcgaatttgt aatggatctt
     5881 atgctccagg ctggcccaga agtccatggc aaccgtccgg atctgtatct ccaccttcgt
     5941 atccaccgtg cggtccgaca gatagaccgg aatactgacg atcatatgat agcttttata
     6001 gccactggct ttgggatacg taatgtaatc cttcaccgcg atcacctgaa tatccttctg
     6061 atcccggatc atatccgcaa tgcggtaaat gtccgatgta aaggaacaga tgatgcggat
     6121 gccagcaata tcgtttatgt attttaccat gttatctatg gtggattcct gcccgtagcg
     6181 tttgagcttt ttcacaatgc tttccggggt cttgatccgt gccttgatgt gttcgatggg
     6241 attgtactgg tgtacatgct gaaactcgtc gttcagaatc tgcatctttg tctccatctg
     6301 acgcagtgcg gcgctgtata ccagcgtgac ctccttccag ctgtcgattc catccccgtt
     6361 ttctacctta aactccatat tacctctcaa tctcttctgt cacagaaggc tctgcgacca
     6421 tttttcatat ttgttacttc cgtagacgct ttttcgttac ggaaattctg cattcgtacg
     6481 ttgatattta tgataattat agcatacctc cttttaaaaa tgtataattt taacaaaacc
     6541 tttattattt tttaagattg attgggcagt tttatagtac gctttcgcaa acgtgccgat
     6601 tcggcggacg aaagctatcc catatacaat ataagatgga tctgatggtt gtgtgacttt
     6661 ttttgcttgc aggggaaacc tcttcctatt ataatttatt attaacgacc aatatgtctg
     6721 attttttgcg taaggagatt ttctatgttc cgtatgtggg ccaaaatttt taaaaataat
     6781 catatgctga aagacacctg catcgaggac ggcagtgacg atacccgtac ccataaagtt
     6841 tttcactctc tggacgaagt ctgccatcag tttgatctcg gtacgcccat ctggctggat
     6901 atgaatattg cagactttaa acgtcacaac aagacccgtt ttacccagga cagttttgta
     6961 gagtccatcg attttgacta tctggagatc cagatcattg aggaggatta agatgaacag
     7021 caattattcc gcacaagaga aaaattttgc aaccgcattg ctgcataatc tgtggaaata
     7081 tctgttgctt gccgcatcta tagttgctac cgtcaagatt attttcttcg gattcgacat
     7141 tgacgagcag tatgccgtat ctatggctta ccgtctggta cagggagaca gaatgtttct
     7201 ggaaatgtgg gagcctcatc agacttccgc attcttttct gctacctttt tatggctgta
     7261 tatgcagttg tttcacacct taaaatactc cgtctctttc tgcgaatcgt aggtgtcgtt
     7321 acacagttgc tcatcagtat tctgacctac cggactttcc gtaagtttgt aactgagaat
     7381 actgcttttg tcattgctgt tttttactac aatatcattt ccaagaacag taccgttccc
     7441 gatttttcca atatgcttct gtggttcagt atgcttctgt ttctctgcct cctgtacttt
     7501 tttcttgcgg agaacaatga gatacctgga aaatatttct gtcttattat gagtgggctg
     7561 tctacttcgg cactcgttct ttcctatccc acctgcctgt ttgtcgtagt gcctgtcagc
     7621 ataggaatct gctgtgtttc aacctttaaa aacagatggt gtaatcttat gtgttacttg
     7681 ttcacctgtg ccttttgtgg tattagttgg ttgtcttatt ttctttttca tatgtccttt
     7741 tctcgttttg tcgcaggtgt atccgccatg ttctccgacg gttctcactc tgatacattt
     7801 gcaagcaaat tgagaggcct ttttagctat atttatgata ttcttcccct atttcttgct
     7861 gcggctcttt gttcatttgt tttatggaaa tttttaaatg ttatttcaaa gaaacgatat
     7921 gcctattctc ttatcttaat tatattgctt gcagtggaac agattttggt ttggtacttt
     7981 atgcaaaaac ggttgaatta tcccggactc ctttcctact ttttgctttt tcttgctttc
     8041 cggtattatc atctgcatag gtcagaagtg aaagacagaa ctactatatc ttatgccctt
     8101 ttctggtttg gaagcattgc ttcgctgttt cttctggcgg ctggtttcct tgccagtaac
     8161 acacaattgt atgagtccat tggttacgtt actattggca tcgttgtttt tctgtatttt
     8221 cttgacaaag agcaaaagag aaaccgctgt ctttggaaag ttgtgatctt tgccattata
     8281 ggaactgcca ttcttcacaa aggtttctac gtgtcctatc tatatggaca tgatacgata
     8341 tttgtttccc ggcaaaaggc tgaagacggt cccatggctg gaatctacgg ccgctattct
     8401 gatggacacg aatataatct tcggaatcag ttgctacaca cgtatatccc agaaagaagt
     8461 aatcttttac tagtcagtaa caaaactatt atgtacttgc aaggcgatta cgtgatctgc
     8521 aactattcca ccatcagcac tccgaccatc gatgagcgct tattccaata ctggacgctc
     8581 tatccggaca aagtgcctga atatattgta tgggataaag gttctgaagg ctacattgca
     8641 acaaatccgg aagtaaatgc ccgtctggta gagaatgcgg agttactggt tgatgatgaa
     8701 gggctgttga tctacagact tcctgccaat ccttattgat acttttctcc caaatatctg
     8761 aatgactatt gacttttcct tttttgtgca gtagtatttg cattgtaaga tgtagttttg
     8821 aaaactacat ataaaagtat ttcagaatac gtgttgttat tttgaatagt aaatactatt
     8881 gtcggataaa atgatcttaa atagattcga tttgcagacg gcagctgctg tcgggaaaag
     8941 gaggagttat tatgcagatt accataagag agccgggaag tgccattacc catttcattg
     9001 ccatgatgat ggcagtgttc gcagctgtgc cactgttagt gaaagccggt gtacagtccg
     9061 ggcaggagaa tttcctggcc atggccattt tcatgggcag catgatcctg ttatacggtg
     9121 ccagcgccac ttatcattcc gtggacctca ccggtaaaag tctccgggtc ttccggaagc
     9181 tggatcatat gatgatcttt gtattgatcg ccggttctta cacaccggtg tgtctcattg
     9241 tgttgggcgg taagctggga tatacactgc tggctcttgt ctggggcatt gccgctgtcg
     9301 gcatgatcgt aaaagcctgc tggatcacct gtcccaagtg gttttcctct gtgatctaca
     9361 ttgccatggg ctgggtatgt gtcctggtat tcgggcctct gctgaaaact ctgtccacgc
     9421 ccgcttttct ctggctgttg gccggcggca tcatctacac cgtgggcggt gtcatctatg
     9481 ccctgaagct gccgatcttc aatgcgaaac ataagttctt cggttcccac gaagtgttcc
     9541 acctcttcgt catgggcgga agcgtatgtc atttcatttt tatgtattta tatgtagcat
     9601 agctacgagc catgcaaaaa tagagtggaa cacctcgatg ggcgcttgcg cacaagcgag
     9661 gctggttcac tctattttta taccgtgcgt cccgccttat cggacgcact tgacagccgt
     9721 ctatgaaaat aagattgaca gcgttccttg ctgacaatct tatgaatggc acggggcatt
     9781 gcggagggta gtttcaaatc cacgaacgtc cacagcaaac ggaacatcct tccgcggtgg
     9841 g
//
LOCUS       DAWDHD010000086         9792 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_416_length_9792_cov_9.433193, whole genome shotgun sequence.
ACCESSION   DAWDHD010000086 DAWDHD010000000
VERSION     DAWDHD010000086.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 9792)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9792)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9792
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_416_length_9792_cov_9.433193"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2391 a         2741 c         1911 g         2749 t
ORIGIN      
        1 cgcatcttcc ggctcacagt gggaatggaa tctttcaaag tagattccat aactgcgata
       61 ttttaataac atctctttac aatccggaat tgtcatgggt ggatgcccgg tctccgggta
      121 ttctgcgcaa tttgcctcac cccgcagaaa aattgccctg ccgttcagtg ccagtctgcc
      181 gctgccgtta tcaccaaaag agcggatgcc gaaacgggtc ctgcactcag cagtgctacc
      241 gcctttatct tctgctgaca taccgttgtc cagggttaca gccatctcat agagattgcc
      301 ctcatcttcg tcccacagtt tcacattctc ccgcagaggc agatcacgga accagatttc
      361 cattgttttc ggattctcct ctttatctgt tccggcttcc gctaatccct ccccggaata
      421 gctgataatt tccgtcaatg tctgtgtgtc ttctaactct cctgccgcca gagcctctga
      481 ctgtaatatg attttcgcat ctttatatac tttctttgcg ccaggtgcca gttccacaca
      541 tacatccagc acatatccgc ctgctttatt tttctcttcc tttttcaccg ctctcggata
      601 tacccggagg ctgtctataa aattctgcgg tctggtatac atgctgcatt cgccaagtat
      661 cccgttccag tttgtctgtg tctcatccgt cgctgcggaa gaataacaga tggcagcttt
      721 gggcattccc ggatagctgt tatccgaaag aaacgtaaat tcatgttctc ccggtgccgc
      781 cccggtgagt tcaaaaatat aaggtgtact tagtgtcccc tgccggaaca ccgcacacgc
      841 ttctccatcc actagcagcc ggagtgcccg tgcccgctcc gccagtacga acagcctgtc
      901 ggttccgtaa tccggtaccg ttatcttacg actgatcctg gcttctccct cataggtatg
      961 ccgcctggta aatctcgtag cgatcggtgc atctttgtct atctctcccg ccgcattccc
     1021 cagtaccgca tccggatgcc actgattcgc ccccacatct ctatgtccga tcccgttctc
     1081 atccaacgtc cccggcagat ccatctgtcc cgtggttccg tcctccagta ctacatgcca
     1141 ttttccattc aacaatattc tcatataagc tccatcccac agaccctttt ccatacagaa
     1201 aatcccctag cagatccata gtaaattttc tgatgttttc tccttgatca cggaatcatt
     1261 atatagctta actttagtgt tttaaattca ctatcatgaa ctatttttta tattcattgc
     1321 attattctga acctttttga tataatatga tgccaggggc gaaaggtcta agccaataag
     1381 cacggaacaa tccgtaggca tcaaagttaa gggcttttga ccccgacatc ataatacctg
     1441 agaggagtga atcagaaatg acagatagaa gaactttttt atgcgaagat tttgaaattt
     1501 cctatgaaga tacaaaaaaa ggatggtcca tgccctattc gcatcaccac accaactatg
     1561 aaatctatat tctgctctcc ggggagcgtc tggttaccat aggcgatatc acctaccggg
     1621 taaaagccgg catggcaagc ctgtttgaga gcaatatctc tcaccgaagc gtcggtgaca
     1681 cggattatac cgggatctgc atccattttt caaaagtcta tcttgaaaaa tactttttac
     1741 ctgccgtagt atcttcctac ctgacctgtt tcaaggcacc gattattttc attcccgagg
     1801 attacagaca aaagctcgtc gactggaacg cagcttcctg ctgcaaatct cccactgcct
     1861 acctgttgct tgcacagatt cttacagatc tggcagcttt tcgctatcag tatcctcttg
     1921 aaaatgaaat ttctgtcagt gagaataaag cttccggttt tcagcggatc attaattata
     1981 ttaacgcaaa ctatacaacg cttcagaccg tccgggatat cgccgaatcc tgcggtgtgt
     2041 ccgaaagcta tgtccaccgg gctgtccggc ggacagaatc cctcaccgta aaagaatata
     2101 ttaataagct tcgcctccgt catgccatac accaggtgga acagggcggc aattctctct
     2161 ctgtcatcag caagtcctgt ggtttccaaa gcgtttctta tttttaccgg gtttttaaaa
     2221 aacactacgg catgacccct acacaatatt tttccaaaaa agaaccccat tactttctgc
     2281 agatcactta gcatcctgat ggatgcctta atggcaagcc ttcaggaaat cctaaagctc
     2341 agcaatgagc agttggctgt atttactgct gattttgaat caagactacc gagatatctg
     2401 cggaatgcac ttcatccgga agcagtagcg gcataaacca ctattttgtt agctgaaatt
     2461 ttctttttgt taacattatc tctttttata aatcagaagc tccggatttt caccattctt
     2521 ctcatatgta cataccagaa taaaatccat acctgccaga ataccgaagc atctgtctcc
     2581 tccgaattca gactccagtt ggttccccag ataggttttc tgatcatcaa ggaatttcat
     2641 tttctgtcca tttggcagac tatattccag attcacatgt ttccccacaa gtgcatttag
     2701 tttgtctacc cttgggagtc catctatatg caaatcatta atttcgttca tcaactgctt
     2761 tttaaactct tcaaactgcc cattgtcact aagttcatca taccttttcc agtaatctaa
     2821 ctttggaagc atttctttta aataagagcg tgcctgttct tcggtaaagt tttcatttac
     2881 catattcttt acacacacat caatcaattc atccatatta ttatatatgt atgttccatc
     2941 tgcatagcac catttgcaat actcctcatt taatgtgcca tctttatcac gtcccaatat
     3001 ggaatcatca tcaattggca ttccgcagca ctggcagatc agctttctgg gttctcccaa
     3061 aagcgtattg atggagacat caaattcttt tgataagagc ttaagtgtat cagtattggg
     3121 aacggtatca ccgttttccc aacgcgatac cgcctgcctt gtaaccatga ttttatctgc
     3181 cagctcgtcc tgtgacattc ctctttctgt gcgaagtttc aaaattatat tttttgtttc
     3241 catagtcatt acctccataa ccatatcata tagataaatt catgttatga aaagcaacct
     3301 gctgttgcca tactctatgc ctgtcatcca ccaaaatatt ttcggcactt ttcgctttta
     3361 gtgccaaaag tgtattgaca aaattatgat tatttattac ataatttcaa atctttgtta
     3421 cacgttttca tctccgcaat cttgaaactg cccagcaacg ttccacacac cacttacagt
     3481 ccccgacgtc tcggctgtgc caaccccgac acgacgaaac cggattctcc cgagatcctt
     3541 gccttatagg accgcaaaaa agagggcgag atcgccgaaa actctctggt gatctcaccc
     3601 tctctggatt atcaaccgct acttcatcaa gcctcctcac agattcttcc cgcattccat
     3661 caggtatgca tccattccca gacacattgc accgatgatt ccggggttct cgcccagtgc
     3721 aggagccacc acatactgtg acatatcctt cggcaggctg gtctccggca gatagccatt
     3781 cagaaactcc accgcattct gtctcaccat gtcgaaaacc ttctcctggt gcatcacacc
     3841 gccccagaga atgatcttct ccggtgagta agtcagcatg taatctgcca cagcctgtcc
     3901 gatgtagtaa ctttccagtt cccagacatc ctctcttccg gccagttcag ctccctttct
     3961 gccatatctg ccctgaatcg caggaccgga agccagtcct tccagacagg caccgtgata
     4021 aggacagcag ccttcatagg tatcctccgg gtgcttgcgc aggaagatat gtccgccctc
     4081 gggatgcatc aggccatgca gcagtctacc gttaatatat gcccctacac ctactcccgt
     4141 accaatggtg atatagagcg cattctcaca ccccttggct gcgccgaagc gtacctcgcc
     4201 aagcactgct ccattgacat ctgtatcaaa acctacaggg actttcagtg cttcccggaa
     4261 cgctcccgca aaatcataaa atctccatgc cagcttcgga gtgctggtaa tatagccgta
     4321 ggttgccgat tttttattca gatccaccgg gccgaaacag ccgatcccca gtgcctcgat
     4381 tcctttatcc tgaaaatatg ctatcatttt cggcaccgta ctctccggta tctccgtagg
     4441 gaatactgcc ctgtcgaaaa tatttccctg ctcatctcct atggcacata ccatctttgt
     4501 gccgccggct tcaattccgc ctaatttcat tctaacatcc tcccattaca actttttact
     4561 atgcctgttc cctgttcagg atccgcttat ggaacaccag cacaaacaac agagtaaaga
     4621 gtatcattgc tgccggcatg atcagaaagc tcctgccata tcctgttttt tcagcaatgt
     4681 ctccgtaaat atagttaaat ccaatatcaa acagggatgc tacactgatg atcaatccgg
     4741 acatcgtgga cacctgcgac ggatcataaa gtctcccgat ggccataacc agtgtcggat
     4801 aaatgatcga gcagaacagt ccggccagac tcagcatcca gagtgcccca ctgaagatcc
     4861 ctgccgcata aagcactgca gaaatcacag aaaacaaggc aatgctcggg aataccttca
     4921 gccggtcaac caacggagaa aagaccagtc ttcccaccgt cattccgcca aaaaacaacg
     4981 ccatataatt ggcagccttt ccctgctcca gtcccagcgc attggttccg tagatcacaa
     5041 accagttcag cattccatgt tctgcaataa agtatacacc aaagatcacc accatgacaa
     5101 tccaaaggta gtttcccttc attttccttc cctgctgatc cttttctgcc ttcttcactt
     5161 ctccgctgtc cgggatctgc agttttaaaa tgatcagaaa cgccagaacg ccgatggcaa
     5221 aaagaacccc tgtcacagct ttccagccgc caaaatcgtc agccagattt ccgatcagac
     5281 tctgtcctcc actggtaccg aacccttgca tgaagaacaa tacattgacg atcatggccg
     5341 gtgatgtggc aaaaatgaac ggtgtgatca gtgtaatgga cgtattcaac agcgtagaag
     5401 ctcccattgc caggaacatt cccaccagca atgcataata gttatctgta agcaggaata
     5461 ccagcattgc acacatccat actgcgagaa atcccaaaaa agcctttttg cggtcgatct
     5521 tatccatgat tctgcctccg gccagcagga aacacagatt tcccgcatag cttacggcta
     5581 tgatcgtgga cagccccttt gccgtcaatg caaaatgcgt ctgaaaaatg ccggagaaaa
     5641 tacctctcag gctgtcactg attcccaggc cgaccatcag gattgcaaat ccgatgagag
     5701 cgatccttct attactcttc tccattctgg ctcccatctt ccggctcttc tacatcgatc
     5761 cgtacatttt ctacatttcc cagtgtcacg tcctcctcgt aaaaaggcag gattccttct
     5821 ttcttcgtga tctgaatatc atggaaggag acattcttca cataatccgc atagattccg
     5881 gaaatctttg tgtggatctc tccctctccc tggcagggtc tcttatcgta gccgttgatc
     5941 tcccacttgg atttcttatc cagtgtcagc cgaatgccct caaaggtcac atcttcaata
     6001 taattgtctt cactgcccag caggaaaatg ccgttttctc cctcgcaggt aatattctgg
     6061 aagcggatat tgcggatctt accggcctta actccgggtt tgcgatccaa cgcagtcagg
     6121 cagacagcct ccgctcttcc ccaccattca taagagaatc ttctggtctc cataatgatg
     6181 ttggaaaaga ttacattttc cacgttgcca ttatctctga tctgaatgga aattccacgg
     6241 ttacttctgg aaatacagca attttccacc agaatattcc ggaagtcaga ctctccctcc
     6301 gtaccgaatt tgatggcagc actggtggat accagggtac aattggtgac cacgatattt
     6361 tcacagggac cgtatttctt ataatccccg gaatttttca atacgatggc gtcatctcca
     6421 cattcaatgt gacagttact gatccggaca ttctgacagt gatccggatc gatgccatcg
     6481 gaattcgcca tcttcagatt attcagcagg cggataccgt cgatcagcac atccttacag
     6541 cctaccagat gcactgtcca gaaggcacag ttcgtcaggc ggacttcctt gatggtcaga
     6601 tgctttacat cctccaatag caggagcgga actctgggat aataggaacc ctcgatatga
     6661 taacccgagt catctccgta aaagacagat tcattgccat caatggtacc ctgtccggag
     6721 atcgtcacat tttcctgtcc cagcgcatag ataaaggcat ggaacgggcg tcctgcatat
     6781 tcactgttca ggaaagaagg caggccggat tcatgggctg tgattttacc attgttgcgc
     6841 agcgggtaat aatcttctat gttgctgctg gctttccaga cagcgcccgg ctccagatgt
     6901 acctccacat tggatttcaa cagcacatat ccggaagaat atgtcctacc gccggggaag
     6961 aggactacgc ctcctccctg ttctgcacac gtatcgattg ctttctgaat gacaggggca
     7021 tcattggtct gtccatcgcc cactgcaccg tattctaata tattgatcat aattttctcc
     7081 ttcgtaaccc tctcactttt tttcattcac aatacgcagg gtcttgattt cataaggctt
     7141 catagtaaag ctccatccgt cctgtgcacg ggtcatatcc gcattctcca ccccggaaaa
     7201 attctccatg cagtcacaga ctgttacctg tgcatccgga gcaagtggga agttcacatg
     7261 cactttggta cgcattcccc aagtctcata gatacgtacg atgatgccat tgccgtcctc
     7321 tgctgctttc acagtatctg ccatcacatt ttcctctgct atctgcagca ttgagaagct
     7381 gtcttcatag ataccctgta ccttctccca tgccatcggg cagttcagat cgtaagcttc
     7441 tcttaccaca ctgcccttgc ggaaatctcc ctgatgaggg aacaatgcgt aggtaaattc
     7501 atgctgtccc tgatctgcat cgggattggg gaaaatacct gacttgatca atgtcagtcc
     7561 catcacaccc tcatggacat cataaccgta tttgcagtca ttcatcagtg ccacaccata
     7621 accgggctcg gaaagatctg cccatttgtg tgcgcatacc tcgaatcttg cacgatccca
     7681 ggatgtgttc ttgtgtgtcg gccgctttac attgccgaac tggatctcat aatccgcttc
     7741 atgtgccaga acatccacag ggaaggatac tctgagcagc tgttgatgtt cctgccagtc
     7801 cactctggtc tgaaaatcga tccttgcggt gtgccggtag aaacgaatgg tctgttcgat
     7861 catggaatcc tggaaccgtc tgcgcacatg taatgccgcc gtctccggtc cgttctccaa
     7921 tactttgaat tcttccacat caatgacatc ccaggatttc tccagatatc cctcgtcaat
     7981 attccaggca tcgaattctg ccggccgatc ctcatatacg gtgagacggt tacccatcct
     8041 gcccttttgg atcagttccc ggtcctccct gatatccacc aggcttgtga tctcagcatt
     8101 ggcatcaaat cttacggtaa aatagggcgt tttgaagctt accggtcgtc cgttttcctc
     8161 cattacaagc tcagtgaaac agttgtcctc tccggagttc accgctctgc acacagtaac
     8221 accgctgggt cctgctgtta ttttctgcca actgccctct gcattccgga tcagtgccgt
     8281 acgtgcaaag ctcaaaggat tccatactgc gcagacctcc tcattcttct ccgcacgata
     8341 ccggaacagc ttttcccgga tgctcttctt cgctgttttc atcatttctt catccgcagc
     8401 aaaaatctct tcatactgtg cggcggaatc ctcatacact tccttaatag aggaaccggg
     8461 cagaatatcg tggaactggt tcagcaaaag cagtttccaa ctgtgttcca gttgttcctg
     8521 tggatagaga tacttttgat ccagaagtga tgccattgct gcatacagct cggtttcgcc
     8581 gtttttgaac tcagccttac gattatattt tttattctgt gccatggaag tataagtacc
     8641 acggtggaat tccagatata gttcaccatc ccataccgcc agacgctttt tgtccatttt
     8701 gtcttccaga atatgaaaga attccttcac ccctgtctgt cttgctgcag gacattccac
     8761 gatgctgtgc tccagccttc tgctctgctc cagcatctct tctgtggggc ctccaccgcc
     8821 atcaccatag ccgtaacagg tcagcacctc agtgctcaca tccttatcct gataccgctg
     8881 ccaggtaccc atgagctggg aggcattctg cagaccatta taagtggtgg aatggtttcc
     8941 caccatcttc aggtcacccg gcttctgata gtttctggta gaaatcagat gtgtcaacac
     9001 ttccgttccg tcaatgccct tccagagaaa ggtgtcgtga ggaaaccggt tgaactcatt
     9061 ccatccgatt ttcgtggtca taaaataagg aatcccggat ttctgcagga tctgcggcag
     9121 cgccgcactg taaccaaaga catccggcag ccacaatact tcatttttct ccgcaccgag
     9181 ctcttgttca aaaaatctgc ggccatagat gatatgccgg atcagggatt ctccggagga
     9241 aatgttgcag tccggctcca gccacatggc tccttccgct tcccatctgc cctctttgat
     9301 ccgttccctg attctggcaa ataccccggg agcatcttcc ttcacgaact catagagctg
     9361 tggctgactg gacataaaac ggtattccgg atatctgtcc atcaggttca gtaccgtctc
     9421 aaagctgcga actgcctttt gtctggtctg acgcaggggc catttccatg ccacatcaat
     9481 atgggtatgg ccgatactgt gcaccgtcac cggattcggt cttctgtcgg cataataagt
     9541 accttccagc tcctgcgccg ccattttcat gctctcaaag cattctgcgg atccgggtct
     9601 gcgcagatcc aacagattta cgcatctttc cagtgcttta aagccctcga tcctggatag
     9661 attgtcctcc ggcagaagat ccgcagcttc atagacagcc tgcatggctg tatagagtca
     9721 cgccgccacg tcccgctgga ctgccagctc caggcggaag taattcgtag cggtttcagg
     9781 ggctgcattc gc
//
LOCUS       DAWDHD010000087         9767 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_418_length_9767_cov_11.356260, whole genome shotgun sequence.
ACCESSION   DAWDHD010000087 DAWDHD010000000
VERSION     DAWDHD010000087.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 9767)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9767)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9767
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_418_length_9767_cov_11.356260"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2849 a         1902 c         2745 g         2271 t
ORIGIN      
        1 gggtgcagaa aaacggtttt ttttttgctt tttgcatgat gccttttgat gtgtataaaa
       61 aagtcggaga agttgtccga tattctcaat agaggaaccg gtaaacaaac ggatttggca
      121 tttattttca aggaggagat aattatggcg ggaatcggag caaccggcag cataggtgcg
      181 tatgctgcgc agagtgatta cagcaagagt acgaagacga agaagtcgga ggcattcgga
      241 acaccggata agacagccgg cacaacaggt acgggaaaga cgtccgaaac ggcacagacc
      301 agaggaaaga ctgaggatta cggcagaacc atcggtcagc cggagctgtc ggagaaggct
      361 gcgaaatact atgagcagct gaagaagagg tacggaaatt atgattttat tctggtcagc
      421 agagatcaga aggagaatgc aaaagccaat gctgcaaaat atgccaatgg ctataagacg
      481 gttgttctca ttgatgagga caagatcgaa cagatggcaa cagacgagaa attccgcaaa
      541 cagtatgagg gaatcctgag cggtgccgct gcacagatac agcagttaaa gaccagcctg
      601 cagagttccg gtgcgcaggt caagggcttt ggaatgcagg taaatgatgg aggaacactg
      661 tcatttttcg ctgtattgaa gaaatccagc gccgagcaga aggcccggat cgagaaaaag
      721 gctgagcaga agaaaacaga gaaaaaggct gcggagaaga aggctgctaa gaaagaaaag
      781 gaagaacggc taaaagacaa gaagtcggat aaggtagcag acagcaagga ggaagccggc
      841 acagaggata cggtaacgat cagtgctggt tccatagaag aactgttgtc taagatagaa
      901 acctacaatt tcaacacacg gagtgacagt gtgcagaccg cagacgaacg gcaggtcggc
      961 cagaactttg acttcagagg gtaaggtgat gctgtatgat ttctatgggg aagtccggag
     1021 gtgtttacgg gagcggcaat accggtttgc cgcaggcatt ttccggagga aagaagacgc
     1081 agaacaatca gaagagtatc ttcggagcaa aaaataaaag ttctgttacc gatattcgta
     1141 gaatgctgtc cagaaacagt caaagtacgc agacgcaggc agcgaacagt tggggacagg
     1201 attccatcgt tacaagttcc ctgagttaca gtgaatcgct gcgcaatcag aggcagcaga
     1261 cgaagaacac gacgctggcg ctgaaaaagc tgaagtacca gtttaaaagt atttcgtcga
     1321 agatactgcg gagcaagact tcccaggcgg cgaagcaggc agccggacag gcgagaagag
     1381 agatcatgcg cctgaagcgt cagaagcaga acagtgacag cgacagtgaa gagatcgacg
     1441 cagcaattgc tcatgcacag gctatggaac gtgtggcgaa gaagaaagca aaacatctcg
     1501 aagaagagga aatgatgaag gctgccggcg gaatctgtca gggagaccgg atcagcgaag
     1561 aagaaacgaa ggatgtgcca gacgcagaag ttgaaaacgc gcggaatgca gaagagatgt
     1621 ctgcggaaag ttctgaggat gaaatgtcaa gggatctctc cgcatatgag tatgcaggca
     1681 gtgaggcgta tgcaggggat agctatgata tatccgacta tattgatctt ggcatagatg
     1741 cattctatgc gcaggcagga gactttatgt cggaaatgag cgatttcacc tcggagatga
     1801 tgcaggaaat gtccgatagc ttacgtgacc tgatggaaga gatggggctg gatggattat
     1861 cagataatgc ggtctctgtg aatagggaga tggatcccgc agacctcaag atgatgaaga
     1921 tcaaacaccg caacaaagag atgaaagaca tcgtcaaggc cgatgcggag tacctgaaag
     1981 cagttttcaa ccacctggag aaaatgaaag acaacgtggt cattcccacc gggaatgcag
     2041 gagcggtttc ctccggcatg acagggactt cattttccgc agatgctgtc ggcgtaggtg
     2101 tatcggcggc gcctgtgccg gtgatcaata ttacattata gggtggacat ataagaagaa
     2161 atttgacatc ccggacactg tgcttgtgag acagacacag tgtccgggat ggttttatat
     2221 aaaaacattt ttgattttcg tgaaaaacca cgtgaaaaca tttttggatt tcgtgaaaaa
     2281 ctatacaaaa acatttttga ttttcgtgat tatttccgcc cgtataggat tcccatgccg
     2341 gagatcatcc agggagcagc ggcatatccc gggataaaat ccagtttctt ctcaagattg
     2401 ccgatgtagt gaatttctgt gattccttct tttttcaatt cttcgacaaa ttcttccatg
     2461 tcaccgtaga gtgcttcctg ggagaacatg tcctgaaagg agaaggcacc gccctttttg
     2521 actatgcgca gagcttctct gaccacatcc cgtttatcct tggcggaacg gacttcgtgg
     2581 aagacaaagt tgctgacagt ggcatcgaag gtctcatccg gatcttccag atgggcggca
     2641 tctcctttgt ggaaggtgat ccggtcggca acaccctccg ctttcgcatt ggcttcacat
     2701 tgctctttgg catagttcca ctcggcaccc cagtagtcca taccgacgag ttttgccttg
     2761 gggaaagcct tggcgcagcg gatcgtcagg gcagccgcac cacatccgat gtcgagcaga
     2821 gtgccttcgc cgtcccattc cagatgatct acgagatgct gatggacttt tgccatcatg
     2881 ttgcctttgc cgaaggcaaa caattcatga cagatgtgca tgtaaatgcc atagaccagg
     2941 caaatcgcgg caagaatgcc caggatccat gtcggaatgg caagatgcag caccggtccg
     3001 gtcacatagg cggcaatgcc gagtacggca gcagcgacat agcacagcgc catcattttt
     3061 tcaggtaccc agtttccgta gatcacgttt ttgtccatat cttatgctcc tacttgtatt
     3121 cagaatataa caaaagttat atttgttatt ttttataaaa gaaaatgccc accgcattaa
     3181 cggtgagcat ccttctcgct cttaaaatat acaattaaac cctgcgttcc ggcatcggcg
     3241 tgaataccga tggctttcgt caggaacagg cggtgttcca tatcataatc tcctttgatc
     3301 aattccagaa atccgtgtaa cctcatggat gtcagaatcc gtagtaattc tttatcgggc
     3361 acatagccta gttgtttttc gtaatagctc tgaaaggcgg tgaacatatt atgaaagact
     3421 atatcccgga aggattcgta tttgctgccg gagcatttct ccagtaccag ctcacattcc
     3481 tgcctgtgcg cacagatata ttccatgatc tcgcgttcat tctgctctgc ggtctcagga
     3541 tgttcctgct cccttttggc tattttctgc atcattttct gatattcggc aatcagggga
     3601 tccagaatcg cccccagcag agcttcctta ttcgcaaaag agaaataaaa agctccggta
     3661 gtaactccgg cccgtttgca gatatcccgg aggttcgccc cggcgtagcc tttttccaga
     3721 aattcttccc tgccgcagcg gattaagttt tcttccgtga ttttcttacg atctgtctcc
     3781 ataacaattg ttatattagc atagactaac tcgagggtca agaaggaatc ttcaggtaaa
     3841 atattttcgc aaaaatctct gcaaaaacat tttggtttat tgtgattata agatataata
     3901 aaaatagtag caggaaaact aagatagtga tatatagcgg ggaaatgaat atggacaaag
     3961 acccatttaa agaatatctg agagaatccg aaccgaataa agcaacaaaa gggtatgtgt
     4021 ggagcacggc ggttggactt caggcagtag atggtctgaa accctcgcaa tatctgattg
     4081 acactgcaat tcaaaatatt gaaggtaaga tcacgctaaa agaggcacag agtcttatag
     4141 aaagctatta caatgagaga cctgtccgtg tatcagataa tgaacgcacg gaagaagcag
     4201 ataaagtttc cagccgtatt gcggagctcc tttccgaaac ggcgttttcc ttttcaccga
     4261 atgagtatat agctattcat cgtaagcttt ttcgtggaat atataagcat gccggaaaaa
     4321 taagagatta taatattacg aagaaagaat gggtgcttga tggcgcaacg gtcgtttatg
     4381 ggagtgcttc agaactgcgt gctacattgg aatatgattt ttcgcaggaa aaggatttta
     4441 gttataaagg actttcgata gaagagagca ttcatcatct ggctttgttt gtatcaaggc
     4501 tatggcaaat tcacattttt ggtgagggaa acacaagaac cacagcggtt tttttcataa
     4561 aatatttgag aacacttggt ttttttgcaa caaatgacat ttttgcggaa aatgcatggt
     4621 atttcagaaa tgcacttgtc agggccaatt atacgaattt acaaaaagga attcatgaga
     4681 caacggaata tttggaactc ttcctcagaa atatgctttt aaatgaacac aacgagctgc
     4741 acaatcgaaa tatgcatatc agtggacttt taaaggatac aaaagtggac attggaacat
     4801 caaaagtgga cattggaact tcaaaagtgg acattggaac tcaaaaaatg gacattgaaa
     4861 catcaaaagt ggacattgaa acaagattgg ctgaaaaaac gctgaatttt tcaacaaaaa
     4921 caattatgca tatccgtgta ttatatgaaa aattcggaga ttgtcagttt tttggcagag
     4981 gagaggttat ggaattgctt gggctgaaaa gttcaggcgc ttctaaaata atatccaata
     5041 tggtacaggc tgatgtaatc gaaccggtat ccgggcatgg aaaaggaaaa tacaaattca
     5101 aagaattata gagtatattg gatttaaagg catcggaaca tccggtgcct ttttttaaaa
     5161 tacaggaacc tttttaccgt ccggcgactc atatagtatg aagccggcga agagaaggag
     5221 gtgttacttt gtcagttatg acaaaagagg aatttgtgcg ggaagtgcgg ggctataccc
     5281 tgcatatgta ccgactggca tattctattc tgaataatag tacggatgcg gaggacgctg
     5341 tcagtgaagc ggtgctgcat gcctatgaaa aattaaggac actgcgggat ccgggcagct
     5401 ttaaaaactg gatcttacag atcacggcaa atgaagcgaa aaagatttat cggcaaaata
     5461 agcggaccgg tgcggtggca tgggaagaga atctgtcccc ggcattttat gacgaacacc
     5521 atgaactgtg ggatgctgtc atgcagctgg atggcggata ccgggatgtg atcgtactgt
     5581 tttactatga acagtgcaca atcccggaga tcagcaggat attggaatgc aggcagggaa
     5641 ccgtaaaatc gagattgtac cgggcgaagg agcagttaag ggagaggctt atatgagaga
     5701 tgattttata gacagaaaaa taagaaaaat ggcggaggcg gaacggatgg cagtgccgga
     5761 gtctctggat gcgaaagtgg aaaatatttt ggacggactg gaaacagaga agaaggacag
     5821 caaaaacacg gatggaaaga gagtgaatca aacggtacgc cattatggct ttagacgaat
     5881 cgtgatcccg gcagcagcct gtctgcttct ggcctccgtt accgtgacgg catcgggatt
     5941 gtaccgggcg cggatggaga gcatgaatca tgaaaaactg gaaacatatt tttccgggtt
     6001 gcaggaggca gatgttgcag cggatagcta cagcagaccg cttacggacg gcgaaaaaaa
     6061 caggctggaa gaactgaggc aggcctatct ggaggaagga tattttccga aaaaggaact
     6121 tgtcatgctt gatagtcctg aaaaatacaa aaagggcgtg gctttctatg cagcgcggag
     6181 tatatttttc ctgccggaag aggaaatgac ggatgaggaa ctgttggagc tgatcgattt
     6241 ccgggaaaag agagattata gtctggcgaa aatcacagaa gagatcgagg cgggcgacta
     6301 tgaatatgtg aagacggaag atagtgcatt accgggtgat atgtcacagg agagcggatc
     6361 gcaacagaca cagggcgcag gagaggcaag tgtagtcccg ggggcagaca ctgcagcaca
     6421 ggaatggcag attgcctatg cgggagaact cagtatccgg tgtgctgtgg cggcagaaca
     6481 ggggctgtat gtcggtgggt tcaccatgga agggaaagca cgggtggaat atctgactta
     6541 cggcagtggg accccggaaa agttttatga tgatttcgat gaggacggcg aggtgtatag
     6601 cctgtgcatc gcaccggacg gaagtgtcta tgtggcatta cgcggactgg catctccggg
     6661 agcggagcac ggctggctgc ctgtaatcta tcatattacc ccggagggaa cgcttctgaa
     6721 cagttttact gtagaagagc gggaatggaa tatcgttgat agtatggcga tagatgccca
     6781 gggcagatta tatgtgcgga tgcggatgca ggaggacggc agttatgtgc ggatctattc
     6841 tgcggaagga gaactgatcg gaaacgtggc ggatgacaac agctatgccg tacaaggtgc
     6901 cggagcatta ggacagggca aggacggcaa agtctatgcg gctgcacagg cagtgggcga
     6961 tacgacagca gggaatatag ctatgacatt agtgtcagta gatccggaaa gcctcaggtt
     7021 tgttccggtg gctggaggtg agaatgttgt ttgcagtgcc gatacgatct acccgtggaa
     7081 aatggtggca gcaggtgtga gtgagacggc agattttatc ctatggggat acaacggcgt
     7141 ggacacctat cgtttgggtg agacggaatt caccagagtg caggagaact gggagatgcc
     7201 ctgcggtacg gaaggtacct gtgcggtcct cgtaccggac ggtcgcattc tctatatcgg
     7261 cgcatccgac ggcttccagg gaacgacggc ggacggcgtg gagatcagcg gaaaagatcc
     7321 tgagaagaca gtgctctatt atgagccact cgtaaaatag agtgacaata aactggcgac
     7381 accgggaagc cgatgccgcc agttttatag ttccagactg ttaagtttcc atctgctcaa
     7441 ttgtgcattt acagtggtta tgttataaaa agaccagaaa taagagaaag cggtcgcttg
     7501 ggtgaaaaaa gtagattcag gggtgaaatt ttgttatact gtttgtaaca aacggagtat
     7561 aaaagtcgtc aatatagata gaagggaaac aaaggagggg cttatgaaga agattaacgt
     7621 aaaagcaacc atttttctca gtgcaatcgt attggtggta gtggtacttc tgctgaatct
     7681 catcgaattc cgcaaaacgg ttgatttcac ggtgccgggg atacagatgc cgaaggagaa
     7741 cgctttcgga aagcaggaag aggtacaagt gagcgtcaac ggaacctatt attaccgggt
     7801 actgccctgg attcacggca gcactttcca tgggcagata tctttcgttg gagagggaga
     7861 gaaaccgcag aattatttct ttacctatgg agcaatcaga ggatttctga aggagggaag
     7921 aggggctggc tggatcggaa ccccggatgc tcagactacc gtagcgattt atcaggcggt
     7981 acagaatgtg gaaaaaggga tgatatccgt agagaatccc gccagctacg ttttatatcc
     8041 ggcggcaaca ccggaagaag cctatgccat agcggatata tggtatccgg gaatgctgga
     8101 gaatgaagca aagacagcat tctgcgcggc ttttttgccg gaggaatgcc ctcagaagat
     8161 tggctgcagc ttctgcgcgg aggattacga ttatgcggaa gatgtcgata ccgtagagca
     8221 gctgtggcag gaatttatac aactttccgg aaaagtgacg tcagatccga tcccgccgga
     8281 agagatggtg gaaggtgtgg gatattttca gtttgtctat gcagacggtg agacaaggat
     8341 ctttcgctac gcgtcggaag aacagggatt atggataggt gaggacgaac gctgcctggt
     8401 ggacggccag gctttctatg actggcagga gcgcctgatg gaagcgttgc agaagtaagc
     8461 acgggaaaga gaggggcgga aatcttctcc attcgcagca gatcccacat cggacagggg
     8521 tatgcgaagc agtatggtga ggaactgcgg cagtattgga aagcaaccaa tgaggttttt
     8581 gtgtggatca gtgaacagta tcagaaaccg ttatatgtca cttcggatgt gaccataggg
     8641 gatggaaaga cagtgatctc gttccggggg accggcactt ccatgactac cggggagaca
     8701 gaggaaatcc atagagactg cgtgctggat tttgaaatgc atgcggaggt attgcgctcg
     8761 gaagaagagc cggagctgac agtaagtgac tgaaatccgg aagaagaggg acaacagaat
     8821 cagatttctt acttgacaga taggtcggta gaatgtagac tataattaca aagagtctat
     8881 aaaatatcga ctatgccttg ccggggggca ggacaggagg ggcttatggg tgagaatatc
     8941 tacagaatat ttgacggtgt atggaactta agtctggtgg gaggatactg tgtgctgctg
     9001 gtgatcctcg ccagactttt gttgcaaaaa gcaccaaaat ggtgttccta tctgttatgg
     9061 ggaatcgtgt tcgtgcggtt gtgctgcccg atactaccgg agacaggaat cagcctgatt
     9121 ccggagaaat tgctgtctgt ggggacagag atgacgtccg ggcagcatac agtcggtccg
     9181 ggaagtcatg gttatgcaga ggccaacgat acgaaggata tcggagatgt cctgcggcgg
     9241 gagtacgatg cagaggaagt accggacagc cagacggcag aaaaaacgat ggctggtgga
     9301 aatgacaatg ctgtcatgaa cgctgacggc acgcaggatt tttccctggg aactgcgaga
     9361 acagaaagtg gagctacggt tgcagggggg gcaaccagtg gaacagatgc agtgatttcg
     9421 ggaacatctg ccggaacggg tactgacgcc cgcgcatgga catacccggt gtggttccgt
     9481 gtattatcca tggtatggtt ggcaggaatg cttggcttcc taggatatca tgcttattct
     9541 tatctgcgca tgcgtcatcg gattcgtaga tctgacaata gtgtcaggca ggtggaacct
     9601 gggatctgcg agatcgacgg tgggcatctg tccttcgtca tggggctgat ccatccggta
     9661 atctatctgt cttcgggctt agatcctgag agcagaaaag ttgttctgtg tcatgaacgg
     9721 gtgcacttac agcgcaggga ctatctgttt aagcctgcgg cgttggt
//
LOCUS       DAWDHD010000088         9650 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_424_length_9650_cov_14.216780, whole genome shotgun sequence.
ACCESSION   DAWDHD010000088 DAWDHD010000000
VERSION     DAWDHD010000088.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 9650)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9650)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9650
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_424_length_9650_cov_14.216780"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2459 a         2416 c         2035 g         2740 t
ORIGIN      
        1 tcttccacgg cgatgttttc atgttatctc tccttctgtg caatcattgt ttcttgtgtt
       61 ctgtattaga gaactctgtt ctctaaaata tctttttgaa aaggattgat ttctcaatcc
      121 cctttttcac atttttaact taataaagaa tgttttgtaa ttcccgtatt ttattttgta
      181 attcctttgc cagtgactgg atccgttcgt catagaatct cagggaaggt cctgtgatac
      241 tcactcctgc caccacctgg ccgtttctgc caagtaccgg aaccgccaca cattttacac
      301 caaattcatg ttccatattg tctattgcgt aaccacgctg ccggatctcc cggagttcct
      361 tcaacagtac atctccatcc gtgatcgtat aatcggtata ggcttccagc ttcctggctg
      421 cgtaggcccg ctgtttctcc tccggaagat acgccaacat tgccttgccc agcccggtac
      481 aatgcatctg tgcatgttca cccatgatcg gccgcataag gtcaatggag gtcttcggat
      541 acgcagaagt cacgtagata atctcctgtt catgtgggat tgcaagatag actcgctcgc
      601 ctgccatatc tgcaagctcc tgtaaatacg gagctgccac actaccgatg cttaagttct
      661 gtcccagaga ataacacaag gagtacacac tgagtcctag cttgtatctg ccactctcat
      721 catcctgatc cagatacccc attgccttga acgtagacaa aatattatgt acgttacttt
      781 tattcagatc cagctgttcg ctgatctctg tcacacccca actgcttttt ttcgtaaagc
      841 agttcaaaat ttcaagagct ttttgcagac tctttacttt tatttctgcc tcatttacca
      901 tcttgcgttc tccatttggt aattgtgttc ttcatttcgt aaccacatta tataattttt
      961 aaagttaaaa aagcaatagc acattttgat aaattcgtct tacatctttt gtctataatt
     1021 aacttcttgt tctgtaatag agaactttct cattacaaaa tctcttattt acattaatga
     1081 tagcacaacc tctttcgttg tgatatgatg gaacaaaaca gtgttcgaca atcttcgacg
     1141 aaagaggtgc ccctatgaca ataacctggt tatgcctgtt ctggttgttt ttcttctatt
     1201 cctttattgg atggtgtatt gaggtatgtg ctgctgcggt gcagcatcgg aaattcgtta
     1261 accgcggatt cgtagcgggt cccctatgcc cgatctacgg ttttggtgcc atattgttcg
     1321 agtttttcct gccggaacta acggaagacc cgtttttcct gttcctgggt ggctttgtac
     1381 tggcttccct gttggaatat tccaccggca tgttcttcga aaaggtctat aagaaaaagc
     1441 tgtgggatta ctcccgtttc cgctacaacg taggtggcta catctgcctg cctttttccc
     1501 tgctgtgggg tgctttaagt gttgtgacgg taatgtttgc agatccgtta ttatgcggac
     1561 tttttgaccg cattccgcac ttgctttccg ttgtacttct gttggtgctg ggcgggctgc
     1621 tcctgttgga caccgttggt tccgctttat ctactttaag cctacaggta caggcaaagc
     1681 accgggtgga ggcctctctg gaaaagcaga ccgcccgcct ggatcagatt acagaagggc
     1741 tgggccagac ttcccgtttt ctggaaaatg ccctgacaag acatatacag aagcgactgc
     1801 agaaatcata tccttctatc agcctggatg ctctggtaaa agccagagaa gaacataaaa
     1861 aatccaccgt ctttgccgag ggctgctgct tttacaaact gttttccctc ttcttcatcg
     1921 gtgcattgtt aggtgacatt acggaaacga tcttctgccg catcaccgca ggtgtctgga
     1981 tgagccgcag cagtgtcgtc tacgggcctt tcagtattgt ctggggtttg ggctgtgcct
     2041 tccttaccct gctgctctac cgttaccgca acaagagcga cagtactatt tttctggcag
     2101 gtaccctgct gggcggtgct tacgaatata tctgcagcgt cttcacggaa atggtctttg
     2161 gcacgatttt ctgggactac agcggatttg cctttaactt aggcggccgt atcaacctct
     2221 tgtactgctt cttttgggga attgccgctg tggtatggct gaaactgatc tatccgaatc
     2281 tttccggctg tattgaaaag attccgaaaa gaaccggcac aattgtctgc aatattttga
     2341 tagtatttat gattgtcaat atgctgatct ccgcactggc gctggccaga tatacggagc
     2401 gcaacggcgc ttcctattcc gtggaaacta cagagcttaa cggactggaa caatttttag
     2461 acacccatta cccggatgca cggatggaac gcatctatcc caatgcaaaa atggtgaatt
     2521 aaataatttt gtaaagatgg gagaccacca gacttatgca gatcgatgac ttgacagcag
     2581 ggttatccct tagctttttg atcaatctta ataccgagca gcttcaattt gaatccaaaa
     2641 ttctggaggt atatcctcac aggcacactt tgctggcgca gggtgtgttt actgatgata
     2701 aaattatctc tttccgtgga aaaggcctga ttgtagatct cattgtcaca cttccggagg
     2761 ataaaccgca gctgttcaaa aacgttacta ccaatgtagt aaagctttcc gatggaacac
     2821 tgtgctatac tgtctcctcc attgccggaa gcaagaccta taaccgacga caaagctacc
     2881 gatgctatat tggcattgag actgccgtac aatgcggtct gaaccgtgcc gcccacgatg
     2941 ccattattaa ggatatcagc tataacggtt tttccgtggt cagcagggaa gacttgaaat
     3001 tagaggtcaa ccagacggtt catgcggttc tgaatgacca catggacgag accgctgaga
     3061 atttcaactt ccatctctat ggactggttg cacggataca ggaactggaa aacgggctgt
     3121 ttctctacgg ctgccgtctc aacaatccgg ttccgggact ggaacaatat attataaaaa
     3181 aagaacgggc tgtcttacgg aaaagacagt catcctgaga ttctacgcaa ctatttcgta
     3241 taacgcaaaa aaccgctgcc ccttacgggg caacggtttt tgtattcaaa ggattatccc
     3301 tcaatagcga tgtatttatt ggtctctacc gcaggttttg cttccttctt aggaacattc
     3361 agagtcagga taccatgttt gaactcgccc ttgatatcct cctgagtcag gttgtcacca
     3421 acatagaagc tacgctggca agctccggca taacgctcct tgcggatgta tttaccggtc
     3481 ttcttctcct gctcatcctc atccagacct ttggcagcct caatggtcag ataaccatcc
     3541 tccagagaaa ccttgatctc atctttcttg aagccgggca gatcaacgat caactcatag
     3601 ttgtcatcgg tctccttgat atcggtcttc attacattat gtgcatgtct gccatacagt
     3661 ttcttctcta tctgattctc tgctctgttg tcaaagaaag gtacatcatc aaaaaagtca
     3721 tcaaataagt tctctccaaa aatgctaggt aataacataa gtcatctctc cttttctatt
     3781 aaacatatgt cggtgtcttt cgatacctct taccttttgt cctccggcaa ccaccggaga
     3841 atttcggaac ctgtcagagg atcttcgatc aatctcttga tctttcttcc tctcccttgt
     3901 tctatttgta ctataacacg catatataag tatgtcaatt aaggaaaaat tatataatta
     3961 taaattaaaa ataaactgca aaaaagaacc agaacactgt gatccacagt actccggttc
     4021 ttttttacag gcaaaaccgc cagtcttact tatttgtttt ctcttactta tcctttgcag
     4081 taaagaatac gaagaataca tctccctcag tgtcattgga gaactgctcc agataagaaa
     4141 tggaataatc cttagaatct tccggtactt catataccag cagaccggtt ttctcttcgt
     4201 taacacccag atcatactct tccggcagct gttcgtcact tacagtgtta ccatagtagg
     4261 tgataggtgc agcgtatgcg tcatcgttat catcatcgcc ccactggatc tggaaatcgg
     4321 tgtcatacat ggtaacggta gaacgatcgg tgttcttaac ggttacttct gctactaaca
     4381 gcttatttcc ctcggaaggg gtatatcctt catagtcatt gcaggtatat gcgctgttta
     4441 cagtgtaatc gaagaagtaa gttctcatta catctcccat acgtccttct gcatagccgt
     4501 catcatctgc ggttaccaca ccactcttgc tgctggtatt ggatccggaa cttgcaatat
     4561 tctgaattgc gctgtctaca ggaccacatc ctaccagact aaatgccagt acagttgcta
     4621 caataattcc aacaaatttt ttcattttct ttctccctct cataaccctt attgtgcatg
     4681 cagtcatctg cacgcgaagc tagtataaca cacaaaatgg cagaatgcaa cgctttattg
     4741 cgctactttg cgaatgtaag tgcttactca cgggtcagac gcaggatcac atcgctgtgt
     4801 tccggatatt tttccaatag ttttttccgt tcaaacagtt caaattcttc atatttaaaa
     4861 cagggtgaaa ccatacagcc caccagactg aaaccctctt cctccatggc agacccgaag
     4921 atcattccct tcggaaccag tacctgcggg acttctccct tttccaggtc cagcccaagt
     4981 ttcgccgctg tgagttttcc ggcctcatcg atcatataaa tcgtcaacgc attgccgtca
     5041 tgataatacc atacctcatc cgacttcagg cgatgaaaat gggacacttc cccctttgcc
     5101 agcataaaat aaatgctgct ccacaggttc ctctcttctt tttccagcag gcattccttg
     5161 aaataaccgc cctccacatg gggctccatt tccagatgct ctatataata ttccgctgaa
     5221 tgcttcatac tcttatccct ccggctattt tcttttctac tgaattagtg tatactatgc
     5281 agagaaaaag cgcaattatt aacctgaaag attgtgcgga atggagaata tcatggaaaa
     5341 aatagcaagc ttcaccattg accatatcaa gcttcagccc ggtgtctatg tatcccgcaa
     5401 ggatcacatc ggtgcagagg tcatcaccac cttcgatctg cgtatgacca gtcccaacga
     5461 agagcctgtg atgaataccg cagaagtaca caccatcgag catctgggtg caactttcct
     5521 gcgcaatcat ccggaataca aggacaagac cgtctacttc ggccccatgg gatgccgtac
     5581 cggcttctat ctgctgctgg ccggagatct ctcttctaag gatattgttc ctctgatggt
     5641 ggaaatgttt gaattcatcc gcgattacaa gggtgaggta cccggtgctt ctcccaaaga
     5701 ctgcggcaac tatctggata tgaatctggg tatggccaac tatctggcaa aacgctatct
     5761 ggataatgtg ctgtacggca ttgacgacag ccgtctggta tatccggagt gattacaatt
     5821 atttaagaaa gaacaggaaa cacacatgaa aaaaatcgga atcatcggtg ccatggagct
     5881 ggaagtggaa actctcaagg ctcacatgac caccactaat atcacgacca aagcaaatat
     5941 ggaattccac gagggtaccc tgaacggtac tcccgtagtc atcgtccgca gcggtgtcgg
     6001 taaagtcaat gccgctctct gcatacagat tctggcagac ctttttcagg tcactgccat
     6061 catcaacacc ggtgtggcag gatccctgaa tgccgctctg gacatcggag atattctgat
     6121 ctccaaggat gccctgcacc acgatgtaga tgccaccatt ttcggttaca agcctgggga
     6181 agttccccag ctgggatgcc gggaattcct tgcagatgag catctgatcg aggttgctgc
     6241 cagcacctgt caggaggtca atccggacat tcacgttcat accgggcgtg tagtcagcgg
     6301 agatcagttc atttccagca aagaagtcaa ggaccgcctg atcaaagaat ttcaggggga
     6361 ctgtgccgag atggaggggg cttccattgc ccacggtgct tacctgaacg gtattccctt
     6421 tgtcatcatc cgtgccattt ccgacaaggc ggatgacagt gccgagatgg attatcctac
     6481 ctttgaaaag gccgcagcgc tccactccgc caagctggta gaggctatga tgccccggat
     6541 ctgatccggg cggctgtcct gtggatcgaa aacaatgcct gctgcacaga ctttgcaggc
     6601 aatagaaagg catgcatatt aacatgaaaa aagaaaagaa atacattgaa tatatcttaa
     6661 aacaggccga agccctgctg gctatcgaca gcccttccgg cttcacggaa aaagtaacgg
     6721 attacctctg tgaagagtac aagcgcctcg gctacaaacc ggtcaagacc aaaaagggcg
     6781 gcgttctcgt agagatcggc ggtaaaggca acgcggttct gaccatggcc catgtagaca
     6841 cgctgggcgg catggtggca gagatcaaga gcaacggccg tctccgtctg actaaggtcg
     6901 gcggcttaaa cgccaacaat atcgagaccg agaactgccg tatctatacg ctggacggca
     6961 aagtatacga aggcaccgta cagctgacgg atgcttccat ccatgtcaac ggcgattacc
     7021 agaagtccga gcgtactttt gattccgtgg aagtcctgct ggatgaaaag gtatcttcca
     7081 aagaggacgt aaaagcactt gggatcgaaa atggttcctt cgtctgcatg gatccccgga
     7141 cacgtatcac agaatccggc tacatcaaga gtcgtttcct ggatgacaag ctcagtaccg
     7201 ctattttatt aggctttgca aaatatgtaa aagacgaaga cctgaaactg tcccgcaagg
     7261 tctaccagca tatcaccgtc ttcgaagaag tcggtcacgg tgcctgcgct tccgctccgg
     7321 aagatgtgga ggaactgctg tccgtagata tgggttgcgt cggagagggt ctggaatgca
     7381 cggatcgtca ggtatccatc tgcgtcaagg acggccacgg accttatcat tatgatgtgg
     7441 tcaaagggct gatccaggct gcaaaagaca atcatatcga ctacgccgca gatgtttacc
     7501 cctactacgg ctccgatgct gatgccgcat taagcgccgg tattgatgcc agacacggac
     7561 tcatcggccc cggtgtctac gcttcccacg gctacgagcg ttcccacaag gatgcagttg
     7621 ccgcaacgct ggaattgttg aaggcttatc tggcataact ccaaatataa atcgcacata
     7681 aaaagcagtc cggagaccag cagattttct atcctctgcc agttccggac tgcttttata
     7741 atactcttgc aagttaatat atttattttg cctcttctgc tccgccgtct tttttcttgt
     7801 tgaatacgaa cagcttgctg aatacatagt tgccgatgat gaccagaact gcggagatca
     7861 gggttgccac cagcagattt acaccgattg cggtcagtac ctgcatcact accatatcca
     7921 ggatcagcgt ggatactctg gaaagtacga atttcggtgc ttcggacagc atcggagcat
     7981 tgctcttgaa tacaaactta cggttggtaa aatatgcgaa aatgacaccc gccgtccagt
     8041 tgataacact taatacaaca ttctgaaagg gtgtgggata cattgtgttg tcaaataaca
     8101 ggaagtttgc caggaatttt gctgcccagg atacgaccgt ggtcagtccg cctacgatca
     8161 ggtatgcaat gatctcttca tacttgatcc aaagtgcctt tattttttct accatattgt
     8221 tcctcatttc tctgtgctaa ggttaataaa actattcaga accccattcg caaaatcgcc
     8281 tctacgaggc tttctttttg ctcatgtggt tacttcgccc tataaatagt taactacaaa
     8341 taacaaatat acacaaatat ccccttaaat gcaagcattc caaagacatt tgtgtatatc
     8401 ttaatcactg tttatgaaat tctataccac cgtatggctt ataaatttac cacgttacgg
     8461 ttctcaccac gcataaatgc cttaatgttc tcgtagacgc cctgtacgca acgggtacgt
     8521 gcctccacgc tggcccatgc caggtgcggt gtgataatca gcttgttact gtcttttaac
     8581 tggcttaaag gattagataa ctccaaaggc tccttttcca ggacatccag acctgctgcc
     8641 tgaatctctc cggccaccag tgcttcatag agagcagcgt tatttaccac ggggcctctt
     8701 gccacattga tgaggattgc tgtctttttc atcttacgaa gggcttcctt atcaatgaaa
     8761 ttacgggata agtcacttaa cggacaatgc agggacagga aatcgctctc ttttaacagg
     8821 gtatccttgt ccacctgcgg ataatccgta caggtgctct tgcccgtgat agaataaaag
     8881 attaccttac agccgaaagc ggtagcgatc gctgcggctc tcctgccgat attccccatt
     8941 cctacaatgc cccatgtctt accttctaat tcataaaaag gtctgtcaaa attagaaaaa
     9001 cgatcctgtg cactgtaagc gccggatttt acatattcat catagtaggg cagcttctgg
     9061 ctcaatgcca gcgccagagt aaaagtatgc tgtgccacca tggctgtgga atagtccacc
     9121 acatttgcca ccttgatgcc tctggacttg caatatgcca gatcacagtt gtcgtatccg
     9181 gtagcaagct cacagatcag cttcacattg gggcaatcct tcagggattc ctctttcatg
     9241 ggagccttgt tgcagacaat gacatccgca tcgcccacac gctctgccac ttcctgtgca
     9301 gtaacggtat tcgggtaact ggtcacttct cccaattcat taaagcagtc caccggcaca
     9361 tccgtgccgg cgctgtttct ctccagcaat acaatcttca tctcttgcaa tttctcctgt
     9421 cttttgtttt cttttcgtaa tagtataata acatagtaga ataaacgatt tctccgggct
     9481 atcgccctac ttacttattc catcatacag caacagcgta atgaaatcaa ttcattacgc
     9541 tgtccttttc ttcttttgaa acatattact gccagagtga tcccgcctct gtggcttaca
     9601 atctcttcag cagttcttct ctctgtgcct cataaccggg cttgccaagc
//
LOCUS       DAWDHD010000089         9539 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_430_length_9539_cov_9.252003, whole genome shotgun sequence.
ACCESSION   DAWDHD010000089 DAWDHD010000000
VERSION     DAWDHD010000089.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 9539)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9539)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9539
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_430_length_9539_cov_9.252003"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2270 a         2603 c         1942 g         2724 t
ORIGIN      
        1 cggtctctga ccttggccca cgaaacaggg gtccctcaca ttgctggact tgccgctggt
       61 atccagcatg cggctttcta ccacagcggt cacctcccgt ccctccatgg ataatgccat
      121 ggtggacgta ccgaagaagt ttttcgcctc tgtgaatttc accgccagtt cctgcaggat
      181 ttcttccagg gaagcatcct ccctcatcag aagagtgatc ccgctctgat agcttttgat
      241 caatactgca tctttcattt tcttatacct gtctttttat tttcttaata tccgaagtcc
      301 cacatttggc attcatctgt ctgtgttatc tgactccgga tagttacatc tcattggata
      361 ctgcattgtc gagagtatcc gcagtacctg tgatcagatc atcctcttcc gccagtccgt
      421 aataatactt gataatatct ctggtgaact gggctgcata gtctgaagaa tatccgaaag
      481 gtattcttgt ggcaatggcg atctccggat tctcatacgg cgcatagcag ataaaaagcg
      541 catggtttgg tcgggaagct gtctgctctg ccgtaccggt cttacctgcg gcatttaccg
      601 ccagatcact aaagtacttt ttgttttcca ccacctgacg cattcccaga tggaacgcat
      661 cccagtattc ctgcggcagg tcaatctgat ttctcacttc tgcatggtac tctttcaaca
      721 gattgccctg ggaatctgtt gtcttatcca acagggtcag attatagcag gtcccactgt
      781 tggcaatggt agtgacatat ctggccagtc caacggtagt gtagctgttg ctgccctgtc
      841 cgatggcaga acgtaccgga tccttggtgg atacatccgg tgcatattct ccgatctcca
      901 ctcccgattt atctgtcagt ccatacagat ccgcatattt gctcagggta tccagacccg
      961 cttcctcatt atagcttccg gttctggtag ccagctggta gcccacattg tagaagaagt
     1021 agttacagga atccttgatg gcagatgtca cattttcata gccatgtccg gaaattttcc
     1081 agcatctggg ggatggtgtg atctcggtaa aagtaccgac acagttcaca cggctctgaa
     1141 gattaatcac attctccaga agtcccgctg tagcggatac aatcttgaac gtggaaccgg
     1201 gtgctgcctt atactgggtg gcaaaattca gctgtgggct ggacttgtcc gcattcagct
     1261 gcgcataata ctccgcatcc acggtattcg ccattttgtt attgtcatat cccggataag
     1321 acaccatagc cagcacatct cccgtattga catccgtaat gactacagag gcattgcagg
     1381 gatccagtgc cagttgggcc ggtgtgatct ccagattatt gatccggttc atcataaact
     1441 gatatgccga caatttaccg gaatacagtg tttcttcatc ctcagccggg atgtccacca
     1501 cctgctgttc acacaggagc atacagatct gtttcccggt gatcttgtca tttaaaagca
     1561 tatacttgta gaaacgtttc tggaactccg tggtatggtc gatggcatta atggtgtact
     1621 ccaacagttt gtcataaact tctgtagaat ccgaatattt gtcattcaga tccagcttgc
     1681 tgacatcgat ccagttcatg gcgatgcaat actgcagata ctcctttaag gagatcactt
     1741 cctccttcgc ccaggccgtc tgcgtagcat cgttatcatc caccagttcc ttcatgatca
     1801 cgccttcctc ccgaaggagg cttacgatat tactctgata aacctgatat tccttggaaa
     1861 gcttgctata gggagttgcc ccttctgtaa gttcgtaagt cagtctgtcg taaacaccct
     1921 gcttatactc cagataagcg gcatagactg ccttttccgt ctctccggca tcttctgcct
     1981 cgaaatgttt tctgtccagg atattgttgt tgatcatggc aaaatacacg tcatagatcg
     2041 ggatcttaat ggacgagctc ttggcattct ccggcagatt gaattctttg gcgttgatga
     2101 tcttgcttgc caccagaccg gccagcttct gttcgatgat attatatacc gcctccgtaa
     2161 gatccttatc gatggtcagg tagacatccg ctcccggctg tgcctctttc cgctctaaaa
     2221 tagtgatcac cttaccggta ttgttcacca ccactttttc actgcccttg gtaccctgca
     2281 gggtagtctc catatatgcc tcaataccac tcttacctac cacatcattg atggtatagg
     2341 tatcctccag gccgccttcc gtcaccacct ggtcatttaa ctctgccagt tcatcggaag
     2401 agattttgcc ggtatatccc agcacatggg cgaaatattt gctgtctatg tagtggcgca
     2461 ccgtatcctc tacgatggac acaccgtcca gctggtcgga attctccatg atcaccgcca
     2521 ctgtctccgc actgacattg gtagccacag tggttcccac atatttccgg aaggaggtca
     2581 gattcatggc ataccggatg gttaccatct gcagccactc ctctttggta taacctttcc
     2641 ccggcacgaa gggggtcttg gtgttctcgg gatcttcgta ctgaccgata gcaaagccct
     2701 tggcacggct taagtactct atcagttcct gggctgtcat ggctttttct tcttcactaa
     2761 gctcgtctat ggttctgtat ccaagcacat ccgcctgaaa acgaagcagc gccgtgccat
     2821 ccacggaata ggcataatcg ccgtcttcat caataatgat cttaaaatcc gtgatcacac
     2881 tgtcaccgtt tttctcgatc agctttatca gccgatagac aacgtcattg accttttcgt
     2941 tcttattccg gctggactca aacacatcct cgatcttcac agaataagcc agttcatttt
     3001 ccgccagtac attgccattc ctgtccagga tccgtcctct ggtactggat atatccctgg
     3061 tcttctcgat ctgaaggata aagtcgttca gatactcttc tccgtttacg atctgcaggt
     3121 tgaaacagcg gtaaagcaga acaccggcaa aaaaagcaaa cagtatcata agtactgtca
     3181 gtctgctggt cacaatatta ataatgtgat ctctaatttc acgaaacaaa tttctgtgcg
     3241 ctcctttttt ccttctcctc cagtttctga ttaatcttca ggatcagagg atacaaaata
     3301 atcgtaacaa cgatggtgta aagtgcctcc ggcaggatca cacttgtaaa ataatacggg
     3361 aaatgaaaac gccctctcaa aagaaacatc agaatgtaac aaatcaatcc ataagaaatg
     3421 tcgctgacaa taataagcgc caacggcagc ttgatatcct ccggataaaa gatccggctg
     3481 aatttcccgt taacaaagcc tatgtacatc atgaccatgg cataaaatcc caggaaactt
     3541 ccgaaaaaaa tatcacataa aagcccgcag gaaaatccga tcagcagtcc ctccttctcc
     3601 cctctcataa atccgaagga agaagtcaga atgatcatca gattgggaat gattccgccc
     3661 attgccaggc tgttaaatac actgcactgc aagacaaaaa ataaaaggat cagaagaacc
     3721 actacaatct tgcgcaggaa gctgctgtta ttctccatct gcgcctcctc ccggaacctg
     3781 ctgtttcttg tccgtgatca ccagcacctc actcagatgc tcgaaatcca ccaccggagt
     3841 caggtatccg gatttcgtca gattgttggt atctttattg atggtactga tatagccgat
     3901 caggatattg ggaagatatt tatcactgat atcggaagta actaccttat ctccctctgc
     3961 cactgcatcc tggctgtcca ccagcttgct gaaaggaatc actccctggg ccatcatctg
     4021 cagatctccg gatacgatca gattgtcacc cgtggcaagt gtcataccgc tgacattgga
     4081 attgtcggaa ataatggaag tcacccggga ccagttcggt cctacggacg taatgcgtcc
     4141 caccagaccg cctccggcga tcacattcat atcatccgcc agcccatcat tagagccttt
     4201 gtcaatgaca aaagaagaat accagttgcc agaatctctg ctgatgatcc gggcaccgat
     4261 cttattgtag tccccgtatt cttcatcaag ctccagcagc cctctcagtt cattcagttc
     4321 atatttgtcc tgctgcagca gagtgttctc ttccgtcagg gaagccacct gctccttcag
     4381 ggcactgttc tccgctaaaa gtgtccggat ctgcaccagt tcatcggaac gatttgccag
     4441 ccattctccg gccttggcga tcccctgttc aaagggtacg atcacatatc ccaccactct
     4501 gttgaacggt cggttaaata cattggttcc aaaggtcacc agcatcatga tggtgcagag
     4561 gatagttaaa ataaaaagga gatatttact cggtaaagta aacctctccc cttttctttt
     4621 tacgactggg ctcatttact catcccttca attgcataag ctaccgtttt atagttatca
     4681 tccttgatga tcttggcaag tcctgttgcc acgctggtca gcggattctc agccacattc
     4741 acatgcagtc cggtaccggt tgccagtctc tctgccagat gacttacctg ggatgctcct
     4801 ccggtcagat aaataccgtg acggtaaata tccgctgcca gttcaggggg tgttctctcc
     4861 agtatcacct ttacattatc aataatggta ttaaagtgct cctccagaga ctcgtccacc
     4921 attcttgtgg gaatctcacg ctccaccggc agaccggtga caatgtctct gccgtatact
     4981 acagcaccct ttccttcttc ttccagttcc ttcagggcaa tcttcacatt ctcagccgtc
     5041 ttgccaccga tgatcagaga gaattctctt ctgactgccg cacggatggc atcatcaaat
     5101 ttcatgccgc ccaccttgat cagacggctc agtacgatac cgcccagtga caggatggag
     5161 atttccgtgg tctcatagcc gacattcacc accaggacgc cctgggaatt tttcacatcg
     5221 atatccagac ccagaccgtc tgccacggcc ttctccacta ccatgatctt cttggccttt
     5281 acgttagctc ctttgatcag atcatagaat gctctttttt ccacctcggt aacatccgta
     5341 ggcactgcaa tgaagaaatc agaaggcttg gtattgccct tctgcagatc accgataaaa
     5401 tactttacca gcgtctccat atttttgata tccgcgatca caccattgga aagcggataa
     5461 gaaatatgaa tattgctggg agctttctca tacatttcaa atgcagagtt accataagca
     5521 aataaagtat tcttattctc gatggcaatc atattttttt cgatcatgat attgtcatca
     5581 ttgcggttat agatctttat attgttggta ccaaggtcga taccaaatgc attgtttgcc
     5641 actgtcgtta tccctttctt atatcaaaaa tttttgctct ctgaagctag tatacctgtt
     5701 atcgccaata ataatgtggt ctaacagcgg aatgtccatc tggtttccca cctttgccac
     5761 attttccgtc agctccctgt cggatctgct gggagtggga tctccgctgg gatgattgtg
     5821 cacgaggata agactgactg ccttgtcctt taacgcctcc aaaaatatct ctctgggaga
     5881 gatcagtgcc atattgacac tgccgtccga caatttcttc tcacggatca gctgtccttt
     5941 ggcatccaga cagagcagat acacacattc ggtatccctg tgtctgagct tttccataaa
     6001 atattccgct acatgatccg ctctggtgaa gcagatcccc tctctggctt tggcccggct
     6061 catcctgttg gacagttcgg cgatgcattt tagtttcacc gctttcacca tgccgatgcc
     6121 ccggatctcc atcagttctt ccacactcac atcatgcaga cccagcagtc cttctctcgg
     6181 ataggtggcc agtgccagta ccttctcagc cagcgccacc gcggaatcct ccttcgtccc
     6241 ggttcgcaga atcaccgcaa gcaattccgc ctccgtaaga ttctccggtc cgaactgcag
     6301 aaatctctca tagggaagtc cccggtctgt atttacctgt acattcttct cgggcataag
     6361 tctccttctg cctgctccga cgtatgctgc ctaacggata aaacggtata ccacgaacgc
     6421 tatgatcaca ccgatcacac tggcgatcgt gatgttaaac tccagcccga aagtcagaat
     6481 aatgaaaccg agattcagtt ccactggact gctgaggccg aaattctgtc cgtaattcag
     6541 aaaagtaccg tcgaaataag tccctataaa tctgccgatc acaaatccga tcagtaccag
     6601 gacgatcaaa acccaattaa ttctcttcaa ggtagttcct cttctttctt ctgtgtactc
     6661 tccatggctg aacagtctcc ccgtgagcct gtcttccata cgtcttttta tactatcaca
     6721 aaaagcttct tctgtacaca aatttagtag attttatttt gcgctttttc tacaggattc
     6781 gtatttttca atgtagttct acattgcttt ccggatctgt tcttccgtca caagaccttt
     6841 ttgtaccagt acctgcaggg atttcagaat accgaatttg gcatgctgcc aggtcagtcc
     6901 gccctggaaa tacactgcat acggaggctt gatggggcca tccgcagaca gctcaatgga
     6961 ggagccggaa acaaatgctc ccgctgccat aatgacctgt gcatcatatc ccggcatatc
     7021 ccagggttcc ggtgtcacat gactgtctac cggtgccgcc gcctgaattc cctggcagaa
     7081 gccgataacc ccctcaggct gaccgaaggt cactgcctga atgatatcgt gtcggctctc
     7141 actgccgttc ggtactaccg caaaacctaa tttttcatag acattggctg caaagatcgc
     7201 acccttcagt gctcccgccg tcaccgtggg agccaggaaa aatccctgat aaaaatcttt
     7261 caaaatgcca agggaagcgc cgacttcctt gcccagtccc ggcgaggtca gacggtatgc
     7321 cgcattttcc acacattctt tctttccagc aatatatccg ccgataggag ccagtccgcc
     7381 tccggggttc ttgatgagag atcctacgat catatccgca cccacgtcgg aaggttctat
     7441 ggtctccacg aattctccgt agcagttatc caccatgcag atcacgtccg gcttaatacc
     7501 tttaatgaag gcgatcagtt cgccgatccg ctgtacggac agggtgggtc tggtctggta
     7561 gcccttggat cgctgaatcg ttaccagatg agttttctca ttgatattct cacggatttt
     7621 atcgtaatca aagcttccgt ccggcagcag atccacctgg cgataagtca caccgtattc
     7681 tgccagcgat cccttcgacg ggcggatacc gatgacttct tccagcgtat catagggttt
     7741 gcctaccggg gacaacagtt cgtctccagg tcttaaattg ctcatcagag ccaatgccag
     7801 tgcatgcgtg ccgcaggtga tctgagggcg caccagtgca tcctccgtat ggaaagtcgc
     7861 tgcatagaca gcctccaggg tatctcttcc aagatcgttg tagccgtaac cggtagtgcc
     7921 caacaggcag gcctcactga cctgatgctg ctgcatggca tttaccacct ttaactgatt
     7981 gtattccgcg ttactatcca cttcatcaaa gcggtctctt aaacttttct caatggtgca
     8041 gccgtaatca tatacggtct tgctgattcc catagacagg tagatctctt cattactgct
     8101 tctcataaat ctctttttct cctaattctc tgatcatgta ttccaatatt ttctcttcgt
     8161 tataatcaaa ccgacccttg tcgatccagg tgacttcact ctctctgcga aaccatgtca
     8221 gctgtctctt ggcaaaatgc ctggtatccc gtttcaaggt atagaccgca tcttccaggc
     8281 tgcattctcc atccagatat tccagaatct ccttgtagcc cagtccctgc atggacgtca
     8341 tgcccttacg gcatcccata tccttcagtt gtcttacctc atccaccaga ccctgtgaca
     8401 tcatttcatc gattctgtcc tcgatccgtg catacagctt ttcccggtcg tccgtcagca
     8461 caaaatagct gaaattatag ggacttgtcc gttctttttc cctctcgttg tgtacagaga
     8521 tcggttctcc tgtcagttca taatattcta aagcacggat ggttctcttg atattattgg
     8581 catgaatatt ctccgccgat acgggatcga ccttcttcag catctcatgc agactctccg
     8641 gtccctgttc ttctgccaga agttccagtt tttttcgata ttcggtgttc tcttcgtttt
     8701 ccgtaaaatc aatgccccga agcaccgcct gaatgtaaaa tccggtgcct cccaccagaa
     8761 tagggatatg gctcctgtca tatatctgct ccatacattc cagacaatgt ttctggaaca
     8821 ctactacatt gaattcttcc cagggctcca gaatatcgat cagatgatgg ggcaccccct
     8881 ccatctcctc cggacggatc ttggcagaac cgatatccat atgtttatat acctgcatgg
     8941 aatctgccga caggatttct ccgtttatgg cttttgccag cgcgatggac agttttgtct
     9001 ttcccacagc tgtcggcccg gtcaggacca ccagtggttt ctttttattc tgtattaccg
     9061 acattatttt ttccttactc tttttatcct gctatccgtt gttctgggta cagtttgctg
     9121 catcgatggc gatgaccagc atcagaccct ccagttcatc ctgtggatcg gagaaattaa
     9181 tagtatacgt atctccccag tgaaacagtt ctttagagat atggatgata ctgctgcatc
     9241 cattgtatac atcataatcc catcccagaa aatctccctc cacgcgccag ccgttataat
     9301 caatctcgta tctgggcttg aaaaaagaaa actgtttgcg gatctcgcct ttcacctgcc
     9361 cgccgatttc gatttcaaat gccggaagca aggtaaacag ccgctgcctg ataactccca
     9421 gttcacgatc atttctgtca tatatgtgta actggtgtcc cagggagaaa aattccgcct
     9481 ttacaaagta ttttgcattt tcgttctcat catagacatc ataagaatct gtccaggaa
//
LOCUS       DAWDHD010000090         9497 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_432_length_9497_cov_10.879792, whole genome shotgun sequence.
ACCESSION   DAWDHD010000090 DAWDHD010000000
VERSION     DAWDHD010000090.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 9497)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9497)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9497
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_432_length_9497_cov_10.879792"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2544 a         1893 c         2739 g         2321 t
ORIGIN      
        1 gacaaaagcg atttccaagc agatcatgcc ggtgtacgat atacccatga tctattatcc
       61 cctgtcaaca ctgatgctgg cagatatcag agagatattg atcatctcca ccccaagaga
      121 ccttccggta tttcaggaat tactgggaga cggcagccag ctgggcatgc gcctggaata
      181 tgcggtgcag gatcagcccc gcgggctggc agatgccttt atcatcggtg cggatttcat
      241 cggtaatgac agagtggcac tggtactggg agataatatt ttctacggac agagcttttc
      301 cgcagtattg cgcagagtag ctgccagaga aaagggcgca actattttcg gctattatgt
      361 gcgtgatccc agagaatacg gtgtagtgga atttgatgag aacggcaagg cactttccat
      421 cgaggagaag ccggagcatc ccaagagcaa ttatgcggtt cccggactgt atttctatga
      481 caatgctgta gtggagatcg caaagaatgt aaagccctct gcaagaggag agattgagat
      541 caccagcatc aacaacgagt acctgaacag aggcagttta caggtagaga cactgggaag
      601 aggctttgca tggctggata ccggcaatca tgatgccctt ctggatgcgg cagattttgt
      661 ggcggctttc cagaagagac agggtctgta catttcctgt attgaagaga tcgctttcaa
      721 gcgaggattc attgacaaag agcagctgct tgctctggca gagccgctgc ttaagacgaa
      781 ctacggaaaa tatctggtag aggtagcaaa tggactctaa gctgcggaaa atggcgattc
      841 tggcctccat ggcggtgatc ctgctggtgg cactgctggt catgtatgtc aacagggaac
      901 agtttacggc agcttccgga ccgccggcat ccggcactca gaatgccgga gcgggtgaca
      961 gtgcatctac ggcggaaaat ggcgatcagg cagaagagac ggtctcaccg gatggtcaga
     1021 tcggcaatga tctgaaggca tttttaaaag ataatacgtt ttttgatcag gaagtaaatc
     1081 caattctgga agctgcaaag gataacagca accgactgtc tctggtagcc acctccatag
     1141 aaaaggatct gcggatccag atcgtggaca acgagggaag tccggtgacc ggggagagct
     1201 tctacgtacg actggacgga ttgggagatt acaaggatct ggatcaggac ggtatcattt
     1261 acattggaga tctggattcc ggagactatt atattgagct ccttcccatt gagggatata
     1321 aggttccggt cagtgagaca agagttcacg tcaaggataa agtggagtat ctggccattg
     1381 acgatatttc cctgttgatc aagacggagg atgaagtgga tgcagaagcc gaggacagcg
     1441 ccgtggcaga tgctctggca gatgcggaca agacagagat ccagaagctg cagaccacgt
     1501 ccggtaatgc aaaggtcggt atcgatgtct ccaaatggaa caaggagatc gactgggata
     1561 aggtgaaaaa tgctggagtg cagtttgcca ttatccgtgc cggataccgc ggttcagtaa
     1621 ccggaagcct ggtggaagat cccatgttcg tgacgaacat gaagggtgca caggccgcag
     1681 gaattccggt gggcgtatac tttttcaccc aggctgtgga tgagaaggaa gctgtggaag
     1741 aggcttccgc ggtgatcgaa ctgatccggg attaccggct gaactatccg gtatttatcg
     1801 ataccgaagg tgcgggcggt aacggccgtg cggacagtct ggatgctgag accagaacac
     1861 tggtatgtga agcgttctgc aggaccatag aaaatgccgg atacacggcg ggtgtctatg
     1921 ccagccgtaa ctggtacaac aataatctgc agacagcaag actggaaaac tatcatatct
     1981 ggctggcaga gtatcgcagt gttccgttat atcagggata ttataagacc tggcagtata
     2041 cctccaaggg aaaagtggac ggcatcgaag gtcgtgtgga catgaatatc acctacgaat
     2101 agtcaataaa aataaagagt gaggacgaaa taaatgggta aaataacagt agaaacctgt
     2161 gaaattgaag gattaaagat cattactcct acggtattcg gagatgccag aggatatttt
     2221 atggagactt acaattataa cgatttcaag gctgcgggga ttcctgaggt gttcgtacag
     2281 gacaatcagt ccgcttccaa gaagggtgta ttgcgtggac tgcatttcca gaaacatttt
     2341 cctcaggaca agctggtgcg cgtgatccgc ggagaagtct atgacgtggc agtggatctg
     2401 cgggaaggtt ccaggacttt cggccagtgg tacggagtgc ttttgtctga agagaacaag
     2461 aagcagttct ttatcccgaa gaattttgca catggttttc tggtactgtc ggattatgcg
     2521 gaattctgct ataaatgtac ggatttttat catcccgatg atgagggcgg tattgcttac
     2581 aataccccgg atatcggtgt aaaatggccg attcccgaag gtatggaact gacgctttcc
     2641 gagaaggata ccaagtgggg cacactggaa cagtacgtga aggaaaaaga gaatgaaggt
     2701 aagtaagaaa acgggtattg ccctgtttgt gacagcggca gtgatcatgg cggtgctcat
     2761 tgtattccat aagaatccgg gacctgcggt agatccggga caggaactgg ccaagaagat
     2821 catttcctgt gtggtgatcg cagcggcatg ctttgctttt attcactggt atgataaatt
     2881 caccggactg ccggtggagc tgttccagaa ccggcatctg atctggaaac tggccaaaaa
     2941 tgatttcaaa aaaagatatg cgggatctta tctgggtgcg gtatgggcta tggcacagcc
     3001 ggtggtcacg gtggctatgt attacgttgt ttttgataag atcatgggta atacgagtac
     3061 tcccctgcgg gagggtgtgg aagtaccctt tgtattgttc ctgacggcgg gactggtacc
     3121 ttggttctat tttagtgagg cattaaacaa tggaaccaat gcgctgctgg aatataacta
     3181 tctggtgaaa aaagtagtat ttaagatcag tatcctgcct atcatcaaga ttattgcagc
     3241 cacttttatt catgtatttt ttgtgtgtct gctgttgatc gtggcagcaa tttacgggta
     3301 ttatcctacc atatatacga tacagatcgt atactatagc ttctgcctgt tcatttttgt
     3361 actggcactc agctatacga cttgtgcagt agtagtattt ttccgggatc tgtctcagat
     3421 catcagtatc ggattgcaga tcggcatgtg ggccactcct attttgtgga atctggattc
     3481 cctgtccggc aattggatca cgattctgaa gctgaatccg ctggtgtata ttgtaaacgg
     3541 ttatcgaagt gctatttatg agaaggaatg gtttttccag gatttctttt ccaccatgta
     3601 tttctggatc gtaacggtag tactgttcgg gttgggagcc gttattttca aacgattgaa
     3661 ggtacatttc gcagacgtgc tataatacga atgattgtta cgatttcgag agttcgaaga
     3721 acggagaaat cgtattataa aaaacagagc atagcaaaac ttttatagat gaagaacctt
     3781 gtggggagga gctgcattac aaaactgttg tggggaaaac agaaagggtg aaagtaattg
     3841 aagggtgctc gtgaaaaatt tagaaaattt tggttatgga agccgggaaa gatcctgctg
     3901 gccgtgggat gctgcggact gctgctgttg acgctgattc cattgtttcg tctgacagtc
     3961 tatgcggttc cgtattatga tgattacaat ttcggaagat ttgccagagc tgccatggag
     4021 caggaacagt caaaatgggc tgccatctcc ggtgcactgg attgctccag aacgcagtgg
     4081 tatgcgtggc agggaaccta ttcttctatt ttctttatgg cattgatgcc ggcaatctgg
     4141 ggagaacagt attatttcct cggtacagtg ttcatcctgc tgctattgct ggcgggtacc
     4201 atgacattta cccatgtgat cctgcggaaa gtactgggca tggagaagtg gaccggcctg
     4261 gcagcacagg cagtgattac agtggcagtc tttatgttta tttattccgc acagagtggc
     4321 ttctactggt ataacggcgg agttcattac gtgggaatgc acggctttgg attgttgatg
     4381 ttgtctgtgg caatctgcct ggagagagca gaaggaagaa ctgctgccgg gctgctattt
     4441 acggcctcgg tactgctttc catgatcacg gccggaagca attttgtcac ggcattgcag
     4501 ggactcttat cgctgttgac gattctgttt ataagtgtgg tagtggaaca ccggaaaaag
     4561 ggactgtggc tgttgcctgc tacgctggta tacattatag gatttgggct gaatgtagcg
     4621 gcaccgggca attccgtgcg ggctcgcagc tatgtcggct ggggatacag tcccctggag
     4681 tccatcggca gatcttttct ggaaggagta aaacatcttc cagagtttac gggatctatt
     4741 gttctgatgg taatggtgat gctgttaccc ctgatctggc aggcacttaa ggagacggag
     4801 tatcgtttca ggtatccggg agtcgtactt ctgtggtcct tctgcctgta tgccaccggt
     4861 tacaccccaa gcctgtattc cctcggacat gccggattgt ccagaactct gaatgcggtg
     4921 aagatcacat atttattgct gttgttcctg aacgaaatat actggtgtgg ctggctgcag
     4981 caaagactgc agaagaaggc atcaggcagc acggcaagat ggctgacgaa ctggaacgga
     5041 gccgcagtgt ggtggtttta tgcgattatg ggtctggaat ttatcatgat ctttcaggtg
     5101 tcacccaatc aggcaggaca ttattcggct tatggggcct attactatgt gcataccgga
     5161 gaagcgtata atttccatca ggaatatctg gaacgggtgg agaagctatt gggagatgag
     5221 gatgatgtac agctgcaacc ttatcactac aagccctggt ttttgtgcat gggagacctc
     5281 tcggaggatg aaaataacga ggccaaccgt tctctggcca tatggtatga caaagacagt
     5341 gtaacgctga taagcgagga ttaaaattga tgcaggaaaa gaaaaagctg gccattgaag
     5401 tcgataatgt ccagaagatc tataaactat atgataagcc ctcagatcgg atgaaggagg
     5461 ctttcggctt cggaaaaaag acgaagcata agcttcacta tgccctgaag ggtgtggaca
     5521 tgaagatcta tcagggagaa acggtgggta ttatcggtac caacggttcc ggtaaatcca
     5581 cgatcctgaa gatcatcacc ggtgtattga atcccacttc gggaagagtg ctggtaaatg
     5641 gccgtatttc cgctctgctg gagctgggtg ccggatttaa tatggaatat aacggcattg
     5701 aaaatgtgta tctgaacggg acgatgatgg gattttccga gaaggagatc gaggcaaagc
     5761 ttccggagat cttaagtttt gcggatatcg gagattacgt ctatcagccg gtgaagactt
     5821 attccagcgg tatgttcgtc cggctggcct ttgcggtggc catcaatatc gagccggaga
     5881 tcctgatcgt ggatgaggct ctgagtgttg gtgacgtatt tttccaggca aaatgttatc
     5941 ataaatttga agaattcaag aagatgggca agaccattgt gttcgtcagt catgatctga
     6001 gcagtatcag caaatattgt gaccgtgtat atctgctgaa tcagggaaat atcctgggcg
     6061 aaggcagtcc caaggctatg atcgacacct acaagagggt attggtggga cagtatgacg
     6121 gcccggagag tgtgggagaa gagactgcca gtctgctgga tgatgaagac ctgcagagag
     6181 cagctgccca aaaagcagat gagaagggga ccgatgcttc ccaggcttcc atggaggcca
     6241 aggggcagaa tcccaatgcc ctggaatacg gtacgaaaca ggcacagatt gaggaatttt
     6301 acattacgga tgacagaggg gttcccacca atgcgatcct gaagggcagt atgtttacca
     6361 tccatatgcg ggttcgtttt atggatcata ttccggcccc catttttgca ttttctgtaa
     6421 aaaacgtcat cggtgtggag atcaccggta caaataccat gattgaaaag gcttttctgg
     6481 acagcgtgga gccgggagag gtgaaaaatg tgacttttac ccagaagatg aacttacagg
     6541 gtggtgagta tctgctgtct ctgggagtga ccggctacaa tcaggatacc ttcgaggtat
     6601 atcacagact gtatgatgcc ttgaatatta cggtggtatc ggacaagaac accgtaggat
     6661 attatgatat ggattccgaa acaaaagtat gggatgcgga ggagaagtaa ggaatgacag
     6721 agaccatcgg gaagatcaca ctgaatctgg ataaatatcc cggcgaggat tattattgcg
     6781 acggcagtgt ggaggacgag atcttagaca ttgtaaaaaa atacagtacg gtggaatatg
     6841 acaggatcat tgcggagcga aagagctggc cgatcctgta tcatttgtct gctctccggg
     6901 aaaatatcgt ggatttcctt cccatacaga aaacagagaa agtgctggaa gtgggcagtg
     6961 gatgcggagc catcacgggt gcactggcaa gaaaagccgg ggaagtgacc tgtgtggatc
     7021 tctcgaagaa gcgcagcctg atcaacgcct accgccacag cgaatgtgaa aatgtgacga
     7081 tccatgtggg caatttcacg gatgtggaac cggaacttcc cgcagactat gactatatct
     7141 gtctgatcgg tgtttttgag tacggacagg cttacatagg cggcaagacg ccttacgagg
     7201 atttccttaa gatcctgcag aagcatctgg caccggacgg caggatcgtg attgccattg
     7261 agaacaaata cggattgaaa tattttgccg gatgcaagga agatcatctg ggcagctggt
     7321 tttccggcat tgaaaattat ccggagggcg gtgtggtacg cacgttttcc aggaaaaagc
     7381 tggagcgcat tttcgatgcc tgcggtgtgg gagaacgcag tttttattat ccgtatcccg
     7441 attataaatt tatgaccaca gtctattccg atgcttatct tccaggacgt ggagagctgt
     7501 ccaacaatct gcggaatttt gaccgggaca gaatgctgtt gttcgatgag aagagtgctt
     7561 ttgacggaat tgtggaggaa ggattgtttt ccgttttttc caattcctat atggcggtga
     7621 tcggagcacc tctggatctg aaatatgcca gatattccaa tgaccgtgcg gaaagcttcc
     7681 ggatccgcac cgagatcctg cgggacaagg aaggctgtaa gacggtccga aaatatccgc
     7741 tgacaaaaga agcggaagcc catgtgcgtc atatgccgga ggcttatgaa aaattgaagg
     7801 aacggtatgc gggaagcagt ctggatgtca atgtctgcca tctgggagaa gagaacggca
     7861 taccttatgc ggaatttgag ttcgtgccgg gcagaccgct gtcggaactg atggacgaat
     7921 gcctggacag acaggatgtg gagggatttc acaacctgtt cgcagaatat ctggagagag
     7981 tgggctacag ggaggatgtg ccggtggcgg actttgatct gatctttgcc aatattctgg
     8041 tggatggtga ccactggaca ctgattgatt atgaatggac ttttgaccgc cccatagaga
     8101 ccagagctct ggccttccgg gctgtatact gttatgtact ggaagatgaa agacgtaatg
     8161 cattggaact ggatcgtatt ctggatcgtc tgggcatcac ggaaaacgaa gccagacagt
     8221 acagagagca ggagatggaa tttcagaaat atgtgaccgg acagaaattg tccatgggtg
     8281 agatccgcaa tctcttaggc ggcgaaattt ataagcccac ggaatggatc ggacggttcc
     8341 gtcagacgga gggagaactc agggtacaga tctatgagga caagggacag ggattttccg
     8401 aagaaaattc ttatttcccg gagaacgtct atgcagagga aaaacaggcg gaatttacgg
     8461 tgaatttcga tggaaatgtc cattatttac ggctggatcc ggccatgtgc gcctgcgtgt
     8521 gtaaaatccg ggagcttacc atgaacggac agccggttcc tgtgcaggac aaaaagatcg
     8581 ttaccaccaa tggaaaaatc ttaaagtccg cagacggtgc ggagcatccc agcgtggtat
     8641 ttcccacgga ggatccgaat ctgacgatcc gggtggatgc attggacaga aaggcagaga
     8701 acatcctgac tgtgaaaatg gagatcgtgc agattcccct tgctgtggca agtgacatgg
     8761 cgggagcggt gaaaaaattt ttctaaggag acaggaaact tgagcattcg gacgtcagta
     8821 aagcagatgc ttgtcagaca acaggataaa aagtatgaag cagaactggc aaagctgcgg
     8881 gtgacctacg cgcaatgggc tgcggagcag gaaaaaaaga ttgcggaaac agtggtcaca
     8941 gagatcgggg aaagagccgg tcttgcggaa ttcgtgatct acaggcagca gaaaggacag
     9001 ctggcggaaa atgctgtgga gcggatcaat gcgtattttg tgaagcatcc ggaggcagag
     9061 atcgtctatg gagatgaaga tctgctgtca gagaacggag agcgtgtgat cccctggttc
     9121 aagccttgct gggctccgga tacctaccgg gctttcttct atgtgggaag cgtcgtggca
     9181 gtgcggagca gactgttgca gaaactgggg gagcccgggg ctgtgacaga aggagagagc
     9241 accggcaggg aaatagtatt ttcgaaagcg gaagagatcc gtcccctgat ggaccggctg
     9301 tttctggcgg ccggtggatt tgagagaggc tgtcatacca ttggacatct ggaagaagtg
     9361 ctgtttcacg gcacttttgg tactgctgga atcggattgc agggaccggc agaaaccagc
     9421 cgggagaaag cggaagatga gcagaacccc tgggaagaga accggacggc agcggaatcc
     9481 gcaaaactgt ccgtgga
//
LOCUS       DAWDHD010000091         9438 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_435_length_9438_cov_15.821699, whole genome shotgun sequence.
ACCESSION   DAWDHD010000091 DAWDHD010000000
VERSION     DAWDHD010000091.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 9438)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9438)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9438
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_435_length_9438_cov_15.821699"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2598 a         2399 c         1805 g         2636 t
ORIGIN      
        1 aaccagtgac acgaggattt tcagtcctct gctctaccaa ctgagctatc tgggcccgcg
       61 acttaatcct acttgcaatc tcgtttcatg actgctctgc gtcacaacaa tcgtagtcta
      121 cacaaatttc gtccaaatgt caagtacctt atcaaaaaaa ttttaaattt ttgcaattat
      181 tcctctatct cctacaaatg atgcggttaa aactcctctt tttccccgta ccattgcagt
      241 aaaagctcca ccacccggtt gtagctgatc atgccatccg aaacaccatt caatttcagt
      301 gaagcattgg tcagggtatc tgaaacactg tccaccgtct ccgtattcac cacagctttt
      361 ccgttgattc tctcccattg ctcttctgtg acaaagatat tgtcctcctg cacctgctgt
      421 aatacctgta acggatgtac cgcctctctg gccgccgcat agctgtccgg atccgccagt
      481 cttgtcttat acagatcgtt tgccacataa tccagcacac ttaagtaacc cgcatattga
      541 aaagtactgt catccgactc tacgcaggcc agaaatgcaa taaaattcgc ctcatcctca
      601 taaatatagc cacggatatg agccagttca tgacacatgg ttgcaggctt tttcataata
      661 tacatgacat cattgtaatt tgcctccatg gaaaacggga aataatagcc acacatatac
      721 atctgacaca tgaaatccga gaaaaacata gccttcggcc ggggataata accatccagc
      781 tggtcatacc ttttccccag gctctgcatc acatcgatgg ctttatcctc catatccacc
      841 gcattacccc ggctgtccac accacccgag tacaccacgg caccactgtc atcccgttcc
      901 atgacctcac tgagctcatt acagtgccgg acgatatcat tgtagatccg aagcagttcc
      961 tccgttcgtt cctgtgttgt ctgctcctgc gcttcctctt caccgaaata tttctccgaa
     1021 aatgtagacc catgatagat catggtacaa ttcagcgtca taatggcaaa tacggcaagc
     1081 aacgtccatg caaaaaaacc aaaatatccc ttcactcccc ggcagtactt ttccgaatgt
     1141 ctgcacgccg gaatcagcaa actgcatccc agcacaaccg cagcaagcac caccagaatc
     1201 cctgccacaa tcatccattc gcccacggaa aagggaaaca accctgtaat ccttccgtag
     1261 gtattgaccc agattgggaa aatataatca atataagcat cgctgaggct tctgctgctc
     1321 caggcaacga cctgaagcag taaaagaact gcaacgatgc ttaaataaat tcgaaaactt
     1381 ttcttatggg atttcttatt ctccatagta ctccttaagt ggcttcctct tttcccacct
     1441 tgatcctggt cttcttccag atgccaccgc ggtacaaaac atacaggatc aacgcagaaa
     1501 tgccattact gatcaccacg gaataccaga cactgcgcac gcccatatgc aggacgctct
     1561 cgcagaacca cagtgtcagg aaacggaata cccacaatct ggtcacatcg atggccatgg
     1621 tcacctccgt atggccggtt ccctggaaca aaccggaggt cgtattgtat atgccattgg
     1681 taaagcacca gaatgccata acacttaaaa agtccgctgc catgcccacg acttccgcat
     1741 ccttagagaa gatcctgaca atagccgtgg agatcatatc tcttgataaa atcataccac
     1801 ctatgaacaa aaatacaacc gatatccaca tggaaaaacg ataccccttc tctgcacggt
     1861 ccgactgctt tgctcccatg ttctgcccca cgatggtggc cgttgccgag ccgatggcat
     1921 tggagggcag cgtgatcagt ccattcactt tgttgccgat gccataagcc gccatggctt
     1981 ccgtcccgta gacaaacacg tttttactca tcagcagaaa tcccagctgc atggtacttc
     2041 caccgatagc ggtaggcaga ccgatcttaa ggatcattcc cagtttttcc ttttgtaaac
     2101 gcatgtattt ccggttcagg cagatatcat tttcctgtct gcacagaagt ccaaaagcca
     2161 ctatggccgg aatcgctttt gccaacaatg ttgccagtgc cgctcctgca gtccccagat
     2221 tcagaatgat catgaaaaac ggatccagga acagattcag cgtgatgcca cccagattca
     2281 gaaacatagg gcgtaccgta tctccctgtg cctgatgtac tgcggaaaaa atgttgacca
     2341 tatataagaa tggcatatcc cagatgacta cacgcaggta gactcttgca taatacgcag
     2401 ttcctccgtc tgctcccagc cagttcacaa tcgccggtgt ccccagaaaa cagagcgtag
     2461 cacagaccac tgcaaatccc atggcacagg cgaaaatctg gtttgccatg gatctggcat
     2521 cctctttttc cccagcgccg atatactgcg caatgaggac tgcaccggcc acagtgatgc
     2581 cgctgccgaa attcgtaata atattctgca tcggtgtcac aagattgatc gccgccagtt
     2641 gctccgtgcc gatcttcccc agccaataag tatccgtcag gttatacatt gtctgcaaaa
     2701 agctgttgat cactacggga attgccagag acaggatcgc tctgatcaga tttccgtgca
     2761 atatcagatc cggattatat tttttagccg ttttcatcgc catcactcct aagttgttct
     2821 tatctatgtc tttccaaatc taagtcgtat tatacgtcaa tttttcagtg gtttccacgg
     2881 agattttagg gttatatgta ttaaaacact tttttattat ctttttaatt tacatcatgg
     2941 aaaggcgcat cgccagacgg caatgcagat tcttgtgcag gcacaaatca gtacttggct
     3001 catcgtatac gcaaaaaaac cctcccacag catctgcctg cggaagggat ctttttctta
     3061 attctgaagt catggtcaga cttatttgat tacaaggctg ctccaaccat cgctggggat
     3121 cagagagata taggtcttac cggtattgat ctggatctcc tcaccggtct tcgcatcata
     3181 gtaatgtgta atagcgttct cggaagactt ggtccaggta acttcgatag ccttaccctg
     3241 agtaatgtag taaccggagc gtccggagtc gatggcattg tagatcaggt agccttcacc
     3301 cagttctgca aaagtggtat cctgcagaat aacgttctcg aaagtgagct gtgcattgct
     3361 gtcagcatcc ttgtgtgcag aaccatactc atagtaatca taggttccgg tctcttcgtt
     3421 gtacttcaga gtagaaccgt tgtgcttgaa aggaagctca atggtagtag cgctgatagc
     3481 atcacttcta tcatcaaagg taacttctcc gtctgcaaat ttgaagtgag gtccctgata
     3541 gtaatcattg taagtagtgg tatacttgga atttgcaaaa cgctgcttca gaccgtcata
     3601 ggtcttgccc ttctggctgt tggtataggt atcataagta atatactcgg taaactcagt
     3661 tgccttacca ttgttgaaac gggcgaaacc accactgaag ttagcggaat aatcttttgc
     3721 tacccagtca ttgatgaagt aagggccacc atcatggcag agaactgcgt tccactcagc
     3781 tgccagcatg aagttggtag gacgtgcact acgaatacta cccagctgct cgatcttctc
     3841 ccagtccttt acgactacca tgaaacgtgt aattctgtcg ttcagagtac tgttcatgat
     3901 ctcatataca acatctgcat ctgcggtgcc gaaatgaggc agtgcggttc tctcgttatc
     3961 taccattacc gcgatgggac gctgatcctt cagatcctca tcgatccact cgttggtaag
     4021 ttcgctgcga taggagtgct ccggtgcaac ttcatccgcg gaagactcag ttgcggcagg
     4081 ctgctctacc tccttggtat cggtagcaat aggctctgct tctacggtct ggatcggaga
     4141 agagatcggc tcactgtttc cacagccggt cagaacgcct actgcaatta aaattgctgc
     4201 aagtactttc tttttcatga atgcttcctt tcatctgttt gttctatccg gtctctcgac
     4261 cggcactcta aaatacattg tatccctaaa gtcctatgtt tgtccaccga aaattcttaa
     4321 gattttcttg agattttctt tcataaaaaa tgacacctgg caattatatg ctggtgtcat
     4381 ttttgttttc tttattattc ctttacagct ccgatattta ctccctgtac aaaatatttc
     4441 tgacagaatg gataaatgat cataaacggc aaaattgcta caatgactgc tgcatttttt
     4501 actgtatttt ccgtagctcc ttctaccgca gacggcagat cacttcccat gatcaatgcc
     4561 cgcagtttca tctggaagtt atataaattt gaattcgtga tatacagctt atagttcgtg
     4621 taatcattcc aataccccac cgcatacatc agtcctatgg atgccagtgc agcctttgac
     4681 aacggcaata cgattttgta caggatccta aaatgtgaac aaccgtcaat ctctgccgat
     4741 tccatcaggc tgatcgggat tccttcaaaa aaattcttca tcagcaccag attatataca
     4801 ttgattgcag gcagcaggat cacaccccac agattatttg tcagatggaa atccttcatt
     4861 accagatagg tcgggatcat acctccgtta aatatcatgg taaacaacag cacattggca
     4921 ataaatgccc gccccggcat cttccactga atcagcacat atgccccaag tactgagatc
     4981 aacaatccca tgatcgttcc cataaccgtc gtgtaacagg aaatcaggaa tggtctgagc
     5041 atcgtcggat tggtaaaaat tgatttatac cctgcgaacc cgaacttctc cggaaacaac
     5101 ttcataccat aagccgtctt cagatcaaaa gagtccacca gtactttata taccgggata
     5161 atcatcacca gcgaaaatac tgtaaatatt acgatcaata cgatatcaaa aattttcact
     5221 ttctttttca tcatacaata cctcttcctc tcgtcttatg gcttactctg ttacatacca
     5281 gcaccagaat acagcttata atagaggaaa acataccgac cgctgtcgta tacccataat
     5341 ccggaattgc accggagtta aatgtctgcg tatagatata agtctgcaat acatccgata
     5401 ccttgataac ggcatcattc tgtaatacaa aaactgactc aaacaaattc ataaccttcg
     5461 ccagattcaa tacaagtacc gtcacgattg tattgttcaa cgcaggcagg gtaatataga
     5521 tcatcttctg gaaacggttt gccccatcca tcgacgctgc ttcatataat tccgggctga
     5581 ttcccgccag cgttgccatg aaaataatcg ttccccagcc cgtatctttc cagacattaa
     5641 ttatgtaata cgaaaatctc caccatttct ggtctccgag gaaataaata ttatccgtga
     5701 tcaagtgtaa tttcatcaga acatcattga ccaatcccgc ggatgtcgga gacagaatca
     5761 tggaaaatat ggatgctgcc actacccagg acatgaaatg cggaatgtat acaatcgtct
     5821 gaaatacctt tttcaacttt aaaaaaatca attcattcaa taataccgaa ataataactg
     5881 ccgcaccggt attcaaaatc agctttgtaa tactcagcac cagcgtattg ctgaacgcct
     5941 tccaaaaccc cggcatactg aacattttta caaaatgctt cattcctacg aagatcggat
     6001 cattgattcc tttatatgta aaaaaggcat aacggatacc aaacatagga agataataaa
     6061 atatcaatgc tgccaccaat accggtgttg ccatcagata gactccccga tatgcacggg
     6121 cgtgcttgaa cacggttcct ccaggcgtcc tcttaggcaa agttttttct ttctctttca
     6181 caacgtttac cctccgttcc ggctgctcta tgtcagctat tcaaaaatcc catatattcg
     6241 ttgtactttg tctcttctgt tttattcata tgtatggtca gtccatcctc tgagaaatag
     6301 agacggaagc ggatggatgc aacactttct ccgatcagcc agcagaagat atacagcgta
     6361 ttttgatcca tccaggctcc ggaagaagca catttctcct tgtatatagg gaactctccc
     6421 tcttccagat gtccaattcc aaagggaagc tgacattctc ttccctctat ttcatacgag
     6481 aatactcctt tttgatcctc ctcggaaaaa gttacgccac accatgtaaa tccatacttg
     6541 tttctgagca gcggatattt ttttccgaaa tctgcacaag gttttccttc aaataccgta
     6601 gataacactg gcaacgttgt tttctccaca tcgttttccg gggaatcatc cttcagcagg
     6661 acttctctca ctgcggcgag tatcatctgc tcggctccgc ccataccctg cgtatctgct
     6721 gtcgttacaa caacgagatc catttccgga actgccagca ttatctgacc gcccagcccc
     6781 aacattgcaa aagtcccctc catcgggatc cagaactgat atccgtacca gcggtcgtcg
     6841 ccatcgagct gtgttgctgt tctcggttct gtcgctctcg ctgcataaga acctttttcc
     6901 tgctttaaca gcatacagcc ggtacgaaga agatcttcgg gagtagccat caggccactc
     6961 cctcccatag aggttccaaa gggatcctct atgatataag actcctcgct gaagccaatc
     7021 tggcgcagca gcttttcttt cagataatcc aacatctttt caccggtcag tttttctacc
     7081 agcgcacaga gcgtatggga ggatgaggta tcgtacataa agagagttcc gggtctgtgg
     7141 gtgggttctg tctggaaaaa gctcctcacc cagttctcag ttgtgctggt cttgttataa
     7201 gttgtcatgt tgtaacaggt ctgcattttc aacatatttt ctatcgtcat ctctgccagc
     7261 cagggatgca ctcttccggg cagatattcc gggaaataat cacagatagg atcctgcagg
     7321 gaaatttttc cttcctgctc taataatccg attgccagtg aagtaaagct tttggtcaca
     7381 gaaaacattc tctgcagctt attacggcaa tagggtgcat aatatttttc aaacagcagg
     7441 ttcccatgtt gtgcgatcag cacactgtgt aaaggaattt ttcttttttc cacttctttt
     7501 acaaattcct gcatattctg ttccgtcagt tctctcattt tagctgccat accataacct
     7561 cccatgctgt cgccttttta tttattattc agggaatcta cgattgcctg agaccattcg
     7621 gctccgcctt cctgttcaaa tcgtttatat ccctcttcca cagaaacacc ctgtaccacg
     7681 cagtccgcaa taatagagtt cttcaagctg gtcagatcgc cgttatactg actcatcata
     7741 tctgttgtta ccggcagagg tgccgtcttg ttattggcat tgaagatttc catagattcc
     7801 tctgtatatt tctttacggg aatcagtctt tctgtcaacg ggcagatatt ggtatctccc
     7861 ataataatgg tcgtatactg tactccgggc tgagaacgca tctccagcat atggaattct
     7921 ccatcctgat atgtattacc acataccgtt tccgcctgtc tggaccaatg aacgccttct
     7981 actccataga tccacaatgt ttccatgtct ccatcatcaa aaatgctttc cagaaaatat
     8041 ttgaatacgc cttccggatt cttacaggaa gaagtaatcg catacaccgg tgcacatctc
     8101 tgccagtatt ttccaacctc tgcgataggc ggcataaata ccagttcgct gtcctgtcca
     8161 ttcgcagcca gtccatccga cagctgatca gaccatccgc ctgcccagta agtaaatgct
     8221 ccgaactcac cttccatgaa tttggtacgg cagttgctgg tcgcattcgt cagagattcc
     8281 ttatcgatat aacccttgtt gtaatgctca tgtacggtag agaagcacaa agcaaaacaa
     8341 ggatagccag ccctgccact attccgaagt caggggaacg caatagaaaa atttatagct
     8401 gtatgctata cttaattagc atttgaacat atcaagctga cacagcaatc tctttacttg
     8461 ccgttaatgc gcgtctacca atagtccgca caaagcaaga gtagcgtaaa ggtataaaaa
     8521 tttcaaactt ctttgtaacg aaattccgat ttctccgtct aacctatgaa gctgaaggag
     8581 gtgatgacaa gtatggacta tgaaaagctc tataaagcct actatttaca ggtatactcg
     8641 tatactattt ctcttgccaa aaatcgtgag atagcagagg aaatcacgca gaacacattc
     8701 tataaggctg tttctacaac atcacaattc aaaggtaagt cagatgaatt gacatggctc
     8761 tgctccatag caaaaaacct ttaccatgat gaaatgcgaa gccgtcagcg agtagctgat
     8821 gtgagtgaaa tcaatgactt gccatcaaat gaaaatgttg agaactcagt tgcagattct
     8881 gacatggcgt tccgcataca cctcgttctt caccgtttgg aagaaccata caaagaagtc
     8941 tttcagcttc gcgtattcgg ggaactatct ttttcacaaa tcgctgcaat ttttggaaaa
     9001 acagaatcat gggcgagagt tacttatcat cgtgcaaagc taaaaattca agaaaggatg
     9061 gatgaaaagc atgagtaaac aatgcgatat tgttcgagat attcttccgc tgtatgttga
     9121 cggtgcttgc agcgaagcaa gcgcagagat ggtaaaagag catttgaacg cctgtgctga
     9181 ctgtaacgca atttatcaga aattgctttc tcacacgagc gaagatgttc ttcacgaaga
     9241 aagcgaaagc gttattatgc gccatgaggc aaaagaaaag cagagaggca gaaaaaagat
     9301 aacgattgct gttctcgttt ccatcgctct ttgtaccatt gcaattttta cagctctctt
     9361 tctgttacct ataaacattg cttatgagcc tgtcaaaatc aacttcccat ttgaggttga
     9421 agatgtcgaa aacgttga
//
LOCUS       DAWDHD010000092         9413 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_437_length_9413_cov_11.454584, whole genome shotgun sequence.
ACCESSION   DAWDHD010000092 DAWDHD010000000
VERSION     DAWDHD010000092.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 9413)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9413)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9413
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_437_length_9413_cov_11.454584"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2122..2221
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2595 a         1926 c         2715 g         2077 t
ORIGIN      
        1 caaaaactat gacaattcag gaaatcaagg cacttccccg tacggaggaa ggcattttcg
       61 acctggcagc cgtgcagcag agtgcaggtc tgggcaacat ttatcaggcg gcggatctgg
      121 tgtatcccgt ctatgcggcc tatgagacca cggagaataa aaaagaaggc tatccggata
      181 tcatggcaca gatgcgtgtg ctgaaaaagc atgcagaaag tgaattcagt gctgaaaacg
      241 gtgcagctta taccgcagtc atgcttcata ccgtagagca gatcagcccg gagatctacg
      301 agaattacag agagcttctg gacaatttcc gcagtgcggt aaagcgtatg ctggagcagt
      361 attatgatgc aaaggaaaat aagtttgcca tggatgccac atcggaaaag gtattttgcg
      421 atgccgtaca gaaggcatgt gcggagtatt tattgttagc ggaaaaatat caggagtgca
      481 ttaggtagag aatgagagga aacagcgcat ggaaatcaga catattatgc atactgcccg
      541 cagtgcgacc atagagatcg cagacggcgg cagatatttt acaaaacagc ccgtggagct
      601 gcgggtaaac ggtgagccgt cactgaccac agagaagact ataacttccc tgtttagcct
      661 taagccggat cagaaatatg tggtggaagc ttatgtgaac ggagaaaagc agggcgaagt
      721 atctttcaag acggattatg agtacgtgac tctggatgtg accagattcg gtgccaaggg
      781 tgacggggaa catgacgata cccactttat tcaggctgcc atcatggcat gtccggtgaa
      841 cagcagagtg tatttcccgg cgggaatcta ccgcataacc agcctgttct taaagagcca
      901 tatccggatc gagctggcag agggtgcgga gctgaaggcc tttaccgaca gagagaaatt
      961 ccctacgttc cccggaatgc tgcagagcta tgatgagacc gacgagtaca atctgggcac
     1021 ctgggagggc aatcctttac ctatgttctc cggcattatc tgtggtatcg gtgcagagga
     1081 tgtggtgatc tatgggcagg gtaagatcaa cggcaatgct tccaaagaaa actggtggaa
     1141 ggatccgaag gttatgaata ttgcattccg tcccagactg tttttcatca gcggatgtaa
     1201 aaatgtgact ttgcagggtg tgaccttctg caattcccct tcctggaccc tgcatcccta
     1261 tttcagcaat gatctgaaat tcatcggtgt caccgtgcag aatcccagcg attcccccaa
     1321 caccgacgga ctggatccgg aatcctgcaa aaatgtggag atcctgggtg tgcgtttttc
     1381 cctgggagat gactgtattg cagtcaagag cggtaagatc tacatgggca aaaagcataa
     1441 gactccttcc gagaatatcc atatccgtca gtgccttatg gagaacggcc acggtgccgt
     1501 aactctggga agtgaaatgg caggcggcgt ggtaaatctg acggtggaag actgtgtttt
     1561 ccgtcataca gacagaggtc tgcgtatcaa gacccgtcgc ggcagaggaa aagatgcaat
     1621 tctcagcaat atcattttcc gaaatctgac gctggaccat gtgatgacac cgctggtggt
     1681 caactgcttc tatttctgtg atcccgacgg caagaccact tatgtccagt ccagagatgt
     1741 ttaccctgtg gatgacagaa caccgcaggt gaaaaagatg gtatttgaaa acatggaatg
     1801 tacgaactgc catgtggcag ctgcgttctt cgacggactt cccgagcaga agatcgagga
     1861 aatctacatg aaggacattt ccatcagcta cgctgaagat gccaagtgtg atgtcccagc
     1921 catgtctgac ggcgtggaga agagcagcaa gcgcggaatg tttgccagaa atgtagcaaa
     1981 actgacactg gacaatgtga agattgaagg acaggtcgga gaagcttacg aactggaagg
     2041 tgtggatgaa ctgatcagac gttgatataa tgagcgcaag agagccagag ggagcttgct
     2101 cactctcggc gaacggcgaa gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nataatgagc gcaagagagc cagagggagc ttgctcactc tcggcgaacg gcgaagctga
     2281 tcatgatagc tgcgaagcag gatgtagagt gcgatagcac ggaattcgtg agctcacgct
     2341 ataatagaga taagcagaag agaatacggc ccaggagccg tgttcggaat ggaggaaaat
     2401 atgcagttag gaatccgttt acatgatacg aaaaagcttc cttttgagga gcgtattgcc
     2461 aatgtacatg agttgggctt tgcctgcgga catctggctc cgggcaaggt gatcagcgag
     2521 tttcccacca cggatgaggc gctgaccccg ggacttgcca tgtacatgaa aaatgtattc
     2581 gcaaaaaatc aggtggatgt tgcggtatta ggatgctatt tgaacctggc aaaccccaac
     2641 ccggagcagc ttgcgaaaat tacccaccgt tatatggcac atatccgttt tgcttcctgg
     2701 ctgggctgcg gtgtcgtggg aactgagacc ggtgctccca acgagaccta taccgcggta
     2761 ccggaatgcc acggagaaga agcattacag acctttatca ccaatttacg cccggtagta
     2821 aaatacgcgg agcagatggg cgttgtcatg gccatcgaac cggtatggaa gcatattgta
     2881 tacaaccccg ccagagccag aagagtgctg gatgagatca attctcccaa ccttcagatc
     2941 attctggatc cggtgaacct gctggattac tgcaattaca aggatcaggt ggctatcgtg
     3001 gatgaagcaa tcgatctgct gggtgaagat gtggctatgg tacatctgaa agactttatt
     3061 cccgaggacg ggaagcttcg ttctgtaggc tgcggtctgg gacagatgga ttatacttct
     3121 gtactgaagt ttatgaagga gcgcaagccc tttatccatg ccactctgga ggacaccaca
     3181 ccggaaaata acgtgcaggt gaaaaatttt attcagggac tctatgacaa tctgtaaaga
     3241 tgtggtatac tcttccagga gtatccccag tagattagta ggagaactga aatgttaaat
     3301 gaattttcca gaacagaatt attgatcggc aaggaagcca tgcagcgcct gtacggcgca
     3361 agagttgctg tcttcggcat cggcggtgtc ggcggttatg tggtggaggc tctggcccgc
     3421 agtggtgtag gagcctttga tctgatcgat gacgataagg tctgtctgac gaatattaac
     3481 cgtcagatca tcgctaccag gaagaccatc ggcaaataca aggtagatgt catggaggaa
     3541 cgtgtacact ccatcaaccc ggaggcacag gttacagtac acaaatgttt ttatctcccg
     3601 gagacggcag accagtttga ttttacccag tatgattacg tggtggatgc ggtggatacc
     3661 gtgactgcca agctggagat catcatgcgg gcgaaggagg cgggagttcc cgttatcagc
     3721 tgtatgggag ccggcaacaa attagacccc acgcagttcc atgtggcgga tatctacaag
     3781 actaccatgt gccctttggc gaaggtcatg cgcagagaac tcaaaaagcg cggtgtgaag
     3841 aagctgaagg tggtgtattc tacggaaagc ccggtgaagc cgctggaaaa tatggaaaac
     3901 agctgcaaga caggctgtgt ctgccctccg ggtaccactc ataaatgcac ggacagaaga
     3961 gccatccccg gcagtatttc atttgtaccg tctgtagcgg gcttgattct cgcaggcgaa
     4021 gtgatcaagg atctgattgc agaaaaagaa tagctttttt attaactgtg cggtataatt
     4081 ctattatgca tgaaccgtta aaggtacaaa ggaggttgct ttatttatga cagaagaggt
     4141 aagcaaagaa cagatcaaag gggaaaatgg gacaggtatt gatgaagcta agcgcctgaa
     4201 ggagaaaaag ggagctaaca tggtagtcaa gatcctggcg atcgtactgg taccgcttat
     4261 agcgatcgca gtcattgcca tcctggcatt gaattccgca ggggacagaa tttccgacgc
     4321 gatgatgaag catgagctgg cagcgacgga atatgcactg gaaatgagcc tgaacaattc
     4381 cgctcccgga gacttttcat acgagaacgg agcactttat aaaggggaac tgaatcttac
     4441 ggacaataag caggtactcg atgctttgaa acagaatgcc ggtgtggatg tggcactgtt
     4501 ctggggcagt gatctggctg tgaccaatct gaccggaggt accatcactc tgagtgaaaa
     4561 agtggcttcc aaggtactgg gtggagaaag ctatttttcc aattccctga agctggggga
     4621 taccggatgc tatgcatatc tggcaccttt gaagaatacc gacggcacgg ttgtgggtat
     4681 tctggctgcc tgtatgccgg taagggaagt gaatgcgctt tacaaaggga ttgtaacagg
     4741 aagagcaata gcgattctga tccttgtggc agcattctgt gtctccacct ttattgtagt
     4801 aggcatgatc tccaaagcac tactgggtgt agtaggtaat ctggatcaga tcgcagaggg
     4861 taaattgaac ttcaaggttc ccgataagct gttaaaccgc agtgatgaag taggtaagag
     4921 cgcacgggct gtatactctg tagtgaacgg cttttccgga attatcaggg atattcacaa
     4981 ttccatgctg gatatgaatg agtttacggg aacgttcacc agcaattttg acagcatcgg
     5041 acagtccatt tcttctgtaa atacagctgt gaatgagatt gcccagggag ctaccacaca
     5101 ggcggcggat acccagaaag tcagcgaaag catgaacgag atgagcaatg cactgggccg
     5161 tacggcagac agtgttaatg ctttgagcag cagtgctgcc aacatgaagg agagcaatgc
     5221 taccgtggac agcaccctga aggaactgtt ggagatcagc tctcatacac agcagtcggt
     5281 ggatcaggtg caggagcaga ccaatatcac caacgagtcc gcacaggcga ttcaggcagc
     5341 taccgatatc atcgcaggta ttgcgaacca gaccaacctg ctgtcattaa atgccagtat
     5401 tgaggcagcg agagccggtg agatgggcag aggctttgca gtcgtagcgg aagagatccg
     5461 tggtctggca gaccagtcca gggaatccgc agacaagatc cgcagcatcg tggagaacct
     5521 gatcagcaat tccaaccaga gcgttcagat catgaacggt gtggtaggtg agatccatca
     5581 gcagaacgag aagctgggta ccacgctgaa tgtcttcagc accctgaacc aggaggttca
     5641 gaaggtagtc ggtgagatca atgtgatctc cggcgagctg gatcatattg agaattataa
     5701 gacggatgtg gtggagaaga tcgacggact taccgagatc tcccagaaca atgcggcaag
     5761 caccgaagag accgcagcta ccatggatca gctggcggag atcgtggagg attgccgaca
     5821 ggctaccaca cagctgaatg aaattgcggg ttcactgaat gcaaatgcga agaaattcca
     5881 gctcggataa gatggaacgg agcaaatgga gaaatgaaga ggggcactgt tacagaaaaa
     5941 actgtggcag tgcccttttt ggaaaggaga cagccaatgg aggataccgc aatattggaa
     6001 ctgtattggg cgagggacga acaggccatc gcagagacac aaaagagcta cggaaaatac
     6061 tgctatagta ttgcttatca tatcctgcat gacagagaag ataccgaaga atgtctgaat
     6121 gatacctgga tgcgggcttg gaatgcgatt ccaccgaaaa aaccgaatcg tctggagctg
     6181 tttctgggaa ctattacccg gaacttgtcg tttgatagat ggaaggcgaa aaatgcacag
     6241 aaacgtggga atggtattat ggatacgaca ctggatgaac tggcagagtg cattccggcg
     6301 gcgcataaca cagaagaagc cgtggaagcg gcggaactgg agcggagcat caatgcgttc
     6361 ctgcatacac tttcggaaca ggagtgcaat gtattcctac gcagatactg gttcgtagag
     6421 gaatatgcgg agatcgccgg gcgttatgga atgaatctga atacggtgaa gacatcgctg
     6481 ttccggacca gaaaaaaact gcaaaaatat ctggaacaac agggaatcgt cttatagacg
     6541 gagaaagaga cgaaagcggt gatgaaggat atgagcgatt gtaataagaa caataatcaa
     6601 gatgtgaatt taaaagaaga aaaactgttc cgggcgatgt ctggagtgga tgaggatctg
     6661 ttgcttcgaa gcggacagat cagaaattcg ggaagggtac ataagttccc gatgcgctat
     6721 gtgacccgca ttgcggcagc ctgcctgtgt ttcgtggtag cgggagtatt gtatatgaca
     6781 atgagcagta ccaaaatggc agacaatacc ggcaatgcgg atacggcagc accacagatg
     6841 gcctacggta tcactgagga tcaggagatc caggaagagg cggtggatgc tgcccggtcg
     6901 ggggatacag caggaacaga gaacgagatc cagaaagagg cggcaatggc ggaagaggag
     6961 gctgctatgc cggagggtga ggctgcggag gatagtgcca atatggctga aatgacagac
     7021 tctgcttcga cagacagcgg acagcaggca gcatccggga acagtgcgga tgggcagaaa
     7081 cagaacagcc tggagagcgc aaaacaggaa agcgcaccat ccgtcagcgg caatgaagca
     7141 gtgcaggaca gtacagaggc ggcctcagaa gaggatggaa ttcttaaaaa gaaggaaaga
     7201 caattaccga agaaaatgac agtgaaagga caataaagca atgagtacga tagagaaaca
     7261 tgacatgacc cgggggaaga tattgcttcc cttagtatcc ttcacaattc cattggtact
     7321 gggtaatctg tttcagctga cctacaacgc tgcggactcc gtgatcgtag gaaaatatgt
     7381 gggagagcag gcactggcag cagtgggaac ttccacaccg attatgaata tagcgatcct
     7441 gttgatcagc gggatgtgca tgggagccag tgtgttgatg agctcccagt acggagcgaa
     7501 ggattatgat actctgtcga agcagatcag caccaccatg ctggcaggtt gtggattctc
     7561 tgtggtattt tcattgctca tgctgctgct tgcgacaccg gtgcttcggc tgatccgggt
     7621 gccggagact gcgattccgg aagccgcggt atatcttcgg atcattttcc tgggactgat
     7681 atttacattt atctataatt tcctggcgaa caccatgcgg gcgctgggag acagtaagac
     7741 accgttgtat tttctagtaa ccagtgcttt tttaaacatt ttcggagatc tgttcttcgt
     7801 ggtggtatta cgatggggcg tggcgggaag tgccattgcc acagtgtgca gcgagggtct
     7861 gtgctgcctg ctgtgcggca tttatatcaa gaaaaagatt ccactgttat gccttgggaa
     7921 aaagtggtgg gtattcgata agagcctgct gggtaagaca gtcagctacg gcagtaccag
     7981 tgccatgcag caggtatgtc tgcagctggg aaaactgatc atacagtctg tggtcaatac
     8041 gcagggtgtt gcggtcatgg cggcatttac cgcggtgaac cgagtggatg attttgccta
     8101 tacaccacag cagaatatcg gacatgccat gacgactttt ctggcacaga acaagggtgc
     8161 agggcataag gagcggatga agaagggatt ccagacaggt cttttgatcg aactggtcta
     8221 ttccgtcgga ttgttctgtg taatctgggg actggctccg cagatcatga cattgttcgc
     8281 cgaggacggg gatgcacagg tggtatctct gggcatcagt tatctgcatc tgatctcctg
     8341 gatgtatatt ctgccgggat tgaccaatgg tgtgcaggga ttcttccgtg gtattggtga
     8401 tctgaaagtg acattgtaca gcacatttat gaatatgttc ggcagagttg tggcagtatt
     8461 tgtaatgatc cgcctgttac agatggaatt tgccagcctg gcctgggcca atatggtcgg
     8521 atggatcgtg atgctgttgt tcgaggtgcc gctgctagtg aagtctatgc ggaacggcga
     8581 gtgcggatag aatgagcatg tggcggacgg tcgcgggaga cctcggaaaa tgccgcagag
     8641 tcttggcaat tgggggcgta aagcagtgtc gaaagtcggg gagcggatag agttaaggat
     8701 aggaaatttt acatgaagta tggaaagatc gtagagggaa aattcattga ccgtcccaac
     8761 cggtttattg cccatgtgga gattgcaggg aaagcggaga cggtgcatgt gaaaaatacc
     8821 ggaagatgca gggaactttt gctccccggg gcagcggtgc gtctggaaca gtcggataat
     8881 ccggcaagaa agacattgta tgacctggtg gctgtttata aagacaggat cggttggatc
     8941 aacattgaca gtcaggcatc gaataaggtc gtgaaggaat ggctggaggc gcaggactat
     9001 gatgtgatca gaccggagta tacctatgga aattcccgca ttgatttcta tctggaaaaa
     9061 ggggagcaca aaatattcat ggaagtgaaa ggctgtactc tggagatcaa cggcatcggc
     9121 tattttcccg atgcacccac ggagcgtggg gtgaagcatc tgcgggaact ggccgcagcg
     9181 agacaggaag gatatgaatg tgtggttgcc tttgtgattc agatggaagg agtgacggaa
     9241 gtacgtcctt atgtggaaat gcagccggaa tttggtaccg ctctggcgga agccgaggcc
     9301 gctggggtga aggtgttgtt tttgccatgc aaggtgggta gagactggct gaagatagat
     9361 ggaaagttac gcgagcaatg agccgcgcat aggcatagtg aagacagcga tgg
//
LOCUS       DAWDHD010000093         9297 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_441_length_9297_cov_9.495997, whole genome shotgun sequence.
ACCESSION   DAWDHD010000093 DAWDHD010000000
VERSION     DAWDHD010000093.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 9297)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9297)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9297
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_441_length_9297_cov_9.495997"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2256 a         1945 c         2845 g         2251 t
ORIGIN      
        1 aaattgttgg gagcggagtc ttcgacactt tttgtcggat acttttcttg atgcctatta
       61 tatattacca cagtatttcg gaatccggct gccgggcttt gatctgacgg cacagagaat
      121 gttcataacg attctgtgtc tctttctgct gagcaaagaa tcccggaaaa agatgttctg
      181 ggaaggggtg agaagctgca gattgtggct ttttatggca ggatatctgg gaatctgttt
      241 ctatacggcg gtactgcgtt ttcacatagg tacttttctt tatccgttca ttgaattatt
      301 gggagctttt ctggtgatct acgtactgcg ggagtatctg ggaagtgatg gatttctggt
      361 gcttttccgg cggatgcttt tgtttctgtg tgtgttggga ctcgtggagg cggtcatgag
      421 aaagacaccc tttgcctatc tggagaccat caggggactt tataccggag gcatgatccg
      481 ctccggttcc taccggatca tgggaccggc caatcattct ctggcgtatg ggctgatctt
      541 gatctccggg ttaccattat tgtgcattga tgaggagaaa cattgcttca cgctgtttcg
      601 gcatctgcct ctcctgctat tggtggtggt taatgtattt cttacgggat cccgttccac
      661 gctggcggtc atgggactgg agcttttgct gttgaccgtg ctggcaccgg gagagcagag
      721 gaagaaactg ttatttacca tagtaccggc cgggcttttg attctgcttc ttgcattttt
      781 attccccaat tctccgccgg gacattatat tctgttgcag cttagtacgg tgttggatga
      841 gatgtttgga acctcctatg ccatccgtta cggggtggac agcacctccc ttgccaacag
      901 cgcttcctac agggatgccc tgctggatat tttcacggtg gactggctga acccctggct
      961 ggggcagggc tccggatatc atttctcctg gtatattccg ggaggggcca cgatccactc
     1021 cattgacaat ttctatgtgg ccaattatat ccgctacggg tatccgggac tggtggtcta
     1081 cggactgttg atcctgcagg cggtggggga gatggtgcga tgcgggattc aaaagaagga
     1141 cagaacaatg ttaagtctgg cgcttgcggt ctgcgggtac ttcttaaatc tgtggtggat
     1201 ggatacgctg cagaccatca agtatgcgta tcttcccatc gcactgttcc aggtgatctc
     1261 ccgggaaaaa tgggaaaata tgtcggattt gcaacacaaa gcggtgcaca gatacataag
     1321 gtaaatagag agaagaaacg aaaaaaggaa acgaaaatgc gaaaaagttt ttggctgttt
     1381 gcaatagttc tgagtatgag tctggggata cgggcgcagg cggcacccag agagatcccg
     1441 gatcgtttca atactggtgt ggatacagat acagagctga cggagatccc ggaaccgggg
     1501 atttacggtg gaatccagta caagaccgga gccggaggac agctggcact ggatctggtc
     1561 tacagcaaca gacaggcggg gaatgagata gagatccgga atatggattt ttcccggtat
     1621 gcactgttga tggtcaatga gggcaaaatt gccgtggaga ccacggtgac tttccggaac
     1681 tgtaaattcg atgcagtcac taccgggcgg gaggatgccc ggatatccta tgtgttcgaa
     1741 gactgcaccc tgcgtaattt tcagggaagc aatgccacgt ttacccggtg ccgtctgggg
     1801 ggcagcagcg gagatgcgtt gaatccctat cgcaatgtga cactgagaga ttgctacatt
     1861 gcggatctgg cacatccgga cagcggggat acagtacatt ctgacggggt acagatcttc
     1921 ggatatcctt ctcttacggc ggagaatatt tccttcgaca actgcagatt cgaagtaccg
     1981 gcaatccccg gaggggggag ctatgtgaat gcctgtctca tgattgcccc ggagaagagc
     2041 ggcgccaaag gaatctattt cacaaactgc attctcaacg gaggcggtta tgccatctac
     2101 acactggtaa aagacggttt ttctctggag aatgtggtgc tgtgtaatat ctcggtaggg
     2161 gatgcggccc gttatggaac cttctaccat cgcaatgtca cggcgggggt caccatggag
     2221 aatatttata cgacggatac tttgtatgta ggcagcagat tcgtggatga agagggcagg
     2281 atccatttca gcgtgaccaa cgatacggcg caggaacgga agctgatgat cgtgacgccc
     2341 agacagacca taatactgcc tgttaaggcg tgtcagccct atgaggacag aacggcggat
     2401 atgacctttg aggattttcc ctttgatatc gatgtggtgg tgccgggggc acagtgggcg
     2461 gtatgctatg acatcacgga cgggtgcaca cagatcgcac tgtatgcgga aaggaagaga
     2521 tgattatgac ggaagtactg tatatatgcc tgtggtacaa agataaaaag agaacctggt
     2581 ccggaacggc ctggtccctg cgacaggcca tggagcctta tctgcaggtg caggatctgg
     2641 cactttcgga tcctctgtac cgcaaaatac tccggagact gcagaggaag aagggactga
     2701 accccgggcc ggtcctgcgg gaccggctgc ggctgtcggg gagatatgct gcgtggagac
     2761 atcggcacag gacagacgaa aaaatgcctg tgtttcagtt tggcggctgg ccgtcgggag
     2821 cgcatttggc atcctatctc tatcaggacc tttccatttc cgcgattctg tggctgcaga
     2881 gggaagatcc ggtgggctat cggtactgcg agtatgatca ggtgccgcaa aagacgctgg
     2941 aacgcagggc ggcgagacaa ttggagcaat tccggcaaag tgaaggggta ttcaccatga
     3001 gcaggtggct ttcggagttc ctgatccggg aatgcaggct cccccgggaa aaggtccatg
     3061 cggtgggagg cggcatcaat gtggatgctg cagggatccg tccgggatca aaggaggggc
     3121 ggcgtatcct gtttgtggga aggaattttg tgagaaaagg tggtccgttg gtggtacagg
     3181 ctttcacaat cctgcggaaa aaatatctgt ggtcagcgga gctgtatctg gcgggggcaa
     3241 aggagtcgga catccgggac gctctgcggg agaggcagat cagggtagaa gaggcggact
     3301 ggcagaagat ccacctgctg ggagatctgg ggtctgaaaa actgacagat tattataacc
     3361 tgtgtgatgt cttctgtatg ccgtcttatt ttgaagccta tggactggta tttgcagagg
     3421 cacttactta tggtctgccc tgtatcggca gagataaatt cgctatgtcg gagttcattg
     3481 aggacggatg caccggcagg cttatttccg gggaggatgc ggaggaactg gcactggatc
     3541 tgtgggaagt acttcaggat ccgaaatacc gggaggaagt ggaacggcgg agggaatggt
     3601 atctgagaga gtattcctgg gacaaggtgg cgcagagaat atgcagtgtg atggagaaaa
     3661 atgagagaga caaagatcag tatcgtagtg ccggtgtata acgcggaaaa agaattacag
     3721 cgctgtgtag acagtatttt ccggcagagc ttttccaatt gggaactgct gcttatcgat
     3781 gacggcagca gggatagctc ttcggccttg tgtgacaggc tgggggcgca ggacacaagg
     3841 gtccgggtct ggcacaagga aaacggcggt gtcagcagtg ccagaaatct ggggatctgt
     3901 aaggctgaag gagagtatct tttttttacg gacagtgatg ataccctgtt cccggatacc
     3961 ctggcaactt tatatcaaaa ggcaaaagta tccggcgcgg atctttcggt gtgcggtttt
     4021 acatatctgg tggaacagac gaaagaatgt gtggataacc ttccggaaaa agctttctgc
     4081 ggtggaggaa aagaatatct ggaggagcgg tttttatcgg acttccggcg ggaatttttc
     4141 aatccgccat ggaataagct gatccgcagg gaattgcttc tggaaaatga tatccgcttc
     4201 ggagaggaat ttgccatctg tgaggatatg gctttttcca tgcaggtgct ggaaaagagc
     4261 aaagggatct gtgtggttcc ggagtcgttg tattgttatc actataagga agcggagaat
     4321 ctggtgaacc gttttcatga gaactattat gaggctttat tgtatttccg ggaccgcacc
     4381 tggcagtgct tccgaacgct tcaggcaaag caggaactgt atgcggaggt caatgcctgt
     4441 ttcgtgggga agagtctgat gtatctgcat aagatctata gagattccgg ctatgaggaa
     4501 accaggaaat atgcggaact gaaacggatc ggtggttccc tgccgctgca gaatgccctg
     4561 cgggaatacc gggccacggg aaagaggcgc ttgctgcggg agttgctgat gcattagaac
     4621 ttccggctat gggacagtct gtaccggtgg cggtaggaca ggggaaggag agaggattgg
     4681 aactgacaaa acagatcggc gtattgcgca atcgaaaaac cttggacgga agaaaagcag
     4741 acaggaacaa agtaaatctg gagtactgga attttaatga gaatctgggg gatctgctgg
     4801 caccggtggt gacgcagtgg atgttgcacc gtctgggact ggatctgtac agagaggcgg
     4861 caaagccctg ccatctgatg accattggtt ctgtgttggg actgggagtt ttcgatgcgg
     4921 tgatctgggg cagcggggtc aattccttcg cccatgtggg acgggtgact acacaaaagg
     4981 gataccggaa gctggatatc cgggcagtca gaggtccggt caccgcacag gtgctccggg
     5041 agaacggata tacctgtccg cagatctacg gagacccggc gatcctgctg cctctgattt
     5101 atcccggcag acgaccggag aaaaagaaaa aatatgtggt gatcgaccat tacatgaagc
     5161 ggaaaacgac aggagatgac agactttcca tacgaaccgg agattaccgg accttcctcg
     5221 acaaaattct ggaggcggag gtggtatact cgtcttcgtt acatgggatc attctggcgg
     5281 agagctatgg ggtcccgtcg gtattccttc ggcaggggat ggaggaagag atgctgaaat
     5341 attatgactg gtactttgcc accggcagat atgaagtgcg ctcggcaaca tcccttgcgg
     5401 aagcaaagga aacagaaccc atgaaattgc ccgaactgga agaactgagg gagggactgc
     5461 tacagagctt tccctatgat ctgtggatcg gccagaaagc cggaaggaga aagtgtgaat
     5521 gaaaaggacc cgaaatgccg tcagaaatgt gatcttcgga ggactcctga aggggtatca
     5581 gattctggtt ccttttatca tgcgcacgct gttgatccgg tatctgggaa tggaatatct
     5641 ggggctgaac agtctgttta cttccatact gcagatcctg aatctggcgg agctgggagt
     5701 agggagtgca ctgggctaca gtatgtatgc tcccatcgcg gaaggaaaga aggacgagat
     5761 ctgtgctctg ttgtcattgt atcgcagata ttaccgcctg atcggtctgg ggatctttct
     5821 ggccggaata gtgctgctgc cgtttctgcc ctcgctggtg aaaacggaca gtattccgcc
     5881 ggatgtggat ttgtatatat tgtatctgct gcatctggga gcctgtgtca tcagttactg
     5941 gctgtttgcg tataaaaaca gtctgctggc agctcatcag cgaagtgacc tggcaaataa
     6001 ggcggatctg gcggtacgga cgctgcagta tcttatacag gccgggctgc tggtgggctt
     6061 tcggaattat tactggtatc tgctggcggc actgggagca cagatcctga cgaatctgtg
     6121 taccgcactg gtggcacaga aaaattatcc ggattatgtc ccaaagggac aggtggaaga
     6181 gcagaccagg caaaaaatcc ggggcaagat caaggacctg tttacctccc gggtgggggc
     6241 ggtcattgtg aattccgcgg atactctggt catttcgaca tttttgggac tgacggtgct
     6301 ggcagtctat cagaattatt attacatcgt tacttccgtc attggtctgg taggggtgct
     6361 tttcacctcc actctagccg ggatcggcaa tagtcttctg accgagagcg gggggaaaaa
     6421 ttatgcggac ttctgtaaat ttacactgtt gatcgcctgg atcgcaggac tttgcacctg
     6481 ctgcctgctg tgtctgttgc agccctttat gaaactgtgg atcggagagg caggcatttt
     6541 gcccggacag atcgtgatct gtctgtgtgt atattatttt atctacgagt tcaaccagct
     6601 gttcaatgcc tacaaggatg ccgccgggat ctggcacaga gaccggttcc ggacactgac
     6661 aacggcagga gtgaatctgg tcctgaatct tctgctggtg aaacctatgg ggctgtacgg
     6721 agtgatcctg tctacggtag ttgctacggt catcgtagga atgccgtggc tgatacataa
     6781 tctgtttacg acgttgtttg acggagagga ccggcaaagc tatctgggga cactggtctt
     6841 ctatggaagc gtaacatttt taaactgtat tctgaccgac cgggtatgca gtcagatacc
     6901 ggtagaagga atccgggaat tattggagcg ggcactggta tgtctgcttg tgtccaacgg
     6961 catttttctg ttgtgttacg gaggccggaa ggatttccgg gccctgttgg caatggggaa
     7021 gagatttctg cctgaaatac ctcatgataa agcgacttcg ggaaatgacc gatccgcaga
     7081 ggaaaccaca aagtttgcag attacggaaa ggtgggagct ttctatgaag attgcagtag
     7141 cgggaaccgg gtatgtggga ttgtccatgg cagttctgct ggcccagaat catgaagtaa
     7201 cggcggtgga tgtgataccg gagaaggtag agttgttaca aaaagggaga tcacctatcc
     7261 gggataaaga gatcaaagaa tatctggcag gcggcaggct gcatttgcag gctaccctgg
     7321 acggagaggt ggcttaccgg gatgcggagc ttgtgatcgt ctccacaccg acgaactatg
     7381 atcccggaat tcactttttt gataccggtt ctgtggaaac ggtggtggag acagtacgga
     7441 gggtgaatcc tgacgcggtg atcgtgatca agagtacggt gccggtggga tatacagtgt
     7501 ctctgatgga aaaatatcac acggacagga tcttgttttc tccggaattt ttgcgggaag
     7561 ggcatgcact gtatgacaat ctgtatcctt cccggatcat tgtgggagca gacacaaagt
     7621 ccgttgaaat ggcggagtat gcaagacggt ttgcagagct tttgcgtgag ggtgcaaaaa
     7681 aacaggagat ccccatacgg atcatggacc ctacggaagc ggaggcggta aaactgtttg
     7741 ccaataccta tctggcgctc cgggtcagtt tttttaacga actggacact tatgcggaga
     7801 tgcggaactt aagcagccgc aatattatcg agggtgtctg cctggatccc cggatcggga
     7861 tgcagtacaa caatccctct tttggctacg gaggatactg tctgccgaag gatacgaagc
     7921 agctactggc caattatgag aacattcccc agaatctgat ccaggccatc gtgtccgcca
     7981 acagtacccg caaggatcat atcgcggaca gtattctgga gaaggcggga ttttatcagg
     8041 gaagtcaggg tagaggcggt gttccgggac aaaaggtggt cataggaatc tatcggctta
     8101 tcatgaagtc cggctccgat aatttccgtc agtccagtat ccagggcgtc atgaaacgga
     8161 tccgggccaa aggggcacag attttgttgt acgagccgga actttcggag gatgattttt
     8221 tcggaagccc ggtcatacgg gatctggaag cctttcagcg gcagtcggat gtgatcgtgg
     8281 ccaatcgtta tcacagagat ctggagaatg tccgggagaa ggtatacacc agagatattt
     8341 attacaggga ctgagtgagg gaagaaatgg atacagagaa aaaaacgggg gaacgaatag
     8401 gaatcacgct ggcactatta gcatgtgtgg gattttccgc ttttctgatc tggctgcagc
     8461 aaagccaaga gaatgacaga cagcagctga cacaacaggt acaggacagc gggcagcggg
     8521 aagaacagac ggaaggaagc gggcagatcg agatccggag tcgtgttacc cggagcaaaa
     8581 caggggatca gccggtattt tccctgccgg gagggtttta tccggaggat attacggtag
     8641 agatcgctgc accggcggga agcagtatct attatacact ggatggcacg gtgccggatc
     8701 cggagaacgg aattctctat gaggcaccgg tggagataac gaatgtctgc ggcagtccca
     8761 atgtctattc cgccatttcc acagtgtcag cctaccagga ctatgcgccg tttaatgacg
     8821 tggacaaagc ggtggtgtta caggcagtgg cagtagatgc cggaggcagg acaagtaatg
     8881 tcacctgcgc cagctatttt gtggctatgg aggccagagc aatgtaccgg gatctgccgg
     8941 tgttgtcgct gactgtggat cctgtggagc tgtttgatta ttttggtggc aattatgtca
     9001 caggtgtgga ttatgagaat gcgctggcgg cggatgacct gcggtttgat tctgccaatt
     9061 attaccgggg tggagagatg aatgcccatg tggaatactt cgaagcggac agatatctga
     9121 cttatgaggg ggaggtaatt ttaagtttgc ggaaggacgg aaatctggat tacgggcaga
     9181 aaagttttct tatgacggga gcggatccga tgccggagag cagttgtgag ctgtatgcgc
     9241 ttgtccggga cggcacgttt cttctgtacg ggggcggcac ggatcatgtg gccaaga
//
LOCUS       DAWDHD010000094         9197 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_447_length_9197_cov_10.469810, whole genome shotgun sequence.
ACCESSION   DAWDHD010000094 DAWDHD010000000
VERSION     DAWDHD010000094.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 9197)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9197)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9197
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_447_length_9197_cov_10.469810"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2601 a         1966 c         2683 g         1947 t
ORIGIN      
        1 atgttgtatc caaacgtggt ggaaaattaa ttgccaagaa ttaatttcta catatctaat
       61 caaaagggcc cggtgcatat gcgctgcagg cccttttgat ggtattggga ggagaagagc
      121 atgaataagg gaaaagaacg gaagaagcgg gaaggaaagc agaaaaaagg agtcgggatc
      181 ggagtcaaga tcgtggcact tttactgctg ctggcagttg ctgcactcag ctgtatggga
      241 gtgctggtgc agacgttgca atcggtcatt gtaaccaacg atgaaatcgt ggccggtcag
      301 gtggctgaac aggagaaaat ctcggagctt tccagacagt ttacttacat taacgggcag
      361 gtgctgaccc atgtcatgac caccaacagt gtgacaatgg aaaatttgtc ggataagatc
      421 ctgcaggaga tcactgatat ggaacaacag atgacggagt tcgaaggact gctgtctgag
      481 ggggatgccc ggagagaggc gtttgacagt gcttctgcgg aactggcaaa atacaaaaag
      541 accgtggaga gtctgctggt caccagtgca gagaataaga cgcaggccta tgtgtctgcc
      601 acatcgaatc tgccgatgtt caatgaacat attgaaagct atatgaatca gatgctggag
      661 atcaccggag agcagatggc acaggcacag ggacagatgg agcagagtgc ggccagagtg
      721 cccatgatcg tggcggtggc aagtctggta ctgcttgcag tagtcgtggt gatcatgctg
      781 ggattgcgga tctgggtcat cggtccggtg aaatatgcta cgaggcaggt ggatgaactg
      841 gtggaaggca ttcgcaataa cagaggtgac attaccagac ggatccgggt gaaatcgggg
      901 gatgaagtcg gcagattatc catggccatc aatgatctgg tggcgcagat gcagaccatc
      961 ataagggcga ttaccgagag ctgcggacat atggaggaaa aacagaccgg tattatctcc
     1021 aatgtggaaa aggtaaatgc caccgcggag gacaccaccc agaagctggg agtgatggct
     1081 ggtggcatgc agtctgtcac ggaggctatc ggaggggtgc aacaggatac cggggtgctg
     1141 gataccacgg tggagaacat gctggaggtg gcacaggatg gacgcaatta tgcggcggat
     1201 atcaaggata gggccggaaa aatgaaggca acggcggtgg cgagtaagca ggaggcaacc
     1261 caggtgatga aggagatcga cacggcaatg acggagtcca tcgccaacag caggcagatc
     1321 cacaagatca cggaactgac ggaagaaatc ctgggaattg ctggaacaac gaatctgctg
     1381 gcactgaatg cgtctatcga ggctgccaga gcaggagaag caggccgggg attcgcagtc
     1441 gtggcggagg agatccgcaa gctcgcagac agctccagag agtcagccaa taatatccag
     1501 gagataagta accgggtagt ggaaagcgtg gaagaacttt ccgaaaatgc tacaaaactt
     1561 ttggatttta tgaataccag agtcatgaga gattacgatg ctctggagga tacgggcaca
     1621 aattatcatg aggctgcgga tcatgtggat gagatgatga atgaattccg acagaagatc
     1681 gatgaactgt tgtcggtact gcagaatgtc aatagtgcta actcacagat ggaagcaacc
     1741 gtggaggaca gtacggaaaa gttggcggtg gtaaagaaca acaaccgggg attacagcag
     1801 gagatgaagg atatttccta tgcggtggat gatctgaccg gtgcagtggg acagttgaaa
     1861 gaaagtatcc gctgttttac agtggtatag acagaaaaaa taaaatgcac gggattcccg
     1921 atggggaatt accgtgcatt ttgaatgact gcagttacat tttaggaatt ttccggagcg
     1981 tgatgcagct ccagagccag ataggaattg tggtattcct tgcagtaggt gacatctttg
     2041 tcaaaccatg cgggagggac gaaagcctct gctgcttctt tacttccaaa ctccacttct
     2101 gccatgatca gaggggcgaa gggggcatca aaaacatcca gctcgatggt cagggaataa
     2161 tctgccggag gagtgggaaa gccttctttg aatccgggat gctccagagg gatcagatag
     2221 cgtcttttgg agatcacact gccgtcggct ttttcccgta gatggtaata ggattcctcg
     2281 ttcagcggaa gattgctctc atctctggcc atcatgccgc tgcctttgta agtgagataa
     2341 tattcatcat cctgttttct ggctctgacc acaggattat aattcagata tacctgctcg
     2401 atatgatgag aaggatagct ttccagattg tcgggtaagg atttcagggt aaactttcgc
     2461 tcgatttcca tagtattaat gcctcgcttc tgtaattaag acttaaaaac acttctttag
     2521 tataacatga ttttccttga aaatacagtg aaatctgcaa atatctgccc atgggaattg
     2581 aattacgctt ccaaattgtg tatactaatc ataactgtgc aaagtcagag tatatgactt
     2641 gcaaatgaga cagaaaacaa aacggaaagc gagggaaaac catgagtaag atagcgattt
     2701 ttctggcaaa tgggtttgag gaaatagaag gtctgacggt agtggatatc tgccgcagat
     2761 gtggactgac cattgatatg gtgtccatta cggaagaaaa acaggtgatg ggatcacata
     2821 agattccggt gactgcggat atgacattgt cccaggtgaa ttttgaggaa tatgattgtc
     2881 tggtattgcc cggtggcgga cagggaacga agaatctgga ggcctgtgag cccctgatgc
     2941 agcagatcga tgctttttat gcatcgggaa aatacattgc ggcaatctgt gcggctccca
     3001 gtatcttcgg acacagagga atcctgaagg gaaagagagc atgcgcctat ccctgcttcg
     3061 aggatcatct ggaaggtgcg gctgtgactg caggccccgt ggaagtggac ggtaacgtga
     3121 tcacttcccg cggaatgggg acttccatcc ccttcggact ggcgatcgcc ggtgtattct
     3181 gtggacagga taaggcagat gcctgcgccc acgctattgt atttcagccg taagcagctg
     3241 aagagaaaga cgaggacaga aaatgaaaaa agcgttaatt acaggaatta ccggacagga
     3301 cggctcttat ctggcagagt tcctgctgga aaaaggatat gaagtgcatg gactgatccg
     3361 cagatcttcc atcagcaata ccggtcgtat cgaccatctg atccgtgagg acaagatcgt
     3421 tctccatggc ggagacatga cggattccac cagcctggtg cgcatcatcg gagaagtcag
     3481 accggatgaa atctacaatc tgggcgccca gagccatgta caggtaagct ttgaggcacc
     3541 ggaatatgcc ggagatgtag atgcactggg cgtgctgcgg attctggagg ctgtgcgtat
     3601 ggtgggactg gccgagacct gccgtattta tcaggcatcc acatccgaac tctatggccg
     3661 agtggaagaa gtgcctcaga atgagaacac cccgttccat ccctacagcc cctatgcggt
     3721 ggcaaaacag tacggcttct ggatcgtgaa ggaatacaga gaagcgtata atatgttcgc
     3781 agtcaacggc attctgttca accatgaatc cgagcgccgg ggagagactt tcgttacccg
     3841 taagatcaca ctggcagccg gcagaatcgc ctgcggtatc caggataaac tctatctggg
     3901 caatctggat tccaagagag actggggcta tgcgaaggac tatgtggaat gcatgtggct
     3961 gatgctgcag catgacaagc cggaggactt cgttattgcc accggtgtac agcataccgt
     4021 aagagaattt accacactgg cattccatta tgtgggaatc gaactggaat ggcagggcag
     4081 cggtatccat gaaaaaggaa tcaacaaggc caacggcaag gtggtggtgg aggtcagcga
     4141 ggccttttac cgtcccacgg atgtggtcaa tctgtgggga gatccgacga aggcccgcac
     4201 ggtgctggga tggaatcccc agagaaccag ctacgaggaa ctgtgccgtc tgatggcaga
     4261 gcacgatctg aagctggcaa aggctgaagc ggtagtgttg aaggaaaccg cggaataaca
     4321 ggagaaataa agagaggaag tactgtaata tcatggaaaa agacgcaaaa atatatgtag
     4381 ccggccacaa gggtatggta ggaagtgcta tttgtagagc actccagaga aacggatata
     4441 ccaatatcat cacccgcagc cataaggaac tggatctgtg cagacaggat gccgtggagg
     4501 agttcttttc cagggagaag ccggactatg tgttcctggc cgcagccaag gtgggcggca
     4561 tcatggccaa cagtgaggca ctggcggatt tcatgtatga aaatatgatc ctggagatga
     4621 atgtgatcca tgcggcatgg cagaacaact gcaagaagct gatgtttttg ggcagcagct
     4681 gtatctatcc ccgtatggca ccgcagccta tgaaggagag ctgcctgttg acttctgagc
     4741 tggagaaaac caatgaggcg tatgcactgg cgaagatctc cggactgaaa tactgcgagt
     4801 tcctgaacag acagtacgga accgactata tcagtgtcat gcccacgaat ctgtacgggc
     4861 ccaacgacaa ttatcatccc acccacagcc atgtgcttcc ggcgctgatc cgccgcttcc
     4921 atgaggcaaa agtatccggc gcgaaggaag tagtctgctg gggaacagga accccactgc
     4981 gggaatttct ctatgtggat gatctggcag atgcctgcgt gtatctgatg aatacctaca
     5041 gcggcgatga gacagtgaat ctgggaaccg gcaaggaact gaccatccgg gaattgacgg
     5101 aactggtagc gaaggtagta ggttatactg gggagatcaa atgggatccc agcaagccgg
     5161 atggaactcc cagaaagctt ctggatgtca gcaaactgga aagcctgggc taccattata
     5221 cgacggagct ggaagaaggt atccgtctga cttatgagga tttcctcaat aatcccatgc
     5281 gtgcggaacg ctaatggttc cgggcacgaa cagcgcaggc agacagacgg cccgggaaag
     5341 gatgaagtga acagaatgaa tgattttcgg aagacagcac aggagatgct ggaatttata
     5401 agaataagtc ctacctgttt tcatgcagtg gccaatatcg gaaggatgct ggaggcagca
     5461 ggctttcagc agttacagga aaaggaagaa tggaaactgg aaaagggcgg acggtattat
     5521 acggagcgca atgattcttc cgttattgcg tttgtgattc cggaaaagga ggtcggcatc
     5581 aggggctttc atatggcagc agcccacagt gattccccct gctttaagat caaagagaag
     5641 ccggaattga ccgtggagga gcattatctg cgtctgaaca ccgaaaaata cggcggcatg
     5701 atcttgtcca cctggttgga cagaccactc tccgtcgctg gcagactggc tgtgaagaac
     5761 gggaacggta tagagggcag actggtgaat atcgacagag atctctgcgt gatccccaat
     5821 gtggccatcc atatgaaccg ggaaatgaac aagggtgtgg agtacaatcc ccaggtggat
     5881 ctgctgcctt tgcttgcgga cgtatcattt gacgaatatg atgcacatac tacatacgat
     5941 gcacagaccg catcggagaa tgcggaggaa cagccggaag cagtagagaa gcccacactg
     6001 gtagcgctgg cagcagaaac ggcaggcgtg gacgctgaga cgattctcgg agaagacctg
     6061 ttcctgtaca ccagacagga aggaaaaatg ataggagcaa agggagaatt cgtgttgtct
     6121 cccagactgg atgatctgca gagcgctttt gccctgacaa aagcttttac ggagagtaca
     6181 cctgcagaat atatcaatct ctgtgcagta tttgataatg aagaagtagg aagcggtacc
     6241 agacagggcg ccgattccac ttttctggaa gatactttac agcggatcac agagggactg
     6301 caggaggggc acagcaccta tctgcgctgg gtggcagaca gcttcctgat ctctgcggat
     6361 aatgcccatg cggtacatcc caaccatccc gaaaaagcag accctgccaa ccgcccgtat
     6421 ctaaacggcg gaatcgtcat taaatatcat ggcggccaga aatacaccac tgacggtatt
     6481 tcggcagcaa agatgaagga ttactgcgaa cgggcgaagg taccttacca gacctatgcc
     6541 aaccgttccg acattgcggg aggttccact ctcggcaata tttccaccgc acatgtatca
     6601 gtgtccagcg tggatatcgg actgcctcag ctggccatgc attccgctgt ggagacagca
     6661 ggtatgaagg atacggaata tgcggtccgg gcgctgaagg aattctgggg cgaatgattt
     6721 tggcagcggg aattcttaaa aaagtataaa aagcgtggaa gcacttgaaa gttctgcacg
     6781 aaaagtgcat atttaggata caggaaaaga tacaaaaaag atatgaaaga aggctgaagt
     6841 tatgtttgaa atgaaaccgg aatactatat tggaattgac atgatcgatc aggagcataa
     6901 acagttgttt gactatgcca gtgaagcata cgaactgctg caggaagagt ttacaccgga
     6961 caagtatgac aagattgatg caattctgga aaaactgcgt gattataccg taaaacattt
     7021 ttcagatgaa gaagcgtata tggaatccat ccagtacaaa aagattttta cccagaagat
     7081 tcagcatcag gaatttatca acaaactgga tgaatttatt gatcagcatg agaaagatga
     7141 agaaaatcag gatgagcaga tcatggatat tctgaattac ctgacagact ggctgatcaa
     7201 ccatatcctt catgtggatg gacagattcc gaaggaggcg taagacctga cttttccgga
     7261 atgatttccc acatgggaca taagatgtaa gaagcggaag taatggggag tgcccatgga
     7321 ccagaaactg gaaaacatcc tgaatcttgc attggagacc ccggaggaag aacggaaaca
     7381 gacggaaagt ctgaatgtcg gttacagtgc ggagagccgc agctgggagc tgatcgtaaa
     7441 atatcatggc agtctggacg gactgcggga acagaatatt gtggtggaag agctgattgc
     7501 cggatatgcg atccttacgg taccggagac actggtagat accgtatcgg agactccgga
     7561 gatcgagtat gtggagaagc ctaagcggtt ttactatcag cagacagatc cggacggcgc
     7621 atcctgcttt ccaccggtga caagaagaac gccttttctg aatggaaggg gcgttctttt
     7681 ggctgtattg gattccggga tcacctggga tctggaggtg ttccgcaagg cagatggcag
     7741 tacgaggata cggtatctat gggatcagac gataccatgg gatcagacga ttccgggaaa
     7801 gcaggccgca tcacgggagc aggagacgct gcggaatccg acattgccac agaatcagat
     7861 agcgtcggag gaaacgggcg atacgggata tgggaggaca ccggatggat ttcccatcgg
     7921 gactgaatat acggaggagg aaatcaaccg ggcactgaac agcagtgtat tggaacgtta
     7981 ccgcctaatc cccagcaggg acctgaccgg ccatggaacg gcagtggccg ggatcgccgc
     8041 cggcaggagt gcggatggtc tctataccgg ggcagccccg gaggcggaac tgatcatcgt
     8101 gaaactggga cttcccaggg aggaaggttt tccccggacc acggagatca tgagaggcgt
     8161 tacttatgcg ctgcggaaag ccagacagtt aaatatgcct cttgtcatca atctgagctt
     8221 cggcaacagc tacggttccc acgatggaag ttctctgctg gagaggtttt tggacaatgc
     8281 ttcggagatc ggcaggacgg tgatatgcgt tggcagtggc aacgaaggag ccgccagagg
     8341 acattttgcc ggcaatataa caagggacga ccgcgtggaa ctggctgtaa gcaattatga
     8401 gagaaacctg aatatacagc tatggaaaaa ttacagcgat gtgttccgga taagactgca
     8461 ggccccggga ggtgaggaag cagaattgtc cacaaatatc cagggaggaa aatacacgct
     8521 ggaattggaa cagactagaa tactggttta tctgggagag cctctgccct atgcggtggc
     8581 acaggaaatc tatctggaca tgatcccggc agagggaggc tacatcaatg cagggatctg
     8641 gacaatccgg ctggaaccgg tagtgaccgt gacaggacaa tattatctgt atctcccagc
     8701 gggaagcgga atcggggaga gtaccggatt ctaccgtgcc acaccccagg tgacactgac
     8761 cattccgtct accgcagcca aggtgatcac cgtgggagcc tatgaccagg tctatgatac
     8821 ctatgccgat ttctccggca gaggctatgc ggatagcaca agaaccattg gtgtggctgc
     8881 cgcaggactg accaagcccg atctagtggc cccaggtgtg aatatccagg ctccggatgt
     8941 gtacggaagc tttactcccg tgaccgggac ctcttttgcg acacccattg tcagcggggc
     9001 agcagccctt ttgatggaat ggggcattgt acgggggaat gatcccttcc tctacggcga
     9061 aaaagtaaaa gcatatttca ggaaaggagc cagaccactg aggggggaga ctgaatatcc
     9121 caatgaccgg gtggggtatg ggaaactgtg cgtggcctgt agcttatacc catctccgcg
     9181 cccacgagac ggagcta
//
LOCUS       DAWDHD010000095         9141 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_450_length_9141_cov_8.998349, whole genome shotgun sequence.
ACCESSION   DAWDHD010000095 DAWDHD010000000
VERSION     DAWDHD010000095.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 9141)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9141)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9141
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_450_length_9141_cov_8.998349"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2112 a         2345 c         2041 g         2643 t
ORIGIN      
        1 caccaagact tgcactggat aagccgaaat attttgcaac agacaaagca tcctccctgc
       61 cgaatttctt ccagtcctct tctgtctggc agtaaggagt gtctctgctc tcatccacat
      121 gacaatgctc gatgatggcg gagggaatgg atctggcggt actttctctt ataattccgt
      181 aatagtcatc accgttatct ttaagtttcg tcttgactcc ccgtagaaac agtcccatgt
      241 cctgcatctg ctgcatctgt tcccaaccga actgataacc gtaagcgttg taaggctgta
      301 cggaagagat ccagacttcg gatccgaaca gatcatgatc cacggaagcg ttgtaatgaa
      361 tactgaacag gaaatccgca ttccggtctg cggcaaattg tgcccggtct gccagagaca
      421 tggtcacatc atccgtatga gtcagataga catccacatt atcgtatttt gtcagttcct
      481 cgtacatggc cagcgcggtc accatggtca tttttttctc ctgtgcaggg ccttccagcg
      541 ttccctcgtt atcaccgccg tgtccgggat caatgacaat gacgacccgt tctccggtct
      601 cctgcgctct gggagccgcc tgtgcaggca gggaatttcc ataaaaacag gctgccgcaa
      661 gactgaccgc acagatcatt gtcctgacta acagcccttt ttgctttttc catactttca
      721 tttatcgtat tcctttattt ttaaaaaacc agctttcagc agaaaactgg tttttcgtac
      781 tcgttgtttg gacatgcttt acataacgtc cagattgtct tcggcatccg cagcagtatc
      841 tacgtcatcc agatcctcat ctacggggtg caactcactg cggttggact ggataatgtc
      901 acgatactgg ttcagattgc cgatcagagc atcgtaattg gcgctggcct gctgtgtgga
      961 ggaagaaata atattctcca gatgtgccag catatcatcc atgtactgca tggcagcgga
     1021 tttgacattg ttggcctcaa tagtggcatt gtccagaatc tcctgagcct gcttactggc
     1081 catggtaacg acctcgttgg cctgggcgta agcctgctgc atgatctcat gctcgttgat
     1141 cagctcgttg gtatgcacgg tagcctcgtt gatcaatgct tctgccttgg ctttggcatc
     1201 gtttaggatg gcttccttgt tgctgatgat cttctggtag cgcttgatct catccggggt
     1261 cttcatacgc agttcccgca gtaactcatc aatctcatcc ttattcacga tgatttcggt
     1321 actggacatg aacttcggct tgcaaccctc gatgtattct tctatttcat caattaactg
     1381 ttcgattctg ctgctcatga aactctcctt atttacacat cactatgctc tgaaagcttt
     1441 ttctcgcgat atttgtcgta gatcagtttc gccacaaaat ccggaacaca aggggtgata
     1501 tctccatcaa aacttgcaat ttgcttgatg gcggaagaac tgaggtaggc atattccaga
     1561 ctggtggtca aaaataccgt atcaattttg ccatgggaca acatccggtt ggtctgtgcc
     1621 atctgcagtt catattcaaa atccgtaatg gcgcgtaagc cccttacgat aacatgcaga
     1681 tctttttccc tggcataatc cactaaaagc ccactgaaac tgtcaatctg cacattaggc
     1741 agatcttttg tggcttcttc tagtatctta acacgttcct ctacagaaaa caacggtgtc
     1801 ttttctttat tgttcaaaat gctgacgatc agaacatcaa atatttcgga tgcccgcctt
     1861 atgatatcca tatgaccgtt ggtgacggga tcaaagcttc cggtgtaaac agctcttttc
     1921 ataaaaatca tgcctttcag tgttacttcc tgcgaaggaa cacatgcttg ttggttttgt
     1981 attttttctc acggatcact tcaaacccta agtcttccgc ataggagaaa tcggtatgca
     2041 gagaggcttc taccaggatc agagtctctt cggagacata tttggcatag cgcagctgct
     2101 ctaagactct gcgctcctgc tcctgatcat agggtggatc catgaagatc acatccacgt
     2161 gcttttcata gatgcccgtc agtcctgcgc aggcatcctg ttttaatact atagcacggt
     2221 ctttcatttt cgtaaatgcc agattgtcct cgatacatgc cagggctttc ggcgcatttt
     2281 caataaaata ggcttttctg gcaccgcggc ttaatgcttc gatgccgatg ccgccgctgc
     2341 cactgaaaat atccacgaac acacagtccg gaatataggt ctgcagcata ttaaaaaggg
     2401 tttccttgat ccggtcggtg gtgggtctgg tatccattcc ttccggagtc ttaaggggca
     2461 gacttcttgc agttcccgca atgactctca tatgattact ctccgttttc tctataaatg
     2521 ttgtatgaat gattctgttt aaaaagttac actctcactt tggtgccctt gctgtcacgt
     2581 ttgccgagtt tcagattatt caggatgagc tccttgccct tatattcaat ggtggtctct
     2641 acggcattct tcgtataata tacattttcc acctgatcac cgtcgcccag tttcataccg
     2701 cggacgccga tggcaccttt ctttttctcg gggatctcat cgatggagaa gcgcaggaaa
     2761 aaaccggcct ttgtctgtaa tacgatattc cgctgatccg taagagccgt aacgctgaca
     2821 acgctgtcgc cctccgccaa cttcgtagct gcaacggtgc gcttggtcac atcgaactct
     2881 ccgccatcta ccaccttcaa catggactgt gcagtggcaa agatcacctg acacagattc
     2941 agttccttct gactggtgat atagacgatc tcttcggttt tctggctgaa gttactcacg
     3001 ttgtcaatgg gcgtgccttt gtcacggaac ttgccgtaag gcaggtccag caccttgata
     3061 gtgtgcatct gtccggtgtt ggtgaacagg cagatccttc cggtattttt acaggtgaag
     3121 acgtatttat tttctgcatc tgcggcttct ttattccgct cgtaagtaga gacatccaca
     3181 gtcttggcat agccgaatcg atccatgaga aataccacat ccatctcttc cagttctttt
     3241 tccttgtaga ccgcttcctg tccgttctcg atgacagttt tgcggggatg gctgtattcc
     3301 ttcttgaatc cgtccagttc attcatgatg acctgggcca tggaatctct gcggtccaga
     3361 atatcctcat agcgatagat gtttgccatg gtctcttcgt gttcgttgat cagtgcttcc
     3421 agttccagac cgatcaactt atacagacgc atctctaaga tggcattggc ctgtttctcg
     3481 gtgaacatca gctgggctgc catgatcctg gattccttgg acttgaactt gatgccatcg
     3541 accttaccct ctacgagaca ggcttttgcc atggcacgat ccttggatcc gcgtaaaatt
     3601 tcaatgacca gatcgatcac attgcaggcc ttgatcagac cttcctgaat ctccttgcgc
     3661 tcctgctctt tggccaatag attggtatat tttctggtag ccacttcaaa ctggaagtca
     3721 acattggcct tgatgatctg ctttaagccc atagtctctg gtctgccgtt tgcgatagcc
     3781 agcatattga caccgaaggt atcctccaga cgagtctttt tatagagcat atttacaaag
     3841 ttctcgggat cggcatcctt acgaagttcg atgacgatac ggatgccttc cttggaagat
     3901 tggttggaaa tatccacaat atcctgtgtc ttcttcgtct cggaaagagc ggccacgtcg
     3961 gataaaaact tcgcaatatt ggcaccgatc atggtatagg ggatctcggt gatcactaca
     4021 ttggtctttc cagattttcc cttctggagt tccactttgc cccgcagctt gatcttgccg
     4081 gttccggtct catagatgtc ttccagttca tccttattga taacgatgcc gccggtggga
     4141 aaatccggcc ccttcaggta acgcatcagt tcttttgttg tgatctcatt attgagcata
     4201 taagccttta cggcgtcaat gacttccccg agattatgag ggggaatgct ggtggccatg
     4261 cctacggcga taccttcgga accattaacc aacaggttcg gaatctttac cggcagtacg
     4321 gacggctctt tttccgtttc atcgaagttc ggaacaaagt ctaccacatc cttgtccaga
     4381 tctgccagga aagcttcctg ggtcactttc tgcagtcttg cctcggtata acgcatggct
     4441 gcggcaccat ctccttcaat gttgccgaag ttaccatggc cgtcgatcag gggcatgccc
     4501 tttttgaaat cctggctcat gactaccagt gcctcataga tggaactgtc gccgtggggg
     4561 tgatatttac ccatggtatc acctacgata cgggcacttt tacggtaggg gcggtcgtaa
     4621 cggatcccca gttcatgcat atcgtagaga gttcttctct gtaccggctt caggccgtct
     4681 ctgacatccg gcagggctct ggcaatgata acgctcatgg catagtcgat gaacgatttc
     4741 tgcatctcct cggagtattc tgtttttatg attctgctgt cgtccatttc taatctccgt
     4801 tcttctgata tctgatatta aatatcaagc gccgcatccg tagcgtgatc gtaaataaag
     4861 gcttttctgg gcggcacatc cgtacccatg agaagctcgg tcatctcgga agccatgcgg
     4921 gcatcctcga tctctactaa tttcaacaat ctgcgttccg gatccagtgt ggtctcccac
     4981 agctgttcgg catccatctc acccagacct ttgtatcgct gtaaggtaaa gggacccttg
     5041 tgggtcttgc ggtatttttc cagtgcctta tcatcataca gatatttttc ttctcccttg
     5101 gagggcatgg ccttatataa gggaggcatt gctatgtata cgtggccttc gaagatcaga
     5161 tccggcatga aacgatagaa taaggtcagc agcaacgtgg aaatatgtgc gccatccaca
     5221 tcggcatccg ccatgataat gatcttgtca tagcgcagct tactaatatc gaaatcgttg
     5281 ccgtagccct cggaaaatcc gcagccgaaa gcgttgatca tggtcttgat ttcggcgttg
     5341 gccagtactt tgtcaatgct ggctttctct acgttcagga tcttaccgcg gatcggaagg
     5401 atggcctgat acatacggtt tctggcagtc ttggcactgc cgcccgcaga gtcaccctct
     5461 acgatgaaga tctcacactt ggagggatct ctggactcgc agtttgcaag ttttccgttg
     5521 gaatcaaagg agaacttctg cttggtcagc atattggtct tggctttttc ctctgtctta
     5581 cggatcttgg ctgccttttc cgcacagccg ataatgctct tcaaagtctc cagattgcgg
     5641 tcgaagaacc ggacgatctc ttcgccggtc accttggcca cggccttggc ggcatcctgg
     5701 ttgtccagtt tcgtcttggt ctggccttcg aaacggggat ccgggtgctt gatggatacc
     5761 actgccgtca ttccgttccg gacatcggca ccggtaaagt ttgcatcctt ttcctttaaa
     5821 atgttcagct ctctggcata ggtattgatc acattggtga actgggtctt aaagccagta
     5881 aggtgggtac cgccttcaga gttgtaaatg ttattacaga agcccagtac attctcgtgg
     5941 aattcattca catactggaa ggccacttcc acagagattc cttcgctctc gccggaaaag
     6001 aaaatgacat catgtacggt ctccttggaa tggttcatat cctttataaa gccaatgata
     6061 ccttccggct catgataggt gaccttctcc ggctcttctt ttcttttatc ttcaaaaatg
     6121 atcgtaagct tcgggttcag gtaagcggtc tcatgaagac ggctcttaac atcatcttcc
     6181 ttgaaacggg tcttgtcgaa gatggtatca tccggcagga aattgatggt cgtaccggtc
     6241 tctctggtct tgcccactac gggaagcaga ccgtccacca gatccatcac ggggatacct
     6301 ctttcataac ggtcctcata gatgaagcca ccactcttga ctcttacagt caactttgtg
     6361 gaaagagcat ttaccacaga ggaacctacg ccgtgcagac caccgctggt cttgtaggcg
     6421 gaattatcga atttaccgcc ggcatgaaga gtcgtgaata ccaggcgctc cgcactgatt
     6481 cctttttcgt gcattcccac gggaatacca cgtccgttgt cttctaccgt ggcggagcca
     6541 tccgcttcca gagttacttt tatcgtatcg cagtatccgg ccagatgctc gtccacagag
     6601 ttatctacga tctcgtagat caggtggttc agacccttgg tggagacact gccgatatac
     6661 attccgggtc tctttcttac cgcttccaga ccttccagaa cggtaatact tgaggcgtca
     6721 taattattgt cttttgccat gaaatggaac tccttatcaa cacatgattg tctttagtat
     6781 agcatagatt ttgtgtttgg aaaagtaaaa aatacaaggg atgggggaaa cagataagat
     6841 aatgaaaact cccggaatac tactttccgg gagtaacaga atcattgaaa attacacatt
     6901 gattttttta cagcatgcca gtgcaaggga accggggccg atgtggcagg caacacttaa
     6961 ggacagtgga tcgatatgga tatcatagcc ggggaattcc gcctctacct cggtgcgaaa
     7021 ctgttctgct gcagctcggt cgtgggtata ggcaatctgc agccagatat gcttgtcctc
     7081 cgtcatgccg ccaaaacgct cgttgatgtc ctttttaata gcattgatca tcatggtttt
     7141 gccctgtgag gtggttcttg cctttgcaaa ggcatccagc ttttcaccct gaatggtcag
     7201 caccggtttc aggcgaagca ttgtaccgat ggctgcggcc gccggagtga tccgtccgcc
     7261 ttttttcaga taatacaagg tgtccagcat aatatagata ctgctgttga atttatcatt
     7321 ttccaggaat tctttgatct gggccgcgtc tttaccggct gccgcaagct gcagggcatc
     7381 cagtgcagac tgacgctggg ttacggagat ccgctggttg ttcacgacct gaactctgcc
     7441 atcataatct gccgcaaagg ccatggcact ctgacaggaa ccggacagac cgctgctcat
     7501 aggaatgtgt acgatctcgt catattcctg taacagtttg tcccacaggc tggtgacact
     7561 gtcaggagac ggctggctgg tggcaatatt ggcaccggat ttcagctttt catagaactg
     7621 ctcctgtgtc aggtcaatat cttcatagta tgtgacttca tcgatcataa aaggcatggg
     7681 cagaacatgg atttcgcgac gggctccttc tgcctgtgta attccgctgt tactgtcggt
     7741 tacaattgcg atttttgaca ttctggtatc taaaccttcc ctttgcataa attaacataa
     7801 attatctgat cctaagtata aaactttgaa cataaaaata attttactgt cagatggtcc
     7861 gaaagtcaac aacatttact gcggtatgtt ctttgaaata gttcaccaat gctcggctgt
     7921 cgtcatctgt cagctccgga tcggatacaa gcaactgttc cacggcatct gaagcctctt
     7981 ttaaaatatt tgcatctgta tatatatcac ccagtacaaa tgcaaattca ccgctctgcc
     8041 ggatgccgaa gatatctccc ggacctcgca gtttcagatc ctcggaagca atatggaaac
     8101 cgtcgttgga atggttcagg atctgtaatc gttccatggt ctctttggct tcggaagtgc
     8161 tgataaaaat gcaatagctt tggaattccc cacgtcccac acgcccacgc agctgatgca
     8221 gctgtgccag accgaaacgc tccgcattct ccaccatcat cactgtggca ttgggaacat
     8281 tgatgcctac ttcaattacc gtggtggaga ctaagacatc gatctcctta gcagcgaact
     8341 cttccataat acggttctta tctgccggac gcattttccc atgaagataa gccacctgta
     8401 cggaaggagg cagaactgcc cggagcttct ctgtataatc cactacattc tccaggtctt
     8461 ccgattcccc ttcttccacc ataggacaga tgacataagc ctgtctgccg gcagccacct
     8521 cttttgcgat aaattcataa gccttaggtc gataggaagt ccctacaaca cagtttttga
     8581 tcggcagacg attcgcagga agttcatcaa tgacagagac cttcagatcc ccatatagaa
     8641 taatggccaa tgtcctggga atcggcgtag cactcataac taacacatga ggctcactcc
     8701 ctttggcggc taatgtctcc ctctgcctta caccaaagcg gtgctgttca tccgttacta
     8761 ccagcgcaag ggaagaatat tccacttttt cctggatcag cgcatgggtt ccgatgatca
     8821 ggttagcctc tccggaagca atttttgcat aagcctcccg tttctccttc gccgtcatag
     8881 agcctgtcag aagcaccgga cggaaagcaa ttccatattt ttctatatag cctgaaatgg
     8941 tctcaaaatg ttggactgcc agtacttcag taggggccat cattgctccc tgataaccat
     9001 tagccgcaca catgagaaga gccagcactg ccaggatcgt cttaccggag cctacatctc
     9061 cctaaatctg gcggttcatg acaaaaggac atcccatatc ctccctgagc tccccccaca
     9121 ccttattctg ggcttttgtc a
//
LOCUS       DAWDHD010000096         9120 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_452_length_9120_cov_10.740320, whole genome shotgun sequence.
ACCESSION   DAWDHD010000096 DAWDHD010000000
VERSION     DAWDHD010000096.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 9120)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9120)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9120
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_452_length_9120_cov_10.740320"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2193 a         2428 c         1885 g         2614 t
ORIGIN      
        1 catcaactgc gtacagcgcc tggtcgatct cgtattcgcc aaaataatca tagacatttc
       61 caaaggtaga cagccagctt cccttttgtc cttcataggc attgtccagc agcagaactc
      121 cgttgccgtg taccttgtca taaccaaaat tgttgtaatc atagaagtta tccgccagat
      181 ctctcatggc tgtactccag tcggtactgc cgacttcctt cgcatgaaat gcctccgcaa
      241 gatcgggatt ctgcagatca tactctacgg attcggaaat cgtcacgatt acgatatccg
      301 cctggatctt ctcctcacac tctgctatgt actccagcag gttctgctcc tcttcatctg
      361 tcagcatatc cgcatagtca aatacacgct ctgccggatc ctgactgttg ccccggacag
      421 tcttgggtgt aagcagatga acgactgccg ctccgatggt aataaccgcc agcactccgg
      481 caatgatgaa ccatacctta aaatatttca ggtactgtct cagtgcttct ttcttaatat
      541 ctttttccat tgtcacgtta tccttccctc ataatttcta caccagactg gcgattgcat
      601 tcaccatgca tagaattgcc gccagtgtca caccaagtgt tcccgcataa gcccacacct
      661 ttgctttgga aatcggtgca gtacctacca gtttaccggt ctgtccgttt acatagaaag
      721 gatattcttt gcctttgtag ctatagtcat acttccatac tggcaggaga ccataactta
      781 cagaactgtc cgtcacgttg atgttttctc ccattttacg tacactggaa tatccagaat
      841 agctgtcatg cagcatcttc tgtgcatcct cctgcatctt ggctttggca cggctctcca
      901 ccagatcgga agtcatattg tagcgttcac tatgaaatcc agacagatat tctggcttaa
      961 actcctgcag ttccttgtaa tcaaagggtt ccatcagatc catgacatca tccggcattc
     1021 ccacggaagc atccacagga atcttcacaa aatcaatgtc catatcacgg acaatatcat
     1081 aatatgaggt ctcggtatac tgtgtattgc cggtcgtcca gctgcgcacc ttggtacctt
     1141 caccgtggaa cgtgcagttg gtattgtaat catagaacca gaacggtaca taatcaccgt
     1201 aaataccgtt cattcttact tccgacagaa aatccgtcgg tgcaaaaata catttctcaa
     1261 atttatcacg gatcagcttc ttgcagactt ccttacccat acggaaggga atcagcatat
     1321 gcggtgtgta ggcaccggaa atctgatcat ccacgatcac ataattgtca caataaggac
     1381 actgtgtagc ggaggtgttt accgtcatgg gaatctcgcc accacagttg ggacatacct
     1441 ttgtcacctt tttctgcgca gcaccctcgg ctgctgccat tctgctgacc ggctgtccgg
     1501 ctcccgctgc ctggaccgtt ccttccgcag tgccgttttc ctcaccgctt gtctcgatca
     1561 cgccgtcctc gcgttcttcg ctgtcctcac tctcacagaa aggacagaac atcttctttc
     1621 tctccgggct gtaaactaca ttaccgccgc agtttttaca tctaaagatc attttacccc
     1681 tcgccttctg ccttttacga catttttctt cgtagaacct tttttatctt accatagatt
     1741 ccatcgtatt tccatatcgt aaaatacaca taatcttttc ttatcgttcg tttaccatga
     1801 ttgtctcatg ctttcatcag gtctgcgtaa tcctccagca ggaatcggtg atagatcaca
     1861 tggcaccctt catcccgaaa acaataacag tagggatctc ccatttcctc cgggaaacgg
     1921 atcatgacat caaagtcctc gccccgcatc tcttccacat aaatattctc actgtaccgc
     1981 aaaaataact gtcggatatt ctccatctta cagtcatgct gcccgatcag gcggatcagt
     2041 tcatagataa atcccttctc tgcaaaatgc tcatggacat aagcactgcc ttcctccgga
     2101 atacagaggc agaaacggtc tcccttacgg ctcacagcca gttttccaag ctttattgtc
     2161 atgctctccg gctgtcctgc cagtgtaact tccatctgtt ccgccgtatc ctgtgtaccc
     2221 agcaagtgat tcagtgtaga taacggataa tacaggcgga tcttctctct gcggaagcct
     2281 aattttgcct gttcctccag aaccacatcc atgaggtttt tttctaattt ttcaaatgtc
     2341 attttctttt tcctataatt tcccataatc tgtcaaaaat aaaaactatg cagcggaggt
     2401 ttcctgtcct gcatagtttt tattatacaa tattcacgca tcagaataca attctattct
     2461 acggaaacag acattgccgt tccattctaa ttcagaaatt tgtccagttc cgtctctacg
     2521 atctgctttt tcttcacttt gggagcacgg atcacttttc cctgtgcgat gaccatatcc
     2581 acatttcgaa gcacccgtag atcttctaac ggattcttct ccaccacgat catatccgcg
     2641 catttgccgg gttccagtgt accggtgata tccccgatac ccgccatctc ggcgccgcgg
     2701 caggttgcgg tatacagtgc aaaagcgttg gatactccca catacttgtg gaaatagtac
     2761 agctcccgcc agaaatcgta ctgggtgatc caggggcatc ccacatcatt gcccagcacc
     2821 accggaatat cattggcaat ggccgccttc gcacagtcga taataccttc aaatacaaca
     2881 ttgccattaa actgctccac ttcagaggca ttggtaatgg atcggtcaaa caatgcataa
     2941 ggaagtgctg gagacaatgt ggtacacaaa aatgcattat gttccttgaa aagtgaaatc
     3001 atttcttcgt ctgcttttgc accatgttca atggaatcca ccccgttttt cagagctgcc
     3061 tttacaccct ccggagattc cacgtgggct gccaccatat accccatggt gtgggccttg
     3121 tcacagactg cctttaccat ttcaggggcc atcttcagtt cgccgggcac acccttttcc
     3181 ttcgcatcca gcacgccgcc ggtgatcata agtttcacca gatccacctt ttgtgccttc
     3241 gaagtctcta agtgctgcag cgcttcttcc acgctgtctg cggcgatagc tacagagcct
     3301 gccatatgtc cgccgggcac agagattcct tcattggcag ccagaattct ggggccgggc
     3361 tttttaccag cggcaatgtc gtcccgcagt ctggtgtcaa aatctcccag tccacccaca
     3421 gtacggatcg tagtcacacc gctgtacagt tcgtcttttg caaaaccgca gaccatattg
     3481 taggcgatag cctttgtcag gccattgctc atgatctttt tgaccagtgc ttcattgtca
     3541 cgctgtttct tctggggctt tccgttgcct gccagatgga catgcatgtt gatcagcccc
     3601 ggcaggatat attttccctg taagtcgatt tcttcatatc cggatgctgt cagattccgt
     3661 ttcccggttt cttccgctgg cagaagctct gtaatcctct cattttccac cagaataacc
     3721 tgcccctcct gtacctgcat gtcttttgta ccgtttaaga tttttccatt ggtaaataca
     3781 tatttcatat agtgtttcct gactttccat gtgtctcaga ccttcaaaac ctaaaacaaa
     3841 aatcatagcc ttattccata gccaaaagcg agtcatgcta taatataaaa ttatacctta
     3901 taaacacccg accaaccaca ttcattttaa tctatctgtc ataatctacg taagaaaatg
     3961 acataaccca tcattcctga tgatagttta gtgatagaaa aaggtgatac ttattaaatt
     4021 atataacata acaaaagagg attccattgt tggctttcaa caaagaatcc tctttttgca
     4081 agggttttaa gcccttattt tttgtgcaat tgttctatta cttatagttt acgatctgtc
     4141 cgaagtcagc cttcagggaa gcgccgccta ccagaccacc gtcgatgtct gcctgtgcaa
     4201 acagctcggg agcgctggaa gcagatacgg atccgccgta ctggatacgg atagctgctg
     4261 cggttgcatc atcatagatc tcagcgatgc actcacggat tgccttacat acttcctgtg
     4321 cctgctcggt agtagctacc ttgccagtac cgatagccca gataggctcg tatgcgatga
     4381 cagcagttgc tgcctgtgca gcagttacgt tcaggaatgc gatcttgatc tgctgacgga
     4441 tgaagtcgat ggtaacaccc tgctctctct gcgtcagagt ctcaccacag cagatgatgg
     4501 gagtaatgcc atgctcgaaa gccttgagaa ccttcttatt cacggtctcg tcggtctctg
     4561 caaagtactc tcttctctcg gagtgtccga tgatcacata cttcacgcct gcatcatcca
     4621 gcattgcggg agaaatctcg ccggtgaaag cacccttctc ctcgaaatac atattctccg
     4681 caccaatcat gatattggaa ccctttgctg cctccatagc gggaatgatg tcgattgcgg
     4741 gaacgcacag aagtacgtct acttcatcat ttgctacgag gggctttaat gtattgatca
     4801 gctctactgc ctggctggga gtcatattca tcttccagtt gccggcaatg atcttctttc
     4861 ttgccataat catacacctc ttcttttctt atattgtgtt tcccacagga tgcatctgtg
     4921 cctgcaggca ggttcgaata cattccgtgg gaaagcttta ttatttctat ttatttgtcg
     4981 tctgctgctg ccacgccggg aagctctttg ccttccagga actccaggga agcaccgcca
     5041 ccggtagaaa tgtggctcat cttgtcagcg aaaccaagct ggttaacagc tgctgcagag
     5101 tcaccaccac cgataatggt agttgcatcg gtctcagcca gagccttagc tacagcgatg
     5161 gtacctgctg ccagggtagg attctcgaat acacccatag gtccgttcca tacaacggtc
     5221 ttagcggtct tgacagcctc agcatacaga gctgcggtct taggtccgat atcgcatccc
     5281 tctctgtctg cgggcatgtt ggtaacatca tatacctcaa cctcaacagg agcatcgatc
     5341 ggattaggga aatcagcgat ggttacagag tcaacgggaa gtaacaactt cttgcccagc
     5401 ttctcagcct tttccatcat ttccttacag tagtcgatct tggtatcatc taccagagac
     5461 ttaccgatct catagccctg agcctttaag aaggtgtaag ccataccacc accgatgatc
     5521 agggtatcgc acttctctaa caggttggag ataacattca gcttgtctgc aaccttagcg
     5581 ccgcccagaa tagctacgaa aggtcttacg ggatcttcta cagccttacc taagaagttg
     5641 atctccttct ccatcaggta acctactacg ttctgaccac ccttagcggt gatgaacttg
     5701 gtaacacctg ctacggaagc atgtgctctg tgagaagagc caaatgcatc acatacatac
     5761 agatccgcca ggtcagccag ctctttgctg aattcctcac cgttcttggt ctcttctgcg
     5821 ccgcggaaac gggtgttctg aagcagaacc acatcgcctt ccttcatgtt ctcaacagcc
     5881 ttggtagctg cttcgccggt tacgttgtag tctgctacaa aagtaacctc tttgcccagc
     5941 ttctcggaca gtctcttggc aacaggtgcc agagattcgc cttcgttggg gccattcttt
     6001 accttaccca gatgagagca aagaattacc ttgccgccat cgttgatcag cttcttgatg
     6061 gtaggcagcg ctgcaacgat acgggtatcg tcagtgattt caccgttctt taagggaacg
     6121 ttaaagtcgc atcttaccag gactctcttt ccctttgcgt taatgtcatc tacagatttc
     6181 ttatttaatc ccatggtatt aacctccaat tattttagta tctaaaaagg gcccggtcct
     6241 tgtaagaccg gaccctgata ggtctttacg cgttaacgcc tgtcggctta tgctaactcg
     6301 ctgaagtact tgatggtacg aaccatctga gaggtgtagc tgttctcgtt atcataccag
     6361 gatacaacct gtacctgagt attgccatct tccataggag caaccatggt ctgagttgca
     6421 tcgaagatgg aaccgtaggt gctgcccacg atatcggaag atacgatctc atcggtgttg
     6481 taaccgaagg actcggtaga agcagccttc attgctgcgt tgatctcttc cttggttaca
     6541 gcgcccttaa ctactgctac cagaatggtg gtagaaccgg taggggtcgg aacacgctga
     6601 gcggagccga tcagcttgcc gttcagttcg ggaattacca ggccgattgc cttagctgca
     6661 ccggtagagt tgggaacgat gttgcaagcg cctgcacggc ttcttctcag atcgcccttt
     6721 ctctgaggtc cgtcaaggat catctggtca ccggtataag cgtgaacagt ggtcatgata
     6781 ccgctcatga taggtctgca atcgttcaga gccttagcca taggtgctaa gcagttggtg
     6841 gtgcaggaag ctgcggagat aacggtatct tcgggcttca gggtcttgtg gttaacattg
     6901 tatacgatgg taggaaggtc gttacctgcg ggagcagaaa taacaacttt acgagcaccg
     6961 gcagtgatat gtgcggaagc cttctcctta gaggtgtaga atccggtaca ctccagaact
     7021 acatcaacct ttaattcacc ccaaggaagc ttgcttgcgt cagcctcttt gtagatggtg
     7081 atggtcttgc cgttaacggt gatgctgtcc tcgccagcct caacggtgct tgcatacttg
     7141 taaacaccct gagaagaatc atacttcaac agatgtgcca gcatcttagg gctggttaaa
     7201 tcgttgattg ctactacttc atatccttct gcatcgaaca tctgtctgaa tgcaagacga
     7261 ccaatacggc caaaaccatt aattgctact cttactgcca ttttagtttc ctcctaaaat
     7321 tttaatttta tatcttttgg ctttccgtta ttcacagatt gtcaaaattt tatcagcaaa
     7381 gacattttac ctaattcctg attataattc aagatgtaaa tgaaaaatat ttgtgatttt
     7441 gtggtaaatt ttacagtttg tttagaaatg ctatggtttt tcccttcatt tttgctataa
     7501 taggttcgta ttatgataac cagaaaggat gaaaaatatg tctatcaaag cagattgtca
     7561 tcttcactct tctttttcgg gagattccca ggctcccatg ccggagatga tcgataaagg
     7621 tctcgctctg ggactggaga ccatgtgttt taccgagcat aacgattttg attatccccg
     7681 caccgaggaa ggaccgggaa caatcttttt actgaatacg gattcctatc tgtacgacgt
     7741 gctccgctgc cgtgcgaaat atgaagggaa aatgcggatc ctcttcggcg tggagctggg
     7801 attgcagcct catctggccg accgcaacga agattttatt tccgcctatg attttgattt
     7861 cgtcatcggc tccactcatc tctgcaaagg aatggatccc tattatccgg aatatttcga
     7921 gaaattctcc tccgaggaag aagccatccg ggcttatttt gcagagacac tggagaatct
     7981 gaaatgccat gacgattatg atgtattggg acatctggac tatatcgtcc gtgtggctcc
     8041 caccaaggac aagaactatt cctatgacaa atataaggat gccattgatc cgatcctgga
     8101 gcttttagtg cagcagggaa aaggtctgga actgaacacc ggaagcttcc gctacggtct
     8161 cagacagcct catccctgta ccgatgttct gcgccgctat catgagctgg gcggtgagat
     8221 catcaccgta ggaaccgatg cccacaaacc tcaggatgtg ggatcctatc tggatcttgc
     8281 ctccgagatc ctgcagctgt gcggtttcaa atattacaca atctacgaaa agcgcagccc
     8341 ggaatatcac aagctgtaat tttctttatt ccctatcctc caagctgaat gtgttccttc
     8401 atcagctcgc gaagcaacgc atccgctgtc catgagacat tatacggtgt cacaatcgcc
     8461 ttcagggcaa ccggtggtaa gccggttgcc ttatattctg ccaggaatgc atccacctgt
     8521 tgcgaatcca ttccggaaaa caccagcatt tccattgcaa aatcaggacc gttatatatt
     8581 tttccattct tatgaaaacc gctgatttcc gccagtgttc cgatcttctg tccatagtcc
     8641 ctgcggttca ccgccgtcac atccacacct attttctccg ctgccacacg cacagcccgg
     8701 tctttttgtg tactcacgcc aaacaataat atttttgcca ttatatttcc tcacagtcca
     8761 tcacccggaa gccgttcttt tccagcaatt gtgcaaatac tccctgtccg gggatgagtt
     8821 taccggtaaa gtttccatca taaatctgct ttacgccgca gctggggctt cgtgactgta
     8881 agattgccag atcgatgcca ttctctttgg cgatccgcag agcctcctct gcgccttccc
     8941 gaaactgcct gtccacattt acgcctttac agtttatgac ctcaccgctc acgatctccg
     9001 acggattcct cggggtcgga agtcctccca gcacctcagg acataccggg atcacgtcct
     9061 gcccttgcag atatattgcc tcacgctcgc ggtaattatt tttcaggtta tttttagagt
//
LOCUS       DAWDHD010000097         9096 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_453_length_9096_cov_13.129079, whole genome shotgun sequence.
ACCESSION   DAWDHD010000097 DAWDHD010000000
VERSION     DAWDHD010000097.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 9096)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9096)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9096
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_453_length_9096_cov_13.129079"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2112 a         2561 c         1845 g         2578 t
ORIGIN      
        1 ccaacagatc cgaacagttc catcttctcc ttaacagtag ccttgatagc ttctgcgccg
       61 ggagccagaa gcttacgagg gtcaaatccc ttgccttcca gatccttacc agcctcgata
      121 tacttgcggg tagcatcagc aaatgccagc tggcactcgg tgttaacgtt gatcttggat
      181 actccgaggt caattgcttt cttgatctga tctgcaggga ttccggtacc accgtgcaga
      241 accagaggaa gctcaccggt cagctcctgt acagctgcca gagtctcgaa gcttaagccc
      301 ttccagttag cggggtattt accatgaatg ttaccgatac ctgctgccag gaagtcaaca
      361 cccagatcag cgatagactt acactcctta ggatcagcgc actcgcccat accgataacg
      421 ccgtcttcct caccaccgat ggaaccaact tctgcctcga tagaaagtcc cttgctgtga
      481 gcagccttaa ccagctcagt ggtcttctct acattctcag cgataggata gtgagaacca
      541 tcaaacatga tggaagagaa accagcttcg atgcacttgt agcagccttc aaaagagcca
      601 tgatccagat gcagcgcaac gggaacatca atgtccagat cctttaacag tccgttgacc
      661 atgccaacga ctacatcata accgcccatg tacttgccgg caccctcgga tacacccaga
      721 ataacagggg aacgaagttc tttagcggtg agaaggatgg actttgtcca ctccaggttg
      781 ttgatgttga actgacctac tgcatagtgg cctgctttcg cttttttgag catttctgtt
      841 gcggatacta acattttaat acctccattt tatatactca ttcttgttac gatatacgaa
      901 atgttataca ttcgtaaaag cacgaaaaac acatttcgaa taatcacttt gtgactatcc
      961 aaactataac acattctttt caaaaatgga acagcttatt gttaaatttt taccaaattt
     1021 taagattcct tctgttccac ccggatgtct acgccccggc agatgttctc aatcactact
     1081 tttgtatggg ttccgccata ttctccatcc agagtccagg aaagaggctc tgcggaagtg
     1141 atctccacct ttgacgtccg gaagcagtac atagcatcgg tatcgatatc ccggttcagc
     1201 agggatacca taatgttatt cagttcaatc gggttcttcg gacgtttgat cagcgttact
     1261 tcgaaaacgc cgtcatccag cctgacgttt ttgccggtga tccgcttaaa tcctcctacc
     1321 gatgcggaat tggtcaccat tccgaagata aaatcatcca cgatcgtagt atcgtcataa
     1381 gtaatccgca tgggatagga acggatcgcg gacagccgct tcattccttc cagcagatat
     1441 gccatatgcc ccagcacatt tttcatatcc tgtccggtct cataagagac atccgtgaat
     1501 agtccgaaag ctgcgatata gacgaacaca tcctcattaa aggatcccac atcacaggcg
     1561 aaatcattcc catttacaat attttccgca gctctcacca tattcttcgg cagcccgagg
     1621 cttcctccga aatcattggt gctcccggcc gggatatagc caataggcgt acggaagcca
     1681 ctgcggatca tgccggtgac cacctcatcc agcgttccgt ctccgccgct gcagaccacg
     1741 atatcatatt ctttgctgcg cttctcggtc ttctccatgg catcctcact tctctgcgtg
     1801 ggatatacgg tgatctcata accgcccttg gtgaatacat ccagaatatc cgccagtttg
     1861 ttgcggattt gtgcctttcc agctttggga ttatagataa ataacatctt cttcataata
     1921 ctgccctcaa gatacaacaa acgggactaa cagaattctc catcagcccc gcttcttctc
     1981 atgttatgtt cttaagccac ctgaccgctc aaaaactttt caatgttgtc cacagcggtc
     2041 tgctcatctg caccctctgc agttactgta agctcttcgc ctgcacaaag tcccaggctc
     2101 atcataccca taatactctt ggcattgatc cgcttatcgt ctacttcaaa ataaatgttg
     2161 ctttcgtact tgctggcttc ctgtactaac agtgccaccg gtcttgcgtc caggccatct
     2221 tccagtctga ccgtaatact cctctttgtc ataattttcc tcctctttta cgtccggatt
     2281 ctatctgcca gttcgctgag tttacgtaat ctatggttca caccggattt tcccaccggc
     2341 ggatccagat actctcccag ttccttcagc ggagcgtccg gattctccag cctgacctct
     2401 gccatctggc ttaagggctc gggcagattc tccagtccgt agtgatcccg gatataaata
     2461 atatcctcta tctgtctggt cgccgcagtt accgttttgg taatattggc ggtctcacag
     2521 ttcactctcc gattcacaga attgcgcatt tctttcacaa tcctcaggtt ttccagcttc
     2581 atcagggcaa tgggtgcttc gcacacattg agaagatcca caataccgga tccttccttc
     2641 agatagacca cgtaatactt cttccgaagc acaatcttgg cttctatgtc aaatccctgt
     2701 atcacactct gtagttgtct cgctttatcc tcatcggtgc atacaaattc caggtgataa
     2761 cttttctgcg gatcgctcat agaaccaatg cagagaaaag ctcctcttaa aaatgctctt
     2821 tgacagcagg aatttttaat cagcaggggg ctcacaggct cagacagatc cccgacctta
     2881 tcgatcacac tccgcatctg ttcctcgctg attttaacat cagtatctat attatatgtt
     2941 tttttcaata atgtaaagcc ttttctgaca accgcctcgt tttccgtctg gaaaccgatc
     3001 atgaaatctc cttctgcgga gcgtccgtat tgtccactga agttcatgag tgcggctagc
     3061 tcagcgatct ggcaatgtct tgccgggctg atgtgttttg ccagttcttc ctttacttca
     3121 ctcgaaaatg acatgtcaga ttccttctct gggtacatca cggtggtaca gcttgatacc
     3181 ataatgacct tccgccttca tacgttcgta caatttattt gctaaagtaa cgcttcggtg
     3241 cttaccgccg gtgcagccga ttgcaataac cagacggtat ttgccttctt taatataatt
     3301 cgggatcaga aactgaatca tgtcggtcag cttgtccagg aatattccgg tctccggaaa
     3361 actcatcaca taatcctgca ctcctttatc attaccggtc tggagcttca gttcctcgat
     3421 ataataaggg tttggcagga atcttacatc aaagaccaga tcggcgtctg cggggattcc
     3481 atgcttgaac ccgaaggaca tgaccgttac cataagacta ttgtattctt cgttctgtac
     3541 aaaaatacga tccagctctt ctttcagttc tctgactaac agattggagg tatcgatgac
     3601 gtaatctgaa tttttgcgga ttgtctccag aatcttccgc tccttgtgaa tgccgtcctc
     3661 cactctgccg tctgctgcaa gagggtgcac acgtctggtc tccttatacc gttttatcag
     3721 agcagcatcg tcggagtcca taaacaaaat ctcgaatttg tagccttttt cctttaattg
     3781 ctccaaaatc cgggtcgcat ccatgaaatt ctggtctgcc cggacatcca gtcccagggc
     3841 caccttgctg atctcactgc ccggcatggc aatcagttcc acgaacttct ccaccagaga
     3901 taccggcaga ttatccacgc agtaaaatcc agcatcttcc aaaagcttta atgcggttct
     3961 ctttcctccg ccactcatgc ctgttactac tacaaatctc atatgcacct cgttattcta
     4021 tcgtaactat tcagaatccc attcgcaaaa tcacctcttc gaggcttcct tggagaacag
     4081 gtcttcctgt tctctgctca tgtggttact tcgccatata aatttgcgat tctgcgaaga
     4141 tgcaagcatc cgcagaaaac aaatttacat ggctctgaat agttacagga atttgatctc
     4201 gggttccagc tccacgtgaa aacgttcttt cactcttctc tgtacttccc agatcacatc
     4261 ttttacatct tctgcagtgg cattgccggt gttgataata aatccgcaat gcttttccga
     4321 tacctttgcg cctcctacgg agtatccccg cagaccagct tccatgatca gctgcccggc
     4381 aaaatgtccc tcgggacgtt tgaaagtact gccggcactg ggatattcca aaggttgttt
     4441 ttctctgcgg cgaattgcca gttcctccat tttggctgcg atctgctccg gatctccggg
     4501 ctgtagccgg aaagtcactt tcgtcacgac aaacgactgg ttctggatca cgctggtacg
     4561 atagccgtac tccatggtac tgttgtccag ttccatgatc tctccattac ggttgactac
     4621 cgtaacagcg gtcaccacct ggctcatctc tccgccgtag gctccggcat tcattacagc
     4681 tccgccaccg atggtaccgg ggattcccgc tgcgaactcc aggcctgtca ggccatgctc
     4741 tttcgctgct tttgccacct ggctcatcag ggcccctgcc tctgcttcca gatagcagtc
     4801 tctcacttcg atccggctca tatgcttacc aaccaccaga acaattcctg catatccctg
     4861 gtcgctgacc agcagattgc ttccgttgcc gatcacggta tagggaacat ccaccagccg
     4921 aaggtatttc tggatcttac agagctgctc ttcgttttcc agatagacca ggcaatctgc
     4981 cgggccaccg atccggaagg tggtatgcaa tttcatcggt tcctgtaaca gaactttatc
     5041 tgccggtaca atttctttta atgcttctat cactgctgca cttatcatgt tgtcaggctg
     5101 ctctttcttt ttgttataat tatgccgcga aagcctactt taataccagt gctgccgcac
     5161 cgtagattcc ggcatcattt cccagcttgg ccagggcgaa tttcgtctgc ttgcagggac
     5221 cgaaagcaca tctcttataa gaaggctcta tgtatttgaa taaaatgtct ccggccttgg
     5281 acacaccacc gccaatgaca aaaatctggg gatcaactac gctggctact gcggcaagtc
     5341 ccttgccaag atactcgccg aactgttctg cgatctcgat tgctacttta tctccggctt
     5401 tcaccgcatc aaatacggtc ttcgcagaga tttcaccacc tctgagcatg ctgggagcat
     5461 cgtctttggc cagacgtctg ttggccagac gggtaattcc ggtagcggat gcatactgct
     5521 ccagacagcc cttgttctta cagccgcaag tctctgtctc agcatcttca atatggatat
     5581 gtccgatttc accgccggca ccgttggtac cggtcaggat tctgccgtcg atgatgatac
     5641 ctccaccgac accggttccc agagtaacgg ctaccagatt ggaatagcct ttaccaccgc
     5701 cctgccacat ttcgcccagg gctgctacat tggcatcatt tccagctttc acagttacag
     5761 gcagatccag ttcttttttc agggtctctg ccagatcaaa ggcaccccag ccaatgttca
     5821 ctgcaccgtt gaccatatag ccgtcagcgt ccactgcacc gggagctcca attcctacgc
     5881 ctaccagttc ttctacagag atattcttct cctgcatttt gtttttgata gatgctgcaa
     5941 tatccggcaa taccttttcc ccgccgttat cttttactgt gggaatctcc cacttatcca
     6001 gcacctgtcc cttatcattg aaaagtccta acttgaccgt ggttcctccc acgtctaccc
     6061 caaatgcata tttactcatc gtcctcgtcc tcctcacgtc tttttgccag agagttctgt
     6121 acacgggtgt acagttcttt tgctgcgttg tagcccatct tcttctgacg gtggttgata
     6181 gctgccgtct cgcagataac tgccaggtta cgtccgggac gaatcggaat gttgtggcat
     6241 accaccttat tgcccagata ttcggtataa ttctcttcca gtcccagtct gtcgtactct
     6301 ttgtccttat cccagtcttc cagacggatg accagatcga tggtctgtgt atcttttacg
     6361 gacagtgcac cgaacagtgc cttgacatcc acgatgccga taccacgcaa ttcaataaag
     6421 tgttttgtga tatccggtgc agaacctacc agcgtatcat cactgacctt acggagttct
     6481 accacatcat cggttaccag acgatgacct cttcggatca gctccagtgc tgcctcggac
     6541 ttaccaatgc cgctctcacc ggtgatcagg acaccttcac cgtagacatc caccagtaca
     6601 ccgtgcacgg aaatgcaggg agccagcttg acatttaacc accggatgat ctcagacata
     6661 aagtcagaag tggatttttt cgtagataat aacggaatct gatgtcggat cgccgtctcc
     6721 aggaaaatag gatccggttt caggtcacgg ctgaacacga tacagggaat atcataagcc
     6781 atcagcttct cataagcttc cttacgagtc tcatccgtca tgccttccat ataagtgtat
     6841 tccacaaatc cgacaatctg cagtcgggtc gcatcaaaat gctcaaaata tcctgccagc
     6901 tgcagtgccg gacggttgat atccggctgt gtgatcttca catgtttcac atcaatctcc
     6961 ggtgtgagat tttccagttt cattttttcg ataacacggg ttaaacttac acttgccata
     7021 ctaatctcca tacctttccg cgaaaaaacc gccccggttc cccgcccttt tcattatctt
     7081 ttagtgtacc acaggttgtc ctgtctgtga agcaaaaaag gtcacgattt cctctgccgt
     7141 acgctcattc atttcgggaa tttccatcaa ttcttccacc gatgcctgcc ggatctcctc
     7201 cagtgactta aagtgcctca tcagggcctt tctccgggcc ggtcccaccc ccgggatatc
     7261 atccagcacg gacttcacct gagtcttgct gcgcagggat ctgtgatact cgatagcaaa
     7321 acggtgggcc tcatcctgaa ttctggtgat cagcttaaaa ccttcggaat gggtatcaat
     7381 gggaagctca atattatgat aatataagcc tctggtacga tggttgtcat ccttgaccat
     7441 gccgcagacc ggaatgtcga ttcccagttc ttccagtacg gacagcgcaa tattcacctg
     7501 tccgcgaccg ccgtccatca gaatcagatc cgggaacttc gtaaagcttc cgtactcctg
     7561 atccatctcc tgttcttcca gctctttgct ctcttccatg ccgtggcgga accgcctcgt
     7621 cagcacttcc cgcatgcagg cataatcatc cgggccggag acactcttga ttttgaattt
     7681 acggtaatcg ctgcgcttgg gcttcccctt ttcgtagact accatggagc ctacattttc
     7741 aaaaccgttg atattggaaa tatcgtaggc ttccatccgg gccgtgccgg tcagcggaag
     7801 ctgcaacaga tctgagattt ccttcaccgc accgatagta cggccttcct cacgcttcag
     7861 tttctccctg tcctgagaca ataccagttt tgcattctgg gctgccagtt ccaccagttt
     7921 ttccttactg ccgatctttg gcaccttcag gtagaccctg ctgcctttcc gttcagacag
     7981 ccatttttca ataagttctg cgtcctcaat ctcatattgc agcatcagct ctctggggat
     8041 aaaaggtgtt cccgcataga actgttttac aaaatcctgt aagatcgccg gtttgttatt
     8101 ttccggcaca tgtgtcatat agtaatgttc cctgccgatc agcttgccat cccggacgaa
     8161 aaacacctgt accaccgctt ccgtctcatc ctggtacagt gccaggatat ctttatcttc
     8221 accgacgccc tcggtgatct tctgcttctg ggatacctgt cgtacactgg agagcagatc
     8281 acggtacctt gcggcatcct caaattccat agcctccgcg gctttcttca tcttctcctc
     8341 cagatccttc aggatcggac tgtaattacc attcagaaat tccagtgcac ctgccacctg
     8401 ctgtctatac tcctccttgc tcacatagcc ctgacaggga gccaggcact gcttgatatg
     8461 ataattcagg caggggcgct ccagtccggt gtctctcggc agcacccggt tgcaggtacg
     8521 cagctggtac agcttgttca gtagctcaat ggtatctttg accgccgcag cactggtata
     8581 aggaccgaaa tagcgggact tatccttctt catggtacgg gaaaacagga ttctgggata
     8641 atcctccccc accgtcacct tgatataagg gtacgtcttg tcatccttca gcagagtgtt
     8701 gtatttcgga gaattctctt tgatcagatt gttctccagc accagcgctt ccagttccga
     8761 atccgtcaca atatattcaa atcgggcgat cagcgacacc atctggtcaa tggcaggacc
     8821 tctgccgata ttttcacgaa aataagacct tacacgattg tgcaggttga tggcttttcc
     8881 cacatacaaa atgacatcct tgtcatcccg catgatatac actcccggct tgcgggggag
     8941 cttttttaat tcttcttcaa aattaaacat aaatatttcc tcgcatgctt ctatcatacc
     9001 gattctgggg aaaaatacaa taagggattt aatattgtaa cttgagagtt agttaaatac
     9061 attggcaaaa tccacgaact cccggagcat gcgata
//
LOCUS       DAWDHD010000098         9046 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_457_length_9046_cov_12.310978, whole genome shotgun sequence.
ACCESSION   DAWDHD010000098 DAWDHD010000000
VERSION     DAWDHD010000098.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 9046)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9046)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9046
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_457_length_9046_cov_12.310978"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1992 a         2533 c         1983 g         2538 t
ORIGIN      
        1 tgcttcagcg atcatgccat ccacttcctc ctgcgtatac gtcttcacca ctacctgtga
       61 gctctcttcc gtctggcatc cggcttccgc tgctgccgcc agatcatttg cgttatcctg
      121 gaacttgctg tcgatccagg aatacaggat tccggcaaca taggtcacca gcaccagtac
      181 taacagacat ccggtcatga ccagaatgga tgctatggtg ttgtttttct tacgcttatt
      241 tgctctgctc agacgatcct cggggatccg tccgttttca ttttgttttt tcataagtac
      301 ccctgctctc ctttttcatg ctgtctctct ataactatgc agcattgcgg tctataagat
      361 tatatataac tattcagagc cgtgcaaatt atggcaatat gtacgtcgaa tgcttgcatt
      421 cgtaagaaca tttgacataa tttatacggc gaaataacca catgagcaaa aagcaagctc
      481 tgaaagagcg gttttgcgaa tgcgggctga atagttcctt catataatat aatcgaattt
      541 ccgacggaaa ggaagaggaa aaaatttgtt ttatgaatta ttaatgaatt ggcccttatc
      601 cggccatctc ccgcatttcc cgcaggatct ttttctccag gcggctgacc tgcacctgac
      661 tgattcccag gatcccggcg atctccgtct gggtcttatt ctgaaaatag cgcatacgga
      721 tcaactcccg gtctctctcc ggaagctccc caagcagctg tgccagcagc atctggtcca
      781 gcagtctgtc cttctccaca tcattttcca ccgcactgct gcttcccatg ctggctccca
      841 cacagccgtg ggccgcggcc actacccggt ccgccaggca cagttcgctg ccatcctctt
      901 gtccgctgct gctatagagg gattccacct ccgccccggc ttccatggcc aggatcacat
      961 cctctctgtc cagctgtgcc tcctcggaca gctcctgcag ggtaggctcc ctgcgcagtc
     1021 tgctctgcag ccgctgtctt gccagcctga ccttcagacc atcctccttg atggttctgg
     1081 acactttcag taacccgtca tcccgcagaa accgtttgat ctctccggtg atcatcggaa
     1141 cggcatacgt ggaaaaccgc acttccagct tcaggtcaaa tttatcaatg gctttcatca
     1201 gaccgatgac accgatctgg aacagatcct ccgggtcata acctctgccc agaaatcttt
     1261 tcacaatgtg atgcaccagt cccagatttt tctcgatcag tacttctctc gcttctttat
     1321 ctccgctttg tgactttccg atgagtactg atacttcttc catgcctcag tcctcttttc
     1381 cgatccagtc tccgcagcgc attttcttcc acatatgtac tctcgtgccc ttgccgggga
     1441 tgctctccac ttccagctca tccatgaagg cttccataaa ggcaaatccc atgccggacc
     1501 gttccagttc cggcttcgtg gtaaataacg gttccatggc tctggccaca tcctcgatgc
     1561 cttttccctg atccgatacc tcgatggaaa gcgtatcgtt ctcaagaatg cagtgcagtc
     1621 ttacctttcc cggatggata atgctgtacg ccgggcgcat ctccccatgc ttcgtataac
     1681 cggacagatt ctcgtatcca tggataatgg cattggttac tgcctccgat actgcggtct
     1741 tgatatccgc cagttcttcc agtgtgggat tcagatgggt aatgaaagcc gccaccgcca
     1801 ctctggcaaa gctttcatta caggagaccg cgtcaaatac gatctccatc tcattccggc
     1861 tgccctgcgt tctctgctgc aaaagttctt tttcttccgt cacctgaatg ttttccctct
     1921 ctgctaccat gttctcctca tttctgcgcc tattattgca ctgtttttca taattttttg
     1981 aataatttct ttcattttcg acagaatctt ccttccaacg tttaacccgg caggatctca
     2041 acgatcttgt gaagcccgga catacggaag atccgctgga tctgccggtc tgcatggatc
     2101 gcatagacat gtccgccgaa gcaggaaatc ttgcggtacc gccccatgat gatcccgatc
     2161 ccggaagaat ccatgaaccg ggtatcctca aaatcaaaca ctacattttc cacgttttcc
     2221 ctgaccaaga gcctgtccgc cccttcgcag atataggagg ctctgtggtg gtccacttcc
     2281 tccggcatgc gcaccatcag gcagttatca atgatctgaa agtcttccat atttcctcac
     2341 attccgctgt tttccagcat ttttactcaa aagaaaagag aacccgcgct ttactttacg
     2401 caagttctct ttcgtgtgtt gtgtgtcatt gtctgatggt atattgttct tccctactgt
     2461 gccgtattct ctgccagctg ggaacgatat ctctgggcac ttctggccgt ctccgtatcc
     2521 gggaacagct ctatgatctt gtcataggtg gtgatggcat tctgggcatc gtcactctta
     2581 cggtaagagt tgcccaggct cagccaggca tccttgttgg tctcatcgta aatgactgcc
     2641 ttggacaggt tctcgatggc cgtcgcgtaa tcctcgctgc ggtaagcatt gtagcctgtc
     2701 tcataatact gtgcggacag ctgcggcccg atcaggccaa gcaacgtctt gtacagcttc
     2761 tggaatgctt cggaagtact gtccacatcc acggatgccg aaatgctttc caggttagcc
     2821 gccgtctggg taatatcctg cgtctccaga tatgtggtgg ctgccgctaa caggctgtcg
     2881 atggtctgta gcgtaccgtc ggttcccaca tacccctgca gttcctgatt cagcgtatca
     2941 ttctctgtct gcagtgcctg catctgggtc tccagttcct ggatccggga ggtcttggtg
     3001 tctaactgat tgccgatgtc ggtgatggtc ttctgggcct cattgttggc attggatacc
     3061 gctgccggaa ccaccagaaa atacatcacg gccagaccaa tgatcagacc gatggcaata
     3121 ttgatcaggg aagagagacc tgcactcttc tgctccttca cattcaaagg ctgaatgatg
     3181 atctcattgt cactctggta gcgaaccgca tcatccttct tccggcgtcc ggtctgcttg
     3241 ctattctcgt ccggagtcag ctgtatctca acttccttca gataccgcag ggtcagcgta
     3301 ttgttgcggt cgatgtccat gcacttacgc agttctctct ccgctctgtc ccactgttca
     3361 ctgtccatat aaagcagggc taacagctga tgtgcccgga tgaacttcgg gttcagggac
     3421 agtacctttt tcagctggat gatagccaga tccttgctgt cctgattaca atagaccagt
     3481 gcctgattgt atttcttgat ggtctggtta atggcatcca ggcgggccgc attggactgc
     3541 agcatattga tgtaatcgtc cgcgatattt ttctcaggcc gcatattttt gctgatgacc
     3601 cattcgctca gtgccgcaac gacctctccg gtctcaaaat acaccagtcc cagcagattt
     3661 ctggcttcga tattgttctt attaaatttc aggctctgcc gtaagctgtt gatcgcacct
     3721 gtgagatccc gtactcctgc tttctccaga ccgtcattgt agaaacggtt ggacatacac
     3781 atgatcttct tgtagaggga tacatctgct ccgcaggacg tacagaaatc gtgctctgac
     3841 agatggcagc cgcagttata acaaaacatg ctctccctcc ttatttctct tctttttctt
     3901 cggattcttt tatcattttc accaacagat ccgccgcatc ggtcagatcc tgattgtaga
     3961 tagcttcctc agcgtcttcc acttccagac tgggatgaaa tttcttcagt tcttcttcaa
     4021 aattgatcat ggtccaaaga ctcctttatt tccccgacaa gatacaagct tcccgcgata
     4081 tagagccgtt ctcctggctg tctgcccgag acttccgtcc gcagtgccgt atccgcttcc
     4141 gtatacatag taaaccggtc ttccggataa gctgccagta aacctttcag ttcttccagc
     4201 gaaagcgacc tcgccgtccg catatgcgta aaagcgatcc ggtcgaataa tcccgacgtg
     4261 atcaccctct gtatcatatg cctgcaatcc ttatccgccg ccacaccaaa taacagtctc
     4321 ctgccctccg tgcagccgtc ctgttccacg gtatccagaa aagcccggat gccgtcatca
     4381 ttatgcgcac catccacgta cacctccgga agtacttctt ccatgcgtcc ctgccagaag
     4441 cagccaagga ttccctgtcg gatctcttcc acagtgggac acctggatgc ggcacacatt
     4501 ctgccgtcag gcatgtccgt actgcgaaac agcacttcca cggcccgcac tgccaatgcc
     4561 gcattttcca tctggtagcg tgcaatggta tgcaaagtaa gactaatata attataatac
     4621 tcagagcgca aggaaaaatc aatgtatttc ttccgaaatc caaggaaagt atagtccttt
     4681 ttcgacaccg gtatctgtat cgcaccaagt tctgacgctt tgcggcaaat taccgcactg
     4741 gcatccgcat ccccgtccag atacaccacc ggaacaccgg ggcgcaggat ccctgccttt
     4801 tccgcagcga tcttcgctgt ggtgtctccc agatactgta catgatccag actgatccgc
     4861 gtaatgacgg tcaggagctt attgtccaca taattcgtgg catccagtct tcccccaagt
     4921 ccggtctcaa tgatgcaata atccggcttt tcttctgcaa aacagagtaa tgccatgcag
     4981 aataggtatt cataaaagtt gagaacgaat ttctgtggcg cttcttcccc tcgctgcccg
     5041 tccccagtat tagcagaatt ggtctcctgc aaagcgtccc ataccgcaag aaacccctgt
     5101 aaaaaagctt cctctgagat catttcaccg ttcacggcaa aacgctcccg catatccacc
     5161 agatgaggtg aagtgaatac agccgtgcga tagcctgctt cccgtaggat cgaactcatg
     5221 taggcacaga cggagccttt tccattggtg cccgccacat gaatgatccg cagatccttc
     5281 tccggatttc cgagtttttt taaaaaccat ttaaggtccc tgctgtccgc aggatttttt
     5341 ttaacagtaa agcggggcat atccttcaga tatgccaccg cttcttcaaa cgttatcatg
     5401 tcattaccga atctcatcgt attccttatg ctttacgttc tttttattct gtacgtttcg
     5461 ttgcagatca tgccagctgt tctaagcgct ttgtcacctg ttccatcatc tgctcgtatt
     5521 ttgccagctt ttccttctcc tctgcgatct tcgcttcggg agccttggac aggaatctct
     5581 cattggacag cattcccctg gaacgtgaca gctcgccttc cagtctcttc tgctccttct
     5641 tcagtcgctc gatctcctgt gcgatgtcta ccagctctgc aaacggaatg tacagggttg
     5701 caccggggat cactacggat accgcatcct ctgcgattcc ggtcttgtca gcctggatca
     5761 tgatctcatt cgcataagca aggcttgcga agaacagctt accctcctca aaaatcctgc
     5821 ggatctcgtc attttcgctg actacataca cacttgcctt acggctgggt gctacgttca
     5881 tctcagtacg gatgttgcgg ataccgcgaa cggcttcctt gatggtttcg atggccttct
     5941 cctctgcggg gaatgcacga tcctcgctgt actccggcca cttgctgatc atgatggact
     6001 cttcttcatt ctgaatggtg cagaagatct cctcagtgat gaacggcatg taaggatgta
     6061 acagcttcag ggcgtcgatc aggacagtct tcagcgtcca cagtgccgca ttctggctga
     6121 ctgcatcgtc ggtgctgtac agacggggtt ttaccatctc gatgtaccag tcacagaact
     6181 catcccagat aaagtcatat accttctgta cggcgatacc cagctcgaag tgatccatgt
     6241 tgtcggtcac atcttttacc agagtgttta acttggacag gatccactta tctacgggct
     6301 gtaaatctgc tgccgcaggg gtggtgatat cttttccggt cttctccagt gcctgctcca
     6361 tgttcatcat gatgaaacgg gaagcattcc ataccttgtt ggcgaagtta cggctgttct
     6421 ctactctctc atagtagaaa cgcatgtcgt tgcccggtgc gttgccggtg atcagggtca
     6481 gacgcagtgc atcggcaccg tactggctga tgatctccag aggatcgata ccattgccca
     6541 gagatttgga cattttccgt ccctgactgt cacgtaccag tccgtggaac aatacggtct
     6601 tgaaaggctt ctctcccatc tgctcgtatc cagagaagat catacggata acccagaaga
     6661 agatgatatc ataaccggtt accaggacat ccgtaggata gaaatacttc agatcctcgg
     6721 tctgctccgg ccagcccagg gtctcaaaag gccacagtgc ggaggagaac caggtatcca
     6781 acgtatcggg atcctgtgta aagtgggtgc agccgcactt gggacatacc ttcggcatct
     6841 ccttggctac gactacttca ccgcagtcat cgcagtaata tgcgggaatc ctgtgtcccc
     6901 accacagctg acggctgata caccagtcgc ggatgttatc cgtccagttg aaatagttct
     6961 tctccatacg ctcggggatc agcttgatct caccattttt cactgcttct cttgcaggtt
     7021 tgatcaattc atccatctta acgaaccact ggtcttttac cataggctcg atggtggtct
     7081 tacaacggtc atgggtacct acgttgtgta catgatcttc gatctttact aacaggccca
     7141 tctgatccag ttcttccacg atggcttttc tcgcctcata gcggtccatg ccctcgaacc
     7201 tgccgccgtt tttattgatg gtggcatcat cgttcaggat attgatcaca ggcagatcat
     7261 gacgcttgcc cacctcaaag tcattggggt catgagcggg ggtgatctta acgacaccgg
     7321 taccgaactc tctgtctaca taagcatcct caacgatggg aatcacacgg tttacgatag
     7381 gaagcagtac ctttctgcca tgcagatggg tgtaacgctc atcatcggga tggatggcta
     7441 ctgcggtatc acctaacatg gtctcgggac gggtagtggc aaaggtcaga aactctgtgg
     7501 tgctgggcgt tccgtcctcg ttcatcagcg gatacttgat gtgccagaaa tgtcctgcct
     7561 gctcctgata ttctacctct gcatcggaga tggaggtatt acatacggga caccagttaa
     7621 cgatacgggc acccttgtag atgtagcctt tttcgtgcat cttcacgaac acttcggtca
     7681 cagccttgtt gcatccctca tccatggtga aacgctctct gtcccagtcg caggaagagc
     7741 ccagcttttt cagctgacca ataatacgtc cgccgtattc cttcttccac tcccaggctc
     7801 tctctaaaaa cttttcacga cccagatcgt gcttgtcgat gccttcttcc ttcagcttct
     7861 ggatgatctt gacctcagta gcgatacttg catggtcggt gccgggctgc cacagtgcat
     7921 tgtagccctg cattctctta taacggatca ggatatcctg catgctgttg tccaatgcat
     7981 gtcccatgtg gagctgtcct gtaatatttg ggggcgggat cacgatagtg aagggagtct
     8041 tactgtgatc tacctccgca tggaaatact ttttgtcaag ccacttctga tacagtctgt
     8101 cttctatgcc gctggggtca taggtcttgg ctaattcttt gctcattgct tcctccttaa
     8161 attctcctga aattaacaaa gccctctgcc gactctcgtc agcaaagggc gtcagtaacg
     8221 cggtaccacc tttgttcaca gcttgcgctg cctcgtgact tcttacaaag tcctatccgg
     8281 taacggggat aactcgtgag aacttacgcc cggaatatcc ggtctcggct ctccggcttg
     8341 gaagctacct tccacattcc tgtcttgaag cagccttcca gcgcatacat gcagccgctc
     8401 tctctggtaa ggggaaatgt gtactcctct tcgtcattgc cttttggatt tggttaatta
     8461 ctaataaaaa atatagtaca gaaattccga ctttgtcaag gaaatcttgt tttcctgcat
     8521 aattttcctt cagaatgtat ggttttcatg tataaactgg catggtaaaa aaaacatatt
     8581 ctggctcttt taagcaagcc actttttcta tacactgaat cctaacatat cccaccgaac
     8641 aaatgggaat tatgaaaaag aaattaaaag aggagatcat cattatggaa aagaaaaaat
     8701 catacggtgt attaattgcg ggagccattc tcctgatcct gggaatcgtc ctggcagtca
     8761 ttacccacaa taacagttat caggaatccg ttgcttatgg cagccagtta agcgatgtca
     8821 aggcacagct ggaagaagta gaagcttcca tcgaggcagt caccggcggc acaacagacg
     8881 gtgatctgga tgccctgaat gcgcagaagg atgaattgtc cgctcagaag tccactctgt
     8941 ccgaccagaa gctgtccgca gagcgccctt actcctacag cctggttctg gtattcttcg
     9001 ctatcctgtt aactgccaag ggattgtaca atgccatcgc aggcgt
//
LOCUS       DAWDHD010000099         9038 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_458_length_9038_cov_10.416899, whole genome shotgun sequence.
ACCESSION   DAWDHD010000099 DAWDHD010000000
VERSION     DAWDHD010000099.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 9038)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9038)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9038
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_458_length_9038_cov_10.416899"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2256 a         2523 c         1809 g         2450 t
ORIGIN      
        1 gggagacctt acatcgagga aggacattcc gtccgccatg ggcgttcgtg gatttatttc
       61 actaactctc aatttagtac tcttccgcaa tatcaatctc ttcctgctcg gcagctgtag
      121 tatctgcaga gtacggggtt ctgtggaggt atgccagcca gtagccgcat cctacgatcc
      181 cagcaccacc aacgatgttg ccaagggtaa taggcagcag ggcttttacg aggaaattga
      241 accaggtcag gctctctgct gcaatgccgt agacctgcgc tgtcagaaga ccgcatacac
      301 cgaaatacat atctgcgata ctgtgctcga agccgcataa tacaaatagc atcacgggcc
      361 agtaactggt cagaagcttg ccggagaccc gcttcgcagc aaaggacatc cataccgcga
      421 tgcacaccag aatattacac aggatacctc ttacgaaagc ctcacccaca cttaaattcg
      481 tccgggatgc cgcggcatta acaatggcct gtgccagctg ctctccgaaa agatccggtg
      541 tccgtcccac cactaccaat actgccacaa aagccgcacc gaggaagttg ccgatgtaaa
      601 caaacagcca gtttttcagc atcttccata ccgtgacttt cttctccagc actgccagta
      661 tgatcagatt gttgccggtg aatagctcgc tgccagctac cagtaccata gccatacccg
      721 ctgggaatac cgcagcgcct accagcttcg ccaccgatgc gatgccgatg gtgctggatg
      781 ccgtagtcga agcaattcct gcaaaaccga tgaacatacc ggcaaaaaat cccaacagaa
      841 tcgtcttaaa gatcgacaga tttaccttgt ggatcccgac ttctatataa tttcttgcaa
      901 tttccaaagg tgaatgcata accaaattcc tccttatcct tttttaacag ttccatcttg
      961 ccatatttcc aaaactcttg caagcaaatc cgaaaagaat atttccctct cttgattttc
     1021 gcacgttaat ttgctatagt gtttaaagga caaacggaca tcttccgact ttgttatact
     1081 atttatttgc agcaaataac agaatcgagg aaatctacat gagtactctt acatttggtt
     1141 ttatcggtct gggtctgatc ggcggttcca tcgcccgggc gatccgtcag aatctccctc
     1201 agtctcagat cattgcatac gatatcaatg cagataccct gcgggaggct gcccagtgtg
     1261 gtgttgccaa cgtgatcacc acaaggatcg atgcttcctt ctccgactgc gattatcttt
     1321 tcctctgtgc tccggtacag aaaaatgacg agaacctctc agctgtaaaa gaaattctct
     1381 caccaaagac actgctcact gatgtgggaa gtgtcaagag tgagattcat acagagatca
     1441 aaaaagccgg tctggaagga cagtttatcg gcggtcatcc catggccgga agtgaccgta
     1501 taggctttat caattccaag gccgttcttt tggagaatgc ctattacatt ctcacgccca
     1561 cggattccgt tccggaaaac aaagtcacgg attataaaaa tcttgtacag cagctgggtg
     1621 ccatccctct gattctggag ccggcacagc atgattacgt tactgcagct gtcagtcatc
     1681 tcccccatat cattgccgca tctctggtaa atctggtgcg ggacaaggat tctgcggacg
     1741 gactgatgaa gatgatcgct gccggaggtt tcaaggacat taccagaatt gcctcttctt
     1801 ccgctgcggt atggcagcag atctgcctaa ccaatacaga aaatatctcg acactgttgt
     1861 cctcctacat tgcttccctg caggggattc agcaggaatt aaatgccaaa agcggtgatg
     1921 acctgtatga actgttcgac agtgccagaa tctaccggga ttccttcatc aacactgcca
     1981 gcggcccgtt aaaatcttct tatgcaatca ccattgacat tgccgacgaa cccggtgaga
     2041 ttgccgccgt ggctacgatt cttgccctga aaaatatcag tatcaaaaac atcggcatcg
     2101 tccataaccg agagtacgaa ggcggcattc tgcgcattga attctatcat gaagaagcca
     2161 tcgagagatc cacaaagatt ctttgcgaaa agggctacag tctgcacaac aggaagtaga
     2221 aaccctgtac ctggctcatc gcactacaca aaaaagagat cacctacaaa gtgatctctt
     2281 tttccttatc tttccgcttc tgtttccttc gccgcactat tttctccaca cagccacata
     2341 cgagcagtcc aaacgcaccg ccacaggtat ccagcaccac atccgcaaaa cacccatagc
     2401 gtcctgggac aaataactga tggaactcgt ccgctccggc ggagacaaac acccagagta
     2461 cgatcagcag atacagtttc cggttcctct ccttccaggg tctccacaca ccgtacagca
     2521 gcactcccat gcatgcatac tcggagaaat gagccagttt acggattgga tgttcaaaat
     2581 aagttgccat actgtcgatc acattctgtg tccagtgttt cccggaaata gcattcagaa
     2641 gctccgcaca tttttccgag atcatagaac tcagtgagcc tgattcctca ccgtcctgac
     2701 cagaaaaagc gaaaatcaac agatataaca gtacaagtaa aatagcagcc acaacactga
     2761 ctgctatttt tttcttattg tttttcatcg tcattaatcg tctggtatac gcggattctt
     2821 gccgcatcac tctccgacat tccgatgaag acagccccgt acgtataaga atctcccttc
     2881 atttcgatcc tgacgatctg taagaacgca tgcagcactt catgcgtcca aatcctcagt
     2941 ttcgactcat acactgcacc gatacttaac acttccttgc acaaaaagcc cactccgttg
     3001 ggagatacat ctacgatatc tatgctgact tcctgcatgt ctccctggtc caggcgcttt
     3061 accatcaggg tggcactcat ttctgtacgc ttgcttcgtc ttctttcttc cattattttt
     3121 ctccttttaa gtcagcgata aacatggcgt ctccaaagct gaaaaatctg tatttttcac
     3181 ggattgcttc ttcgtaggcg tgcaggatat gttctcttcc cgcaagtgcc gataccaaca
     3241 ttaccagtgt ggactccggc agatggaaat tcgtgatcag tgcatccagc accttgaaac
     3301 aatatccggg ataaatgaat atctcggtat tgtcacagcc gggctgtacc atgccctgct
     3361 cgtccgctgc gctctccacg gtacggcaac tggtggtacc cacgcagatc acacgcccac
     3421 catttttctt tgtctcattg atggtgttcg ccgcttcttc cgtcacctga taatactcag
     3481 aatgcatatg atgctccagt acgttctctt cctttaccgg tcggaaagtt cccagtccca
     3541 catgcaatgt cacataagcg atcttaatgc ctttgtccgc aatctgctgc aacaattcct
     3601 gcgtaaaatg caatcccgcc gtaggtgctg ctgcggagcc ttcatatttg gcataaacgg
     3661 tctgataacg attcttatcc ttcagcttat gggttatgta aggaggcagg ggcatctctc
     3721 ccagcttgtc caacacttct tcaaaaatac cctcatattc aaaacgtatc agacgattac
     3781 cttcttccac agtctccagg acttctgcct ttaatagtcc atccccgaag gtcaggcgct
     3841 gaccgggctt gcatttcttg ccaggcttta ccagagtctc ccagacatcc gcttcttttc
     3901 gttttaatag aaggacttcc acatgtccgc cggtaccttc ccgttctccc aaaagtctcg
     3961 ctggaatcac cttggtgttg tttaacacca gacagtctcc gggatgcaga tagtcgataa
     4021 tatcccgaaa aatgtgatga gagatctctc ccgtattttt atccagtacc agcagtctgg
     4081 aggaagaacg atcctccagc gggtcctgtg cgatcagctc ctggggaaga tcaaagtaaa
     4141 aatcggattt tttcagttcg gtcgtctctg ccattacagg ctcgctcctt ccacaaatgc
     4201 tttatatccg cgatatgcct catatacatc ggccttgctg gtagcttccc acggtgcatg
     4261 catgttcagc actgccacac cactgtcgat gacattcatg ccatacagtg ccagaatata
     4321 tgcgatggtt ccaccgccgc ccaaatcgac cttaccaagc tctgcagtct ggaagttcac
     4381 ctctgctttg tcgaagatat ggcgcaaatg tgccagatac tccgcattgg catcgttaga
     4441 accggacttg cctctggctc cggtgaattt gttgatgacc ataccgccgc ctaagtatgc
     4501 cacgttcttt ttatcgaagc tggacgcata ggtaggatca aaagcactgc tgacatcgga
     4561 agacagcata cagctatgtg ccaggcatct tctcacgttc agttcgctgt attcgcctgc
     4621 cagattcatc acctctgcca cggcattttc aaagaaatga gactgcatgc cggtagcacc
     4681 tacggagccg atttcttcct tatctaccag aatacagcac atggtacggt cggtcacatc
     4741 gctgtccagc atcgcctgca tggaagtgta agcacagacc ctgtcatcct gaccgtaagc
     4801 cagtgtcatg ctgcggtcaa agcctgcttc tcttgctttt cctgcaggaa cgatctccag
     4861 ctctgcggaa acgaaatcct cttcatgaat atcgtaggtc tctttcagaa tatccagaac
     4921 acctgccttt actgcttctt ttccggcttc tttctcgtcc ttcttcttat ctttcttatc
     4981 gatcagcaga ggcttattgc ctacgatgat atccagagct tcaccctcga taacctttgc
     5041 tgcctttttc tccagctgct cctgtgccag atggatcaac agatcggata cgaagaatac
     5101 aggatcatcc tcgttctcac caatattgat ttctacacag gtgccgtcct ttttcacgat
     5161 gactccgtgg attgccagtg gaatggtcac ccactggtac ttcttcacgc caccgtaata
     5221 atgggtgtcc agataagcga atccaccatc ctcatacagg ggattctgct ttacatccag
     5281 tctggggctg tcgatatggg ctccgaggat gttcatgcca tctgtcatgg gcttacgtcc
     5341 gatctggaac atggcgatgg atttgttcat ccatacgctg tataccttat ctcctgtttt
     5401 aagagcagcg ccctctttga tcagactctc cagttcacgg taaccggact tctcaatagt
     5461 attgacaatg gtgtcaatgc actctctctc ggtcttaccg ttgtctaaga aatcacgata
     5521 ctgtgcgttt attttctcca gctcttttaa ctgtttggaa gaataggtct cccaggtgtt
     5581 tttcttttcc atatttatcc tcatttccgc atccgtcttc cggatactct tttactataa
     5641 tcataacagg agaggtccct cgccctctcc gtatcagtgt actacatttg cgtccgcagt
     5701 tcaattcctg tctgtcagat tttcatcctg ccccttagga tccctgtcga tctccgtata
     5761 atgttttttt ctgcgctgga tcttcttgat ctcccggatg cgtctacggt tctctcttgc
     5821 cagttcgctc ttactgctgg catgattcac cagtacccgg ttcaccggac ggaaataacg
     5881 gttcagataa gcaccgatca gaatgatata catgcagaaa tacatccaca acataatgat
     5941 cacaataatg gtcagacttc cgtaaatact atacccattt ccataggaca catacatgga
     6001 aaatccccag gaaaatatac tccatactac cgcagcaaag caggctcccg gaacctgttc
     6061 cgtgaatttc agctttgtgt ccgggatata tgtataaatc aggccaaaca aaagaatcag
     6121 caccgcccat acgaacagaa agcgaaaatt catcaggaga ctcacaaaca tctgcagctg
     6181 cggaatccta tgtaatgcaa tatccaccag ctgattacca aataccatga aaaacagtga
     6241 caaaatcaac actacaagca tgatcaacgt ataaaagctt gcaataaaac ggatcacgaa
     6301 gtaattacgc ttctcgtcca caccattgac cgcatttaat ccccgcatca gtgccatgac
     6361 acccttcgcc gccgaccaga ccatagcgat cagagccacc ggcagaattc ccacagaact
     6421 ctcatacaca tcaacgacca gactctccac catggcatct gcaatatccg gcgtcacatc
     6481 cgtcagcgcc gtgaccagat tctgctccgt caccggggta taaggaatca ccgcacagac
     6541 taccatcagc atcggaatca ccgacaaaaa caggaaaaag gctgtactcg atgcataagc
     6601 gctgatattc tgttttttca tctggctgga aaaatcacgc cagatgctga ttattgtcct
     6661 gagcatatct cgcatccttc atagaaaaag ttaaggatct gttccgcgcc aagcccctgt
     6721 agtgccatat ttttggcacc gttctgactc attcccacgc catggccgaa tccaccgccg
     6781 ataatgttat atcctaccac atttttctta tcatggctag gttcaatcac aaaaaatgca
     6841 ctgggaagga gattcggcat ggccacctca ctaccatcct gccttaccac cttcgtaacc
     6901 ccgtcacaaa gtactgctct gatattatat tccgagatca ccttaaaggt tcctttgtcc
     6961 gtgacaatga ccatttcgtc tgccacacca ccggggccac gttttacaac ggagatatcg
     7021 tttacttttg aaaaagcttt tatattttga ctgacatagt catcgtcctt cagagtcaat
     7081 attaactttc cattggcttc ataacgtttc ttcagcgtct ccactatccg atccacatca
     7141 atctctttta ccgtatatgt ccagcggtac cagccttcct gcgcttcata atcttccgca
     7201 tgtgtctccg tgataaaatc ccggaaagcc tcttcttccc tcagatcctc tggacctgca
     7261 tctccgttta cctcatttgt gtctgccgcg attgccccac cgtccgtctg catcaggctc
     7321 tccggattca gccggctgct cttcagataa tcaagtgcct ccgctgtctt ccatacattt
     7381 gccgtagttc ccacgccaca ggaggtagag taataataag tgttcgccac cgttccttca
     7441 tctgtcagga tcatctgtcc gtaggtctcc ttcaccgccg tggtagcact gtcagcttct
     7501 gtgatattat tgtagacctg ataggaagtg ctgtcgtcca catgggcgcc gtaccgggga
     7561 tagcccgccc gcagcatatg tccgtaggca taggttctgg cacagatagc ctgtgctttc
     7621 agggcttcca gcgggtagga tgccggcatt tcacttggca ctacactgta gagatattct
     7681 tccagaggaa gctcatttac cactgcaatt ccgtctgcgg tccgcagcag ctctatatgc
     7741 cctctgtaag aaggtgttcc ctgacttcga ttcacattga tcagccgtac ccggccggtc
     7801 agcactgtcg gtactatgcg gatccgttct cccacgaaat aatcgctgtc tgtgtcaatg
     7861 gtcacctcgt ctcccctgga aaacaattct tcctgcattt cctcaccggt tccgtactgt
     7921 agtagaaaat cactttccgc cgtcagtgtc accgtctggt gcaaaacatc cgaaaaatcc
     7981 gaagttttga tcaatacccg gattttgtcc atgttatctt ctctggccag caggataccg
     8041 cagatctcgt cattttcacg aataaagtcc gcatccatat agccgaatcg cagatcttca
     8101 gtagtacaca tggtaagctc ccggtacagg cgataccctt tgtaatcctc agccaacggg
     8161 atccggcccc tgccctcgat ctcgataccg ctttcatctg ccgacagcac ccggcctgtg
     8221 aacttctctg ttttcaggat aacggcactc acgaggtcat ccgtaagttc cacatctgcc
     8281 agctgttccc gcatctgatc cggtgtcggc agcctgcctg cctcattcgc ctctgtatcc
     8341 agatcatagg tctctcttac tccgtcacag aataaagtaa tctgcccagc ttccgcctcc
     8401 gtgatccaga cgtttttcaa ttcccgtact actggaatat gctgaggcag ggcctcttct
     8461 gtctccggtg cccccggctc ctcccgaccg cagctatgta acaaatttat cagaagtaaa
     8521 aaaagaagaa atgccgtacc taacacacac aggtatttct tccactgtat tctctgttct
     8581 ctggaaagtt tataataatt ctcaagcagt ctgtttttct tcaaatcatt tcccgcctat
     8641 tcagaatctg ttttatgact ttaaaagagc agtcccaaag gactgctctc tcttttgtat
     8701 tgttatcccc aaaatgccgc ctcttacctt ttgactccta gcaatgctag gtgggtaacc
     8761 gcaaccaacc ttttgccttc ccttccaatc ctccttgcgg agtgagggct tgacagccac
     8821 ttggcaatat aggaatcccg tttaaagtaa atgtaggttc aaaactggta agagttatcg
     8881 tacatctcag gttaagtata ttatagcgag ttaagatcca ttttacaact gtaattttta
     8941 ccaatattat tatctgcaaa aaaggctgtc tccgaagaga cagcctcaga ctgtagacaa
     9001 agctccttcc tgtgagggag cttttcttgt gtcatgac
//
LOCUS       DAWDHD010000100         8919 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_462_length_8919_cov_10.433100, whole genome shotgun sequence.
ACCESSION   DAWDHD010000100 DAWDHD010000000
VERSION     DAWDHD010000100.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 8919)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8919)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8919
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_462_length_8919_cov_10.433100"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2466 a         1920 c         2250 g         2283 t
ORIGIN      
        1 gatatcgagg tcgaggatga tgtgacggct tatatggaat ttgaaaacgg agccaccggt
       61 gtcttcgtga ctaccacggc agatgctcca ggaaccaacc ggctggagct gaccttcgag
      121 atgggaaaaa tcgtgtgcga ggaaggcaga ctattttttg ataaactgtc caccaatgtg
      181 agcgatttct gcaaaacgga gaaggaagga tttaaaaagc cggaatattc cagaattgag
      241 attgagacgg atggacaaaa cgagcagcat attggtgtat tgaaagcgtt cgcaggtcat
      301 attttaagag gaactccatt ggtggcagag ggaacggagg gaatccgggg gctgaccctg
      361 tccaatgcca tgcatctgtc cagctggctg gaccgcaccg tggaactgcc gttggatgaa
      421 gaattgtttt tacgagaatt gaacaaaaaa cgtgccactt ccagaaaaaa ggaagacact
      481 gatattgtat ttgatacgac aggcacttat taaggaggac aaaagaatga cagtaaaaat
      541 cacaggtttt gcagatgaaa tcgcaccgga actgacagct cagatcgcag gactgaaagg
      601 actgggaatg tcttatgtgg agatgcgtgg agtggatgga gaaaatctga tctatcacac
      661 ggatgaaaaa gtggagcaga tcaagaagac actggatgat aatggaatcc ggctttctgc
      721 cctgggatct cccttgggta agattctgat cacggatgat ttcactcccc attttgaaga
      781 gttcaagagg gcagtggaga tcagccataa gatggaatgc ccgacgatcc ggatgttcag
      841 cttctatctt ccgcaggaga gtgatccggc agcctatgag ggcgaagtct tcgacaggat
      901 cggtaaattc gtggactatg cctctgcgaa tgatacactg ctcctgcatg aaaatgaaaa
      961 agatatctat ggagcaatgg ctccggaatg caaaaaactc atggatacct tttacgggga
     1021 tcattttaaa gctatttttg actttgccaa ctttgtacag tgcggacagg atacgctgga
     1081 agcctatgag atgttaaagt cctatattgc ctatatccat gtaaaggatg cactgaagtc
     1141 agaccagagt gtagttccgg caggctatgg tgacggaaat gtggcggtga tcctgaagcg
     1201 tctgtcagcc tccggatttg atggattcct gtcactggaa ccgcacctgt ttgagtttga
     1261 gggtttccat gcactggaaa aggacggcag gagcattgct gtcaaagaaa agaaggtact
     1321 cagcggtctt gagacattta ccatagcgca tgatgctttg atgaagcttc tggacaactg
     1381 catgtaattc ccttacgatg agggagactg catcaggagg gcgcagaggc acccgctttc
     1441 tcaatatact ctgtgggagt atagccggta tattttttaa atacctggct gaaatactgt
     1501 ggattattgc cgaatcccac agacctggca atagttttga tattgtcatt atgatagtac
     1561 accataaggc ggatggcttg ttccatccgc tttttgttca ggtaatcgga aaaccggatg
     1621 ccttcctcct ttacaaattg tttgctcagg tagccgacac tgacaaagag atagttctct
     1681 gccagccatt ttaaagacag ggtctcacag tccaggtggg cttccacgta agattttaag
     1741 aggctgatgt tggcaccgcc cttagaggag gcagcctcgt ccttctcctg cagcagtacg
     1801 atctccgcca gcttccggat ttcggaggtc tgggtacagg aatacaggcg gcggaaaaag
     1861 tcacaggcaa tatcgagcgg cggctcttgt ccggagttat cgggattcag gaaaagtccc
     1921 agggcgatat tcttcgcaac atccggtgtc atggaaggag tgaagacgga atccagcaga
     1981 gatggaatat ccgtttcggc cggggaagcg gtaaatgcct cacgcagctt ggaaatccgc
     2041 cagggagcgg ccatgttgtt atggaagtcc ctggtttctc cggtttctgg gcggatcagc
     2101 aggatccgtt catagcggga tatcttggat atgatctgcg caaacaaatc ttccgaagtg
     2161 tcaccgtgga gaaaagaggt ttcaaaggtt acacactgtt gcggataatg cagctcctcc
     2221 aaagactggc ggatcttttc ctgatgggcc agggtgggag aggacaggac gaaaagagca
     2281 ctgtttttta cataaattac aggaaagaca ctttctgctc cgcaggaaag cagaatccgc
     2341 acgatatcct ttagaaaact tttcagatag gtttcttcta caaaggaaca gatacaggca
     2401 tatagtccct tttcgggaaa ggacagaagt ccctgatact ttttgaatac ctggggaaaa
     2461 ttatcaggct gatccaatgc ttccataagc agcccctgtt ctaatggcga ctgcatatcc
     2521 ttcagcagaa gtgcctgctc cgccttctgc tgtacctctt tttgcttttt ttcttcacgg
     2581 atcgtatcca gacagttgat cagctcctgc ttgtccgtgg gttttaagag atagtagcgg
     2641 accccgtatg tcatggcctg cttggcatat tcgaattcac cgtaaccgga taacaggata
     2701 aaagtgatcc gggagtcgat gcgggaggca cgttcgatca gttccagacc gttcaaaata
     2761 ggcatcttga tatccgtgat cacaacatcg ggatagtcat cacggatccg gtcataggct
     2821 tccattccgt ttttggcggt agcaatcacc tcgtagccga gggaggtata atcgatcatg
     2881 gaagaaagtg cttcacgtat gatctgttca tcatctacaa tcatcaatct gagcataaga
     2941 atcctctctt tctttgggaa tggacagcag tacaatggcc attccgtctt cattgtatat
     3001 agataagccg taccggtcgc cgaagagcag cttcaggcgg gagttgatat tcaccagtcc
     3061 cacaccggaa ccctggggag tgacctgcct gtgttccagc ttccagatga gatcatcctc
     3121 gaaatgggaa ccggtatttg ctacggtaat ctggtagacg gtgtcttttt cggaaatggt
     3181 gacggtgata tgacagacat cgtcactgaa ttccagagca tattttaatg cgttctctac
     3241 cagaggctgg atacaaagtg tcgggacata aatatgggaa atgtcatccg gcacatgcag
     3301 ctggaagtcc agccggtctc cgaaacggat ctgctggatc cggatataat cctttaaaac
     3361 gtccagttcc ttttttaaag ggatcaggtc ttctttgctg ttgacagcag cgcggaacag
     3421 attccccagg gaacggacca tggtggagat gtcatcggca ccgtattttt gcgccatcca
     3481 gttgatggta tccagggtat tgtagagaaa atgcgggttg atctgctgct gcagcatctt
     3541 gatattggta tcccggagca acagctgctt ttcatagttt ttatcccgca aagtctttac
     3601 attggatttc atttcgtcaa agctgacatg cagctgcccg atctcatcgg tgcggagact
     3661 gtaatcgtag tgagcggtgt cctccagatc cgggagtccc tgtccgaagc ggcggatctt
     3721 ctctaccaga agatccagat ggtgcagtat cctgcgcaga agcaacagtg ccagaaacag
     3781 agcgatcatc acgaccacgg cggagaacag gatcatccgg atcagaacgg tatggatcgc
     3841 agaaaagagc ggatcgtaat cctgaaaata cagataattc catccggtac gtggaaaatc
     3901 tccctggatg acaaaggtct ttcgtttatc tatggaaaga atagaataat ggctgctgcc
     3961 atgattcagc gactgcagca ggctggaata ctgttccgga tggtaagtct cctgtgggta
     4021 aaactgcgca tttcctgcaa acaggataaa acgggaatgc gtggagagag aatatccggc
     4081 attctccagg gcatcactga tcagctggtc caggtcgatc actatgtaca ggtaagccag
     4141 atgatccagg gtcagatggc gaagctgccg gatatcccgg taaagtacga tggtattccc
     4201 gggggaagcc ggtgcactcc agctggcacg cccctggcag gctgctgcat tttctgccag
     4261 ttcctccaga ggaaaagcat ccagatcgga agtatttccc atacagatca gtgtgtcgtc
     4321 aggaagtatc agactgatgg atttgatata gtcgtcatca aaaatataag gatagagagc
     4381 ctgatatatc gtctgctttc tctgggcatt acggatactg gtgggagcat ccatgatatc
     4441 ggtgagattc gtctgaatga tggtatcgga caggatccgg gcagaaccgg attccacggt
     4501 ctccattctt gcagagacga aggaagatac ataattcaga gactgtgcat tggaagtata
     4561 cagctcacgg gcataatact gggaaagaag ctgaatgccc acaacaaaag caaagaacac
     4621 aaaaagcaat gtcgttgaca ggatcagtag tattttctta tataaagaca gattattaaa
     4681 acgctgtgat aaattgctca tggaaaaacc tccgtatcat ggttattata tagtaaaaat
     4741 atgggaagtt caattttcat ccgtgggaag aaaacaaaaa gccgtttttt gaacactcag
     4801 aaaccttttt ttgaacacga aattatgcaa aatgccatat ggaatatgga aaaactgaaa
     4861 aaagtatgaa aatgtgacga aaaaaatgta agattatggt taaaaagcca tttttttgca
     4921 tcgacagaac cgttatttaa ctttaccatt atgcaaaatg gacataaaat aaattcatca
     4981 aaacaacaac gagggatgtg caggacacat acccgacaga ttgtatggga ggtataacaa
     5041 atgaagaaaa gaatgattgc aggtcttatg gctgtattga tgaccatgtc tctggctgca
     5101 tgtggaagca gcgcaggcag ttctgatgca tctggcacag gctccgctgc cacaggtgat
     5161 aacagcgcag cacaggctgc cagtggtgac aaagtaaccc tgagcctttg ctggtggggc
     5221 aaccagacaa gaaatgatgt aaccaagaag gcagttgacc tttacatgga acagaacccc
     5281 aatgtagaga tcaaggtaga gtttaccgac tggtccggct actgggataa gctttctgca
     5341 atggcagcag gcggcaacct tcccgatatt atccagatgg attactccta tctgcaacag
     5401 tatcagcaga gcggccagct tgctgatctg agccagttca ttgacagcgg tgtcattgac
     5461 accagcaaga ttgcagacag cgttatcgat agtggatcta ttgatggaac ctgttatgca
     5521 ttaagccttg gcagtaatgc tcctatgatg gtatatgaca aagaaatcgt agaacaggcc
     5581 ggtgtagagc ttcccgagca gctgaccatc gaagaactgt atgatatcgg acagaccatt
     5641 tacgagaaga ccggtgtgaa gacctactac gatggcggca tcaatatgat gcaggtcatc
     5701 gcaagaaccc agggatctca tttgttcgat gaactggcag caggaaccaa gactgcaagc
     5761 gaaacccact tcgctaatgt agataagttc aacaaggcag aatccgctat ttctcctgat
     5821 cttctggcag agaagaaccc ggatgtagta gagaccaagc ctatcattga cggaactacc
     5881 tggaatgact tttcttattc caaccagtat atcagtattg ccaacacagc cggtagagat
     5941 ctggaaatca ccatgtatcc tactaccagc gatgcaacca cccaaccgat gttcttaaag
     6001 cccagccagt tcttttctat cgccgagact tctcagaata aagaggaagc tgcaaaattc
     6061 ctggactggt tcaccaacag cgttgagtgc aacaatatcc tgctggcaga gagaggcatc
     6121 cctgtgaaca gtgatgtggc agaagcaatc aaacctaatg tggatgagaa ttcccagaag
     6181 gtatttgact atatcgcaga agtatccaag atcgcaactc ctattgatga tcccgatccc
     6241 tccggtaagg gtgaagtaga agcacaggcc aagaccattg ttgagaacct gcgttatgga
     6301 gatacagatg cagcgggagc agcagaagaa ttcgtaacca catctcagaa gattctgagc
     6361 gaggcggcaa agtaagacga gaagtaagac ttctgaagta agaccgttat aacaaattgt
     6421 aatcaaaggg cgcctggatc ccatccggca tcgggcggga gccgggcgct tgttatagaa
     6481 acggcagaag gagaggaaaa atgagcaaag aaaagacttc cccaaagaga aaaagaacat
     6541 tgggccagtg gctggatagt cctaacgtat ccggatatgt gtttgtcgga ccgttcatta
     6601 tcggatttct ggcattcatg atggttccca tgcttttatc ttttgcattt tcatttacaa
     6661 gatacaatat tttacagtcc ccgacattca ttgggctgga aaactacatt accatgttca
     6721 cggatgatcc gaaattctgg aaggtattcg gtgtcaccat gtattacgtg gtattttccg
     6781 tacctcttcg tctgatcatg gcattgatcg tagcaatgct tctggtgaag agcaccaagc
     6841 tgtccggctt ttacagagca gtgtattacc ttccttccat tatcggttcc agtgtggcgg
     6901 ttgccattct gtggaaaaga atgttcgcca gtgacggtgt gatcaacggt ctgttcggac
     6961 tggatatcgc atggctggga cgttccgata ctgcaatctg gaccctgatc attttggcag
     7021 tatggcagtt cggatcctcc atgctgatct tcctgtcagg attaaagcag attcccgtaa
     7081 gcttatatga ggcagccacc atggatggag ctggcggtgt ccataagttc ttcaagatca
     7141 cactccccat gctgaccccc acgattttct tcaatctgat caaccagttg atcaacggct
     7201 ttatggcatt tacacagagc tatattatta cacagggaaa accaagggat accacccttt
     7261 tctatactgt atatatgtac cagaacgcat ttacctataa caaacttggc tatggatgtg
     7321 cacttgcatg gtttatgatt ctggtagtag gaattttgac actgatcttg ttcaagaccc
     7381 agaacaagtg ggtatattat gaatcagaag gttgatggtg agcgtatgaa aaaaggatat
     7441 aaagaaaaaa gatttgctat cggagtgatt tatcatatct tcaccttctg ttttgcctgc
     7501 gtgatgatat atccgttagt atggatggta cttagttctt ttaaggatac caatgagatt
     7561 ataagaacgg catcggaatt gatcccgaga cacttctctc tggagaacta caaggtaggc
     7621 tggcagggat ttgccggata caatttcgga accttcttca aaaactcttt tgtgatcgca
     7681 ggtttatcca ccatcggagc agtggcttcc tctgctgtga tcggatacgg cttcgcaaga
     7741 attaacttca aattcaaggg attctggttc gcctgcatgc tgttgtccat gatgcttccg
     7801 ttccaggtag taatgatccc gcagtacata atttttaata aattaggctg ggtcggttct
     7861 taccttccta tcattgttcc ccagtggttc ggtcagggat tcttcatttt cctgaatatc
     7921 cagttcatca aggggattcc catggatctg gatgaggcgg ccagaatgga cggatgttct
     7981 acctatacga tcttcttccg tatcatcctt cctctgatca aaccgtcact ggtaaccagt
     8041 gcgatcttct cctttatgtg gagatgggat gatttcctgg cagcactgtt atacttaagc
     8101 gatcctctga aatatccggt gagttacgct ctgaagatgt tctcggatcc taccgcaggt
     8161 tctgactggg gtgctatgtt tgcaatggca accttgtccc tgattcctat atttgttatt
     8221 ttcctgacta tgcagaaata tctggtagaa ggaattgcat ccaccggtat caagggctaa
     8281 agctgttcgg cggggagact gttccggttc tccccgcttt tcttgttttc agataaaaat
     8341 cagaggagag tgtaatatgg acactgttag aaatcgaatc aaagctgttc tcgaagccgt
     8401 agagcagatg atatatagtg acagacaacc ggtaatttcc gtgaagatcg ccaaagcgga
     8461 ttaccatttt gaggaggaat tccacagaga aggaaaagat ttccgggatt tcggccgcaa
     8521 tgagacctgg ggagagctgg atcagcatta tttcttcctg gcagatgtgg cgatcccgga
     8581 gcagatggag gggcatacag tccgtgccgt gctgaatacc ggagccacag atatctggaa
     8641 tacggacaat ccccagatca tggcttatgt gaacggcaga tttgcagcca cgctggatat
     8701 gaaccatcag tttatcattc tgtcggagca tgccaaagca ggagagactt acgaactggc
     8761 tttttatgcg tattccagtg cgtatgcagg cacgtttacc ggtacgaatt tcttccgcct
     8821 ggagctggca gtctaccggg aagaggcggc gggactctat tatgacatgc aggctgtcta
     8881 tgaagctgcg gatcttctgc cggaggacaa tctatccag
//
LOCUS       DAWDHD010000101         8908 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_464_length_8908_cov_11.076584, whole genome shotgun sequence.
ACCESSION   DAWDHD010000101 DAWDHD010000000
VERSION     DAWDHD010000101.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 8908)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8908)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8908
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_464_length_8908_cov_11.076584"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2088 a         2531 c         1754 g         2535 t
ORIGIN      
        1 ccggtgcttt ctttcttttt tctattaact tttcctacaa aaactttacc ctacgtttta
       61 aaaattattt tctcaccact tacggtctct gtcccatagc gccttccagt gtcagggtct
      121 caccgctcat atacttgaag tcgggagaag ccagttgtac acagacacga ccaatctcct
      181 tctccacgtc accgtaatgt cccatgggcg gcatcttgac atttgccttg aaagcatcgg
      241 gatatgcctg ctcaaatttc tccaactgtg cggtccatgc cagaggacat acgacattga
      301 cattgatgcc atcgggtccc cactcagtag ctgcgactct ggtcagtccg cggatgcctt
      361 ccttggctgc cgcataggca cactgtccga agttaccgaa gagacctgca ccggaggcga
      421 agttcactac ggaaccctta gtctccttca ggtaaggata gcaggctttc atatagtaga
      481 aagcggcgta cagtccggaa tatacggcca ggtcaaactg ctcggtggta tgatctgcaa
      541 tagtcactcc ggaagcggaa gcctgcgcat tgttgatcag gacatcaatt ctgccaaaag
      601 tatcaatggc ctgcttcacc acattctgca cgatagcttc attgtcggca cctgcactga
      661 catccgcctg cacaggaagt actttgatcc catacttttt ctccagttct tccttcgcgt
      721 cggtcagctt ctgaacatta cgtccggtca gcaccagatt ggcaccttct ttggcatagg
      781 ccgttgcgat accataaccg atggaaccgc actctccatt acttaagacc gctcttcctc
      841 cgcctgtgat gattgctgtc tttcctgtta aaaatcccat aaataacctc cctttccaat
      901 ctgacgatct gctattctaa aatataccat gccttccgga acatatgtca ttgatttcta
      961 tttatgatac aagtccatgt aagcacttac atttattata gcacacttgt cagacttttc
     1021 cacaactttt ttcacatatt cagtatttat tcccactact tgagaaaaat actcaccaat
     1081 atccccacga ttccgccaat aatcagcgta gccggcacac cgtaagcatc tttttttgcc
     1141 agccgctccc actcgatacg tgtgggggtc atcttcgtat ggctcatgct ctgatagcag
     1201 aggtaaatat gtttccaacg gcacaggtag cccaccagat tatagatgcc atccaataat
     1261 attcccactg ccagtacaaa caacattgta attctccccc ggtagaaatc tgccagcgct
     1321 ccggctatca gaatcaatac cggatagcca taacgatcca aatatgccag aaggtacatt
     1381 actgtcgtag aataactgcg ctccggcttt ttctcaacga tcacttccgt acacccccag
     1441 ccgctctgca tcttgtaaga atctgacttc aatctctccg ggcgtatgct ccgccagatc
     1501 cggtaccgac aataacttcg gtacctctcg gttctcttct ccgtgaagca gcgtcttcag
     1561 ttccagttcc aggatctcat catcaatctg cttccaacga cagctctctt cttccgtcag
     1621 gtcatccagt ccgaaatgcg tgaggatcgt tcccataatc ttactttcaa tttccagata
     1681 attggacagg tatatcttca ccgggcgaat aatatcagaa atatatccct cactggcatc
     1741 atgaagcaga caggccagct gctgcctctc cgaccagcct ctggctttcg cttccgcagc
     1801 acagttgaga gaatgctgtc ccaccgaata aaaataggtc agctgtccac cgccccggca
     1861 cagtaagctc agcgcatggg cgatatcttc gatatagaca tctcccggtg tcatctgcat
     1921 gggatcgaat tttctgccgg aataggtcgt catggtgttc atagcttttg ctccttattt
     1981 ctccgcacgg atcaggttct tatagaatct gacagctccg ggcagacaat tatactcaat
     2041 attttcgttc aatccatgca ttcccttcat ctgctccggt ccatagatca ccggtgcaaa
     2101 acggatacag ctcttacaga taggctgata gaactgtgca tctgtcgcgc ctgtcataac
     2161 ataagggcta gaaggcagtc ccgggaaagt atctgcgatg gtctgctcca ccagatgaaa
     2221 tgcttctccg tgaatatcca caggcggcgt ataatcattg gcatgcagca cttcggtctc
     2281 cagtccgtac ttatcagcca cctttttgat gatctccagg ctttctttct ctccctgatg
     2341 aggaatgaat cgcatgttcg caccaacata agcctcctgc ggcagcacat tataggcatc
     2401 ggatcccttc tgtaccgtaa acgctatcgt ggtctttaac atcgctcctg cctgtgcact
     2461 gagcgatggc atcaccgctt ttaataaggg gccaaacagc cacagatttc caaataccag
     2521 tttcattcca aacccggcat aaggcgccag tgctgtgaac atggcggata cctccggcag
     2581 catctttttc cggaacggtg aatgcgtctc cacttcattg acgaatgccg acaatctggc
     2641 gataggggtt ccctttaccg gtgtgctggc atggccgccg ttgctccttg cggtaaattt
     2701 cacatccgcc ttgccttttt caaatactcc caccatggca aaattaccgt ggattccacc
     2761 gatgggctcc gtgatgatac caccgccctc atcacatacc aggaacagtt caatccccct
     2821 gcgctgcagc tccgctacga tcttcggtgc accgtcccca ccccactctt ccgtacagga
     2881 acttgccaga tagacatcag tgggtggtat gaagccctct gccaacaatt cttccactgc
     2941 ctggaaaaat gccatcacag agcatttggt atcggacgcg cctcgccccc atacctttcc
     3001 ttccgcaata tctccggaaa aaggtgcatg gatccactca ccctccgccg gcactacgtc
     3061 ctgatggctc atgagcagga tcggcttctc agagcttttt cccttccagt aaaataacag
     3121 gttgccgtca atctctgtct tctccagctt ctcatgtacc agcggaaaca attcctccaa
     3181 cactttgtgg aaccccagga acttctccac ctctgccacg ttggcatagg aggtggtgtc
     3241 atattggatc attttcgaca gcttctgtgc cagtgccagg gattcctcct ccggttcctc
     3301 tgccacgtag gaagaagtct tcccaggcat cagtaaggta cggatcaccg cgatcagtaa
     3361 caacaatata attgcgatga tcactgccag taaaatgaaa cctatatatt tcatatttcc
     3421 tcttttccgg gcgcttcctt ctattactca cataacctga caggtttggc cctttttccg
     3481 ttcatctgtt ttctgttcgt atagccttct gactattcat ccccggtatt tgccctgtcg
     3541 ggatcagctt ttgtctgttc cgggctctgc atattctttt tccggaacag cagatatgcc
     3601 acaccgatgg caatggcacc ggacggaaca atcataaagc tggtggagcc gatcagtgac
     3661 ggcaccagaa taaacagcag actcataagg atcagcggca atgcagcgat cttcatattt
     3721 ttccggtaat atttataagc catggctccg aacagcgccg gaaccacatt ttcaaaagcc
     3781 ggctgcaata cttcgctctg cagcacgggc tgcagcggca ccatcaacaa cacgcccagt
     3841 gccagaacga ggattgtcac cagagaagag gtagcaatag agattgtgga aatgatctca
     3901 ttctccgggg ttcctgtctt cgctcctacc atgtctctcg cattgaccgc acagggaatc
     3961 ttcatattga tcagattacc ggtgataaat gccaagtatc cgccgccggc tcccagcatc
     4021 ggcgtataga tcaggaattc cgcaatgctg ctgaccagcc atacgattcc cacggagagg
     4081 aagcccttgc cggcagcccc cagatccgga tacgcaccta aaagcttacc gatgagaaac
     4141 ggtgctccca ctaacattac taatgtgaca atgctgactg tccgtccgat gatatgtgtt
     4201 ttcttcgtat agatctgcca ggtatccgaa gtctgcccgg tctgctcctt tgttgcttct
     4261 acattattca gttccttatt atctattgtc atgctattcg ttttcatatt aagctccatt
     4321 ccgcagacga ttccttccgt tggaggaatt gcgaatcaaa ttttaaggtt gtcacgtcag
     4381 cctttacagc aatcccacca gtacggctgc tgtcatgccg atgatcatgc ttccggcaat
     4441 ggaaaagctg tccacccagg cttttccttt tttctcaatg aggtatacaa atcctgccat
     4501 caccagtgcc gataccgcca ttaccactaa cggaatgtaa tctccgttta acgtaaacaa
     4561 tccgttctcc gatacaaaac cgccgagata tcttccgatg taggtgctga ccagtccgat
     4621 gaacatggca gtcattgcca tatctccaaa tccaggctta tctgattttt tattcgaggt
     4681 caatttctgc gtatattttt tattcaggaa aatggacaat gtcatgcccc aaataatgca
     4741 gatgctcatg agcagtgcga tcgtggaaaa tgcctgcggt gtcatttccg cagcagacaa
     4801 tgtgctcatg cccacctgct ctgccgctgc ctgcgccacc tgggtctcat aatgcagggc
     4861 accgatcacg gataatcgaa gccacggcca gggcgttccc agacttcccg acagggcgat
     4921 gacacctaac agaatcccca cactgggcaa cagggaaaag gtcgcactgg agataatcgt
     4981 acgcttcatt ttcgtggtat ccataccaag tgcctgcccg gcgcagtaag ccctcaccag
     5041 aaaaatcaca cagaccaccg ccacaaaagc aatgatacct ccacagatca ggtacatcgg
     5101 tacactgttt aactggctta agattgacat ttctcttcct ccttcagaat ctctctgatt
     5161 ttatcttcct gtaaatgctc tccgatgacg atcagcacct cctgccccac tgcgacagac
     5221 tgcattgtat gactgtccgg tgtagcattc aattccatcc attgtcctgc tccgtttttc
     5281 acataaccct tgatccggaa tacttctccg caagacttgt cagaaaacag tctgggcacc
     5341 gcccgttcca aagcctccgg cttaatcttc tcttccatga aaaataaaga gtcgaagctc
     5401 tgtccattct ctaaatccat cttgcggtaa ctttccagac gataaccgca tttggatact
     5461 tcctctaaat cttcttttgt cagatccgtg agatttttct gtaaaatatc cttctcaggg
     5521 tcgattctcc ggtcacagcg gatctgttcc agtgcccggt tgagatgtgc caccgtgttc
     5581 tcacattgtt ccctggtggc tgcatccgca tggctcagga tcacttttcc ggcattggca
     5641 gcctccgatg ccagcagata gtctgcctgc tgtgagagat tatcctccag tcttgcatcc
     5701 agtatggtga tgacattgcc gatctcatac cactggtcca gcggctcctc ccgaagtaca
     5761 tcaaaaaact catctacatc atagattccc gacggctcca ccagcaccct gtcataacca
     5821 cacattcgca tggagatcag cttcgtacgg aaacgcctcc tatggctctc cgcatcgcat
     5881 cctccggcga tcatctccag ttcgcactga tctcccctga gttcgtggag catcagcata
     5941 tccacgttga tggcaccgaa atcattttcc agaatgccga tatgcagtcc ctgatccatc
     6001 aggtactctg cgtatttttt cagaaaagtg gtctttccgg atcccaaaaa gcccgtgatc
     6061 agatcgatca ttaccatata tcgtctttcc tctctgtttc attgatttgc atagtgatca
     6121 cttctataat tctttctcat aacaccagta ggaatgtccg aaaagctcac attcccctgc
     6181 tgtggtaaaa tgcaattttt cataagagcg caatgctttc acattgtcct ttgccactaa
     6241 aatgtgtacc gccttataac cctgtgattt caattccgtc atagcttcct gcaacaggat
     6301 cctggcaatg ccacgattct gatattctgc tgccactccc agtctggaca gctctgccac
     6361 cggggtaagg tcctctgacc aacaggacag tgcctccact tccgggtcat cgtctatgga
     6421 gattgtgcct attaagcgtc ccgagggctt ctcccgcaga cagaacagtg catccctgga
     6481 caggtcaaat tccacttccc gttccgttgg gtaattttct gtccagacac aatattcggt
     6541 tccgatcagg gagcggtata attgcaggat ctcttctgct tcttctctgc atgccctgcg
     6601 aaattccata taacaatcct tcatgctgtt ccatcccttt actgacttat ctttttcgtt
     6661 tttatgcttt acgttttttc atttgcagct tgattgcttt actgttttat agttctccgc
     6721 tgccgccaca tacagtacac tttttgtctc cgtgacacag aatgcagtca tcagagatca
     6781 cacccttacc atgacagata ctgcaagact taccaccgct tccaccgcac tgctggcagg
     6841 tatttccgcc actgcctcca caatgccggc agctggttcc gctggtagca tccacaccgc
     6901 tgccgccaca tccgggacag gtctgtctgc cactgcctcc gcaggtaccg caggtctccc
     6961 acaaggatcc tccacaacgc ttacaaggtg ctgtaccacc cttgcattcc tggcagattc
     7021 cggttccgtc acaggcactg cattttgtct tctgtgaaac atttgttgcc aatccattac
     7081 ctgcatcgtt ttccggaact gcattgtttg ctccgctttc cgccgcattt ccagccttat
     7141 ctgaattagc tgaattagct gaattagctg aattacctgt attacctgta ttatctgtct
     7201 tatccgaaac agtatccccg gctcctgtgt cggttccttc gatgatgacc ggcgtaccgg
     7261 catcctgcag ctctaagaca gcatccacac cttgttcttt taattccgca gtaactgcag
     7321 ccgcgatcac ctgttccact gtgtctacct gcccggactc cgttgatttt tccagatcta
     7381 tggtaatatt tttaccgtct gccagatact cttttgccac tgcctgctgt atgatcacag
     7441 gcatagcctt ttccagatcc atccccgtga ggtccgtctc tgagaacaga tcttccgcat
     7501 cctggttcag acatgtaacc gccactacct ttgactgctt atccagatat aacctgaatt
     7561 cgggattgat catgatatgt agccggtatg cataatccaa tgtttccgga tctgttgtct
     7621 ctgcctgctc ttctgccgca gcagtctgtt ctgtggcagt tacagcgatg tctgtctgta
     7681 cctgtgatcc ttccgcttcc tgtgctttat tgccgcagcc agataacagc aacaaactta
     7741 ttcccgttaa caacgccgct attttgattc tttgacctga taacacttct gccttgtttc
     7801 tctttttcat atcctacctc atagctttct tgcatctgtt ttacaccttt aatgttttca
     7861 gatactcttt ctgcccccga gtgatccggt agctttccct cgccttctgt atggtcttgt
     7921 tatgagtcca tggatccaag cattttttct cgatatagcc aattgccgca tcccattgtt
     7981 ttgccagtgc cgtggcataa aaccaggcaa tcatcatgtt cacataatat tccccggaat
     8041 gtaccgctgc cgggatctcc aaatattcct cccggaaatc ctcatccagg aaatgtgtca
     8101 tcagcatttc cattccgaag cggcaggtat atgggtgttc cgaagctgtc cattctctta
     8161 ttttgtctat aagtttgtct ttgtttttgc ggaacacctt tggggaaaaa atatcacaca
     8221 ccgcccaatt atccacataa ggcaggaagc gctccacttc ttttacacag gcttcgtaat
     8281 ctttgatctc cgccaccaac aacgcatgca aaatattttc atcataataa gtgtgcggca
     8341 gctgtttcag aaactctacg ctctccggat cctttccata ctctttcgta agcttacgaa
     8401 ttgcgggaat acggactccg ataatcgtct ccacaggaat cgtcgggatt aatttgctct
     8461 ggaaatcacg gtaaccttta tcttgcaatt gaaataataa ttcctgtgct ttcatacaat
     8521 tttctcctca aaatgcctgc tttctgttta ttgtaccaca agcatagaag atatgcaaaa
     8581 cagtcatgga tggaaattga gagtggggag gcgaacgtcc gcctgtgccc ttcttccgca
     8641 tcagcgtggg cacaggagat aggaacaagc tggtctgtac ccttcttccg cctcaacgtg
     8701 aatagtttgt gggtataccc tgtcaatgtt tccttggttt taataactgg taatccatct
     8761 ttttctgaac ataattcctt ataagtctct atagataata atggaaccct aataataaac
     8821 gtctttccta cttcatatgg catcatatca attcctccta atttgctttt actttatttc
     8881 gtaactcttt tattactccg acacttga
//
LOCUS       DAWDHD010000102         8842 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_469_length_8842_cov_9.405485, whole genome shotgun sequence.
ACCESSION   DAWDHD010000102 DAWDHD010000000
VERSION     DAWDHD010000102.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 8842)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8842)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8842
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_469_length_8842_cov_9.405485"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1899 a         2439 c         2033 g         2471 t
ORIGIN      
        1 aacatcatgt tcgtagacgg cggtactgac gcactgttaa attctgaaaa ggtaaattaa
       61 ggaggaacac gctatgagac ccattgaaaa atatgtagaa gctatggaaa ataaagattt
      121 tgccggactt gcagagttgt tcacccctga tggcattctg tgtgactatt gtaccacctc
      181 cgcttcccag caggaatatc atatctacgg aaaagaggcc atcaatatgt tcttccgtaa
      241 caaattcggc ttccgccgct actccattct ggatgctgag gtcgtaaatg atgagcaggc
      301 cgagttcatt gccaacttcg gaggctacaa catcatggcc atcgccacgg ttcgtaagac
      361 cgatgaaaac ggtctgatcc agagacttac cgtaagaccg aaataatttt tgccatcccc
      421 ttatactttt ctctatatag aatcttaatg taaaagaagc tgtaccgtag ggtgcagctt
      481 cttttacatt ctatcttccg aatacgtcgg tcattccgga gcactgccag agatcacata
      541 gctggtcacg gcatacagga tctcaccgtt gtactccact ctggaccagc cagatccttc
      601 actgtagccg gtacgatgca gattggttcc gttccgcacc tgggtgcgta cagtgctctc
      661 tccctcgctg gtgcttggtt ccgtccgcag attgatcaga tctttggggg taatataatc
      721 atcacaatcc gtaaagacga tcactctgcc gtcctgggtg ctgacgcggt tgggatatgt
      781 cggcttctcc ggggtcttat aggtaagatc cgtggtcaga tatgctgata ctgcataaac
      841 cgtctgaccg ttgtattcca gtctggacca tccggtggaa tcgttccttc cggtgcgtac
      901 cagagtctct ccgttcttca gctgccctac cacattgccc tcatctccgg tatagggtgt
      961 actgcgaaga ttactcacat ccttggcagt gaccgtctcg ttcacatcac cgaacgtcat
     1021 ggaatttccc tccgcaagag catctcccgt attattggta gcggggactt cggttgccac
     1081 cggtgcctct gtcggctgcc gctgtgcttc cggagcctgt gtaggtgtcg gttgcggtgc
     1141 ttctgtcact gctgccgcag gctccggagt aaccgtctcg gggatttgtg tagtctccgg
     1201 ttccgccgtt gccacctttt ccgatgcgat cccggtctcc ggggtctctg cagaagcctg
     1261 ttctgagctc tgcgcttctg ttgtcaccgc cgcgaattca ttattttccg tagttctgtc
     1321 atgatggctc agtttccaca gcggaataca gatcaggacc gccagaacca tcatgcccgc
     1381 aaaagccgga atcatccatg gccggaaccg gaagccgatc ccgtccccct cgtcgaagtc
     1441 atccaggtca tccccttccg ggtactcccc atagtcctca tcctccgtaa agtcctccga
     1501 ttccacagta tacggatcct ctgtatcaca ggttgtttcc catacagcat catcacgtcg
     1561 tcccgcctgt tcagaatcca cctctgcatc cgcaagttct tcatagtcaa aattatattc
     1621 ttccggttcc cttttccggt ctctgacacc cttttcttcc atggcatgcc ctccttccgc
     1681 catctttaag ctgattatat acttccctct tttgcaaaac aagtaccttt tcctgtcttt
     1741 gcaggtatgt tatactgact gtagcattat aaaaacctct caggagttat attctctatg
     1801 aaaaataaac ttgccaaatg gaaaccttat attttactcg cggtcctgct cgccagcctg
     1861 gttcctctgg tatggctggg gcgttacaat tatcccaccg gggatgatta ttattacggt
     1921 acggaagccc atctggtctg gcagcagacc ggaagcatac cacaggccat ttccgccgcc
     1981 tgtgccggtg tcgccaaaag ctatcagatc tggcagggaa cctattcagc gctgtttctg
     2041 atgtatcttg cgcccaatgc cttctccaat accgcctatc atctggtgac ttttgtgatc
     2101 cttcttttgc tgtgcggcgg cattttctat ctgctacggt ctctggtctg ccattttctg
     2161 ccgggaacgt gtggggaatg gatcacgatt tcctcagtat ttagtttcct gtgcatccag
     2221 acagtggcct ttcagagcga cagcttttac tggtacaacg gctccatgta ttacaccggt
     2281 ttctttgccg tgaccctgtt tttcctgggc acattgcttc gctacctgta caatgggaaa
     2341 aagatactga tgcttccgct tctcctgttc gcaattttct taggcggtgg caattacgtg
     2401 tcccttctcc cctgcatgct gtttgtggta accgtaacct ttctactgct gttgcagaga
     2461 aatagaaaga cttatgtctg cggaattacc tccgctgttc tcctgctatc ttttgccgtc
     2521 agtgccatgg cccccggcaa tcaggtgcgg caggacggca tgtggaaaat tcccgcgtgg
     2581 aaagccattg caaagtgcct gttgcagggt gtccgctata ccttcgcctg gaccggaccg
     2641 tggtggctgc ttgccgccct gcttctgctg ccggtcttcc tgcgcatttt acagaagaaa
     2701 aacggagggt ttttctccca tcccctcctt tttaccggat attcctacgg attgttctgc
     2761 tccatgtcct gtcctctgtt ctataccatg aactccacag gacccggccg ggccgtagcc
     2821 attgtctact atacgtttct gctgatcagt tttgctgtgt tcttctactg gctcggatat
     2881 atagtccgga agctgcaaat gcgtcagaat actccggaga aaatggcggt cctcggcaag
     2941 ttgaaaattg cccgttatgt cgccatgacg gtcctgttgg ccgtcatcct tttcaccgga
     3001 gtatggcagg agacctccgc agcgaaagcg gtccaggtgc tggcagacgg ggaagccgct
     3061 gcctatgctg cagaatacga agaacgtctt cttctcctga atgacccgga gattacggat
     3121 gtggtgctga ccccctttac ccatcagcct gccatgatct ataccggtga tctccccgga
     3181 gacccggagg atcccaccag taaaaagacc gcacaatact ttggaaaaaa ttccatatac
     3241 gtagattaca gcaattaatc ccccaaaaaa ggacgcttcc ggtttttccg gaaacgtcct
     3301 tttgttatct tttactattc ttattgtcct tcgtacttct cactctgcat acggatcagt
     3361 tctgcatcgt caaagtagtt gatgcgcatg gcattcttaa cctcagacag ggtctgcgct
     3421 gccacttcac gggcctcgtc actgcccttc ttcaggatct catatactgc gggaatatcc
     3481 ttctccagtt cttttctacg gttacggatg ggctccaagg tctcctgcat tacgttgttc
     3541 aggaactttt tcaccttcac atcacccagg ccgcctctgg tataatgagc cttcagttcg
     3601 tccagatttt cataatccgg caggtatctc tcgaagtgct ccggctggca gaaagcatcc
     3661 aggtaagtga ataccgtgtt gccctccaga tgtccgggat cgctgaccaa cagatgctcg
     3721 ggatcggtgt acatgctcat gatcttggtg cgtacatcgt ctgcactgtc tgacagatag
     3781 atgcagttgc ccagggattt actcattttc gccttgccgt cgatgccggg caggcgctgg
     3841 caggcctcat tttcgggcag caatgcctta ggctctacca gtaccggtgc atatacggtg
     3901 ttgaatttgt gtacgatctc tctggtctgt tcgatcatgg gcagctggtc accacctacg
     3961 ggaacggtgg ttgccttgaa agcggtaata tcggaagcct ggctgatggg ataggtaaaa
     4021 aatcccacag ggatgctggc ttcgaagtta cgcatctgaa tctcactctt tacggtcggg
     4081 tttctctgta atctggctac ggttaccaaa tccatgtaat agaaggtcag ctcgcacaat
     4141 tcggagatct gagactgaat gaagatcgtt gccttgctgg gatccagtcc gcaggacatg
     4201 tagtccagcg ccacttcaat aatattctgg cgcaccttct cgggattctc gatattatcc
     4261 gtcagcgcct gtgcgtctgc gatcatgata aaagtcttct tatattcgcc ggaattctgc
     4321 agctctacac gtcttctcag ggagcctgca taatgtccca catgtaatct tccggtgggt
     4381 ctgtcacctg tcaaaataat cttgtccatt tcgtcctctc aatccgccgt ctatgcggct
     4441 atacgtcatt gcttctatca taccatcttt ttgcatagaa gcaaatgatt tttctacaga
     4501 attaacgctt tgtgtgcaag ccccctcgca ttcattctgc tgttctatgg ctgaactatt
     4561 gcaagcagct tatccagaat tttatgtgcc acctgttcct tgctcatcag ttccagactc
     4621 tcctcggaat tctttgtgat cagtgtcaca atattggtgt ccgtaccaaa gcccgctcct
     4681 gcgctcttca aactgttggc acagatcatg tcacagtgct tgctctccag cttcttccgg
     4741 gaattctcca gcacattttc cgtctccatg gaaaatccac acaaaatctg tcccggtctc
     4801 ttgttttccc caagggcctt caaaatatcc ttcgtccgca ccagagggat cgacatgtct
     4861 ccgtcttttt tcttaatctt attgtctgct gtcactgccg gggtgtagtc tgccacggct
     4921 gctgccttga tgacaaaatc atagtcctgc gctctggaca cgattgcatc gtacatctct
     4981 tctgccgtct gtaccggcac cacatttaca aacaaaggcg gctccacatc cgtatgggct
     5041 gccaccaccg tcacttctgc gccgcggtgc attgcagctc tcgccagggc ataacccatt
     5101 tttccactgg agtgattcgt gataaaacgc accgggtcaa tgggttctct ggtggcacct
     5161 gcggagatca gcaccttttt gcccgccaga tccttctcgc aggtgatctc cttcaccacg
     5221 gcttcgaaca gtacggactc ctccggcatt ctgccgtcac cgatatcgcc gttggccagc
     5281 attcccgtgg ccggagtgat gatctcatag ccgaaatgct ttaatttttc gatattatcc
     5341 tgcacgatgg ggttgtgata catattatga ttcatggcag gggacacaat cttcttgcag
     5401 ggacatgcca tgatcgtagt agtcaacata tcatctgcga tgccgtttgc aatcttgccg
     5461 atcacattgg cagaagccgg tgccaccaga accacatccg cccgctttgc cagtgccaca
     5521 tgttccacac tgtactggaa attgcggtca aaggtgtcca ccagacattt atgtcccgtc
     5581 agactttcaa aagtaatggg gttaatgaac tgcgtggcat tctgtgtcat cagcacttcc
     5641 acatcacagt gctgttttaa cagcatccgc gctacattgg ccattttata tgcagcaata
     5701 cttcccgaga ttgccagaac tacggtcttt cccttcagca tagtcgtctc cttttgcctg
     5761 atttatcaaa aatacttcaa aactgtactc ctaaaatacc ataagtgcat attttatgca
     5821 aatatcactt ttctttttgt ccgggatatg atataaacag taggagtgtc ccgaatcacc
     5881 ggggcagctt gtattgtatc tcccagaggg agaatggtaa caaggaggaa aatttcatat
     5941 gaacaaacaa agtaagacaa tgcgactcac gttgatggga ttgtttgctg cggtactttt
     6001 gctgatggct tataccccgc tcgggtacct gaacatcggc cccctggcaa tcaccctcaa
     6061 tgtcatcccc gttgcactgg ctgcgatcac actgggccct gtaggcggag ccgccatggg
     6121 agctgttttc ggattgacca gctttttgca atgcatcggc gtgggaggca ccagcgccat
     6181 gggagcaatg ctttttggca tcaaccctgt gctggccttc atccagcgtt tcatccccag
     6241 acttctggac ggatttctgt tgggatatgt cttccgttac agccgcaaag ccgtaggaga
     6301 cagaatcgcc tgcatgatca ccggattttg cagcgcattt ttcaacaccg catttttcat
     6361 gtccctttta gtactgctgt tcggcaatac ttcctatgtg cagggactga tgggtggtaa
     6421 aaacgcgatt ttattcatct gctcttttgt aggtgtgaat gcggtcttcg agatgtgtgc
     6481 atccacgttc ctgaccggag cggtagggct ggcactgatc aaggcaaaag tcattcctgt
     6541 atatcagaaa gcagagtctg cagcataaca ggagtatacc cgtggtactg acaatcgaca
     6601 tcggaaatac tacgatcggc atcgggggat ttcagggaaa taagctgctc tttattgaga
     6661 agatatcgtc ccgccgtccc ctgtctgatg ccggatatag aaaaaaactg aatatgattt
     6721 tagacaaaca cggtctgtcc gccgaagcct ttgagggatc tgtgatctcc tcggttgtgc
     6781 cttcggtgac agccgttttt tcctctgctc tggaagcttt tctggggcag aagcctctgc
     6841 ttcttaccca tgaactggat cttggctatg agatctgtac ggaatttccg gaaaaggtgg
     6901 gctgcgaccg cctggcagat atcgccggag ccatttcccg ctatcctctg ccactgatca
     6961 ccattgatct gggcaccgcc acgacattta acgtgttgga tgagaaccgt cgctttttag
     7021 gcggcctgat ccttcccggc gttggaaccg cccacagggc acttttaggc aggaccgccc
     7081 agctcccgaa aggggacatt catatgcccg cccacaccat cggtgcgaat acgcaggaat
     7141 gcatcaccgg gggaatgctc ttgggcaacg cctgtgccat cgacggactg atccgccgta
     7201 tccagacaga gttagggcaa aaagcaacgg tcgttgccac cggagggtac gcaacgaccg
     7261 tcattccaca ttgtgaaacg aagatccata tcgatcagaa tcttcttatg aaaggtctga
     7321 gatccatcta cgagaataat acctctactg gatcaccaga ttagagtagc ccatcaggct
     7381 ctcatccaca gcatgtgcag cttctctgcc ttcacggatc gcccacacca ccagggactg
     7441 tcctctgtgc atatcaccgg tgacgaatac attttccaca ctggtctggt atttgccggg
     7501 ggcagttttt acgttggtgc gttcatttaa ttccaccccg aaagcatcgg taatatattt
     7561 ctgacttccc aaaaatcctg ctgcgatcag tacgatctcc gcatccagga tctgctcact
     7621 tccggcgatc tcctgcatct tcatgcgtcc ggtagccgga tccttttccc aggacagctt
     7681 cacgatcttc acggctttca ggttaccgtt cttatccttg ataaattcct tgaccgtaga
     7741 ttcatagatt ctcggatcat gtccgtagac tgcaatggct tcttcctgac cgtaatcggt
     7801 cttgcagacc ttcggccatt cgggccaggg attgttctct gctctcttgt ccggagcctt
     7861 gggcatcatc tcgatctggg ttacagactt ggcaccgtga cggatggaag ttcccacaca
     7921 gtcattaccg gtatcaccac cgccgatgat gaccacgttc ttacccttgg tctccacata
     7981 ttttttatct tcaaagttcg aatccagcag gctcttggta ttggctttca gaaaatccac
     8041 cgcgaaatag attcccctgg catctctgcc tggcgcattg atgtctctgg gattggaagc
     8101 accacaggcc agtacgacac ggtcaaaatc atgtaacagc ttggatgcct tgatgtcctt
     8161 accgatatcc gtaccggtga caaaggtgac gccctccttc ttcataatgg ctatcttacg
     8221 gtcaatgatc tgtttctcca gcttcatgtt ggggatgccg tacattaaca gtcctcccac
     8281 acggtcggag cgctcaaata ctgtgacctg atgtcctctc tggttcagca gatccgccgc
     8341 tgccagtccg gaaggaccgc tgccgatgac tgccaccttt ttgccggtac gcaccacagg
     8401 gggcttggct gccgcataac cctcgctgta ggcgttttca ataattgcat attcgttctc
     8461 cttggtggag accggatccc cattcaatcc acaggtacat gccgcctcac acagagccgg
     8521 acatactctg gacgtgaatt cggggaaatt gttggtcttc ttcaggcggt tgtaagcctg
     8581 ttgccagttg ccggtatata ccagatcgtt ccactccggc accagattgt gcaggggaca
     8641 tccgctggcc ataccgcaga tcatcatgcc cgactggcag aacggcactc cgcattccat
     8701 acatctggca ccctgtaagc gctgcttttc cttcggcaga tgctcatgga attcgttaaa
     8761 atgctgtatt ctcttcttag gatcctcagc cacgctgact tcccttttat attccataaa
     8821 tcccgttggt tttcccatga ct
//
LOCUS       DAWDHD010000103         8745 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_473_length_8745_cov_11.540621, whole genome shotgun sequence.
ACCESSION   DAWDHD010000103 DAWDHD010000000
VERSION     DAWDHD010000103.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 8745)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8745)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8745
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_473_length_8745_cov_11.540621"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2469 a         1882 c         2401 g         1993 t
ORIGIN      
        1 tctatgactc caacaatttt cgttctgaca gggtactcgg caacggagtt ctgcggctgg
       61 acaatgccta tgaaggacaa aagggcagct ggctgtctac ctgtggaaat gtctatgatt
      121 attttggcga ttacgagatc gaccaggccc tgtacgcagt ggatgattac atagacgaaa
      181 gcccctacaa agcgtataag aattgtatca gttatgtgac ccggaccatg gaggaaagta
      241 aggaaagcat gccgatgacc ttttctccct ggatcctgac aggtcttgtc gttgcactga
      301 tctatgccgc agtgaacctg catcagagca aagcaaagga tactactacg gtcaatcagt
      361 atcttgatgg caaaaagccc aagatcaaca ataccagaga ccagtatctg cgtaagaacg
      421 tggtgacccg caggatcgag accagcagta gcagtagcgg acacagcggc ggacacagcg
      481 gcggtcacgg aggaagtcat cggagcagca gcggtgtcag ccatggcggc ggtggccata
      541 gaagataaaa taacaagttc cattcctgga acaagagatc cggcgtatcg ggtgcagaca
      601 gacactcata cgccggtttt ttcgttttac ggaggaagga gcgacggcgg gaaacagtag
      661 tggaaaaatt ttccatgggt tgacaggaag cctgcagaca ggtataatca gacttgcaat
      721 gtaacaattc tgtaatatat ttgtaaaagg attttaaaga agaatttatg ataaaacata
      781 caagagaaaa acaagtaaag atcgtagcca ctggcctggc attgtgtatg ctggcggcac
      841 cgctttccgc ttccgctgca gaaagcagtg tcctcatggc atccggcggt gtggcttctg
      901 tcctggagac ggagcgtaca ctggaggaat acatccagat cgcacaggat gcccagggag
      961 cttcctgggg ctataccaat atcggtgtgg ccaatgtgga atccggtaat ctgaatgtga
     1021 gagcggtatc ttccacagac ggtaagctgg taggtaagct tcccaaggat gcggcctgtg
     1081 aagtggtcga gaccgtagat gactgggcac atatcaagtc cggtgaagtg gaaggttatg
     1141 tcagcaagga attcctgctg actggaccgg aagccagaat ccgagccatg gaactggttc
     1201 atacggtagc cactgccaat accgatggcc tgaatgtacg tcaggagccc aataccacca
     1261 gtgagatcgt gactcaggtg ggacagggag aagagatgga gtatgtggag accggttccg
     1321 atggctgggt gaagatttct attgatggcg aagatgctta tgtatcccaa gattatgtca
     1381 cagtggaaga aaagctggat accgccatca ccatgacaga gcttctctac ggacaggggg
     1441 tatccgatgt ccgcgtagat ctggtagaat atgcaaaaca gttcgtaggt aacccttatg
     1501 tctggggagg caccagcctt accaagggtg cggactgctc cggtttcgtt ctcagcgtat
     1561 tcaagaaata cggcattacc ctgagccatt cctccagagc acaggccaat gagggcacca
     1621 agatcagtgc atccgagcta aagcccggtg atctggtatt ctatggtaac ggcaagggta
     1681 atattaatca cgtagctatt tatatcggtg gaggacaggt aattcacgcc agcagcccga
     1741 agaccggtat caagatcagc agctataagt atcgtacacc tgtgaaatgt gtccgtgtca
     1801 ttcacgactg ataaaaagca ttgctttcgc aaaagggcgg ggcaatgctt ttttgtgtac
     1861 aaatcgagta ggagacagcc tgcttgatta ttcgaagata tgcgcaggat ctttctttta
     1921 caattgcgtt tcacggggaa ctatgatact atttttgtat atatcggcgg tccctgaacg
     1981 aaaaaaaggg gcttcgtaca gagacgaaaa tgggtgagga atatgcggaa agtagctttg
     2041 atcaccgacg gctggagaag gttgtttacc tatgcctggc cggcaggtat tttacagaga
     2101 ataaaagaaa cgaacgaaga ggtaaatcta tatattttca atagtaccgg caactggaac
     2161 agagatgccg gatataacag ggcagaatat aatattttta accttccgga tctgtcggag
     2221 ttcgacggta ttcttctgga actgaataat atcagttccc cggcagtgct ggcggaagtg
     2281 atccacaagg cgaagcagtc gcaccttccg gtagtctcta ttgccaacga gctggaagat
     2341 ttttattatg tgggtattga caattattct gccatgaagc aggtgatcgc acatatgcat
     2401 gaggtgcata actgcaaaaa attctggctg ctggtggggg ctgacagcaa ttatgagagc
     2461 agctgccgac tgcagggaat gattgactat gccaaagaac acaagatcaa aatagataag
     2521 aatgacatct ggtacggctc ttttgactac aagagcggat tggaaggcta caggcatctg
     2581 tgggatactc acaaagagct gccggatgct attatctgta tcaatgataa tgtggcggta
     2641 gcggtatgcg aagcggcggc ccagatggga tttacagcac cgaaggactt ccgtatcaca
     2701 ggctttgaca acttcgacaa ggccggattt tacacgccga gtattaccac tgtgggacat
     2761 atcagagagg aagctgcata caagggaatg gatattctcc tgaaaatctg ggcgggagaa
     2821 agtgtccccc ggtacaattt caccaatact gagatgctgt ggcaggagag ctgtggctgc
     2881 ggcaaaggaa gcagcaggga tgccagagcc catctgaagg atcagatcat gtacagcgtg
     2941 gaaagtgagg aattcgacga ggaagtcctg tccatggagg cagagatgat gcagtgcaat
     3001 accgtagagg aaatgatgta ctgcattccc cagtgcattc cgtccctgaa atgcgatgcc
     3061 atgtatctgg tgctggacga tcacctcaat gcctataaaa aagaaacgga gaagagtatt
     3121 catctggatg ccaccccttc ggacgaggga ttttccttgc atggttatcc gagacagatg
     3181 cagatgacct ttgcatacga gagggaccag agactggatc tggacagaca ggaagtggat
     3241 ggcatcttcc cgaccttcga ttatccgaag ccgggacagg actttttgtt cctgccgtta
     3301 catttcggag agcgtaccgt tggctatgta gctatccgta atgcggtata cctgatggag
     3361 aaacagtacc tgttccagat catgaacgca ctgacccgct ccatggagaa ccttcacaaa
     3421 aaagaaatgt tggcctatat gaaccgcaag ctgtcttccc tgtatattat ggatacgctg
     3481 acaggtatgt ataaccgtat gggctaccaa cagctgggag aggagtcctt ccgtatttcc
     3541 ggtctgaaca aacgcaaact tctgatcttg ttcgtggatc tggatcgtct gaaatacatc
     3601 aatgacacct tcggacacga gtatggggat atggccatct gcgctgcagc cagagcattg
     3661 atgcagtgta gcagccggga cgctgtgccg gccagaaccg gcggtgatga attcgtcctg
     3721 atccagagtt accagtccga tgaggtttcc agagaactgg tgctgcgcat ccgcagaaca
     3781 ctggaggcgg agggaaaagc ccagaagctt ccgttcccgt tgtccatgag tatcggtacc
     3841 gtggtgacgg atccggaatc cgcactgtct tttgcagatt atgtaaaaat tgcagacagc
     3901 cggatgtacg aggaaaaggt acagaagcgg gccgcaagaa aatagaaaaa tatatttaca
     3961 accgatagaa actatgatat attgaaacag tatgattagc cgatgcccag attacggaca
     4021 ctccgacctt agtcttggta tctggcgttt aaaaaacagg aggaaaaaga aatgatttct
     4081 aaggaaaaga aaacagccat catgaacgag tatgcaagaa cccccggtga caccggttca
     4141 cctgaggtac aggtagcggt cctgactgca agaatccagg agcttaccga gcatctgaag
     4201 gagaacccca aggatcatca ttctcgtcgt ggtatgctga agatggtagg tcagagacgt
     4261 ggtcttctgg agtacctgaa gaagaaggat attgagagat atcgtgcatt aatcgagaag
     4321 cttggcttaa gaaaataatg ctgactggtg agcggggtct gccgattaca tcggcgctcc
     4381 gcttctcttg tttctcatta catccatatg cacggtgaaa atctaattca ccgcgcctga
     4441 aaaggtaaca cacgggatgg aagtgagccc cgagaccgct gaaaagatta tgttgcggat
     4501 catgggagcg acatggaatt ttcagtagtt tcggtaatcc tccagcccgt agaataataa
     4561 ttcatatcgt agcgtggcac caaagataac agtcacagta cgatggatag gaggaattta
     4621 tgtatcaaag ttacaagatg gatcttgcag gcagacctct ggtggttgat atcaaccgtg
     4681 taggtaagca ggcaaacgga tgtgcattca tgcactatgg tgataccacg gttctctgta
     4741 ccgcaaccgc ttccgagaag cccagagacg gtatcgattt cttccctctg agtgtagagt
     4801 atgaagagaa gatgtacgct gtgggtaaga tccccggcgg tttcaacaag agagaaggaa
     4861 aggcaagtga gaatgctatt ctgaccagcc gtgtcatcga cagacccatg cgtcccctgt
     4921 tccccaagga ttaccgtaat gatgtaaccc tgaacaacat ggtaatgagc gtggatcccg
     4981 agtgtcgtcc ggagttggtg gctatgatcg gtgcagctat cgctacctgc atttccgata
     5041 ttcctttcga tggcccctgc gctatgaccc aggtaggcat gatcgatggc gagttcatca
     5101 tcaatcccag ccaggagcag tggaagaaag gtgatctgaa cctgaccgtt gcttccacca
     5161 gagaaaaggt gatcatgatc gaggcaggag cgaacgagat tcctgaagca actatgatcg
     5221 aagcaattta taaggctcac gaagtgaacc agaccatcat tgctttcatc gacaagattg
     5281 tagcagaagt aggcaagaag aagcatgagt acaccagctg tgcagtgccc gctgaaatgt
     5341 tcgaggagat gaagaagatc gtatctcccg ctgagatgga agaagctgta ttcaccgatg
     5401 ataagcagac ccgtgaggag aatatccgtg tgatcaccga caggctgacc gaggctttcg
     5461 ctgagaacga agagtggctg gctatcttag gtgaggcagt ataccagtat gagaagaaga
     5521 ccgttcgtaa gatgatcctg aaagatcaca agcgtcccga tggacgtgca atcacacaga
     5581 tccgtcctct ggcagcagaa gtagacatca tcccccgtgt acatggttcc gctatgttca
     5641 cccgtggaca gacccagatc tgtaacgtat gtactctggc acctctgtcc gagcagcaga
     5701 agatcgatgg cctggatgag aacgaagtaa gcaagagata tatgcatcac tataacttcc
     5761 cttcctattc cgtaggtgag accaagcctt cccgcggacc cggaagacgt gagatcggtc
     5821 acggcgctct ggctgagaga gccctgatcc ccgtactgcc cagtgaggac gagttccctt
     5881 atgcaattcg taccgtatct gagacttttg aatccaacgg atctacctcc atggcatcca
     5941 cctgtgcttc ctgcatgtcc ctgatggcag caggcgtacc catcaagaag atggtagcag
     6001 gtatttcctg tggtctggta accggcgaga ccgatgacga tttcgttctg ctgaccgata
     6061 tccagggact ggaagacttc ttcggagata tggactttaa ggtaaccggt actaccgaag
     6121 gtattaccgc gatccagatg gatattaaga tccacggtct gaccagacct atcgtagaag
     6181 gcgctattgc gagatgccgt gatgcaagac tgttcatcat ggatacctgc atgaagcccg
     6241 ctatctctgc tcccagaaca gaggttggca agtacgctcc caagatcatc tccattcaga
     6301 tcgatcccga cagaatcggt gatgtagtag gacagcgtgg taagaccatc aatgagatca
     6361 ttgcccgtac cggtgtgaag atcgatatca ccgatgatgg taaggtatct atctgcggta
     6421 ctgacaagga aatgatggat aaggctctgg agatggtgaa gatcatcacc accgatttcg
     6481 aagagggcca gatcttcaag ggtaaggtag tcagcatcaa ggagttcggt gcattcatcg
     6541 agttcgcacc cggcaaggaa ggcatggtac acatctccaa gatcgccaag gagagaatca
     6601 accgtgtaga agatgttctg actctgggcg atgtagtgac tgtagtatgc ctgggtaagg
     6661 ataagatggg cagaatcagc ttctccatga aggatgtagc acagcagtaa gctgtaagaa
     6721 cgcaaggcat aagaatataa gatataaaat aagaaaggat cgctgcatag tgtactgtgc
     6781 agaggtcctt tttgtgtata cgatgagcca ggtacagatt tgtgcttgca caagaatcgg
     6841 tatttggcaa atgtacatca tcgagctttt aagcgagatg gtgtataaag tatatgaaac
     6901 gggcggaaga cagtcagccg gatctgcctg gaatgaggca gcaagaagtg acatttgaaa
     6961 aagggcacaa aaaaagcacc tacatgacgg tataggcgct tttgtatgtc ttaaattctg
     7021 taccagtgca agaaaaatta agccattccg ttgacagctt tgctcagacg ggaaaccttt
     7081 cttgcagcgt tgttcttgtg gtaaacgccc ttagaagtag ccatctcgat ggtcttggta
     7141 gcagcggtta attcagcctt agctgcttcc ttgtcaccgg tctcgattgc agcgtatacc
     7201 ttcttgatag cggtcttaac gccggatctg atagctttgt ttctgtcagc tcttgtgcgg
     7261 gatactaaaa ttctcttttt tgcagattta atgttagcca agtcgtacac ctccataatg
     7321 tatacaataa aattataatt tacaagaaat gtttcacttt agccagacac ggaagactaa
     7381 cgtaaacata ctattgtagt ttagacaatc tgggagcttt tgtcaataca tattttgcgg
     7441 atttttgtaa aaaatgtatt gataagaaag gcagacagag ggtgaaaaaa tggaaacttt
     7501 tcagatcaga acagacctgg ccctggaggc aagagaaagt gtcaatgaag aggaaagcaa
     7561 actgcggggt gtgtcggtgg acgagcacta tgaggaagag acggatatcc gcattaccaa
     7621 ggtgaccatc gatacgaaaa atgcggaaaa aacactggga aagcccatgg gtgtctatgt
     7681 gaccatggag gcaccggcca tggtggaacc ggatgacgat taccacaggg agatctcgga
     7741 ggcgttagcg gaggaacttt tgaaaatgat gcccggggaa gcggaggaac tgtcggtatt
     7801 gatcgtgggg ctgggaaacc gggaagtgac ggcagacgct ctgggaccgc aggtggtgga
     7861 caatctgctg atcaccagac acgtggtcag agcctacggc aaagcagcct acaactgtac
     7921 ccgtatgaat ctggtcagca gtattgagcc gggggtaatg gcgaagaccg gtatggagac
     7981 ggcagcaatc ataagcggtg tggtgaagga gacgcaaccg catgtgctga tcgtggtgga
     8041 tgcgctggcg gcccgcagca ccagaagatt gaaccgtacg atccagatca ccaataccgg
     8101 cattcagccc ggctccggcg tgggcaatca ccgcaatgcc ctgaccgaag agaccctggg
     8161 cgtaccggta attgccatcg ggatccccac ggtggtggat gccgccacga ttgtgggaga
     8221 tgcactggag aagctgatga gcggggaaaa ggagttcgat gcggtcaaat atatgggaca
     8281 gcaccgcatg gcttttgcag aactgaacaa tatgtatatg accggaaagg atatcgattc
     8341 tgtgatcaaa cgggtcagct acacggtctc cgagggaatc aatattgcca tggaaaaaaa
     8401 ctgggcgtaa ctgcatatat atagggtaac agttcatgag ttgagcaccg gattacgagc
     8461 aaaggtagtc acgaagtgac gtaagagcag gtcaaggcga ctttgcgact gacgcgggct
     8521 gaactgtcgt aacaaaaaaa gtgcggaagg agagcgcgaa ccgtgaagaa tccgaaaaat
     8581 actgtctggc ggatcgcact gtttttgctg acagttgtct gtgggctttt gatggcggat
     8641 cggggagaaa atgagtttcc ggtatttgcc tgttccgaag ctttgtcagg agaaagcggc
     8701 agtaccggat tactgctgca gcagaatatg tgggaagtag taccg
//
LOCUS       DAWDHD010000104         8592 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_483_length_8592_cov_10.487291, whole genome shotgun sequence.
ACCESSION   DAWDHD010000104 DAWDHD010000000
VERSION     DAWDHD010000104.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 8592)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8592)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8592
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_483_length_8592_cov_10.487291"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    7314..7413
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2194 a         1734 c         2469 g         2095 t
ORIGIN      
        1 agatacagaa gacaacccgg agaaataata gaattgtgac agcagaaacg cgggaaggag
       61 gaaccatgac gaagatgaca acaaaagtga aaacgcagaa atcagaaaaa gcctggttct
      121 ttctgggagc gttggcagta ttttttatgg gagttccgta tcttgtatta gggaaggact
      181 ctgtgttcgt gtatcacgat cagctggacg gtgaggtgat cgcctatctt ctgcaggcaa
      241 gacatctgtg ggatggcagc ggtgtattgc cggaattcat gaacggtgca gcaaaaacag
      301 cgcttaccat gccggccccc gcctgtgtgc tgttataccg gtggctgcct gcggaactgg
      361 cactggcctg tatgcaggtg gctggcagct ttgccggata tctgggaatg ttctgcctgc
      421 ttggatgggc cggagaaaca tcggaaacac tgcggagaag aaaaggactt gtcggtttca
      481 tagccatgct gaccggatgt atgtatgcgt atctgccgtt tttgccggtg tacgggctgt
      541 cccagtatgg attgccgctg ttaatgtatt gtgtactgcg actgggggaa aaggacagac
      601 cgaagaattt ccggattctc tgttattttt atgtactgct tttcggatgt aattcttccc
      661 tggtgttatc cggttttgct gtgctgggaa tctgggcggt atgggagatc gtcacactgg
      721 tggacaaaag gaaacagttc tccgccggac aggctgcagc ctgggggatt ttgctgctta
      781 cgtatatcgt tgagaacggt tcgttattgc ttcagctgtc cgggggacag ggagaagaga
      841 tttctcacaa atcggaatat ctgctcagcc cggtggactt tttcagtcag ttgaaaacaa
      901 acctcctgca gggaggacag cacagtgtgg attatcatgg actgatcctc gtggtgctgt
      961 tgatgacaac agttgtttta tttttcctaa atcgggcaac gaagaaggac atagcggata
     1021 aaaagaatgt acctgagggc ggagaaaaac ggttatggaa agccgtgggc ctgtcactgg
     1081 ccgttattgc aggctttgcg gcagtggccg cactgtggga cagcagtatc ggaatcgcta
     1141 tccgttccag tctgggtgct ctgaagggct ttcaggccaa cagagtcctg tggctgagtc
     1201 cctgcctgtg gtattttatt ctggggtgca gcctgttatt gctgacggaa cagctcccgg
     1261 aaagagacac aggagcggag aaaacaggga acggacgcag gaacggtgtg atcccgggga
     1321 tcatcgtaat ggccgcaatg cttcttaccg tggcaacagc cgggaagatt ttgctggaga
     1381 gcaatctgaa gcccaatctg caaaaactgg tgaaccggaa ttatgcagcc atgagcttcc
     1441 gggattatta tgccgtggat gtgctggatc aggtacagga gtacctgcgg gagaataccg
     1501 gggaagaacc acaggattac cgggtcgtaa gtctggggat cgatcccgct gcggcattgt
     1561 atcatggatt ttactgtctg gacggctatt caaataacta ttctctggag tacaagcacc
     1621 gtttccggga gatcattgca ccggagctgg acaagagtga atatctggaa gacagctttg
     1681 accattgggg gaatcgttgc tatttgttca gcgcggaatg ccccggctat tatacgatcg
     1741 aaaaaggcgg cttttatttt caggactata cgatcgatgc ggaaagtctg aggcagctgg
     1801 gcggaagcta tctgttgtca gcagcctata tcgatcacag tgaggatacc ggtctggaac
     1861 tgatgaggcc ggaggccttt gagacagaga acagctatta ccgcatttat ctctatcggg
     1921 taatggataa tgaataataa cggagatggg aagatcctat gattggacag gaaaaacggg
     1981 ataaatggaa aaaaatcctg ccggaactgc tggcagttat attgtgcctg gtattgatgg
     2041 gtgcgggagt cagccggaag gaaggctatc acatggacga acttctgagc tttgaactgg
     2101 caaatgccag gtataatccc tggatcgtac ctacccagcc ggaagggcgt ctggccaaat
     2161 tcgtgaacga ggagatcgag ggggacagtg cgggagaggt tctggagaat ctgaaaaata
     2221 cagtaacaga tgtgcttagg aaccggggaa acagcaagct gttgtcttat aaagcagatg
     2281 tctatgagga accggtctgg atcacggacc ggcagtttca ggattatgtt acggtggatc
     2341 agaaagatgc ctttgattac ctgtcggttt attttaatgt aaaagatgac aatcatccgc
     2401 cggtacattt tatgctgctg cataccatga gttctgtctt tcggggaaca ttgtcaccgt
     2461 ggcttggatg cagcatcaat ctggtctgtc tggaaattac attgtggctt ttactgcggc
     2521 tggggagaca gctggcagat gttttttccc tggaaggaaa gggaagacag ctggggatcc
     2581 tggcggtgct tctctatgga atgagtaccg gagcactggg tacggtgctg ctgatccgta
     2641 tgtatgggct gctttcctgt ctgtgtgtcg ccctgttttc cgtccatgtg gaaaaatgga
     2701 agaataacgg gtttgacaag aaaaacaagg gccttatcgc tattacggtg ctgggtttcc
     2761 tgacacagta ttttttcctg ttttactgcc ttctgctggc ggcggttacc gcagtgatcc
     2821 tgctgtaccg gcacaggaac aaagaattat ggtgctatat ccggtccatg gtgatcgcag
     2881 cagtgatcgg actggtattg ttcccttttg caatttcgga tgtgttctcc agcggaagag
     2941 ggacggaagc tctcggcaat ctggcctccg gatttgccgg ttacggacag agactggctg
     3001 catttggcag aattctggcg gatcggacag tgggagcgcc tcttctgacc ggcggatgtg
     3061 ccgccggcgc aatcctgctt ctgggaaagc tgtatctgag cttccgggcc gggaaacagg
     3121 aacagaaagg tgaaaattcc ggaagagatg cggcctggat gctgatggtg ccggtggccg
     3181 gatattttct gctggcatcc cgcatgtcac cgtatctggt ggaccgctat attatgcctt
     3241 tgtttccgtt tgtggcattg ctgcttgctt tgctattgtg tctccttgga aaaaaactga
     3301 cagagatctc cggatggaaa gaacagatcg tgggaggcat gctggtgctg ttgctcgtgg
     3361 tggtacaggg gctgcggctt gcggcctatg atggggaata cctctatcag ggatatgcca
     3421 gacaggaagc attggcggaa gaatatgagg agttcccatg catctgcgtt tatgcgggag
     3481 tgggctatta tgagaacctg ccggaattta tgcattatac caaaactttg ctggtaacgg
     3541 cagaggaact gaaggaacgt aaggatacgg aatccgtgga ggcactggac cgggtgatcg
     3601 tattgatcaa accgggtgtg gataaagaag caacagtgtc tgtgctccgg gataagtatg
     3661 gttatacgtc ggaggaactt ctgctttcgg aaggcgtgca cggagatatg atatatatgt
     3721 tttccagata aggaatgaaa tgataagaaa acagaaatga ggttgaaaca tgatcgcagt
     3781 tcgtttgctg tggcttgccg ttctgctggt agcgataccg gcatgggtgg gaaattgttt
     3841 tttaaatgtg gataaaccct gtagaaatcc ggtatttctg tggattagtg gtcagatgct
     3901 gctatgggcg ggatttcagg tgatctgtgt gccggtgatc ctgttggaag gccgtatgtg
     3961 gatggtgacc gtagcttatt gtggctacat cctggtgctg ctggcagcgg caacggtccg
     4021 gtatttccgg cagtctcgta ctctgcgggc agtccgggaa gcaaagccgg aaaaagcaga
     4081 gattattttg tgggtgctgg ctggtgtgct tttgctggtg cagctggtat tggcagtgct
     4141 gatggtctac ggtgatggtg acgatgccta ttatgtggca atctccgcag cggcagagga
     4201 atccggcagg atgtatcaga agatccctta tacgggaatg catacggaac tggatgtgag
     4261 acacgggctg gcaccttttc ccatctggat cgcatggctg gcccagatga ccggcatcac
     4321 tacggtgatc gtggcgaaga ctctggtgcc ggttgctctg atctccatga cttacggagt
     4381 attctatctt ctgggaagca gggtattatt cgcaggagag ggcgcatacc ggaagaaatg
     4441 ggcgttgcct gcatttttac tctgtacgga agtgctggtg cttttcggag attattctta
     4501 ttatactgta gagaatttta tgattgccag gagcagacag gggaaggctg cattgggaag
     4561 tatcctgatc cccatgatat ttttcctgct gctgacactg cttcgaaaaa tacaggaaga
     4621 acagaagatc actgtcagtt tctgggtgct gttgggatct gtgatgacgg cctgctgtct
     4681 ggccagtacc atgggagcat tgctggcatg catgctggtg ggaacggccg ggctctgtgg
     4741 tgcggtcagt tacagaaaat ggaaactcat actgccattg atcggctgtt gtattccctg
     4801 cattgtgtat gccggaatgt atttgctgtt gggatgatag gaccggagga gaagaatgga
     4861 tgctttaaag ctgtttcagg aatatatggg gaccggactg atcgttctgt ggttcctggt
     4921 gagtctgctg tatctgtggc tcacagagaa aagaaaatat atccgggtga tgtttcttta
     4981 tgtgccgtta gtattgctgc ttgtcttttt taatccgctg gtggcgaaga tcgtctctca
     5041 gatggcggat ggggaaatct attaccggat cctgtggttg ttgcctgtga cgccggtgat
     5101 tgctttcggt acggtgcagc tttgtggaaa actcgccggt agaaaaagat atgtggggat
     5161 aacgttggct attgtgctgt ttacgatatc cggcagtctt atctacagga atcccaattt
     5221 tcagaaggcg gaaaatgcgt atcatgtacc acagagcgtt gtggatatct gtgataccat
     5281 tgaggttccg ggcagagaag tcatggcggc attcccggga gaactgctgc agtatgtgag
     5341 acagtattcc aatgtgattt gtatgcccta cggccgggac attatggtga gcaagtggac
     5401 ggtgcagaat gatctttatg atgttatgga gcaggaggtc attgatgcac aggagctggc
     5461 agaactggcc agagaagagg cctgtgccta tgtgatcctg tcggaagata aaaaactggt
     5521 ggggaagatg caggatcagg aatttgaaga attcggaaga atgcacggat atgtgatcta
     5581 caaagattcc acagtggatc tgtattactt tatgaaggaa tgagatttca gggaaaaaca
     5641 agtggctccc ggagcagcaa agtaactggc tgcttcggga gccttttttg accggaatta
     5701 tttttcctgg gatgcataat ccttggaata gtaattcagg aagtattcaa aacgtttggc
     5761 gatgagctta cgtcctttta cattcaggtg cagattgtcg acaagatatt catcggcatt
     5821 gtcttcggta atgctgccgt acaggttatc catgaaggat acgctgtgga tattggcgga
     5881 gtaacattcc ttgatcacat aagtggataa gacatcacgg ccgttgtaaa tatacatatc
     5941 ggagcttaca tagtcgccgt tctcatccac agcatatgca taggtcggag acataacgat
     6001 aatgcggatc tggggataat agttctgcaa tacttcaatg cttgcctcca gattgccggt
     6061 aaactgcgtg ggatccgtgg gattctcatc actgtacatg gcattgccgg acagataatc
     6121 ggtagcatca tacataatgg caacggtgtc gatcgtgttg aaatccagag ttttcagtgt
     6181 gttgattact tcctctgctt cggccggtgt cttgtcgccc agagcattgg ctgcatcggt
     6241 gtagtagcca tccagaggaa ccccaacggc caggttcacc agccagtaga gacagtaagc
     6301 atccatgggg gcaacggtgg ggtcgtagtt taactgctgt gcagcaaggt aacttccgct
     6361 gatggaacag ttataaacgg tggtatcggt ctctttggcg atcagattgg ccagattgtc
     6421 ggaagagtcc ctgtcatctg cgaagggagc atttccgaag acaacgatat tggaagcatc
     6481 attaatgatc atctgaccgt tttcgtcggt caggggaagg atggaatccc aactgtcgtc
     6541 gtaggaaccc tgcccgtcct tgaagatgtc ggactggctg atcctctgtc cccagtgtgt
     6601 gacgcggtat cctaccacgc cgacaacaat aagaagcacg agagcaaaca gtacgtgaaa
     6661 attcagaaag cgccggatac cggcaggggc cgcagactct tcgccctcag ggatttcttc
     6721 atcggcatct acgtaaaggt cttctatgtc gtcatcggaa tcggtatgga aattgtgcac
     6781 agaaccggaa cgagtattcc cggaatggaa attaccagaa tctgtgtctt gcattgtgtc
     6841 gttttccaga tctacaatct caatatccgg catgtcattc acagaagatg cgtggatatt
     6901 atggttgtta ttgtttcgtt tcatgtattc tctccgtttc aggtaattca ataatgtagt
     6961 ggtaaaaaga gttatattga ttttactatt atacgaaaag aaagtccata atattagaaa
     7021 gatttaagaa attctgaaga aatgggtgaa tgccttgatg tggggaaaat acaatgctat
     7081 aatgttacgg aattgttaca ccgagaatta tgagcagaag ggatagatca gatgaaaaaa
     7141 ttatttaacc atacaaaacg tccggaccgt aactgggaag aagacgtaca ggatggttac
     7201 gattgggacg aggaagaata taccggagaa gacgacggcg aggaatacta tgccgacgaa
     7261 gaagagtatc cggaagagac tgacggcgaa gagtactatg ccgatgagga agannnnnnn
     7321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnacaccgg agaagaagac ggtgaggagt
     7441 actatgccga tgatgtggag tacatcgaag agagtgacga tgaagagtat tatgccgacg
     7501 aggattacga ggaagagccg gctcccacgg gcaggagaaa gaccgggggc aaaaagcagg
     7561 gcggaatcgc tgtaatctgg acaaaattcc tgaacctgag taccatggac cggatcatgg
     7621 cagctaccgg agtggcggta ctgattctgg ctatcgtgac cggaagtgtc tatgccagtg
     7681 cccgggcagt ggatggacag gtatcggagt tcgtcagcgt gggaacacag ctggacggca
     7741 tccagatgat cggtgagcag ggacttctgg cagtggcaga cgcacagatg gcaaagatcg
     7801 cggcagccaa tgcggtagaa gaagatccgg aagaacagaa ggaatatgaa gaggcagagt
     7861 acaccaacgg cggtacggta gaactggagt tggtctctgt acagaaggat ctgaagatca
     7921 aatttaacaa taaaaagacc ggcaagctga tctccaacgt acccttcagc gtaaccatca
     7981 cagatcccaa cggcaagacc gagacctgga ctgatgacga tatggacggt attatctata
     8041 aaaagggtat tacgccgggg aattactccg tagcggtaaa cgcactgacg gatgataaat
     8101 acaaggacta tgttctgccg acagcagcac agaaagtaga agtaaagaaa gatatcgcct
     8161 ataaaaaggt cgatgtctcc aatgagatca agaaagaatc tgagatcaat gctgcaaaag
     8221 aggatacaca gatcaaggat actactgtag agtccacact gcaggatact gtgcagtggg
     8281 tggcaagtac cgcaactgcc aatacttata aagaagtatc caaggatacc attaccgatc
     8341 cggagaccgt aacggtggct tacagcggta agttcctgcg gatggcagaa gtgtctccta
     8401 cagcagaacc aacagcggaa ccaacagcgg aaccgacagc agaacctaca acagagccca
     8461 cagcagaacc tacaaaagaa ccgacagaat ccccgactgc agaccacaca gcgactccga
     8521 cagtaacccc gacggccaca ccaacccccg cggcaacagc aaccccgacc gcggccccta
     8581 cgccagcccc ga
//
LOCUS       DAWDHD010000105         8465 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_492_length_8465_cov_8.987515, whole genome shotgun sequence.
ACCESSION   DAWDHD010000105 DAWDHD010000000
VERSION     DAWDHD010000105.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 8465)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8465)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8465
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_492_length_8465_cov_8.987515"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2569 a         1642 c         2264 g         1990 t
ORIGIN      
        1 tgtaagttaa ggtataagag atagagtttc agtgttcggt tttgaaggaa taaattaaaa
       61 acgatagctt tctgctatcg tttttttatg cattaatttc agataattgt ttcttttttt
      121 aaaatatatg ttataattaa ttgagaaagt agtaaaaggc tgcattgcag gcttcttaaa
      181 atatgtggca taaagaatat ggcataaaga acaaaagcag cgggaagggc acaggagtaa
      241 gtatggatac taagatatta cttgaaaacg gaacaaatga attggagatt ctggagttta
      301 ccctggcagg taattcctac ggaattaatg ttgccaagat caaagagatc atcacctatc
      361 agccagtaac accggttccg aattcacatc ccagtattga aggtattttc atgccgagag
      421 atactatgat caccgccatt gatctgaaga attgtctggg gcgtggtgaa tctgagaaaa
      481 aaggattatt tatcgttacc aactttaata agctggatat tgcattccat gtagaatctg
      541 tattaggtat tcacagagta agctggagag atattattaa gccggatatc accatttctg
      601 cagcagatga gagtgttgct accggcatta tcaagaaaaa tgacaagctg atcattattc
      661 tggatttcga gaagatcgta tctgacatca atccggagac aggactgaag atgtccgagc
      721 tgaatgaact gggagagcgt acaagaagct ctgttcctat tctgattgca gaggattccc
      781 ccctgcttaa caagctgatt gtagattccc tgaaggcagc cggctatgta aatctgatcc
      841 atacagagaa cggtcagaaa gcatatgatg ttattacgca gtgcaaagag gatggtacac
      901 tggatcagca tgttcgttgt atcatcaccg atattgagat gccggaaatg gatggtcaca
      961 gactgaccaa gcttgtgaag agcgatgatg cgaccaagga cattcctatt atcattttct
     1021 catcactggt taatgatgac atgaagagaa agggagaggc tttgggagca gatgcacagc
     1081 tttccaaacc cgagatcggt aatctggtaa gaattgtgga cgaactggta gcagagaagc
     1141 atttagacag atagtaagtt tagagaagca aaaaggctgg gatagtccca gccttttgct
     1201 ataatatcag gatacagtta tggataaaca aacagaagaa attcttcaga aaattgaaga
     1261 agcggaatat gtgctggtgg gcctgggaca ggagtttgac atggaatttt tcctgcaaag
     1321 cagagaagat taccgcagaa tccgggaaca gttacaacag cagaatgtac tttggctttt
     1381 gccttatgtg gaagaacaat tccgcagaca gtatttgccg gagatggaag cagagatcga
     1441 gaagggattg cagaagctgg caaaagtatt ggaaaaaaag agttattttg tggtttcatc
     1501 ttcgctgaat cataaattgg cagaggttcc ctggaaaaaa atgttattga aaaaagagcg
     1561 attcgtggca ccctgtggag actggacgaa aaaacagtgc ccggacgggt gtgaggaagg
     1621 catccagaca gtgacagaag cagatgcgga acaattgcag gagagtttta agaagctgca
     1681 gactaacggg ttttcggttc cggatttggg aaaatgtcca aagtgcggga aaaagttggt
     1741 actcaacaat gtctatgccg gccgatacga tgaaaaagga tatttgaaaa cctggacaga
     1801 atatcagaac tggctgcaaa ataccctgaa tcataaaatg gtgttgttgg agattgggga
     1861 ggggaaccgt tttcctacga ttatacgttc cccttttgag agaatcgctt tgtttcaaca
     1921 aaaagcagac ctttattgta tagatggaga acaaaatccc attgcctggc tgctcagcct
     1981 gtgttaaact taacatgcta gtaacttgtg agaaagcgga ggaatgacat atgccttcac
     2041 aggaggaata cctggataat ttattaaaag gaattgaaac agaaaatatg gaaacagagg
     2101 ttacaaagga gccgaaaatg accgggacgg aggaagaaac tgccaaagca ccggaaacgc
     2161 cggagcctgt ggaagagagt ggaactcagg tcgatatgag cgatatggat gatctgctgc
     2221 agtcggcgct ggatgctcaa aaagctgatt catcagagca ggaaacggaa gcaagtcagc
     2281 ctgtagcgga aaatacagcc attcttccag aggagacagc gtctatgtct gaggatgaaa
     2341 ttaacaggct attgcagcag agccaggagc aatccgggga tgctccggca caggagagca
     2401 acgatgctaa tgacgatctg atcaaaatgt tggagaatgc ggatgacgag ggattgtcaa
     2461 atattctgga tcaggtgaca ccggagactg cacagccgga ggaaacagca gagcgggaag
     2521 aagacaacgg caagcgcgga aaaaagaaaa aagaaaagaa aaagaaggaa aagaagccgg
     2581 gattttttga acggtttaag aagaaatcca aggaagaacc ggaatcagca ccggatgaga
     2641 cagaatcagc acagcctgcg ggagaggaag ttacatcgga tatcccggaa actgtgccgg
     2701 atgatcttgc ggatctgtct tccctgacgg atggcatgga tgatacagac aaaacaccga
     2761 tggaggcttc tgacaacacg gaaagtgtag aaagtacgga aaatgctgcg gaaaacggag
     2821 atgccggcgg cctggatgac gatctggacg ctttacttgc gggagcattt ccggagcagt
     2881 cggagaacag cacggataat gcatccggag aaccggaatc tgcagatgtg atggatattc
     2941 tgaaggctgc aggcgcagac atcatggatg agccggaaca gaagaaagag aaaaaaggct
     3001 tttttgccaa attattagat ctgttcaccg aggaggacga ggaagaagag gaagcggatc
     3061 agttgcagct gtccgatgag aacaagaaga tcctggaaga gatggacaag ggcggcaaga
     3121 acatgaaggg aaaaaaacag aaaaagccta aactagataa gcacgccaag cccaagaaac
     3181 cggcaaacga aaaaaagaaa atagataagc cggaaaagga agataagccg tcagtatcgg
     3241 agaaaaagct ttctccgaag aaaatcctcc cgattgtagt ggtatgtatt tctctggggg
     3301 cggtgattct gttgctgggt aacttcctgg cggattatac cgcgaagcgc agcggtagag
     3361 aagcttacta tgccggagat tatcagacct gttaccagaa tctgatgggc aaggaactgg
     3421 atgagacaga acaggtgatg tacagtcagt ccgagagtat tctgaccatc cgtatgtggc
     3481 ttcgggagta cgaagtattc gtaaacgaag gctctgaatt ggaggcattg gacagcctgc
     3541 ttcaggcagt cagggattat ccgacattgt taaattatgc gacacagtgg aatgcgcagg
     3601 atgaggtaag tctggcattc caggatattt tgaatatcct ttcccagaaa tatcagttaa
     3661 gtcaggaaga ggcacaacaa attgcggata tttccgataa tgtagaatat acgaagaatg
     3721 taatgttggt tctgcaaagg ctgggactgg gattctggga gttcccgcag ggaacggtaa
     3781 cggaggaaac atctgcacct aatacggagg aggcgcccgc acagctgccg gatcctctcc
     3841 ccgaggaaaa ggagattcag caatgatcac agtgctcgac tatgatgctg gcaacataaa
     3901 gagtgtagaa aaggcattga attttctggg agaagaggta aagattacca gagacagaga
     3961 ggaaatcctg tccgcagacg gtgtgatcct gccgggtgtg ggagcgttcg gtgatgccat
     4021 ggaaaaactg caccagtacg gactggtgga tgtcattcat gaagtagtgg acaggcagat
     4081 cccttttctg ggaatctgtc tgggcttaca gctgatgttt gagagtagtg aggagacacc
     4141 gggagtagag ggactgcacc tgttagacgg taagatccga aggattcctg cggcagaaga
     4201 actgaagatt ccgcatattg gctggaatga ccttactttt cccaatgaag gaagactttt
     4261 ccgaggcgtg gaagagcatt cctatgtata ttttgtacat tcctattatc tggaggcagc
     4321 agatccttcc attgttactg ccaccacaga atatggaact ttgattcatg cttccgtgga
     4381 aaagggtaat atttttgcct gtcagttcca tcccgagaaa agttcccggg tgggaatgaa
     4441 aattctgcag aatttcatag agatcacaca agagttccgg gaaaaagata agcagggagg
     4501 aagatgatat gcataccaaa agagtaattc cctgtctgga tgtgaaggat ggcagagtag
     4561 taaaaggtgt gaattttgtg aatttccggg atgccggaga tccgtcggag gtagcatctg
     4621 cttatgatgc agccggtgcg gacgaagtgg tatttttgga cattacggct tctgcagaca
     4681 gtagagcaac gcagttagag tgggtgcgga aggtagccag caaggtattt attcctttca
     4741 ctgtaggtgg aggaatccgt accgtggatg attttaaggc gatattgaga gaaggagcag
     4801 acaagatttc tgtgaattcc gcagccatta tgaatccgca gctgatcagt gatgcagcag
     4861 agaaatttgg cagtcagtgt gtggtggtcg ctatcgatgc caagaagcgg gcagacggcg
     4921 gtggttggaa tatttataaa aacggtggtc gtgtggatat gggcatggac gctgtggagt
     4981 gggccagaac cgcagagaag atgggagccg gagagatcct gctgaccagc atggacggtg
     5041 acggcacgaa ggaaggctat gatctggagc tgaccagggc tgtggcagag agtgtaagca
     5101 ttccggtcat tgcatccggc ggtgctggac agctgtctca tttctatgat gctctgacag
     5161 agggaaaggc agaggcagtt ctggcggcat ctctgttcca ttacaaagaa ttagagatca
     5221 ggcaggtgaa agaatacctg cgggaaaaag gaatcagtgt ccgtctctag ggacgacagg
     5281 gaggatttga ctatggcaat gatcacgaat gactggctga cggctttggg cggagaattc
     5341 cacaagcctt attacagaca gctttttgaa tttgtcaaaa atgaatacaa caccaccgtg
     5401 gtcttcccac cggcagatga tattttcaat gcatttcatc tgacgccgtt aagcaaggta
     5461 aaagtggtga ttttaggaca ggatccttat cataatgtgg gacaggctca tggactgtgc
     5521 ttctcggtga aaccggatgt agagattccc ccttctctgg tgaatattta tcaggaactt
     5581 cacgatgatc tgggatgcta tatccctaac aatggttatc ttgtaaaatg ggccgagcag
     5641 ggtgtcctga tgctcaatac ggtgcttacc gtgcgggcac acgtggccaa ttcccatcac
     5701 gggaaaggtt gggaagagtt tacagatgcc gccatccggg cactgaatgc acaggatcgt
     5761 cccattgtgt ttatcctgtg gggcagacct gcccagatga agaaagctat gttgaacaat
     5821 cccaatcatc tgatcttaga ggcccctcat cccagccccc tgtctgccta ccgcggtttc
     5881 ttcggcagca gacctttcag caagaccaac aacttcttga aagaacacgg cgtggagccc
     5941 atcgactggc agattgaaaa tgtgtaaatt gtgagttacg agccatgcaa aaatagagtg
     6001 gaacacctcg atgggcgctt gcgcacaagc gaggctggtt cactctattt ttcatggctc
     6061 gtaacgtagt gctgccatac cctccacgaa cgtccgcctg tcgcgaacgt cctgttcgca
     6121 ccaccctgat tttcaatact tttcgagaaa atgtcatcac cgtccgacaa acatccccgg
     6181 aaggatgttc catccgctgc ggacgttctg cttttttcat aatatctgta caccataggc
     6241 atttctgttg cagccatttc tattgctttg gtgcaaatcg cattaaaata gcaaaatcag
     6301 tacaaaaaaa cctctaaatc aaacattttt tgtactgatt ttattatttt tcccatttta
     6361 gcgaaaataa gctcttataa aaggccaaaa tctcctgttt ttgtattttt ttagcaaaaa
     6421 aggacgtttt taatacaaac agaactcccg gaggaatggc atcactttct atttctttac
     6481 tgacatcttt tctcaataag tatttcccct attgacaaaa caaggaatcc ttactatagt
     6541 gttcctgtaa agaaccacta aaaggagtgc ctatggaaga agcggcaatc atagaacaac
     6601 ttatcctgtt cggactgagc aggcaggagg ctgccattta cctgtgtctt cttcaaaatg
     6661 aagaactgac aggctatgag gtggcgaagc tgacggggat ttccagatcc aatgtctata
     6721 atggactggc atcgttggtg gagcatggag ctgcttatgt gatcgaagga acctcctcaa
     6781 aatatctggc tgtagcatta tccgagttct gcgataaccg gatccggtat ctgcggaaag
     6841 caaaagaacg tctggtggcg gatggcccca ggaaaaattt acctagagaa ggctatatca
     6901 ccatcgaagg ctatgaccac atctgtgaca agatccggca catgcttctg ggtgccgaga
     6961 agcgtattta tttttccgca acaggtgaat tcctggagca atggtcggag gagatcaggg
     7021 agctggtccg ggcacagaaa aaagtggtgc tgatttcaga agacaacaga gaaccttttc
     7081 cggaagatgc ggaattaaag gccgggatca tagaatacct ggtgccggag catttcagag
     7141 agcctaagga agaggaacag cagatggatc agatccgtct catcatagat tccgagtaca
     7201 ttctcaccgg tacggtgacg ggaaagagca gtgatacctg cctttacagt ggccagaaga
     7261 attttgtcag agtcttcaag gatgccatgc gcaacgagat cgccctcatt cggttgaagc
     7321 agggtgagca ataattatgt agaaaacgct tttgccgggt ggaaacggcc gcagcgttct
     7381 cataaatagg ataaaataga aagggataga aaaatgagta tcagagtcgc aattttaggt
     7441 tatggtaact taggaaaagg aatggaatgt gcagtaaagc agaatgatga catggagctg
     7501 gtagccgtat ttaccagaag agatcctgct acagtaaaga ctttaacaga tgtgaaggtt
     7561 tataatgtag atcagttact gtccatgcag gacaagatcg atgtcctggt gctgtgtgga
     7621 ggctccgcaa ccgatctgcc caaacagacc gcagagtatg caaaatattt caacgtggta
     7681 gacagctttg atacccatgc gaagatcccc gagcatttcg cacaggtaga tgctgcagca
     7741 aaagagggcg gcaaggtagc catgatttcc gtaggctggg atcccggtat gttctccctg
     7801 aacagactgt atgccaatgc cattttaaga gacggcagcg attatacctt ctggggtaag
     7861 ggtgtcagcc agggacattc cgatgccatc cgtcgtatcc ccggtgtcaa ggatgccaag
     7921 cagtatacca ttcccgtaga gtccgcactg gaagctgtca gaaactgtga gaatccggtt
     7981 ctgaccacca gacagaagca taccagagag tgttttgtgg tagcggaaga gggcgcagac
     8041 aaggcacaga tcgaagagca gatcaagacc atgcccaact attttgcaga ttatgatact
     8101 actgtacatt tcatcactga ggaagaactg cagagagatc acgcaggtat ccctcacggc
     8161 ggatttgtac tgcgtaccgg caagaccgga ttaaacggtg agcacaacca tgtgatcgag
     8221 tacagcttaa agctggattc caacccggag ttcacttcct gcgtactgac cgcttatgca
     8281 agagctgctt accgcatgaa tcaggaaggc cagaagggct gcaagaccgt atttgatgtg
     8341 gcacctgctt atctgagcac tctgactccg gaagagatga gaagcgcgtt actgtaaata
     8401 agatagatgc agaggagata cttatggaaa aaaaggcatt tgttaccaaa gaaatgatcg
     8461 aaaag
//
LOCUS       DAWDHD010000106         8266 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_509_length_8266_cov_10.270613, whole genome shotgun sequence.
ACCESSION   DAWDHD010000106 DAWDHD010000000
VERSION     DAWDHD010000106.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 8266)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8266)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8266
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_509_length_8266_cov_10.270613"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1953 a         2153 c         1640 g         2520 t
ORIGIN      
        1 tcttacggga tcccaaaagg gcgtccgtat tcctggtatc gttcaggtca taggtcggat
       61 aacttgccat ggccagaata ttaccggtat ttacctccat aataatgcag cccacatttt
      121 ccgcaccgtt acctggatgt accgcatcct tatgctcatc gttgaattgt ttcagatatt
      181 tttccacgat accctggata ttggcatcaa tggtactgtg tatcgtattg ccgtccacgg
      241 ccggtttgat ggtccgctcc aggttggcat catcattcag atatccatac tctcttccgg
      301 tggttccgtt cagcacatca ttgtaatact cttccagtcc gtactgtcct tcgttgtccg
      361 atctggagaa tccgatcacg tccgccgcca gggaaccgtt aggatagact ctcttgtatt
      421 cttcctggaa ccagacgccc ttgatcttgc tgtcctccag tgctgccgcc tgaaaaccgc
      481 tgatctgatc ataggtcagc cttctgagag ggacatacca ggaagaatcc ggatgactga
      541 tcacatactg tctcactgcc gtcatatcca gatccgggaa atttgcggac agcgcctcca
      601 tcgtcggttc cagataagtc tcacggctta acattacata ggaatcaatg accagattat
      661 ataccttctc gctgactgcc agtttcgttc ctttggcatc cacgatgtct ccgcggcgga
      721 acggcaatgt cacggagtcg tatttctgct gtgacaaaac ctgtttttcg taggttgctc
      781 catccgcttt cacaatccag gacagacgga cagacagcag aataaatgcc agtatgatca
      841 gggcatacag gcataaaagt tttttctgca tctttatgga aaatttctta ggcttctgtt
      901 ccttcacgct ttctgcctcc cagacttagt tctggctggt atccgcctga cgcatatagt
      961 cgttgctgct gcttgcgtag gtaatcacct gtccctcggt ggcataggtc attcccagct
     1021 cgccgatggc aattctcttg atctcttcca ggttaatatt actggtgatc cggctgtact
     1081 cttcatcatt tgacagcttc agagagttta gctcactctc cattctggac acctctgtga
     1141 tcttggtggt cagttctgac tgcatctgga tataatttac cagaaaagca gcgcaggcga
     1201 tcagggctcc catcagaaaa gtgacgtagc cgatgctcat atgtcttgct ttttctctgt
     1261 tttttcttgt ctcatgagac agttgtctcc ggggctcctc catctgcctg cggatctgta
     1321 agtctcttgc tgtattatca tatatgtaag gagtattgct tactcttctt ctctgtgcca
     1381 tactctttct ccccgctttc actactaaag tcttttattt tttctcaaat accctgagct
     1441 ttgcactctt ggatctgctg tttacttcca gttcttccac actgggaaga atgggttttc
     1501 tggtgatcac ctttcccttg gagaccttac cgcatacgca caccggaaat tccggcggac
     1561 aggtgcaggg attctcattc ctgcggaatg cgttcttcac gatccggtcc tccagggaat
     1621 ggaaggtaat aatgcagaac cttccgccgg gattcagcag atcgatcagc tcgtccagcg
     1681 cctttttcag tacttccagt tcctgattac attcgatacg gattgcctgg aaagtctgct
     1741 tcgaaggatg tccgttctgt cgcatctttg ccgggactgc ggctttgatg atctcattta
     1801 attcaaaggt ggtctcaatg ggcttgtctg ccctgttttt cacaatgtgt ttcgctatat
     1861 tcttggcaaa aggatcttca ccgtaatccc ggatgatgtg atacagttcc atctcggaat
     1921 aattgttgac gatatctctc gctgtcaggg tctgcctgcg atccatccgc atgtcaagag
     1981 gggcatcaaa ccgataggaa aatcctcggt cttcggtatc cagctgatag gaagatactc
     2041 ccagatccag cacgatcccg tccacgccgg tgacaccata ttctctgagg gcctccactg
     2101 cattcacata attgttgcgg atcacggtca ctttatctcc aaacggctgc aatcttgcgg
     2161 atgctgccgc gattgctgca tcatcctgat caatgccgta gaaatgtccg tttttcaggc
     2221 ggctgcatac ttcgtaggca tgtcctcctc cacctaaagt tccgtccaca tagatgccgt
     2281 ccgggttaat attcagttgt tcaatggtct catgcaacat cacagaataa tgattgaatt
     2341 ccacgacggt catcctttct cggtcacgtt tttcacccgt taaatggtca gtcccagtcc
     2401 ttccatggca ctggtgatct catccatatc ggcatcggag ttcatgttct cccacttatc
     2461 cttgctccag atctccacac ggctgccgac gccgaccagt accacgtctt tttccagtcc
     2521 agcgaactct ctgagtgctg ccggcaacag gattcttccc tgcttgtcca cttctaccgt
     2581 agctgcaccg gccaggaaga aacgggcaaa ttgtcttgct tctttattga tcagaggtaa
     2641 tgaggtcagc ttctgttcaa aagcattcca gtcatcgttg gcatacacaa ataagcagcc
     2701 atccattccc ttggaaatca caaattcctc tcctaagact tctcggaatt tcgccggaat
     2761 gatcaacctg cctttcgtat ctaacgtatg gttgtactcg ctcataaaca tcgcactcac
     2821 caccattcat ccaaaatggg actgccacga aaaggaggtc atttttctaa aaatctacca
     2881 ctttgtggca cttacctcca ctttctccca cttttattat gattttatac taaaataggg
     2941 tattttacaa gagaaatcaa gtggcttttt tcgacagcac cacgcaaaaa agcgccttgt
     3001 catacgacaa aggcgctttt tactacaata tctggtataa aaataagagc aaacacaata
     3061 tctatgtatt tgctcctgtt tcgatattct taataaattt ttaaaaattt ttaatatttt
     3121 tccactttcg gctgtttttc caggcggata cgtaccacaa cgtccgtcac cactgcgaag
     3181 atcaccatgc acaaggataa tacctgtgat acggcgaggg tcgttccggg gataaacagg
     3241 gtatctgtcc ggataccttc gatccagaat ctgccaagac catagcctcc gaagtacagc
     3301 agacagatct caccctcgaa ctttttgtgc ttgcggtaca ccagcataag gatcaggatc
     3361 atcagattcc acaggctctc gtagaggaaa gtgggatgta cctgaatgta attggtccca
     3421 tcgataatgt gagctgctac attctcagaa atatcccggc tgcgcacagc ttcgatagga
     3481 agccgcatgg ccagaaaact gtccgtatat tccccgaata cttcacggtt cataaagtta
     3541 ccccagcggc cgatgatctg tcccaggatc aaccccggca cacacagatc tcccagccgg
     3601 aacgggttga tcttcttgat cctgcaataa atgagcagtg tcaggaatgc tccgatcaca
     3661 ccgccgtaga tcgccatacc gccgttacgg gtattaaaga tgcttaacag attgtccttg
     3721 taatagtccc aggcaaagac cacgtaatag attctggctc cgatcacgga aaagatcacg
     3781 gcataaatgg cgaaatccca gatcgtatcc ggattttctc ctgtcacctt tgcctgatgc
     3841 accgccatca gaatgcctgc cagtaccccg attccgatga tcaggccgta aaaggcgatt
     3901 tcgaaaccga atacggaaaa gcttctggga acattctcca ggtaaatgcc taaatgggga
     3961 aatgcaatat ctcctgcatt catcataata tttatttcct ttcttggtac tatccggctt
     4021 ctgcgcgctt cgcgctcccg aatccggcac atacatttgc attccgggct atcgccctac
     4081 ttgctcatgt gcatatacta tccggcttct ccgcgctttg cgctctcgaa tccggcacat
     4141 acattcgcat tccgggctat cgccctactt gctcatggct ccatactatc cggcttctcc
     4201 gcgcttcgcg ctcccgaatc cggcacatac attcgccttc cgggctgtcg ccctacttgc
     4261 tcatgtacat atactacccg gcttctgcgc gcttcgcgct cccgaatccg gcacatacat
     4321 tcgcattccg ggctgtcgcc ctacttgctc atgtatgttt gatccgctaa tagacagatt
     4381 actctgcctg caagcagtct cgtaatctgt ctattgcgga tcttagtatt acacgttgaa
     4441 gcggaacagg atcacatcgc cgtcctgcac tacataatcc ttgccttcca tgccgaccag
     4501 acccttttca cgggcagcgg ccagagagcc ctgctccagc agatccttgt agtttactac
     4561 ttctgccttg atgaagcctc tctcgaaatc ggagtggatc ttgccggctg cctgaggtgc
     4621 cttggtgcca atcttgatgg tccatgctct agtctcatcc tctcctgcag tcaggaagct
     4681 catcagacct aagatatgat aacttgcctt aatcagtttc tccagaccgg attcctttaa
     4741 tcccaggtcg tctaaaaaca ttgccttctc atcatcatcc agctcggaga tctcttcctc
     4801 gatctgtgca cagatcacga atacctcact gtgttcaccg gctgcgaact ctcttacttt
     4861 tgccaccatc tcattggatg cgccgtcatc tgccagatca tcctcggcta cattggctgc
     4921 atagatcaca ggcttataag tcaacagatt gtattccttt aacagagcca gttcatcctc
     4981 atcggtaaca tccaggctct ttgccatctt accgctctcc agatgctcct tgatacgctc
     5041 aagcagtgcc agctccttgg cataggtctt gtccattctg gctgccttga ctaccttgga
     5101 cagtcttctc tccagtactt ccagatctgc gaagatcagc tccagattga tggtctcgat
     5161 atcacgcaga gggttcacgc tgccgtccac atggatgaca ttaggatcct cgaaacatct
     5221 taccacatgt acgatagcat ccacttcacg gatattgctt aagaactggt tgcccagtcc
     5281 ctcacccttg gaggcaccct ttaccagacc tgcgatatcc acgaattcga tcactgcggg
     5341 agttaccttc ttggaatgat acatctcgcc cagtgctttc agtctgtcat ccggaactgt
     5401 taccacaccc acgttagggt cgatggtaca gaagggatag ttggcggatt ctgctcccgc
     5461 cttggtcagg gaattgaaaa gtgtgctctt tcccacgttg ggaagaccta cgattcctaa
     5521 tttcatgtta tcttttacct gtccgaaaac cctttattta tgcgggtttt cgcctttctc
     5581 tatattttta ttacaccctt tttacaccct tttttcaact gtgggtgtag ctgcttcaaa
     5641 ttgctgaaca gcttttgcaa gggactcgtc cgtaacatgg acatacctgt ccattgttgt
     5701 tttaatactt gcgtgtccta atagttgctg taaaacttta ggttgtacgc cgcgttcaat
     5761 cgcgcgtgta gcataagtgt gtcgtaaggc gtgcatacaa aaacgcttaa taccagcttc
     5821 atcacacaat ttatataaat gtgtatcgta ggaactgttt ttagcaggtt ctcctgttcg
     5881 ccagttgata aataccaaat cccgcatgac aagacatttc ttttctcccg ttctgctgtc
     5941 tgtgtactcc aaaatctgtg aaagcatttc agactctttc cgggtatctt tttcatcata
     6001 acaacttttt aggattgtat atgcgcgttc tgtaagcgga atagtacgat aacttttttt
     6061 cgttttaggc ggaccggcac gccaatatct ccggctgtgt cgatactcta aactcttatt
     6121 tacagttaaa gtacgttttt tccaatcaat agcgtcccat gtcagaccaa tcaattcagc
     6181 cgttctcaaa cccgtttcta ataacaaagc atactgtcta taattatgag aacgctctgc
     6241 ggcttcaaga aacttttcct gttcctcaac ggttaagtat ttaatatcat ctactgcccg
     6301 gactggtttc gtatatcgaa caccattcat agggtgtttt gcaataatat cattcataac
     6361 tgctgatttg aacatagttc ccattgaaat ataggtctgc cggattgttg agcctgcata
     6421 ttctgtttcc attcgattta agacagcttt acagtgcata ggttttacat cagctataca
     6481 catagttcct atcaatggtt gaatattttt ttcgtaacgc tcacgataat ttcttatcgt
     6541 atttggcgca aggtcacaaa tgatattatc aatccaataa ttgaaccatt tgtctactgt
     6601 catgtcaggc gcagtaacga ttagattatg tgtatcttca tactttgcgt cagcaagcca
     6661 tttttttgct tcgggcagcg ttttgaaata tccattttga cgcgcaccat ttttagcata
     6721 aaatcttgcg aaatacaaac cgtcctttct ttgacaaata ccttttccac actctttgcc
     6781 ttttaaattt tttcccatga cggactcctt tcacgttaaa aaaaagagtc caacgcaaat
     6841 ataaagtata tcacaaggac tccgtttttt caatcttaac agacaaatat ttcagctatt
     6901 aaaggctata taatcaattc tcggcgaatg tattcctcaa attctttacg cttcactaac
     6961 tttcgtgttc caacatataa aacaaaaggg cagtttggct gttttaacat ggagtcaatc
     7021 ttattgatac ctatgttgct atattcagcc gcttccttta ttgttaatgt tattttcaaa
     7081 tgaataggaa cttttacttc tgtttcctta tataaatgca gttctttttc ttcactgcta
     7141 tttgatttat tcattagaat ttccccctat cttttttcaa tttaacaaaa aataggctcg
     7201 tcagacggtc ttagcaaacc tcacaagaat ttcacttccg catggttctc atgcagctct
     7261 acccgttgcc tgcgacgctt ctaacgctcg gactacggcg gtaaaggagt atcattatac
     7321 cgatatatgg attgtggcgg cagagttgct aagtctgctt tggaacactg ataattctcg
     7381 ctcgttttgt ccctcctttc ggtcatggcg tatcagccca acggctcccc atatatcgaa
     7441 tgtatccgat ttgccttata ctgcgtcgcc gttctattgc gccgctttct ttgtcaaaga
     7501 gcagggggct gttctgcgat atggaacgga aaagggggaa gcgttccaat accgcgttgc
     7561 tttattcagc cctaaatgtg aggattgccc tcattagttt tgcttgcagc ctttcacgta
     7621 tttcttcgtc tactccaaaa taaacatttc ctcgctcatc atagagcttc cgcatggaaa
     7681 gacttgctat gtagccgctg aaatgctgca atacaatctt cattgcatcc gggtcgcctt
     7741 tgctcgccgc caaaataacg ggatagggaa caagggcatt ttccgggtag tcgttttgat
     7801 taccattcaa tccattcatc agcgttttcc tccaaatatt tttttaactg ctcaaaggag
     7861 cttgtccggc gatactgtac cgtacttctt gagatattga gctgctttgc gatttctata
     7921 tcagtcaatc cctcaaagta atacagcagt acaattttgc gccgttcttc gggtaaagcc
     7981 cggatagctt caagtagcag ttttggggtt atttcttttc ctgccatttg ataagacggc
     8041 tctgcttctt catcttgaaa atacttatcg acggtataaa gctgccgttc ctcatacaag
     8101 gcaaggtcgg aaaaagtaac ttcccgaact ctgcgccgac gaatttcttc atgggcgttg
     8161 cacgcttcat tgtgcagcac tgatttacag aacttttgaa acgtacattg tttttcaaat
     8221 tccctgcgat taggtgtcat ggtgttcacc tcctttctgc cgaaag
//
LOCUS       DAWDHD010000107         8265 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_510_length_8265_cov_10.499147, whole genome shotgun sequence.
ACCESSION   DAWDHD010000107 DAWDHD010000000
VERSION     DAWDHD010000107.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 8265)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8265)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8265
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_510_length_8265_cov_10.499147"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1809 a         2339 c         1761 g         2356 t
ORIGIN      
        1 atctgtgccg gtttcccagt gccatctccc cgttggttcc aagatcgatg agcagggtgg
       61 aggcctcctg ttcgagcatt ccactggcca atattccggc agtaatatcg ccgcctacaa
      121 aggctgacaa tcccggtatg atatgacagg ccactcctgc aatttctgtc tccactgcgc
      181 cgctatgact gactgtaaaa ggtgctttgc ccagttcctc cggattccag cccatgaaca
      241 gataggtcat ggtggtattt gccgcaacga ccatctgcag agtctcctgc ggctgcagct
      301 ttttggaaaa acgctgcact cctttttcca tgaggtcacg gacttttttc tgcatatctg
      361 ccgcttttcc cggatccttc gctgcctcta tccgtgagag cacatcggca ccatagctga
      421 cctggggatt cacactggga aagctgtcca ccactttacc ggtcatgtca tacaactgca
      481 tggcaatggt agtggtacca atatccacag tcatcagccg tttaccgtcc ggattttgta
      541 atgttaacgg aggaagttcc agcttttccg tcaccacctg catcccccgg atcactcctg
      601 gacatctacc acaaccggta cagatcaggc atctggttcc cttttttcct gtgggattgg
      661 tactgctcgt attatacaaa ctgattcctc tcttcatcgt aatgatagtc aatggctgcc
      721 agtttatctg tgatctcctg ttccttcaca tccagatcat cgcagagctg tttcaggctt
      781 ccgtagaaat cacggagttt taaatttata aagcttaata acattacggg atcctgtggt
      841 atcataacgt tttcctctct tttcctgttt ttgacagagt tatattgtaa acgaaaggag
      901 caaaaatgac aagtatcatc atctttgctc ctgttttatt ttacagatta ttgatttttc
      961 gatccttttt cgcacttggc ggccaattga tcattctcca cggtaatgac aatggtatcg
     1021 ccttcttcta cctgatctgc cagaatcatc ttggctgcca gggtctccac gtacttctgg
     1081 atataacgct tcaaaggccg tgcaccgtag accggatcat aagcattgtc cacgatatac
     1141 tgcatagctt cctccgacag acggatggtc agctgcttat cctccagacg tctgttcaga
     1201 tcggcaatga tcagatgaat gatgccgccg atattgtcct tggtcagagg cttgaacagg
     1261 attgtctcat ccagacggtt cagaaattcc ggacggaaat gagcccgcag gtcgttcatg
     1321 actgccgcct ctgcctccgg cttgatggtg ccgtcttcgt tgataccgtc caacagatag
     1381 cttgcgccga tgttggaggt catgatcagt atggtattct taaagtctac ggtacgaccc
     1441 tgagaatccg tgatacggcc gtcatccagt acctgtaaca gtacattgaa tacatcggga
     1501 tgtgccttct cgatctcatc gaacagtacc acgctgtaag gttttctccg gacagcttcg
     1561 gtcagctgac caccttcctc atatcctaca tatccgggag gcgctccgat cagacgagag
     1621 acagaatact tctccatgta ctcactcata tcgatacgta ccatattatt ctcatcgtcg
     1681 aacagtgctg ccgccagact cttggccagc tccgtcttac cgacaccggt aggtcccagg
     1741 aacaggaagg agccgatggg cttgctggga tccttgatcc cggctttgga acggatgatg
     1801 gcttcggtga ctttggtcac accctcgtcc tgacccacca cacgtttgtg cagttcttcg
     1861 tccagatgga gggtcttgtt acgctcactc tcggtcagtt tggctaccgg aatgccggtc
     1921 cagcgggaaa tgatccttgc gatctcttcc tccgatactt tttcatgtac cagagacaga
     1981 tcggattctc ctaccttatc ctcttcctgt gccagctgct tctccagtgc gggcagttcg
     2041 ctgtatttta atctgccggc cttttcatag tctccttccc gctctgcgat ctggatctca
     2101 ttattcatgc ggtctctctc ttcccgcagt ttggaaagtt tatccacgga tgccttctcg
     2161 ttatcccact gtgccttttt agtgttgaac tgatccttta actctgccag ttccttctgc
     2221 agatctgcca gacgttcctt actgatccgg tcatcctctt ttttcagagc ggtctcctcg
     2281 atctccagct gtgtcacctt acgctgcagt tcatccagct ccgtaggcat actgtccatc
     2341 tcggtcttga tcagggcgca ggcctcatcc accaggtcaa tggccttatc cggcagaaat
     2401 ctgtcggtaa tataacggtt ggaaagcacg gctgcactta ccagtgcgtt atccatgatc
     2461 ttgacaccgt gatatacttc gtaacgatcc ttcagtccac gcaggataga gatcgtatcc
     2521 tccacggtgg gttcttctac cataaccggc tggaaacgac gttccagagc ggcatctttt
     2581 tcaatatact gtctgtattc atccagtgtg gtggcaccga tacagtgcag ttcaccacgg
     2641 gccagcatgg gcttcaacat attgcccgca tccaatgcgc cgtcggtctt gccggcaccg
     2701 acaatggtat gcagttcatc gatgaacaga atgatctgtc cgtcactgtt cttcacatca
     2761 tccagtacgg ccttcagacg ttcctcgaat tcacctctgt atttggcacc ggctaccagt
     2821 gcgcccatat ccagtgcgaa gatcttctta tccttcaatc cctgagggac atcgcccttg
     2881 acgatacgct gtgccagacc ctccaccacg gcggtcttac cgacaccggg ttcaccgatc
     2941 agcacggggt tattctttgt cttacgggac aggatacgta ccacattacg gatctcgctg
     3001 tcacgtccga tgacgggatc cagcttctgg ttccttgctt tctccaccag atcctgccca
     3061 tatttttcca atgtatcata ggttgcttcc gggttgtcac tggtgactct ctggttgcct
     3121 cttacggtag acaatgcctg caggaaacgt tctctggtga tgccgtattc cttccagatc
     3181 tcggcgatct ccttgttggg attcttgatc atggcaagga acagatgctc tacggagaca
     3241 tattcgtcac ccatctgttt tgcttcgtcc gatgcgctga ccagtacctt gttcagatac
     3301 tgaccgacat aaggctgacc accctgcacc tttactctct tctccaaagc ctgttttgcg
     3361 cggtttacaa aatgatcctt ctggatctcc atcttttcca caagctttaa gatcagactg
     3421 tcatccagcg tcagcagact gtacagcaga tgttcctgtt cgatctcctg attgccatat
     3481 tccatggcga gcttttcaca gccattgact gcttctatgg atttctgtgt aaatttatca
     3541 atattcataa tcgttcgcct cctcacataa ggttatgcaa tttgcaacta ttcagagcca
     3601 ttaaatattg cctaagcttt gttgattgct tgcattcata taaagctttg gcaatattta
     3661 tttggcgagg tagcctcaat gagcaaaaag gaagctgtgt aacagcgttt tgcgaatgag
     3721 gctctgaata gttgctttat ttattttctt actcgcgaac agattcacag gctttctgtc
     3781 tcatctgttc tatatttaat ataacacata gtcagagaaa atcaattaag aaattataaa
     3841 ctttatatgg aatctttcga aaattatttt ccattcacgc ggaagattaa tgccgttagg
     3901 agaaaaatca ctctccagat actcattttt cacaatttaa gtatctatgc ctgaaatttt
     3961 tctgtataga cccatacgcc acatatattc ctgaaatatt gcattgaaaa gtacctgcgg
     4021 atcacttttt tctctataga cccaaaatag cgccgtccgg tgtcgtgttt gcaaaaacac
     4081 ggcatcttgg cagcgccatt tctctttttc cgttattgta acctacagta acactttatt
     4141 tttcggtaac gccctttact tcatatcctg cgtcaactac agccttggtc agtacttcgt
     4201 ctgccacatc agaagccagt tccacagtag cattcttctg ctccaggctc atggttacgg
     4261 cctttacgcc ctctacggcc tccagtgcct tctgtacggt cccggtgcag tggttgcaca
     4321 tcattccttc gatactgatt acttttgtca ttgtgtttgt cctctctttc tcttctttgc
     4381 tattttgctc tgcgggggct tccagtatgc actgttctcc ctccacagct gcctgcgttg
     4441 tctcctgttc cgtccgggct gcttccggcg ttcctgtttt cagcggagta aatccgctct
     4501 taaagcctct cagccgcagg gcatttccca cgacaaagat actgcttaaa ctcatggctg
     4561 ccgcgccgaa catgggattc agacggattc caaaagacgg gtacagcaca ccggctgcca
     4621 gagggatgcc gatgcagttg tagaagaaag cccagaacag attctccttg atgttgcgga
     4681 tcacggaacg gctcagtttc atggccgtca cggcatcccg cagatcattt ttcatcagaa
     4741 taatatctgc gctctccatg gctacatccg tgcctgcacc aatagccatg cctacatctg
     4801 cggctgccag tgcgggggca tcgttgatgc cgtcaccgat catggctacc ttatggccct
     4861 gacgtttcag ttcgctgacc tttttctcct tatcctgcgg cagtacttct gccacgacct
     4921 cagagatacc cagcttctgt cgtacagctt ctgcagtacg gcggttgtct ccggtcagca
     4981 tgatgacttt gacacccatt ttctcccaga cacgaatagc ttcataagaa gtggccttaa
     5041 cagtatccgc cacgccgatg actcctaaga atcttccttc ctgtgccaca aggagcggtg
     5101 tcatgccttc gtccgccatc cggttcagac cttctgtggc ttcctccggc agagtgatat
     5161 gattctcctg cagaaaggca aggttaccta catagaattt tgtaccggca ggaattatca
     5221 cgctactggc acccgatact gtatttctcg ccatctggtt ctgttccgtt gtcttgccag
     5281 agccatctgt ggtgtcgttc tgttttccgg cataaaatac cgccgtaggg ccgtcatttt
     5341 ttactgattc taccgcgcgg aattctgctg ccagtgtacc ttccacgcca cggccgaagg
     5401 ttgccttgaa atccacgatc tcgggaattg ccagaccgaa ctgctctgcc cattccacta
     5461 cagcttccgc cagcggatgc tcactttttt tctcaagagc tgctgcgatc tgcatcatgg
     5521 catccatcgg gaagtcactc tgataaccgc ctgcttcttc tacctttaac ttaccgctgg
     5581 tgatggttcc ggtcttgtcc agtaccacag tgtcaatctc tttggcctgc tgcagggcct
     5641 cacccgtctt gatcaggata ccattgctgg cacctacgcc ggttcccacc atgatagcca
     5701 cgggtgttgc cagtcccaac gcacaggggc aggaaattac caatacggca atggctgtag
     5761 aaatggcaaa ttccgcggta gcaccgctta tcagccacac gatcagggtg atcagggaaa
     5821 tagtcatgac cgtcggcacg aatacaccag ccaccttatc tgccagctgt gcaatgggag
     5881 ccttgctggc actggcctct tccaccagac ggatgatctg tgccagtgtt gtgtcatcgc
     5941 caacacgatc cgcacgacat tttaagaagc ccgccttgtt cacggtagcg gagaccacat
     6001 ggtcacctgc ctgtttctcc accggaacac tttcaccagt gatagccgct tcatccacac
     6061 tggagttgcc ttctaacacg ataccgtcca ccggtaccag acttccgggc tttaccagaa
     6121 aaatatcgcc tttctgcagg ctctccgtgg gcacttcctt ttcctgtccg tcctcggtca
     6181 caagaattgc caccttggga gccagattca tcagcctggt gatggcttca ctggtctttc
     6241 ccttggaacg gctctccaga tattttccaa cggtgatcag agtcaggatc gtaccggagg
     6301 attcaaaata cagatcgtgg gcataatgct ctaccactgc catgtcacca taacccaggc
     6361 cgcagctgat ccggtacatg gcgaaaatgc cgtacaccag tgccgcgccg gatcccagag
     6421 cgatcaggct gtccatgttc ggactgcggt gcgccaatgt tttgaatcct acagagaaaa
     6481 acttccggtt cacatacaga atcggcaata acagtaaaaa ctgcgtcaat gcaaaattca
     6541 tggcattggc attgccgtgc atcgtgttta cgataaatgc cggaaccgga attccgaacc
     6601 attctttcag catatggtgc atagacacat acattaacgg gatcaggaat ccaatggaga
     6661 ttcccagacg ccatttcatg gctcttgcgt ctgcatccag cttctgctgc agctcctgtt
     6721 tgccatcggc tgtggacttt cctgtacttc ccgcattccc ggaagtgctc tgtccgcctg
     6781 cacctgccac actgccttcg gtcattaccg aagcaccgta gcctgctttc accacagcat
     6841 cgataatttc cgccgaagtg atcttcgtct cgtcataggt cacatccatg gtctctgtca
     6901 gtagattcac ggatgctttt tccacatttt ccagcttgtt tactgccttt tccacatggg
     6961 aagaacatgc ggaacaggac attcccgtaa catgatatct ttccttcata atacccttgc
     7021 ctttccatga cctgcaaagt ctccggtgct ttcaatgcac cgcagcagca caaatttaca
     7081 ggtttatctg cgaccattcc gccgcttatt tcaatttctt cacaagttcc actgcctcat
     7141 ctactacagc agtattcccc ttctggatct cttccaccac acaggtctcc atatgttcct
     7201 tcaggattgc atagccaaag gcctgtaaag cactctccac ggcagctacc tgtgtgagaa
     7261 tatcaccgca gtagcggttc tcttccagca tccggctgat accgtttaac tgcccggcca
     7321 tgcggcttaa acggttctgt aattgttttt tttctttttc ggagcggggc gttgccttct
     7381 ggtgacaatg acaacattcc atgccttttt cttccatttt ttcttccttt tgttctgcca
     7441 taattttccc cttcctgagc ctccggcacc ggaatccgaa ttttccagaa tcttcccggt
     7501 tctacacata tgctgtttcc ggcctgaata tcagtgcacg caacatgctg ccggatcttt
     7561 cgttgcagcc atacgcaaaa taccccgcag gggtatcttg cctttttcta caagatatac
     7621 ccccataggg tatttgtcaa gaggcacttt ctaattcctg ccagttcatc tctgaccaaa
     7681 acagatcctt acgatctgca tcttcatgat tctgtcgccg gttctggcga ggaagcggaa
     7741 taatccgctg acggaagggt ctcgaagatc attatagccc agcatatagc cacggcttgt
     7801 gacctgcaat ctgctgtccc acggggaaat ctcacttttc gcatccgaaa ccgcaaaata
     7861 cgcacccaca tcctgaattt tagcgcttct tagccatgtc tttgctatca tcttaaccgc
     7921 tgtccggttc gccgcatcaa ataccagcac accaccggga aaactgtcag ccattgcctg
     7981 taccagcgtc ctgacttgct ccgtcagaaa ataatagaat actccggagg caaaaaatat
     8041 cgccccttcg gacgcgtcga ttttggtaaa ccattccgta tcattcaaat tgcagggaat
     8101 attttcctca cggtctccgg cgggcagtaa ttgattgcgt acggcaataa catccgggaa
     8161 atccagattg taaattttac agctgccatt gtcacaggct ctgccggtac cgtcaagacc
     8221 acagcccagc ttgaccaccg ccgcattggg atggtctgcc agata
//
LOCUS       DAWDHD010000108         8041 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_523_length_8041_cov_12.114075, whole genome shotgun sequence.
ACCESSION   DAWDHD010000108 DAWDHD010000000
VERSION     DAWDHD010000108.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 8041)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8041)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8041
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_523_length_8041_cov_12.114075"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1796 a         2377 c         1694 g         2174 t
ORIGIN      
        1 ccgcccccag ctgcccatag cttttcttgt cacatccgga gtgtaatgcc attttccggc
       61 atatccggca atttcctgct ttcccagcca ttccgtcagc ccttcctggt tctctgcgta
      121 agaaaaacag acaaaaggca ctcccagaac tgacaattca tagattgtcg ttccaccggc
      181 agtcaccgcc agctgacagg ccgtcatcag tcccgccatg tctttcacat cgcaatgcag
      241 aaccacgttg ttgtgctccg atgcatattg ctccaatttt tcccggttcg gattaaacct
      301 tcccaccacc agatgatacc ttatattttt tcccgccaga ctgtgctcac acagttcccg
      361 caggatttct cccgcaatgt tgtctttgtc tccgccaccg gtggtgatca ggatatccgt
      421 tacatcatcc tgcaaaacat actgtgctgc cgcctcacag aactgctgcc gcaccggcac
      481 ataagcagct ccgagatact gtccggtact gcttccatac agttcccgat acttttcccg
      541 ggaagcaaac agattgtaat tgatcacacc gtccaccgga aatgcatgct gctgcatatc
      601 atccagcaga tacaccctgc caaaccggtg caatgcctgc aaataagcat ccgtcaccgc
      661 atagctgtcc accagaaaaa catgcccgct tccccggatc agtccttcca gcgccgaaag
      721 ctctccttcc aggtcggcag gatccgtccc aaggatcttc acctgcatgt tccagctctg
      781 caacagttcc gcagatgcct gctccgcgca caaaaagcag agttcttccg gatgctctat
      841 tcttttttgt aattcctgtg ccaccgtgag gcaacgcatc agatgccctg cccccgtaac
      901 agcactgccg tccgcacgaa aataatacat ttccgctcct tactgctgtt ccttatctgt
      961 gaaatccaca agagaccagt ccataggggt tcccagcctg gcatctctgg tgatccgttt
     1021 gcccaacagt tcctcataat atctggtatg cagtccgcag gaaggtctca cagaccgcag
     1081 attctccgga gtaaacacat ccccggcctt catatcctgt gccacgaaca gggaacggga
     1141 atgttctctg ctctttttct gcttcggtgt cagatcgtag gtcacctttc ctactgcttt
     1201 ctcgatcatg cggatgtgat ccaccatctc tttgaattct tccggttcca tggagaatgc
     1261 cgcatccgca ccgccgtctg ctctgcgcag tgtcagatgt ttctccacca ccttggctcc
     1321 cagcgccacg ccggcaccgg ccacggcaca gcccatggta tgatcagaaa gacctaccac
     1381 acagtcaaag acttccttca tggaaggaat cgtcttcaga ttaatatcct catagggtgc
     1441 cggataagcg gaggtacatt tcaacaggat cacattctcg ttgccctctt ccttacaggt
     1501 acgcagtgcc agctcgatat ccgccagata ggcaattccg gtagacataa taatcggctt
     1561 gccggtacgg gcgatcttgc ggatgaaagg aatgtcgtta atctcgaagg atgctacctt
     1621 gtataccggt acctccatct cttccagaaa atcaatgctg gtcagatcaa agggagaaga
     1681 aaagaatacc agtcccatct cctctgcgat ctttttcagc tttggctgcc actcccaggg
     1741 agtataggcg gtctcataca acttgtgcag tgtggttccg tcccagatgg ttccctgtgt
     1801 gatctggaag cagggatcat cacagtccag tgtgatggta tccggggtgt aagtctgtat
     1861 tttaatggca tccgcaccgg catcctttgc cgcctgcatg atagccactg ctctgtcaaa
     1921 atccatcaga tgattcgcag acatctctgc aattacaaaa gtgggagaat cttcactgat
     1981 tttatgattc ccgatcatga tctctttgtt cataatagtg cctccttaag gaaatacaat
     2041 atgttcgtac ttcttgctgt cccggatccc tttccagtgt tccgctgtca tgcgcatata
     2101 tgtcacgtca taagcagctc cgtttttcac cacgtgggct tttttctctt ccacgacttc
     2161 acagccgcag atcttatgga gctggatgac tccctgattc agggtgaata catcactgta
     2221 taccgcattt ttcccaagat ccagaaacgc atggtcatac atactcatct ccagcgccag
     2281 tgccgtctgg atgctgcgca gttttttctc gcccacgtaa taggcccatc ccagatctcc
     2341 tgtgggattc gtcagccctg tgtaattgat gacgccggct gctgtgccgt cgatctcaat
     2401 gatccagtag ctcacatccg gatcctgctg cactttggca aaccatttct tctgcccttc
     2461 cagagtcagc ttcggatccg tattcatata gcgggtaatc tccggatcca tgcgccagcg
     2521 catgatctgt tccaggtctc cctctgtcac ttttcttaat gtaacactca ttcgcagccc
     2581 gccttttcca acagttcacg cattcctttt aactcaatgg cttcgtctaa gggaatgctg
     2641 acaccaagct cttcctccaa ctgtccgatg atcatgacat gcgccaggga atcccactgc
     2701 tccacatctt cgatcatggt atcctcggtg atggttcccg ccggaacctt taatacatct
     2761 tcaatcagct ttatgatctt ctctttcata tggttatcct ctgctcatcc tcattttttc
     2821 taacatttac cactgtccgg tcttccgtcc ggcagccgtc acccagcccc aggtcaatcg
     2881 tataaagtct ccttcagcca gtaggccctc tccggtctct ctgccagtgt gatctcatag
     2941 caggtaccgc cctcctgtgt actttccgtc ctgtggtaac ccagcttttc atacagagct
     3001 tccaccggct tattcttggc tgtcgggata tatctgccgc ggagtccggt atatccttcc
     3061 tcctgcatct gctcctcgat acggtcgatc accgcattct cgatattcct tcccatgaca
     3121 cggcagctca tgaccagatc cgtgatctcc ggcagttctc cgtcagtatc cacgatggct
     3181 gctgccacga ttccgttgtc tccaaacgca tccgtcacct gatacagaaa tactctcttt
     3241 ccggcatctt ccacgatctg ctgcatctgc tctctggtgt agcgtctggt ggtcaggttg
     3301 aactggtttg ttttattcaa aagctgtgtc agccggtcca gatgttcctt cggatccacc
     3361 gggatcagac agatctccag ctttttcaca tagtcttcaa agctcaccgc ctgctctgcc
     3421 atggcatttc tgccggcatt ggcccggtac tgtgcggtct tctcccggtc ctctttcgtg
     3481 atcactgccc gggcaaaata ttcttcataa atctttgcca tgcagggcgc cagttcctcc
     3541 ggtcttgccg ggaaatcggg cacagtcacc tccggcagca tctgccgtac catttcccgt
     3601 tccgcttcgc tgtcatcaaa aaagacaaag gaatccgttc ccagattcag ttcttccgcc
     3661 atcttacgga tattgtccgg cttcgggtcc cagttgatcc tgcgggctgc gaaatcttcc
     3721 ggcccaagca gcatatgggg atgatgttcc aagatctcca tggcatcctc ttcattattt
     3781 ttggatgcta tggctaacag cacaccctgt tcctgcatca gctttatgac ccgttgcaga
     3841 tttttataag ccagaccgct gtggtcttcc gacaaaagca ccggtgtgtg atccgtctct
     3901 ccggccagac cgccccacag ggtattgtcc agatccagga ccagcacctt tttaggggtg
     3961 cgttcctcca gttccgcctg ctgtaatatt ttttccgcca gaagagactg ggtctccatc
     4021 cccagcaata cttttcccat ataccacatt ttcagggaaa acgccttctc ctcgccctga
     4081 tgctctatga tccggcgata ggggaatatc ctcacattcg catgcttcac cgcaagcttt
     4141 tgcaattcca tactccacag gctctccagc tgctgtttgc gttccggatc cgccagcaca
     4201 gccagttcca ccgcccacag atatgcatcg gagacataga acacaccatg ctccggcaga
     4261 cagccttcca gcgtctgaaa ccagcttccg atccgctctt ttgctgtctg cggatcatag
     4321 tcatgttcca gcagttctgc cagatccatg atcagaaagg taatgtccgg ctgaaacgca
     4381 tgataggagg aagccgggtt cagcaaagct cccagttcat ttccatagcc ttccgcatcg
     4441 tagacttccg cctgtttctg taacatacgg atcaccatat tcatatttac attggacaat
     4501 actgccagtc tgcctgttgc catagtctca gattcctccg ggattatttt tcctccgtca
     4561 tcagacggta tttcatctct gcgatcttcc agtccgtcag attatcgata tcctgcactt
     4621 ccagttccga tacgatcagg ggcagaatat ttcccaccat cagcttctta ttcactgcaa
     4681 agcggtccgt acggaagaca tagaactgtc ccacatcatg ataatggggc tgcaggtcct
     4741 gggatctgct gtccagatat tcgggatatt taaataccaa ccgttcctgc tccactacca
     4801 tggcccgctg gggcgggtag gaaaagctga ccaccggaat cagggtgtcc gcatcgcttg
     4861 cttccagctg ttctaccgct gctttcagtt tttccgctgt cacaaaaggc gctgtgggat
     4921 agatgcagca tgccacatca aaatgcatgt cacgcttctc atattccgcc aacacttccg
     4981 ccagcacatc attggtggtg gcaaagtctc cggaagtcgc ctcactgcgg aaaaacggca
     5041 cctctgcacc gtactttttc gcaatctccg caatctccgt atcgtccgtg gataccatca
     5101 catggtcaaa aagtctgctg gacaatgccg cctcgatgga atatgccaga atcggttttc
     5161 cacaaaaatc tctgatattt ttcttcggga tccgcttgct cccacctctg gcggtgataa
     5221 tcgctaatct gctcattttc ctaacctcat ctttctcatg ccccatggcc gcccttcagg
     5281 cccgtagctt ccatacaaac tgccggatcc tgtaatagag atagaatcgc agatcgctct
     5341 ttttctgcat ggcgatgaat ttgcgttcct ccggcccggt atattccaga taatatttgt
     5401 tcctgtcgaa ctccggaaaa tgtcgttctg tcccctcccg tagttctttt acaaaagaca
     5461 gcttcggctt ctccactccc gacagatagg agaataaagt gatcacataa tacagctccg
     5521 taaaacggta ttcgatctgc tcccggtatt ccgcaagaaa ccctctgttc tcgcattccg
     5581 tgtacaaaag ttctgctgct ttcatacgat cacggcattt ttcctccgtg atgtgatgta
     5641 ccgtggacac cgcatgctgg taataataat acagaggttc ctccactctc tcaaaacgtc
     5701 gaaagtacag gctccacaag gggcctgcac agttgtcttc gtagaaaata tgctccggaa
     5761 aatccagatg attctcccgg atcacactat gtttgtaaat tttcagcacc atactgccgg
     5821 gacgcatgaa cagtctctca tgcttttcct tcgtcagatc cccggtctga tccggtgtat
     5881 tgttgacgat gaccttcccg acctcaaagg tatgatgatc caccagactg taatcgcagc
     5941 ccaccagatc cgctccggtt gcttccgctt ttttcagaag cttctcgtaa tagtccggtg
     6001 acacccagtc atcactgtcg atgaaaccga tccattcccc ctgcgccacc cgcagtccgg
     6061 catttttggc accgccctgt cttaagttca cagggttcgg aatgacacag accagctccg
     6121 gataacgctt ctgataatcc agcaggatct ccagagaatc gtctgtagaa gcatcatcca
     6181 cgcagaggat ctcataatcg tacagtcccc ggatggtctg tcccaccagg ctgtccagac
     6241 aataattcag ttttccctcc gtcgccatat tatagacggg cacgatgatg ctcagttttt
     6301 tactcatagg cgttacagat ttccccgttc tatttttgct ctgccgtctt ctacccgata
     6361 gacaatgtca catttttcaa tggtctgcaa tctgtgggcg ataatgatca aggtcttgcg
     6421 gccgtgcagt cggttgatag actccatgat ggctgcttcc gtatcattat ccagtgcgga
     6481 agtggcctca tccaggatca gtacctccgg atcattatac agcgctctgg cgataccgat
     6541 acgctgtctc tgtcctccgg agaggcggat gccccgctcg ccgatgccag tctccagacc
     6601 ttccggcaga gtcttgataa attcatccag ctgtgcctct ttcaatactt cccacagtct
     6661 gtcctcatca atcttgtcct ccggcacacc gaaggctaca tttttacgga tggtgtcatc
     6721 cagcatgaag atcatctggg ggatgtagcc gatattcttc agccatttcc ggtaattgcc
     6781 cggttccttt acatccacgc tgtctgcata gatatgtccc gtactgggct ccagcagtcc
     6841 caacagaata tccaccacgg tactcttgcc cgcgccggaa gtacccacga tacctaccga
     6901 tgcacccacc gggatcttca gatccgcatg gtcgaagatc agacgctccg tattgggata
     6961 ggcataagta atatctttca tctcgatgct ttcttttaca tccagctttt catcttcatc
     7021 ggtagcaaag gacatgtcga tattctgtcc gttgatctca tcctgtaaat tatcgctgac
     7081 acccatgaag aaaggttcaa aataggcgat ggaattgatc tggttgttga tacggttcac
     7141 gcagggcaac agtaccagtg ccgctgccgc cagagttccg aacactgtca gcatattttc
     7201 tgtaggcaca ccggtggcca ccagaaagat catgtatcct accatagtgg caacgcaggc
     7261 tgtctcgatc agcagcttcg gcacctgatt gtacaggctg taacgctgta cggcatccac
     7321 gtagcctttt ccgcatttgc ggtactcgga tacgaaatac tgctccttgc cggaaatctt
     7381 tacttccttg ataccctgta ccgtctggga tatccatttg aacagactgc tgtaatagtc
     7441 ctgattgtcc ttacctgcct tgtacatgat gggcttcagc actctcttga tcagccacat
     7501 caacagaagc aataccaccg ccatcaggat cgtcatggta ccgctggaaa taaagcagta
     7561 gctgatgacg aacaacgata ccaccgtatc ggacaacagc tgtaaaagtg ccaggatcaa
     7621 tgcatacata ttgtttacat ccgatgtgat gcttcgctgc accaccgcag tatccgcatt
     7681 cagataaaac tcgtaacctc tgcgcagata gttgcgcatc atgcgctccg aggtgcggaa
     7741 ctggttcgtg tatacgaaag ataatgtcaa cttctgctgg aaataagtaa acaggttctt
     7801 cactacgaat atcaggatca gaagtaccat gacaataatg gagaacgtac gatagctctc
     7861 cacacccatg atctcatagc aggcttccgc cacccggctg tgttgtaccg cttcgggatc
     7921 aatgacgatc tgcatgacct ccaccagcat tcccacaccg gcagtctgta aaaaggcact
     7981 gatcaccatg aggataatga gtcccaccat ggttcttttc tgttttttgt ccagcaggac
     8041 a
//
LOCUS       DAWDHD010000109         7995 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_528_length_7995_cov_9.913602, whole genome shotgun sequence.
ACCESSION   DAWDHD010000109 DAWDHD010000000
VERSION     DAWDHD010000109.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 7995)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7995)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7995
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_528_length_7995_cov_9.913602"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1890 a         2203 c         1683 g         2219 t
ORIGIN      
        1 gcataggctg ccttaggcgc ataatagaag ccttcccgat agccccaata gttcagcacc
       61 cggtagccgg gcaatttacc ttctccgttc ttttcccccg ccacggccgc aagttctccg
      121 gaggtcttcg gtaccgtctt ctccggtgtc tcatcgaatt catagaccgg ttggaattcc
      181 acggttgtca caccgatttc ctgcagatag tccagcttct ccacaacccc tgcaaaggtg
      241 cctcgatgtg ttacctgaga tgaggcatgc atggtaaagc cacgcacatg aaggcaatag
      301 caggtgctgt cttcataggg aagcataggg tgcatatcct gttcccagtc gaaatcttcg
      361 ttgggaaata ctgcaatgcg atccgcaggc ttacgtattt tcccatacgc aggcttacgc
      421 aggaacccct gtgcatgcag atccggcaca gtacgctccc cttcatagaa ctgatacccg
      481 atattttccg gctccagttc cagatattta cagtacacat ttcccatccg ttcctcctgt
      541 cgaaacggaa ccctgtgcgt tttttttcca ctctttctgt catatatgag aatgccgcaa
      601 tcctttttcc cggacgcata ggcaaaacgg atggctccgt cctccacatg cgcccccaga
      661 gggtaaggct ttattgcatg ctttttttct gtcattgtca taccccatct tccagttttc
      721 tatcacttac agtctctttg tccattttta ttatagtctg taagtgtaag aaaaacaagc
      781 attgcaagaa tatccgtaag cggttataat aaaggaacaa agtactgtta cacccaataa
      841 acataacagg aggattccca tgggtaaaaa agaaagctat gaagatgaag aaatgaccgt
      901 tgagctggag ctggaagacg gcactgttgt caactgtgcc atcattacca ttctcaccgt
      961 agaggccaag gactatatcg tactgttgcc tctggacgag gacggcgaaa acgaagacgg
     1021 agaagtatgg ttctacggat attccgagaa tccggatgac ccgaatgaag aacccattct
     1081 cgcatacatt gaagatgatg atgtctacga aaaggttgcc gatgcttttg atgagtatct
     1141 cgacagctgt gaatttgacg agatcatcga tgataacgat tcagaggaat aaggaatctg
     1201 gaagggaacc tgggacgctc tctcgtgcct gtaaggcaga gaagttccag gtcttttttt
     1261 gcccgtgtca tcgccacata gaagatccgt cgttcctctt cacaatgctc cggctcccgg
     1321 gagcttccgt ggggaaaggt cttctcatta cagtccggga tccacacatg gtcgaattcc
     1381 agtccctttg ccgcatgtac cgtcagcaga gtgatcccgc tgttttcctg tttttcctgc
     1441 ttcttctgca tgccactcct tacagtttcc ctgcattgca gttgttcccg gtaaagctcc
     1501 tgtgcttcct gccattcttc aaggcttcgg tatcgtcccg catcctccgt gataaaatcc
     1561 agcacttcct gccattccgt cagatctgtt tttccctcct gtgcagctct cacccgcagg
     1621 tatctctcat agccgcacgc cttacgcaaa aatgtcattg caagcttcag ttccgtattt
     1681 tttacaaatt ccatctgtcg tatccatact ccgaccgctt tctgcgcctt ttgctgcccc
     1741 gcgtccggac ttcccgcagt cagatcttca taatatttct tcaaagccgg taaagatgct
     1801 tcggcaccaa aagcctctct ggaaatattc cgatatggtc tgttgctcac cctcaggtaa
     1861 tccgctcttg tagcacaccc ctgcgcgatc cgcagatagg acagaagatc ctgtgcgata
     1921 aaatggtcgt aaatgtttct ccccttctct ttcatggaaa aagggatccc ctccccggta
     1981 agtctcgccg ccacgccctg catggccaga tttgtccgga acaatacagc acaggtctcc
     2041 ccatttatcc gatttttcaa gctttgtacc agataactca tttgttccga agcctcactg
     2101 aattccctga tttttacccg atattccttc cggtatccgg cgtcagtctt atgaccccct
     2161 gcctgccgtc tatgcgcctc gcagggctcc agatccttat gaaaacggtc tttattctca
     2221 tctatcaccg ccagggatgc ctccacgata tccggatgac tacgataatt cgtccgaaga
     2281 aggatctgct ttgcaccaaa ttcctctaca aactgtctca tgcaggctgg tctggctccg
     2341 cggaatccgt aaatggcctg atcatcatcc cccactgcaa aaatatttct atgtttttcc
     2401 gccagaattt tcacgatact gtactgtctg taattgatat cctgaaattc atcaatcagt
     2461 atatattcaa accggttctg ccagtatgta cgctgccccg gatccctttg cagaaactcc
     2521 tcacattcct ttaatatatc ctcaaaatca aggcctccgg tattttttct cgcctcctca
     2581 taagcttcat agatcctgga aaaaagttgt ttttcttctt ccggaagctt ctttctgctg
     2641 ttctcaatat cgccggtatt tttgtaataa ccgatggcag caagatagtt cccggcaagc
     2701 tccgttactt ccgctgcgga tcttttttta cagtctgtta ttttcttcaa aatgggaagg
     2761 ataagatctc ttttttgtct gtcgtttaaa atattgcctg atgaaattct gcccgactgg
     2821 agcaggattt gatagaaaac agaatgaaat gttccaaaat tgacggtatg cacttcctgt
     2881 ggagatatct gcgcaaagcg ctgcctcatg gatagagccg cttctctggt aaaggtgatc
     2941 accagaagct tctccggggt gatccggcgt tcccggatca gatacaggat cctctgggtg
     3001 atggtaaatg tcttgccgga gccggggccc gccagtaata acagcggtcc ctctccgaac
     3061 gtgacagcct cccgctggga ggcatttaag catgtcagat cagacatttg cctgtaataa
     3121 ctcgattcct ttgcggattc ttgccagaga ttcctctttg cccaggactt ccataatctc
     3181 cgtagcaccg gcaggtgtca tctgcttgcc ggatacggcg gtacggatcg gccacatcac
     3241 atagccgttc ttacagcctt tttcctccac atattttaca agagtctgat acagcccgtc
     3301 attgctgtaa tcctcctgtg cctccaggat cggaagcaga tctttcaata cctccagaga
     3361 agtctctgcg gtagtcttca tcttcttgtg tgtatacatt gccacgtcat attccggaag
     3421 ctcctcgaag aaatctacca gtccggcaat atccgggaat acttcgattc gggtctttac
     3481 catggctgcg atcttcttca gatccagagg cttgctaatc gctgccttca ggtaaggctc
     3541 tgccatctca tagaacttgt cgaaatccat agccttcaga tattcaccat tcatccaacg
     3601 caatttcacg atatcgaaga ctgccgggga cttgctgatg cgatggtaat cgaactcctt
     3661 gatcaattca tccagagaga aaatctcgcg gttatcctcc gggctccatc caagcagtgc
     3721 cacatagttc acgatggctt cggtaatgaa tccctgatcg atcagatcct cgaaggaagc
     3781 atgtccgctt ctcttggaca gcttcttgtg gttctcatca gtgatcagcg gcagatggat
     3841 atataccggt acctgccatc cgaaagcttc gtacagacgt acatattttg gggaggagga
     3901 cagatattcg ttgcctcgta ccacatgggt gatattcatg gtatggtcat ctaccacgtt
     3961 tgcaaagtta taggtagggt aaccgtcgga tttgatcagg atcatatcat ccagctggga
     4021 gttatctact gtaatgtctc cgtataactc gtcatggaaa gtcgtggtgc cttccttggg
     4081 gttgttctgg cgaatgacaa aaggcttgcc tgcggcaaga tttgcctcaa tctcttcttt
     4141 ggacagggac aggcagtgct tgtcatatac ggtgatctcc ttaccatcca caacttcggt
     4201 cttcagggaa gccagtctct ccttgtcaca gaaacagtaa taagcttctc ccttatcgat
     4261 cagttccttg gcatatttca tataaatacc ggttttcata cgttcactct gcacgtaagg
     4321 accataaccg ccgtccttat ccgggccttc gtcatgtacc agtccggtct tctccagggt
     4381 acgatagatg atctcggtag caccttccac ataacgttcc tgatctgtat cctcgatgcg
     4441 gagcataaaa tctccgccgg catgctttgc aatcaaaaat tcatataatg cggaccgcag
     4501 attacctaca tgcattttac cggtagggct gggtgcgtat cttgttctga tctttgtcat
     4561 tttctcctca tttccgcagg cgatccgtat ccgttctgta ccttccggcc tacgcttccc
     4621 tgcttatata ttcctgtccg ccctctctca gaaggcagaa ctacttcctt attctaccgc
     4681 cttttttcaa aaactgcaac agttaagaca aggttttcgg atggggcaga ttccaccgaa
     4741 agtgtgccgc cagaaaccgg atcgccatga ccacaaaggc agcgataaat accgcgggaa
     4801 gtctgccgat actacggtac agccagacgc agcagatacc gcctaccaga gaggcacagg
     4861 cgtaaatatg ctttacgaaa atatacggtt cctctcccgc catcatgtca cgcatcagac
     4921 cgccgcccac gccggtgagc atgccgataa ataccagcaa aaaggtattg tccggatagc
     4981 cgttgtcgat gccagacatc acacccaggg aagtaaaaat tcccagtcct acagagtcca
     5041 tgcctaacat cgctttgtca taaaaggttc ccagtctgcc gctcaaaaga tcctgtttga
     5101 tataaagcag tataaaaata gccagtgctg tagccaccga gacaccgaca tataccggtt
     5161 tctccagtgc cgaaggtaat ttcccaagga tcacgtctct ggtcatacca ccgcctacag
     5221 ccgtcaccac gcccagcaca cagatgccga aaatatccat ccgtttccgg atccccacca
     5281 tggcacctga tgctgcaaaa gcgatggttc ctaagatttc catgaaaaat ataatactgt
     5341 cctgaatgtt cataaaatag tttctcctaa aagttctcca cgctatttta tagtatcttt
     5401 cccgaaataa caatatgcca tttgtaacta ttctgaatag tttctctaat tttatctcta
     5461 tgcgcgcaac aaaaccgcca cggtaatgtg acggtttcgt tttcgggata tatctgttct
     5521 gttgcagtgt ttattctttt tccagttcca ccatttcctg atccacactt tcatcaggca
     5581 ctttcttctc agcttcgttc ttgaacttgt ccacggctct ggcagctacg gggatggcga
     5641 tgttggtgcc ggctccggcg atacagcctc cggggcaggc catgccctcg atcaggcagc
     5701 cgttcatctt gccggccttg gcaaggagca gggctttctt acattctgcc aggctctccg
     5761 catgctggat attgaccggt gtaccgggat agtatttctc gatgcagtcc ttgatggcat
     5821 tggcaacgcc gcctgccaca ccataacctc ttccggcgga ggtggcatcc ttcatctcgt
     5881 ccatggcctt gatctcgtcc agcagaattc ccttggcctc gaacataccg gtaagttctt
     5941 caaaagtgat gacaaagtcc acatcggatc gaacggtacg tctgctggct tccagcttct
     6001 tggaagcgca agggccaatg aatactacgg atgcatccgg atgttcctgt ttgatcagac
     6061 gggccgttgc aaccataggg gttaattcat tactgatctt atcaatggtc tcagggaaca
     6121 gattcttcgc cattacagac caggaaggac aacaggaggt cagggcaaac tgctgcttac
     6181 cggtagcaac ctccttcacg tagtgctccg cttccgccat acatcccatg tcagcgccca
     6241 acgctgtctc atagacatcc gcaaacccca attccttcaa cgctgtcttg aacatctcag
     6301 gtgtcacctt cgggccgaac tgtccggcaa atgcaggtgc tacctgggct atgatcttcc
     6361 ggccggactg catggcacgg atcaactgga agatctgaga cttgtcagca atcgctccaa
     6421 aaggacagct taccatacac tgaccgcagg atacacattt ctcattgtcg atcacggctc
     6481 ttcccacatg gtcagatgta atagctccaa taccgcaggc agccttacag ggacgctctt
     6541 tatggcaaat agcatcataa gggcagatct cacgacattt tccgcacttg atacattttt
     6601 cctgatcaat gacagatttc ccattcttca tggaaatcgc acccttgggg cagacactct
     6661 ggcagggatg tgccagacat cccatgcagg aactggtcac ctcgtaacgg ttctccgggc
     6721 aggcattgca tgacgaagga atgacctgca tcagaggcgg ctcataatat ttttcatcaa
     6781 tattactctc ttccagcccc tgagtcagat gaccgggatg ctcacggttc tccgggcgta
     6841 agctcatacc catggccaaa cggatgcgct cacggatgat cgctctgtca cgatagacgc
     6901 tctcccagaa ttccgactct tcataatcta cgatcttata aggcagcgcc tccatatcat
     6961 ccttcagatt tgtggaattg taagccagat tggccacttc cttaaatacc cttcttctcc
     7021 gctgtcttac cggagtatcg atccctctca tgctgctcat atcataatta ccgccttttg
     7081 atattttttt aacacactaa ccatattaaa ccatatcata tccataaatg caagaaaaat
     7141 atcaataaaa atgggatctc acaccgtttt tacgtgaaag atcccatcct tagaattttc
     7201 tgttattctt atcttttatt acttcataac gactctcttg aagttcttct tacctctgcg
     7261 gactaccaga ccctctccgt caaactgctc gggatcgtaa gcggtcttca catctgtaac
     7321 cttttcatcc gctacgacaa caccgccctg ttctacggca cgtcttgctt cactgcggga
     7381 tgcggacagt ccggatttca ccagaacacc gagaatatca atcttaccat cggtaaagtc
     7441 tgcctctgtc agctcactgg taggcatttc tgcggcgttg ccgccggtaa acagtgctct
     7501 tgcactctcc tgtgccttct tagcctcttc ctcgccatgt accagattgg tcagttcgta
     7561 tgccagaatt tccttcgcct gatttaactg gcttccttcc catttgtcca tctcatcgat
     7621 ctgctccaga ggcaggaagg tcagcatgcg caggcacttc aggacatcag cgtccgatac
     7681 atttctccag tactggtaga actcgaaagg tgtagtcttg ttgggatcca gccatacggc
     7741 acccttctgg gtcttaccca tcttcttacc ctcggaattt aacagcaggg tgatggtcat
     7801 ggcataagca tccttaccca gctttctgcg gatcagctcg gtgcctccta acatgttgct
     7861 ccactgatca tcaccaccga actgcatgtt gcagccgtat ttctggtata aggtcaggaa
     7921 gtcaaagctc tgcatgatca tgtagttgaa ctctaagaag cttaagccct tctccattct
     7981 ctgcttgtag cactc
//
LOCUS       DAWDHD010000110         7949 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_531_length_7949_cov_11.148467, whole genome shotgun sequence.
ACCESSION   DAWDHD010000110 DAWDHD010000000
VERSION     DAWDHD010000110.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 7949)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7949)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7949
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_531_length_7949_cov_11.148467"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1613 a         2446 c         1684 g         2206 t
ORIGIN      
        1 gcggtattca gtgcaaacaa aaatccggaa caggccgcat tcacatcaaa agccaaagca
       61 ttcaccgcgc cgacagctgc ctgtacctgg caggcacagc agggcagata ctgctccgga
      121 gaacaggttg ccaccaggat caggtcgatc tcttccgctc tttttcctgc ctgttccagc
      181 gctttgcggg cagcttctga cgcaagtgtc acaaccgttt cgccaactga cacatgtcgt
      241 tccgcaatac cggttctctc ccggatccat tcatccgagg tttccaccag ctgttccaat
      301 tctttatttg taacgatcct tcccggaagt gcacttccgg ttccggtgat tcttatggtt
      361 ctcacgccat ttcctccatg atatccactg ttctttctat tttctgcgcc cgccaggcat
      421 tgctgccgca gaacagcagt ccgttatcca catctcccat tgccgcattg atcaacgctc
      481 tggtgatgca atacggtgct gtcttcggat cgcaggtttt aatgcaatgt ctgcatccgg
      541 tcataaaccg ctccccggcc gccacctttt ccagaaaagc attctgtata gctctgcccg
      601 gcattcccac agggcttttc acaatgacaa tgtcttcttt ccgtgcgctg agatatgcct
      661 gcttatatgc atctgccgca tcacactctt cggtggtcac aaaccgggtt cccagctgta
      721 cccccacagc tcccagggac aggcaatgct ccagatccct ccggtcataa ataccgcctg
      781 ctgcaatcag tggaattccc agttctgctg cctgccggat cattgccgta agttctctct
      841 catagttctc cggtgtgtaa gcatgcagtt attcccgcta aaatcccaga tgtcctcctg
      901 ccaacgggcc ctctgccacg agaaagtccg gctttctgcc gtattttttc atccagtatt
      961 tcgtgacgac cgacagggca cgggtgctcg acacgatcgg agccagcatt gtccggtggc
     1021 tgcgcccggg cattccgttt tccgtctctg ccacaactcc gggcagatcc agcggcagcc
     1081 ctgcaccgga aataatacag tcggtacctg ccaacactgc tgccctgaca taatcctcgt
     1141 agcgctgtgt tgccaccatg atgttgacac cgacgatacc gcctcttgcg atctgccttg
     1201 ctttttctat ttcttttccg atagtgtgca gattacaggc gatgggatct ctgtcaaaat
     1261 ccggctcccg gaaaccgatc tgggcagtag aaataatgcc gatcccaccg gcagctgcca
     1321 ctgctcccgc cagaccggac aggctgattc ccacacccat gcctccctgg atcacgggaa
     1381 tatctgcatg taattccccc aggatcattt ttccgtgctt catcccacat cacctctcgt
     1441 taccgcttta atacttgcgg aacctttcat aacgttcctc tgcgatctgt tcaccggtca
     1501 tgccggtata tttttgcaga aattccttga tatgttcctt gagatacccg ccgatggctt
     1561 ccacggtgct ttggtctgcc ccgccgtatt ccggtacgat cctctcgatc actccgaggc
     1621 gtttcaggtc ctcggatgta ataatcatga cttcactggc ttctctggca cggtcagcat
     1681 ccttccataa tatagaagca aagccttcgg gagacaggat cgaataggtg gcattttcca
     1741 tcatccacac ctcattgcct accgccgtag ccagcgctcc accgctgcca ccttccccga
     1801 tgagaatgca caataccggc acttttaaag ctgacatctc cagcagattc cgggcaatgg
     1861 cctcgccctg gcctctctcc tctgcctcca cgccgcagaa ggctcccggc gtattcacaa
     1921 agctgataat gggccggttg aatttctccg cctgcttcat caggcgcagt gccttacgat
     1981 acccttccgg catgggcatg ccgtaattgc gttttgcgca ttccgtgaaa tccttgccct
     2041 tcacatctgc cagcactgtc accggctgcc cgtccaggaa tgccactgca cctaccagtg
     2101 ctttatcatc cccataataa cggtctccgt gagcctccac tacataatcg aaaatgtagg
     2161 gaatatagtc ggttgctgcc ggtctgtcca ctctccggac ggtcttgacc ttttcccatg
     2221 ccgttctggg gagtgtcagg aatgaccgct ccttcacaat ctccgtaggt tcaaaatgga
     2281 agtcaaaatt tttcttaaaa tccgcataat tcccctcact gcaccgatgc gtcgtgatca
     2341 ggaaatataa ggtcttcttc agattctccc ggcggacgat accgtccaca aaaccatgct
     2401 cctgcaaaaa ttccgctgtc tggaagccat cagggaggcg ctgtcccaga gtctgcttaa
     2461 taactctcgg tcctgcgaag ccgatcaagg ctcccggttc cgccatgatc acatctccca
     2521 gcatggcaaa actggccgtc acaccacctg tggtgggatc tgtcaaaata gtcgcataga
     2581 gaagtcctgc ttctccatgc cgtttgatcg ctgcggaggt ttttgccatc tgcatcagtg
     2641 acacgattcc ctcctgcatt ctcgcaccgc cggaacagca gaacaaaaat acaggcagcc
     2701 gcaattctgt cgccttttca attgcccggg tgactttctc gccgaccaca tgccccatac
     2761 tgcccatcat aaatttggat tcgcagatgc cgatcacagc cttttccccg tagatctcgc
     2821 attcacctac ggtcacagct tcttttacgc cggatttttc cctggcctcc gacagctttt
     2881 cttcatagcc ctcatatttc agtggattgc tctcttctat ctcctcgaac cagggctgaa
     2941 agcttttccg gtctgccacc atgcggatcc ggttgtgggt cttcacccgg aaataataac
     3001 cacactcata gcagacatat ttatttttcg ctgcccggtc tctgtttatc attttcttac
     3061 atttcgggca tttgatctct gtttctgtat ctacacttcc catacctgca cctcttattc
     3121 tgcgtcactt ttatctctct gtcccgctca taaaaaatca cttccgcaga ctctttttat
     3181 ttcaacgcaa aggtaagctc tgccttcgcc gccagttttc cgtttaccgt ggcgatcgct
     3241 tccccgacac ccaccggtcc cttactgcgg ataatggtgg tctccagctc caaggtgtct
     3301 ccggggcgca ccatctgctt gaattttgcc ttatcgatcc cggcaaaata agcagtcttg
     3361 ccacgatgct ccggcagaga cagaattgct acagcccctg tctgtgccag tgcttccatg
     3421 atcaacacgc ccggcatcac cggattcccc ggaaaatgcc cggcaaaata cggttcgtta
     3481 aaggtcacat tttttcgtcc cactgcccga acaccgggct ccagctcctc gatagtatcg
     3541 atcagcagaa atggatgtct gtggggcaga atctccatga tttcctgggt gttataaact
     3601 gacattgttc ctccgttccg cgaatgctgt cgtatcaact acttcctcgc attgtgcaat
     3661 cgtcctcgtt gatgctctgc cttctatcct gtgtcttgaa catctgctgc ggttattcta
     3721 tgtacttctt caacagcaga ctcgcattat gtccaccgaa gcccaacgaa ttggtcaatg
     3781 cataaggcac ctcttctttg tagctgtccc ggcagtaatt cagatcaagt tcctcctccg
     3841 tctcacgcag tcccacggtg cggtggatat agccttcctg aatttctttt acgcaggtga
     3901 caaactccac tgcacctgct gctcccagga gatgtcccac catggatttg gtggaattga
     3961 tcttcaggtc atacgcatgt tctccaaatg ccagcttgat cgctctggtc tcgaacaggt
     4021 cattatgatg ggtgctggtt ccatgggcat tgatataggt aagctcttcc ggtgccaccc
     4081 cgccgtcttt taaggcattc agcatggcgg tggctgctcc gctgccatcc tccgcagggg
     4141 atgtaatatg ataagcatcc gaagaacagc cgtagccggt cagttccgca tagatcttcg
     4201 ccccgcgcct ctttgcatgc tccagctctt ccagtaccac gatagcggaa ccctctccca
     4261 tgacgaagcc gtttctctcc ttgtcaaaag gaatagatgc ccgttccgga tcctcactaa
     4321 atgacagcgc tgtcagtgct gaaaatccgg caatgccgat gggggtaatg gagctttccg
     4381 ttcctcccgc tatcattaca tccgcatcac cgtactggat tgtacggtat gcttctccga
     4441 tggaatgtgt accggttgcg caggcagtaa ccacattcag gctcttgccc ttcagcccat
     4501 aggcaatgct gacattgcct gctgccatat tggagatcat cagaggcacc agcatggggc
     4561 ctaccctgcc ggggcctttt tccttcaggc ggtcatattc ccgctccatg gcctgtaaac
     4621 ttccgatgcc gcttcccaca ctgcagccca ccatataagg atcttcctgt tccatagtaa
     4681 gacccgcatc cgtgatcgcc tgtccggcag ccgctacagc gaactggcag aactgctcca
     4741 ttctgcgggc ggattttttg tccatatatt gtgcgggatc aaaatccttc acttccgccg
     4801 ccaacttaca acggtaatcc gcagtatcaa aataagtgat aggaccgaat ccggtcttgc
     4861 cctctttgat accattccag aattcctcta cgctgtttcc aatcggagtg atagctccca
     4921 tgcctgttac tacaactctt ctatttttac ccatacgtga tatactccct cgcgtcctga
     4981 cttattttct atcctgctgt actgttagcg gacatatttt ctattctgct ttactgttgc
     5041 cggaggtatt ttttacattg ccataccgcc gtccacgcac agcacctggc cggtaatata
     5101 gtccgaggca tctcctgcca ggaacagaac cgcctgtgcg acatcctccg gtcttcccat
     5161 tttcttaagc gggatctgtc caaccgcatt atcctgtacc gcctccggaa gcttatctgt
     5221 catatctgtc cggataaatc cgggtgccac ggcattcaca cagatattcc gggatgccag
     5281 ttctctcgct acacttttcg tcagaccgat cagtcccgcc ttggaggcgg aataattagc
     5341 ctgccctgca ttcccgacga tgccactgac agaagagata ttgatgattt tcccatatct
     5401 ctgcttcata aaggtcttcg tcagatgccg gatcatgtga aatgcgcctt tcaggttcgt
     5461 atccaggact gcattgaatt cttcttccgt catccgcacg atcagattgt cccgtgtgat
     5521 cccggcgtta ttgacaagga tgtcaactct gccgtatttc tctgttactg cctttacaaa
     5581 ggcttcactc tctgcaaaat ccgcaacatt gcacttcaca gcctccgcca tgccgccttg
     5641 gcttcggatg tccgtcgcca ccgcttccgc tgcttctgca gagccgttat cattaatgat
     5701 caccgtggct ccttctgctg ccaaggccag tgcaactgca tttccaatgc cccgtgatgc
     5761 tcctgtgaca atagcaactc gtcctaccag actttttcct gtcagcatgc cagctcctcc
     5821 atcacccggt ccagatcctc cggtttctcg atattgtata ctttgacatt tctgtcaatt
     5881 ttccgtacga agctgctgag tgtcttcccc ggaccgatct ctatgaaagt atccacgcca
     5941 tctgcgatca tccgctctac gctctgggtg aaatgaacgg gactggatac ctgttgtttc
     6001 agcagttccg caacctgtga cgcatctgtc acataatccg ctgttacgtt ggacacgtaa
     6061 ggaatccagg gggttgacag cttgacgctt ttcaattctt ctgccagctg ttctcccgca
     6121 gttttcagca aagctgaatg gaatggtccg ctgaccttta agggtacaca gcgttttgca
     6181 cctgcttctt tcagcgcttc cgatgctgct gctactgcat ctgcctctcc ggtgatcacg
     6241 atctgccccg ggcagttata atttgccacc gagacgactt tgccggtttc ttttgctgtc
     6301 tgtccgcaga tttttgccac ggtatctccg tccagtccta atattgctgc catggctccg
     6361 ccttccggat atgcctcctg catatagata ccacgcatcc ggatgatccg gaacagatcc
     6421 ggcaactcca tcacaccagc ggcagccagc gccccatatt ctcccaggct taagcctgct
     6481 gccatctccg ctttcagccc ttttgcctcc aatactttca gaatagccac ttccatggtc
     6541 agcatggcga tctgcgtata ttccgtaatg ttgatataag gattttcttc aaaggtcagc
     6601 tgctccatgg agtacggatg tccgctatcc gcctgctttt cccgcttatc ctgccagtac
     6661 agattctgaa gtaccttgtc cgccagcttc cataccgcct gtgcttccgg gtaagtatca
     6721 tggaattctt ttcccattcc tatgtactga gatccctgtc ccgggaataa atatgcgatc
     6781 ctgctcattt tgtcctctcc taattactat gtgatgctat tgttctcctc ggttacagca
     6841 cattttttat aatgcgccgt tttccatata actacgccgc ctgtcgctgt tcgaaatctg
     6901 ttgtcagcgg ttacctgccg ctatatttta tttatgctgt ccttgtgccg ctatgcactc
     6961 ctgcgcctgc tgcactgttt cttcaataat ctcacggcag gtctgttctc tgcggatcaa
     7021 gcctgcaatc tgtcccgcca tcagtgttcc gtttacggta tctccgtcct gcactgcctt
     7081 cctgagagat cccagcgtca gcttttccag ctccatgaag tctgcatttt ctttttccag
     7141 cttcagatat tctctggtca tctgattgcg cagggaacgc accggatgtc cggtagacat
     7201 tcccgtgacc acggaatcga tatcctttgc cttgatgatc tgattcttat aattttcatg
     7261 gacgatggat tcctttgatg cgacgaaccg ggtgcccatc tggatacctt ccgctcccag
     7321 cataaaagcc gccgccattc ctctgccatc ggcgatacct ccagctgcga tcaccgggat
     7381 agacaccgca tccaccacct gcggaaccag cgccatggtc gtggtggaac cgatatgtcc
     7441 accgctttcc atgccttccg ccaccacagc gtctgctccg gcacgctcca tcatcttcgc
     7501 catagccaca ctggccacca ccggaatgac tttcactccg gcattcttcc acagttccat
     7561 gaattttccc gggtttccag caccagtcgt taccgctttt actccttctt ccacgacgat
     7621 cttcgctaca tcctccgcat tgggattgag cagcatgata ttcacgccga agggtttgtc
     7681 agtcagttct ttgcatttac ggatttcctc ccggacaacc tcaggcggag cagaagcggc
     7741 accgatgatg cctaagcctc cggcatttga taccgctgcc gccagcttat gttctgccac
     7801 ccaggccata ccgccctgga aaacgggata ttctatttgt agaagttccg tgatctttgt
     7861 tttcattgtc tttctgatcc ttctcgtcat tctgcgttat attacatatt ctgatgatac
     7921 cagggtcaaa aggtctaagc ccacatgag
//
LOCUS       DAWDHD010000111         7903 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_538_length_7903_cov_11.972732, whole genome shotgun sequence.
ACCESSION   DAWDHD010000111 DAWDHD010000000
VERSION     DAWDHD010000111.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 7903)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7903)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7903
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_538_length_7903_cov_11.972732"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1966 a         1697 c         2129 g         2111 t
ORIGIN      
        1 agtaagatca aagaacgcag gaggtttaac cctcctgcgt ccccatctca tcggtggtag
       61 agcggatatg ttctactgcc gtttttactt gttctgcata atccagattg ttctggctca
      121 tggtgtgcac ctgctcggca ttggcggtaa cttcttcagt tacagcggac agctgggaga
      181 tgttttccat aatcttatta ttggattctg acaatcccag aatgtggtga tttacttctt
      241 ccacctggga agccagtgct tccatatttt tgttcagctg ttcgaaagtc tcggaagcgg
      301 ccaggatctt ttcgccctgc tgttcggtag cttcaatgga accgttgata gtgttgacta
      361 ccatgttggc attgacattc agctcgttgg taatcttggt gatctcttcg gtaaagctct
      421 tggtctgctc tgcgagctga cggatctgct gtgctacaac ggcgaaaccg cgtccggctt
      481 caccggcacg ggcactttcg atggaagcgt tcagggctaa aagatttgtc tgaccggaga
      541 ttgccatgat gcttccggcg atagtttcca cctcttttgt cttttcctgt aattttgcca
      601 tggcgtcggt gacggaatgg ttggtatcct tgatcatggc ggactgctgt tttaaggatt
      661 ccatagcagc catgttctta cggatgcttt cgttagaatc ttcggcgatc tgcaccatct
      721 gctcggagat atgaccggtc tgctcgatag cattctgaat cgtaccggtc atctggctct
      781 gttcttcgat gctggtggca gtcatattgg tggcagagga gatctcccgc atactgttgg
      841 ctacagattc cgtagtttgt accagctggt tcataagatc tgtggttttg gtggtttcct
      901 gatgtacggt ctgggatacg tccagaacat tatccagtac atcttttaca tggttatgtt
      961 cttcccggga agaaccgatg gcatcattgt taaataatat agtaatctta ccggtttcca
     1021 aaataatgat tccaatcaga aataccagca gggtagagca gacggcattc acatcctgac
     1081 cgtagattcc cttcgtagcc tgaacgatca tgacgatcag gtagagcacg aataatccca
     1141 aagcggtttt cttcagagat tttttctcat aataaggaat ctgtaatatg aaaatggcga
     1201 tcagcgcata gtgaatgaaa cttgcatctg tctgtaaacc gaccaacaaa tactcaatgc
     1261 cgatctcgat ggtagcgaag acttttaata gtcgtgtcgc cttatttcga aggtgtgtca
     1321 cggcatttac tacacagaaa acggcgatca gaatggtatt tgcgtatgta acaatgggtg
     1381 caatgttgtg atttgccagt tttaaccaca gataaaacag gaacacgatc gccaaaagcg
     1441 aagaagcgat aatatagaac cggttcattc tgatattgcg ttccgcggta tccgtataac
     1501 gatatttatt atttcctttt atattcatat tcataagttc cctccctatt tttattgatg
     1561 ctattttaag tgtaactgct ttcgtcaatt ttttctacat ttttattcca tttttgaaaa
     1621 aaattttgaa aaaaatcatg gaaataaaaa atgcaaattg atactgattt tcaataccaa
     1681 atgcgctata atacttctgt tacaacacag tacattaaaa tgttgagata cgaaaggata
     1741 cagaagtgtt atgacactga aagaattgaa aatcggcaaa agcgccgtga tcgaaaccgt
     1801 cggtggttcc ggagaactca gacagcattt cctggatatg ggtatgatcc cgggagcaga
     1861 ggtgacggtg gtaaagctgg ctccgatggg ggatcccatg gaattgcagg tgcacggcta
     1921 tgaacttacg ttacggctgg cggaagcgga tcagatcgag atcgcaccca tatcgaagag
     1981 aacaagagag cataaggggc tggatcgtgt gacggatgcg gagcacccgg gtttaggtga
     2041 ggatggaaaa taccattcga aaaaagacga gaatcctctg ccggagggaa cgacactaac
     2101 gttcgccctg gcgggaaatc agaactgcgg caagactaca ctgttcaatc agctcaccgg
     2161 ctcgaatcag catgtgggaa atttccccgg agttaccgta gaccgtaagg acggttccat
     2221 caaagggtat ccggatactt tggtgacaga cctgcccggt atttattcca tgtcccctta
     2281 cagcagtgaa gagatcgtat cccgtaattt tatcctgtat gagaaaccca gagctatcat
     2341 caatatcgtg gatgcgacga atatcgagcg taatctgtat ctgaccatgc agttgttgga
     2401 aatgaacatt cccatggtcg tagccctgaa catgatggat gaagtcaccg gcaatcacgg
     2461 ctccatcgat gtgaacgcca tggaagcgtt cctgggagta cccgtaatcc ccatttccgc
     2521 tgcgaaaaac gaaggcgtgg atgaactgat ccgtcatgca gtccatgtgg caaaatatca
     2581 ggaacggccg ctgcggcagg atttctgcga taaaaacgat catgacggtt ccgtacaccg
     2641 ctgcatccat gcggtatgcc atctgatcga ggatcatgcg gagactgcga agctgcccct
     2701 gcgttttgca gccaacaaag ccattgaagg agaccacctg atcctggaga agctgcagct
     2761 ggatgaaaat gaaaaagaaa tgctggagca tattgtctgt cagatggagg cggagcgtgg
     2821 cgtggacaga agtgcagcca ttgccgatat gcgttttgat tttatcgaga gattatgtga
     2881 gcagacagta gtaaaaccca aagaaagtaa ggaacggatc cgcagtgaga agattgaccg
     2941 gatcctcacc ggaaaatata cggcaattcc ctgttttatc ctgatcatgg tgctggtatt
     3001 ctatctgact ttcaatgtca tcggagcatg gctccagggt ttgctggaac tgggaatcgg
     3061 aaaattgacg gagatcactg atgcggcatt gaaggcagct catgtcaaca gtgcggtcca
     3121 aagccttgtc atagacggta tctttaccgg cgtgggcagt gtactgagct tcctgcccat
     3181 cgtggtcact ctgtttttct tcctgtctct gatggaggac agcggctata ttgcccgtgt
     3241 ggcgttcgtt atggataagc tgcttcgaaa gatcggtctg tccggacgaa gcattgtgcc
     3301 gatgctgatc gggtttggat gtacggtacc ggcggtcatg gccacgagaa cactgaccag
     3361 tgagagagac cgtaagatga cgatcctgct gacaccgttt atgagctgct ccgcaaaatt
     3421 accgatctat tcgttcttcg tgagcgtatt tttcccggga aaaggcggac tgatcatgtc
     3481 ggcattgtat ctgtttggta tcctgatggg aattctggta gcatttttat accggggcac
     3541 gttattccag ggagaaccgg tgccctttgt aatggaactg ccgaactacc gtctgccggg
     3601 agccaaaaat gtagggcagc tgttgtggga aaaggcaaaa gactttttac agaaagcatt
     3661 cactgtgatc ttcattgcta ccatcgtggt atggttcctg cagagctttg acctgaaact
     3721 gaatcttgtt gaggattcgg caaacagtat gctggctatg gtatccggtc tgctggtacc
     3781 gctgtttcga ccgctcggcc tgggtgactg gcggatctgt acctctctga tcagtggatt
     3841 catggcaaaa gaaagtgtgg tagcaaccct ggaagtattg ttcggcagca gcattgccac
     3901 ggtacttacc ccgctggcag ccgcgtcgtt actggtattc agtctgctgt atactccctg
     3961 cgtggcagcc atcgcttcta tacgcagaga actgggtgga aaatgggcca ttgccgtggt
     4021 actctggcag tgttttgtgg catgggtggc agccttcctg gtacatagta tcggcttgct
     4081 gatgggattc tagaaatttc agatgataag atgacaaata gaaaacctgc aggggattat
     4141 tcctcctgca ggtattttat tacacatttc agttccggag agatccattt gttccggtga
     4201 tacagaagct gccgccagat atccggatga atctctgtca tggggagact gacgagattt
     4261 ccttttgtca gatcttccgc tacaacgaac ttcggcaggt aggagatgcc gagaccctga
     4321 cacagaagat tgcggatcag ctgggtatcg cccacctcca tgataggcat ggtctcccta
     4381 ccgttttggg agagcagatc atccagtgcg gcccggtaac tcatgttttt ctctgtcagg
     4441 atcagcggat attccagaat ctgttccagg gtgacctgag tctgtccggc taaggggtga
     4501 ccggcaccgg tgacgaagtg aatatccacc ggagcttcga aagcggtcac ataattgtga
     4561 ttaaaaatac ggcggtccag cgtgtataca atgtccacct gattctgtcc cagcatctgg
     4621 aacatttcat cggtgctggt agcaatgatc ttcaaccgta ttccgggata tttttcatga
     4681 aaatctttaa aacggctgtg aaattcccag ctggccaggg actctgcaat ggcgatccgc
     4741 accagaccct ccggagcggt attaccgctg atgtgggtgg cttcagcggc gagacgcagg
     4801 atctgctggg ccaggggcag cagcttctcc ccctcattgg tcagggttac ggtatgcccg
     4861 atccgttcaa acagagggat ggacagctcc ttttccagtt gtttgatctg aaaggatacg
     4921 gtggactggg tataacccag acgtccggcc gccagagtaa aactcttcag ttcgttcacc
     4981 ataacaaagg tttcgagatt tttgacatcc atagagtaga tacactttct gtattgtaaa
     5041 ttcaattctt cgatcaatac aatgcaaata atttgttttt caaatattta tgaaaattat
     5101 ataatgagaa aacagggctg tcaatgtgaa aagatggcga aaatgtggga aaacagcaaa
     5161 aatatatgcc gtatcccgtg tttagaataa gtagttttgg gaaggatcag aggtatggaa
     5221 aacagagaga gactggggag tcgtttggga tttatcatgc tgtcggcggg gtgtgccatc
     5281 ggctgcggaa atgtgtggaa attcccgtgg atgtgcgggc agaacggtgg cggaagcttc
     5341 atgctgatct acgtgatctg cctgatcgtg ctgggcctgc ccgccatgat catggaattc
     5401 tccgtggggc gtgcggcgca gacgagtccc ctttatatgt atcggaaatt atccggcggc
     5461 aggggcaaat ggaatctgtg gggcatcgtc tgtctcatcg gaaacattgc gctgatcgct
     5521 ttctatgcgg tggtgtccgg atggatcctc tattattttg taaaatttct gacagggcag
     5581 aatcaggatt tcggttttga aaaaatgatc acgacgccct cggtgaatgt ggcatatctg
     5641 ttagtcacct tgctgatcgc ttttgtcatt ttaagcttca acttgcaggg cggtctggaa
     5701 cgtattacca aatatatgat gtcagcattg atcgtgctga tgctggtgct ggcggtgcac
     5761 agctttacac ttccgggagc aggagaggga ttgagtttct atctgatccc acatttctcc
     5821 aaaattaccg gttctgtggt ggtgggcgcc atgaatcagg catttttcac attgtccgtg
     5881 ggaatgggcg gtatggcgat cttcggaagc tatatcggca aggaccgttc tctcatgggt
     5941 gagtctatcc atattattat cctggataca ctggtggcag tgatcgccgg tatcattata
     6001 tttccggcat gctttactta tgatgtggaa gtgacggcgg gtcccagcct tctgttcgat
     6061 actatggcga ccgtatttaa caacatggca ggcggcagat ggtggggaac cttatttttc
     6121 ctgttcatgg tgttcgcagc catgtccaca gtgctgggtg tgtgtgaaaa tattctggcc
     6181 atggtccggg aactgaccgg atggtccaga ccggcagggt gcgtggcgtg tggtgccgga
     6241 tgtttcctgt tggctctgac gacagcactg ggcttcagtg tatttcattt tcagccgttt
     6301 gcagcaggga cgacctggct cgatttctgg gatttcctgg tgtcgaataa catcatgccc
     6361 ataggttcga tcctgttggc ggtattttgc tgctataagg tcggctgggg ctgggacaaa
     6421 ttcctggagg aagccaacga gggaaaagga ttgaaagtgc agcattggat gaagccggtg
     6481 ttcaagtatt ttgttccggc ggcagtgctg ttcatctata tgtacggaat tgtgaatttt
     6541 gcgtggaaat agagaaaata agacgagaca aacgacggcg gggtaagaac cctgccgttt
     6601 ttgtgtatac gatgaccaag tacagatttg tgcttgcaca agaatctgta tttagagaat
     6661 gtacatcatc gagcttttaa gcgagatggt gtacaaagta tacaaatcga gtaggagcca
     6721 gcctgctcga ttgtatagga tgagatggga agcggttgca ccgtaactca aaattgggta
     6781 gtatgtaact attcagaggc atgtaaatca ggtcaatatg tacgtcgagt gcttgcactc
     6841 gcaagaacat ttgacatgat taatagggcg aatgtgattc tgaataatta tgtggtatgc
     6901 tatgtagtat aaagatatga aagcaggaga catctatgac agagcaggag atccggacga
     6961 tattgaacag acaaagagaa tattttgcca cgggagccac actttcccgg aaatttcggg
     7021 cagaacagct gggaaaatta aaggcctccc tgcagcgcca tgaagaggaa ctgaatgctg
     7081 cactgcaaca ggatctggga aaaagccgga tggaaagcta catgtgcgag attggactga
     7141 cgctctcgga gctgacctgg atgcagaaac atctgcgggg gctgatgcag gaaaagagag
     7201 tgccgacacc gctgtcacag ttcgcagcga gaagctttcg ctctccgtca ccctatggca
     7261 ccgtgctgat catgagcccc tggaattacc cggtgctatt gacactggat cctttgatcg
     7321 atgctattgc cgcagggaat gccgcagtgg taaaacccag tgcctatgct ccggcgacgg
     7381 cagcagtgtt aaaaatgatt ttggaagaat gcttccaggc ggaatatgtg acggtgatta
     7441 ccggtggcag agcagagaat caggcattat tgcagcagcg gtttgatatg atatttttca
     7501 ccggaggcaa gatcgtggga cgggaggtgc tgagaagtgc cgcggaatac ttgaccccgg
     7561 tgacgctgga actgggtggg aaaagcccct gcatcgtgga tgctacggca aagatcccgc
     7621 tggcggcccg gcggatcgtg ttcggaaaat atctgaattg cgggcagacc tgcgtggcac
     7681 cggattatat tttgtgcgat ggccagatcc gggatcagct ggtggaagcc attcaggctg
     7741 aaatcatccg acagtttggt gcggatcccc tgcaaaatcc ggattatgga aaaatcatcg
     7801 acgatatgca ttttcacaga ctgctggggc tgatggatgc ggtaatgacc gtctgcggcg
     7861 ggcagtttga cttaatgagt ttgctgattg ctcgcacagt gtt
//
LOCUS       DAWDHD010000112         7896 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_540_length_7896_cov_13.622370, whole genome shotgun sequence.
ACCESSION   DAWDHD010000112 DAWDHD010000000
VERSION     DAWDHD010000112.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 7896)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7896)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7896
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_540_length_7896_cov_13.622370"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2423 a         1527 c         2020 g         1926 t
ORIGIN      
        1 ttgcctgaca tggatggatt cccgagtggc caaaggggac agactgtaaa tctgctgcaa
       61 tttgcttcgg tggttcgaat ccacctccat ccattcgcac gttgcggttt gcgcgttgcg
      121 atgcgtccgc tggtgtggcg gaataggcag acgcaaggga cttaaaatcc ctcgggggca
      181 accccgtgcc ggttcaagtc cggccaccag catacttaat tagtgagagg aacacggtag
      241 aaatgccgtg tttctcttat tttttattgt gaatgataaa aagaaggaat ataattgtat
      301 acaaataaca gtaatatatt ctcaagacga aggggtgaca gtatgctgac aaatacaatg
      361 aaagaggatt tgacccaagg ggtagtagat cttttttcgg atagtatttt ggcaatcata
      421 ttgtacggat ctgtcgccag aaatgataat acggatgaga gtgatattga tattgtaatt
      481 atcataaaaa atgaaatgga tgatgcaaca aaggaacagt tcattcattg gtcagcggaa
      541 ttagatcttc gatatgacag agtattttct atcattgata ttcaggaaga aaacatggag
      601 aaatggggaa atgttttgcc gttttatcag aatatataga aggaggggat aattctttgg
      661 aaagcagaat aacaattcct tttacacttc atggaataga agaaaatctc accgtagaat
      721 acacgcaagg tacagatccc agggaaattg gctatgatgc ccttcaggga tttccgatag
      781 acaaaatatg ttgcataggt tatccagtaa tgcatggtta ctttgaacat atgaatgcga
      841 ccggttatcg gagatcctgt ggatttattc aattcgtgga aagaattgaa aattatgggg
      901 aaaacagaga gctttccata gatgtcagtg atgaaaatct gggaaaaggg aatccatatt
      961 ttgcgtatgg ttatccggca gaaatatttg atgctccctg ttgtaatttg gctgggtgta
     1021 gacatttgaa atggacagct tatacctatc tagtagatct gccctcccgc atgaatagca
     1081 ataaactgaa attcctgggc ggatactcct ggggatatga agaggatgaa aacggtccac
     1141 agagattatt accgctagag atattgacag aaaatgactg ggaaaagcat gcaacggaaa
     1201 aaggaatatt aaagatatcc caaataacag gaggctgtac tgctaagtta tgaagaatag
     1261 atataaacaa aaactgatct tcgttattgc aattttatgt ttgagcttag taagctgtaa
     1321 aagtaaaaca gagccaatta ataatattgc atcatggacg ccagatgatg gttggacaat
     1381 aaatgaaatt gatattaggg atctgtggcg aatgtggaat gggagacaac agtatgaatt
     1441 attatatcag tgatctgcat ctatttcatg aagcatccat aggattcgat ggacgcccgt
     1501 tccggaatct ggaggaaatg cacgcagaga tcgtaagaaa atggaacagc aaagtaacta
     1561 acggagatac cgtatacatt ctgggtgata tgagcatgcg cggtaagaac gaggatctga
     1621 tcgctctggt ggcaatcctg aagggaaaaa aggtcctgat ccggggcaat catgatgatg
     1681 tgtccgatct tagataccgg cagttgttcc aggaagtctg cgattatagg gagatccggg
     1741 attctgtagg cggtaagagt tatgaactgg tgctgtgcca ttaccctatt ttcagctgga
     1801 agcatatgag ccgcggcacg attctactct acgggcatac ccacaacagc ccggaagata
     1861 tgtattttca gaaatgtctg gaaggaatga aagacaacga atgccgacat accggtgaca
     1921 gtcacgtaca ggcatataat gtgggctgta tgaagccgtg gatggactat gaaccgagaa
     1981 gtctgcagga aattataaaa taaaaagctt tgaaagaaaa atcgagcagg ggaaccggct
     2041 cgattttttg caaccctaga tttgatgaaa gatctgaaaa aaatatgtgc tatgatggca
     2101 gtgcaacatg aatcacagac cattaggaaa ggatgaagaa acagatgcag ttacgtgaaa
     2161 tgatagacaa gtatccgcaa cagtatatcg cggtggccta tacgaaaaaa ggtatcgaca
     2221 acctgatcga gacagcaacg gttttaaaag tgtacccgac cttgctggat gcctatgata
     2281 atctgtcgga gatcaaagca tacaaaaaga gatactccga ttttgacatt gtatacggag
     2341 actatgaaga ctatgtatcc acaaggcgca aagttgtgat gacaaagacg gatgaaagac
     2401 ttacccagga ggaaatcgac aaattgctgg caatgataga tcactaaagg aataccgatg
     2461 atctgcaggc ggagattgcc acagaaaaat ctgcaatatg catttttatg cgcgattatg
     2521 tcagaggcga aaagggaaaa tgacaatatt tgattatctg aaaaagaatt gtgaagtggc
     2581 gatatatacg gatgaatacg ggaataccta tatggagacg aaggaatggg agtatgaaaa
     2641 aattatttcc ggagccatag aaatcagcaa taaagcagac gatgctattg tctggctgat
     2701 accgaaagaa gtttatgaaa agcattcgga aattgagatc gctattgccg gggatgagtc
     2761 agtcgatccc gttcgaaatg tccgcaggcc ctattaccgg atgagaggag tacccgtaac
     2821 ggcagagcag gcatttgata ttatccgaag gacggacaga ttttaaaatt tttacgtgag
     2881 tgctgttcgc agccatgaag attatatcgg atgtatgaat tttgagaact gtctcattca
     2941 gaagaatcat tatcccacgg gatatggctg gattcgtgcg gatggaacga ttggggcgaa
     3001 tgcgactacg caaaaatacc ccacggtcag ggaatttatc gaagaatggt ataaactgct
     3061 ctatgcattt ccttatctga atctgatcat tgcggttacg gggtggaacg aaggaccctg
     3121 gggtgacgaa acggtatcgg aggaggaatt ttgcaaagag gttgccgtgg ggatatatgt
     3181 ccatgacagg aaaatagaga tcctgaatcc gccaaatacc atagcaaagt ataaggaata
     3241 taataaacgc tacggaacac cgcctgagaa attcgagcgg gagtattacg agaagcataa
     3301 gtacgagagg tataagaccg aacaggcgaa tccggcgtat ttgagaaagt gcatcgaagc
     3361 ctatgggctg gatgcggata aaatcttgaa aaggggataa gaaaaatgaa taatccattt
     3421 attgatgtat tttgtccaca ttgtggagaa tttgtgaacc caatctatga aggggactgt
     3481 cctaattgcg gagaatatat aggatctgac gacaatgagg aatgggatga cgggagtgat
     3541 ggagacggat attattttca tacctgaaca aaaaaacagc agatcccgcc agatctgctg
     3601 ctttttctgt gtatatgagc gtgaaaaaca cacttctatc agaatttttc agcgaagctc
     3661 ttgtaatact cgtcaaaaaa ctcgttaacc ttttccatga tcttcttcat aatattcacc
     3721 cttaaccttt ccaagacaaa tgaactgttg actttgttgt tacgcttaag gggtcaggcc
     3781 tgacttcctt ttgatgatta taatttacca tattgcgctt ataatgtgaa atacatataa
     3841 tagaagtata ataataccta aaaggtataa taaaagaaaa tacaacatgt tacaaaatag
     3901 aaagattaag ctggtttttg gaaaaagcat ggaaggagga tgctggaaag atggaattgc
     3961 ggcatatccg gtatttcaag gcagtggcgg aggagaaaag cttcacgaaa gccgcggagc
     4021 agctggcgat cgcgcagccg cccctgagcc gtcagatcca ggatctggag gcggagctgg
     4081 ggacgacatt gttccttcgc acaccccata aagtgtccct gacagaagag ggagagctgt
     4141 tcctgcagta tgcgaatcag atcctggacc tggtgaaccg gtcggaggag gatgtcagag
     4201 aacgcaagga aggactgcag gggacattat acattgcgtc ggtagagggc tgcggaccca
     4261 gacttttcgc ggagtggatc gctggatttc agcagagaca tcctcatgta cagtacaatc
     4321 tgtggaacgg caacacagat gatgtgaata accgcgtaac gaagggattg tgtgagattg
     4381 ccatgatcac ggccccttat aacacagagg aattccacgt gctgccggtg tatgaagaac
     4441 cctgggtggc agtgatcccc aagggacatg cattgtattc ctgggaaaat gatccggtga
     4501 agccctcgga gttgttgccc tatgatctgt tgattccctc cagagaatcc agaaaaggtg
     4561 aaatcgataa atggttcgat ggcacagggc atgctccggt gatccgggga aggatcgcac
     4621 atatgatgaa tgcctacgaa ctgagcctcc acggcgtcgg catctcgatc tatccggcat
     4681 cgatttcctc cctgatccga gacaaggacg tctgcgtgcg gccggtggag catccgaatg
     4741 cccatgcgga ttatgctttg atctggaata aaaatcatac cctgtcccat gtggcggaag
     4801 aatttattgc ttatgtaaaa gaagaatccg gacagcaggt gtactatgcc tgaattaacg
     4861 caaccgatac gtcattcaca tttgtgtcgg tagctcctgc cttgtcaaaa gaaaggaaag
     4921 cccttatact tggaagcagg aggaaaatac gatgaacatt acagtatatc tgggagcaaa
     4981 tgaaggaaat gatccttctc ttagaaaagc ggtgcgtgag ctgggaacct ggatcggtga
     5041 aagcggtaac cgcctggtgt acggtggatc caaatccggt cttatgggag agatcgcgga
     5101 gagcgtatta caggcaggcg gcgaggtgac cggcgtggaa ccgcagtttt tcattgatat
     5161 ggaataccag tatgatgcca ttacggagct gatcgtcaca aaggacatga cagaacgcaa
     5221 gacgaaaatg atcgaactgg gagatgtatt tattgctttt cccggcggaa ccggaacact
     5281 ggaagagatc gctgaagtga tgtccaaagt gtcattaaag cacctggagg caccttgcat
     5341 tctctataac cttaacggct attatgacag cctgaaagca ctgttatcac atatgacgga
     5401 gatgggattg tcctccccgg agagacagga aggcatttat tttgccaggg atctgtcgga
     5461 aatccgcaag atcatagaaa atcagcggag caatgtaacg atcgctccgc cggagaatac
     5521 acaaggtatg ggaaatcact taggttaaca ggttgaagcc cattactacc agacccagga
     5581 tcacgagaat cacaccggtg acacggttca gagtcttggg tgtggccttg ttggcgaaca
     5641 gtgcggcaat ccgggcccag atcagggtaa agatgacgca caggatccat acggtccagt
     5701 cgggcatgcc cccaatggca aaatgggaaa ctgcaccggt cagagcggta aaagccataa
     5761 taaagacgct ggttcccaca gcagttttca gctcatagcc cagaacagaa gtcaggatca
     5821 acagcatcat catgccgccg ccggcaccga cgaaaccgca gataaagccg atcagcatac
     5881 cgcataccag agactgaatg gctctttttt tcgcggagac gccctgcatg gcttctttgg
     5941 tggtcattac gggccggacg atgaatttga tacctaacag gaaagtcatg aacacggaga
     6001 aattccccat ggtagcggaa ggcaccaggc tggatacgta gctgcctacg acggtgaata
     6061 ccagcacact tcccatcatg atcaggccgt ttttgacatc aatattttta tttttcgcat
     6121 aggtataagc ggatacggca ctggccagaa cgtccgagga cagcgcaatg ccgacggcca
     6181 gatacggatc catgccgagg aaagtgatca gcatgggact gatgacggca gcagcgctca
     6241 ttccggcaaa tccggtgccc aggccggctc ccatgccggc gaaaaaagtg acaagaatcg
     6301 tgattaaaat ctgcatttat ttatctcctt tcaggatatt gtccatattt ctctccatga
     6361 catgaagcat gtggaaaaag atctctttgc ttgtctcatc ggtgccatct agcagagtat
     6421 ctttgaaaat gctctgtatc atgcggcctt tttcgatgac atcctgtgct ttctccgtgc
     6481 agaacagcag agtcttccga cggtcatccg gagcctctct gcgttccagg tacccctcgc
     6541 acaccagctt gtccacattg acggacacca gattcgcctt gatccggcgg atctccacga
     6601 catcactggc agtgtggtat tccggattat tgccgaagaa catgagaata tcaaaagctg
     6661 tctgtggcag ctttaactgc tggcacagag gtttgcacat ggcgttgtaa gccagattga
     6721 atttttgtac gaattcgata tcagtattca tccgaaggcc tccttactta gtattataca
     6781 aagtgtaatt actgaaatta caaaagtgaa tatttcaaat ttgaaataat tgctatattg
     6841 catagtatta cagatagttt tcgaagtcaa tcataagata ttttatttca taaaaaattt
     6901 atattcatta tactatcgta aacatcattt cgatatgcta taatttattt gatgcaaggg
     6961 tgcattcaga gactgtgggc atacagaaga aagtgtcggg aggagacatg agtatcaata
     7021 catatgatga acagaaattc tgggaggaaa tggatatcct gttcccggat attgtaaaag
     7081 ccattacttc tctggcgaaa aagtcctaca tatccataac caatctgcgg aacggcgtga
     7141 catggtggtc cgagaagacg atggaatatt ttggtatgca ggagaactat accatccgcg
     7201 gacaagaaaa atcaaaaaga tcgattcatc cggatgatct ggaggatttc cggaggggat
     7261 tccaggagag agttgccggg aaaaatatgg acgagccctg ggagtaccgg gtgcgtgacg
     7321 gaagcaccta caaccggatc agcgccaggg cgaaaatgct gaatgataag gatggaaagc
     7381 cttttgtgat cgtaatacga tataacaact acggtatttc cgacgaggtg gacgcgacca
     7441 ccggattgca tacggaacct gcgctggacc gggagatcag ggaattcctg gaggaaagcg
     7501 gacaggggac tctgctgaag atcggactgg atcagttcag tcatatcaat gttatgtacg
     7561 gtgccgcttt ttcggataag attctgaact gtgcggcaca ggagctgttg cgcctgttaa
     7621 ggggaaaagg atatgtatac cggctctccg gtgcgaaatt tgtgatcagc tttaaaaatg
     7681 tatccaaaga ggaactgcag aagatatacg atgagatcgt agaagctttt gaaaatacag
     7741 aggtagaggg gaaaaagatc cctctgaagg tatctgccgg cgcaatattt atggaacctt
     7801 atatgaagga gacgaacgcg gtccgcagcc gtctgactta tgcgttgaac cattcccggc
     7861 ttgagcatca tggagagctg gtgattttta atgatg
//
LOCUS       DAWDHD010000113         7815 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_546_length_7815_cov_8.799098, whole genome shotgun sequence.
ACCESSION   DAWDHD010000113 DAWDHD010000000
VERSION     DAWDHD010000113.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 7815)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7815)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7815
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_546_length_7815_cov_8.799098"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2246 a         1584 c         2192 g         1793 t
ORIGIN      
        1 gctacgaaag agaccaccag cattaccggt gtcttgtatc agtattcttt tatgttgatg
       61 ctgatcgctt ccggaatgta caggtatctt ttcggagcac tggcagacag ctttacactg
      121 atcccggtga acggggtggt gtttcacgcg gacagtctgt tacaatccat gacagaattt
      181 atgagtgatt atatcctgat cggctttcgt attgtattgc cgattttttg tgtgatattg
      241 cttctgaatg caatcctggg agtgctggcc aaggtgtctc cccagctgaa tatgtttgca
      301 gtaggtattc agctgaaggt tctggcggga ctgacggcaa tgttccttac caccgggatg
      361 cttccggggg ctgccgactt tgtctatcag gaaatgaaaa agatggtagt atcttttatc
      421 ggaggtatga tgtgatcctg gaatatgatc tgcaattctt cgctgcagaa gggatgggcg
      481 gagagaagac agagcctgct accgagaaaa agctgtctga tgccagaaaa gaaggtcagg
      541 ttgccaaaag ccgtgaaatt gccaacgggc tgggtctgct gacggtattt ctggtattga
      601 aaatatgggt aggacacatg gggtatcagt ttataaatgt gttcagcgtc atttacgaga
      661 gaattcccga ggtggtgact ttctggcatg gaaatatgcc acagcaggac accgcaatcg
      721 cattccggca gatgctttta caggccatca tcattatggc accgctcctt ttgctgggat
      781 tagtggtggc atttttgagc gatgtggtgc aggtgggctg gaagcctacg ggaaaaccgc
      841 tacagcccaa gttcagtaaa ttgaacccgc tttcgggctt caaaagaatt ttttctgcca
      901 atgcgctggt ggaactgatc aagtccattc tgaagattgg attgatcact tacatatgtt
      961 ataattatct gaaagataaa tggcctctgc tgttttccct gtatgatatg gatctgatgc
     1021 aggctattgc attgatcggc ggaacggtga cagatctggg aatccgggtg tctctggtct
     1081 atatgatcat ttcggctgcg gattttatct accagaagta caaattcagc aaagacatgc
     1141 gaatgaccaa gcaggagatc aaggaagagt acaagcagca ggaaggtgat ccgcagatca
     1201 aggggcgtat ccgtcagaag atgcaggaag cttccagacg gagaatgatg cagaatctgc
     1261 cccaggcgga cgtggtcatc acgaacccca cccattatgc ggtggcaatc aaatacgatc
     1321 cggaggtggc ggatgcccct attgtgatcg caaaaggtga ggattatctg gcagcgaaga
     1381 tcaaggagat cgccagagag catcagatag aaatcgtaga aaacaaacca cttgccagaa
     1441 tgctatatgc aaatgtggat gtgggacagg cggtgccacc ggaattgtac caggcggtag
     1501 cagaagtcct ggcgtttgta tatcatctgc aagggaaagt atagaagtat agagaagaaa
     1561 tagaaacatt agcaaaaaga tgaaaataca caaaaagaga aaggaggtca ggcacagatg
     1621 aaaatgaaaa aagcggatgt ggcggtagca atttatatca tggctgccat tatcatgttt
     1681 attgtgccga ttccctcctg gctgctggat attctgttag caattaatat ttccgtggcg
     1741 ctgacaatcc tgttcgccac gatgttcagt aaggaagttc tggatatgtc cttcttccct
     1801 acgatgctgc tgtttaccac gatcttccgt attgcgctga atacctcatc cacccgtctg
     1861 atcctaagag acggcaatgc aggtaatgtc gtagaaactt tcggtgagtt cgtaggtggc
     1921 ggtgatctgg tcatcggtgt cattatcttc atcattctga tcctgatcca gtttatggtc
     1981 atcaacaaag gttcagagcg tgtatctgaa gtaacagcga gatttaccct ggatgcaatg
     2041 cccggtaagc agatggctat tgatgcggat ctgaacaccg gagccattac agatgcggaa
     2101 gcaaagaaga gaagagagaa aatccaggaa gaggcaagct ttttcggttc catggatggt
     2161 gccgtaaaat atgtaaaagg agatgcggtt gcgggtcttc tgatcaccac catcaatatt
     2221 gtgggtggta tcatcatggg aatgacgaga caaggcatgg atattaccag cgctctgaat
     2281 aaatacgcta tcctgactat cggtgacggt ctggtaagcc agatcccctc cctgttgatc
     2341 tcactgtcca ccggtattct ggtgaccaag ggaagcaagg atgctgactt cggtacgaca
     2401 ctggtaagcc agttgttcgg tgtacccaag ggtctgtacc tggtgggagc catgctggca
     2461 atcctgggat tcgtgacaga attgaacacg atcctgttcg tgggattggg actggtgttt
     2521 atcatcgtgg caagaaatat cgagggcacg atcgagacgg cacagatcga acaggaggta
     2581 gacagcgagg aagctgcggc ggaggagatc cggcagccgg aaaatgtcaa cagtctgttg
     2641 caggtggatc ccatcgagct ggaatttggt tacggtatta ttcctctggc ggatgtgaac
     2701 cagggcggtg acctgttgga tcgtgtggtt atgatccgaa gacagatcgc gctggaactt
     2761 ggtacggtgg tgcctattat ccgtttgcgt gataatattc agctgaatcc gaatcagtat
     2821 atcatcaaga tcaaaggtat tcaggtcagt gagggagaaa tcctgtttga ccattatatg
     2881 gcaatgaatc ccggttatgt ggaagaggag attacgggta tccccacgtt cgagccttcc
     2941 ttccatctgc cggctatctg gatcacggaa ggtcagagag agcgggcgga gagcctgggc
     3001 tacacggtag tagatccccc ttccatcatc gctacgcatc tgacggagat catccgccag
     3061 cacattgcgg agctgctgac cagacaggat gtacagaacc ttatcaacaa tgtaaaagag
     3121 aacaatcctt ctctggtgga cgagctggta ccaaagttgt tgggactggg cgagatccag
     3181 aaagtattgc agaatctgtt gcgggaggga atctccatcc gggatctgct gacgattctg
     3241 gagactctgg cggattatgc accgacgaca agggataccg atatcctgac ggagtatgtg
     3301 agacagagct taaagagagc aatctccacc aagtatttcc cttcccacga gactaccagt
     3361 gtgctgacgc tgtatccata gctcgagcag gaagtcatgg gaagcgtaaa acagacggag
     3421 acgggagctt tcctgaatct ggatcccgcc agaagcaaag caattctcaa tgcggtggga
     3481 gaagagatca aaaagctgga aaacatggga aagaccccca ttatcatgac atcaccgatc
     3541 gtaagaatgt atttcaagcg gctgaccgaa gattattatc cggatctggt cgtagtgtct
     3601 tacaacgaag tagaatcgaa tgtggaattg cagtctgtgg gtatggtcac agcttaagag
     3661 attaagcgca ttgaaccgac acaggaacga ggaacagtat gataattaag aaatttaccg
     3721 ggaaaacaga agcagaggca acagaggctg cgaaaaaaga attggtatac ggactggtga
     3781 tcatgaatgt ccgtgaagtg aaaaagaaag ggctgttctc ttttctgtca ccgaagcaga
     3841 tcgaggtgac cgctgcgctg gaggatgagt ccccacagag atctgtggta aacagtctga
     3901 atcgtacgga agcttccggg gcaggtgtag gtacggcgaa gactgtatcg atgaaaaaca
     3961 gcacggaaaa tatagaaaaa aagctggata gtctgcagac gcttttggaa agtcagttaa
     4021 acagcagacc ggtagatgca aatacggaga aggcagcgga agcacagaaa gaagctttgg
     4081 acagtgaagc ggaagcagca gtggaaaagc aggaagaaaa agaggacgag cagaatccgg
     4141 agcaggataa gttcatccgt ctgctgtata acaaaatgct ggataatgag atggatgaaa
     4201 aatatgtcaa cagtattctg gaggatgctt ccaagacaaa gaaagcggat cttccctttg
     4261 attatctact ggcgaatatt tatcagaaga tggtgttgaa attcggaaaa tccgaaggaa
     4321 tcactccggc ggaggacgga ccgagaatcg tatggttcat cggacctacc ggtgtgggta
     4381 agacgactac catcgccaaa ctggcaggaa aatattgtgt ggaagacaag aaaaaggtgg
     4441 ctctccttac ggcagatacc tatcgtattg cggcagcgga gcagttgcgg acctatgcca
     4501 atattctgga gacacctttc cgcgtgatct atacaccgga ggagttgcag accgccattg
     4561 atgattactg ggactgtgat tatattttta tcgatacagc ggggagaccg caccagaatg
     4621 cggaacagtt ggagaaaatg aaggaaatgg tgggtggact gaagagaccg gaggactatc
     4681 aggtatttct ggtattaagt gctaccacaa aatacaggga tctccagaag atcgcagact
     4741 gttacggaaa gatcgcaaag tttgagttga tttttacaaa gttggatgaa acagaagcag
     4801 tggggaatct gctgaacata aagctttata cggatgcacc gatcgcatat gtgacctgtg
     4861 gtcaaaacgt tccggatgac atggaaatgt tcaatcctca gaagacggtg aaacagatcc
     4921 tcggaggtaa ggaatagaga ggaaggaaga aagctttaac agaaaggcgc gtgaaaatgg
     4981 atcaggcgga acagctaagg atcattaaag caaataacca aatacgacct actgcaagag
     5041 tcattaccgt tacttccggc aagggtggcg tgggaaaatc caatatgtcc atcaatctgg
     5101 caattcaatt ccgcaaaatg ggcaaacggg tgatcattct ggatgctgat ttcgggctgg
     5161 cgaacatcga gatcatgttc ggagcaatac cgaagcacaa cctgtgtgat ctggtttacg
     5221 agggcaagag catcagtgat atcattacct ggggccctat ggaagtggga tttatttctg
     5281 gtggttcggg aattgccggg atgtccaatc tgggaaaaga tgccctgacc tgtatcatac
     5341 agaatctggc ggagctggat gccctgacgg atatcattat cgtggatacc ggagcgggaa
     5401 tctctgacag tgtgttggaa tttctggtgg ccagcgggga agtgctgctg gtgaccacac
     5461 cggagcctac ttccatcacg gattcctatt ctctgctgaa ggcactgtac agacatccga
     5521 gattccagga ggatttcacg aaggtgatga tggtggccaa ccgggtaagt aatatcaagg
     5581 aaggccaggt actgtttgac aagctgaatg cagtcgtgac cagatacctg aaaattccca
     5641 tgacttatat gggatgtgta cctcaggatc ctcaggtcat gcaggcggtg atgcagcagg
     5701 tacccgtatc catacagaat ccgggagcaa aggcaagcca ggcttatcag agaatcgcag
     5761 agagaatgtg cgaaaaagaa gacgatacag cgcaggagca gcagcccagg cgcggaatgg
     5821 cagcattttt ttctcacatt ataggtacga gaaatggagc tacaaaacag tccagataag
     5881 agaggtatga acttatgtta tccagatttg tggaagaagg atcgaaaata gagctccgcg
     5941 cattgcagcg ggggttggag gaaaagggag cagaaagcac ggcaaaggtg taccagagcc
     6001 gggtgctgca gattctgtcg gaggataccc tggagatctc catgcccatg gagcagacca
     6061 gactgatact tttgccggtg gactcggaat acgacatgat ctttttcgag gagaacagcc
     6121 tgtaccagtg ctttgcaagg atcatagacc ggtacaaaag caacaacgta tatgtgctgg
     6181 tcatggaact gaccagcaat ctgcggaaat atcagagaag agaatattat cgtttcagct
     6241 gcgcactgga tatgtgtgcc agaaatctgg aggaagaaga gatccaggca ctggagaaaa
     6301 attctcctta tgaactgcag ccgggacttc ctttgaaaca cagcgtgatc gtggacatca
     6361 gcggcggagg actgcgattt gtgtctaccc agcgatatga acccggcagt ctgatcctgt
     6421 gcagttatca tctggtgaaa gacggagaac agaaaaagta cgaggtggta ggcaaagtac
     6481 tggcggtaag agaactggag aaacgcaagg ggacgttcga acaccgggta cagtactata
     6541 acctggacgt aaatacaaga gaagaaatca tccgctttat ttttgaagaa gagcgtaaga
     6601 gtcgtaaaaa ggagagattg gactaatgcc aaaaaaaata ctggttgttg atgattctgc
     6661 actcatgaga agagtgctgt gtgatataat agatactgac ataaggttcc aggtggctga
     6721 ccgggcaaaa gacggattgg aagcattcga tctgttgagc cgaaacagtt acgacgcagt
     6781 tgtcctggat gtgaatatgc cgcgtatgaa cggcctgcag cttttacagg agctgcgtaa
     6841 atacaagatt cccgcccgtg tgatgatggc cagcaccgat acgaaggaag gcgccagaac
     6901 cactctggat gctctggaac tgggagcgtt ggattttgta cataagccgg acagcgcagt
     6961 agactgtcgt ggtgagaatt tccgcagaga attcctgcgg acactgaatg tggtagcgaa
     7021 cagcaaactg ccggtatttg ccgaggaagc gaagcttacc gaggaaaaag gcacggtaaa
     7081 agagatgatg aagatcatca atcatcatcc ggtcagcgtg tcgggtagca aggtcgtagc
     7141 cattgccagt tccacaggag gcccgaaatc tctccagtcg gtgattccct tattgccggc
     7201 gaatctggat gcaccggtgc tggtagtaca gcatatgcct gtgggattca cagcatccct
     7261 tgcagaacgg ctggataatt tgagccagct tcatgtaaag gaagcagcag agggagaaga
     7321 actgaaaaag ggatgggtct acatcgccat gggcggcaaa caccttaatg ttgtcacatc
     7381 cccggctgga cgccatacgc tgcatctgtc ggaggaacct tacagggaag gtgtcagacc
     7441 ctgtgccaat tatatgtatg agtccctgca gaccagccgc tttgattcgg tagtctgtgc
     7501 cgttatgacg ggaatgggag cggacggaac ggaaggaatc gggaaactga aagcttccaa
     7561 aaagtcctat gtgatcgcac aggatcagga taccagcgta gtatttggaa tgcctaagag
     7621 tatcatcgca gccgggctgg cagatcaggt agtaccgtta gatcagatcg cccaggagat
     7681 catactgcat gtaggagtag taaagtagca agcaatatat gccgtttccg gcaataccgt
     7741 gaccggcaga atggaggaag agatggacgt aagccaatat cttgaaattt ttctggatga
     7801 aacaaatgaa catct
//
LOCUS       DAWDHD010000114         7753 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_550_length_7753_cov_10.771629, whole genome shotgun sequence.
ACCESSION   DAWDHD010000114 DAWDHD010000000
VERSION     DAWDHD010000114.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 7753)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7753)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7753
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_550_length_7753_cov_10.771629"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1831 a         2126 c         1700 g         2096 t
ORIGIN      
        1 ctgacaaaag tgcttccggc atttcggttc cgtcacagtc tcttaaagcg tctgcaccca
       61 gaagattttt taagcgtatc gtttcgtcaa tgatgtctgc atcggtcggc agcgtcagtc
      121 ttcctgtcat cgcatggttt cctcccatca cttttgtact atcttgcagc tgatgactaa
      181 agtatatcgg cttcctgacc tgatatcaat ttctttgttg cttgttttgt tatgtttatt
      241 gctattttgt tactaaaata gtggattttt tgaaatgatc atgtataata tgcttatccc
      301 aatcatacag atttttattt tttcgtcttt tttacgaatg tttttgtaaa atattgttct
      361 ttttatatat tcattataaa aaatactgtg aatttgaaaa agtgcacaat aaaataagat
      421 aaagaaggta tccatttatg agtaatgaat ttatcaatat cagatcggca aacagttccc
      481 cggaacattt ccagcttcgt tatatctcca tctccaaata cgaaggtgac tggcagtccc
      541 ttccgcatac ccaccacttt tcagaactgt tctatgtcct gcggggtgag ggagccttct
      601 atatagagaa cgaaaaggtg cctgtgaaga cggacgatct ggtcatcatc aatccctatg
      661 tagagcacac ggaaaaaaca cttcccaacg atcccatgga atatatcgta ttcggtgtgg
      721 acggacttgc attttccttt tccggtgctg ggcaggacaa cccgaagggc tacagcttct
      781 acagctacgg ctctgacaaa aaacagttca tcaacttcgc ccagctgatg atgcaggaat
      841 tccgcaacaa gcttcccggc tttgaacagg tatgtcacgg actgctccag gtgctcctgg
      901 tatatatttc ccggaagcag aacctgtccg tcatctccga ctcctccttc caattatcca
      961 aggaatgtgc tctggcgaag cggtatatcg actccaatta ttcccagaac atcactttgg
     1021 attccctggc ggagatcacg catatcaaca aattctatct ggcacactcc tttaccgaat
     1081 gcgtcgggca gtctccgatc aattacctga cagaccggag gctgcaagcc tgcaaggagc
     1141 ttctggtatc ctccaatctg tcggtagctc aggtagccac cagcgtcggc ttttcttcgc
     1201 agtcttattt ctcgcagatc ttccgtaaaa agaccggcat gacaccgcgc cagtaccgga
     1261 accggtatgc cagaaaataa tgtgtacact cataaaagac ctcactgccg gcgcatctgc
     1321 cgactgtgcg gtcttttata atggacattt attcacttat ttgatccagc cccagcataa
     1381 tcccagatat tccaccagaa aatacatccc ccgcagccat atcagcacgg cacagccata
     1441 gaacatccag acattttctt tctgcctgtc tgcgattttc cctgccatgg ataaaatcgc
     1501 acaaatcaac aaaaccatcg ttccgaaagc cgcccgatcc ggatagtgtg gggacaggat
     1561 catggcaccc caggacaaca gtgcccccag caataacagc actgtatttc tgcccaggtt
     1621 ttctttcaga atgcctttgc agacgatcag tgcaaatacc gtaagcagca gggcagggaa
     1681 cagatactcc ataactccct tcgcttctgc gtagcagcgc agatacattc tccacagcag
     1741 gctgtaggct tcttcccccg tctccccgct gcggacaaaa ttgcccggtg cagtgatcat
     1801 cagaatggtt cccaccaggc agctgatgtt tcccagatac atccacaccg gagctttttt
     1861 gtgctctctg tgtcttaaca ggatcaccag aagactcagg agccataccg caggccccat
     1921 attctcattg ctccagcctg cgatcagtcc cagtggaagg atccagataa cgatccccgg
     1981 cagatttttc tcctcatatt tcagatagca gaacaaaaat gccagaagaa atcctgtcat
     2041 gtacagataa ttcgctgcac ctgcttccca gaacatgctc attttccagt tggcattcag
     2101 ccccaggatc atccccagcg ctgcaaacca gtacgggagc ttacgtctgt ctgctatcat
     2161 gcaggccagc catcccaaca gcagtgtcat tcctgtattc aggatatccg cccagtgttc
     2221 tccggccagc aggatcagct gtaacaggcc atgagccata cttctgccgc cccagttgaa
     2281 ataatgccag atctgggcat ggataatgtc actgagattc cggatcggtg tatccgcata
     2341 caataccgtc gaataccact catcatccat cataaattcg atctcatagt tgacgcgcca
     2401 cacgatcagt gctgtcaatg cgatcagcac cgggctgatc caccggattg cttttttctg
     2461 cttttcctgc atatatctgc tccattcctc ttatggcaaa gaacggctgc ctataactga
     2521 atcttcttca gacgatccac tgcctccatg gtattctcat agcttccgaa agccgtcaga
     2581 cggaaataat gctctccgct gggtccgaaa ccggatccgg gcgtacccac cacgttgaca
     2641 ttctccagaa ggtaatcgaa gaactcccag ctggtcatgc cgttaggcgc tttcagccag
     2701 atgtagggag cgttcacacc gccggatacc gtgaagcccg cttcctgcaa gcccttcagg
     2761 atatactgtg cgttgttcat gtaataagca acctgctcct tcagctgtgc cttgcctgct
     2821 gcagagtata ctgcctcacc ggcgcgctgt acgatatagg gtgctccatt gtatttggtg
     2881 ccgtgtcttc ttgcccacag tgcatggagg ctcacatccc cgctctttaa atccttgggg
     2941 atcacggtaa agcccagacg cactccggta aagccggcat tcttggagaa ggaacggaac
     3001 tcgatggcac aggttctggc tccttcacat tcatagatac tgtgaggcac atccggttcg
     3061 gaaatatatg cttcgtatgc cgcatcatag agaatgacag cgcctatctt attggcatag
     3121 tccacccact cctgcagctg cgccttggta atggtggcac cggtaggatt attgggaaag
     3181 cacagataga tcagatccgg ggtctccttc ggcagctccg gtgcaaaacc gttctctgcc
     3241 agacagggca tatagatcac atcactccag gtctcggact tcggatcgta agtgccggtt
     3301 ctgcccgcca taacattaga atctacatat acggggtaaa cggggtcaca tactgcaatc
     3361 ttgttgtcca gactgaagat ctcctgaata ttgccacagt cacattttgc tccgtccgat
     3421 acaaagatct cgtccggtgc aatgtcacag cctcttgcct tataatcatt ctctgcaatg
     3481 gcattgcgca ggaattcata accaaggtcg ggagcgtaac catgaaaagt ctccgctttt
     3541 cccatctcat ccactgcacc gtgcagcgca tcaatgatag cgggtgccaa aggctgtgtc
     3601 acgtcaccga tgcccagacg gatgatcttc ttgtccgggt gtgctgcgga atacgcactt
     3661 acctttttgc cgatggtgga aaataaatag cttccgggaa gttttaaata gttgtcgttt
     3721 actttaaaca tagtacgttc tcctctctat tatccctggt gcagtattgc tgtcaccttg
     3781 ctgtcgtaaa taagcaattt ttccgcacta cgtgctctca aaattgcaac gaacattcgt
     3841 aatccgggct gatcacccta cttacttatg ttcgtttact cctctcatag aaacatgtct
     3901 ttgtctgcaa gcagccacgc cgtgtttcta tggaggagtt tattctatca gatggagtac
     3961 gggtccactt caccttcaaa tacgatcttc gcaggaccgg tcatgtagac cagattctcc
     4021 ttgcggtccc atttgatgtg cagttcaccg cctaatagtt taacagttat ttcctcgtcc
     4081 gtcaaaccat ttagtacaca agccaccgcc gtggcgcaac atccggtacc acaggcaagg
     4141 gtctccccgg tgcctctctc ccagacgcgc ataaacacat ttttccgatc cagaatttct
     4201 acgaattccg tattggtccg gttggggaaa caggaatggt gctcgaattt cggaccaatc
     4261 ttctcaatgt caagatcctt caccccgttc ataaatacaa tggcatgggg attgcccatg
     4321 gatacacatg tcatgtggta ttccgtgtcg tctaccatga tgggagcatt cactaccgcc
     4381 ggtccgtctg ccgtctcctg tgtggcttcc actgttaccg gcacctctgc cggagcaagg
     4441 atgggatttc ccatgttcac ctgtaccagc gtcacctgtc ctctgtccgt ggcggtcttt
     4501 ccctccacgg tgagtttcag atattttatt tttccggcgc tgacgatggt aatgtccttc
     4561 ttgtccgtca gtcccttgtc gtagacatat tttgccacgc agcggatgcc gttgccgcac
     4621 atctccgaac ggctgccgtc cgcattgtac atttccatct caaaatccgc ttcttccgcc
     4681 ggattgataa agatcacacc gtcgccgccg attccgaaat gtctgtcact taagcttctt
     4741 acgatctccg gcttctcttc cggtgaaagc ttctccgtaa agccgttgac atatacataa
     4801 tcgttgccgc agccctgcat ttttgtaaat ttcatggatt gctgttcctc ctgttgttct
     4861 ctttcacata tcctgtctgt aacgcccgga tccctgtaat catttccggg ccgtcctgct
     4921 tactccgtca tcatggacaa taccatctgt gccgtctcca ccagattggt accactgtcc
     4981 atactggtct tctgaaggta gcggtgggct tcttcttccg tcatatgatt tcgtgccatc
     5041 agtaattcct tggcatcctt gattgctttt tcatccgctg cattccgcac tttcggctgc
     5101 atacggcgtt ttcttctgcg gcgttccaca ccttccacca tcaggttcac ggtattgatc
     5161 aggtcctgta cctttaacgg catggaaaga catacgatgc cattgccata gcattcactg
     5221 atcagattct gggatgccat cagcagcatc tcgaaacctt ccggcagatc ttcatgtagt
     5281 tctgtgtata tcatgtccgc cagcttgtag ctgcagatca caataccgtc gttcagaccg
     5341 tccgcctgac tgatagcctg agtgcctgtg gtacagatac ccgtgacctg aaagccactg
     5401 cgcaccagaa tattcttgac gcctttggct tcctccagct tcggcagtac gacaataatg
     5461 ttcgtcatcg gcccacctcc tttcttctgt gttttttata tcatcctaca aaagaaagga
     5521 ctcttttaat acttgtacag atactcctga atctcccact cggatacctg agaacggtac
     5581 tcatgccact cttttcgctt ggcatcgatg tattttccgg caatatgatc tccaagcact
     5641 ttctgaatga aagtatcctt ctccagttcc tccactgcct ccagaagtgt gccgggcagc
     5701 tgatcgatat gcaattcctc acgctctttt tctgacaggg caaaaatatt ggcattgatg
     5761 ctctgaggcg gctctatttt ctcccggatg ccctctaatc ctgcactcag gcagactgcc
     5821 agtgtcagat aagggttagc agcagaatcg ggactacgca gttcgatacg ggtgccctct
     5881 ccgtctgcag cagccggaat acggattaag gggctgcggt tcgtcgtgct ccatgcaatg
     5941 tagaccggtg cctcgaaacc gggtaccaga cgcttgtagg aattcaccag cggattcgta
     6001 atggctgcca ttcctttgat atgtctcatg ataccgccga tgaaatagta agcctcttcg
     6061 ctcagaccat tgggatcgga agcatcctgg aaaatgtttt ttccatcctt ctgtaaggac
     6121 atattgatat gcataccgga accattgaca ccgaacttgg gcttcggcat aaaagtagcg
     6181 tgcaatccat gacgtctcgc aatggtgcgt acggccatct tgaaggtcat gatgttgtct
     6241 gcggtatgta atgcctcatc gtatctgaaa tcaatctcat gctgggccgg agccatctcg
     6301 tgatgggaag cctcgatgac aaatcccata tcctccagat tcatgacaat gtcacgacgg
     6361 gcattttcgc cgaaatccag gggaccgatg tcgaaatacc ctgcctgctc atgggtgttg
     6421 gtggtaggca tggtattttc gtccgtatta aataagaaaa attcacactc ggggcctacc
     6481 tcaaaagtat atcccatgtc ctctgcctgc tgcaccgcac gttttaacac ataacgagga
     6541 tctccctcga aaggctgtcc gttgggacga tatacatcgc agatcatacg ggccactttg
     6601 ccctgctggg gtctccaggg gaaaatctcc agtgtcgaca gatccggata cagatacatg
     6661 tcggattctt caatccgtgc aaaaccgtcg atagccgaac cgtcaaacat acatttattg
     6721 ctcaatacgc ggtccagctg gctggctgtc accgcaatat ttttcagatt gccgaacaga
     6781 tccgtgaact gcagacggat aaattccaca tcctcttctt ccacaagacg gatgacgtct
     6841 tccttggtat aattattcat cgttaccctc gtttctgcgc cgcctgtctg ctcctcttcc
     6901 acggggcgga cgcatccgtg gaaaaactta ttattaacta tagcaatgcc gcttctgtct
     6961 tgtcaatgcg aagttctcca catttacagt actacggaat acggctgtcg catactattt
     7021 ataaagatca tcccatcatt ccaaggagac ttaagaatga gtgccaatac aaagattgtt
     7081 gtccttcgca gaaaagagct gctgtatacc ggaatcttcg ctgcgctggg agtgctgttc
     7141 gtcatattac tgttaatgtt attgctgccc ggaaaagctg cggatacctc cggggcaccg
     7201 gattcccctg acagtactgc cgagaccgtt atgccggaca atgtggcgga tctcggacgt
     7261 atcccgcaat ccgcacagac ggaggatgtc tccaccgggg tcgttgccga taccgatgct
     7321 gttctggata atgcttccgg gcctgtcggt actgataata catatatccc ggggatctac
     7381 accacggagc tgatcctcgg cagcgagacc gtgaatgtag aggtcatcgt aaacgaccac
     7441 gccatcacat ctttatgcct ggcagatccc gatgagaccc tgaccaccat gtatccactg
     7501 ctggagccca ccatggattc cctgagtgag cagatctgcg agacgcagga tctgagtcag
     7561 gtgacgtatt ctgcggagac cagatatact tccctggtgc tgctggaggc tgttaaggct
     7621 tcactggaaa aagcaaagcc cagtgccaca ccggaagcga cggatgcgcc ggatgcaacg
     7681 aatgcaccgg cagcctctcc gaatgtaccg aaagcggata tcaatcctgc ggcataaggg
     7741 actcatgtgc cgt
//
LOCUS       DAWDHD010000115         7664 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_561_length_7664_cov_8.976869, whole genome shotgun sequence.
ACCESSION   DAWDHD010000115 DAWDHD010000000
VERSION     DAWDHD010000115.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 7664)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7664)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7664
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_561_length_7664_cov_8.976869"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1738 a         2172 c         1580 g         2174 t
ORIGIN      
        1 gcatgctccg ggagttcgtg gattttgcca atgtatttca ctaactctca atttattgaa
       61 tcaaatcata gcacataatc ttctgctgta tctgcgcata agcctccggc tccagatcat
      121 atcccagata agcaattccc tgatacactg tttccggaat gatctccgat gaactcattt
      181 ttttacaaat agcctcaatc agatcccgct ttgccgcgat gatcttcgca tctgctgcgt
      241 aaaattgcgc catatcgtaa tctgaagtat tgccacaggg gttgagacag ttagcgcagt
      301 ccggtgcaat ggcgaatttc tccgcatgaa tcttctggac tgtctcttct tcgctatcgc
      361 catctgtcaa ccgcagaaat gcttcccgga ccacactgtc cgtccgctct gttttgccat
      421 tattagacat ggctcccacc agcccgatca gggcgctgat gattctgtct ccctgcattt
      481 tcctacaaac ctccattttt cagatgcctg ctttccgtac agacaatcct gtttattcca
      541 aaatctctcc gtcaatggag atggtcacta cctgccaggg caggaacttt ccgctgtcct
      601 gtatagcctt ctttgccgcc atttccagtc ctccgcagca gggcacttcc atgcgcacga
      661 tggtcacgga ctgaatgtcg ttgttccgca gaatctccgt cagcttctcg ctgtaattca
      721 ctgcatccag cttcggacag ccgatcagcg tcactttgcc gcgaatgaac tccttatgaa
      781 aatttgcata agcatatgcg gtacagtctg ccgcgatgag cagttttgcc ccgtcaaaat
      841 acggtgcctg caaaggtgcc agtttgatct ggaccggcca gttacacaac tgggatttca
      901 ttgtcgcact actctctgcc acaatttctg cgttctccgt ccggttcatc tgacgcattc
      961 tgcttcctgg gcatcctcca tggggaatct gcataccttc ccgttgcatc ttttcctgtt
     1021 gtttcagctg ctgtgcctgt tttactgccg cctcatcata ggccggcgcc tcccgctctt
     1081 caaaagtgat ggctcccgta ggacatccag gcaggcagtc accgaagcca tcgcagaaat
     1141 gctctctcac cagctttgcc ttgccgtcaa taatatctat cgcaccctcg tgacatgcgg
     1201 gcgcacaaat cccacaacca ttgcattttt cttcatcaat atgaatgatt tttcgaatca
     1261 tgttgcctcc ttaattttgc tgttctcttt gtctcttcga ataccataac agacttaagc
     1321 cggatgttat gttgtttttc caacaaaatc attattttct tcgcatacaa aatcatcttc
     1381 tccagcgtcc tgcgattaat cgtaagcagc gcttttcctt ccagcttatg accaatggcc
     1441 tcataaaaag aaccgatagt gaaccagtct aacctatggg caccatcatg ttctcgccca
     1501 gctccagcgg taaaattctg aacacaaagg gcaaaaaaat accgcctgca tgaatattag
     1561 ttccacacaa gcggtatata cagcacgact tataatttag aaagacacgg tcagctgcat
     1621 cttgaatctc tcttcaccgt atactgcgtg aggaatatcc ttgggcatga tcagtgtctc
     1681 gccctcttcc acatagaata cctcgtcgcc tacggtaaat ctgcccttgc cttccagaac
     1741 ggtcaccatg gcatctccgc ctgctgcatg ggtagatatc tcctcgccct tgtcaaagga
     1801 aaagattgtc atgctcacat aatcgttctg taccagagtc ttgctgacta cctggccctc
     1861 ctggtattcc accagctcct tcagagttaa tttggtctgc ttgtcgatat ttttatacat
     1921 cgttgtccat cctttcttaa acacatcgtt attgtgcctg cccggcatgc tcccaccaga
     1981 gctctccttg actttctgag gtgattatac tagaatcatc ccatcaagta tgttgatttt
     2041 ccaacaaaag gagaattcat tatgactagc actctttttc aaaatatttt acccgaagat
     2101 atccctgtta tgctgaagcg tctgaatgca tatacgaaat cctatcaaaa ggaagaatac
     2161 atcaagcatg ccggtgatcc tgcggatttc atcggcatta tcgaatccgg ctccgttcac
     2221 atcctacagg acgattatta cggcaaccgc aatatcaccg cttctatccc cccggattcc
     2281 ctgtttgggg aggcttttgc ctgtgcagga attccatatc ttcccgtgga tattgtggca
     2341 gcagaggact gtacagtgat gttcctaaat gggaaaacac tgttaaacac ctgcgacaac
     2401 agctgcactt tccatcatac cctgatacgc aatctcctag gaatcgtagc gcagaaaaat
     2461 atgtatctga atcagaagat caaatatacc tcccgtaaga ccacccggga aaaactgatg
     2521 gcctatctta ccgatcaggc aaaattgaaa aggagcaacg attttaccat ccctttcaac
     2581 cggcaggagc tggcggatta tctcggggta gaaaggagcg ccatgtccgc agagctgggc
     2641 aagctcacaa agcttggcat cctccggaca cagcgcagtc atttcacctt attaaaacca
     2701 ctggattaat ttttgatcac cttttcaaaa tcggaagctg ggtgtttgca cagcggacag
     2761 ataaaatctt ccggcagttc ctcgcccacg tattcgtaac cgcagatggt gcagcgccat
     2821 accgtctggc cgtctgcggc ttttcccact tcctccggct ttggctttac ctcttcttga
     2881 tagtactcat agtttgccga tggctcctcg cttaacactt ccatctctgt cacttcacca
     2941 atgaacatgg tgtgggatcc cagatccacg gtctgactga cctttaccgc cagataagca
     3001 tttgtgcctt ctatgatgta gggcacgcca ttctccgcat ttttatacgc tgtgtagtct
     3061 gcgaatttgt ccacttcccg gccggattgg aaaccaaagc gctgatatag agggaatagt
     3121 gctgtcttac taagcaccga tacgttgaac acgccggtct ttaacaccat atcatgggta
     3181 taatttgcct tattgatgca tatgcagagc tgattcggag tggatgccgc ctgaatcgca
     3241 gtattgacga tacaaccgtt tgctttctct ccatctttcg tcgttacgat atataatcca
     3301 taacttaact tgtgcattgc cttgttattc attatattct ctccttttct ccttcagcct
     3361 gtttcgcttc ctttttagta tctcttatct accatatttt atttgcttaa catttcttat
     3421 tttctttatc ctgataataa cacagttgga attatctgtc aataatatta gtaatgattc
     3481 ttatttttat ttctatgatt taaaaacacc gatacctttc gataccagtg tttcttatct
     3541 gtttcctaat aggtcacaac gttatgatac actctgtaat atccgtctcc gatatccacc
     3601 agctgcagct gtattgcaaa atactccata ccgagatttt tcaacacatc caccacatat
     3661 ccctgctcgt aggctccggt atgataggat tgctcaatgg tactccacag gctcttaggc
     3721 acaacattgt caaaatgctg gtcaccactt cccaggccgg tcagctgctt cctgcagtct
     3781 tcataatagg aacttaaatc cgtcagcgca tcggctctgg gctccggcgt agcggcagtt
     3841 gtggtattgc ctgcagagcc tgcggtattg ccggagggat aacgcaacgg ctcctgagga
     3901 tccggattga tataatcact gagagagctg ccaggctgct ccggttcctg cgtaccgtct
     3961 ccctgctgtc cgggatctgt aacgctcccg tctgtctgac cgccggaggg cgtggtgcct
     4021 gcgtccggat ccggtgtggc tccgcctgct tcagacggct gaccggtacc cgctgcccct
     4081 gtggtatttt cctgtctgta agcggtaccg ttgcaggccg gcagatacac cgacagattc
     4141 tgtcttacat gggtggaggc aaaatctgca tccgtcttgt taaaatagtc atatcggatg
     4201 gccgctgcgt catcccaggt cacatccaca ttgtaataac catcctccag ctttacgata
     4261 ttccaggcat gatccccacc ggaataaccg gtgcagtaat aacagggaat tcccagctgc
     4321 tgtaacagat actgataagc tctggcataa ccggcacaca cgctcttccc attcaccagt
     4381 gcactgtaag cactctggtt catatccgct gtcagatcat aggttactgc cgatgccagt
     4441 gcatcatgca catatttctc tttttcataa ttgctgtcaa gggacgctgc cccggtaatc
     4501 agattctgtg ccgccgcatc aaatctttgc tttgcgctct ccagatcatc tgccgtaccg
     4561 ttatatttta aatcgatctc cacgcattgc ccgttggcca gatatttgct ggaataaccc
     4621 gtctccagcc agaacatttc cggatgatca ccgatcaccg cctcaaacgc tgtctttaca
     4681 tcactggcag acacggtctt ctccggtgca aacttttccg tcaggttctg tgcgttggca
     4741 taaatctgtc ggtatacgga ctgctgattc tccgacaaca tctgataata cggatactcc
     4801 tctgcggaaa atgacagccc ctctcccgtt tctccggaac ctaatgtttc cttcagattc
     4861 tgtgcttcct gatccggaat ctgctgttct tccggctgca cgggagtaaa tcctgtcctg
     4921 ccgctgacat cctccggaat ctgcatctga tccatggacg gcgccacgta gccgttcatt
     4981 tcccccggca taccattagg aagcgtcaca ctgatatttc cattcccgga agtctgtcct
     5041 tccccggtct cttcggtccc agggggcata tccactgtat tgccatacag tttctgcgcc
     5101 agagaactgg tcatatcggg gttcaatgcg aataccagga ttccgccaca caacagcaga
     5161 cacaacactg caattcctat catgatctta ttgactactt tcatcttatg ctcctatcag
     5221 accgggctcc tggcctgtgc tcctgcccgg atttgttata aagccagctt ctgccgctat
     5281 tgcttctctt ctcctgcggc aagtaatcca tccggagaat agcggttcgt tgcattccac
     5341 aggatccatt cttcatatcc cgcatcgtag actgcctgga tctgttctct gatctgtgtt
     5401 ccgttgtagg aaatatgtcc cggaacccag gtggccgtaa atgcctgcag ccatggccgt
     5461 actacagccc tctccgcttc cggaatctcc tgcaattctt ctgcagaccc ctgcatggcg
     5521 gtcgataccg tttcgtaagg atgggcatcc ggcaccttca gaccgaacac accgttagca
     5581 taatgagagg gatataccat agggctgatg gcatccactg tctgtcccag tgccgtgtaa
     5641 tcctgcccta cggtctgcac atccgtctcg ctgccgataa tagtgccgaa gacatctgcc
     5701 gtgaccacac agcccttttc atgaagtctg tctcccgcat aagaaaggaa atcctgtatg
     5761 gcctgccgct tcggataagc ttccatatct acaccgtaat ccgccgcatc ggcatccgaa
     5821 cctaccggga atcgcacata atcatactgg atctcatcaa aacccagatc tgccgccatc
     5881 tccgcaagct ctgtcagata ttcccagacc tcctgcctgt agggatttac ccaggccagt
     5941 ccattggcat ctgtcaccgg cttaccgtcc ggcttcgtca gtgccaattc cggctgtgcc
     6001 gccgccagaa tgggatcctt aaagcatacg atccgcgcaa tggtatatat attatgatct
     6061 ttcaattcct gcatcattgt ctgaatgtcc cggatgtaac gtattccggc ctgcatttcg
     6121 gatatccggt ccagcacggg aatgtcctgt ggaatctcct cattagtcag ccggaacgtc
     6181 aaatttccct cgtcgttttt cacgtcgatg accatggcat tcagttccgt ctcatccacc
     6241 aggcggatca gatcttccat tccggcactg ccagccttcg ggccggtgac gtagattccc
     6301 tttaccttcg tccgtcccgc catgcggtcc gccgtagtct gctctgcatc cccggtggcg
     6361 gcatccccat ccgtgacatc cggtgcattc tccccggtgg cagcctcttc tgtgaaatcc
     6421 accgtggcca ttacttctat tcctgtctct tccgtccctg tcagagataa ttcattgtct
     6481 gttccacagc cggtcatgcc tagtataagt acaatccctg ctgccagaag tctcttcccc
     6541 ggaccagtat ttcgtatcat cggtactctc cctgcatacc gccggtcgct cctgttttct
     6601 ttatccagcg gtacaaaagc ttcataattt catggttctg aatagttccg tcggagtcaa
     6661 aaggtctttc acccacttat gagcaagctc gtgtgggctt agaccttttg cccctggtat
     6721 cattatattt ttaattgttg aaaaatgcaa gaaatgctat actatatcta tcggctctgc
     6781 ctccgcttga gaaaagagcc gtcctgattt tccctggaat aaggtttttc accacaagaa
     6841 attaggtctt ccatgtatca taattttatc tttgatctct acggcacctt agtagacatc
     6901 cacactaatg aaaataaaac ttccctctgg aaaaggcttg cgctgttcta ttccttccag
     6961 ggtgcttcct atacggggag ggaactgagg aacagatatc gccagctgga aacggctgcc
     7021 agcaaggttc cctcatctta cacctgtccg gagatctgcg ccgcagatat ttttcgaact
     7081 ctttatgtcg aaaaagggat ccaacccaca gaggctcttc tgaccgctac cggtcagttc
     7141 ttccggatcg aatccctcaa tattttacaa tgctatcccg ccacgatcgg cacactgcag
     7201 cagctccatg caaaaggctg tgggatctac ctcctgtcca atgcccagcg gatcttcacc
     7261 gaatacgagc tgcgctcgct gggactcttg gatctgttcg acggtatcct tatctcctct
     7321 gacgaggggt gctgcaagcc ggatcccgct ttttttcaga tcctgtttga ccgctacgga
     7381 ttagatttca ccacctgtct gatgaccggc aacgaacgca ccagtgatct ggcaggtgct
     7441 atagctgcgg ggatcgactg ctgttacctg cacaccgcca cctcttccgg ttccgcagat
     7501 gagccaccaa aaaaagccct ctttacagtt atggatggtg actcttatgc gctgcaaaaa
     7561 cttcttctaa gtttagcgag ataagataaa gcacccggaa gtcagctttt gtaccgaccc
     7621 ctaattgtta gaccaaaaat ctaacgattg gaggtcggta tttt
//
LOCUS       DAWDHD010000116         7561 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_570_length_7561_cov_10.700773, whole genome shotgun sequence.
ACCESSION   DAWDHD010000116 DAWDHD010000000
VERSION     DAWDHD010000116.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 7561)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7561)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7561
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_570_length_7561_cov_10.700773"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2334 a         1464 c         2155 g         1608 t
ORIGIN      
        1 aagtccaaga tcgaatcctt aagcggtgaa gtggaagtga aatccaagtg gggagaggga
       61 agtacctgga ccattcgact gcctctgacc cttgcaatta tccaggcact gatggtagtc
      121 gtaggaggag aaaaatatgc gatctctctg ggatccatcc agacgatcga agatatctct
      181 cccaacgata tcaaactggt agagaacaaa gaagtgatca acttgagagg aaccgtgatt
      241 cctctgatcc gtctgactga ggtactggat gtagagagta ccagatcccc tgaggacaac
      301 ctgatcgttg tcattgtcaa gaagggcgat aagatggcag gtctggttat cgacgaactg
      361 atcggacagc aggagatcgt tataaagtct cttggcaaat acatcaagca gtgcaaattc
      421 atcagtggtg caacgatcct tggtgacggc gaagtagcac tgatcctgga tgccaacgca
      481 ttactgtaag gagggagatg acagttatgg aagaacttag catgaaagaa actttaaagg
      541 ccggagacga gcgtaaagtt gaggaggatt ttttccagta tattgtgatc cgcctcggag
      601 atgagcagta cggtatcgat atccgataca ttgacaatat tgtaagaatg cagcatatca
      661 ccagagtgcc caaggtaccc gcatatttaa aaggtgtcat caatctgcgt ggtgaggttc
      721 tgccggtcat gagtcttcgc gtgaagatgg gactggaggc agacgaactg acccgtgcta
      781 ccagaatcat cattctgaag caggaacagc agggcagtgt aggaatcatt gtggatgaag
      841 taagagaagt agtgactctg ggaagcagtg agattgagaa attatcacag aataccgctg
      901 agagcaaaaa gagctttatt acaggtgtag gcaaacacaa tggggaactg atctctctgt
      961 tggatctgaa ttccatcaca ttggaggaaa atgcataaag gagactgatc catgggagat
     1021 ctgagtttag agaaagtttc ggaaacatat ctggacgttt taaaagaaat tggcaacatt
     1081 ggagcgggca acgctatgac agcactctcc cagatgctga actgtaaggt agatatgaag
     1141 gtaccgcagg taaagctttt ggatttcaac gaagtaggag cattgatggg aggagaagag
     1201 caggtaatgg tcggtgtctt cctgggagtg gaaggagata tcaccggcag catgatgttt
     1261 ctggtagaac agggcagcgc caagcatctt ctccataaga tcatgggaga catggtacag
     1321 caggaagggt tctccgagat cgaattttct gccatgcagg agatcggcaa cattattacg
     1381 ggagcttacc tgaattccct gtccatgatg acgaatctga ccattattcc taccccaccg
     1441 tctctgacac tggatatggc aggagctatt ctcagtgttc cagccattga attcggaaca
     1501 ttgggagaca agattctgct gatacaatct cagttttatg acgaagttga gatcgacgga
     1561 tattttattc tggttcccga tattgaatcc tacccgaaga ttctgacatc tttgggaata
     1621 cctatagcat agattcagaa gcgcagaaga aggggagcag aatgagcgag attattaaag
     1681 tagggatggc ggacctgaag acctgtgtaa gcccagatgg agtgacaact ctgggactgg
     1741 gctcctgcgt gggaatcgca atccgcgacc cggtgacaaa aataggcggt ctggcacata
     1801 ttatgctgcc ggacagcact acgatccgta acagcagtca gaacattgca aaatttgcag
     1861 ataccggaat cgatgaactg gtacgacaga tggagaagct gggagccaaa aaagcgagaa
     1921 tggttgccaa gattgctggc ggagctacta tgttcacgtt tcaggggaaa aacgacatga
     1981 tgcaggtggg ggaccgtaac gtggaggctg tgaagaagaa actgaaagag atcagtattc
     2041 ctattttggc acaggataca ggtaagaatt acggaagaac cgtaactttt tatccggaga
     2101 ccggagagtt ccatattcgt gcggtaggaa agagtgagtc catcatttaa ggatggatga
     2161 ccggatgggt aaattatgaa cagaaagaat atgccattgc ttttgatgct gatcgcaggt
     2221 gcgtgtacct ggatcgtaac gctggtgaga agttattcgt tactggcaag tcttgtggca
     2281 ctttttactg ttatgctgct gttttattgt ctgggaagtg tgatccggct gttgctggac
     2341 cggttcgaca gacagaatga agagaaggca aaacaggagc aggaagaaaa agagaaggct
     2401 ttacaagagg aagaagctgc tgggtcacag gaggacacag cgcagaaggg tgtaagcgat
     2461 tcgaatggat gaagccggta agaaaaggtt attagaagaa tatgctgcta ataaacgacc
     2521 ggagaccaga gaacagttga tactggagta tgctccgcta gtcaaggcag tagcgggacg
     2581 gctgagtatg tatctgggct ataatgtgga ttacgaagac ctggtcagtt acgggatttt
     2641 tggactgatt gatgccattg acaagttcga ctgcctcaaa gaggtaaagt tcgagactta
     2701 tgccagtctg aggatccggg gagccattct ggaccagatc cgtaaaatgg actggattcc
     2761 ccgaacgata agacaacggc agaagaagat cgacgcggtc atcaaggaag tggaacagac
     2821 cacgggacgc agtgcaacgg atgaagacat tgcagcggga ctgggaattt cttcagagga
     2881 atatctggac tggcagtccc agatgaagat caccggcctg atatccctga acgagtatat
     2941 ggatcaggga agtgatgtct cacaggatta tagcagacat atgacagcac gttttgatgg
     3001 tccggaagaa agtgtggaaa aagaagaact gaccaaggtt ctgggagaag cactggaact
     3061 tctgacggaa aaggaaaaga aagtagttac gctatattat tatgaggatc tgacattgaa
     3121 ggaaatcagt aacattctgg aggtgtcgga gtccagggta tcccagctac atacccgtgc
     3181 tttacagaaa atgaagacaa aaatgggaga ctacatggga cttttgtaga acctcgttga
     3241 cccgtaatgg aatgcgggat gggaaggcag aggaaaaggc gtatgcagaa tggctatttt
     3301 caagtggtga gaacaccggg aggttatgga ttacagatct tccaacccaa ggatggagga
     3361 gaggacgtaa gactgcagga ggtaatggat tatctggaca gacaatccat accttatgat
     3421 tccatgtcca ttaaaaaggc gatactcgca gaagaagacg cggtctgctt tctggaacga
     3481 aaggattgtc cggaaatcga ggaatcctgt cgtatagaag tcagtgagga caatttgcag
     3541 gcaatcgtac ggttctatcc ggcttcacag accgggaaac gggcaacaat ggacagtgtg
     3601 atcaaagatc tccggttccg gaatattgtc tatggaatcc agatgagtgt tctgcaggat
     3661 cattttatgt ctgacggaat ttattgcacc gatctggtag tggcccgggg aaaagaaccc
     3721 agacatggca cagacgccta tatcaaatat tattttaata cggatgtcca tgcacagccg
     3781 acactgaaag atgatgggac ggtggattat ttccatctga acatgatcaa cccctgtaaa
     3841 aagggacagc tgctggcaga ggtcattccg gaggacgagg gagagtacgg aactaccgta
     3901 cagggtgcca agatccggcc cagacaggtg aaaaaagccc atctggagtt cggtcataat
     3961 atagagattt ccgaagaccg gagaagactc acttccctgg tagacggaca tgtttccctg
     4021 gtagagggaa aggtgtttgt atcggatgtt tacgaagtgg aaaacgtgga tttatctacc
     4081 ggaaatattg attttgaggg aagcgtacag gtaaatggca atgtttcctc caatttcgtc
     4141 atccgggcag gcggcaatgt gatcataagc ggtgtcgtgg aaggtgccta cattgaggct
     4201 gggggcaata tcatcattgc ccgcggaatg aatggtatgg caaagggtac gctgaaagct
     4261 gggggcaata ttgtggcaaa attcctggag aatgctacag ccagtgccgg aggctatgtc
     4321 agcacggagt cgatcctgca cagcaatgtg atagcggcaa cagagatcca ggtaaccggc
     4381 aagcgaggat ttatcaccgg cggtcatgta cgtgccggac agaagataga ggttaagacg
     4441 ctgggggcaa tgctgggagc gccgacggta gtggaagttg gtgtggatcc ggagaaaaag
     4501 gcagaatata tgaagatgca gaaggaagtc tccgagattg tgaagaatat ccgcagtctg
     4561 cagccgatcc ttgcgaattt tgcagaaaaa aagagcaagg gagtccgttt tacaccggat
     4621 cagatcaact atctcagaaa cactgcagct tccatggaga cactcaataa gagtctggag
     4681 gaaaagaacc ggcgtatgca ggaactgcag ctggagttca atccgcagga gcgggccatg
     4741 gtgcaggtac ggggagaagt gtacccgggt actacgatca tcatcggaga ttcttccatg
     4801 caggtgaaga gtacctatca ttattgccgg ttcgagcgca ttgacggaga tgtgaagtcc
     4861 acaccactgt aaccggcagc agattgttgc aggaacagaa aatggcagaa aagaggaatg
     4921 tcttatgtcg tttggatctt tggaggttgc gtcgatcccg cgaacgcagg attacacgac
     4981 gatgaagcag aatgaagaca ataagggtgc atttcaacag atgacgctgg gagagcatgc
     5041 acagaaacag agtgaacagc ggaccagaga agtgcacagc agtgataatg cagactggta
     5101 tacggggcag tatgatgcca aggaaaaagg aaagaatgag tatgccggta acggtggtaa
     5161 acaccggaaa aaagaaaatt ccgaaaaaga tggtgtggtg gtgacgaaac agcatcaggg
     5221 atttgacatt aagatctgag aatacaaagg aaaagcagta tgggtatatt agaagtggtt
     5281 ctgctgattg caggaatcgt aatatttata ggaagttttt tattgccttc cggaaaagaa
     5341 agcggaatca ataaagaagc agcaaaagaa gagatcaaag gtcttatgga agaagaaatg
     5401 cagacagttc gcagtcagat gcaggataaa atggacgaga cctcggaaga tgcgctggag
     5461 aaggcggaac gttccctgga acgtctgacc aatgaaaaga tcatggcagt gagtgaatat
     5521 tccgatacgg tattgcagga gattcacaag aaccatgagg aagccatgtt tttatacgat
     5581 atgctgaaca ataagcatgc caatatcaaa gataccgtat ccaaaatgga taaggcagtg
     5641 aaggcggcag aagacaaggc taaggtcaaa gcggaagaga acgtgaagac acagaaggag
     5701 aatgtgacgg aagaacagct gatagaagta caggaaccgg cagagacgat agagccgtcc
     5761 ggctccccgg agatcggatt catgggggag actgcagaag aaggtcagaa caataacgag
     5821 aagattctgg aaatgcacag acagggcaaa tccacagtgg cgattgccaa ggaactgggg
     5881 ctgggtgtcg gtgaagtaaa gcttgtgatc gacctgtata aggcctgaca gtaatcgtgt
     5941 gaattaagga cggagatatc gatgaaatta aaatataatc tgcgaggtct cggaatcggt
     6001 atgatcgtga cggcattgat cctgggaatc agtttttcca accggcagga tcagacatcc
     6061 cagatcatga cggatgacca gatcagagag cgtgcggcag aacttggaat ggtggacagc
     6121 agtgaactga cgctggcggc actgcaaaac agtgcaaaac agccgacgga aggcacaccg
     6181 gaggaaacca cgcagacaca ggaacagaac aatatagaag cggaaccgga gaccacggtt
     6241 cccgcagaga cacaggctac ggtggaaccg gagacaactc aggaaccgga agctacagca
     6301 gagccagagc ctgagaagac ggcggaacct gaaaagacgg cggcaccgga gacaaccgca
     6361 gagccggaag taaccgaagc accacagcgg acacagaccg ccagcataac aatccagaga
     6421 ggcgatgatt ccggcagtgc cagcagacgg ctctatgagg ccggactggt ggagaatgca
     6481 aaggcatttg acaattattt atgcaacaac ggctacagcc gcagtatcaa tcccggcaca
     6541 tatgagatcg ctcccgggac ttccgaagag gaaattgcta aaataatcac aggaaaacat
     6601 taaaaacccc ttgtcatagg ggttttccta tgttataatc gcactgttgt gacaaaaaac
     6661 acgcctttcg atttgacgat ggtgctcctg ccaaaatgca gaggtcttcg ccagagctaa
     6721 ccgggttctt aacgcgtccc ggcagaaaag aaatgcggaa gattttaacc aaatggaggt
     6781 aaagacatga gcgttatttc tatgaagcag ttacttgaag caggtgttca ttttggacat
     6841 cagaccagaa gatggaaccc taagatggct ccttacatct tcaccgagag aaacggtatc
     6901 cacatcatcg acctgcagaa gtccgttgta aaggttgacg aggcttacaa ggcagtatcc
     6961 gagatcgccg cacagggtgg cactattctg ttcgttggta ccaagaagca ggcacaggac
     7021 gctgtaaagg ttgaggctga gcgttgtggt atgtactatg taaacgagag atggttaggt
     7081 ggtatgctga ccaacttcag aaccatccag tcccgtatcg caagactgca tgcaatcgag
     7141 accatgtctc aggacggtac tttcgatgta ctgcctaaga aggaagttat tcagttaaag
     7201 aaagaatggg agaagctgga gaagaatctg ggcggtatca agaacatgac cagagttccc
     7261 gacgctatct tcgttgtaga tcccaagaag gagagaatct gcgtacagga agctcatgca
     7321 ctgggcatca ctctgattgg tatcggtgat accaactgtg atcctgaaga gctggattac
     7381 atcatccccg gtaatgatga cgctatcaga gccgttaagc tgatcgtttc caagatggct
     7441 gatgctgtga tcgaagcaaa gcagggcgaa gctgtataca gtgacgctga cgttgctacc
     7501 gaggctgagg atatcgaaga ggttgcagct gacgctgaat aatccgattc cataagaaaa
     7561 g
//
LOCUS       DAWDHD010000117         7545 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_571_length_7545_cov_12.986382, whole genome shotgun sequence.
ACCESSION   DAWDHD010000117 DAWDHD010000000
VERSION     DAWDHD010000117.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 7545)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7545)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7545
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_571_length_7545_cov_12.986382"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2159 a         1530 c         1906 g         1950 t
ORIGIN      
        1 gaactattta tcgcattagt gagacgcagc aaagagaaga aaagcaagta aattccagtt
       61 tatagggatg atacaacaat tatttttgca tggaaaaaag aattcgacga gaaagaaaaa
      121 aatacctttg gaatggtaaa ttgaataaaa cggggcaaaa tattacaaat catattgaaa
      181 aaacggggca aaatagatac tgttaaaaag ggaggaggga atttatgtat agaaaagact
      241 ctattatcat agaagaatgg ttgaaaagat cggataaagc attattggta acaggcgcca
      301 gacagattgg aaagacctgg ctgataagag aggaaattgc caaaagcgga tatcgtaagt
      361 ttgaagttaa ttttatagat cagccggatt tggtggatta tctcaatgta aaaatgagtg
      421 cgaatgagtt cctggtgaaa ttaaaaatga ttatgccgga ggattgtaag ccgcaggaaa
      481 cggtcgtctt ttttgatgag atacagaaat gtccggaaat agtgacaaaa ataaaattcc
      541 ttgtagaaga aggcagcttc aaatatgtaa tgagcggatc cttattagga gtagagctta
      601 agggaataac gtcggttccg gttggatatt taactgtttt gagaatgtat ccgatggatt
      661 ttgaagaatt catgatagcg aatagtgtgt caaaaacaac tttggaaatg ttgaaagcga
      721 aatttgaaac atgtcagccc gtggatgaat tcatacatca gaagctattg tcgctgtttt
      781 ttatctatct aattgtcgga ggcatgccgg atgcggtaaa aatctatatt gctacaaaag
      841 atatccggga agtggataag gtacaacggg atatcgttgc gctatataaa gaggatttct
      901 ctcaacatga gagtgaagat aaaaaactga aattgatatc tatttatgat atcattccgg
      961 cagaattgaa caaacagaat aaaaaatttg tatttacaat gctgaataaa gagttaaagt
     1021 ttgacagata tgaaaacagc tttttatggc tgaaagatgc cggagttgcg ttaccggtgt
     1081 ataacgtgga agctcctgtt attcctctca aagcgagcaa gagcagtaat gtgttccgac
     1141 ttttttccaa tgacaccggt ttgcttacca gtgcatatcc ggcggaaacc aaactggaac
     1201 tgatcaataa aaacagcgaa gtaaataacg gtgctcactt tgaaaatgct gtggcacagc
     1261 aattaatggc caatggtttg gaaccttatt tctgtaaaaa gaaaaatata ggggaactgg
     1321 atgttttggt cgaaatggat gggaaagtgg ttcccattga ggtgaaatcc ggaaaagctt
     1381 acaaggcaca caaatccttg gataacttta tgaaaatttc tgattatcat attgagaaag
     1441 catatgtttt atcggtagca aatatggaac aggagggatc cgtggtatat cttcctatct
     1501 atatgtgcta tcttttaaaa gaacggaaaa ttggaaagtt aatcgtggat ctagacatgg
     1561 aagggttata acaccgggag aagtaataaa acgggatttg tcaaataaag gagcgaaata
     1621 gaaatgaaga ttcgcaaggc agaaaaaaag gacattccaa gaattcttgc actgttgggg
     1681 caggtattac agatacatgc agagatccgt ccggatattt ttataccggg tactacgaaa
     1741 tacaccccct acgagctggc agagctattg gaacaggagg atcaccctat ctatgtagcc
     1801 gtagacgagg acgatctgtg catgggctat gcattctgcc aactcaggga gcaacccttt
     1861 tccactaata tggtcccctt caaatcattg tttatcgatg acctttgcgt tgacaagcag
     1921 acacgggggc agcatatcgg ggaaagcctg tttgaatatg tgaaacagca ggcaaaagaa
     1981 ctgggctgct atgaagtaac cctgaacgtc tgggcaggca acacttcggc agagcacttt
     2041 tatgaaaaga tgggaatgaa gacaaaagaa cggcagatgg aatatatcct gtgagccgga
     2101 cggaggtggc aagcatgatt aaaattgcat tttttgatgt cgatggaaca cttctgaaga
     2161 tgggccataa agaattaagt gagcggacgc tgcatgcatt aatttctctt cgtcggaatg
     2221 gggtgaagct gtgtatggcg accggaagag gatatctgtc ggcgccgcgt tttccgggaa
     2281 ttgatttcga tgctcttctg acctttaacg gttcttatgt aacagtagga gacgagatta
     2341 tttttaagtg cccgcttgat cggatggaca agcttcagat ccttcagaat ctgaaaggca
     2401 tgcatagggc ggtagcgata agtaacgaac attttatcat caccaacggt acagatccgg
     2461 atctggagca gtatttcaga ttcggtaatg agatattgaa aatcgataaa caattcgatg
     2521 aacgggttcg tgaggatatc tatcagatga tgtgtgcctg ccgggaagaa gagtatgaga
     2581 ggattctcta tggtacgcac catacacaga ttacagcgtg gtgggataga gcggctgata
     2641 tcattcctct ggaatgtggg aagggaaacg ctgtgcgcgc ggtacttcag cattttggcg
     2701 ttacaaagga ggaagccatc gcttttggag atggcttcaa tgatatcgag atgctggaag
     2761 cagtcggcac cggtgtggcc atggggaatg cgaaggatga gatcaaagcc cacgccacct
     2821 gcacatgccg atcggtggaa gaagacggag tctacgattt ttgcttagaa catgggttga
     2881 ttacaatata ggcattgctg cccgcatttc cttcactttt ccggcatcca ctgcttcata
     2941 tgcattcttt cggtttctat cataagagaa gtccacgtcc tgcagggagg cggtcgcatc
     3001 cggctgccag cttctgcagg atgtatgaac ctgctctatg ctcgtatatt ccatcagagc
     3061 ttttacgttt tgtgcattca caccgcttcc ggctaaaaat gtgatatcct taccatactg
     3121 gctttgcagc ttttgcaatt gcgttctgcc cgaccataca tcgggtgcac caccactggt
     3181 gaggatccgg tccgcaccaa gtaagatcaa tgcttccacc gcacgttcct gctgcgcaac
     3241 acaatcaaac gcccggtgaa atacagcttc ttttccggcg ctatggatga ggtcgatcat
     3301 ttcctgtgtc cggttcctat ggatggcatg gtcctctgtc aggaagccga aagcaatacc
     3361 gtcagctccg tgctctaaaa gtatgcgggc atctaagagc atggtctcgt attcatcctg
     3421 ggaatagcag aagccgcctc ctcttgggcg gaccattgtt acaataggga tgctgcactg
     3481 ctcttttgca cagatcagat tggcggtaga aggtgtgaga ccacccagat acagtgcact
     3541 gttaagctcc accctgtcgg caccattttg ccaggcggtt ttcacatctt cgaagctgcc
     3601 gcagcagatt tccaataatc ctttcaaaat aatcccctca atcaatacat gacattattg
     3661 cgtatgactc ttgtttgcgg cttcattgat cagcaacatg attgtgttga tcgcatccat
     3721 ctggtggctg cggctcatgg cttctacata attctggcgg ttgaagtaca gctcatgcac
     3781 cttatccacc agcagattcg tcgtaatatc atcctcattc agcacaatgg aataaccctg
     3841 cgcttcgaag gaagcggcat tcaacagctg gtctccgcgg ctgctggcgg caggcagggg
     3901 gatcagaatg ttgggctttt tcagcgccag aagttcacag atggcattgg ctccggcacg
     3961 ggaaatcacc acgtctgcca tggcgaaaat atcctttagt tccgccttga tatattcaaa
     4021 ctgtttatat ccgggtgtat ttaacaacag gttgtcgatt ttgtccttac cgcagagatg
     4081 tactacctgg aaatcctcca gaagcttcgg caatgcatcg cggacagcct tgttcacgtt
     4141 agctgcaccc agacttccgc caatgaccat gatgacaggt ttattggcag taaagccgca
     4201 catgttcagt ccggccagcc ggctgccctg tgccagctcc gcacgaatgg gagaaccggt
     4261 caggacagct ttgtcggcag ggatattttt catagtctcc gggaaattgc agcatacctt
     4321 ttctgccacg ggaatgcaca gcttattggc cagtcccggt gtcatatccg attcgtggat
     4381 gatacaggga atatgtaagg aagctgcagc gcggacgacc gggacgctta cgaagccccc
     4441 cttggagaag actacatccg gctggtaatt tttcaggtaa tttctcgctt ccacaaatcc
     4501 cttcatgaca cggaacggat cggagaagtt cttcggatcc aggtatctgc ggaatttgcc
     4561 cgtagaaatg cctgtgtagg gaatgttgaa atccgcaatc agctttttct caatgccttc
     4621 ataagatccc acataggcaa tttcatagcc ggccgcctgc agcgccggta ataaagcaat
     4681 attgggggtt acatgtccgg cagttccgcc gccggtcaat acgatttttt tcataataaa
     4741 atccttcttg tcggtgagat tactgtgcga tcatttcttc cagggcatga gccagctggt
     4801 caggacagga ggtaggcttg aaaccgcagc ggatcccctt gagacggctg attgcttctt
     4861 ccggcttcat tccttttacc agagcactga ttccctgcag attaccgttg catccaccgg
     4921 tgaattttac ggaatcaata acaccatcct ttaattctac gtcgatcgaa gtggaacagg
     4981 taccttttgt cttgtatatc atatgtaaaa ccatcctttt caaactgaat ttctaaggag
     5041 tgattatagc agattttcgg catttataca acaatatgta caaggttttc ttttcacgaa
     5101 aaatgtaaaa gaaatacttt ttctgttcgg caacgtagaa gaatgacgat gaaaaaacgt
     5161 acaaaacaag cctctaatac gttatactct aaggaagcga tagaaaggag cgcaggttat
     5221 gtttacgatt ttcaaagaag aaaaagtgat cacaaccgga attctggtat ttctgggact
     5281 tagtattctg gtgcgtctgc ttctggcagg attttaccat acgatgataa aagagacaga
     5341 taatatggca acgacaaata accgcctgtt gaaacagtgt aaagtaaaat ttgcaaattg
     5401 ttaccagttg aatggtggag tatcaaacat tccggtgttt gtggacaagt tcctcaaccg
     5461 gttggcgttt ggacacctat cctttgatgc ctggtatcat ttgtccggcc agtgcatgct
     5521 gttttccatt gtgtttgccg gtgtcgggat ctgtaagggg atcatgggcg gaaggatgct
     5581 gggggaaatc ctgccttttt atatagtaag cttcttagga atgtatcttt atttttcctt
     5641 gtcagccatg gtggatatca agggaaaacg cagaatatta aagacgaacc tcatagatta
     5701 tctggaaaat catctgtcgg gccggatctc tgtcacggaa caggattatg aaagactcta
     5761 cggaccggga agcctggggg aggagatcgc aaccggcgga aaggcgggaa gaaggcagcg
     5821 tacttccggc agacggacgg tggaactgat gccgatcagc agcagaatgg cgcagccgga
     5881 agaaacggga gatcgtctgg acagagaagt attttccgta gaaaatgccg cacatgaaaa
     5941 gccgggagaa aaagtgctgc cgcagagacc ggaggatccc gccagagtca cggaggaaga
     6001 attacaggca ttgcttcagg aattacttac agtatagcgg aaaatgtcag acgtccgaaa
     6061 gaattcagaa aacattcccc ggatctatag atatgtcttg aaaatgagat gtaactttgt
     6121 taaaataaaa acggattttt taagtaagta acgcaagaat gcgtgatata tctgcaagat
     6181 ataaggagga gcattatgag cagacaagtt acatttgatt attccaaggc cggctctttt
     6241 atttctgaag aagagatcgg ttacatgaag aagcttaccc tggatgcgaa ggagaccctg
     6301 gtatccaaga ccggcgcagg caatgatttc ctgggatgga tcgatcttcc cgtagattac
     6361 gataaggaag agttcgcccg catcaaggca gcagcgaaga agatccagag tgattccgac
     6421 gttctgttgg ttatcggtat cggtggttct tatctgggag ccagagcggc tatcgagttc
     6481 ttaagccaca gcttctacaa tgtactggat aagagtgtac gtaagactcc ggagatctac
     6541 ttctgtggta actccatcag ctccacctat ctgaagcacc tgatggatgt agtaggcgac
     6601 agagatttct ccatcaacat gatctccaag tccggtacca ccacagagcc cgctattgct
     6661 ttccgtgtat tcaaggagaa gctggaagct aaatatggca agaagggcgc ggcagagaga
     6721 atttatgcta ccaccgataa ggcgaaggga agcttaaagc atctgtctga tgaggaaggt
     6781 tacgagacct tcgtagtacc cgatgatgtg ggaggacgtt tctccgtact gaccgctgta
     6841 ggtctgttac cgatcgctgt cagcggtgct gatatcgaca agctgatgga aggtgcagcc
     6901 agcggaagaa agcgtgccct ggagaatgat ttcgaagaga acgatgcttt acagtatgcg
     6961 gctctgcgta acattttact gcgcaagggc aagagtgttg agatcctggc gaactatgag
     7021 cccgctgttc actatgtaag tgagtggtgg aagcagctgt tcggtgagag cgaaggtaag
     7081 gacaacaagg gtctgttccc cgcaagcgtg gacctgacca ccgatctgca ttccatgggt
     7141 cagttcattc aggacggttc ccgtgtaatg tttgagaccg ttatcaatat cgacactccg
     7201 agagaggagc ttacgatcgg cgaggagcct gtagatctgg acggtctgaa ttatctggca
     7261 ggcaagaccg ttgatttcgt caataagagc gctatgaacg gaaccattct ggcacatacc
     7321 gacggtcagg ttcccaactt catggtaacc gttcccgagg taaatgagtt ctatcttggc
     7381 gagctgttct acttcttcga gttcgcatgt ggtgtcagcg gatacctgct gggtgtaaat
     7441 cctttcaacc agcccggtgt agagagctac aagaagaata tgttcgctct gcttggcaag
     7501 cccggttatg aggcacagag agaagaactg ctgaagagat tgtaa
//
LOCUS       DAWDHD010000118         7527 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_574_length_7527_cov_10.976713, whole genome shotgun sequence.
ACCESSION   DAWDHD010000118 DAWDHD010000000
VERSION     DAWDHD010000118.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 7527)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7527)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7527
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_574_length_7527_cov_10.976713"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1823 a         2022 c         1564 g         2118 t
ORIGIN      
        1 atcacatatt gcagctttca ttttccatgc tatatacttt cccatcgttt cccctctcct
       61 ttatagtata attatgccac aactatgtcc tttttcaaat tatttttatt cattaaaagc
      121 atacataaaa acggccttta gacttatgcc aaagaccgtt acttttacaa tattattctt
      181 cattcatctt agccagcttg gctgcctggt cggctgcgat gcaggcgtcg atgatctctt
      241 cgatatctcc gttcatgatc ttgtctaact tgtacagggt caggccgata cgatggtcgg
      301 tgacacggcc ctgggggaag ttgtaggtac ggatcttctc agaacggtct ccggtaccga
      361 tctggctgcg gcgcatctct gcttccgcat cgtgtctctg ctgctgctcg atatcataca
      421 gcttagactt taacaatgcg aatgccttcg ccttgttctg gatctgagac ttctctgtct
      481 ggctgtatac cacaattccg gaaggatagt gggtcagacg tactgcggag tcggtggtgt
      541 tgacacactg accaccgtta ccggatgctc tcatgacatc aatacggata tccttgtcct
      601 cgatgacgat atcaatatca tcgtccacct ccggcataac tgccacactg atggtggagg
      661 tatggatacg tccgccgctc tcggtctccg gcacacgctg tacgcgatgt acgccactct
      721 catacttcat cttggagtaa gcaccctgtc cgctgatagt aaactgtact tccttcatac
      781 cgccgatacc aatctcatcc acgttgatca gctctacctt ccaacgctgc ttctcggcat
      841 aatgcacata catacgatag atctccgctg caaacagtgc agcctcatcg ccgccggcac
      901 cggcacggat ctccacgatt acattcttgt catcattagg atccttgggc agtaacagga
      961 tcttcagttc ctgctccagc tcttccacgc gcttcttgct gtcgtttaac gtctccttga
     1021 tcatctcgcg catttcttcg tctttttcct cttccagcat ggaaagagaa tcttcaatat
     1081 cctgcttgca ctttttgtac tctgtatacg catccacgat aggtgtcaga tcactctgct
     1141 ccttcatcag cttgcggaaa cgttcctggt tattcgtgac cgtgggttca tgcagttcgc
     1201 ccatgatctc ttcaaaacga atcaataaat cttctaactt gtcaaacata actaattcct
     1261 ctcaatataa gttgtaacag ttcagccatg aacatattgg agagcaaaaa tcatgcttgc
     1321 atgattttgt gaacgaatat gttcatgaac taagcgccag gtttatgagc aaaggtagtc
     1381 acgaagtgac ggaaagcgtg tcagcgcgac tttgcgaatg gtgcgggctg aactgttaca
     1441 tacgttccaa aaactacccg gtccagaccg gcataatcct gcaccacttg tacctcgcgg
     1501 tacccttcgg tgcgcatcag ttccgataca gcctgtccct ggtcatatcc gatctcaaaa
     1561 aagagcattc cactgctcac cagatgtttt cctgcttctt cgatgatacg gcggtaaaaa
     1621 tgtaaaccat cctccgtacc gtccagtgcc atcctgggtt catgatcccg tacctccggc
     1681 atcagcttct ccacttccgc actggcgata taaggcggat tcgacacgat gatctcgaat
     1741 tttccctcca ccttgtcaaa caggtcactc tgtaaaaaat gaacccgatc tgcgttcgcc
     1801 gcatccaaaa gcgtctccgc attttgctct gccacttcca gcgcatctgc agacaaatcc
     1861 acacccagtc cttcacaggc attgctgtaa tgcagaaggc ttaacagaat acatccggat
     1921 cccgtacaca tgtcaagcac ccgcattccg tcatggagtt ccttcaatac ttcctccacc
     1981 agaatctccg tatcctgtct cgggatcagt acgtgctcat tgacggcaaa ggtcagtccc
     2041 ataaagtcct gctccccggt cagctgctgc agcggaatgt gctccgcccg ctgtccgatc
     2101 cagccaagat atttctcttc cgcttccggt gtcaccgact gttcgccgtg caccagcaga
     2161 tcattacgat tcgtaccgca taccgcttcc agcagaagtc tcgcatccag tgtggcttct
     2221 tcgattcccg ctgtctgcaa agaaagacag cccttttcat agcattcccg ataggtcatc
     2281 ttaatcctct ggttctgcgg atgcatcgtc cttcatccag ctctcatcca cttcgtaacc
     2341 gaaggtatcc tgtaaatatt tcttccagtc gaacacagct tctacggaag cgatggctac
     2401 ctctgccatg ctctcatcgg gttccctggt ggtcatacgc tggatccaca taccgggagc
     2461 agacaggatc ttaataaaca cattatccgt gcgtccggcc agacggatga tctcgtagga
     2521 aattccggcc acaaccggca tcaacagaat acgaaggatc actttctccg ccacattatc
     2581 cacctggata aagaagaaca gcacaatact taccagcata acaaagaaaa tgaagctggt
     2641 gccgcaacgt ttgtgcagtc tggagctgcg catcacattg tgcaccgtaa ggggtctgcc
     2701 cttctcgata cagttgatgc atttgtgctc cgccccatga tactggtaca gcctgcggat
     2761 atccttcatg gcgctgatac cccatacata cagcaaaaac agagcaatac ggatcacacc
     2821 ctcaataatc gccatcaggg agcggttgcg gataaagctg tcaaacagag aggaaatgaa
     2881 atacggcaat acgataaaga tgcctactgc caacataatg gagatcaccg tcacaatacc
     2941 ggacagcagc ttttctccgt tgccaccgct catcttgtcc attgccttat cgaatttcgt
     3001 ttctttgcct tcctcttctt catagaagga agcggaataa ttcaatgctc tggttcccag
     3061 gatcagggaa tccaggaaat taaagatacc acggataaag ggaagctcct tgaacttact
     3121 tccgtgagcc agaccctgat aggtctccac ttccacttca atctcgccgt ccggcttgcg
     3181 gaccgctacc gcatacttct ctttattttt catcataacg ccttccagca cggcctgtcc
     3241 gccgataccg gaataacgac tctttcttct agccataaaa cctcatcctt ataaatgcag
     3301 tattaaatag gaacacagtt tcctatacga caagaaaagg ttgagataag aatacctcaa
     3361 cctttcgctt gcaatcttat ttaacaccgt atttcttatt gaacttatca atacggccat
     3421 cggttcttgc agacttctgc tggccagtgt agaatgaatg acacttggag caaacttcta
     3481 cgtgaagctc gggcttggtg gagccggtta caaatgtgtt accacagttg caggttacag
     3541 ttgcctggta gtaagcggga tggattcctt ctttcatttc tttcacctct ctataaaaat
     3601 gtcgtgtata agttcttgtt ctcatcctca ctgcttatgg aaccacaaac agcggtatta
     3661 ttgtaccata agagtttaca cgacgcaaga cctattttta catatttctc ccctgatatt
     3721 gcactgtaat gacataaact gttttttcag tctcatcaat cttataaatt gccatataat
     3781 tatcgaccag aagctgctta tacccttttc cggcatactt gccttctaat ctatcctgat
     3841 gtgaaccggg aaaagtctcc agttttttta tcgctctcca aattctgtct gtttgtgctt
     3901 ttgcattttc cggaacctgt ttttctatgg cgatatacct gtagatttct tccatatcac
     3961 gatatgctct cggatacaac tttatatggt atttaaccat aatatttttt gtctagcctt
     4021 tccattgcga catccatatc catcgtatcc gcacccgcag caacttcctc ttccgcctca
     4081 cagatggcct ggtcgatttt atgaattcgt gcaaaacgct cataaagttc tgcgctcatc
     4141 actaccagat cgctgtagcc attcttagta ataaaaatcg gttcctgctt tgtatgtgct
     4201 aattcggata tttcacttgt ttttctcaaa tctctgattg gcatgatcaa tggcataata
     4261 gcacctcctt tatgtcataa ttgtagcaca attatgacac atcgtaaagg tatatattct
     4321 gccatcattt ccaatttttg tcattttcta tctcttgcct agataaaccg gttcttcttc
     4381 accatattca caaattccac attattgcgg gtatgggaga acatatcaag gatgcggtcg
     4441 gtagcttcgt cgggctttaa gccattcagg gccttacgca tgatattgat ggcttccagt
     4501 tcttccggtg tcaacagcag atcctctctt ctggtgccgg attttgccag atcgatagcc
     4561 gggaaaatac gtttctcgga cagctttctg tccagaacca tttccatatt accggttccc
     4621 ttgaattcct cgtataccac atcatccatc ttgcttccgg tctctaccag tgcggtggcc
     4681 agaatggtca ggcttcctcc ctcacgcata ttacgggctg caccgaagaa tctcttgggc
     4741 atgtggagtg cggcgggatc gagaccaccg gacagagtac gtccgctggg gggaaccacc
     4801 aggttgtacg ctctggtcag acgggtgata ctgtcaagca ggatcacgac atccttctta
     4861 tgttctacga gacgctttgc acgctcgatc accatctctg atactctctt gtggtgctcg
     4921 ggaagctcat cgaaggtgga atagattact tccacattgt ctccgtggat tgcttcctta
     4981 atatcggtaa cttcctcggg acgctcatcg atcagtagga tcagcatgtg catcttgggg
     5041 ttaccgttca aaatagactt tgccacttcc ttcaacagtg tggtcttacc agccttgggc
     5101 ggggatacga tcataccacg ctgtccctta ccgacagggc ttaccagatc catgactctc
     5161 atagccacgc tacagccggg agtctccatc ttgatgcgct catccgggaa gatcggcgtc
     5221 atatcctcga atgccattct cttggctgac tcttccactg tataaccgtt gatgctcttg
     5281 acgaacagca gtgcgctgaa cttctcctgc tgggtcttga ttctcttgtt gcccttcagg
     5341 atatcaccgg tcttcagtcc gaaacggcgg atctggctgg gagccacata cacatcgttc
     5401 tcaccgggca tataattctc gcaacggata aatccgtaac catccggcat aacctctaag
     5461 ataccactgg cttcgatacc gctgtccagt tccttgggat actggctctc atcgtatgcc
     5521 acgttattcg gattgaaggt acgctctctg gtgtaagctt cacttcttac gccgactgtc
     5581 tctgtaccgg tttcggaacg gtcagtgcgt tctgcacgat ccgtacgctc ggtacggtct
     5641 gtccgatagt ttctgtcatt tctatcctgt ctatcatatc tgtcctgtct gtaattcctc
     5701 tcctgtctga cagggcgttc tgttctctcc ggcttctctg cctcttcgct cttagcagcc
     5761 tcctgttttt cttccgctgc cttagcttct tcctcttttt tcaggcgctc atcttctgca
     5821 agcatagctt ctaccagatc tgcttttttc attgtggaag ttcccttcag gccacgtact
     5881 tttgccagct cctttaactg aactaatgca agtgattcat atttttctct catataccct
     5941 cctatggaaa taagatttac tataaaactg ttaactaagg ggaattacat ccgatttaca
     6001 ttcgacatgt cagttgaata aaaagatatt aaaactgcat ctgaagccat tataatacat
     6061 ttctttccaa ataggaagtc ccaaaataaa aattttattt tttgtaaaag tggtgctaaa
     6121 tccttgcgaa gatatcgatt ctattatatc attatgaata gcgtccggat ttcgtatccg
     6181 gcaaatcatc catgattata tgaagtaatg accaaacaaa aaataagaga ggtattgtca
     6241 tgacaaccaa tgaaaaagcg ttactgatgc atgaaaaatg gaacggtaaa ctggagaccg
     6301 tgtccaagac tcccgtcaag acccgtgagg atctggctat tgcttatact cccggtgttg
     6361 cggagccctg caaagtaatc gcccaggaca aggaagctgc ttacaaatac accatgaagg
     6421 ccaacaccgt agccgttgtc tccgatggaa gcgcggttct tggtcttggc aacatcggtc
     6481 cctacgcagc catgcccgtt atggaaggca aggcagtcct cttcaaagaa ttcggtaatg
     6541 taaatgctgt ccccatttgt ctggacaccc aggataccga agagatcatc acggctgcgc
     6601 ccaatctggc tcccggcgtc ggcggcatca atctggaaga catcagtgct ccccgttgtt
     6661 tcgaaattga ggaacgtctg aaggagattc tggacatccc cgtattccat gatgaccagc
     6721 acggtactgc catcgtcgtt ctggccggtg tcatcaacgc cctgaaggta gtcggtaaga
     6781 aaaaggaaga ctgccgcgta gtggtaaacg gtgccggaag cgccggggtg gctatcacca
     6841 aactgcttct gacctacggt ttccccaata tcatcatgtg tgacaaggta ggtattgtct
     6901 ccaaagatac cgaaggcctg aactggatgc agaaaaaaat gacagaagta accaatttaa
     6961 acaacgagac cggatctctg gccgatgctc tgaagggtgc tgatattttc atcggtgtat
     7021 ccgctcccaa cattgtcaca ccggaaatgg tagcttccat gaaccgcgat tccatcctgt
     7081 tcgccatggc caatcccgtg ccagagatca tgccggatgt agccaaagcc gcaggtgccc
     7141 gtgtcgtagg aaccggacgc agtgattttc ccaatcaggt gaacaatgtg gtcgctttcc
     7201 ccggtatctt caagggtgct ctggaaggtc gtgctaccca gatcaccgaa gagatgaaac
     7261 tggccgctgc cgaagccatt gccggtctgg tacccgcagc caagctctcc gatgacaata
     7321 tcatgccgga agcatttgat ccccaggtag ccgaagttgt ggcaaacgct gtaaagagcc
     7381 acatccgcta atgtgttcgg acagcattgt gagagactgg cacggcaagc cctactactc
     7441 tctggattcc tattgcaaaa atacgtttca ccataaatgc tataaaatag ccctgaatgc
     7501 ccggatgtcc tgtcccaatc gggacgg
//
LOCUS       DAWDHD010000119         7482 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_578_length_7482_cov_14.899017, whole genome shotgun sequence.
ACCESSION   DAWDHD010000119 DAWDHD010000000
VERSION     DAWDHD010000119.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 7482)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7482)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7482
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_578_length_7482_cov_14.899017"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1979 a         1741 c         1917 g         1845 t
ORIGIN      
        1 tatcgcatgc tccgggagtt cgtggatttt gccaatgtat ttaactaact ttccatttac
       61 ttccggtgct tattctccaa agccgcttcc acgaatccct taaataacgg atggggtctg
      121 ttgggtctgg acttgagctc ggggtgtccc tgggtggcta cgaagaaggg gtgctcggta
      181 agctcgcaca tttccacgat acgtccgtca ggggacagac cggacagacg aagaccgtgt
      241 tcggtgagga cggcacgata atcgttgttg acctcataac gatgacgatg tctctcggag
      301 atattctcct gtccgtagag ctgataagca cgggaatcct tatccagtac acaagggtat
      361 gcacccagtc ttaaggtacc gccgatatcc tcaacaccgt tctggtccgg catcagagcg
      421 atcacaggat gtgtggtgtt aggatccagt tccacactgt gggcatccgt atacccgatg
      481 acatttcttg catattccac gattgtcagc tgcattccca gacacagtcc caggaaggga
      541 atcccatggg ttcttgcata ctggatcgcc aggatcttgc cttcgatacc ccgggaaccg
      601 aagccgccgg gtaccaggac accgttgcag tcacctaaca gttcttccac attttcaggc
      661 gttaccgtct cggagtcgat ccatttgatg tgtacggtcg catgattggt gataccgcca
      721 tgcttcagag cctccacgac actgatatag gcatcatgca gagagacata ttttccgacc
      781 agagcgatct gcacttcatc ggtgggatga cgcagatcct ctaccatctt cttccagtct
      841 gccagatccg gttccgaaca atccagatgc agacattcac aggctacctg tgccagatgc
      901 tctttttcca tagctaacgg agcctcatac agatattcca catccagatt ctgcaataca
      961 tgactctgag gtacgttaca gaacagagca atcttgtcct tgatggactg atccagagga
     1021 tgctcacttc tgcagacgat gatatccggc tggatgccca tgccctgcag atccttaaca
     1081 ctggcctggg taggtttggt cttcagctcc tgagaagcac tcagataagg aatcagcgtt
     1141 acatggatca gaatggcatt atcatggccc acctcatgct ggaactgacg aatggattcc
     1201 aggaaaggct ggctttcgat gtcacctacg gtaccgccga cctcaatgat ggcgatacgg
     1261 gtctcgtcat ccgtaaaatt gcggtagaaa cggctcttga tctcattggt gatatgaggg
     1321 atgacctgta ccgtacctcc gccgtaatca ccacgacgct ctttctgcaa tacggaccag
     1381 tataccttac cggtggtgac attggaattc ttatccaggt tctcatcgat gaaacgctca
     1441 taatgtccca gatccagatc ggtctctgta ccatcttctg tgacgaatac ctcgccatgc
     1501 tggatcgggt tcatggtgcc gggatcaata ttgatataag gatcgaattt ctgcatggtt
     1561 accttataac ctcttgcctt taacagtctt cccagggaag ctgcggtaat tccttttccg
     1621 aggccggata cgactcctcc ggtcacaaat acgtacttta ctgccataat tttctcctcc
     1681 ttttgatata gtaactattc agtctccatt cgcaaaatcg ctctttcaga gctttcctga
     1741 ataacatgct tgcatgttat ttgctcatgg tggttacttc gcttttttaa tagctatgca
     1801 tataaaaaga gcgaagaact ccccttcggg agcaccttcg ctcaataact gccataattt
     1861 atagtagcat gacaaaatgt ttagttgtga taaagcttct ggttctggta atgaggttcg
     1921 catgtcagat tcacacctaa tttgcggaag gtattcatgt caatctggga caggatcacg
     1981 ctggagtgca cttcggaacc tttcaggttc ttcaactgct ccacagcagc cttcgcggta
     2041 tcatccgaag ctgcacagat ggaaagggcg atcagcgtct catctgtatg aagtctggga
     2101 ttgcggttcc ccagatagtt cgtcttcaga tcctggatgg gaccgatggc ggtaggtgag
     2161 atcagctgta cttcgtccgg aatgcctgcc agagctttca gggcatttaa taataatgcg
     2221 gaagaagcac ccagcagagt actggtccgt ccggtgagga ctctgccgtc cggaagctgg
     2281 atcgcagctg ccggttcccc ggtggtctct gctttaatat tggctgcgct gactactgca
     2341 cgatcgtcga tggtgacacc tgcctgcttc atcagaagct caagctttaa gatctggtcg
     2401 tcactggcgg taccctggcg cttctggcac agagtgtggt agtagcggcg gatgatttcc
     2461 tgtttggcgg cgtcgcggac agcttcgtca tcaacaatac cgaagcctgc catattgaca
     2521 cccatgtcag taggagactt gtaagggcag ctgcccatga tcttctcgaa catagcactg
     2581 actacgggga agatctccac atcgcgattg taattgatcg tggtctcacc ataagcttcc
     2641 aggtggaagg gatcgatcat attgacatcg ttcaagtctg cggtagcggc ttcataagcc
     2701 aggtttaccg gatgctttaa cggaatattc cagattggaa aggtctcata tttggcataa
     2761 ccggccttga tgcctctctt gtattcatga tacagctggg agagacaggt agccatcttg
     2821 ccgcttccgg gtccgggagc agtcaccacc accagagaac gggtggtttc aatataatcg
     2881 ttcttaccgt aaccttcatc acttacgatg aacggaatat tggaaggata tccgggaatg
     2941 gaataatgac gatacacctt cattcccagg gactccagtt ttttctgata agcaatggca
     3001 ctgggctgtc cggcaaagcg ggtcagacac acactgccta catacaggcc gtagccgcgg
     3061 aatgcatcga tcagacgtaa cacgtccaga tcataggtga tgcctaagtc ggaacgaacc
     3121 ttgtttttct caatgtctgc tgcattgatg acgatgacga tctcagcctc gtccttcagc
     3181 tgtgccagca tgttgatctt actgtccgga tggaaaccgg gcaatacgcg ggatgcatga
     3241 taatcgtcga acagcttgcc gccgaactcc agatacagct taccgccgaa ttttgcgata
     3301 cgctccttga tcttctcaga ctgcatgctg aggtatttgt cattgtcaaa acctatcttg
     3361 tacattgctg acacctttct ctgcgaatct ttgcaactaa aatcccatga tgttctatta
     3421 tagtttagtg tttcccaaaa atctactgga aagtaaaatt ttttttggaa atcattatta
     3481 tatctaacaa ttttaagcaa ataatcacat aatgagcaat tttcaacaaa gagatccttt
     3541 tttataatga aattgtaact attcagaacc ccattcgcaa aaccgctact tcgtagcttc
     3601 cttttgctca tgtggttact tcgccatata aaactgctta tctgcctgaa tgcaagcatt
     3661 cgcagaacgc agttttgcat ggctctgaat agttacatga aattacaata aatacagaac
     3721 aaaaaagttc tgaaaaagca aaaaactata tacgggaggg acgcgttttg caatacggct
     3781 atttcgataa tgaaaaaaga gaatacgtca tcgacaacgt ggctctgccc tgttcctaga
     3841 cgaactatct gggtgtcgag gacatggcgg cggtcatcaa ccatacggcg ggaggttatc
     3901 tgttttacaa aacaccggag tatcacagga tcagccggtt ccgtgggaat ggggtaccca
     3961 tggaccgccc gggattctat gtatatatca gggataatga aaagaaggat taccattcca
     4021 tctcctggca gccggtagcc aaggatttat ccaaggcaaa ataccgttgc cgccacggac
     4081 tgtcttacac cgtctacgag agcgaatatg acggactggc atcctcacag accatggtta
     4141 ttccccgcgg ggaaaatgta ctgttgtggg atgtaaaggt gaaaaatacc accgatgcag
     4201 ttagaaatct ttccttattt acttacatgg aattttcgtt ccatcacatt atgatagaca
     4261 accagaattt tcagatgagt ctgtattgtg ccggttcttc ctatgaagac ggcatcatcg
     4321 aggaggactt gttctacgaa gaaaaagggt atcagtatct taccgccaac ttcacaccgg
     4381 acggctatga ctgcgtcaga gagaaattcc tgggagttta tggtaccgaa gatcattcgg
     4441 caggcctgga gagaggaact ctggaaggtt ccacagaact tggtggcaat caccgcggca
     4501 gcttgcagaa gaacttcaaa ttacagcccg gcgaagaagc ccgttttgtg atcatgctgg
     4561 gcgagggcaa tcgtgaagag ggcagacgga tccgggtaaa atacagcgat ctgaaaagag
     4621 tggacgcagt ttacaccgat ctggcagcct actggaaaca gaaatacgct gcactgcaga
     4681 tccagacacc caacgaaggt atgaataccc tgatcaatac ctggactttg tatcaatcgg
     4741 agatcaacgt tatgtttgaa gggcgttagt atataattgg ttgttgtgag ttgtttgttg
     4801 ctggcgaatg cccggaaact gtagaaaaac atggtttccg ggtattcgtt atttccggga
     4861 atagttcaga gttttttcat atttgagcta ttgatttatc cacaggttat ctctataata
     4921 gatatgattg ctcagatttc aactgtgcag ttgaaataac ggaagtgcaa tagcgtataa
     4981 aatagaaaat ggagatgaca tgagaaaatt aattgagtca tataataacg gctatggccc
     5041 cggcaacgga ggcaatggca acggagctcc cggcagcggt aacaatggcg ggaacggcaa
     5101 taacagtaac ggaggcaacg gcaatggttc accgaagcgc cccagcatcc tgatgctgat
     5161 cctgtcggga ctgatgacag tgatcgttgt actgatgctg tataatgcgt tcttcggcac
     5221 caataccgga acggaagtat cttataccga tttcctgaag gatctggaag cggacaaggt
     5281 tgctgcggtg gaggtaaata aggaaagcgg catggtgacg gtacagctga agcaggaagc
     5341 tgcagccacc ccggcaccgg gcagttatgt cagcttctat ggaattaccc agaaggtgac
     5401 acagtcccag actgtatatt atacaatgct gatggaggat ctggataccc tgacagccag
     5461 attgcaggag catgatgtac aggggaatcg cgtgaccagc aatacttcca atttcctgat
     5521 ggagattctg gtaaccgtgg tattgccggt ggtactgatc tggatcgtgg caggttttct
     5581 gttccgcaga atgggcggaa gcggcggacc catgggcgtg ggcaagagca atgccaaggt
     5641 atatgtacag aaggagaccg gcgttacatt caaggatgtg gccggtgaag acgaagcaaa
     5701 ggaatctctg gtggaagtcg tggacttcct gcataatcca cagaaatatt ccaagatcgg
     5761 tgcaaaactg cccaagggag cattgctggt gggccctccc ggaaccggta agactttgct
     5821 ggccaaggca gtggcaggag aagcacatgt accgttcttc tctctgacgg gttctgattt
     5881 tattgagctg tatgtaggtg tgggcgccag ccgtgtccgt gacctgttca aggaagcgac
     5941 caagaacgcg ccctgtatcg tatttattga tgagatcgat gccatcggac gaagccgtga
     6001 ttccaagtac ggtggaggca atgaagagag agagcagacg ctgaatcagt tattatccga
     6061 aatggatggc ttcgacagtt ccaaaggtat cctgatcctg ggggcaacga atcgtccgga
     6121 gatcctggac aaggcgctct tacgtcccgg acgttttgac cgccgtatca tcgtagacaa
     6181 gccggatctc aagggccgtg tggaaatcct gaaagtacat tccaaggatg tgaagatgga
     6241 cgagaccgta gatctggatg cgattgcact ggctaccagc ggagcggtcg gttccgatct
     6301 ggccaatatg atcaacgagg cagctatcaa tgctgtcaag gaaggcagag aatttgtaag
     6361 ccaaaaggat ctgttcgcgg ctgtggaaca ggtgctggta ggtaaggaaa agaaagaccg
     6421 catcatgagc aaagaggaac gtcgcatcgt atcctatcat gaagtgggac atgcactgat
     6481 cagcgcttta cagaaaaact ccgagccggt acagaaaatt accattgtcc cccgtaccat
     6541 gggtgcgttg ggctatgtga tgcaggtgcc ggaggaggaa aaatacctca ataccgaggc
     6601 agaacttcgg gatatgttgg taggtctggt gggtggacgt gccgctgagg aagtagtatt
     6661 cgacactgtg accaccggtg cagccaatga tattgagaag gctacatcca ttgcccgcaa
     6721 catgatcacc cgctacggta tgagcaaacg tttcggtctt gtgggtctgg ctaccgtaga
     6781 gagccaatat ctggagggaa gaaccgcgct gaactgtagt gatgaaacgg cagcagccat
     6841 tgacgaagaa gtcgttgcca tgatcaagga gagctacgat caggcattgc agatgcttcg
     6901 cgagaataga gaactgatgg ataagctggc agcgttcctg atcgagcgtg agaccatcac
     6961 cggtaaggaa ttcatggaga tctttcgcag agagaaggga ctgcctgacc cagaggaaga
     7021 gaagaaagat gtgacagaaa caaatgctgc gggcagtgca gtaaagggca acagcgtgga
     7081 tgtgaccatt ggggaacagg tcatgacttc caccgattca actttccaac ccctgacttt
     7141 cgaagacagc ctgcaggaag caaaggagca gactgcaccg gcgcaggata ccgcaaagca
     7201 gcctgcagaa tccgcaaaag aagagacttc cgcacaggag aagcatgaac aggaatccac
     7261 agagcagaaa agcgatgctg ccgaggagtc gaaggatcct aaggatcctc ctcagggacc
     7321 ggtaggactg ttctcccatg gaacattata gatgtcgaga acttttgaac ccgacaccta
     7381 taaaatagag taattagcaa agagcattag tcaaatgact gatgctccag actgtagaca
     7441 aagctccttc ctgtgaggga gcttttcttg tgtcatgact gc
//
LOCUS       DAWDHD010000120         7434 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_586_length_7434_cov_12.628134, whole genome shotgun sequence.
ACCESSION   DAWDHD010000120 DAWDHD010000000
VERSION     DAWDHD010000120.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 7434)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7434)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7434
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_586_length_7434_cov_12.628134"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1824 a         1897 c         1425 g         2288 t
ORIGIN      
        1 ctacatgcat atctactgca ctgatctgat ggttttcata agtattgtaa taataaatac
       61 cttttgtaac attacaacac gacgtataca atgtgatttc atattttcca tccgctactt
      121 cgcaacatcc tcgttgctga tccacagatc cgagaatgtg gaagaattgg ctaacgcttt
      181 cttcctctga ttcacctgaa attgcattta cctttgtaaa agctacacgt acaaagcgtg
      241 aggaagatga gagatctccc ggaagaccta atcctcccat acctctacta tatgcatcaa
      301 gagccaaatt ttcgcagaaa gtatttctgg gctgtttagg agataaatac atataattat
      361 ttagttgaaa catctgctgt ggaaatggcg gattattcgt aagcactcct actggattgt
      421 cataaatatg caaaccatct gacatagatt ctaccgtaat tgactcattc tcatcagaaa
      481 tgatccagtg taattgtgct aatgggaatt gctcgctgaa aggagtatta acaatattaa
      541 ttcgttcaag aagctcgcga acttcaacta aagaagaaca ctgacttaaa atccacggaa
      601 taaattcaaa ctgtgcaata ttttccacat ctggcttaat cgcggcataa acagcatttc
      661 cgacaaaatt cagtcctgcc attgccactc ctttttcatt catagcatca taatacaaag
      721 gataatcctc tgcaacatga gccattccaa taattgcata atgatttttc atatcgccaa
      781 catggcgaaa attaaacgca taattacgtg gtgtaattac tatctgatca ccataagaga
      841 attcataatc gagggttcgt ccaaagtaaa aatcttttgt tttataagtt gctgctgtac
      901 acatgatcgt ttatcctcct aaatagattg aaatatgtca atgagttttt ttcttgaaca
      961 atagtatagc acaatcttta ttttgttcca acttcataca tgatcataac atgtaaactt
     1021 taaactattt tcctagatta tttctccatc agtttttcaa accaggtatc catagctaag
     1081 atgaattgac ctacgtctgg caattcttgc agcatttcgt ttctcgtata tatattttac
     1141 cacatacttt ttccaaaggc actgacttct cagcccttaa ttttcatctc tgctattata
     1201 cgttcttccc tctgtctctc ttcacaataa tcccagaagt ctttcaatgc ttcctcatcc
     1261 gatttccacg gcaatggtat gggatccaat tcatctaatc gcttcacaag tttgcgtacc
     1321 tcttcggtat cgaactcctc atcacttgcc tccattacat aaaaatccag tttttcattt
     1381 atcattctga tttcttcgtc tttagtcatg ttaaaacctc actacattat atacgtgaca
     1441 gttttcgaca aaatcttata aattttttga aattattttc tattattttc tattccagac
     1501 tgcttcggat gcacaaacct cagatgcaca aaagatcccg ggctgctcac ccgggatcct
     1561 ttgaaaagag atgtatgaaa ctatgcgttg catttgttcc tgtcgatcca taatgtaagc
     1621 tcttacattt tgaatcataa ttattctacc attatttcga ctatttggca aacagatttc
     1681 ttacagttgc gagtttttcg cacttttagc caaaatcagg acttcttttt tgtctaatcc
     1741 tcatatttaa agtagtcaaa atccgctgct ttgcggcttc cacagcctcc gttgttggca
     1801 tagatgccta ccagcgtacc ggtatggcgt ttaggatcat cgggaacacc ctcgtcacag
     1861 aggtaaatgc agttctccag aactccgacc tgctcccagt tctctccgtc atagctgtaa
     1921 tagaaagttc tggtcagctt ctccaccact actttcagat acagagcgcc ttccttcaca
     1981 tggtccactt ccgccaccag ctcctcgccg tggttgcggt tgatgaccag ttggatcttc
     2041 cgtccgccct catagcacag agagcatctg gcataggtgg ctgtactgta gtagcaggtc
     2101 agtcccgcct gttctccgtc cttatccggg tagaattcca gcttcgtctc tgccacgtag
     2161 gacaactcct gttctctgcg taacaacgta tttttcgcac ggatctcatt caactgtccg
     2221 tctctggtcc agatcctgta atacccggga cgttccgtca gtgaccagga tccgttatcc
     2281 gggttgcgca cgaactccca ttccagattc agctttgtat cgttaaaatc gtcgaacaga
     2341 tttctctcat aaatacactc cggaagatcc ggtgcaatct gtgtcaggct ggggcctttt
     2401 ccttcgttga cggtaaacca gccatcctcc gtccactgta cgggatccag tgccgactct
     2461 ctgcctacgg ttgtataatt gccctcgttg ggtcttccgc agagataata gcaccaccac
     2521 tgaccgttct gatcctgtac cagcttgccg tgtccggctc tctgaatggg ggctgtggga
     2581 tccttctgac gcatgaccgg attgtaagga gaacattcat aggggccata gaaattctta
     2641 cttcttccca cattgattcc atgaccgtag ccggtaccac cctcagccac gatagcgtaa
     2701 tagtaaccgt cttttttcat gatatggggg ccttcggaac agcgctcgcc ggtgccttcc
     2761 catgcagtct tcgcaggacc agccaccgcc agtccatccg cagtcagagg aaccgcctgt
     2821 gccgccttgg ctatgatcat atatctgcta ccgtcatcat ccacaaaaag tgaggggtcg
     2881 atatcatcca cttccagaca taccggcttg ctgtagggtc cttccggctt gtcagatacc
     2941 atcaccagct gtctgcgcat cacgttattg tcacgcttgc catccgcatt cagacgcaag
     3001 gttacataga tatagtattt tccgtccaca tattcgatat ccggtgccca gattccgtgg
     3061 gaatcccgga tgtcactgat gtccagccat tccgggttcg tgatggcatg gccgatgata
     3121 tgccagtgca ccatatcccg ggaatgggaa atggggattg ccggaaaata ctggaagctg
     3181 gaattcacca tataataatc gtcaccgaca cggatcaccg aaggatccgg gaaaaaaccg
     3241 cgttgtacag ggttatgata agttctaggt tcactcatag ggagtctcct ttttacatgt
     3301 attagtcttt ctatttgcgc aaagttgcgt gtaccacgct catactataa gtgtattctt
     3361 taccaactct cctgtcgtgc cagtttccct tttattttat gacaaatatt gcgttttctc
     3421 cggacatttc aaaatttggc ataattattc cataatggaa tactgcatcc actcgtataa
     3481 cagcttcgtg ccctccatga tctcgggcgg aagcagcgcg cgggccacca gctttaattc
     3541 atcggtttcg atgtctgttc tgcgaagatg ggcataatcg ccgtcaatgc tttctacgat
     3601 gtattcaaaa cgattcatag gggtatacct ctttctttga tgtgtttgag ataagtttaa
     3661 gaggtttttg ggggagagtc aaggggacgc cgccttatct tcgaaaatcg ctctgctttg
     3721 cagagcacgt gcttcgcact tatgattttc tcgataaaca cccataaaaa acaaatgcca
     3781 cacccgaagg tgtggcgctt gttttttcta tgggtgttta catcattccg cccatgcctg
     3841 cgccgccggc gggcattgcg ggggtgtctt ctttgatatt agctacaacg ctttcggtag
     3901 taagtagtgt gcttgctacg ctggtagcgt tctgcagggc gcttctggta accttagcgg
     3961 gatccaggat gcctgcactt accatatcta catattcctc tttcagagca tcgaaaccga
     4021 caccgacctc agactcacgt accttgttga tgataacgct gccttccagt cctgcgtttg
     4081 ccgcgatgtg atacagaggt gcttccagtg ctttcaggac aatgcctgca ccggtcttct
     4141 catcaccttc cagacttgct gccagtttgg caacttcctt ggatgcatga atgtatgcgg
     4201 aaccaccacc acagatgata ccttcctcta cggctgctct ggtagctgcc agagcatctt
     4261 ccagacgaag cttagcttcc ttcatctcgg tctcggtagc agcacctaca cggataactg
     4321 caacaccacc ggacagctta gccagtctct cctgtaattt ctctctgtcg aaatcagaag
     4381 tggtctcttc gatctgcttc ttgatctgtg cgattctgga ctgtaatgct tccttatcgc
     4441 cttcaccgtc tacgatcacg gtgttctctt tctgtacctt aacggatttt gcacgaccca
     4501 gcatatccat tgtggtatct ttcagttcat aaccaagctc ggaactgatc acggtaccac
     4561 cggtcaggat tgcgatatcc tccagcatag cttttcttct gtcaccatag ccgggagcct
     4621 taacagctac tacgttgaaa gtaccacgca gcttgttaac gatcagggtg gtcagtgcct
     4681 cgccctctac atcctcagcg atgatcagca gcttagcgcc ggactgaacg atctgctcta
     4741 acagaggcag gatctcctga atgttggaga tcttcttgtc agtgatcagg atataagggt
     4801 tatccagaac agcttccatc ttatccatat cggttgccat gtaagcggaa atataacctc
     4861 tgtcgaactg cataccttct accagatcca gctcggtctg catggtcttg gactcttcaa
     4921 tggtgataac accgttgttg cttaccttct ccatagcatc tgcaaccagc tgtcctacag
     4981 cctcatctcc tgcggagata gctgctactc ttgcgatatg ctcttttccg tttactttct
     5041 ggctcatctt accgatggct tctactgcac atgcggttgc tttcttcata ccttttctca
     5101 ggatgatggg attagcacct gctgccaggt tcttcatacc tgcatttacc atagcctgtg
     5161 ccagaacggt tgcggtagtg gtaccatcac cggctacatc attggtcttg gtagctactt
     5221 cctttaccag ctgtgcaccc atgttctcga atgcgtcttc caactcgatc tcttttgcaa
     5281 tggtaacacc atcgttggtg atcaggggtg caccgaagga cttatccagt actacgtttc
     5341 ttcctttggg tcctaaggtt actcttacgg tatctgccag ctggttgaca ccagcctcta
     5401 atgctgcacg ggcttctgcg ccgtatttga tctcttttgc cataataatc agcctccaat
     5461 ttatttgttc ttagtcttaa tttgtttttg attttttgca attactggca gctattcaga
     5521 actccattcg cagaacacag ttttgcatgg ctctgaatag ttacattact gaatgattgc
     5581 aaggatgtcg ctctgcttta cgatgatgta ctcttcatca tccagcttta cttcggttcc
     5641 ggagtatttg gaatagatga cctgatcacc aaccttaact tccatcttta cttccttacc
     5701 gtctacgaca ccgccggggc cgacagcaac aacttctgcc tgctggggct tttccttatt
     5761 ctgaccgggg agcacgatac cggacttggt agtctcctct gcaactaact gttttaatac
     5821 tactctgtca cctaagggtt tcaaattcat gacaactgcc tccttataac tatttaaata
     5881 tcatattact ctttttcatt tgttagcact catttttatc gagtgctaat ttctatagct
     5941 tatattagtt ttatattccg agtttttcaa acgcattcac atttgtttaa gaatctattt
     6001 ctcatattat ctcgtgtttt ctctattttt cttttgtttt tatatcacat gtatgaaaat
     6061 gagatttaat atttcgttta taattctaaa aatacatgcc cgtacattcc cactcttaac
     6121 agctcaatga ggtacatgca ccacgtacgg cttcttgtac aagcacaaat cttgtatctg
     6181 acgcatcgta taagcaaaaa gcagccgggg atcctgtcat atcccaagct gctcctgttc
     6241 tactctgtat ttactttgtg atccgcccgt cctatgctgt cttggcatcc tcatgccatt
     6301 tctgccactt cttcttatag ataaagtaac aggggattcc tacgaatacc attccgccca
     6361 agatattgcc gatggtaacg ggaataaaat tattcaggct gtgcaacacc gtcagtcccc
     6421 ggatctgctc tgcggtcagt ccgtaagctt cctgcgcctt tgccacgtaa acaggattct
     6481 gtgcagccat catgcctacg ggaatgtaaa acatatttgc aacacaatgc tcgaagccgc
     6541 cgaccacaaa cgccataata gggaaaaaga ttgcccagac tttgcctacg atatcggttg
     6601 ctgccaccgc catcaggatt gcgatacata ccagaatatt gcacaggata ccggaggtga
     6661 ttcccctcag cggagagata cctaccttgc cgactgccac tttgatagca taggcaccca
     6721 gcaacccacc ggtgtaatcc agatttccgg aaaaataaat caatgtgtca atgatcagtg
     6781 ctcccatcag attgctgaaa tatactacga tcagattccg gatacattca aaccaggtga
     6841 ctttcttgcc taccacatcc atgacgatca gacagttacc tgtaaaaagc tctccgccta
     6901 caaatacaat gatcatcagt cccaccggga agataatacc tgccacaaat cgtgccagac
     6961 ctacattgtc gatggcatgc gctgcggtac tgctggtagc tccacccagt gcgataaatg
     7021 cacccgccat aatgcctaac agaatcatct tgcccatggg aagatgtgcc ttgccctctc
     7081 cggctttcat gttggtatca atgacttccg ccggggtatt aaaatgctgt tccatatttt
     7141 ccctcatttc cgtacagctt cgcctgccgt accttttcct actcattttg cttctatatt
     7201 acactaattc tgcatctgta agcaaggttt tcgcacgatg tattccatta tatacattct
     7261 tttgtggctg tatttcccac ttttgtcatt tcaagtctaa ttttgcaatt tttttggctt
     7321 tttttgcaaa aaagtgctag acaaaagtcg aattgtgtac tatactgaac ttgttcggcg
     7381 agagccgaac cgcttatata agtagatttc cggaagaatt ccccttttac acaa
//
LOCUS       DAWDHD010000121         7424 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_587_length_7424_cov_9.341023, whole genome shotgun sequence.
ACCESSION   DAWDHD010000121 DAWDHD010000000
VERSION     DAWDHD010000121.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 7424)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7424)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7424
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_587_length_7424_cov_9.341023"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2179 a         1494 c         2085 g         1666 t
ORIGIN      
        1 gtattatggt aatggacggc cagggcggag gcgtaggcag aagcctgctg gaggctctga
       61 aggaacggtt tccggaggcg gaactgatcg cagtgggaac caacgccaca gctacggcga
      121 atatgatgaa gagcggcgtg acaagcggtg ccaccgggga gaatgccgtg gtatataaca
      181 gtaagagagc agacgtgatc gtaggaccgg tgggaatcgt catggcggat gccatgttag
      241 gtgagatcac ccccaccatg gcagcagcgg tagcttcgaa tgatgcaaaa ctgatcctga
      301 tccccatgag taaatgccat gccactatcg tgggagtcga gagcagacgc ctgggagaat
      361 acattgcgga ggctgtggat gagatcgctg agaactgttt ctgagctgac agaggaattg
      421 cgtgtgcttc ccaccggaat gcgcattatt gccattgacg gcagatgcgc cgccgggaag
      481 acaacactgg ctgccagact tgcaaaagaa ctgggcggag atgtgatcca tatggatgat
      541 ttttttctgc cgcctgcact tcggacgcag gagcgcagaa gcgagcccgg cggaaatgtg
      601 cactatgaac gatttttgac ggaagtcata ccgaagcttg catcgggaca agcattttcc
      661 tatcagagat ttgactgttc ccgcatggca ccgggagact ggctcccggt acaaaataac
      721 ggttttgtat tcgtagaagg cgcttacagc tgccatccgg tactgggaga atacatggac
      781 cggaaagtat ttctggacat agaccatgag acgcagacgg agcggatccg cacgaggaac
      841 ggagaggaca tactacagga cttcctgcag ctgtggattc cgttggaaga agcatatttt
      901 caggcatttt ccctagagga gaataccgat tacattataa aacagacgaa aggcgagaca
      961 gatgctgcat cagttttacc cggattatga ggcggattcc gcctatggca tagattacag
     1021 aacattatat gaacaggggt atcgcggaat catttttgat attgacaata cactcgtacc
     1081 gcacggggca ccggcaacac cgcaggcaac agagttcttc ggcagattgc gggaactggg
     1141 atttcagaca ctgctgttat cgaacaacaa ggagccccgc gtaaaaagtt ttgcggatgc
     1201 agtaggtgct tcctatatct ataaggccgg gaaacccgga agagaaggtt atctgcgggc
     1261 tatggaacag atgcataccg gggcggatac cacacttttt gtgggggatc agctgttcac
     1321 cgatgtgtgg ggagctaaaa aatgcgggat catgacttat ctgacgaaac ccattcatcc
     1381 gaaagaagag attcagatcg tactcaagag atatctggaa aagatcgtac tgtttttcta
     1441 ccatagagaa aagcgtcgga aaggaaatca aaatgctgaa tgatatcaat ggatatacca
     1501 gaacctgcgg actgatcgga aacccggtgg agcacaccct gtctccggtg atccacaata
     1561 ccctgtccat ggtgcttggg aaaaatctgg cctatgtccc gttccatgtg gagaccggaa
     1621 gactggagga tgccgtgaag ggtgcttttg cactgaatct gctgggaata aatgtaacgg
     1681 ttccctataa aagcgatgtg atcccgtacc tggatgacat agatccgctg gcatcaaata
     1741 tcggtgctgt aaatacgctg gtccgcacgg aaaagggcta taaaggctac aataccgata
     1801 tgccgggatt gtaccgggcc atgtgcgagg acggcgtcaa agtcaaggga cagaaagtgc
     1861 tgatcctggg agccggagga gtagccagag cggtagccat gatgctgttg gataaggggg
     1921 cgaaagaggc cattatcctg aaccgtacgg tggaaaaggc acagagcata gcggacgagg
     1981 tgaatcacct caccggaaaa aaacggaaat ttgcaaaagc cctgtctata gatgcgtatg
     2041 atacgctgcc ggaaggagaa cgctatctgg caatacaggc caccagtgtg ggtatgtatc
     2101 cggactgtga tgcggcggtg atcgaggatc ccacatttta taagagagta cacaccggat
     2161 atgatctgat cttcaatcca ccgaagaccc ggtttatgga actggtggag gcacagggcg
     2221 gtgaagccta taatggtgcg aagatgcttc tctatcaggg aatcattgct tttgaattgt
     2281 ggacaggctg tcaggtggaa ccctggcttg cagacaaagt ctatgaacgg atgcagaaag
     2341 caaaaaagac agaggacgga aaataaaaaa atgaaacaca aacgaaggaa tatcgtcctg
     2401 atcggcttta tgggatcggg gaagacaaca ctgggactga aactgtccta tctgctacgg
     2461 atgccggtag aagacacgga caagctgatc gagagacagg agggcaggag catcacacag
     2521 atttttgcgg atgacggtga atcctatttc cgggaactgg agacggaact gctgcgaaaa
     2581 tgcggggaac agaaatacga acgtatcctg tctgtgggag gaggcactcc ggtcaatccg
     2641 gtgaaccggc ccctgctgca tcagtgtgga acagtggtat atctgcgagt gtcgccggaa
     2701 gtggtctacg agcggctgaa aaatgacact acaaggcccc tgctacagtg tgaggatcct
     2761 ctgacaagga tccgggaact gctggagatc cgggacaaaa tctatgcgga atgtgcggac
     2821 attatactgg atgtggataa caggcattcc gatgaactgg cggaagagtt acagctgcag
     2881 ttacggaagc agaaagacat acagagaaaa aaggagagaa aaaagatgaa aattctggtg
     2941 atcaacggac cgaatctgaa ttttctggga atccgtgaga aaaagatcta cggaacacag
     3001 gactatcagt atttactgga tctgatcgac aaaaaagcaa aagaaaccgg agaggagatc
     3061 caggtgttcc agagcaacca tgaaggagcc atcatcgaca ggatccagga ggcttattct
     3121 gacggaacgg aggggatcgt gatcaacccc ggtgcctaca cccattacag ctatgccatc
     3181 cgggatgctc tggcaagcgt ggatattccc aaggtggaga ttcatatttc cgatattacg
     3241 agcagagaag agttccgcaa gatttctgtg acagcaccgg tatgtaacag gcagatttac
     3301 ggacagggac tggacggtta tctgcaggct atcgacttcc tgcgggagaa caggcagtag
     3361 tttcccgaga aatttgaaaa aatttttcaa aagtagttga aatgtgcatg tttttatatt
     3421 aaaatgttaa agaattatga cgtgaaaata tttaggagga atacattatg atttctgcag
     3481 gtgatttcag aaatggtatt accattgagt atgataacaa tgtttaccag attatcgaat
     3541 tccagcatgt taagccggga aagggcgcag ctttcgtaag aaccaagctg aagaacatca
     3601 agagtggcgg tgtagtagag aagactttcc gtcctactga gaagtgccct caggcgcgta
     3661 ttgatagaaa ggatatgcag tatctgtatg aagatggcga tctgtactac ttcatggata
     3721 ccgagactta cgatcaggtt gcactgaact ctgagactgt aggcgatgca ctgaagttcg
     3781 taaaagagaa cgagatgtgc aagatctgct cccacaatgg cagcgtattc tccgtggagc
     3841 ctcctctgtt tgtagaactg gagatcactg agaccgagcc cggattcaag ggtgataccg
     3901 ctaccggtgc cagcaagcct gctaccgttg agaccggagc tactgttgca gtacctctgt
     3961 tcgttaacca gggcgacaag atcaagatcg ataccagaac cggtgaatat ctgtctcgtg
     4021 cataattgtt gagactacca ctgttctgag cgatatccga taagagaacc tgtaaggcaa
     4081 taagttatcc tgtttcggga tggcttattg cttttgttat tttctatatt tttacaccgg
     4141 aaataccaca tgggaaaaag caggagggga gcctgcttcg gagaggcatg gaggtattct
     4201 atggagattt ctacatttaa agtaaaggta cagaaggctg tcagcgaagt gttgggacag
     4261 gagtatacag tggaactgcg ggaagtgcag aagaataacg gagtgctgct tcagggactg
     4321 atgatccgta agggacagga caatgtgaca cctacgattt atcttaattc cttctgggaa
     4381 gcttatgagg gtggcgtgac ctttgcagat attattaaga agatcatcag tatctataag
     4441 gaggacggaa tcggccggaa gattgatgtt tccttttttc aggattttga gaaggtaaag
     4501 gagaaactgt gcttccggct ggtgaaccgg gagaagaacc gggagcagct ggaaaaagtg
     4561 ccgtatattc cgatacttga tctggcaatc tgcttctact atgcgtttga cgaagcagga
     4621 gtggacaacg gtatgatccc catctacaga tcccatctgg acaactggaa ggtgacagac
     4681 agggatctgc tggagattgc agtgaaaaat acccccagac tgtttcccgg tgagaccatt
     4741 cccatggagg atgtgctggg agacgcactg caggaacttc cggaggaagt ccggagggaa
     4801 tttttgagga aggtatccat gatgatcctg acgaattccc gcaagactta cggtgcctgc
     4861 agcatcctgt atcccgggat gctggagcag ctggcagagc gtatcggcgg cgattattac
     4921 atgatcccca gttctgttca tgaattcctg ctggttccca gggaacggga acaggacagg
     4981 gaagaactga agaaaatgat cgcagaagtg aaccggacgg agctgccgcc ggaggaagtt
     5041 ctgtcggacc atttatatct gtattgcagc cgggagaggg aagtaaggat agtgtagaat
     5101 accggagtga atattatgtt tgaaaaaaac tgaataattc caacaattta aactggacat
     5161 atgtagtgtt ttatgatatt atagtcgagg tgatgtaaca aatttgtaat atttgcatcc
     5221 gtagtctaat ggatagaacg gaggcctccg acgcctttaa tgcaggttcg attcctgtcg
     5281 gatgcattta catcaccttg attataggga gagagatgag agaagccgct tgcggctttt
     5341 tgcgattaaa gaactttttt tcagaatttg gaaaggacgc aatatgtgga aagaaaaatt
     5401 acattttctt ggaaactata tcataaagca tagtaaagta gttttgccgg ctgtcgttgt
     5461 tgtggcagtg gcagtcaccg tatccgtaag cctgagcatg aacaatcgtc accgtgagga
     5521 gcagcagaac gcagagagcg cagcggcagt gtccagtgag attgctacgg aaccgacaac
     5581 agaggatgta cctttggtag caaatgagga aggtgcggta tatactctgg tagctactta
     5641 ttataatgcc atggcgaccg gtgatgagac aacgttacgt tccgtttgtg atgagatctc
     5701 cgacaaagac atgtatcgtt atctggaact ggcacagtat attgattatt atccgacttt
     5761 ggagatctat accaagaccg gcccggaaga gggttcggtg atcgcttatg tatattacaa
     5821 gattgcattt gtgggtcatg aggaagaggt gcccggttat caggcacttt atatctgcac
     5881 caatgatcag ggagaaatgt atatcaagcg cggcgagaac agtgaggaag tcaacgatta
     5941 tatcaagacg gtaagcacgc aggatgatgt tgtggaattt aacaacaaga tcacagtgga
     6001 atacaatgaa ctgatggtag atcatccgga agtattgcag tatattagtg agctggattc
     6061 tcaggtaagc attgccgtag gagagaagtt ggccaatcag gtagccggag atcagaatac
     6121 agatacctct gcggaaggcg gtgatcaggc ggcagacggt caggatactt ctgccgaagg
     6181 cacagagcag cccgcagaag agcagggatc gcagtatgtg acgacaacta ctaccgtaaa
     6241 tgtcagaagc tccgacagtg agcaggcgga taagctgggt aaagtggcag gaggtacgaa
     6301 gcttcaggta ctggagcagc gcgccaacgg ttggaccaag gtagattatg aaggtaagga
     6361 aggctatatc aagacagagt tcctgcagct ggcagagagc gcatccggtg ccgagaccat
     6421 cggaaccgtg accgctacca ccaatatcaa tgtacgtgca tccgccagtg agactgcaga
     6481 tcgtctggga gttctttccg gtggcgattc cgcggaactg gtcggcacag aaggcgactg
     6541 gagcaagatc aggtacaatg gccagatcgg atatgtcaag agtgaatatg tacaataatg
     6601 agcagtgcca aaaatgaagc ggcaaaaatc tgactgcttg caggcaggat ttttgatgat
     6661 tcttttttga tagggcgaat gcccgacagc gaagaaacgc gaagcgtttt tgagcggtgc
     6721 actgctggct tggaataaaa taagataatt cagggaaagc ttcttaccaa aaacggtaag
     6781 gagctttttc tatgaataaa acaggaaatc acaatctgaa gaaacaggag atcaacatct
     6841 accgggagat ccagcgtaac acggaaatgg ccatgaaggc actggatgcc gttacggaca
     6901 aaatctacga cgacaggctg gcagccgtgg tggcggaaca ggaattacag tactccaggc
     6961 tgcatgacag ggcagtgcag aatctggtgg agggcaaggc ccagacctac cgggagaatg
     7021 ccatggagaa catgatgctg cgctttggaa tcggttataa caccctgctc aacaccagca
     7081 caagtcatat cgcagaaatg atcatcaagg gcagtaataa cggtgtgctg gagatggaga
     7141 aggtgttggc acacaatgaa gagtttgctg gaagcccctg ccgcagtctg gcgaaggaac
     7201 tgatcgagat ggagaatgcc aacatccgga ctctgaaaga atatctatag catacaaaac
     7261 gaccgctttc ggcaatttaa cgtacatcat aacgcacaaa ttgtatactt gcataccttt
     7321 ttgtgcaatt tttatagaaa atggccaaaa acttttacga tttagtgaaa taaagtttat
     7381 tgtacaatta aaaaagtcgt tttataataa agcatatggc aacc
//
LOCUS       DAWDHD010000122         7337 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_594_length_7337_cov_13.473084, whole genome shotgun sequence.
ACCESSION   DAWDHD010000122 DAWDHD010000000
VERSION     DAWDHD010000122.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 7337)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7337)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7337
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_594_length_7337_cov_13.473084"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2067..2166
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1887 a         1897 c         1519 g         1934 t
ORIGIN      
        1 tctaagtcaa gcagggtcat gaaccagtct ttgttcagca gctcgttgta cagacgctcc
       61 aggttctcct ttttgccgac cttgagcact tctttgctga cgatgaagtc tacagcctct
      121 ttgatcacag gactcttctt gtagtagtcc ttagagacat aatctgcctt ctcgtaatgc
      181 ttgatcacag tgtcactgtc cttaccgaag atgtagatat tgtcatcgcc taccagctca
      241 tggatctcta cgtttgcacc gtcactggta cccagtgtta cggcaccgtt taacataaac
      301 ttcatgttac cggttccgga tgcctccttg gatgccaggg atatctgctc ggagatgtcg
      361 caggcaggga tcagcttctc ggcatagctt acgttatagt tctctaccat aacaagcttc
      421 atgtaagggc tgacttcagg atcgttattg ataatgtcgg acattaccag caggagatgg
      481 atgatatcct gagcaatcac atacgcaggc gcagcctttg caccgaagat aaaggtcagt
      541 ggagtagaag gcttcctgcc gcccttaatc tccagatatt tgtgaatgat atacagtgca
      601 ttcatctgct gacgcttata ctcatgcaga cgctttacct gaatatcgaa gatggaatcg
      661 ggattcagct ccacgccctc cttctccttg atataggata ccagttctgc cttcttggtc
      721 ttcttgatgt cgtagatggc atccagtacg gcctgatcgt ccttatgctc taacagcttc
      781 tgtaattcat ccgcatcctt cttgtagccg tcaccaatgg tctgggtcag gaaggcagcc
      841 agctcatggt tgcaggacag caaccatctg cggaaggtaa taccgttggt cttgttgttg
      901 aacttctcgg gatagatctt gtaaaaatga ttgagttcgc tctgctccag gatctcagta
      961 tggatcgctg ccacgccgtt gacactatag ccatagtgga tgtcgatatg tgccatgtgc
     1021 actctcttgt ccttgtcaat gatctgaact ttttcgtcct tatatttctt tgccacacgg
     1081 tcactcagct ccttgatgat gggaaccagc tgaggcacta cttcctcgat ataagccaga
     1141 ggccatttct ccagagcttc tgccagaatg gtgtggttcg tataagcaca ggtgtggctt
     1201 actacgtcga tagcttcatc catagtgaaa ccttcctctg tggtgagaat gcggatcagc
     1261 tcggggataa ccatggtagg atgggtatcg ttgatctgga tcgctacatg ctcgggcaga
     1321 gtatgcagat cataaccctt ttcctttact tccttcagga tcagctgggc accgttgcat
     1381 accatgaagt attcctgata gatacgcagc agattacctg cctcatcgga atcatcggga
     1441 tacaggaaca gggtcaggtt cttctcaatg gcttccttat caaaagtgat gcccttcttt
     1501 accagactct cgtctacggt ctcgacatca aataaatgca gcttgttcag tccattctca
     1561 taacctacga catcaatatc ataaagtacg gaagtcacct tgcggtcacc gaaagatact
     1621 tcgaattttg tgccggtatt gttcagccag gaatccttct ggatccagtc attcttctcg
     1681 gcattctgca ggtgatcctt gaatacctgc ttgaacaatc cgaagtggta attcagaccg
     1741 ataccgtccc cgggaagtcc cagggtagcg atggaatcca ggaagcaggc tgccagtctg
     1801 cctaagcctc cgttacccag agaaggttcg ggttctgcct cttcgatgtc tgccagattc
     1861 ttgccgttct tcttcaggat atcgctcagc tcctcataga tgcctaaatt gatcagattg
     1921 ttggataaca gcttaccgat caggaactcc atggaaatat aatatacctt cttgtctcct
     1981 gtaatggcgg gagtctctgc catcttctct ttggtcagct gcaatactcc ggtgtacagc
     2041 tgtgcatcgg tgcaggcacc gatattnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnntttt tgtgcaaaat agcttaacag ataatgtcct tatagtacat tttcacgata
     2221 ctgtggggca tgattacctg atgcccttcc ccgccgttca tcagctggtg tacctcttcc
     2281 acggctctgc tggagatgct gtagaagtcc tgccttatgg tattcatctg cttgccgaca
     2341 tatcccatca gggtgatacc atcgaagccg cacaccggac ggaaaatatc catatcgatc
     2401 aatgcattca tggcacctat ggccatcaga tcggaagcgc agaccaccac atccttattc
     2461 ttcgcatatt tcaatgccac gtttcttgcc gtcagctcac tgaagtctcc ctgtctgacc
     2521 atcatggtca gatccagatc cttcaccaga cgcttcatcc ccttcaatct ctgatccgtg
     2581 acataaccgt ctcttctgcc cgcaatgtat agcactcgtt tacagttgtc atccagcacg
     2641 gtcttcttcg ccacatcata ctgggcctgt tcctgatcaa tgctgatgga tgtagtcctg
     2701 gaattgacaa tcggcgcatc cactaccaca caggcaaact gtttctcccg gatcagcttc
     2761 tgcagtacct tgtcctcctt ggacatgcca tagatcacca cgccggaaat actctgggac
     2821 agaaaagatc ttgtgatctc ttccgccgtc atccccgcga tcatggacgg aaagatcgtc
     2881 acgacctcca cgttcagttc cttggcctta tccagtgccc ctaccaggta ctgcatgaag
     2941 atctggtcaa tggcctgggt ctgtctattg ggatccagca ccagcgcgat tcttccaaac
     3001 tgcttggagg aaagcgccgc agcaatggaa ttcggaatat aattcaactc cttgatggcg
     3061 tcctgtacct ttttcttcgt ctcttcactg acattaggat agccatttaa gaccttggat
     3121 actgtggaaa tagccactcc ggcatgtttt gcaacttctt tgatcgatac cataaatccc
     3181 ccgttccgtc cgcagctccc tgcggtcgtc attctcggtt accatacaat tacggtttgt
     3241 ttcatttgac gtttacctat atattctcta tactataatt taacgacaaa ttcgtcaatg
     3301 tacagcattt acgaaatata gtgtgatttt ttggaaaagg ttttccaaag cataatacct
     3361 cggacaacgc aagcatcaga aaggctacag ataccatggc agtaatcaaa agtgtggaag
     3421 aaaaatatat gaaggaagct ctgaagcagg cgaaaaaagc ctatgccctg ggggaagtcc
     3481 ccatcggctg tgtcattgta catgaaggta agatcatcgg tcgtggttac aaccggagaa
     3541 atacagacaa gaatacattg gcgcacgcag agatcacagc gatcaacaaa gccagcaaag
     3601 tgatcggcga ctggcgtctg gaagaatgca cgctctatgt gaccctggaa ccctgccaga
     3661 tgtgcgccgg tgccattgtg caggcccgta tccctgaagt agtgatgggc tgcatgaatc
     3721 ccaaggcggg atgtgcaggt tcgatcctga atatactgga aatgccccag tttaaccatc
     3781 aggtgaaggt tacccggggc attctggaaa cggaatgcag ccagatgctg aagacttttt
     3841 ttgaagagct ccgcatccgg aacaaacagg agaaagctgc cagaaaggca tcaggcgatc
     3901 tctcgtaagc tttcttcctg tgtctggttc tgttcggttg tttttcttct atgcttcggt
     3961 aatcgggaaa tcgctcttcc cgtttcataa agtcccatcg gcacaaagag gatcaaaaat
     4021 acatagtacg gaaatacata tctgctctgc gcttcccaga tgatactgaa caggaagcca
     4081 cctactatgc caatcaacgg cagccacaat gctgtcggaa tttctttttt ttgccaccat
     4141 cttccgcaca aagcaattcc aagtacaagt cctgccaagt atacgatact ctggtaataa
     4201 ttggccagct tccatacggt ttcatgtatg ttcccatagt acagactggt aatcccggag
     4261 gacagcggtt ctccatccgt atcaaacgac tctgttgcct tgaggctgga aaacagcggt
     4321 tcgatccact gatcttccag ttttctctta aagaaatctc tggccatagc cggattctcc
     4381 cggaactccc gcaatcgttc tcgaatatat tcctttcctt cctgtgcggc aggctcctgt
     4441 tgaaaatcat gttctgcaaa cgtggtttgc tgatagcgat tgtacacccc ggccatgcca
     4501 tccgtctcct gtaatcccat cgcaatccag agaatgctcg gaatgccaat gtcactggga
     4561 tatccggaac ggtattcata cattacatct accgctttca ccgtcagtgc tgccgtcaga
     4621 agaattgcaa accctgccag cagatattgt cccttctttt tcttcaggca gaccagaact
     4681 gcataaattc ccactccaat gagtatgatc catgtatttt tccgggcaag cacagcaatc
     4741 cctgccacac atgccgccaa cgcaatatac ctcttcttct cataatgttc ataagcagac
     4801 accgcccaga acattaccat tccaaaactg atactgagca cgtccccgta aatatatggt
     4861 aaatacagca aaaaaggaat acagcccaac atcataatac aatatacgat tctaaatatg
     4921 gctctgtctg tattcagttt caggaaaccg tatcctgcca gaatcgccag tacccaccat
     4981 attacatgca ggatttttgc cggagtataa ttgaaatttc caaacagggc aaacaatatc
     5041 tcatacaata ttcccagccc tttttgttgt tggtacatcc ccacataacc tccggagcac
     5101 aacataatga agttgccatc ccggcaatat gctgcaaatg ccgtggcgct gatctgatct
     5161 cctaccggat aatagggatt gttgagaacg tagatacttc ccagcagaaa cgcagtaaca
     5221 ctggttgcca acaatgtata gatgcagatt ttttcctgtt tttcctgatt ttgagcattc
     5281 agccatttat ctccctcata gacaaggaca gatgtcagca ggaaaataac aaaatgtttc
     5341 caggtactgt cttttatcag gagatttctg atgctgtaat ccaccgttcc gtaataagaa
     5401 taccgacagg accagtaact caggaccaaa agaatgggaa tcagacagac aagaaatatt
     5461 cccaacagaa tacggtatat tttttccatt aatgttccct catttttatc tcatagttcc
     5521 acataatttc tgtcaccgac atatactgcc agcttttcat tctcatattc cagtatcggt
     5581 gcttcccctt ccggaaattg ctgttcaaag gcttcacgca tttctgtccg tacgacgtaa
     5641 gcggtccgca tttccttggg cagcgtcggg ggataccatt tcgtactggt gagccaccaa
     5701 tatccggaaa gactggccat ggtataggaa tgtcccatcg tcacccggcc gtccgtgatc
     5761 agacagatcc gttcggcatc ccagaattct gcatatccgt agtcaattcc cgcttctgtc
     5821 agataatccg ccacttcctg atattccttc aggttgtcct gcgtcgtgac cgcatcataa
     5881 taggtgtatt tcagattcaa tactgcgaac agcgctacgg ccgttaagat cagaccagcg
     5941 aacaccggct gtccttccga catatgatcc accatcaccg ctaccagaac agctgctgcg
     6001 aaccatgcca tgaaaaagta ataatgggcc acctccgcgc tggtgatccc gatgataaat
     6061 gctgtgactc ccacagaagc aaggagaatt gtcagtgcat ctttttgtct gtctgtgctc
     6121 tctgtatttt tccggaacac ataccagagt ccatacccca acaggatcag gaacacccag
     6181 acaagaagct gcatcagaga agcaaagctc cctacctttg catttcccac cagtccgaag
     6241 ttctccaaca gggctgccgg aaccccaaga agcagtttct ctactgcctc tcccggctgc
     6301 agcagataga ctgcataatc tcctctgccg ccgtacagac aggataccag aaaagcaagt
     6361 gcaagacttc ccacataggc tgtggcaaga taacgtccac cgggcagctt ctgccggaga
     6421 tgtccggatt ccatgaataa agcgatcact tccactacaa gcagtggcag gatgatgacc
     6481 tgcagacatc tctgtcctcc acagttcagc aaaactgcgg caacaaacaa taccagccac
     6541 tccagaaagg tcttccgggt catctgccgg ttttctttta atttcaggta aaacaggata
     6601 ttgaaaaaca gaaaaatgcc atgaaacaca taatactctg caaataggaa taaaagcagc
     6661 gctacaaaag gaaccatctg cccctcattg cgaaaaccgt tgattggcag cgcacacaat
     6721 aacagcaatg ccgtaattga tgccggtctg ctcaaagata attttctcag aaaaaaataa
     6781 aatgtcccca gcaaaatcgc acccaataga acacaggtaa tccccgccgc cgtctgcatg
     6841 ctgccggtga taccgtaaaa tacggctgca accgtaggca ttccaatgat ccgtctctcc
     6901 gtgctggaga tccagtcgtt gggtgtaata tccttctcca cccagatctc ccgggccagc
     6961 aaagcctctg cggcaatatc cgaatccaca tgttgtgcat atttacacag attcaggtag
     7021 caaaatactc ccagatatat cagtaaaagt gcacctatta tccatcccag cagggatata
     7081 ctttttcgct tgtttctcat ggatccttct ccgtaggcaa ctatctttcg acaaaattac
     7141 atattatttt aattataaca ctcttccctg tttccttcaa ggcaaagagg cttgacaaaa
     7201 accggggaac aggatacact aaaaaggccg tacatgtccg gtatcatggg aatagtgatg
     7261 aaactcggtc ttacgcaatg ggaatctacg aaccgtgtca ggttgggaac aaagcagcac
     7321 taagtagaac cttccat
//
LOCUS       DAWDHD010000123         7263 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_604_length_7263_cov_7.598224, whole genome shotgun sequence.
ACCESSION   DAWDHD010000123 DAWDHD010000000
VERSION     DAWDHD010000123.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 7263)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7263)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7263
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_604_length_7263_cov_7.598224"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2049 a         1519 c         2136 g         1559 t
ORIGIN      
        1 gctgactccc tgggagaaga tcgaaaagag actgcgggcg gcagcaatgg gagattttgc
       61 catggcaatt tacaaccctt ccagccacag gcggaaggat tatctgcaaa gagcctgtga
      121 tattttactg gaaaccatag aaggggagcg cccctgcggc tatgtggaaa acatcggcag
      181 agaaggcacc cgggcggtga cctgtacgct gcgggaatta agggaccggg aagtcaatat
      241 gttcaccacg gtatttatcg gcaattccga gacggagatc ctgggggaca agctgagctc
      301 cagaagaggc taccaggcag aaaagggaga aaaggcacag tgaaaaagct tgtaatattt
      361 gcgggaacca cagaaggacg gagattgtcg gagattctgg cggatgccgg aatcgcacat
      421 acggtatgcg tggcaacgga atacggtgaa atcgtaatgc gggaacagac ggaaagcaca
      481 aaagcgatgc agacagccgg gcagcccatt gtaaaccttc actgcggcag aatggatcgg
      541 gagcagatgc agaagtttct gcatgacgaa ggctatgaga ttgtagtgga tgccacccac
      601 ccttacgcca gggtcgttac ggagaatctg cggggagctg tggatacact gaaatctttg
      661 gaaaaagatg cacattttcc catttacctg cgtctggaac ggaaaatcga cgggattaca
      721 gaagccgctg gcattgttac aaatgtccaa tattttgaaa acaatgcaga ttgtgcgaaa
      781 gctttggaga atacagaggg caatattctg ctgaccacgg gaagcaagga actggcggtg
      841 tattgcgctt ccggcagatt gaaagaccgc ctgcatgtgc ggatccttcc gggaagagag
      901 agtctggaac agtgcatgga gcagggaatc aaaggccggc agatcctggc attgcagggg
      961 cctttttcca cagagatgaa cgctgccatc ctaaggcagt acgacatccg ccatatggtc
     1021 acgaaaaaca gcggccgggc cggaggctgc caggagaagc tggaagcggc gaaaatgctg
     1081 ggaatcccgg tgtatgtgat tgagcctgcg cagaagacat cggatgctgc cgggaatatt
     1141 ccggggacag actcgtattc gtttgcagga atctgtggaa aactgggaca actctgtgat
     1201 tgtaagattt cgcagcaggg aagtctggaa atctgccttg ccgggatcgg tatgggcagc
     1261 agagactgca tgacaaaaga agtgcagcag acaatcgaaa ctgcggatat cctgctgggg
     1321 gcggagcgca tgattgcaga ctattctgcc agaatagaaa aaaagcccta ctatatggct
     1381 gcccagatcc tgccctatct ggaaaaatta caggaaagcg aggaactggc acagcaggag
     1441 acacttcggg tagcggttct gttttccgga gataccggat tctacagcgg atgcagaaaa
     1501 ttgtatgtgg aattacagga agcgattgca gcaggacaac tgagaggcag tgtgaggatc
     1561 ctgccgggaa tttcatcggt ggtttctctg gcggcccggg tgggagaaag ctatgaggat
     1621 gccgccattt taagcatgca cggcaagaaa ctgaaccgac tggcggcaac ggtggagagc
     1681 catgaaaagg tatttttact gacttcgggt tgtgaagata ttcgtaagat cggacagacg
     1741 ttgacagagg caggactggg ggactgcgaa gtgatcgcag gatatcagct gtcctatccg
     1801 gaggaaaaca tacgtatcct gacaccggag cagtgtgaag aggtcacgga gaaaggcctg
     1861 tatacctgcc tgatccgcaa tcctcatcca cagccggaac ccctgaccca cggcagagcg
     1921 gacacctgtt tcctacggga tgcttccggg acggggggaa aacttcgcag aactcctatg
     1981 accaaggaag aagtccggga agtcagtatt tgcaagctgc acctgacaga tggagccgta
     2041 gtctatgata tcggcagcgg caccggatcc gtggcggtgg aaatcgccgg attgtcggaa
     2101 acggtgcatg tctatgccat tgagagaaaa ccggaggcgg tggaactgct tcggaagaac
     2161 cgagagcatt tccatatgga caatatggag atcatcgaag cactggcacc ggagggactg
     2221 gaagaactgc ctgtgccgac ccatgctttt atcggcggca gtggaggcag attaatggat
     2281 attttacaga ccttgtatcg gaaaaatcct catatgcgca ttgtgatcaa tgccatcagc
     2341 atggagacga ttgcggagct tcaggaagta ttggaagcgt ttcctgtgga ggacgaggaa
     2401 atcctgcaga tgcaggtcaa ccgggtgaag aagctgggaa actatcatct gccgcaggcg
     2461 gagaatccgg tgtggatctg ctcatttacg ttcagggagg gagaaacagg ctctatggac
     2521 aaagtgaaga aagcgttgct gaccgaagaa gaaaacggaa acagggaaaa tggagaagtg
     2581 cagagatgat cggagcacag gaaaaaaaga tacgacgcat catgatcgcc gccccgaaaa
     2641 gcggcagcgg caagactatg atcacgtgtg gattattgca gatttttaag gaaaacggag
     2701 aggatatctc ttcctgtaaa tgcggcccag attatatcga tcccatgttc cacagacagg
     2761 tgctggggat tcctgccaga aatctggaca cttttttcac cggagaggac ggcacgagaa
     2821 gcctgtttct gagagatcgc agggaagacg agcttgtggt catggaaggt gttatgggac
     2881 tctatgacgg gctgggcgga ataagagagg aaggctcctc ctatcatctg gcaaaggtga
     2941 cgcagacccc catcatactg gtggtggatg caaaaggcat gggaaaatcg gtgattcctc
     3001 tgatcaccgg atttctggct tatgataaag aacatctgat ccggggagtg atcttcaacc
     3061 gtatgtcggc agtttactat gagattctga agcctctggc ggaagaggaa cttggtattg
     3121 cagtgctggg atatttcccg gaaaataagg acttacagat cgccagcaga catcttggcc
     3181 tgtgcctgcc gggagaactg gaagatctgc agggaaagat ccgggtcacg gcggcaaaac
     3241 taagggagag cgtagacatt tcgaagctgt tgcagattgc agaagaggcg gaaccgctgg
     3301 gagctgagag aatagagaga acctcccaaa ccaaaaagaa ggaacgcagt gaagaaacgg
     3361 cagaagagca agatccggat tctgtggaaa acgatagggt aaagtgcccg aggatcgctg
     3421 tagcccggga tgaagccttc tgcttttatt atgaagagaa tctgcgcctg ctggaacaga
     3481 ccggagcaga actggtgtat ttttctccat tacatgacaa ggcgttgccg gaaaatatcc
     3541 atgggatgct gttgggcggc ggttatccgg aattatatgc cggacagctg agtgagaaca
     3601 atgccatgcg caaggccatc cgggcagcag tcataggtgg cctccccacc gtagcggagt
     3661 gcggaggctt tttatacctg catacgtccc tgacagacag agaaggacat tcctgcctta
     3721 tggcaggagt gctgcctgaa aaatgcttcg atactggaag cctggtgcgc ttcggatata
     3781 tcgaactgga agagaacagt gaatattttc tgccccgggg cagccgcatc cgggcacatg
     3841 aatttcatta ctatgacagt gaagataacg gcgtagactg taccgcaact aaaccaacca
     3901 tcggaagaaa atatccctgc atccatgtcg gtgagaacca ctggtatggt ttcccccacc
     3961 tctattatcc ttcctgcccc gagttcgcag agcaatttgt ggaaaaggct gcgcagtaca
     4021 aaaatcgttt taaatagcag gatatttggc ggtatctgta aaaattgtgc aagaaaaata
     4081 aaaaaacact tgcatcgtac attgatgtac ggtgtacaaa gaaataggga agtatttcgg
     4141 atgaaatacg ttaaaaggac gaaaccggct ggcgagagtt gggttcgtcc ttttttgcag
     4201 aaaaattctc aaaaacaaaa aagatgtgtt ataatcgtaa tatcttttta ggtacggagg
     4261 ataaagttat ggaaaaatca aaggtatatt ttacagattt cagaacccgt ctgggagagg
     4321 gacttcccag taagttaaag cgcctgatga aggcggcggg gattcaggat atggatatgg
     4381 atcacaagtt tgttgccata aagatgcact tcggtgagct gggtaatctg ggattcttac
     4441 gtcccaatta tgcgaaagcg gtagcggatg tggtaaagga gctgggcgga attcctttcc
     4501 tgacggactg taataccctg tatcccggca gccgtaagaa tgccatcgag catatgtact
     4561 gtgcctggga gaatggtttc acaccgttga cggttgggtg tcctgtgatc attggtgatg
     4621 gcctgaaggg aacggatgac atcgaggtgc cggtagaggg cggcgaatat gtgaaaaatg
     4681 ctaagatcgg ccgggccatt atggatgcgg atgtgtttat ttccctgaat catttcaagg
     4741 ggcatgaaac ggcgggcttc ggcggtgcta tcaaaaatat cggtatgggc tgcggatcca
     4801 gagccggtaa aatggaacag cacgctcagg gcaaaccgga gatcaatgag aatttgtgcc
     4861 gtggctgcaa acgatgcatg agagaatgtg ccaatgaggg actggtatac gacgagacta
     4921 cccataagat gcatattgat cataacaaat gcctgggctg tggacgctgt atcggtgcct
     4981 gtaacttcga cgcaatccat agcggttgtg atgcggcaac aaaggatttc aactgccgta
     5041 tggcagagta tgcgaaggcg gtcgtggacg gcagacccaa tttccatatt tctctggtgg
     5101 tggatgtgtc tccgaactgt gactgccacc gggagaatga tgtacccatc ctgcccaata
     5161 tcggtatgtt cgcctccttc gatcctctgg cgctggatca ggcctgtgtg gatgcctgcc
     5221 tgaagcagac accgctgccg aacagccagc tgacggatgc catggcaaaa gagggctttt
     5281 gtgatcacca tgatcatttt gaaaatacca ctccaaatgc ggagtacaag acctgtctgg
     5341 agcatgcgga gaagatcggt atcggcagca gggcgtacga acttattgtt atgaagtaat
     5401 ttcagaaagg atgagcttat gcgtcccatg gtaagcatca ttgttccggt atacaatgcg
     5461 gagcaatatt tacgacgctg tgtcgacagc attttgaatc aggaatacac ggactttgaa
     5521 gtgtttctgg taaacgacgg cagtacggat tcctccggtg acatctgcga ggaatacggc
     5581 aataaggaca caagagtcat tgtgatccag aaggaaaata ccggagtctc cgacagtaga
     5641 aatctggcat tggatcgtgc acggggaaaa tatctgcaat tcttagacag cgacgactgg
     5701 atcacgccgg atgccacaag actgttcgtg cgggcggcgg aagagtatgg ctgcgatatg
     5761 gtcatttcag acttttaccg cgtggtggga gagcgtctgt cccccaaggg agacattgaa
     5821 gaggagggtg tgctcaccca ggaggagttc gcggcacata tgatggagaa tccggcagac
     5881 ttttactatg gtgtgttgtg gaacaaactg taccgcaggg atctggtgga ggagcacagt
     5941 ctgcgaatgg atacgaatat caactggtgt gaggattttc tgttcaatct ggaatatatc
     6001 cgttacgcga aagtattcta tgccctgcat gccccaatct attattatgt gaagcgcaaa
     6061 ggctcccttg ccagtcaggg aatcagcatc tccaagacgg taaagatgaa gttgaatgtc
     6121 tttgagtatt acaataactt ctacaagcat gtgctggaag aagaggacta cgagaagaac
     6181 cgtttgcagg tatatcgttt cttcattgat gcggcaggag acggaaacgt gccgccttcc
     6241 atcctgcccg gatccaagaa gctgggagac gagagagtct ttgtcaacac cgagatcctg
     6301 caggccgagg gaccggcggg ggatgcttac cgtaagcgga agcttctgga acattatctg
     6361 gagccggtgg cattaaaagg agatctgaag gtagcggatg tgcggctgct tctgtgcctg
     6421 tgtgaaaagc acgagtggga cagcagacgg gaactggcgg atttcgccgg aatctcccgc
     6481 acgaacctga ccagcggctt gcaacgcctg accatgaagg gattcttgaa aatagaggaa
     6541 gtgaaggaac ccaagccttc cagaaaaagc aaggcagccg gagaagtaca gggaaaagcg
     6601 gaaaagccgg agacaaggcg caaagaccgt aaaaccaagg tcacagtgac aatccttccc
     6661 actgcggaag cggtcatgaa ggaactggca ctggcacagc gggattatga tgaggcgaga
     6721 tttgtgggat ttaccgagga agaactgata aaatacgcag atctgtcgga gaaaataaaa
     6781 gaaaatacaa agaggattct gggataattt tatactttgt taattgacat cgccccttcc
     6841 aaaaagttat gataaataag tatataaaaa tgtgagggaa aggaaggtat ttggatgaag
     6901 aaaaacgaag agtatatgtt ggaacatgcc ggattgtgca gagcggcaga tagattggaa
     6961 ccccagatgt tcgacgaata cggcgtacag aggggactgc gtgataagaa cggtaacggc
     7021 gtagtcgcag gtcttaccaa tatttcacgg atcgaatcct tcaaaatgga agacggcaaa
     7081 aaagttccct gtgaaggtaa gctgtggtat cgcggctatg actgcatcga gctggtcaac
     7141 ggcttccggg gtgatcattt cggattcgag gaagtggctt atttacttct gatcggatag
     7201 ctgccgaacc gtgaggaact gaaacaattt accgacattc ttgcgtccag gagatctctg
     7261 cgg
//
LOCUS       DAWDHD010000124         7218 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_608_length_7218_cov_9.437247, whole genome shotgun sequence.
ACCESSION   DAWDHD010000124 DAWDHD010000000
VERSION     DAWDHD010000124.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 7218)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7218)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7218
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_608_length_7218_cov_9.437247"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1766 a         2047 c         1572 g         1833 t
ORIGIN      
        1 tacattgaaa tcaacactac ggaagatacc gcagtagcgg gctaaccccc gctacgaacc
       61 cctttgacag tgtgttttcc taataggcgc taatcatata aggagtccct atgatcgcat
      121 tacagattac atccatgaaa aattttatga accacctgct ggtagcggat actttcgatc
      181 ctttcctgtt ggaagaagcc accgtcagca cggctctcac cgttaccatt gacgggcata
      241 tcaacaagga cttctatccc gcagaggaac ggagtgcaga ctgtatcccc tgggagctgc
      301 agtcctggag caaaataaaa ggactgtgct ttgacctcat caagggaaaa cacactcctt
      361 tgtattttaa attcgtactt catatgcggc ccgataaggc agcctccatg ctggaaaaag
      421 cgcaggtcga tccgggcgtc atccgggcgc tggtattaaa tatccgctac gacggggaaa
      481 agaccgttct caccaccggg tgcgcctacc agaccttcac catggataaa agtgccgaca
      541 ccgcatggga cagggcatta aaaaaatatc tggatctgaa gggtatctcc tacgaagaac
      601 tctgatcccg ctctccgggg tatccccagt tcttcgaatc attcgtagga gcggttcctg
      661 acggtcactc cttcatcttc ggtttgctga gcaggctcgc cagataaccg aggatcagcg
      721 ctgcggaggt tcccacggct ccggccttga atcctccggc aaaaagtcct aaaaatccct
      781 gttcctgcac cgcttctttc acgcccttca ttaatacatt tccaaagccc aataacggta
      841 cactggctcc tgctcccgca taatcctgaa acggttcata ccagccaagt gcactgatca
      901 ccgcaccggt gaccaccaga agcaccatga tgcgtcccgg catcatcttc gttttttcca
      961 ttaatatctg caccaaagca cagatcaggc cgcccaccca gaaagcattg atataatcca
     1021 ttctctgcat ttccccctta ctatctgttt aaggacagtc tctgcacttt ttcttaaaat
     1081 tatgcattct ttatttatgc attctttttg ccaaaacgaa accattccgg cggaagctgt
     1141 accaggatca ccgccgcaaa tacgataacg cagcctgcga tctgcctggc tgtcagtgtc
     1201 tgatcagtag tcagaaatcc gatcttatag gcgatccatc cggagatcgt ggcaatgacg
     1261 gattccaggc tcatgatcag tgccgccacc gtaggattca gtcctttctg gcccacgatc
     1321 tgtaaggtat aacccacacc acagcttaaa acaccggcat ataacagggc tcccaggcct
     1381 tcctgcagct gaccgaaggt gggctgtccc cagatgagtg ctccgatgca gcagaccact
     1441 ccacagaccg tgaactggat acaggatacc acagctccgt caattcttgc tgcataatgg
     1501 tcaacggtca tgatctgtat ggcaaatgca atggcacata aaaataccat gatatctccg
     1561 gctccgagac gcagggactc ggtcatgcag agcagataca tgcccacggc tgccatgact
     1621 gctgccgccc agaccaccgg cgccactttt ttcttcagaa agataccgaa catggggaca
     1681 aaaatgatat atagcgttgt gataaatccc gccttaccga cactggtgta tttaataccg
     1741 aactgctgca aggtactggc gatcgttagg aaaatgccac acaaaagtcc tgctttcaca
     1801 ttcagtttcc agtcaagggg cgcagctttg tcccctgcaa tctttctgct gatcagaata
     1861 tacaccagaa tgctggcggc accgatcagg gaacggacac cgttaaaggt gaacggatgc
     1921 atatagtcca tgcccttgct ctgggagacg aacgccatgc cccagatggc agccgttaac
     1981 agcaggatca ggctgtttct cgtagttttt gttctcgtat tttccatagt tgtattttcc
     2041 attccttata tttttcgttc cttgctgctt tacggttatg tgatcttgtc agggctgttt
     2101 cttatatctc tcagcaatct ccgccactac atcccgcagc atttttctgc tacagtctat
     2161 gatcagatcc gcatattttt catacaaagg ggttctctcc tcgtacaggt ccctcaatgt
     2221 ctgaccgggc tgcagggtca cacctctggc attcagatct cccagccgct gttccacttc
     2281 ctcataagga agcttcaaat ataccacggt gctgatatcc cgcagatgct gcatgccctc
     2341 cggctcgtag caggcacttc cgccggtggc aatgatgctg ttttccagat tcagagaggc
     2401 atttacgttg ctctcgatct gccagaagcc ctccactccg tgttccgtga tgagttcatg
     2461 caataattta ccgtgtcttt cctggatcac cagatccgaa tccacaaaac gtctgcccag
     2521 gcgctttgcc agaaccacgc ctaccgtact ttttcccgaa cccggcatgc cgatcagagt
     2581 aatattgttt ttcataaatt tctgtccacc ttacctatac acaagaaaat cgccgcctgt
     2641 aagcgacgat cttccgtcct gttcttcttt ataaccttcg tagcgcccca tttgcagaac
     2701 tgcctctgtg gggattttga ccccgacatc atgctatttg ctcatggtgg ttacttacac
     2761 tttatacttc cgcaatgact tccacttcac ataatacgtt cttgggaagg gtcttgactg
     2821 caacgcagct tctggcagga ttctcagtaa agtactttgc atagacctga ttgaaagctg
     2881 cgaaatcgcc catatctgcc aggaaacagg aagtcttggc cactctggtg taatcggtgc
     2941 ctgctgcctt taacagctcg ccgatattct tcattacctg ctctgcctgt tcctcgatgg
     3001 tagttccgtg aatctctccg gtagcgggat caatagggat ctgtccggat gcgaaaaaga
     3061 tattgcccag tacaatgccc tgagagtaag gtccgattgc tgcgggtgct ttgtctgttg
     3121 aaatcttctg taacataata ttctcctctt cttcctataa ttacgccctg aggatgcctc
     3181 cggatcccgg cacatccaaa atccgatgca gcatgcacat agcgtttact tcgtcatatc
     3241 tacctccggc tcatcaaatt ccgccgtcac gtagaatcct ctggaattct ccacgatatc
     3301 cgccacaact ccttcgcgat tcttcaaccg ataacgaaaa ggaatattgg gggacatggc
     3361 cagagacggg tctatcgtat aataatagtt actgggcaga agtccctcgg tcactgtgat
     3421 cttctgtccg acctccaaca gtcccggccg ttccatctta aattctatcc tgcgcatttt
     3481 gactcaccag ccattcccgg cataatgtgt tttcgccgtt tgaggtttat tttaccagta
     3541 acttacagcg cttgcaagag tgtgtttcat ttctgacctg ctgcttttcc ctatttcacc
     3601 gccggactct ccaatactac cgcatgtgcg atccccggca cgcttttacc ttcgttgaaa
     3661 ctggtcttac tgagcagggc gccggtgggt agaaagagga cttttttcca ctcgccggat
     3721 tccagcttcg gcaggatata tgccgccagg gtcacagcgc tgcatccaca gccgctgcct
     3781 ccggcgtgtg tgtcctgggc ttctgcgtca aaaatctcga taccgcagtc catgtgccgg
     3841 tcggcgatgt caattccctt ttcccgcaaa agatcgatca ataccgtctg tcccacgctt
     3901 cccagatccc cggtgatgat ccggtcatat tcttccgggg tcacatgaaa atcttccatg
     3961 tgacgtgcaa cggtgtctgc agcggccggg gccatgcatg ctcccatgtt catggagtct
     4021 ttcaggccgt aatccacgat ccttccggga gtcatgccgg tgatgtgggc tctgcacttc
     4081 tgatttagtc ccaatgcaaa ggctccgctt cccgtcaccg tccagctggc agacagaggt
     4141 ctctggctcc cataatctaa cggaaaacgg aactccttct ccgcactggc aaaatgactg
     4201 gaggtcacac agaccacatg ctccgcatag cctcccgctg ccatcacagc tcccagtgtc
     4261 agtgcttccc cgcaggtaga gcaggcgccg tagactccaa acagaggaat attgtaagcg
     4321 gagataccaa aggaagtggc aatgcactgc cccaagagat caccggcaaa gagatacgac
     4381 acatcctccg ctttccatcc ggccttttcc atgaccatgt agactgcgtc cttctggaga
     4441 ttactttccg cctcttccca ggtcttacag ccgaagagat catcctctcc caccatgtcg
     4501 aacagggtgc ctaacggtcc ttcgccttct ttttttccca cgatactggc accggcgatc
     4561 acacacaccg gctgtgccaa acgaatactg cttcttccca ctctctgatt cataatttct
     4621 ccttccatgc gataacactg cattcttttt tgtcagtgtt cccgcattag ggaagaatta
     4681 tgaattacaa aaaattgtaa ctattcagaa ccccattcgc aaaaccgcta cttcgcagct
     4741 ttccttttgc tcatgtggtt acttcgccat ataaaactgc ttatctgcct gaatgcaagc
     4801 attcgcagaa cgcagttttt catggctctg aatagttaca aaatatctcc tccagcgccc
     4861 cgatcacctt ctgtaatatc tcatccgcca tatccgaata gtgaatgacc agaatccccc
     4921 gatatttctg cgcatccgca aagcaatatt ccgacagaca gttcacgaga attccccgct
     4981 gtctggccgc ccacttgatc tccacgtcac taagttccgt ccgcacatgc agcagaaaat
     5041 gagtcccggc atcatcctga tagatctggc tgatctgact taaaggtgac tcccgaaaca
     5101 tccggcagat ccgctcccgc tgccctctgt aatcgttgag cagtctccgg atatggcgtt
     5161 caaaataccc tttttccaga aacgctgcca tggcatactg ttcaaaaccg gataccgtgc
     5221 aggaataaaa attcatagtg gaaatatagc gttccatcaa tttctccggc agcaccatat
     5281 agccgatacg cagggatggc accatggtct tggaaaaagt gttgatgtaa ataactctgt
     5341 ggttgcgatc catggactgc atggagggaa ccggctttcc caccatgcgg aattcacaat
     5401 cgaaatcgtc ctccacgata tagcgctccg gttcctccgc cgcccagcga agcagttcct
     5461 gccgtcttcc cacaggcatg accagtccga tgggaaaatg atgttccggc gatacatgcg
     5521 ccacggtaat acccttttga tccagctgtt ccactttcat tccctgactg tcaatgtcca
     5581 cataatccca ggtcacaccg cccacatcat acagtttcgt aatcttgcga tacccaggat
     5641 tttccacacc gaattttgca tttttgccca ataactgtag cagacgactg tagagatatt
     5701 ctgttcccgc tcccacaata atatgatccg gtgacacatg catgccacgg taccgataca
     5761 aatgctctgc aatggccacc cttagttttt ccactccgtt aaaaggaacc gtcttaagca
     5821 gagacgtatc ataatccgtc agggtctgcc gcatgatctt cgcccaggtg gcaaagggga
     5881 acttatcata ttgaatattg ttggccgtca gatccgccag atattcttcc tcccggaaac
     5941 gggtcacaaa ggatgcatag gctggggcgg agacgttctt cacttccaga cggttgacaa
     6001 aataccctct cttttcctcc gaccggatat agccctccag cagcagctgt tcataggtat
     6061 tctccacggt cttgacactg acctgtaagt gctcggcaaa cgcccgcttc gaaggaagct
     6121 tctcaccggc agtcagccgg ccgctcaaga tgtcagcttt catcgcttca tataagtatt
     6181 catacatggt ttttctgcca cgggcagtca gatcataggt cagcatctgg tatcctcctt
     6241 ttttcttatt ttggttattt aaaagatatc agattttatt ataatgaact cataaagtaa
     6301 aagcaagcag gaaggacaga ttctcccccc gccaaggggg tgactcctca agagggcagc
     6361 tcctcaagaa gagcaggatt ttccttcgga aaacaaggga ggatttaaca tgggcgtata
     6421 tgcaatcacc ggcgcatcca gcggaatcgg cgccaagaca aaagaactgt tacttcaaaa
     6481 aggacacgaa gtcatcaata tcgatctgaa ggacggcgat atctgcgtga acctggcaac
     6541 cccggaagga agacaggccg ctgtagacaa gctgcatgag ctgcatcccg aaggtctgga
     6601 cggcatgatc tgcaatgccg gtgtctccgg tgcctgcggc aatctggaac tgatcatttc
     6661 cctgaactat ttcggaaccg tagccattgc aaagggcgtc tatgacctcc ttaagaaaaa
     6721 acacggcagc tgcgtagtca ccgtatccaa caccatttcc cagggtgcgg gacgtaaaga
     6781 tatcgtagat ctgctgaaca atatcggtga tgaaaagaga gtattaagcc tggtatcctc
     6841 catggattcc accaacctct ccgtgggcaa cagtctctat gtatccacca aatatgctct
     6901 ggccagatgg gtccgcagag tctccgctac ctgggctgcc aacgatgtcc gcatcaatgc
     6961 ggtagctccc ggcaacgtac atactgccat gaccgctacc atgagcacca ccgcgaaaat
     7021 ggcactgaat gccctaccca ttcccaccaa gtacggtcag gaatgtctga tggctcccga
     7081 agagatcgct gaggtgatgg tattcctggc atccgattcc gccagaggtg tcaacggcaa
     7141 catcatgttc gtagacggcg gtactgacgc actgttaaat tctgaaaagg tatattaagg
     7201 aggaacacac tatgagac
//
LOCUS       DAWDHD010000125         7166 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_613_length_7166_cov_10.541415, whole genome shotgun sequence.
ACCESSION   DAWDHD010000125 DAWDHD010000000
VERSION     DAWDHD010000125.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 7166)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7166)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7166
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_613_length_7166_cov_10.541415"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5205..5304
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1601 a         1905 c         1472 g         2088 t
ORIGIN      
        1 gcattcaggg acaacagatt cgtctgggat gcgatatcct caatctcacc gatactatcg
       61 ctgatctgcc tggtagtatc gttgatccgc tgcatggcat ctgtcacacc cttcatttcc
      121 tctccgctgt ccttcgcctg ctgctggtaa tccatagcct tctggtaaga acctcccagt
      181 gccttggcat tcttctccac gatctcggtt acattcgtga tggtagcctg cagttcctct
      241 acggaaccgg cctggtcggt agcaccttcc gcaagtccct gggcagcatc tgccatctgg
      301 tttgccccca gagatacctg tcccactgcc gtcttgatct cattaagtgt ctcactgagc
      361 ttatggttca gcttacggat agattccaac agtccctgaa agtctcccac ataggcttct
      421 gcgcaacctg tagtcagtgc aaaattgccc tgtgacatct ctcccaacag ataatccgca
      481 tcgccaatca ctttctgcag gttaccggat aatgcggcgg tagcctctgc caggatctgt
      541 gtctcatcct gtgtccggat cactggtgcc ggagtatgca gatcgccctc agccaacaga
      601 cgtaatctct cggtacactt tgccaccggt tcaccgatcg cctgaccgat cttttttgcc
      661 ctgcgcacac tgaaacataa agctgcagca cctattgcta caccaatgac aatacataca
      721 acggtagaac caaggaaatc tgtcagtggt gcatttactg ccacgctcca tccattggtt
      781 ccttctattg gtgcataagc ctgtattgtg agggttccgc ctttcatata agtgccatat
      841 ccggttgctc cggtcatcat cttctgctct acctttgcaa ttccccgcaa agatctgtcc
      901 gtctgagaca gttcaatgtt gctcttctgc tcctgggcaa tcgcagaatc acagtgagca
      961 atggtgatac ctgtttctcc caacagatag cactcggaat tcttactgac agagactgcc
     1021 gcagcaatat catttaagaa ctccggatcc ggcacaacga ataccacgcc gacaatatca
     1081 ccattcttat ctccgtctgc atatacgggt gcggaaatga taacgctgag cttaccgtct
     1141 gtctttgcaa tcacgggatc cgataccacg acctctccct gcatggatct cttaaaataa
     1201 tcacggtcac tataatcggt tccgtcataa gcacagatac cgttggagcc gatcagcttg
     1261 cctctgacca ttccataagc ttccacacgc tggttgatat actgttgctt ctgctcctgg
     1321 gtaaataccg gagaagacag ctgatagctg catccgagtt cggatacgat cgttgttgtc
     1381 acccgtagtt cctctttgat acggtctgca gagatctctg ccaccaccag catatcgcct
     1441 tccagtgttc tcatggtact ggacagattc aggatcacgg taaggatgac caacggaata
     1501 atcgagaaca ctaccaacct gctgattgcc ttctgcagac gttcccggat cgtcgtcatg
     1561 ccgggtgtct gcccctttgt gcccttctgt gatgcttttg tactcatatg tcctcatttc
     1621 tgcgctgcat ttttattata tgctttattt tcctgtactc tgtgaagtga ccacttgtcc
     1681 ggcagcgccc ggatattcca ttcatctccc tcatttggaa cagtgatctt ctgcagagat
     1741 actcaatttt atatgtcgat tttattttac catgattcag gaaaattttc tatcttttga
     1801 agcccaaaaa gagagttaat attccgttaa gaatatcaac tctctcctgt catttatctc
     1861 tctatcccct catgataaga aataccctgt taatagccga actcctgggc aaaatacaac
     1921 ttgccattgg tctcatagac cgcaatgctg caggtggtaa aatcgcctgc caggatattc
     1981 gctgcgtgtg taggagaagc catccaggca tctactacac tctgtgcgga atcatagccc
     2041 ttcgccagat tctcgcccca catcagttca ctgttcactg tgtaccagtc cttaccgttg
     2101 ggtctggtat gggaaaactt ggaagctgct tcctgtgctc ttaccatagc gcaggactcc
     2161 agaccgttgc tccagttcag tgctgccagg ccgttctctg ctctcttcgc gtttacaata
     2221 tccaacgcat cgatagccat ggcacgaagt tcatctgcac gggccagctc agcttcactc
     2281 ataccggctt ttctcacaga acccgccagt gccactgcct cttcatcgaa ccagacagtt
     2341 ccgtcatccg tggcaggatc acggcttaca gaggataatt ctgtactctt gccgcagcct
     2401 gcaaggctca gcaccatgat gactgtcaga acaagactca tccatctttt tttcatttgt
     2461 tctacctctt ctttccttga ttgtttatag taaatatatg tttattcgtt atgattgctt
     2521 cttcttaata ataagccatg ctcccagccc tgtcaaggaa agggcaaagc cccgattcca
     2581 tgtattggaa tcatcgccgg tcctgggtct tctggaagct cccaggactg ctctgtccgc
     2641 caatggtgta ggatcatcgg taatgcttac aacccgctga cgtcttacac caagcacctg
     2701 aggtgttacc ggggttgccg gctcagacgg tgtaccggga gtatccgggg tatcggaatc
     2761 atccgaatcg tccggagtat ccgggggtgc ggtcggagta gtggtcggcg tcgtgggtgt
     2821 ggtatccggt gtgcctgtcg gcgtaggctc cggagcagca ctgggagtcg gttccgggct
     2881 ggtagtcacg ggcggagtaa cctccggctc tctggacaat tcaaactgca ttccgaagcc
     2941 gtatctggtc atctgatagc cgttgttggc caacacaccg ttaaggttag tggtgatctt
     3001 cacttctccg tcctttaaca ggtcggatac cagagtatct gcataggaac cttcctgtcc
     3061 gtttttcagc atccggtcat acagaccttc accgtcaaaa gtatatacat tatgatatgt
     3121 cgtgtaatat aaagcatctg ctactgcaga acagctctcc gctactgtgg tatcggcggt
     3181 tccgttggtc aggattgcca gttcctctgc cgtaagttct gaaaagccgt agaccttatt
     3241 tccttcttct ctggaagtat tgttgaacca gtctaagtaa tactgtgcca ggcagctctg
     3301 cgtaacctct tccggcttca tttcttcgct gccatatcct tttttcaaaa gagcctctcc
     3361 gacaatcgca tccgtaacct tacctgcggt gattccgaga tcccgtaacg gagcatttaa
     3421 aatacggcgg ggcaatgtac agaacgctcc cacttcaccg atctcataac cttcaaaacc
     3481 actggacaga accgttactt tctctcctgc cttaggcgct gccgggatcg taccgatcgt
     3541 ggcctttgca ggatcatact gatactcctt attgctctca tttaccagct ttatgttgta
     3601 gtacagggta tctccgggct ctgccgcatt tcctcctgcc acggtaccgt tcagataacc
     3661 gatggcatcg gacagattga ttcttacctt gtccggggca ttgctgtgga tcgtaatggt
     3721 gatcgttgca tcataggtca ccctgccggt gccgctttcc caatgctgtt ccagttccgg
     3781 agttgtagta atggtaatac cttcggaaat ataatggaac actgcgccgt tctcattctg
     3841 ttcaaaagtt accgttcccg tgggatattc ataataatct actgcggata ccccgggaat
     3901 cctgtaatcg tatccgtcat aattgatcca ggtctctgtg ggtgtggtca cataccatac
     3961 cgtggcaatg gtgactccac tgaaggatcg accggtggtg agcaactgat ccataaaact
     4021 ttccgttaca ttttcaccat acaagacgcc gttgttgtca ttcacatact gttttacatc
     4081 ctcaagggtt tttccgcagt tttctttata ataggaagtg ccttcgctgt ctccaccgta
     4141 ataaacatag tgtaacaaat ctgcgggctt gcagtcagaa tatttgttta catctaatgc
     4201 tacgaatcca acgcactgcc agcttgcccc gttacttccg gttaactttc cttcggcatc
     4261 tacccctgta ttatccacag aaagatattt aaaggtttca gcatcatcca aagtcatctt
     4321 atgacctgca aacaatgaaa aatctccgtt tgccggcttg taattattgg gccatacgtt
     4381 ggggctggct acctgtcctc ccacatattt tgccatggtg cctccagcaa gaatcaccgt
     4441 tttgatggtg ggataggttt tctcttttac agcaacaacg ctgtcgtttc cgcttgcact
     4501 tgccatttca acaacatcgt tctgctcatt ctgcgttgct tcttccgcct gtaccgtaaa
     4561 tccggtgttg gagatgctgc cgatcatcag tgccgctacc agagtttttg ccagtttggt
     4621 ctgtattacg ttttttaact tcatttctgt ttcccctcaa aaccagtatt tctgttttta
     4681 ttacttcttt ttactctcct aataagtaag actacgccaa atggaaaaaa ggaccagaat
     4741 ttgaaaaatt tttcaagatt ctgatccttt tatactttta cagctaagat cggctgtcgt
     4801 tccgttcctg tttaatgttc ttttcgctta agaacaagcc atgctcccag actcgtcagg
     4861 gaaaaggcaa agcccagatt ccaggcatcg gagtcatcgc tggtctgcgg tcgtctggat
     4921 gctccgagga ctgctctgtc cgccaaggga actgtctcat cctcgatggt caccattctg
     4981 cggcgtcttg caccaagtac ctgcggggta tccgggattc caccggccgg tgtgccggga
     5041 tctgccgggg taccgggatc atctggtgta tcatccggag tatcgggttc atccggggtg
     5101 tcaggtgttg cggttggtgt tgctgttggt gtaggctcct cggttaccac cggtaccgtc
     5161 ggtgtcggcg taggctcctc ggttaccacc ggtaccgtcg gtgtnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnccaccg gtaccgtcgg tgtcggcgta ggctcctcgg
     5341 ttaccaccgg taccgtcggc gtaggcgtag gttccggctc atccggttcg cacggacagt
     5401 gaatctctcc accctgctta tacttatccg ctatgatgcc tccgacaaca gttccgccat
     5461 tatagacagt tgcattcgga gccaaaatat atccgaaaat agaatttaca ttaacttctg
     5521 ttgcattgcc aaagttccaa ataattctgt ctgcccacag ctgatgttcc aatgcttgct
     5581 tttttgcacc atgcgcattc atccagccgg taatattcac agttccggaa tcacttacat
     5641 ttaccacaac tgtttttcca taaaaagctg cgccggtgat ctctttgtca tagtcttcca
     5701 tcctggtatt taactcagca cttgttacat tcaataccag ttgtgtatcg ctgtcttcat
     5761 tcgccatagc atataaatct ttgcttttct gtaccatatt acctgccagg acattgctga
     5821 tctcagatgc aatatctgta tcccatacct cacagttctt cagaagattt ccagtatata
     5881 atttattttc gccaactata atattataac cctgtccagt cacttcccgc tcaacagagt
     5941 tctgggttcc gtcagaatta gtaaggggaa ttatcagttc cttttcgtct gttctgcctg
     6001 acgctgtata tttaagaacc tgattcggag cagaaagatc tcccagataa gattttcccc
     6061 catagcttac catggaaacc tttaattctt taattgcggc atttccctca atatggtcca
     6121 gatttgagga tacaaattca tttgtatata atacaaatga aaatggatca tttaaatccg
     6181 cacctgctgc ctgcacaata atcccctcgt tccctgcgac ggaacctgct accagtgctg
     6241 ctaccagaac ctttactact ttttccctta catacttgct aaatctgctc atctgatttc
     6301 tccctcaatg aaactgcgtg tttcaacgtc tttaaacgtt ttcgttcctc tttacaatta
     6361 ttaagactga cgccatttca aaaaaggttc agatttttat tatttttttg gtcttttcag
     6421 tatcattttt tatttttgta acacaagcct tagtatgcag cagtttctat caggactcca
     6481 tggatcagag cccgcctttt tgtaccggaa gaaccgtaac aggtggatag cgatacgatg
     6541 tttcctctgt cacttaagtc cgcatccgaa gcatacaggg aatgttccgt ggcccactgc
     6601 acataatcat cataggaggc atctgaggta aacgtggcaa aacggtcact ggtactcttt
     6661 gtttcataat aggaaaaaat ctcataaatg tagactttat cttccgtgta tatatagaag
     6721 taaggattct cctcgcagct tgccgtcccc ttcagcaaat ttttaaaagc cccaaacatg
     6781 ctctcatccc gcatattatg tccgaataca aaggtattcc tgtctgacca gtctgctgaa
     6841 gccccacagt ccatgaaaat gcatccagca ttcacactct ccccctcgaa ggaatgcttc
     6901 agataatagt cattgtcctc cccctgtacc accggataac tgatgtccaa tgccgggaaa
     6961 tagagccatc cacgaaaatc cgggttggtc ctgataagtt cggcaaagtc gatctgcagc
     7021 tccggataat ttttctccgc aatctcttcc tgtgtttccc ccgccgcagg ctctgccacc
     7081 acctcctggc ggatggcatc gtattcattt tttgctctct gctctgacag ataagaatgc
     7141 agactcattc ctccggttac cagtac
//
LOCUS       DAWDHD010000126         7136 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_616_length_7136_cov_9.604434, whole genome shotgun sequence.
ACCESSION   DAWDHD010000126 DAWDHD010000000
VERSION     DAWDHD010000126.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 7136)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7136)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7136
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_616_length_7136_cov_9.604434"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2142 a         1444 c         1883 g         1667 t
ORIGIN      
        1 caaagactac acgggaaaga ccgcgctccc gtgcagcacc tcctcagctt ccggactggg
       61 tgacagtggc aaccttttgg aagagatggc aggtaccggt gactggcagg aaggccacag
      121 atttagctcc ggtgcatacg aattttataa gatcctgatt atgaattcag ggaaaaagga
      181 atcttgaaac catacggcgg agagattcct tttgctgtaa taattgcttg taaaacctcc
      241 cggattgtgt caaactgata gttgtgacag gaatggattt tgagaaaaag tttccaaaat
      301 aaggagtaat catggctgag atattattgg gttcccatgt gggaatgtcg ggaaaagaga
      361 tgtttttagg atctgtgaag gaggcggcag agtacggtgc caatgcgctg atggtctaca
      421 ccggggcacc ccagaacacg agacgaaaag agataaagga tctgaatctg gaggcagggc
      481 ttgcctatgc agacaaagca ggcatcaaag aaatcgtgat tcatgctccc tatatcatta
      541 atctggggaa tatggagaag ccggaaaagt atgatttcgg cgtggaattt ctggcaaaag
      601 aagtaaaacg tgccgccgcc tttcacagta agatactggt actgcatccg ggttcttccc
      661 tgagtgccgg agtggaagca tccgtggagc agatcgcaaa gggactgaat ctggtgcttg
      721 atgcggatga cagtgaggtc aatatcgcac tggaaaccat ggcaggcaag ggcagtgagg
      781 tcggcagaac tttcgaagag ctgcgaaaga tctatgacag agtagagcgg aaggacagac
      841 tgcgggtatg ctttgatacc tgccatgtga acgatgctgg atatgatctg ctgaatgatt
      901 acgaaggtgt gcttgcgcag tttgacaaga ttctgggact ggaccagatc gcggtgatcc
      961 atgtgaatga cagcctcaat cccttaggct cccacaagga ccgccatgcc aatatcggtc
     1021 agggcatgat tggctacgat acgctgcatc ggatcgtcca tgacgagcgg tttgcaggtg
     1081 taccgaagat tttggagaca ccatggctct gcgcagaggg agaaacgaag aagacgattc
     1141 ctccttacaa gcaggagatc acatggctgt tgcaatagag cggataattc cggaagcttg
     1201 gacgagaacc ggctgtaaag tttaaaaatt tcataagatg atttacacat tggatccggg
     1261 cggagatttc tgctcggatt tttgattaaa ttagctagaa tagattaaat taatcaaaat
     1321 taagttgttt aattaggcaa aatacacatt aataaaaact aaattcgtaa attagcaaca
     1381 atgtacaaaa ttcagaacat ttgctatcct tgaattagac agagaatata ggaaggcgcc
     1441 cctgtattct cggaaaatat taaggagaga aggtaagaaa tgaaaaacaa gcttagagta
     1501 ggagccttgt tattaacagc agtcatgctg gtctccggtg tctatgaatc ggcacctatt
     1561 gtggttttgg cagaggaaca gacagaaatt gaacaggaag cattacagac cgtaaatggt
     1621 gaaaatattg aagaaaagat tgcagttcgg tcagcagata cttccgaaat ggtacaggtt
     1681 tatggagaag atattgaatt tgacaaaact aaggactggg atgatcatgg agaatttaac
     1741 attaacttga ccaccgaaga ggcattgaac agcggggcaa aggtgtccat ggatgttctg
     1801 gtaccggaga atgccgcata cagcggtatt attaaaatcc agggtgtggc aagagtcgga
     1861 agcaattgga cctggactga ggcagacagt attacagaac ttacgccgga aagctttggg
     1921 gaagcggaga atgagtatcg taaggcggct gtgacattta cctttggagc aggtgtagag
     1981 gcagatgcac tggcacagtt tacattgaaa tttgcaggat atcaatgtga ttattccggt
     2041 gcgatctata tagccaatgt aactctcact gatggagaaa agcaggagaa cggtggaaac
     2101 cagggcgaga ctacgaatat cccggtggca gaaaaaacgg acagacagct ggtatacggt
     2161 aaagaagttt cttttgatgc ttctaaggac tggagtgatc agggagagtt tgagattggc
     2221 attactacag aagaggcact ccataatggg gcatatctga ctatggatgt attggtaccg
     2281 gataaagttg agttctccaa ctgtatcaaa gtgcagggca ttgccaaagt aggcgatgag
     2341 tggacatgga cggaggccgg taacatcccg gaacttacga taggcagttt tggagaagca
     2401 gtggacggct atcgtagtgc aaccatccgt tttgattttg gcgataatat cgagaacggt
     2461 gcggtaaaga ctacactcat taaactggca ggataccagt gtgattatgc cggccctatc
     2521 tatattgaga acgtcaaatt atacgacaaa cagacatccg gtgagcagcc ttcacaaccg
     2581 gagaagcctg cccttcctga gaaggatccc agtatcatcg ataattttga ggatggcacc
     2641 tgcgacgact gggctaacgg cggtgcatgg cagtatacca acagcattgt aaattctgtg
     2701 gcagaataca acagttcgaa aatgatgaag gtagatctgg actataccgg tatgggcagc
     2761 tacagttgga gcgaggcaaa gctggaaaac aaatttgcga aaagttttga catttccgca
     2821 tataaccttc ttacttttga ctattattat ccggcagcaa tgtccggaag taagatcaag
     2881 atcttctcca atgccggaat caacaaagag gcagagatta ccgaggtaga gactctgtca
     2941 gacggaatga ttcatgccaa agcacaggtg agattctctc cgactgacaa gggactggca
     3001 gatctgacca tcggctttgt aggagtgaat acggaattta agggaagtgt ctatgtggat
     3061 aatattacct taagccagta caatgcgaca ggagattttg tgaagatcac agcagctccc
     3121 aatgctgtcg gtacccaggc agatgtttcc aatgctccca cacaggtaaa actttcggat
     3181 gcaaatgcag gtacttccgc aactgcattg tatgcctatc tgatggcgct gcgggcgaaa
     3241 gatcaggtat tgttcggaca tgaaaatgac atcaacaaaa tggtcagcag cacggccagg
     3301 gagggcgatg tcaaggagat taccggaagc atcagcggtg tctatggtat cgacagtctg
     3361 gcactgaccg gagcagagct tggcatttcg gatgcgaaag aggctttgaa tcaggcagtt
     3421 gcctacagta agcaggcagc aggacaggga gccatcataa ccctttccac gcatatgccg
     3481 aatttcacca atgagaagat taaggtgaat gcggatggaa gctatgactt tacagcctgt
     3541 gatttctctg aggcaaagga tacttccaac aactgtgcgg agcagattct gcccggcggt
     3601 gcctataacg atcgctttac agcatacctg gatattattg ccgaatatgc aaatgcactg
     3661 caggaggaaa atattcccgt tctattcaga ccttatcatg agaataccgg aagctggttc
     3721 tggtggggat cttccaccgg tgtggagact tataagagcc tgtggcgtta tacgcaggaa
     3781 tatctccaga gtaagggtgt ccacaacatg atctatgtat attcacctaa cggtcctgta
     3841 accggagcgg aagagtatct gacccgttat cccggggatg aatatgtgga tgtgctggca
     3901 tttgactatt atgatgatta cagtacctat cctgcaacct ttgatgaaag cttcttcaag
     3961 aacctggaga ccacctgtca ggttgtaagc ggtctggctg cggaaaaagg gaaggtggca
     4021 gcaatctccg agaccggcgt ccgtgccatg aaaaaggacg gctccgatta cgaaggtctg
     4081 ttggtatccg gcaatcccat cgtgggacac aactggtatc aggaggttag caatattgct
     4141 gcaaaatgtg acatgcctta ctggctcgta tgggcaaact ttagtgatac caatttctat
     4201 gtaccctata agtacaatga tacctacggt caggaactga tcaatgaatt tattgcattc
     4261 tataacagta acaacagtat cttcggtaac ggaaccaatt tctatcgtaa aatgggaacc
     4321 gtgaatggca cggcctataa caccgcacag ggctacctga tcgctccttt tgaactggct
     4381 gccattaagg aggagactgt atttcgtgcc agcgtgaaaa acgcacagaa agtaggtttt
     4441 gttgtgaaga atccggcaaa cggcaaagag attatcgtgg aagctgcgaa ggtggctgac
     4501 agcagcatct gcgaatatgc ggcgactttc accgcacaga tgctgtcaga gatcggacag
     4561 gcagatactg ctactgtatc tttagtggca gacggcaatg tacttgctac cgtaggaaat
     4621 atcagtttag ggaaagacaa ggataaggca gcagccaatg tcatcgacaa ctttgactat
     4681 tatgcaggca gcgatggcct gttgcagagt gcttatacgg agaattctgc cggaggatgc
     4741 agttcctcct ttacattaag cgctgatcat aaggcagacg gtgtctacgg tggtgcatta
     4801 cactatcagt tgaatacggc aaagagtgaa gtatggaccg gacagaataa gacactggaa
     4861 gatcttgacc tttctgcata caatgctatc accatgtggg tagatccgga cggtatggga
     4921 cagaagctgg taatccagct ggcagatgat tccggtgagg agtttgaagt atatttaagt
     4981 gattttgtaa aaggaacaga agcaagctat gtgaccattc cgttcagtgc tttcaagggg
     5041 aaaaagagcg gaaccttaaa taccgcgaag atcacacggt tcgccatctg gtgtaattcc
     5101 atcattccgg aaggatatac cggagactgg aaagtggatt ccgtgattta ttttgatggt
     5161 attgaggcat ttactgcaga tgatgcactt ctggcacagg cagatgctga cggactgata
     5221 tttacaaaag aatctcctat caaaaaagga gagaatcagc cggaaggacc gaaacaggat
     5281 actccgaagg aagacaacac aaatacagag aataatacgg caggtacttc cggtagcagt
     5341 aattcttcac aggtggaaaa tacagtgaac tggtcggctg caaaagagca gattacagcg
     5401 aaggcagcaa acggtgcagc agaaaatctg aattttgtaa gtaccggtga gacaaaaatt
     5461 cctgtagagg tgttgagaag cattcaggga tccaatgtga cattggctct ccataacgga
     5521 aatggagtcg caatgagtat cagtggacag gatctgaaaa atgtagattt ttccaaaatt
     5581 cagaacatcg atctgacagt tacaaaagat ggcgactgca ttaccgtaag agataccggg
     5641 aactttgcag tacccgtcaa tatgcatatc agcatgggtt cagagaatgc aagaaagtat
     5701 gcaaatctgt atcgtaggaa tgccgaaaac ggacagtggg agttctgtgg ttctttccgg
     5761 atcacagaga acggacaggc gatgttcgcg ttgatctgcg gaggagagta tcgcacgagt
     5821 gttactatag aagccgtaaa ggaaaccgta gggcttcatg gcatcggtta caaggtgaaa
     5881 gcaggtgaca cgcttactaa gattgcagca aaacatagga tttcgttggc agaactaata
     5941 cgtaagaatc ctcagatcaa gaatatcaat aagatcagac ctggacagac tataagattt
     6001 tagcctgaat taaagaaaat attcttaaaa tgataaaata aattccgtac aaaaatcttg
     6061 atttagaaga tattttgtac ggaatttatt tattccttta atatttcttc cagcaggacg
     6121 gcagcatgat caccagcatg gggaagagtt ccagtcgtcc ggccagcatg tcgaacatca
     6181 gcacacattt ggacagagga ctgaataatg caaagttctg ggtggggccg accagctcca
     6241 gtccgggtcc gatattgttc agggttgcca gtactgcagt aaagttcgtg gtcagatcgt
     6301 attcgtccag gctgacgatc agcagagaga cgaagagcac gataaaataa gccgtcatat
     6361 agacattggt ggagcgcaac gtctcgtgag agaggttgtg cccgtccatc ttgatcttct
     6421 tgaccatacg gggatgtatc atcatggaca gttccttgcg gatcgcctta aaaaggatca
     6481 gcacacggga gaccttcata ccgccgccgg tactgccggc gcaggcaccg atcatcataa
     6541 gtaacagcag gatcgtcttc gataattgcg gccacatgtt aaagtcagtg gtggcaaatc
     6601 cggtggtggt aatgatcgtt cccacctgga agaaggagtg ccgcagggtc tctcccaatg
     6661 ttgcatagag aggacggaca ttccaggcaa tcgtcagcgc actgaccaga ataatcacaa
     6721 aataccagcg gacctcttcg atggaaaagg cttccttaaa ctgcttactg agcaggcaga
     6781 aatatatggt gtaattgaca ccgcttaaga tcatcagtac cgtcaccgca ttctggatca
     6841 acggggaata accgccgata ctgctgttct tgataccgaa gccgccggtg ccgacgctgc
     6901 cgaaggtggt gcacagggaa tcaaataacg gcatgccgca cagacacagg gcgatcacac
     6961 ctaagatagt aatggcaaga taaattccat agaggatctt cgccgtatcc cggactctcg
     7021 gtaccagctt gctgacggaa ggccccgggc tctccgcacg catcaggttc atggtagaac
     7081 cccccatcat cggcaggatc gccataataa aaaagaatac gcccaaaccg ccgatg
//
LOCUS       DAWDHD010000127         7076 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_621_length_7076_cov_10.190571, whole genome shotgun sequence.
ACCESSION   DAWDHD010000127 DAWDHD010000000
VERSION     DAWDHD010000127.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 7076)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7076)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7076
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_621_length_7076_cov_10.190571"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1772 a         1836 c         1653 g         1815 t
ORIGIN      
        1 agttcacgat cacagagccc tctctctcgg agataccgca cagacttccc atgttctcaa
       61 gctgtcccca ggtgctgtcg gtccagatat aacgatcggt aaattcatta cggtccgcct
      121 tacaggactc cttgaaccag ggatgtgcca cggaggtatg tcccggcacc aggtctaaca
      181 ggatatgcat gtcccgcttg tgtacttcgt gaaacagacg cttcagatcc gcattggtgc
      241 cgtatctggg cgctgcctga tagtagtccg ccacatcata gcctgcgtct ccgaagggtg
      301 atgcaaaaca ggggttcatc cagatcgcat tgcatcccag ttccttaata taatccagtt
      361 tttctatgat accctgaaaa tcgccgatac cgtcaccgtt ggtatccaga aaagactggg
      421 gataaatttc ataaaagatt gcattatcaa gccactttgc catagtgtac tccttcctat
      481 atgctttatt tttattgtaa aaacgttttc cgttactttc ttttaacata ccacggaatg
      541 gctacttgta caactgcgaa aaaacatcta taataaagta gcttgatgcc ccgatatgcg
      601 cataaaaggg aagcgtaagc ttcacgatat gcgaatagcg gacagggtgc tatgtgccag
      661 tggcacatgt ttagcacgga tcgaagcgga gcgtagaagt gagagtgcgt agcacaaaac
      721 aatccgcagg catccaacta ggataaaata agaacgaggt ctatcatgtt aacacagaaa
      781 gtaagcacca tcgatgaact cctgcagttc gtcagtacgt tacgccaaaa gcacggcgtc
      841 cgcctctggt tccgcgggga agagaatgcg gatctgactc tgatcccttc catccaacga
      901 agccagaaaa gactggactc cgaacgctat attgccaatg atttctatat ccgggcgaga
      961 cagatcctgg acaatcctcc cgacaaacat aattacgccg gctgggtctc cctgatgcag
     1021 cactacggtc tgcccacgag aatgttagac tggacccagt ctcccctgat cgccgtattt
     1081 ttcgccacgg aaacttacag agagactccg gacaccgatg cctgtgtctg ggtactaaca
     1141 ccggggctgt tgaacgagaa ggaaggattc ggcaactgca tctatcccat tgatgcggac
     1201 accacacagg aaatgctgct gcctgccttc aaacacaacc accacaatcc ggaattgaaa
     1261 aacaagatcc tggcttgcag ctccacagag aataacctcc gcatgtactc ccagtactcc
     1321 aacttcaccg tacataactc tctggaacgt ctggaagata tctgcgatga aaatatgctg
     1381 tataagatca ttatcccctc cgggcggaaa caatacttta ttgaaagcct gcgcgtattc
     1441 ggcatctccg agagcttcgt atatcccgat ctggatcata tatccagtga tttgaaagat
     1501 tcctatggaa tttaataatc atgctaccta cagattgttc cgcactacgt gctctcacaa
     1561 tcctgcccaa tattcgcata tcgtgggatt gacatcccac atttatgctc atatcggggc
     1621 gggtcccaag ttttttcgcc cacctatgag caagctcatg tgggcttaaa ccttttgacc
     1681 cttgtatcat atatattaag aaaggaaata tacacatgaa aatcgtatta cagcatctga
     1741 ccaagaaatt ccccgcccgc ggtcctgtcc gcggcaggaa gaaccgcgaa gatgtcattg
     1801 ccgtgaatga cttcgacttc gagatccccg acggcaagct gatcggactt ttaggtccct
     1861 ctggctgtgg caaaagtacg gcactgaatc tgatctgcgg tctgctgaag cccaccgagg
     1921 gcaagatctt tttcggagaa gatgatgtca ccggacttcc tcccgagaac cgcggtgtcg
     1981 gcatggtctt ccagaactat gccctctacc cccatctcac cgtggagaaa aatatccagt
     2041 tccccttaga gaacctgaag ggtgccgaca aactttccaa agaggaaatg agcaagcgag
     2101 tgctggaagc ggcaaagctg gtacagatcg accacctgtt agaacgcaag cccaacgagc
     2161 tctccggcgg tcagcagcag cgtgtggcca ttgccagagc cttggtcaag ctccccagag
     2221 tcctgctttt ggacgagcct ctttccaatc tggatgccag acttcgtctc cagaccagag
     2281 aagagatccg ccggatccag caggagacgc agattaccac catcttcgtt acccacgatc
     2341 aggacgaagc catgagcatc tccgatatga tcgtcgtaat gaaggacggt atcgtgcagc
     2401 agatcggcaa gccccagaac gtatacgacg atcccgtgaa cctgttcgtt gcgaagttcc
     2461 tcggcacacc tcccatcaat gtctttgacg gtgaagtgaa gagcggacag ttatacatcg
     2521 gctccgaagc tgttctgcct gctcccgggg tagcggatca gactgtgcat gtcggcattc
     2581 gtccggaagg ttttgtatta aaagaggacg gaccgctctc ctgcgatctg gtgcgtctgg
     2641 aggtcatggg ccgggatgtg agcattgttt ccacccatga aaagtcccag aacgctacca
     2701 tccgttccat tattgatgcc gacggaggga aatctcttcc caccggaaaa gtgcatttcg
     2761 ccttaaaacc cgcaaaagta ttcctctttt gcaccgacac cgaagaacgc atccgctttt
     2821 aatattttat ccgattctgt agtccggcgc tattcatggg ttgcagaaag aattgaggtt
     2881 tcttatggaa aaacaaaatc tgaaagcctg gctctatcta ttgccggcat tcctgtttct
     2941 gggtgctttt atgatctatc ctcttatcga cgtattcgtg tactcctttg aagagggtta
     3001 caattccgca tcccagacct tttacggcgt aggcatgtac aattactcct atgtgctcca
     3061 cgatccgtac tttttacagg cattgaaaaa tacctttatt ctggtcatca tcaccgttcc
     3121 ggtctccacc ggactggcgc ttttgatctc cgccgcctta agctccatca aaccgctgcg
     3181 ggatctgttc cagaccatat ttttcctgcc ctatgtgacc aatactctgg cggtcggtct
     3241 ggtcttcatg attcttttca aaaagacccc ttactccgac ggtcttatca atctgctgat
     3301 ccagtttttc ggcggcagct ccgtggattt catcgacgga ccctactggg cgaaaatgtt
     3361 cgtgctgtgc ttttacacca tctggatcgt tatgcctttt aagattctga tcctgaccag
     3421 tgctctcgcc agtgtgaacc ccatgtacta caatgcggca agagtggacg gcacttcccg
     3481 tttccggatg ttcaccaaga tcacactgcc catgatctcc cccatggtat tctacctgat
     3541 catcaccggc tttatcggcg cattcaaagc ctacagtgat gccgtggcgc tgttcggcac
     3601 gaacttaaat gccgcggaga tgaataccat tgtcggctac gtctacgata tgctctacgg
     3661 tgacagcggc ggctatcctt cctatgcttc cgcagcggcg atcctgctgt ttgccatcgt
     3721 cctgaccatc acctgcatca atctgctggt cagcaaaaag catgtccact atgtataaga
     3781 aaggaggtct ttgtgatgtc tgatgttaca tctgattatg aaaaaatcga gaaacgcacc
     3841 cggatccgcg gcaatatccg caatgggatc gtctactttt ttctgtgtct gtgggcactg
     3901 atcgtactgt tcccttttta ctggatggta ctgacctccg taaaaagcta cggctcctat
     3961 aatgcggaat atattccgaa gtttttcacc ctttctccca ctttgcagaa ctacgtggac
     4021 gcttttacca ctgtttctct gggaagatac ctgtggaaca ctttgttttt caccgtggtc
     4081 accactgcca tcatgctggt ggtcatcacc ctggctgcct tcgcttttgc aaggttgaac
     4141 tttgccggaa aagatctgat ctttacgtta tttttatctc tgatgatgat tccgaacgag
     4201 ctggtggtca ttacgaattt caccactatc acgaacctgg atctgcgcaa taccttcacc
     4261 ggtctgatcc tgccttccgt gaccagcgta ttttatatct atctgctgcg ggagaatttc
     4321 gcacagattc cggacgagct gtattatgcc gccaaggtag acggtacttc cgatctgcga
     4381 tatctgcgca aggtcatggt gcccatctgc aagcccacgc tgatcaccat cgtgatccta
     4441 aaggttattg aatgctggaa ctcctatgtg tggcccagac tgatcaccga tgatccggat
     4501 tactatctgg tatccaacgg tatccaggag atccgtgaga atggtttcgg cagagagaac
     4561 atccccgcca tgatggctgc ggtggtcgtg atttccgttc ctctggtggt gttgttcctc
     4621 gtattccgta aaaaagtcat ggcaggcgtt gccagaggag gtaccaaagg atgagtcgat
     4681 tcagcaaaaa actggcatta ttatgtgccg tgggcactct gtctctgtcc ctgaccggct
     4741 gccatggttc gaaaggcctg ccggaattca ctgttcctga ggagttcgat acttccagaa
     4801 actatgagat caccttctgg gcgaaaaacg ataccaacaa aacccagacg gaaatctaca
     4861 agaaagccat tgcggatttt gaagccctgt atcccaatat cacggtgaac ctgaatctat
     4921 acacggatta cggcaaaatt tacaacgatg tcattaccaa tatttccacc aatacgacac
     4981 ccaatgtctg tatcacctac ccggatcaca ttgccaccta cctcaccggg cagaatactg
     5041 tagttcctct ggatgacctg tttgccgatg aaaaatacgg tctgggcggc acagatcttg
     5101 cctatgacgg tcctgccaga gaagaagtca ttcctcagtt tttgaatgaa tgctccatcg
     5161 acggtcatta ctatgccgtt ccttacatgc gttccaccga ggcctgctac gtgaataaga
     5221 cctacgtgga aaatcttgga tatactctgc cggatgtact gacctgggac ttcatctggg
     5281 aagtctccga ggctgccacc gcccaaaatg cggacggcac ctatgtggtc aacgggcaga
     5341 atgtactgat cccatttatc tacaagtcca ccgacaacat gatgatccag atgttaaagc
     5401 aaaagaatgc gggatattcc acggctgacg gcagcattga acttttcaac gatactacca
     5461 cggatctcct gtatggtatt gccggacatg taaaaagcgg tgccttttcc accttcaaga
     5521 tctccggtta tcctgcgaac ttcctgaatg ccgggcaatg tatttttgcc attgattcca
     5581 ccgccggagc cacctggatg ggtacggacg ctccgctgtc ggatatcagt gaggactctc
     5641 tggtgcagtt tgagaccgcc gtgcggatga ttccccagtt tgacccggag catcccgaaa
     5701 tgatctctca gggtccttcc gtctgcgtct tcaataaatc agactctcag gaagtactgg
     5761 catcctggct gtttgcccag tatctgctca ccaacgatgt gcagattgct tattccgaga
     5821 cagaaggcta tgtacctgta acctctaagg cacagaactt cgcagagtat caggagtatc
     5881 tgtccgaatg cggtgcggat aacagtacgc attatgcggt gaaaattgag gcttccaaac
     5941 tgttgcttga caacgtagac aacaccttcg tcacaccggt atttaacggt tccgcatcct
     6001 tacgtgatgc ctccggacag ttgattgaaa acgtggtgaa gtccactcgc agaaaagaga
     6061 ctgtggatga tacctacatg caaaagcttt acagcgatgt agaaagcctg taccgtctcg
     6121 gtcagggaag tgatgcctcc ttcggcaaaa aggaactggg tgccatgccc cgggcttccg
     6181 tgatcctgtt aagcacactt gcagtcacct tggttctgat cctcatgtat gtagggcgag
     6241 aatatttaaa aaacagaaaa aagtaatttt tcagccccat tcgtaagata atatctggtg
     6301 gtattatcct gcgcatgggg ctctttttgc aaaaaatctt gatttatctt gcatttcagg
     6361 tataatggaa ctgttcggtg tctgaatgcg ccggataaat tgaggagatg tctgtgtatc
     6421 tgcacagacc catatgcgcc acaagcgcag gaatggagga ttattatgaa aaagaagaca
     6481 cttgcattaa ccatggctat ggctctggtt ctgtctctcg ctgcatgtgg aaacagcaat
     6541 gcagacgttg ctgctgaatc taccgctgct gccaccaccg aggctactac taccgaagct
     6601 gctactaccg aagccgctgc tacagaagct tctaccgagg ctgctgaagc tgttgctgat
     6661 gagaagtccg agggtgttat gacctatgaa gagtacatgg ctgctgatct cgattccgaa
     6721 gttgtgatcg aggcttatgt tcaggctaaa cagtcctggt gggaagacaa ggctaccctg
     6781 tacacccagg atcaggacgg tgcttacttc atttacaatt ctgtatgttc cgaagaggat
     6841 tatgcaaagc tggtacccgg taccaagatc aaagtaaccg gttataagac cgagtggtcc
     6901 ggtgaagtag agatcgctga aggtgccacc ttcgagatcg aagagggcag ctacatcgct
     6961 cccgttaccg atgtgaccga tctgttaggc accgatgatc tgatcaacta ccagaaccag
     7021 ttcgtagctt tcaagggtat gactgtagaa gctgcaggtc aggatgctga cggcaa
//
LOCUS       DAWDHD010000128         7053 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_623_length_7053_cov_13.545584, whole genome shotgun sequence.
ACCESSION   DAWDHD010000128 DAWDHD010000000
VERSION     DAWDHD010000128.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 7053)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7053)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7053
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_623_length_7053_cov_13.545584"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1826 a         1708 c         1446 g         2073 t
ORIGIN      
        1 catcaatcag gtcgttgctg aactgaccaa ataatatgtg cggtgcatta tcagcaaaca
       61 ccaccctgta tcttaagata taatgaacgc aagtgagcca atatgcttgc ataattggcg
      121 aacggcgaat ttgatcatga tcgttgcgaa gcaacggtaa gcaccgcagg tgcgaatcat
      181 gatattatat gcaaaagcat aaatttgcgg attttggagt cgagggtgcg gacattttct
      241 tggactatct aagtgaccaa tccaagtcta tgtctgtact ctataggact caaatatcct
      301 aatgattttt ttattctttt tcattatacc aataaagata atcatttaaa ataccaataa
      361 attctgagat gtttacccca gaccaatctg cattatagaa catctcattt ttaattcttc
      421 caaatacgcc ctcacaggca gaattatcag gtgaacagcc tttctttgac attgatcgta
      481 tgaaaccatt tttctccatc ctgtcaatcc agcctggcca gcggtagtgt acgcctctgt
      541 ctgaatgtat gattggtttt tctcctacag atagtaattt agctgcatcg tcaagcatag
      601 aattgacaag taatgcatct ggagaagtgc ctatattcca attgacaagt aagccatcaa
      661 aacaatctac tataggcgat aaataaacct ttcctgccgg aattgcaaac tcggttatat
      721 ctgttaacca cttttcgtta ggtttagatg cagaaaaatt tctgttaatt agattatcta
      781 ctgctggaga tatttcacct tgataggaat tatatttacg agttcgtttg atttttacta
      841 caagttgttc ttctttcata atcctacgaa taactttttc tgagacaatt atattttcac
      901 gcttaagtag ggcgtgaatt cgtctgtaac catatcgacc tttattctct gtaaataatt
      961 caatgactct aacacgtaaa agtgtatatt tatcttcttt ttgtagggat tgttcctgat
     1021 aataataact gctttttgat aattgcaatt tctgtaataa taatggcagt gagtatctat
     1081 ttttcaagac atcgattatc accgccttct ctctgttgct gagagcactc tgatcgatgt
     1141 ctgggtcttt ttttaataca tttatcgttt ctttaagaat gtctatttct aattgcatat
     1201 cttgcatttg agccttcaac tgatttattt catcagaaga aataactgta cttgaaacag
     1261 aaccttctac taatgttccc ggagtaatgt ttttgtgatt cataagacct aatgtacctt
     1321 cttttaaata acgttttcgc cattgataaa tactagctct actatatcca atgtcttctg
     1381 aaacatattt tatattttct ccgagctcat agcaacggtg aattgcatct aattttactt
     1441 ctaatggagg attgcgagga tgatcaggag gattatcaat tacaggggat tctttccttg
     1501 ttttgggtgg ggtattttcc tcagcaatcc atgtgtacag atttctgcgt gttggatacc
     1561 ctagaatcct aatagtttca gttacagact ctgtttgatg atatatccgt agtgcaatat
     1621 ccttttgttc ttttgaatac ataaaaaggc tcctttcgaa gccctatagt agtccagaat
     1681 tttgtccgca ctctcgtctg tgaaagtttt tctgtaaata agtattagct aataggtctt
     1741 ctatgataaa atctaaatca taggaggcct attattatgg caagagaaaa gaaacctgtt
     1801 cacaaagtac aaatgaccga aggaaaacgg aatatcattc atcagctttt ggacgaatac
     1861 gatatccagt ctgctgaaga tattcaggaa gctctcaaag atctcctggg tggcacaatc
     1921 aaagaaatga tggaagccga aatggatgat caccttggat atgaaaaatc ccagcgttcc
     1981 gatagtgatg attaccggaa tggttacaaa accaaacgtg tcaacagcag ttatggcact
     2041 atggagattg aggtgccaca ggatcgcaga tcctcttttg agcctaaggt tgttcaaaag
     2101 cgtcagaaag atatttccga tattgacagt aagatcatat ccatgtatgc aaaaggaatg
     2161 actaccagac agatatctga aacgattgaa gatatctatg gttttgacgt gtcagaaggg
     2221 tttatttccg acgtgacaga caagctgttg cctcagattg aagactggca gaaccgccct
     2281 ctagatgaaa tctatccgat cgtctacatt gatgcaatcc attattctgt acgtgataat
     2341 ggggtgattc ggaaacttgc tgcatatgtc attcttggta tcaatacaga aggaaaaaag
     2401 gaagtacttt ccatcaatgt aggcgataac gaaagtgcaa agtactggct ttctgtttta
     2461 aatgagttaa aaaatcgcgg agtaagggat atccttattc tctgtgctga tggacttaca
     2521 ggaatcaagg aagcaattgc tgctgcattc cccaagacag aataccagcg gtgtattgtc
     2581 catcaggtaa gaaacactct gaaatacgtc cctgacaaag acaggaaggc ttttgccaca
     2641 gacctaaaaa ccatctatca tgcttcagat gaatcgaaag ccagagaggc actggagaga
     2701 gtcaatgaaa aatggtctcc gaaatacccc aactccatga aacgctggta cgacaactgg
     2761 gatgctgttt cgcctatttt taagttttca gcagcggtcc gaaaggtcat ttacacgacg
     2821 aattccattg aatcgttgaa tgcaacatac cgcaaactga accgccagag gagcgtattt
     2881 ccgagtgata ctgcactgtt aaaggcattg tatctgtcca cttttgaagc taccaagaaa
     2941 tggactacaa cgattcgaaa ctgggcgcag gtatatggag aactgagtag catgtacgag
     3001 ggacgccttc cggaataata ccatgaaaca cagggaaaca gacctgtatt cttgacatac
     3061 aatcatttcg ctgttatata taaaaacaag gcggaaagct tattcacaag ccttccgcca
     3121 ttcattatca tagaagatct acgtatttac agagttttct tcacacactc ggattttgaa
     3181 atccatcaac aaacggcttc cgggagatct ccgaaagccg tttgttatct aaagcattat
     3241 tccactacta ccgtaccgtc ttcttttacg gtaatattca tgtcatcctt cacatattcc
     3301 aggaactctt cccccagact gtcaatgaca ggcatctgca cattgtccag ccatacgtcc
     3361 gccaggattg ctccggctgc tgccagggaa tcaataggtt cggaaaacag catacatgcc
     3421 ggctgtctgt tcatggagca ggcacagtaa agtaccagtc cgccggtggt agatcctatg
     3481 gtcgtcggca ggcacagggc tttgcccgcc atctgtttac catagaggtc gggattattc
     3541 tgatctccgc aggttgcttt tttatcaccg aactgtaatg ccttctggaa agatgccagt
     3601 gtgttcagac ccccgtgaga taccagtgcc ttggctgtta cagttccgga ggctacgatt
     3661 cttcctttaa attctttcat ctgtccgctc ctcccttcgt aatctgctct aagatctcag
     3721 catcggtata atatcttgcg ctggtatagg tacgcagctt gttgctggaa gtaatgaccg
     3781 gcatcttctc gctgagagga ttattcatat acattaacgg gcagatatag gaagtaatga
     3841 ctcccgttgc ttttaatgtc tccgcataag gggtctcctg aaattttttc agtaccgcag
     3901 gagccgctgt gaatacggta ggaatacaca ctctggttct accggcttct ttcaggctct
     3961 gagagacttt ttccgtccag tcgatgagct gctgtaaact catatgggga catcccatga
     4021 aacacagctt cggcttggca tccttctttt tccagatcac cggatagctc tcatagaccc
     4081 tctgaagttc cgcatcatct accacgtaga cctttgcatt ctccgcgatc agatcttttc
     4141 cgtattttac agcctccgga gtgatatttt ccacatggta cagacctacc gcaccgttgg
     4201 aagcagtcgc tgcgccgaag tctttcagat aggtctttgc tgcatcatcc agttcaccgc
     4261 ccagccactt gtccagtcct acgatatagg gaacatccgc catcactttc ataccgatgg
     4321 cagatccaag cagctgtgct tccggctttt tcgtggtctc gatcttcacg atccaggtgg
     4381 cttttctgcc ctcatccgtt aacagaccga aacggggcac atagcccact accgagccca
     4441 tgaggtcaat gataccggag ttacgattgc atcttgctcc caatacggaa ttggcataga
     4501 ccactgcaga agactcagac caggacagca cctctcccat ggccggggta tttcccactt
     4561 catccatgta acaggtgcag gtgtaagcat ccctttccat gatacccagc tgtgtcagct
     4621 gtttctcgta gtcatcctgc ttggagtaca tgaaattctt gaacacaaaa tcctgtaaga
     4681 aagaacttgg cacattggga tccagtggtc tcggatcggc ggaaaatttc tgtttggata
     4741 cattgccgga ttcgatcagc tgattcatca gttcatacac cggcgccagt gctttcaatc
     4801 cgaaagaggt taccagatgg ttgtatttac tggtcacagg caccataccg tccgcaccga
     4861 acagctcgcc gtagcgcacc agcgtctgca tgaccttcgc cagggtcgct ccctgttttc
     4921 catcatatat ttcctgttgt tcttttgtta agttcattga tttccctccc tttaaatctt
     4981 catggcaact tcataagcgc gccatacgac acttcgcaga ttgcctacac cgtcacagtc
     5041 acctaccagg ctgacattgc tgcccttcgg tgccagtgga ttgggaatgt atcctgcgga
     5101 gctgatcaca ctgtcacagg gaattgtgat ctcttttccg gaagcatcct tgcatacgat
     5161 gctgtcatcc tttacttcct gcagcgtagt ttccagatat acgggtactt tcttccaggc
     5221 gaaccactct ctcagatagg aactgttggc caggcatact ccggtctggg cgatgagatc
     5281 gtttttcatc tctacgatta caggctgtct gccctgcaac gccagttcat aagcaatctc
     5341 gcagccggtg agtccaccac cgatgacggc aaccttttct cctacgggag taccggtaag
     5401 gtattcgcat gcctcaacca tcttctcatg gccgggcact tttttcagta ctctgggcac
     5461 tgctccggtg gcaacgatca caggacttcc ttcaaactgc tcgatatttt ccactttttc
     5521 attgtaatgg atctcaatct tcagatctgc catctgcttc cgataccagg ttaacagatc
     5581 ccgcagtttt cccttgtagc tttccgcgct ggcggcaata aaggttccgc ccagatggtc
     5641 tgtctgctcg tgaatcaccg gctcatgtcc ccgcagtttc agtactctgg cagcctccat
     5701 gccgccgata cctccaccga taatatgtac cttcttcggg ctggtggtcg ggacgatctt
     5761 gtgcttctcc cactgcatgg tctcggcatt taccgcacag cgtgccagat gcagagaatc
     5821 agataatgcc tgatcattgg gaactccctt atagtgacac atgttgaagc agccgttgtg
     5881 gcacagaata cagggacgga tgtcgtcctc cttatcattc atcagcttgg tcacccattc
     5941 ctggtctgcc aggaactgtc tggcgaatcc ggcaccatcg attcttccgt ctgcaatagc
     6001 agccgcagcc gtctctggat ccatacgtcc ggcgcatacc acagggatat ccacgaattt
     6061 acggatatgc gccacatcct ccagattgca gttctccggc atatagatag ggggatgtgc
     6121 ccagtaccag gcatcatagg ttccgttatc acagttcagc atatcataac cggcatcctg
     6181 cagatacttt gctgcttttt cggattctgc catatcacgg cctgcttctg tgaagctttc
     6241 tccgggaagt gccccctcac ggaacccctt ggtcttggat tccacgctgt acctaagaga
     6301 cacagggaaa tcctgcccgc agactttctt gatctctttt acgatctcta cagcaaaacg
     6361 atatctgttt tcaaaagatc caccatattc atcggttcgc tgattcacat attttaaagt
     6421 aaactggtcc agcagatagc cttcatgtac cgcatggatc tccacaccgt ccacaccggc
     6481 atcctttaat aattttgctg tcttgccgaa tgcagtgatg aactcctgaa tctcctccac
     6541 ggtcatctcc cgtgaaggaa ctttatctga ccagcggttc ggagacgggc tgggtgcggc
     6601 tgtgatcttg tcgagatcca taaaaggttt cgccagtacc cgcaatgcct tattggtgta
     6661 cagggtctcc atcatttcac tgatggtaaa tgatctgccg aaacctgccg tcagctggac
     6721 gaacagcttt gctcctgtct tgtggaactc cggcatccac tttgccagat cctcatacat
     6781 ttttttcttc ttatatagcc actgtccgta catgggattg tataccggct gacatcccgg
     6841 aagcaccaga ccgcaattat ttttcgctac ttccagaata aatttcgcac catccttgtc
     6901 aaaatggttc acttccatcc atcccaacag gtttgtgcca cccatggatg tcagaacaat
     6961 gcggttctta atctcgcagt ttccgatctt ccacggcgta agcaacggtt ccaactgttg
     7021 tttcatcgta actcctcctt tgccttctct ctt
//
LOCUS       DAWDHD010000129         7015 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_628_length_7015_cov_8.690805, whole genome shotgun sequence.
ACCESSION   DAWDHD010000129 DAWDHD010000000
VERSION     DAWDHD010000129.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 7015)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7015)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7015
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_628_length_7015_cov_8.690805"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1618 a         2028 c         1481 g         1888 t
ORIGIN      
        1 cgtcagatct gcggcattga ccaccacatc cacggcatcc ccattgatgc tgaccacagc
       61 tgcactgaaa cctttcgcct gcaaaagcac ctcggccgcc gcttcctttt ccgcgatatc
      121 agtcatgcgc accatgctgt ccaccgcttc ctgtttctgg gcatcgctga gctcagtact
      181 gttgataatc tccaataagg tctctttatt ccgggctctg gtctgctcct tggaaagtct
      241 tgcagcagac agtacctcca caccgttcgt tccggtcaac actgcttccc cgggaatctc
      301 cccttcgtcc ggcgtagcct ctgccacctg catatcttca tccagataat cttccatgac
      361 gatctccaca tccgaatcca ggctttccat atctgtgaca atgtctgtgg aatccactgc
      421 actgagatct tctgtattat ccgacgccag atactccttt tccaggttgg tccccgcaaa
      481 ctgcagataa cctgcaatgg ctatcatgat ggcaagtgcg gtaatcataa gctgattctt
      541 cttaattaag tttttcactg cgttcccctt ttccaaaggc ctgtatctgc cttcttgatt
      601 ttatactcta ttgtatgtac ccgtctgtgc ggatatgttt cctgtgactc atttttccaa
      661 caccttgact ttgtgggctt ccacaccgaa aagtgcctgt gcgatctgcg tgatttcact
      721 ggtcatattg ccttttccgg ctccttccgc caccaccagc accccctcca ctttgggtgt
      781 gatcgtcttc gctacaaacg gtgtgccctg actgccgtcc tgatagacgg tctggtatcc
      841 ggtattggaa gaactcacgg tgcgggttcc tccggcggag tcctgttctt ctgtctggga
      901 acgatccgcg gtcatgtctt tttccacaat tctttcctcc gaggattcca atgtgatcat
      961 gacttccacc tctcccagtc cccggacgga ttccagcatt ttcttcagtt ttccctccag
     1021 ataggcggta tattcctccg cgcttcccgc tgccaccgcc cgggtttcgg tatctaccgc
     1081 cgtctcctgt tccccggttg tctgcgaagg agaagtgaaa attccggcat tgtactgttc
     1141 tgcagtctct gtgccgacgg aagctgcctg tggctcctcc tttttcaccg gcagggcgat
     1201 gataaacaat aggacaccca acaataccag aatcaacagg ttgtcccgac ggaaccattt
     1261 cggagggccg gaaccgcctg taccgttacc tgtaccaatg ctgcctgtga tcttttccca
     1321 gaattcaccc attgcttctc acctcttttt cttccctggc tccggttttt ctaccgtcat
     1381 tttcctcagc tcttcggcag acttctccat cattccagcc ggattggtgc aatgtccaca
     1441 tggattccct ccgtttctgc attttcccta tccgtttcct tctgatccga tacttcccca
     1501 ctgttttctc ctgtaacagc tccctgtgca aaatcttgct cccacacatt tgccgggtca
     1561 tatccttccc cggcttcctg cgccacagca gctgccgtat tcccgatctc ttcctgcgac
     1621 tgtcctgcct gttcggaggc ccgggtcagc acagtctgca attcttgcgt aaatcctgtc
     1681 acctgttctt ctgcattcca gccgtctccg ccgccgaaca tggtcagaac aggctgtagc
     1741 aactgcagca acagcataac ggataataac agtttcaaat atttatcgta ggactccttc
     1801 ggtctgaaat gcactacggt ctgtgcacag atcagaaaga tcccggcctg tccgatcacc
     1861 cggtataacc attgcatcct cttacctccc cctgcttccg atagctaata gtgccaatgt
     1921 gatcagaaac aacagcatgg ctgtgcccgc agtacgaaac agaagcatcc ctgcatttcc
     1981 catatgatcc gtacagttgg tcactctttt atcgctgacg atccccataa aagctgccgc
     2041 acacttgatc aaaaacgcag taaacaaaat ctccagaagc ggagccgcac acgccgccag
     2101 aagcagtaac agcaaaagta cgccgatgct gttgcggatg acaacggctg accccaccgc
     2161 cagctccaga agcccgtctg ccgcatttcc gatccccggc agtgccgaaa gtgccttttc
     2221 cacggcggag gtcttcacgg aatccaatac cggtgtgatc aatgactgga acacgctgat
     2281 ccccgtgacc actcccagag aagccttgag aatccagtcc accgcctttt ccagaagttc
     2341 cgtcaaaagt gtcagctttt cctccaccca gatcccgttg atcatggtca acaggccata
     2401 gcagtaaatc agtggtagta ccacacccaa cagtattttt tccgtaccat agatcagaag
     2461 cagcatcagc tgatacccgg ctcccactgt agccggcccc gaggccactc ccaccgcaag
     2521 aagatacgta ggtaccatca gccttacaaa caacacgata ttttccaccg tctctcttgc
     2581 ggttcccaga atcccggaaa aacattgcag caggatcact cccatgagca gataaataaa
     2641 gtaaaagctt aaatccgcaa tctgatgttt gtcgaaaacc tccacgaaat gaatcagcag
     2701 tgcagaaagg atccccagta cgatcagcca gatcagaata tttttcctgc ccgcagcaga
     2761 tgctcccatt ccctgtatca cttcctgcaa cagggttccc gctgccccca gcacatctcc
     2821 ctgcagcagc tgtcccatca attccgccac ggagatccgt gaggccggaa acagcttctg
     2881 catccctttt tccagttctt ccatgccata ttcctgccac attgcatcca ttgctccctc
     2941 actcttataa gataccatag atctgtcgga tcaccgtaag cagaatcggc agtcccgcaa
     3001 acagtaccga aagttttccc agaatctcga tttgatccga taccgctgca aatccggcat
     3061 ccttgcagat tccggcgctg aattcacaca ggtaggtgat ccccaccact ttcatcaaaa
     3121 taccgagata cccttttgca tccccgaggt actgctgtaa tcccgcaaac gcctccagca
     3181 actgtcccgt cagccccagc acataggaaa ataccagcac actcagggca aatccgatca
     3241 gcagcgcata ttccggccgc tgttgcctaa attgtatggc aaaaagaact ccggcgattc
     3301 caagcaatgc aatcttaatg atctcggaca tatttcctcc tatcaaaacg aaaacagagt
     3361 ctgtacagta tcaaacagat catagatata cggaacgatc cagctaagaa ccaggatcag
     3421 tcccgccagg ctgacgagaa aagcatgttc ctccctgccg ctgtgtttta agacctggct
     3481 gagtatcgtt acaaggattc ctaccgcagc tattttaaag atcagtccta ttcccatctt
     3541 tccccgtcct tttctcttct ctctgtgatc cccggaaccg gcatcagact aacaataaca
     3601 gtatcagcag accgctcatg gctcccagtc ctacggagag cctgcatttc tgtgtaattt
     3661 cccgctgctg catatccgcc gtatgttcca gtccctgtct ggacaattcg atacttttta
     3721 tctgcatcgt ttcatccgca tagccctctt tttccgcaaa acgcaaaaac tcttcaatgt
     3781 cttctttttt cagaggaagc atcctcagct gtctgcgaaa gcttttctcc gccagttcca
     3841 gaaaaccagc atcctccgtc tccgcatatt cacggtatac ttcggtgcag atctctccca
     3901 gcctgtcttt ccgttgtctg ccagtctgcc ggcagcattc cggaagtgtt gacccgttgt
     3961 accggatctc actgatccac tgttccagaa tgcggatgag ttcccggatc gtatataatc
     4021 ttcctaagaa ttgctgccga taccacagtc ccattccaaa gcatcccaac agaatcagaa
     4081 atcctccgaa catctgcatt ccatccgtcc ccttcctttt aagatcacta tgcggtcaaa
     4141 tatctgtcgc aggcaggatt cacccaggcg ctttgctgcg tccgtctcgt ccgttccatg
     4201 cagagtggcc agcagaccgc agccgcaggc cgctgctttt tgtaaggccc gcctgtccgt
     4261 ttcctccccc agttcatcca ctgcaatgac ttgaggtgac atggaacgca aaagtaataa
     4321 catcccctgg gccttgggac agccatcaag cacatccgtc cgcatcccca gatcattctg
     4381 aggtcttccc agatagcttc ccgccagctc cgagcgctcg tccaccactc ccacccgaag
     4441 tcccgcacca tacgtattac catcggaaat ctggcggatc agatccctga gcagagtggt
     4501 tttgccgaat cccggaggcg atacaatcag ggtattctga aattttcctt tcttatataa
     4561 ttttggcagg ataccgtcag ctgctccccg gatctcatgg gcgacccgga tattgataaa
     4621 cgcaatattt ttcagagtgc ggatcactgt ttcattttcc aacacagcct gtccgcacaa
     4681 tccgatccgg tgtccgcctt ccacagtcaa aaagccctgt ctgatctcat cggcataagc
     4741 atacaccgaa tcctgacaca gccaggcgat caactcccgt atctccgctt ccgtcaccag
     4801 atgcgctccg taaggatgct cctgccattc cccgccgtta tttaagaaat aatctcctct
     4861 cgatgtttcc acgatcaccg gcctgtccac acgtagacgg atctcgcaga ttttttcccc
     4921 attatccgct gtcttctgcc agaatgctct tctcttttgc ggaaaaatac gaagcaggct
     4981 gtccatgttt ctcctcccgt aactattcag aacctcatgc gcaaaatcgt tccttcggaa
     5041 cttctccttt gcgaatgcag gcattcatca agacttagct gatatttaat gctctgaata
     5101 gttacaatat atgtctccgt ggacaggata ttccttccaa tcttctcttt ttttctatag
     5161 tgtggtataa taagcgataa ctgtttttat gacttttgtt ttggaggttt ttatgaacat
     5221 aatcattgtg ggctgcggca aggttggcta caccctggta gagcaattag gcggtgagaa
     5281 tcacaatatc accgtgatcg ataccaatga ggaaaaggtg aattccatca cggatgatct
     5341 ggatgccatg ggcatcgtgg gcaatggtgt cagcttccag acactgtctg aggccggcat
     5401 tgaccatgcg gatctgctga tcgccgtgac cggatccgat gagcagaatc ttctgtgctg
     5461 tgtcattgcg aaaaaggcgg gacattgtaa gaccatcgcc cgtgtccgca atcctatcta
     5521 caataatgag attgcttttc tgcgggaaga actggggctt gccatgatca tcaacccgga
     5581 gctgtccagt gccgccgaga ttgcccggat cttccagttt ccttctgcag ttaagatcga
     5641 tactttttcc aagggaagga tcgagcttct gcatttccgc gtgacccatg actgtctctt
     5701 aaataattat gaactgatcc acatccgtac caccttaaaa tgcgatgtac tggtatgtat
     5761 ggtcaccaga gataatcagg tgctgattcc ccggggtgat tttgtcttcc gggaaggcga
     5821 tgtggtagct attgtgtcca ctcctcccaa ggctaatgat ttcttcaaaa agatcggtat
     5881 cggcgccggc agggcacgta ctgccatgat cgtcggtggc ggaaccattg cttattatct
     5941 ggcaagaaga cttttagaag tcggcatcca taccaagatc atcgatcagg atcccgcccg
     6001 ctgcgagcat ttgagtgaac tgctgcccaa ggcttccatc atctgcggtg acggtacgga
     6061 tgaacgtctt ctgatacagg aggggctgga gaacgtggat gcttttgctg cactgaccgg
     6121 actggatgag gaaaatatcc tcctgtcact gtatgcgaaa cggacttccc gcgcaaaggt
     6181 agtcaccaag atcaaccgta tcaacttcag tggtgtctta agtgatatca agctggatac
     6241 gatctcctat ccgcgcctgg taactgcaga tatgatcatt aaatatgcac gatccatgaa
     6301 tgaatctctc aacagtaatg tggagaatct gtataagctg gaagacggtc atgcagaggc
     6361 actggaattc tacatcaaag aggattctgc cgttaccaat atacctctga accgtctgca
     6421 catccggaaa aatatcctgg tctgttccat cgtccgcgga ggacaggcta ttattcccag
     6481 tggtcaggac gagcttcatg taggcgatta tgtggtcgta gtactgacac atgcacggct
     6541 gaatgatatt aaagatatta ttgaagggta attttgtaga aggacactat catgaatttt
     6601 gcgatattga gttttattat cggatttatt ttacaatttg aagcattatt tttactgctt
     6661 ccctggatcg ttggtatgat ctacggagaa tatcatgtgg cactgatcta tctggtaacc
     6721 gcagctgttt gtttcatttt aggaaaattg ttaagcttca aacacacagg acgttttaag
     6781 gaattgtatg tcagggaagg cttcactgcc gttgcactgg gctggtttgt catgtctgtt
     6841 ttcggtgcca ttcccttcgt tctcacccgt gagattcctt tttatattga cgctctcttt
     6901 gagaccatct ccggcttcac cactaccggt tccagtattc tgtcagatgt ggaggcatta
     6961 agctatgcca gcctgttctg gcgcagcttc acccactgga tcggcggtat gggtg
//
LOCUS       DAWDHD010000130         7003 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_629_length_7003_cov_10.083621, whole genome shotgun sequence.
ACCESSION   DAWDHD010000130 DAWDHD010000000
VERSION     DAWDHD010000130.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 7003)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7003)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7003
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_629_length_7003_cov_10.083621"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1866..1965
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1810 a         1536 c         1762 g         1795 t
ORIGIN      
        1 ctgccaatct tatttcatat cgcggcgttc gccgcatctc ggcataagtg aagacggcga
       61 atcatgcgcg agcgcccatc gctgtcttca ctatgcctat gcgcggctca ttgctcgcgt
      121 aactttccag ttaccgtaaa aagccgtctc acatttttgt gaagcggctt ttttagtgcg
      181 cccggcggag caggttgcta atcggttatt ttttactatt tagtctgcat tcagctgtcc
      241 gaggatctcg tctaccatat cctgggtagt cttaacataa ttgtccatag cggcatctac
      301 atctcctccc tttacaacaa cctcggcgat gactacattc ttagcatcat tgatcgtacc
      361 gccgtagttg gtaataccgt cacaggaagc agagtgggga gcgtctacgc tgtgttcgga
      421 gaagaactcg ttacattcct tggtcagatc ggtagcggat tcaaaaccat tctctaagga
      481 gcagatcatg gaagaaggat cgatagcatt ctttttccat aatgcactgg gatcggaagg
      541 attcagacgc agatggaact caccgtcttt gtattcatag gtcttggcat tgtcagtacc
      601 ttcaccggta acaatagtct cagcctcagt agaccaatgg tagccttctg caccgtatac
      661 ccacagggtc tgtacagtac cgccgtcgaa catggtctcg aagactgcat cgaagattgc
      721 ctgttctctg gaatcatcgc cgtcaccgtc atcaatgatg cagtatacag gagattctct
      781 gttcaggtag ccatccattt cagtgatggg ggtcagtctt gccagtccgg aatctacacc
      841 gttcttatcc atgttgttaa ccaggttatc gttccagtaa ccggaccagt aggtgaaagc
      901 accgaaggaa ccggactgat cggaagacca ccatttttca cggacatcct tggtacccat
      961 ggtcagggat tcgggatcga tgacaccgtc tgcgtaagcc tgctgtaaac gaagcagagc
     1021 atctttggtc tcctgggtgt tgaaaccgtc ataccataca ccattttcat cataggtgaa
     1081 gctggggtat gcgttctgcc agaactgagg cagatagttg gtgtaaggag cctcggaacc
     1141 gataaagcct gctgcagcta cgccgtaggt atcgttcttg ccattgccgt caggatcctg
     1201 ctcggtgaat gcctttaaca ttgcatagta ggaatcccag tcggtaatat cctcagcctt
     1261 catgccgacg gcatccagcc atgcctgctt tacataagtg atacagccgc cgccaagacc
     1321 ggtggacaga ccatactgtc tgccgtcgat acggacattc tcgtttactg cgggcagtac
     1381 catgtggctg tggaactttg cattatcata agcagctgtc atatcccaca gaagacctgt
     1441 ttttgcatat tctgcatact gaccggcatt cagcagaatg acatcggggt aatcaccgct
     1501 ggcaaacaga cggccaacgc catctacata gctggaatgg tctaactgct ggatattcag
     1561 actgatggga tggcccagtg cttcactgac agctgtgtcc cattgttcga cgaatttgtc
     1621 ctgataagca tccacagatg cattaaaggt accgtctacg acgagagtga ccttgtccag
     1681 tacataaccg tcatcagaag aagcggtact ctcggcagca gaagaagcgg tgcttccgct
     1741 atcggtggtg gatgctgcgg gggtgccgga accctggctt ccgcaggcgg tgagtgcagt
     1801 cagcatggaa actgacagca acaggcttaa gatcttgttt cttttcattg taatcctcct
     1861 tgtttnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntccga aggaaaatcc
     1981 gagcccctat ggcgaggaga ggatttatcc tccttgtttt ccgaaggaaa atccgagccc
     2041 ctatggcgag gagaggattt atcctccttg ttttaaaaat acatctctct atagcaaatg
     2101 gtttaccacc ataatgctca ttataaagtg accgcgaata cctggcctct gttgcctgta
     2161 tagatcttct gatcttcttc tgtgaagtcc ggctgggaaa atcccagata aagtgtagca
     2221 gaagtgccat caaatactct gttgccggaa ttgtctaccg tggtaaaggc catgggcgga
     2281 atggtgatcc ggaattcttt gctttctccg gcatccagac ggatccgttc gaacgcagcc
     2341 agcttgtggt tgggaacttc gttggaagaa ccgtttacat gggcatatac ctgtaaaaca
     2401 tcacttaagt cacgatccgt gggattctgg caatgtattg tgatttgtgc ggaaggaatc
     2461 tccttcgtgt aatcaacttc agttactttt gcttctgtga tctgtaattc tccgtaggtg
     2521 agtccgaaac caaaaggctt ccagggtctg ccttcaaaat aacggtaagt gcgtccggtc
     2581 atatgataat cggtaatgtc tggcagcggt ccgtcattat aatagaaggt aaggggcagc
     2641 ttgccgccgg gagatacttt gccgaacagg atgtctgcaa tggcccgtcc gccttctccg
     2701 ccaggatacc aggcatgcag tattgcaggc acctgatctt cgtaagcgga cagatcgatg
     2761 gggcttccgg ccagatcaat cagaatcacc ggagtaccgg tagcgatgac ctgttctacc
     2821 agatccctct ggatcttggg aagcagcaga tctggcttgt ctccactggc aaatgcatta
     2881 cccacatctc cctgttctcc ttccagtgtg gagtcgagac cgacacatac aatggcgagg
     2941 tcacaggatt ctgccaatgc cacagcttcg gggatacggt agttctggaa tacctttcgt
     3001 acggaagggt catcatagag cagacatccc tctgcataat tgacatccca gccggtatcc
     3061 tcgcagattc ttgtaattcc ttccactaca gtgatggaac ggggcggggt accgttgtag
     3121 ttaccaacca gtgcggcatg actgtcggca ttggggccga tgacagccat acgtttgtga
     3181 tctttcaagg acagaggcag tgtaccgtca tttttcaaca gaacacagct ttccacagcg
     3241 gcctgataag ccagttcccg atgttcttta cagcccacca cattgtaggg aatttcgtcc
     3301 agggaactgt gatcgaagag tcctaacagg tatctggtgg tgaaaagacg ttctgcactg
     3361 cggcggattt cttcttcggt tacctttccc tgtttatatg cctgtaaaat atattcatag
     3421 gtacagccgc agtttaaatc acaacccttt tccagggcaa gagcggcgga atcctccgga
     3481 aatgcagtta catgatgtcc ctcgtggaaa tcacggatcg cccagcagtc ggaaacgtaa
     3541 tgtccttcaa aatgccattt gtcgcgaagc acctcctcca taagatagga gtgagcacag
     3601 cagggttcct cgttggtacg gttataagca cccatgacag cttccacatc agcctgtgtc
     3661 accagagctt caaaagcggg aagataagtt tcccacagat ccttcttcgt agctttggcg
     3721 tcgaaactgt gacgcagagc ctcgggaccg gaatgcaccg caaaatgttt ggcacaggcg
     3781 gcagtggtca ggtaaccgtc tcttttggtc tgtaatcctt ccacaaaggc acagccaagg
     3841 gaagcggtaa gtacgggatc ttctccgtag gtctcctggc ctcttcccca gcggggatca
     3901 cggaagatat tgatattggg agaccacatg gtcacacctt tgtaccgggt gcggtctccg
     3961 aggtgggaat aagcgttata ttttgcccgg gcttccaaag cgatggaacc ggcgattttc
     4021 tgtaataatt cacggtcaaa agttgcaccc aggccaatcg cctgcggata acaggtggca
     4081 gtaccggctc tggcaacacc atgaagggct tcactccacc agtcataggc gggaatgccc
     4141 agacggggaa tcgcgggaga cgagtgtaac agctgggaag cagcttcttc tattgtcatt
     4201 tgtgttacca gtgcggtagc tttttctctg gcttctgtac gattcataga ctatctcgtt
     4261 tctttgtttc aaggttatag gttttaagat tggaacctct ttcatttaaa ctaattgtac
     4321 gtgaaaaata tgtggactgc acgacaaaaa gaaattacat atgagacaaa aattgctatt
     4381 tatagaaaaa acagtggaaa tctgagacgg aatatgatat gtagaataca gagagcacaa
     4441 atttgattcg agattcctcc gacggaagga atcgtctgcg gaattggaga gattggtatg
     4501 aacacagtaa aaccgtttta tgagagccgg aaaaaggtct atgactgtca tgtctggtgg
     4561 cacccgaact tcccgtttca tctgcataaa catatggaat tcgtccatgt gacggaaggg
     4621 gagatggatg ccacggtagc tggtgtgaat tatcatctga ttacaggaga ctgtctgatg
     4681 atctacccga accagatcca ttcctacagt tctgtgggag aggtgggaat gcttctgatc
     4741 attgcagata tggattcgat tggagaattt cagaaggaat taacctatta tgatatggaa
     4801 tctccccatt ttcgtaagaa cgcgctcagc aggtatggtc agatggtgtt agacattctg
     4861 tcggatattg gtctgaataa ggagttacga ccgttcaggc aggacaaggg acttctgatg
     4921 gcgctgctgg ctgatatgta tgaaaatgtt ccggtcatta agaaggacaa gccttcagac
     4981 ctgaatatca cgcagaaact tctgcaatat attaacgaca atattaccgg agaactgacg
     5041 gcacagaagg tggcaaaaga gctgggaatc agtccgtatt atctgtccca cattttttct
     5101 tctcagttga atatttcatt tccatcttat gtggcgcagc aacggatgat catggcctgt
     5161 aatctgctga aggacagcag gaaaacggtg accgaaatca gttatgaatg cggattccct
     5221 aatatgcgga cattccatcg ctgttttcag aaaaggtatg aatgcactcc tacccagtgg
     5281 cgggagtgga gtcacaaggg aatggatgag aaactaaggc atcagctttg aataaaaggt
     5341 ggtgaatgaa aatctgaaag tgggcagacg gaagccggaa tttcgagaaa aaataaatag
     5401 aaaatgaaag atagaaaact gagagagacg aaaagaaagg ataactatta tgaaaaaaca
     5461 ggtctataat ccttatttgc caagctatga atatattcca gatggggaac cgagggtgtt
     5521 cggtgaaaga ctctatatct atggaagtca tgatcgcttc ggaggtaatt attattgtga
     5581 aaatgattat gtctgctggt cggctccatt gtctgatcta tcggattgga aatacgaggg
     5641 tgtaattttc cggcacacag atcatataaa ggatgaatgc cataagaagg cggaaaagaa
     5701 tggacgctat tacttgtttg caccggatgt gatccaggga agaaacggca gattctacat
     5761 gtattattct atcgcggatt cctcgatcat ctctgtggca gtctgtgata ccccggcggg
     5821 gcattatact tatttgggag atgtagtcta tccggacggt cagattctcg gggaagcagc
     5881 aggagaatat tttcagtttg atcccagtat ttttattgat gacgatggac gaatctatct
     5941 ttattctggg ttctgcccca caaaccgtga agtggatagc tttggaagaa aatttgtggg
     6001 atgccacatg tgtgaactgg aacaggacat gctgacggta cggagaggcc caattttgac
     6061 catttccaga gaatcctccg ccgcagcggg ggcagtctat ttcgaagcac cgtccatgcg
     6121 aaaaataaat ggacgctatt atcttgtcta ctccgcaaga aatacgggat tgtattattt
     6181 tatcagtgac agaccagaca aagattttcg gttcggcggc cggatccatt ccacttccga
     6241 tgtgggaatg aatggctatt ccgaggagga accttgctat cccatgggaa atacccatgg
     6301 aggactggtg gagcttaacg gcagatacta tatttttgac cataggcata caaacaatag
     6361 ttctttctgc agacagggtg tagcggaaca gataaacatt gatgcacagg ggctgattgc
     6421 ccaggtggag tctaccagct gtgggctgaa caacggatgg cttataggag aggggactta
     6481 tccggcgtac attgcctgtg tattatatta tgaaaagaca gtaccaatag atgatagaac
     6541 cgcatgcatc acacaggatg gggaggacag agagagcctg ccgggacagt acattcgggg
     6601 aataagcgac ggttgtattg tgggataccg ttattttcaa ggaaaggagt tatcaaatat
     6661 ccgtctgaca ttgcggggga ggatgaaagg aaccttgtat gtcacctgcc atgaggaagg
     6721 aggaaggatg caggatcccg aaaaaattgc agtaggaagc tgcatctgcg acattgctgc
     6781 tggggactgg gaggcgatta cgattccggt acaagtcagg gccggagtgt atcctttgta
     6841 tttccggttc cgtggggaag gcagtttaga tctggctgaa tttacactgg agtaaaagat
     6901 ttccttttct ctgctttgga agtgtagtat actgtctgtg gataacttta tgacaaggat
     6961 aatgttagcg aaaggcggta aatcaatgac agcggagatc atc
//
LOCUS       DAWDHD010000131         6921 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_639_length_6921_cov_11.540926, whole genome shotgun sequence.
ACCESSION   DAWDHD010000131 DAWDHD010000000
VERSION     DAWDHD010000131.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 6921)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6921)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6921
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_639_length_6921_cov_11.540926"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1916 a         1472 c         1965 g         1568 t
ORIGIN      
        1 tgcaaaatgc tggagagcgt ggtatccaga tacaaaaaag gacagatgaa tcccagcgtc
       61 acgatgaaat gcccggccag ttcactgtgg aacaattcgg ttcccatcca atcccccaga
      121 aaaagaaaac cgcacagaca aagaaagccc atgagactgc agtattttac ggtacgcaga
      181 gtgtcgttcc atacggcttc ccggtcacct tttgcatgat tttcggagat ggtggggagg
      241 agaagaaccg ccacagaact ggtgagagca ttgggaaaaa agatcatggg catggccata
      301 ccggtgagta cgccgtatac acttaaggct gtggtggtat cataaccgta gagcagaagc
      361 ttctggggaa tggagacagc ttcgatgctc tgcaaaatat tcagaacgat acgattggcg
      421 gtcaggggca gtgccatgcc caacagatcc cggtagaccg cggaagaagg acagatattt
      481 tgcagggaaa gaacagaggc atccggatac aggtgtatgt acaggcagat cagagcgatc
      541 agcatggaca gaagctcgcc gacggtcaga cctaagacgg ccgcattgat ggagggggtg
      601 cgcccctcag acagatcggc tccggtgacg agaaagacac agccgactct ggccagctgc
      661 tccagaagct gcgtgagggc aggaacggaa gctttctttt ttccatagaa atagccgttg
      721 atgcaggcat gcagcgcggc aaaaggaata gagaaggaca gaatccgcag catggatatg
      781 gtacgggctt cttttaacag gaaggttcct atgggttctg cggcgagaaa caggacagca
      841 ttgactccaa gggacagagg gagagtgatg cttgccccgg tcagctgcgt tttcaggtca
      901 tcggaacgcc gccccgcagt attggcggca acgaatttgg agatggcggt ctggtagccg
      961 gcagctgtca gggaaaagga gagagacagt gcgggactga gcagctgata gatccccatt
     1021 ccttcttctc cgaagagcct ggacaggtag atccggtaga aaaaaccgat gatgcgggtg
     1081 ataagaccgg tgatcgtcag aatcagagta ccgaagatca gaggatgacg tttggcatgc
     1141 ttcatagctt ttcgttggat cctttgcaga taaaagatct tgcaaaagta tattccatcc
     1201 accttgttga cagaactgcg cggtgaatgc tatcatgaat aacagtgaaa taagcatgaa
     1261 aatatgcgga aatgaggcaa tatgatatat ttagataatg cagcaactac caaaacggca
     1321 ccggaggtcg tagatgcgat gcttccgtac ttcagtgaat actacggcaa tgccagcacc
     1381 atctacagtc tgggtgccga gagcaagaag gccatggatc atgcccgcca gaccatcgca
     1441 gacagtctgg gtgcaaagcc ggaagagatc tattttactg ccggcggttc cgagtccgat
     1501 aactgggcgc tgaaagctac cgcagaagcc tatgcttcca agggaaaaca tatcattacc
     1561 acgaagatcg agcatcatgc cattctccat acctgtgagt atctggagaa gagaggcttt
     1621 gagatcactt atctgaatgt ggacagagac ggactgatca gtctggacga gctgaaggca
     1681 gccattcgcc ctgacacgat tctgatcagt gtaatgtttg caaataatga gatcggtacc
     1741 attgaaccca ttgccgagat cggtgagatc gcgaaggagc acggtgtatt gtttcatacc
     1801 gatgcggtac aggcgtatgc acaggttccc atcaatgtgg atgaaatgca catcgacatg
     1861 ctcagtgcca gcggccacaa attaaacggc cccaagggta tcggcttctt atatatccgc
     1921 aagggtgtga agatccgttc ctttgtacat ggcggagcac aggagagaag ccgcagagcc
     1981 ggtacagaga acatgcctgg tatcgtggga ctgggtgctg ctgtagagcg cgctatgcgt
     2041 attatggata ccaagacccg caaggagatc gaactgcggg attatctgat cggacgtctg
     2101 gagaacgaga tccctcactg ctggctgaac ggtcaccgca cgaagagact tccgaacaat
     2161 atcaacttct ccttcctgtt catcgaagga gaatccatgt tgatcatgct ggatatgaag
     2221 ggcatttgcg cttccagcgg ttccgcatgt acttccggat ctctggatcc ttcccatgta
     2281 ctgctggcta tcggcttaaa gcatgaggaa gcacacggct ccttgcgtct gaccttatct
     2341 gaggaaagca cgaaggaaga gatggacatc gtggcggaag aagtgaagaa aatcgtacag
     2401 agactgcggg atatgtctcc tttatatgaa gatttcctga aaagccagaa gaacaactaa
     2461 gtgagaaaga agaggataac agatacatgt acagtgaaaa agtaatggac cactttcaga
     2521 accccagaaa tgtaggggag atcgaggatg ccagcggcgt aggtaccgtg ggcaatgcaa
     2581 agtgcggaga tattatgaga atgttcctgg acattgacga gaacgggatc atccgggaag
     2641 caaagttcaa gaccttcggc tgcggcgctg ccgtagctac cagctccatg gctaccgaac
     2701 tggtaaaggg taagaccatt caggaagcat tacaggtaac gaacaaggct gtaatggagg
     2761 ctctggacgg actgccgccc gtaaaggtac actgctcact gcttgcagag gaagcgattc
     2821 atgcagcact gtgggattat gcagagaaga accatatcac catcgaagga ctgcagaagc
     2881 ccaagaatga catcagtgag ggcgaagaag aggaagatta ttaagatcta tgaagaaaaa
     2941 agtagtagta ggcatgtccg ggggagtaga ttcctccgtg gcagccatgt tattgaagga
     3001 acagggctat gacgtgatcg gcgtcactat gcagatctgg caggacgaag aggaagtagc
     3061 caaggaagcc aacggtggct gttgcggact gtcggcggtg gatgatgcca gaagagtagc
     3121 acagagactg gagattcctt attatgtcat gaactttaag aaggaattca aatgccatgt
     3181 catggactat tttgtggcag agtatttaag aggccatacc cccaatccct gtattgcctg
     3241 caaccgttat gtgaagtggg aatccctgct gcagagaagc ctggagatcg gggcggatta
     3301 tattgccacg ggacattatg cgaggattga caggctgccg aatggccgtt ttgccatccg
     3361 taattccgtt accgcagcca aggatcagac ctatgcgttg tacaatctga cccaggagca
     3421 gctggcacat accctgatgc cggtgggtga atataccaag gatgaaatcc gcgtcctggc
     3481 agaggaggcg ggacttccgg tggcgcataa gccggacagt caggagatct gtttcgtgcc
     3541 ggacaatgat tatgcttcct ttattgacag agaagccggc gaaaaggtgc cgggaccggg
     3601 caattttgtc acggaggacg gcaggatctt aggccggcat aagggcatta cccattatac
     3661 actgggacag cgcagaggac tggaacttcc tatgggggaa agagtgtttg tcaccgccat
     3721 ccgtcccgaa accaatgaag tcgtcatcgg ttccaatcag gaactcttta cggataaagt
     3781 gctgtgcgat catgtgaatt acatgacagt ggaagatctg acagagccca ccagagtatt
     3841 ggcgaagatc cgttacaacc ataagggaga atacggaact ctgacaaaac agccggacgg
     3901 taaagtgctc tgtacctttg acgcaccggt acgtgccgca acaccggggc aggccatggt
     3961 attttatcag gatgagcatg tactgggcgg cggaaccatt attctgtagg aaagcgctca
     4021 aagaggaggt ctctgtgtcc tacggataca aagatttgta tagcggaaaa gatcgggatc
     4081 actattgtta caatagtggt cctgcatttt tatcacggcg tatgacaaca gtggcaccga
     4141 cgtgcatata atagtagaaa gcttttaaca acagcaggag tgtttatgga ttatcgtttt
     4201 tcatttcaga gacctatgag aagaagcagt ttcggctcca tgaagggaac cattgtggat
     4261 ctggtaccgg caggacaggg tggccgcaga gccgatggat gccttttgtt cgctacaatc
     4321 gaggatacgg acggcaacac ggtgaacttt ttcatcaacc cggatacgta tgtggtggat
     4381 ttcactacac tgtcggttgg aatgatggca accttctggt atcgcaacga tgcgcccatg
     4441 cccctgatct atccgcctca gtatacggca gtggtagtgg ctgaagagag aagcgaccgg
     4501 aatgtggatg tgagctacta cagcaattcc ctggtcaatg aggagcagac attgcagctg
     4561 aatctggatg gcacagtgga tgtgcgtacc gtgaacaacc agtatttcca ggcgagtccc
     4621 gcgaaccata atctggtggt catctattcc tcttccacga gaagcattcc tgcccagacc
     4681 acgccgtcta cagtggtggt actgtgtgac agaaactgtg gttaggcaga caaaagagga
     4741 cagttgtagt ccattgacct attgagattt ttgcaaaaat gttataccat aaaataatcc
     4801 cctatagaat gatgtcgggg gcaaaagtcc ccgactttga tgcctacgga ttgttccgtg
     4861 ctgcgcactc ccacaatccg ccccaatatt cgcatatcgt gggattatca tcccactttt
     4921 atgctcatat cggggcgggt cccaaggtct ttcgcccaca tatgagcaag ctcatgtggg
     4981 cttagacctt ttgaccctgg caccatagaa tatacccatg gctgtctgtg cgcagtgcag
     5041 gcagcctgta agggggagat tacttatgat ctggcagaac gaaagagaaa agaggacatt
     5101 atgggtaaga aaaagcagat gcgttctgaa gtaaaaggga atgtgaaaaa cagtatagga
     5161 agaattgcgt ttgcagcatt ggctgtgctc ttacagatcg gctggatatt gatattgatg
     5221 atcaaactgt atcagtattc ttcagtgatc tctctgctga ccagcctgtt tgcgctggta
     5281 gtggcgctca ggatttatgg gaaacatacc aatgccgcat ttaagatgcc ctggattatt
     5341 gtaatattgg catttcctgt attcggtctg tgtatttacg gattgttcgg gcataaagag
     5401 gctatgcata aaatcatccg gaaatttgag gatgtggatg cacaactgat tcctctgaat
     5461 gtgcaggatg agaccatatt gcagcagctg gagcaggaag atcctctgct ggcaaatcaa
     5521 tgccattata tctggaagta tggtaattat ccggtgtatg acacgacagc tgtgaaattc
     5581 tatccggaag cttatctggg atacgaggaa cagcttaagg agctggagaa ggcaaagcac
     5641 tttatattcc tggaatatca tgccatagag gaggcggagg cttttgcacg gctgagggat
     5701 gtattggcaa gaaaggctgc tgagggcgtg gaagtccgta tcctgtacga cgatgtgggc
     5761 tgtatcggct ttctggataa aagctttata gaccgcatga atgtcattgg tgtacagtgc
     5821 cgggtattta actatgtagt gcctttttta aatattttta tgaataaccg cgatcatcgt
     5881 aagatcatgg tcatcgacgg aaaagtggga ttcaccggtg gatataacct ggcggacgag
     5941 tatttcaata tcacgcatcc ttacggttac tggaaggata ccggagtgaa gctgaccggc
     6001 agagcggtac agaactttac catgatgttc ttggaaatgt ggaatgttat gggacaggcg
     6061 gacacggatt atgaaaagta tctgaaggcg tcgcaggaag gtgtggaaga cggaaatgtg
     6121 cagaaggcgt ccggctatgt acagccctat gcggattctc ctctggacgg agagccggtg
     6181 ggagagaatg tctacctgaa cctgatcaag acagcgaaaa aacgcctcta tgtggcaact
     6241 ccttatctga tcatcagtga tgagatgacg agagagctgg gactggcggc aaaacgtggc
     6301 gtggatgtac gggtatttac ccccggtatt ccggataaaa aagtaatcta cggtgtgacc
     6361 agatcctatt attccggact ggtgagccag ggtgtgcggg tttatgaata taccccgggt
     6421 ttcctgcatg cgaagcagat gctctgcgat gaggatacgg cgaccgtcgg taccatcaat
     6481 atggattacc ggagcctgta tcatcatttt gaaaacggtg tgtggatgca cggctgtgat
     6541 gccatccggg atattgaggc ggattttgat aagctgttac aggatagtga ggaagtcacc
     6601 gataaatacc gggatggaag gaaaaatatg gcagtgagag gctggcagtg tatcatgcgc
     6661 ctgatcgcgc cgttgctgta acagacataa taacggaaag ctggagatta cgatgaaaat
     6721 actggtcagt gcctgtctgc tgggcgaaaa ctgtaaatat aacgggaaaa ataattacag
     6781 cgagcgtgtg gcaaaatatc tggaagggca tgaggtgatc ccggtatgtc ctgaggtgct
     6841 gggtggactt ccgaccccga ggattcggtc ggagatcgtg agcggtgagg tcatatcctg
     6901 taaaggcgta aatgtggaca g
//
LOCUS       DAWDHD010000132         6882 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_641_length_6882_cov_10.114106, whole genome shotgun sequence.
ACCESSION   DAWDHD010000132 DAWDHD010000000
VERSION     DAWDHD010000132.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 6882)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6882)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6882
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_641_length_6882_cov_10.114106"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1758 a         1380 c         1922 g         1822 t
ORIGIN      
        1 gccatggcca tcaccaccgc attgttcaat ctggaagcgg tagagatcac aaaatattat
       61 atgaaccggg agatccggaa agtaagtcat tatgatgact taactcaggg aagacaggct
      121 atgacggaca ttcttgtgac cctgcttgtc ttttcgctat ttttgctttc taacctgtat
      181 tctcccaaga atacagcttt tttcggcttt gattatgcgt atcgctgtat ggggatttat
      241 acgccgaatc ctttctggaa tgccacttat ctggccacca gaccttttgc gattatctgc
      301 ttttttgaga cggtgaaagt gctgtcggaa tatcagaagg attttcagtg gaagaactgc
      361 gttctcttcg cggtcagcct tctgctgacc accatgacca aaccaagcta tacgatggta
      421 gtggtgcctc tgatcggtct gatcctcctg atccagctga tcgtaagcag aggaaaaagt
      481 tttcgcaatg ccttctgtct gtgtgtgacc atgatcccca cgggaattgc cctgctgtac
      541 cagttttccg gaattttcac cgggacgaat gccatgggag aggagacagg catcgccatc
      601 ggctttgcca aagtatggag caattattcc aaaagcattc ctttaagtat tatcatggga
      661 atggcattgc ccatcggcgt acttttcctg aatctggtat ttgattttaa aaatataaag
      721 tccaacagat attactggtt tgcatggctg aattatctta tgggtacagt aatgttcctg
      781 atcttctatg aaaaaggatt ccgcatgatg catgccaact tttcctgggg ctatatgcac
      841 ggcatgttct ttgtcttcct gatgacgttg atcgtcatgg tgcggaatat cagggagtgg
      901 tggaagagct ggaaggtgat cttcgtcgtg ggcgagatcg cggtattctg ctaccatctg
      961 gtgtgtggtg tgaatttcct gatgtatgcc gtcctgggca acgatctggc gggattttag
     1021 taaaaatgat ttgaaaaggc agacaagata ccatcttgct gcgagttaca tagaataata
     1081 aaagaaagga gagtgtttca tgaatttaag cattgtagcc ggtcttctga ttccgtttct
     1141 gggaacgact gcaggggcag cctgcgtgtt tttcctgaaa aaacagattg gcggcaatat
     1201 tcagagaata tttaccggat tcgctgccgg cgtcatggtg gctgcttcgg ggtggtcttt
     1261 actgattcgt gccagggaga tgtgtgtaga gatggggaag cttgcatttc ttcccgcagt
     1321 gatcggattc ctgatcggta ttgttttttt gttgttcatc gatagcatgg taccccatct
     1381 gcatgtggga agcgatgcac cggaaggcca taagagcagt ctgaaccgta cggctatgct
     1441 gatgttagct gtgaccattc ataatttccc cgagggtgcg gcgtgtggtg ctatttttgc
     1501 cggcgtgctg agtggtgacg gaacggttac gatggcggga gccctgacgc tggccatcgg
     1561 tatcgccatt cagaatttcc cggagggagc cattatttcc ctgcctcttc ggagtgaagg
     1621 aaataagaga ggaaagtcct ttgctttagg cgtattgtcg ggagcagtgg aaccggtggg
     1681 agcaatcctg gccattgcac tggcaagcat tgtgacacct attttacctt atatgctggc
     1741 ttttgcagcc ggagccatga tctatgtggt cgtggaggaa ctgattccgg aagccagtga
     1801 gggagagcat tcgaatctgg gaaccattgc ctttgcgatt ggttttgcat tgatgatgat
     1861 gttggatgtg gcactgggat aatgtgtaca gggtaacatt tgcaaaagca aagtgagaac
     1921 agtaggaagg atgtacggga atgtccgtgg aaaatgaaaa agaaatacaa cagctgaaaa
     1981 accggatccg tgaactggca gacaaatcct acaaccagaa ccagtacaca tttaccggat
     2041 tcttaggact ggcggagcag gatgccctgt ggcaggtgga acgggaagtg aagtttgcag
     2101 ggatcacctt atacggcggc agggaagagg cagagcggaa gctccttcgc ttcggcagtg
     2161 aggcggagct gggctatgaa cagccttttc ccatctgctg tatccggatc cgtcctctgt
     2221 cggcaaaatt tgcagacaag ctgtcccaca gggattatct gggggcactg atgaacctgg
     2281 gtattgaacg gagcaccatc ggtgatatcc tgccgggaga gggcgaagca tttttattct
     2341 gtctggatac catcgcggaa ttcgtctgtg acaatctgga gcagatccgc cacacccacg
     2401 tgaagtgtga ggtggtggat gcggcggcag aagtaccgcg ggaagaaccg ttaacacagg
     2461 tcattcaggt ggcaagtcca cgtgttgatg ctgtcatttc caaggtatat aataaatcca
     2521 gaagtgactg cctggagctg ttccgggcgg gaaaggtata tgtgaacggc aggctgtgtg
     2581 aaaataattc caagcccctg gagacggggg atgtggtaaa tgccagaggc tacggtaaat
     2641 tccgcataag tggagaaccc catgccacga ggaaggggaa gcttgccata gaggctgagg
     2701 tatatccgta gcggttcggt catgagatga cagttgcctt gctcgtaagg aaaaaacgaa
     2761 gacgtgaagg aggttaaaca gtgtatcatt atactgcgat ccaatggcta tttttctttt
     2821 atttttattg ctttttcggc tggtgttttg aatccaccta tgtatccctg aaaagcagga
     2881 agctggtcaa ccgtggtttc tgccgcggac cgtttctgcc gctgtatgga agtggagcca
     2941 tcatgatgct ggtggtgtcc atgccgtttc aggacaatct ggtgttagtc tatattgccg
     3001 gatgtatcgg ggctacggtg ctggagtatg tcacaggcgt gactatggag gcgttattta
     3061 aagtcagata ttgggactat tccaagaata aattcaattt ccagggacat atctgcctgg
     3121 gggcctcgct ggcatggggt ggtctgacga tcctgatgac ggagtttatc cataagccca
     3181 ttgaacatct ggtattgtcc attccggaca gcatcctcac tcctgtgaca ctggtgctga
     3241 cggcgctgat cggtgcggat ttcgcattat ccttcaaggc agccctggat ctgcgggatg
     3301 tactggagaa gatggagaag gcaaaagaag atatgctgcg gatccagaaa cggctggatg
     3361 tcattattgc tgtgaccagt gaagatctgg agaaccgcag ggaaggcttc agtgatagcc
     3421 tggcacagaa gaaggaagac tttactgcaa gtattaccac cagagtggag gacctgaagt
     3481 ccaacattga gggcaagctg gagagcttcc gcacttccgc acagaaatct cccaatcagt
     3541 atttggagag cgtgaagaac gaggtcgccg agttgcggca gaagtacacc agaagcgtgg
     3601 cagacagaga gaatgtcagc agcttaagag atttcttcca gcgtgacatg attcgttcca
     3661 accctaccat gagctctgag aaattcaagg aagctctgga agaactgaaa gagcactcca
     3721 ataagaaaaa tgaataaaaa gcagaaaaaa gcatgtctgt gatgcccgtt tttagggggg
     3781 cggaagggca tgcttttttt gtacatggaa aatgagaaaa aaatgaaaaa aaaggaagaa
     3841 atagggttga caatataacg ggggggggta aactaaataa cggtaaaaat aaaatccata
     3901 acattatact taggaggaat gagataatga aaaagaaatt aatcattgcc gcagccattg
     3961 catttgcagt atttgcagtt ccttacggca gcagtgcact gtctaccgta tctgtagcaa
     4021 gtgagaataa tacggtggca ccgacacctg cacctgttgt agatgtagat aaggtgaagg
     4081 ttagcattga taacattagc attacagatg tgtccagcgc agacgaggca gtagctattt
     4141 tgaagaataa cattggaatc caaactttga gggccgcgtt gtggcagagt tatgatctta
     4201 gagaggaatt gtatggccgt ctggaacagg aatatgcaga tgcaaaagga atccagattt
     4261 ctaaacctaa ctttacagac gattttaata aagaatattc agatgagtgg tgggtatcca
     4321 gtgaaggagt agctcttaat gattgcactg agttacaggt ggatgctcct acaatcaaac
     4381 cttccgtttc tggtgtcaca tctatatgtt atttggactt cagcctgatt aatgcagaag
     4441 gacggaaagt tggtgctaca gtgaacgttc cggtaggtat cggagtatct gttcctgcgg
     4501 gaatggaccc cgcgaaagta gtagtttatc atttcgcagc agatggcagc atagatgaga
     4561 ttattgatcc ggaatggtat gtagatgaga ccagcggcag agattctgta tattttctag
     4621 tagatcactt ctccactttt gccatcgcag aagttgagac taccggcgct gaaggtactg
     4681 acaacgcatt tggaagatat cgtgacaatg tggcaagcga gatcgctaat gccaaggacg
     4741 gtgctaccgt taagatcagc agagacaaga atatcaatgc tctgcccaac gatatcatgc
     4801 aggctctgta caagaagcag acggtagctc tggagctgga gtacaccttc gaaggcaacg
     4861 agtatacggt aaccatccct gccggtaagg cagaagacaa tgctatcgaa tggtatggtc
     4921 ctctgtacct gcagatgcgt tacggcaaat aagtaaccgt aaggaaatga aataaccggg
     4981 aaatataagg cactcttcag tcaatgaggg gtgccttgtt tttcagtctg aaggataatg
     5041 taagctatga agaaacagga acaaaataca aaagagaccg tgagtatgct ggtttatggg
     5101 tatctggtga ttctttttgc cggtttaccg ctgtatatgc agaacaagct ggtgatgatc
     5161 ggaaatgcaa aatatttgtt tttccggaat actacgctgg tgctgggggc ttttgtggtg
     5221 ctggctgtgc tgtggcagag gatccgggga gaacggacaa cgaaacggac gtggaagaaa
     5281 acggatgtgt ttatgctgtt gtatctggtg tctgcaatct tttcttatgg aatcagtccc
     5341 tgcagggagg atgtcctttt agggtatccc ggctggtata tggggctggt gacccaggga
     5401 ctgctggtag gaatctattt tgcggtatcc cggtattatg acggcagcag aagtatctgg
     5461 tggattgccg ggatcacagc gggaatcgtg gcactgatcg gactgttgaa ccgtctggac
     5521 atcgatgtgc tgggaacttt ccgcggaatg gaaaacggag aatggaaccg gacccagctg
     5581 ttgtccacca tcggtaacaa caactggtat gcaggctata tcagtgtgac ggcaggtatc
     5641 tctctggcag cagcttttat gggaaaacaa caggtcagag cactgggact gctcgggagc
     5701 tttttgtttt ttgcatcggc tattacttcc aacagtacta cagctatact ggcagcctgc
     5761 ggattaagcc tgctgttact gttgctttct ctgcggaaga gaggccggct gctgcgggcg
     5821 ttggagatcc tgatgctttt accgctttcg gtatttatgg tcaggatgtt tttgctgtta
     5881 catctgaccg gcctggtgct ggcaggggat gccgaaaaaa gactcttttt taccccggcc
     5941 tggtatgtgg tgtttgttgt ggaagtggcg gtatatctga ttttgcagct gcgggagaga
     6001 caggagagaa gtgaccggct ggagagtggc cgggtttttc ggaccgttgt ggggcttgcg
     6061 gttacggtga cgctggcggc actgcttctt ggctgtctgc tggtggcggg atatctgccc
     6121 ggcagtgata aagtgtcgga agctgccaac ggccgactgg cactatggaa ggtcacgatt
     6181 cttacttacg gaaaagaggg actcctgttt cagatattcg gtatgggtcc tgacagcttc
     6241 tattatgcgc tctatcaatg gggcagtgac gccatggact ggatcaaccg gggacttctg
     6301 gacaacaata tctattccaa tgcccacaac gaatggctga ccctcctggt acagcaggga
     6361 attctgggag tcattgccta cggtggaatc ttcctcaccg cattccggaa ccttcgaatc
     6421 tccgcaacca gggatccccg ggcacttgca gttttcctcg gcctcaccgg ctatttgatc
     6481 tgctcactgt tcacttttca acatgtgttg tccacaccct ttgcctttgc tcttctggga
     6541 atggcagagg gtgtttttat gtaaagtcat cttaataaag ttttaaaggt ttttcctgtt
     6601 ttttattaat ctggattctg ttacaatgat tttagcgtaa gggaagccct tgcggaaaag
     6661 gtgccgggcg gcaatggccg gtatggaaat gagggagagt atgtacaata tattgatctg
     6721 tgatgatgag aaggatatcg taaacgcgtt aaagatatac ttaaatgaca ccaattatca
     6781 ttttctggag gcttttgacg gtgaggaagc cgtacatatg gtagagacga atgaggtgca
     6841 tctggtactg atggatatta tgatgccgaa gctggacggc at
//
LOCUS       DAWDHD010000133         6865 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_642_length_6865_cov_9.903818, whole genome shotgun sequence.
ACCESSION   DAWDHD010000133 DAWDHD010000000
VERSION     DAWDHD010000133.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 6865)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6865)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6865
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_642_length_6865_cov_9.903818"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1653 a         1849 c         1493 g         1870 t
ORIGIN      
        1 cgtagtgcgg atcggcagtc gcaaacatcg tgttagctcc accctgttct tcaacacttt
       61 tcaagaaaaa gtcaacactc tacgacaaac atcatcgcca tggcatccat ccacttcgtc
      121 agttcatgac tttacccggt aattacatcg ctgcatcgat cacatcagac atgttgtaca
      181 tgccggcggg cttgcctgcc aggaacttgg ctgcctgtac ggcgccttta ccgaagacag
      241 ctttggagta ggcggtgtgg gagaaggtta ccacttcgtc tgccccggcg aagatcacat
      301 catggtcacc gacgatggtg ccgcctctga cagagctgat gccaatttct ttcttcggtc
      361 tctgctctct gcggttgctg cggtcataga catattcata ctcaccgtta aattcctcgt
      421 tgatggaatc tgccagtgcc agtgcggtgc cgctgggagc gtctaccttc agattgtggt
      481 gcttctccac aatctccata tcgaagcctg cgttggccag cactccggca gcttctttca
      541 aaagctttaa catcaggttg atacctaagg acatattggc ggattttaag atggccacct
      601 ttttcgctgc ttcgtctact ttcgccagct gtgcctcgga cagaccagtg gtacacagca
      661 cgcagggaag ctttttctcc acgcagtaat ccaacagctt gtctactgcc acggctgcgg
      721 agaaatcaat gattgcatct gcagccacat cacatttgta aatatctgtg aatacagggt
      781 agggattgtg tccatcgtct ctggcatcaa ttcccgccac cagttccata tcggggtccg
      841 ctgcgatcag tccgctgatc acctgtccca ttttaccgtt acatccatgc atgattattt
      901 ttaccatttt tcttttctcc tatctatcat gatttttgta ataacgatcc agcagccctc
      961 atattcattt gcaaaagcgc actatgaatc gttacttttt gaaaaaagat cccgcccatg
     1021 ataaaaattt cacaggcggg attcttccac tcttgctctc tattttccag cctacaggac
     1081 accataatcc ttcagggctt tttcaagcct cttggcattg gcctcttcca tttcacacag
     1141 aggcatacgg aacgcaccgg ctcccattcc catcatgttc agtgcggtct tcacagggat
     1201 cgggttcact tcacagaaca gtgcgttaca cagatccatg gcatctaact gcatctgacg
     1261 gctgcctgcc acatctccgt caaagaactt ctggcagatc tcgtgggtct gtgtaggtgc
     1321 tacattggaa agtacggaga taactccgac tccgcccagg gccagaatgg gtacgatctg
     1381 gtcgtcatta ccggaataaa tatccacaac gccctgtccg atggctgcca ggtgagcgat
     1441 ctggctgatg ttgccgctgg cttccttgac acctacgatg ttctccacgt ccttacacag
     1501 cttcactacg gtctccggca gaatattgca gccggtacgg ctgggtacgt tatacagaat
     1561 agcgggaacc ttcacagaat ccgcaacggt cttgaaatga cgatacagtc ccttctgggt
     1621 tgctttatta tagtatggac ttaccagcag aatgccgtcc acgccgtatt tctccgcttc
     1681 ctgagacaga tatactgcag tctcggtaca attggaaccg gtacctgcca ctacggggat
     1741 tctctttgca actttgtcca cacagtattt gatagtatcc agatgctcct catgggtcag
     1801 ggtagatgac tcacctgtgg taccgcatac gataatagca tctgtgccgt ttgcgatctg
     1861 gaattcgatc agttcaccga atttctcata attgacagat ccgtcctcgt tcatgggtgt
     1921 gacaattgcc acgcctgctc ctttaaaaat agccatatgc ttcctccatt tgcaaaacgc
     1981 cagcgcacaa tggcgccggc gctgattatc caagcttatt tgatagcgcc ccatctgctc
     2041 acgagatgac agtcatacgc ttcttaaacg tatgcccaag ataacatata caggatacct
     2101 tatctttcgg cgtgttcccc tttcttcttc cctcatcggt acctgttacc tccgaaaaaa
     2161 tactcttgaa aaacgcaacc tctatcttct taaatataat tttatgatag catcaaccca
     2221 tgtgactgtc aatcccagac cttgaatttt accgtttcaa tcttggacac ttcatccgcc
     2281 agaacacaga tctcatcgtt cagggtgatt cccgtcagaa tcacatccgt atctccccgg
     2341 cattccagta atgccttcag gtcatcttcc gtaataatat ccttctccac cagtcccagc
     2401 acttcgtcca ggatcagcag atcgcattct ccagtcgtca gtactttctt ggcaaagccg
     2461 atcccgttac ggatgttctg gatctcctcc tgcttctttt cctccggtaa ttccacgaaa
     2521 ttaccgtccg atttctcgaa acggaacagc ttgatctccg gctccattct gcgtaaaaaa
     2581 tcggaatccc ccagtccctt tcccttcagg aactgaataa taacgaccct cttgccttct
     2641 accgctgcct gtaccgctct tccaatcgct gccggtgatt tgcctctgcc atcgccggta
     2701 taaatataga tgcatccttc tgccattcca cacctcatgg tctatctgcg tatctgacgc
     2761 tccctagctg tacacagtta ctaatatcta tgcattcttt tataccatat attgtcactt
     2821 tttgcaatct tttgtcgtta gacgatggat tcctgcacct gtgcctcctc cagaagttcc
     2881 agtttgctga cggatacctc gaaagctact cttttctcca catgctcttc atccagtttt
     2941 ttcatatatt cacggctctg gatccgtccc cagatcgcac agcgctctcc taccttgaaa
     3001 tgctccgcat aacgggcgtt acgtccccaa cagatacagg gaatataatc ggattttccg
     3061 tacggacggt ttaccgccag cagcagatcc gctatttctc ttcccaacgg tgtcttacga
     3121 tacaccggtt ccttacatat atatccatcc agataaatct ggttcgtctt cgaactttct
     3181 tccatttctt caataaattt cacttctctg gcaaacacag acaacaccag tctgttcttg
     3241 cgctcctcat gacgattgta ggaacgaaac tgtccgttca cacacaaaag cattccgaca
     3301 tagctggctg tcacgtccag taatctttct gagaccatca ccggtataat gtcaaaactg
     3361 tcgctcagtc tcgtacattt gatgtccacc atgtagaatc cttccccata gatctcatgg
     3421 ctgtaggaaa aattgcttac cacttccccc atcaccgtta cctgattgtt ttcaataacc
     3481 ttatctgtca tttttgttct cccttctaca tcttctgact cttctacaac aatctttatg
     3541 tattgcacta ctatttatac tctaaaatcc atcgtttgag tactaaaact catcgcacaa
     3601 aagggcaaat tacgcacaaa agggatttct tttccatact ttttcatata gaaaaccacc
     3661 aaaagcgttg attttccacg gttttttctt gcgtccggaa atgataagtg ctatactgaa
     3721 acagtttgaa aaaagattga atcagttgca cgcattttta gaaaggcaag gatttttatg
     3781 agacaaaaaa gagaagatgt cagaaacgtt gcaatcattg ctcacgtaga ccacggtaaa
     3841 accactctgg tggatgagct gttaaaacaa agcggtgtat tccgtgaaaa tcaggaagtt
     3901 gctgagcgtg tcatggactc caacgatatc gagcgtgagc gtggtatcac cattctgtcc
     3961 aagaacactg ccgttatgta taagggtacc aagatcaaca tcatcgacac cccgggccat
     4021 gcggacttcg gcggcgaggt agagcgtgtc ctgaaaatgg taaacggtgt tatcctggtg
     4081 gtggatgcct tcgagggtgc catgccccag accaaattcg tgcttcgtaa agcactggaa
     4141 ttaaagcttc ccgttatcgt atgtatcaat aagatcgacc gtcccgaagc aagacccgat
     4201 gaggtcgtag acgaggtact ggaactgttc ttagagctgg atgcggatga ttctcagctg
     4261 gattgtcctt tcgtatatgc ttccgcaaag gccggtattg cctctctgga tgctgacgaa
     4321 atgggtactg atatggaacc tctgtttgag accattttaa actacatccc cgctcccgaa
     4381 ggagatccgg atgcggctac ccaggtactg atcagtacca ttgattataa tgaatatgta
     4441 ggacgtatcg gtgtcggtaa agtagacaac ggtaccatcc gtgtaaacca ggatgtggtc
     4501 atctgtaacc accacaaccc cgacaagatc attaagacca aggtcagcaa gctgtatgag
     4561 ttcgacggtc tgaataaagt agaagtcaag gaagcaggca tcggttccat cgttgccatc
     4621 tctggtatca gcgagcttca catcggtgac accatctgct ctccggaaaa tgtggtaccc
     4681 attcctttcc agaagatcag tgagcctacc atcgccatgc agttcatggt caacgactct
     4741 cccctggcag gccaggaagg taaattcgtt accagccgtc acctgcgtga cagactgttc
     4801 cgcgagttaa ataccgatgt atccttgcgt gtggaagaga ccgattctcc tgaccgtttc
     4861 aaagtatccg gccgtggtga gctgcatctg tccgtgttga tcgagaatat gcgtcgtgaa
     4921 ggttttgagt ttgcagtaag taaggctgag gttctgtatc ataccgatga gaacggcaag
     4981 aagttagaac ctatggagct ggcttacatc gatgttccca atgaatttgc cggtgctgtt
     5041 attgagaagc ttggtcagag aaaaggtgaa cttcgtaaca tgggttccat ttccggcggc
     5101 acttacaccc gtctggagtt ctccattcct gcaagaggac tgattggtta ccgcggtgag
     5161 ttcatgaccg ataccaaggg taacggtatt ctgaacaccg tattcgacga ctacggccct
     5221 tacaagggag atatccagta tcgtaagatg ggttccctga tcgcatttga agccggtgaa
     5281 gctatcacct acggtctgta caatgcgcag gagcgtggta ctctcttcat cggacccggt
     5341 gaaaaggtat actctggtat ggtcatcggt cagaccggac gcagtgagga catcgagatc
     5401 aatgtctgca agaagaagca gctgaccaac acccgttcct ccagcgctga cgaagctctg
     5461 cgtctgacac cacccaaaat cctgtctctg gagcaggctc tggacttcat cgataccgac
     5521 gaactgttgg aagtaactcc taccaacctg cgtatccgta agaagattct ggatccgacc
     5581 atgaggaaac gtgccatgat caataagaaa gcttaagaag atttataaat agaaccacct
     5641 tcgtgtactg cctgcagtat gtgaaggtgg tttttaactg ggttaacaaa gaatcccaaa
     5701 tgcccacggc gaacggaaca tccttcctcg atgtaaggtc tcccggtggg aattttctga
     5761 cgaaaagtga gtagcggttc taaaccgcac gggcacggct ccagttgcca tccatggcaa
     5821 cgtccgcctg tcgcgaacgt cctgttcgct ccaccctgat gttcaacact tttcgagaaa
     5881 atatcaccac ctcccgacaa acatcttcgg aaggacattc catccgccgc gggcattctg
     5941 cgttttggaa agcctgcgaa aaaacagtag cccgcgttgg ggtaaggaca agcagactct
     6001 aagcagccgt cggtacttag cgagggtact ttcctcgctt atgtaccgct acggtctgcg
     6061 tccgaagtgg aaacgggtct gcggttctta gccccaatac gggctacggg gatatttcac
     6121 taactcttca gttaattcca ggtggctgtc aggacatgct ccccggtcat ctggacgacg
     6181 gtgtctgcgg taataatctg gccgctgtat tcccatccgc cgaaggcact gccctctttt
     6241 accggggtag gaagttcacc gtaggtctcg ccgaaggtca cctgtttgga ttccacggtg
     6301 gaaccgtcgg caccggtgcc gccgtcatag tcgaatttca cggtgaactg tgcgggaacc
     6361 cagtgagcgg tcagagtcat atcgccggtg actttctccg actggaaata ccacagcact
     6421 gtctctccct ctttttccgt ataccacccg tcaaaggtgt agccgggctt ataaggggtc
     6481 tcgggctctg taaggaattc accgtagcgt accttccggg ctgccacttc acttccgccg
     6541 tccgtatcgt acacaatgtt gaatccaccg cgtaccgcga agaaaataat cattcccacg
     6601 atcaccacga tcaggacacc gatcaggccg tccagaatgc ggatgggaac atccttgctt
     6661 ccgtaaatgc cgcggccgaa ccattttcct ttcggacgga ttgcttcctc cggctgctgc
     6721 gtcgtttccg actgcgtggt gacttctgct gtcttttctt ctgtattctc tgttgtcact
     6781 tgttcctgat tcataatttt tccttttcct aatctatcat aagcaatttc tatgtgctgc
     6841 gcactctcga aattgcaacc aatat
//
LOCUS       DAWDHD010000134         6861 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_643_length_6861_cov_8.325448, whole genome shotgun sequence.
ACCESSION   DAWDHD010000134 DAWDHD010000000
VERSION     DAWDHD010000134.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 6861)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6861)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6861
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_643_length_6861_cov_8.325448"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1673 a         1836 c         1555 g         1797 t
ORIGIN      
        1 ttgcaagaaa aaaagtataa actctttata aactccgtca atcctccagt acccggttca
       61 gaacttcatc ttccagcacc agtttctttg ctgcttcgcg gtatttttcc tcgtggagat
      121 aggtatggcg gtggggtttg atggtgggag ccatgtcgat ggctttgagg aaatcggcct
      181 tcttcatatg taaggttgcc acgtaatccc agaatccggt atctttaagt accgtgctga
      241 cacggatgta acggtgatcc tgtacctttg ccatgatata ggtggcaata cctacctgga
      301 tgccatgaag ctgcggcact tccaggatct tatccaatgc atgggaaatc aggtgctcac
      361 ttccgctggt aggggcactg ctgccggcaa tctcgttggc aataccgctc atggccaaag
      421 aatccagcag ttcttttagg aacagctcgt ccttgatgct ctcataaggg gtacgaacga
      481 agctgttcac cgctttcttt gcgatcatca ctgcaaaatc attcacctca ccgcagccgg
      541 ccttttcttc gaaaatccag tcatagagtg ccgtgatctt ggaaatcata tctccgatgc
      601 cggagtaaat gaacttctcc ggagcggtac ggatcacctg ggtatccacg ataatgccat
      661 aagccagtct ggcagggaca gaagttcgct ttccctgcac cagaagggat gcgctggcac
      721 tggaaaagcc gtcgcttgaa ctggaagtcg gcacactgat aaaaggaagg ttccgcagaa
      781 atgccgcata ttttccggca tcgatgacct tgcctccacc gatagagatc accgcctttg
      841 ccttgttggg aatagcgaaa gccagcgtaa tgatatcgtc aatatccacc gtatccaatt
      901 cattgtattc cagaacattc acgtccgctt ccttcagtga attcatgacc agctctccga
      961 acatatcgat cagtccgtta ccgaaaaata acactacctg atccagacct tccgttttta
     1021 aataactacc gatcttacct aatgccccgt ttccgatctt caggatggac gggatcgcca
     1081 tctcttttcc tatctgcatg gtaatctctc ctttaacatt tcataaaaaa actttgcccg
     1141 aaaccgcgtc cggacaaagt cattttacac tgttttctat ttcagttcaa cttacaattg
     1201 gatcccgaac atctgcagca cccagcagat cagtcccaat gcccagctgg cgaaaattcc
     1261 gtacaggatc acaggtcctg caatggtgaa tatcttgcag ccgataccga atacctgtcc
     1321 ttctttctgg tattccaccg ccggggctgt cacggaattg gcaaagccgg tgatgggtac
     1381 taaggctcct gcacctcccc atttgacgat cttgggataa atatgcagtg ccgtgagtac
     1441 caccgatgca aacaccagga tcagggaaca ccagctgcct gccgtctggg gatccattcc
     1501 cagcacattg ccggtatagt tcaggattcc ctgcccgata cagcagatga tcccaccggt
     1561 cagaaacgct tttcccatct gtgcccacag attgtgggtg ggggtcaccg atttgacata
     1621 ctgttcgtat tcctgcttct gctgattgcc ctgttttgtg tcttcctgtt tcatagatgt
     1681 accatgcctt tccgccgccc ggccgcttgc acggcaggca gactctgtga ctgctctctc
     1741 tgataacaga atgtcctttt tcactctttt tatgtatgct tctatttaca aattttcttt
     1801 taacacagtt ttcttccata aaaacagact tctcaacagc tccgcaacct ctgccacggc
     1861 ggtatccata tcatttccgg tatccgtgat ctgttccgca tccataagat tatgatcttc
     1921 tgccagtgtc ttgtgcacca tatccggtgt cgccaccatc tgctgcaatt tcaccgcgtc
     1981 ataataatac cccggtgtca catccaccgg aagccatcca ataccgtcga aatagacctc
     2041 cgcccagcag gatcctgcgg ccgcggaacg cgttatccgc attctgttcc cgattctctg
     2101 ttgtcgtttt ctcttcgata ctgccttcct ctgccagagg cagctgcaaa aacacggtga
     2161 aatccgagcc cttatctttc agactctcta cgacgatttc tccgcccatg agctgtacca
     2221 gctgatccgt gatggccata ccgattccgg tgtcatgtac ccggatcatg aggtccacac
     2281 cgttttcctg taaaaaagtc ttttcttctt tcctgtggtt ctgactgtcc accggattcc
     2341 ggctccccat ctttttccga aaaaaagaaa gaaccagaat tttgtttttt ctttggtatt
     2401 ttttcagatt gtaagcaatt acactgtttg acgccctctt tttttcatgc gataatgggc
     2461 gatgagtata aattttattt catgagggag attattatga aaaaaagaac aaggaaaaag
     2521 ttacgggccc tttggggtct gctgctggtc cccgtggttc tgaccgggtg ccgcatccgt
     2581 accacgccta tgggcgtatt tgcacagatc ctggagtatg ccggacaaaa caattcgcag
     2641 gcatcctcgc ctcacggtca tggtacttac cataccgaat cacagccgtc ttccacccct
     2701 attccccaga tggattacga ctcgctggat gctatcggtg aggtccagac catcatggtc
     2761 tatctggtag gctcagatct ggaatccgat tacggtaatg ccagtctgga tctggacgag
     2821 atggaaacgg ccggagtgga cacagcgcac aataatattc tggtctatgc cggtggtgca
     2881 tccgaatggc aggaccgcgg cctgtccagt gacgaatgca ccgtcctgct gttgaccgac
     2941 accggtttcg tccccgtgga tacctatccg gcggaaaaca tgggggatcc cctgactttg
     3001 agcagctttt taaattacgg ttttgatttc ttcccggcag attcctacag cctgatcctc
     3061 tgggatcacg gcggcggacc ggtgctgggc tacggcgtag atgagaattt ccgtgacctt
     3121 ctgacgctgg atgaactgtc cgaggcgctg gaagattccg tgggcgccca catgacaaaa
     3181 ctggaatgga tcggctttga tgcctgcctg atgagttccc tggaagtggc ttccgtgctg
     3241 gctccctacg ccaactatat gattgcctcc caggagaccg aacccggttg gggctggaat
     3301 tacgatttcc tgtcagatct gtccgatgag gtgatcccgg gcgatgtcat gggggaatat
     3361 atcgtggatt cctacatgga ttacggggaa tacgtcttcg acatctatcc gaatctgtat
     3421 tccgacctga cgctctcctg cgtcgatctg agcgcctatg ctgaagccga agaagctctg
     3481 aacgattatt ttgcggaact ggacacttct ctggatgtgc agaattaccc cagactggtg
     3541 cgtaaccgtg cccgggtgag agacttcggc acttactcct ccgatatgaa ttacggcatg
     3601 gtggatgtcc tgcatctgtt agaactggtg gggaatgatt ctgaagccgc acaggctgcc
     3661 acagaggccg tagaaaactg tatcgtctat tctggcacca acatggacaa tgccggagga
     3721 atctctatct gctatcctta ccagacggat acggattacc gggatgcctg cattgaaatg
     3781 ttatattatc tggactttgc tcccaattac acccgttttc tggaagactt ttacgccatt
     3841 gaaaacggtg acaccctgct ggcagatcga gagatctcca atgcggagac ttccgtcacc
     3901 acccagaacg acggtgctta cgatgaatcc gacattaccg tgcagctgac ccccgaacag
     3961 caggcgaact ttgcctccgg cggctactac atcctctgca aagccaggga cgaaggctat
     4021 attaccgcgg aggaagacga acgtgccgat gatatgtatc tcttcattca gggaagtacc
     4081 cgtgtgactc tggatgaaaa tggtttctta catgctgctt acaaaaataa tgctgtctac
     4141 atgcaggatc aggacggtct ctccgatatt cccatgatcc taacagagac cgatatcagc
     4201 gacacagaga atcgttatct ggcacatgcg gtgctgatga attatactga caactgggaa
     4261 tcccagacgg cgaaagtaca gatcgtcgtc agcgacgaat atcccgatgg tatcatccgt
     4321 agtgcgattc ctctggatca tgacgatgcg gaattgcaga gtgccagcaa gcagttgatc
     4381 catctggatg actatgagac tatgtcctta gtggcaagat gcagctacgt gacccgggat
     4441 gataacggta atctgttgaa cttcttcgac tgggaaaaat ccggctggat gatgggcttc
     4501 gatgtggatc tgaccggcga ttatcatacc gtggtacagc cgctggatca cccggaaaat
     4561 tacgtatgca tcttcttcat gaaggattcc caggggaata cctcttattc cgaaatgatt
     4621 ccgctaaaat aagggaaggt cagctttcca gctcttttcc ttctcttcct gcttgttcga
     4681 tcagggtctt atccacgtcg gtctcgtagt ttttgatgga gacttcgacg ggggtaggat
     4741 ccttggtcag gcgcctgcca ccgacatgat tgaagaaaac gataatgcca gctgccaacc
     4801 caagagaata gaatacttcc aacgtattca agcctccggg ttcagcaccc atggccatcc
     4861 ggtacacttc cgtgaaaacc tcattgatcc ccacatcatt atggtaagcc atgatcgtat
     4921 aggctgtacc gaaaaagctg atcaggcaca caaaagcagc cttgatccac tgctgccagc
     4981 ctttgtgtct gttcacacag atccattcca ccagcacatc tgtctctcca accacctgca
     5041 catcgatctc cggacagatt tcttccataa gacttaccag tttcagacaa ctgatcacac
     5101 atcgtttttc gccgtccttg ggaaaatgat aaattttgat ggcttttagt tttgcctgca
     5161 ataccggatc ctgacagcga atctctgcca catctttcag gaaaacatcc tgtgcctgca
     5221 cctccgcact tctgtcacat ttcagataaa ccgtctgctg cttcatatac cctgtccctt
     5281 cctgccgttt ctgctctgca tagacatatt ttcccagatt gtccatatta tttgtgtgct
     5341 tgctcctccg gaagaattcc cctgcttatt gcacggtccg atgcagttct gtggcaaaat
     5401 aaaacagtga cccgcagatc tttcccgctg ccatccccag aatggccccg cccagcccgt
     5461 ggcggaaaga gattcttctg gcaaaaatag gaatactgtc cagcatctca gcaatggcca
     5521 gcgccagaca accgatgaac attccggcaa acagcccgaa aaccgcctgt gctccaatgc
     5581 ccgctgccgt ccacagcctc atctgttccg ggaagtgttc ccgcagataa tttccccact
     5641 gactgtactc tgggaaaatc gtaacaaaac atcccgccag cgtcccgaaa atgaccattt
     5701 cctcatagag aagtacataa cgtgcggtat gggtcttgcc tgcgaaccgg gggatcagcc
     5761 ccaccgccac cagcactgta aacactccgg ctgccgacaa caggccgtaa ctggccccgg
     5821 ttactatgag caataattgt ctgatcagca tctccgttcc cctttcgcaa agttctgctc
     5881 ccggcagtta tcaaaggatc tgcaaatgat ctttttctgc acacaataga atatcccaaa
     5941 accgaaaatc atatacgtat ggcacttttt tcccaaatgt gctatactat ctttatatat
     6001 gtgcagacgc tgccccactt actgtttgcg ccgttttgag tgaagaattt tagatttcaa
     6061 aatatatgtg aggttttaca actatgaata cagcagatac gactgaaaat acagaaaaaa
     6121 agacgagtgc acttcccgcg aaaaaagtct ccgattccga gacacatcag ctgcatgtgg
     6181 tcattcatcc ggatatcaac ggtttcgggc gcctgttcgg cggaagactg ttggagtgga
     6241 tcgatgaagt agccggagcc actgccagac gtcactgcgg cagggatgcc accaccgtag
     6301 ccatcgacaa cctgtatttt aaatccggtg cctatctgaa tgacattatt gtgctgatcg
     6361 gtaaagtcac ccatgtggga cacacttcca tggaagtccg cgtggacacc taccgcgaag
     6421 agcaggacgg caccagacat cccatcaacc gggcttactt cgtcatggta gccatgggcg
     6481 acgatggaaa gcccactccg gttcccggcc tcattttaga gaacgacggg gaacgcatgg
     6541 aatgggataa tgccattaag agaagagagt tacgattagt tcgtaagaag gaaggattct
     6601 aaatccgatt ctcgtcgcat ccgcagatcg gagaactcag aaatcttcga tctcccggat
     6661 aatggatcag cggttcgatc acattatggt aattttcccg atacaggtca tctctgatcc
     6721 agaagatttt tccgtaatcc tcgtgtatct gaaaagaagt aaagccttcc ggagatttta
     6781 aaagacaata tttgttttct cctctacggc attcgcattc caacagtacc ataaactttt
     6841 tcatgaatct tccttttcat t
//
LOCUS       DAWDHD010000135         6848 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_647_length_6848_cov_12.368468, whole genome shotgun sequence.
ACCESSION   DAWDHD010000135 DAWDHD010000000
VERSION     DAWDHD010000135.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 6848)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6848)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6848
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_647_length_6848_cov_12.368468"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1678 a         1876 c         1478 g         1816 t
ORIGIN      
        1 acaaaggact ggtggaagcc ggcttcttag gctacgtcaa tgaccagacg aaagtcacct
       61 tcccctggag catgatcgac aagatcaccc ccagacctga tgccaaagta gaagctatgc
      121 tggccgagga tcatatcgac ggtctggatg ccgtagttac ttccaaaaac acctatatcg
      181 ctccctttgt caatgcggaa gaatgcgaat acctggtcat tgaagatgct tttccgaacg
      241 gtaagcccgc actggacaaa ggcggcatca tcttcaccga cagagcgacc gtagacaaag
      301 tggagaaaat gaaggtatgt acctgcctga atcctctgca caccgcactg gccatctatg
      361 gctgccttct gggatatacc ctgatctccg aagagatgaa gaacccgtta ttgaaaaata
      421 tggtggaagt catcggttac aaagaaggac ttcccgtagt ggtaaatccc ggcatcctgg
      481 atccgaagaa attcattgac gaagtggtga atgtgaggat ccccaatccc ttcctgccgg
      541 attctcccca gagaatcgct accgatacct cccagaagct ttccatccgc ttcggcgaga
      601 ccatcaaagc ttacgaagct tctccagacc tgcatacgga agacctgaag ctgattcctc
      661 tggtctatgc cggatggctc cggtatctca tgggaatcga tgatgagggc agagaattta
      721 cgcccagctc cgaccccctg ttagaggaag cccgtcagta cgttgcagac tacgaactta
      781 gtttctctcc caaggatctg tcaaagctgg acgcattact tgccaatgag aaaatattcg
      841 gtgtgaacct ccacgccatc ggcatggata ctctggtaaa acagtatttt gcagaattaa
      901 gctccggtgt gggggccgtg gctgcagccc tgaaaaaata tgttcctgag aaggtaaccc
      961 tttgatctta gaagttacca cttctcataa ggacaccggt tgacggcgat tacagcccgc
     1021 agttccacaa agcagccaca ggcaccgcac aggccctcat tcagatattt gcattcctta
     1081 catacagcaa ggcggtcttc atagaccgcc ttatctgctt tactatcctc ttcgatcctg
     1141 tctatgtagg catacagatt ctccatatag gcattcttgt ccatttcccg caacagacat
     1201 ttccgacagg aatattttat tgtttctctt atttgttctt cttccataac tgttcttatc
     1261 gcttatttta tccggaacat aacgacactg catgccggca tggtaaaggt gatcctgcca
     1321 tctgcgatct tatactccgt aaatacttct tcgtgtacct tatcgggatc ctcaaaggtg
     1381 ttgtaagcat gcatatcatt ggtaagaatc gcagcggaca cgtactttgg attacactct
     1441 gcgaaagcga tctccacctc ttctgcctta tcaatggaca ggttgttcag ggtgatggtc
     1501 acaacaccgt ccttatccac ggaagcggat tcctgtaaat ggggaacctt gaacttttca
     1561 tcttcaccga tctgttccac accatcgata tagctttcca ccagatccgc atcctgatgg
     1621 tatttgtaca tatggaatac atggtaagtc ggggtacgca gcatcttcgg tccctcggtc
     1681 aggatcactg cctgcagtac gttgataagc tgtgcgatgt ttgccatctt cacacggtca
     1741 cagtgcttgt tgaaaatatt taaagacagt ccggccacca gtgcatcacg catggtgctc
     1801 tgctggtata aaaatccggg attcgtgccg ggctccacat cataccaggt gccccactca
     1861 tcgcagatca ggccgatctt cttctccgga tcgtatttgt ccatgatcgt agaatgctta
     1921 tgaatcagtt catccagctt aaaagccttt gccagcgtct tataccatgc tgcatcgtcg
     1981 aagtccagtg cacttccctt gtgagaccag tcatcctccg gcagtgtata gtaatgcagg
     2041 gaaagtccgt ccataaagcc atgcatccat tccggtgcat tttcaaaaca ggtcttcaac
     2101 acattttcag tccaataagt atctcccgca ttggggccgc aacagacttt gaaaattggg
     2161 tgcttctgat cataatttct cacataggtc tggtatctgc ggtacagatt gccatagtag
     2221 gatggggtca tattgccgcc gcatccccag ttttcgttac cgacaccgaa gtaatccacc
     2281 ttccatgctt tctctctgcc gttttttcta cgcaggtctg ccataggaga taccccgtcg
     2341 aaagtcatgt actctaccca ctcggacatc tcctgtacgg taccgcttcc cacattgcca
     2401 ttgatgtagg tcttacaacc gatctgttca cacagttcga agaactcatg ggtaccgaag
     2461 ctgttgtcct cggtcacacc accccaatga gtgttgatca ttttcttgcg gttctccttc
     2521 ggtccgatac cgtctttcca gtggtattcg tccgcgaagc acccaccggg ccagcgcagt
     2581 accggaagct tcatctcctt gagtgctgcc accacatcat tacgcatgcc tttggtattg
     2641 gggatctcgg aatcctctcc tacatacagt ccctcataaa tacatcttcc cagatgctca
     2701 gagaaattgc cgtaaagttc cggattgata tgtccctttt tgttcttttc gttaatataa
     2761 acctttgcca tattgatcct ttctgtggca ggcatccgtt tcctaccact gttgcggcat
     2821 cacgccgttc cgtcgcagtg cgattcctgc agagcgttca ttataaaata taaaagatgc
     2881 cagggtcaaa aggtctaagc ccacatgagc ttgctcatag gtgggtgaaa gaccttagga
     2941 cccgccccga tatgagcata aaagtgggat gataatccca cgatatgcga atattgggta
     3001 ggattgtggg agtgcgtagc acggaacaat ccgtaggcat caaagtcggg ggcttttgac
     3061 cccgacatca tataaaagag aattgcataa agcaatccac aactgagatt attttagcaa
     3121 agaaggtgat gcctgccaag aggaaatgaa atttataaac atttttttga aaaacgcccc
     3181 cgtgcaatca tacgatgtgg ataactttct gaattttcaa aaaagtgttc aaaaaaatat
     3241 ttttccaaaa tggcaggcac taaagtaaat ttacctttcg atgctggttc attattggaa
     3301 gcccttcact tcatgccagc tctccacagg ctccgtactt tcttccacct gtacattctc
     3361 atatactaca cttcttaact tgtcttccga aaagttatcc actcttgccc tgatccgcag
     3421 atcctgcagg gtaaaatcgg aaagcagata ctggctctca tccggcatca catcaaaaaa
     3481 ggtatcacag atgcagtcac agtccttcat cgtgatatgc tccgcataag acaacggttg
     3541 gtccttccga tccttcagat cataatactg ggtccaggga taaatcttga ggaactcctt
     3601 tacccggcct tcgatctgct ccaccgtgat atattcatag tgctgcgggg tgtccggccg
     3661 cattttcagc cacaacagtc tcgctgcccc cttgatctga atcctgcgca tgatgatatt
     3721 gcggttatgt actgattccg aaccgcaggt gagacaccca tggcagaagc catagacaca
     3781 atcctccacc agaatccgct cattggagcc gttctccggc tggctgtccg cccaggggcc
     3841 cttaccgccc ttcagcacca cagagtcgtc attgacttcc atataacagc ctttcaccaa
     3901 cacatccgta cacacatcga tatcaatggc atccgtactg ggtgccgcaa caggacctgc
     3961 cggggaataa atatggcagt tcaagtattt tacatggtca catttataaa tatgtgtggt
     4021 ccagaaatgg gaattctgta aattcagacc ggcgatccgc acatttctgc agttggacag
     4081 atagaccagc cggggtctct gaccatcctt gttggtgccg ttactccatt tccagcgcag
     4141 ccagcaggct ttccaggtct taagtccgtt accgtcaata gtgcccggtc cgcacatggt
     4201 aaatccattc agaccgtccg cattgatcag tgccgtaaaa tacaggcagg tctctccctc
     4261 aatccgggtc tcggccagat cataatccga gatatcatcg ctgccctgta acgtcgcacc
     4321 ggcggaaaca tagagattca ctccctgttt gaaataaagt gctcccgtca gataggttcc
     4381 cggcggcacc accaggacac caccgccctc ttctgccgcc aggtcgatca ccttctggat
     4441 ctctttggta taaacttttc cgtcgtccca gatcccgtac tctgtgagga catagggtct
     4501 gcccagttcc tcaagctccg gcatccctat atcataaaac cagtcatcta tgggagttcc
     4561 gtccgggaaa tattccgtag gtctttcttt ttcgttatgc attctctgcc tcctttgcgt
     4621 tctgctccct cagaaaacga acacaggatt tccgccctga tcgttatcgt tggttatctg
     4681 atttctacaa aggtagtgtc gtaaggtgca atggttaccg tacatgcgcc cgcttccgtg
     4741 gggatagtgg tggtctgctc ggtgtcactg ttgttgatga ctaccagttt tttactctcc
     4801 gggtaataag cacattccgt atacaagtta tccgtcatat agctgccgga ggtgccttct
     4861 ccaccggcat aacggatcag ctggtacagc attctggtat tccacaggtt cacctggaag
     4921 gaagacagat agattcccag tcccttgccg aaatgattca gggtgatcag tggcttcccg
     4981 ccgtctgcca gaagtacctg tgcttttcca tccgtcagat accggttctc cttcgcttcc
     5041 acacctgcgc cctctgggat cagatgctcc ggatccgctg tctcaaagat ccatctgccg
     5101 tgacagactc ttgcgccggt gtcctcgtca attcccagca catgtgccat acggaaatac
     5161 gtatcatatc cttccacagc ggaaggctcg ttcacaccga taaagcagcc tccctcgtgt
     5221 acccactttg taagtgcagt aacaagctcc tcatccttcc aggcatcccc gccgctccag
     5281 gcgcttccgg ctctacctgc attgattacg acatctacat ccttcagtat accatttttt
     5341 acatcctcaa aggaaataaa tttcacatcc acaggaagtc cggacaaagc ttcgttgatg
     5401 tggatcaggt catgcatata agtctcatga aagtgtccag acaaggtcca ggaacgtaag
     5461 cttccccaat aatgcagcac tgccacacgg gtcttgatgc ggtaaggttc tcccgcatcg
     5521 tggaagctgc ggatcagacg gaactcatct gccacttttt caatataatc acagaaatcc
     5581 gggaaaccct ccaccagatg aaggtatccg ccgagaccga tccggtcgat cttcgcacgc
     5641 agcagggcac gtcttacgct gttccagtat tttttcgcat ccagtgtggg attgccgccc
     5701 tccatgaaag tcggagcacc gcccagcccc accgggaaca gataggggtg cagacgcagt
     5761 tcatgtgtag gcacatccac gcccgcacac agtctggctt cataaccgga gaatacacat
     5821 ttgatcagtc cgtcaaaacc aaactcctga aatctgccat tataaggctc cacgcctacc
     5881 cagctgtcgt catagaagac ataagccttt ttgccgtaag aatgcaccat atcgatcagc
     5941 ttcctgccga aggaaataac gaaatcattg ataaatgcca tccaatccgc ctttttcgca
     6001 ttgccaggca tatgagtcac atgcagctgt cccttattga taaaatcttc cgctgtcatg
     6061 cgatatccgt attgtttctc gaactcctgt aaagcaggaa cgctcacagt gaaatcatag
     6121 gaaccccagt cagagaacag attccggttc ctctcactgc tgccccagat ccatacgaaa
     6181 ttgtagaaca tggaggtata gcgcaccacc gtggtatccg gatgggtctc acaccagtgc
     6241 ttcatccagc ccagcagata ctcctgagtc tcaggatatc tgggatcgat ctgcatcaga
     6301 tgctccttgt cccagtggtt cgtagtatga ttgtacatag agatttcttc ccagatacga
     6361 taggctaaga agcttactgt atacttatga aaagggatga tgccgctcag ggacacgctt
     6421 cccttctctt tgtcatagct ccagtccgca ttgtcgatct tcacattggt ggttctgtca
     6481 taaacctccc aatattccag actctctgcg ctatcattga tccggaactg ctccgcaaaa
     6541 aagctgtcca tcagtccgat ggtcagggtg tcactctctg ctgcctgggg cggtgtgcac
     6601 aggaaggtct gctgtaattt gtcctgattc tcctttgccc aggcattgtg atctctgata
     6661 atacaaatgg tagaatagat tccgtaaccg gcatgggtga tttcgtcgga aagcacggtt
     6721 ccatcactgt cacggatcac atctgctccc catttttccg ccattttcag agtcagttct
     6781 tcatatcctg attctcccgg taaggtaaaa ccaccttttc ccattctgag ttcctcctca
     6841 tattcctg
//
LOCUS       DAWDHD010000136         6818 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_650_length_6818_cov_10.458081, whole genome shotgun sequence.
ACCESSION   DAWDHD010000136 DAWDHD010000000
VERSION     DAWDHD010000136.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 6818)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6818)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6818
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_650_length_6818_cov_10.458081"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1883 a         1503 c         1854 g         1578 t
ORIGIN      
        1 gtgatgaaaa gctacacgaa agaacagttt gtaaaagatg tgatcgcagg tatcattgtg
       61 gcgatcattg ccctgccctt atccattgca ctggcactgg cttccggagt aacaccggaa
      121 aagggtatct atacagcaat caccgcaggc tttgtgatct ctttcctggg tggcagccgc
      181 gtacagatcg caggtcctac tgcggcattt gccactatcg tagccggtat tgtggcgaag
      241 aacggtatag acggactgat cattgcgaca ctgttagccg gtgtgatcct gatactgatg
      301 ggatttttac gacttggcaa tctgatcaag tttatcccct atacgatcac caccggtttt
      361 acttccggta ttgctgtgac gatctttatc ggacagatca aggacttcct gggactgacc
      421 attgtcagtg aagagccgct gatcgagacc atggacaaat taaaagcggt atttcagttt
      481 atatccacca ccaatctgca ggcccttctg gtgggtgtga tcagtctgct gatcctgatc
      541 gtatggcagt atctgccggg cttctgcaag aagattcccc cttcactgat tgcagtactg
      601 gtgggaagtg ccatggtagc actgcttaat atgaatgtaa acaccatcgg agatctgtat
      661 gagatctcca ataagctccc gacaatttct ctgccgtcct tcagcctgaa gaccgtacag
      721 aatgtcctgc cggatgcttt caccatcgct attctggcgg ctatcgagtc tctgctttcc
      781 tgtgtggtgg cagacagcat ggtgaatagt agacaccgct ccaacatgga attgatcgcc
      841 cagggtgccg gcaacattac ttccgcattg ttcggaggaa ttccggctac cggagccatt
      901 gccagaaccg cagccaacgt gaaaaacggc ggacgtaccc ccattgcagg tatggtacat
      961 tctgtgacac tgctgttaat actggtggtg ctgatgcctt atgcggcact gatccccatg
     1021 cctaccatcg cagcgatcct gttcatggtg gcatacaata tgtgtgactg gcggaagttc
     1081 gtaggactct gcaagactgc acccaagagc gatatcatcg ctctggtgac cacgtttgta
     1141 ctgaccgttg tgtttgatct ggtggtagcc attgaggtag gtatcctgct ggcagccatt
     1201 ctgttcatga aacgtatgag cgatgtgacg gaagtggaag gctggaaata tgtggacgat
     1261 gaggatgatg cggacagcct gtccttaaga gtcgtaccgc acaacactat ggtttatgag
     1321 gtcagcggcc ctctgttctt cggcgcagcg gacaagatcc tcaagatcac gctggatgag
     1381 aagatgaatt gtctggtact gcgtatgcgc agcgtgagcg ctatcgatgc cacagctatg
     1441 cataatctgg aacagctata tacagactgc aagaagaaaa atatccagat cattctgtcc
     1501 catgtagggg aacagcccat gcacgtcatg gagaagagcg gttttctgga caaggtcggc
     1561 agagagaatg tgtgtgcaca tattgatgat gcactggagc gcgccgcacg gttgggataa
     1621 ggggcaaaaa ctatcatttg tggacgggtt ctttaattta caacctcaat attttgtgct
     1681 aagatagatg cggatacgca agcttttgcg tgtccgcttt gtcgtgtatt cttttacaga
     1741 acaggagaaa atcatggctg aattgacacc aatgatgcaa aaatatatgg agaccaagga
     1801 gcagtataag gactgcatcc ttttttatcg cctgggagac ttttatgaaa tgttctttga
     1861 cgatgctata gtagcatcca aagagctgga aattgcactg accggaaaaa gctgtggact
     1921 ggaggaacgt gctcctatgt gtggcattcc ctatcatgcg gtggagacat atctggccag
     1981 actggtcagc aggggctata aagtagccat ctgcgaacag gtggaggacc caaagctggc
     2041 aaagggactg gtgaagagag aggttatccg tgtggtgacc ccggggacca acctggatgt
     2101 ccagtcgttg gaagccagca agaacaatta cctgatgtgc attgcctata cctcggatgg
     2161 aatcggcatt tccgcagcgg atgtcactac cggagattat tatgtgacag aggtagaaga
     2221 cctgcggaaa ctgaaggacg aactgatgaa atatgagccc tcggagatca tctgcaatga
     2281 ggcctttctg gtcagcggat atgatgtgga ggacttgaag tcccggttac atatgtcggt
     2341 cagctcactg gagtctcaca tgttcgatga tgacggctgc cggcggatcc ttatgagaca
     2401 ttttaaggtg aatactctga tcggcctcgg tgttgaggaa tttcctacag gaattctggc
     2461 agcaggagca ttactgcaat atttatatga tacccagaag acggatctgg aacattttac
     2521 tcatatttct ccctatctta ccagcaaata catgctgttg gacagttcca ccagaagaaa
     2581 tctggaactg acagagacac tgcgggaaaa gcagaagaga gggtctctgt tgtgggtact
     2641 ggacaagacg aagacggcca tgggcggcag attgctgcga aactatattg agcagcctct
     2701 gattgataag gaagaaatgg agaaacgtct ggatgcaatc caggaactga atcaggattc
     2761 catctcccgg gacgagatca gagagtacct gaatccggtt tacgatctgg aacgactgct
     2821 cagcaaagta acttataaga cggcaaaccc cagagatctg atcgcttttc gtaactctct
     2881 gcagatgctg ccgcctatca agacggtgtt ggcgggcttt cagaaagagg agctggcagc
     2941 catccgggaa gaaatcgatg gactggagga catttatcag ctgattgacg aggcgatcgt
     3001 tgaggaaccg cccatatcta ttcgtgaagg cggaatgatc aaggatcagt tcgatgagac
     3061 tatagatcat ctgcgtgcag ccaaacatga cggcaaacaa tggctggtcc agctggagga
     3121 agaagacaga gaacgtaccg gaatcaagaa tctcaaaatc aaaaaaaata atgtgtttgg
     3181 ctacttcttt gaggtgacca attcctacaa ggatctggtg ccggaggatt atatccgcaa
     3241 gcagaccctt gccaatgcgg agcggtatac cacaccgaga ttaaaagaac tggaagatac
     3301 gatcttaaat gcggaggaca agcttcagac actggagtat gatattttct gcagaatccg
     3361 ggataccatc gcacaggaac tggtacgtat ccagaatact gccaaggcgc ttgcaaagct
     3421 ggatgtttat gcatccctgt ccctggtgtc tgagcgcaat cactatgtac gcccgaagtt
     3481 aaatgaaaaa ggtgtcattg atatcaagga tggcagacat cctgtggtgg aacagatgat
     3541 cactaatgac atgttcattg ccaacgacac ctatctggat aatggaagtc attgtatcag
     3601 catcatcacc ggtccgaata tggcaggaaa atccacatat atgcgtcaga cggcactgat
     3661 cgtgctgatg gcacagatcg gttgttttgt gcctgccaga agtgccaaca tcggcattgt
     3721 agacagaatc tttacccgtg tgggggcatc ggatgacctg gccagcgggc agtccacgtt
     3781 catggtggag atgaatgagg tggcgaatat tttacggaat gccacatcca aaagtctcct
     3841 gatcctggat gagatcggaa gaggcacgtc cacctttgat ggcctgagca ttgcctgggc
     3901 agtgatcgaa catatcagta atcgaaagct tttgggagcg aagacactgt ttgccaccca
     3961 ttatcatgag ctgaccgagc tggaaggcaa aatgaacaat gtcaacaact actgcatcgc
     4021 agtcaaggaa tgcggagacg atattgtatt cctgcgtaag atcgtaaagg gcggtgcaga
     4081 caagagctac ggtatccagg tggccaaact ggccggtgtg ccggatatgg tcattgaccg
     4141 tgcaaaagag atcgtggaac agctgtccga caatgatatc accgaaaagg tgcagagtat
     4201 cgccattgat aataagggcg atgggaaagc aaaaaaacag ccaaaatacg atgaagtgga
     4261 tctggcgcag atgtctctgt ttgatacggt gacggacgag gatgtgctga aggaactgat
     4321 ggagatcgaa gtaacgactc tgacaccgct ggatgcactg aatactttgt accgcctgca
     4381 gaataagtta aagaaccgct ggaacggcta gaaagcataa gggaggagat ttatgggaat
     4441 acatgtactg gactcccgga cgatcgacag gatcgctgcc ggtgaggtgg tggagagacc
     4501 agccagtgtg gtaaaggaac tggtggagaa tgccattgat gccggatcta cagcaatcac
     4561 ggtggaggca aaggaaggcg gcattgaatt catccgcgtg acggataacg gctgcggtat
     4621 cgagagagat cagcttccta ccgcgttcct gcgccatgcc accagtaaaa tagaggatgc
     4681 ggatgatctg aaccatattg cgagtctggg attccgtggt gaggctttat ccagtatcgc
     4741 ggcagtttcc cgggtggaga tcattaccaa gcgaaaggaa aatctcacgg gtacccgcat
     4801 ggtactggaa ggggcgcggg agcaatccat tgatgaaatc ggtgcaccgg acggcacaac
     4861 gttcctcatg cggaacctgt tttttaatac gccggtacgc cggaagttct taaaacagcc
     4921 tgccacagag ggcagttata tcacggatct gatggaacat ctggcgttgt cgagaccgga
     4981 tatttcgttt aaattcgtac tgggaaacca gaccagattc catacctccg gaaacggtga
     5041 cctgcgggag gtaatttacc ggatctacgg cagggagatc gccgcagagc tggtgcctat
     5101 ccagatcgaa gagaacggca tcaaagtcga aggatatctg ggcaaaccgg tactggtgcg
     5161 ttccaatcgt aacttcgaga tctattttat caacggtcgt tttatccgca gcggtgtgat
     5221 cgccaaggct atcgaggaag gctacaagca gtatctgatg cagcataagt ttccactgtg
     5281 cgtactgcat atcaccatgg atacggagac ggtggatgtg aacgtacatc cttccaaaat
     5341 ggatgtgcgg ttctctgacg gcatggcatt tgccagaatg ctcagtgaga agatcgccat
     5401 cactttgcgg aaccgggaga tgatcccgga tgcttctctg gaggatgaaa aggcaatcca
     5461 gaagaaagaa caggaagaga gaagagcttc ctggaaagaa cacacaccgg aagtatttga
     5521 aaaaaagaga gaagaaagct atcgtgtcat ggaggcaaca aaatatgaag cggcttccag
     5581 ggaccggaga gaatttatac agaaacctgt ctggcaggaa aatcatgccg gggtacagac
     5641 cagtatccgc aagccggagc taatagagaa ctccacagtg ggtgttgcag gaactgtgac
     5701 ggtgaaaaaa acgcaggccg tcctgtacgg taatacacca gaagtttcca aagcagtcag
     5761 caccacagcc gtaccgcagg aagatgattt cttcgtggag gcagtgccgc cggaagacag
     5821 gaagaacccg gtacaggaac agatggccac aacaactgct tccggacagc ccacggcatc
     5881 gtcgcaggat gcagctgccg ccccggaacc ggagatcaaa aatacccagc agttgaatct
     5941 gttcgaggaa aaactgttgt ccatgcagaa tcgcagccgt taccggatta tcggtcaggt
     6001 ctttgatact tactggctga tccagtttga agacaaattg ttcatcattg accagcatgc
     6061 ggctcatgaa aaggtaaaat atgaacgcct gatgaaacag ttttatgaaa agagtgtggt
     6121 gtcccaacgt ctgatgccgg cgattattgt cagcctgaca gggcaggagg aaagcattct
     6181 gcacacctat gaggatgctt ttgcacagct gggatttgag attgatgctt tcggaggcaa
     6241 cgaatatgcc ctccgtagtg tgccagtgga tctctacgga tgcagtgaga gggaactgtt
     6301 cctggcagtg ctggatgaac tgtcccagga gaccggcaat cgcggaagtt ttcaggtcat
     6361 tgaggaaaag attgcatcca tgtcctgcaa ggcggcggtc aaaggcaata accgtctgag
     6421 ctttcaggag gcggagaaat tgatcgacga acttctgacc ctggataatc cctacaactg
     6481 tccccacggc agacccacta ttattaccat gacggagaca gatctggaga agaaatttaa
     6541 gaggatagta taatgcgttt actgatcaaa cagagagtgt tttcctggac agattcctat
     6601 gatgtctatg atgaaaatga aaatccaaaa tattttgtaa aagcggagtt tttctccctg
     6661 ggacaccagc tgcatgtata cgacagaaat aaccatgaac tgggagtgat cagacagcgg
     6721 ctgcttacct ttcttccggc ctttgaaatc tagataggtg gccgggtccg tggtgaaatc
     6781 cgcaaacata tttctaattt caagcccaga tatcagat
//
LOCUS       DAWDHD010000137         6754 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_656_length_6754_cov_13.831467, whole genome shotgun sequence.
ACCESSION   DAWDHD010000137 DAWDHD010000000
VERSION     DAWDHD010000137.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 6754)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6754)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6754
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_656_length_6754_cov_13.831467"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1748 a         1748 c         1423 g         1835 t
ORIGIN      
        1 cgatcatgat caaattcgcc gttcgccaat tatgcaagca tattggctca cttgcactca
       61 ttatatcatc ctccccgcat tgcgggaacg aatatcacat tacttctgaa agtctctgat
      121 cactttcaac gcgggcagtg catttccatc ataatcgaac agtgcctgat tggcccattc
      181 gtttccgccc ggaccgggat cctgaatata gttcagggat tcctcgttgg cccagccaca
      241 accaggcatt gggatccatg ccggctccca atagtagaag ccctttcctc tgccctccgg
      301 aacctgtgtg atcctctcca tgaccttctg cataaatgca gactgtcctt ccggtgtcat
      361 aggatactcc accgcctctg caaggtgggg cttggtcgca atgcccttgc gctgttccgg
      421 tgacagcttt tccctgtctg catagtcctc catggtgaag cccatggata cctctgccac
      481 aatgacatcc ttctgatatc tctgagagag atcgttcata ttatttgtca gatcatcaat
      541 ggttccatgc cagaaaggat aataggacaa tccgatgaca tcgaagtcct ctcctctctg
      601 tacataggca tcaaaccatt ctctgcagcg ggcattgttg ccgccacagt ccagatgaag
      661 catcacgcgg ctatccgggg atatttctct cactgcacgg atgcccgcac tgataaaccg
      721 ggcgatattg tcaacattgt cccattttcc atgaggccac atcaggccat tggtgatctc
      781 attacctacc tgtaccatct ccggaagcag atccagacgc tttaatttca gcagcacatc
      841 cttcgtgtaa tcatacacat acttctccag tgcattggca tctgcatacg cccactcctt
      901 gggaggaaac tgtttgccgg gatctgccca gaaatcactg taatgcagat ccagcagata
      961 gctgtaaccc agctttttgc ctgcggaagc cagtgccacc agttttgtaa agtcactggt
     1021 accggctccg taaggcttgc cgtcctcact gtaaggatcg ttccagagac ggatgcgcag
     1081 agagttggca ccgtattttt tcaggatctc cagtgcgtcc ccctctttgc cttcgtcata
     1141 atactttgca ccgagagctg tctcttccgg caaagtcgat acatccatac ctttgataaa
     1201 tttatccata aattaactct ttatctccat gtcatacatt tatccggaga atccattcca
     1261 tggattctcc taggttaaac tgcctaagca gtttaacctt tcactgcgcc accggtgata
     1321 ccttccacat agaacttctg catgatcaca aataatacag agataggaat agataccagt
     1381 acaccgcctg cacagaactg accaaagtaa gtgttgatca ggctcttatc cagcatattg
     1441 tacagaccga tagcaacagt ccactgggaa gatatgccgg catttaacat cagctttgca
     1501 tatacgaaat ccatccaggg ggacaggaag gagctgataa cggtatatac aatgatggga
     1561 cggctcatgg ggataacaac cttccagaag atcgtagcct cactggctcc atccagataa
     1621 gcagcctcgc gcagggaatt cggaatcgta tcgaagaagc cctttgcgat caggtaaccc
     1681 agtccggcgc cggcagaata taccaggatc agtccgaggt gagtgttggt caggttcaat
     1741 gtcttcagga taaaatatac tgcgatcatg gacaatacac cgggaaacag attcacgata
     1801 actgcggcgt tcatcagcgg cttacgtgcc gggaaacgca tgcaggaggt cgcataggcc
     1861 accatcagta caaaagcggt ggagatcaca cataccacac atgcaatgat gaaggtgttc
     1921 ttgaaccagt tcgggaactg tgccacactg tctgctgaaa aaagcagatt cttatactgc
     1981 gccagactcc attccttcgg gaaaaaggtg ctggtattca tgccggtgta tgcgctgaag
     2041 gaggtgacca ccagccatac gataggcacc agccagacaa atgccaaaaa cgccagtaac
     2101 aggttatgta ataaagtctt aataaatctc ttcattaacg gaacgcctcc tctctgtcac
     2161 ccttcaggaa tctgctgaag gcaatgaggg taaataccgc acataggata aacacaataa
     2221 taccgatggt ggatgccatc ttgtagttgt aatagctctg tgtcagattg aacagccagg
     2281 tgatcagcag atccacttcc ttcgcctggg agttcgccat tgcctgattt gtggtggtat
     2341 atacaccctg ggtcaacaga tagattacgt tgaagttatt aatattcttg acaaaatccg
     2401 ttaccagaga aggacccgtg acgaacagca tatacggcat agtgatatga ataaagctct
     2461 gcatcggagt agcgccgtcg atcttcgcac tctccagctg gtcagaagga atattcatca
     2521 gcacaccggt agcgatcagc atctggtaag gaacaccgat ccagatattg atcaggatga
     2581 tcatgacatg tgcccatccg ggagcggaca aaaacggaat ataactggta ctgatcgcgc
     2641 cgatatctct cagccactgg gtaattcccg cattggcaca gattgtgttc atgatgccgt
     2701 tgttcgcgaa aaagttacgt accagaagta aggagacgaa ctggggaact gcgatcgtta
     2761 caatgaacag cgttctccac agcttgggca atttggttct cttattattg atcagcaggg
     2821 acaacagaat accgccgatg taagtggtag cggttgcaaa caatgcccat accagcgtcc
     2881 agatcaagat cctacggaag gcataaccga aggtaatggt cattccaccg ctggagaaca
     2941 gattcttgaa gttggtaaat cccacccatg taaacaatgc ggagggaggc atatgtccct
     3001 gatcatagtt cgtaaacgca acagcgatca ggatcagcag aggaacgatc gtgaaaataa
     3061 caacacccat gacaggaagt gccagcagtg tgatatagaa tttgtcattc ttgaactgct
     3121 gcaagtcttc tacaaacgta ttgatatgtt ctcctgcctg tgcttttttc tgaagccggt
     3181 aaacggtcac ttcattctta agccaaatca ccaggaatgt gaaccagacc acaaatgaga
     3241 ttacggagaa caataggatc ataaatgagt tgtcataatc attgaactca ttctgcatgg
     3301 ttacaatatt gaaaaccgtc tcctgctgaa ccgttcccaa agttccaaaa tcacgtacat
     3361 actctgtggc aaagccaaag gtgaatacaa tgactgccac ttcgagcaga gtcattaaaa
     3421 tacctttgat aatttgtttt ctgcggatat atcctgctcc catccagacc agggataatt
     3481 ttacgaacag atctcctttt acgactgctt caccgaactc cttgaagaat ccgccgattc
     3541 cgctcttttt cttcttcatt ctctgccctc tcctttcttc atattaatat aaatttttag
     3601 taactgttcc gaacatcttc ccacgttcat ctgaccagtt acataacccg gcaggcagga
     3661 gtcgaacccg cttaccggaa acgtttcctg taaaaaaggc atccctccac tacgtattat
     3721 tgttcatagc ttcaggacgc ctttctattc tctgtattcg ttctgcacca gaacgttttc
     3781 atcattcatt tgtcacagaa ccggaacaga tcatcttccc aaacttattc agcgggtacg
     3841 gaaacacctt ctaccagctc atccagggct gcctggatac ctgcttcatc atcagccttc
     3901 agctgaccct gagctacctt ctcaccaaaa gtcttggaag gatcccagta cttgccgcca
     3961 acgatctgaa ctacaccata agcggactgc tcagacagag ctgcaatagc ggtattggaa
     4021 gaaacctcct cggaaccagc tacattgata ttggaaggtc cgatctctct ctctgcgaaa
     4081 cgtactgcct ggttgtcttc gttggtgatc cagtcagcca gcagagttgc ccagcctgca
     4141 tttgcagaat acttgttaac acctaccagc ttgaagcctg ctacggaacc ctgctgaatc
     4201 tgcttgtcac cacaggtata ggtaggaagc ttggtagctg cgtagccttc accgaaagcg
     4261 gtctgagctg cagctgcatc ccaggtaccg gaaactactg cacacagttc gccggaagcg
     4321 atctggttag aaacatcacc atcagcgatc ggaaggaatg cgctgttgcc ggcgataccc
     4381 agcatagcct gtacaacctg tacaccggta acaccatcag cgctggtgcc gttccaatcc
     4441 atagcagtac tgccgtcatc gttcagtgca gtagtaaatc ctgcgccata gaagaaggaa
     4501 gcattgtacc aaccggaagc aaaagtcata ccaaccttct tgccagcctt ctcagctgct
     4561 gcaagtaagg tatcccagct ctgtgcatct tcgggagtta ctaactcgga attgtaatat
     4621 aagaagtaac cgttatcagc actcataggg aatgcataca gtgtgccgtc cttggttgct
     4681 gcgtctacag aaccgggagc gtttgcactc ttgacatcag ctacgctctt gccagcgtaa
     4741 gcttccagag ctgcgtccat atcatcaaca gactgcagag cacctgcatt taccagatcg
     4801 atcagctgat cagatgcgaa agaatacaca tcagcagcag cctcaatatc ggttaaaaca
     4861 gtatccttag ctgtggactc ggattcggta ccaagctgga tatcgaagtt tgcataatct
     4921 gcgtaatgct ccttgaagga atcgatcatg ccctgtaaca tgggctgatc ttcctcagca
     4981 ccccacattc tcaaggtaac gtcctgcttc tcagcgggag cggtctgctg tgcagcctcg
     5041 gtggtggttg ctgctgcaga agttgctgtg gtatctgttc cggcatttgc agaagcatca
     5101 gagtttccac aagctaccag agagaatgcc atagtcagtg acattgctaa agcgactaac
     5161 tttttcattt tgttatcctc ctgttttgtt ttgcaagttt gtgcgtattg cgcaaccgct
     5221 tgtctacgat aatagtttag caacatttcc ttatgttgtc aatttataat aatgactgat
     5281 attgagatct aaaattgtgc actttgtaca agtccgtttt gaaaccattg attttactgg
     5341 gttttctgta tatccaattt cttcaaaaat tcaattttta cccttgtatt ttgaagtttt
     5401 tttggaaaac gtttttctaa attatgtctt gtcatttcaa gatcagccct tcgtggactc
     5461 tttcaggatg atttcatagt ccagaagtgt cttgtccgga accgttttcc cgtgaatcat
     5521 gtccagcagc acctgacagg atctcctgcc tagctctgtg atattgaatt tcagggaagt
     5581 cactgtaacg ggatagttct ccaggatacg actgtaatgg caggatgcca ctttcatctg
     5641 atccgggatc cgcacaccca tgtgagaaag ctttcttacc gcctcatcgc agatgtagtc
     5701 atcctggcac aaaatgcact ccacaccgct ctttttcagc tcttccacgg ccttttccac
     5761 catagccttg ctgctcaagt ccgtgtacac cagatcctgg ttcagcttgc gctttccctt
     5821 gcggtaggct tcctcatatc cctgatacct gcagcggttt accatctgat ccatattgct
     5881 tcccaggtag gcaatattct ggatcccttt ggcgaacaga accgtcacca gttccttaca
     5941 ggctcccaca ttatcatggt ctacctgtac cacatcatca tccgggaatt tccccacagc
     6001 cacaaaggga atctgcttct gtttcaggaa tttaacgtat ttgtcgttct cataagttct
     6061 ggtaagaatc attccgtcca ctttccggtt ctggatcagc cgctccaatg gtttggtatc
     6121 gtttccgttc gtcgtgacca cgataatatc ataatcattg gcctgtgcca cttcttccac
     6181 gccatacata caggtatgga aaaaaggcat atccacaagg gtattgacct ccggcaggat
     6241 aatggcgata ttattggtct tggattgtgc caggctctgt gctgccgcat tgggataaaa
     6301 gccatgttct tctatgtagt cgagaatccg gtctctggta gcggctccaa tacggccttt
     6361 tccggagatc gccctagaaa ctgtgctggc agacaccccc agttccctcg ccacatcatg
     6421 gatcgtcatt gtcttatcat tcccagaatt attgttcata ccttcatcac gcctttccgt
     6481 gacctgcaac ctacagattg caggcacact ttcctcccca tccttgcgca atccgttatt
     6541 ttcatacctc tttattgtca caatatgcgc aaacgattgt tcttgtttat agtatcatct
     6601 tgtgatgtag ctttcaatgc atctttttac acaaattgcc tcctcatttc tatacaatag
     6661 ttacaaaaca atcaagtttc ttgaagttat ttaacctgtt ttgccgcatt ttgttccgga
     6721 actgtttcca atctcattct ttatgatact aatt
//
LOCUS       DAWDHD010000138         6717 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_660_length_6717_cov_15.864305, whole genome shotgun sequence.
ACCESSION   DAWDHD010000138 DAWDHD010000000
VERSION     DAWDHD010000138.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 6717)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6717)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6717
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_660_length_6717_cov_15.864305"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5337..5436
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1608 a         1707 c         1393 g         1909 t
ORIGIN      
        1 agtcatgaca caagaaaagc tccctcacag gaaggagctt tgtctacagt ctgagcctga
       61 ggcttcttta gaagcactca ggctttatca ttctgaaaac tagatctgga actgatgcat
      121 cagctgtaac atagtatgta cctgcgcgga ctgttcttcg ctgacagcgg cggtctcctg
      181 agaagatgcg gagttgttgt ccacgattcc ggccacatga gcgatgtttt catcgatctg
      241 ctggacgctg gacacttcaa cctgaagggc ctgtgccata tcatccatac gcttggtaga
      301 agcctcggct tcttccatta cctgatccat gctggcttcg gtttcttctg caatggcgat
      361 tcccttgttg acagcatcga tggtgctctc gatcagcttt gtggtctctc cggcagcctc
      421 ggtggactgt tccgccagat tcttgacctg ctcggctacg acagcaaatc cgcggcctgc
      481 ttctccggca cgggcagcct cgatggaggc attcagtgac aggaggttgg tctgggaagc
      541 gatatcctca atgacctgaa tgatagaacg aatctcctcg gaacatttac tgatctcacc
      601 gattgcgact ttcagttcct gcatcttggc attgccatca gcaaccgtct gtgcggactg
      661 cttggagata tctgcggtct cctgtgcaac tctggctttt tcttcaatac tctttgccat
      721 agcgtcgatc atctgggatg cttcggaaat cttggaggcc tgtgcggtac agccttctgc
      781 cagatcggta gctgcctgtg ccagctgctc ggaaccacca tcgatctcct gtgcgctgac
      841 ctgaatggtg gacagtgtct tcttcatatc ggcaatgatc ttcaccatgg aatccttgat
      901 ctgtacaaag tcaccgacat attcttccgt aggctctaca cggtaattgc cctgacccat
      961 ctgacctaag atctcggaaa tctcggtgat catcttggta aagttgtcgt tcatgaaatg
     1021 aacggcctgc accatgatgc cgacttcact gtcatcctcc ggcagatcca gacggctgtc
     1081 aaaatgtccc tgcgcaagta ctttcatctg ctcggatact tttacaatgg gaatcagcag
     1141 ttcctgtttt gcaaaggtca gtgtggtgac aatcttgcgg gcaatggtca ggaagcagag
     1201 aatgaaaatc accatggtag tgatcattat cagattcaga gtattttgct tctgagccat
     1261 acgtgcctgg atatcattga tacaattatc tgtggttgca gtgatctcct gtacggtaga
     1321 ttcatattcc tcaccaaata cataagaaat agctgcttgt gtatctccgg aaccggcctt
     1381 atccatggct tcttcttcca gaggaaccag accgttggac atttcagcaa tatggtttaa
     1441 cagtgcccat tcattgtcgg tcagaccgtc tttctgaaga ccttcccagg caatgtcacg
     1501 atttttgtcc tcgtttaatt ccttcatata attgtcatag taggtctggt ctccggtgac
     1561 tgcgtaagac tgtacggcag cggtcagcgt cttggaaccc agacgatact ggttcagata
     1621 catggtagct tccagctgct gggaattgat ccggctcaga tacatgttgg atccgatgaa
     1681 caggatcagc ataacgatgc tgatgatcag tatccgatag gttgatcttg tgttttgctg
     1741 cagcatggca gactgactgg ttactttgct ttgctttttc atttgaaata taacctcttt
     1801 tctgtaatac atggtgaact gatgtgttac gaaaattttt tcctacagta tactacaatt
     1861 atcggaatta tacaaatctt gcgccttaaa aaatacagca aaaacgcgta aaattattta
     1921 tacgaaccat cagtgacgac ttacacccta tacaggaaga tggtactata aaaagtaaga
     1981 ggcatgattc ggaaaagaga agataacggt aaataaacag actgtagaca aagctccctc
     2041 acaggaagga gctttgtcta cagtctgaag aattgtgtca ccacaattcc ttttcctcgc
     2101 aacaaacatt ttacaacaaa cgttcagacc gcgccattcg caaagtcgca ttatcacgct
     2161 ctccgtcgct ttgtgtgcaa gcacccaatc gccggttctg tcattctatg gctgaactat
     2221 tattacaagc atttaataaa acggtcgaaa gccttctgtc cttcctcggt tctcttgtag
     2281 acaccggcat cttccaatac ctgcatgaat accagaccga tctctttctc gacaataccg
     2341 tcgatattct ctgcagtgat agcatcgtat ttcgggcgga actcgtctac ccagtctgca
     2401 tgctttgcca gttcctcatc ggctcttaag tctttaccgg cgaggattgc ttccttcaga
     2461 tgcgccatct cgcccttcag gcgggcaggc agtacggcca gacccataac ctcgatcagg
     2521 ccgatattct cttttttgat atgatgcagc tttgcatggg gatgatagac tcccagagga
     2581 tgctcctgtg tggtgatatt gttacgcagc accagatcca gctcgaactt gtctcctctc
     2641 tttctggcaa tgggagtgat ggtgttatga ggttcattat cggtgtaggc atagataaat
     2701 gcctcttcat ccgtatattc tctccacttg ttcaggatca catctgccag tgcaataatg
     2761 cggtcggtat ccataccgct gaggcggatc acagacatag gccatgccac gataccggca
     2821 tccacatctt cataacctgc tacagagaag tttttctcta caggcgcctt ggccatggcg
     2881 aactcataat gaccaccctg gaaatgatcg tgacttaaaa tagaaccgcc tacgatgggc
     2941 aggtccgcat tggaacctac aaaataatga gggaactgct ttacaaaatc aaacagcttg
     3001 cagaaggtcg catgctcaat cctcataggt acatgctgag aattaaatac gatgcaatgc
     3061 tcattgtaat atacatacgg agaatactgg aatccccact ggctgccatt gatggtcacc
     3121 ggaatgatgc ggtggttctg gcgggcggga tgattcacac ggcctgcata accttcattt
     3181 tcacggcaca gcaggcattt cggataaccg gcctgttttg ccagcttcgc tgcagcaata
     3241 gccttgggat ccttctcagg cttggacagg ttgatggtaa tgtccagatc gccgtatttg
     3301 gtgggtgcta cccatttcat atccttgcag atacgataac ggcggatata gtcggaatct
     3361 ctgcttaatt tgtaataata gtcggtagct gcctcgggag atacctgctc gtacagttcg
     3421 tggaaatgac ggatcacttc actgggtctg ggcatcaaca gtcccatgat cttcgtatcg
     3481 aaaaggtcac ggtataccac actgttctcc ttgagaatgc ctttttcata agcgtaatcc
     3541 agcatgccct tcagaacctc ttccagttca tcctcacgca tggtcacatt gtcgcgctct
     3601 tccagttcat ccagttcaaa cagctccaac agacggttgg tcgtgtagat cacatcttcc
     3661 tccggaacca gtcctgtctg tactccgtag gataccaatt tacgaattaa tacctgaata
     3721 tccatttttt ctccattctg caataccgga tttctccggc atcgcactct ctattctcgt
     3781 cggatcttaa tttcttacag ggtctggggg ccgtcaccga tctctactac atagaaatca
     3841 gctgcataac cgatcttctc cttatatgcc ttgcctacct gatcgataaa ggtgtcaata
     3901 gcctcatcct ttacaatgct tacggtacat ccgccgaaac cggcaccggt catacgggaa
     3961 ccaataacac cgtctacctt ccatgcttct tctaccagag tatccagctc gatacctgtt
     4021 acctcataat catcccgcaa agatacatgg gaagcattca tcagctcacc aaacagcttc
     4081 acatcgttgt tcttcagagc ttctaccgca cgaacggttc tctgattctc atatacagcg
     4141 tgctttgcgc gctttctgcg gacatcgctc ttgataatag ctttgaactt ctcgaaagtc
     4201 tcctcgtcca gatcacccag agtcttgatc ttcactacct gctgcagttc tgccagtgca
     4261 gtctcgcact cgctgcgacg ctcattgtac ttggaatctc ccaggcctct ctttttattg
     4321 ctgcatgcga taacgatctt cgcattttcc agatggatcg gtgcgtactc atattccaga
     4381 gttgcggtgt ccagaaaaat agcattatcc tttttgccca tggcaatggc aaactggtcc
     4441 atgataccgc agtttacctt gttgtatttg ttctcggaga actgtccgat cagtgccaga
     4501 tcctgattgg tcacatcaaa tccgaagaaa tctctcagaa tgtagccggt cagcacttct
     4561 acagaagcgg aagaggaaag tccggaaccg ttgggaatat taccgttcag cagcagatcc
     4621 ataccggaag tcactttcat gcccttctca ccgaacgccc agatcactcc cttgggataa
     4681 ttggtccagc ctgcttcctt gctgggtacc agatcctcca gagaagattc gatcactccc
     4741 agatgtccga aattcatgga atagaaacgc agcttattgt cgtttctctt tcttgccaca
     4801 ccataggtac cgatggtcag tgcgcagggg aaaacgtgtc cgccgttata gtcggtatgt
     4861 tcaccgatca gattcacacg tcccggtgca aaataagcct tggtgccctc tgcatctcca
     4921 aatacttctg cgaaagtctt cagaatcgtc tctttcattt ccatttttct actcctgtct
     4981 gcttacgatg taagccccgt ccatttatat gttcttactc ttgtcgctcc ggccgatgcc
     5041 gcgatacgaa ttctcttgct ctatgttttc atcataatcg aaaaaaacaa atatttctac
     5101 ctatttttgt ttttaagacc tgtcattatg ttaaaagcta caagccatgc aaaaataaaa
     5161 agaaccaaca ccgtgagagc ttgctcgtga aggtgttgat tcttttttat ttttatacgg
     5221 cgacagccgt acatgaagca ggtgctttag cgcctgcgaa tgcgcatggc gcagctcctt
     5281 ggagcttgct cgtgaaggtg ttgattcttt tttattttta tacggcgaca gccgtannnn
     5341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnagca ggtgctttag cgcctgcgaa
     5461 tgcgcatggc gcagctcctt ggagcttgct cacaaaggtg tatctttctt tttggattta
     5521 tacggcgaca gccgcatatt cgcaggcact tcagtgtctg cgaatgggta tggcctaatt
     5581 cttatttctc atctttattt ccaaacagct tctctgctgc tccggccgcg cccataatac
     5641 catttacggc agggttcttc agtgcaccgg ctaccatgtt cacctgagta gcggcatccg
     5701 tcgctgccag caggctgtta cgatactctt cgttgacttc atgtgtggaa gcatactcaa
     5761 tatagcttgc cagcatatcg aaatctttgt tgctgtcata tacaatggct actaccacat
     5821 acagaaccac acaggcaatg cccaatacaa caaacacaac atttttcagt atgccgccca
     5881 aagctacaaa taccagagta ccgatcacac tcagggttac cagcaattca gatacagcgg
     5941 catcttttac ggattttacc agaaaagtct tgccatcctg taacagcaga gatacgccgt
     6001 cataataagc ctccttggtc atctcgccat attttgcaaa cagataaccc tctaacaaca
     6061 gattgattct tctgtaagct ttcttgatca tactctgtgc aataccggat acagacttca
     6121 gtccggggat cttctccagc atggctgccg cggtgaaaat accctggcac agatgatata
     6181 aaatatgaga gaactctctg gtctggctct taagtgcaga caggtttcca ccttgcttct
     6241 ttaccagttc aataccggca tttaccacat cctcgggcat ctcttcaccc atcagagctt
     6301 ttgctgtcat aacacgtttt gcatactcgg tatacccgtc tacccagctg aggatcacta
     6361 atcctgcaaa aaatgctacc actcccagca gggtcaatac gttgatctgt gtcatcagtc
     6421 cgatggctgc cacaatgaca cctgctgcca tcacgccgta ataaattccc agtcgctggt
     6481 tgaatttctt ttgtactgcc tttgctttct ctctgttatt cattttcttg tccctccata
     6541 agttgtggtc ttattctatg accatatact actttagtat aactttactg tcataaattg
     6601 tcaatattca gcatattttt atacaatgtt aaaattatgt tatactttgt ttacccgata
     6661 atcaatcgag taggaggcgg tgattaaccg ccgtcctctc acaccaccgt gcgtacc
//
LOCUS       DAWDHD010000139         6690 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_664_length_6690_cov_9.078975, whole genome shotgun sequence.
ACCESSION   DAWDHD010000139 DAWDHD010000000
VERSION     DAWDHD010000139.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 6690)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6690)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6690
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_664_length_6690_cov_9.078975"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1707 a         1663 c         1346 g         1974 t
ORIGIN      
        1 cgtgccgcgt ttcttatacc ctgccaaggg ctacggggat ttgatagcat ttcttaacta
       61 actttccatt tactctacag aaacagtata gtaataaata ggttgtccgc catactgcag
      121 ttcaatatcg catgcaggat aagcctcttc cagatctgca cgcagtgctt cagcagcatc
      181 ttctgccaca tcacagccat agtatacgct gatcagttcg gaatcctcat ctaccagctc
      241 ggcgatcata tcacgggtca cctttaccat atcggtgcca acagctacaa taccctgatc
      301 accgataccc atgaagtcgt cctttttgat ctccttgtca tcgatcacgg tatcacgtac
      361 cgcataggta acctggccgg tcttgacatt cttgatctca cgaagcatgg tctcttcatt
      421 ctcttccaca gacagctcag gaacaaagtt gatcacagca gtgataccct ggggaatcgt
      481 cttggtggga atgactaaaa gctccttgtc agtcatcagt tccgctgcct gattggctgc
      541 cagaatgatg ttcttgttgt tcggaagcac gaagattgtc ttcgcattca ccttgtctac
      601 tgcatccaga atatctgcgg tactggggtt catggtctgt ccgccctcga tgatataatc
      661 cacaccgagg ctcttgaaca gttcactcag gccgtcacct acagagactg ccagaaatcc
      721 tacttccttg ggaggctgtg cagccttctc ttcttccgct ttcttctgcg ctgcttcttt
      781 ttcggagagc ttgaacagct tctcctgatg ctctaagcgc atattgtcga tcttcatgtt
      841 ggataatgca ccatacttca atgccttctg gatagccaga ccgggatcat tggtatgtac
      901 atgtaccttg accacatcgt catccgcaac acatacgata gaatcaccaa tggactctaa
      961 aaatgccttg aaatccatct ctgcttttac gttgaaaggc ttatttaaca gaatgatgaa
     1021 ttcggtgcaa taaccgaact tgatctctgc ctctgcctgt acttccagag aactcttggt
     1081 atcttctgcc ttcgcagtag ctgcgggagc tacagtcaga tcgatctcct tacctaagaa
     1141 tgcgtcatac gcaccactta aaacttctac cagaccctgt ccgccggaat ccactacgcc
     1201 tgcctgcttt aatacgggca gcatctcagg cgtctggctc agtacgtaac gggcatgttc
     1261 gatcacagat cctaaaaact gctccagatc ctctgcacca tcttcacaca gctcctttgc
     1321 cttatcggaa gcacctcttg ctaccgtaag gatcgtacct tccttcggct tcataacagc
     1381 cttgtatgcg gtctccaccg ctttatcaaa tgcacgggtc agaacaggaa tatccagaac
     1441 ctgctcttcc ttgatgctct tggtaaagcc acggaacagc tgggacagga taacaccgga
     1501 gttacctctg gcaccacgca gggaaccgct ggaaatagcc ttgcacaggg cagccatatc
     1561 tgcatcctcg cccagggcgg acacttctct tgcagcagac atgatcgtca tggtcatatt
     1621 ggttccggta tctccgtcag gaacggggaa tacgttcagt tcattgatcc attccttttt
     1681 attctctaaa ttcttggcgc cggctaagaa catcttggaa agcatcttag cgtcgatttg
     1741 ttgtaaagcc acaattactt cctccttaat caatcactct tacaccttca acaaagatgt
     1801 tgatcttttt gatctcaata ccggtaaact cttccacttt gtattttacg ttgctgatca
     1861 gattgtcggt gacagccaga atgctgacgc cgtaagctac gatcacgtgg aagtccagaa
     1921 ccagtttatt gttgtccagg gacacattga tgcctctggt gatgctgtcc atcttaagca
     1981 gctttaccag accatccttc atgctgactc cggccatacc tacgatacca aagcactcta
     2041 ccgccacgct gccggcatac tgtgcaatga cttcattatc aatgacaata tttcccatat
     2101 gggtattcat agaagaacct ttcatataga acctccttca tattcggatc acttgtttct
     2161 tccgatatgt ctgcctgaaa atctacagtt cagacatata taaatgtatt ttaacagctt
     2221 tcttttgaaa aagaaacaag aaaataaaaa aaataaatat tttttgcttg cttttatttt
     2281 ggttttctgt taatatatca atgttgtgag catttcacat gtattttatt gaaaaaaatt
     2341 gtttaaaagt aagcgtaaga tgcgcgttag atcgaggagg tgtcaagatg gctaaatgtg
     2401 atatttgcgg taagggcgtt cattttggta acaacgtaag ccattctcat agaagaagca
     2461 acgcaatttg gaactctaat gttaagagcg ttaagtgcaa ggttaacggc ggagctagaa
     2521 gaatgcatgt ctgcaccagc tgcttaaaat ctggtaaagt tgaaagagct taatcttctg
     2581 cgataatatt ggattttaaa aagccagaaa tgaaattcat catttctggc ttaatttttt
     2641 gcttgtaatt ttccatgcag ttaccagaac cgctcttcct gtttccgttt cagatattcc
     2701 ttgttgatct gtccggccaa ttcaccgtca ccgaacaggt tgtccggatg aaagatcttg
     2761 atcagatccc ggtatctctt ccgtaaagcc agcggattat tgactccccg gaataaaagc
     2821 tgtaccgctg taaaactgcc ttctccggga tcctgccctg tggcctgctg ttctctggct
     2881 tcctctatga gtatctgccg ttcctgttcg aacttcttcc ggtcttcttc cagctgtcgg
     2941 aacccctcct gtaaaatggc cattttcttc tcaaaaaaca gattctcttc ctgcagccgt
     3001 ttccgttcca tgattgtacg gcggtttaat tcatccagct cgtcccgcag tttcacccgc
     3061 tcatccagaa atttgttccg ggcctgttcc agttccgtac gctcctgctc cagctctgag
     3121 cgttcctgtt tcagacgcat attttcctgg aaaagccaca gttttatttc tttcagttct
     3181 tcggtactct ctgccttaat gacctcttcc caatcaatca tactgcaccc tcttactcgc
     3241 aaccacagaa aagataatac cctgcaaata ataaaagtgc taccatgatc aagcctataa
     3301 aaccgttatg gatcacaagc atgagaagca tgcatacatc cacaaaaaag agtatcagac
     3361 caatgatctt cttcatctat caaacggatc cagactgttc tcactttatt atatgcagag
     3421 tattttaaat attacttgtc ttctgtagtt ttctcttcct cttcaggaaa tgcgatatct
     3481 accacttccg catcggacat ttcttctttt tccttcggct gggtaaattt atctacgata
     3541 tcgggaatca tatccagaat cttggtgact gtatcctgat tcttgatatt taccagcttg
     3601 gtggaaccgt ttttgatgac cagaaccgca ctgggggtca tcttaccgcc taagcctccg
     3661 gctccggatg cggatttttt ctcggaatta ttgccggctc cggcaccgac accaaaggtg
     3721 acatccacaa gcggcaggat gatcgtatcg ccgatctttg tagcctcacc gactacggtt
     3781 ttagtggata atacggtatc catacccttc aacagggatt ctacaacgcc ctgaaactga
     3841 ttctctttgt ctgccataac aaacctcctg ttttacactg ttattttttc tgtgtcttct
     3901 gatattgtct gattttatgc aacaattccc gtaatcgccg atctaaaagt agtcggacgg
     3961 aatggaataa tacacaggcc gcggtaaaat gtccggaagc cttgaaatcc ccttccagaa
     4021 tcgtctgttc aaaatccggg gtaatgcaga tcccctttcc cagcttcgga agcaccattc
     4081 cgtagatacc atacagatat cccgtagtat ccggagaacc ggtgccgacc gtggcattga
     4141 ccgtaagctt tctcgggcgc agccgcttcc agatatgtcc gaaacgtttc ccggcatgtt
     4201 tcaaaagtcc ctgggtatcc gggtcattcc acaggttctt gtaaaatgca atgttttcca
     4261 gaatattttt gattttatca caaatctttc ggattgtgta ccggattctt gtaaaaaatg
     4321 cgtacagacg atccgtagtc ttcccgggag cttctcccgc tggctctggt gttcccggtt
     4381 ccgggattcc ggaatcctcc ctttccgcag cagactcaac atctgcggcc gttgccgaga
     4441 atgccgtggg atcttccgct tcgatcagct taggcgtccc gacagattcc ggggtatgtt
     4501 cttctgttgt ctgctccgca gaagcgggtg ttttctcgtt ccctgtcgtt tccgtcgatg
     4561 tccggtctgt tttctccggc ttctctacgg aagaatcata tagcgtaaag aataaaaact
     4621 ttacgcaaat tttcccggga tcaggataat cgcagttcat ccgcaataga ccgaacaacc
     4681 attttactct cgcggtgatc cagaattcct ctgccgtccg ctttccggca gctttgtaag
     4741 aaatcggaaa gaacagaacc aggagaatta ccaccagtgc gattcccaac aggatcagaa
     4801 gcacgatccc aagaatactc aatattttaa aaagaatcgt caacatagta aatactcccg
     4861 cagattacag tcccctctgg ctttcaggca ctctccggtg atccgttcga tcagctgcag
     4921 cgcatcctcg tagccggaag ccataccgag aacgagaaat tcttcgtcct tataatgagg
     4981 ctgtaccaac tgtctggcat caaaaatatc cagctgatcc gcgggatttc tggccggagt
     5041 gatcagatat acattggcca gaagaggctt tttattcagt ctctttttta gtttatccag
     5101 tttttccggt gcgatgccct ctccaagata gagattttgc gcaaacttca gctttcctgc
     5161 catacacaag ttccttaaat ctttttctcc tagtatagca tagtttcctt tatattacaa
     5221 ttttgttact tgacttttta gtatgatatt ttatacttga attagtacaa atgaggagga
     5281 tacagccttg aaggttatta ctaaaatgga gttacagccg gatatggtcc tgggggagga
     5341 cattctggat caggatcgtg tgatctaccc tgcaggaacc accatcactc cgcagatcat
     5401 tgagaaattg aaacggtaca atctggtatg tgtcaccata atggaagatg ttgatttcgc
     5461 cactacacat tatgccaaga ttcgttttga ttctaatttc aaagcattcg aacgtgctta
     5521 tccgttgttt ttgggacaat ataaattagc catgaaacaa ctgctgatca tgggaagaaa
     5581 accggcggat attattctgc tgacaattta caacgaattg tattattata ttacttccgg
     5641 cccggtactg ttagactatc tgtacaatct gatgcccagt gaggatgaac tcacctatac
     5701 ccagggactg aatgccgctc ttctggcggg gaccttcgct gactggctgg gaatgagcga
     5761 agaagagaaa aataccctga ttctctgcgg tttctattat gatatcggca agctgcagct
     5821 gccctatgaa ctgttatgga agcccggcaa actgacggat gaagaattca aagttatcaa
     5881 gactcatcct gtagtcggtt ataccaccgt acgtaatcag gatctgaatg agcatgtgaa
     5941 aaatgctgtg atcatgcatc acgaacgact cgatggcagc ggctatcctt accatatgtc
     6001 cggacagaga attgatgtct tcgcaaggta tattgccatt attgatgcct atattgccat
     6061 ggcgtctccc agaacctacc gtaatgcgtt gacaccgtta cagattcttg ggaatttcga
     6121 aaagagtctg gataaatatg acgtggaatt attgatgccg atcatgaaac gtattgcaga
     6181 tgcccagatc ggaaccagcg tggagctgaa tgacggaagc atctgggaag tactgatcat
     6241 tcatcccaac aaatactccc gtcccatttt aaaaaatgat aaaaacgaat ttgtagatct
     6301 cttgcagaga ccggatctgg aaatcgtcaa gaatgtttaa aaatatttca gaaaagcagg
     6361 aataatcaat ttttccattg attattcctt tttctttcct gataatccgc ataggctttt
     6421 tccagaatct gcttttcatt ggcaaatttc agaagatggt atgcttcatg atatttgtaa
     6481 aagccggaat ccacaaaata ctcttctctc tggggcttgc tgtaatagct gtccgttccc
     6541 atcagatacc agtggacttc cttctccacc acatcttctg gtacggtaaa agaatactcg
     6601 cttctgccga tataatctat aatgtatgcg ttggatccgc tttcctccat cacttccctg
     6661 agtgcggtct cctcatgggt ctccccggct
//
LOCUS       DAWDHD010000140         6599 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_668_length_6599_cov_10.359719, whole genome shotgun sequence.
ACCESSION   DAWDHD010000140 DAWDHD010000000
VERSION     DAWDHD010000140.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 6599)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6599)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6599
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_668_length_6599_cov_10.359719"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1697 a         1737 c         1532 g         1633 t
ORIGIN      
        1 tcagaacagc agcaagatcc gtgctaccaa cgttactgtt gtagcttaat agcaagacag
       61 accaaaagga tcagtcccac gggactgatc cttttttgtg tatacgatga gccaagtaca
      121 gatttgtgct tgcacaagaa tctgtatttg gcgaatgtaa atcatcgagc ttttaagcga
      181 gatggtgtac aaagtataca aatcgagtag gagccagcct actcgattag ctattcaaaa
      241 ttcatccacc gctctgcaga aatctccggc ttcgtcacaa ggcattccgg atccggtgcc
      301 agcacatgcc gtaccacccg gaacactttc tgcgcatcac acagcagaca cttcgcatct
      361 ccgaacatca ccacttccac tgctccacaa tccagaaacg cctggttcaa tttcacaaaa
      421 tcttccgaga tatggggctg gtcgggatta tagtgttttc tcatatatac tttcttttct
      481 cttccgtccg gcaggatcgt tgtcaataac atgggctccg ccgccagacg attcggaaca
      541 ttcagcacct cttccacact gtggatgtaa gtattgcgtt catgtccaac gccaaccaac
      601 agaatcttac caccgcagtc tttcagccgg tcgtaggctc ctcccggggt acatggagtg
      661 ttacgttctt cctctcctgc cagatattcg gcagcatttt ttccgtaccc cgctaaagaa
      721 tgggtgggat gcagggagcg caccacaccc tctctctgcc ggaacagatt ggtcagcagt
      781 cccacgcagg atggggtatt acgatagtca tacaccgggt gctgtgcatt cacattggcc
      841 caggtatggg tcggcaatag cagaaggcct tccgcaaaat attccatcca ggcatccagc
      901 acgatatccg caccgccctc cacttctccg atggccttca tggaagaatg gatcaggatc
      961 gtctcttttc ccgtaagtcc cacattccgt aactgctgtt tcaattcttc tttggtatac
     1021 atattttttc ctcgcatatc gcatatagta aaaggccggt ttcctgggga gcatggaacc
     1081 gaccttttta tgctgttttc tctactttcc cgcttccggt acaaaactgt cgaagatgaa
     1141 aatatcgaaa tgttttctcg cttcgtccag ttccttctgg ctcttgatgg tccatgcggc
     1201 tgcagtatta tggtacaaac ctctgcaaag cttccgggac aggatttcag gataatgatg
     1261 attgtatgcc acaaaatccg gcttggtcac gaagttcagc agcagattct gcaatacaaa
     1321 atataatatc ccgacatact ctccttcttt cagaaaagca tcggacaact ggcctcgcac
     1381 cagatccgga tgtttcttcc gataccacca cacacccaga gggttgaatg actccatgca
     1441 gtacattccc ttgtaggccc gcagcatttt atctgcaatg gggcacaggg acaggtcggt
     1501 acgctcgatc ttgaactcca cgatcaaagg cacttttccg tctaccatct tcaatacatc
     1561 ctcaaacttc ggaatcttct ccacagaatc gcacaggtga aactgctgca attcctcgta
     1621 ggtataatca ctgatcttgc cttcgccgcc gcagactctt tttaatgtaa aatcatggaa
     1681 caccacggga atcctatcct tcgtcagctg aatatccagc tcgataccga agcctgcatc
     1741 cacggccttg cggaatgccg ccatggagtt ctccggtgct tctgtggcat tgtcatgcag
     1801 acctctatgg gcataaagcc attctttaaa aggggctgtg tccggtttgt gcagcatcct
     1861 cggcatgatc atgagaaaat acagaactgc gagaactacc aaaacaatca ataagattcc
     1921 taatactttc attcctataa ccttctatct tcctgtaact ttttacagcg aatccatacc
     1981 gtttctatca cagtatagat tcgctgcctt ttactttaat ccactactct tttattttca
     2041 agtaggtatt tcttaagatt ctccaaagag cttacaacac atttgtcaaa ttgtatcgtc
     2101 ctgccaagta tataccgaag ttatccgcat cagtcatcta cgtcaaagtt ttccagaata
     2161 ctctcttttt tctggggctt cacatcgccg tgacgtacct gggtcatagt aaggaatgcc
     2221 atcagggaga agaaaagggc atagggaaac agggtgttat aggacacatt ttccaatagg
     2281 aatccagata gtaccggagt caggatctgc gctgccatgg agaacgtata ataggttccg
     2341 gtgaatttcc ccacatcgct gccactgctc atggccacga tcatcggata ggaattcaca
     2401 ttgatggctg cccatgccac accgatcagg gcaaatgcca cattgacgat ggggtgatat
     2461 tctctggcaa aaattgcgcc accatagcag gctgccatca gcagaacacc gcccatgata
     2521 gtcttcttac gaccgatctt cgcagagatg tttccaatgg gaatatagct cagaatagca
     2581 gctaccgtcg ctaccatcag acagttggcg aagcttccgc cctccatctt ccatactacc
     2641 ttcgtatagc gggaaaaagc tgtggtcacg gcattatacg cggtgaacca taagaagata
     2701 gatgcaagca tgaagtacat actgcgcttc acttccttgg gcataccctt ggtgccttcc
     2761 gtctttcctg tctgtttttt cccgtcgaaa gattttacct ctccggtatc tcccactgcc
     2821 tcgatctcac cagcagcttc cactccctcg gccattgctt tttcttctat tgttcctgca
     2881 tttgccgcct ctgccttctc gacttcttcc cgcagcttat tttcccggat ggtcatcacg
     2941 agaattccca cagcgatcgc catgactgct gcaatacttg caaataaagg cagataatcc
     3001 ggtgtttcac ctttgcccac cagaactttg atcatgatca gagcatagac gccgcccaca
     3061 gctcccatga ggttgatcac cgcattggca cggctgcgca gatggttcgg tgtcacatcc
     3121 ggcatcaatg ccaccgccgg ggaacgatac agtcccatgg acaccagggt gaaaaacagc
     3181 accgccagga agatcacctg gttactgaag gactctcctg tgttccatct tgctttttcc
     3241 gcattgtcga tcatcggcag taacatcatg aagatcactg ccaggatcgt acctgccacg
     3301 ataaagggca ttcttctgcc aagcttcgta tgtaccttat cagacagcgc accaaacacc
     3361 ggcagcagaa agatcgccaa cacattgtcc aatgccatga ccgctcccgt catggtctcg
     3421 cccagatgat aggtattctg caggatcagg gggatgatgt tgtcatacat ctgccagaag
     3481 gaacagatgc taagaaaagc cagtccgatc agaaacgttc ttttgtaatt taattttgtc
     3541 tccataagaa aacctcccaa gttgatagtt ttattttatc agagggggat gccatgcgca
     3601 atctcaggga gtaaaatcga ggtaaatttt ctttccggaa ctgcttttgc attgggcgcc
     3661 tgtttcgaag catcagccac cacgaagtga cggaagtcgt tccagcgtgg cacactggct
     3721 ggtccaccgc ccgagaaaac gctgaaatat cacactttct ccttgaaata tgtgggcttt
     3781 tatgtataat ggaagaagtg actttgtaac gcatataaat aagaagaggt ttttagaatg
     3841 atcagtgcaa acaacgtaac ttaccgtgtg ggtaagaaag cattattcga agatgtcaac
     3901 atcaagttta ccgaaggcaa ctgttacgga ctcatcggag ccaacggtgc cggtaaatcc
     3961 acctttttga agatcctgtc cggtcagcta gacactacca aaggtgatat cgtcatcacc
     4021 cccggtcagc gtctctccgt gttggaacag gatcatttca aatacgatga caacgtggtt
     4081 atggataccg ttatcatggg taacgaacgt ctcttccaga tcatgaagga aaaggatgcc
     4141 atctatgcca aggaagattt cagtgacgag gacggtatcc gtgccagcga actggaagcg
     4201 gaattcgccg agatggacgg ttgggaagca gagtccaatg ctgccatgct gttaaacggt
     4261 cttggcatcg gcaccgagtt ccactactct ctcatgaagg atctggacgg cagcctgaag
     4321 gtgaaggtcc tgttggccaa ggcactgttc ggtaaccccg acattctgct gctcgatgag
     4381 ccgaccaacc atctggatct ggatgcgatc gcatggttgg aagatttcct gattgatttc
     4441 gagaataccg ttatcgtagt atcccatgac cgttacttcc tgaacaaggt atgtacccag
     4501 atcgccgaca tcgactacgg caagatccag ctgtatgccg gtaactacga cttctggtac
     4561 gagtccagcc agctgctgat caagcagatg aaggaagcca acaagaaaaa ggaagaaaag
     4621 atcaaggaac tgcaggagtt tatctcccgt ttctccgcaa atgcttccaa gtccaagcag
     4681 gctacttcca gaaaacgtgc actggaaaaa attgagctgg atgatattaa gccttccagc
     4741 agaaaatatc cttatattga tttccgtccc gagcgtgaga tcggtaacga ggtactggct
     4801 gtggagcatc tgtccaagac catcaacggt gaaaaggtgc tggatgacat ttccttcact
     4861 ctgggccgtg aagacaaggt ggctttcgta ggtagctgcg agctggcgaa gactactctg
     4921 ttccagatcc tggccgggga aatggagccg gacgagggaa cctacaagtg gggtgttacc
     4981 acttcccagt cctactttcc gaaggacaat accgcagagt tcgacaacga tatgaccatc
     5041 gccgactggc tgaccggtta ctctcccgtg aaggatgcca cttatgtacg tggtttcctg
     5101 ggacgtatgc tgttcgccgg tgaagacggt gtgaagaagg ttcgtattct ctccggtggt
     5161 gagaaggtgc gctgcctgct gtccaagatg atgatcagcg gtgccaactg cctgatcctg
     5221 gatgagccca cgaaccatct ggacatggag tccatcaccg cactgaacaa cggtctgatc
     5281 aagttccccg gtgtggcact gttctcctgc catgaccacc agttcgtaca gaccaccgcc
     5341 aaccgtatca tcgagatcct gccaaacggc agcctggtag acaagatcac tacctacgat
     5401 gagtatctgg caagcgatga aatggcaaga aagcgtaccg tattcactgc acagaccgag
     5461 gacgacgaga actaagacaa ataaaatgga tgccggtaat gccgacatcc attttttgaa
     5521 aggagcttta ctattatggt agatttacac gtacactcca cctgctccga tggcactttc
     5581 accccggagg aattagtcga ttacgcgatg gaaaagggac tgcgtgcctt cgccctcaca
     5641 gatcatgata ccgtaaacgg attggaccgg gcgatccggt atgcggaagg gctgcgacag
     5701 acacaggaag catccccggc tgacgctccc gcgcctcagg tgccggaagt catccccggt
     5761 atagaacttt ccacagaata ccagggaaaa gatatccaca tggtgggact tttcattgat
     5821 taccaccaac cggattttgc tcattacctg gaggatttca tccattcccg ggagaaccgc
     5881 aacaagaaaa tgtgcgcact ccttcgagaa catggcattg atatcaccta tgaagcgctt
     5941 ctggcagaat tccccggtgc agttatcacc agggcgcatt ttgcaaggta tctgctctcc
     6001 cacggctata tccagagcat gaaggaagca tttgaccgct atgtgggcga ccactgtccc
     6061 tgtttcgttc cgagagaaaa agtgaccccc gctcaggcgg tagagctgat cctcggtgcc
     6121 ggctgcgttc ccgtgctggc gcacccgatc ctctaccata tgagcgacga gcgattggat
     6181 aacctcgtgg cagaattaaa gagcatcggc ctcgtgggta tcgaagccat ctacagcacc
     6241 tacaacactg ctgaagaacg ccagatccgg gggcttgcct ccaaatacga cctgaagatc
     6301 agcggcggct ccgacttcca cggtgccaat aagcccaaga tcgacctcgg caccggctgg
     6361 ggcaagctgt atgtgccgga tgaagtgctg gaaaatctgc gcccgggccg gaaataacta
     6421 gaaagttagc atagctacga gccatgcaaa aatagagtgg aacacctcga tgggcgcttg
     6481 cgcacaagcg aggctggttc actctatttt tatacggcga cagccgtcta tgaaataaga
     6541 ttgacagcgt tccttgctga caatcttatg aatagcgtgg ctcattgcgg atggtagtt
//
LOCUS       DAWDHD010000141         6523 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_678_length_6523_cov_10.480519, whole genome shotgun sequence.
ACCESSION   DAWDHD010000141 DAWDHD010000000
VERSION     DAWDHD010000141.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 6523)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6523)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6523
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_678_length_6523_cov_10.480519"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1766 a         1743 c         1183 g         1831 t
ORIGIN      
        1 cttcctaacg ttccagtctt tcctgggttc tggccagctt tgccagtgcc ttttcttcca
       61 gttccacttc cttgacagcg gctttttcta tatcccggat atagcggtcc gcctttttgt
      121 ggttcttacg gtccaggcgg tatgggtcat acacaaagta cgataccgct cccagcacca
      181 gaatcaccac accggcaatc gtcagtaccg gtacgacgat cccatactcc gtaaatccca
      241 gttcaaaaca gacaaatacg atcagcatgg ccggaaacaa cagcagatac agaattcttg
      301 tcaccatatc caacaatgca tgctgtcttt tcccattggt cagaagtgtt ccctcaatag
      361 cggtataaaa tgccataaaa atacacaatt ccgaaccgaa gccatgaaga tatccgcaga
      421 ttccggtcag aatcagcgac agaaaaaaca tgccgaacgc cgttccctga taccaggcat
      481 tgcggtactc cgtcattccc tgcagcttgt cctcactgat gccgtgcatc cgatagacat
      541 ccttgcggat cagccgttca aattcttcgt tataccgctg ttcctctctg gtattgcgca
      601 ggtggtcttc caccgccttg ctcatctgct cgatatattc ctgctccttg cggatccgtt
      661 tggttctgag ctgctccagc tcattctggc gcctgacttc ctcttccagt ttccgtacct
      721 ttccgatccg gggattcttt accataatct ccggattctc cggaagcaca gttgtttcgt
      781 tcttctcctg attctccatt gggtcctctt tccgtttata ttatcatcca atagccttat
      841 tatagatttt ccggcccaga aaaacaacct tttgttccct gttttcattg ctttggctct
      901 ttatcctgtc actcgagaaa aatgccgcaa gtaccggcag cattaccata atggggaata
      961 aataacgtac ctgcactacc ggtcccagca acatagtccc aaagtataaa aacggcagta
     1021 agctcatctg gaagcaccag tattttcttc gatacaggca caacaccatc aaaaagaaca
     1081 ggctccagct gtatagagat ccatgaaaaa taacggaaag caccggccat tcattaaagc
     1141 agttaccact gacgatcttt tccagcactt tttccagtgc cgggaatttc gaccgatgtt
     1201 caatactgcc atagccttcg atttccgagg aagtataggt aaagagcaca ccataccttg
     1261 tgtctgtgcc atagcccaga ttttccgccc aggagaaatc atcccagaac cagtatcctc
     1321 tggtcagttc cagaaaagca tctaggaact cgttcggata ccgtttgccc actcgtagcc
     1381 agtctctcaa aaactgcctg gaattctccg tatatgccga agcatttacc gcgcccttga
     1441 tggtatctgc cagcggcgca taataagcat cccaatgatc tctcggcaca tatttttcca
     1501 gcaatgccgc cagctccgga tcctgcacct ccagttcctc tccatgataa tatgccactc
     1561 tggcaaactg ctggatggga acgctgtaag cctcgatctt cggagcctcc agccgggtcc
     1621 ctatggctgt ctggatcaca tttcgcattc cggtctctcc cagtaccaga caaatcccca
     1681 gaatcagaat acccagtttt tcctttttcg gccgcaggat caataacagc agcataaata
     1741 ccgccaccgc atatactgca ttattacgaa actgcatcat cagacagccc tcagccacca
     1801 gaagaatgtt catgaggatt ttcttttttc cgttgctgaa ataatttctc tccagaaaaa
     1861 gtaaaaagaa caacgtaaac aatatggtaa acaaggtatc ctttgtggta cacaccacta
     1921 aaaccgtgtt gtatgggaaa agcgccagaa atagtgttgt taccaccact ggccattttt
     1981 tagcagtcat ccgccagatc atggacgttg cataacccat gaccagagca tacagcacca
     2041 tctggaatac cgccatacag gccattcccg cctcgtagga tccgatggcc gttccaatct
     2101 gtaaaaacag ccggatcagc catgtatgtg caatgggctg caagggataa aaccattcca
     2161 ggcccttata cgcttcgttg acctgtctgt gaaaatcgta agacatgatg atcgggtaat
     2221 aagccagcca taccggaatc aggcacagga aaataacgac tgccgttact gcgaatacaa
     2281 tgccggactt ccaatatctc cccgacatcg ttgcttctcc tctcgcagag agtcggtcta
     2341 tcccgtcaag caacagattt gcaaaaggaa atgcggcaac acttaagcac agcgcacgga
     2401 tcaggatcat ccctttgccc cggactccgc aatccgtcat tcccagattc tgcaactgat
     2461 atcccatgac catggacaag gcaaacagga agctgatcac tgccacataa tagattctgc
     2521 gcttcctgtc ccttttctcc tctattttcc ggagattttg ctccgtctgc tgcaacatag
     2581 aatagattcc cacagccagc agcacattca gaacactgta atagatccgg ttcaccggct
     2641 gtaagctgaa cagatatgcc accccgtaga caccgcacag agtcagaacc gctctgcccg
     2701 aagttccttt gtatttcatc catttatcct ttgcaatatt caatcgtaag atgcctccat
     2761 acatttattt ccatttcttc cactatattg aaatttacac tttctgtcaa tcattgtaag
     2821 ttcttttcta tttgatatat tgtataaatt taacaaatat agtaatgatt cctattatta
     2881 tatttatgct tttcgtgcat ttcttttttt tataaaaaga gatatactat atacagaaac
     2941 aaggaaatgt ttccacaaac tcataaacca acagaaagga tgatagatta tggcaaaatt
     3001 atatacgatc actttaaatg gcgttacaga ggaaacttac aatcaggcta ccgattatat
     3061 tcagaaaaac gcactgcgtc tgaactaccg gccggttgct tccaccattg atgtggaatt
     3121 tcccgatgac atcgatcccg acaaagctcc ggaacttagc gaagccgtga tccgcgaggt
     3181 gcatcagact ctgtaatttt ttgattttta cttttgtaaa atagcaacgc aaaagtccgt
     3241 cgatgcttta acgctccgac ggactttttt gttgtgcccg ctaacttgcg gacacttttc
     3301 gcatgaagac ccttaaggtt caagtggttg ccggtattcc tcatataaga tatccatgtg
     3361 cagccagtca tagtggttcc gcatatagta acaatgcccc cggtatctgc cacttattct
     3421 gaatcctgcc ttctcgtaac acttaatggc aggggtgttc gtttccagta catccagatg
     3481 aacactatac actcccagca ccataaagca gtaccgcaag agtatctgca cgacttcggt
     3541 tccaatgcct tttccacgca ccgccttttc tccgatgtta atggataacc aggctgacat
     3601 atcctcagtt cgatggtcaa tatgacaata tccaatcaat gtgtctgtct ctttcaggac
     3661 aattcccatc cgcatgacag aatggtctct tacccaaccg ggcatccggg tgatgtccac
     3721 cacagaatgc gcatgctcta taaagacaca ggtggtttca tccgacatcc acttcgcgta
     3781 taagccgact gcctcctcat catccctcag tacggataaa aaaatttgat ttccatctaa
     3841 tctgcatata tttttactca tcatgccacc ttacatctgc tctgtgtgtt gcgaagtcct
     3901 tgatgttatt tgaattataa catagaactt cctttttggg cactatattc ttcgaacatc
     3961 cgccgcttac gcaaagccca aagtctccgc agacaatcac atagtcctgt tcgccttcac
     4021 aatctcattc aggcgtaccg gtccgtatcc atataacatg cagcccacat tataagcgtt
     4081 ctccccctga ttcatatatt cccatgcttc atccctctta tcgtgaatat gaccgtaaaa
     4141 gtgcaagtga cctctgtaag agccattcca ttccgacatc ggataatggc acagacatat
     4201 ctctttccca ctatccgaca cgtacatcat cctatcgatg gagtcacaat atcccattgc
     4261 aatctcatta tccagcagcc tggtatcatg gttgccaaga accagatgct tatgtccctt
     4321 tagttgactc agataccatt ccgcactttt ttcgtttcta tagcagaaat cccccaaaat
     4381 atacgcattg tcatcatgat ttactctttg attccataag gatatcattg cctcatccat
     4441 gacttccaca tttttaaagg ggcgatggtc aaagtcaatt actcgccctg catcttgggc
     4501 ataaagggat acccgcaggg tgttttctta attttgccct gctttctggg catagaagta
     4561 tgccgcaggc aagggcggaa aaaaggaact gccgcataga cagcccgaaa aacatggatt
     4621 ccgtgtaatt acggatttct ttatttatct ttacttccaa tccataaaca cctccttttt
     4681 agcacaaaaa aatcgccatc tttggaaaaa tgacgattct tcaagtttta ctccgtttac
     4741 ttccaactca ttcttgcgtt atatacattg tttacagctt caatatagca tatgaaagag
     4801 ggatggtcgt ggtcgttctg ccgcaggtat ctgtttttga tattcaaata ctgcgttctg
     4861 acaagctgcc attttcccct tttcttaata tacttagcct ttttctgtat tcctgcggaa
     4921 tgaggggctt tgttccaata gtattcatcc cgataagtga accctgtttt tttgtgaata
     4981 agtctgtaaa aatcttcctc tgtatctctg catacaatgc agacatccat tgaaaactcg
     5041 gtaggatttc ctctaacaaa atgaatttcc cccgtagtca gagaagatgt tgaatcctca
     5101 caatctccta aatcacgctc gtttaacgcc ttattaaagg atttcctcgc acattctttt
     5161 agatttatgc gatcctcaaa atcttcacat cttacaatct caaggttgta atccaaatct
     5221 atcgacaacg aagcattttg taccccgtcc ttaaatatat atacacgttt catccgcttt
     5281 ctcccttaca agcccatcat ttcacgcacg acacggtctg ccatcttgac cgcaccgaca
     5341 aggacgagca tattgaatac cagttccccg atatagctcc acaccatcgt gaccgcagcc
     5401 gcatcgggat tgaccacggg cggggaagcc gcaaacaccg aaaagataat gcaggcaagc
     5461 acgatgatta caccctcaag gcacacggca caataactct tgataaagct cctgccgaca
     5521 ttctggctcg gctctcccgc aaagaagaaa gagggacggg agcgattgcg gtataaaggt
     5581 acagcttgaa aaacctgccg tacaccgaca ttatcataat gaacgacagc actgtgatga
     5641 acagcccgcc gatgagcgtc accgcccata ggggaatgct ttcaaaaaag ccgcagtcct
     5701 ccacggctgt cacaatctct gtcgacaaca ccgtcttttt gcgatttgac atagatgcgt
     5761 cctcctttcg catagtttta gccactttgt caaggtggct atgtaaacaa gtacaagaga
     5821 acgcaaaaaa acgcctactc ttaaaatcaa taagaataag cggctttgta atttgcctta
     5881 gacatattca attttcattc tcttggtcgg tgcatttatg accttaaaaa taagctcgtc
     5941 aacaaaatcc gtatcttttc ctgacttaat atccttcatc atctgattaa atgaaactct
     6001 tccctcccat aatatgaata ccgaatttta atccaagttc atgtacataa gaagcaggtg
     6061 tgtaaatccg cttccgtctg ctgatgacgg aaatctgccg ggactgggct gaaaacatac
     6121 ccagtcaatc tttccacctt cacacatcat aaagaatctg gtgccattat ccaatacttc
     6181 aatacccttt tgtacatagt cagataatgc ccattctcca tctgcacgat ccatatcata
     6241 atccatagcg tcggaatctg ccagatgctc atccatcaga ataacccttc catcttcagc
     6301 cgtaaccgct tctgcatcag cctgtgtata ggtaaccttg tatcctgctt ccggtccgtc
     6361 cagtactgct cctccacggt cttcatagct attgctcgga agtgccttga cataatcttc
     6421 atctgctatt gctccaagat agtcagatgc tgcctgaaac tggggatggc tcataccatc
     6481 accaataaac agaaaacata tttcggtgct tttgctgttt ctt
//
LOCUS       DAWDHD010000142         6480 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_685_length_6480_cov_8.872529, whole genome shotgun sequence.
ACCESSION   DAWDHD010000142 DAWDHD010000000
VERSION     DAWDHD010000142.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 6480)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6480)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6480
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_685_length_6480_cov_8.872529"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1606 a         1758 c         1323 g         1793 t
ORIGIN      
        1 ccatttccgt ccaactgcga accgcatcgt atcccgaata ttatcctcca gttctttcct
       61 ctgcagcttc ttgaattcta cttcctgtct atccattccc accagctcca gcaatttgtg
      121 agagaggctt tccagacgtt ttccctgact gaaaatataa taggtggctt cgccccgctc
      181 ctgttctgtc agttcatagg agttcagcat atccgcatat ccgatgatag aagtcagcgg
      241 tgtcttgagt tcatgggcaa atgccaccgt aaaatcttcc ttctgttttg cttccagctc
      301 tttttcctcc atctgacgta ccagtctgtc cgtcatccgg ttaaagctcc tggacagttc
      361 tccgatctca tctgtgccct gatagacagc tctcttctca aaatcccctc ctgcaatcct
      421 tcccgcagtg cggtttaact gctctatggg gcgggtcaga tatctggaca gcaaaaaagc
      481 tgccagacca ccgatcacca acagacctgc cagcgcaata ccatattgtc gcatcatact
      541 actccgttct tcataaatgc cgctcagatc cttcaggatc cctagatata ccgtatctgc
      601 cgaatccgtc gcctgatctg tgatgatgct gagcagataa taacacgttc ctaccttgcg
      661 gacaccgtag acctgatctg caccgggatc catagtccgg agcatttcct ccgcaagcct
      721 cctgatctca tcctgcggca gcttcaggct gccctccaga gtgatcgtgc catctgtttc
      781 aaggatcata cattccgtac cgctctgacc ggtcatccgt tctgttgcgg aatttcctgc
      841 ctgtatccca gcaaattccg ctccgtattt ttccatgttc tgatacacca tatcatatat
      901 ggtctgaaac aaaagatttt cccgttttcc ctgttctgtc tcccgttcca gcatccggtc
      961 aaaataggaa gacagcatga aagttccaaa gagcagaaaa atcactgtaa gaagcgcaat
     1021 cactgaaaaa aacaatttat gagcgaactt cattcaaccg ataccccact ttatatactg
     1081 ccacgatcat ctcttcccag tgcagttttt tcttcagccg ctgtacatgc aggtccaccg
     1141 tgcggctctg tcccatatat tcaccgcccc atacggtctc gtatatagtc tcccggtaca
     1201 aagccctgtt tttatttctt gcaaacaata acagaagatc aaattccttc ggtgtcagat
     1261 agatttcttc tccatccagt gtcactctgt gggatgccgt atctatagtt acattgttga
     1321 cctgtatcat cgtctccgtc ttatgatatc tgcgcagcac tgcctctact ctggccagaa
     1381 gctccacgat ttcgaaaggc ttcggcagat aatcatctgc gcccatgcga agccctttta
     1441 ctttatgttc cgtagaaccc aaggctgtca gaaagatcac cggaagctca caggctttcg
     1501 cataatccat cagttcataa ccattgattc ccggcagcat gatatctaaa agtgccagat
     1561 cgaatttctc ccgctccata agatccgctg cggtctgtcc gtccggtgcc caggtgcact
     1621 ggtacccggc tctcgtcaga ttgatccgga tcagattggc aatgggctcc tcatcttctg
     1681 caattaatat tctaagcatt tccctgtccc tcgaccacat ttcataagct cacgaattcg
     1741 tgctatcgca ctctgcatcc tgcttcgcag ccgcttgttt ttcttttgca catatttata
     1801 ttatagcata tctcatcttt gtatccaatt tgtatcatct atttatttgg cactattttt
     1861 tcgtccgatc ataccatata ttaaatgaat tattggaaag gattgctatg aaagactatt
     1921 cttttgaaga acattttgaa aaatatccac tggccatggc ctatgttccc tggcagcatt
     1981 tcaccaaaat gtatgagaat ctggaaaaag gctaccaggc gggtaccatt tttccggagc
     2041 ttgacaaacc ttttaccgga aggagatgcg taaaatgaac agtgaagaaa aaatgctgca
     2101 cgacatcggc attgccgact tcgttctgac ggatctgatg ctgtatctgg acactcaccc
     2161 cacagaccgg aatgccatgg agtatttcaa ccattatacc aggatcaaga cacagatgga
     2221 gcgggaattc tccagagaat attaccccct gcggaaagat ctggcagaaa gtaacaagga
     2281 gtggcgttgg ggcaacgctc ccttaccctg ggaaggagga tgtaactgat gtggagttat
     2341 gagaaaagat tacagttccc tgtaaatatc aaggaaccca atgccaaact ggcccaggtc
     2401 atcatctccc agtacggtgg ccccgacggg gaaatgggtg ccagcatgcg ttatctgtcc
     2461 cagcgttatt ccatgccctt taaggaagtg gcaggactat tgacggatat cggaactgaa
     2521 gagcttggac atttggaaat ggttgccacc atcgttcacc agctgacccg tgacctctcc
     2581 atggaagaga tcgagaaaag cggatttgcc aattattatg tggatcacac cattggtgtc
     2641 tggcctcagg cagcaggcgg tgtgcccttc aatgcctgtg aatttcaggt aaaaggagac
     2701 atcatcacag atctgcacga aagtatggct gcggaacaaa aagcccgctc cacctacgac
     2761 aacattctgc gcctgatcaa ggatccggat gtctgtgaac ccatcaagtt cctcagagcc
     2821 cgggagatcg tccactacca gcgcttcggt gaagccttgc gcattgccca ggatcacctg
     2881 aactccagga acttctatgc gtttaacccc ggttttgacc gctgcgattc ctgcgagacc
     2941 tgatgattcc aaaaccgttt attctcaaat aacacaaaaa aacacaaatt ccgtacaaaa
     3001 aatcgagatg taggagtaat tttgtcagga atttcttaaa gtctctcttc ccgcgaaact
     3061 cattcactgt tttctatttg aaaccactgt ttttgtattt atttcgctgt tttttgtagt
     3121 ttatgtacaa tcatctgtct atctctcttc acccatgaag aacaatgcca gtgttccggg
     3181 gccagaatga gaaccaatgg tggggcccac atagttgatg aggaagctgt ccatgccaaa
     3241 acgcgccttt accaagtctc tcaccagttc cgccgcaggc agatcatcac cgtggctgat
     3301 gaaaacaata tcgttctggt cacggtagga tcccatcttc tcctccatgt aatccaccag
     3361 tgcatgcaga gatttcttac gtcctcttac cttaccgatg acaatcagat gtccatcgtt
     3421 atctacatga agcttgggtt tgataccggc cagtgttcct acaaatgccg tagccttgct
     3481 gacacgtccg ccgcggtaga ggtgatacag atcatccacg gtaaatacat gtaccagatg
     3541 cagacgatta ttcttcaccc actctgccac ttcttccatg gatttgccct gatctctcaa
     3601 agtcactgcc ttgtggacca gcagtccctc tcccagagaa gcgcacagac tgtcgatcac
     3661 gatgatcttg caatcgggct gttcctccat gatctcatcc gccgcgatcc gcatgctgtt
     3721 gtaagttccg ctcaatccgg aggagaaagc gatacagagg atctctttat tttctttcag
     3781 atattccgta aaatgagcct ttgcctcctc agggttgacc tgggatgtag tcggcatctt
     3841 accctgtctc atcaatgcat agaattcttt ttcgggaagt tcctgtcctt gtccgtaagt
     3901 cacaccatcc atgatatagc taaggttcat gcatccaaga tcatgttcct tcagatactc
     3961 taccggcaga tcagcagtac tgtctgtaac gattttaaac attggttacc ctcctgatgc
     4021 tatcaatttt tcctaagcat acatcaaaag ggcacccatt tcaattttcg ctgacaaaag
     4081 tgatacattt tgtcacattt ttctggttcc tggcattctt ccacggaaga aacgatttct
     4141 catttttgct ctgtcaaaag gaatcagcgg atacagatag ctttgtccag agattgtctt
     4201 attcgaaagg agcgccagcg caaatataat aagtccggca atataacccc atattccgaa
     4261 aagctgtgtc aaaatcagat tgaggatccg cataaatttc aaggcataac ccaattcata
     4321 attagcctgc gtgtaattag caatggccac aaatgccatg gcaagcataa cctcactgga
     4381 gaaccaccca ctctccaccg aaaattctcc cagcaccagc gccgccatga cactcaaggg
     4441 agtactcagc atattcggtg tgttgaccgc cgccagccgc agtccgtcaa tggccagctc
     4501 caacagtaaa aactgcagga tcagaggcac attgggatcc tctttcagaa taataaattc
     4561 cagattctct gggatccaca tccgatgatt cataaggagc agatatgtgg gtgtcacgaa
     4621 atacgtgagc agtgcgatca ggaaacgggt cagacgcaga tacgttccgg tgaccggcgg
     4681 aaagtaataa tcatccgcct cttccaccac atcaaagatc gtggtgggca ggatcatggc
     4741 agaaggggaa ttgtccacca gaatcaccag attgccttcc agcacctggg ctgccgccgt
     4801 atccggccgc tccgtgtatt tgaacttcgg aaacggattg taccacttcc gctggtacag
     4861 acattccgcc aggctttcct gattcatggc cagagcatcc acctgaatgg aacggatccg
     4921 ttcccggatc tgcgtaagaa gtttcggatc cacacggctc tccatataac acagtacgat
     4981 atctgttctg gaattctccc cggcattaag catttccata tgaagatccg tactacggat
     5041 cctgcggcgg atcaatgctg tattaaatac cactgtctcc acgaagccgt ccttggaccc
     5101 ccggagtacc ttgtccttgt ccggctcctc cacccctctg gtgggatagg ttctggcatc
     5161 gatcaacacc gccttttcat aaccgtccaa taacaggaca aagacgccgg acagcaaagc
     5221 tcccagtatt ttctcccagg tatcgtccag atccacctcc acgtagggaa tcgcgatttt
     5281 ggacatctca tgggcatcct ttgggaagtc ctccgccttc agatccatca gatactgtaa
     5341 tattttctgc atcagttcat ccttgcagaa accatcgatc agatacatgc aggcatcggt
     5401 gtcaccgaga cggatcaccc ggtgaataat atcgaaattg gtttccagtc ccatcagttc
     5461 atccacatac tgcatattct gctgaagctt ttccgatatt ctgacgagct ttggttgttt
     5521 gttctgtggc ataaaaaatc ccccttgggt ctatgatcta aatttcatca tgtcccattc
     5581 gggggatttt attctgctat tttgttgttt tacctgttac ttcgtggtgc ttccgaagcc
     5641 gccgttacgg attccttctg catcatcatc ttccgtgatg ccatactgca taaagattcc
     5701 ctgtgcaaat ccggttcccg caggaacctc cacggtcttg ccttcattgg aatcattggt
     5761 gatcttcatg aagatatgtc cttcattatc tgaaaagaaa tagtcactgt caatgatgcc
     5821 cacggtattg ttcatctgca aacggtattt aaagcccagt ccgcttctcg gataaatctg
     5881 taatacccag tcctccttca tttcagcacg aatgccggta ggaatcttga tggtctctcc
     5941 cggggccagg gtaaaggcaa acggtgcgaa gaaatcatag cctgcgcttc ctttcgtggc
     6001 tctcctggga agttttaaat tttcatacat ttccttgatc tgtgcttccg tatactgagg
     6061 atatccctcc tgcatggcag tcaaaaactg ccccggactt acaaaatgaa attttgctat
     6121 tctgtgcatt gtctgttctt tcctttttct tcttacatta ccggctgaaa ccagccgctt
     6181 cctatgcgta atccctgcag tgcaataccg gcacccgtct atggtcactg gccagggtct
     6241 tcttcacatc gatcacccgc tggttcttac tgcccttcca caggagcttt acatcttttt
     6301 cctccacatg gaattcaccg tctactacca catccacata ctgcatcacc ggcagatcac
     6361 agatgtcttc ccagctgtcc ccggtgtaca tccagatcgt tgtcgtggga taattttccc
     6421 ggagttcact catgaggtct atgccatccg cccgctacac gggatgtctc gtaaacacat
//
LOCUS       DAWDHD010000143         6444 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_692_length_6444_cov_7.604789, whole genome shotgun sequence.
ACCESSION   DAWDHD010000143 DAWDHD010000000
VERSION     DAWDHD010000143.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 6444)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6444)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6444
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_692_length_6444_cov_7.604789"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1783 a         1465 c         1636 g         1560 t
ORIGIN      
        1 cgctggcccc tgatctgttg tcaccgtaac aacttgtggt taagacctat ctggtaaagc
       61 cggtaaagca gctgtccgca gaatatatcc accggttgga aaacgggctt gacatcggtg
      121 atgagaaacc gacagccccg tccaaagcca gactcacaga aaacggtgat ctgttattga
      181 ccattcatga aggccggttt catcaggtaa aacggatgtt gcaggcggtt gataatcagg
      241 tacttacact ggagcgggta agcttcggcc ccctgtccct ggatttttcc ctgcaaagag
      301 gggaataccg ggcattgacg gaagaagaga tcgaagtctt gtctggctct ggaaagaaat
      361 cataaataaa ggagttattt ttcatgcaat tcaaaagaat gtttaccact gatgcctata
      421 aaggaacccg cgggtatctc cgcacacaga aaacttatga gattttaaga accgtgcttt
      481 attttgccat ctctctgtct ttgtttatcg ccggatgggt cagcaccgga agccgtgaga
      541 atctcctgac catcgtggcg gtactgggat gtcttccggc ctgcaagagt ctggtagaaa
      601 tgttcatgtt cctgcgctgc cggggctgtg acgagcaggt agctgcaaaa atcgcggcac
      661 atacagaagg ccttaccact ctctacgata tggtatttac ttcctatgag aagaattatg
      721 ttatctatca tctgacagtt tgcggtaata cggtctgtgg ctacaccacg gatccgaaat
      781 ttgcggaaca ggctttttac aagcacattc aggacattct gaaaaaagac aattacagag
      841 aagtctctgt gaaaatattt aacgatctgg acaagtatct gacaagatgt gatcagttaa
      901 aggagctttc ctcacagccg gaactgtctg ccgggatctg ccagacctta aagagcgtat
      961 ctctataact tacttctcaa aaggagggtt tcctatgcag ttgcttacca atggcaaaaa
     1021 tcaggcggga caacgtctgg acaaattttt aaaaaaagcg ttacctaatg caggcaccgg
     1081 atttttatat aagatgcttc gcaagaaaaa tattacgttg aatgggaaaa aggctgaagg
     1141 taaagaaatc cttgcccagg gagacgaggt aaaatgcttt ttctctgagg aaacttatgc
     1201 ctctctgtcc ggtgccgggg cagcggaaga tacttccgat taccgtaagg cttaccgttc
     1261 cttaaaggat atcagcgttc tttatgagga tgaaaatatc ctgttgctga ataaacctgc
     1321 cggagttctg acccagaaag caaaaccgga agatctttcc ctgaacgaat ggctgatcgg
     1381 ccacctgctg gctgccgatg ccatcaaaga aaccgatctt gccaccttcc atccatcggt
     1441 ctgcaaccgc ctcgaccgca ataccagcgg tatcgtattg tgcggaaaga cgctggccgg
     1501 ttcccaggca ctcagccgga tcatcaagga tcgttccgtg aagaaatatt atcagaccgt
     1561 ctgtaagggc aagattttac aggaaagcac cctggaaggc tatctgtaca aggacgaacg
     1621 caccaatacg gtacaggtat ttccagatat ggcggaggct ccggaagatg cttcctttat
     1681 aaaaacaatc tacactccca gtgcggtggc tggggaatat accctgctga ccgtagaact
     1741 ggtcaccggc aaaacccacc agatccgtgc ccatctggcc ggcgccggac atccgttact
     1801 tggtgacacc aagtacggag acagcaaatt aaatcgcagg atgcaatcgg aatactctct
     1861 gcaccaccag cttctgcatg ccggaagagt gtgtttcccg gaagaagcgg aaggacctct
     1921 tgcaaaggtg agctgtcaga cttttgacgc accgcttccc cataaatttg cagagatctt
     1981 acaggctctg aatctgacac cggattccgc ctgctagaaa ggaaactaag aacaatggca
     2041 acatggaata ccagagggct tcgtggatct actctggagg atatggtcaa ccgcaccaat
     2101 gaaaaatatg ccgaggccgg tctggccctg attcagaaga tccccacccc aattaccccg
     2161 gtcaaaatgg ataaggagag ccgccagatc accctggcct ttttcgaaca gaagagtacc
     2221 gtagactata tcggtgtcgt acagggcatt ccggtctgct ttgatgccaa ggaatgtgcg
     2281 gtggacacct tttccctgca aaatattcat cctcatcagg tggagttcat gcatcagttc
     2341 gagaaacagg gggggattgc ctttttcctg atcttttaca gccataagga tctgttatat
     2401 taccttccct atgaaatgct ccgctttttc tgggacaggg cacaggaggg cggccggaaa
     2461 agcttccgtt tcgaggaact gaacccggaa tatattatcc cgaagcatca gggaatcctg
     2521 gttccctatc tggatattct gaaaaaggat ctggaagacc gggaggaata ataaataata
     2581 atttcttgac agcggagagg tctcctagta tactacaaat agtttcacat gctactatga
     2641 taatatggta tcacgttaat acattaaagt gtatgaattg aggaacgctt atgaacaaca
     2701 gacttttaca tacgccggag ggcgtccggg acatttacgg caaagaatat gcaaagaagc
     2761 tggctgtaga acagaaactg cagcaggcga tccattgttt cggttacgaa gatattcaga
     2821 ccccttccat tgagtttttc gatgttttct ccaaggagat cggaaccact cccagtaagg
     2881 agctgtataa gttctttgat aaagagggaa atacgctggt gctgcggccg gactttacgc
     2941 cgtccatcgc ccgctgcgca gcgaagtatt ttatggagga aaaggtgccg cttcgtttca
     3001 gttacaacgg caatacgttt acaaacactt ccaatctgca gggtaaatta aaagaggtga
     3061 cccagatggg tgcagagctg attggggatt cttccgtgga agcagatgcc gaagtgatca
     3121 gtatggtgat tgaagcactg aaaagtgccg ggctgaccag atttcaggtg accattggtg
     3181 aagtagatta cttccgcggt ctctgtgaag aagccggtct ggatgaggat acggaaaagg
     3241 aactccgttc ctgtatctcc ggcaagaact ttttcgctgc ccaggaatta ctggagcaga
     3301 agcagatccc cgagccttat cacagcattc tgttaaagct ggcggatatg ttcgaaaata
     3361 tagagtctct ggaagatgcg aaagcactgg ttaaaaatga gagatccctg caggcgattg
     3421 caagactgga aaagttaaaa gagcgtttag agcagtacgg cgttgctgaa tatatttcat
     3481 tcgaccttgg aatgctcagc aaataccagt attataccgg cgttgtcttc aaagcttaca
     3541 cttacggcat cggcgatgcc gtggtgaagg gcggccgtta tgacaacctg cttcggaagt
     3601 tcggcaagaa tgcggcagct atcggttttg tcattgttat tgatgatttg ttagaggcat
     3661 tatcccgcca aaaagtgaat atcgatattc ctgattccgg caaagtagtg tattatcggg
     3721 caggagacga ggcagattat ctggcgaagc tttccgaagt cgtaagttta cgtaagcaag
     3781 gcattcccac tgtgctttcc cccgagatta cagagaacga ggaggcagat cagtaatgag
     3841 tgaagaaaga tatcttacgt ttgctctggg aaagggcaga cttgccaaaa agacactgga
     3901 gcttttcgaa cagatcggca ttacctgtga agaaatgaag gataaagata ccagaaagct
     3961 gatcttcgtc aatgaagaat acaaattacg ctttttcctg gcaaagggtc ccgacgttcc
     4021 cacctatgta gagtacggtg ctgcggatat cggtgtggtc ggcaaggaca ccattctgga
     4081 agagaaccgg aatgtttacg aagtgctgga ccttggcttc ggcaaatgcc ggatgtgtgt
     4141 ctgtggtcct gcttccgcca gagagctgtt gaaacaccat gaacggatcc gggtagccag
     4201 caaatacccc aatatcgccc gagagtattt ctataataaa aagcatcaga ccgtagatat
     4261 catcaaatta aacggttctg tagaattagg gccgctggta gagctgtctg acgtcatcgt
     4321 ggatatcgtt gagaccggtt ccaccttaag ggaaaacggg ctggaggttc tggaagaggt
     4381 atgtcctctg tccgcccgta tgatcgtcaa tccggtgagt atgcagatgg aagccgaccg
     4441 tatccgcaaa ctggtgggtg cgatccggga gcagttaaaa atacaggcct gaccgagacg
     4501 ataagcatcg tcataaaaag tatttaaagg agagtaccgt catgagaatc gtagcattgg
     4561 ataaagaatc aaagcagagt attttgaacg accttttaaa gagaagtccg aacaattatt
     4621 cccagtatga gagcactgta aaccagatca tcgaaaaagt ccgtgccgag ggtgacaagg
     4681 cattgttcga ttatacctta cagtttgata agttcgcttt aagcgcagag aatatccgtg
     4741 tcacaaagga tgagatcgcc gaagcctacg cacagatgga tgagaatctg atcgatgtga
     4801 tcagacagtc tgcggaaaac atccgcgctt ttcaccagaa gcagcttcgt aacagctggt
     4861 tcgatgccaa gcccgacggt acgatcttag gcatgaagat caccgccatc gaaagagccg
     4921 gtgtctatgt ccccggcggt aaagccgctt acccttccag cgtactgatg aatgtgatcc
     4981 ccgctaaggt agccggtgtg gatgagatca ttatgaccac ccctcccgga aaagacggta
     5041 aggtcaatcc cggaactctg gtagccgctg atattgcagg cgtggatacc atctataagg
     5101 tcggcggtgc ccaggctatc gcagccatgg ctttcggaac ccagtcggta cctaaggtcg
     5161 acaagatcac cggtcccggt aacattttcg tagctcttgc caaaaaagct gtttatggct
     5221 atgtcagcat tgattccatt gcgggcccca gcgagatcct ggtactggca gatgagaccg
     5281 ctacccccag atacgttgca gcagatctgc tgtctcaggc agagcatgac gaactggcaa
     5341 gtgccattct gattaccact tcgaaggaac tggcggagaa agtctccgag gaagtggacg
     5401 gcttcgtaaa agtactggag cgtgctccca ttatccagaa gtctctggac aattacggct
     5461 acattctgtt agccgacaac atggaagatg ctattgatgc cgccaacgcc attgcttccg
     5521 agcatctgga gatcattaca aagaatcctt ttgataccat gacccgtatc cgcaatgccg
     5581 gtgcgatttt cctgggagag tatgcttccg aacccttggg tgattatttt gcaggtccga
     5641 accacattct gcccaccaac ggtaccgcac ggttcttctc cccggtaaac gtggacgact
     5701 tcatcaagaa gaccagtatt atctcttatt ccaggcaggc actggaaaag gtgcacagcc
     5761 agatcgagac cttcgccaag agtgaaggct tgaccgcaca tgcaaacagt attaaagtta
     5821 gatttgaaga ttaatcattt cctctgggaa gggagtcatc ggatattatg gaacgaattg
     5881 caactgtgac ccgtacaacg aaagagaccg acattgccat caccttaaat ctggacggca
     5941 ccggaaaagc ggatctgtct accggcatcg gattttttga ccatatgctg gacggctttg
     6001 cgaggcacgg cttttttgac ctggaatgca aggtgaaggg cgatctgcag gtggacggtc
     6061 accacacggt ggaggatacc ggcattgtct tcggtgaagc catcaaaaaa gcggtaggag
     6121 acaagaaggg catcaatcgt tacggctatt ttattctgcc tatggatgag gtacttgctt
     6181 tgtgcgccgt tgacttaagc ggccggcctt atcttaattt tgaatgtgaa tttactgtac
     6241 cgagggtagg tgaactggat acggagctgg taaaggaatt tttctatgcc gtatcctaca
     6301 gtgccggaat gaacttacat atcaaaatgt tgtctccggg caataatcac catatgatcg
     6361 aagccatgtt caaggcattt gccaaggcgc tggatcaggc cgtttcctat aatccgaagg
     6421 taacggatgt tttaagtacc aaag
//
LOCUS       DAWDHD010000144         6440 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_693_length_6440_cov_11.755834, whole genome shotgun sequence.
ACCESSION   DAWDHD010000144 DAWDHD010000000
VERSION     DAWDHD010000144.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 6440)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6440)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6440
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_693_length_6440_cov_11.755834"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5464..5563
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1686 a         1413 c         1662 g         1579 t
ORIGIN      
        1 tattaccggc tatggagtga tcaatggtaa tcttgtatat gtatatagcc aggacgcttc
       61 cgtactgaac ggaaccatcg gcgagatgca tgcaaagaag atcaccaatc tctacgatct
      121 ggctatgaag accggagcac ctgtgatcgg actgatcgaa tctgcaggtt taagactgca
      181 ggaagcaacc gatgcactgg ctgcatttgg tgagatctat ttaaagcaga ccatggcttc
      241 cggcgtgatc cctcagatca ctgctgtatt cggaacctgt ggcggtggct taggactgtt
      301 ccctaccatg accgatttca ctttcatgga agagaagaat gccaagctgt tcgtaaatgc
      361 tcctaacgct ctggacggta atgtgatcac caagtgtgat tcttcttccg ctaaattcca
      421 ggcagaagag agcggtattg ttgacgtagt agctgacgaa gcaaccattc tggagaaggt
      481 aagagagctg gtaagcttcc tgcctgccaa caacgaggat gatgcaagct tcttagagga
      541 ttgcacggat gatctgaacc gtgtaaatcc tgagatcgca ggttgcgtcg gcgacacatc
      601 cgttgctctg tccattctgg cagatgacaa caacttcttc gaggtgaagg ccggatatgc
      661 taagaacatg gtaaccggtt tcttacgtct ggatggcgtt accgttggtg cggtagctaa
      721 ccgttccgag atctgcgacg aagaaggcaa ggtagcagag aagctggatg cagttctgac
      781 tgcagagggc tgtgagaagg ctgctgagtt cgtaaatttc tgtgatgctt tcggcattcc
      841 tgtactgacc ctgaccaatg taaagggtta cgaagcaact ctggcttccg agaaggctat
      901 cgcaaaggct gctgcaaagc tgacctacgc atttgccaac gctaccgttc ccaaggtgaa
      961 cgttgtgatc ggcaaggctc tgggaaccgc ttatgtggta atgaactcca aggcaatcgg
     1021 tgcggatatc accatggcat ggccggatgc acagattggt gctatggacg gcaagctggc
     1081 tgccaagatc atgtatgatg gccagggcgc tgatgtgatt aatgaaaagg ctgctgagta
     1141 cgaagcactt actctgaatg taaccagcgc agccaagaga ggatatgtgg accagatcgt
     1201 aaatgctgct gataccagaa aatatgtgat cggcgcattc gagatgctat tcaccaagag
     1261 tgaagaccgt cctgccaaga aacacggaac tgtataagaa agggtgattg taaaatgaag
     1321 aaatttttaa gcttagtatg tatgattacc tgcatctttg gtttgacggc gtgcggcagt
     1381 gaggagacct atacggatta tgagcagcgc aagatggaca ctgcaatcca gatagctacc
     1441 cagtatgtga ttccttctct ggagaatttt gaagacgaag ctgcactgga gagcttctct
     1501 gagtataccg ctgatgaagt ggcatacatg gtacaggaaa atgtaggtat caccgttgac
     1561 ggttatgcat acaagactgc tatcgagtcc ttcaattccg cgaagaaaac catcggcggt
     1621 atcactgctg tcggtgatgc agatgctagc atcgatgatg atcagatcat tgtacatgta
     1681 gatgtgaccg gtgccaaaca gaatgcacag gcagaagtga tcttcaccaa cgatatgttc
     1741 ctgagcatgg agtctgcagc gctgaacccc gtggagtcca tgggtggtct gatgaccaaa
     1801 gctgcactga acactctgat cggtatggga actgtattcg taatgctgat catgatctca
     1861 ctgatcattt ccctgttcaa cttcattccg aagatccagg ctgcattcag caagaaggat
     1921 aaggaggagg aagcaaagaa cgcaggtatt gataaggctg taactcagat cgctgcacag
     1981 gaagagcccg aggcagatga cactgagctg gtcgctgtta ttgcagctgc gatcgcagca
     2041 agcgaaggtg cggcaagcac tgacggattt gtagttcgtt ctatccgcaa agtaagataa
     2101 tcgtaaaaac gttaggagga tattaaaatg aaaaattata ccattaccgt aaatggcaac
     2161 gtatatgatg tagtagtaga agagggcgca tctaccggag cacccgtagc agcaaaggct
     2221 cccgcagctc ccaaggcagc acccaaggct cccgcagcag ctcccaaggc aagcggcgca
     2281 gcaggcagca tcaaggttga ggccggtgca gctggtaaaa tcttcaaggt agaggcaagt
     2341 gttggacagg ctgttaagaa gggtgatgct gtcatcattc tggaagctat gaagatggag
     2401 atccctgtag tagctcccga agacggcact gtagcaagca ttgacgttgc agttggtgat
     2461 actgttgagg ctggtgcagt tctggcaact ctgaactagg cattttacat cagctctata
     2521 aacaacagga ggtcatcaaa tggaatatat agctagtacg cttaacaacc tgattcatca
     2581 gacagcgttt tttaacctga cctggggaaa ctatgtcatg attctggttg catgcttttt
     2641 cctttattta gctatccgtc atgagttcga gcctttgctc ttactcccga tagcgttcgg
     2701 tatgctgttg gtaaacattt atcccgatat catgctgcac cctgaaaatg cagcaaacgg
     2761 tgccggcggc ttactgtatt atttctacaa gctggatgaa ctggcaatcc tgccttccct
     2821 gatcttcatg ggtgtaggtg ccatgacaga ctttggtcct ctgattgcga accccaagag
     2881 cttccttctg ggagctgctg cgcagtttgg tatcttcgca gcatacttcg gagcaatctg
     2941 gctgggattc aacgacaagg cagctgctgc aatctccatc atcggtggtg ctgacggccc
     3001 tacctccatc ttccttgctg gtaagctggg acagacggcg atcctgggac ctatcgcagt
     3061 ggcagcatat tcctacatgt cactggttcc cattatccag ccccctatta tgaagcttct
     3121 cactactgag aaagagagaa agatcaagat gggtcagctt cgtcccgtat ccaaactgga
     3181 gaagatcctg ttccccatcg ttgttaccat cgttgtatgt ctgatccttc ccaccacagc
     3241 tcctctggta ggtatgctga tgctgggtaa cctgttccgt gagtccggtg ttgttcgtca
     3301 gctgactgag acagcatcca atgctctgat gtacatcgta gttattctgc tgggtacttc
     3361 cgtaggtgct accaccagcg cagaagcatt cctgaatgca gataccttga agatcgtagc
     3421 tcttggtctg atcgcattca tgttcggtac cgccgcaggt gttctgtttg gtaaactgat
     3481 gtgtgtattt tccggtggta agatcaatcc tttgatcggt tccgcaggtg tatccgcagt
     3541 tcctatggca gcacgtgtat cccagaaggt tggtgcagaa tccgatccta ccaacttcct
     3601 gctcatgcat gctatgggtc ctaacgtagc aggcgttatc ggtactgccg tagcagccgg
     3661 tacattcatg gctatcttcg gtgtatttta aagcgaatgc ccaagatgta tttcgcgcaa
     3721 agcgcgatga aataagtccg aaggacttag aataacgcat cgcgtcaagt ctaattatag
     3781 aaaatattat tatggaggat attcgtaatg tcagaaattg aaaagaaacc gattaagatt
     3841 acggaaactg ttttacgtga tgcacatcag tctctgatcg ctaccagaat gccgaccgaa
     3901 ttcatgcttc ctatcgtaga taagatggat aaagttggtt atcattccgt agagtgctgg
     3961 ggcggtgcta cctttgatgc atcccttcgt ttcctgaagg aagatccctg ggacagactg
     4021 agaaagctgc gtgacggctt caaaaacacc aagttacaga tgctgttccg tggacagaat
     4081 attctgggtt acagacctta tgcagatgac gtggtagatt acttcgtaca gaagtccatc
     4141 tccaatggta ttgatatcat tcgtattttc gactgtctga acgacctgcg caacttacag
     4201 gaagcggtaa atgctaccaa caagttcaag gcacaggaag gcagaggtga agcacaggtt
     4261 gctctggctt acactctggg tgatgcttac actctggagt actggaccag catggccaag
     4321 aagatcgaag agatgggtgc agattccatc tgtatcaagg atatggccgg tctgttagta
     4381 ccttacaagg ctgctgaact gatcaaggcg atgaaagaag ttaccaagct tcccatccag
     4441 ttgcatactc actatacttc cggtgttgca agcatgactt acctgaaggc agtagaggcc
     4501 ggtgttgatg tgatcgatac cgctatgagc cccttcgcta tgggtacttc acagcctgct
     4561 accgaggtta tggttgagac cttcaaggga accccttacg ataccggtct ggatcagaac
     4621 ctgttagctg agatcgctga ctacttcaga ccttacagag atgagtgcct ggctaacggc
     4681 ctgctgaatc ccaaggttat gggtgttgat atcaagactc tgctgtatca ggtacctggc
     4741 ggtatgcttt ccaacatggt tagccagctg aaggagcaga atgcagaaga cagatactac
     4801 gatgtgctga aggagatccc caaggttcgt aaggatctgg gtgagccccc tctggttacc
     4861 ccttcttctc agatcgttgg tactcaggca gtcttcaacg tactgcttgg tgagcgttac
     4921 aagatggcta ccaaggagac caaggcagtt cttcgtggtg agtacggtca gaccgttaag
     4981 cccatgagcc aggaagttat cgacaaggtt atccccggtg aggagagaat tacctgccgt
     5041 tacgctgatc tgctcgacaa tgagatggat aagctggaag gcgaaatgaa ggaatggaaa
     5101 cagcaggatg aggacgttct gagctacgct ctgttccctc aggttgctac cgacttcttc
     5161 aagtatcgtc aggcacagca ggaaaaggtt gatatgaccc aggctgacac caagaacggt
     5221 gcataccctg tctaaataat tcgtcaataa aaagtcgcac gactgctcgc agtcagtgcg
     5281 gcttttttat tagacgaaca aaaccacatg agcaagtagg gcgatagccc ggattgcgaa
     5341 tgtggttgcg gcttcgagag cgcgaagcgc ggagaagccg ttatttagac atgtccccgg
     5401 tcgaaccggg gcgatagccc ggattgcgaa tgtggttgcg gcttcgagag cgcgaagcgc
     5461 ggannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngaagccg ttatttagac
     5581 atgcccttgg tcagccccca gtcagcccga tatgcgaatg agtttattta catattgaca
     5641 aaccacctct tcacccacta aaataacaag tgttatagaa ggacacattg tttgctgtaa
     5701 atacagcgga atgagaggga ttatggctcg taaagagatg atcacgattg ataaaatcct
     5761 ggatacggca tttgccatga caagggaaga gggatttgcc aatgtgacag ccagaagggt
     5821 agcggcgaaa gcagggtgct ctacacagcc tattttcaga gtatataaga atatggaaga
     5881 actgtgggat gcggtctatg agagagctgc agcttatttt ctggattatt acagcttgta
     5941 tccccgtacc ggcaagacac ctttcgccaa tctgggcatg gcttacatag cttttgctag
     6001 agaagagaga cacctgttcg agcttctgtt tgtatcggat aaccaggatg gcaaacataa
     6061 gaaaaagagc atgtatgaga tcttgaatgg tgatgccgga aacgtggtct acgagatcaa
     6121 cctggccaga gtggcgggct gcccggatcc cggtgatctg ttcatgaaaa tgtggatttt
     6181 cattcacgga gctgcctgca tgtctctgac cggagattat gatctgacag acttacagac
     6241 aatgcagcta ttagagcatt cctattacgc tttttacaat gagacaacgc gaggttaaga
     6301 accgtggcag aatatgatgt ttttacccga atcaacgaac attatgcaaa aatgagcaaa
     6361 ggccacaaag ccattgcgga ttacatatcg gaacattacg atcaggcaga aaatatgacc
     6421 gcaggaaagc tgtgtgagac
//
LOCUS       DAWDHD010000145         6367 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_703_length_6367_cov_11.186312, whole genome shotgun sequence.
ACCESSION   DAWDHD010000145 DAWDHD010000000
VERSION     DAWDHD010000145.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 6367)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6367)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6367
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_703_length_6367_cov_11.186312"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1514 a         1718 c         1267 g         1868 t
ORIGIN      
        1 acgtttacaa gtgtatattt agccataaaa aaccgacctc ctatcgttag atttaaggtc
       61 taacgatagg aggtcggttc aggataaggc tatttttcaa tccatatcaa aaattatcca
      121 ttttgaacaa ttcacttctt gtttttattc agtttcgttt tcttcctcta atagctttaa
      181 taatgcagaa ggttcacaca ccttaacaga agatttcata taatctttta catttctggt
      241 tactatacaa tccatttctg aacgaattgc tgtttccacc ataatagcat cttcataatc
      301 accaatttca gaagatattg ctttccggca atctaaagaa gtggtatcca acaaatgaaa
      361 taaagtaaaa agcttgctca aaatctttcg tgtttctacg tcgttatgtg ttaaacggtg
      421 tgtcagataa tagatatctg taactgactt tgctgtaata taaccctcaa attgcttatt
      481 tgcagaatat ataaagagtt tttgcgctgc ttctgaaaat ggagcacgag actgtaatgc
      541 atcgatgata acacaagtat caactaaaac tctcatatcg tattcaacct ttcttcccgt
      601 gcttcttcca gagtcatatc atttgaaaga acgccaaaca atgatttagc aacatctact
      661 ctatcctgat atggattaga caattttgca acaacttttc cgttacgagt aatataaata
      721 tcctctgttt ctgcaagcat aagatatttg ccaagattaa tttttaattc agttgcagta
      781 atggacatat catcatcacc tttcttgaag aataactatt tatcgtctca taaagagaat
      841 ctctcttttc aattatatta tacacaaatc gaacgatttt atcaataaaa tcgttcgaaa
      901 atcgactatt gaatgctggt cggagcgcta ctccgccaga tacatcttca ggcttccttc
      961 caccgcatcc atagcttcct ccacactcgc ctgcccgttt aggtatgcct gtaagctctc
     1021 ggtcagggaa gtctcgatct gagtgtcttc cttaaggcac agggtggctg ccttgcacag
     1081 ctccatcaga tggttcgcgg tctcctcaga gtaggctttg atggcgaact ctgcggtact
     1141 gccgtcgata tcataggtgg tataggcttc cgccatgctg cggtctgctg cagcctgcgt
     1201 ctccaacgct tttgcattga cagggaatcc ctcataggta tccacgctct gcaattcttc
     1261 ggacagagca aaccgcagga aatcctttgc gatctccacg tgttcactcc ggctgttgat
     1321 tccaatgacc atcttcggat aaaatgcatt ttccacggat accacattgg catttaacag
     1381 ttctgccaca gcatagggtg tcattgcctg attaaagccg tcagtctcct gaagtaccag
     1441 cttcacatca gatcccagat cccagatatt cgctgcacgg ccttcttttc tggaaggcag
     1501 gataccgcag ttatccgcaa tcttcttcaa atcttccagc cagggacgta aagaatcacg
     1561 attcacctga cggttctgta aaatatcatc gacgatcagg ggatagaact cttccaccag
     1621 ttcgccgcag gtacggtctc ccatataact ctccgtcttt ccggataccg cctttgcaat
     1681 agcatccatg ctgctcattt cctccggctg cacatccctg ccgacagcta acaataatcc
     1741 gaattgcagc ggcaccgcat accgtgttcc atccgcctct ttatacgctg ttgtaatgtt
     1801 ggaaagcagg gttccgtcct cttccatggg ctgcagcacc tcctgaagat ccaacagcaa
     1861 gcccttagag gcatatgtgt ccagtttcag atgatccatc accatgagat ccggtccgtt
     1921 gccggaggtc agggaagtgt taagatcctg atatatctgc tgataatccg catttccgct
     1981 gtatttatcc gtcatggaga ttgccgcatc cacatggatc agtacctccg gatgctgttt
     2041 atgatactgc accgcagcct gctgtaaaaa gaagctctcc tccacggtat acagcgtcag
     2101 ttcttcggtc acctctgtca ccgcatcggg atcatatcga taaatcatta ttttatcccc
     2161 gctctcactt ccaaaccacg cgtatacact gccgtccgac agcgccacaa tatccctgca
     2221 ccactgatcc gacagagaaa aacctgtatc gattccctga agcagcttct gccagttggt
     2281 atcgcctgcg tcgcagcgga agaatccgtc tgcatccgcc gcatagactg tgccgtctcc
     2341 cagcacactg aaataagcag agctgtaact gtcctgggaa aaaggaagca cttccttctt
     2401 ttttgcccca ttgcttccat ccagctgcat ctgcaacaga ccgttcacgt tcccctgttc
     2461 atccgtctgt gccagataca acgtatctcc cacagcactg acccacttat tatccgacag
     2521 actgccactg tctgtcaggc tgtcgctctc ctggctgctc accaggcttc cgtcctgtgc
     2581 caatacaaca tccagggaaa aataagacag tcccactaac aattcatcct ctgtcagtgt
     2641 cacatactgc ggcgtatcat agaaccgata gccatccata tccataggct ccagccactt
     2701 ctgtggtgta atatccaacg cactgccgtc cgcttccttc cacagatgtg cggaagagct
     2761 ctcctcatcc cggtaacagt tgccgtacag gtactgattc ccctccgcat cctgcaacag
     2821 tgtaaaactg tcactgctct ccagaccctt tccctcggga agcgccttca gccagtcctt
     2881 cgtcacttcc gtcagagtat ccccttcgcc cagcatccat tcctctaaag agacctgacc
     2941 ttctgcgcct cctgccacaa gaaaatgtaa ttcatcacct gaacggaaaa tctggctgat
     3001 atttttgtcc ttccattcct ccggaagtga gagttccgtc tctacatatc gtcctttctg
     3061 cacttcatca ggtgacgctg cgatctgctg atcgttctta ttcccacacc cggtgaacag
     3121 tcccatgacg attgccatac aggtcgccag tgcgattcct ttttgcatgt tattgttact
     3181 gttattccaa taaattctgt tttgcatctt aatccctatg cctttctccc attctgctgt
     3241 gtgttccaca gctttttctt acaggaaaag tatagcggtt tcttttttaa gataacagca
     3301 ggcacgggtt aagatgcctt taccatatga ttaagatttt cttaacgcaa aacggaaccg
     3361 gcacaagcgc cgattccgtc ttctccggaa acccttttag gaattcaata tcttcataaa
     3421 ttcttctccg gtaatggtct ctttttcata taaatactgg gacagttcat ccagtttagc
     3481 acgattttcc aacaggatct gccctgcctt ctcatgctgc ttcttcacca gctccaccac
     3541 ttccttatcg atggcagtct gggtctccat ggaacaggtc agggaagcat ccccacccag
     3601 atactggttc gtcacggtct ccatggctac catgtcaaaa tccttgctca taccgtagcg
     3661 agtgatcatg cctctggcaa gcttggttgc ctgctcgata tcgttggaag ctcctgtggt
     3721 cacagaaccg aaagcaatct cttccgcggc acgtccaccg gtcaatgtag cgatcttgtt
     3781 ctccatctct tctttgctca tcagataatg attgccttcc tcttccacct gcatggtata
     3841 acccagtgca ccggaggtac ggggcacaat agtgatcttc tgtaccggtg cggaattggt
     3901 ctgcttcgct gccaccagtg cgtgtccgat ctcatggtat gccacgatcc gcttttcctt
     3961 atctgtaagg atggcattct ttttctgata accggcaatg actacttcaa tactctcttc
     4021 taagtctgcc tgggttgcga acttacgtcc atctctgact gcacgaagag ctgcctcatt
     4081 gacgatattg gcaagctccg caccggaggc tccggaagcc atgcgagcaa tcttgttgaa
     4141 gtccacattg tctgcgactt tgatcttacg ggcatgcact ttcaggattt cttctcttcc
     4201 tttcaggtcg ggaagctcta ccggcacacg tctgtcaaaa cgtccgggac gtaacagtgc
     4261 gggatccaga ctctccggac ggttggtggc cgccaggatg ataacaccgt tgttgccttc
     4321 gaatccatcc atctcggtca acagctggtt caatgtctgt tctctctcat cgtttccgcc
     4381 cacctgtccg tcacgctttt taccgatggc atcgatctca tcgataaata cgatacaggg
     4441 agccttttcc ttcgcctgtt tgaacaggtc tctgaccttg gacgcaccca tacccacgaa
     4501 catctccacg aattccgaac cggacatgga gaaaaaggga acattagctt ctccggctac
     4561 tgccttggct aacatcgtct ttccggttcc gggagggcct actaagagga tacccttcgg
     4621 catggaagca ccgatctctt tgtatttgtc gggatcatgc aggtattcca cgatttctgt
     4681 cagattttcc ttcgcctcgt cctctcctgc cacatcggtg aacttgatac cgttggaaga
     4741 cttcacatag accttcgcat tgctcttgcc catgcctccc atgccgaaca tcatggaatc
     4801 gccgccggcc tttttcgcca tctggcggta catcaattgt ccgaagccaa taatgatgat
     4861 caaaggcagc agccagctga tcaggaagct taaaaccggc gatgcttcct ccacgatctc
     4921 actggagaat tttgccccgc tgtcatacag tctctgcgtt ctgtccggat catccatcag
     4981 accggtcttg tagatggtct tgtcatcttt tgccgtgaat acgatctgat tgtcctggac
     5041 ctgtacctca tcgatctgtt tatcataagt catctgcata aaagtgttgt aatctacttc
     5101 cttcactgcc cgctgtacga tctgcggtac cagaagactg ttgatcagga taatcacgat
     5161 cagtgcaatc atataataga atatgaacgg ttttttgttt ggttgtttga cttcctgcat
     5221 acattttccc tttctcattc ctgtattatt gtccaaatct gttgtaataa atcaatcata
     5281 gccacttttt tccacacaat caatggatat tctcttacct tattctaaaa aaaagataaa
     5341 cttatgcggt gaaaagacta cgtttttcaa aatctgtttc tttccatttt caggcaaaag
     5401 agagacaatc aaaatagatt tttacttgcc aaaaggttca aaaaggagga aacgtctgtg
     5461 tcggatacta ccaaaaagag attaaaattc gcaggaaagc ttctgtttgt ctgtctgtta
     5521 ttaggtatcc tgtattacag cttccgggat tccatgggag atatgctgac ggagcttgcc
     5581 aatgtctccc cgcaagtggt catgtccctg ttcctgaccg tgatcctcta ccatatcttt
     5641 gaagggcaca tcacctggct gatcgtgcat aaaacgcacc cggaatatcc ccgcctgaag
     5701 ggattctgta atgctttcta ctgctctttc tataagacag ccactctggg cagcggttcc
     5761 ggcatcgcag gaatctatta cctgaacaaa gccggtatcc cggttcccaa tgccaccggc
     5821 atgtactttt tccaatatat tgtccacaag gtctctatgg cagtctatag tctgctattg
     5881 tttttggcaa cctatggatt tattcatgct tcatttgccg attatcaatg ttatatcctg
     5941 ctgggatttg caggagtctg tgtgattgca ggagttctgt tagctgtcgc taccgtaccc
     6001 tggatgcaga ctgcctgtaa tctgctggcc aaccggttcc gtgcaaagca tccctcctgg
     6061 gaggaaaaac tcaccggtgc cgggcataag cttgccctgc tacaggcgga aagccggtct
     6121 ctgttacagg atccaatatt gctgcttcac ctgttcctgt gtaatgtatt aaagttcact
     6181 atctggtata tcatcccctg gatcgtgttc tgcaattctc ttgggggaga atacggcgat
     6241 ctgccttttt ctttcctgca atgttttgct ctgacctctc tcagccagtc gctggcgggt
     6301 gtcatcccca ccccggcggg gatcggatcc gtagaagctg tctttacatt gctgttccgg
     6361 cgtctcc
//
LOCUS       DAWDHD010000146         6257 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_724_length_6257_cov_13.463883, whole genome shotgun sequence.
ACCESSION   DAWDHD010000146 DAWDHD010000000
VERSION     DAWDHD010000146.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 6257)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6257)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6257
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_724_length_6257_cov_13.463883"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1797 a         1277 c         1656 g         1527 t
ORIGIN      
        1 aggaagtcaa catgtttaca tgattgacga acgacaacct tagcgaatga atatcctgtg
       61 aagtaagatg tagagtacga tggtacgaat gcgtgagctt ataatagaat tacataagct
      121 atatcgagga agaacagaaa ataatacata aaacagtgga ggacaggtta tgaccaatac
      181 aacgaaactg gcgttggaag cagctttgaa aaaagaactt ctcatgaagt ctctggataa
      241 gatcaccatt aacgaacttg cagaggactg cggcatcagc cgcatggctt tttactatca
      301 tttcaaggac atctatgatc tggtagagtg ggtctgtgtg gaggacggca cgaaggcact
      361 gcaggggaag aagacctacg agaactggca agaaggcttc tgccagattt tcgaggcagt
      421 gctggagaac aaacctttca ttatgaatat ctaccgctgc gtggatcgcc agaagatcga
      481 aagctactta aacaagctga cctatcagct cattgcggac gtggtggagg aaaaatgtgc
      541 cggcatgcag gtagcggagg aacacaagga attcattgcc ggattttaca aatacggttt
      601 tgtgggtgtg atgttagact ggatcgaccg gggaatgaag gacgattatc ataagatcgt
      661 tgaagaactg gcaatcactc tttacggaaa catcagtcgc tccatcgaaa attttgagca
      721 gacagaaaag aaagtctgaa atagtataag tactttacaa aaagggcagg atgataaaaa
      781 ctttatcatt tgtccttttt tgtaaattgg ttttcttata aaaaagaagt aggatactat
      841 ttgtaaacag aaaaacattt gagagtcatc aaagaaaggt ggtaatcatt atggcaaaaa
      901 agaataatat cggactggga ctggcagcgc tggcagtagg tgcaggcgca gtggcagcag
      961 taaccaaggg acataaggca gaggtgaaga aagaggagag aaaggcagct gtcaaggcag
     1021 ccaagaaaga ctaccgcaat accgagcttg gaagatacga caagaacagt aagggcatct
     1081 attattcaaa cggtaactac gaagccttcg caagacctaa gaagcccgca ggagtggacg
     1141 agaagaacgc ttacatcgta ggcagcggtc tggcatcttt agcagcagcg tgcttcctgg
     1201 tacgtgacgg acagatgccc ggttcccata ttcacatctt agaggcaatg gatatcgcag
     1261 gcggtgcctg tgacggtatt tttgatcctg ccagaggcta cgtcatgcgt ggcggacgtg
     1321 agatggagaa ccattttgaa tgtctgtggg atctattccg cagcattccc tctctggaag
     1381 tacccggagc cagcgtgctg gatgagtatt actggctgaa caaggaagat cccaactatt
     1441 ctctgtgccg tgcaacgaag gattgtggac aggatgccca taccgatggc aaattcaatc
     1501 taagccagaa gggctgcatg gagattatga agctgttcat gacaaaggac gaggatctgt
     1561 atgacaagac cattgaagat gttttcgatg atgaagtatt taactccacc ttctggttat
     1621 attggagaac catgttcgct ttcgagaact ggcacagcgc actggaaatg aagctttact
     1681 tccaaagatt tatccatcat atttccggac tgcctgactt cagtgccctg aagttcacca
     1741 agtacaacca gtacgagtct ctgatccttc ccatgaagaa gtatctggag gcagcaggtg
     1801 tggacttcca gttcaacacc gaagtgacaa atgttatttt tgatatcaat gacggcaaaa
     1861 aggtggcaaa ggctatcgag tgcaaggtaa aaggtgtaga gaagggcatt gtcctcaccg
     1921 agaacgacct ggtattcgtt accaacggaa gctgtaccga gggtactatc tacggtgacc
     1981 agaaccatgc acccaacggt gacgcggagg ttcgcaccag cggcgtatgg agcctgtgga
     2041 aaaatatcgc aaaacaggat ccttccttcg gacatcccga gaagttctgt tctgatgtct
     2101 ccaagactaa ttgggaatcc gctaccgtga ccacactgga tgacaagatc attccttata
     2161 tcaccaatat ctgcaagcgt gaccccagaa gcggtaaggt cgtaaccggc ggtatcgtaa
     2221 gctgccagga ttccaagtgg ctcatgagct ggacaatcaa ccgtcaggga cagttcaagg
     2281 aacaggacaa aaatcaggta tgtgtatggg tatacggtct gtttaccgac gtacccggcg
     2341 attatattaa gaagcccatg aaggattgta ccggtaaaga gatcaccgaa gagtggctgt
     2401 atcacttagg tgtgcccgta gaagatatcg aggatctggc agagcacagc gcagtctgcg
     2461 tacctaccat gatgccttat attaccgcat tcttcatgcc cagaaccaag ggtgaccgtc
     2521 ccgatgtcat tcccgacggc tgtgtgaact tcgctttctt aggccagttt gcagagaccc
     2581 cgagagacac cgtgtttacc acagagtatt ccgtgagaac cgctatggag gctgtctacg
     2641 gactgttagg tgtagacaga ggtgtgcctg aggtatgggg aagtgtatat gatgtcagag
     2701 agttattgga cagcagcgtg aagctgatgg atggcatgtc tcctctggag attgaactgc
     2761 ccggaccttt aaatgcactg aagaagccgc tgcttcgcgt agtgaagggc actgtagtgg
     2821 aaaaactgct gcgtgaccac aatgtgatca aagacggcat gctggattag aattgtatat
     2881 ggaataaaag tgcgaagact cccaccggaa aaatccggtg ggagtcttcg cactgcggcg
     2941 gttatgaaat atgtcgcaaa agcgacccgc cgcaggcgga gaatctcgca agcgatattc
     3001 ttttttatgc aaaaagcttc tgtaaaaagc atgtaaagaa agttgaacaa atgtaagcgg
     3061 ttacataata ccaaagtact agacaaaaca aacaaaaaca caatagctat tttcgacatt
     3121 atgcacgaaa attggtagtc tgtcaaaaaa ttatcaattt cggcttgata ttctattggt
     3181 agattgttac aatatcaaag tactaaaaac cgaggccgaa atgctccggt aatggtttgg
     3241 gttacaaagt tttgtcaaaa atattataaa aagggagaca aagaagtatg aagaaatggg
     3301 tatatttgtt taccgaaggt aatgcaaaca tgcgcgaact gcttggtggt aagggtgcga
     3361 acctggctga gatgaccaat attggtctgc cggttcctca gggcttcacc attacaacag
     3421 aggcatgtac acagtattat gaagatggac gcgagatcaa tgaagagatc cagggtcaga
     3481 tcaacgagta cattactaag atggaagaga tcaccggcaa aaagttcggc gaccacgaga
     3541 atcctttgtt agtatccgtt cgttccggcg cgagagcatc catgcccggt atgatggata
     3601 ccattctgaa ccttggtctg aatgagaccg ttgttaacgt aatcgctgag aagtccggca
     3661 accctcgttg ggcttgggac tgctatagaa gatttatcca gatgtattcc gacgttgtta
     3721 tggaagtagg taagaagtac ttcgagcagc tgatcgatgc aatgaaagag aagaagggtg
     3781 ttaagcagga cgttgagctt actgctgatg atctgaagga gctggctggc cagttcaagg
     3841 ctgagtataa ggctaagatc ggtgtagact tccctgatga tcccaaggaa cagctgatgg
     3901 gcgctatcaa agctgtattc cgttcctggg acaacccgcg tgcaaacgtt tatcgtcgtg
     3961 acaacgatat cccttattcc tggggtactg ctgttaacgt tcagtctatg gcattcggta
     4021 acatgggtga tgactgtggt accggtgttg catttaccag agatccggct accggtgcta
     4081 agggcctgtt cggtgagttc ctgaccaacg cacagggtga ggacgtagta gcaggtgttc
     4141 gtacacctat gaagatcgct gagatggctg acaaattccc tgaggcattt gctcagttcc
     4201 aggaagtatg taagactctg gaagatcatt acagagatat gcaggatatg gagttcactg
     4261 ttgagcatgg taagttgtac atgttacaga ccagaaatgg taagagaacc gctcaggcag
     4321 ctctgaagat cgcttgtgat ttggtagatg agggcatgag aaccgaggaa gaggcagttg
     4381 caatgatcga tcctcgtaac ctggatactc tgttacatcc tcagttcgat accgctgctc
     4441 tgaaggctgc aactcctatc ggcaagggtc ttggtgcttc tcccggagct gcatgtggta
     4501 agatcgtatt caccgcagaa gatgcagaag agtggcatgc aagaggagag agagttgttc
     4561 tggttcgtct ggagacctct cctgaagata tcaccggtat gaaggcttct cagggtatcc
     4621 tgaccgttcg tggtggtatg acctctcacg cagcagtagt tgctcgtggt atgggtacct
     4681 gctgtgtatc cggttgtggc gatatcgcta tggatgagga gaacaagcag ttcactctgg
     4741 ctggcaagac cttccatgaa ggtgactggc tgtccatcga tggtactacc ggtaacatct
     4801 atgatggcga gatcaagact gttgatgcta ccatcgccgg tgagttcggc cgtgtaatgg
     4861 cttgggctga caaatatcgt aagttaaaag ttcgtaccaa tgcagatact cctgcagacg
     4921 ctaagaaggc tcgtgagctg ggcgctgaag gaatcggtct gtgccgtact gagcatatgt
     4981 tcttcgaaga ggacagaatc gcagcattcc gtgagatgat ctgttccgac actgtagagg
     5041 agagagaagc agcactggag aagattctgc cttatcagca gaacgacttc aagcagctgt
     5101 atgaggctct ggagggcaac cctgttacca tccgtttcct ggatccgcct ctgcatgagt
     5161 tcgttcctac cgaggaagct gacattgaga agctggctaa ggcacagggc aagagcgtag
     5221 agaccatcaa gaccatcatc gcttctctgc atgagttcaa ccctatgatg ggtcacagag
     5281 gatgccgtct ggctgttact tatcctgaaa ttgccaagat gcagaccaag gctgttatcc
     5341 gtgcggcaat cgaggttaag aaggcacatc ccgattggaa tgtagagccc gagatcatga
     5401 tccctctggt atgtgaagtg aaggagctta agttcgttaa gaaggtagtt gtcgagacag
     5461 ctgatgctga gattgcagct gccggtgtag atatgaagta cgaagtaggt accatgatcg
     5521 agatccccag agcagctctg actgctgatg agatcgctaa ggaagctgat ttcttctgct
     5581 tcggtaccaa cgacctgacc cagatgacct tcggcttctc ccgtgatgat gcaggtaagt
     5641 tcctgaacgc ttactatgat accaagatct tcgagaacga tccgttcgct aagctggatc
     5701 agaccggtgt cggcaagctg atggagatgg ctatcaagct tggtaagcct gtaaatccta
     5761 agctgcacgt tggtatctgt ggtgagcacg gtggagatcc ttcttccgta gagttctgcc
     5821 acaagattgg tctggattat gtatcctgct cacctttccg tgtacctatc gcaagactgg
     5881 ctgcagcaca ggctgctatc gccgataagt aatttggcgg taaacacgca gatctgagag
     5941 ggtattgcgc aagcaataaa ttggggtata tgagaataag atgagaacct cattactgat
     6001 agaaatatca gtaatgaggc tcttttttgt tactatttcg tctgaattaa agcacgatat
     6061 ttccgtcccg gtcgattgat gtcgtttttg ggtcgattgg tgcaatctgc cgcttatttt
     6121 ctggaaatgc tgttataaaa aatgtaaaaa cgtaaaaaac gtaaaaactc aattgaaaaa
     6181 gtaacttcca gttactaagg cctttatatt ttataaaaca gaaataagtc atacaaacat
     6241 gaagatggaa gatagtc
//
LOCUS       DAWDHD010000147         6233 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_726_length_6233_cov_13.084170, whole genome shotgun sequence.
ACCESSION   DAWDHD010000147 DAWDHD010000000
VERSION     DAWDHD010000147.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 6233)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6233)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6233
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_726_length_6233_cov_13.084170"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1629 a         1406 c         1891 g         1307 t
ORIGIN      
        1 cgaagtgacg gccatcgagg gagccatcat agccagcgta gtgccagatg tgatgcattc
       61 cctgaccact tccatcatcc gttatctcaa gacaaaacca ctgatcgtgg gccccggtgt
      121 gaagagcggc atcaaggtcg ctaccgagga tccccgggcc atcggtgcgg accgtatcgt
      181 ggatgcggtg gcagcttatg tgaaatacgg cggaccggtg ctggtactgg atttcggtac
      241 ggcgaccacc tatgatctga tcacggagga cggacgtttt actgcaggaa tcactgctcc
      301 cggtatccgg atcagttcgg aggctctgtg gaaagagacg gcgaagctgc cgaatattga
      361 gatccgtaag ccgaagacca tcctggcaca ggagaccatt accagtatgc aggcaggact
      421 catgtacggt cagatcggcc agacggaata cattatccgc aaggtaaagg aagagagcgg
      481 gcttgcggag ctgaaggtcg tggccaccgg aggcttaggc cgtgccattg cggacgagac
      541 cgacagcatt gatatctatg acagttctct gacactggat ggactgcgga tcatctacga
      601 caagaacaga cgttgatagg ggaatgacat gataaagctg gaaaaagcag caacaaagat
      661 tatggcagcc atcggaacag cagtaatgct gttcctgctg ttttattcct ggaaatatac
      721 gaagaggctg gtggaaccgg aatattatct ggatgaaaaa gacagtatcc tctggaatgc
      781 tcttgtcggc gtagtgctgg tggcggtatg cggggctctg tccggagtga tgaaaaagat
      841 cccggcgtgg gcactgcatc tgctggcagt tctgggagct gtgacagcag tgattctggg
      901 gataaggctt gcgagggccg ccggagcgga gagcattgcg gaccagtatt atgtgcaaag
      961 agcagctatg gcactggcgg acggtgatgc ggaatgggtg ctggcacagg attactatgg
     1021 tatctatccg ttccagctgg gactggcgga gatcttcgcc ttaatcttcc gtgtggcagg
     1081 ctccacggaa cggctcgtga tccaacaggt acaggcggtc tgcggaggca tcacgctgta
     1141 tgtgggattt aggatcgtgc gggaactgtc gggaagccgg gcagcggaac tgctgtatct
     1201 tgtggcggag cttttgttcc tgccggtgta ttgctatgct ttgtttatct atggtgagag
     1261 tatcggaacc tgcagcgtcc tgtgtgccat ctggtgttac ctgatggcca accggccgga
     1321 gggaaaggtg tggcagaagg gactgctgtg gagtgccgtc gcgttgttca tgggactggc
     1381 gtatgcgacc agaaatgtat tgctggtggt atggatcgcc atggtcatca tgcagatcct
     1441 ggtgctgctt cgcagtaaaa aatggcaggg gctgccggct ctctgcgtgg tgctggcagt
     1501 catgctgctg gggaaaacga ctctctgtca catggcggaa ggacagcttg accgggaata
     1561 tggttccggt gctccgtatg ttctgtgggt tgccatggga atgcaggaga atcccgacgc
     1621 cctgaagggg ccgggaacct acaatgacta taatctggac acttatctgc aggccgggta
     1681 tgacagcata gaggcagcag aagtggccaa aggagatatc aggcagtgtc tggcaaaatg
     1741 gcaggaggat cccgtggcag ccgttatgtt tctcaaggag aaactgctga tccagtggat
     1801 cgagcctact tattgcagct ttaaccagac cagataccag taccggccgc aaggctgggt
     1861 ggaagacctc tataccggac aggccaatga aagagcactg gcattcctga accgttttca
     1921 gggaatggta tatctggcgg ttttcggata ttatttgagg atcctgctgg gaaaactgaa
     1981 aggtgtacag gtacttccgg ggattatttt tctgggcggc tttttcatta cgatcctctg
     2041 ggaggccaag agccgttatg tgtatcccta tatcgtcatg atccttccca gcgcggcatg
     2101 cagtatggag tattacggcc gtttgctggc gggtggcatc gggaggatcg caggcggtat
     2161 tgcgagtagt agggaaagga aacagaaaca aaaagaaata ggaaggacag catgaaaaaa
     2221 ttacagattg gtaatgtgac cctggacaac ccggtgatcc tggcacccat ggcgggagta
     2281 tccgacctgc cgttccgcct gctttgcagg gagatgggag cggcgctggt gtgcatggaa
     2341 atggtcagtg ccaaagccat atattataac aataaaaata cggacagtct catggagatc
     2401 catcccgagg aagtgccggc ttccctgcag ctgttcggct cagatcccca gatccttgcg
     2461 gagatggcaa aacggatcga ggaacgtcct ttttccatac tggatctgaa tatgggctgc
     2521 ccggtgccga aggtcgtgaa caacggcgaa ggctccgcgc tgatgaagga tccgaagctg
     2581 gtggagcaga ttctgaccgc actggtaaag gccatcaaca agccggtgac agtgaagatc
     2641 cgaaaaggct tcgacgatga ccatgtcaat gccgtggaga tcgcgaagat cgcagaagcc
     2701 tgtggtgtgg cagcggtggc ggtgcacgga cggacgagag cccagtacta cagcggacag
     2761 gcagactggg atatcatcgc acaggtgaag gatgcagtga agattccagt gatcggcaac
     2821 ggggatgtgg acagcccgga aaaagcaaaa tccattctgg aacagaccgg ctgcgatggt
     2881 gtcatgatcg gccgggcggc agaaggaaat ccctggattt tccgggaggt ggtatcctat
     2941 ctggaaaacg gcacgatccc ggcaagaccc acgaaccggg agaagcggga aatgatcctg
     3001 cgccatgcgg cattgcagct ggaatacaag ggagaataca ccggtgtccg ggagatgcgc
     3061 aagcacctgt cctggtatac ggtgggaatg ccccattccg ctcatttccg ccagaccatc
     3121 aataccatgg agaaaatgga cgaactgatc cggggagtgc aggaaatctt cccggacgaa
     3181 gtgtagtgcg gattttggat acagcaatgc attatgaggc agaccttgca tgatatgatg
     3241 ttgtgcgcgg tgtggtgcat ataattggga tgactgcata caatgaagca tggacactat
     3301 tttgtatctc tatccggcgc gacggacagc gatgtgcggg acaccgccgg aggaaaaagc
     3361 ggaactgcgc cggggaaaac tgaagaaatt tttgcccttc ctgtggaaaa agggagaaga
     3421 ccggcgccct gtggtgactg tgagccggac ggaatatttt ttcccggact atcatctggt
     3481 cagagtgtcg gtagccagaa cggaaggtat ggaagaaaaa caggcggaac tggcactcac
     3541 ggagctaaaa ctccgggaac aattacagga actgacagag gatccggatc acacctatgt
     3601 ggtgtgccgt gagcctcttt ctttttattt cggaagggaa ttccgggatt atctgcaaca
     3661 ggagtggatc gaacatctgc tgcgctacgg ggagagagcg ggcggctatc cggcgtggcc
     3721 ggatctggtc ctgctgggga gaaatccgta tctgccttat gtcgtgctcc gctatgctgc
     3781 cagattgcag agcatgaaat ggtatcttac ggaaagagaa taccgggagg aggaagagtg
     3841 gctgaccggg gaactggagg atgagtacgg tctgatcccg gagatccggc tgcttgcgga
     3901 aaatgcggac tacggacgag tacggctgca ggggacgaaa ccctgcgtgg tactggattt
     3961 ttgcgacaat gaaaaggtct acggcggcgg actggcagca ggcagtatct ggctggattt
     4021 tccggcatcg gaggacaagg agcaccgggc cctggtacaa tgtccgcaga tccgttattt
     4081 ttccgtgaaa aaagagtgga aagacccgcg aaaagccttg gaatatcttg acaccatcag
     4141 taaaaatggg tataataact cggttgatta ggctagtata gttgccggga gtgtcggtag
     4201 cgcaaaatat agaaaacagg aaggtgcggt atcatgcagg aaaagaaaaa cattttgacc
     4261 tacgaaggcc tcagaaaata tgaggatgag ttacatgatc tgaaagtggt aaaacgtcag
     4321 gaagttgctc agaagatcaa ggaagcaaga gagcagggag acctgtccga gaacgctgag
     4381 tacgatgcag cgaaggatga gcagagagac atcgaagccc gtatcgaaga actggagaag
     4441 atcctgaaga acgcagaagt cgtagtcgag gatgaagtag atctggataa gatcaacatc
     4501 ggctgccagg tcaagatcct ggatattgaa tacagcgaag agctggatta caagatcgta
     4561 ggttccaccg aggcgaacag cttaaagggc aagatctcca acgagagccc cgtaggtaag
     4621 gcactgatcg gccataaggt cggcgatacc gttgaagtag agacacccgc aggtgtattc
     4681 tcatataaga ttctggagat ccagagaagt aacgttgagt aagtaagaca gagtaaggag
     4741 tggaaaaaag tggcagatac acagaacaca aaggtacagg aacaggacat tcaccagctg
     4801 ttgaaggtaa agcgcgagaa gctggcgaac ctgcaggaag caggcaagga tccttttcag
     4861 atcaccaaat atgatgtgac ccatcacagc accgatatca agaacaattt cgaggaactg
     4921 gaaggcaaga ccgtccgcat cgccggacgt gtgatgtcca aacgtgtcat gggtaaggca
     4981 tccttctgta atgtacagga tctgcccggc aatatccagg tatatgtggc tagagacagc
     5041 atcggtgaag agtcctatgc ggatttcaag aagtacgatg tgggcgatat cgtaggtatc
     5101 gaaggtgaag tcttcaagac caagactggc gagacttcca tacatgcggc aaaggtaaca
     5161 ctgctgtcca agagcttaca gatcctgcct gagaagttcc acggcctgac caataccgat
     5221 atccgttatc gtcagcgtta taccgacctg atcatgaatc aggacgttaa ggatactttc
     5281 gtaaagcgtt ctaagatcat cagcgccatc cgccgttatc tggacggcca gggcttcctg
     5341 gaggtagaga cccccatgct ggtatccaat gccggcggtg cggcagcaag acctttcgag
     5401 acccactaca atgcactgga tgaggacgtg aagctgcgta tctccctgga gctgtacctg
     5461 aagagactga tcgtcggcgg catggagcgt gtcttcgaga tcggccgtgt attccgtaac
     5521 gaaggtctgg acaccagaca caatccggaa ttcacactga tggagctgta ccaggcatac
     5581 accgactatt acggaatgat ggatctgact gagaacatgt tccgctatgt ggcacaggaa
     5641 gtatgcggta ccaccgtgat cccttacgca gaagagacca tcgacctggg caagcctttc
     5701 gagagactga ccatggtaga tgctgtcaag aaatacgcag gcgttgactt cgaccagatt
     5761 cccgataccg ctgctgcgaa gaagctggca gacgagaagg gtgtacacta cgaagagcgt
     5821 catgccaagg gcgatatcct gaacctgttc ttcgaagagt tcgtagaaga gcatctgatc
     5881 cagcccgtat tcattatgga ccatccggta gagatctctc ctctgaccaa gagaaagccg
     5941 gacaagcctg attatgtaga gcgtttcgag ctgttcatct acggacgtga gatgtgtaac
     6001 gcttattccg aattaaatga tcccatcgac cagagagagc gtttcaaggc gcaggaagct
     6061 gcactggcag ccggcgatga ggaagccaac accaccgatg aagatttcat gaacgcactg
     6121 gagatcggta tgcctcctac cggcggtatc ggttacggta tcgaccgtct ggtaatgctg
     6181 ctgaccaact ctcctgcgat ccgtgatgtt ctgctcttcc cgacaatgaa gtc
//
LOCUS       DAWDHD010000148         6218 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_730_length_6218_cov_12.160474, whole genome shotgun sequence.
ACCESSION   DAWDHD010000148 DAWDHD010000000
VERSION     DAWDHD010000148.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 6218)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6218)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6218
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_730_length_6218_cov_12.160474"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1578 a         1759 c         1329 g         1552 t
ORIGIN      
        1 ctctcctttt gtccagtgta gcagttcccc tatggaaaag caagtccatc tgtgctacac
       61 tcgttttaca ggtttttagc atccggcact gttttatctc caaagtttgc tgtagaatag
      121 cggaacccgt ggtagctgat ggactttgcg gataacttat gccgggctgc cgccgtgaat
      181 gaaaaacaat aattcagaaa ggatacttta catgaataca agaaagaacg caccgttggc
      241 tcttgtcacc ggtgcctccc gcggcatcgg taaggccatc gcagaagccc tggccgaagc
      301 cggctaccat ctgatcctca cctgtagcca taccttgccg gaccttcagg aagtggctgc
      361 ctgtttagaa gaaaaatatg gcatcacctg caccgccatg gctgcggacg catcggaacc
      421 cgctgccatc aaaaaagttt ttgcctccct ggaccatctg gatgtactgg tcaacaacgc
      481 cggaatctct tacattggtc tgctcacgga tatgagtgtc gaagaatggc agcatgtcct
      541 gaatacgaac ctgagctcct gcttctacac cagccgtctg gcagtgcctc tgatgctgct
      601 gaagcacagc ggacgcatta tcaatatttc ctccgtctgg ggaaatgtgg gagcttccat
      661 ggaagtggct tactccgcct ccaagggtgg tgtgaacagc ttcaccaaag cactggccaa
      721 ggagctggct cccagtaata tccaggtcaa cgccatctcc tgcggtgtta ttgatactgc
      781 catgaaccac tgcttctccc ccgaggaaat gaatgccctc cgggaagaga ttcccgccga
      841 ccgtctgggc cagccggagg aagtcgcaaa acttgtcctc cagctcatcc aggcgccaac
      901 ctatctcacc ggacagatcc ttactcttga cggaggctgg caataacact ctccgccgat
      961 tctcccggta ccagtgatgc cacgatccgc tccatctctt ctttggatcc agcatcccgg
     1021 caccggagga tcaggtgttc cttctccgtc tctgtcagtg ccgcatagcc ttgcatggcc
     1081 tgttcattca tcgccagcgc catgccgaat cctaccggca gttcattcaa gggaaacgct
     1141 tctgttccgc cgcttccccc agtcaatcca tccattgcaa actcctttcc cgcgttctgc
     1201 gaagatgtct cattttacag gcatcctgtt tttgcataac aatcgtgcac ttgatcgata
     1261 acataatttt gcatatgtta tcgatcaagt gctactgttt agtctgtgca ggaggaatct
     1321 gcgatataca aaacgaaaac tcgctctttg taaatgaaaa tctcacgaat aacggtattc
     1381 tccgccatcc gtgagatttt cagtttgctt tctgtctgtc aggtatccaa cattattttc
     1441 cggcaagata ttctctcata tgttctctga tatgctcgaa ctgttttctc gtatgagaat
     1501 ttttcgactc aaaattcatc agcttatcca gatacccctg tctcttcaca atacggcagc
     1561 tctcgggata gaccagctcg tacaccagcg aaatatgtcc caccacgttg tccactgctg
     1621 ttttcttcag ggatcgtagt acggcatgct cctcatcaaa gctctgcagt acttccggcg
     1681 tcacttcctc ctggtataat acctcggtgt ccacattgta gatctcttcc agagggaatt
     1741 ccacattgac cttcaagata tcaatcttat ccgcatcccg taagatatgg cagaacattg
     1801 ccgtacgttc gtccagtccc tccggaatcc ggtaggcgct gtgataaaac accgccgtcc
     1861 acagaagctc atcgcaggaa gcatcttcca cgaaatcccg gatactgatt ccggcgtctg
     1921 cctgaatttc ctcggacagg ctttcttcgc ttccggaagc aagtccgtgt ttctcttccc
     1981 agaccttacc aaataaaatc cttgctccgt atttcgcatg atcgatggaa tctgcatcgg
     2041 tgaaggtacc atatacccgc tgttgttcaa agcgtcccac atcatggagc agtccggtca
     2101 gccacgcaat gtccacctct tcctccggaa ggtccagaga tctggcgatc tgctgacaca
     2161 atccgcagac ccggtaggtg tgttctattt tcagacgaat catgtcccgg ctgctgtcat
     2221 aacggtccgt gtattcctga aatgctgcca gtgctttttc tctgttgatc ttcattgttc
     2281 gttgcctctt ttgcctctta tctcttatat catgtttcat gactcatgac ctgttcggtt
     2341 ttttcaaatc cattccttct tatctgatgg tttttgtctc tgtcaggcct ttttctctgt
     2401 cgacgataca taggccagct tgtataccgc tacacagatc actgcaacca ccatcagcaa
     2461 tcccagctcc aatgcccatg taggaatcgt ggaggcacac accacagtca gaaagtccac
     2521 tgtaaaatgc agtccgaagg ctgctgccaa atgcaacttt ctgcctgtct tcaccgcact
     2581 gtatacaaga atcgacaacg cgatctgcag ggcgatcgca ctgatccgct ccaccggtgc
     2641 gaggtagaac tggtaagccg gcgtggtcca gagaacagag agctgatggt aggttacctc
     2701 ctgttgtgcg ggctccaatg ccgtcatagt cttctccatc agaccaccgt tgatcatggc
     2761 tgcggtaagg agattgctca tagaagacat accaaggatc agaaaagcct caatgccgcc
     2821 atgtccggcg ccgtacatca atgcattttc ccgggtaaga tgttttttca ggcagaattt
     2881 catggcgatc caccggccac actcctcaaa aagtgctgct gcaagcccac cgtaaatcgc
     2941 atacagccag atattgtccc ggatcacgct tccggtcacc gtcagcacga tggcatggca
     3001 gatctgctct aagatcatgg caaagcccac gaagataccg cagccaatga aaaatgccgg
     3061 cacccacgcc tttgtcttct tatagataaa gatacctaaa aatatgggta aacccacgga
     3121 taatagtaga gatatcacca ttcccaacat gcttgcaccg cttacggttc cccagatcat
     3181 tgttgtttcc actgcatttg ttactgtcat atactctcct atctgagcgc cacgggacgc
     3241 tctgcgcacc tgttatttca ccgttccctc ttgtaagatt ctccggaaaa aggtaagaaa
     3301 accttcctct accgccacct gatccaccgc cgtctcaggc agatcataga aatatttccc
     3361 ggttccggga tccagggaaa tgctgtccag cggaaacccc tctctgcgga tgctgctgtg
     3421 gggtacatcc gtcagccaga atttctcact ttccatatca ggctccatcg cctcatatac
     3481 atgttcctca tcctgcacat agcagatggc attgcggtat tttgccgtaa gccttccata
     3541 gcgtttgacc agtccgatat agtgctccag catctgctca tccgtcagat acacaccgtt
     3601 caccgtacgc acatgtaccc cgggctggat cgcctccggg acatcttcaa aatgcaaccc
     3661 ggaatcacag gaaaatacag gcatacgaaa agcttcataa taagcatgag ctttctgcct
     3721 ggcattctcc agaggtgtat tgcctgtctc cgggacatcg gggatctcca ccccctgtgc
     3781 ctgcagatcc tgcagactga tcagttcaat gccaagtccg gctatgcggt tctgcatggc
     3841 agctgccttt gccggattgc cggtaccgta taacagtttc ataagcgttg tcctgcttcc
     3901 tttccggtca gatcagcgcc ggtgtctgtg ccaggacaga gataatcgcg atcgccgcca
     3961 aaatcacaac aactaccact actgcaacaa tgatcttctt tttcatatat gatacctgct
     4021 ctttctaact cttttatgct tttgcatccc atggactaat gatggcatat ttcaaggaca
     4081 atttcaagga ttatgaaaat attcgagatg gctccacttt ctcgcccggc caggattttc
     4141 tgagcgccgc ataggaaacc aggaaattgg caagccagcc ggccggcacg gcaagccaga
     4201 caaagtcaat gccaaaatgc ggcgcacaga tcaaagccac ggacaggcgg atcgcaaggt
     4261 ttaccatgtt ggcaataagg aatgggcgca tgattccaag tccgcgaaga actccatcgg
     4321 tcgccatttt aattcccata aagataaaga agtaaccaag ccatctcata taatctccgg
     4381 acacctgata agccagcgcg gttccgtctt ttccaagaaa tagcgaagaa atctgcgtgt
     4441 gcagtgtttc aatgacaaga aaggcaacgg ccgcaaaaca cacgtccaac accaatgccg
     4501 catgataacc ttttttgatg cgttcggttc tgcctgcccc aaggttctgg gaaacatacg
     4561 gcgaaactgc attaccgatg gacacgaaga tcagggaaaa tacgttctcc acccgcatcg
     4621 tcgccgcata tcctgcaaga gcctgtgtgc cgaaaggatt taccactgcc tgtacgaaca
     4681 tcattccgat cgacccggta gactgctgca aaaccgaggg cactgcaatc cgaagcatcg
     4741 agcgcagctt acgcccgtca aaccactgaa acgggctttg gtatcgccgc atccggtata
     4801 agaaaatcaa aagtgaaaaa acagcggaaa ttccctgggc aatcagagtt gcaagcgccg
     4861 caccgaatac accaagcccc agaccggcta ccatccaaag atccatcaca atattcagga
     4921 tggatgaaaa gatcagcagc cccagcggaa tttttgattc accgatggaa gtgaacatgg
     4981 tggagagaat attgtacatg aacaaaaacg gaaagcccac aaaatagacc cgcaagtaca
     5041 gtaccgcttc ctctaaaata tcggcaggag tctgtaaccc gctcatcatc gcatgggaaa
     5101 agcaaaagcc aaagacacca aggaggatgc tcagaagcaa aaagctgatc agtgacgtag
     5161 acacaatggt tttcattttg ccatactctt ttgcgccgaa acagcgactc acaagcacac
     5221 cagcaccgac accggcaccc agtgccacac aaatgaaaac attcgtcagg gctgcacagg
     5281 caccaaccgc tgccagtgca gaggaaccca caaactgacc gacgataatg gagtcagcca
     5341 tattgtatac ctgctgaaaa aagctgccga gaatcatcgg cattgcaaaa accgtcagcg
     5401 ctttcagcgg tgcatcggta atcaaatact catcttttga catgatccta ttcctccaca
     5461 gttccttcag cattcttcca tggcttcctc aactcgttca tctgcatctt ctggaagttg
     5521 gatggcatcc agctttcgcg tgaaaagttc aatcatcaca gaatactgat ttgtaattgg
     5581 gtgactaaac cgataaaact gcggcacatc ttctttcttt atatgcagca atcccatttc
     5641 ttcaagctgt ttcgatgccc ttgatataga agtaggtgtc agttccaagt tcttcgcggc
     5701 ttgacttgta gatagttcct gttcgcctcc aatcttaact ctttttgagt taagatacaa
     5761 tatttgagtt aagaacagtt gaaaatcaac attatcaaat attattttta cgcaaaaaaa
     5821 ccgatgcaac atgagcatta cacccacact gcaccggaac ttgttttttg catccgtttc
     5881 cagaatcacc ggcacgccca gccggtcgcc ctcggcaaag gcaatatcca gcacctttcg
     5941 catatagccc tgccgctgat atttctcccg cacacagaca agcccaacga aaatgtaggg
     6001 cttcctttcc ttgtccatgc ggtcttgcag ggaagcccct ccccgcttga tagcgatacc
     6061 aaactgcaga aggcgcttca tgtccgatac tgcttttgcg gcataccaat atgccgccca
     6121 ctattatctg acctccatgc agaaatcgca gcgatcattt ccgcgcccca gcgtcatgct
     6181 tctctcccag atcagcttcg gatgcagccc ggtatagc
//
LOCUS       DAWDHD010000149         6209 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_731_length_6209_cov_14.412252, whole genome shotgun sequence.
ACCESSION   DAWDHD010000149 DAWDHD010000000
VERSION     DAWDHD010000149.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 6209)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6209)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6209
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_731_length_6209_cov_14.412252"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1638 a         1413 c         1551 g         1607 t
ORIGIN      
        1 gcggtcgtat tgtgaccgct tatgtaccgc tacgctctgc gacagagcgc gagcgtgccg
       61 cgtttcttat accttgccaa gggctacggg gatttgatag catttcttaa ctaactttct
      121 atttacataa aaacccccgg ccaaataacc agccggggga ttgcactatt cttataattt
      181 tatgccagag ggaacttgaa atatctctta gcgttgttgt aggagatatc ggttacgatc
      241 tcggacagag tatcgtaatc tgcagggaat tcaccactct ctacccagtt accgatcagg
      301 ttgcacagga tacgacggaa gtactcgtgt ctggtgtagg acaggaagct tctggaatcg
      361 gtcagcattc caacgaatcc ggacaggttg cccaggttag ccagggagat catctgatcc
      421 tgcataccgg tcttgtgatc gttgaaccac caagcggaac cctgctggat cttggcaaca
      481 gcggtggaat cctgaaagca tccgaggatg gtaccgatgg cctggttgtc gttggggttc
      541 aggctgtaga tgacagttct gggcagctcg tcggttacga tcagagcgtt caggaagtca
      601 gccatctgag cggaaggagc gtagttgttg atgcagtcgt aaccggtatc gggacccagc
      661 ttctcgaaca tcagagtatt gttgtcgcgc ttacaaccgt aatgcagctg catagcccag
      721 tcaagcttgt gatattcctt gcctacgaac agcatgaatg cggtcttgaa cttcagttcc
      781 tcttccttgg tcagaaccat cttgttcagt ctctttaaga agatctcttc cagctcatcg
      841 tcggaagcgg gatagtacat tacatactcc agagcatggt cagatacgtt acaacccatg
      901 gatgcgaaga atgccatacg gttcttcaga gcttccttta agcttgcgaa ggagttgatc
      961 tcggtcatgc ctgcagcagc agccagcttc tccagataat ccagataatc gggcttctcg
     1021 atgttcatag ctttgtcagg tctccatgcg ggcagaacct ttacatcgaa agtatcatct
     1081 gcagccagct tcttgtgcca ctccagagaa tcaataggat catcggtggt gcagatcagg
     1141 gtaacattgc tctgcttgat cagattacga acgctcatgg aaggctgctg cagtttttcg
     1201 ttgcacaggt tccatacttc gtcagcggtg tttttgttca gaacgccatt gtaaccgaag
     1261 tatctctgta gttccagatg gctccagtgg aacagggggt tgccgatagc cttgcccagg
     1321 cactcagccc atttgcagaa cttctcgtaa tcggaagcgt caccggtgat gtacttttcg
     1381 tccacaccgc aagaacgcat gaaacgccac ttgtagtgat cgccgcccag ccatacctga
     1441 gtgatgttgt cgaactgacg atcctcagca atctccttgg gattgatatg gcaatggtag
     1501 tccagaatcg gtgttttttc tgcgtagtcg tggaacagct ttttggcggt ctcggtctct
     1561 aacagaaagt ccttgtccat aaatgctttc attttaaaaa tcctccatgt tcatatgagt
     1621 cagatccggt agggatccgg cgactatagt gataaatata gaaaaggggt gatttgcagc
     1681 tcccccgtgc tgtcataaac aaatgcttat atgtgcccac aagctacttt gtggtaccgc
     1741 gttttaccag ttcaccggag aatacagtct tcttcggcat ctcgcttccg cccagaacgc
     1801 ccatcagcgt tgtgatcaga tgctggcaga gtgtttccaa cttattgtcg atactggtca
     1861 gctccggatc gcagcagttc accagcatgg aattattata accgatgata gccagatcct
     1921 ccggaatccg cagcttcatt tcacgggcgt atttcagagc acccagtgcc agagaatcgt
     1981 cagcagcaat gacaccgtgg aaggtgattc cctttgcatg cagcttcagc agctggtctg
     2041 ccatagcggg aatatcttca tgagatccct gatagagctg caggaagctt ccgttggtca
     2101 gaagtccttt ttcctccatg gcggcacggt aaccggccag ctttttctta ccactgtaag
     2161 aagtggagtt gtagaagtac aggatgtctt ccactccgga acggatcatt tccatggtgg
     2221 catcatacat ggaagtaaaa tcatccgata cgatgctgta aacattggga gcgtccatgg
     2281 ctgcattcaa cagcatcaca ggcacctgtg cggcagcgcc gaggatgtat ttgttgtcat
     2341 cttccttctc gtagataaaa ttggaaccta ctaatataat gccgtccact tttttcgtga
     2401 tcagaaggtt catggaactt gctttggtat ccagatcata accggtacag cacaggatgc
     2461 tgtcgtaacc gttggcccga agcttctgct caatatagta gatcgctttg gcaaggtacg
     2521 gatccgaact gtcggcacac atgataccga tggtcttcat ggtgttgaga cctaatcccc
     2581 tggcgaatgc gttgggagtg tactcgtact ggttgatcac atccagcact tttttcttgg
     2641 ttttctcgct tacattgttg cttccgttga gtacgcggga aacagtagca atagagacac
     2701 cggccttttc agaaatatcg taaattgtca tctgtggctt cctctctgct tctatgtaac
     2761 agtgcgtaaa aagatatgta agaaaaatgt aagtagttac ataaaaaagc tatatctgaa
     2821 ttatagcgaa agacttctct tttttcaaga tactttttca acttctcata tgccaaaaat
     2881 ttatcaaagt gaaatattgc tgataaaagc ggtagtttca cgaaaataaa actttctttt
     2941 tttggtgatt gacgaaacca tttttttgaa gtaaaatgaa tcatgaaagt acttacataa
     3001 actttctaca attcagacca aagacaatgc cccgggcggt gtaacagatt cgttcgtaag
     3061 gcgtgccggg aaaagtaagt gcaggtaata gagtgccgta agacggtgca gataggatac
     3121 gctaagcgta gaatggagga caaaatggat atcttaaaca aggcaaagac cggtaaaaag
     3181 gaaagaccta tcaaggtggt acagttcggc gagggtaatt tcttacgtgg attcgtagat
     3241 tacatgatcg acatcgctaa cgagcagggc aagttcgatg gagacatcgt tctgatcaaa
     3301 cccatcgagt tcggtaacct ggatatgttc cacaagcagg actgtcagta tactgtatcc
     3361 cttcgcggta atgtgaacgg tgaagccaag attattaata gaatcgtaac cagtgttgca
     3421 gatgctgtag atacttataa tgaatatgac aagtacatgg gacttgccga gatcgatacc
     3481 ttacgtttcg tagtatccaa caccaccgag gcaggtatcg tatatgatga taccgataag
     3541 tttgaattag agcctcccaa gactttcccc ggcaagctga ccaagttctt ataccacaga
     3601 ttcaagacct tcaagggtga tatgagcaag ggtctggtaa tgcttcccgt agagctgatc
     3661 gatgacaacg gtattatgct gaagaagtgc gtaatgcagc tgatcgagct gtggggactg
     3721 ggcgatgagt tcaagaagtg ggtagatgag gcatgtgtat tcacctctac tctggtagac
     3781 cgtatcatta ccggttatcc ccgtgctacc gagaaggaag aatgggagaa gctgggttac
     3841 gaagaccaca tcatggtaac cggtgagccg tttgctctgt gggtcatcga atccgctaag
     3901 gacatcagca aggagttccc tcttcccgat gcaggacttc ctgttatctt cactgacaat
     3961 cagaagcctt ataagcagag aaaggttcgt atcctgaacg gtgcgcatac ctctttcgta
     4021 ctggcttctt acctgtgcgg caacgacatc gtaagacagt ccatgcatga tgacgatatc
     4081 cgtaacttca tgctgaagac catttatgac gaagtgatcc ctaccctgtc tctgccggag
     4141 gaggaactga agcagtttgc aggtgaggtt gtaaaccgtt tcgacaatcc ttatgtagat
     4201 catgcactgc ttgctatctc cctgaactcc gtatccaagt ggagagcaag atgtatgccc
     4261 agtctgttag gctatgttga gaagaccggt aagcttcccg ctcatctgac cttctccatt
     4321 gcagcactga tggcattcta ccatggcacc gagatccgcg ataaggctct gatcggtcat
     4381 agagacggtc aggaatacaa catcatggat gacaaagcag ttctggagtt cttcgcagca
     4441 aattgtgaga aggacaccaa gactttcgta acttccttcc ttggtaatga agacttcttc
     4501 ggccaggatc tgaacaaggt tcccggactg accgatgcag ttgtagcata cctggatgat
     4561 attaaggcaa atggcatgag ggcagcatta tgcaagattt cttaaaaatt aatgacaatg
     4621 ataatgtagt cgtagcatta aatacgattc ctgccggtga gaagatcaca gtctctgtag
     4681 gtgacggtca gaagaccgtt accgccagag aggagatccc agcaggacat aagatggcta
     4741 tttttgatat tcccgaaggc ggcgaggtca tcaaatacgg ttaccgtatc ggtaatgcca
     4801 aggagaacat cacagagggc agctggatcc atactcataa tgtaaagacc gctctgggcg
     4861 atctgctgga gtacacctac aaccccactc cggtagagga gaagaagacg gaagatgtga
     4921 cttttatggg cttcaaccgt ccggacggca aagtaggtgt tcgtaacgag atctgggtca
     4981 tccccaccgt tggctgtgtc aacaacgtgg caaccgccat tgcaaagcag gccaatgcat
     5041 tcgttaaggg cagtgtggaa gaggttatcg cattccctca tccctatggt tgttcccaga
     5101 tgggtgatga ccaggagcat acccgtaaga ttctggcaga cctgatcaat catcccaatg
     5161 caggtggtgt tctggtactg ggactgggct gtgagaacag caacattgat gtattaaagc
     5221 cttacattgg cgattatgat gagaaccgtg tgaaattcct ggtatgccag gagcacgagg
     5281 atgaaatcgc agattccgta gaggtgatca agggcctgat tgattatgct tccaagttcg
     5341 agagagagcc catcagcgta agcaagctgg tcatcggtat gaagtgcggc ggcagtgacg
     5401 gactgtccgg tattaccgcc aaccctctgg taggacgttt ctccgatctg ctgatctcca
     5461 agggtggcac caccatcctg accgaggttc ccgagatgtt cggtgcagag accattctga
     5521 tgaaccgttg tgccaacgag gaactgttcc accagaccgt agatctgatc aatgacttca
     5581 agaattattt caagagtcac aatcagacta tttacgagaa tccttctccg ggcaacaaaa
     5641 agggaggtat ctccactctg gaggacaagt ctctgggatg tacccagaag tccggaagtg
     5701 ctctggtcaa gggtgtattg cagtatggtg atacggtgaa gactccggga ctgaaccttt
     5761 tgtccgcacc gggcaacgat ctggtagcag ctaccgcact ggcagccgca ggagcacata
     5821 tcgtactgtt taccacagga cgcggaactc ctttcgcatc tccggttcct accatgaaga
     5881 tcgctaccaa ctcccgtctg gcaggcaaaa agtccaattg gatcgatttc aatgccggcg
     5941 tactggtaga ggataagacc atggaagagg agaccaaggc tttgtttgat ctggtagtgg
     6001 agactgcttc cggcaaacag gtatgcagtg aagcagcagg cttccatgat atggctattt
     6061 tcaaacaggg tgtaactctg taaaagatca tacacaaaac ggcgttcttt tgttacaata
     6121 aacgggcagg agaacgcctt tgttatatag gaggaagagt gaaaaataaa gtagattttt
     6181 gggcgcactt atataagggt tcataaaac
//
LOCUS       DAWDHD010000150         6198 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_733_length_6198_cov_9.706170, whole genome shotgun sequence.
ACCESSION   DAWDHD010000150 DAWDHD010000000
VERSION     DAWDHD010000150.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 6198)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6198)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6198
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_733_length_6198_cov_9.706170"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1384 a         1699 c         1292 g         1823 t
ORIGIN      
        1 tttaacatag agttttcagt atttcaagac ttttgaacga cctgtccatg aaacgcttcc
       61 gatattccga cgcgatctgt gaaagttctg caagaacctt gtccgatacg gtaaaggtat
      121 ataacttttc cacagaactc tccgctatgt aattcagtgc ataccgtgtg gattcttcca
      181 gtttgcgctc ctccggtact cccggatatt ctccgttgac aacgatggcc ttcagttcaa
      241 aaatactgcg caccagttca ttgggaagtg ccggtgcaca caaagcccgc agcgactggt
      301 agaccagttt catcatttct ttctcatcgt tgttctcccg gcagtaaaaa tccgccactt
      361 ccgcgaaata cataccgtag catgccccca cgtagtccgt catcagttcc ggaaaatagt
      421 tccggatctc tgcttccgat atcgtataag agttccgtcc ttcatacaga cgaaaagagc
      481 caaaagcaaa agggttggtc gctgcagcca gccggttacc gggctttctg gcccctctgg
      541 caaaagcagt gatcttccct ttttctctgg taagcaggca tacacgcctg tcatattcac
      601 cgaaaggtgt ctgttttaat acaattcccg ttacatacag ggattcctgc atgatccatc
      661 ctcccctgcg gacaggtctg tacgcccctc cgctctgcaa tccaccaggg ggcttttaca
      721 ttccgcacca gccaaaggtt tctccttttc cgcgctctgc aggtatttca ggtaatcctc
      781 cactctgccg gtatgttcaa attgctgcca gtaattcgtg aattgcatct tacgcatccc
      841 cctcttctat cagtgtatta tcctctgtaa aaatagggtc tgcaaatttc gtttttctat
      901 tcgcccatct caatgtcttt cggattatat ccgaagtttt tcaataagat gtcgctgtct
      961 ctccagtcct tcttcacctt cacccaaagc tgcagattga ccttcatttc cagcatatcc
     1021 tcgatctccg ggcgggcttg ggagccgatc tttttcagca tttgtccgcc tttgccgata
     1081 atgataccct tgtgggaatc tctctcacag atgatcgtgg cctcgatgtc acagatccgg
     1141 cctctctcct tcatgctttc caccgtcacg gcaataccat ggggaatttc ttcggacaaa
     1201 agtctcagag ccttttcacg gatcagctcc gccacgatct gacgcatggg ctggtcggtg
     1261 atggtatctt catcataaaa tgccgggccg taaggcagat attttaaaat acactggatc
     1321 agcacatccg tgttgtctcc cttcaatgca gacaccggaa cgatctcctg gaaatccagt
     1381 tctttgcgat aggtatcgat gaacgccagg atctcttcct ttttcacggt atccgtcttg
     1441 ttgatcacca gaatcacggg agtcttggtc ttcttcagct gctcaataat atgacgttct
     1501 ccggcaccga taaagttcgt gggctccacc agccacagga tcacatccac gtcgttcaat
     1561 gtatgctccg caacattgac catgtaatcg cccagcttgt ttttcgcctt atggataccg
     1621 ggagtgtcca gaaatacgat ctgtccctct tccagtgtca gcaccgtctg gatacggtta
     1681 cgggtggtct ggggtttgtt ggatgtaatg gcaatcttct gtccgatcag tcggttcatc
     1741 agtgtggact ttcctacgtt gggacgtccg atcagtgtgg caaagccgga tttgtatgcc
     1801 ggattttcgt tcattattat ttctcctgtt cttgtctttt tccctgacac ttttttgtca
     1861 gccgccggtc cgtccggctt atcttataga ttctcactca ttgctgtttt gtgtagctac
     1921 cgtattctct gctgtattct ctgccgtgtt ctgcaaagtc ttctgcattc ttgcttcctc
     1981 ttcggcgaat tttttctgtt cctccgcccg gatccgcttc gtgcgcttct tgatcttttt
     2041 caggatcagt acaatgatca cgatcacgat ggcccagatc accagatagg ggatatggat
     2101 cacgaaccag atggctgctt ccttaatacc ctcgccgata ctctttaagc tgtccataaa
     2161 gcctgcagaa atacgttccc atgtggtctc ttcctctacc ggagtataga cctctacttc
     2221 atcaatatag aggtatacgg tgctgtagtc aacctgatta tcataacttc tcaactggga
     2281 ttccatactt tccagctggt atctcacatc ggacaggcgc tgttcgatgg taatgatgtc
     2341 ttcgatgctc tccgcctgtt ccagcagctg caacagtctc tcctgttccg tctgcagggc
     2401 atccctgtga ctttgcagat ccacataggt cagtgtcaca tcctgtacat tctccgagcg
     2461 actggtcaca ttgcccagct ctgagaccgt attttggaac tcctccagtc tgtccttcgg
     2521 gatgcggatg gtcagattgg cattccttgt agaacgataa ctataatatg tactgccgtt
     2581 ataggcatcc agactttcga tatagccgcc gagctctgtt actttattct ctaccgcgct
     2641 taacagctta tcatactccc tggtctcgac atccatattc acggtcttga tcagtttccg
     2701 gtctgctgtg gctgcagtct cctcaggcac tgtagtactt gtgtcctgag catttcctgc
     2761 ggaatcgtac ccttctgtca tgccaaatcc atcttcataa gcggacgcct ccggtgcagc
     2821 tgcctccttg gaagcaacag cataatccgc atccatgaga tcactgattc cgtaactgcc
     2881 gttcgatgag ctgccgcagg cggtcagcga taatatcatg ccggcagtga gcaaccctgt
     2941 aatgatcttt gtttcaaaat ttcgtttttt cataaccctc tccgttctgc ggacggcatc
     3001 ctgacagcct gtcggatccg cctgctgttg actttttatt gttcacctct ataaacgaaa
     3061 taatatcaca aaagttcttt atttatttga tctttttacg attcagaacc acttttgcaa
     3121 aatcgcagct ttccttttgc cgttttaaaa aagattttcc accttcagga atctctcctg
     3181 ttcctccttt atcttagaag ccgtgcctac gacacagagc agatcctcct gcataaatgc
     3241 acggatatgt gcggacaatg cacggatatc ctcttcggta gccgccagta gttcgtcgcg
     3301 ttctctctgt aaagtggcat tatccagtcc tgtcatatag gcactcaggg aatacagtcc
     3361 ctttgccgcc ggattcatgg gtacgtccag atcactgacc gcaccgatga tatactgggt
     3421 catggtacgt tcatctgctg tgaactctgc aatggcatct gcagctttct cgtaagtttc
     3481 aatggtcttg cccagattcg gatctctgta ggagacaaag tagctgtcac cggatctgcc
     3541 gaagctgcac atgcagccgt aggcaccacc cttgacacgg acattaaccc acagatattc
     3601 ataccccatc atgactttga gtactcttaa tgctcccgta tattgcagtc cactcttgcg
     3661 gaaattaccg gcacggcata cgtaattgac ctgaccggag gttaagaaac cttcgttttt
     3721 ccgggaggtt tccggaatgt aatgttcttt ttccacgttt tccatataaa gtgctgcttt
     3781 gaaagcctcc accggagcat ctaaaagagc cacggctttt tcctcaccta cgaaatccac
     3841 catgaggttt tccggacgga agatcatttt cactaaggtc tgtaagattt ccaccaattc
     3901 ttcttttttc tcgtcaaaat gagcttccag atccgtgatc agacgataga acgggatacc
     3961 ggaaatctct tcgctcacag ctccgggaat actgccgtag gataaggctc ttccggccgc
     4021 cacactgtgt cctccggagg tcatctgtgc ctgcatccgg gacttgccct ccgccagaat
     4081 ctcatacagt ctcttggcat ccgtaaaatc actggtcatt acgatctcac gcatcagatc
     4141 gattgcttcc gcaaggcggt cataaaatac cttcgtcttc agttccagag tcagtataaa
     4201 ctgatccgta tcctgcagct tgccatagac attatttacc gcagccatgc cgccggtgac
     4261 cagattcatc tcattaaaca gatctccata agcataatgc tccgtattta acagtcccag
     4321 gcagcccttc aaaatgccga tatacgggaa atatttaccg gggatctgat ccaatctgaa
     4381 gatcagacgt aaatagccga tgccgtttgt aaacagatca tgatacaaaa gtgtggtatc
     4441 tccaatgctc cgcacttcgt tcaccggcag atttgcctct ttcttcatat cttcccgttt
     4501 taacagaggg attttctcca gatcttcctt tgcgtcttcg ctctcctgga attcggtcag
     4561 cttctggaag gtctccttca cgttctcgat ctcttccgga ctcatggcag ctttcacctg
     4621 tgccatcttt tcccgctgtt tctgctcctg ctcttccaga agtcccagct tcggctgcaa
     4681 gatcaccacg ctgcgatggg tattttccag cagatatttc tgtaccagac cctcaaaata
     4741 tccggtcttc actttccctc gaagttcagc gaaagtctcg ttggcctcga tgtgaatgaa
     4801 cggcagtctg tcatcgtaca gccagctgtc cagcacctgt aaaccgtaca taagtccctt
     4861 ggggtaggaa ccgaaatccg cctctctgta tttgaattca taataattga tgccggcaag
     4921 caatgccttt tcgtcgaagc cgtcctttac cagctttttc agcacttctt ccgttacctg
     4981 taaaaattcc tgttctttct ctacggaagt gtccttcgcc accacactga aatagggctg
     5041 gcggatacca ttctcataga tactgtagac atccttaccc acaccacagt ccaccagggc
     5101 ctgttttaag ggtgctcctg gagcggaaca cagggcatag tccaggatct ggaacgcaat
     5161 gtaaagcttc gggtccagac tattgcccac gcagaagttc tgggacaggt aggtattctc
     5221 ctcctcgtcc tctccctcgc cgatgggaca ttccctgaca atacgattcg gcgtggtaaa
     5281 tgccgcctct tccgtcactt cggaatccac ctcaagggca tcgtaggcag acagataatg
     5341 ctcatcgata aaggtcagtt tctccgccat atccatattg ccatagagat agatatagct
     5401 gttggaggga tgatagaatt tccggtggaa attcaggaat tcttcgtagg tcagctcggg
     5461 gattgcttcc ggatcacctc cggattcgca gccgtaggtg gtgtgaggat acagggaatt
     5521 catgatctcc cgctccagca catcatccgg agaggaaaaa gcacccttca tctcgttata
     5581 gaccacaccg ttgactttca gctcctcgtt gtcaccttcc agctcataat gccagccttc
     5641 ctgtcggaaa atagattcgt ttttgtaaat attcgggtaa aaaaccgcat ccagatagac
     5701 atgcatcagg ttctggaaat ccttgtcatt acagcttgcc acagggtata cggtcttatc
     5761 cggataggtc atggcattta aaaaagtgtt caaagaacct tttaccagtt ccacaaaagg
     5821 atccttcacc gggaattcct tcgagccgca taatacggag tgctccagga tatgtgccac
     5881 gccggtggag tccttcgggg tggtacggaa accgatgtaa aatactttgt tctcatcgtc
     5941 attggatacc agtgccactc ttgctccggt cttcttatga cggcacagat aagtcacact
     6001 gttgatgtcg gggatctccc gtttctctat gatctcgtat gtggataaat cttctactct
     6061 catttattta cgtctcctac tttttagctt aatcgatttc tccgctcttc gagctcccga
     6121 aatcgtaacg cccattcgca attcggacta tcgtccttct tgctcatgtt cgtttgctcc
     6181 cctcatagaa agccttcg
//
LOCUS       DAWDHD010000151         6158 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_738_length_6158_cov_16.746846, whole genome shotgun sequence.
ACCESSION   DAWDHD010000151 DAWDHD010000000
VERSION     DAWDHD010000151.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 6158)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6158)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6158
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_738_length_6158_cov_16.746846"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1693 a         1129 c         1689 g         1647 t
ORIGIN      
        1 tattttgtct tctagctgtc taaactggag attaccttga aaaaaggaag tgccttttac
       61 atgatgagca ttctgctgtt gggggtatcc accttctgca ccgacggatg ggcgatcata
      121 ggtctgaaca agctggcggt gtttctatta gtgatgtgtc tgctgctgaa tcaatatttt
      181 gatacgaaaa agtggaatct tgggaaatat gtgggaagca tctgtcaatt ggtggttatg
      241 agcttcgggg aactggagaa gcctttttcc gatggcaaag cctattttcg tgaaaaggga
      301 aaagtaaata agaaggtatg gtacgggctg ttgggagtag tgatcgcatt gccgattgtg
      361 ctgatcgctg ccggactgtt aagctctgcc gatgcggtat tccgtaagat gacaacggat
      421 ttcatgaact ggatccgccc gggcaatata ttcaacgtag tcatccgggt aacatttctg
      481 ttctttactt cctatgcact gacttcttat ctgtgtaaga gaagcatccc ggaagaagtg
      541 aaggatcgca gaaagggtga gccggtactg gcaattacga ttatgtcatt gctgtcactt
      601 ttgtatctgt tgttttccgg tattcagatc ttcggattgt tcttggggaa aatgcagctg
      661 ccggagggct atacctatgc acagtacgca agagagggct tcttccagct gttggctgtc
      721 agcattctga acctgatcct ggtattggtg tgcctaagct tttttaggga gagtagggta
      781 ctgaaagtaa ttatgacgat tatgtcatta tgtactttta tcatgatcgc atccagcgta
      841 atgcgtatga tcatttacat ccggtattat tatctgactt tcctgcggat cttcgtattg
      901 tggatgctgg cggtattatt cgtcatgttt atcggagtac tgatcaacat ttatagggaa
      961 agcttccctc tgttccgcta tggtgtagtg atggtgaccg tgctgtatct ggcattgtcc
     1021 ttcagccatc ctgactatat tatagccaga gtaaatattg ccaatgcacc cggcgcggac
     1081 ggtacgggct gggaagaatc tgtggcacag gattacggag acgatttttt cgccggaccg
     1141 tttttccggg gagcggaata taatgactac tcttatctga gtgatctcag tgcggatgcg
     1201 gcaccggcca ttattcctta tatggagagc ctggggtatg attttggcgc ctatgaactg
     1261 gagaacccta cggatattat gagcgaagat acgcgggctc agtggtgggg gagttatcag
     1321 ccgaaaggat tcggctatta ttatctgaac cataataaga agcgcctgga gcagacatct
     1381 ctgcggacat ttaatatttc cagatatatg gcgcggaaac agatagaggc gagaatcgat
     1441 ggaaaataaa gaaatgaata cggaaaaaat aaagttttat aaaaagcatc cggtgttgct
     1501 tgcatggctg atttctatat ttatcggact gggctatgcg ctttttacga tcatagcctc
     1561 tgttattcat tatgagcaaa gagattatgt atgggagatc attaaggctt ttacagaaat
     1621 gtttacctgg gcaattttaa tgggagcggt actggtattt cctgtggtgc tgacgatcag
     1681 tgaagtaatc tgcctgatct gtgaggaacg agggcacccg gtgaagggag actggctgtt
     1741 tgaccagcac gtattttggc tgggtggatt ttatgaaatt tgttatttag ggttgattat
     1801 ggatgtgaca tctgctgact ggcagacaca gttaagcaat tccaataaac atacaccgat
     1861 ttacagtggt tccatggtta cttttatagt actcctgctg ctggcattta tagggtatga
     1921 gattctgcag agcattccgc ttcggaagct gccgcccctt gtgacagtcc tgtcgatttc
     1981 cgcaatgtac ctgggactgc tggagctgat cctgttcaca gtacagatat tcaagccgac
     2041 gattttgctg gatggatatt tgctattatt tccactgtgc tgtgtgctgc tagtggtgcg
     2101 actgcttctg aagaaaatac gggagtggaa tgcactgatg cagaatgcgg aggcggaaca
     2161 ctttgggacg gggaagattt atcagaatcc catgctgcgt tggtgtgaca gcatattgcg
     2221 gaaggctgca tggtggccgg tgctgggact ggtattgatg ttcccactgt tggggatcct
     2281 gatcgcaatc ctcatgctgt ttgggcaggc accggattct gtgattaaag cattcacgga
     2341 gaccagcgac tggaatctgt ctctcaggca ggcaccgcag aatgtcatgt atgatgaaca
     2401 ttatctctgc acggtagctg ccggcggtca tgaaaaagtc gtaaagccta tccgtctggg
     2461 gaggcggcat gggcatgaag tcattgtgaa ccgtcagttg tgcattgcga acgcttttga
     2521 acaggtgttg gaagaacgga ctcccggctt ccaccgggca ctgcgtcatt tttatgatac
     2581 ttacgggttc cctgtggcga gactgatcca cagtaaatac acggcggatc tggtgtattt
     2641 tatcatgaaa cctttagaat ggatcttcct gtgtgtgctg tacctgacag atgcacaccc
     2701 cgagaaccgt attgccgtgc agtatatcgg gaaaacggca gcgcaggtgg aagagttgta
     2761 tttgtaaaaa tagtggcgta cgatgcaact atgatgcaat ggtatgttaa attctttact
     2821 gtaatggacg ggggaagcgt tcggttacaa taaatacata aggagagata caaaatgagg
     2881 ggcaaattaa tagttatcac aggagttatg gcactggggt tgtctgcctg cggagcacag
     2941 gggagtgatc cggtgggagg gagtagttca gaggttatcg gatccgcaga ggctgcggta
     3001 acggaagaga gcacttcaga gactggtgaa tccatggaag cgacagtatc cacggagact
     3061 gtttccgaca ctacggcatc cacgacaggt ccttcttacg aagatgagga cgcatggctt
     3121 ccctgggaag actacggaga atgttccaac actgtgaaag acttctacaa tgacgatgag
     3181 aaaaagacct attactacga aatggatgag ttcttttttt ccgatgagaa gtatgccaaa
     3241 gtgaatgatt atctgcagca gatgtatgag aattacagaa cccagtatga ggaggaaggt
     3301 gagaatcaca ccggagcgta tgagttagtg gatgaaactc tttccgaggg acagcgatac
     3361 gatgataatt atctggtatt caatggcatt actttagcgg atgacgagta tgtcagcctg
     3421 cattttaatg acacggttta ttatgcagga gcagcgcatc cattatccta ttacataccc
     3481 gtgacgatct ccgttgccac cggcgaagaa gtcaccccgg aagaagtttt ggggaagacc
     3541 tgggacgaga tccgggcagc aggctctatt gaagaagaaa acgaagaaaa ttttaatgcc
     3601 aattacggct tttacatcac agagaaggaa ctgtgctatc tctatcgcac taatttcttt
     3661 gtagaagaga tccggattcc ccggtaaaag aaccggattg aactggcagg gcagaagagg
     3721 tatacttatt ttcggaaacg aacggaggta ccgggatgga aaagtttaac ccttcagagc
     3781 tttgtgcaga cattaagatc tatgattata agaaaaaagt aaaatatgat gagaaatccc
     3841 tggtgatttt tgagaaaaca gggaaaatga taacagccgg aaaagagtgc gaaggcatgc
     3901 tttacgctct tccggctaat tctattggtt tttctccgat cgtattggga agagtcagcg
     3961 actatacctg tgcggaaaag atgctaaagc agatgctgtg ccggtatctt ggcaaggcat
     4021 catttacggg atacggagaa gggcttattt ttattcacga gaagctgaac gaagtggaaa
     4081 tgaaagtcta tttcgatctt ctgtatcagg cgggagccaa aaatgtggtc tatgcagatg
     4141 aaagcgtgaa aggaatcccg gagggaactc cctgggagga tgtgatctgg ggaatgaaga
     4201 acacttataa aaatcttcgt tttgccgtgg agatcacgaa ggaacagccc atggattatc
     4261 tcagatattc gctagcggag ttggcagaga actgtaagcg gtggggactg gaagaggaat
     4321 ataacaaaag agtaatggag atgaaataat ggtattagat gttttaggat tactgtcagc
     4381 ctgttcgtat gctttggact gtgtggaggc agaactggta catgtgtctg acaaacatgc
     4441 gaagcgtgtg gcatacatga gtgtctgcat ggcagagcag ctggggatca gcgatgagag
     4501 cctgcaggat ctggcagcat gtgccctgtt gcatgataat gcactgacac agtatattca
     4561 ggaagaatta cataaggatg ttgcaaatgt tccgcaagct tctcaggtag gaattcactg
     4621 taccctgggt gaaaaaaata tacagagact tcccttccat acggatgtga agaatgtaat
     4681 tctgtatcac catgaaaatg ccaatgggag tggaccgttt ggaaaaacat gggaagagat
     4741 tccgcttttt tcccgcatca tccatttaag cgatctgttg gaccgggcgt acggtgcaaa
     4801 gggcttcacg gaggatattt tcaataaagc ttgtggatat cttcacaaaa acgaaggaac
     4861 cgttgtggac aaggaatgtg tggatgcttt cctgcaggcg tttcccctgc cgcattttct
     4921 gacactggga gaggatagct ttgagaaaac tctgtgggag aaaatccccc gcatcaaaca
     4981 ggagctaagc ttcgcacaga tcaaggaact ggcccgtttt tttgcacaga tcgttgatta
     5041 caagtcaccc tttaccagca cccattccat tggtgtggcg gaggatgcgg agcgcctaag
     5101 ccgctatatg ggctttgatg aagagaccgt gcagaaaatg tacctggcgg gcgctttaca
     5161 tgatatcgga aaagtggcgg taggtaatga gatcctggag aagccgggaa ggcttacgga
     5221 ggacgaattt gctgtgatga aacatcatgc ggcatatacc tattatattc tgtcaggcgt
     5281 agatgatttt gacgagatcc gcgactgggc ggcattccac catgagcggc tggacggaac
     5341 cggatatcct ttcgggaaga ccgccgcaga actgaacacg caggagcgca tgatggcctg
     5401 tatagacatc tatcaggcac tgacggaaag tcgtccctat aaacagggca tgcctcacga
     5461 aaaagcttgt gagatcctgt gggatatggc gaataagggc tggctggatg aaacgattgt
     5521 gaaacaggtc gaggattgct ttcggggata aaagatgtga gggcgattat tgcaggatta
     5581 atatgacgaa gatataggga aggtataaag atatggacaa ctttatagaa gctttgtatg
     5641 gacaaccgga aggaactatt ccggaggagt atgattggtt tgctccgctg ttgggtgatt
     5701 gggagtttga ttataaagat aagtttatag atggtgatca taacaggcca cagcgtcaat
     5761 tagagggaga gtggatcttt aggcgggtat tgaacgggat aggcattgaa gatatgttca
     5821 tatgtccttc cagagcaacg agggagactt ctccacagcc ggatggtgag tatggtgttg
     5881 gactcagaat atataattca aagactcatt gttatgatat ggtatatgca tgcaagagta
     5941 aatttacaaa tttgtgtttt accatggaaa atggaatatt gacgggaact gttctggaaa
     6001 atcccaatcg aaaatggcga tttgtggaaa gaaatgaaaa tacatttcat tggcagaata
     6061 ttaccattca ggagaatggt gacattactg taaattgtga ggttttcggg aaaagaaaaa
     6121 gcgaggaagc gtaaaacatg aacgttatag acattatg
//
LOCUS       DAWDHD010000152         6117 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_746_length_6117_cov_10.349720, whole genome shotgun sequence.
ACCESSION   DAWDHD010000152 DAWDHD010000000
VERSION     DAWDHD010000152.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 6117)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6117)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6117
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_746_length_6117_cov_10.349720"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1349 a         1701 c         1243 g         1824 t
ORIGIN      
        1 cgttcagatc caaaataccg atcaccgcga tcaacactcc ggctcccacc gccaaataaa
       61 atcccgttgc ggaaaaagct ttcgctcgct cctttttatg agctcctaac aaccggctga
      121 tattgcttcc ggcgccatga ccgaacatga atccgaacgc ctgaataatt gccatcagcc
      181 caaacaccac gcctaccgct ccggtagcac tggtgcctaa ctggcttacg aaccaggtat
      241 ccaccatatt atacagattc gtgatcagca tactgatggt agtcggcagt cccagttcca
      301 ggaccaaccg tgctaccggt tcctcggtga gccgtttata ctgtttttcc tcttttgaca
      361 atgttgtatc actcatatca catagctcct atatcattac cacagtttcc attcggttct
      421 ctctatggtt ttacactatt ctatgccaca gtaatccgca ggctgcttct gcccggtttg
      481 aaatcataat ccgtacccat tcatcgtatc gccaaaatag cgtctgccct accggcacga
      541 cgctattata gatgattctc tttgaaaatg taagctcttt cagctgcttt tttactaaaa
      601 ttttatgttc agcctcacag cttctcactg aaatagctct tatagccctc cccgaagatc
      661 tccgtggcac tggtgacgat cataaatgcc aggggatcct cttcctttac gatctcctct
      721 gccttgggag aaatgttttt cttcatggcg cacatgatga cgctcttctc cttgccgctg
      781 taagcaccac tgccctgtac ataagtcaca ccaatgtcca gctcctccaa caatctccct
      841 gcgatttttt ccgcatggtc gctgataata tagatcagct tcgcctcatc acggccatac
      901 aataccacat ccagcaccac tgatgtgata atgaccacga taccggcata cagtgccttg
      961 tcaacattcc ggaatacaaa tgccgatgct atgacgataa ccacatctgt caggaaaaac
     1021 aatgctcccg tcttgaggta cgggaacttc aaacgcagta atttgataat aatatctgtt
     1081 cctcccgtag tggatccggc cttgaacacc cagcccatag atactgccat cagggcactt
     1141 ccagccaaag aggcaagtaa tggatctgtg gtcactgctc ctaccggtgt cagtaggttt
     1201 gtaaacagtg aggtcattgc cgtacaataa atcgtagaca aaataaaccg caatccaaat
     1261 ttccagatac cgaccagcag gatcggtatg ttgatgagta acatccaggt acctgtctca
     1321 attccgaata aacggtttaa aataatggca ataccggtga tacccccggg tgccagggag
     1381 ttcggatcca aaaacatgct gacggatacc gcatagacaa aggatgccac cgtgatcatc
     1441 acataatccc agagcatctg taagggtgtc tttttgtcca gtctctgtat ttccagttct
     1501 ttttctgtct tctttgccat aacttttttc tccattccat acaaaagccg catatctata
     1561 atatgcggct tttatcggtg atacattcac ttcttttcaa atcttaaaac ttagatattg
     1621 gctcttacca ctttgggctg atagccttct ttttccagag cggcaatgat ctcattctta
     1681 tgctcggtgc cgaatgcttc cagtgtgata cgcagctcca cagcagcact gcggttgatg
     1741 gatacaaact ggttgtgctc cagcttgatg acattaccgt tgactccggc aataataccg
     1801 gatactctgc acagctcacc gggcttgtcg ggaagcagta cggatacggt gaagatacgg
     1861 tctcttaaga tcagaccctg ctgtactacg gaagacatgg tgatgacatc catgttaccg
     1921 ccactcagga tagatacaat acgcttatcc ttcacattta actgctttaa ggctgctacc
     1981 gtcagcagac cggaattctc cacgatcatc ttgtgattct ctaccatatc cagaaaagct
     2041 actaccagat cctcatcctc tacggtgatc acatcgtcca gattcttctg taagtacggg
     2101 aagatcttgc agccaggagt ctttaccgcg gtaccgtctg cgatggtgtt gaccttgggc
     2161 aatgtggtaa ccttgccgtt ctttaaggat acctgcatgc agttagctcc tgcgggctct
     2221 acgccgatga ccttgatctt cggattcagc agttttgcca gtgtggaaac accggttgcc
     2281 agtccgccgc caccgatagg aaccagaata taatctacca gcggaagctc tttgaatatc
     2341 tccatggcaa tggtaccctg accggtagct acggtcagat catcaaaagg atggatgaaa
     2401 gtatatccgt gctcttctgc cagctcatat gcctttgcgc aggcctcatc atagacatcg
     2461 ccatgcagta ctacctctgc tccatagcct ctggtacggt ttaccttgat caaaggtgtc
     2521 gtggtgggca ttacgatggt tgccttgctt ccatagcatt ttgccgcata agccacgccc
     2581 tgggcatggt tgcctgcaga ggcggtgatc agtcccttgg cgcgctcttc atcggtcaga
     2641 gtgctcattt tgtaataagc gcctcttaac ttgtaagctc cggtaaactg taagttctcg
     2701 ggctttaagt atacgcggtt gccggtctgg gcactgaaat aatcactgta taccagtttg
     2761 gtctctaaag taactttttt aacgatctca cttgcttctt caaatttctc taaagtcaac
     2821 atttcttcct gcatttttct tcccatgcct ttctgtcaca ctttctttgg tctgcctgtt
     2881 tattattctt ccgcgtctgt ctctccggct tcttcctccg gcagatgcag ttctccgatg
     2941 tcatccatat tcacatcaaa caacgcattt acaaattcat cggaattgaa tttctggaga
     3001 tcctcgatgg attctcccac accgatatat tttaccggaa ccttcagttc ggactggatg
     3061 gccacagcga taccgccctt ggccgttccg tccatcttgg tcaggatgat acccgtaaga
     3121 tccgccacct caccgaattc tcttgcctgc accagagcat tctgtcccgt ggtgccgtcc
     3181 agcacgatca gattctctct gtgggcattg gggaattcct tgtcgatgat acggttcatc
     3241 ttacgaagtt cttccatcag gttcttttta ttgtgcagtc tgccggcggt atctacaaga
     3301 agcacatcca cgcctcgtgc cttcgccgca ttcacggcat caaacaccac gctggcggga
     3361 tccgcacctt cttttccgcc gatcatggga acatccgctc tggatgccca ttctgccagc
     3421 tgatctcccg ctgcggcacg aaaggtatcc gctgcggcga taaggacttt tctgccctca
     3481 tcctttaatt tacctgccag cttgcccacg gaagtggtct tacccacacc gttgacacca
     3541 atgaccatga tcacggactg ttccttctcg aaatcatagg cggtctcatc cacctgcatc
     3601 tgctccttaa tactttcgat cagaagctgt ttgcattcgg aaggctcctt gatatgcttt
     3661 tcttttacct gctgttttaa tctctccaca atggcagtgg tggcattgac accgatatct
     3721 cccataatga ggatttcttc cagttcttcg tagaaatcat catcaatggc ggaaaaaccg
     3781 ctgaagaccg aatcgattcc ccggacaatg ttatcacggg tcttggtcag tccttctttt
     3841 aaccgggcaa aaaagccttt cttttcctca ctcatccgat ttcctccaat cagcattcta
     3901 tccagaaaaa cgcttgcgtt tttcttgtat gaaacttaga acttcttcac gaaagagcct
     3961 ttgctctgag tgattagaag ttcttttcat actctaatca tccagatctt tatcaatgag
     4021 gttaacactg actaaagtag atacaccctt ctcctgcatg gtgataccat acagtctgtc
     4081 cacctgttcc atcgtaccgc gtctgtgggt aatgacaatg aactgggtgt tcttggtcag
     4141 cttatgtaaa tactttgcaa aacgtcctac attggagtca tccagtgcgg cctcgatctc
     4201 gtctaacagg cagaacggag acggcttcag gttctgaatt gcgaacagta gggaaatagc
     4261 ggataatgcc ttctctccac cggagagctg catcatgttc tgcagctttt ttccgggggg
     4321 ctgtgcgatg atgcggatgc ctgcctccag aatatcctca tcctccatca gttccagcgt
     4381 tcccttacca ccaccgaaga gttccttgaa taccttatcg aactctctgc tgatttctgc
     4441 gaatttctcc gtgaactgtt tacgcatggc ggtatccagt tcttcgatga tgccttccag
     4501 cgtcttctcc gcttccacca gatcatcatg ctgggtcttc atgaaggtat agcgttccat
     4561 caggttctta taatcctcaa tggcattgac attgacattg ccaagcttac ggatctcatc
     4621 cttcagaccg ctgatctctc gtttcatagc aggcagatca ttcatctctt cgttgcggag
     4681 tcctgccgca tcgcttaagg tgatctcata ctcatcccac atgtaattga tctgaccttc
     4741 gatgccgtcc tccagctttt tcttctgttc gcccagacga tagacttctt tgtccagagc
     4801 attcatgcgt tctgacattt cttctctgct gcggaagaaa tctttctgtt ttgcagacag
     4861 ttcttccttt ttcgcaaggt cttcgtccag cttcttcctg gaagcatcct gattctcatg
     4921 agaagcagcg atggtcttct caatctccag gatgttgttc ttcttacgtt ccacttcctg
     4981 cgcattttcc gtcagagctt ctaaaatctc attcagctct gcctgtgcac gttccagctc
     5041 gccgtctata cgatccacat ttgcctgctt gtaatccagt gcctgacgca tcttctcgac
     5101 ttttacttcc cattctcctg cgtgggcgga agcttcactc tcttccagtc tacggccttc
     5161 cagttccttc tggaagccca ggatctgttc ctgcgtcgtc ttctccagtt cttcgctggc
     5221 agccagttcc tgcgtaatgg attccttgcc ggaacgaatc tcgaagatcc tggtctcgat
     5281 ctcctgctcc tccagcttca gagactgtgc actctctgcc tcttcttcca tacgctctct
     5341 tgcctgggag atgttcaatc ttgcggtatt ctgctcaatg gacttgcgct gcatttcggt
     5401 cttcagtgct tccagatcca tacgaagttt atttctctta ctcttggtgt cttcgatctt
     5461 ctgattgtaa gtcgtgatat cctcagagag ctttttcacc tttttctcca gttcgtccat
     5521 ctcacgacgt cttcccagga gattgctgtt gtttttaaaa gcaccgccgc tgatggcacc
     5581 accgggaact aagagttcac cttccaaagt gaccatgcgg atgccgtaat cgaattttct
     5641 tgcgatcttc acggcattgt ccacattgtc taccacaacg atacgtccca gcatagcctt
     5701 cgccacattg cggtattctt cttctgtgcc taccagttcg tctgccatgc cgatgacacc
     5761 tttttcctta agtgcctccg gattcttgaa ttcctggggc ttggtgatac tggtcagagg
     5821 caggaatgtg gctcttccga gacggttctg ctttaagaag ccgatcatat gcttggcggt
     5881 ttcttcgtta tccgttacga tattctggat attgccgccc agcgcagtct cgatggcggt
     5941 ctcgtatttt gcctccacct gaatgatgtc cgctacgaca ccgatgatgc ccttttcttt
     6001 ttccttctgc tccatgacct ttttgacgct gccgccgtaa ccatcatagc gctcggtaat
     6061 attggacagg gcttctaact tggaatgttc gtgatgaaaa cggttctgtg tctcccg
//
LOCUS       DAWDHD010000153         6076 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_752_length_6076_cov_9.876765, whole genome shotgun sequence.
ACCESSION   DAWDHD010000153 DAWDHD010000000
VERSION     DAWDHD010000153.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 6076)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6076)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6076
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_752_length_6076_cov_9.876765"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1585 a         1698 c         1266 g         1527 t
ORIGIN      
        1 aacagcagac cggcacaggc tacaccgaag caggcataag gaagcaatgc ggaattgtcc
       61 gcaccgtctg ccagaggtgc gaatgcggca taaccgccta agaatgcaaa ggaagaaccc
      121 agaaatgcgg gtacctttct cttggtcagc agatggaaca gtaaagttcc cagacctgca
      181 aataacagtg ttgtggaaac atccagacct gtcagcatgg gtaccagaat agtggcacca
      241 aacattgcga acatgtgctg aagtcccagc agaagcatct tgggcacgcc caaggatctg
      301 gcatcgtaaa tgccgttttc gccgagtgta gttttctcgc tcattttttt cctcctgtgt
      361 catagacatg tgaacatgta actatttttc gtcgcataaa ttcgggcaga tagtcctcta
      421 tctgcccaca cctatgtgac tttattagtt acatgatagc ttttcatcat tccacagtca
      481 agaaaaaaat tattccaccg catgctcctt cagaaaatcc agccatatgg gaatatactg
      541 tgccttcaga tgcacaccgt cataagtata ggaagccacc attccaccgg tctcgtcaca
      601 gatcagcgga tttacatcca ggaaaaatat cttccggtca tccgccagct ttgcaatttc
      661 tgcatttctg gcttcgattc cttcgttggt aatataatcc ccctgttcag agcgttccgt
      721 tgttactttc ataatggcct gcagatagat caccgcatcc ggctgcaatt cctgcaaatg
      781 ctgtaccgct tccccgtaag ccttcataaa agattccact gtgccggtac ccatttcgtt
      841 gataccgatc atcagataga tcttggcaaa ctgcttctcc gacaaggctt cctccaccgt
      901 gatctttttc ttctgtccag gcaccgatac aatggcactg tcgaacatct tgtagactgt
      961 cagtccggtg gaagcataaa acgtggatgt ctcctccagt ccgccgtact catacatgcc
     1021 taccgtccgg gaatccccga taaataccgc atcatcaaaa taactgtcat ccaccgtgtg
     1081 atagaccact tcctgcggta tctcttccac aggagccgtc gcggcgggat cctgcgtctc
     1141 tgttccggtg gtctccggca ttgcggcctc ttctcccgta gcgggactct gcaccgtacc
     1201 catcgtctga tccgtctggt tcccgtcact cactgtatct ccggtctgta cgttttcccc
     1261 cggcacattg ccggatacgg atcccgttgc atcactctgt tccccggata ttcccggcca
     1321 ttttccttcc accagctgct ccgccagaag cactcctttc gtatagatct cctgcagcgg
     1381 ttccgtatat acctgccagt tatccgtcca ggtaaagaat acaattcccg ccagcaaaag
     1441 acatcccaaa aaggagtatt tatgcaattt ttccttcatt ttcgaccaaa tatttttatt
     1501 ttctgcatga ttcattgcat aatgaccttt tcctaaaagc taaaatacat aaaaggatta
     1561 tttccttcta ataaaagtct ccagatgcaa taccagaaga gccccgccag aagcaccttc
     1621 accggcagct tatcctgcca cttatggtac agcttctcaa tgaccggtgt gcaggccagt
     1681 ccagagacca gaagcagtcc tccataggta ctgagtgcct tccaggcatc ctcgtaaccg
     1741 ccgctgaagc ccggtaccag ccacagcatc ttatccagat agacctgtaa ctgtgacagg
     1801 tccgtgatag caaagcacat ccagctcatt gggatgacca gccacagata aatgtgcggc
     1861 agtaccttca ttttatgtac aaattttaat ctgcccagct gccgttccag tacgatgcag
     1921 atccagagca gtccgcccca gcagaagaag ttccagccgg caccatgcca gaaagcggtc
     1981 agcatccata ccaccaggag gttaaaaatc gtgcgaagct ctccctgtct gctgccgcct
     2041 aacggaatat acacatatcg gcagaaccac tgtcccaacg tcatatgcca tctgcggtaa
     2101 aaatcccgga cacttctggc catatagggt ctgcggaagt tggccggcag ttcgatcccc
     2161 atcatccggc ccagtcccat ggccatcagg gaatagcccc agaaatcaaa atagatctcc
     2221 atggaatagg cgatggctgc cagccatgct aacggtgtcg agatgctttc aaatccggtc
     2281 acctgcacct cgtgccacaa gattcccacc cggtctgcca gtagcacctt ggccgccagt
     2341 cccagaataa acagcttcat accgtcttcc agcccccgca ccgtgaaagt gcgctccgtc
     2401 aggctgtccg agatatcgcc atatcttgtg atcggtccgg agatcagttt cgggaacatg
     2461 atcacataag tagcaaactt caaaaaagaa tactctctgc tcacctctcc ccggtacaca
     2521 tcgatctgat aagacaatat ctggaaggtg taaaagctga tccccagcgg cagccccgca
     2581 gagcctcccc agtccttgaa ccagagcagt accgccagat tcgcgccaag tgtcaggatc
     2641 agcagaagct ttttccgcca caacgtctta ggactgcgtt ttgccagatg aagccccaga
     2701 aaataattgc acaatataga tatcattaaa aggggaagat acctgacgtc tcccctggca
     2761 taaaaaacaa tgcttcccac gagcagtgtc aggttcctgt atttgcgagg tgtgatcccg
     2821 tagacgatca gaaagatcgg caggaaccac agcagaaatt caatactgct gaatatcaaa
     2881 aaaatcacat ctttctattt ctgtttatct gtcttactat catagtagct tgaaaccgtt
     2941 tctattacaa caaaacaatt cttaatttta aatgacagaa ttttacaact gcttaatctt
     3001 tttgcaattt ttcaccgcat cgtttacaat agttttaaca ccacagggct gcgcccgaga
     3061 aaggatattt tccatgaaag aacagttata taccatccct ttaaacgatg ccgtaaatgc
     3121 acaggacgaa tgccctttct gctttatcca ccgcagtatt gaacaggatc ttctggattt
     3181 cgtgttaggc tccggttcct cttatatgga agcggacatc cgcgaacaaa ccgacaaagc
     3241 aggcttctgc cggtatcatt ttcagaaaat gttcgactac ggcaatactc tgggcaatgc
     3301 ctggatctta aagacccact accagcgcat gatccgggaa atgcagcagg aatttgcctc
     3361 cttccgcccc ggcaaaagct ctctcctggg taaattcaaa aaagtcgaag gcaatgaaaa
     3421 taccatcggc atgtgggtcc gtgcaaaaga ggattcctgc tatatctgtt cccagtataa
     3481 agatacctac gccagatatc tggacacctt tttctacctg tggaaaaatg atgaaaactt
     3541 ccgcaataaa gtcataaacg gcaaaggctt ctgcctgccc cacttcggcg atctctgcga
     3601 agccgctgac cgcaagcttt ccgataagga aaaacaggac ttttacgatc agttgctgcc
     3661 ggtcatggaa tccaacatgc agcgcatttc cgaagacatc tcctggctgg tggaaaaatt
     3721 cgactatcgc aacaaagacg ccgactggaa gagctccagg gatgccatcc agcgcggtat
     3781 gcagaaatta aaaggcggat atcccgcaga tcccgcctat aagatgtcta aataaccaca
     3841 aaattttatt caaaaatacg atgcaattat gatgctattt ttccgtaatt tcttcgtcag
     3901 agggctgtcc ggaccgttcc gccagtcttc tgcttaattg cagatactcc agaatttcct
     3961 catacagcat ctccggctga aaggcatctt ccaggattct ctcgttcatc aatcccttaa
     4021 tggtcagttc catcatctta cgaagtttca ccacgtcttc cgcagccgca tattttccca
     4081 gatccgctct ggcataaata ttctcataag cctcttccat ctgctgtttt cgctcttcta
     4141 ccgccaccag tgcctcattg acattctctg cctgtgcccg gttcaggaac ggctgcagat
     4201 aaggatagcc gcacatggca tgcattttcc cggtctccac ctgctgcatc agagtgaaca
     4261 gatccgtctc cttcgcatct accgctgtgg acaattccag caacagatac cgtacactgt
     4321 agtcatagac aaaagcatag actcccagct tgttctcaaa ataatgaaat aataatccct
     4381 tactgatggc tgcctcccgc acgatatcat ccgtactggc atgacggtaa ccgttcaggg
     4441 caaatacctt cagagctgca ttgatcatcc ggtcctgttt ttcttttttt aaatcaaaaa
     4501 atttctcatt catatcgcaa catatccctt ttcattttca tcaataaggt ttattatgta
     4561 actgtatggg catatgcatg aaattcagac cagttacata cactacaaca tggaaggagc
     4621 aaccctatgg ctaaaaaatt tggcaaattt ctgctgttta ccgcagcagt aggtaccgca
     4681 gccgcagctg cttactacta tatgcaacag aaagattctc agctgaagga atcttccgat
     4741 gcagatgatg attatgatga tttccacgag gatctggaca agagtaccga gttctcccgc
     4801 aattatgttc ctctgaatac acccgtgcag acctctgccc cgaaggaaga gacaggcaca
     4861 gagtccacac cggcttccga agaggcggca cccgcttccg cagaggcatc tgatttcaca
     4921 ccgctcaccg atcaggctac cggattgtcc ggcgaagagg gcgagaccgt cgaagacgtg
     4981 gaagagttct tcgatgaaga agatgccgag gacgaagagc ctcccgtaaa tgaagaataa
     5041 tcgttctatt tcgaaggtct cagagccatg ctttttgcaa gcgtctgaga ccttcctgca
     5101 tttccgcctc atcaatactt ccaaagccca acagcacggt agctttccgc attgtggctt
     5161 ccaccatatt ctctgacatc ccataggctt tcactttctc cgccgctgct ctctccaaca
     5221 gttcctgttc cgtgacattc cctttcgctg tcaacagaag atgcagtccc gcatcctccc
     5281 cggagatcac aaactgcttc ttaaacggtt ccagcccggc caacagcagg tcatgtttgg
     5341 cccggtagcg catccgcatt ttattcagat gccgctcgaa atacccgtcc cggataaatt
     5401 cattcaggat cctctggtcg attctggaca ccgtgcagga ataaaaagag caatccctgc
     5461 ggtagatatc cagcagatcc cccggcaata ccatatagct gacacggatt gccggtgcaa
     5521 tagccttgga aaatgtaccg atgtagatca cttttccctt ttcatcggaa gattgcaggg
     5581 aaggaatagg cttgccccga taccggaatt cactgtcgta atcgtcctcg atcagataac
     5641 gatccggctc cctttccgcc cagcgaagca gttctgctct cctgccgatg gtcatgaccg
     5701 caccggtggg atactggtgg gaaggcatca cataggccac tctgacaggc agtcccgaaa
     5761 gtctgtccgc ccgcattccc ttatcatcca tgtctaccgt gtgaatgcga taggcaaaag
     5821 attcaaagat gcggtatgcc cgcttgtacg tgggattctc catggcaatg cccacatgtc
     5881 tgcccagtat tttctccaat agcaacagca gataatcgtt tcctgctcct acaatgatct
     5941 gttccgggcg gcagttcacc ccccgggaag aatgcagata ccggctgatg gtcagccgca
     6001 gatcataatc tccctgaggg tctcgctgcg caaacaggac actgttgttg tcattccgaa
     6061 tattcttcgt gatccg
//
LOCUS       DAWDHD010000154         6076 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_753_length_6076_cov_8.317555, whole genome shotgun sequence.
ACCESSION   DAWDHD010000154 DAWDHD010000000
VERSION     DAWDHD010000154.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 6076)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6076)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6076
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_753_length_6076_cov_8.317555"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1679 a         1265 c         1756 g         1376 t
ORIGIN      
        1 ctgctacatc gagcaggacg gaaaatatct gatgcttcac cgggtgaaga aggaaaatga
       61 cattaacaaa gacaaatgga tcggcgtggg tggtcatttc gaggcggacg aaagcccgga
      121 ggactgtgtg ctccgggaag taaaggaaga gacagggtat actctgacgg cctaccggtt
      181 ccggggaatc atcacctttt tataccggga cgtctgtgag tatatgtgcc tgtatacggc
      241 ggatggattt acgggggaac cgagggagtg tgacgaagga gcactggaat gggtggagat
      301 caataaggtg gaggaactgg atctctggga aggggacaag atctttttcc ggctgcttcg
      361 tgagaaccgc ccctttttct ccttaaaact gtcttacgat gagaatggta tccttctgga
      421 tgcagtactg gacggtgagc cgatagcgac aaaggaaggc agaaaaatgc cggaggcgga
      481 aagtgggaca atgaattgaa ttttacggaa tttgtacgga actatcacat acagttaaac
      541 gaacagcaga tgcaggcggt acaggctacg gaaggtccgg tactgcttct tgcggttcca
      601 ggaagcggta agaccaccgt actggtcagc agactgggct atatgatcta ttgcctggga
      661 ataacaccgg agtcgatcct gacagtgacc tatacggtgg cggcaacgaa tgatatgcgc
      721 aggcggtttg cggggatgtt cggagaagcg gatgcagagc ggctggagtt tcgcaccatc
      781 aacggcatct cccagaaggt actgcaatat tttgcctacc gtaccgggaa aacgcctttt
      841 caggtggcag ataaggaagc ctcggcggcc gtcaaacagt cttttctgga agtaacgggg
      901 aaatacgcca cggaaaatga tatcaaagag acccagctgg atatcaccta tgcgaaaaat
      961 atgcgtctga caccggagaa aatccgggat atggacacgg atgtggagaa attcccggag
     1021 atattccgta cttataatgc gaaactcagg cagatgcaga tgatcgatta cgatgatcag
     1081 atgatctacg ccctcagaat tctggaacag tatcctgagg tacttgcaca tttcagggag
     1141 cagtatcggt atttctgtgt ggatgaagcg caggatactt cgaaaataca gcatgatatg
     1201 atcgatctgc tggcggcgca gagccggaat ctgtttatgg tgggagatga agaccagagt
     1261 atctacggct tccgggcagc ttacccgcag gcacttgtct cctttgagga cagacacacg
     1321 ggagcaggga tccttctcat ggagaccaat taccgcagca ggcaggagat cgtccgggcg
     1381 gcggatactc tgatccagaa gaataaaaac agacatccga agaagatgac cgcggccaga
     1441 gaaggtggtg gctgcgttac agagatcaag gcagtatcca gacagggaca gtacaattat
     1501 ctgctgaaag tagcccagga ctgtgaacag gagacggcag tattataccg gaataacgaa
     1561 agtgcacttc ccatcattga tctgttggaa cgtaaaggat tgtcctgccg ggtgaaaaat
     1621 tcggatatga ccttcttttc ccatccggtg gtcaacgata tctgtgattt tattcagttg
     1681 tctctggatc catgggatgg ggagacattt cttcggatct attacaaaat gggagccggt
     1741 atcagcaaaa aggcggccat ggaggcggtg aatgccaata ccagacgact gacactgctg
     1801 gatacggtgg cggaaatgga tgaggtatcc gtttacacca gaaaacagtg caaggcactg
     1861 gctacccatc tggacaatat gcggtatgag tcagcaggaa aagctatcta ccggatcctg
     1921 aattatatgg gatatcagga attcatggat acccacgggt tggacagtgg caaggcggag
     1981 atattaaagg ccctgggaga tcaggagaac agcctgtttg atttccctgc aagattacag
     2041 aagctgcagg agatgatgcg agagggctac ggggatccga agagtaattt tatcctgtcc
     2101 acgatccatt ccagcaaggg actggaatac acaagagtgt atctggcgga tatgctggcg
     2161 ggagtgatcc cttcccagat tccggggagg ggctgtgtac cggattcccc ggaggcaaat
     2221 gcctatgagg aagagagaag gctttattat gtgggaatga cgagagcgaa ggaacagctg
     2281 tatgtattta cctttggacc gaatctgacc tctgcttttt caggagaagt gtttgagaaa
     2341 acgaagccgt taaacgctgt aagaaagacc ggtacggctg tcgggatcag aggctcgctg
     2401 ctaaagacac cggacaaaaa ggtgcagaag ctgccgccac gggaagtgga ggcctgcatc
     2461 gcagcctgtg agtccggacg ggaagtacag catcagaaat acggaaccgg caggattgcg
     2521 tccgtatcgg ctgacggcat tgcccagatt ctgtttgcag gcgaaaacag taccagaaaa
     2581 atatcccttc ccattgcgtt ttccgggaag atacttacct taaaataata cataccagag
     2641 agggacagcg atgtatacaa tcgtagtggc agatgacgaa gaggaattac gcagagcaat
     2701 cataaggaag atcgactggg agagcatcgg atttcaagtc gtgggggaag cggagaacgg
     2761 catcgaagct ttggaactgg tggagaggat ggaaccggat ctgctgctta cggatatccg
     2821 gatgcctttt gtctccggaa tcgagctggc aagacaggtc agagaggtac gtcctaccac
     2881 acagatcgcc ttcttaagcg gcttcgatga attttcttat gcccagcagg ccatccagta
     2941 caatatcatc agttatatgt tgaaacctat cactatggtg gatctgacgg aagaactgaa
     3001 gaagatcaag cttaagatcg acggaatctt tgcggaattt gatgccagaa aacatgataa
     3061 tccggatctg caacagtttt ttctgcccct tttgctagat gattatgcca gctttgaggg
     3121 gcaggaccgg gaggaacttt tacaggaaca ggcggttggc tacggctttt tgaaggatcg
     3181 taataatgcc tttcagtatg cggtgctggc catcacggtg gaggatacca acgggaagaa
     3241 ccgtacgaga ccggaactgg tacattccgt ggatatgata ctgcaaaaat atatgaagca
     3301 ttattctttt gccatggacg gcaggatcat tgcagtgctg gcggctacca cgtcgacctt
     3361 cgaccggtat ctgcatattg ccgtgggaga gatggtacag agtacggaac gcattctgaa
     3421 aatgcattgt catatcggtg tcagccgtat cagagaacat ctgggagaat gccacgaggc
     3481 ctacagagga gctatgagtg cattgggata ttgcactccg ggagagagcg gcatccatta
     3541 tattgccgat gaagagcggg ataacaccat ggacatggag ctggcggcgg aatgtgttac
     3601 tcagatggag aatctgatcc gtggtggttc ccgagaggaa ctggaaaact atctgaagga
     3661 tgtattcggc aaactggagg tgcagcagga tgctgccctg aaatgcaaat tcctgatctt
     3721 ccggatgtcc tcggcgctga tccgcattgc ctatacggtg acagaagatt ccgccctgct
     3781 ccagcgtttt gccatgctgg aaggtccgat acgggaatcc agggatcgtt tcacggcact
     3841 ttgcctggaa gtatgggaac tggtcaatgc gggcaagaaa aagagcagcc agatcctgtg
     3901 tgaaaaggcc atgcagatca tcgacaagga gtatggggat ccgtccctgt ctctggtggg
     3961 cgtcagcaac agcattcatg tgagccccaa ttacctgagt gccctgatca aaaaagagac
     4021 cgggtcaagc ttcgtggatc tgttgacgaa aaagaggatc gaaacggcaa gggaactggt
     4081 actgggcagt tccatgaaga tgcgggagat tgcggagcgc tgcggttaca gcgatcagca
     4141 ttatttcagc tactgcttta aaaagtattg cggggtatct cccaatgcca tgcgacagga
     4201 gaaagaataa gatatgaaga agcaaaaaac gtccctttct tttatcatta cgacattagt
     4261 ggtgggactg gttaccctgt ttttgttgtg gtccactttt tacttttttt ctctttatgt
     4321 cggaagtatg aaacaggcgg cacataccag cagtgaacag gcggtgactc aggcttccaa
     4381 taccatttct aactacatcg gagatatgca ggagatcatg agtctgatcg agaaggatct
     4441 gacaaagagc ggcggaagta ctgggcagac actggagact ctttgcagtg tccggtcgga
     4501 tctggtggcg gtctatatct atgatgagca ggggaagctg gtggattccc atacgggaac
     4561 tcatactctg aaggaacatt atctgaagga tctgtcttat atcgaactgg attcctacag
     4621 ccccggggta ctctatctgt cggaacccca tgtggaatct ctgttgcagg actattatcc
     4681 ctgggtggta tcggtcttac aggagattcc cggagcagac ggacagaagc tgcgggtagt
     4741 cattgatatc cgtttctcca aaatatcgga ttatgtggac gatgtgggaa tcggacagca
     4801 tggctactgc tttatcacag acagtaatgg agagatcatc tatcatcccc aacagcagct
     4861 gctgtattcg ggtcttaaga cggaagccac ggagggactg gatctgagcc acaacggttc
     4921 ctttgagacc gaagaactga tctgtagtgt cctggcgctg gataactgta actggaaggt
     4981 ggtgggtgtc agctatatca gtgaactggt gacggccaaa gagatggaac tggtgggaaa
     5041 catctgtgtc atgctgacaa tcattctgct gacgaccatg gtggtcagtg ttctggtatc
     5101 cagactggtg accaatccgg tgcagcgtct gatccgggct atggaagagt tcgaaaagga
     5161 tgctgcggga tatgtttatc agcctgtgga aggcaccacg gagatcgagg cactgtcaca
     5221 gtcctacgag cacatggtaa gaaaaatcca gaaactgatg aagcaggtac gtcaggaaga
     5281 gatttccctg cgcaaaacgg agttaaaggc tctgcaggcc cagatcaatc cccacttctt
     5341 atataatacg ttggatgcca tcgggtggct ctgcgaggaa gaaaggtgtc aggatgccgt
     5401 ggagatggtc aatgctatgg cgaaactgtt tcgtatcagt atcagcaagg ggcacgaatt
     5461 gatccccatt gaaaaagaag tagagcatgc cagaagctat ttaaagatcc agaatttccg
     5521 ctataagaat cagttttcct atgagtttca ggtggaagag gactgtctgc aatattactg
     5581 taacaagatc accctgcagc ccattatcga aaatgccatc tatcacggac tggaccgcat
     5641 ggtggatgaa ggacggattc tgatccgcat ctacagcgaa ggggaagatg tggtcttccg
     5701 ggtggaggat aacggtgtcg gtatgtcaga ggaacagtgt cggtcgatcc ttcataagga
     5761 gccgggagat aattctggca tcggcatcaa gaacgtcaac gacaggatta agatttattt
     5821 cggaaaagag tacggtcttt ccatagaaag cgaactggat gaaggcacta ctgtgatcat
     5881 acggatgccg aaggtggaag aaaatgcagc ggcaggatgg gagaaagtat gagaaaacag
     5941 aaaatgaaaa aaatactgtc cctgctggct gcgggtgtcc tgcttcttat gagcatgagt
     6001 ggctgcggaa aagaagctga gacagggaaa acccaggtgg ctatgatcgt gaagtccacg
     6061 gagagcgcct ttttta
//
LOCUS       DAWDHD010000155         5961 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_767_length_5961_cov_10.814257, whole genome shotgun sequence.
ACCESSION   DAWDHD010000155 DAWDHD010000000
VERSION     DAWDHD010000155.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5961)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5961)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5961
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_767_length_5961_cov_10.814257"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1601 a         1191 c         1749 g         1420 t
ORIGIN      
        1 atcatccgtg cggagaaaca actgtccgac aaggaaccaa gcggggctgt atcaccggac
       61 accctcacgg cggatatgct ggacgcttat ctggataccg ctgggatacc ggatccggat
      121 ctgctgatcc gtacctgcaa tgagcagagg atttccaatt tcctgctgtg gcagctggct
      181 tatacggaat tttactttac tccggttccc tggccggatt ttacaaaaga agaattgatc
      241 aaggctgtgg aagcatataa tcatagagac agaagatatg gcggcctgaa ggaggaatag
      301 cttatgtttt ggacccgttt gctcagtggc atcgtattgc ttgccattgc actggctacg
      361 ttcagcttcg gcaatgtatt tctggcagtg ccgttgtggc ttatttctct gattgcatac
      421 agagagctta ccaaggcatt aaaatgtgca acggatgaga aaaaatttaa tgcactggaa
      481 tggatcggtt ttgtgggagt gacggcatat tatgcaacac tgtttttcac cagagaccat
      541 acattactgc tgatgtgtat tgtagcactg ttcatggcgg aaatgttctg ttatgtggaa
      601 gcctttccga aatatcaggc aagtcaggtt atggcagcag tgttctcttt cctgtatgca
      661 ccggtgctgt tgtcctttgt ctatctgacc cgcgagtgtg agaccggaat ctatctggta
      721 tggctgatcc tgatcagttc atggggatgc gatacctgtg cctatgtcgt aggcaaactg
      781 atcggaaaga agcatatttt cccggaatta agcccgcata agtctctcga gggatgtatc
      841 ggcggtgtcg ccggagcagc cctgatcggt ggtttgtatg cacatttctt tgtggaagcg
      901 gcattccctg atcagatcat tacctggaca atcgcattca tctgtgcggc gggcgcggtg
      961 atgagtatgg tcggtgactt ggcggcatct gccatcaaga ggaaccataa catcaaggat
     1021 tatggcaaac tgatccccgg acatggtggg atcatggacc ggtttgacag catgacggtg
     1081 acagcaccga tgacgtattt tcttgccatt ctgatgattg gactgcgtta gtcccaaagg
     1141 taagatacaa ggagcatagc actatgaaaa aaatagcaat tctggggtct accggctcca
     1201 tcggaaccca gactctggaa gtcgttcgca ataatccgga attgcaggtg gtagcgctgg
     1261 ctgccggaaa aagcgtagaa cagatggaac agcagatcag agaattccat cctctgatcg
     1321 caggcatgtg gagtgaggaa gcggcagcag acctgcggag ccgtgtggcg gatttacctg
     1381 tgaaggttgt aagtggcatg gacggtctgt tggagatcgc aacaatgcct cagtcccagg
     1441 tattagtgac ggcgattgtg ggaatgattg gtatcagacc gacgatcgcg gccattgaag
     1501 cagggaaaga tattgccctg gctaacaagg agacgctggt aacggcaggt catatcatta
     1561 tgccgttagc ggcgaaaatg ggtgtgaaga tcctgccggt ggacagtgaa catagcgcga
     1621 ttttccagtc actgaacgga gaaccggccg gaaggattga gaagattctg cttacggcat
     1681 ctggtggtcc gttccgcggc aggaccagag agcagttgca aaatattcag gtggaggatg
     1741 ctttgaagca tcccaactgg tccatgggac ggaagatcac cattgattct tccactctgg
     1801 tgaacaaagg cctggaagtc atggaagtaa agtggctgtt cggtgtggat ctggaccaga
     1861 tccaggtcat tgtgcatccc cagagcatta tccattccgc agtacagtat gtggacggag
     1921 cggtgatcgc acagttggga actccggata tgaaactgcc gatccagtat gcgctgtttt
     1981 atccggatcg gcgtccaatg ccgggaaaac gtctggactt ctatgaactg gcacagatca
     2041 catttgaaaa gccggatatg gagacattct ttggcctgaa gctggcttat gatgcacagc
     2101 gcatcggcgg cagtatgcct acggtgtata atgcagccaa cgagaaagca gtaggactgt
     2161 tcttagaccg taagatcgct tatcttcaga tcccggagct gatccgtgag gccatggagc
     2221 agcataaggt gatcgagaat ccgaatgtgg aggagatcct ggagacagag gcatctgtct
     2281 atgattttat tgaaaaagtg tattctgaga ggcaataaat ggttactttg atcttgtttg
     2341 tattgatctt tggggtggtg gtaatctcac atgaattcgg acattttctg ctggcgaagg
     2401 ccaatggaat tcatgtgata gaattttccg tgggtatggg cccgaatctg ttttcttttc
     2461 agaaaggaga cacgaaatat tccctgaagc ttcttcccat cggcggagcc tgtatgtttg
     2521 agggggaaga cggtctgaat gagaaggagg acggtgaaga tcacagcgga tctttcctga
     2581 acgccaatgt gtgggcgagg atttccaccg tgttggcagg acccatattt aactttattc
     2641 tgggctttat cattgccttt attatggtga atctgattgt gatccgtgat ccggtagcca
     2701 cggagatcgt ggatggcggc gccgcacagg aagcgggact ggaggccgga gacaggatac
     2761 tttccttaaa tggcagtaaa atacacctgt atgaagaaat acagttattt actctgactt
     2821 accgtggcgg agaagtagcg gtggaatatg aaagggatgg tgtaaaggga actacaacgc
     2881 tgacaccgaa gtacgatgag agcgaaggcc gctatctgat cggcatcagt aatgcggatt
     2941 tcgtagaact gtccggattg gacagcttcc ggtattcctg gtatgagatg cgctactgcg
     3001 tgaacatgac ctggaagagt ctggcgatgc tggtgcaggg gcaggtctcc aggcaggatg
     3061 tggcaggccc tgtaggcatt gcggtaaatg ttgttggcaa gacctacgaa agtaccaagg
     3121 attacggctg gcagaatgta ctggtgaata tgttgaacat caccctgatg cttactgtta
     3181 acctgggaat tctgaatctt ctgccgatcc ccgcgctgga cggcggccga ctggtattcc
     3241 tgatctggga agccatcaca cacaagccgg tgcctcccga aaaagaaggc atggtacact
     3301 ttattggact gatgttcttc atggtactga tggtgttcct gttatttaat gatctggtta
     3361 atatcttcgg ataagccgga taattcaaga cgttcaataa gtaagatccg ttgacaaagg
     3421 gcataccgca tggtatgctc tttttatatt tgtgcaaggc acatttcgtt gaaaaatttc
     3481 atgccgatcg gcaaaaattt cctgatgtag caatgtaaca ctgaagtaag aaacagtacg
     3541 gacaaaacag aataaaaaag gaggaatgtg tatggagcat gtgacagcct gttattggca
     3601 aaaggaaaca cctgcagagc tgtttttatc tttgcagcag cgaaaatacc gtaggctggg
     3661 gatttccgtg gtatgcgcct gtatctcgga caacggggaa atggtaagaa gtctgcaaaa
     3721 caaaatggag gaggaactgg aagaagagaa tgtctggagg ccgtttcggg aagaaatatt
     3781 gcaggagaaa tggatggaca ttataaggca gcaaaagcag gatgacagtt atgcgggaat
     3841 cttatgcgtg gggagcagag tattgttttt cagtcacggg agaatgcgga tctgtggatg
     3901 ttttcagaga ttcggcagga cacaatggag gacgatgcag gaatcctgca tggcgggaga
     3961 agtggaaccg gggacagcca tgttgatggc ggacaatggg ttcctgaatt tctgtgaaga
     4021 ggaactgaca gcatgtctgc aaccgcagat tgcaggaaga ccggatgcac gggaacgcgt
     4081 ggaaggcgct gcaagacggc tgaaggaact gggagaaaag gcggagagac acggaggaac
     4141 acacatggga gcggtgtgga tcctgccggt tatgggagga aaaggatgga gcagagaata
     4201 acagaaaata tgggcagatc actaaaagaa catggctatc tggatccggt atttgtgggc
     4261 aaggggagtt tcgcgaaggt ttaccgcgtc cgggacgaaa aaagggactt tcaggcatgt
     4321 aagatatcga aagttacagg acagtgggaa caggagtgtc gtaacagcag agaaatctgt
     4381 catcctctgt ttccggccta cagagaacac tggacagacg gggaatgggg gtatctggtc
     4441 atggaattct gggatggctg tgacttgcgg aaaatgctgg acaggagggg gagactgtca
     4501 ccgggacagg cggcccggat agctttgcag attacagagg gattgcaata tctgcatgaa
     4561 cggccgcatc cgtttctcta ccgggatctg aagccggaaa atatccgcat ccgcgtggat
     4621 ggcagagcgg ggattctgga tctgggatgt ctgtggaacc gggagcagga ctggtcggga
     4681 gcgggaaacc gaagcttcag cgctccggag cagttcgtgc cgggggagat tcccggggaa
     4741 gagagtgatg tctatgcagt gggcagactg ctggcagtta tgttgggaga tgacacagac
     4801 ggcacagtca gacaatctgg gggaataagg aggcgtaatg cggaaaaacg ttgcggacga
     4861 aaagagcaaa gacaggaaaa atggcttcgc aaagtgatcg ccatggcaac ctgtgaagag
     4921 cgtaaggatc gtatcccgga gatcaaaatg ctgcgtactc ttttgatggt gacggatgat
     4981 tccgggaggg agaggaaacg tgcatgtagg gcagctgatt tttactatgt ccggaagttg
     5041 tactgcccat agttgtacat tccgtgctcg aacacaatca agaaaattta caaaaaagtg
     5101 cttgctgttt tggagagaaa tgagtatact gtaactaaag gttcaaagaa gcgtatgagg
     5161 accggaagca attaaattta catagcaaaa aggagaagag ttatgcgttt ttttattgac
     5221 actgcaaaag tagaagacat taagaaagca aatgacatgg gggttatttg cggagtaact
     5281 accaatccct ccctgatcgc aaaagagggc agagatttcg tagaagtgat caaagagatt
     5341 gcatccatcg tggacggacc tatcagcggt gaggtaaagg ctaccaccgt agatgcagag
     5401 ggtatgatca aagaaggaag agagattgca gcgatccatc cgaatatggt cgttaagatc
     5461 cctatgactg tagaaggttt aaaagcatgt aaggtattaa gctctgaggg aatcaagacc
     5521 aatgtgaccc tgatcttctc tgcgaaccag gctctgttgg ctgcccgcgc aggtgctacc
     5581 tatgtatctc ctttcctggg cagactggat gatatcagca ccagaggtgt agatctgatc
     5641 cgtgatatcg ctgatatttt cgcagtgacc gatctggata cccagatcat cgcagcaagc
     5701 gtgcgtaatc ccatccatgt aaccgattgt gcactggcag gtgcggatat tgctaccgtt
     5761 ccttactccg ttatcgagca gatggtaaag catcctctga ctgatgccgg tatcgccaag
     5821 ttccaggcag attataaggc agtctttggt gaatagtctg tcaatgcata ggcgggacga
     5881 ctgcttgcag gcaggaacgc ctatgcatta gacggacaaa cagacatgag caagtagggc
     5941 gacagcccgg aatgcgaatg g
//
LOCUS       DAWDHD010000156         5946 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_771_length_5946_cov_10.651163, whole genome shotgun sequence.
ACCESSION   DAWDHD010000156 DAWDHD010000000
VERSION     DAWDHD010000156.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5946)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5946)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5946
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_771_length_5946_cov_10.651163"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1569 a         1244 c         1735 g         1398 t
ORIGIN      
        1 tgggcgcatt gctgtgttct tcgttatatt cttttaactg ctgcgccaga ctttctatga
       61 aatagtccat ggtatcatgg gaaccgtggt tgacgatcat gaggaattca ttaccgccgt
      121 tacgtcccac tacaccgaag gcatcgccaa cgctctccag aatgccggag aaatctttta
      181 acatctggtc gcctaccaca tgtcccatag tctggttgat ctccttcagg ttggtgatgg
      241 tcaccatata gcaggctaca tgagccagag attccggaga gtcataggtc tcgaagacta
      301 cgtccagacc gtgccggttg gggataccgg tcagatgatc caggaaggcc gcacggttca
      361 gagcatggct ttcttcggca aaagtgcgta atttatagaa atcgtataat aaccatatca
      421 gattaaataa tgcaaggatc caggaaagtg tgcataagat aaacagagtc cggtcttcga
      481 aaagctttgt gcccagtgca gggttcagag tcaggaccag aacataggcg gtttccaaca
      541 gcagaagaag aattaactgc acggttttgg tgttacggaa ggtttttacg aatttctttt
      601 tctgtatcat ataggttcac tcctttgatt gcgctggtgg tgaggtgtac atcgggaaat
      661 cacttcgccg ttcgccaatc atgcaagcat gttggctctc ttgcgctcat tatatcataa
      721 caggcactta aaaaaaagaa aagaaatatt gagccattcc gcttcttagc gccgtcttta
      781 tatacggaag tgcttccgga gtgtatgagc ggaggcgtgg ctgcatatat ggaacgtatt
      841 atttatacgg tttacataag aagaaaacgg acaggccaat gtccggcgta agatatccat
      901 cagggaattc cctggtggat attttcattt tagggaaaag cagaatttgt cgaaaaggag
      961 aggacaatgt tggaaacgat cttgaataat tttcatctgg agaagatcct gtggatcctg
     1021 caaaaaagga ttgcatacat tatgatcctg ggggtactgg gaggaatggc aggaggcgct
     1081 tatgcatatc tgaccaacag tatactttac cgggcggagg tatccttcta tgtatacagt
     1141 gatccggact acgtgtatga ttcttcggtc aatatctcaa attccgaatt tacccaggcc
     1201 aagaatctgg tacagagtta catattaatc ctgaagagta acacgatcct tcagaaggtg
     1261 ctggaggaag ccgggctgga ttacggaacg gaagctttgt cgggaagaat cggtaccagt
     1321 gtggtggaaa acacggcagt gttctatgtc tacacttatg acagcgatcc ttaccgggcc
     1381 atggagctgg ccaatgctat cggcagggtg gctccgaagg agatcggccg gatcgtgaag
     1441 tccggtggca ttgaggtgat cgattatgcc acactgccgg aagcacccta cagttccacc
     1501 agtctggtca attatgcact ggtgggcttt gccggatgct ttggggcatc ctttgtattg
     1561 tttctgctgg caggactgat ggataccacg atccgcaggc ggtacgaact gaagctggca
     1621 ttccggattc cggtgctggg agatattccg cagatccagg cgccggaccg gaagagtaag
     1681 cccgtgttgg tattggggga gaacagctcc tttgccgtga aggaatccta taataccatc
     1741 cgggcaaacc tgctgttcac gggaaaagga gaacgttgtc cggtctatgc cgtcaccagt
     1801 gcggataagg atgaaggcaa gactttgaac agtgtcaaca tcgccatcag ttacgcacag
     1861 ctaggcaaaa aagtattgct catcgacgga gacatgcgga atatgtcggt ggcatctctg
     1921 ctaaagctcc ggggcagact tggactgagc cagtatctgg cgggattgcg ggagacaccg
     1981 cagatcgtgg aataccggca gaatctggat gtgctggtgg gaggagaaaa tccaccgaat
     2041 ccttccgaac tgttgggcgg agagcggatg aaggagctgc ttacgacgtt gcgtagccgg
     2101 tacgactgca tcattattga tctgccgccg gtaggaattg tgacggatgc cctgctattg
     2161 tcccatgaag tcaccgccta tcttctggtg gtgcgggccg gcgtcagcca tatgagccgg
     2221 gaaaagtcgg cggtgacact gttagagcag gtggatgcgg atatctgcgg catcctgttc
     2281 aacggcctgc cgccaaaatc taaggactgt aactacagac tgcagcaata ctggcaggaa
     2341 tataaacagc aaaatgggga agcagtttga aaaaaacagg gaggtcttta tgaaaatatg
     2401 ttttgccgca tcgtcgggcg gccactttga acaactgatg atgttaaaac cgctaatgga
     2461 aaaatacgaa agttttgtag taacggagaa aacaggctat gaggtgaaga caccggtgaa
     2521 atgttactat ctgcagcagg tgaaccggag acagtggaga tgcctttttt atctgttgaa
     2581 aaataccgtg gattcgttgc ggctgttgca taaagaacag ccggatgtgg tggtctgcac
     2641 cggagtgctg gccatgatcc cgctgtgcat ggaagccaag ctctccggca ggaagctgat
     2701 ctacatcgag tcatttgcca aagtgacttc ggccacactg acgggaaaac tgttatatca
     2761 ttatgcggat cgtttttatg tccaatggac acagatgaag gaaatctatc cggatgcgat
     2821 ctgcctgggg ggaatttatt aggatgatat ttgtaacgac cggatctcag aagttccagt
     2881 tcaatcgatt gttacaaaag atagatctgc tgatcgagga aggcatgata agagaggagg
     2941 tgtttgccca gatcggagtc agcgattatc agccaaagca ttatgaagac tgctgggttc
     3001 tggacagaga aggcttcacc gggaagctgc gggaatgccg gatggtcatt acccatggtg
     3061 gaaccggtgt gatcatcggc gccgtaaaac aagggaaaaa agtgatcgca gtgccgagac
     3121 ttgccagcta cggggagcat gtggacgatc atcaggtaca gctgcttcgg gaattcgacg
     3181 gaatgaacct gatctgtgcc tgttatgaca gtgagaaact gggggattgc atccgggata
     3241 ccggcagcag ggactttgcc ggataccatt ccaatacccg gaggatcctt aaggatattg
     3301 agaatttttt aattgcggaa gagggggaaa accatggaaa acattcaaaa atttcgtaaa
     3361 aaagggaaaa aactgggaga gtatatgatg acgaagctgc gccttttgcg ggatcagaaa
     3421 aaatatcgct cccggggagt aacattaaaa tatatgctgg accgggtgga ttcccgggat
     3481 ctgatcatcg ttttcagctc ctgcaccaga aaagggatcc gggccagata caattatgtc
     3541 cggacgttaa aggaagttcc ctgtaacaag ttgtttctgt tggatgattt tgcgtcagat
     3601 cacagaggtt ccttttatct gggcagcaat atgaaattcg aggaggcggt cgttgtaagg
     3661 gaactgatcg accgggtacg ggagcagacc ggagcaaaac gcctgatctt ctgcggttcc
     3721 agcaagggcg gttacacggc actgaatttc ggactggact atcccggaag ctatatggtg
     3781 gtgggggggc cccagtattt tctgggacaa agcctgctgg acaccgggaa tattgaggcg
     3841 ttgaagcata ttgccggaca ggtctgtaag gaaaaggtcg aattcctcaa tgagtatctt
     3901 ccgaaaaagg tggcagccaa ccgttatgcg ccctcccaga agatctatct gcattattcc
     3961 gacagcgaac acacctatga ggaacatatc cgctatctgc gccgggatct ggcgaaaaag
     4021 ggataccgct gcagcgagga cgtggcacat tacaaggaac acggggagat cagtctgtat
     4081 tttccggaat ttttattaca tagtgtggag gaaatcttat gccggcagtc agcgtgatcg
     4141 ttccggtata caatacggaa aaatatctgg aggaatgcat tcgttccctg acagagcaga
     4201 cgttgtcttc actggaactg atcttcgtgg atgacggttc cacggatgct tctcccaaga
     4261 ttctggaaag atatcgtgcc agggatcccg aacacatccg ggtcatcacc cagaagaacg
     4321 cagggcaggg aaaggcaaga aatgtaggac ttgcggctgc cacgggagaa tatgtgggat
     4381 tcgtggattc ggatgattat gtggacaggc gtatgtacgc ggccatgtac accagggccg
     4441 gttccgaaga actggatctg gtggaatgtg attttcatta tctggaggaa gacggcagag
     4501 agaagcgctc ttacggttcg gcggctccct ggaacaagct gatccgcagg agactgctta
     4561 cggacaacga tatatggttc ccggaggggt atatctatga ggacacctct ttctacctga
     4621 agttacgccc ctttgtgaaa aaacaggcga aaatgctggg agtctatgtc tatcaccggg
     4681 atagagggga ttctaccatg aatgacaata aaagcagacg ggtgggaaat atttttccgg
     4741 tattgcagga tgcctgtcgg ttttaccggg aaaaagaact gatggatact tatgggaaag
     4801 aactggaata tttctgtacg aggatcctgt tgtgcagttc catggggcgg atctccagag
     4861 tgcgggaccg gaagctgcgg aaatctctgc gggaagagac ttttgtgttc cttcgggaaa
     4921 attttccgga gtatcggaag aacccctatc tgggcaatgg actgaaaggg cgttatctgc
     4981 ggatcgccac accggcattg tgctggatct gtgggggaac catcggcaga tgcaggccgg
     5041 gagggttacc gggatagaaa atggcggaca gagaaaaggg gaaaaatatg cggatatcgg
     5101 tagcgatggc cacttataac ggggccagat ttatcacaga acagctggat tccatccggc
     5161 ttcagagcct tccggtggat caggtgatcc tgcgggatga cggttcctcg gatcagacgc
     5221 tggagatcgt acgggaatat ctggagactt acgagctggc accggcatgg aggatcacac
     5281 agaacggaaa acgactgggg tatgcggaga atttccgggc agcggtggaa gagacggacg
     5341 gtgattatgt ctttttctgc gatcaggacg atatctggga accggaccgg gtggaagata
     5401 tggtgagcga gatggaaaaa cggagagaga tactggtgct gggatcggaa ttcgtaccgt
     5461 ttacagtgga tcccgatgct ccttctgtca gccggagcgt attgaaaagc ttccgggggg
     5521 acggaagcat ggagcatgtt gcatggagtc cggcccatat tttcatcggc agtgagggct
     5581 gtaccatgtg tctgcgcaga gagttcctgg aagagacgaa gaagtactgg tatcccgggt
     5641 gggcgcatga tgaatatgcc tggaagttgt ccctgtgcat ggacggctgt tacattctgc
     5701 acagtgttac gttacggcgg cgaatgcact cgggtaatgt atccaaacgc aagatgcggg
     5761 atctggacag acggatcgct tttttccggg aattgcagaa aagtcatgaa accactctgc
     5821 ggttcgccga ggactttgga atgccggagg aagcgaagga actgttaaga cgcaatatcc
     5881 gggccaccac ccttcggatc gagctgaggg atcagcgaat tttgaggagg atggggccgc
     5941 tgggat
//
LOCUS       DAWDHD010000157         5941 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_772_length_5941_cov_10.337920, whole genome shotgun sequence.
ACCESSION   DAWDHD010000157 DAWDHD010000000
VERSION     DAWDHD010000157.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5941)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5941)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5941
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_772_length_5941_cov_10.337920"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3169..3268
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1673 a         1309 c         1453 g         1406 t
ORIGIN      
        1 gcgagcgtgc cgcgtttctt ataccctgcc aagggctacg gggatatttc gctaactttc
       61 aatttacttg tcttgtgcaa ctgttcatat cgcaaagttc tgacaactta caaaaatatg
      121 aaataattaa caatatttac ttgtaatagt aacatatatt tagtaaaata cacgtaaggg
      181 gttaaaaatc caatccaacg gctggacaaa atacccaaaa tgcagaataa atggaggatg
      241 ggatatgaat atttacacaa cagacaagat ccgtaacgta gtattactgg gccatggcgg
      301 cagtggcaag accagtcttg cagaagcctt tggttatctg tccggtatca cctcccgtat
      361 gggcaaggta gatgacggca ataccctcag tgattacagt aaagaagaac agaaacgtca
      421 tttctctatc agtacctccg tgatccccat tgagtgggaa ggatacaaga tcaatattat
      481 tgatacaccg ggttattttg atttcgtggg agaagtggaa gaggcagtca gtgccgcagg
      541 agcagccatc atagtagtta acggcaagtc cggcatcgaa gtcggcaccc agaaggcatg
      601 ggagctttgc gagaaataca agctgccccg ttttatctat gtctccaaca tggatgtgga
      661 taatgcctcc ttccgtcagg tagtggaaga tatgactaat ctgtacggca agaagatggc
      721 accgtttcat ctgccaatcc gtgagaacga gaaattcgta ggatacatca atgttatcac
      781 cgagagcggt aaccgctggg agggtaagga agttgttccc tgtgacgtac cggattacag
      841 ccgggcgaat ctgcagatct gccgtgatac cttaatggaa gccgtagccg agaccagtga
      901 agaattcatg gagcggtatt tcggcggaga gaccttctct gaggcagaga tccgtgcagc
      961 actccgtacc aatgtctgcg atggcagcat cgttcccatg accatgggtt ccaacatcct
     1021 gtgtcagggt atgtacacac tgttagatga tattgtcaaa tacatgccca gccccgagaa
     1081 cagagaagtg gcgggcatca atttaaagac caatgagatt taccatgcgg attacgattt
     1141 cgccaaggca aaatccgcct atatctggaa gaccatagtg gatcctttca tcggaaaata
     1201 ctctctgatc aaggtaaact ccggtgtact taagaccgac gacctgctgt acaacgtaga
     1261 ccgtgacatt gaggaaaaga tcggcaaaat ctatgtactg cagggcaata agcccattga
     1321 agtcagcgag ctgcatgccg gagatatcgg agcactggcg aaactgaccg cagcgagaac
     1381 cggcaacagt ctctctacca aggccacgac catcaaatat ggcaagttcg atatttccac
     1441 tccttatacc tatatgcggt ataagccgaa gaataaggcg gacgtggata agatctccca
     1501 ggcattgcag aagatgaccc atgaggatct gaccatcaaa gtagtaaatg atgcagaaaa
     1561 ccgtcagacc ctgctctatg gtatgggcga ccagcatctg gatatcgtgg taagcagact
     1621 gctgaacgaa tacaaagtag agattgagct gtccagaccg aagatcgcat acaaagagac
     1681 tatcaagaaa cagtccgatg tggaatataa gtacaagaaa cagtccggtg gacacggcca
     1741 gtacggtcat gtaaaaatgc gtttttcacc ttccggcgat ctgaccaaac cctatgagtt
     1801 cgcaacagaa gtcgtaggcg gcgcggtacc gaagaatttc ttccctgcgg tggaaaaagg
     1861 tattcaggaa tccgtaggca gaggacctct ggcggcctac ccggtagtcg gtgtcaaagc
     1921 ggtactgtat gatggatcct atcatccggt agattcttcg gaaatggcat ttaagaccgc
     1981 agctatccag gctttcaaaa aaggtgtcat ggaagcaggc cccgttctgt tggagcccat
     2041 catgtcctta aaggttaccg tgccggatgc ctataccgga gacatcatgg gagatctgaa
     2101 caagagaaga ggccgggtgt tgggcatgac acccatgagc ggcggcaagc agatcatcga
     2161 agcggatatt cccatgagcg gattattcgg ttattgcaca gatctgcgtt ccatgaccgg
     2221 aggcagaggg gattattcct atgaattcgc tcgttacgag caggcacctt ccgacgtaca
     2281 ggagaaggaa gtagcggcca gagcagcaaa ggttgcggag aacaatgcag aagactaacg
     2341 agtaattaca taacatacac aacaaaaaga gaccggttgg ctttccggtc tctttttcgg
     2401 tattgtgcag taaatatcat cgatatcgct gcatcagatt gatcagtaat gtgatccatg
     2461 caaacatgat tcatcacctc tcgtatacag catatgcccc ggcagacgaa aatatgcaaa
     2521 aaaccatgcg gatagagccg cagtccttga cagtatcggg aaaacacgat aaaataatcg
     2581 atagtttttt aacgacttga gaacaaaaaa tttaaataga aagcttctca tagtttagga
     2641 ggaaataatt atggcagtac cttacaatca cagagaggta gagaaaaaat ggcagactgt
     2701 ctgggatgat gaaaaagcat tcaagacttc cgacgatttc tccaagccca aatattacgc
     2761 actggtagag ttcccttatc cttcaggaca gggattacat gtaggtcatc cgagacctta
     2821 tactgcactg gatatcgtag cacgtaagcg cagaatgcag ggctataatg tgctgtatcc
     2881 catgggatgg gatgcattcg gtctccctac cgagaactac gcgatcaaga atcatatcca
     2941 tcccaagatc gtaaccaaga acaacgtggc aagattcaag aatcagctgc attccttagg
     3001 ttattctttc gactgggaca gagaaatcaa caccacagat cccgagtatt atcactggac
     3061 ccagtggatc ttcttaaagc tgttcaaggc aggactggct tacaaaacgg agatgcctat
     3121 caactggtgt acctcctgta aggtaggtct ggcaaatgag gaagtagtnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnct gtctgtggca gaagccaaag agaagatcat
     3301 gaactatctg gaagagaagg gcatcggtaa ccgcaagacc aattataaat taagagactg
     3361 ggtattctcc agacagcgtt actggggtga gccgattcct attgtgcatt gcgataagtg
     3421 cggatatgtt cccattgatg agagcgaact gcctctgcag ttacccgatg tagagagcta
     3481 tatgcctacc gataccggag aatctcctct ggctgccatg accgactggg taaataccac
     3541 ttgtccctgc tgtggcggtc ctgccaagag agagaccgat accatgcctc agtgggcagg
     3601 ttcttcctgg tattacctgc gttataccga tcctcacaat gacaaggcac tggcaagtcc
     3661 tgaagcattg aagtactggc tgccggttga ctggtacaac ggcggtatgg aacataccac
     3721 actgcatctt ttgtactccc gtttctggca taagttcctg tatgatcagg gcgtagtacc
     3781 tactcccgaa ccttaccaga agagaaccag ccacggtatg atccttggct ccaacggcga
     3841 gaagatgtcc aagtcccgtg gcaatgtggt aaatccggac gatatcgtac aggattacgg
     3901 tgcagatact ctgcgtacct atgagatgtt catcggtgcc tttgatctga gcgcttcctg
     3961 gagtgaggaa ggcgtaaagg gatgccgcag attccttgag agagtctgga agttgcagga
     4021 tatcctgact gatgaagaag gctatagtgc agacttagag accaagatgc accagaccat
     4081 caaaaaggtc agcagcgatt ttgagaacct gaagtacaac accgccattg cggctatgat
     4141 gtccctgatc aacgagttct ataagaagaa cgccattacc agaggcgaat acaagacact
     4201 gcttactctg ctgaaccccg tagcacctca tattactgag gaattgtggc agactgccgg
     4261 attcgagggc agactgtatc aggcagcatg gcctgaattc gaggaagaga agaccgtaga
     4321 gagcgttgtt gagatcggtg ttcaggtcaa cggcaagatg cgtgtgacca tcaacgtttc
     4381 caaggatgag accagggaaa acgtcatcgc tatggcaaaa gaagctctgg gtgacaagct
     4441 gaccggcaac attgtaaaag aaatctatgt tcccggtaga atcgtcaaca tcgtggcaaa
     4501 atagttcagc catggacaat aagtgaaata aaatcatgct tgcatgattt tatgatcgaa
     4561 tatgtccatg aggtaaacgc cgtcttatga acaaaggtag tcacgtagtg atggaaagcg
     4621 tgataacgcg actttgcgaa tggcgttggc tgaacgttga ataaaataac tcttgacaga
     4681 cattaagtct gccgggagtt atttttttgt gatcggcttt tctccagaat attttcaatc
     4741 tgacgaagct ccgattccgt actgtatttt ttgatagcgg tgatggcagc ctgcatttcc
     4801 tgcggggtaa aagtcaggtg ctcctgtttc gctttacgca tggcgggcat gagaagtgcc
     4861 atcatttctt tttgactttt accgtgcccc tgggcaaaaa gtttcgagag aaaatccagt
     4921 ttttctctgg ggatgtcctt cacgagatca tcctgcatcc agaggggtgt cttggaatag
     4981 tccatttttc tgacctcctg tgtctgattt tgcagtggaa aaaagaccgg acaacataga
     5041 cggatccatg ccggagagat cggagaagcc ctccgggaac aattcctgca tggtctgcac
     5101 catttccatc atttcctgga aattatgaat attgtccatc atttgtatga tttgtcgaag
     5161 agctgatttc tgagtctcgt cgcacaaggg catgagatca ttacacagtt cagtggcagt
     5221 ttcttcgttt gcagaagagg gagcaatgca cagagaagtc tccggatgcc tgcgaaaaag
     5281 tgtaagcgtg tattgcaact ccgacatttt tatgtaaaca gcaagtcctt tcagcagccg
     5341 gggatccata taataagcca gtattttcag aatctgaatg cggttggaag tgaataatgt
     5401 gtcaaaggcc gccgccttgt cgattctctt atcatccatg taccgatacc ttcctgcaaa
     5461 agagttacta gtattttatg agagcggcag catgcttatg ccgatcaatc cttcggggta
     5521 cagaaaagtc cgtaccccgg agtgatcgtg cctacaaagc tccgggagat cccggaaagc
     5581 agtatcagag attagcaacc gcaaccgttg ttgttaccgc agccaccgcc gcagaagcag
     5641 gacagaagca ggatccagat cagccattca cagcagtcat tgccgccgca gccattgttg
     5701 ttgcccagca gtccgcagcc gttgttgtta ccgcagccac cgccgcagaa cagcaacagg
     5761 atgatccaga tgcagctgtt gccgaagata ccgttaccac agttgttatt gccgcaggaa
     5821 ttattgcagc cgcaatttgt agcagttaag tcactcattt ttagtgcctc cacattttat
     5881 ttatgctcta ttgtatgcgg gggagcctgt caatgtttcc ggaaaagact tgcaataatt
     5941 t
//
LOCUS       DAWDHD010000158         5919 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_777_length_5919_cov_13.041780, whole genome shotgun sequence.
ACCESSION   DAWDHD010000158 DAWDHD010000000
VERSION     DAWDHD010000158.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5919)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5919)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5919
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_777_length_5919_cov_13.041780"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1511 a         1515 c         1321 g         1572 t
ORIGIN      
        1 ggaagcctgt gtcagacctc tgatctgctt tctcatcttc tcactgatgg acaggcctgc
       61 tgcgtcatca gctgcacggt tgatcttata accggaagac agtttctcgg tggactttgc
      121 ctgagaagcg gtggtcagac ctaacattct gttagagttc atagctgtaa gattgtgttg
      181 tactaccata ataatttacc tccttgtatt actgcccaat tccttttgcg gcagtgtgcg
      241 tatctgtatt gttattgaga aatccttttc tcccagatac ttgtttttag ttttatagta
      301 accggagtag gagcttccct gtcgggccct gacagagccg ctcttcctcc ggttattacc
      361 atatgaaatt atttgtccgc atttcccttt gtgatgatca cgttggattt tttgcggtac
      421 ttctccgggt gctcttcttc cgcataatat ttaggaagct tcgctctctc ctccggatct
      481 gtgatctggt tttctaatac cgtgcttcgc acgatgttca cctctctcgg tgcatcgatc
      541 atgatacgga gatgattctt agttcctccc aggaacacga tcttgatatt gtcattgatc
      601 atcaggtatt cttccggact aacggtcaga cgcagcatat aataacctcc ttgctctgtt
      661 cagccttcct tgcctgctgt ttctcactga tgcaacattc tattatggaa tgttgcattt
      721 cccaaattca tttatcattt caaggaggcc actatgagca caacacaacc tatccgcagc
      781 aaacagcttt tacatgattt caaaaactac tatcttaccg aacagccgga attccggaat
      841 tacaccatta tcctgatcgg tctgaacacc gcactgcgca tcaacgacat ccttcatatc
      901 acctggggtg acgtctattg tggagaacgc agacgttttt acactcatct tctgatcaca
      961 gaaaagaaaa caggaaaaga gacccgaatc ctgatcaaca gagaacttca ggaaacatta
     1021 ctgtcctacc ggacaacaca gccttccata gccgacacag actacctctt ccccagcccc
     1081 agaaaaaccg gttccccact ctcccgatac caggcctacc gcattgtccg taaggctgct
     1141 gaagccgtag ggatcgaaga cgtgatccgg tgccattccc tgcggaagac cttcggctac
     1201 catgcatgga aaatgggaac tccgcccgca ttgttaatgg aaatttacaa tcattcttct
     1261 tttcagatca caaaacatta tctggggatt gaacaggatg acaaagatgc tgtatttcgt
     1321 gacatacagc tatgacatgc ggcctttata attgctataa tgatatagaa ataacgtatt
     1381 agcgattttt ataaaaaaga ctaaagtttt cacatggtca gccgaaatat gtagtgcaag
     1441 acaaaatgca acattccata atagaatgtt gcatttttct attttaatcc tttcatttct
     1501 gtggtattct tttaaagact gcatatcgct atttttcgac aaatactttc cttttttgaa
     1561 acatatacag tcagaatcag gagtacacat gaaaagcttt atatgtgaaa tgttctataa
     1621 gcagcgggcg gcattcgagc aaagctgcgc ttccaggggt aaagaatatc ctcttccgga
     1681 agacgttttc ctacaatcgg atattttcta tgacgagaag catgttccgg cacatcgtct
     1741 ggatgtctac cgtcccaggg gacgggacgg agagattctc ccggtcatcg tcaacgtaca
     1801 tggcggaggc ctcctcatag ggaacaagga attcaaccgt cccttttgtg cctccctttg
     1861 tgaggcgggg tatctggtat tcagcattga gtaccggctg ataccggact gtctcttcta
     1921 tgaccaacta actgacctgc atctggcact ggattttatc cgggatcatc tgatggaata
     1981 cggcggtgac atttctcaca tatatgcctg cggcgacagc ggcggtgcct gtctgctgac
     2041 ttatggaatc gccatgcagc gaagcactgc actggcacag gctgccggag taaccccttc
     2101 tgacctgcca gtccgggcac ttggtctggt cagcggtatg ttctatacga accgcctgga
     2161 tcagatcgga cttttcctcc ccagatatct ctacggccgg cattataaaa aaagtgcttt
     2221 tgccccctat gtatctccgg agcacccgga catcgtgacc agcctgccac ccagtctcct
     2281 gatcaccagt gacaacgata atcttcagag ctatactctg aattttgaaa aggctctgtg
     2341 cagacatcag atgccccata aactgatcaa ctacccgaag gatccccgcc tgacccatgc
     2401 gttcagcgct ttttacccag agcttccgga aagcagggaa gtgatccacc atttgattca
     2461 tttttttgaa cataccggag atgactgtaa aggaggatat gtatcatgac atacgacgaa
     2521 aatgttttca aagaaaaagc taatcggaaa gcccgtaaga tctggatcgt ttttgctgtt
     2581 ttactgagtg ccaactatgg ctccgatgcg tccgggggat tatatcccac cacttcttac
     2641 cttattttcc tggcattgtg ctggctgcct ctgatcttcg gcgaagtgct gttacgtgta
     2701 aagggctggg caacggagct ctaccgctac gatctggtca tcggttacgg catcttctat
     2761 acctttgtaa tctgtaccac agcgtcaccc atcgcattca cttacatcct tccggtgaca
     2821 agcttactgg tcctgtataa gaaccggaaa ttcatgatca actgcggtat cgtcaactct
     2881 ctgatcattg tgggcgctgc cgtctaccgc tacatgctgg gctttaacag tgcttccgat
     2941 atgaagaatt accagctgca gctgtcctgt atcatcctgt gttacatctg ctatgtcatg
     3001 tccatccgcc atctgaacga gtccgacggt gccatgaccg acagtatcaa agccgacctt
     3061 cacagagtgg tgaccaccgt agaacaggtc aagacatcca gcaataccat tctggacggc
     3121 attaccgttg tcagagaact ggcttctgag aacaagcacg gttccgatat ggtcatgctg
     3181 ggtatgaatg aattgacgga taataaccat atgctgcagg atcggacgac ctcttccaca
     3241 cagatgactt ccgacatccg tgcccaggtg gaaaatgttg tggcactcat cagcgaaatg
     3301 gtatctctgg tcgggaaaac cgaatcccat tccagtgtca gtgcaaaaga tctgcaaagt
     3361 ttggtaagca ctgccaccac gatgtcagag ctttctacgg agctggaaaa tgtcctgcag
     3421 aatttccagc aggaatttgg catggtgaag caggagaccg gtaccatcga aaaaattaca
     3481 aatcagacaa acctgctggc cttaaacgct tccatcgaag ccgccagagc cggtgaggca
     3541 ggcaagggct tcgcagtggt agctgatcag atccgtacct taagtactga aacccacgct
     3601 tcctccggac agatcagaga agctctgtcc cgtctggatg ctacttccgc taagatgacc
     3661 gcttccattg aagagacctt gaagctgatt caggtcactc tggaaaaggt aacacagacc
     3721 ggtgaaaatg taaatcagat cgcatctgat tccgcacagc tcggcagaca tattcaggta
     3781 gttgataacg caattaaaga agtcgaaagc tccaataccc agctggtatc caatatggaa
     3841 caggtatcca acatcgtgga gactatcacc ggctgtattg cacactccag ccagaccagt
     3901 gagcgtatgc tcagtaaata cgaagaaacc gcttccaata tcaacaccat cgaggatgtc
     3961 atggagaaca tgatgtgtga tctgggtatc ggcggcttca tgggtatcga agatattcag
     4021 ccaggtatga agattgatct gaaagtagcc ggccagggag atacggaata ccttggagaa
     4081 ctgatcgaac agattccgga gggtctgctg gtatcctgtc agaaggagct agcactcaca
     4141 gataccgctt cctgtacctt acagatcacc gcaggcaata tcctgtattg ttgggacaag
     4201 gcagtcatct ctcccgctcc tgaaaagggt gctcatgcct tcaaaatcat cattaatacc
     4261 agacccagga tcaataaccg ccgtaaatac ccccgtatgg atctggacaa tgcctgcacc
     4321 attaagttca aaaattccga cacggaatat gcagccacca tggataatat cagtgccaac
     4381 ggctttgctt tcctggcgac agacaatatt ttcacccaga gtaagaatgc ctcggtcact
     4441 gtcactatcc atgatttcgc cctcccggat cacaatgtgc tggaaggacg tatcatccgc
     4501 tgctccgatg ataacggtct gttcatcgta ggctgccaga tgccggagga taacttctat
     4561 attctggaat atgtagagaa aaatctggaa agtaagaaat agtcaaatac aaaagggtgt
     4621 cgcacagcgg caccctttct tgtttggttt tatagtttca tctatggctt caggagccgg
     4681 gcgatcagac cctcgacctc ctggagctgt tcttttgtgc tctccttgtg gctttccttg
     4741 cggtacttcg aaggggacat tcccttcatg gcgtgaaagg ccttggtaaa taccagggca
     4801 tcgctgtatc cgcaggacaa tgcaatactc tcgataggat aattcgtgga gtccagaagc
     4861 tctctcgcct tggtaatacg gaaggtagta aggaactgct ggggagacat ccccagatag
     4921 ttctggaaca gcgtatataa atagctgcgg ttgatacaca catagtccgc cacatccgtg
     4981 accttgatgg ggttacagta gttgctctgg ataaaggaga ccgctttttt cacatactgg
     5041 ttccccttat cctcttcctc ccttgccacc acaccggcgc tgtcggcgat caccgacaga
     5101 aatactccca gcagtccgtt ccggcgcaga tcatccgcaa tgccaaaggt attatgctcc
     5161 atcatatccc ggacaatgga atacagttcc tccgatttat cacaggaaaa aataggatgc
     5221 cggtccgaca gtcccatgct gtgtaagtat tcttcggcgc ggcttccagc aaagcccacc
     5281 cacagatagc tccagggatc cttcgcatcc gcctgataaa aagccagttc attgggctgg
     5341 atcaggaaac cgtaacccgc ctccagctgg tattccttgt cgtgaaaccg gaaatgtccc
     5401 ttgccgctca ggacatagtg gatcaggtaa tgtggcttgg aggccggccc gaagctgtgc
     5461 agcggtgccg tctgggagca tccgcagcag ttcacataca gacttcctgt tttatcattg
     5521 acaaaatcca gtttaaatgc tttttccatg atcggaccgt ttcctttccg cgttaccgct
     5581 ctctcatcta attcacgaga atcccaccgt tttcatcaaa ttccacgccg aagtcccggc
     5641 tgtattcatt catacgctgc aaaattctct tcttatcctc acctgctgcc ggagtaggct
     5701 taaaatcatt ccgggtggac acagaactca ccatgcaggg aatgatcctg gcattccggt
     5761 cctccacctt ttgtccgttt tcgaatgtaa aggtctgctg gaagatcatg gtgtctttat
     5821 ctgccgggtt ccggttggcc ccgaaacaga aattcgccag gctatagata atatatctgc
     5881 ccttatattc ttcgattccc tggaggacat ggggatgat
//
LOCUS       DAWDHD010000159         5917 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_778_length_5917_cov_13.201296, whole genome shotgun sequence.
ACCESSION   DAWDHD010000159 DAWDHD010000000
VERSION     DAWDHD010000159.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5917)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5917)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5917
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_778_length_5917_cov_13.201296"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1538 a         1531 c         1171 g         1677 t
ORIGIN      
        1 ttttttttaa ggatttgtgg accacagaag tcttcactta tgcgttcggt gtcagcgtca
       61 gatttgtatc atgatgatcc gtaacgatca cccgcatgcc cagagaattt gccattgcga
      121 tctggggtgc cgctgcaatt ccgttatcgc aggtcacgat cataccgaca ccttctcttt
      181 tggcctcttc gatcagatgc tcgttcaatc catagccgtc atggattctg tggggaatag
      241 ccgtgtccac ctgagccccc agcatctgaa aacctttggt cagaatatag gaagaacaga
      301 ttccatccac atcataatcc ccgatgaccc ggatatgcag cccttccctt accgctttta
      361 gaatttcctc cactgcctta tccatatcct ttaacagcca gggagaataa caatcttcca
      421 gtttcccgta gaggaacttc tgtatctcac tctcttccgt cagatcccgg tttcttagga
      481 ttctcgccgt caccggactg atatgaaatt ccttcgccca tttatcaaaa tctgcctttt
      541 ttgcggctac ataccatttt tccatacttt tcccactttc ataattttgc tcacgtattc
      601 gtgctttcgc actctacatt ctgcttcaca gccgattatt tttccctgag actcattaca
      661 ttgtaccata aaaattcttg tgcatcttct ctttttatgc tatagtcaat tcaatttcta
      721 ttttaaattt cttacatccg gcgtttcgcc atttttcaag caacttttaa tgacactaag
      781 gaggattctg cctatgaaac acaaacaagt attttccatg aaccttaccg gtcctattga
      841 ctacggtttc cgcaatgact atatgttccg cgctgccctg cagctaagtc aaaaggctct
      901 gatcggtctc atcagttctc tgctccatct gccaccatcc gcaattaagt ccgtcaccat
      961 taccaatcca atcactttag gtgcaactat tgaagacaaa gattttgttc tcgataccaa
     1021 tgtcttgttg aacaataata ctctcataaa tttggaaatg caggtgaata acctatacaa
     1081 ctggccggaa cgttccctga gttatctgtg ccgtaacttt gatcaactaa ataaaggaca
     1141 aaattattct gaattaaaac ctgtaattca cattggtttt ttagactaca ccctgtttcc
     1201 ggaacaccct gagttctatg ctacatatcg attactgaat cttaaggatc attttgaata
     1261 tagtgacaaa ctcacacttt ctgtggtaga cttaaagcat atagaattgg ctactgacgc
     1321 tgacaaagca tatggcattg ataaatgggc tgctttattc cggtctattt catgggagga
     1381 aattcttatg actgctaaaa atgatgaata cctgatggaa gcgacaaaga ctttatataa
     1441 tctgaatgcg gatgacatgg tcagacaacg ctgccaggca cgaatggatg ctgaactcca
     1501 ggaacagtat ctgctgaaga agattgatac tctgactgcc gagaatgccg cgaaagacgc
     1561 agagattgaa tctctgaaaa aggaacttgc cagtttgcag cagaatgtat aaccataact
     1621 taactttaaa aggagccgga acatttcatt ccgactcctt ttttgattcc attctactat
     1681 gtgaaatctt attttgcctt cggcgcaaat ttctttctca gtacatacca cagagcacct
     1741 gccacgcata cggaggaata agcaccgcag atgataccta ccatcaaagg caatgcaaaa
     1801 tcacggatac tggtcacacc ccagatgtac agagcaccta ccattaccaa ggtggtaagg
     1861 gaggtaaata agcttctgga taatgtctgc gtaatactgc ggtttacaac ctcctgcagg
     1921 tcatccttct tcgtcatgac tgccatattc tcacgtacac ggtcgaatac tacgatggtg
     1981 gcgttgatgg agtaacctac gatggtcagc agacacgcga taaaagtatt gctgacggat
     2041 actctcgcca gtgcgtagaa ggtgatcact accagcacgt catggagcag gcaggcaata
     2101 ctgctgacac cgaaacggat atcgctgaaa cggaagcgga tatagatcag catgcagatg
     2161 attgctactg ctaccgccag gatcgtatcg gacttcattt cattactgat ggtggagctg
     2221 atggtctcgg aagtaatcag agactcatct acgccgaagt tatccacaaa tacctgattc
     2281 agagcctgtc tctgatccac atccagagag ttggtcttga agataacttc attggaaccc
     2341 tgcacggtct ggatctgtac tgcactgccg gtggtgctct cgatcagagg aaccatctgg
     2401 ctattgatct catccagggt atagctttcg ttgaatgtaa cagtagttgc ggtaccgccc
     2461 ttgaactcca gactgtagtt cagtgcatca ccactgttta ctttatatac tcccatggta
     2521 atgaatccaa ccaggatcac tgccagagac agtccaaaga aaataccctt cttgccgagg
     2581 aagttgatcg gcttgtgctc cttgccgata ccataccact tagtatcttt cagtcccagt
     2641 gcgtagaaag ctttcataat gtacttggtc actaccagtg cagtgaacat ggataatacg
     2701 ataccaagtg ccagcgtctg tgcgaagcct ttgacagtac cggggcctaa cagccacagc
     2761 actgccgctg cgatcagggt agtcacgtta ccgtcgataa tagcggacag tgccttgtca
     2821 taaccgatct gcatagcact cttaacagtc ttgcctgttg ccagttcctc acggatacgg
     2881 gcaaagacga taacgttggc atccactgcc ataccgatag acaggatgat acctgcgata
     2941 ccggacaggg tcagggtcag gtcaaagccg ttcagcagca atactaccaa tgccacataa
     3001 atacccagtg caatgacaga agcaaatccg ggcaggaaat atactgcaat catgaaaaca
     3061 gccactacga taaatccgat cacagctgct tttaaactgg tggagattgc ttccacaccc
     3121 aactgtgcgc caactacctt ggaatgcagc tcttccagct ccagcttcag tccaccgata
     3181 cggatggtgg aagccaggct ctctgcctct tcataggagt tcataggaga tatctgtgca
     3241 ttaccatcgg agaacacacc ttttacggaa ggaacactta caaaggatcc atcatagtag
     3301 atacccaggg tctcgccctt ctcgtaagca tttctggtag cctcttcaaa cttcttagtg
     3361 ccttcctccg tcatcaccag atctaccgca taagtggaat tgcccatcgt ctggtccttg
     3421 atagagcctg cggtagcagt cttgacatct gtaccctcca gcatgatgga tccatctgcc
     3481 ttcagttcat cgatggtctt attcagagcg tacgcatagc ttacattgcc ctgtgcatcg
     3541 gttactgcct gcatagagta gttcgcattg ccgtccttgt ctgtctctgc aatgaaatac
     3601 agggaaccgg gacgtcccag ctcttccagg atcttgttgg catctgttac accgggaatc
     3661 tcgatattga tacgatcggt accctcctgg tatacaatag cctctttact gtagttctca
     3721 acacgcttct gcagcttggc aatagtatct gccatatccg ttgcatccgg ctcttcatcg
     3781 cctaccacct gataagtgat actgacacca ccggccagat ccagtcccag acttatgtct
     3841 gaagcactgg cagttccttc cgcatctacg ccatacagat ccacatagcc gacacccagc
     3901 aggaccagaa gggcgcagag cagaatgatg attgctttac tttttttcat ctttcctttg
     3961 tcctttctat tcttctgaag cttctgctgc ttccgcagcc ttcagcatga tagcacattc
     4021 gatacgcttt ctctgcagct cacagccgat gtcatacgca tcattgatat tgccatggaa
     4081 attataggag ttcgcagcac atccgccgct acaatagaac ttggcaaaac acttcttaca
     4141 cttgtccttt gcatagacat tacagcattt gaactcgtca cggatatcat ctcttaccac
     4201 accggtatcc acattgccca tgaggaactt ctcgttaccc acgaactgat ggcagggata
     4261 caagtctccc cagggagtaa ccgccagata ttcggtaccg gatccgcagc cggagagtct
     4321 cttggctacg cagggaccgc cctgcagatc gatcataaag tggaagaaat tgaaagcctt
     4381 gccctctttc ttacgcttga tcatttctac tgccagttta tcgtattctt cctttaagat
     4441 cggaagatcc tcttctctga tggcataatc atccgtaggc tgtgccacaa cgggctccac
     4501 ggagatctgt ttgaatccca gatctgccag atgcagaacg tccttggaga aatcgagatt
     4561 attgtgggtg aacgtaccac gcacataata gttcatctgg tctctgctct ccgctacctt
     4621 ctggaacttg ggaacaatga tgtcatagct gccctgtccg ccacggaaag gacgcatctt
     4681 gtcgttgatc tccttacgtc catcgatact cagcacgata ttacccattt ccttattcac
     4741 gaactccagg atctcatcat ttaataagat accattggta gtcagggtaa aacggaactt
     4801 tttattgttg gacttctcta agcttctgcc gtattctacc agctgcttta ccacatccca
     4861 gttcatcagt ggttcgccgc cgaagaaatc cacttccagg ttcacacggt tgccggaatt
     4921 ggctaccagg aaatccagtg cctttttgcc gacctcaaag ctcatcagtg ctcttctgcc
     4981 gtggtactca ccctcttccg caaagcaata tctgcaggca aggttacagt catgggcaat
     5041 gtgcaggcac agtgccttga ctacggtctg acgcttctta aaatcaaaaa tgtaattctc
     5101 ataaatatcg ggagcaaaca gctgtcctgc cttctccagt tccaggacct cgtccaccgc
     5161 ttcggtgatc tcttcctggg gataggggat atttgcaaga ttcagtacag cagccttgat
     5221 ggtgtccgca tccttgatac cttctgtcac cagaggctct accagcggaa tcatatcata
     5281 cacaatatca tctaccacat ggacagaacc actgttcaca tccataacga tattatagcc
     5341 gttgctctta tactggtgta tcacagttct attcctcttc tttctacttc ttattgctta
     5401 aaatacatta acattaacta ttcggaaccc cattcgcaaa atcatcctcc ggactttcct
     5461 gaataacatg cctgcatgtt atttgctcat gtggttactt cgccgtattt tgccggacgt
     5521 atcttcttac gtatgcaagc atccgacaaa gaatacgctg tcatctccag ctccgaatag
     5581 ttaacagaga ataagcagcg acctgaatcg ctgcttacgt tagtctcttc tcattttctt
     5641 agattattta atcttttcac aactctggtt tcctacagtg caggaagtct tacatgcaga
     5701 ctggcaggaa gtctggcact cgccgcagcc gccctttttc atggattcct ttaaatctct
     5761 ggttgttaaa gtcttaatat gcttcattaa aaatagcctc cttatgattt caaagcgcct
     5821 atagacgcca gcgtgtatat aaacatacag acacgcaact catttttgct cattatatca
     5881 tgtatttggc atttgtaaaa gctttttttc tcaaaaa
//
LOCUS       DAWDHD010000160         5821 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_793_length_5821_cov_11.048214, whole genome shotgun sequence.
ACCESSION   DAWDHD010000160 DAWDHD010000000
VERSION     DAWDHD010000160.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5821)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5821)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5821
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_793_length_5821_cov_11.048214"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1465 a         1564 c         1035 g         1757 t
ORIGIN      
        1 cccttttaaa aattccaaac tgaaatcttc cgataaatcc accggttcag tctcgtcatt
       61 tgatacagca gtagtagtta tggtattagg tgtcatctcc tcggtaactt ctgaagtatt
      121 ctgttctgta tcattctgtt ccggtctatt ctgcccacag cttaccatgc ttaacataat
      181 tattaataat gccacacacc tcatctttgc tttgtacata ctttttctga atcctcctcc
      241 cacaagattt ttcaaaagcc ttttaccata cgtattcctc cagcatcctc tttatagaaa
      301 taccaattct ttcatcctgt ttctcactct ctgtaagcga caatgtcaca ctcaatggat
      361 cttccagttt cttattctta aaatcaataa agtatcccag ttccagaaca acgcatccta
      421 tttcttcccc ggaatgtacc tgcttcattt ttctaattcc agattctgtt atttcttttt
      481 tggtaattgc ataagtcgga tattcattat ccgaaagtaa tgagtactca catagtgcag
      541 tgattcctgc ctttttttcc aattgaatat cctcttttag ctcatacctt gcaatcacag
      601 ggtttctcaa tatgacccgt acatcattcc acagtttctg aatatccgcc ggaacagtta
      661 tcaccctgga tttacccttc atacccaaaa tatccacatt cagatactca atttcatcaa
      721 agcacctgtt tgcagacatt ttcgtaacgc ctaatcttat tgcagctttt gatgcagtaa
      781 cattttccca tttttcatat attgcaacca atataacttt ttgtgttaaa aatgaaatta
      841 aatgaaccgg agaaattttc ctttcattct cattggacag caaatatccg ataaaaggca
      901 agtacacctg tttatctttc agtacaaagg gaattccctc ttccaataat ttttctttaa
      961 tataaaatga tgtagaatca aaaaaaatgg cgcaattaag acctgctgcc ttttcaattt
     1021 taatccagtc ctttctaagc gctgccaggc caacatctgc cttgggttga attgctatcc
     1081 atggtgaacc gtttgtttca accttataaa aagtgtatct tccacgaaag gccaggggca
     1141 gcttctcatt caaatattca gtttcttcta tcatcacatt ttgacgcaat gttttttcca
     1201 aaaaattttt catatagtca ccatttttaa aaatgtaact ctttttatgt tacattttta
     1261 aaatagcata tttttaatac acattcaata tttaatgata agatagtcga ccgatatcaa
     1321 ttattttgat attttcaatt taaaatatcc caatcacata atcataacta taggtgtgca
     1381 catacaaccg gtcatccttc tctgccatga ggtccggcat ctttttcagg ggcaggcggt
     1441 cgatcacctc tccggtattt ttatccagct gatagagata atcgtcctct gcggtaaagc
     1501 catagccgca gaagatcacg tcccccttca cgagaaaatt catgctgtta taagtctggt
     1561 ctgcacttcg ccacagcagt ttctcatttt ccagatcata ggcaaacata aagcagctgt
     1621 ccggctgcgc ataaccgttc attactgaag ccccgtaaaa aattccgttc tcaaggcagg
     1681 cattattacc gttgacgtac catttgtctg tcggatattc cagtacatac aggcagttct
     1741 caccatcgaa aatgctgagt tttccatcct cccactgata ggtatactcc cactgatagg
     1801 tataattttc atcatagaag cagtcccagt tttctccgat catgggaagg gaaatgtctt
     1861 ccgatgcatt gtaccattcg tctgcaaaag taatattgtt atacgtctcg gaaaggattt
     1921 ccagggaata gggctgcgcc gcagttcctt ccgggtgaaa ttccgagatt ccagtgaaat
     1981 ctatgaggat ctctttcggt gcaatgggtg tttcccggga ttcggactct gtgagagctc
     2041 cggaggtctc tgcctcatct attttagtgt ctatattgga aatattttcg gtttgtttct
     2101 ccgcttcgtt gttctccggt gcattggtct cccgggcgcc acatgccatt aacaggcagg
     2161 cggacagtgc aaatgttgct aatctgtatt gttttttcat gataatctcc cttactgctt
     2221 aaaattctcg taactattca gaaccccatt cgcgaaaccg ctacttcgta gcttcttcat
     2281 tgatcatctg ttcctcttct ataacagtaa caataccttc atatacggta aagtaatatc
     2341 gaatctgctc ctgcatgtcc tcatactgcc aacgttccca ggaatcttcc tcaacagact
     2401 tcacctgcgt caatgtgtca tcggcatccg cgatgtttcg ctgtaattcc tccagcgttg
     2461 tcttatcgac aaggaatctc ccatcccggc tgctgccggc ctgaatctgt atcctgtcta
     2521 atccaccatt ttcatcaaaa taacaatagt atttgctgtt ctctatatag agacttgcat
     2581 tctcactggc gacatcgcat tgcaatccgg catcggtaca caatgcccgt acttcttccc
     2641 tttgcattcc ccattgcatc cttgacagga cctctcgatt atactccagg gaatagacca
     2701 ctgccttcac cggcacaaag gagaaggagc agcggtgcga tcctgcctca tccagatccc
     2761 cctccatcac tgtggtcaca gagtccttgc caagtttgtc tcccgctccc aacaacatac
     2821 catcttccaa taaggcaaaa ttattatatc catatcgaat agcagagata tctgcatcaa
     2881 aggtgcgggc atcatcgctg tgccggattc cacccaacca taccatactg accttatcca
     2941 gtaccttttt gggttctctg acaaagtcat gaacattctc actcttagaa acgccgcact
     3001 gtccaaagat atttaatccc caaagatata attctccgtc ttccgtgatt gccgctccat
     3061 tccacgcatt gatgtccaca tagatacaat gatccagaac tttatgtggt tccagataca
     3121 tcatttttac cagattcaac ggattctctg ttactgacca gtattcatca tatcccccaa
     3181 cggtcgtgct gcttgtcgtc cgaagctgtc cccaccaata tacggctcca tcttccttga
     3241 gtacgataat ggcttccctg ccggctctgg catatctgac atttttcatt aacagtgtgg
     3301 gggtacatac cctgtcgtta tccatgtcgc tatatacctg cttcacagtg tcttttccca
     3361 acaaaagtcc tgccatattt aatcccagtc cgtagaggtt tccatcctcc gtcagataaa
     3421 tgcagaaatt attattccaa tcggcatcta cggaaaccac atgatctgca atcctcacat
     3481 catcaaactg ccccttgtca aaatccgtct cgtaactgcc tgttcccaac tgaccattct
     3541 cattataacc gttcccccag agaataccat cattatcaat ataataatga ttataggcag
     3601 tgacccgctc agtgatataa tagttttcaa tacgaaaagg actgggagct tgcagttcct
     3661 cttcctgctg tatgtaaatt gttggcagat tctgttcctg tacttgtcct gtttcttccg
     3721 ttgtcaccat ttctgtgaag tcttcctctg ttttctgaga agaaaccgca cttttctgtg
     3781 tgagcagtac cagtgctgca atgatacaca gttccactgc cagcgccgca agcttccctg
     3841 cggtcttacg gcattttaaa atatgccgga tcctgccccg gatgtcacct tcatcaaaac
     3901 acaccggtgc tgcaaatatc ctgcgtgcgg tcgtatcggt tgccatggat agcagtacgg
     3961 cggcatattc ctgtcggtcc tccggctcca gatgttccgt cactgcttca tcacaggctt
     4021 tttctatatc acagcacagc agataatatg ccaaccagac caccggattc atccaatgca
     4081 caatactgat cagataggtc agtattttcc acagatagtc ccggcggtgc agatggatcc
     4141 gctcatgctg caatacacag tcataaaatt ccggcttcat ctccatcggc agatagatct
     4201 gcggccgcag aatcccgaat accatcggtg tgcggatgcc gtccacgtaa cggactccct
     4261 cctccccggg aaggcattct gccagacggt gcttcattct tacataagaa aagataaaat
     4321 acgcgagaaa acaagggatt cctgccagcc acaggtatct gactgcaaat atcacctgtg
     4381 taaaggcggc cgataggaaa ggcatgactg ctgtgccgtt ggactgtgca gccgtcacag
     4441 ggagctcgtt cgatcccgct tcgaaattcg taccctgcac ggtcgtcgca tctgttccgt
     4501 tcacctgttc cggcaaggta ctctgtccag gtgtaacatt ttgctccggc acagcacttt
     4561 gccccaacac aatgcccgtc ccaagtcccg tcaccactgt cggtccatcc gcgctcaccg
     4621 ccactaccat cgactccgac tgtgcccgcc agaatccgtg ctcattctcg atcttccagg
     4681 gaaatataag atagaaaaaa acgattcccc acaatagcac acagtagcgg taggaaatcc
     4741 gcagtcctct aaataccatt ctgaggatcc atacgattcc tacgatgatg cttccctgaa
     4801 tgctcattct gatcagtatc tgcatcattg ccatccctgt cattcctttt ctcccccgtt
     4861 attatctatg atcttcttca gttctttgat ctctttcgta gacagctttt tccgggaggt
     4921 aaacgccgcc acaaaagcag gcagggatcc cgcaaaggac tcctcgatga tcacctcact
     4981 ctgtctcgcc tgaaacatct gcctcgacat cagtgaggtg acagtcccgt tctcactggt
     5041 aaaaatcccc ttctgacaga ggcgccgcag gaccgtatag gtcgtagatt tcttccagtt
     5101 caattccttt tcacataatt tcaccagttc ccccgaagcg accggctcat gctcccagat
     5161 gatttccgca aaacgcatct ccacagctcc catggtatat tccgacatta caattcctca
     5221 ctttctgccc tctgtggcat ccgttaagtc ttattataca aaatggttta caagttgtaa
     5281 cattttaatg ttacatcctg taaaccattt ttgtcaatgt ctattttttc cctacaaaag
     5341 taaaagtttc tcttataccg gaaacttttt tcattttttt caagtgtatc cagtataaat
     5401 tgttctcatc ccccattcgg tatcagatca ttgaaagtgg cccacgcttc ttccgccgtg
     5461 taatccgcca gcttaagcca cccggtgaca ccattctcgg tctccacgca gagccactgt
     5521 ctgccggcat ccagataaag gaaatagact ccgtgatctg cgttgatggt aaagcgttcg
     5581 ctgccggcat ccggagtccg gcagacttcc agatccctat tgagccagtg cagggtcgac
     5641 tgctccggca gatcatagcg gtctgcagga atctccgcca gtcttccgtc acttccaatg
     5701 cggtatacga tccgcaggtt ttccttctgt aacgtataat catagggctg gttggtgatg
     5761 atctggccgt tttccaccca gatctcccgg atatcctgcg cgaagcttcc tacctcctgt
     5821 a
//
LOCUS       DAWDHD010000161         5804 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_800_length_5804_cov_8.971995, whole genome shotgun sequence.
ACCESSION   DAWDHD010000161 DAWDHD010000000
VERSION     DAWDHD010000161.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5804)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5804)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5804
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_800_length_5804_cov_8.971995"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1427 a         1576 c         1218 g         1583 t
ORIGIN      
        1 tcatataaga atacgatctt agggaacaat acaggcttct tgtggtcctt tttgccctgt
       61 ccctttctgc ggacatccag catggtaata gtcgccatct ttgcaaattt accggtgccg
      121 gttcctgcgg tcacggtaat aaacggataa tcaccacggc tgctggccac agtattgaac
      181 ttgtactccc acccctggaa accctgttca aattctttct ggacatctgc cagtgcctct
      241 gcttccgcca catctgcggc aatacccaga cgctcatatt ttgccagcgc tctgttataa
      301 gacttctccg catacggctc caggatcaga tccacgctgg gtacggtgaa tccgccgtac
      361 tgctggctgg ctgcacttaa aacgatatcg ccgataacat caaacgccac atccagtgtc
      421 ttcggctcat tgtaccagat attgcccatc tcaaatccgc cggtcagtac actctgcacg
      481 tcgaacagac aacaattcat ggtatctctt ctggcggaca tgtcatggac ataaatataa
      541 ccgtcccggc aggcctggat ctcttccgtg gtcaggaaga acttctggta caattccttg
      601 ttcagctgat tgaagatcag gctgcgcttg gtggatacca gtgcggaatc cgtgttggca
      661 ttttccttgt ctccgatgta catgatggac tggctctttt tgtagacatc atccagcatc
      721 cgtacaaaat cctgcttata gttacgataa tcgcggtagc tttttgcaac gaccggtttt
      781 acctgctcta aggcactttc cacaatattg tgcataatgg gaatgggtac ttcatccact
      841 tccagttcat ttactttttc taccacatgc cggcagatct gctctttttc ttctctggtg
      901 aattttgtca gggcacggta cgcactcttg cctacagcgc tgatcacctt ctgtacatta
      961 aaaagctctt tgctcccgtc ctttttaacg acgtagatct ctctgtccgg tctgaacttt
     1021 ttgccataat cttcattttc ttcatgggca tgttcaaaat tactgctcat ctttctcttc
     1081 ccttcatccg ataatccccg gctgttgccc ggatttactg tattcccctc acttacgata
     1141 acgtttgtag ataccatata tggtatctgt agtcatcaaa aagcaatata ttgtgggtga
     1201 tatgtaccag tataaaaaag aatccctggc ttgtcaacga gggatccctt agaaaactat
     1261 acaaaaaaga ttttcaaatt ttgggtaact ttttattccg cctcctgtga ctgttcccgt
     1321 aaaatatctg aaagccactg tacatagcgc ttttgtaccg cttccggcgc tacgatacgg
     1381 gccccctgtc cgataccggc cagccatccg aagaactgtc cgctgaccgc cacctttgcc
     1441 cggatccgga acacttcctc cgacatggga cggatatccg tctcccttcc gaagcggtcc
     1501 agtaccacgc cggtcaggcg caccggaaac tccatggtca ccacgctctc ctctccggca
     1561 aacatgccga aggtctggtt cgtataggag gtcacatcga tctgtgaaaa ctgttccact
     1621 ccttctctgg gcagatccgt cacttccgcc tgtcccatct tgtccacacg gtaatgcttg
     1681 atcactctgt cttcactgtc gtaggcggcc agataataat tttcctcccg ccagatcagg
     1741 gcccagggac tgacgcattt gttgccgttt ggtctgggca ccagctgctt tttaagattc
     1801 cagtccaggt agacgaaaga gatctgctta ttttcctgaa tagcccggtg gatcgcatca
     1861 atgctgtaat aaatgctctc attctccgtc ttgactcttc cggccacata gacctgtcgc
     1921 tgcagtttac cggcatcgtt tttcccggcg atctgctcca gcttccggat cagttctctg
     1981 gattttttcg tggtgataaa ccgggaagac tgtaccgcgt ccaccagcag ctttaattcc
     2041 ggaagttcaa actcccggct gcccagataa tatccgcctc ccagtctgct ctgcacctgc
     2101 accacatcat agccgaaatc cgcaagacat tccatatcgt tgtagatact cttgcgctct
     2161 gcatggattc cctgattttc cagatatttg atgatatccg gtgtgctcat tggatggttt
     2221 tcatccgtgt tctcccgcag catcttgatc agatataaaa gttttaattt ctgtcccgaa
     2281 gattttgcca tggattattc ttctgtctgt ttcttttcct ctgctgcatt ctttttcttg
     2341 cgcagagtga ttcccaacgt aatggccact gcacctacaa cggcaatgac taccagtact
     2401 accagataag ataaaaatga attcaaaaag cttactgcca taatgtcttc tccttttccc
     2461 gatatctttg tatcccgaaa ttattgcatg tattagaagt ataccaacga aatccccgta
     2521 tcgtcaagtg gtcccttcgg atatggccgc cggtgtattt atccattttc ctgtcttaag
     2581 aaaattttcc gcctgtccgg aagccttctt tatgatctgt tcctcatacc ccatcatctg
     2641 aagcagacgg atcgcatttc tggtggatgc ttttcccggc agcagctgat aggggaacag
     2701 gatatcaccg acccggatca cttcctcaaa atgatagttg tcatagtatt ccttcaaaag
     2761 ctccgtcatt tcaatatcat gggtagcggc aaaaccaagc gttccgctct ccgccagacg
     2821 gatcaggatc tgcgttgccg cagcgatccg ctccaccgta ttggttcccc gaagcacctc
     2881 atccacaaaa caaagcactg gagttctccg ttccccggcc gcatccagga ttcgtttcaa
     2941 tgaccggatc tccacaatat aatagctctc cccgttttcc atgctgtccc gcagggccat
     3001 ggaggaaaag atctgaaata tcggtgcatg ataactttcc gccgcgcagg tatggatcgt
     3061 ctgtgccaga gcggcattca ccgccaccat ttttaagaac gtggatttac cggaggcatt
     3121 ggaccccgta agcagaattc ctctgtccgc ccggatactg tttttcaccg gctgtgacaa
     3181 cagcggatgc cagcccttct tcatatgcag aaaagctttc tccgtatctt cttccagttt
     3241 cggaatacag tacccttcca gagatttccg aaatccgcct acggatatgg ccatatccag
     3301 gtacccggtg attgcaagca tggtttccac ttgtccggtc tttttccgca gcttttccag
     3361 cttccggtta aactgaaaga tatccagatg caatatcatc cgcagataat ccgcaaggat
     3421 atccagagga tttccaccaa tctttccctg atttccagcc atgacccaat aggctccccg
     3481 cctcaactcc cgcaggctct tcagtgcctc ccgaagttca tccagttctc tcctgtatac
     3541 cggtattttc tgcttctcca gttcttcaca tacatcgatc agccgtaata cataagcgaa
     3601 gctgataata tacggttcga tcactttttt ctcccgaaaa tatgtcatga tatgccagag
     3661 catacacaag acgattccta tggtacccat cgcaggctgt acgaacaata acaggagaaa
     3721 tggaaggtac agcagatttc ccagcacaat cttacggttg gaacgctccc ccagataatc
     3781 caggttatcc agataatcgt agagagaata ttttcccaga tgtcccagcc gtttcatcaa
     3841 cagctggatc cttactttta tattctcctg ctcctgcagc cagttaacca cttcttccag
     3901 atgttccagt gcctctctcc cgcaggaaat attccggagc gtatagtaca gatattcctc
     3961 tcccgccgca gaataggtcc ggtcgatccg gcgaaagacc tcgtccattc ccagatcgtt
     4021 ccatgtgata tcatccagct gcttctcttc cttatgccgt tccagatagc tgcccagtct
     4081 tgcaaacctc tccagtgaat attccttcgg tggttctttt ccgtaatttt cacgaagata
     4141 cttcttgaaa agtttttcct gatttttcgt ctgtatcaga tcctgcacta tcataaatat
     4201 aaaaagcaaa agcaatacgc ccgcaaatac aacaaattcc atattttttc tctcttttcc
     4261 ggatataaga aggcaggcac cgccctgtgc agtgcccgcc cccatattct gacctgtttt
     4321 ttatatttct tatttgtaaa actctttatg cgacaataaa gaactttaac aggaagatca
     4381 aggataatac ataggtaagt acggatacgt ccttagcttt accggtaaat accttgataa
     4441 cggtataggt gatcagaccg ataccgatgg catgtccgat agatccggag atcggcatgg
     4501 cgatcatcat cagagccacg ggaagcgcct ggcagatgtc atcaaaatct acattcttaa
     4561 gacctgctac cataaggaca cccacataga tcaatgcgga agaagtagct gccgcaggaa
     4621 tgataccggc gatgggtgcg atgaagatac atgcaaggaa cataataccg gtggtcagtg
     4681 cggtcagacc ggtgcgtccg cctgcttcca caccggaggc agactctacg aaggtggtaa
     4741 cggtggaagt accggttacg gaacctgcca gagtgcctac tgcatcagac aaaagtgcct
     4801 gcttcatgtt aggcattttt ccatccttat ccagcattcc cgcacggctt gcggttccta
     4861 ccagagtacc gatagtatca aacatatcaa tgatacagaa tgtaacgatc agagtaattg
     4921 cagtaaacca gccaatctgt gcaaagccct ggaagttaaa cttaaacagt gtagtggacg
     4981 ccatatctcc gaaggccggt acgaaggatg cagttgccag agaagcaaag ggattggttc
     5041 ccaggaaaat ggcctctcct gcccagtaga tgatgctggc gatcagcatc cccagtaaaa
     5101 cagcaccctt gacacctctc tgtgccagaa caacaatgga aaacagacct acgaacatgg
     5161 taacaaccga cagcaccatt gcggaatatc cggatcccat attggtctta gccagtgaag
     5221 gagtcagtgc accgaagaag tctctcatag catagaaagg tcctccgttc tcggaataga
     5281 ttcctgcatt ggaaccgacg ccgatgttta acagcataag accgatggcc ggtgcgatac
     5341 cgagacgtac gcccaaagga atggcatcca cgatcttctc acggatattc agcacggaga
     5401 ggatcaggaa tacaatacct tccaccagaa caatacacag ggcagcctgg aaggatgcca
     5461 cataggtcat gccggtcagg gctacgatat tggttactac gactgcaaaa aagctgttca
     5521 gtcccatgcc gggtgccatg gcaaaaggct tgtttgccag aaatgccatt acgatggtac
     5581 cgatggcgga tgcaatgcag gttgccagga aaacaccgtt ccacagctcc ggcatggtat
     5641 ccttgccaaa accggtcagc aggttcggat tcagggctat gatataagcc atggtcatga
     5701 acgtggtcag tccggcaatg atctctgtcc ttgcggtggt gttgttttcc ttcagtttga
     5761 agattttttc taacatcttt tccctcctaa gtattctggg agcc
//
LOCUS       DAWDHD010000162         5764 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_809_length_5764_cov_9.641443, whole genome shotgun sequence.
ACCESSION   DAWDHD010000162 DAWDHD010000000
VERSION     DAWDHD010000162.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5764)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5764)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5764
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_809_length_5764_cov_9.641443"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1354 a         1551 c         1274 g         1585 t
ORIGIN      
        1 atgtacaccc tgctcgtcat gggtaatgcg gaaggacttg tgcaggccga atttgccgac
       61 accgtattcc ttatagaact gggtcagtgt agccttcatc tcctcggccg ttccgtctgc
      121 gcagaacttt tccgccagtt cacagatgcg ggcacaaatt ctcttattgt acaccttgct
      181 ctcctgcatg ctgctggcat aattttcaat gatggaaaat tccggcacat gaagctgctc
      241 catcatgggg gcaaaatcaa agtcatagaa ttccttgaaa atgcggatgt catgcagtgc
      301 cgcatcgttg atggaaccga cgatctctcc gcggatctcg cagccacagc tgtagctgtt
      361 ctcgttattc accaggagat tggccagata gcagtgccac agattcccgt ggaaaccgta
      421 attacctgcc agatcgatca aatcatgaat gcactcgtaa aagagtgccg ccatatcttc
      481 cgtgttgtaa tattcgtcat cgtaatggga catgaggaac gccatgtcat acagcagttc
      541 tccgtcctcg aaatcacggt atacgatcag ttctttttct ctcatatgtc caaccttctt
      601 gttttatatg ttttaactat tcagagccaa tgggcgaagt aaccacatga gcaaataaca
      661 tgcaggcatg ttatttaagt aagcctcgca gaggcggttt tgcgaatggg tttctgaata
      721 gttgttatgc ttaataattg cccagtatct tcatgctgcg ggcttcttct ctgagtcccc
      781 gcagtgcgtt tttcaccgcg ctgtccgcaa gattgccttc gaaatcaata aagaagcggt
      841 actcccagtt cctctcgggg atcgggcggc tctcgatctt ggtcatgttc agattgttgt
      901 agataaaatg ggacagaatg tggtacaggg atccgctctc atgggggatt tccagacaca
      961 ggctgatctt gttggcattt ttgcggaaga tcttctggtt cgtcacaatg atgaaacggg
     1021 tggagttgct gtcggaatcg ttgatccctt tttccagaat ctccagtccg taggtctggc
     1081 ccgcatattc gctggcgatg gccgcctggg tctgatcctt gtcctttgcc accttcagtg
     1141 ctgcaaaagc gttgttctgc atgctgatct gctgccagtc atgctcggag aggtatctgg
     1201 cgctctgcat cagggactgg ggatgggaat agactctttt gatctcttcc atcttcgtgc
     1261 cgggcagtgc cagcaggcag tgttcgatgg ggatcacctg ctctcccacg atgtagtttt
     1321 cgaattccac cagcagatcg tagatttcat tgacaatgcc cgccgtggaa ttttcaatgg
     1381 gaagtacggc aaaatccgca ctgccttcat caatggcact catggcatcc cggaaggatt
     1441 ccacatggaa tgcattgaca ttctcgccaa aaaatttccg catggctgcc tgggaatagg
     1501 aaccttccgc accctgatag accactctgg cacactcggt ctccagttct tccacgccga
     1561 taaaaggaag ccttccctca cttccgtggg cggccaacag ctggtactgg agctttctgc
     1621 tcatggacat gatctgttca aacagttcct ccacgccgtg gctgttgaaa tcattatggg
     1681 tcagtgcctt gactccggcg atcttctcct gctctctctg tttgtcaaat acttttttgc
     1741 cggtctctat tttatattcc gccacctgtc tgctgacatc catgcgcttc tcatagagat
     1801 ccacgatctg ccggtcaatg gtgtcgatct gttgtctcaa ctgtgataaa tccatgctgt
     1861 tctccttaac ttaactgata tccacgaatt gctccgctgc aaagcagctc ccgcaattct
     1921 caaacattcg gaatccgctg actccaaatg taactattca gaaccacatt cgcaaaaccg
     1981 tctctgcgag gcttttctga ataacatgct tgcatgttat ttgctcatag tggttacttc
     2041 gccctttggc tctgaatagt tacattctat ctcacttttt tcttgttagc aagcaagaac
     2101 cgaggtataa tattagatat tacaaaaact ttacatttca ttttacataa agaaggattg
     2161 cttatgaaag ataaaaaagc cttattgatt ctgattcctt ttcttctgtt cgtgatcatc
     2221 atcggtgcat ttgccatcgt gggagaccgg atccctgaca atcccccgga gaccgtgggg
     2281 aacaccgccg gaaatctgaa caattccggc tacttctgcg agtatgatgg aaaagtgtat
     2341 tttgccaatg tctatgacag cgacaccctg tactccatgg acccttccga acaggacatc
     2401 aaaaaactgg ggaatgcctc cgtacaaaat atcctggctg gcggtaaatt cctctactat
     2461 tatcagaccg gagcttccgg ggatgccggg atcggtgctc tgcgaactgt gcgttccttc
     2521 aaccgctgta agctaaacgg cagcgacgtc acaggactta ccagagatcc catttccacc
     2581 gcccagctgg tgggaagcaa cctctatatt atgaccgtgg atgataacgg gccattattt
     2641 taccgtatga agatcgataa atccgataag acagagctgg ccaacttcga gatcaatccc
     2701 gccagcgccg ttaacggcac catttactac aacggtacac aggacaacca ctatctctat
     2761 gccatgaata ccgcaacgga cggagtttcc accgtatggg acggcaatct gtggtatccc
     2821 atcgtacagg gagattatgt ctactatatg gatgtggaaa acaattacag actgtgtcga
     2881 tactccctgt cccgaaatga gattgaagtc ctgaccaacg aacggatcga ctgcttcaat
     2941 gtgggttacg gttatgtgta ctatcaggta aacggcgagg aagcctgcct gaaatgcatg
     3001 cgggatgacg gaagtgattc ctgggtcatt gccgaaggca attatactgc catcaatatg
     3061 acatcccagt atgtctattt ccagatgttc ggagatgact cttcctggta tcactctcca
     3121 ttaggcagtc agagctacag cggatttgac gcagccagac aggccgcgtc ggatgctttg
     3181 aaaaaataat gtaacgattc agagtcatga aatattagcg gatgttcgtc gaatgcttgc
     3241 atttgtaaga gcatccgctc atatttcttt ggcgaagcag cttgtatgag caaaaagaaa
     3301 gcatcgaaga tgcgattttg cgatactgct ctgaatagtt acagaataat aacatagcaa
     3361 gagccgcaga cattccgcct gcggctctta tttttcgttc ccgttgcaaa ttgattttaa
     3421 ccttcctcga tatggttcag gccctgattc agaatcgtga tgatatggtc atccgccagt
     3481 gcataaaaga tcgtcttgcc gtctctgcgg ttcttcacca gatccatctg cttcaatacc
     3541 cgtaactgat gggaaatggc agactgtgac ataccaagca gagtggctat gtcatacaca
     3601 cacatttcgg agcacagcag caccgacaag atcttcagtc ttgtagcatc tccaaatact
     3661 ttaaaaaatt ctgccagatc ctgtaactcc tcctccggcg gcatcttctc atcgatctgc
     3721 cggatggttt ccggatctgc ggaaagataa ctgttcttct cttccatttt atttctccat
     3781 tatttactct gtgatccctc tctcaacaga ggttttctgc actgactgtt atctacggtt
     3841 aactatattt actatacact atttctgcga attcgacaaa tgaaaatatt gccgcatgcc
     3901 ctctgtcatg gtgatcgttc cctcatggtc gatgaacaat ccttccgcat catacttttc
     3961 cagaagttgc tggccttcct cctgtcccag tacaaagcag gccgtagaca gggcatcact
     4021 taagaatccg tccttcgtca cgatggtgac ccccgccaca tcactgtggg ccggtgatcc
     4081 ggtccgtgga tccagtatgt gatgatatct gacaccatcc acttccacat agcgttcata
     4141 atctcccgat gtggatacac agtgccctcc gtccagtgtc agcacgccca cactttccga
     4201 aggatccagc ggatctgtca ctgcgatctg ccaggctccg ccctccggct tgtttccgta
     4261 agtcaggatg ctgccgccca cggaaatcac agcaccactt acctccggat gcgcttccag
     4321 tgtcttaagg atctcatcca gcgccacacc ttttcccaca gctcccagat ccaactgcat
     4381 ttctgcggga atgctcaccg aatccccgtc cgcctgttgt tccaactgaa ttttctgcca
     4441 gcctgtggtg gcaagagcct ccgcgatctc ttcctgcctc ggcagttcat aatccccgga
     4501 atctccggca gccgcccagg tatcaatgtc ccacagacgg ctgagccggc cgatactgac
     4561 atcaaaggcc ccggcactct cctctgagat ctgccggcat cttcccagcc actcagccat
     4621 cacaggagat acggaggtct gcccttctcc agcggctgca ttgatccgcg ccacttccgc
     4681 actttccagt ctccaggaaa gttctttttg ttccagatcg tttagttttt gtaaaagaac
     4741 atctgttatt ttctcattta ttttactatt tgttgtggtt acggaatttc cggtaacata
     4801 aacggtctgg gagatcacgg ttcccatggc ggtatctatg cttttttcac attccgtttt
     4861 ctgcccgcat cccgccagaa aaaacaccgg gagcattgcc agcaacgtcg cccgtcgcaa
     4921 cacccggggc gttttcaaat acttcttcat ttttttcatt ccttttgcta tactgttctc
     4981 atcagaaagg agttccttat tcctatgaat ctttccatga atgatcgaaa aaaagcagtt
     5041 ctccttacca ccgctctgct tgtaatagct gtcctttgcg tagtgcggat ctatgtagtc
     5101 tcgcacagtt ccgtagaaaa cggtcaggct ctctatgccg atctctatca gaacggcgaa
     5161 ttattgcaga ccatccgtct ggatacggtg accgccgaat atacctttga ggtctccggg
     5221 aatgccggtg ccacgaatac cgtctgtgtc cgtccgggca gcattgccat cgtctctgcg
     5281 tcctgtccgg atcagatctg cgtgcatcag ggatttatca gcacctccct gctccccatc
     5341 acctgtctgc ccaatcggct ggtgatccgg gtcagagaag aggtttccgt tccggatgac
     5401 actgcaccgg ttccggacgg cgtgacctac tgacgggata tcgtttttgc ctgcatgatt
     5461 ctaaccacac gatgccctaa tccacaactc gaaatcatcc ggtgctaacc acctgatgcc
     5521 aaacctccac ggtattaaag cccacggccc taaaacaccg cagatcttac gaattacgaa
     5581 aacacggaga ttttaatgaa accacctgta actacccgaa aactgacgac gctttctctg
     5641 ctggcggcca ttgcgctagc gctgtatgcc gtggaaagcg ctctgccccc cattgttccg
     5701 atccccggca ttaagctggg gcttgccaat ataataacgc ttgttgttct atggaaatat
     5761 tccg
//
LOCUS       DAWDHD010000163         5760 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_810_length_5760_cov_10.218230, whole genome shotgun sequence.
ACCESSION   DAWDHD010000163 DAWDHD010000000
VERSION     DAWDHD010000163.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5760)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5760)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5760
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_810_length_5760_cov_10.218230"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1269 a         1610 c         1283 g         1598 t
ORIGIN      
        1 tcgcatgctc cgggagttcg tggattttgc caatgtattt aactaacttt caattattcg
       61 caatacttca acaattccaa cgggtgggcg ataatagcat cggcgtggcc ttcttccagt
      121 tccttgcggt tgcggaagcc ccagaggact cctacagtga aggcgcctgc cgccttgccg
      181 gtcttcatgt ctgtagcggt atctcccatg tagagtactt cgtctgcggt gaaaccgaac
      241 tggtctaaga tgcggaagac tccggcggga tcgggcttga tgggcacgcc gtccacctgt
      301 ccctgcacta catcaaaata gcccttacca aataaactct caatgacatc tacggtggca
      361 tggtggggct tattggataa tacggcgatc ttcattccac gtttcttcaa ctcttccagc
      421 agttccggaa ttccctcata gggcttcacc tgatacatac agttcgcctg gaagatctcc
      481 ttgtatctgg cataggctgc ttcaaaatgg gtaagttccg tatcgccacc cttgatcagt
      541 gcccgcttta ccagattggc ggcaccgtct cccacaaaat atttataatc ctgtaccgag
      601 aaaggtccat agccgaattc cgccatggtc ttgtctccgc tgtatttcat gctcataata
      661 gaatcagata aggttccgtc caggtcaaaa attgctgctt tcttcattct gttctcctac
      721 actaatctca atccatgcag ttcctgatag actcttccca cgggaaggcc taccacatta
      781 ttgtaatctc cttcaatgcc gcggatatac cgtgcacaaa atccctgaat accataggct
      841 cccgccttgt ccatcggatc cccggtagcc acatattccc ggatctgtgc atcggtcata
      901 ggataaaaat gcactgtggt cttctcatgg aaagtcagcg tcttcctctc accgttttcc
      961 tcatagagga tcgtcacgcc tgtgtatacc tcatgtcctc tgccctgcag catagtaagc
     1021 atggctgccg cagcttccga atccgcaggc ttacccagaa tcttgccgtc gcaggcgacc
     1081 acagtatcgg ctcccagcac aagggtgtcc gtcatcgtag tttctccact tttctgttcc
     1141 ggatcacaga caccactttc cgcaagtttc tcccacaccg cttctgcctt ctgtgcggac
     1201 agttcttcca ccactttcgc aggctctgtg gatgtgatct tttcctcggt ctcgctgacc
     1261 actacatcga aatccagtcc gatctgtatc agaagctccc gcctcctggg ggaggcggat
     1321 gccagtatga tcttcttatt catgatgtgt ctccgggata aaagttctgc tttcgatggc
     1381 agactctccc gcagcctcct ttgctttttg aatggcctcc aggcagaaag catcctgaga
     1441 gttaaacagc tgctttcctc tcttgtcctg cttctcatag ctgccatccg gctgtaaaat
     1501 tgaagccttc acattatcct tcagttgact gttcaggata tgtagcaatt cctccttcag
     1561 ctccgggcga tctaccggga agaggatctc cacacgacgc tccagattac ggggcatcca
     1621 gtcggcactt ccacaataga tctcataatg gtcatcattt gcaaaataaa aaatacggct
     1681 atgctccagg aaattgccga cgatggagcg gacactgata ttctcgctga caccggggat
     1741 tcccaccttc aggcagcaga tacctctgac gatcagttcg atcttcacgc cggccgcact
     1801 tgcttcatac agggcacgga taatatcctt gtcgcagagg gagttcatct tcgcaatgat
     1861 atgagcattc tttccctcca gcgcataatt tttctcacgc tcgatcaggt gcaggaactt
     1921 atccttcagc cataacggtg ccaatgccag cttgttccag aacagtggct cagaataacc
     1981 ggacagcatg ttgaatactg ccgtggcatc ctcgccgatg cgctccgcgc aggtcagcat
     2041 acccacatcc gtgtacagct tcgccgtagc atcgttatag ttaccggtac ccagatggac
     2101 atatctgcgg atgccatcct cttctctgcg gaccaccagt gtgatcttgg aatgagtctt
     2161 taatcccacc agtccgtaga taacatgaca gcctgccttt tccagcattc tcgcccagac
     2221 gatattattt tcctcatcga aacgggcttt cagttccacc agtactgtga cctgcttgcc
     2281 gttctcggcg gcctgagcca atgccgcaat aatgggagaa ttgccgctga cacgatacaa
     2341 ggtctgtttg atagccagta cttccggatc tctcgccgcc tgacggatga aatccaccac
     2401 cggcgtaaag ctctggaacg gatgatgtaa gaggatatct cccttacgga tctcctcgaa
     2461 aatattgcag cctgtgggaa gctccggtga ctgctggggc tcatacttgg gagtctttaa
     2521 atggtcaaag ccctccagcc cgtagatctt catcaataca gtcagatcca acggtccgtc
     2581 gatttcataa atattctcgg gctcgatgtt caattccttc ttaatgatct tcagcagccg
     2641 cttatccatg ccgctttcca cttccaggcg gatggcttcg ccccactgac gcttctttaa
     2701 ctgcttttcg atctccttta acaggtctgc cgcctcatcc tcatcaatgc tcagatccgc
     2761 atttcgcatg atacggaaag gatgggcaca gacaatatca taattcagga acagcttatc
     2821 catgttccgc tcaatgatct cttctaacag aatgatcttg caggccttgc ccttagacgg
     2881 gatccgtacc acacggctca ggacactggg cacctgtacg gtggcgaatt ccagttcctc
     2941 gtcactgttt ttcttccgca ccatagctcc gatgttcaga gatttgttgc gaatcagagg
     3001 gaacggtctg gaagagtcca cggccatggg ggtcagcaca ggatacacat tttcctgaaa
     3061 atactgatcc acatacgttg cttcctccgc cgtcagctgc tcatgctctc tgacaatatg
     3121 cagcccgttt ttctccagaa gcgggagcag agaacggtta taggtggaat attgcagatc
     3181 caccagttca tggatcgcca catgcagcgc ttttaactgc tccgtgggag tcatgcccgc
     3241 aatgtctttt ttggtataac ccgcattttc catatccttt aaagatgcca cgcggatcat
     3301 gaaaaattca tccaggttcg atgctgtgat gcttaaaaat ttcaaccgtt cgaacagtgg
     3361 gatgctcttg tctctggcct cgttcaacac tctatagtcg aacagtaccc agctcagttc
     3421 ccggttggta taatatttcg gatctttaaa atcgatttcc attttgccct gctgcctccc
     3481 ttaaaatttc tttttctgcc ggatcaccgg ggtaatgctg tatacttcct tgaaaaattc
     3541 cgtgctggtc tggaagaatc ccttttccag cgtaatatct tcctgggtgt ccaccttcag
     3601 aatcagttca ttctcctgta aaacggcctt gacacctttc attttctgtt tgtggctgcg
     3661 gtcaaggctg ttggccagcc gtaagatggc agtcagtttg gcaaccgtta agtagctcgc
     3721 cttatccagt cccatagggc gttcctgtgc ctgcccataa tagacgaaat cgctgtggtt
     3781 aaaccttacc acgttggcca cgatctcccg ctccatatgg gacaatccga tcatttccgt
     3841 tgccatgatg atgtcatagg aagcctcgcc gacattgagc aggctgatat attttccaca
     3901 gtcgtgcaaa atagcggcca gccgcagata caggcgttct ctctgcccca tgccatgcac
     3961 ttttttcata ctgtcaaaaa tcgtggtagt gatatgttcc agggtatccg ctctcttact
     4021 gctgccattg tagcgcttgc tgatattcag tgcgcaggcg ataatatcct ccgcaaaatc
     4081 atgctcgcca cggaacattt tgttctgctc cgcatactca taggcgatac cgtcgcagag
     4141 tgttacgccc ggtgcccaga ccagttccgc gcccatgagt tctgcaattc gtctggtcag
     4201 aatggcactg atgtacacca gaggcacttt ctcctccgca atatccatcc gcttcgccag
     4261 ctccgtggtg cctttggtac gaagggcttc catcagctgg gaaaatgcct tgcgatccac
     4321 tacggcattg acttctcccc gctcgtgagc cttgcgcacc gcccagggag acaggtaatc
     4381 gtccacgatg atgatatttt tgatctcgcg gtccttgaga tacagcttct tgtaatcatc
     4441 cagctgcgcc gtagccagtt cgtccaccag acgctccatc tgagtgctgc ccgcgttcag
     4501 atgattcagc agctcctgca gccgcagtac ccccagccgc aggttctgcg tggagaccag
     4561 tgtatccttg tcgaacaggg aaatctggat actacccccg ccgatgtcca ggatcgccgt
     4621 cttttcctca atgattctgc ggaaagtctc tcccttggag gcaatggact tgtaatccag
     4681 gaaacgctgt tcggagttgc ttaaggtctc cacccggatg ccggtacgct gttcaatctg
     4741 atcctgtagg atcagggtat tttccgtctc ccggatggca ctggtaccgt aggctttgta
     4801 agctgtcacc ttgtaggatt ccatgatctg tgcaaattct ttcaaagtat gacacaggtc
     4861 atctattttc tcattgctaa tcttcccgtg ggcataggtg tccgagccca ggtcgatccg
     4921 ctggcggata cagtcgatct cccgcatggt atttttcccg gagagatcaa atattttcat
     4981 ggtgagttca aagctgccca catcaatggc tgcgaaggtt tttacactca taaggttcct
     5041 cactttccta acaggtaatc aatgaactgc tgggcacttc ttccggacag tccgccgtgg
     5101 gacagttccc atttgttggc ttccagtaaa agttcctctt ccggcatctc cacatgatag
     5161 cgctccgcca gagtcttcac gatattctgg aattcctttt tgttgggagc accgaaataa
     5221 atggtcacgc cgaaacggta taccagcgac agcttctcct gtacggtatc gctggtgtgc
     5281 atatcctctc tggtctcctc cttgtcactg tagttctcgc ggatgagatg gcggcggttg
     5341 gaagtagcat agatcaggac gttctccggc tttttctcca gaccaccctc gatgacagcc
     5401 tttaagtatt tgtattcgat ctcgaactct tcgaagctca ggtcatccat ataaatgata
     5461 aatttgtaat tgcggttctt gatctggctg atgaccgtgt tcagatcctg gaactgatgc
     5521 ttgtagacct cgataatacg caggcccctg tcataatatt cattggcaat ggctttgatg
     5581 ctggaagatt taccggttcc cgcatcaccg aacagcaggc agttgttggc ctttttaccg
     5641 tttacaaaag cctcggtatt gtctaccagt tttttcttgg gaagctcgta acctaccaga
     5701 tcatccagtt tcacatgggc aatgttcaaa atcggaacga tatgtacacc ctgctcgtca
//
LOCUS       DAWDHD010000164         5723 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_820_length_5723_cov_11.655963, whole genome shotgun sequence.
ACCESSION   DAWDHD010000164 DAWDHD010000000
VERSION     DAWDHD010000164.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5723)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5723)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5723
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_820_length_5723_cov_11.655963"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1606 a         1160 c         1502 g         1455 t
ORIGIN      
        1 ccgtctgggt acagatgaat tccattaaaa tggtaaagat gcagcctgag atcaaccgga
       61 tcaaggcaaa acattttggg gatgcagacc ggatcgcgga cgaacagtcc aagatcttca
      121 agagagagaa gtataatccg ctggcatccc tgatcccgct ggcagttcag atcattctgc
      181 tgatgggact ggtagaggtt atctatcacc cactggatta tctgcttcat ctgccacagg
      241 atgtgatcac ggcattcaac ggtctggctg tcagcctggc cggagcaaat ccggagagct
      301 cctctattca gctggcagta gtggagatga tcaagagtgg caattatgca gaacagttcg
      361 cagcattgca gtcaggactg gcaggggtgg acattgcgtc tgtattacag caggtacagg
      421 gaatttccct gaacttctgc ggcatgaatt taagttgggt accttccgaa gtggggggca
      481 ttgatatcat cgtccccatt gcagccggta tttccgcatg gctgttgtgt gtggcacaga
      541 atgcagccaa tgtcattcag gcagagcagt ccaagctgaa caaatacggt atgatggcat
      601 tttcggtagg cctgtccctg tatctgggct ggtttgttcc ggcaggtgtg gctttgtact
      661 ggatcgccag caacctgttc gcaattttac agcagtatct gctgaactgg gccatcaatc
      721 ccaaggatta tgtggattat gaggaactgg aggccagcaa acaggaactg gaggaattgc
      781 agtccattgg tggcaagaag aaactcttcg agaaaaatcc ctatgccaag agagagaaga
      841 aagatttcaa acgtttcttc tctgtagtga acaagcatct ggtattttat tccgagagca
      901 gtggcttcta taaatattat cagggcatta tcgaatggct cctggcacat accaatctga
      961 ctatccacta tatcaccagt gatccggaag atcagatctt tgcactggcg gaaaaagaag
     1021 acaagatccg ggcatattat atcggagaaa aacgtctgat cacactgatg atgaagatgg
     1081 atgcagacgt agtggtcatg actatgccgg atattgagaa cttccatatt aagcgcagtt
     1141 atataaggaa ggatattgaa tatatctata ttcctcactg tatggacagt ctgaatatga
     1201 ccatgcgtac cggttccatg gatcactacg ataccgtata ctgcgtcggt aagcatcata
     1261 ctgaggaaat ccgcaagaca gaggaagctt acggtctgcc tcccaagaag ctgatcgact
     1321 ggggctactg cctgctggat cgtatgatcg aagattacag gaaagcggat aagacgcctc
     1381 atgagaaaaa gcatatcctg atcgctcctt cctggcagaa ggacaatatc gtggacagct
     1441 gtctggaagg catgctggat gatctggcag gtaagggtta tgaagtggta gtacgtcctc
     1501 atcctcaaca ggtgcgtctg cagcaggata agatggatcg cctgaaagag cgttatgcca
     1561 agaacccgga tattgacatt cagacagact tctcatccaa cagcacggta ttcgaggcgg
     1621 atctgctggt gactgactgg tccggtatcg cttatgaata tgcctttacg accaagaagc
     1681 cggttctgtt cgtggatact cccatgaagg ttatgaatcc ggaataccag aagatcgaca
     1741 ccgtacctat caatatctgg atgagggatg tcatcggaga ccgtctggat cctacaaaga
     1801 cagaggagac cgaaagcaag gtcagaaatc tgctggagca gaaggatgct taccatgaca
     1861 gaattgaaaa gttcgtggaa gaatatgtat acaatctggg caacagtgcc gaagtaggtg
     1921 caaagtacat tgtacaggca gtccaggaac agattaaaaa agcaaaagaa caataaaaat
     1981 ggaaatacaa atagagtaaa agaaagaggt aaaaacatga aaaaattaag caagatcgca
     2041 caggtaacat tttttgcagc agcattcgca gtactgtttt ccggaacagc agatgcatat
     2101 attgatcctt ccgttatgaa ctatgctatt caggcagtag ccggtgtagt catcgctgta
     2161 ggcgctgcag ccggaatcta ctggagaaaa gccaagaaga agatcgacaa gagcctggga
     2221 atcgacgaga acagaaacaa agaagtagaa tccgatgata ttatgctgga tcaggcagag
     2281 gacaagcagg agaagtaatc tgacatgaaa aagcgggata aaaagcaact gctgccggga
     2341 atcgtggtgg cggttgccat cagtttcctg gtgtttttat atgcaccggt agacttgtat
     2401 tgcagtaata tatccgagtt ctggtttgat ttcggaattc tgatcaaggc atcattgggc
     2461 ctgtttttga tcagcaccgt tgtgctgctt ctgatctata ttgtcttgtg gctgatccac
     2521 ccggtgctgt accgtctcgg actggcaggc ggcttcttag ggctgatctg tacctatatt
     2581 cagggaaatt ttctgatcag gaatctgccg cccctggatg gaacgacgat tcgatgggaa
     2641 gaatatacat ttctgaggac agaggatttt attctctggg gcatcggctt tgttgttgta
     2701 gtgctcatgt atattttcct gaaaaaagat cttttgtcca aaacggtaat gtatttaagc
     2761 ggcggattga cattgatgct gctgatcaca gcagtcagta ttgtatttac cggtggtgca
     2821 ctgcaggaaa aagtgcatta tcaatatggt gcggacgaac agtttgtgat gtcagaagac
     2881 cagaacttcg tgatccttct gctggatacg gtggattcca gaacgatcac ggagctgttg
     2941 gacgagcatc cggaatacag tgaggtattt cgtgatttca cctattttga aaatacggta
     3001 ggagcatact cctgcacaga gagatccgtt ccctatattc tgtccggaga ctggtatgaa
     3061 aatgatgaac cctttgatga ttacggcaag agggtgtacc aggagtcccc actgtttcag
     3121 acattgcagg aacgtggcta caatatggaa ctctgtgatt ctgaattgta tatggatacc
     3181 gaactgatgc atatgttcag caacattcac gtagtggatt tcgaactgag ttcttatacg
     3241 aaatttgcga aaccactgtt gaagctgatc ggtttccggt atgcgccctt tgaactgaag
     3301 aaaatgtgta catttaaaaa ggctgcattc gcagaactgc tccaggtaga gaatacacct
     3361 gaggaaacca gctgcagtga aacggatcat gtatttaaga gccagctgga tctgcgggga
     3421 attaccgtgg aagattcctc caaaaaattc aaatttattc atctgaacgg agcacatgtt
     3481 ccttatatct atgataagga tatgaatatc atcaatgaac tggacggcac ctatgaacag
     3541 agcgcacagg ccacgatggt cggtgcaatg gattatgtcg aacatctgcg caacagtgaa
     3601 gcctacgaca atacagtcct cattgtgatg tcggatcatg gctataacgg aagcctggga
     3661 caaagtggag aggctacctg gatgcgtcag tgtgcattac ttctgatcaa gggaagaaat
     3721 gagcatcatg ataccatgca gatctcacag gcacccataa gctttgagga tctccaggag
     3781 gcatatgtgc gtctgctgga cggcaggcgg agtgatgagg tctttgactg gaaggaaggc
     3841 gatgcaagag aacgccggtt cttacggtat tctttcctgg atgacgatca tatgcaggag
     3901 tatatacaga cggggtatgc ttctgatatg gataccatga ttcctaccgg aagagagtat
     3961 aatcgttaga aagaaatgca acaatgtgac agggagaata agtgtgagta aaaatactgt
     4021 gagaaaaaag ataacaggca gacagctgat gccggggatt ttcatatccc tggctatcag
     4081 ctttttgttg tttttatatg cacctgtcga tctgtactgc gcgaatgttt cggaattctg
     4141 gtttgatttt tccacgttat tgatcactgc actggggatg tttgcagcct gtttcgccgt
     4201 actggcggtg ttgtatctga ttgccatgct gattcatccg tatgtgtacc ggatcacact
     4261 ggccggagga attgcacttt ttatctgcac ctatatccag gggaatttta tgatcgacag
     4321 actgccgtca ttggacggaa cttcaatctg gtggggcaaa tacgatattc tccggaaaga
     4381 tacattgctt ctttgggtag tagtgctgat cgttgtaatt gcggcaatga tcgtgctgcg
     4441 aaagcagaaa tttgtccatg tggtaatgtt catcagcgga tgcatgaccc tgatgcttct
     4501 ggttaccgca tgcagcaccg tgattaccag cggggcactg cattccaaac tgcatctgca
     4561 tgtcagcgtg gaagaagaat tcgagatgtc tgcggacaac aatttcgtga tccttgtgct
     4621 ggatacggca gattccagag agtttacctc actgttagag gaacatccgg agtaccggga
     4681 gatctttgga gattttactt attttgagaa taccacggga gcttacacca gcacattgaa
     4741 tgctattcct tttattttat ccggtgaatg gtatgagaat gagggatctt ttcaggacta
     4801 tctggacaga gtctacagag aggcaccgtt gtggaaggaa ctgaagagca gaaattatca
     4861 gatcgatctc tatgaggatg atatccgtgc acaggatgct tccatcgtgg acaattttga
     4921 caatgtctat tttactaagg tcacccccgt ttcttatctg gaactggcaa agcaggagct
     4981 gaagctcgtt ggattccggt atgcaccgta tgatctgaaa agatactgcg tgatcaaaga
     5041 agtctatttt aatgaattac gggaatctca tccggaaggt tcggattcca aggcgtttac
     5101 tgcggataat acggctttca aggaggatct ggcagcccag ggtgttgtga tggatgagac
     5161 gggaaaccgt ttcaagttca ttcatctgaa cggggctcac gcacctttta tctatgacaa
     5221 ggatgccaat gtcatcggtg tggaacaggg atcttaccgt cagagcatgg aagcttccat
     5281 tacattggca gcaaattata ttcaggcatt gaaagacagt ggtgcctatg acaacaccgc
     5341 tctgatcgta atggcggatc acggatataa cggcatggga gaaaaggaag aggactttct
     5401 gcggcaggca gcgctgcttc tgatcaaagg aagaaatgaa cagcacgata ccatgcagat
     5461 ttcagaagca cccatcagct acgaagacct gcaggaggcg tatgtccgac tgctggatgg
     5521 cgcgcagagc gacgcggtgt ttgactggaa agagggagat accagagaga gacgttttct
     5581 ggaatacgct cccggcagcg agaaacacat ggtagaatat ctccagaaag gccatgcaca
     5641 ggatctgaca actatggtgc ctaccggaag ggtgttcgac cggtaataac ttttcctgcg
     5701 atagaagcgg cggggatgat ccc
//
LOCUS       DAWDHD010000165         5718 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_822_length_5718_cov_10.249161, whole genome shotgun sequence.
ACCESSION   DAWDHD010000165 DAWDHD010000000
VERSION     DAWDHD010000165.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5718)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5718)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5718
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_822_length_5718_cov_10.249161"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1560 a         1300 c         1603 g         1255 t
ORIGIN      
        1 tgccggacgg ctttttgcgt aatgtgatag atcaaatatt attttttctg gtttgcaggc
       61 tccgaaaagc cttgtattta cagtgttcct aataggaagt aatcatatgt ttgtgagaag
      121 aactctgtga ggagcttcgg tcgaaacgga acaatccgta ggcatcaaag tcggggactt
      181 ttggccccgg catcataata tgctgactat atagaaatag tataaacgaa gtccgccgaa
      241 aagggaatat gaatattttg tgtaaaggct ttacgaacgg aatgtttcgt gttattataa
      301 gtagtattaa aaactacata gtatggtttt tgaaaaatga aaataattgt aactattcag
      361 tgccccattc gcagaatgac tctttgggag ttatattttt gaatagctgc aatcaatgat
      421 atggagatta atatgagttt taaaatcgta gtagacagct gttgtgaatt gccggaagaa
      481 tatttgcagg attcccggtt cgagatcgtg cctttgggac tggaagtggg agattaccag
      541 attcaggatg atgagaattt tgatcaggcg gaattcctga aaaaggtggc agagtgtccg
      601 aaatgtccca agtcggcatg cccttcccca gagcgtttca gggaagccta ccacacagaa
      661 gcagagcatg tgtatgtgat cactttatcc tcccacctca gcggaagcta caacagtgcg
      721 gtgcttggta tgaatctgta tcgggaaaaa tacgggaaga agcagatcca cgtcatcgat
      781 tccgagtccg ccagctgtgg tgagacccag atcgtgaaga aactggtaga actggaagag
      841 cagggccttt ccttcgaaga aatcgtgaag cagatcgaag agttccgcag caatgtgcat
      901 acctattttg tactggataa tctggagacg ctgcgcaaga acggcagact gaccggtgtg
      961 aaagcactgg tggcatccac gctgaacatc aagccgatca tggccggaga caagggcagt
     1021 atcgtgcaga gaggccagag cgtcggcatg aagaaagcac tccgaagaat ggcggagatc
     1081 gttctggaag aggcagggga tacgaaggaa cgtttcctga tgatcaccca ctgcaatgct
     1141 cctgaccgcg ccgagaccat gaagaagctt cttatggaaa aggcagagtt caaaggatgc
     1201 ctgatcatgg acacccgcgg tgtcagcagc atgtacgcca acgatggcgg cgtgatcgtg
     1261 acggtgtaaa aagattaata tcataggcaa tatagctccg gcggattagc ctgtcatttt
     1321 ttgtttctga cagcctgttc cgcctgggct atttttttca taaaaagcaa atatagaggg
     1381 cattttatga aaaacagaat ttgagagagc ggtgccatga atctggcaaa ataagaaatt
     1441 gaaatgtaag agcttacaca cacgtcagac aaacatcctc atgcatattc cacaaaaaga
     1501 tagataaaaa attaactttc ctaaaaacta ttgatagttt ttattattta agatacaatt
     1561 gcacatatac aaaaatcagt agccgacaag aaacggctat tttttaaaaa acaaaatgta
     1621 agcgcttaca cggcgcaaca ggcagccagc tcaggtggcc aatgccatag tacgtgtcag
     1681 taaaacaccg cacccgcaga cggagagcag cacgccatag ccgtggccgg cagcgcccgt
     1741 ggcgcagggc ataaaagtaa cgcaggcatc agcccgcaga atggagaaga ttatgagccg
     1801 attgaagata gctaccccga ataaagcaca gcttacggtg gaacgcctgt acaaagatct
     1861 ggagagaaga attatcgcca gcccgcccgg attatgtccg gtggacctgc agctttcttt
     1921 tctgaaaatg tgtcatgccc agacctgtgg taaatgtgtg ccctgccgtg tgggactggg
     1981 gcagttgcag aacctgatgg aggatgtatt ggcaggcaaa gccaccctga agacactgga
     2041 tctgatccgg gataccgctt ccgacattgt ggactccgct gactgtgcca tcggttacga
     2101 ggcggcccat atggtactgg ccggactgga aggcttccgt gaggattatg tctatcacat
     2161 tgaacatggg ggaaaatgtt cctgccatat tactcagccg gttccctgtg tggcgttgtg
     2221 ccccgcaggt gtggatatcc ccggttacat agcactggtg aaggaagaac gttatgcgga
     2281 tgccgtgaag ctgatccgca aggacaatcc tttccccacc gcctgtgccc tgatctgtga
     2341 gcatccctgt gaggcaagat gccgccgcaa catgatcgac agtgccatca atatccgcgg
     2401 cctgaagcgt atggcagtgg acaatgccag agccaacacg gttcccgtgc ctgagaaagc
     2461 ggagagcacc ggtaaaaaag tagcaatcat cggcggaggc cccggcggac tgtctgcggc
     2521 atattatctg gagctgatgg gacaccatgc cgtggtattt gaggaaaaga gcaagctggg
     2581 cggtatgctg cgctacggaa ttcccaacta tcgtttccca agagaacgct tacaggagga
     2641 cattgacact attctgtcca ccggagtgga agtgaaattg aacaccagag tcggcaacgg
     2701 cgaaggtgag atttcttata ataagctgca cgaagaatac gatgcagtct atattgccat
     2761 cggtgcccat acggataaaa agatcggtat cgagggagag gatgccaacg gtgtcatgtc
     2821 tgcagtggaa atgctgcgcc gcatcggtga cgatgacatg ccggacttca aggacaagac
     2881 cgtagtcgta gtcggcggcg gtaacgtagc catggactgt acccgttccg ccattcgtct
     2941 gggtgcgaaa aaggtgggaa ttgcctaccg cagaagacag accgatatga cggcgcttcc
     3001 tgaagaagtg gaaggcgcca tcgcagaggg cgcagagctt tacagcctga aagcacctca
     3061 caagatcgaa gccgacgaga acggcaatgt gacggctctg tgggtagaac cgcagatcat
     3121 cggccccatc gatgcctggg gacgagcccg tccgatccag gcggatgtgc ctctccttcg
     3181 aatcccctgt gatatcgtcg tggtggccat cggtcagggt atcgagaccc gccatttcga
     3241 ggaggcgggt atctccgtaa aacgaggcac cattactgcc atgagtgaca gtgaagtcaa
     3301 ggatgtccac ggtgtatttg ccggtggtga ctgtgtgacc ggtccggcta ccgtcatccg
     3361 tgccattgcc gccggaaaag tagctgcagc caatatcgat gaatatctgg gttaccacca
     3421 tgtagtaccc tgtgatgtgg agatcccgcc ggtatcctac gaggacaagg aaccctgcgg
     3481 acggatccag ctgagtgaaa aggaagctgg agagcgcaag acgaatttcg atgcatttga
     3541 atgcggcatg acgaagcagg aagcctgcca ggaagcgggc agatgcttac gctgcgataa
     3601 atacggctac ggaagcttaa agggcggccg agctgccatc tggtaaaaag aggagcaaac
     3661 aatgggcgag aaaatgataa accttacaat agacggcgtg cagctgcagg tcccggaggg
     3721 aaccagcgtc atgagcgcgg cagcaggcgt gggaatcgaa gtaccgcatc tgtgcttttt
     3781 aaaagacatc aatgagatca gtgcctgcaa ggtctgcgtg gtggaagtgc agggaaaaag
     3841 caagctgatc acagcctgca acagcccggt ggaagaggga atggtagtgt ataccaattc
     3901 tccgaaggta cgccgtgtgc gcaagacaaa tgtggaactg atcctgtccc agcatgattg
     3961 tcactgtgcc acctgcgtca gaagccgtaa ctgtaattta cagcagattt ccaacgatct
     4021 gggaattctg gaagtacctt ttaccgagga agttcccgag acaccctggg atcattcttt
     4081 ccctctgatc cgggattcca gaaaatgtat taaatgtatg cgctgtgtac agatctgcga
     4141 taaagtccag gccatgcatg tctgggatgt acagaacacc ggatcccgta ccacagtaga
     4201 tgtggcggat aataagacca tcgattgctc cgactgtaca ctgtgtggac agtgtattac
     4261 ccattgtcct accggagcat tgcgggaacg agacgataca tacaaggctt tcgaggcact
     4321 ggcggatccc gagaaggtca cagtagtcca ggtggcaccg gcggtaagaa ccgcatgggg
     4381 cgaggaactg ggactgaatg cggaggaagc ttccgagggc aaaatggtgg cagcgctgaa
     4441 gcgcattggt tttgactatg tatttgatac caacttcgca gcggatctga cgattatgga
     4501 agagggaaac gaactgctgg atcggctgga caattcccgg aaatatgcat ggcccatgtt
     4561 tacctcctgc tgtcccggct gggtaagctt cgtgtccaag aagtatccgg agtatctgcg
     4621 gaatttgtcc acggcaaaat ccccccagca gatgttcggt gccatggcga agacttattt
     4681 tgcacagaag aaagggattg atcccaataa tatctgctgt atctccatca tgccctgcgt
     4741 atccaagaag agagaggctt ctctttctta tatgaagagc gccggagccg gacaggatgt
     4801 ggatattgta ctgaccacaa gagaattcgt gcggatgatc cgtgccgagc atatcaatac
     4861 ccggttctta aaggagcagg cttttgattc tcctctggga gaaagcaccg gtgccggagt
     4921 gatcttcggt gtcaccggcg gtgtcatgga ggcagcactt cgtaccgcat atgcagtagt
     4981 ggaaggcaag aatccggaag cagatgcttt ccgggcagtc cggggaagag acggcagaag
     5041 agaagcagac ttcacactgg gagatcagat cttacatacc tgtactgtca gcggactggc
     5101 caatgcggag aagctgatgg aggacattaa ggcaggaagg gtatcctatg atttcgtgga
     5161 agtcatggca tgccccggtg gttgtgtcgg aggcggcgga cagcccatcc acgacggatg
     5221 cgaattcgcg gagcggcgcg gaggcaccct gtaccgtctg gacagtgaga gaaagcttcg
     5281 cttctcccat gagaaccccg aggtacagaa ggcctacgaa gagttcctgg gcaagcccct
     5341 cagccgcacc gcccataagc ttttgcattc cgaacacgtg aacgaactgt actttgaaac
     5401 acataactac ttgtaatacc aggtgaccga ttcagccgca gggcatgctt gcatgcgcct
     5461 gcggctgaat cagggaaccg cccaaacatg aggaagaagc gatttgcgaa gcaaatcagc
     5521 ggaatccgaa tgtttgcaga attgcgaaag ctgcacggca gcggagcaat tcgtgggtat
     5581 tacaatgtat gggcatgctt gcatgcgcct gcggctgaat cagctgaatc cgaatgtttg
     5641 caagacccta ttgacatttt ttcacacagg gtctatactg tgtatagttt aaagatgatt
     5701 actagagtaa gagtggac
//
LOCUS       DAWDHD010000166         5670 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_830_length_5670_cov_8.558682, whole genome shotgun sequence.
ACCESSION   DAWDHD010000166 DAWDHD010000000
VERSION     DAWDHD010000166.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5670)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5670)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5670
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_830_length_5670_cov_8.558682"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1285 a         1441 c         1210 g         1734 t
ORIGIN      
        1 tctaccaggg agaaaccttt gtttgttaag ctcttctgtt tcttgttcat aataacattc
       61 tcctttttat gtgtttttta atttcagggt agtaagtttt ccgccccatt tattaattat
      121 cgttctgtcc ctacacagaa cggataatcc ctttttattt acatagcatc cagtgcttca
      181 tacatggaca tcataggcat catacatgcc gccagcaatc caccgactac aacagccagt
      241 acaataatga tcataggttc caatgctgcc atgagggact gcacttccat ttccacttct
      301 tcctcataat aatcggcaag tttgtccaac atttcttctg tattaccgga ttcttcaccg
      361 atacgaatca tctggtacac catcggagga aacaatccgc tttcctgtaa aggtctggac
      421 agcggcatac ctatagtaat ttcttccttg gcatcttcta atgcctcttt gaaatataca
      481 ttactcataa ccccggatac aatatccact gcctcaatca gtggaactcc ggcacccatt
      541 agtgtactta atgtacgagc catcagagaa gacgacgcct taatcgtcag cttgctaaac
      601 agaggaatct tcaaagcaat ttttccaaag aaatgtttac caatatctgt tttagcaaac
      661 gctctgattg caaatacaat agctacaata accggcacaa tgataaacca gtagttgatt
      721 atgccatgac tcaaattcac atagaacttt gtaatccagg gaagttctcc gcctatctgg
      781 ctgaacatat cctcatagga aggaataact tttaccagca ttacaatggt aactatgatt
      841 gcaactatac acaccgcaat cgggtaaatc attgcttttt taaccatcgc ctgtgtctta
      901 tgtgactttt ccagctgtgt agctacacgt tccatagccg tttccagact accagatgcc
      961 tcacccgcag ccaccatgtt caccatgatc gccgggaaca ccttcggatg ctctgccatg
     1021 gaatctgcca gggtctcacc cttttccacg ctgatgcgga cttccttcag tgcgtcctgt
     1081 aatctcttgt tctctgtctg ctcgcagagc attttcaggg attcgaggat gctgacgccc
     1141 gctttggtca tgctgacgaa ctggcggcac atgacgctta gatccctggc cttgggccat
     1201 ccgccgatct ggatgtcgat gtctttcgtc aggaagctct gttctcccag atcgataacg
     1261 gtcagctcct gccttctcag ttctgccatt gccagatctt tgttatccgc ttccaaactt
     1321 ccctttactt cttttccagc ctttgtgatg gctctgtagc cataggtcgc catgtgcctt
     1381 ccctccaatg cgtctctatc tttaactttc tccccaacca tgtaagacga gccccggtcc
     1441 tgaaaaggtc caggtttttg aaaaattttt ataaattttg ataaatttct ataaaatatt
     1501 gcggttttta tagaattata cgtcaaatga tacctttacc atctcggaga aggaggtggt
     1561 gccatccagt acatagtccg tggcactcat acgcagggta tgcattcctt cttttaatgc
     1621 ttcttctttt aagatatcgg caccctcccg cttgctgata atggttttca gtttcggagt
     1681 gatctgcatg atctcataga caccgatacg gcctttgaag ccggtattgt cgcatttgct
     1741 gcagccgcag ggctcgtaga tggttacgtt ttcctcttcc ttcagcccca tgaattcttt
     1801 ttcatccttg gtggcctgct tcggcttctt acagacgggg cagagtctgc ggaccagccg
     1861 ctgtgcgata acgccgatga cggaatctgc gatcaggtag gattcgatcc ccatatcctc
     1921 cagacgggtg attgtgctgg cggaggaatt ggtatgcagg gtacttacca ccaggtgtcc
     1981 ggtgatggac gcctgtacgg caatgctggc ggtctcctgg tctcggatct caccgatcat
     2041 gatgatatcc gggtcctgtc gcaggatgga acgcagtgca gatgcgaagg tcaggtctgc
     2101 cttggtgttg acctgtacct ggttgatacc gtcaatattg gcctcgacag gatcctcgac
     2161 ggtgatgatg ttcacgtctt ctttattcag ttcgttcagt gcggtataca gggtggtgga
     2221 cttaccactt ccggtaggtc cggttaccag gatgataccg tgaggatttt tcagaatgta
     2281 gtcaaactgt tttaattcgt agggtttgaa gcctaactgg ctcttgtcgc ggttcagtgc
     2341 actcttcgct gcaagacgca ttacgacttt ttccccgtat acggtaggca ggatggatac
     2401 acggatatcg tattccacac ggtctaccac ctgggtgata cggccgtcct gcggctttct
     2461 cttctcggaa atatccatgc cgccgataat cttaatacgg gcgatgatgg cagacaatac
     2521 attgacaccg tatcttgctc tctcatacaa tgcaccgtcg atacggtaac ggacacggat
     2581 gatattttcc atgggttcga tatggatatc ggatgcacgc tggcggactg ctttttcgat
     2641 catgtccttg accagcatta cgatagggga actgttgatg ttctcctccg tggcgatgtc
     2701 ttccaccaca tccagctttt tctctttggc ataggctgcc agtgcctcgg tcacttcctc
     2761 ctgaccgaaa tatttatcga tggccagcat gatgctgcgg gtggtggcta ctacgggctc
     2821 tacctggaag ttggtgatga tgttgatgtc atcgatggcg ttgtaatcca ggggatccgc
     2881 catggcgacc atgagaacgt tgaagttatt ttctttgtag gcgaagggga atatcttgtt
     2941 cttcttcagg acgttgaccg gtaccagatt cagtacttcc ttgtccacat tgacgctctg
     3001 gagatcgatc atatccagat ccagctggcg gctcagtgcc ttcgccagat catcctccga
     3061 tacgatgcct tcatctacca ggaattctcc cagcttcttg ccctgtcggg cgggattatc
     3121 caacgcctgc tgcagttgct cattcgtgag gacaccgctg tttaccagta attcacctaa
     3181 acggaccttt tttctgccta attccataaa ttctcacctg ttactcttat cgtcattctt
     3241 atcgttactt ggaaatatgt gtctaattgt agcattttta gctacaattc acaaatattt
     3301 tgtctcaata cggaaatatt atagcatatt ttagatttat gtaaattgtt tttagtactt
     3361 ttgtttcaat ttctgagaaa aacatttttg ttgctttctt tcctgttaca cagtacaatt
     3421 atttcatttt attttgaaag tttctaaata cgccaattct tcggcgaatt ttcccaacat
     3481 ttattgactt cacgtattat acgtgttacc ttaagaaagg agagataatc catgcgtttt
     3541 cgcgaattag agcgaatcat ccttgacgat ggctgggttt tgtctgatat cagaggttct
     3601 catcatcagt acaaacatcc ttcaaagtca gggaaagtaa ccattcccaa tcacagcggc
     3661 gatattcctt tgcgagttgt taattctatt tttaaacagg ctggactaaa ataatatgga
     3721 acagcctttg aaaggagtga tcttatgaat tatatatatc cggctatttt ttacccggaa
     3781 gcgaatggga aatactctgt catttttccc gatcttaatg atctcgccac ctatggtgac
     3841 agtcttgcag atgcctttgc catggcacag gaagcatgtg cacaatatct gtttacttct
     3901 ctgagagacg gcgatgctct tcccgcccct actgcaatta atgcagtgaa atcagatgat
     3961 cccactgcat taataaatct aatctgtgtg aatttggata attatgcccg tgcttatgat
     4021 gacagggctg ttaagaagac actgactatt ccggcgtggt taaacactgc ctgtgataat
     4081 tgcggtatca attattccaa agttctaaag gacgctctca ttgccaaact gcaacaagtg
     4141 caaatgtgac cgtatcacaa agtcaccgcg tattcactgt cgtacatttt cttcagattt
     4201 tctacgctgt tatatttttc gccgatgcag aattccttgg accaggtcat atagtaggcc
     4261 caggggattc tgcttttttg caggagatcc acatcgggaa ggtagcccac ttctgccagg
     4321 gcagccactt tgtcacggga ggtggctgtc accagtttct cataggcatc cgcatagtct
     4381 gtggactgat actccgggag gtagacatcc atggatacta catccacgta ttcatctccg
     4441 gggtaggctt ttggtatatc gctgttccat acccagagga ggttatgcag gtctttctca
     4501 cctgtgtagt aatcgaacat caggcggtac agctctctcg ccacttccgc tcccttcgct
     4561 ccccaccaga accaggtacc gtaggattca tggaacgggc gccagagaat gggaatccgc
     4621 tcttcctgaa agcgccgcag gatctccgca atcacatcca tgtcatgata gaatgccact
     4681 ctctctgggg ttccctcttt caatacttcc cttgcatcaa aatccgtgtg ttcggtatag
     4741 aagctcttgt ctctgccttc cagaggcgag aaccagtgga aggtgaaggt cagaatgcca
     4801 ccgttaccat ttttgcgttg ttctatcgcc cactgcagtg ccatctctgc ggtgccttta
     4861 ttttcttcta tttccgtcag acattccggg gaagcgtctg cataattgat attcggagaa
     4921 taccccagtt gttcaaagcc acgcagcttt ggctcttttc cggtgggctg ccggatttat
     4981 gcaatctcct cacaggggat ggtctgggtg tgctgaccgg tgatgatctt ttttcctgcg
     5041 acgcaatgaa gctcccgcag cagctcctgc gcttcctttg ttgcctctga atccgctgtt
     5101 gtaatatttt tctcgtccat atatacacct ctttctcctc catacccgca cttacgactt
     5161 ccccgggtat acaagcttgt tttttctctc catgcccgcc gccacaaaaa aatcgagggt
     5221 acagagacaa tctttccctc tctgtaccct cagtttaatg caatacggaa cgaatcgcaa
     5281 gctctattcc ggctttatac ttcctgataa tcgttaccgg tgtcgttctt ctctactggc
     5341 tttgcatctt ctgccttttc cttggcaaag cccttcgcca gcatctcgtt gatatcgagt
     5401 ccggtggact ccttgatgcc gtcggtgacc tgggtcacgg tggtcatgat atcgcctgcc
     5461 agcttgctgg aatcgccgcc gaacatcttg atggaatcta cgttcatgta gcccttagcc
     5521 acagcctcgg cgatcttggg cagctgctcg aagtatacct tcagagtatc taactccatc
     5581 tgctgtcttg cagcgtcgcc gtactgcttc atagcttctg cctttttcag gataccggct
     5641 gcctctgcct ctgccttcgc ctggatagct
//
LOCUS       DAWDHD010000167         5623 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_836_length_5623_cov_10.321121, whole genome shotgun sequence.
ACCESSION   DAWDHD010000167 DAWDHD010000000
VERSION     DAWDHD010000167.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5623)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5623)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5623
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_836_length_5623_cov_10.321121"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1331 a         1493 c         1205 g         1594 t
ORIGIN      
        1 tccacgcccc gcagtttgcg gatgcccgcg tagtactggg gctgataata cagcagaaaa
       61 ttattctcaa aaacagcctc ttttaatttg ctgtccagtt ctacattttt caggaaatcg
      121 ttcaggatcg gcgtatcgaa atactggatc cggttcttgc ccatggcttt tcccttgaac
      181 attacgattt ccgcacagtt gatcagttcc agtgcggaag tcgcagcctc cggatattcc
      241 gccacaccga cacttacggt gatgttcaga acctgcccgc ccaccagata aaacggctcc
      301 ctggtgcgct taacgatctc cttatgaata tgttccacac ttttgttccc acaaggatca
      361 taaatggcca tgcagtagac atcactggtc aggtggcaga cgatcacatc atccccgtta
      421 aattccttca gaaaagagcc aaactgctgt accagttcgt cccccgccac gattcccagt
      481 ccgtcattta ccttgcggaa atcatcaata tcaataacaa gcacgctgac cagccggtta
      541 tcctctttgg cccgacgaag aaattcggtc agaagacgca caaaataatt tcggttgtac
      601 agtccggtca gactgtcgta atacgccatg tagagaagct cttcgttctg tgttttctgt
      661 ttggtgatat tctgtaccac aataatctga tccgtcggcc tgccatcttc gtagaaaatc
      721 ctgctggaaa actgcaacca ggtcttcttt cccctctgca tacattccac agagtccgtc
      781 tcccgaccgc tcttttccaa aaacagcatt tcccgaagtg ccaacacata cggttcgtcc
      841 accatctcct gaagttttac aaaatctctt ctgtccagaa tctggaagtc aaagaaatca
      901 tcccattttc ccagcgtagt gaccgtattc ttttcaaaag aatagtagat ataagcataa
      961 tccatggtat cgcacaacag acgatacatt ttctccttag cgctcagttt ctgattcatt
     1021 gcgcgcataa gatccagctg atagcgtaac tcgttcataa ccccactcac cctttatttt
     1081 ttggtgatgt aaccctgtgc tttcaaaagt tctgcgcaaa gcaatgctcc cccagctgct
     1141 ccacgtaagg tattgtgtga caaacctacg aacttgtaat catatacggt atcttctctc
     1201 agacgaccta cactgatacc cataccgttc tcgaaatcaa catccagagc cacctgaggt
     1261 ctgttgtcct cctccagata ctggataaac tgcttcggtg cgctgggaag tcccagtctc
     1321 tgaggctcac cgctgaaagt gaccagtttc tcgatcagct gctccttggt gggcttctta
     1381 cggaatttaa caaatacggc tgcggtatgt ccgttcaata ccggaacacg gatgcactga
     1441 caggtgatca cgggagaagt tgcgggaacg atcttatcgc cttccacttt accccagata
     1501 cgtaagggtt ctttctcact cttctcttcc tcaccaccga tgtaaggaat gatattgcct
     1561 accatctcgg gccagtcctt aaaagtcttg cctgcgccgg agattgcctg ataagtggtt
     1621 gctaccactt cgtaaggctc gaattccttc catgcggtca gaaccggtgc atagctctgg
     1681 atggagcagt tgggctttac agcgataaat cctctggtgg ttcccagtct cttcttctgt
     1741 gcctcgatga tctggaaatg ttcgggattg atctcgggta ctaccatggg aacatcgggg
     1801 gtccatctgt gggcactgtt gttggatacc acaggggttt cggtctttgc gtatgcttcc
     1861 tcgatggcct tgatctcatc cttggtcata tctaccgcac tgaatacgaa atcaacctgt
     1921 gcggctactt tttctacttc attgacattc atgactacca gctttttcac agcttcgggc
     1981 atgggagtgg tcatcttcca acggtcaccc acggcctcct cataagtctt gcctgcggaa
     2041 cgggggctgg cagcaatggt cgttacctcg aaccagggat ggttctccaa cagagagata
     2101 aatctctgac ctaccatacc ggttccgccc aggataccta cacgtaattt ttcactcatt
     2161 tttcatttcc tcctcatctt tgtgcatttt tattttatct gcaaaagacc tgttcagttt
     2221 ccacttactg aatgcggtct tccaaagcct gttgatatac ttctatggca tttgttttca
     2281 ggtattcttt atttttctca atgacctgct tcatcatttc cgcaccggga gcaccgatgg
     2341 tcagccgctt ttccacacag gtctttaagg aaatggcatc gtaaatgtcc tcatcaaact
     2401 tatcactgat ggaacgtaat tcttccaggc tcagggcatc aatggaagta tccttttcaa
     2461 tacaatacag taccaggcgt ccgataatgc cgtgagcatc ccggaaaggt acgcccttac
     2521 ctaccagata atctgcggca tccgtagcat tggtaaatcc gttcatggca ctctttgcca
     2581 tacggtcctt acggaacttc atggtcttta tcatgccggt aaataaggca atacagtccg
     2641 ctgcggtatc catggcatca aaagccatct ccttgtcttc ctgcatgtct ttattataag
     2701 caaggggcag tcccttcatg gtggtaagta acgccatcag ggcaccgtag actcttccgg
     2761 tcttgcctct taccagttcg gcgatatccg ggttcttctt ctgaggcata atgctgcttc
     2821 cagtggaata ggcatcgtcc agttccacga actggtactc attggaattc cagataatga
     2881 tttcttcgct gaaacggctt aaatgcatca tgatcgtgga aagtgcggaa aggaactcga
     2941 tcagataatc tctgtccgcc acggaatcga tactgtttaa tgtcggtcct tcaaaacgaa
     3001 gcagctttgc ggtatactca cggtctaacg gatacgtggt accggccagt gctcctgctc
     3061 ccagaggaca ataattcata cgtttgtaaa tatcggccag acgctgtctg tccctcttga
     3121 acatttcaaa ataggcacca taatgatgag ccagagtaat gggctgtgcc ttctgtaaat
     3181 gggtgaatcc tggcatatag gtctctaagt tgttctccat ggtgtccaga atggcttcca
     3241 gaagcttttc cagaagtccg tctgtctctg ccactctcgc tctggtatag agacgcatgt
     3301 ccagcgccac ctggtcgtta cggctgcgtc cggtgtgaag ctttttgcct gcatcaccga
     3361 tccggtcgat cagggttgcc tctacaaaac tgtggatatc ctcctgggtt tcgtcgattg
     3421 taagctttcc ggcatccaca tcctctaaaa taccggtcag tccttcgata atgctctttc
     3481 tctcctcctc ggtgaggatt ccctgctttg ccagcatggt ggcatgtacg atacttccgg
     3541 tgacatcttc atgaaacaat ctcttatcga atgttatgga cgcattgaaa tcaaatacca
     3601 gctgatcggt ctgtttcgta aagcgtcctc cccataattg tgccatggtc attctcccat
     3661 ctttttattc tgtcagcagc ctatcagaca atttttctca tagacattga ttgcaattaa
     3721 tcatatcaca aacgacactt taatgcaata cagtgaattt catcactttc gggtagcaaa
     3781 aacacttttt tcttttaaat tcatactata acaataaata ttcaagttaa ggaagtatgc
     3841 ctttttatgg gagctctcat cgaattaaaa aacataggtt actcctatca taatctggcg
     3901 ggcgagaccg gtgccataaa agatgtctcc tttcaggtgg aggaagggga atttgtcgct
     3961 ttagtaggtc ccagcggctg cgggaagtcc acgctgctgt ctatcattgc gggactgctt
     4021 gctccggaga ccggcaccat cgtagtcaac aatcccgacg gatcccttca ctatccccgt
     4081 gtcggctata tgctgcagca cgatcatctc ttcgaatggc gtaccatcta ccagaatgtc
     4141 actctgggac tggagatcca tcatgctctt accaaagagc gtaccgacta tgtgaaacag
     4201 cttctgtccg actatggtct ggaaagcttt caggataaac gtcccagcga attgtccggc
     4261 ggcatgaagc agcgtgctgc cctgatccgc acccttgccc tggatcccca gctgctcctt
     4321 ctggatgaac ccttcagcgc cctggattac cagaccaggc tcatggtatc cgcggatatc
     4381 tgccggctga tccggcaggc tggtaagact atgatcatcg tcactcacga tctgtcggag
     4441 gccatcagtc tggccgacaa ggtctatgta ctgtctgcga ggccggcgac gatcaaggcc
     4501 gttctcccca tccggctcac gcttccggat gcatcaccgc tggctgccag aaatgcgcca
     4561 gaatttccat attatttcaa acaactctgg gaggaattaa cggatgagac cacaaattaa
     4621 acaacctctg accgttcagg aacaatatct tcgaaaacac aaaagccatc acagacaggt
     4681 ctccttcctg cgcttttttc ttctgggaat ctttttactc ctgtgggaag taagtgcaga
     4741 tcttgactgg atcgacagtt tctttttctc cagtccccac agagtcattc tgtgccttgg
     4801 agaattatgg acagagcggt cccttccgat gcatatcggt atcacactgt tagaaacgat
     4861 cctgagtttc ctgctggtat ttttcgtcag tcttctgtta tccactctgc tgtggttttc
     4921 caggcagctg tcggagatct tagagcctta tctggtactg ctcaacagcc ttccgaaatc
     4981 cgctctggct cctctgttta ttgtctggct gggcaccggg atcaacacca tcatcattgc
     5041 tggaatctct gtggcagtat tcggatccat catcagcttt tacaccgcct ttcatcaggt
     5101 agatccggaa aaggagattc tgatccgcac actgggtgga aaccgcaggg atatcttttt
     5161 caaagtggta ctgccgggat ccattcccac cctgctcagt accaccaaag tcaatatcgg
     5221 gctgtccctg gtaggtgtta tcatcgggga atttctcgcc gccagacggg gactgggcta
     5281 cctgatcatc tacggttccc aggtcttcca gctggatatg gtaatcagtt ccatcttact
     5341 gctatgtgtg attgccatgc tgctctacct gctgacacag tctctggaac attacagcaa
     5401 aaagcaccgc aactgacctt tcggagatcc gcaggcgttt tcgtcttacg gtctcctaca
     5461 taatttacgg ctttgtgaaa cgttctgcga aaataataaa ttttacagtt gcaaaatttc
     5521 ccaagaacat atataataac ctagtccgct gaaatagctc agttggtaga gcacttcact
     5581 cgtaatgaag gggtcgtggg ttcgagtccc attttcagct tat
//
LOCUS       DAWDHD010000168         5602 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_838_length_5602_cov_10.656751, whole genome shotgun sequence.
ACCESSION   DAWDHD010000168 DAWDHD010000000
VERSION     DAWDHD010000168.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5602)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5602)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5602
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_838_length_5602_cov_10.656751"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1140 a         1660 c         1231 g         1571 t
ORIGIN      
        1 ttctctatgg tccggacaca aaaccctaaa tgccgagcca atttctctaa aagttctgca
       61 ttgcctgctc catttttccc aaaagacaga tcttcacctg ctgccacaaa acgggtattc
      121 atctgcttcg ccagaatctc tgtcacgaaa cgctccggtt ctgtggctgc agtctctttt
      181 gtcagcggga actcgatcag gatgtcgatc cccagatgtt cgaagagacg ccttttttct
      241 tctcttgtcg tcagttcttt tccatccgac agtccgaaca gcaccgccgg tgtgggatca
      301 aaagtgaaaa cgcaggctgc cagtccgttc tttttcttat tcaagatttc ctctaaaagt
      361 cttctgtgcc ccaaatgcac accgtcaaat ttgccgatgg ctactgctgt atctttttcc
      421 agataaaagt ctgttgttcc tgcaatgatt tccaagtcta tttcctgctt tctgtattac
      481 ttgggaagat cttcacgggc ttgtaccatt ccttcgtttc atcatacgca taaatgccca
      541 cgaagctgtc atcctctctg tagatgcgca cccactctcc tgccggatgt gtggtctgtt
      601 ccacagtctg tcccggataa aaagcattcc cgttatcgat cagtttccgc cacttttcct
      661 ccacatgcaa catgggaaaa tccgaaaaaa tacggtctgt gggaagcagt gcttcctcca
      721 gcctctccgt gtcccgaagt tcctgcagct gtgcaagtgt cagggcatct gccagccgga
      781 attccccgac tctggtgcga acgaggctct gcatggtgcc gccgcagcac agtttttccc
      841 caatatcggc acagagactg cggatatagg tccccttgga acattccacg cggaatttta
      901 ccacgggaaa ctttatttcc agaatctcaa tcgccggcag atccacatgt cttgcctgcc
      961 gttccacttc tttgcccgct ctggccaact catagagctt tttcccattg accttcagtg
     1021 cagaatacat gggtgggatc tggtcatagc cgccgacaaa accggcgatc gcttcacgta
     1081 cctgtgcttc actcaccgcc acttcccgct cttccagaac ggttcccgtg atatcctggg
     1141 tatctgtgat ctgtcccagc agaagttctg ccacatattc cttggtcctg tccgtcagca
     1201 tttcacatgc tctggtagca ttccccagac atacaggaag cacaccggta gcctgtggat
     1261 ccagtgtacc tgtgtgccct atctttttct gccggcagat cccgcgaagc ttggctacca
     1321 catcatggga ggtaaagccc gcttccttat atacattgat gatgccgtcc attaaccctg
     1381 ttctccccgc agttgtttgt cgatgtgcag tgacaaattg ttgacacaat catggaaggt
     1441 tcccttcata gtgcagcctg cggcacgtac atgtccgccg cctccgaaga aagatgccac
     1501 cttcgccaca tctactttct cattggaacg catgctgacc ttgtactcca gtacgttggt
     1561 ctcatacatg aaaatcgcac agtccacgcc cttgatattg cgcaactggt tcacaatgcc
     1621 gtccagatct cccggtaccg cattgtagaa ttccatggtc ttgcggtcca ccatgctcac
     1681 gatgcagcgt ccgtccatga aacgaatgct ctccatcaga gctctgccca tgatctgggt
     1741 ctgcagatag gtcttctggt aaaagctttc ctcgatcagc ttcggaaaat tgaatccgaa
     1801 ggagatcaga aacgctgctg cctgcaacgt ctccggtgtc gtattggaat actggaatac
     1861 accggtatca tggatgatgc cggtatacag cgcttccgca atatccttgt caataagttc
     1921 cgcatccata atgtgataca gcacctcaca ggcacttccc acttccggac ggaccagatt
     1981 cacatcaccg caaccggaat tgctgatgtg atgatccatg ttgatcttct ttttcgcatt
     2041 ctggaaatac gtctccgcat ctcccagacg atccccactg caatccagcg caaaatacac
     2101 atcaaaaggc tcctgcacgg gacattcggt ctgaatctca tcgtatccct tcagatagga
     2161 aaactcttcg gacggcttct gcagatatac ggtaacttcc gcttcgggaa ggagtttacg
     2221 cagatacatc tgtaaggaaa gacatacacc cacacagtct ccatccggac gaacatgtcc
     2281 gctgatgccg atctttttcg cacccagaca ttcttcacgc aaatccatta ctcgtcacct
     2341 tcctctgttt cttccgtatc ttccaccact tcattttccg gcttctgtgc ctgttcatcc
     2401 gctgcaatga cctcatcgat cttgtgggac atattgacac cgtattcaat ggactgatcc
     2461 atcacaaaag tgatatccgg agtattacgc agattgatgg tacgagccag ttctctgcgg
     2521 ataaagcctt cggcgctctt cagtccctgc agggtagact ttctgacttc ctcgtctccc
     2581 agcacgctga tccatgcctt acagctcttc agatccggtg ccacttccac agcgaccaca
     2641 ctggtccagg gactgatccg ggggtccttg agaccgcccc ggatgatctc tgccagaacg
     2701 cgctgtactt ccccgttgat acgggtattt ttcacactat tctttctcat tcttattcct
     2761 tcttaattct ttcctgcggg tatcgtagac gtcttggcga gcatccgcga acagaatgcg
     2821 tttcgcgtaa ccccgttgaa aaacgtcggc acttgaatct tgcaagcaaa gcgcaggaag
     2881 attcttagtg tccgctacgt tttttgcaga gcgcgagcgt gggtaagcgg aatgcatctg
     2941 cgaacggatg cgttctagta acctaccgtg gtacttccac catgatatat gcttctacga
     3001 tatccagctc ctgcatcttg tcaaagcctt cgaatacgag accacactca tatccggcct
     3061 taacctcctt cacatcatcc ttgaatctct tcaaggaagc caggttgcct tcgaagatct
     3121 gctcgccctc acgggtgata cgaaccttac agcctctctg gaacacaccg tccagaacat
     3181 aggaacctgc gatgttaccg atagcggatg ccttgaagat ctgacgaacc tctgcatgac
     3241 cgataacctt ctcctcgtat acgggatcca gcataccctt catggcagct tccacatctt
     3301 cgatagcctg gtagatgacc ttgtacagac gtacatccac gccctctctc tctgcggttg
     3361 ccttggcagt cgcatcggga cgaacgttga atccgatgat gatcgcgttg gacgcacttg
     3421 ctaaagatac gtcggactca ttgatggcac ctacgccgcc gtggatacat ttaactacta
     3481 cttcttcgtt ggacagcttc atcagggact gttttacagc ctcgacacta ccctgtacgt
     3541 cagccttgac gatcaggttc agttccttca gattaccctc ctggatctgg ctgaacagat
     3601 catccaggga cattctgccc ttggtctctt ccagcatctt ttccttgttc tgtgccagat
     3661 aggtctccgc ataggatttt gctgtcttat cattatcatg tgccaggaat acttcacctg
     3721 cactgggaac atcggacagg cccaggattt ctacaggggt agacggtccc gcttctttta
     3781 ctctgcgtcc cttatcatcg atcatggctc ttaccttacc gtgtgccacg cctgcggaaa
     3841 tgaaatctcc cacatgcagg gtacctttct gtaccagtac ggtagccacg ggaccgcgtc
     3901 ccttatccag ctctgcctcg atgaccagac ctcttgcttt tctcttgggg ttggccttga
     3961 gttccatgat atctgcggtc agcaggatca tgtcaagcag ctggtcgata ccttctccgc
     4021 tcttcgcaga taccggtgca aatacagtac ttccgcccca atcctcgggg atcagctcgt
     4081 actctgccag ctcctgtttt acacggtcaa tgttggctga gggcttatcg atcttgttca
     4141 ctgctacgat gatctccaca cctgctgcct ttgcatggtt gattgcctct acggtctggg
     4201 gcattacgcc gtcgtccgct gctactacca ggatagcgat atcggtcgca ttggcaccac
     4261 gcatacgcat ggcggtaaat gcttcgtgtc cgggagtatc taagaaggtg atcttacggt
     4321 ctcccacatt tacagtataa gcaccgatgt gctgggtgat accacctgcc tcacgatcgg
     4381 ttacgttggt cttacggata gcgtctaaca gggaagtctt accatggtca acgtgaccca
     4441 taacacagat tacgggaggt ctctccacca gatctgcctc gtcctcttca tcttccttca
     4501 acagctcttc gatgacatct accttgacct ctttttcaca gagaatgtca tattccatgg
     4561 caatgttctc tgcatcttca taggagatct cctggttcac ggttacaatc ttaccttcca
     4621 ggaacagctt cttgatgatg gctgcgggct gtaacttcat cttctcagcc agttctttga
     4681 tggtaatgga ttccggcagc acgatcacct tgatctgctc ttcctgtacc tcttccttct
     4741 tcttttccgg cttgatgaaa cgtccaggct tgttcttcag cgcgtcctcg tcctcgtaga
     4801 tatggtcctt cttggaacgc ttgttgtcct tctcctggct gaaacgtctc ttttcatctt
     4861 cgcgcttctt ctcaagatcc ttgcctgcag tttccggagc gaagccctta ttctgtctgt
     4921 tatctccgaa tcgattaccc gatccgccat tgcctcttcc gtcgcggccg ttattgccgt
     4981 aaccgcgctc accattgccg ttaccgcggc gatcaccctg accgccttcc atacccggac
     5041 gaccctgtct gttaaatcct cctccctgag gtctgccatt gcccatacct cctctgttac
     5101 cattctgtcc gccctgacgg ttaccctgtc cgtagccacc ctgacgattg tcacgattgc
     5161 cgtctctgtt gtcacggttg taaccgccct ggccacgctc gttctgtcca cggttgtcac
     5221 gattattctg accgtaaccg ccctgacggt tatcgcggtt gtcacggtta ccgttctgag
     5281 gtctgtctgc cggtctggtc tgtgccacat tctgtgcagg ctgtgctgcc tgaggtgcct
     5341 gtgcggctgc agtattctgt actgcctgct cctttaccgg tgcctgtgtc tgaggctttt
     5401 tcgcaggctg ctgtccacgg ttgccgggta ccattgccac gcttggagtg ggagacggag
     5461 gggtcagcgg cttgatggga cgtacgggag cctgtgcctg aacacggttg ccgtaggaat
     5521 tattctggta accattgttc tggtagctgc cgccctgatt cggacgctgt ccgcctgcat
     5581 ttctctggcc ctgtccactc tg
//
LOCUS       DAWDHD010000169         5532 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_847_length_5532_cov_9.396750, whole genome shotgun sequence.
ACCESSION   DAWDHD010000169 DAWDHD010000000
VERSION     DAWDHD010000169.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5532)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5532)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5532
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_847_length_5532_cov_9.396750"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1342 a         1456 c         1089 g         1645 t
ORIGIN      
        1 cgtggaaagc ttgtacaccg gatctcccac agccatcagt tcattaccga gcatctgttt
       61 cttctcgtaa tttttgttat caaatacttc ctgggtaaca ccttccacgg tcagattttc
      121 ataaccgtcc gtccagtaag ccacgatacc ggtggtgggc gcataacaga aatttacaat
      181 atttgcagcc gaaccgttat ttccgttttc aatgctttgc agcatattta cattggccag
      241 tttcagcacc gtgttcttca aagaatattt aaaatcatag gtactctcgt aatttttgtc
      301 atcataccca tgcataaaat tcacgatttc attacggaat tccgaaagtt cctggtcact
      361 cagggtattt tctccaaggc tggcatcctc cagttcttcc gtcagccggc ccgtctcgtc
      421 cacgatgtaa acagtgtctc ccttggccac ccgttcgcct tctcttgcat aaaaattcac
      481 atatccggca gtatccgtac ttacaacttc ctctgtgcgg attgacactc cacggtaaat
      541 attattggta gccagtgatc cttccgttac ttcataacgc acgatgtggt ctgtctgaaa
      601 atacagtatc acacaaatga ttacataaat aaaaatagct ccgaagatga gcataccgat
      661 attgaggttc aaaggttttc tgtatttttt tatttttctc ggttgttctg ccattggaaa
      721 acctcctttc ctctccctta cgtttgagaa gccccggttg gcatccgagg cttctcatac
      781 gtgaaaatta taattcgttc ttcttacaaa gatacaatga tcttatcctt cacagactcc
      841 ggtaattcgg attcgtccct ggatacactg aggataaaat ccacctggaa gaaatcactc
      901 attctctcca gtttttcaac aactgcggaa atgtctttgt cttccagtgc tgcgattttc
      961 agaaagctgt caaaatacat ctgctccaga tcatgatcct gagaaatgat accgcatacg
     1021 aatcccagaa attcgctttc attctcgatc atataacggg aaacgtcgat cagacgcacc
     1081 ttgttgttca gttcatacat atgtttcgta cttttgtcca gatatacgat acttcccgca
     1141 atatccttta cctcgctgtt gactttgtcc aacagctgtt ttgtcttgcc cttgcccttt
     1201 ttgcctacaa tcaactgaac cattggtgta accctcctgt cgtaaaattt ataggaactt
     1261 cacgcaacct atttacttta ttatagttta taacccctca aaaaacaaca gttatttatt
     1321 gatttgctcc aggagttgta ccatttccgg gtacagcaca tcactggctg aggaattcag
     1381 gattaccgat atataaggag ctccgcttgt gtctctggat aaaaggatca gacaatgtcc
     1441 cgccgcgttg gtcgtacctg tcttgccgcc gatgactgta atgttctccg gtgcctgaaa
     1501 atctccccgc aaaaaacggt ttgtgctgtt caccgtaagt tccttggctt ttccgtcctt
     1561 gtcataatat gcggtttggt agcttgtcat gtgaatgatc tcattaaagg aatcatattt
     1621 gattgcttca ttaaagatca gatacaggtc ataggctgaa gtataatgat tgtcatccga
     1681 cagaccatga ggattggcaa aactggtatt tgtagccccc agtctctgtg cttcctcatt
     1741 catcatctca acaaaatgat ctacactgcc gccgatattc tccgcgatca gcattgctgc
     1801 gtcgttggcg gaatacatca gcagaatatg taatgcctga tccaatgtca tggtatctcc
     1861 ggcttttaat ccgcacagct gtgcaccgga ttccgtgatc tttacgctat ccgtagctgt
     1921 cagcacagta tccggtgacg cattctgtaa tgccaccagt gccgtcatta ccttggtcag
     1981 gctggccgga tacaatcttt catgtgcatt tttggaatat aatacctttt tattgtttac
     2041 gtcgaacagt acagcggccg aagcctgtga catgtctacg ctgccatccc ccgtctggtt
     2101 ctccgtcacc acacacagat ccgcagcaaa gggctctgcc ctgcccgctc cggttcctgc
     2161 cagcacattg aaactgctga cactggaatt gacatcgtac ggaaatgtat acgccacatt
     2221 accgcatccg gtaagcagac tcatgcagag gatcaaggac atgcagaagc agatccgacg
     2281 aactttattt gtacatttca cgccactcta aatctcctct gtctaaggac ttgatcaaca
     2341 gttccgcgct ggcgagattc gttgccaacg gaatattatg catatcacac aagcgcatta
     2401 cattgcttac atccggctca tgggccttgg gtgccagtgg atcccgcaaa aagatcacca
     2461 gatcgatctc gttgtgttcg atctgtgcac cgatctgctg ttctcctccc agatgtccag
     2521 caagatattt gtgtatattc aggttggtga cctcttcgat cagcctgccg gtagttcctg
     2581 tggcatataa ttcattttta cttaaaattc cgcgatatgc aatgcagaaa ttctgcatca
     2641 gtttcttttt ggcatcatgt gcgatcagtg caatgttcat atagtccata ccccttctct
     2701 gtgtaatgta tgatttcttt tgtctagctt ctcttcgcct gtaatcgtac gttcagtaca
     2761 acacccattc caatatacag actcatcagc gaggtcaggc catagcttac aaaaggcaac
     2821 ggaagtcctg tattcggtaa aataaacgta gccactccga tgttcacaaa gctctggaaa
     2881 gcgatcagtc cccccattga cgccgctatg atcgtccccg cggtatcttt cgcctttccc
     2941 gcgatagaaa cgcactccat tgccatcagc atcagcagta tgatcactac gatacttccc
     3001 ttaaaaccaa actcttcacc gataacagcg aatatgaaat cggtttccgc ttcagacagg
     3061 aaattgccat tctttaccga tgtgatctca ttgtttttat agcccttgcc gtccagttct
     3121 ccagagccta tggccattac agagttcaac tgctggtaag ccagatccgt ggaatattcc
     3181 tccggattga cgaaggcaag gatacgattt ttctggtaag tctccaatat ggtactgtcc
     3241 ggctgcattg ccagagatac cagtatgatc actgcaggaa ttgccaccgc caatgctccg
     3301 aagatcagct tataactgat gcctccggca tacaggatca cgcagaaaat aaagatcagc
     3361 acaaggctgg tagacagcgc cggctgttcc tttaccatga tccagggaac acccataagt
     3421 accgcaatcg atgcgatcac agggaaagta ttcagcttct ccttatattt cataataaac
     3481 tgtgcaaaga ataaaatcaa taaaattttt gcaagttctg aaggttgaaa ttgcagtcca
     3541 ccgatcttaa gccacctctg ggctccgttg ccttcttcac ccatcaggat taccagtccg
     3601 agcagtacca gattgcctat gtacatcagc cagtaaagct tcaggatcac ggaataatta
     3661 aaaaaagaaa gggcgatcat gataacaagc cccgcaacta cacccgccat ctgtttggtc
     3721 tgctgggcag gttccgcact gccgatggtt atgatgccga tgatggacag tgcaagtatc
     3781 agtatgatca gtttaaagtc ataatcccgt aatctataat tcttaaacat ccgtttgacc
     3841 tttctgttct ttatgctcct tatggcattt tcgcagtctt ctgtaaatca ataatgggaa
     3901 tattggcaat caggctgggg ctcttattcc gtccgccctt actgcccttt gcctgtatct
     3961 cgatttccat atgatccgta tcgatccgca tatatttcga aatcactttt gtaatatccg
     4021 ccttgatcag ttccatcatc tccggagaac agttcacccg gtccgatatc agcaggattt
     4081 tcagacggtc ctttgccacc tggcaggagc ttttcctgcg aaaaaaatgt ttcatattca
     4141 tcccgtcccc ttcctatgct ttccgaaaaa taccggttac ttttgtccaa aaagatactc
     4201 ctttttcgaa atccaggaac ggaatttctt ttcccagcac acgatccacc acattgcggt
     4261 aagccttacc cgccggtgta tcgcttccga ctaatggttc tccctgattg gtggcgatga
     4321 ctacattctc atcatctgga acgattccga tgagcggtac cgccagaata tccaccacat
     4381 catccgcaga catcatatct ccgcgcttta ccatatccat gcgcagtctg ttgatgatca
     4441 gatccatctg tttgaagccg ttggcctcca gcagtccgat aatacgatct gcatctctga
     4501 tggccgagac ctccggtgtt gttaccacca gtgccctgtc ggccccggca atagcattct
     4561 ggaagccctg ttcaattccc gccgggcagt ccagaatgat gtaatcaaat tgttctctca
     4621 agtggtcgat caccttcacc atctgtcccg gggtgactgc cgatttatct ctggtctgtg
     4681 ccgacggcat cagatacagt cccggatgtc tcttgtcccg gatcagtgcc tgtttgattc
     4741 tgcagttccc ttcgaccaca tctaccagat tgtaaacgat acggttctcc agtcccatga
     4801 ccacgtcaag gttacgcaga ccgatatccg tatcgatcag gcagactttg ctgcccgctt
     4861 ttgcaagacc tgttccaacg tttgcggatg tagtggtctt acctacaccg ccttttcctg
     4921 aagtgacgac aatgacttcg gagcttttct tctccgtgga aacgttttgt tccattttgg
     4981 attcctcctg tttgattttg tcgtcccctg gattctttta aaaagtaccc agtaattctt
     5041 ttgtaagggg atcaaatacg atcttcccgt tcttcaggtg tgcaattttc ggttgtactt
     5101 tgggacgaat gccccatttc gactgtttcg cattggtttt atatttgaaa tcgccgattt
     5161 ttaatctttc cggctccatt tcaagcgcta caatataaca atcttctctg ccagatccgc
     5221 ccgcataagc ttccccgtac agtcctccca gaatgatgat gttgcctgcg gagatcaccg
     5281 cactgcccgg gtagacatcc cccaggacca caatgctgtt ctcggtttct atgacttccc
     5341 ggtttttgag ggttcccttg tagaactgcc cctcgtcccc ggtggaaagc tttttctcca
     5401 tatgctgcag agctttgata aagttcttgt tcgtggtctc atcatggccc acgatacagg
     5461 cgatcctcac attgctgttc ttccggatgg tatccagaat gcggatttct tccacttcgg
     5521 tcacctcccg tc
//
LOCUS       DAWDHD010000170         5529 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_848_length_5529_cov_10.397881, whole genome shotgun sequence.
ACCESSION   DAWDHD010000170 DAWDHD010000000
VERSION     DAWDHD010000170.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5529)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5529)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5529
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_848_length_5529_cov_10.397881"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1365 a         1423 c         1218 g         1523 t
ORIGIN      
        1 ttatctatgt cacccatgac caggaagagg ctctgaccat gtccgatacc atcgtggtca
       61 tgaaccaggg ctatatccag cagatcggca ctcccgaaag tatctacaac gagccggaga
      121 acgctttcgt tgccgacttt atcggtgaca gtaacattat cggtgcaacc atgatcagag
      181 atcgtctggt agagatcctt ggtgcccgct ttgaatgtgt ggataccggt ttcggagaaa
      241 acaagcccgt cgacgtagtc atccgtcccg aggacgtgga gctggtatca ccggaagaag
      301 gtattatcca gggtacactg acccattcca ttttcaaagg tgtccattac gaaatggaag
      361 ttatggccaa cggtttcgag tggctggtac agtccacgaa aatggttcct gtcgggacca
      421 aagtcggcat caaagtggat ccgttcaaca tccagatcat gaagaagccg gaatccgagg
      481 atgaggaggc gattggagtc aatgaataaa aaattattat cgctcccttt tatcttctgg
      541 tcagccatgt ttgtcatcgt gcccctgtgc atggtcttct actatggtct gaccgataag
      601 agcggtgctt ttacgctgga taatattctg gcgatcgcaa ccgccgagca ttccaaggct
      661 ctgtgggaag cgctgaagtt atccgtgatc agcactgtga tctgcctgct tctggcatat
      721 ccccttgcca tgatcctgtg taacatgaat gtcaaccaga actcgtttct ggtattgatc
      781 tttatcctgc ccatgtggat gaacttcctg cttcgtaccc tggcatggca gacactgctt
      841 gagaagacag gtgtcatcaa cagcatcctc tccaccctgc atctgccggc gttaaatatt
      901 atcaatacac cctctgccat cgtactgggt atggtataca actttttgcc ttttatggta
      961 ctgcctcttt ataatgtact ggtcaagatc gacaagagcg taatcaacgc cgcttatgat
     1021 cttggtgcca acggtgtgca gacctttgta cggatcatct tccccttaag tctgcccggc
     1081 atgttcagcg gtatcactat ggtatttgtc cccgccctga ccactttcgt gatcagtaag
     1141 atcttaggcg gcagcaaaat cctgctcatt ggtaatgtca tcgaacagga atttacccag
     1201 accggcaact ggaatctggg aagcggactt tccatcgtac tgatgctgtt cattatcttc
     1261 aatatggtga tctccgtaat cacggataaa gagggggagg cgaacacatt atgatcaaaa
     1321 caacggcaca gaaaatatat ctggggctga tctttatctt tttgtatgcc cccatcgtta
     1381 ccctgattgt actctccttc aacgccagca agacccgtgc aaaatgggga ggttttacga
     1441 ccaaatggta tgttgccctg ttccagaacg aacagatcat gcaggctctg tggaatacac
     1501 tggcgctggc tgtgttgtct gcactgattg ccaccttcat tggtacgatt gcctgtattg
     1561 ccatgcaatc catgaagcgt accagccgta cgatccttat gggaatcaca aatattccca
     1621 tgctgaacgc agagattgtt accggtatct ccctgatgct tctgttctta agtcttggag
     1681 ccaaatttgg ttttggtacg attctgctgt cacacattac gtttaatatt ccttatgtga
     1741 tcttaagcgt aatgccccgt atgaagcagt tgaaccccag cacgtatgag gccgcccttg
     1801 acctgggtgc ttcccagacc tacgcctttt tcaaggtggt atttccggat atcctgccgg
     1861 gtatcctttc cggtttcctg atggcattta ccatgtcact ggatgatttt atcattaccc
     1921 actttaccaa aggtcccggt gtcgacacac tgtccaccaa aatctacact gaagtaaaga
     1981 agggcatcaa gccggaaatg tatgcattgt ccaccatcat ttttgtgacg gtactggttc
     2041 tgcttttact ggtgaactac atgcccatgg caaagaagaa gaaataattc tttgaaaata
     2101 gattcaggag gaaaagaaat gaaaaaattt attgcactgc ttctgtgcgg tatccttacc
     2161 gcaactgctc tgaccggctg cgggtcctct tccgccggtg gtgaaaacgg tgaagtcatc
     2221 gtctacaact ggggtgaata tatcgatccc gatacgatca ccatgttcga aaaagagacc
     2281 ggaatcaaag tggtctacga tgagttcgag accaacgaga tcatgtatcc caaggtagaa
     2341 gctggttctt ccgcttatga tgtggtatgt ccttccgatt acatggtaca gaaaatgatt
     2401 gaaaacgacc tgatccagga actggattat gacaagatcc ccaatgccaa ggaaaacatc
     2461 ggtgccgagt attatgagca ggctgccgct tacgatcccg gcaacaagta ctgtgttccc
     2521 tactgctggg gaactgtagg aatcatctat aataagactc tggtagatac ccctcccacc
     2581 aaatgggcag atctgtggga tgaaaaatat gctgacaata ttctgatgat ggattctatt
     2641 aaagacagtt ttatggttgc tctgaagaaa aacggcttct cttctaacag tctggacgaa
     2701 tccgaactcc agatagctac cgatgatctc atcgcccaga agcctctggt acaggcgtat
     2761 gttgtagatc aggtacgtga caaaatgatc ggcggcgagg ctgcgatcgg tgtaatgttc
     2821 tccggtgatg ccatttatgt gatcggcgaa aatccggatc tcgattatgt cattcccgag
     2881 gaaggcacca atgtctggat cgatggctgg gtaatcccga agaatgctcc caacaaggga
     2941 aatgcagaga aattcatcga tttcatgtgt cgtccggatg tagcactgaa gaatttcgag
     3001 tacatcacct acggtactcc taataccgcc gccagagagc tgatcgagga tgaagatctg
     3061 aagaattctc ccattgcttt cccggatctg acgcagtata acaatctgga aaccttcctg
     3121 tacctcggag aggacggcga agaactctac aacaaatact ggaaagaagt tatgtccaac
     3181 tagtctttca gacaaaacaa gcccctgaac tgccaagaca gttcaggggc tttcctgtta
     3241 cccatatttt caggaattct gctcacatcc acaggtctcg catcttccgt catttctcct
     3301 ggaaaatgcc ggatcatcca ccggtgtgaa attcgggaaa tccggtctgg gcggtctgcg
     3361 gtctctgtct ccaccgcgtc cctcgtcttc cagacaacgt gcgccgcagg cattcgagct
     3421 gccacaggaa ctactgcttc cgcaggagtt attggaattc ccacaggaat tgttgtttcc
     3481 gcaggaactg cgattgctgt ttcctataca gctacagccg ttgctacaga aatttccacc
     3541 acaaaatagt aacaataaca gaatgataca gttcatagga tcctctgctt accactgttt
     3601 tttactattc tattccgctt tctccagctg gtgccagtcc atacaccgtt tatgctgcac
     3661 cagttcctct ttgataaacc ggaaggtctt ctcctctccc tcctctgcca gcatttccag
     3721 cacatatagc agttttgcct tcgtctccgg atgaatgggt gccctatctg tcccctgata
     3781 atagtaatca agaggagacc tgtccgtgta ttcgcctccc atatagacct tgctggcagc
     3841 gatccgatcc atgatcattt ccgcaatata cttatccggc atcggcaccg gagccattcc
     3901 accgggcagt gcctgcatgc tgtaatcgat ccagtattcg taatgatgtt tattccggcc
     3961 cttatggtgc agccaggcgc tggaataacc gatatcttct cgttccgcat tgttggggct
     4021 gcgtgtcccc tgatagtatt ttgcccctac ccagaactct gtgggactat acttggacat
     4081 atcatgcaac aatccctgtt tatacagacc taccttaaag cagccctttg ccacaaggat
     4141 cttatgatgt gtgatcgttt taaaatgttt ccagatattc atttcgttct atcatctttc
     4201 tccggcaaca tctgttttct tttttaaaga tcttgccgaa acttttttct ccttgtcctg
     4261 tgatgctgcc atcacaacgc cttcatatac tgcaatactt ctgggggcaa tgatccccgt
     4321 ctgctctctg gactgttctt ctttctcctg cactgtcaga gtatcttccg acttctccgt
     4381 tgccagcaca agattccatt ccataccctt cggcagcttt ggaaatgcca gtttctgcgg
     4441 ctcccagtgc atattcatgg caacatagag gaaagaatcc tccccaccct gtggtgtctt
     4501 tgcatacttt ccacagtaca taatgcccac atgccggtta tagctctctg tctggggcct
     4561 ccaggcattc tgtccgtgat aggacagatc cggataacca caggataacg tatccaggat
     4621 ccgaagctct tcctcaggat gcaggatcgg gtgtgcctta cggaaagcga tcatctgttt
     4681 ccagaaattc aataattccg catttttctc cctgtcctgc cagttcagcc atgtgatttt
     4741 attgtcctgg caatagggat tattgttgcc ctgctgggaa ttgccaaact catcccccat
     4801 gaagatcaac ggggtggact gtgtcagcag caacatacaa aatgcattct gtaactgttt
     4861 tttccgaagt gcctgcactt ttttccgtct ggacggtcct tcctcaccac agttccagct
     4921 gcagttataa tcgttaccgt cgcgattgcc ctcgccgttg gcttcgttat gcttatggtc
     4981 ataagaaacc atatccatca gggtaaatcc gtaataatta gacagataat ggatccgtcc
     5041 catattggcg gggatatgac gcatctcata caatacgccc gatagcatat tgtcatctcc
     5101 cttaagaaaa cgacgcatgg tatacagata atcgtcccgg tattccgcca gattccggta
     5161 ttccggcttc actgccggat catagagttg ctccgtatca aaggaataat gccacagttt
     5221 tgtcccgaac aatgccggat ctccagccag catctgagcc ggtactccct cccccatcag
     5281 atgaaaacca tccacatgat aggataatac ccagaatctt aaaatattcc cgatctctgc
     5341 cgcatccatt ccccggggga aatagaactg caggatcact tccatttttc gcttatggaa
     5401 ttcttttacc ataagacgaa actcttctgc cgggtcttcc cctgccgcat aggctgcctt
     5461 aggcgcataa tagaagcctt cccgatagcc ccaatagttc cgcacccgga cgccgggcaa
     5521 tatacctta
//
LOCUS       DAWDHD010000171         5510 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_851_length_5510_cov_9.415399, whole genome shotgun sequence.
ACCESSION   DAWDHD010000171 DAWDHD010000000
VERSION     DAWDHD010000171.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5510)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5510)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5510
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_851_length_5510_cov_9.415399"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1279 a         1530 c         1192 g         1509 t
ORIGIN      
        1 ccaagggcgg tgtcctgttc tcccccatgc agctgcccgg gcttctgagc ggtcgggtct
       61 gcggagcacc tttcggtctg ggctgcggca ttctcggacc tctggtatcc agtctgctta
      121 ccggtatgcc cgttttcgga tatatgcctg tcatgatgat cgagctgggg ctctatggtc
      181 tgatcagcgg tctggtcatg cagtttcttc gcagcggtaa actggttgtt gatctgtatg
      241 cagctctggt cattgccatg ctgggcggcc gtgtgatcac cggtatcctg cgcgcactga
      301 tctttgcagg cggttcctac agctggtctg cctgggctgc cggatatttc gtatcctgcc
      361 tccccggtat cattatccag ctgattctga tccccatcat ctatgttgca ctggaacgcg
      421 ctcatctcct gccggttcgt tacgctgctg tttctgctgc cgcagagacc gaagctgcat
      481 aaatccatac accctcgagc cggccggctc atcgcatcac agcaaaatgg ctcctctgtc
      541 ttacgacagg agagccattt tttgtctgta gatccgtatg aaatagagga tttccgccac
      601 tgcggtgata ctccatgaaa agacatatag cagatacagg gacggtatcg tatggaaata
      661 ggcaaatatg gtgtaaaccc agattacacg gaacacgcag gaacccatga tgacaatgaa
      721 cataggtata atgctcttgc ccatggctct ggccgccgca atggtacagt ccataaatgc
      781 cgaaatacaa tacgaaaatc ccatgatcgt cagacggtac ataccggcat tcataactgc
      841 ctcatccctg gtaaacaggg acaaaaacgc tcttccgaaa aataccagtg acactcccag
      901 gatcagaccg atgccgaagg aatatgccag gctgatgagg taactgttcc gcaccctttt
      961 cttcttgccc gcaccgtaat tctgtcccat aaagctgccg caggcggtat agaatgctgc
     1021 catgacatcg tataccatgg catctgcatt ggtggctgcg gaattgcctg ccaccatggt
     1081 ggcatcaaag gaattcacac ccatctgcac gaagctgttg gcaaactgga aaattacgtt
     1141 ctgcaatccg gagggcattc ccagcttcag gatatccttc gtaagttccg gatccaaccg
     1201 cagctttccc ggatgcaggg cataaatgtc tttgcagcga agcagtgcct gcaggattaa
     1261 tgctgcagat acatactggg aaatgatact ggccaatgcc actcctgcca catccatatg
     1321 acagacgatc acaaaaaaca ggttcagaat aatattcagc actccggaaa aaccaagata
     1381 atacagaggc ttcttcgtat tccccactgc gctgtacacc gcattaccaa agttgtaaat
     1441 ggccagcgcc ggcattccca gataatagat ccgcaggtac aatactgctt tgtccaaaag
     1501 ttcctctctg gtcttcagca ttgtcagcat ccactcggat aagccgactc ccagtaccag
     1561 taacagcact cccatgatca ggctgaccag tgcggcggaa tgaatcgtct tcgacaggct
     1621 ttccctatcc tttgccccgt aatacagtgc cgtcagtaca ttgataccgc cggacagacc
     1681 aatgaggaat ccggtaaaca tggtgaccag tgtggctgtg gaccctaccg ctcccagcga
     1741 cagggaaccg gcgaactgac cgatcaccgc aatatccgcc atattgaaaa gcacttgtaa
     1801 cagattggac agcattaacg gcacgctgaa gatcagtatt ttctttccta agggtccttc
     1861 cgtcagatcc tgcatattct gtttcatttt caatcctatc cttttatctt cttcccatat
     1921 ttgtatctct gttattactc ttcccatcag catccactct gacgaaaaga acggtcaaag
     1981 agttttttgc tccttgaccg ttccttattc tagcatatct tatctttctt ttctaccgga
     2041 aaatcaattt ttttgacaac ttatgctgcc tctttttctg aatttttccc ggcaccgaac
     2101 agcttgcgga tgcagctgta agcaaccata actcccagta ccataagcag tatggctacg
     2161 atcagctgta agccgtctac caggaaggtt gcctgtccgg ctgccacatt tttcaccagg
     2221 gcaactgtct tctggatcaa tgcagtaaag gttacgacca gcatcattcc catagggaag
     2281 tacagtgtcc agccggtacg accggtagtc tttaagaata ctgacagggc gatcaatacc
     2341 agtgctgcca gcagttggtt cgcagaaccg aacagcggcc atacattgtt gtatccgcca
     2401 agagtcagca gataaccgaa aaacaaagtg atcacagttg caaaatactt attggtcaga
     2461 actctctgca cgggagtcat ctttgcggta tctgtggtgt ctcccaggaa cagctcctgg
     2521 aaggacatac gcccgattct tgctacggaa tccagagaag tcagtgccaa tgcagataca
     2581 cacatggtca tgaatacggt tgccacggtt acgggaaccc ccatcttctc caggaatcct
     2641 gcaactccgg aggagaaaat ggagaaagga gttccatcgg gtttcgtacc gttcacagct
     2701 actgctccga ctactaccag tgcaaccaca cccagcaggc tctctacgat catggcacca
     2761 taacctacca tgggcatatc tttttcattg ctgatggtct tggatgaagt accggaggat
     2821 accaggctgt ggaagccgga taccgcaccg caggcgatgg tgacgaacag agtcgggaac
     2881 aggctgctgt tacctacctg aaaaccgtta aatgcattca gctccatatc gggatgagcc
     2941 acaaccacac caagtacggc accgatgatc atacccagca acataaaggt ggtcatgtaa
     3001 tctctgggct gcatcagaag ccacatgggc attacggatg caaggaacag gtatgccatg
     3061 atgatgtaga tccatgcagt cttggaagca tagatgggaa gatgcatacc tacagcgaac
     3121 attactacca acagaccgat ggctacgact gccttgaccc actcgttcat cttgccgacc
     3181 tttttctgga tcagaccgaa gatcactgct acaacgatga acaacatgga aatggatgcc
     3241 gcactgccgt tggcatatgc cacactggca tcttccagtc cggtacctac gaaggtacct
     3301 gccaccatat cggtaaatgc tgcaatgact aacagagtga acagccagca gaacagcatg
     3361 aacaactttc tgccggtctt accaatgtat ttttcaatga tcattcccat ggattttcct
     3421 tcgttcttca cggaggcata cagcgcaccg aaatcctgta cggcaccgaa gaacagaccg
     3481 ccgatcagaa gccacaggag cacgggaacc catccgaata ccgatgcgag gatgggaccc
     3541 gtcacaggac ctgctccggc gatagaggaa aactgatgag aaaatacagt gaatttggag
     3601 gaaggcacga agtcctctcc gtcttcatgg gtaaacgcag gcgtcttcgc cttctcatcg
     3661 attccccact tttttgcaag ccatcttccg tagaagacat agcctgctac cagcactacc
     3721 attgcgatca gaatgattac caaactgttc atttttgttt ccttctttct tgtgtcttgt
     3781 tgttttgtcg tgacagaaag aaaaattctg attgcccgta aaacaaaaaa gctttccgtc
     3841 tccggtgccc ttaagcaccc aaagacgaaa agctctaagc tctccgcgtt accactttgc
     3901 ttgccatgtt ttatcacatg acctcttgtt cccatctcca tgaaatacaa atatctcatt
     3961 tccaacaggc ttctaaataa cggtagaata ccggtgacac ctacttaact gcttcagtgt
     4021 acttctcaca gaggatagtc tgtcatactc acatgccgtc tcacaccaac cggcggctct
     4081 ctgaaatgct tccgtatgca gatcttgttc tgttcatcga atttcacttt ctgttcgata
     4141 gcaaagatac cactctgatt ttataatgtc aatagttttc ttttaatttt ttgttgatct
     4201 attttatcat tttccctttt tatccgcgat cagaggtttg atagcggaat aaatctcgct
     4261 ggtacgtttg ctgtccttga actctttgtt cagggcattg ttgattccct gtttggtctt
     4321 gtactggcgg atgaatttta ccacctgagg gactatggcc tggtcggaaa gcaccttttc
     4381 cactgcctgg gccagttcat ccggttcttc tgctgccttc ttctctgaag ccttcttatc
     4441 aacagttttc tccgtcgttt tcttttctgt catatttgca gtattcttct tccgggtatt
     4501 tccgctcttt ttccttaccg tttttttcac gggttccaca ggcacaggtt ccagtatctc
     4561 aggcaacggt tcttccagga tctgcacgct cacaaatgct ggctctgcaa tctcctctgc
     4621 atccacttcc gccttcggtt cctccgtcat taccgctgtt gcgttctctg tctcctgttc
     4681 tgtgcttccc gttgccaccg gctctgccgc ttccaccgac agagcctgca cgctctcctg
     4741 ttccgtactt tccgttactt ccattgcgtt ttcttcggtc aggagatttt gttccgccat
     4801 tggattcttt ccccacaaga gattacggat aatgcgcacg ggaactccct gtgctttcca
     4861 gtataccatc agactggtga atccattgtc cttggtcacg ataaagtaat ccgggtgtat
     4921 cccttccttc tgctgctcac agatcaggta accgagatac gttgccagct ggaaatccag
     4981 tgcattcttg gtgccaactg ccaccttgtg gtaaatgatc tgtgccttcg tctcattcaa
     5041 cttccgatgc aggtcaaaag tcatgctgtc cgcattgtca ctgtagaaaa tgcagacggt
     5101 atcctccttc gccagcagtt ccactccctc catgcctcgg ctctttacat tttcaaaatc
     5161 aatcagataa taggccatta tttctcctcc ccatttgttg tatcaatttc ttccgcttta
     5221 ccagtctctt ccggtgtccc ctgctccggt tccgcaggtg catccttctg ttccacagtc
     5281 tctacaggct ctgtgttttc taccggtccg gttgtctccg gcgtcccgga ctcctctgca
     5341 cctgcctgcg gcttttcctc cgtcacggct tccggctctt ttgagatgcc tgtcttcgtt
     5401 ctgctctcct ctgtggcttt cttcttcgcc ggaagctttt cgcgaaagct ttctttccat
     5461 ccggtgagct tctcctccct ccggttcttc cgtgcccttc gctttgctgc
//
LOCUS       DAWDHD010000172         5508 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_852_length_5508_cov_12.335962, whole genome shotgun sequence.
ACCESSION   DAWDHD010000172 DAWDHD010000000
VERSION     DAWDHD010000172.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5508)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5508)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5508
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_852_length_5508_cov_12.335962"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1579 a         1229 c         1517 g         1183 t
ORIGIN      
        1 aaaataccga cctccaatcg ttagattttt ggtctaacaa ttaggggtcg gtacagaggg
       61 cggttgcttt tttgatgttt atttttccgt gcagtctgta cgatactgat aagcgttacg
      121 atgatactga tgatcgtaac aacaataccg acaatgccta ccactgtttc cagcatctta
      181 tgtacctttc aatagatttc ccatctgtat cacctccaat aatactggaa gctctcaaaa
      241 tgtcatcatt ttggcgaaag gtgaaccgct taccgttttg gtaacactcc atttttatta
      301 taaaatacaa tgccgggaat agatacttta gattcggaaa ataagtaacg aaatatgcca
      361 tgcggaaatt ggctgttttc ccgaaaatga aatttgtgca aaataaccaa tttgagtttt
      421 taccgatccc ttgagtgttc ttagcctagt gtccttagcc tagtgtcctt agcgtgtcac
      481 gggcagggtc gattcatgac atatttgggt cgattcatgc agggagaatt attttttcat
      541 acactatggt acaatgaact tacagtaaaa agtaacatta tccgaagcag attacatgac
      601 atgaaaggcg aattccggtg cagagacgca gaatggactg gatagaggga aatgatcgtt
      661 gtagtcggac agaatccctg cggaaatgac gtttgctgat taacggaggt gcatttatga
      721 agaaaaggtt attgagtgtc gttctggcat ttactctgtg ctttggcatg atgccggcaa
      781 cggtgctggc tgaagagatg gggagtgctg aaacggtggc gcctgcccct ggcgatcagg
      841 tggcggtaag ggctgtgaat agtacccatg aagaggatgc ggtaggagat actgtaagtg
      901 gggattcggt aatccccggt ggcgtcagtg ctacgtccct gatgagtaat gacggcatta
      961 cggtagtggc agacgatgcg acaggaactg tggtggcgaa gcttggagat caaaattata
     1021 cggatatggc aacagcgctg caggactggc aggagaaggg tgatatgctg gcgttacaag
     1081 gtcagaatgc cagctacaat atttctacag taacatggag cttccaaaac gccccgggaa
     1141 cgctcgatct gaatggtgtg tgtataagcg gtagtatcac attatcgggg tcatcccgat
     1201 accctctaac aatcctggac agcaaagaaa aagtgagctc aaatggcgca cttctaggcc
     1261 tgacggttga gaaaggtgcg aagctgacgc tgacggatgg cggctatatt agtccattga
     1321 ccgtggaaga tggtgcagaa gatggtattc aacttatggg cggcaaggtg gcagaactga
     1381 catgcaaggc ccccgtctac catctgcttc cggaaggtta tgccctgatg aatggcaatc
     1441 ttacggtaga tcctacaacg gtgataaatg agaggacagc cgcattcatg gtgaaggatg
     1501 cccaaattac taaggtcacc ggcgacagaa ccggcagtgt tgcatacggc gagcataaaa
     1561 tcccctttgc catctctgtg acaaccaatg acagcaaaat cgggcgaatg gcatttgagt
     1621 ggtatcttgt gaaggagaat ggcacgacag caccgctggc aatgagcggt gatacatgga
     1681 gtaggaacgg tgcttacagt tatgatgtta cgatcgacgg cagtgccaat gaaggatgga
     1741 aggacttaga gattaataaa ccctacaaca tcatttgtgt gagcattggc aaggagaaag
     1801 acggagccta ccagtggcag acgcctttaa ggggctttca gcttactatc accccggcag
     1861 gcttgaacag taaaaataca aatgtggaga tcacaaacgc aaacaacctt gtgtacaccg
     1921 gcaatccgct gacagcggcg gtcaaagtga cctacaacgg gacagaactg acagagggca
     1981 aggactacac agtcagcagc aacaccggca caaatgcaag aacatatacg ctgaccatca
     2041 caggacaggg caattacgga ggaagcgtag aaaaagtatt taccatagca ccgacagaca
     2101 agctgacggt agagggtacg gcagaagccg gagcggttta cggtacgagg atttcggata
     2161 tcgccatcag cggtttgaca gtcaggtcga ataatactgt agtggatggt tcttggaagt
     2221 tcaccggacg ggagattccg gctgcaggca cgaccacagc gtataccgct gtttttactc
     2281 ctgagagcag taattataag cagatcaagc ggcagatcaa accgaacatc cgcaaagcgg
     2341 ctgcctgtac gctggcagat atcccagtca gtgtgaagta taacgtgacc gcaggcgaga
     2401 catcgattgg taatgccggt atgcccgctg acgcaggcat gctggtcttt gcaaaaggcg
     2461 gtacagcagg taagaccggt tccgtgacga ttacggattg gaacatagat acagacaccg
     2521 gtaaggtgac ctacacactg gaaggtggtg ctgcgggcga tacagtgact ctgcccgtga
     2581 ctgttacatc tgccaattat gagacgacca ccgtcaatgt aaagattacg ctgacggaca
     2641 aagaaacgca ggcggcgctg gccgttaccg gtggaaccac tgtggaatat ggtcagaccc
     2701 tgcagcttgg catcaatggc ggctccggca caggaaatgt gacctatacg gtaaccaatg
     2761 gggatggtca ggcaggcatt gatgccactg gcagactgat tcccacaaaa gtgggaactg
     2821 ttatcgttac ggcgaccaag gccggggacg atgattacaa tgcgatcacc agcggaccgg
     2881 tgaccattac catcacaaaa gcagcttcca ccggcactcc cggatatacc ggcatcacca
     2941 ctgacggcca gaccttaagg gatgcagatc tgagaattag tggaggctcc ctgcatccca
     3001 cagccggacg actggagtgg attgatgatg cgggcaatgt cctgccggat accacagtgg
     3061 tggaagccaa taagacctat ttgtggcgtt atactcctga ggataccaac tatgcgcctc
     3121 tgaccggttc cgttgtgttg tatcctgtat ctaatcgtgg ggataactcc gatgtcccgg
     3181 atactccatc ctatacgccg gcccctgcgg tacccgatac cggtgacagc agttcggatg
     3241 acgatgatga tgacgatacc cctgctgttt ccaacagcag taacagcact accgtagggc
     3301 agccatctgc aggcccggca gccttggact acgccatcaa acgtggtgat acccttggag
     3361 cgatcgcaag aaaatatgga tgcaccgttg ccgagctcgt agcagccaac agcgatctga
     3421 tcaagaatcc gaaccggatc catataggct ggaaactgaa gatcccgcag cacaggacta
     3481 caggtacaac ggcagctccg gcggacaata acaattcaga cgcttatatt atcaagcccg
     3541 gcgataccct ctgggcaatc gccagaagat atggctgcac catcgcagac ataatcaaat
     3601 ggaataacaa gctgatcacc gtaccggatc ggatccgtgt aggctggcag ctgaaaatac
     3661 cagtaaagta ataaatacac cgctcctgcc aacagatttg taatctgatg ccgggagcgg
     3721 ttgcattttc cacataaaaa tgcgaaaata taagttaacg cattgattgc aagaaagtaa
     3781 ggagaaagct atgtatatta tcgcagggtt aggaaatcca ggaaaagaat atgaaaatac
     3841 cagacataat atcgggtttg atgtcataga cagattagca gaggaagaaa atatcgccgt
     3901 catggagagt aagcataagg cgctgatcgg caagggctat gtggccggac agaaggtgat
     3961 cttggcgaag ccccagactt tcatgaatct gagcggtgag agcatccggg agatcgtgga
     4021 ttattacaag gtggatgaca ccgcagaact gatcgtaatc tccgacgata tcagtctgga
     4081 tgtgggacag atccgtatcc gcaaaaaagg cagcgccggt ggccataacg gcctcaagaa
     4141 tatcatcaaa atgttagggc atgatacttt tatccgcgtg cgtatgggcg tgggagagaa
     4201 gccgaagaac ttcgatctgg cagactacgt gctgggacat tttccggcgg aggaacgaaa
     4261 catcatggac gaagcggcga agaccgcagc agaagctatc cgtatgatca tcactgagaa
     4321 tgcggatgca gccatgaacc attttaacag taagaaagcg taacggttgt atagtaagag
     4381 gaaaaagcga tgcaggcact gattgcgcca ctgaaagaac tggcggaata tgatgagatc
     4441 cggaaagtga ccagacaggg aaaaggaacc gtagctctgt ccggctgtgt ggactcccag
     4501 aaacttcata tgatctacgg actcagcgat ggcctgaaat acaaggtcat cgttacttat
     4561 agtgacttaa aggccaaaga gatctacgag gaataccgtt tttatgacag aaatgtcatg
     4621 ctgtatccgg cgaaggacct gatctttttc caggcggata tccacggcaa tcagctggtg
     4681 caggagcgga tcaagaccct gcgcagagtc gtggagggta agccggtcac cattgttacc
     4741 acctatgcgg cactgatgac accgcaggta ttgtgggagg agaaagacat catccgcacc
     4801 gagcggggcg gcagtctgga cgagagcaaa ctggcatcca gactggtggc tatgggatat
     4861 gagaaagcct atcaggtgga aagcccgggg cagttctcca tccgtggcgg cattgtggat
     4921 atctttgatc tgacggagga aaacccctat cgtatcgaac tgtggggaga tgaggtggag
     4981 tccatccgca gcttcgacat cctgagccag cgttccattg agaagctgga aagcatcact
     5041 gtctacccgg ccacagagtt tgtactgtcg gaagagcaga tccgcaaggg catcagtaag
     5101 cttgaaaaag aagcagacaa acaggaaaaa atcttccggg atgcccacca gccggaggaa
     5161 gcccacagga ttgccacaca ggtcagcgaa ttgaaagagc agctgctgga gttccagagt
     5221 aagatcaatc tggaaggcta catccgttat ttttatgaaa atacggtaac catgccggaa
     5281 ctgctgtcag atatggcggg gcgttctctt gtcttctaca tcgatgaacc ggcgagagtg
     5341 aaggaacagg cggatgccat cgaactggaa ttccgggaga gcatggaaca acgtgccaga
     5401 aaaggttatg tgctaccggg acagatgaat attctctaca gcggagagca ggtggcggcc
     5461 acattggaga aaaatgccgc ggtgacactg gctaccatgg agaccaag
//
LOCUS       DAWDHD010000173         5474 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_859_length_5474_cov_9.999262, whole genome shotgun sequence.
ACCESSION   DAWDHD010000173 DAWDHD010000000
VERSION     DAWDHD010000173.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5474)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5474)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5474
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_859_length_5474_cov_9.999262"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1640 a         1029 c         1494 g         1311 t
ORIGIN      
        1 ggaccgaaac ggtgcgcttc gtgaatttta tgctgaccag aggcacactt cgagtttgtc
       61 cgatgccgag ggaacgcgtt ttgaggctta ttacaatatt ctggacacgc agtacaatta
      121 cgatacggca tggaagccgg gaacggaccg gagcagtgat cagacggtcg gaaatatcga
      181 gggtacgacc aaatatacga cagtccgtta tgtgcagaat cggatcaatg cgatcctgcg
      241 gaaaggaacg tcttatacga gcttctgttt cccgattcgc aattctctgt tttatcaggg
      301 tatggataag acaacgaact ctcaggggca gatcacggtg ctgggagatt acaaggaatt
      361 cggagacggt gccggtcctg atatgatcgt ttcgcaggta aatcaaggac agatcactaa
      421 ttatccttat cagatcacta cagaagaaca gcagactctg acagtggcgg caacacatta
      481 tcagtggctg cagccgaaca tggagctgga taaggacgga gacggcaaga atgatattgt
      541 ggtatggtat tgtctgtccg acctgtccga caatgccaag accagaaagt cgcagaccga
      601 gtggaacaac ggatggggca acgacaatgg cgtgaatctg tataatgtca gccctaaaga
      661 tgtggtaaat aattattata tttacagcat gggtaatgtc acgtattccg gtgcgggaca
      721 tacggagccc acgggaatgg cggagaagaa gctgtttgtg aacaccatgg tagcggctta
      781 caatgcagga accaaggcac cttccgtatt tttccgggat gacaacggca acaccattga
      841 ctccgtatat atgatgtatg atgctgccaa caagatgatc ctggatcgaa gcaataagat
      901 tcaggtggat ttcaaggcta ccgattacaa catcctgtct tcagcaacgg agatccgggt
      961 agagttcttc aaggaatcca cggatgcgga tgccatagag atcgcatatg gcaaggacaa
     1021 gctgaaagtg aagcctctga ccgggcttac ggtaatgaat tccgcaggaa cagcagtgga
     1081 tgcacatgtg atcaccggtg gagacaatca ctttacggaa ggtggaaagg actattatgt
     1141 cacaggtacg tcgtattatt ctgtgaccag tgacggtgta tacacgctgg cattccctgc
     1201 atcggagatg ggcctgttct ccggcagcgg caagatgacg cttaacgatc aggatgccgc
     1261 caccatctat atccgcgcta cgaccgttta cgacaacgga tccaagcgaa ccgaaggtgc
     1321 tgttaagagc ctgacggtca gcgcagcgga gctgtttgag ctggattaaa ggatttgcgg
     1381 gcgacgcaga aaagacaaat tgcacaaaga gcagggctgt ggaaaagtac caatttccac
     1441 agctctgttt ttgtatggtt ttttacctgc aattttcttg cagaatcgat atgcatgtgc
     1501 tatagtaatc acatcttaag aaagtctata taggcaatat tctgaaaaag ttttaatgcg
     1561 tagatgcagt cgatggcgga tcgccgcgaa ttcatggatt gacagaacaa tcggacaggg
     1621 aggaaactat tggaaacaaa cgatattgtg acgaaaaatt ttgaaagcta tgcggatgtg
     1681 gcggcggaca taattaatgt tctgttaaat gaagggagaa gaagggttaa agattatgaa
     1741 atgttgtcgg cggctactga gagtctttac ttggataaag aaaatggatt gcgaaatcaa
     1801 ttggaagatg tagcaaagta ccatatggta aatggggtgc ctaaggtatt gtatttattt
     1861 gccaatcaga caaaggtgga taagggcatg gtattgcgca aggccgggta tgcaggtgga
     1921 gaataccgca gacaatatga aaaacaaaat ccgagcctat atcctgtgat agaaatagtg
     1981 ttatactggg gaaagagcag gtggaaaggg ggatgcagtt tgcaccggtt atttgatgaa
     2041 ggaggggcga ctgcagatat tatatggaag tatgtagatg atgtacaact tcatgtttgg
     2101 gaaatgcgtc atttacccag agaaatacga gagctttttc aaagtgatat gagaatcatc
     2161 gtggattatc ttgcagaggg agatggctat cggtcggacc ggaaagtgat tcataaagaa
     2221 gcaacagtta agatgctacg ggtattatcc ggagatacaa atgttgatga taccggagat
     2281 gcattaaaag aaatgggaat caaagaggag gatgagatca gggtgtgtga attatttgat
     2341 cagtatacaa gaaaagggat tgcacaggga atttcgcagg ggatttctca gggagttgcg
     2401 caagggatta ttataatgtg caaggatttt aataccagtt atgaagaaac tttacaaaag
     2461 gtgagactga aactgaatat ctcagagcag gaagctgaga aagaaatgaa actttattgg
     2521 taaatatgga atagaaactt gactttttct atgtactatg ataaatatat actataccta
     2581 ctggaacagt aggaaatata aagggaataa tatagtatac atagacatag gaggagaaaa
     2641 agatggcatt tgcagcagag accatatgtg tccagggaag taatgagaga aaggatccgt
     2701 tcggcgccat cagcatgccc atttatcaga atgcggcgta tgcgcatccg ggactgggga
     2761 agagtacggg atatgattat tcccgctgca gcaaccccac cagagacgaa gtgcagaaga
     2821 cggttgctct gttggaacag ggaacagagg cactggcatt ttctacggga atggcggcaa
     2881 tcactgcaat gatggaaatc ttttctcccg gagatcatct gatcgctacc gatgatctgt
     2941 atggcggaac attaagactg tggaacatga tcagtcacaa gaatggtatt tccgtggatt
     3001 gtgtagatac ttccaacgtg gatacggtga aggcagcgtt gcgcccggag acaaaagcta
     3061 tctatctgga gacaccgtcc aatccgatga tgaaagtagc ggatattcag gcaattgcag
     3121 atcttgcgca tgagaatgga tgcctgttga tcgtggataa cacatttctg tctccttatt
     3181 tccagaagcc gttgacatta ggagctgata tcgtggtaca cagtggaacc aaatatctgg
     3241 aaggacataa tgatactttg tccggttttc tggtggtgaa ggataatgct ctgtatgacc
     3301 agctctataa tatctacaag actacaggag cctgtctgtc tgcgattgac agctggttgt
     3361 tactccgcgg tatcaagaca ctggcagtcc gcatggaaca gcaccagaag aatgctctgg
     3421 ccatcgcaca ctggctggaa cagagaccgg aagtggaaga agtctattat atcggactgg
     3481 attccagacc ggataaggag ctgattgaca gacagtgcag cggatacggc ggaatgattt
     3541 ctttccgatt gaattctgcg gagaaagctg tcaaaattct ggagagtgtg aagctgatcc
     3601 gttttgcgga aagtctgggt ggcgtggaaa gtcttctgac ctatcccatg aaacagaccc
     3661 atgcagatgt acccgtagag gtcagagagg cccttggagt ggacgagaag cttctgagaa
     3721 tgtccgttgg tattgagaat attgatgacc tgctggcaga catggagcag gcctttgcct
     3781 gagtggggtt gactggtata catagtaatt tagccagttc cgcggaaagt ttcacggaag
     3841 tcccacaaca gacgcaacga gtcaggacgt gcagaaggtc ctggtagaat aggtacgatt
     3901 gacagaaaaa tttaacctgc aacttggggc cacaggcaga aagcttgcag gttgtcgaaa
     3961 ggtaagagga ttcgtatggc tacatacaac tttgataaaa ttataaatcg taagggaacg
     4021 aactgcctga agtacgacta tgccgtggag agaggcaagc ctgcggatgt gctgccactg
     4081 tgggtcgcag acatggactt tacagtatcg gaggagatta cgaaaagcct tcacgcggcg
     4141 gtagatcatg gaatctacgg atacacccag cctaaggatg cttattataa tgccataacc
     4201 aattggatgg aaaaaaacca taattggaag acgaagagag agtggatcat gaagaccccc
     4261 ggtgtggtat ttgcactggg tgcagcggta aaagctttca caaagccggg agatgcggtc
     4321 ttgatccaaa atccggtgta ttatcccttt accaatatca ttagggacaa tgaccgtagg
     4381 gtgattgata atacattggt atacgaaaaa agggtaactg aaggaaagtc gcagtacagc
     4441 atagattatg aagacttcga gcgcaagatt gtgcaggaga atataaaact gtttatcctg
     4501 tgcaatcctc acaatccggt gggaagagta tggaacagag aggaactgca gtgtttggga
     4561 gagatctgcc tgcgccatca tgtgatcgta gtcagtgatg aaattcacaa tgattttgta
     4621 tatcccggat ttgagcatac ggtgtttgcc aatgtggatc cccgttttgc ggagttcacc
     4681 gtgacctgca ccgcacccag taagaccttc aatctggcag gcttacagat ttccaatatt
     4741 tttatctcaa atgagacatt gcgggaagca ttccagaagg aaatcgacaa gaccggatac
     4801 gatgagccga atgcgttggg agcggtagcc tgcgaggctg catatcgtgg cggacaggag
     4861 tggctggatc agttgagagc ttatttgctt gagaatctga acttcctgcg agcatatctg
     4921 caggagaaaa ttccacagat tcatctggta gagccggaag gtacttatct ggtatggctg
     4981 gattgcagtg aacttgggat ttccggcaag gaactggatc agtttatcgt agagaaagcg
     5041 ggactctggc tggatggcgg agcaatgttc ggacccagtg gagcagactt ccagcgagtg
     5101 aatattgcct gccccagagc aactctagag cttgcactgg acaagctgaa ggcggcagta
     5161 gacaacctgg gctaggtccg acagatttga tgtgagcaaa gaaaaatatc tgtggacgct
     5221 tgttccgata ggacaaaaat tatagctgta taatattaac agacatatgg tatattttta
     5281 caatcttgtc catatactgt aacatatcag gccattcatg actgatcacg gcagcttatg
     5341 ctacgcttgt gatccctgat gaatggcctt ttcgatagta ttgccataag gaaaggagca
     5401 cacagtatat gccaagagga cagagaaaat ccctggagga aaagatccag gcaaaacagg
     5461 agttgatcaa tgcg
//
LOCUS       DAWDHD010000174         5439 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_867_length_5439_cov_11.507429, whole genome shotgun sequence.
ACCESSION   DAWDHD010000174 DAWDHD010000000
VERSION     DAWDHD010000174.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5439)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5439)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5439
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_867_length_5439_cov_11.507429"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1667 a         1055 c         1510 g         1207 t
ORIGIN      
        1 tatgacaagg ataatgttag cgaaaggcgg taaatcaatg acagcggaga tcatctgtgt
       61 aggtaccgaa ttattactgg ggaacattgt gaatacgaat gcagcttatc tggcggaaaa
      121 gctggcggga gccggactgg actgttacta ccagacagtg gtgggagata atgtggagag
      181 actggcaggt gtgttgaaat gtgccatgga gcgttcggat gtgatcctgt tatctggtgg
      241 actgggaccc acggaggatg atctgaccaa ggagactgtg gcggaagtct gtggaaagaa
      301 tctctcggtg gatgatcaca gcatggagcg cattgccgaa tttttcgcag tcagagatat
      361 tcagcccacg gagaacaact ggaaacaggc catggtgcca gagggagcga aggtgctgga
      421 caatgataat ggaacagcgc cgggcattat tctggagacg gagaagaacc gtattgtgtt
      481 gctgccgggc ccccctgcgg aactggttcc catgttcgaa cagcaggtgc ttccttatct
      541 ggatacactg actcccgggg tgatctgttc tcagatggta aagctttgtg gtgtgccgga
      601 gagccaggtg gaggataccc tgaaggatct gatcgacgga cagaccaatc ctacgatagc
      661 tacctatgcc aagacgggag aagtacatat ccgtgtcacc gcttccgggg aagataccaa
      721 ggcagctgga aaagccatta aacctgtggt gaaggaactg aaatcccgtt tcggagcgga
      781 tatttacagt accaaggcgg agactaccct ggagatggcg gtagcagatc tgctgatggc
      841 caatgatctg acggtgacaa ccgcggaatc ctgtaccggc ggcatgatcg cggcaaggat
      901 catcaatgtc cccggggtat cggagtgcta caaggccgga ctggtgactt attccaataa
      961 ggcaaaacgg aaattcctgg gagtgcgtaa gagcacgctg gacaaatacg gtgccgtcag
     1021 cagcaagact gcctcagaga tggcaaaagg agccgcactg ttaatgaaat cggatgtggc
     1081 tgtggcagtg accggaattg caggaccgga tggcggaacc gaggaaaagc cggtaggact
     1141 ggtatatatc ggatgcagca taaagggaga agttacggtg aaagagtatc atttctccgg
     1201 aacccgcagc cagatcagag aggcaactac ctctgcagca ctgattctta tgcgcagctg
     1261 tattctgaaa tacttcagca aagttacttt tggtaaaaag taacagaaaa atattttgtg
     1321 agataccctc aaaacgaagg ggtaaaaaat tgtagatagt gacgaaaaca caggaaatgt
     1381 atcatgcgtc cttgcgggaa gcggggatgt gtgatacatt ctttttgtat cttttatctc
     1441 tgattatcga cagacatttc aggatgttca ggcattcgcc aaaatttcag acgatcatcg
     1501 gaacgaaaaa ataaggggga atgcttatga gggagcttta atgtacacaa aatatgtata
     1561 ataaaaatag acattgacaa acacgaacaa atgttttatc ctaaataaaa agcagaacat
     1621 ttgttccttg aacaaaaagg agattgacat ggaaaaagac gataagctta aagcattgga
     1681 tgcggcgctg agccagatcg agaagcagta cggaaagggc gcggtaatga agctgggaga
     1741 tcccacggca cagatgaatg tggagaccat ccccaccggt tctttaagtt tggatatagc
     1801 gttaggactg ggcggtattc ccaagggaag aatcattgag atctacggac cggaatccag
     1861 tggtaagacc accgttacct tacatatgat cgcagaagta cagaaaagag gcggtattgc
     1921 cggattcatc gatgcggagc atgctctgga tcctgtctat gcgaagaaca tcggcgtaga
     1981 tattgacaac ctgtacattt cccagccgga taacggtgag caggcactgg agatcacgga
     2041 gaccatggta cgctccggag ccattgacat tatcgtagtg gactccgtag ccgcactggt
     2101 acccaaggcc gagattgacg gtgatatggg agacagccat gtgggtttac aggcacgact
     2161 gatgtcccag gcgctgcgca agctgaccgc tgtgatcagc aagtccaact gtacggttat
     2221 ctttatcaac cagctgcgtg agaaggtagg tgtaatgttc ggtaatcctg agaccactac
     2281 tggcggacgt gcgctgaagt tctattcttc tgtacgactg gatgtcagaa ggatcgaagc
     2341 tctgaagcag ggcggtgaag tgatcggtaa ccgtacccgt gtcaaagtcg ttaagaataa
     2401 gattgcacct cctttcaagg aagcagagtt tgatatcatg ttcggtgaag gtatttccat
     2461 ggtaggagat attctggatc tggcagccag ctgtgacgtt gtggcaaagt ccggtgcctg
     2521 gtatgcctac gagggcaaca agatcggaca aggccgtgag aatgccaagc agtatttaaa
     2581 agacaatccc gccatctgcg atgagattgc cgagaaggtc agagagcatt acggactaaa
     2641 ggcagatgtc actgccgcag cagagaagga agagtagatc catgctggta acacaggtta
     2701 cggaactttc caaatcgaga agcaaagtat atattgatca ggaattcgcc tttgtattgt
     2761 acaaaggcga attgcgtctt taccatataa aggaaggaca gcaattaagc gaggaagact
     2821 atcggactat catgcaggag gtacttccca agcgggcgaa gcttcgtgcc atgaacctgt
     2881 tgcagggaag agaatatacg acggcacagc tgcggacaaa attacagcag ggcttttatc
     2941 cggcggagat tatagagcag gctatcgctt atgtggcggg ctttcattat atcgatgatc
     3001 tgcgatatgc cgtggattat attacttatc acgaggacag ccgcagcagg cggcggatcg
     3061 aacaggatct gcagggcagg ggaatcccgg cagctaccat ggaacaggcc tggcaggtct
     3121 ggcgggagaa gggcggagag caggacgaac agagtatgat ccgtgaactg cttcataaga
     3181 agcattatga tccggaagca gagacagact ggaaggaacg ccagaagatc tacgcatttc
     3241 tggtcagaaa gggattctcc gcagaagcta tccgcaaggc catcggtggc atgcatagcg
     3301 atgatttctg agaataccgg acgacgcata acagcaagaa ataatgcaca aagattgttt
     3361 tgatatagga actatttttg gactaattgc gacttgtact tgacatgggt gtaatttcag
     3421 tttacactaa tactgttgtg aattgcttgt tagaatgtac ataaattgca ttctattatg
     3481 aacgataatt tgtattatcg gagcaaaatg tacaatgaaa atttcataag gaggtgctcc
     3541 tgtagtggac actaatattg taatagtatt agtggcttct gttattgcca ttatagctgc
     3601 agtgctgatc acagcaaagg ttaccacgaa ccgtctgaag aagaacgcgg agggaaccat
     3661 cggcaacgca gaggagaagg caagagagat tattgatgaa gctctgaaga ctgcagagaa
     3721 caaaaagcgc gaatcactcc tggaagtcaa ggaagagtcc attcgtacca agaatgaact
     3781 ggacaaagaa atcaaggaac gcagagcgga agcccagcgg tatgagcgca gagttcagca
     3841 gaaggaagag aacatcgata agaaagcaga tgccatcgag aagcgcgagg caagtttagc
     3901 atccagagaa gaatccctca atcgcatgaa ggaagaagtc tccagactga atgagcagcg
     3961 tgtgcaggaa ctggaacgaa tttcaggatt aacctctgaa caagcaaaag actacttatt
     4021 gaaaattgtt gaagatgaag tgaaacatga atcggccgtg atgatcaagg aaatggagag
     4081 tcgggccaaa gaagaagcag acaaaaaggc aaaggaatat gtggttaacg ctatacagag
     4141 atgtgcagca gaccatgtct ctgagactac aatttccgtt gtacagttac cgaatgacga
     4201 aatgaaggga cgtatcatcg gacgtgaggg acgtaacatc cgtacccttg agaccatgac
     4261 aggtgtagac ctgatcatag atgatacacc ggaagcagta atcctgtccg gatttgatcc
     4321 gattcgtcgt gaggtagcga gaattgcgct ggagaagctg attgtggacg gacgtatcca
     4381 tccggccaga atcgaagaga tggtagagaa ggcgcagaag gaagtcgaag taatgatcaa
     4441 ggaagaggga gaagccgcaa ctctggaggt tggtgtacat ggcattcatc ccgaactggt
     4501 aagattactt ggtaagatga aattccgtac cagctatggt cagaacgcat taaagcattc
     4561 catcgaggtt gcacagttgt caggtcttct ggcagcggag ctgggactgg atgtgagact
     4621 tgccaagaga gcgggactgc tgcatgatat cggtaaggca gtagaccacg agatggaagg
     4681 ttcccatatt cagctgggtg ctgaactgtg tcggaaatat aaagagaacg ctacggttat
     4741 caatgccgtg gaatctcacc atggcgatgt agagcctact tccctgattg catgtattgt
     4801 acaagctgct gatacaatat ccgctgcaag accgggagct agaagggaaa cactggaaac
     4861 ctatactagc agattaagac agttagaaga tatcacaaac aatttcaaag gtgtagataa
     4921 atcttttgct attcaggcag gtcgtgaagt tcgtgttatg gtagtgcccg agcagatttc
     4981 agatgccgat atggtattgt tggcaagaga tatttccaag cagatcgaag ctgaattgga
     5041 atatcccgga cagatcaagg taaatgtgat ccgtgagagc cgcgtaattg attacgccaa
     5101 ataactgacc agtcagaaat gacaatttca tacgaagtaa gaaaagctgt tgtgctgtta
     5161 aagcacagca gcttttttga taaaattttc gaaaaaaggt attgtacaaa gaactttctt
     5221 atagtatgat attcgaagtg tatgattgta tacgattatc caacgtccac agaagaaagc
     5281 gtattcccta tgggaaaaaa cgagaaaggt atggtacgaa atgaaagcat atcaggaatt
     5341 atccaaggaa gaacttttaa cattgaaggc agagctgaac gctgcatatg aagatgcaaa
     5401 aggcaaaggc ctgaaactgg atatgtccag aggaaaacc
//
LOCUS       DAWDHD010000175         5385 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_877_length_5385_cov_9.270919, whole genome shotgun sequence.
ACCESSION   DAWDHD010000175 DAWDHD010000000
VERSION     DAWDHD010000175.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5385)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5385)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5385
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_877_length_5385_cov_9.270919"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1523 a         1123 c         1551 g         1188 t
ORIGIN      
        1 cgatgtggaa ctgttttttg tggaagcggc acttttgatc gagagcgggt atgaacatat
       61 agtggatgaa atgtggtata tctacgccag agaagatgta cgcagaagaa gactttcgga
      121 aagccgcggt tacacaccgg agaagataga aaagatcata gcatcacagc tttcggagga
      181 agaattccgg cagcattgtg attttgtcat tgataacagt gacagtctgg aagacagcta
      241 cagacagatc agacagaaac tggaggcata ttcatggaag gagtaaaaga aaaacaggga
      301 cagctggtat tcggactgga tatcggtacg agaagcatcg taggtacggt aggatactta
      361 aatggcggga agttccatgt gctggcacag cgctccaagg agcatgagac cagagccatg
      421 ctggacggac agatccatga catcggcaag gtgggagaga ctatttccca ggtaaaggaa
      481 cagctggaag cggatctggg cagagaactt acggaagtat gtatcgcagc tgcaggccgt
      541 gtgttgcgta ccgtcactac ctatgtggag cacagctttg agagtgaccg ggagatcacg
      601 caggaagatg tgtattcctt gtgtaccatg ggcgtggaga aggcttacga ggaattccag
      661 aacagcaata cggatacaga tatgaaattc tactgtgtgg gttatacggc catgcggtat
      721 tacatgaatg gctaccagat gggtaatctg gagggacata aggcaaaaag cattgcagtg
      781 gatctgatcg ccacgtttct gccggatgat gtagtggacg gtctgtataa ggcagtagaa
      841 ctggcgggcc tgcatgtggc gaatctgaca ctggagccta ttgctgccat tcaggtagca
      901 attccggaga agttccgtat gttaaatatg gcattggtgg acgtgggtgc aggcaccagt
      961 gatatttcca ttaccaaaga gggaaccatc acagcctatg gcatgatccc cgtagcagga
     1021 gacagcctga cggatattct ggtacagcat tgtctcgtgg aattcgaggt ggccgagcag
     1081 atcaagagaa aatgcagaac ccaggagact atcgaatacg aagatatcat gggactgccc
     1141 cagaccatca aagccagcga agtgctggaa ctgctggatc cggagatcga acgtatgacc
     1201 cagctggtat ccgataccat caaagaactg aacggggaca aaccggtcag tgccgtgttc
     1261 gtggtaggcg gcggtggaat ggtgcccggt tacacggaga agctggcaga aaagctgggc
     1321 atcgtgaagg aacgtgtggc aatccgggga caggaagtca tgcagaccat tacatttgaa
     1381 ctggaaaatg ccagaaagga tgccatgatg gtaacaccta tcggtatctg tttaagctat
     1441 tatgtacaga gcaataattt tattttcgtg gaatttaacg gagagcgggt aaagctctat
     1501 gataacggca aattatccgt gacagatgcc gctatgcaga tgcagttccc caatgaccag
     1561 ctgttcccca gaaaagggga agcccttctg tttactgtca atggtaagac ccgtatggtg
     1621 cggggcgaac agggagaggc agcagtcatc cgggtcaacg gagacgaggc ggatatgtat
     1681 acccaggtac ataatgggga ccgcattgtg gtaacacctt ccacggaggg cgaaccggcg
     1741 gtgctggagc tgggaaaact ctctgaactg ggggatgcac ggcaggtcta tgtgaacggt
     1801 aaacagatca gtctgccgaa gacagcggaa gtcaacggtc acagagagaa tgaattctac
     1861 cggatcggcc agaacgatga tattcggatt cggaattctt atactgtaaa ggaaatcgca
     1921 gaatttctgg atgtgcctct cggtgcggat atcagggtca atgatacagc ggcgcagccg
     1981 gataccagag tttatgagca tttcaccgta agctgggata tgaagaaccc gctgccggaa
     2041 gggagttatg cagacctgcc ggatgcggac acagaagagg atgcctatag agaagagcct
     2101 gtttatggag aaggggctgt gatgcagaag gcagaggctg tggcaggaga agcggcgatg
     2161 acacagggga cagagactgc agcagaaaac gggacggctt ccgtgacaac gcaggggact
     2221 tccgggaagc aggacgaaaa gtacgaaagc gtggaacaga cggcacagag ccaaagcgtg
     2281 tcaccacagg gaccgcatcc gttaacagtg atcgtgaacc attcccccat caccatgcag
     2341 ggtaaggctt cctatgtatt tgtggatgtg tttgattata ttgattttga tctgggatcc
     2401 tccgcatccg caggcagatc cattgtgacg aacttaaatg gacgcccggc acagttcatg
     2461 gaacctttga aagagggaga tgtgatcgag atctactgga aaagcatgca ctagtatgca
     2521 ttaatgccga agatggaaaa cggcaaagag taagacagaa gtaagataga aaagagatta
     2581 agaagttatg agattatgta gtattgccag cggcagcagc ggcaactgta tttatgtggg
     2641 atcagaggcc acgcatctgc tggtggacgt gggaatcagc ggcaaaaagg cggaggccgg
     2701 gctgaacagt ctgggactca ccggaagaga tctggatggc atcctggtga cccacgaaca
     2761 tatggatcat gtggcgggac tgggtgtcat ggcgagaaaa tatgaagtgc cgatctatgc
     2821 cacaacggga accctggaag aaatccagaa gatggggaat ctgggcaaga tcgatccagc
     2881 cctgtttcgg gaagtgaagg aagatgtgaa actgactatc aaggatctga ctgtcaatcc
     2941 catgaaaatc tcccatgatg cggcacagcc ggtgggctat cgtattgctt acggagataa
     3001 aaaggtagga atctgtacgg atttaggagt ctataatgat tacaccgtgg agtgcctgaa
     3061 gggaatggat gccttgctgc tggaagcaaa tcatgatgtg aagatgttgc aggtaggacc
     3121 ttatccctat tatctcaaac agagaattct gggggacaga ggacaccttt ccaatgaaaa
     3181 ttccggcaag ctgctctgtc gaatcctgca tgacaagctg caggctatcg tgctgggaca
     3241 tctcagcaag gagaataatc ttccggagct ggcctatgaa gcggtccgga tggaaatcac
     3301 catgggagag aacccttacc gggcggaaga cttccggatg atggtggcag acaggagcaa
     3361 tgtatctccg gtgatcaata ttgcgtagcg gatgcgcagc gcatcagcca gcattattcg
     3421 ttggagccgc atcatagcac gcttttgcgt catttcatga ctagctttgc tccatagttt
     3481 ggcgctcggt gcctgaaaca tatttgctca caaaatgatg caagtatgat ttctgtgtct
     3541 gaatatgttc ataggctgaa cttgaggttg caaattgtag taaagtgtag tttaatagat
     3601 aaaaataata gcatgatttc atgcagaaaa tgagggcgat tatgaaaaga acaattatta
     3661 cggtagttgg taaggacaca gtaggcatta tcgcaaaggt atgtacctat cttgcggaaa
     3721 acggcatcaa tatcctggat atttcccaga ccatcgtaca gggatatttt aacatgatga
     3781 tgatcgttga caccaatcag atgcagaaaa cttttggtga tatggcagat gaactggctg
     3841 ctctcggaga agagattggc gtagtgatca agtgccagaa ggaagagatc tttgacaaga
     3901 tgcaccgcat ctgataagcc ccacgatttc gaaataaata agacccgaag gggcagatag
     3961 gagccgtaat gatcaattta tatgaagtaa cagagaccaa caaaatgatc gaggaagaga
     4021 acctggacgt gcgtaccatc acactgggta tcagcctgtt agactgtatc gattccgatc
     4081 tggacagatt aaatgagaag atttataaca aaatctacga agcagccaag gatctggtga
     4141 agaccggaga ggagatctcc agagaatttg gcatccccat tgtaaataag agaatttccg
     4201 taactcccat tgcactgatc ggtggtgcgg catgtcatac gatagaagac tttgcaagta
     4261 ttgcaaagac attggataag gtagcacatg aggttggtgt gaacttcctg ggcggttatt
     4321 ccgcattggt cagcaaggga atgaccccgg cggacgaact gctgatccgt tccattcctc
     4381 tggcgctgtc taccacagag cgtgtgtgca gctctgtgaa cctgggctcc accaagaccg
     4441 gtatcaacat ggatgccgta aagctgatgg gtgagatggt cagggagact gcagagtata
     4501 ccaaggaaga tgattccctg ggctgtgcaa agctggtagt attctgtaat gcaccggatg
     4561 ataatccttt catggcaggt gcctttcatg gtgtgaccga agcagacaag atcatcaatg
     4621 tcggtgtcag cggccccggc gttgtaaaga ccgcactgga aaaggtgcgc ggcgagaact
     4681 tcgaggttct ctgtgagact atcaaaaaga ctgcttttaa ggtaacccgt gtaggtcagc
     4741 tggtagcaca ggaggcatcc cggatgctaa atgttccctt tggcatcgtg gatctgtctc
     4801 tggcaccgac cccggccatc ggcgacagcg tagcggatat cctgtgtgag atcggtctgg
     4861 aacatgccgg agctcccgga accactgcag ccctggcgtt gttgaatgat caggtgaaaa
     4921 agggcggtgt tatggcatct tcctacgtcg gcggtttaag tggtgcattc attcctgtca
     4981 gtgaagatca gggaatgatc gatgccgtga atgccggagc cctcaccatc gagaagttgg
     5041 aagccatgac ttgtgtctgc tccgtaggtc tggatatgat cgccattccc ggagacacca
     5101 aggcaaccac catttccggt atcattgcgg atgaaatggc catcggtatg gtgaaccaga
     5161 agaccaccgc agtcagaatc atccccgtaa tcggaaaagg cgtgggagag accgtagagt
     5221 tcggtggcct gttaggttac gcgcccatca tgcccgtgaa taccttctcc tgtgatgctt
     5281 tcgtcaaccg cggcggccgc atccccgcac ctattcatag ctttaagaac taactggaaa
     5341 gttagttaaa tacattggca aaatccacga actcccggag catgc
//
LOCUS       DAWDHD010000176         5340 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_882_length_5340_cov_8.868117, whole genome shotgun sequence.
ACCESSION   DAWDHD010000176 DAWDHD010000000
VERSION     DAWDHD010000176.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5340)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5340)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5340
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_882_length_5340_cov_8.868117"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1374 a         1473 c         1074 g         1419 t
ORIGIN      
        1 gtagcagcca ttaaactgct acgcttaaaa agtctaactt ttgggggtca catcatttcc
       61 cgggtgtttt ttcatccata caaaggctgc cacacagatc accacagaga tcatggagcc
      121 tgtcaggaac tgcatcagga atgctcctca gattttttcc gtggcacgct cttcctctgg
      181 ataagtcagt ccccagtctt ttcggatccg ggtcatcatt ttcataacat cctcggtaag
      241 ctcaagcttc attaattccg gatgtccggc gatggcctgt tccatatcct ccacctcgta
      301 tcccagtgcg tctgccgtag ctcccgccac aatttcctcg gtgtgtccgt cttccgtgta
      361 cgtgatcact gctttctgcc ctctgggata ctcatacatt tccacgtaag ccttgtcaaa
      421 agagatcatg ccccgtttgg gctgcttggc atgcagggac aatgtgaccg ttgccatctc
      481 ctgctcccgg ttcatcagaa gtattcccgc ctgctcgtcc acaccggtag gtgcaagctt
      541 cacctgcgaa gtaatctgat ccgggcagga atttaagaac attctgacaa aggaaagggc
      601 atacacaccg atgtccagca atgctcctcc ggccagattc cggttgaaga accggttctt
      661 catatcgtat tccttataac ttccgaaatt catctgtatc acacgcaatt ctccgaattt
      721 tccggcatcc atgcgttttt tcagttcttt ataaatcggc atatgataga tagtcatagc
      781 ttccgccagc accacaccat gctccttcgc cagctgcact gcctcttcca gttcttcgga
      841 attcagcgta atggattttt cgcacagcac atgcttgcca gctgccagcg ctttccgcag
      901 aaagtgaatg tgagtgttat ggggtgtgga aatataaatg atatccaccg caggatccgc
      961 acatacatcc tgaaagctcg taaacacctt ctgcacaccg tatttttccg caaaggccac
     1021 tgccttctcc ggcgtccggt tcacgacacc atacagtttc tgtcctcttt tttccatagc
     1081 ctgcgccagc tcatttgcaa tgacaccgca gcctgcactt acccagtttg ccatcctatt
     1141 ttctgcattc ctttccacgc gtcttattct catacatctt tgtatccgct tcattgatga
     1201 tcttacggac atccacacca cagccttctg cccagcccac agcactggag accggcatct
     1261 cgccctgact gtttttctcc tgcaatgcag cactgacaga ttcgatcatg gactggatct
     1321 ccgcttccac cggattctcg atcagcatga caaattcatc tccgcctatg cgatagacct
     1381 tacgtctgtc cgtggataat tcatataata cggaagacaa cgtctggatc agcacatctc
     1441 cggcggcatg gccatatttg tcattgacac atttcagatt attcacatcc cagaaaatag
     1501 ctgccagtct gtccacctgc ggatagtaat cattgatcat ttcctcatat ttgttcttgt
     1561 tatatacatg ggtgcccaga tcggtctcct tacgatatgt cagatcagcg atcctctgtg
     1621 catcctcgtg gctggtaatg ctgatatacc gcaaaataat ggtgaatatc agaagtatca
     1681 ccgcccgggg cagcgctccg tagaacaata tcacgaacac cggatccggc tcaatgccaa
     1741 actgcagcgt ccccgccatc cactgctcca tataccagtc tctggaatga ttgcttccta
     1801 agagcatatt caaatcacag attccgtgat agaatcccgt caccatactg acagccattg
     1861 tcacgtcatt gacaacatag gtgatccaca gtgtcatccg ctccctgtac tgtaccgcca
     1921 aaagcaacgg caacacatag ataagcaccg catggaatga caaaaatgcc gcaatgactg
     1981 ccgatatcat acagatcagt gtcaaaaaca catactttac ccaatgcttt gacagatcca
     2041 cctttttata aatgtataaa acaggaattc ccatcaccgc cgacacccct gctgccatgg
     2101 taaaaattct ggcatccaca ataaagattc tcactaatgt caacagccag aggagcagaa
     2161 tagtcacaaa aatccagaaa aaacctttta gtgtgtgtat atttgagttg atctcctgct
     2221 ggtgtaattc ttttttatac ttttcctgat aattttccat cataacatcc ctatgttctt
     2281 ctatcatttg ttctcttcat tcagtgctca gcgcataaca gatacagtat aacatattat
     2341 gatttttttt gcattaaatg agcttactta gcataattaa aatcctattt ttccacattc
     2401 tgtttcatgc tgccggtgcc ccggcaatcg acctctttac tgggaaataa tcaagctgcg
     2461 gttattttcc cggtactctt ttggggaaat gccatagatt ttccggaaat tccgcaggaa
     2521 agaggagtaa tccgcaaagc cggtctgctg ccagctctca gtaatgttct tcccctccgc
     2581 catggcctgg caggcggaaa taatccgctt gttcacaata aagttatgca ccgtcatgcc
     2641 ggtgatctcc ttgaatttcc gcagaaaatg atacttactc atgtgcacgg cctccgccac
     2701 ttcctccact gtgatctcct ggctgagatg ggtctcgatg tagccggtca cctgctccac
     2761 cagaggatga tccacgatct cctgtgtgga agacaaatat tcctcccgtt tacacaacac
     2821 attcagatac ccaaaaaaca ggctcaaatt gcctctgtcc atgaccttac ggattccgca
     2881 gttctggaca tcatccccct ctaagatctc cgacatagca agctttagca gaaatccgcg
     2941 caaaatttcc tcataataaa tggggaaatg atacagtttc ggccccgctc cccagaagca
     3001 ttctgcaaga aacggctctc ccaccgccag ctcccgcagc atctgatcac tgatccacaa
     3061 aatgatccgg cgggacgaat ccattttctt ctccgtggga tgatatttgt gcatgacatt
     3121 cttatcgatc agcaggaaat ctcccttccg catccgcacc gcccggtcct cgatcaggga
     3181 agaaaattcc ccctctataa tatagatcag ctcataaaaa tcatgataat ggaacgacac
     3241 cgctccgaca ggctccccta ccttctcata gacctcatag tcaccgctca acatatattg
     3301 ccgttcgtcc gccgcatccc ggcagcgcag gctgcttttt ctgttcacga ccatccattt
     3361 tctctcctgt atcattctgt tcagttttta ttttccatgg tttatatccc ctgtcttact
     3421 atatagcaat ttttgcacta tttcaagcaa ttcatgcagt tttaatgcat ttaccattct
     3481 gctatgctta agacagcaaa aaacgaaacc aaagcattcc ctacaaggag gatttctatt
     3541 atgcaaatga cattacgctg gtatggcagc aagtacgaca cggttacctt aaaacagatc
     3601 cgccagattc ccggtgtcac cggagtcatc tccactctct acggcaccgc tcccggagaa
     3661 gtctgggaaa tggatgacat cctggcccta aagactgaga ttgaagctgc cggcttacag
     3721 ctggccggta tcgagagtgt caacattcac gatgccatca agatcggttc tcccgaccgt
     3781 gaacaatata tagagaatta tatcactacc ctggaacggc tcggtcaggc aggcatccat
     3841 atggtatgct acaatttcat gccggtcttt gactggacac gcaccgaact tgccagaatg
     3901 cgtcctgacg gttctacagt ccttgcttac aatcaggctg ctgtagacgc tattgatccg
     3961 gccaaaatgt tcgaatccat tgccggcgat atgaacggca ctgtaatgcc cggctgggag
     4021 cctgagcgta tggctcatgt caaagaactg ttcgaaatgt acaaagatgt ggatgatgaa
     4081 aagctttttg ccaacctgaa atattttctg gagcgtatca tgcccacctg tgataaatat
     4141 gacattaaca tggcgatcca ccctgacgat cctgcgtgga gtgttttcgg actgccccgt
     4201 atcatcatca acaagcagaa tatcctgcgt atgatgaaga tggtggataa ccctcacaat
     4261 ggcgttacct tttgttccgg ttcctacggc accaacctgg aaaatgatct gccggatatg
     4321 atccgctccc tgaaaggccg cattcatttc gcccatgtca gaaacctgaa gttcaactct
     4381 cctactgatt ttgaggaggc tgcacatctg tccagcgatg gaaccttcga tatgtacgag
     4441 atcatgctgg ctctttatga tattggtttt gacggtccca tccgtcccga tcacggacgt
     4501 atgatctggg atgaagtggc catgcccgga tacggactgt acgacagagc tttaggcgct
     4561 acctatttaa acggtctgtg ggaagctatc gagaagtctc ataagcagaa ataatcatcg
     4621 cacctgaaaa ctcataaatc agtttggagg aaaccattat ggaacttacc ttaaaaagtc
     4681 ttcatgacga ccgggacgca tttctcgctg ccggttatca tctgcctgaa ttcgattacg
     4741 ataccgttca taaaaacacc gtggaacatc cccactggat ccacttcggt gccggcaata
     4801 tcttccgtgc ctttcaggcc aacgtagcac agaatctgtt aaacagcggc gttctggata
     4861 ccggtctcat cgcagcggaa ggctatgact acgagatcat tgaaaaaagc taccgtccac
     4921 acgacaattt aagcattctc gccaccttaa aggccgacaa caccgtggaa aagaccatcg
     4981 taggcagcat catggagtct ctgatcctgg acagtaaaaa cgaagcagaa tacgcacgtc
     5041 tccgggagat ttttaaaaat ccttctctcc agatggccag ctttaccatc actgaaaaag
     5101 gctatgctac cgccaatgcc aaaggagaat tcttccccgc agtagccgct gacttcgaaa
     5161 aaggtcccga agcccccgaa agctacctgg gcaaggtagt ttccctgctt tataccagat
     5221 acactcacgg agcgctcccc attgccatgg tcagcatgga caactgctcc cataacggtg
     5281 acaaattata tgccgctgtc aatgcgttcg ccaaggcatg gaccgacaaa ggactggtgg
//
LOCUS       DAWDHD010000177         5321 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_887_length_5321_cov_7.576149, whole genome shotgun sequence.
ACCESSION   DAWDHD010000177 DAWDHD010000000
VERSION     DAWDHD010000177.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5321)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5321)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5321
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_887_length_5321_cov_7.576149"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1417 a         1134 c         1461 g         1309 t
ORIGIN      
        1 ggagacctta catcgagaca tgtgtatcgc atgctccggg agttcgtgga ttttgtcaat
       61 gtattcaact aactctcaat ttactctgcc agttcttccc actgttcata taatttttcc
      121 agctgctcct ggcaggctgc ctgctctttg gacagctcct gaagctttgc cacctgggtg
      181 ccgatggcgg gatcggacat ttcggtgtca atctcggaga cacgggcttc cagtctggca
      241 atctgttctt cgcatttttt caggtcgttt tctttcttgc ggagtcgtgc ctgttcttct
      301 ttctgggctt tccagtccag cttaacttcg gattctgcaa ccttagcggc aactgtgtta
      361 tctgcgtcag cactctgtgg aacactggct tctattgcag ccatcacggt gtcgtgcttt
      421 tcgagataat agtcataatt tccaacataa tttacgaatt gtccctccgt cagatccagg
      481 atcctgtggg cggtacggtt gataaagtaa cggtcatggg agacatacaa tatcgttcct
      541 tcgtaaccgt tcagggcatc ctccagaatc tccttggaca tgatatctaa gtggttcgtc
      601 ggctcatcca ggatgaggaa gtttgcgttg gagagtacca gcttggcgag agatacacga
      661 ccgcgctcgc cgccactcag atcactgata cgcttgaaca catcctcccc ggtgaagagg
      721 aaagccgcca gcacattgcg gatctgggta ttgttcaggt agggataatc gtccgagatc
      781 tcttcgaaca atgtcttctc gctgtggagc acatgatgtt cctgatcgta atagccgatc
      841 tccacattgg aacctaagcg gaaggtgccc tgatcggcag gaacgagacc atttaagatc
      901 ttaagcaaag tggtcttgcc gctgccatta tcaccgatga tcgccacgtg ttcgccgcgc
      961 ttgatctcga aattgacatc ggtaaaaagc ttatgggagt caaagctttt ggacagatgc
     1021 tccacagtca gcacatcatt gccacttaag atccggggcg tcagtgtcag cttcatatct
     1081 gtgcggactt cggagggttt ttcgatgacc tcgatcttgt ccagcatttt ctcccggctt
     1141 tccgcccgct tgatggactt ttcccggttg aaggatttta atttttcgat gacctcttcc
     1201 tgatgtttga tctcacgctg ctggttcata taggcattcc aggcagcaac gcgcagctgt
     1261 tcctttttta cggcataatc ggagtaattg cccataaagg ttgtagcctt ggactggtcg
     1321 atctcgatga ctttcccggc gatccggtcc agaaaatacc ggtcgtggga gacgatgaga
     1381 acggctccct tgtaattcaa cagataagtc tccagccagg cgatggaatt catatctaag
     1441 tggttcgtcg gctcgtccag gatgatgaga tcagggttct gtaacagaag ccgtcctaac
     1501 gccacacggg tcttctgacc gccagagagt gtggcaaccg gcttggagaa ttcatcttcg
     1561 gtaaatccca gtccctttaa cacaccgacc agctcactgc ggtagagata gccgccgccg
     1621 gtctcgaaag catgggtgag agaggtgtac tgcttcatca ggtcttccag cgcatctcct
     1681 tcggcatgct tcatattgtt ttcgcattcc cggatctttt cctccaggcg cagcagatcc
     1741 gccttgacag ataacagttc ctcgtaaatg gtgtgagagg tgtccacagt gctgttctgt
     1801 gcaaggtatc ccagggtctt gtccctcgcc agaaccacct gcccgtcatc gggagtcatt
     1861 tcccctacga tgatgcggag cagtgtcgtt ttcccggcac cgttgatgcc cacgatggca
     1921 gctttttcat gatcctctat atgaaaagag acatttttca atacctgatt ttctacaaat
     1981 gcttttgaaa tattctgaca agataatatc atggtagtaa gtatccttta tttccaaccc
     2041 acggagaccg ggctttttaa atttcattgt aacgaaaaga agaaaacctg tcaattcatt
     2101 gacaagtttt taacacatgg tgtatactaa aaaaaacgga ggtgaaagta ttggaagtaa
     2161 aagaaatttc acaggctgtg attggcagac tgcccagata ctttcgatat ttgggggaat
     2221 tgaaggatga aggtgtggaa cgtatctctt cgcaggaact ttccggactg atgaaggtga
     2281 ccgcatccca gatacgtcag gattttaata atttcggcgg tttcggccag cagggctatg
     2341 gatataatgt agaatctctg tatcgtgaga tcggtaagat cttaggactg gataagcagc
     2401 ataatttcgt catcattgga gccggcaatc tgggaagagc gctgggcaat tatatgaact
     2461 tcgaacgtcg tggatttatc ttcaagggaa tgttcgatgc gaacccggaa ctggtgggta
     2521 aggatgtccg cggtgtgaag gtcatgccca tggatcagct ggaatccttt atccgggaga
     2581 atgacatcga tatcgctgtg ctgacgattc ccaagaccag tgctgtggaa gtggcagata
     2641 agctggtggc taacgggatc cgtgctatct ggaatttcgc tcatgtggat ctgaatgttc
     2701 ccgagggaat tcaggtggag aacgtgcatc tgtccgacag cttaatgaag ctatcctata
     2761 atattaaccg ttacagttca gacatgaaat aagcaagcgt atgattgctt atttcatagg
     2821 cggagtgtct gaaaatgaat aaaaggaaaa gtcgcaaggc gatgacggat gcgaagcagc
     2881 cgcttttatg aatttcagcg caccgagaaa taatacaaca tgaagtaagg catcaaagga
     2941 gtgatattat gggcaacggt aacgcagaag aaaatgcaca ggaccgacag gctgctttca
     3001 aaaatgcatt gacgggaaag catctgcccg tactcaccct ggataataaa tggtatcggt
     3061 tgttgaataa gaccggttcc gttccgctaa aagaaacgga agatgccctg aatcagctgt
     3121 tgaaacgaca aggtaagctc aataccgagt cgaaggatat ccggaatctg aaaaagaagc
     3181 tgatgaaaga gatcgtccct atggtggatg aggcggaaca gcagggcgaa aacagtaagc
     3241 tgaacaaaca gatcgaggat cataagcgcc tgatcgagga atgcaatgag aagctggaag
     3301 catacgaaga tgagttaaaa gatatcccca gagagatcga aagaataaat cttcagctta
     3361 tgatgtttac tatggactgc tgctatgata ttatgaagga taatgacaaa cagatcaaag
     3421 agactgcgga gtgggtgggt gcgatccgta tcgaactgaa aaaacgtctg atcgaaaaac
     3481 agcagatgga acagcagaat caggagatct acaactacat gcatgatatt tttggtgcag
     3541 aagtggtcaa tctgtttgac atgaaatata atccggagca aaaatagtat gagatatctg
     3601 gtaactgcac aggaaatgaa gcaatacgat aaaaatacca tagagtacct cggcatcccg
     3661 gggccggttc tgatggaaag agcggctctg gctgcggagg attttctgaa agagcgcttt
     3721 gatgccgtga aggaacggac aaaagtgctc atttttgctg gtatgggaaa taacggagga
     3781 gacggtctgg ctttggcgag acttcttgcg gcggacggtt atacggtagc ggtacgactt
     3841 gtgggagatc cggagaaagc cacagagcaa tggaagagcc agtggcagac cctgcaacat
     3901 tttccggtga agacagacag taatacgcag gcagatgaat ataatgtgat tgtagatgcc
     3961 ttgttcggtg taggattgag ccgcccggtg gaaggcatct atgcggaagc cgtagaagag
     4021 atgaatgtgg cggaaggatt taagctggca ctggatgttc ccagtgggat ctgctcggac
     4081 accggccggg tgcttggttg tgcttttctg gcagatgcaa cgattacttt tggtttctgt
     4141 aagagaggcc ttgtaatgta tccgggaacc gattatgctg ggcaggtaca cattgcaaat
     4201 gtggggatcg gaccggagag ctttctggga cggagcccgg agatgtatac ttatgacagc
     4261 tgtgaacagc atctgccgga cagaacatcc tccgggaata agggaacctt cggcaaagca
     4321 ctgttggtag cagggagcaa cggcatggcc ggtgcagcaa tcctggcagc aagagcagcg
     4381 tatcgtaccg gagccggtat ggtaaaagtc atcaccgcag aggagaatcg gcagatctta
     4441 cagcagggaa ttccggaagc tttgtacgga tcctgcagac agctgtccga gagcctggaa
     4501 tgggcggatg tcattgctgt cggtcccggc atcggcagag aggaacaggc acgggaatgc
     4561 ctgaaaaaag tagtggaaaa gagcagaaag ccattagtgc tggatgcgga tgcactgaat
     4621 cttctggcag aagaaaacgg taaggcattg gcagagaagc tgcataccca gggagcagag
     4681 ggcagagtta tcattctgac tccccatgtg ggagaattgt ccagactgct tgcgaagacc
     4741 atcccagaat gcaagaagga gcttccggag tgtggcagag aactggcaga acggtttcat
     4801 ggtgtggcgg ttgccaagga tgcaagaacg gttgtatgta aagaacaggg agaattttat
     4861 ctgaatacta caggcaacag cggtatggcc actgcgggca gcggagatgt cctgacgggt
     4921 gtgatcctgg gacttttagc tcagggtatg aatgcaagtg atgcagctgt gaacggtgtg
     4981 tatctccatg gcagagccgg tgagctggcg gcaaagctcc atacggagta tggggtaatg
     5041 gcgggagata ttgcggattg cctgatgaaa gactatgatg agaaagaaag tgtgaagaaa
     5101 tgacaagcaa actacaggaa aagatgaatt cctaccaacg ggtatgggcg gaaatagatc
     5161 tggatgccat ttgggagaat atgatccata tgaaggaaaa tattgccccg gagacgaaga
     5221 tactggcggt cataaagacg gacgggtacg gacacggcgg tgttccaatt gccagaatgc
     5281 tggaggatgt ggattttatg ttcggctatg ccgcagctac c
//
LOCUS       DAWDHD010000178         5321 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_886_length_5321_cov_12.388530, whole genome shotgun sequence.
ACCESSION   DAWDHD010000178 DAWDHD010000000
VERSION     DAWDHD010000178.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5321)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5321)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5321
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_886_length_5321_cov_12.388530"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1396 a         1140 c         1523 g         1262 t
ORIGIN      
        1 gatccaggcg gccatgaagg aacgccgggc ggaggtaaag gaagtcgttc tgacggatgt
       61 atgctccttt acattgggaa cggaagtcgt ggtggaatac gaggaaggca aatttgaaga
      121 cggccgtttc tgtcccatca ttgagcgcaa cactgtgatc cccgccagtc atacggagcg
      181 gttgtacact gtcagagaca atcaggacaa ggtgcgcgtc cgggtactgc agggagagag
      241 ccgtttcgcc agaaataatc tgtttctggg agaactgaac attgatgttc ccaaagggcc
      301 caggggcagt gaagccgtgg atgtgactta cacctatgac atcaattccc tgctggaagt
      361 ggaagtcaag gtggtatcca ccggactgac ccagaaaatg atcatcaagg gacaggacaa
      421 ccagatgacg gatgatgaga tccagaagcg aatggaagaa ttgtcctacc tgaagatcca
      481 gcccagagac ctggaggaga accgtctggt gcttctcaga gcggagcgga tgtatgagga
      541 agcactggga gaccggagaa aagaactgga tcgttatatc actgttttcg aggctgcatt
      601 gaaaaagggc ataaaagagg agatcgagga ggccagagag gcattgaacg agatcctgga
      661 agacgaggat gagtgatgtt ttatccttga cgcggcgaaa cgataacatt ataatgtaga
      721 aatgtaggtt tattttccga aaggtgtaat ttttagacaa ggagaccgag agaatgagac
      781 aattcacttc tgatgagctg agaaaagctt ggaaacaatt ttatatagac cgtggccatg
      841 tggatgtggg agcagtatcc ctggtatctg acggatccac cggtgttatg ttcaacgtag
      901 cgggcatgca gcctctgatg ccttatctgc tgggacagaa gcaccctctg ggtaccagac
      961 tctgcaatgt gcagggctgc gttcgtacca acgatatcga ttccgtaggt gacaagagcc
     1021 atgtgacttt cttcgagatg atgggaagct ggagcctggg agattatttc aagaaagagc
     1081 gttgccagtg gagtttcgaa ctactgacac aggtattcgg atttgatgcc gaccatctgg
     1141 ctgctactgt atttgccggt gacgagaatg cacccagaga tgaggaaggc gcacactacc
     1201 gtatcgcttc cggtttcaag aaggagaatg tatattatct gcctgccgat gacaactggt
     1261 ggggactgga atacggcccc tgcggtcccg acagtgagat gttctatatt gcagacagac
     1321 cggactgcgg accgaactgc ggacccggct gtgactgcgg taaatacacg gagctgggta
     1381 atgatgtatt tatgcagtat gagaagcatc atgacggtca tctgaccccc ttaaagcaga
     1441 agaacgtgga taccggttgg ggtctggagc gtattctggc ctttttaaac ggtaccagag
     1501 atgtatacca gacagacctg tttgccccgg tgatcgctta cattgaagag gcttccggtg
     1561 tgaaatacaa tgcggatgaa aagctgacca gatccatgcg tattctggca gaccatctgc
     1621 gtaccagtgt aatgctgatc ggtgacgaag caaagctgct tccttccaat accggtgccg
     1681 gttatattct ccgtcgtctg atccgtcgtt cggtaagaca cggacgtacc ctgaatatga
     1741 ctacagagca gctgctgcac attgctgcca tgtacattga tgagatctat gcagaaagtt
     1801 atcctctgat gaaaaagaac agagagttca ttctgagcga actgcagaag gagatcgcac
     1861 gtttcgagag cactctggag aacggcatga aggagttgca gaagattctg gagcagaaga
     1921 gaagtgaagg taaaaaggag atcgacggca agtccgcatt ctacttatat gataccttcg
     1981 gattccctct ggagctgact gtagagctgg cacaggaaga gaatctgacc gtagatgagg
     2041 agggctttgc cgctgccatg gaagagcaga agcagaaggc aagagaagga cagaacttct
     2101 cccagaagat taccaccgca gcaggtgtat tcgatggact ggatgacaag atcaccagcg
     2161 aatttgtagg ttatgatgcg ctgaccgcag aaggaaaggt agtagcactg gccaccgaga
     2221 ctgaactggt aaacacttta aagataggtg agaccggtac actgatcacc gatgtgactc
     2281 cgttctatgc taccatgggc ggacagaagg gtgactttgg tgtgatccgc acgaaaaacg
     2341 gtacttttga agtgactgag accgtgaaga tcgcaggcgg acgcatcggg catgtgggta
     2401 aggtcgtatc cggaacagtt actgtgggag acaaggcaga gctggcagtg gataccgaca
     2461 acagaaagag cgtatgtaag aaccattccg caacccatct gttacagaag gcactgcaga
     2521 tcgtactggg tgatcatgta gaacagcagg gttcttacca ggacggcgca agaacccgtt
     2581 ttgacttcag ccacggacag gctatgactg cagaagagat cgctaaagta gaggctctgg
     2641 tcaacgagaa gatcgcagag gatattgcag ttgttaccga tatcatgagc atcgaggatg
     2701 cgaagaagag cggagctatg gctctgttcg gagaaaaata cggcgatact gtccgcgtgg
     2761 tatccatggg agacttctcg aaggaactgt gtggtggtac tcatgtaaag cataccggtg
     2821 agatcggtta cttcaagatc ctgtccgaga gcggtgtcgc tgccggcgtc agaagaatcg
     2881 aagcactgac cggtgccaac gtgatggcat actatcaggc tatggaagag aacttcaata
     2941 aggcagccgc agccgcaaag gcagctccgg cagccctgac cgagaagatc cagcatatgc
     3001 aggcagaact gaaggctctg aatgctgaga acgaatccct gaaagccaag atggcacagt
     3061 ccgcactggg caatgtcatg gatcaggcag tggaagtcaa gggaacaaag cttctggcaa
     3121 ccagcgtaga cggtgtggat atgaatggct tgcgagacct gggcgaccag ctcaaggata
     3181 agcttggcga aggcgttgtc gtactgctct ctgcacagga tggcaaggtc aatatgatcg
     3241 ccatggcaac agacggtgct gtaaaggccg gtgcccatgc aggcaacctg atcaagggaa
     3301 ttgccgcact ggttggcggt ggcggcggcg gacgtccgaa catggcgcag gccggcggta
     3361 agaatcccgc aggcattccc gcagcgatcg cggaagcgtc caaagtactg gagggtcagc
     3421 tcgcataatg gaacgagaga atctgaaatc ccgtttaggc tttatattgc tgtctgccgg
     3481 ctgtgccatt ggtatcggca atgtatggaa gttcccgtac atggccggcc agggcggcgg
     3541 tggcgcattt gtattgtttt atctgatctt cctggtgatc cttggtctgc cgattatgac
     3601 catggagttc gctgtgggaa gagcaagtaa gaaaagtccc gtaaaggctt accaggcact
     3661 ggagaagccg ggacagaagt ggcatatcca cgggtatttc acgctggcag gctgttatct
     3721 gttaatgatg ttctatacta ccgtagcagg ctggatgtta cactattttt atatgacggc
     3781 tgccggtaaa ctttccgggc tttccgcaga tgcggtagcg gatcagttta cccggatgct
     3841 ggcagatcca ggggtcatga tgttctggat ggtactggtg gtagtcatcg gtgtcgtcat
     3901 atgtgccggt ggcctgcaga acggtctgga gcgtgtgacg aaagtcatga tgatcgcact
     3961 gcttgccatc atggtagtac ttgccatcaa cagcttcttc atggcgggag caaaggaagg
     4021 tctgaagttc tatctggtac ctgattttgg acgtatgcag gaggttggtg tggtatctac
     4081 actggtaggt gccatgaatc aggcattctt taccttaagt cttggcattg gtgccatggc
     4141 gatcttcgga agctacatcg gcaaggatca ttccctgatg ggtgagtccg tcagagttgt
     4201 ggtactggat actttcgtgg caattaccgc aggactgatt attttccccg cctgctttac
     4261 ctatggggtg gatcagactt ccgggcccag cctgatcttt attactttgc cgaatatttt
     4321 cgcaaatatg ccttacggcc gcctgtgggg aagcctgttc ttcctgttca tggcgttcgc
     4381 agctctgtct acagtactgg cagtatttga aaatatcatc tgctgtggta tggaactgac
     4441 cggtgccagc cgcaggaaat cttccctggt gaacctgttc ctgatcattg cattgtccgt
     4501 gccctgcgta ctggggtata atgtgtggag ctgggatggt tttgctgtct tcggcggagc
     4561 ggtactggat tttgaagatt tcctggtaag taatctgttc cttcccttag gatccctggt
     4621 atatctgttg ttctgtgtga ccagatacgg ctggggatgg gagaactata aggcagaagt
     4681 aaataccggc aagggcttaa aagtccacga ctggatgcgc ggttatctga cctacggact
     4741 gccactgatc gtgatcttta ttttcctgtt tggaatttat gataagtttt ttaaataaga
     4801 ctataatatg atgtcggaat cacccccccc gactttgatg cctacggatt gttccgtgct
     4861 acgcactccc acaatcctac cctctattcg catatcgtgg gattatcatc ccacttttat
     4921 gctcatatcg gggcgggtca taaggtcttt cacccactta tgagcaagct catatgggct
     4981 tagacctttg accctggcat cataatataa taaaaaatta aaaaagtagt tgacgaatgt
     5041 attttttatg ctataatatc aaatgtgtgt agaacaactg cgcattagaa taacccagtc
     5101 agtcatgtta cttgaaggga ctgaagggag gtcgggtgta agatgtcaaa cgtaatcgta
     5161 aaagagaacg agactttaga tagcgcttta cgtcgtttca agagaagctg cgctaaggct
     5221 ggtatccagc aggagattcg taagagagag cattacgaga agcctagcgt aaaacgtaag
     5281 aagaagtctg aagcagcaag aaaacgtaaa tataactaat g
//
LOCUS       DAWDHD010000179         5301 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_891_length_5301_cov_11.267823, whole genome shotgun sequence.
ACCESSION   DAWDHD010000179 DAWDHD010000000
VERSION     DAWDHD010000179.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5301)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5301)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5301
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_891_length_5301_cov_11.267823"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1318 a         1410 c         1186 g         1387 t
ORIGIN      
        1 accagagtca ggcacacgga tgctgccacc agcaccccta ctttatccaa taacagtaat
       61 tttgcaaagt tcgctgcgga aagtgtaata ccgcagatca gtgccacgcg gatctccttg
      121 aatacgacct tcggcacatc ccggtattcg atctcattca gggacagtcc acggatgacc
      181 gtaacgctcg cctggcttcc ggcatttccg ccggtatcca tcagcatggg aatatatgcc
      241 accagtaccg cacacacgct cagcgcatct tcaaaagaag aaatgatagc tccggtaaat
      301 gtagctgaca ccatcagaag aagcagccag ggaatacgct ttttgaaggt ctcaaatacc
      361 ccggtcttca tataaggctt atcactgggc acgatagccg ccattttttc catatcctcc
      421 gtggtctctt cttcgatgat atccacgata tcgtccacgg tgataatacc taccagccgg
      481 ttctcattgt ccactaccgg catggctgta aagtcgtatt ttttgaactg tctggcaact
      541 tcctcctggt ccatcagggt attgatggag atgacatttt catgcatgat gtcaccgatg
      601 atctcgtcac cgtccatgag cagcagataa cgcagggcca cggttcccac cagtcttctc
      661 tgcgcatcca gcacatagca gatattgatg gtctcactgt cgaggcctac cttacggatc
      721 cgctcgatgg cctgattcac cgtcagattc tccttcaggt ctacatactc gaccgtcatg
      781 atgcttccgg cggaatcttc cgggtaccgc agcagatgat taatgtctct gcgtacctca
      841 gggcttgcgt tggtcagaag ttttttgaca atgttggcag gcatctcttc cagcaggtca
      901 gccgcatcgt ctgccatcag gttgttgatg atgttcgttg cttccttatc tgacagggag
      961 gtgatgatct tctgctgatt gtccatatct aaataggaaa aaacgtctgc cgccaggtcc
     1021 ttgggaagaa tgcggaagac tttgagcata tcctcttctt ccagttcttc cagaagtcct
     1081 gcaatatcgg catcgttgag ttcagccaga tactggcgca gtttggtata ctgcttggtg
     1141 ttaagaagct ctaagacttc ttccagttct ttgatctgtt ccatgatgat cctccttatg
     1201 atgttttcta tgggtgtaac ttatgcagta acttcgcccc ggaagtgggt tgtcagaact
     1261 cttctgtgta cacttcataa aaaccaccac ctttctgtag gatccatcca gaaccccatt
     1321 cgcaaaatcg cctctacgag gctttccttt tgctcttgac atatattctt acgaatgtaa
     1381 acattcgacg aatatccgtc aatatttaat ggttctgaat tgttaataat attttaactt
     1441 tatgactatt ctttgtcaat gaaataagtt tctttcaatt tctcaatttt tacctttccg
     1501 caggtggttt ctgtcaggtt tttcctgaga ctatcctctt tttccaccgg gacaagtaaa
     1561 ataaatttta cgttttccgc atagatgctg tccgcaggaa cgatgccctg ctgtccgaaa
     1621 tagtactgca gctttccaac atcattataa tccgttgtca cttccagctc gcagcccttc
     1681 cgcataatgc cggtctcaca atgatccagc ccctccttca ccgcctgggt ataggcccgc
     1741 actaggccgc cggtgccgag caatgtgcca ccaaaatatc tggtcactac caccgcaata
     1801 ttgcggatac cggatccggt caatacttcc agcatcggac gccccgccgt tccggagggt
     1861 tccccgtcat cgctgcaccg ggtcagttcg cctctgcttc ctattataaa tgcagaacag
     1921 ttgtgtctgg catcccagta ttttttcttc atttcttcta taaaagccac tgcttcttcc
     1981 tcggtttcac acttgcggat cgtagagata aagcgggact ttttctccac gatttcgcct
     2041 tccccgccgg acaaaaggac tctatagtta tctgttgctg tcatgtcacc catgtatggc
     2101 ctcacttatt tttaaattct tattttcttt tgctttcttt ttcagtgtag cataacttct
     2161 ccaaaatgtg aaacaattct aaaaagcctt tatttacata gcattatttg gtataatgtt
     2221 ttcgtctgta tctcagacag aaaggacttg ttttatgaat tatagtaaaa aaggtgtccg
     2281 tgcgaagcaa aagacactca actctaaatc acaaaaatgg ggaaggaaaa ttgctctgac
     2341 ctgtgtcaag gtcatgctag ccgctgtcgt aggcatcggc atctgcggtg tagccgccgg
     2401 tatcggtgtt ttccgcggta tcctctcctc cacccccacg atccgtctgt cggatgtcgt
     2461 ggctgtcggc gaagctacca tcgtctatga cagagagggc aacgagattg accagtacgt
     2521 cagcaccaac tccaaccgtc tctccgtcgg catggacgag attcccgact acctgggcaa
     2581 ggctttcgta gccatcgagg atgaacgttt ctatcagcat aacggtattg atttcaagag
     2641 tattatccgt gccggttatc agttttttaa gaccggtggt gaagaagccc agggtgccag
     2701 taccattacc cagcagcttc taaaaaatac cgtctttacg gactggacct ccgagggtaa
     2761 taacaagatc aaaaaaatca agcgtaagat ccaggagcag tacctggcac tggagattac
     2821 aaagtactac tccaaggacg agatcctgct gcgttacatg aacgccatca acctggggca
     2881 gaacaccctg ggtgtggaat ccgcatcctt acgttacttc ggtaaacact gcagtgaact
     2941 gaccatttcc gaatgtgcgg tcattgccag catcacccaa aatccatcca aatacaatcc
     3001 gatccgtcat ccggaagaga atgtaaagcg tcgtgagaaa tgtctgaata aaatgctgga
     3061 gcttggcttt atcagtcaga cacaatacga tgaagccatg gcggataccg atgccgtgta
     3121 cgagcgtatt ggtctgtacg acattgacta tcaggaagcc aacgccacta ccggttccta
     3181 cttctctgat gcggtatatg agcaggtaaa acaggatctg atcctgtccg gttacaacga
     3241 gaacatggcc gagactctgc tgacctccgg cggattgcgt gtggaatcca ctctggatcc
     3301 caagattcag gcaattctga acgaagaata tgcagatccc tccaattatc cggagaacgt
     3361 gaaatggtat ctgaactatg cacttaccat tatctctcct gacggcacaa aaaataattt
     3421 cagcaaggaa aatatgatga cctggttcaa gcagaaccag agtgcgaagt tcaatctgat
     3481 cttctcttcc caggacgatg cctatgcggc cgtagacact taccgttctg caatgctggc
     3541 gcagctgggt gtggaagaca atgcggacaa ttacgaagaa actatctcca tgactcctca
     3601 gccacagtcc gctatggtca tcgaggagca ggacaccggc tatgtggtag ccatgatcgg
     3661 cggccgcggt gccaaggagg gccgtagaac cctgaaccgc gccaccagtg ccaagcgtct
     3721 gccgggatct accttcaagg tagtggcctc ctacgctccc gctctggaca gtgccggcaa
     3781 gactctggct actgtataca acgatgcacc gtttaactat gcagacggta ctcccgttcg
     3841 taactggtac aagaccggtt accggggcat ccagaatatc cgaagcgcga tccgtgactc
     3901 cctgaatatc atcgcagtta agaacatcac ggtgatcact ccgagacttg gttatgatta
     3961 tctgctgaac tttggattta ccactctgac cgacggtgtg caggtcggca acgaaatcaa
     4021 gtccgacgtg aaccagtccc tggctctggg cggtctgacc tacggtgtta ctccttacga
     4081 actgactggt gcctacgcag ctattgccaa cggcggtact tatgtcaccc cgaagctgta
     4141 caccagagtc accgattccg atggcaatgt aattctggac aacaccaatc cggctaccag
     4201 acaggtcatc aaggagacca ccgctttcct gctgaccagt gctatgcagg atgtagttac
     4261 ctccggtacc ggtggccgtg taaatttcgg cggtatggct atcgccggta agaccggtac
     4321 tactaccgga cctaccgatg cctggtttgt aggatatacc ccttactaca ccgctgctac
     4381 ctggaccggg tacgacaaca atgttgacct aaacaacgct gaggacggtg tctccaagac
     4441 cttatggcgt aaagttatga agcgtgttca tgaggatctg cctaacaccc agttcccggt
     4501 tccctccggt attgtacagg tagcggtatg ttcccagtcc ggcaagctcc cgattcccgg
     4561 tctctgcgat ggctgtgtat atacagagta ttttgctgag ggtacggaac caacggaaag
     4621 ctgcgatgta cactatcagg gcgagatctg tgcttacgac ggattacccg caagccctga
     4681 ctgcccattc aagtacaccg gtgttgccac catgcctctg gtagaagatc ctgcattaca
     4741 gcagggctcc actgtcatca tcaacaatcc ggatggcacg cagactgtca gcacaccgaa
     4801 caccagaagt cagtgtcagc atgatgcaac cttctttgcc aatccggact acgaatctgt
     4861 gatcaaccag caacaggccg agatcaatgc ccgtaatgct gcagcgcagc cgcagacaga
     4921 agaataatat gtgaaccata gaataccata gaaataacaa taccagcccc tgtaccactg
     4981 ctccacgcag ctacaggggc tggttatttt ttgcataaaa aaatcaccgg atcaaacgtt
     5041 ttcacattcg atccggtgct catgatatac cttatgtttt cagatgttcc tatacaatac
     5101 cctgtgcagt catagcatct gcaaccttca agaagcctgc gatgttagca cctgctacat
     5161 agttaccttc catgccgtac ttcttggctg catcatccag attgtggaag atattaacca
     5221 tgatggtctt cagctttgca tcaacctctt cgaaggtcca ggaaagtctc tcactgttct
     5281 ggctcatctc cagagcggag g
//
LOCUS       DAWDHD010000180         5167 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_917_length_5167_cov_8.615415, whole genome shotgun sequence.
ACCESSION   DAWDHD010000180 DAWDHD010000000
VERSION     DAWDHD010000180.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5167)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5167)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5167
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_917_length_5167_cov_8.615415"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1461 a          990 c         1473 g         1243 t
ORIGIN      
        1 ctggaaagcc tctctcacaa attgctggag ctggtgggaa tggatagaca ggaattagaa
       61 ttcaagaagc ttcagacgaa agaactggag gaaaatattc gggatacgat gcggatcccc
      121 tttggacgta aaaggatccg ggggaagatc gatctggaaa gaggagtgat ctggggggac
      181 agggatctgc ttctgtcact gttatataat ctgctggata atgccgtaaa ggctgtggaa
      241 gaaggcggat ttattctgat gaagggaagt aagcgcacac agggatatga gatcaaggtg
      301 gtagacaacg gcagggggat ccctcaggaa gagatcgcga ggatcacgga ggctttttat
      361 atggtggata agtccagatc ccgcaaggag ggcggagccg gtatcggaat ggctctctgt
      421 cagaagatca ttaccctgca tcagggggaa ctgaagattg acagcaagct tggagaaggc
      481 acggtaatgc ggttatattt tcccggggaa atacatgctg taaaaaataa aagcaacagg
      541 ggagcaaaag gcggaatatg aaaaaacaga agaataagaa ccggcgcaat ttcctactat
      601 gtatgggcgg actgctcgtg ctgatcgtat ttgcattcgc aggaccggaa gggctgttac
      661 gattgcagga taaaagcagg ctgaccaggt cagtcagtat atcaaataca ggaacggatt
      721 ataccgtctg gaaggaagag tatgagacag acaccgtgcg cagacttcaa aatctggcag
      781 acggtatcgc tttgggaaaa cagtactata tctctgaaca ggattatgaa gctgcaacgg
      841 atggtgaaat atatagtctg atcgatgaat atattttaaa tacggaatgg aattacactt
      901 ttcaggatat gggtgtgatc ccggaggatt attttttcaa aaatatggat acagatcata
      961 tttccatcaa acgatatatt ttgtatgatg aaaagctgtc ggacggagtc gcaatcatgg
     1021 cctatgacat ctgtatgcgc atgaaggaag aggaggttac gggagtcagt atagagtgtg
     1081 tcgtggattc cgagaccgga acgctttatg ccatgaaggt tgtattggaa gatgagacag
     1141 aacctgtcac ctgggatgat atcggtgcgg atgcccctta ttattttatc accgattata
     1201 tgcagtctta tctgcatgat tataattccg ggacctatat tgaaatcgaa ggagaggcgc
     1261 cttatgatac caatgcacag atcatgtggg ataaagaagc tgaaataaag gctggaatag
     1321 aagcggagga ccggacgacc atggaaaacg acaacggtga gtattcgtgt gatcttgcat
     1381 acggagagca taagctgcat ctgaccgcaa cactgctcaa tgcttccaaa ggacaactgg
     1441 agaaaggcat cggatatctg ttcgggatcc gtgagatcgc agaaaagata ccgggttttc
     1501 tgccggatat ccagtaaata cattccggaa aatacaggac tccggaaaag cagccggaca
     1561 gtgatagcaa cggtataaaa tagaaaaata aagaatatat tttagaaaat aagtcgggga
     1621 gatacgactg tttatgatca caggaataaa aaaactttta cagcaggcag accggatcat
     1681 atggggaccc tggcttattt ttttgttgct gggaaccggc tgttatctga tgtttagctt
     1741 acgatttctt cccttgaaga acctgcctgc tgcactcagg cgtgtgtttt tgccagaaag
     1801 ccggaaggga acagagggca ggggagtatc ttcgttttct tccctgacca cggaactggc
     1861 ggccaccatt ggaacgggaa atattgtagg ggtagccact gccatggtac tgggaggacc
     1921 gggtgctctg ttctggatgt tactgtccgg gatcatcggg ctgtcgacca aactggtaga
     1981 aagcacactg tgtgtcaggt atcgtgtcaa aaaccaaaag ggggaaccgg caggcggtcc
     2041 gatgtatgta ctgcaaaatg cattcccaca gaaaacggca ggcaggatcc tcgccatgct
     2101 gtttgcagca tttgctgtgc tggcttcctt cggcatggga aatatgacac agggaaattc
     2161 tattgcggag gcactttcgg tgacgtttca ggtaaaacag acagtcacgg gaattgtgtt
     2221 aagtctgctt acgattctgg tgattctggg agggatagga accattgcga aggtgacgga
     2281 atatctggtg ccctgtatgg cagtgtttta tctgtttgga acgggaatgg tgatctttac
     2341 ccattttaag aatcttcccg ccggtgtggt gcagatcctg tggggagctt tctgcccgga
     2401 ggctatgaca ggaggggcgg cagggacgat gcttgcagtg gaaaacggaa tcgcccattc
     2461 cggaagaatg gcgatgcgct atggggtctc cagaggtgtt ttctcaaatg aagcgggact
     2521 gggggcggcg ggaatcagtg ctgctgccgc agatacgtca gatgcggtgc atcagggata
     2581 tatcagcatg acgggtgtct ttattgatac cattgtgatc tgctctctga cggggcttgc
     2641 cattgcggcc agtggaatgc tggggcagag ggacccccgg ggagaagtcc tgaacggtac
     2701 agccctcatg atcgctgttt tttcagatac tttcggcaga acaggggaat ggatgctcac
     2761 aatctccatt gtactgtttg catttgccac gattatcgcc tgggaatatc agggggagaa
     2821 agcttttgag tatctgacgg gcggttgtga ccgaacccag tggtatcgcc tgggctatgg
     2881 actggtcact tttttgggag cggtctgctc gctggaagcg gtgtgggatt tttcggatat
     2941 ttgtaacgga ctgatggcag taccgaatct gctggcggta ctgatgctgt caagagggat
     3001 ctgtcgggaa atacggaaat ataatttccg agaaatataa aatttgaaat taatgattga
     3061 agggttgccg acaccatgat atgataaata aaatacagct gtccacgttt ataagagcgt
     3121 aagagaggac agttgtattt gtgtatacga tgagccaggt acaggttcgt gcttgcacaa
     3181 ggatctgtat ttggcgaatg tacatcatcg agcttttaag cgagatggtg tataagttaa
     3241 ggagtgagat tatggacaag accattgaaa attttttacg atatgtaaag atcgataccc
     3301 agtccagtga agaaagcacc accatcccca gtaccatgaa acaacatgat ttggccagac
     3361 agctggtgag cgaactggag gccatggggg ccgcagagat cacttatgac aaggagcact
     3421 gttatgtcta tgccacaatt ccggcatccg caggctgcga gaaagctccg gtacttggct
     3481 tcatttccca tatggatacc tctccggcgg tgacggatac caacgtaaat ccccggattg
     3541 tagagaatta cgatggaaaa gatattgtgt tgaatgcagc agagaatatt gtcatgaagg
     3601 tggaggattt tccggaactg ttacattata tgggacaaga tctgatcgtg acggacggaa
     3661 cgactttgct tggcgcggac gacaaggccg gtgtggctga gatcatgacc atggcggaga
     3721 ctttattaat gcatccggag aaaaagcatg gtaagatccg gatcggcttt accccggatg
     3781 aggaagtggg tgccggagcc gatcattttg atgtgaagct gttcggtgcg gattatgcct
     3841 acaccgtgga cggcggagcg ctgggggaat tggaatatga aaactttaat gcagccggag
     3901 caaaactcca tgtctatggc agaagtgtgc atccgggttc cgccaagggt aaaatgaaaa
     3961 atgccattct gatcgcgcag gagttccaga agctgttgcc ggtggagcag aatccaatgt
     4021 ataccgaagg ctatgaggga tttttccatc tggatgccct aagcggcaat gtggaggaaa
     4081 ctactgcgga ctatattatc cgtgatcata acagacagct gtttgaacag aaaaaagagc
     4141 tgtttatgtc ctgtgcggat ttcctgaact ggaaatacgg agaaggtacg gtcgtagtcg
     4201 acatgaagga ttcatattat aacatgcggg agatcatgga gcagcacatg catctgatcg
     4261 acaacgcgaa agctgccatg gaagaactgg gaatcgagcc caaagtatct ccgatccgcg
     4321 gcggtacaga tggagccaga ctgtccttca tggggctgcc ctgtccgaac ctgtgcacag
     4381 gtggagagaa ttaccacggc agatacgagt acgcctgcgt acagtccatg gagaagacta
     4441 cggaactgct gatcgccatt gcagcaaaat atgcgaatcg ataagatgag aacaaaaact
     4501 gatttggaaa ggcaacattt ttttatatga cgaatgtaac acaaatgaac gaaacggaga
     4561 gacagtatta ttttatggag aaggcctccg gtcatgtggc aaagcttgga gaaaagctgg
     4621 gcagaaagcc gacctgctgc gtgacgactt tcgggtgtca gatgaacgcc agggattccg
     4681 agaaactggt gggaattctg gagaaagtgg gctatgagat cattgaggac gaaaatgcgg
     4741 atttcgtgat ctacaatacc tgtacggtca gagacaatgc aaatcagcgc gtttacggaa
     4801 gactgggggt actgaacggt tacaagaaga aaaatcccca tatgaagatc gccctgtgcg
     4861 gctgcatgat gcaggaacct tccgtgatcg aaaagatcaa gaccagttat cgttttgttg
     4921 atttgatctt cggaacccat aatattttca aatttgcaga gctgttatgc tccctgtttg
     4981 aaaatgaggg aatggtcatt gatatctgga aggatacgga caagatcgtg gaagacctgc
     5041 cggtggagag aaagtattct ttcaaatccg gtatcaatat tatgttcggc tgcaataatt
     5101 tctgcagcta ttgtattgtc ccctatgtcc gcggcaggga acgcagccgt aatccgaagg
     5161 atatcat
//
LOCUS       DAWDHD010000181         5166 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_918_length_5166_cov_10.060067, whole genome shotgun sequence.
ACCESSION   DAWDHD010000181 DAWDHD010000000
VERSION     DAWDHD010000181.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5166)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5166)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5166
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_918_length_5166_cov_10.060067"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1472 a         1057 c         1416 g         1221 t
ORIGIN      
        1 gagacgcagt ttggcgagga agtagacaca tatgcggact gtgtgtatga gaaagcggat
       61 cagctgcttc gggagaaaat tcaggaagga aaatttgtac aggatccgac gccatgtttc
      121 tatctgtatg aactgaccat ggacgggaac agccagaccg gtgtagtggc ttgtgcctct
      181 attgacgatt accggaacaa tgtgatcaaa aagcatgaaa ataccagagc ggataaggag
      241 gaagaccgta tccgccatgt agatacctgc agtatgcaga cggggcccat tttcctggca
      301 tatcgggcga aagaggaact gcagaacaag atcggagaga tcaaaacaca gacaccggca
      361 tacgattttg tcagtgagga cggcatcgaa catcgtgtgt gggtgatttc ggaggctgcg
      421 gatgtggcaa tgattgaaga ggcgtttgca aagatcccgg ctatttatat tgcggacggt
      481 catcatcgtt gtgcctcagc ggtaaaggtg ggattaaagc gcagagagca gcatccggat
      541 tatacgggag aagaggaatt caattatttc ctgtcggtga ttttcccaga tgaagaactg
      601 cagatcctgg attataaccg ggtggtgaag gatttaaacg gtatggatgt cgatgcgttc
      661 ctggcagcca tcgaagaaca ttttatgata gagaaaaaag gacgtattcc ttaccggccg
      721 gagaaaaaag gagctttcgg tatgttcctg gaagatgagt ggtactgtct tacagcgaag
      781 aaggagattc ggagcgaaga tgctgtggaa ggactggatg tatctctttt acaaaatcac
      841 ctgttgaatc ctgttctggg aatcgaagat ccaaagaccg ataagcggat cgattttgtg
      901 ggaggtatcc ggggacttgc ggaactggag cggagagtac ataccgatat gaaggtggca
      961 ttttccatgt atcctacttc cataggggaa ctttttgcag tggcggatgc gggacgtctg
     1021 atgcctccga agtccacctg gttcgagccg aagctgcgca gcggtctgtt tctgcatgag
     1081 atagagcggt aatacctacg accggataag caaaatagtg acagaataga atgcagcaga
     1141 ctacacgccg cagaaaagag ggaatatgac aaacaaactt tataaactga tgaactgggc
     1201 aaagatagaa gaaatcactt attctgaatg tgataatccc catgaactgt taggacctca
     1261 taagcaggga agtaaaatgc tggtacaggc attcttcccg ggagcagaaa aggtgacgat
     1321 ccactggaag aagacaggac aggtaggcga agcttttctg gcagatgtcc ctatggaact
     1381 ggcagacgaa gaaggatatt tcgcagccct gataaatgcc gggaagatgg caccttatga
     1441 atatgtggtg gaatatggaa agacagaaga gcgtgaagcc cacagaatga tctgcggcga
     1501 tgcttaccgg catgttcccc agatcacgaa ggaagatatg gatcgttttg cagtcggtgt
     1561 tcattacacc atttatgaaa aactgggcgc acatcccatg gaactggacg gagagagtgg
     1621 aacctatttt gccgtctggg caccgaacgc catgcgcatc agtgtagtgg gggatttcaa
     1681 cggatgggac ggacggatcc accagatgag acgcctggga gattccggta ttttcgaatt
     1741 gttcattccc ggcgcagaag caggaaactg ctataaatat gaattgaaga tcaaaggtgg
     1801 actgacttat ctgaaagcag acccttacgg taatgccgcg cagaagagac cggagacagc
     1861 ttccgtcatc gctgacctgc gagatttttc gtgggatgac agcgaattct tgaaaaaaag
     1921 ggaaagctac cagtgcggaa atgctccggt cagtgtgtat gaaatgtatc tgggaagctt
     1981 tgtgacgccg gacgagggac aggattatgc gaactatcgt gagatcgctc cgaaggtcat
     2041 tgactatgta aaaaaaatgg gatacaccca tgtggaactg atgccggtta tggaacatcc
     2101 tctggatgct tcctggggct atcaggtgat cggttattat gcccctaccg ccagatacgg
     2161 tacaccggag gactttatgt tcttcgtgaa tgaactgcat aaggcgggaa tcggcgtgat
     2221 ccttgactgg gtaccggcac attttcccag agatacttac ggattgtccg gctttgacgg
     2281 cacctgtctc tatgagcatc aggatcccag gcagggaagc catcctcact ggggaacgct
     2341 gatctacaac tacggccgtc cccaggtatc caattacctg atcgccaatg ccttattctg
     2401 ggtggaaaaa tatcatgcag acggtatccg gatggatgcg gtagccagta tgctttattt
     2461 ggactacggc aaacaggacg gggaatggat tcccaatatg tacggtggta atgagaatct
     2521 ggaagccatc gagctgatca aacactttaa ttcaatcctc aaaaagagag atcccggggt
     2581 attgtccatt gcagaggaaa gcacggcatt tcccatgatc accggatcac tggaggaagg
     2641 cggactggga tttgacctga agtggaacat gggcttcatg aatgactatc tggattatat
     2701 caaatacgat ccctatttcc gcagccacca ccacggagag cttactttca gcatgatcta
     2761 tgcctacagt gagaagttca tgctggtatt ttcccatgat gaagtggtac acggcaaagg
     2821 aactctgctt ggtaaaatgc ctggagacag ggcacagaag cttgctaatt tacgtctgac
     2881 ttacggttat atgatgaccc atcccggcaa gaagcttctc ttcatgggac aggatctggc
     2941 ggaagagaaa gaatggaacg aaatgcgtca ggtagagtgg cagctggagg aacagaagga
     3001 aaatgccggt gtccagaaac tggtgaagga tctgaatgta ctttacagag agaataaggc
     3061 attgtatgaa tgcgatgatc aggccaaagg cttccagtgg atgaacgaga tctctgccaa
     3121 tgaatgctat gtaagctttg tgcgtaaggg agaagaggca gaggaactgc ttctggtcgt
     3181 agcaaacttc tccggagtac ccagagagat caccacaggt gtgccttacg agggaaaata
     3241 caaggagatc ctgaacacgg atgctgtgca atatggcggt accggtgtgg tcaatgaccg
     3301 tgtgaaacgt gcggaggacg tggaatggga tgacaaaaag cagtccgtta ccgtgaaaat
     3361 ggctcccctt tccttaagca ttctgcagtt tattccttat accgaggcag aactggaaaa
     3421 agtgatcgag aagcgtatcc gcaagaatac accgatccgc aagaccgcag gcaagactac
     3481 aaaaagaaaa caggagaaat aaggcaacat ggatctgaca gataacgtaa ttgaagaggt
     3541 gcagaaaaaa gaaggactga tcgcatcttt cctggatacc ctgccggaaa aggcactgaa
     3601 tcttggcatc cggatcggac tggcactggt attctttttc attggaatgc agctgatcag
     3661 gctgatccgc aagatcatcc gtaaatctat gaaccgggcg ggagcagaga ccggtgtgat
     3721 ccagttcgtg gactcttttg taaaagcttc tttatatata atactgatcc tgacactggc
     3781 gtcctccttc ggcgtggacg cagccagcat tgtggcgttg ctgggatcgg ccggcgtggc
     3841 catcggtctg gcagtgcagg gaagtctgtc taatctggcg ggcggtgtgc tgattttatt
     3901 attgaagccg tttagggtgg gagattacat tgtggaaggc tcctccggta aggaaggtac
     3961 agtaaaagag atccagatct tctatacaaa gctgttaacc tatgataatc agacgattat
     4021 tcttccaaac ggcaatctgg caaataacac cattgtgaat gtcactgctg ccgaatccag
     4081 gcgatgtgat gtgaaggtgt ctattgctta cagcgcagat attaagaagg caaaggaagt
     4141 tctgacaaag gttctctctg aagatcccga tactataaag gaaagagagc attttgtatt
     4201 tgtagatgaa ctggcagact ccggaatcaa cctatgtgta cgctgttggt tcaaaaatga
     4261 ggatttctgg cagggaaaat ggcgtattac cgaacagtgt aagtatgcac ttgataaagc
     4321 agaaattgaa ataccgttcc cgcagatgga cgtgcatatg cgttaattaa aaatagatat
     4381 tgaattttac agactataat gataatatta ccgttgtgat tcaatgtttt aatatttaca
     4441 tttttaggag gaaaaaatta tgaaaaagtt tctggcactt gcacttgcat ctgtcatggc
     4501 tgtttctctg gtagcatgcg gcagcaccaa tgctgacacc aatgcagaat ccagtgcggc
     4561 agctaccacc actgcaacaa ctgcagagag cagcagtgaa gaacccggtc tgaagatcgc
     4621 tatcgtatcc agcccctccg gtgtggatga cggttccttc aatcaggaca actacaacgg
     4681 tgttctgaag ttcattgagg agaatccgaa tgcaaccgta acccccatcc gtgaggagac
     4741 cggtgacagc gcagcagctg ttcaggctgt agctgatgtt gtagctgatt atgatgttat
     4801 tgtatgctgt ggtttccagt ttgcaggtat cggcaatctg gcacaggaga atccggatac
     4861 caagttcatc ctgatcgatt cctggcctac cgttgatggt accgaagtaa ccgcagatca
     4921 ggtgatcgac aacgtatacg ctgcttacta tgctgagcag gagagcggct tctatgcagg
     4981 tatggctgca gctctgtcta ccaccacagg taaggtagca gtagtaaacg gtattgcata
     5041 tccttccaac gtgaactacc agtatggctt cgagtgcggt gttaagtatg tgaacggtac
     5101 cgaaggcatg aacgtagaag tagtagagct tccttcttac gcaggtaccg atgttaccgg
     5161 cgctaa
//
LOCUS       DAWDHD010000182         5163 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_919_length_5163_cov_11.424041, whole genome shotgun sequence.
ACCESSION   DAWDHD010000182 DAWDHD010000000
VERSION     DAWDHD010000182.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5163)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5163)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5163
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_919_length_5163_cov_11.424041"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1195 a         1347 c         1065 g         1556 t
ORIGIN      
        1 tttgagaaaa agttaatgaa cacttgtact attttaatat catccgccca tgcatagttc
       61 ggcggataca cttccatccg cacagtaatc tcatgctgtt ttgtcagaat tgccgctgac
      121 tggatataat ttttcaccag cgccgtaagg tcgaaccgct ccatggccac cacatcatta
      181 ccaaattcca gctggttcag ggtcagaagc tttttcacca tgttgttcat cttcgctgct
      241 tcgtccacga tgacatcaca atagaactgc ctgctctcgg gatcgtcatt gatctcctcc
      301 tgcagccctt ccgcataacc ctgaatcagc gcaatggggg tcttgagttc gtgggataca
      361 ttggccagaa attctttgcg catttcgtca atggcttctt ttttctccac gtcccgttgc
      421 aattcattgt tcgctgtctt cagttccgaa atggttttct ccagggaatc ggacaactta
      481 ttgatattct ctcccaacag atcgatctca ttatgtgcct tcccctgata ttttgcctca
      541 aaatccagct gtaccatccg ttccgaaata ttattcagtt ccaggatcgg cttggtcaca
      601 ctccgggaga cgaaccagat aataatacca cccgccattg ctcccagaca tcctgcaagg
      661 gcatagaagc ggttggcgat ctttgcactc tcctgaatac tggaaagagg tgtctgcata
      721 ataaaagaaa tgccggtatt cagtcttccg tagatttcca gatattcctt gtcctcctgt
      781 ccggttctct tgatcacata atcatcgccg ttctcgatca ccctctggtc attgaaaggt
      841 atgctgagtc caaatacata accgatcagg cggttttcca gtctctcacc gccgttgatg
      901 gacacatatt tcatattgga attcacatcc ataacgcata ccgtcatgtt gtagcttctg
      961 cacacatcat ccagttctct ggcaaattct tccgtgtcat agctgtcgct ctccgctgcc
     1021 tgcttgatgg ttccgtaagc atcatgaata acatccagtt tgctctgcat atagactttt
     1081 cccagaaata aatagttgat catcaggcac agcaggatcg taccgatcat caggccgata
     1141 aagatccagg caaactgtcc cctgatagaa tgtacttttc tcttttgttc tttattccgc
     1201 tgtttcatcc atcttgtacc ccatgcccca tatggtcttt atgagattgc cttcctcgcc
     1261 cagtttgctg cgcagttttt tcacatgtgt gtctatggta cgggcatccc caaaataatc
     1321 atagttccac acattgttca ggatcttttc tctggacagg gcaatgcctt tattttccat
     1381 aaaataggtc aggagttcaa attccttgta acttagatcc acatcttttc catcaatgat
     1441 cacgcggtgt gccgccttat ccacggtaat gccgccgcag gtcagagcct cttcctgtcc
     1501 cagctgattt gttctgcgca ggattgcttc cactcgggcc accagaatct tgggactgaa
     1561 gggcttagaa atatactcgt ccactcccag ctgaaatccc tgcagttcgt ctctctcatc
     1621 tccgcgggct gtcagcatga tgatcggtac tttggagttc acccggatct cccggcacac
     1681 ctgccagccg tccatcttcg gcatcatcac atccagaatg atcagtgcaa tatctttttc
     1741 tttataaaaa atatccagtg cttcctcacc gtcaccggct tgcagcacat caaaattgct
     1801 ctttaccagg aaatcccgga ccagcttacg cattctgctc tcatcatcta ctaccagtac
     1861 tttcagtctg tccatacacc ctcaccctcc tatcttgaga tagtaactat tcagccccat
     1921 tcgcaaaatc gcctcttcga ggctttcctg aatagtaaca ctatagcgtg taaatatgtc
     1981 aattttgtga aacttcggtc tctatatgta attctctttc cttttcccgt accgtctgct
     2041 ccctctgggt cagtttctgc tcccaggtgg catatttgtt ctgcagattt ctttcataat
     2101 tcaggacatt gggctgatcg gaattcagcg tgatgataaa cattgcaata attgcaatga
     2161 ccatcacaat attcataata agagacagcc ggaacgtcgt atttttattc tcttccggtg
     2221 ccggtttgat tcttcttctg gcaggagacg ttttttccct ggtgtccgca aagttcgtcc
     2281 acagagggat aggcggaatc tcttctccca acagatcctg ctctgctttc agttctccct
     2341 gcaattccct gaggtattcc aaacctaccg gagtgcggaa caccctgtca tccaatgctt
     2401 ttttataaat aaccagtacg gactccagat ttgtccgatc gatccgctta tccagataag
     2461 caatcttctg tctctcctgc tctgccagtc tggcatcttt ctctgaatag aatgcaaatc
     2521 cggacactac atgatccgtc cggttttgct gctcctgttc tttctgtaca tccattactg
     2581 gttttcctcc gcgcttcctg ctgcagcatt ctgcaataaa atatgttctc cctgtaccag
     2641 cgggtaatgg acaggacgat cactgtccag attctcccgg tacatatcta ctgcagtaat
     2701 ctgatgattt tcataggtcg tataatagta aattcctctg tctgcattgc agcaggaagt
     2761 gtagatagtg atctcatact cccctttgtc cagctgacag catcctctgg tctgatccac
     2821 gctccccaga atatggaaaa actggctgac gctctccgac tcagacaatc cggacaggga
     2881 attcatcttc gtgaatgcca cccgtacaaa acgggatgca gaagacagat ccccgggcag
     2941 tcccatggct cccatgcctc tactgtacgg cttcagcttc agatctgcgg aaaaacggtt
     3001 tgtcggtgat tcggaagaca gggacatata atcgttcaaa cgaaacatct ggattggaaa
     3061 cgggggattg ttcgtcaata ctcccaccag gttgtcatag atcttcagtc cctcctgcac
     3121 agactcgatt gtaatggcct cttccttgtc tgccagaatc cagtgcagtg gagaaaccgg
     3181 atatttttca ttaaagggcg tatccaccag attgatatgg gataacaata ctctggcttc
     3241 ctgcacattg gcacactgcc ccagaatcca ggggatgaac tcaaactggg cgatgttgtc
     3301 cttacctgtc tgcactttgc tgtatactgc atttcccggg aaattcagtc ccgccatgcc
     3361 gagtcctttc tcattcactg catcatagta caaaggataa tcccctgcca tatgagcaat
     3421 gccgatcatg gcataatgct ccgtcagggc attctgatgc cgtagcggaa aaggataatt
     3481 gcgtggtgtt accgttacct cttccccata gttaaattca taatcaaaag ttctgccgaa
     3541 ataaaaatcc tgtgatctat aagttgccgc tgtacacata acatatactc ctttattctg
     3601 attcaaatag atatagaaac tagtcttagt ctatagcatt tgcagaatgt ttgcaagtat
     3661 acgaaaactg ttgacgcaaa tcatattctt atctttacta agcaaataat aaggagcttc
     3721 gaggctgtta tcagtatctc gaaggtcctt ttctgctata tctaattcac ttgccgatta
     3781 aggattacac cgcttacagg gcatatatcc catattgatg atatcttctc tgttgccggt
     3841 atatatctct ttgtttttct ccttcatctg atccacgctg gagcaggacg gatagtggaa
     3901 tttcatggtg ttggtattca gcacataggt agtttcctgt gattcctgtg gcactgacgg
     3961 ttccggtgtg atctgcaccg tagtgtctgt ctggggttgt tgtgtcggtg tttctatcac
     4021 aatgggtgct gccgcttcct gggatgccgc gttgctctct gcccagttat ccccggtagc
     4081 ataatcaata ccgatccccg gctgcacatt gtaggcaaat acacagaatt ggattccctc
     4141 tccggcatct tctacggaat acgcttccat gaggactcca tctgccagaa ggttatcacc
     4201 gtcaaaaata ggtgtgacac ggtacaggac atgattccct gtatttttga tataatcgca
     4261 gactttcgtc tcaaagggaa gcattccctg tgtattcaga tatctggttc cggtgatcag
     4321 gttctgcggg ttggcgtttt ccgctgccag catgtaagcg atcaggtggc agcgattgta
     4381 taggtatttt ccgtccacgt tatcgtattt caccagatgc cagccggtag gcttcacagc
     4441 tccgatctcc cctctcggtt ctgtcggcat cagatcctgt cccacgttgg catatgccac
     4501 gccacagcgc cccaggctgt ccagctcgct gtatgtctcg aaggactgtg tagtcagatc
     4561 tgcttctgtg aaataaggtt tgctgccatt gacttctgta taaggtgtgc cggagtatgc
     4621 cggaatctcc cggagggaaa atgctgacgt gccctctcct gtcgaagcat ctgttgcaga
     4681 tgtgacctgc tgcgattcct gtgtatccga agcgttttcc tgtggctgtc cggattcagg
     4741 ttccgatgct gcagtctccg atgcctccag caattcgtag gggatcgtcg ccggctgctc
     4801 tatcgtatag gtattatcct gcggcgtgac ggtgctttcc gcacagccgg taaatgtcaa
     4861 tagcagtacc agccatagtg acaattgtgt tatcaaacgt ttctgaaatc tgttatgttt
     4921 cattttcata gcttgtcaat tactcccttg taaaactctt tggaaattat accacatttt
     4981 tcttcatccg acccgtgaat tatactgagt tttgttgact ttttcagcag atttgggtat
     5041 attacagtca aaagagatag ccttccctga agatcgttcc ggatgtttgg ttgaggcaac
     5101 atgaaatgca atcgagtagg aggcggtgat taaccgccgt cctctcacac caccgtgcgt
     5161 acc
//
LOCUS       DAWDHD010000183         5144 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_926_length_5144_cov_9.295343, whole genome shotgun sequence.
ACCESSION   DAWDHD010000183 DAWDHD010000000
VERSION     DAWDHD010000183.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5144)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5144)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5144
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_926_length_5144_cov_9.295343"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1138 a         1373 c         1074 g         1559 t
ORIGIN      
        1 acctgtttat tccgttcaca ttctcctaat tatgacaaat tctcatctct gttcatcaga
       61 cgtaccagct gcctgtgttc cagctttttt accgactgtt ttgccggcag taccgtcttc
      121 agcgcctgga tcatggtaca gccgtaattc cggcggatcc aggcagccag agcaatcatt
      181 ttgtcctcca ctccggtctc tttttccggc attcctgcga tatccttgat cttctccgga
      241 gcgaactgac acgtttcccc caaagccacc acgtaaccct tgatgagacg attgccgttg
      301 ccaaacggga tcatgacaca ggctcccggc tccagtctgc cccgcagact ctccggcacc
      361 cggtattgaa aggttctatc cagtttttcg tgggagatat ctacaatgat atccgcatat
      421 aacgtattca atctgttttc ctcattattt tgctgcagat cccggaatat gtccttccgc
      481 cagaatatcc cgaatcagat ctctctcatc catgggatgc ttcaccccgc ggatctcctg
      541 gtaatcttca tgtcccttgc ctgccagcac gataatatct ccgggttctc cgtgttcgat
      601 agcataagtg atggcttcct tacgatcgca gatctctaca tagtttccat ccgtttttgc
      661 cataccggtc ttgatatcgt cgataatatc ctgcggttcc tcaaaacggg gattgtcgga
      721 ggtaatgatg gtcagatccg ccagcttgcc gctgacctct cccatctcaa aacgacgctg
      781 tctcgaacgg ttgcctccgc agccgaacag gctgatcagt ctgtgaggtt catattcccg
      841 caacgtggtc agcagactct ccagcgccat ggcattgtgg gcataatcga tcagcagggt
      901 gaactgatcg gataccttga tcatctcgat acggcctttt acctttgcct gcagcagcgc
      961 ctttttaatg ttttcctcat ctaccttgaa atgacggcag atggcaatag ccgccagcgc
     1021 attgtacacg ctgaatttgc cgggcatggg cacttccaca tcaaaatcca tcagtccggc
     1081 tacgtggaaa gtcacgccca gttctccggg cttatgcacc agtttccgtt cttccgcccg
     1141 gagcatacag tgctctccca ttccgaagtt ctccagtgtg caggtatgtc cttccgtcac
     1201 tgcctgccag tgctcatcgt caccattcac aatacctacc ttgcactgtt tgaacaataa
     1261 tcccttgcag tgcagatatt cctcgaagct ggcatgctcg ttgggaccga tatgatccgg
     1321 ttccagattc gtgaaaatac cgtaatcgaa cacaaagccc tgcgtacggt gcagcatcag
     1381 cgcctgagag gatacttcca tcacaaccgt atccagtcct gcctccacca tacgggcaaa
     1441 atactcctgc agcagatagg attccggtgt ggtattgttg gcatggatat gttcgtctcc
     1501 gatgatcact tcgatggttc ccaccagtcc cactttatag ccggcatttt ccagaatgga
     1561 ctttaccaga taggtggtcg ttgtcttacc tttggtaccc gtgataccga tgactttcag
     1621 cttttctgcc ggatggccaa accaggccgc agaaataaat gccatggcat atctggtatc
     1681 cgctactttg accaccgtca catccacatt ctccggcagt tccacgtctt tttgcaccac
     1741 tagtactccg gctccctgct ctgccacctg tgcagcaaaa tcatgaccgt caaaatttgc
     1801 tccgccgatg cagagaaata agcatcccgg aacgaccttg cgggaatcat agaccaccgc
     1861 agggatttct ctgtccagtg ttccctgtac cagttcatat tccaagtgct ccagaagctc
     1921 tgttaatttc ttgttttcca tcttattctt atccttttcc atagaggtat ccgcctttct
     1981 ctcaggaatc tttttcacat actgtatttt attttactct tttttctcag aatgtaccac
     2041 ccttaaattt agttgcatct tttgcttttt ccgaagcaat ggtataatat tactgatttt
     2101 tatttaaggg ggaattctca tgaacggttt ctatggatct gcattcggaa atatccttgg
     2161 cacactttat tttcttctat ttcaatgtgt ggggtttctg tacataaaac gtatttttcc
     2221 taaaaaggga cttctgttcc atctgctgac cggcagtgta tccggctctg ttctgttgca
     2281 gtggattcct gctctgtttg ccttcttctt cggtttttcc atgacagctc atattatggc
     2341 tctgcttttc gtagtcattt gtctggtcct gctatatgtc ttttaccatc ctgccacggc
     2401 agtatcttca actgctccca ccaaagacta ccggcagctg ttgcgcagaa attatgcctt
     2461 tcttctgctg gcaggcggta cctttgttct gttttgtatt ttgttatcca cccacaccat
     2521 cctgccgaag gacgacggtc ttcatgtggg gcagtgtact tatggggatc tgcagatgca
     2581 cctgggaatc attacaagta ttgccaatca acagaccttc cctccttact actccatctc
     2641 tccctgggat aggctctgct atcctttcct gtgcgacagt atttcttcca gtatttacct
     2701 gtttggtgca tccctgcgct atgcttatat gctccctatg tattttgcct ttttccaggt
     2761 cataacggga ttttatgcca ttgccgatgt tctgttccat gatcgcgcca aatctctggc
     2821 tgcctgggtc ttatttttct ataacggcgg tcttggcttc gtatatttca ttgactggag
     2881 cagagaaggc ggttataaat tcagtgatat ttttaccggc tattacacta cccccaccaa
     2941 tctggtcgac cggaatatcc gctgggtcaa tatcatcgca gatatgctcc tgccacagcg
     3001 tgcaacactc tttggctatg cggtcctgtt ctgtgccatc tggctgctgc ttcgggccat
     3061 ccggaacggg gaaaaagaat gttttctccc tgccggcatc ctggcagggg cattacctat
     3121 gatccatacc cattccttcg ttgccatcct gatcttaagt gcctgttgga tgttgctgtg
     3181 cctttaccgg tccgtgccgc ataatacttc cccggtagcg catccgggag ccgttctgct
     3241 cggatgtttc gtgacctgta tgattcttct ggaaattctg aacgaaagtt ctgctgccgt
     3301 tgcacctgta ctgctctttc gttttggcat ccttgttgcg gcatccctcg tgctatacgg
     3361 tctgtcctta ctgtaccggt gcttttccgg taaaagtaca aacaatgata cactgcagaa
     3421 gtttttgacc acctggggag ttttctttgg tgtgcttctg cttcttgcac ttccgcagtt
     3481 attggaatgg acttttggcc agacaacgca atccggcttt ttgctggggc attttaactg
     3541 gggaaatcag ggcgatactt atttgtggtt ttatctgaaa aactggggtg cgatcctgct
     3601 atggttcgtc cctgccatga ttcattgtaa aaaagaagat tttgatgtga tgtccggggc
     3661 atttctcctg tggtttgttg tggaaatcat ttccttcacc cctaacacat atgacaataa
     3721 taaactatta tatataactt atatatttgt ctgctgcctc agtgccgatt acggaatcga
     3781 tttcctgcgt tctctgaaaa aagcctcttc acgatacctc tgcatgatcg gcatggtatt
     3841 tctttgcacc ttttctgccg ttctgaccct atgcagagag gtaatatccg attataccgc
     3901 ttattcttcc gcacaggtgg aagctgcagc attcgtcgag caaaatacct ctcccacatc
     3961 ccgttttctt accaacaaca ggcatgtaaa tgaaattgcc gcccttgcag ggcgcaatgt
     4021 gttaaacggg gcgggaacct ttttggcaat gcacggtctc tatcacacag aatatcacga
     4081 agatttccgg acaatttatg agactcccgc tgtttctgct gatctgatcc gtacttacga
     4141 catcgattat attgaagtct cttcctggga acgggcaagt tacgctgtcg atgaaaacta
     4201 cttccgctcc gcctggccct gtatctttga caacggagaa attcagattt accagatcaa
     4261 tccataattt cacacttgga agtcgcaaga ccctccgcat cactgttgga tgcacttccg
     4321 aagttctgga tcaggccgat cagctgtccg gacataagca cggtaaacaa cgaatatccg
     4381 atacgtctca ccggaatgta gcttaaggac attaccagta cgatcagatc cgacatcaga
     4441 tacacccact ggatcttcca gccggtagca tgggaaatac tcatggccag ggcatcatct
     4501 ccgctggggg ctccgccgat gcgcacgcac agtccggcac cgacaccgac gaacaatgct
     4561 cccaccagtg ctgccagaag gggcatattt gccagttccg gccacagatg aggaaattgc
     4621 tcgcagattt tataggttcc tgagaatcct gcggtagcca gagccgaata ggcgataaat
     4681 tccttgccta acagcttcca tcccaggata taacataaaa tattcataat ccctccggta
     4741 aacgccggag agattcctgt ccaatgctcc aacagcaacg tggctcccag cacaccaccc
     4801 tccgttactc cggagatgga gtgcacgtta tataatccaa atgccagaat aaaacttccc
     4861 aacaaagtta tggcacaatt ttttaaggta atatttttca gtgcctgtct ccattcgtaa
     4921 atctgtgttt tcatttttgt gctgttattc cttctatatt tttataattt ttatccctat
     4981 ttttatatta taatccataa ctttcgggtg gtgtcaagca ttatcagaaa ctattttgct
     5041 tttccagaca tcgtaaagcc ccaggctgtt gcgcctgagg ctttacgatt ttcttatgag
     5101 ggggggagat agtgagttca tcaactatct gaaatacata ttac
//
LOCUS       DAWDHD010000184         5139 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_927_length_5139_cov_10.229150, whole genome shotgun sequence.
ACCESSION   DAWDHD010000184 DAWDHD010000000
VERSION     DAWDHD010000184.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5139)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5139)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5139
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_927_length_5139_cov_10.229150"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1462 a         1121 c         1436 g         1120 t
ORIGIN      
        1 aaaaatgatc agagtaggaa tcatcggagc aaccggttat gccggaggag aactggtaag
       61 gatcctgtta aaccacaagg atgccaaaat cgtatggtac ggttcccgaa gctatataga
      121 taagaagtat tatcaggtat accagaacat gttccagctg gtggaggata tctgcaagga
      181 tgacaatctg gaagaactgg cgaagcaggt agatgtgatc tttaccgcaa cccctcaggg
      241 atttctggcg ggccttttga cagaggagat tctctctcag gtgaagatcg tggatctgtc
      301 tgctgattac cggatcaagg atgtggccac ctatgaaaaa tggtacggca tcgagcacaa
      361 atctcctcag ttcattgagg aagcagtata cggactctgc gagatcaatc gtgacaagat
      421 cacggagaag accagattgg tagccaaccc cggctgctac accacctgtt cgatcctgac
      481 ggcctatccc atggtcaagg aaggcatgat tgatgtgaac accctgatcg tggatgccaa
      541 gtccggtacc tccggtgcag gaagaggcgc caaggtgccc aacctgttct gcgaagtgaa
      601 cgagaacatg aaagcctacg gcgtggcaag ccacagacat acccccgaga tcgaggagca
      661 gttaggctat gcgggcaatt gccaggtgac cattaatttt accccgcacc tcgtacccat
      721 gaacagagga attctggcaa ccgagtatgc aacactgaag agaaaaccgg acggtacact
      781 tcctacctat gaggaagtaa aagctgtcta tgataaatat tatgcaaacg agaaattcgt
      841 ccgtgtgttg gataagggca gctgccccga gaccaagtgg gtggaaggca gcaactacgt
      901 ggacatcaat tttgtcattg atgagcgtac cggccgtatc attatgatgg gtgcactgga
      961 taacctcgta aagggtgccg caggacaggc ggtacagaac atgaacctgc tgttcgggct
     1021 tcctgagagc gaaggcctgg agctggttcc atgcttccct taaagcagat acggaaagga
     1081 ttagaaagga taaaacacat acatgaacaa acagtttacg atcaaaccca tgaccataga
     1141 agattatgac ggtctgcatg ccctctggat gaccattcac ggcttcggta tccggagcat
     1201 cgatgattcc agagaaggtg tggaacgttt tatcaaccgg aatcccggca ccagtgtggt
     1261 agccatcggg gaggatggag ccgtagtggg tggcatcctc tgcggccatg acggacgacg
     1321 aggctgtctg taccatgtat gtgtcagaga agattaccga agacttggca ttggcaaagc
     1381 catggtcgtc cactgcatga atgctctgaa agcggaacat atcaacaagg tcagcctgat
     1441 cgcttttacc cagaatgaca tcggaaacgc tttctggaaa tgcattggat ggacaaagcg
     1501 ggaagacttg aactactacg actttacttt gaatgaagag aacattacgg cgtttaataa
     1561 atagatgcca ggattgtgga agcgcgtagc acgaagcaat ccgtaggcat caaagtcggg
     1621 ggcttttgac ccagacacca taaataaaac ggaagcgaaa ggagattatt ccaacatgaa
     1681 acagatccag ggcggtgtga ccgcagcaaa aggctttgag gcagcaggtg tggaggctgc
     1741 cattaaatat cagaatcgta aggatatggc actgatcgta agtaaggcac cttgtaccgt
     1801 agctggtaca tttacctcca atgtggtaaa ggcggctcct gtactgtggg ataagcagat
     1861 cgtggaacat tctgcatttg cacaggcagt ggtagtcaat tcgggaatcg ccaatgcctg
     1921 caccggcaaa caggggctgg actgctgtga agcagaagca aaatgtgccg gagaactgtt
     1981 gggcgtgccc accgatgccg tactggttgc atccaccggt gtcatcggta tgcagattcc
     2041 cgtagacaag atcaccgctg gcatcgagaa actggtggca gccaaggcag atactctgga
     2101 ggctggctcc gatgcagccc atgccattat aaccaccgat accatttcca aagagatcgc
     2161 catcgagaca cagatcggtg gcaagactgt gactctgggc ggtatgtgca agggttccgg
     2221 tatgatccat ccgaatatgt gcacgatgtt aggttttgta accacagatg tgaaaatttc
     2281 caaggagatg ctgcagaagt gcgtcagcgc agatgtagtg gattccttca acatgatctc
     2341 cgtggatggc gatacttcca ccaatgatac actgctggtg ctggccaacg gtatggctgg
     2401 caacgaggag attaccgcag agggcgagga ttacgacaac ttctgcaagg cactgcatga
     2461 gatcaccaca ttcctggcaa agaaaatggc aggagatggc gaaggcgcca cagcactgtt
     2521 cgagaccaaa gtaataaatg cagtcagcaa ggaagatgcc agaactctgg caaaatccgt
     2581 gatctgttct tctctgacca aggcagctat cttcggacat gatgccaact ggggacgtat
     2641 cctctgtgca ctgggttatt ccggagccaa attcgacccg gaaaatgtgg atctgtattt
     2701 tgagagcaag agcggcaaga ttcatatctt cggtaatggt gtggcatgtg attacagcga
     2761 ggaagaggcc acgaagatcc tctccgatcc ggaagtcacc gcactggtgg atatgcatat
     2821 gggtgaggca gaagctaccg catggggctg tgatttaagc tatgattatg tgaagatcaa
     2881 tgcggactat cgttcctaaa aaaagaacga ccggaaagat aaatgacagg cggatcacgg
     2941 tatctgcgga aatgaggata aaaatggaaa aagatatgga acaggtaatg aaaaaagcag
     3001 aggtgctgat cgaggcgctt ccttacatac agaaattcaa ccgtaagatc atcgtggtaa
     3061 aatacggcgg cagcgccatg agcaatgagg aactgcagaa aaacgtcatc aaggacgtaa
     3121 cgttgttgaa actggtaggt ttcaagccca tcatcgttca cggcggcggt aaggagatca
     3181 gccgctgggt cggcaaggtc ggaaaagagg caaagttcgt aaacggtctg cgtgtcaccg
     3241 atgaggagac catggagatc gcggaaatgg tactgaacaa agtaaataag agccttgtga
     3301 ccatggtgga agagctgggc gtgaaagcag taggtgtcag cggtaaagac ggcggcctgc
     3361 ttcaggtaga gaaaaaatat tccaacggtc aggacatcgg ttatgtgggt gagatcaccg
     3421 gcgtgaatcc ggatattctc tttgacatgc tggagaagga ttacctgccc atcatcgctc
     3481 ccattggact ggacaaggat tatcacacct acaatatcaa tgcggatgat gcggcctgcg
     3541 ctatcgcaaa agcagtacat gcggagaaac tggcatttct gacggatatc gagggattgt
     3601 acaaggacat cagtgacaag tccacattta tttccagatt gacagcttcc gaggcagagg
     3661 gactgatcca cgacggttac atcggcggtg gtatgctgcc gaagctgaac aactgtactt
     3721 ctgcgatccg caacggtgta aaccgtgtgc atattctgga tggacgtctg gcacactgcc
     3781 tgttgctgga gatcttcacc aatcagggta tcggaactgc gatcgtatcc gatgaggagg
     3841 aaaaataaaa tgagcgacac catgaaagaa tatattgcag aggcggaaaa agacctgctg
     3901 catacctata accggtatca gatcgttctg gataagggcg aaggcgtaca tctgtatgac
     3961 accgatggga aaaaatatct ggatttcgta gcgggaatcg ctgtgtttgc cctgggctat
     4021 cagaacaagg catacaacga tgcgctgaaa gcacagatcg acaaggtgat ccacacctcc
     4081 aattattatt acaacgtgcc tgccatcgag gcggctcgta agatcaaaaa agtatccggc
     4141 atggatcgtg tctttttcac caattccggt gcggaagcag tggaaggtgc cattaaggct
     4201 gccagaaaat atgcgtatct gaaggatggc tgtaccgacc atgagatcat tgccatgaat
     4261 cactccttcc acggcagaac catgggagct ttgtcagtaa cgggcaatcc caagtatcgt
     4321 gaggcgttcc agcccatgat cggacatatc aaatttgcag aactgaatga tttccagagt
     4381 gtgcgggatc aggtgacaga caagacctgt gccgtcatta tggagactgt acagggcgag
     4441 ggcggactgc gtcccgctaa tgaagagttc ttaaaacagg ttcgtgatct gtgcgatgag
     4501 cgggatatcc tgctgattct ggatgagatc cagtgcggca tgggacgtac cggttacatg
     4561 tatgcatggc agcgtttcgg cgtgaagccg gacatcatga cttctgccaa ggcactgggc
     4621 tgcggtgtac cggtaggtgc atttatgatg acggagaaag tagcacagca ttctctgaca
     4681 gcgggagatc acggaaccac ctacggcggt aaccccttag cctgcgcagc agtggaaaaa
     4741 gtgcttggat tattcgaaga ggaccatata atagaacatg tgagagaggt ggctccctat
     4801 ctggaacagc gtctgaacga actggcagac aagtatgact gtatcaaaga acgccgtggt
     4861 gtgggactga tgcagggact ggtatttgac catccggtag gagacattat taacggagca
     4921 ttggagaagg gattaattct gatcaatgcc ggtgcggaca ttatccgctt cgtaccgcct
     4981 ctcgtgatta cccgggaaaa tgtggacgaa atgatcgcta ttttggacac ctgtatcgaa
     5041 taagaggttt aggtgaattt atgtttttga aaaaaagaat tatcgctaca gcagctgtga
     5101 tcgccatggc tgctgcagct gtttacggaa gtacgcagg
//
LOCUS       DAWDHD010000185         5075 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_945_length_5075_cov_7.860558, whole genome shotgun sequence.
ACCESSION   DAWDHD010000185 DAWDHD010000000
VERSION     DAWDHD010000185.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5075)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5075)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5075
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_945_length_5075_cov_7.860558"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1321 a         1284 c         1168 g         1302 t
ORIGIN      
        1 ctctagcagt ataaaatggc aggctcatct ttgcaagccc gctggcattt atttttttct
       61 gatacatccg ccttatcttc tggccggcat ctaagaaagc aaaagaaaac catttctgct
      121 tctgcttttc ggcagtattt tccggggcag cctccaggtg ttcccgcttg tccatgctct
      181 ccggctgttc agaaacagtg ccggggaggg aaaactgcat tctgtccctg agaaatgtga
      241 tcagctttcg aattccgcgg tacagcagaa atacgcagac gatcagaagt gcgatcatgg
      301 ccacatattc cagaatgacc cataaaataa aaggatcttc cgcttccatg ggaggcatat
      361 cgccaccgcc tgcctgctgt tccaccacta ccgcttctgt cccggaatcc atggggagca
      421 gtccgaacag gaaacgaaga atcgcagcca gaccgtttcg cagtaaccgc aacaagggct
      481 ttaaccaggc aaactgtgac actgccagga gcagaagccc cgacaacagc acaaaggctg
      541 cggtgctgtg gagcccggaa cgaaaaatct cctgaaacgg gatcttgccg gtactcagcc
      601 tgtttaccgt aaggaaatcc tcataggact gcaaaaacaa aatgacagca tacaataaaa
      661 acaccaggat cagactcata cgataataga gatcccagac gttgcttccc tgataatgct
      721 gtagaaaaag acacaaaaag gcaattccgg cggctaacgg cagaggcagc ggatcggaag
      781 caaagtcctc cgtccgaaaa cgcagccaga gggaatagat cacaaagcct gcacctacca
      841 acagccggat accacggttt acgccgttgg ctgccggaat gatataaaaa catgccagta
      901 ccagtccgtg cagaaggagc aataccgaca gatgcttaca gcgttttctg gcgtaataca
      961 gaaaaaacac cggcagcgct ccgagcatcc acaaagccaa ggacggacgg ctcgccggag
     1021 ccagcgcggc caacaacgtg gacgcagtca gcgccagggg ataaaaacat aattgattca
     1081 ttagatcagt caaaatttct ttcacattca gacgctttct tcccgtaggg agataaaagt
     1141 gatcaaagac tttaacgctt ccccgggatc aggaggtgta ctttcggaat aaggataaaa
     1201 ccatttcata tccgggtgtt tttcctgata ttgcagaagt aatgctgcaa agtcttcata
     1261 ggcattgggt gatacaaagc aggtatagag cgcattggac gcatcggaca tacgggcggc
     1321 aaacagctcg gaaaaagact gtaccggttt agccgtatcg attcttgcaa gcgtctgcag
     1381 acagacattc cgctgtctga ctccgccgcc ggcttccaga atacaggggt ctccactgat
     1441 tgtgtcaata ccgttgcaat acagggcaac ctgcatcccc tgttccagaa acagcaataa
     1501 cagtgccgtg gcaatctgca ggcaggcttc cacgcagtcc tcttttttca gaataccggt
     1561 gtcctctaag ttcacaaaaa tccgaacgga tttctgggag gtataatttt tctggtttac
     1621 cttcagatcc ccggtacggg ccgtggcttt ccagttgatg ctttttaagt catcctgtgg
     1681 ttggtaatcc cgaatgcccc ggtattcgaa agggtcttcc aaaagatgtc gttttgtcag
     1741 aacttctccg tttaactgtt ttaaagaccg tttaaaatcc ggatgggaaa agggcttcgg
     1801 gtacacatag agactgcagt gttcgatggg tgtcgtagcc gtatactgtg ctgtcaggaa
     1861 cagatcggac gcgacaagat ccgcctcctg tatggtgtaa taacctcttc ttccgccggt
     1921 aaagtgcagt gctctggtga ccttctccag acgtccgatc tggaaaatat cattgcggta
     1981 atagcgatcc gtggtccggg agcctttggt atcggcaaac attagatgtc tgtcagtctg
     2041 gaatttgacc ttcaacatgg taaggggcag ccgtttgcga ttctcaatga tctccgacag
     2101 ctcgctctcc tgtccttccc agatgccatc ctcccggaaa gaaagggaaa ccttcagatc
     2161 ccgctgccat attttggcgt atacatattt ctgccccaga tacagaagca gggcaaagac
     2221 aatgattccc agcagttgta tcatggctca aacctttctg tgggaacagg aacgttggaa
     2281 aggatgtttt ccattctgcc tgctgcggag ccggtatcgg aaccgaagct gcataccagt
     2341 ctgtgggcca gcaccggaac agccagggta cgaacgtcat ccgggatcac ataggaacgt
     2401 ccctgcagat aagcataggc cttgacacag cgtaaaagcc caagggttcc tctgggactg
     2461 acgcctgcca gaataccgtc tgcccgtctg gtggcttctg caatctgtac catatattcc
     2521 cggatgcagg gatgaacaaa aatcttcgca gcctctgccc gggcagccag aagttcctcc
     2581 agagagacca cggcagtcag gcttttcagg ggatcttcct ccatgtacaa ttccagaatc
     2641 ctcagttcct cttctttggt gggaaggttc atggacagtt ttataaggaa acggtctaac
     2701 tgcgcttccg gcagaggaaa tacgccggca ctttcgatgg gattctctgt ggcaatgaca
     2761 aagaagggtt cgcccggtgt ataagagacc ccctcgatgg ttacctggcg ctcttccata
     2821 cattccaaca gtcctgcctg ggttctgggg gtggcacggt tcaattcgtc tgccagtaaa
     2881 atattggtaa ataccggccc ctttcgcagg gtgaattcat tcttctgtct gttatagaca
     2941 ttgatgccgg taatgtcacc gggcaacaga tccggggtaa actgcacccg ggaaaactgg
     3001 gcatttaaag attttgccag tgccttggcc agcttcgttt tgccggtgcc ggggacatcc
     3061 tctaatagta catgtccgtc agcgattaag gccgtcagaa gaagttcggt cacttcctct
     3121 tttccgacca ggactttttg aatattgttt tgtattctgg atgctaattc ttttccgttt
     3181 tgttcagcca taatttctcc taaattacta aaactattca gaacttcatt cgcaaaaaac
     3241 tctttcagag ttaccttttt gcgaatgaag tgcttacatg gctctgaata gttatcatta
     3301 taccttataa aattaagttc tgccagtacc aaaaggcaca taaatatgaa ctgcgaagat
     3361 tgcagatacc agaaaggaat gtaaaagagc atggaaaaac atgatcacag acataaggaa
     3421 gaactcttac agcagatgcg cagacaggca gaaatacggc cagagcaaaa cgattcggaa
     3481 cctgccgcat cttccgatac cggtacgttc aaaatccgcc ttgctctggc cggagtttta
     3541 ttattgacga tcatattgct ggatcaaaaa ggcaaatgct ttgccgggat ctccatggat
     3601 caggtgctgc attatgtcac cgatgtagat tacggcacct atctggagac ctgggtggaa
     3661 actgcagtta acggttcgca agctctgtag tgatctcttc ccaggtgaca tccttttccg
     3721 ccatcagcac catcatgtga taaaggaaat cgctgatctc gtatttgacc tcgttggggt
     3781 taggattctt ggcagcgatc acgatctctg tagcttcctc tcccagtttc ttaaggatct
     3841 tgtcaatgcc cttgtcgaac aggtaattgg tataggaccc ctccttgggg tggaccttgc
     3901 ggtccttgat cacatcgaag acttcctcga atacctgtaa gggattgttg ctttcctcat
     3961 aatccttttt cgtgatctcg ttgaagaagc agcttctgct tccggtatgg caggcagcgc
     4021 ccacttgaga taccttcgca aggattgtat ccatatcaca gtccgccacc agttccttca
     4081 catactggta atgaccgctg gtcagacctt tcagccataa ttcctgacgg cttctgctgt
     4141 agtaggtcat cttgcccagc ttcagggtct gctcataggc ttcctcattc atataagcca
     4201 tcatcagaac ttcctgtgtc cggtaatcct gtacgataac gggtaccatg ccgtcactgt
     4261 tctttttgaa atcttcccac ttgtaggcag cctggaagga ttcaatggga atatcgttct
     4321 cccggcacag atccttcagg gctacgatct ctttcacatt gtcatttatc gtattgccgg
     4381 tgacaccaca gacattctca taagcaaaaa tctccagcaa tttattcaat gccacctgat
     4441 tgatctgcac gaaaaacgga agggaaagaa tggcctgagt ctcccggatc tgatgagggt
     4501 tcatcaggat catggcagag atatatttct caatcttctc cttatgtaat tccagaacgg
     4561 acgggtcgtt gtaagagatc agaatcttgt ccttaccgaa tttcagagag acctcttcgg
     4621 taatctcaat attactctct ttctcatagt cgagaactgc ttttttacat ccggcataaa
     4681 gtaatttctt aatgtcctcc atacgcttga cattgccggc accgatgaca tccacttccg
     4741 ccctggtaca gatctccttg atgatatcca gtgcggcatc atgctccgca tcaccttcgg
     4801 acatatcgaa aacgatcagc tcatccgcat tgtgctcaca gtaataatct gccagacgga
     4861 cgggatccgt atccacgatg gtggtatccg taagattccg caccgcatgc tcatgatata
     4921 aataaataca gggaacaaat ttcttgatga tcatagtaat cctcacgtct ttctatacct
     4981 gcaaagccta tgccgcaggc acgtattttt tacagacttc ctttggtact taaaacatcc
     5041 gttaccttcg gattatagga aacggcctga tccag
//
LOCUS       DAWDHD010000186         4981 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_980_length_4981_cov_8.045473, whole genome shotgun sequence.
ACCESSION   DAWDHD010000186 DAWDHD010000000
VERSION     DAWDHD010000186.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4981)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4981)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4981
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_980_length_4981_cov_8.045473"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1044 a         1580 c         1030 g         1327 t
ORIGIN      
        1 gccgcataca gcctcgactt ctgataattt ccatgggtga taacatcccc caccagtacc
       61 agcgctgtct taatgatgcc gtgttcttcc gctacctgtg ccagtgtctc caccgtacag
      121 atataagcct cttcctccgg ccaggttgcc ttgtagatca gggctgcggg ggtgttctcc
      181 gcataaccgc ccgccatcag tctgcggctc agttccctca gcatccccgt gcttaaatag
      241 atcgccatgg aagcctgatg ggctgccaga ctttcgatgc tctccttctg cggcactccg
      301 gtccgtcctt ccatacgggt gatgatcagg gtctgggaga caccgggcag ggtatattcc
      361 agatttaacg atgccgctgc gccgaagcag gcactgactc ccggacagct ctcataaggg
      421 atatgtaatg catccagtgc atccatctgc tcccgcaccg caccatagat gcttggttct
      481 ccggtgtgca gacgcaccag catcttcccc tgttcctgtg cctcctgcat aatctgcagc
      541 acttcctcca gggtcagttt tgcgctgtta tggatttcac agtctgtttt ggcatattgt
      601 aacagctccg ggttcaccag tgatcccgca tagatgatga catccgcctg ttgcagcatg
      661 tgcatgcccc gcactgtgat cagatctgct gccccgcttc ctgcgccaac aaagtaaacc
      721 attcctccgc ctccttactt ttcgccaatg ctccgaaatc attggcatac atgatacatt
      781 cgatcgcaag ctttccctgc gcccgctttt ccaggtaaaa taaaatacgc tccatggcat
      841 aatccataac ttcctgtctc ctgccgcact cgtccagaat ccgcaccgct tcctctgtgg
      901 tgacacagga caggagtctg gcagcatcca ctgccgctac accgcatttt acagcaaaag
      961 cggtcaacag ctccatcctg cagtcgccct cccgggaatg ggtattcata atacctcccg
     1021 ccactttgat gagcttcccg atatgtcccg caagaagcag acgcctaaat ccctcttcca
     1081 ccgccatatc gatcgtcaga ccgataaaat tactgcattt tacactttta tccagatcat
     1141 agtgataggt atttttcaga aaatccagtc cgtaattgcc cggagagatc accacatctt
     1201 ctctccccag tgcccgctgt tgccgcagtt ccacgcggat cgtatccaga atagcctgtg
     1261 tgctcatagg ctccacgaca ccgctggtgc ccagaataga aataccgccc acaatgccaa
     1321 gccttggatt aaatgtcttc tctgccaact tttcaccttc cggcacagag atctccaccg
     1381 acaggattcc acgataatcc agcacctggc acacctgtaa tacttcccgt tcgatcattt
     1441 ctctggggac gtgattgata gctgcatttc ccacgggctg atccagtccc ggcttcgtca
     1501 cccggccgac accgatgccg ccgtcgatca cgacctgtgc ctgttcaggt gcatgtccgg
     1561 ttccattctt cctaccgggt tcttctctgg ctttcctatc cgttatttcc gcaaaagaaa
     1621 ccttcgcaca gatcagcgcc cctgtggtaa tatcggggtc atccccgcca tccttttcca
     1681 cggcgcagct gacctcctgc tccttgcggc agatgttgcg gatcttggct gtgtaaggga
     1741 tacctttggg tgtctctatg gtgatttctg ttttctccct tccggtcagc agcatatagg
     1801 cggccgcctt ggccgctgcc gctgcgcagc ttcctgtggt aaatccgtac cgcatgtctt
     1861 ctacctcact gttactatat catgattcgc accttcggtg cttgacgttg cttcgcaact
     1921 atcatgatca aattcgccgt tcgccaatca tgcaagcatg ttggctctct tgcgctcatt
     1981 atatcgtgag cccagagcgc cagttgccga tcccgccggt attctggctc tcctgcgttc
     2041 cttatcctct gtctactccg tctcgccggt tgtcttgtgt acctttcaaa tcgttataca
     2101 attcattctt ttttcagttc atacagcaag gcattgcaaa tggccgctgc cacgttgctg
     2161 ccgccttttc ttccacgatt gatgatatag ggcacatccg tctctataat cagctccttg
     2221 gcagcttcca cattcacgaa acccacaggg accccgataa cgaatgccgg gcgccagtcc
     2281 ctttcctgca tcagctcata gatctgtatc aacgctgtag gcgcatttcc aacagcgaag
     2341 atcactggtt tttccagctt cgaagccaac tccatgctaa tagccgcccg ggtggtcttc
     2401 cgttccttcg ccatagccgc cacatccgca tccgccatat agcagtgggc ttccccgcca
     2461 aaagaagcca acgccttctt attgatcccc gccagtgcca tattggtatc cgtcacaata
     2521 tccgcaccgt tttttatcag gtttttagcg atctccaccg cgttttcgga ataggtcatc
     2581 gtctccgcat aatcaaaatc tgcgctggta tggatcaccc tgcgggtgac aggatcctgc
     2641 tccgcaggca aagtgatgcc ccgctctgcc agttctgcgg caataatttc aaagcttcgc
     2701 ttctcgattt cttccggcaa tacatattca atgctctttt tttccataag ttaccttccc
     2761 caaaaattca ttttccgtct tccagctgct cctatttact gctttgcaaa gaaaacgaac
     2821 tcattcagca gtaatctgcg caagttcctg taacagcact tcattctctt catgtttctt
     2881 caccgcgatc cgataatagc cctcacccag tccccggaaa ttctcacagt tccgaatcag
     2941 gatacctttt gcaagcaggc tgtcatagat gggctgtgtg gtatacagca acaggaaatc
     3001 cacttctccg gacaccaccc ggatgccaag cttttccagt ccttcccaca aatattctct
     3061 ctcgtttttc acatattcca ctgtgtcttg cagatattgc tctgactgct ccgcgcaggc
     3121 gatgcccgct ctctgggcaa acaccgacag gttccattcc ggtagctgcc tgcgtatttt
     3181 ctcccgaagc tctgcgttgc tgcataccag atagcccaga cgcacccctg gcatagcaaa
     3241 gcttttcgta aaggcccgtg ccagcagcag attttcatac tgtccgatct ccccaagcag
     3301 aaactttggc tgttcccggc cggactcgca gaactctata aaacattcat ccactactac
     3361 ggtgattccc tgctgcctgc aatgctccaa cagttcctgc agatattctc tgcccatcag
     3421 ctgccccgtg ggattgttgg gattcgctac aaacagcatg tctgtatccg ctgtcagctc
     3481 ctgtaacagc tcccgccccg gacggaatcc gttctcttcc ggcagatata catattttat
     3541 atagctgcct gctgcttccg ccacatactc gtatccgtaa aaggatggca cgggaatcac
     3601 aatattccgg ggcttaagtg catgcacaac cgccagaaaa agttctgatg ccccgttgcc
     3661 aaacagcaga tactcctttg ggatttctct gccacattct ccggataaca tccggctcac
     3721 ggcttccgtc agggccgccg cagtaatatc cggatattct cccacatgct gcactgcttc
     3781 atgaatcgcc tgcaccgccg catccggcat ccctaaagga ttgccgttta ccgaaaaatc
     3841 cagccgtatc tgtctgctgt aaatatcacc gccatgtatg ttctgctgca ttttccttcc
     3901 tttccggttc ctgttaccat aggacttttc atgtcctcta tcgtctgcaa atcatcaaaa
     3961 gctttcacgc ccggccaaac tctgcttttc acactgccac cgcaatcagg atccccagac
     4021 acagaatctc acagatccac gccgtccggt tcatcaatcg gttcgttctg cggatatcct
     4081 ccggttccac tgcccttgtt ggatccccga tatagggctt tttcacgacc ttgccaaaat
     4141 aactggcatc tcccgccagc tggattccca gcgctcctgc gcacacggat tccgtctggg
     4201 cggaattggg gctggcatgc tttctgttgt ctctgcgcca gatacgccat gcctgctttc
     4261 cgttatagct tttcccgctg ataaaagccg tcccgaccat caacagcgcg ctgatcctcg
     4321 ccgggagaaa attcatcaca tcatccagct tcgccgcggc tctgccgaag tgaagatact
     4381 tctcattctt atatcctacc atagaatcca tggtattgac tgccttatat aaaaatccca
     4441 acaccggccc tcccagtgca gtatacagca tgggggcaat cacaccgtcg gacgtatttt
     4501 ccgccacggt ctcgatggcc gcctttgcca cgccttcctc tgtcagatgt tccgtgtccc
     4561 tgcctacgat catggatacc gcttttctgg cttcctccag gctcccggca ttcaattgtt
     4621 tccacacctt gctgctctcc acctgaagac acctcgccgc cagaatctgg taagtcatga
     4681 ccgcttctat gaccactccc agtcccagat gcatcgcata agcaccgaat aaaatcagtg
     4741 ccgacagcat tcccgtaaac agtaaaacga ccaccaccag aagttttccc tgtctgcgct
     4801 ccgccccctc tctctcggtc gaattgccct gtggattgcc tgctcgcagt gtcttttctg
     4861 tcttcgctat cagccagccg attccccgga tgggatgggg cagaaaatag ggatctccaa
     4921 agtgctgatc cagtaaaaat cacgcgccga atgccagata atgagatttc atcaatatgt
     4981 t
//
LOCUS       DAWDHD010000187         4939 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_989_length_4939_cov_12.128378, whole genome shotgun sequence.
ACCESSION   DAWDHD010000187 DAWDHD010000000
VERSION     DAWDHD010000187.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4939)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4939)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4939
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_989_length_4939_cov_12.128378"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1567 a          943 c         1268 g         1161 t
ORIGIN      
        1 tcatatcaat tccggatagc agcaaagaag aaatgtatga actgacaaaa aaagaatttc
       61 tgcgtgaaaa tggtgtatgc aatggtgata caacaaaacg aacagatgtg tataacaatt
      121 tgtatcgcaa aatgtccaaa aaggacaggc tggctgccgg gtacaccctt gaaaaatatg
      181 agagaattta cagacaggct ttttatgatg ccgcaaaaaa ggctgatcct aactgggaaa
      241 ttggtaagcc aatcaaagct ggagcacttg atgatgttac aagagagact gcagagacag
      301 gaaaatcccc tgcacaggca acacttgata ctaaaatcta aagcgtataa actattcttt
      361 ttgagaaaca aaggtaatca gctatattga attaacagat ggtataaaag taatggaagg
      421 ctctcttatg aaaatagaag caatttcata aaatcttact tacacacttg acaatatccc
      481 tctgaggaaa cagatgaaaa agatattaca gagaatcgga ctgctggcag cggcggtgct
      541 gctgctgtgg ggttggcagg ggatgaatgc taaggcggac gattatacgg gaaactggac
      601 agttgataga accggattaa aaaactgtca tataatgcta agtactatga tagatagtaa
      661 aatgtatgat tgtttttcta tatacccggg agatgacagt tataatggaa aagaatattg
      721 gtttttgaca gcaccgaaag ttgtatattc tacggatgat gttaatgaaa tagaagcaag
      781 tgcaacctat gacagcgttc aacaatgtta ttattatcgg cttccaaatc atggaacagg
      841 taaaatagtt atgacaggtg aagcagtcaa tctggaattt cattctagct tgtcggaacc
      901 ggcacagttg gtttcaaatg atgagaaatc ttattttttg tatgatttaa aaataaattc
      961 atattatgat ttttctaaag taaatgtttc atttaatttc tatgatggag cgggttaccg
     1021 tgcagatgac atttttgatg caaaaatcat aaattatagt gaagataaca gaactgcaac
     1081 agtgaagatt agctataaaa cagaaaactg taccaaagtg gcgggatata aggtgtatgc
     1141 aaaagcagta tatgatgatc cacaagggga tgaacatagg aaaacagata taatgtcgtt
     1201 taatgttggc acagttgatc ggacaatctc cagtctggac atggtagtca ccccaccggt
     1261 agccggacag cccttaccta aaaaatggca gattaatacg gaaggaatta ctgatgttac
     1321 gaagaacagt attttgtggt tgaaagaggg aaatgagaat gaggcggcaa tcggcaatgc
     1381 tgaaccgaac acaacgtatt atgcaattct gaccttggtt gcagaacgtg gctataaatt
     1441 caatcatgac agcatctgga ttgtcacaaa tccacaagtg gagtatacgt caattgactt
     1501 agctgaggga cggtatctga cattgaaata tacctttaca acgggagaga ttacaaatac
     1561 tacagataat acagatacat cagcttctga cagtggtacc gatgacagcc aggaagatcc
     1621 ctataagatc atcgaaggca ccaagggtac ctggaacggc agcaccacgg aaggcctgac
     1681 catccgcggc gatggtgatt ttgctaaatt cgccggtgtc cgtgtagacg gcaactggat
     1741 ccccagtgcc cactacgaag caaaatcagg ttccactatc gtgaccctga agccttccta
     1801 cctggcatcc ctgtcagagg gtgaacatac tgtggacacc atgtggatcg atgattccgc
     1861 cagcaccaca tttacagtag ctgcaaacac cgcagctgca cagccggagc tggacagcgt
     1921 gcccaagacc ggagaaggtg tgatgggatg gatgcccgag gtactgctgt ttgcagctgc
     1981 aggtctggtg acattcggcg gacttctctg cagacgtagt aaaattcaac tttaaaggga
     2041 aaaaccacat aaagattgtg taaaaagtag atatattttt aaattgacga gcacatggct
     2101 gttggatata ctataactgt atcgcgtttt aaacgacgtg atacggaaag caggatccga
     2161 cagccatgag cagtttacaa aagaaagaaa cattattaat cgttgatgat tcaaaatttc
     2221 aaagagtagt gatcagacag tctctcgggg agcattttaa cttcgcagag gctgtttccg
     2281 gtgaagaatg tcttacgatc atggagaagg aaagcgatgc cattgatctg gtgctgctgg
     2341 atctggtcat gccggggatc gacggctttg aagtactgcg ccgcagacag aatatggagg
     2401 gcttcaagga cacgccggtt atcgttctga cgaatacgga atcggtgtct tcccagtcgg
     2461 aagcatttgc actgggggcg gatgaattca ttatcaaacc ggtagatacc cggatagcgc
     2521 ttacccgcat caacaataca ctgggggtaa aacgcaggtt acagaatagc agagatgagc
     2581 agaaggcctg gaagacaaag tcgcagatcg acgagatgac cagtctgttc aacaagatga
     2641 cagtagagaa gctgatcacg aagactctga cagagcatga gaatggcctg catgctctta
     2701 tggtgataga tatcgataat ttcaaatccg taaatgatgt atacggtcat actatgggag
     2761 accatgtgat cagcgtcatt gcaggagtca tttcttcaca gttccgcagc acagattatg
     2821 tgggacgtat gggtggagat gagttcgcag tattgatggg agacattccg tccaaagaga
     2881 tcgcactgat caaggcagag aatctggtca acctcgtaaa atataaagag aacttatcca
     2941 taccggaaaa tatatccatc agtgtgggaa tagcattttc cgatccagag gatcattgct
     3001 actatgatct ttcggcaaaa gcagatcagg cattgtatgt atccaagaag tcgggaaaag
     3061 gacgctacag tgtatatggc gaagaaaacc atgagcagag cagaaaacag ctggctatag
     3121 tgtggtccgg gtcccgaaat gtcaccagta tgatagaatt tgcgttgccg gattccgtac
     3181 agctgaaaca ggtggattcc gtggaaatga tcagagagtg tatggaggaa gcggcggaag
     3241 agggaattct ggctcttgtg gtggatgtat cggaagaaga ggatcaggga caggcacgct
     3301 ggcaggaact tcggaaagta cagacggaac agacatttcc gaccatcgca atctgtaggg
     3361 aaggcaatct gaaacagatg aaaaatgccc tggagaccga agtaatccag gatcttttgc
     3421 tggctccgct ggaagcaaat gcattgaagc ggcgtgtgaa gatctggatg caaaattgca
     3481 agctgcgata aactttgtta taattgactg gagcatatct tagaatgtgc ttcagtcttt
     3541 tggtatcttt gcaatagaaa cagaggatgt aatattatgg atttgtttga atacatgaga
     3601 agcaccaata tggaaaagga agctccactg gcggccagaa tgcgtcccag aaccttggat
     3661 gaggtcgtgg ggcaggagca tattatcggc aaggacaagc ttctgtatcg tgccatcaag
     3721 gcggacaaga tcagctccat cattttctat ggaccgcccg gtaccggcaa aacgacgctg
     3781 gcgaaagtca ttgccaatac taccagtgct gaatttcaac agattaatgc cacagtggcg
     3841 ggcaaaaagg atatggaaga agtggtggca aaggcgaagg aactgcaggg aatgtacagg
     3901 aagcgtacaa ttctgttcat tgacgagatc caccgtttta ataaaggaca acaggattat
     3961 ctgctcccgt atgtggagga cggtacgatc attctcatag gagctactac agagaatccc
     4021 tattttgaag tcaacggcgc tctgatctcc cgctcggtga tctttgaatt gaagccgatt
     4081 ccgagggaag ccatcaaaga gcttttgaaa aaagcagtct acgatacaga ccggggaatg
     4141 ggagcctaca acggcaccat agacgaggat gcactggatt ttctggcaga tatctccggc
     4201 ggcgatgcca gacatgcact gaatgctatt gaactgggca tcatgaccac agaaccggga
     4261 ccggatggca agatccatat tacgctcgac gtggcacagc agtgtatcca gaagagagcg
     4321 gcgagatacg ataaaaacgg tgacaaccac tacgatacga tttctgcatt tatcaagagt
     4381 atgcgggggt cagatccgga tgcggcagta tattatctgg caaggatgtt aaatgctggc
     4441 gaaagcgtta cttttattgc aagacgtatt atgatctgtg catcggagga tgtgggcaat
     4501 gcggatcccc aggcactggt gatggcagtg aatgcttctc tggcggtgga gcgggtaggc
     4561 atgcctgagg cgcagatcat tctggcgcag gcagtgactt atattgctac agcacccaag
     4621 agcaatcgta gctgcgaagc ggtatttcag gcgatgcagg aagtacaggc cacaggagat
     4681 ctgccgatcc ccgcacactt gcaggatgca cattataaag gagcagcaaa attgggacac
     4741 ggaacaggat ataaatatgc acatgattac ccgaatgact atgtggaaca gcagtatctg
     4801 ccctatgaat taagtggcag ggagttctat cagccctcgg gtaacggtta tgaggtgaag
     4861 atcagagagc atatggcgag aatcaaaaaa gaagccgcag aggcttcttc taaaaacaaa
     4921 gaatcataaa atcaggtac
//
LOCUS       DAWDHD010000188         4930 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_991_length_4930_cov_11.834667, whole genome shotgun sequence.
ACCESSION   DAWDHD010000188 DAWDHD010000000
VERSION     DAWDHD010000188.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4930)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4930)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4930
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_991_length_4930_cov_11.834667"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1357 a         1189 c         1331 g         1053 t
ORIGIN      
        1 gaaaaatcta tatttacaaa tgagaaaaat aggtgtatag tacagaaaag gtcgcaatga
       61 ggcgctcgaa agagtctcat tgcgaccttt tttgttacta ttacggtctt gcgaatgagg
      121 tcctgaatag taacagacag attaagaagc gagaaataaa aaacccggaa aggagaacta
      181 tatgtttgat gtaaaacagg agctgcccaa ggcaccgtta tatcgggaag aatttgagca
      241 tgataactgc ggtatcggag cctgtgtcaa tattcacgga gagaagagcc gcgcgaccgt
      301 agagaatgcg ctgaagatcg tagagaatct ggagcacaga gccggtaagg atgcggaagg
      361 caagaccggt gacggtgtcg gtattctgac ccagattccc cacaaatttt ttgcaagagt
      421 cacaaaggct ctgggcatgg acatcggcgg agagagagaa tacggtgtcg gcatgttctt
      481 cttcccgcag gaagagctgc gccgcaacca ggccaagaag atgttcgaga tcattgtgga
      541 aaaagaagga atggaattcc tcggctggcg tgaagtgcct accttcccca atgtgctggg
      601 acataaggcc gtagagtgca tgccctatat catgcaggga ttcgtgaaaa agcctgccga
      661 tgtggaaaaa ggactgccct ttgaccgcag actttatatt gcaagacgtg tcttcgagca
      721 gtcttccgat gatacctatg tagtttctct ttccagcaga acgatcgtat ataaaggaat
      781 gttcctggta ggccagctgc gtaccttctt cgcagatttg cagagtgaag attacgaatc
      841 cgccattgcc atggtacact cccgtttctc caccaatacc aatcccagct gggaacgtgc
      901 ccatccgaac cgtctgatgg tacacaacgg tgagattaac actatccgcg gcaatgccga
      961 caagatgctg gccagggagg agaacatgga atccgagcat ctgaaaggcc agctgcacaa
     1021 agtactgcct gccatcagta agaccggttc cgactccgct atgctggaca acacgctgga
     1081 attcctggta atgagcggca tggaactgcc tctggcggtc atgatcgcta tccccgagcc
     1141 ctgggcgaac aacaaggcca tgtcccagac caagagagat ttctaccagt attacgctac
     1201 catgatggag ccctgggacg gccccgcatc catcctgttc tccgacggag atatcatggg
     1261 tgcggtactg gacagaaacg gcctgcgtcc ttcccgttat ctgatcactg acgatgatac
     1321 actgatcctg tcttccgagg taggtgtcct tcctgttgat cccacgaaga tcaagttaaa
     1381 ggaaagactt cgtcccggca agatgctcct ggtcgatacc gtacagggca gagtcattga
     1441 cgatgaggag atcaaggaaa aatatgcttc cagacagccc tatggtgaat ggctggacaa
     1501 caacctgaca gaattgaaga gtattcacat cccgaaccag cgtgtccctg agtacaccta
     1561 cgaagagcgt cagcggatgc agaaagcttt cggttatacc tacgatgaga tgaaacagag
     1621 cattctgcct atggcaaaga acggcagtga agcgattgcc gccatgggtg tggatacgcc
     1681 gcttcccgtc ttaagcaaga cgaaccaccc gctgtttcat tatttcaagc agctgtttgc
     1741 acaggtaacc aacccgccca tcgatgcgat ccgtgaggag atcgtgactt ccaccaccgt
     1801 atacatcggt accgagggca acattctgga ggagaccccg aagaactgta atgttctgaa
     1861 ggtcaacaac ccgatcctga ccaacacgga tctgttgaag atcaagaaca tgaaggtaga
     1921 cggattcaaa gtagaagtcg tacctatcgt atattacaag aacaccagct tagagcgtgc
     1981 cattgaccga ctgttcgtgg aagtggacag agcttacaga gacggcgtga acatcctgat
     2041 cctgtccgac agaggcgtgg atgagaacca tgtgcccatt ccttccctgc tggccgtatc
     2101 cgccctgaac cagtatctgg tacgcaccaa gaagagaacc agagtcgcac tgatcctgga
     2161 atctggcgaa cccagagagg tacaccattt tgctacgctg ctaggttacg gtgcctgcgc
     2221 catcaaccct tatctggcac aggataccat caaggaactg atcgagagca atatgctgga
     2281 taaggattac tatgcagcag tggatgatta caacaatgct gtcctccacg gcatcgtcaa
     2341 gatcgcatcc aagatgggta tcagcaccat ccagtcctat cagggttccc agatcttcga
     2401 ggctatcggt attgcaccgg aagtgatcag taagtatttc tccaacaccg tatgtcgtgt
     2461 gggaggtatt accttaaaag atattgagca gcaggtagac gacctgcact ctcaggcatt
     2521 tgatcctctg ggactgaaaa acgatctgac cttaaacagc cccggacatc acaagatgcg
     2581 cagcggcggt gacgagcatc tgtacaatcc cgctaccatc catatgttac aggaatccac
     2641 cagacgcggt gattaccaga tgttcaaaca gtacactgcc atggtaaacg acgaagattc
     2701 catcaagaat ctcagaggac tgatggactt caattatccg aaaaagggtg tgcctattga
     2761 ggaagtagag ccggtagagt ccattgtgac cagattcaag accggtgcca tgtcctacgg
     2821 ttccatttcc aaagaagccc atgagaccat ggccatcgcc atgaaccatc tgcacggcaa
     2881 gagcaattcc ggtgaaggcg gtgaggacct ggatcgtctg accgtcggac cggacggact
     2941 gaaccgttgt tccgcaatca agcaggtagc cagtggacgt tttggtgtta ccagccgtta
     3001 cctggtaagt gctcaggaga tccagatcaa gatggcacag ggagcaaaac ccggtgaggg
     3061 cggacatctg cccggaggaa aggtatatcc ctggatcgcc aagaccagac actccacacc
     3121 cggtgtgtcc ctgatctctc cgccccctca tcacgacatt tattccattg aggatctggc
     3181 gcagctgatc tatgatctga aaaatgccaa cacccaggca agaatctccg taaagctggt
     3241 atccgaagcc ggtgtgggaa cggtcgcagc cggtgtagcc aaggcgggag cccaggtcat
     3301 cctggtatcc ggctatgacg gcggtactgg tgcggcaccg agaaactcca tccacaatgc
     3361 cggacttccc tgggagctgg gactggcaga gacccatcag accctgatca tgaacggtct
     3421 gcgtaataaa gtccgcatcg agaccgacgg taagctgatg agcggcagag acgtagccat
     3481 cgcagccatt ttaggtgccg aggagttcgg ttttgccact gcaccccttg tgaccatggg
     3541 ctgtgtcatg atgagagtat gtaatctgga tacctgcccg gtaggcgtgg ctacccagaa
     3601 cccggagctg cggaaaaact tccgcggtaa gccggaatat gtggaaaact ttatgaaatt
     3661 tatcgcacag gagctgcggg aatacatggc agcactgggc gtgcgttccg tagatgaact
     3721 ggtgggcaga actgatctgc tgaagagaaa agagcatctg accgatcgtc aggaggaagt
     3781 ggatctgacc cagattttaa gcaatcctta cgagggcagc aagcagaagg tgacctttga
     3841 tcccaggcag gtctatgact tccagttaga gaagacactg gatgagaagg tgctgttaaa
     3901 gcagttagga aaagccatcg acgcaggaca gaagcgcagc atcgaagtag acgtcagcaa
     3961 caccgatcgt gccttcggta cgatcctcgg aagtgagatc accagaaaat tgggcgatga
     4021 tgtggaagag gatacctacc atgtactgtg caagggtgcc ggcggacagt ccttcggagc
     4081 ctttatcccc aagggcctga ctctggaact ggtaggcgac agcaacgact acttcggcaa
     4141 gggcttatcc ggtggtaagc tggtggtata tcctcctcag ggcagcaggt tcaaggctga
     4201 ggaaaatatc atcatcggta atgtggcact gtacggtgca acctccggta aagcattcat
     4261 taacggtgtg gcaggcgaac gtttctgcgt acgtaactca ggtgccatcg cagttgtcga
     4321 aggtgtaggt gatcatggtt gtgaatacat gaccggcgga cgtgtggtag tcttaggacc
     4381 caccggtaaa aactttgcag ccggtatgag cggcggtatc gcttatgtac tggatgaagg
     4441 caacgatctg tacaagcgtc tgaataagga aatggtatcc tccagcgaga tcacatccaa
     4501 atatgatgtg ctggagttga aatccatgat ccaggagcat gtggcactga ccaattccgc
     4561 caagggtaag gaagtgctgg acaactttgg agagtatctg cccaagttca agaagatcat
     4621 ccctcatgat tatgagagag tcttaaagac catcgtccag atggaggaaa agggactgag
     4681 cgcagagcag gcacagatcg aagcattcta tgccaacacc agaaagtagg aggccagtgt
     4741 gaaaagtaat cttgatagat tgcttttcac acgactaatt tgtgccgcgg gcgccacaaa
     4801 ttagtttatc gcagaactaa atatgaaatt tagttcgcga ttcttccgac gccgaagcgt
     4861 ctgcagaatg gagagagtca tgggaaaacc aacgggattt atggaatata aaagggaagt
     4921 cagcgtggct
//
LOCUS       DAWDHD010000189         4924 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_994_length_4924_cov_11.848018, whole genome shotgun sequence.
ACCESSION   DAWDHD010000189 DAWDHD010000000
VERSION     DAWDHD010000189.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4924)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4924)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4924
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_994_length_4924_cov_11.848018"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1325 a         1140 c         1277 g         1182 t
ORIGIN      
        1 tcgctgagtt cggcggtaag gcggagatcc gtgataccaa gaagaagcgt gaagtggtaa
       61 ttaccaacga agagaccggt gaatccaagg catatctgat tccttacggt tcccgtatca
      121 aggttatcga cggccaggta ctggaggccg gtgatgaact gaccgaaggt agtgtaaacc
      181 ctcacgatct gttgaagatc aagggtattc gtgccgtaca ggattacatg cttcgtgaag
      241 tacagcgtgt atatcgtctg cagggtgtag atatcgcaga taagcatatc gaagtcctgg
      301 tacgtcagat gctgaagaag gttcgtatcg agaataacgg cgataccgag ttcctgcccg
      361 gaactctggt agatgttctg gatttcgaag aaatgaacga gaagctgacc gctgaaggca
      421 aggagcctgc agaaggtaag cgtattatcc tgggtattac caaggcagca ctggcaaccg
      481 agtccttcct gtctgcagca tccttccagg agaccaccaa ggtcctgaca gaagctgcca
      541 tcaagggtaa ggtagataat ctgatcggtc tgaaggagaa cgttatcatc ggtaagctga
      601 ttcctgtagg aaccggtatg aagagatatc gcaataccag actgagcacc gatgccgtag
      661 agaccattga cttcggtggt gatatgtacg gtgacgatgc agaactgaac ctgagcgacg
      721 gaagcgaact ggatgcagaa ggtgcagaac tgaatgaaga tgtgaacaca gaagcagaaa
      781 ctgtggaagt gggaacagac gctgataatc agtaaaataa aaagacttta ttaagaaagg
      841 ctctccggga ttttcccggg gagcctttta gaatcggaac aattattgca attcttctgt
      901 attcagaacc tgccaggcag tgatccggta aaaggtatct tcatcagatt ccggatagag
      961 gatctcaatg gtcacctgca gggtctgtaa gtcggagatt ggaatcacgt aagattttcc
     1021 gtgacctacc gccacaaaat aggcttcttc gctgtcactg gatgcataca tgcgttgcaa
     1081 cgtcttatcc atacctgcca gggattgttc tgcctgattg caggcggcat aataagcggt
     1141 ggtacgttcc agcacacggg cgctgagttt gctatcagcg ttggcactga cgatggacag
     1201 tacggcaaaa gacaccaggc agaggatcac gaatacaaga aggatcgagg cggatccgat
     1261 attcatgccg aattgttttt ttctggagga attattcatg ggatcccctc ctttctgcgg
     1321 tgtgatgcat gacagagagt gtgtaaatgg gagattccgg tgtagataca tcatagacgg
     1381 tgatctcgcc tttcatgatt ccggtctctt ctgccggagt gctttttagt tctgcataaa
     1441 agccggcgtt atcttcggca gtgcacagag tccagctgct gtcaaaggca aggcggagag
     1501 tgttttcatc ggtcagctcc gatggggaga acaaatcctg catggcagcc acattgccct
     1561 ccagagagag ataggactct gccagattct gtgcctgaat gacagcatga ttctggttta
     1621 ccgtctgtgt gctcaaaaga tgtgagcggg caaacaactg gatacagacg gcactggcca
     1681 gtgaaaaaaa caggatcgct atgatcattt ccattaaaaa taaactggat ctggaatgtt
     1741 tcataataag ttcggaatcc tttctgttcg gtttctattc tgtggtagta ttacagtgca
     1801 cgtggatata aaaagtatgg tcctctccgg aggcggttct cagggatatc cggatcagat
     1861 cgtccgacgc ataggataag gagaactcct gcaagtccat aatatccgta ccggcaggca
     1921 atacctgtcc gccgagagaa gctccttccc gcataaaaag ttcttttaaa tgtccgtcat
     1981 acagatagag gtaagtacag taggtttctt cgtcaatttc ggaaagcatg cagagcgccg
     2041 gaacattttc cagatcggtc aggaaaatac tgtcagcggt atcgttctgc cgcacctttt
     2101 ccatgatata agacaccgcc gtccggcttt cgtaattgac gcccatatct tctacagtgt
     2161 gctggtatac atctgctccg atggtaacaa gggcaagtgc ggagacagcg aatacaccga
     2221 acaacgccag aacgaacaag atatccacaa taaaatgttt ttcctgcttc tgatgatttc
     2281 tattgttcat atgtcagaaa ctcctttagt gagcaccggt ccggcgaagt acagtgactt
     2341 ccggaagaag gttgctgcca taatattcat agtcaatgaa gaagctgtcc tcgtcataca
     2401 acagtccgta atgctgctcc agatattcca gagaaggagg ataacagcct tctacggcat
     2461 agcactggga aatgctgcgt tccaatgcgg tttccagact ttcctgctgc ttgctcagag
     2521 tagattcacc gacggagccg atgccccgca gaaaaagaac aaacaatatc acaaaggcca
     2581 gaatgggaag taaatagatc agcttacgcc cgagggacat acttttttgc tcaaaacggt
     2641 tcatagtatc gtactcctat ccgatgctgg tcataatacc cataagaggc aggatgaccg
     2701 ataacaggat caagcccacg atcaccgaca gaataatgac aagggtcggc tccagaatgg
     2761 caatgatgga ttgtagtctg cggttggtat tttcctcata acgatccgct atttttttga
     2821 gcaccacatc tacgttaccg ctgcggaaac cgacagatac catctgggaa tacaggtggt
     2881 tgaaaatgcc ggcaccggtc agagcttcgg caaaattggc accggcatta atggcttcct
     2941 tacagctcag aatcttctgt ttcattttct cgttgcctac cagagcggcc accatatcca
     3001 ggctggaata ggtatccata ccgctgctta aggtcagtgc cataccgctg gcaaaacgtt
     3061 cgcaggccac actttcataa aaacgtctgg tgagggggaa ccagttcagg aaccgggcgg
     3121 tgacacgttt gccggtctgg gttctggtag caaaaaggta cagtaacaac aatatgcaca
     3181 gaatacttac gaaaataaag gaataccggt tcagatggtt gccgagacgc aaaagtgatg
     3241 cagcaaaccc agtcatctca ctgcccagct ctatatagac ctgctcgaag atcggcatga
     3301 cgcggctgac cagtaccacg atgacagcgg ccatcatggc gatcatgata aagggatatg
     3361 ttacagcatc ccggatactg cctgcgatgg catcctcttt ttcataataa tccgccagag
     3421 agctcataca cacatcgaga ttaccggatt cttcacccag agcgatcata tgcaggcagt
     3481 aatcggggaa gaccttggtg gcttccagtg ctttgtgaaa gctttctccc tgtcggcata
     3541 cggtaaggat ctcctcgaag atcgccttgc cttcgggaga ctgggcatca ctttgcagga
     3601 tggccatgcc ctccacgggc ggaatgcctg cctgaaacag catggcagcc tgactgcaaa
     3661 aagaagcgat ctcttcatta gaaagtagtt ttttctctgt ggtcttcatg gcgttttgtt
     3721 ccctttcact aatacatttt ctgcaggata taattactgc cgtcaccgat gaactttttc
     3781 agagtcttgt catccgtgtt ggcaccgaac gtaggatcca ccagggtcca gacattgccg
     3841 tcaaattcta tgatgccgtt cagccatccg gtatcagcgg tgtagacact gatccaggca
     3901 tggtaggcat cctgggcata gccgacctcc agccgggtcg ggatccgctg gcttcgcagc
     3961 atgcttgcca ttaccgcggc ataatccaga cagataccgg tgccggaagc aagaatggca
     4021 tccacatcgg aggtatagcc cgtaggagga tcggaggctt tgtcgtagtc gtaagtaata
     4081 ttctgggtaa tataatcata gatccgggtg atcacttcca gatccgagga agcaccctcg
     4141 gcaagttcct gtcccttggc taccactttg ctgtccgcag taaaattcac gtactggttg
     4201 gggtagagaa aagggctgaa tgcatccgta atctgtacat cgaggtctgc atacaggcag
     4261 gtggcataat tggtaccgga gatattttca tagatcgtca cgtcataata tccgtctccg
     4321 gaagacaagg ggaaagtctc atagccgcca ccgtgcagat cgtaagtata gacaacggta
     4381 tccggcccgg taatgcgcag tttgaccttg ggacaggtgc cggtgtaatt ggcacagaca
     4441 tagccgtcag acatatgaga atagtcaatg acagccacat cattggaagc tgttgctgtt
     4501 ccgtcggccc ggggcacaag acagacaggc gtggcatccc ggagaggctc tgcgtcagtt
     4561 acggcttccg tttcaggaga agaggcatcc gcagagctga tattcgtatc cgcagaagaa
     4621 ggaactgcgg aggattgtga tggttcgccg taagtgtctg cgacgggggc atccacaggc
     4681 agattgtcag agccggatcc gcaggcacat aagccgattg tcatggaata tgtaattagc
     4741 aaaatgcgca gtcgcgcaga taattttggc atccgcataa aaatcgttac atcctgttgt
     4801 tattgttcca ggcccgttac attatcggga gtaaaaagca ggtggagagt ctcaccgttt
     4861 ttgacgggga cataaatatt ggcttcctca gaaagaaaag ggaaattgat cacacccttg
     4921 gagg
//
LOCUS       DAWDHD010000190         4846 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_1013_length_4846_cov_12.065122, whole genome shotgun sequence.
ACCESSION   DAWDHD010000190 DAWDHD010000000
VERSION     DAWDHD010000190.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4846)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4846)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4846
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1013_length_4846_cov_12.065122"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1481 a          936 c         1266 g         1163 t
ORIGIN      
        1 ggttacagaa ccggaagtgg gaaatggcaa tgttgtcctg gctctccccg caggctatga
       61 cagaagtgca aggctgtata ttgacggtgt agagttttcc ggcacagcat ggcagaatga
      121 cgagtcgaaa cggctgataa gcatcggaag cacaggatcg ttggggacta cggcacagac
      181 agctatggca gttcaataca atgcatcagg cattcccacc ggcatgtatg tatggagcct
      241 ctcttataat ggaagctatt atacggctac ggcgatccct gagtttgaga atcttttttc
      301 ctaccacgga ttttctgtgc ggtataccgg caacaccgga ctgagatgta cctttggtat
      361 agattctgca aaaaaacagc agctgaccgg tgcttccgga ctgcagggat accggatcaa
      421 ggaaatcggt acgctgatta tgcgcccgga tctctataca caatatccca tggtctatgg
      481 aagcaataaa ttaggtggag gaaaaacata tggtgtcatc aacggcagat tcagcgataa
      541 ggtcatcaga agagtgaatg gcagagatca gtttgcaaat gtccttacga atcttccacc
      601 ggctcgttat aacacagcgt atatcttccg cccctataca gttatggaaa aggacggtag
      661 caatgtggta atctatgggc cggaaatgtc ccgtagtatg tacactgtct gcaaacagat
      721 tttgaaccgt ggagacttcc gaccgggaac gtctggttat aatttcctga aaaatattgt
      781 ggacagcgta gaaaagtaga gataggagca attctgaatg gaaagtaata ataaaaataa
      841 gaaaaataca gtgtggatca tactgacctg tgtatttgca ctgacgacat tagtgttggg
      901 aatggcatta tatcgttctc agagtgacag gaaactcctg gagagtcagc tggcgggagc
      961 acaggatgtg gtggcagtga caacggctca gcaacaacag ctggcaggag cagataattt
     1021 acaggaaatc cgcattatga acggtatatt acagcaacag gataagcatc aacaatgggt
     1081 agatgtggca cctgtagagg atctgctgca ggcggatccg gtactggcag ggagaaataa
     1141 gatgcaggaa ctgatcacac agaaccggga agcggtgcaa aatggaaccg tcagtgcaga
     1201 acagctttcg ccgctgatgg ctaaagcagg taccctgaca ggcgcagcac tggcatccgc
     1261 atcccgggtg gagagcagca ataacaaaac aactgcacag ccaccgaaag cacagacagg
     1321 cagtaatagt gcgacaacgg taacagcacc ggcaacaact ccggcggcaa cacctgctcc
     1381 ggtacagcag ccggataaca ataataactc tgataataat cattccgaca atgactcttc
     1441 agataatgga agctccgata atggcagttc agacagtaat tccggtggag attcctctga
     1501 tagtggagat ggcgaagata tcggatggac ggatgagatt ctgtaaaaat gaaaattccc
     1561 aaaccatagt ttaaagaact gatggtttgg gaatttttgt gaagatgtta aacagtaatg
     1621 ctttctataa taattatttc tccaaatcaa ttgtcttcag taattcccga atctgttcca
     1681 cgctcagcca ctcagtatta ttgccagagc tgtaggagaa gccttccggt actctcttgc
     1741 cggtaggaac ggcacgtctg gattcgctcc ataccatttg gggatatacg atgaagtgct
     1801 tatcgtattc ataggtgttc agggaatctt ccacggtaac catgatctca tgaagctttt
     1861 cgccctcacg gattccgact tcaggcatct cacaaccggg cagcattgcc tgtgccagat
     1921 cagtgatctt aaaggaagga atcttggaga tgaaggtctc accgcctttg gattcttcca
     1981 aagctttaat taccagttcc acaccctgct gcaggctgat ccagaaacgg gtcatgcggt
     2041 agtcggtgat gggaagttcg gtaccgccgt ttttaataat gttatggaag aacgggatca
     2101 cggatccgcg gcttcctgct acgttgccgt aacgaacgat ggagaagcag atgtccttgc
     2161 ttccggcata agcgttggca gcgataaaca gcttatcgga taccaacttg gtgccgccat
     2221 acaggttaac ggggttaacg gccttatcgg tagataaagc aacgactttc ttgacaccgg
     2281 tatccagtgc ggcatcaatg acattctgcg caccgtggat gttggtcttg atagcttctg
     2341 cgggattgta ctcacaggcg ggaacctgct tcagagctgc ggcgtggatg acatagtcga
     2401 caccctcaaa tgctctggtc agacgctctt tgtctcttac atcgccgatg aagaaacgaa
     2461 gcttgtccgc atactgctta aattcgttct gcataatgaa ctgtttgaat tcatcgcggg
     2521 aataaatgat gatctttttg ggattgtaat tatccaatac atacttggta aaagctttac
     2581 cgaagctgcc ggtaccgccg gtgatcaaaa tggatttatt atctaataac ataatgtcct
     2641 cactttcaaa actcaaattc tttatcataa taacatattg tatggaaaaa cgcaattatt
     2701 acgcagttta tattatccta atgatagtta acaaataaat aaaagcaaaa gggatgcagt
     2761 atgaagtata ataggaataa aagaatgcct tacggcagta atttaatagc gatctgcgga
     2821 ggtgagaata tacttgaata tgtcaaaacc aaataattta gccgttttat atcctggcct
     2881 catcaggcag tgggattatg agaaaaacaa tggacttact ccgcaggatg ttactgccgg
     2941 ttctcagaag aaagtatatt ggatctgcga gaaggggcat tctttcgaca tggatatccg
     3001 aagcagaacg aaagggcagg ggtgtcctta ttgtaataac agaagaattt tgccgggata
     3061 caacgatctg gccaccttga acaaagaact ggcagcggag tggaattggg agaaaaacgg
     3121 caatctgaag cctacagagg tagggacagg ctctaaaaac agagtttggt ggaaatgtga
     3181 taaaggtcat gaatggcagg cacgtgtgat tcagagaaat ggtggcacgg gatgcccggt
     3241 gtgtgcgaat agaatcgttt taaaatccta taatgatttt gcatcacagc acccggagct
     3301 gctgtcggaa tgggactacg acagaaatga gattgacccg tcagaatgtg catgcttttg
     3361 cagaaaaagg gtatggtgga attgcagaag agggcatcat tggaaggcga ctgtcagcga
     3421 ccggagcaag ggaacgggat gccctaaatg ttcagaggaa cgcagggtat cttttccgga
     3481 aaaggcaatc ctctattatt tgcgtttaca tatagagaat gtacaggcaa actacaggga
     3541 cgagagcata gcgccgtatg aactggatat ttatttgccg gaatatcgtc tggcaattga
     3601 atatgacgga acttacggac attctaaggg aaacggaata gcccgagatc ttcggaaaaa
     3661 ccggaaatgt gaggaaaatc atattgcact ggtccggata cgggagaagg actgtccgga
     3721 actgaatgat tcttctttcg attacaaact ggaagacaga aatggattaa aggatgcaat
     3781 ttattttttg tatcagtatt tattagataa atacgcagtt gagatacaca tgaaagagga
     3841 tgaggtggat ccggaacggg atgcggcaga gatttattca ttgattgcat tcagcgagaa
     3901 agaaaattca attttgaaaa aggcacctca gattgcgaaa atgtggcatc ccacaaagaa
     3961 tggccgggta acgccggaag atatttctgt cgggtccagt aaaaaattct ggtggcaggg
     4021 agattgcggt cacgaatgga tgagtacagt ttcttatgaa atcagcagtg gaaagtgtcc
     4081 ttattgttcc ggaatgcggg ttttacaagg gtttaatgat ctggcaacgg tgaatccggc
     4141 aattgcaaaa gaatgggatt atgaaaagaa tgatgaatta tccccggaga aaattactgc
     4201 cggaagcggt aaaaaagtat ggtggagatg tgaaaaaggt cattcctggt atgcttccat
     4261 tgtttcaaga aatcgaggaa atggttgccc tatctgtgca aatcgtattg ctttaaaagg
     4321 atacaacgac attacttcaa atgaaagact gctgaaatca tgggattttg aaaaaaataa
     4381 tgggctggat ccggcaaaac ttagcattgg atcagagaaa acagtatggt ggctttgccc
     4441 tgtgtgtgga tgcgaatgga aggcaatgat ccgccgacgg gcggagggaa atggctgccc
     4501 ggaatgtggg aagaggatcc gatatgcaaa ttccagaagg aagatggtaa aagacagagg
     4561 gagccttgcg gagaaaaatc ccgctctgct ggaagcgtgg gattgggaga agaatacagt
     4621 cagtccctat gaaatcctgg caggatatac gaaaaaagtt tggtggaaat gcaaacaatg
     4681 cgaaaatgaa tgggaagcca cggtgatctc cagaaatgat ggacggggct gtcctgcttg
     4741 tgccgaaaag agcagagccg cagcgagaca aaggaagctt ttgagtaaaa agcagccgat
     4801 taccatgacg catccggaac tgatgcagga ttgggactat gaggaa
//
LOCUS       DAWDHD010000191         4844 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_1014_length_4844_cov_11.589476, whole genome shotgun sequence.
ACCESSION   DAWDHD010000191 DAWDHD010000000
VERSION     DAWDHD010000191.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4844)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4844)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4844
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1014_length_4844_cov_11.589476"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1204 a         1427 c          966 g         1247 t
ORIGIN      
        1 ctcccaaaaa aactactccc aacgctccca gaaagaacca gccttcttca gatttctgcg
       61 tcctcacttt tgttgtcatc ttcgtcatcg ttcctcctac ccgcgtttct gctgtcacaa
      121 ttctattatt tctccgggtt gtcttctgta tctccggcgc ttatggtcct atcggtccag
      181 aggatcattc ccacaagcca cagagaaccc ggcagcaatc cgttcttgta atccaggcat
      241 ccggcatccc gcatggtata ccacaggatc atgcctgccg cagtgatcac acagactcct
      301 gagatcttcc agaacagact tctgtccggt actgtttttt cttttttctg tcggcgcatg
      361 gcaatgatcc cgcatatcat gcagctcacg accacaacag cgattcccga caggaaccac
      421 cagctgctgt attttagcaa aagctctgtc agccttggat ctcccgtcat cataatgtct
      481 acatttctgc cagagaagga gtcatatccc ctgccgtcca acagcagctg caacagtacc
      541 gtaggcatgg catatccgag gcaatcccag gcgatctgat ggatattgtc tgaagtatac
      601 gtcttcagat gatacccggc atactctctg aatatcttct gtgcttccgc cttgccgaaa
      661 cgatcctcca gcgtcttttg tatgccggac tccatggttc ccgcataata cccggcattc
      721 ctcatctctt tccaggtcat ccaggtacgt agctgttccg gccattcatc gtattcttct
      781 tcctctctca tatgcgtcca gctgaaccgg cgcatcatgg cggcgctcac cgtattctcc
      841 gccttcccat agctgcccgg tgtctgccac atgggcacca gcgtgaataa aattcccgcc
      901 attgccagaa ggagcaaaag ttcccggcct actctctttt tctccgtctt tctgctccgc
      961 agctgccaca gatgtatcag ccacagaaca ataagcggta ccagccccag atacagattc
     1021 tccaccacac acagtgtact gagcgcccac cagattccgg cttcgaacag accatgtaac
     1081 ggtctggccg gcgctgtcgt ctcaccatcc tccgccacag catcctgccg cagggtccgg
     1141 atcaccgccg acagttccag cagtaaaagg gaatatcccg gggaattcgg caatacggcc
     1201 agatgacttt gcatggcaca gggaaaggtc agaagcgcca ggcttcccca gcacaaaaat
     1261 atctttttcc ctgtcactcc gcatgcccgt aaaaatacta caccggcata aaatgcaatc
     1321 aggatctgta ccgtatatac aaaaaaagta taagggatag aacttatgga ttctatccct
     1381 tttacaagca tcagaaaaag agggtagccg atccccatgt agtcatcaca cacaagtgtc
     1441 tctgacgctt tcagccatgt gtagctatcg ccgaattccg gaaaaattcc aaaggatctg
     1501 acgccccagc aaattcccag aagaatctgc acggagacac ccaggaacag aaatctgcct
     1561 gcaacctgtg cagacgctct gccaattttt tgcatgttac tgctcctcta attcaaaaaa
     1621 gtttttcacc agtccacaga tgaaatccac ctgatccagt gtcagcccat agtacatggg
     1681 aagtcttacc aggcgctcgc tctctctggt agtataacgg tcctcgccgt gaaaccggcc
     1741 gaacttctgt cctgcgggag aagtatgcag cgggatataa tggaataccg taagcacacc
     1801 gttctccttc atatagctga taaatcttgt tctctgttcc agatctcttg ccttaatgta
     1861 gaacatatgc gcattatgca cacagccctc cggtacggtg ggcaggtcga tctttcccgc
     1921 tttctttaag ggaagcagct gctcataata gcggttccaa caggcaagtc ttgcctcatt
     1981 gatctcatca gcgatctcca gctgtgcata gagataagcc gcattcatat cactgggaag
     2041 ataggaagat ccgtaattta cccaggtata tttatcgatc tggccccgga agaacttgct
     2101 gcggttggtt cccttctccc tcatgatctc cgcttcttcc acatccttct tatcacggat
     2161 cagcagtgct ccgccctcac ccatgctgta gtttttggtc tcatggaagc tgtaggcacc
     2221 gaagtcaccg atggttccca gtgcttttcc cttgtaagat gccagtacac cctgtgcggc
     2281 atcctctact acctttaagt tatgtctttt tgcgatgtcc ataatggtgt ccatctcgca
     2341 ggccacaccg gcatagtgca ccggaacgat ggctttcgtc ttctccgtga tggcatcctc
     2401 gatcagtgtc tcgtcaatgt tcatggtatc cgggcggata tccacgaaca caggaaccgc
     2461 accgcgcagt acaaaggcat ctgcggtgga tacgaaggtg taagcaggca tgatcacttc
     2521 gtccccgggc cgaatgttac acaaaagtgc tgccagttct gtcgcatggg tgcaggaggt
     2581 cgtcagcaga cattttccgg ctccggtcct ctcctcgatc caggcattac attttttcgt
     2641 atattctcca tctccgcaga tcttctgatt ttttacacat tcctgaatgt aatctgctgc
     2701 tttatccaca taaggcggga cgttaaactt aatatcaatc tctctcataa cacatcctct
     2761 tttctactgt ctgttagctt tctctatgtt cccgcttcca gcttcctacg gctgtcccag
     2821 gcacattttc cgtcacatga ctgataatat atttcggtct tcccttgact tcttcataga
     2881 ttctggcaat ataatggcca atcacaccaa ggcttaacat aataaatccg ccaataatga
     2941 ggatcagcag gatcaccgtg gtaaaacctt ccaccgccgt tcccatcaga tagcgcacca
     3001 gggtctgaat cgccaggatc acactgaaca gaatggatac cccgcccatg atcgtcacca
     3061 gctgcaatgg cagtgtgctg aaggaagtgg cattggtaat ggcatatttc atcagactcc
     3121 agaaggacca cttgctctgt ccgtatttcc gctcctgtac ttcatattcc acagtctccg
     3181 tacggaaccc cgcccagaag gtcaacgccc ggaaaaaggt attgcgttcc ggcagttcca
     3241 acaggacctg tactactttt ctgtccagaa gtttgaaatc ggaagaagca ttcatgtcca
     3301 tcttgatcag tttgctcatg atcttgtaaa aaagtccggc agacaactta tatcccagac
     3361 tctccttacc gcggtcggcc ttgatacctt ccacgatctc ggcaccctgc tgccattttt
     3421 cccacatctg tggaatcacc tcaggcggat gctgcagatc acagtccata acgatcaccg
     3481 catcccccgt agtcagctcc aggcctgcaa aaattcccgc ttcctttccg aaattccggg
     3541 aaaactccgc tccccgtact ctcggattcc tggccgattc ttccttaata cgttcaaagg
     3601 ttccgtcctt cgaaccatca ctgataaata ccagctcata atcgatcccg tgctgttcca
     3661 gcagttcccc cagtactttt gcggtattgg caatattctg ttcttcgtta tatgccggta
     3721 aaacaatcga taataatgac atcctctaat cccttccgca gttctcggtc ttgacataca
     3781 ggattccatc cacaaccctg gtggaattcg ctcccggaaa agtatccatg tcaatgtatt
     3841 cctgcgtcat ataaatctca cccacagtat ccggatccag aaaattcagg gttgcgccca
     3901 gatagttctg cataaatgcc tgataatccg ctcccttata gatcagatag tctccgctga
     3961 gaccgatcat accgtccgtg acttttccgg taatatccgt agtggggaat tcatcatacc
     4021 cgatgacacc caccaatgca atgggaattc cctgatagta cccttccgtc tgctcgatcc
     4081 tgtccagcag acgtacacag tacgcatagg tcttctcgta ttttttctcc agattggaat
     4141 atccgatatt atccgatact ccataattga ataccaggat tccggctgcc agtaatgcca
     4201 cccactggat cccgatatct gtttttccgt cctccgacgc atatctgtcc acaaatgcca
     4261 gcatcaggat cagatataat acccactgat accgcatcag caaatgatag gtcagattcg
     4321 gagaaatcac cagaatcaca ttggtcaaaa gcggaagtcc cgccgccaac agaattatta
     4381 taacgaaaaa tgccggattc ttccaccatt ttcttcgcag catgcttctc accagcagat
     4441 aagccaccag aagcaccagt gccgcacagg ccgtaaaaga gaaaatattg ttcaccagca
     4501 catttccatg caccgtaaat gccagaaaat caacatacat tccccggatc gttgcaaaca
     4561 gccccagacc ggaacctccc tgctccatgg aattgatccc ctgataggta tccagcacct
     4621 ttccctgtaa tttcaacaga atctgcagga ttacgtaata caatgcggct ccggcaactc
     4681 ccatgcccag gtaacgaagt acatagaacg ctttcgtctt ccagcccttc tcttccataa
     4741 aaaagagcag gatcacatat acgcacagca gaatggcaaa gggcagatat gcctgataga
     4801 tcccaatact gaacgcaaga cataccgccc cggccagcca gcct
//
LOCUS       DAWDHD010000192         4774 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_1036_length_4774_cov_11.306845, whole genome shotgun sequence.
ACCESSION   DAWDHD010000192 DAWDHD010000000
VERSION     DAWDHD010000192.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4774)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4774)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4774
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1036_length_4774_cov_11.306845"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1346 a         1054 c         1387 g          987 t
ORIGIN      
        1 cgtatccaat gtgggagatt tgacacactt cagacgtaac aacggccgtg acggactggt
       61 catcggtaac cacgtcttct actaagtggc acacaaaaga acagcgcata cagcagagac
      121 ggtatcccgg aacaggggta ccgtctttta acgtacaggc aggagccgat ccgccggatg
      181 gaatgaggaa tgagaagagc acaatagaat cccgtatcgt gcactaatag cggttgtcac
      241 agaagactat ttgtagtaca ctggatacaa ctacaaaacc aaaggagtga tcacatgagt
      301 gaaatggtaa ccttgtggct tgtggtattg atcgtatcga tcggtgtgga ggtcgccact
      361 ctgggactga cgagcatctg gttcgccgga ggcgccgttg tggcagtgat tgccgccgca
      421 ttgcaggcac cgatctggtt gcagatcctg ttattttttg cagtgtctct gctgctgtta
      481 tttttcacca gacctgtcgc agtcagatat ttcaacaaag accgcgtaag gacgaatgtg
      541 gaaagtatga tcggcagaca ggcaatcgtg accagtgaga tcgacaatct gcagggcatc
      601 ggacaggtaa cggtgggcgg ccaggagtgg agcgccagaa ccgaaaaaga cggattgaat
      661 ctacagcccg gaacggtggt agacatcgtg gcagtcagtg gtgtcaagct gatcgtcaag
      721 gtggatccgc agatgacgaa ggtcaccggg gaagtaccgg agtcacagaa ttaagaatcg
      781 tgtaacacgg atacatgagg gaaaagcgcg tacagaggta cgcagcgtac acgaatgtac
      841 gcagaaggag gaaaaatggt agcattaatc attattgtcg ttttagtatt atggatctgt
      901 gtatcctgta tcaagatcgt gcctcaggca caggcctacg tgatcgaacg tctgggtgct
      961 tatcagtcca cctggaatgt aggattcaat ctgaagatcc cgttcatcga taaggtagcc
     1021 agaaaagtca atttaaaaga gcaggtagcg gatttcccac cccagcccgt aatcaccaag
     1081 gataacgtaa ccatgcggat cgataccgta gtcttttacc agatcacaga tcccaagctg
     1141 ttcacctacg gtgtagagaa tcccatgatg gctattgaga atctgaccgc caccaccctg
     1201 cgtaatatca tcggtgacct ggaactggac cagaccctga cttcccgtga gaccatcaac
     1261 accaagatgc gtgcggcact ggatatcgcc accgatccct ggggtatcaa ggtcaaccgt
     1321 gtggagctga agaacatcat ccctcccgca gagatccaga atgccatgga gaagcagatg
     1381 aaggcagagc gtgagagacg tgaagctgtt acccgtgcag aaggtgagaa gaaggctaag
     1441 atcctggagg cagaaggtgc caaggagtcc gcaatccttc gtgcggaagc tgagaaggag
     1501 aagatgatcc gcgaggcaga aggtgaagcc gaagccatcc tcaaggtaca gcaggcaaat
     1561 gccgacggta tcaagatgct gaaggaagca ggcgcagatg ccgcagtcct gaagttaaag
     1621 agcctggaag cttttgaaaa ggtagctgac ggccaggcca ccaccatcct gctgcccgca
     1681 gagctgcagg gcattgccag cgtggcagag accttcaagg cagccgcagg caaaaaggca
     1741 gaataagtaa actaaaaaac gataaaccaa agccactttc cgggaaatac ggagggtggc
     1801 ttttttatgt cggattcaca aaaaacaatg acgggggata aacgcgaggg gttgaaaaac
     1861 acagaataat gaagtatatt gattacatat agcttgttac aggcaacgtt cgtaacagga
     1921 atatcaatgg acaaaatcag aaaacagagg ctgcgggcgc aaggccggaa agcttcggaa
     1981 aagaggagta ttatggattt aaagggacgc gattttttga aattactgga ctttacaccg
     2041 gaagagatcg aatatctgct ggatctggcg gcagacctga aggataagaa gaaaaaagga
     2101 atccccgtag atactctccg aggcaagaat gtagcgctga tctttgagaa gaccagtacc
     2161 cgtaccagat gtgcttttga agtggcagcc catgatctgg gaatgggaac gacttatctg
     2221 gatcccaccg gatcccagat cggcaaaaag gaaagcattg ccgatactgc cagagtactg
     2281 gcaggcatgt tcgagggtat cgaatatcgt ggcttcggac aggatattgt ggaggaactg
     2341 gcaaaatatt ccaaggtgcc cgtatggaac ggcctgacca acgaattcca tcccacccag
     2401 atgctggcag accttctgac catccgtgaa catttcggac atctgaaggg aatccgcatg
     2461 acctacatgg gagacgcccg ttacaacatg ggtaactctc tgatggtagc ctgcgcgaag
     2521 atgggcatgc actttaccgc atgtaccacc gcaaaatatt tcccggcgaa ggagctggta
     2581 gcggagtgtg aggccattgc ggcacagacc ggcggcagta ttaccctgac cgaggatgtg
     2641 aaggcgggca cgaaggatgt ggacgtgatc tacactgacg tgtgggtatc catgggcgag
     2701 cccgacgaag tatggaccga gcgtattaaa gaactgtctc cttatcaggt gaataaagca
     2761 gtcatggaca atgccggcga gcaggccatt ttcatgcact gtcttcctgc attccatgat
     2821 ctgaagacca agatcggtgc ccagatgggt gagcgtttcg gcatcaccga gatggaagtg
     2881 accgatgaag tatttgaatc cggccagtct ctggtgtttg aagaggctga gaaccgtatg
     2941 catacgatta aggcagtgat ggcggcgact ttatgagtat gaaagcaaag attttagagc
     3001 ttcttcggga aaagaaggat cgggtggcag caggagcacc ggaaagcgag gcctatgtct
     3061 ccgggcagga gctctgcgag cagttcggtg tatcgagaac tgcggtatgg aaagccatcg
     3121 gacaactgaa aaaagaaggc tatgtcattg aagcggtaca gaaccgcggc taccgtctgc
     3181 tggatcagac ggaggaagtc tacaaccagg cggatatcca gagcagatta aagacgaaat
     3241 gggtggcaca tcctttactg ttttttgaca gtatcgattc caccaatatc cgtgccaaac
     3301 tggaagcgga acagggcgcg gagagcggcc tgttggtggt gtcggatatg cagagcgccg
     3361 gacgcggcag aagaggccgg ggctgggaga gccccgcagg gataaatatt tattacaccc
     3421 tgatgttaaa accggatttt tcatcggact gtgcaccgat gctgacgtta gtcatggcac
     3481 tggcggtggc gaagggcatc cggcagacac tgcgccgcga cagcgaagag gcagcagcca
     3541 aggtcggcat caagtggccc aacgatattg tagtggacgg taaaaaggtc tgcggcattc
     3601 tcacggagat gagcatggag caggggtaca tccagcacat cgtcatcggt gtgggcatca
     3661 atgtgcgtaa gcaggaattt cccgaggaga tccgggaccg ggcggcagcc attgatgaac
     3721 aatgcggctt ccgcatttcc cgcagtcagc tgattgcgga tattatggaa gccttcgagg
     3781 aagattatga gatcttttta cagacccatg acctgaaagg tctgcgggcc tcctatgcgg
     3841 aacttctggt aaatcaggac agggaagtct gcgtgctgga tcccaagggc gaataccgtg
     3901 gcattgccag aggcatcaac gatcagggag agcttctggt ggagagaccg gacggcaccg
     3961 tggaggaagt ctatgccgga gaggtatcgg tccgcggaat ctatgggtat gtgtaggcag
     4021 gtacagcatt ttccgggatg gcagaaggca ggacggacag ccggatggaa agatcggaat
     4081 aaaaagaccg gaacagatag tccgtgacag aattgagaca gaacaggata tagaagtatg
     4141 gacgagaaca agatcgtact ctgtggcgcc aacgcctatg agaagaaata ttattttaac
     4201 gaagaacaat ttaagatgat tccggattcc atcaagcagg agctgcatgt gatctgcgtg
     4261 ctcttcaccg aggaagtggg cggtgtcttt accattgcct ttgaggagga tggaaatgtg
     4321 gtcatggaga ccaatgcgga tgatgatgac atctattatg atgagatctc ttccggactg
     4381 ttgatctctg agatccgtcg gagcagacag gagcttttcg agtccctgag tctgtattac
     4441 cgtgtgttta tcaaaaaaga agatgtatcc gcattgctgg atgaggagga cgatgaatga
     4501 tttttgtcat tgatgtaggc aacacgaata tgaccatggg tgtctttcag gggaaaaagc
     4561 tggaagccac cttccggatc atgacgaaga cccccagaac ctcagatgaa tacgggatca
     4621 tgatcaccca gctgctaaaa aataacggta tcgaagtgac ggacatcgag ggagccatca
     4681 ttgccagcgt agtgccagat gtgatgcatt ccctgaccag ttccatcatc cgttatctca
     4741 agacaaaacc actgatcgtg ggccccggtg tgaa
//
LOCUS       DAWDHD010000193         4773 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_1038_length_4773_cov_8.491098, whole genome shotgun sequence.
ACCESSION   DAWDHD010000193 DAWDHD010000000
VERSION     DAWDHD010000193.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4773)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4773)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4773
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1038_length_4773_cov_8.491098"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1491 a          898 c         1292 g         1092 t
ORIGIN      
        1 tcgatccgcg aattttcatg gtggcttcca tgtctgcacc gacagaggaa gagaagcgga
       61 aacctttcct gtatcgttat tttgtaaaaa ttccggaagc cgggaagttc gaatttctgg
      121 attccggctg gatggatgag gtgacgaagg gaagacttcg gggtgaattg ccggataagc
      181 agtacgcgga gcgtatcgag agcattaaac gttttgaacg acagttgacg gacaacggtt
      241 atctggtgtt gaaactcttt ttccagattg gaaaaaagga acagaagaag cggctggagg
      301 aactggaagg gaacaaggat accgcatggc gtgtgggcga gaacgactgg tggcagaaca
      361 agcattatga caaatgtgag gaagtcttcg acaaatatct cacagatact aacgcttccg
      421 tagctccgtg gtacattatc gactccgggg acaaaaagtg ggcggaatta caagtgctgg
      481 agacattatg cagcggtatc catgtggcca tgcagaatga gagccttgct gtgcctattt
      541 tgcagaatgt attcccactg gtgaagatgc cgaggctgtc cgaggtggaa ctggacaagg
      601 aaatctccga ggaagaatac aagaaagaac tgcgtcatct gcagaagaag ctgaatgccc
      661 tgcattaccg cctttatcgg aaaaagattc cggtagtcat cgcctacgaa ggctgggatg
      721 ccgcaggcaa gggcggcaat atcaagcgga tcgcgggagc actggatccc agaggctacg
      781 aggtacaccc catcgccagc ccggaacccc acgagaaagc gagacattat ctttggcgat
      841 tctggacgag acttcctaag accggacata ttgccatttt tgatcgtacc tggtacggac
      901 gcgtcatggt agaacggctg gaaggcttct gtagtaccaa cgactggcag cgggcttaca
      961 atgagatcaa tgagttcgag aaggaattgt cccagtgggg tgcggtgatc atcaaattct
     1021 gggtgcagat cgacaaggac acccagctgg aacgctttac ggaacggcag aacaccccgg
     1081 agaagcagtg gaagatcacg gacgaagact ggcgcaaccg ggataaatgg gatcagtatg
     1141 aagatgccgt aaacgaaatg ctccagaaga ccagcacgga gtatgcaccc tggcatattc
     1201 tggaatccgt agacaaaaaa tatgcccgga tcaaagcatt gaagattgtc attgccgaac
     1261 tggaaaaggc tctgggatag gaaaagaaag tagagaacgt aaacaccgtc ttgtaaaaag
     1321 gcggtgtttc tatatgtgat gaaaccgttg acaatgtaag attcataaaa tataattatt
     1381 tcatcgaaac ggtgaaaagg ggtgaattga tgaaacaaaa tttatcacaa atacaatatg
     1441 agcaactggc cagagaacaa ttttgcaacc ggctgaaaga aattccgttt gtttcggata
     1501 tcgaggtgtt tttggaaaag aagaatgcac cgttggggga tttcaaggca aaggtgtatt
     1561 tggaggacag cgatgagcct cttttatttg tgattgaagt caggtctaac ggagaaaaga
     1621 gattcgttaa tcattttatg gaaatcgtga tgcggcagga tgataaaacg cattatattt
     1681 ttatggcacc atatatttcg gagcagtcag ccagagcaat gaaagaggaa aaatgcagtt
     1741 atatggatct gtccggaaat tgttatatct taacgaaaag atttctgctc tatgtcagtg
     1801 gacaggataa caagttcata acccacaggg aaaagaaaaa ttacttatcc aaatcgtcaa
     1861 gtgctgcatc agcaattatt cgtaccatgc tgaatgaacc ggagcgaaaa tggcaggtga
     1921 aaacgctggc tcaggagagc aatacggcat tgggaacagt atctaatgtg aaaagttttt
     1981 tgcttgccag agactggata gataagagta ctacggaatt ccgtttgcag aacattaggg
     2041 aactgtttta tacatgggca aaggattatc atcaaaaaga agcgagaaaa ttacagtttt
     2101 attctctgga tacggttccg gagatagaac aatcagccgc tgaatggagt aaaacacatg
     2161 ataatggagc tatattaggt ggcttttcgg ctgcggcacg atatgcgccg acagtacgtt
     2221 atcaaaaagc cgaaatatat gtagaacctc aatttgttca ggaatttgta aaagatctgg
     2281 aattgcagcc ggtcaacacc ggaggaaatg ttgtgataac gataccgcat gacgaaaacc
     2341 cctgtatgta cgcaaagcct gttcatgata cattagtaac ttctccggca caaacggtaa
     2401 ttgatctgct gggggatgcc ggaagagggg aagaggctgc ggaggcaatc ctgcgcagag
     2461 aatacccgga gaggacagag gatgaaagaa gaactgaaaa aggcaactga ctattcagag
     2521 ggacaaaaga cagccgcata catggtactc ggagagatcg taaatctttt gaatgagttt
     2581 tcagataata tcagaatcat aggaggatgg gttccgacac ttttataccc ggacggtgac
     2641 catataggtt ctattgatgt agatgttctg ctgaatcaac tggagatcaa aaaggcagaa
     2701 agctatcgga cgattaaacg gatactgaca caaaatgagt atagaagaca tgaagaaaaa
     2761 tattttacat ttataaaaac aatagtttta gatggagttt cctatgacgt agacgtggat
     2821 ttcctgtccg gaaaatatgg cggcgatggc ggaaatgtaa gtaaacatgt ggatggtatt
     2881 aaaaccttac cggctacggg aggaaatttt gcatttgatt ttccgccaac ggatgtgaaa
     2941 atagaatata tgcgggagga tggagcaaag gacagtggac atgtgaaagt gatttctgtg
     3001 gtgccttatc tggtcatgaa gacagcagca ctggggaggg gaaaaccgaa ggatgcgtat
     3061 gatatatatt acatggttga caaatatgag ggaggcgtaa gaacacttgt taaggaattt
     3121 atgccttata cagacagaga gttgatcaga gaaatgtgtc agaagctgga tgaaaaattt
     3181 gcttcgccac agcatgccgg accaacagat gtagtgacat tcataggaat aacagaggaa
     3241 gaagaaaaag aacagatccg acaggatgtt taccagaaga tacactattt aacaaataaa
     3301 ttgaaaacaa tgtaagcaaa atcttgtgtt ggcagacagg tctgttataa tgatacatag
     3361 ttgtatttcc cagatagtat ttacagaacg agaggagaaa tcatgggcga gaaccgtaag
     3421 atatatgcag cattattggg agcaggaacc gttggaagcg gtgtgtacaa gctggcaggg
     3481 atgcaggaag aggatatctg cctgaagaca ggagcacatc tggagattaa aaagattctg
     3541 gtgcgcaatc ccggaaaggt aagagaagga atcccacagg agttgttcac agatagctgg
     3601 caggagattt tagaagatcc cgagattcag atcgtcatcg aagtcatggg cggcatcgaa
     3661 ccggccagaa cctatgtgga agaagccatg aaggccggca aacaggtggt caccgccaac
     3721 aaggatctgc tggcggagta cggagatcca ttatttgaga ttgctacaga gaataaatgc
     3781 gacttacaat tcgaggcagc tgtagcagga gccattccca ttatccgacc tttgcgtcag
     3841 agtctggcgg gaagtcaatt gacggaaatc atgggaatcg tcaacggtac gacaaactat
     3901 attttgacca aaatgtccga ggaaggcatg gggtatcagg aagcattgga tatggctatg
     3961 agactgggct atgccgaagc agatcccacg gcggatgtgg aaggctatga tgcgggacgc
     4021 aagatcgcca ttatggcatc catcgctttc agctcccgcg tgacgttccc tcaggtttat
     4081 acggaaggca tcacgaagat tacagcagag gatatccggt atgcgaaaga attcggctat
     4141 gtgattaaac tgttgggtat tacaaagctt acaggtaaag gaattgaagt aaaagtacat
     4201 cccacattga tcccggacag ccatcctctt gccacggtca gagattcctt caatgccgta
     4261 tttgttcatg gtcaggcttg tgatgatgcc atgttcatgg gacggggcgc cggacagatg
     4321 cccactgcca gtgcggtatt gggagatgtc atagatgtga tgcgtaatat tatccatgga
     4381 agttgcggca aaattggcag tgacagccac aagaaacttc ccgtactgga tgtggaggag
     4441 acgaatagcc gtttcttcct gcgcctgcag gtagagaacc gtgccggagc attggccaat
     4501 atcgccggag ttcttggtaa caacgatgtc agcatcgccc agatggtaca gaaacgtagc
     4561 cacgacggta ttgccgagat cgtggtcatc accgatcttg tgaccgagag acatttcaac
     4621 gacgccatcc gcattatgga gggaatgtcc gtcgtgaggg aaatttccgg cattatccgt
     4681 gtgtactaaa agtatgataa attgagagtt agtgaaacaa atccacgaac gcccatggcg
     4741 gacggaatgt ccttcctcga tgtaaggtct ccc
//
LOCUS       DAWDHD010000194         4765 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_1039_length_4765_cov_13.602548, whole genome shotgun sequence.
ACCESSION   DAWDHD010000194 DAWDHD010000000
VERSION     DAWDHD010000194.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4765)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4765)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4765
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1039_length_4765_cov_13.602548"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1386 a         1055 c         1291 g         1033 t
ORIGIN      
        1 gcttcgagga tgcacaggca gccagagcgg cgaaccagcc tctgttccca aggaattacg
       61 gaggccgggg aggcagccgc ggcggatcct atggaaatgg gaacggcaca ggggtacagc
      121 agaaaaaagg catgcccgcg ggggcctgga tccagtttcc caatatggaa gcctataagg
      181 caagagaaca ggaattgcta caggccatcg ctgactccga cggcaatgac gatgtggtga
      241 tctacctgag ggatattaaa aacatcaaga tcctgcctgc aaatctcagg gtaaaagccg
      301 atgagacatt aatggaaaag cttgcatcgc agtttggaaa agaaaatgtt aaatttataa
      361 cgaaacctat tgaaaaccgc tcaaaaatag attaaaataa ggaatcgaga agagaaaact
      421 attcgttcgt ttacttagga ggtaacagtc atggcaaaag agattaagac gatcggcgta
      481 ttgaccagcg gcggtgatgc accgggaatg aacgctgcta ttcgtgcagt tgttcgtaca
      541 gccattgcaa gaggactgaa ggttaaggga atcaaaaaag gctatcaggg tcttttgaat
      601 gaagagatca tagatatgga agccaaggac gtatccgata tcattcagcg cggtggtacg
      661 atcttaggaa ccgcacgttg tctggagttc aaggaagcgg aaggacagaa gaaaggtgct
      721 gagatctgca gaaagcacgg tatcgacggt atcgttgtca tcggcggtga cggttcttac
      781 cgtggtgcac aggcactgtc cagacttggt attaatacca tcggtcttcc cggaaccatc
      841 gacctggata ttgcatgtac cgagtacaca atcggtttcg atactgctgt caacacagca
      901 atgcaggcaa tcgacaaggt taaggatact tcttcctctc atgagagatg cagtatcatc
      961 gaggtaatgg gcagaaatgc cggttatatc gctctgtggt gcggtattgc caacggtgcc
     1021 gaggatatcc tgcttcccga gaagtacgat tacaacgaac agaatattat caataatatc
     1081 attaccaacc gtaagcatgg taagacccat catatcatca tcaatgccga gggaatcgga
     1141 cattccactt ccatggcccg tcgtatcgag gctgctaccg gtattgagac ccgtgctacg
     1201 atcttaggtt acatgcagcg tggcggaagc cctactgcca aggatcgtta ctatgcatct
     1261 atcatgggtg cttacgcagt agatatcatg ctggaaggca agaccaaccg tgtcgtaggt
     1321 tatcagcacg gtgagttcat cgatttcgat attgaggatg cactgcagat ggagaagggt
     1381 atcaacgatt atcagtttga ggtatctcat ctgctgtcca tctaatttaa tttaacagtt
     1441 cagactgcga agcaggcact tttataaata agagtgtctg aacggatatg aagacaaagg
     1501 cgtttcccat tgcgtggaaa cgcctttttg actataataa actcatccat agaatgcatc
     1561 ccgactgctt gcaggcaggg gatcattcta tgagaggagt aaacaaacat aagtaagtag
     1621 ggcgattagc ccggattacg catgtttgtt atggtttcga gagttcgcag aacggagaaa
     1681 ccatattatt atgatgttta ggagcggtta gcagacagat atgatcacaa gcaacaacaa
     1741 tgcaaaagta aaacaggtgg tgcagtggca gaccaaggca aaggagcgcc gcaaggatca
     1801 cgtatttctg gcagaaggta tcagaatgtg tgaggaggct ccggtaaaaa acgtgcggga
     1861 ggtctacctt acggaagaac tggagcaaaa gatccggcag aatgcccaga acggagacac
     1921 agcattctgg gacaagctgc agcagaccgg ttacgagacc gtctccacgg aagtctttgc
     1981 caaaatggcg gatacccaga ccccccaggg cattctgaca gtgctggaac agccggtcta
     2041 tgatctgaca cagctgttgg aacagccgga tcctttattt ctgatgctgg aaaatctgca
     2101 ggatccgggc aatctgggaa ccatgatccg taccggagaa ggagcaggga taacgggtgt
     2161 tatcatgaac aaccagacgg ttgatatctt taatcccaag accatccgtg ccaccatggg
     2221 gtctattttc cgggtaccct ttgtctacgt gccagatctg gcaccggtgc tggaacagat
     2281 gcatgcaaaa ggtatccaca cctatgcggc acacttgaaa ggacaggaat actacgattc
     2341 cttttctttc cgggagccta cggcgttcct catcggcaac gaaggaaacg gcttatccaa
     2401 ggaaatctcc gaccaggcgg gacagtatct caagatcccc atggagggca gggtggaatc
     2461 cctgaatgcc tccattgcag cggcccttct gatgtacgag gcccacagac agcgaagcgc
     2521 aagactgtaa ttatcccgga cccatttgcc ttatagatat tatcaaatgc caggcattcg
     2581 atgcgcagcc gataatattt acatggctct gaataattat gatgtcgggg tcaaaagccc
     2641 cctacaccat aataatagaa gaaagacaga acaggaggaa agatatcatg aagatcggat
     2701 ttatcggact gggaaatatg gccactgcca tgatcggagg aatgttgcaa aaaggaattg
     2761 cagctccgga ggacatcatt ggttcagcca gaacggaaac aacagtggaa aaagttaaaa
     2821 caaaatttaa tattaacaca acaacggata acaaaatcgt agcggcacag gcagacattc
     2881 tgttcctggc agtgaaaccc atctttttcc cggaggtgat cgaccagatc aaagatgtcg
     2941 tggcagagga taccctgatc gtcagcattg cggcaggaag aactctggac tatctgaaag
     3001 aggctttcgg cagaccggag ttgaagctga tccgctgcat gcccaatact ccggcactgg
     3061 tactggaagg ctgcaccggc gtctgtgcgg aagaaaacgt cacggaggaa gacatggagc
     3121 aggtactggc actgttacgt tccttcggca ttgccagtgt ggtaccggaa cggctgatgg
     3181 atgtagtcgt aggtgtcagc ggtagctccc cggcctatgt gttcatgttc attgaagcca
     3241 tggcagacga ggcagtagcc gccggaatgc ccagaaaaca ggcttatgaa ttcgcagcac
     3301 aatccgtcct tggcagtgcc aggatggtgc tggagaccgg cagacatccc ggagaactga
     3361 aggacatggt gtgctccccc ggtggaacca cgatccaggc agtcaaggta ctggaggaaa
     3421 aaggcatgcg cgccgctgtc atggatgcca tggatgcctg cattgagaag tccaaaagca
     3481 tgtagtgaca gctcgactct gttcatgagt tgagctgtca tggctaaata gcgcccaaag
     3541 tcccaaaact tgacaaaaat tttgtcatct gttatgatat ctcgcgtaac aaatttaata
     3601 tttttgtaac aaaccagtga aaatcttaat aatttcagca tatactattc actggtgaga
     3661 tccacaggta tcacacccat cggggaatct tcctgtggga aaggcgtgta tcaagatgac
     3721 aaaaagcaga agaatgctgg ttgtatgtgc aggactgctc tgttctatct tcctgagcat
     3781 gcaaatgagc atgaccgcac aggcaagcaa tactaaaaac tatgtcaatg atctgaacgg
     3841 tggtgtcgca tcgatcttag accccggttc caaaaacagt accgaggtca tcaatgcaac
     3901 ggtgaaggaa ttaaatctga cattccccgc tgaggaagag atcagttccg ggctggtcat
     3961 ggcaaacgta aaagatgccg tgaatgtacg aagcgatgcc aatgaggatg cttccaaggt
     4021 cggcaagctg tacaaagact gcggaggtac gattttagag cgcagggacg gttggaccaa
     4081 gatccagtcc ggtacgctga tcggctgggc caaagacgaa tatctcctgt tcggggatga
     4141 tgcgaaggca ctggccaatg atgtgggcag aatgatcgca cagatcaaca cggagactct
     4201 tcgtgtgaga accgaggcgg atcaggatgc cggagtcctg ggacttcttc ccaagggcga
     4261 tatagtggat gtggtagata acagtaatcc cgaatgggtc tgcatcgact acgaaggagc
     4321 agacggatat gtgtctgcgg agtatgtaac agtggatttc cagatcgact ccggtgagac
     4381 tttagaggag atcaaggcaa gagaagctgc agagcgtgaa gcaaaacgcc atgtgaacta
     4441 cggcgaatac accaccgatg cggataccac gcagctgttg gcagcattga tccagtgcga
     4501 ggcaggatgc gagagctacg aaggacagct agcagtaggt gctgtcgtta tgaaccgtgt
     4561 acgaagcggt gcttacccga gtagcattca tggtgtgatc tatgcgtccg gacagttcac
     4621 tccggcattg aacggtaagg taaataccgt atacgaatcc ggcaagatca acgcaagctg
     4681 catcaaggca gcggaagaag ccatctccgg cgtatccaat gtgggagatt tgacacactt
     4741 cagacgtaac aacggccgtg acgga
//
LOCUS       DAWDHD010000195         4738 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_1046_length_4738_cov_14.493274, whole genome shotgun sequence.
ACCESSION   DAWDHD010000195 DAWDHD010000000
VERSION     DAWDHD010000195.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4738)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4738)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4738
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1046_length_4738_cov_14.493274"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1241 a         1091 c         1061 g         1345 t
ORIGIN      
        1 cagaagagat ttgtcatctg aatcggaata tgtatttgaa cgaaaatgaa cagacgaaaa
       61 atattatccg gagcacaatt aaagtatatc gcctttacgt ctatgctcat agatcatgtc
      121 aataaggctt tgatttatcc taatttaaat ggaggaatat tgcttgtgat aagtgatatt
      181 tttgacgtat taggaagaat agctttcccg atttttatgt ttttattgat agaaggttat
      241 tttagaacca caaacagatg gaaatattta ggaatagaaa aaggatagcg taaccgcatg
      301 gcgggaagcg ctatcctttt tcctatctga ttgcaatttt tccttcaaga tatcttttat
      361 ttacatactt tttctgtcgg caatctctgc catcttcgca gcattcagtc tggtatcacc
      421 atgcactctg gaataggaca gatatccatt catccggtcg atcttggtca gattcttgct
      481 gccgcatttc ggacacacat ccatctgcag ctcctcgtgt ccgcagtcat cacagtagga
      541 cagtgagaga tttaccccct catagaagcc catatccatg gcgcggcgga ttaaggattt
      601 gatcgccagc gtgttatagc caatcggata tttcacatac tggatcttgc cgccgttact
      661 taagttccag aaacggttct ccagatcctg cttctggatc ggagtgatgt cctcggagac
      721 atggcagtgg aagctgttgc tgacataagg tctgtcagat acgccttcga tgataccgta
      781 ctgctctcta aactgtctaa tctgcagtcc gcagaggttc tcagcaggtg ttccatagat
      841 ggcatagaga ttgccatctt cttccttgaa ctcatccacg cgcttgttga tatgttccat
      901 gacctccagt gcaaaagcac cgtcttccac aagtgatttc ccattgtaga gcatctgcag
      961 ctcattcaga gcagtgatcc cgaagctggc agttgctgtc ttcaggacgg gctttatttt
     1021 gtccgtaagc ttcagatgtc caccgtaaaa tccaccctca caatatgcta aaggattcgt
     1081 ggatgcacgc atctccccta aataagcata cgtccggata tggagcctac ggatcagctc
     1141 cagatagtaa tccagtactt cgtagaagtc tctgctctct tttcttgctt ttgccagtat
     1201 catcggcaga tgcagggata ccacgccaat gttgaagcgt cccacaaata ccggttcgtc
     1261 tccatcaaac gccgggtgca taccgccttg ttcgtaccag ggagacagaa atgctctgca
     1321 gcccatgggg gagatgatct taccgtattt cttgtacata cttgcgacat agccctttcc
     1381 ggtcaggctc agccagtcgg gatacatggt cttggcagaa catgcaacac ctgcttcaaa
     1441 cacgtcttct aaaggtttac cggggccatg gaggttctca tcgtataaaa atacgatctt
     1501 cggaaacagc accggcttct tatgtccggt ttttccctgg ccgccccttc ggacttccag
     1561 catggcaacg gaagccattc tggcaaactg tccggtgccg gttccggctg tcacggtaat
     1621 gaaaggatag tccccacggc tgcttgccac ggtgttgaat ttatattccc agccctggaa
     1681 gccctgctcg aactccttct tcacatccgc aagagcttct ttttcggcaa gatctgcagc
     1741 gacacccagc cgctcatact tagccagggc tctgcggtag gatttctcgg cataaggctc
     1801 taagatcaga tccacactgg ggacagtaaa gccgccgtac tgctggctgg cagcacttaa
     1861 aacgatatcc ccgatgacat cgaatgcaac atccagagtc ttcggctcat tgtaccagat
     1921 attccccatc tcgaagccgc cggagagcac atgcgccaca tcgaacagac agcagttcat
     1981 ggtatccctt ctggcagaca tatcatggat gtagatatac ccgtccctgc atgcctggat
     2041 ctcttctgtc gtcaggaaga acttctggta aagctctttg tttaactgat taaagatcag
     2101 gctgcgcttt gtggagacca gtgcggaatc tgcattggaa ttttccttat cgcccacata
     2161 catgatcgcc tggctttttt tgtagacatc gtccagcatc cggacaaagt cctgcttata
     2221 gttcctataa tcgcggtagc ttttggctac gatgggacgt acctgctcga gggcattttc
     2281 cacgatattg tgcatgacag agatgggtac ctgtgtggat tccagagcat ctatatgaga
     2341 aatgacgcat tcacagatct gttccttttc ttccggagtg aacttcgtaa gtgcgcgata
     2401 agcacttttt ccgatagcat cgatcacctt ctggacatga aatgcctcaa gactcccatc
     2461 ctttttgatc acataggtct ctctttccgg agaataacgt tcgttgtagt tttctacgtt
     2521 gttagtattc ataggctcac gcttcccata gaccgtggag gttacaataa gcatacacgg
     2581 cttccacagt ttccccttcg cagagggcaa attctgctct cggagccata tccggggtca
     2641 gattcttata ctgcacaccg cggtctgtct gcacggcgat cagggagata tagtgctccg
     2701 gggtcatcgg atgctccacg gagccgacat gtacggagac aatcccgttt tttaccgtat
     2761 attcgggaag atgcttctcc actgcggcat ccacggtgcc gggtaccagt tcggtcatct
     2821 tttccccgca gcagaacagg ggaaccttat gatctacaac ctttatagca acatttttac
     2881 aatgatcaca aaaataaaat ttaatttcca tagcttttct cctttgccta ataaaaaata
     2941 tttattttga cacttatagt ataacttgtt tcgaccggaa atgatataga ccgggatgta
     3001 gaatatgata atgagaatta ttattataat tgttaataat taccaagatt caccagaaaa
     3061 tggcagattt caaagtgatt tgtaagtaca aaaagaatat taacgtaaat tttatataaa
     3121 ttatggaaaa gctttttgca aaaaattcag ataagatata gtgtagagcg atagatgttt
     3181 ttaagacttt tatgttttgt ttgcattaat gttcaactgc tatgattaag ttgtcagttc
     3241 catcggaaga tcaaaaggta cttggcacgc agaagaactg taagaggatg catttcatgc
     3301 tgtatgaaga gggagcgaca gcatccataa agtcagtggg cgaggaccac aggaggtagc
     3361 aattgagaac acagatcatc aatgatttaa tgaccaatat ttttttcaat ctgatcgaga
     3421 cagaggacag gattgcagcg gatgagcggt ttgaaggttt aagcagcaat gatgccctgg
     3481 agatctataa ggtgctatct gaccagctga tccgcaccca tatttatgcc gcacatatcg
     3541 gaagcgggat cagcagcaga atcgcaatgt ccatactgtc gtccgggagt ttcctcttat
     3601 agaagatctt tccgtctccc ctgaaggcat aacgatattg gatacggtgt atgtgccgcc
     3661 attaatgtaa aaggttgctt tcgccggatt tttcgggttt acttcggtgg cactggttaa
     3721 cataatgctg gaaataacat ccgtatccac acggtacgta tagtccgggg cttccagctc
     3781 ttcggaaggc acatctttgt agcttacaat gccgatgccc agggaagaga taatggcatc
     3841 atccgacact tttccagagg tgcttccagt ccatgcaggg tatccgagat cagatttttc
     3901 taaaaacaac gcgagtggca ggttctggtg cgtcagggag ggaagcaccc ttcttaatgc
     3961 acctccggat aacttgttgt aaagggcagc ctccgttgct gtcatggcat agttttttcc
     4021 attgtgcgtg aagtaggcaa tgggttcgat caccaatttg taatccccat taatcagatt
     4081 ctcaaagggg atgccggttt tggatgcgat aaatttagct gcatactctg agcagatgta
     4141 gcgttttatc gcagcaatgc tggctttgtt cacagatccg ctgatgattc ttggtagagc
     4201 gacggctggc tttgagtagg tgtaggaggc attaatggaa agcgatgagc ccgcagtata
     4261 ttgcagtttg gacacttttc caaagtttaa caccgtggag gaaatgttgc agtttgataa
     4321 atcaatggga gtagcaacaa cgctgcctcc cgtggtaacg actgtaaccc gaacaccatc
     4381 ctggttattt cgccagacgt ttagttcgga tcctgtacca aaagatccac cgcctccatc
     4441 aatatttcca tcacccatag cataagtaga aatgcctgtg gataaaaata tcgttaggga
     4501 tagaagcagt ggaaaaaagt atttcctgat tgctctcata agatctcctt tgcaataaaa
     4561 aagacatggc gtgcttacca tgtcttagtt gttttgttat tctcccatag tgccgatctg
     4621 cttgttgatg tcgccatcac tgtcaaccac atcataagta gtatttccgg tatcaatcca
     4681 accaaatacc ggatcataga cttgtccggg atgggaactt tctgaattat cagcagga
//
LOCUS       DAWDHD010000196         4636 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_1074_length_4636_cov_14.080114, whole genome shotgun sequence.
ACCESSION   DAWDHD010000196 DAWDHD010000000
VERSION     DAWDHD010000196.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4636)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4636)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4636
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1074_length_4636_cov_14.080114"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1038 a         1289 c          930 g         1379 t
ORIGIN      
        1 taatcataca cccggtaggt aatatcgctg ctctgctggg tctcaagaag cagtgtaccg
       61 cccttaatgg catgtacggt accgggatcg atctggatga agtccccggg cttaatagga
      121 atctcacgga tgaactcttt ccactttcct tcctggatca tctgctccag ttcttccctg
      181 ctcttcgcgt tgtgtccaac caccagcgta gcattttcct tacagtctaa gatgaaccag
      241 cactcggttt tgcccaggga tccgttctca tgtaccttcg cataagcgtc atccggatgc
      301 acctggatgc ttaagtcgtc ctttgcatca atgatcttga tcagcagcgg gaaacgatcc
      361 attcccgtgt ctccgaacaa ctcggggtgc ttctcccaca gctcggacag cttcattccc
      421 ttataagcgc cgcttgccac ggtgccgtct ccgttgggat gggcgctgat accccagcac
      481 tcaccgatgt cgtcgccctc gatggggtac ccaaattctc tgtttaaccg ggtaccgccc
      541 cagatattgt gggtacacac cggtgttaaa aaaataatat ctttgttcat gatcatctat
      601 cctttcccat gatacttact atgtattgca cggcatcttt cctgttattc cgctacttag
      661 ctttgcaacg tccgtttctg gaaaaatatt ctatctgata tgtaacacat ttcttccgat
      721 ctgtctacct gacttccgta ctatgcctat tccgcttctg tttctgttct taccccggat
      781 acaatcaatg acaccagata aaatccagcc ttatcgttct cgtgtgtctc cgtgatctcg
      841 attctcaccc ggtgttcccc atagggcaga tgctccgcaa tcgttgccag ttccatctta
      901 tctccccagg tctcatcaaa ggttccgtcc aacacgcacg ccgtctcagg gttctcatcc
      961 accactgctg tggcaatggg tgcaggtccg tccttcactc ggcggtattg cacggcggca
     1021 ccggttcccc ggaaagtgaa ctccagataa gctccttttt ctccggcgct ccatccgtta
     1081 cggaaaatat ccgtgatgtc tctctgcggt gtcatatctt cttcaaaacc gctcttctcc
     1141 ggtgtggaat taaaatattg tagtctcacg caatgctcat aggcattctc tgtcagcggg
     1201 gccgggaaaa tttcctgcgg ctcttgcgtt ttatcatgga ttaccttttc caggaaattc
     1261 gtgatcacag atgccatgaa ttcatgacca agatcattgg gatgaaggaa atctgccgta
     1321 atcttctctg ccggcagtct tccggctgcc acagcgggat agatactgtt ctgcatactg
     1381 atgcagggca gatcataatg tcgtcctacc tgcgcatgat aatatgccgc acttttaccg
     1441 gtatcataat agacattatg gatcagcacc attgccggtg cgctcgcact gccataaatc
     1501 tgcctcacca gaccttcgta ggtctcacag aaatgcggcg tactgtgatc gttcacggaa
     1561 aattctacaa ataccagatc cggtagccgc tgaagcaggt cttcctgtac tctcgccgcg
     1621 ccgaactggg aatcggtagc tccgattccg gcgttcacat aatcgaattc cgtattgggg
     1681 aacactttcc gaagccatgc aacggttctt gcagcatagc attgatccgg ctccgtcgct
     1741 gcgaatccct gcgtgatgga tccgccgatg aatcccaccg tcagtctctc ccctttggtt
     1801 gcacggttca ggagattttt cagtctggtc agattaccgc ggtttaacat accggcctct
     1861 atatcaataa catttcccgc actcttgcgg ttctcgtaaa tactgttttc cacggtgcaa
     1921 taacgctttg ccttgttttt caatgtcttg ctgatccgga aaatctcttc cggacggtat
     1981 cccctggatt ccagataata aataccaccg taaagcttga tgggaacatc cggatattta
     2041 ttcccgatca tggcttcaaa catgatcata atgtgaccga tcttctccgg tgctgcttcc
     2101 acatgattgt ccaatagcaa taggaactca tcctttttca aatgacacac catttcctgt
     2161 cctttcaggt gcttttgaat atgtgtcccc agatggtaca gaaggctgtt tttcacttcc
     2221 tcactcatct tcgcggaaat ctcatcgtaa tcccggatgc ccagaaatac ctctgcatac
     2281 tgaccgtcct gccagggcag gatctgttcc agttttatcg aaaagctttc cgttgtctcc
     2341 attccggcaa tgtccggttc cggcttttct gtatcctttt cccgctccct gacattccct
     2401 gcatattctt cacggttcag atagctttcc atcatccgcg tcatagtaga cagaatttca
     2461 ctttccgttt ctgtccactg acgtccttta taatatttct ccagctcctg ccagagaccg
     2521 gtctcccggt ctgttccttt ccgttctgaa gtataatcga cataattata gttcttgtcg
     2581 tacacgctga atgcattttt gccgcttcgt tttacccggt acaacgcctg atccgctttt
     2641 tgatacagtt cctcataaga ggaagcatcc tccggatatc gggagattcc catacttaaa
     2701 gtcactcgtc tgcctgacag ctggatctga tccgccatac gggatattct ctctgctgtc
     2761 tccacagcat gctgtcccac atttttccca tggcagaata cacaaaactc gtctccaccg
     2821 atgcggcccg cataatcctc catgcctgca ctgatcaaaa tcgtattggc aattttgatc
     2881 aatacttcat caccgtataa atgtccgcaa gtgtcattga tcgttttaaa gtcatcaata
     2941 tcaacgatga taaaagctcc ttctgtgtca tcggcctgcc ggatatgctt ttccaccagc
     3001 ttacgcattt tcgctttatt gtacagatct gtcagggagt ccctctctgc cttctgttcc
     3061 aacagctcct ggttcttttt ttctcctgta atatcaagcg tcttccccat gatcttatat
     3121 ggcttatgat ccttatcata gaccggaatc cctacataac ggaaccagac aaataccgat
     3181 tcatcactga cttgtctgac ttcataggaa atatgctcat cgccccggtc cattgcggcg
     3241 cagaattcat caaaagtcgc ccaatcatcc ggatgcacaa atccccagtt atgcatctgt
     3301 tcctgatagt tttcgatgac catgttggtg gtattccact ttcctccaag ctttctggaa
     3361 agcacatatt tttttgaagc aatttcatac tcccacagcg cacagtcgga attgtcctcg
     3421 gtaatcttcc tcctgatacg ttcttctgcc agttctcttt ccagttcttc gattctggca
     3481 tataattcct ctttttccat catacgctgt ctccatcact taaaaaggac ccgctcatcg
     3541 cgggtccctc gattctatct tctgccttct tctgtatcgc ttgttgtgac cacgctgtgt
     3601 tctttgatat agtcaaccac ttcatcgatg gtcgtaaatg ccagccctac gtagctgtct
     3661 gctgcgccat agtaaatggc gatcctgccg ctctcggaat catggatggt agcgcagggg
     3721 aagcatacat tcggtacaaa ccctctctcc tcataccact cttccggtgt cagcaggaag
     3781 ttctcacaac gatattttac gatggacggg ttgtcaatat ccaggatcgc tccaccgatg
     3841 gaatagacat aaccgttaca ggttccgctc acaccatgat agaacaatag ccacccttcg
     3901 gaagtctcaa tgggagctgc accgccaccg atctttaagg actcccacca ttcggaagac
     3961 ttgcccatca catgtctgtg tttgccccag aataccatat cggggctctc actgacgaaa
     4021 atatcaccga agggcgtatg tccgctgtcg gaagggcggg acagcatcat gaaattgccg
     4081 tgtacttttc tggggaacag tacggcgtta cggttaaaag gaaggaacgg attctcgatt
     4141 ctggtaaacg tcttgaaatc cttggttttc gcaataccga tggctgcgcc gtagaaatcc
     4201 tgacaccaga tgatatagta ggtatcttct acctttacca gtctgggatc atatgcataa
     4261 atcggcatga aaggcttgcc ttcttcatct acgaactgga tcttattctt atcaaaatcc
     4321 cagtgaatag catccttgct gcgtcctaaa taaatatagg gaataccgtt gacctgttca
     4381 ccgcggaaca cgccgatgaa ttcatcaccg taaggcatta ccgcactgtt gaagattctg
     4441 gctactcctt ctacaggatt tctgccaata atagggttct cactgtatct ccataccggt
     4501 gcaccggtat gtccttccgg tttatcctgc cagggtacat taggtactgc agggctgatc
     4561 atcttaactt tgctcattcg agttacctca tttctgtatt atttatcttg cttcctgttt
     4621 taagaactct tcattt
//
LOCUS       DAWDHD010000197         4617 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_1082_length_4617_cov_9.994301, whole genome shotgun sequence.
ACCESSION   DAWDHD010000197 DAWDHD010000000
VERSION     DAWDHD010000197.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4617)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4617)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4617
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1082_length_4617_cov_9.994301"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1171 a         1232 c          978 g         1236 t
ORIGIN      
        1 attccgtatc attcatattc cagggaatat tttcctcaag ggctccggcg ggctgtactt
       61 gtttgcgaac gcagataaca tcctgaacat ccagattgta aattttacag ctgccattgt
      121 cacaggctct tcccgtactg tcaagtccac aacccaaatt gaccaccgcc gcatcaggat
      181 ggtctgccag ataattttgt acctcccacg caaggtcatt ctgccgcatg gcaacctcca
      241 gcgaaccgaa acgctgcatc aggctgtgag attttttctc cacctgtgaa aagtcatagt
      301 cgatctggtc gatcaggtgt accgccatcg cgtcccgata aaggttcgga tacaactccg
      361 agcatagctt tcgggaataa agcgggatga tcagtgtctc ctgtacggtg ttcttctcaa
      421 tcttgtattt catggacatc tctcactttc ttgcattttt gccatttcct ttttgttagt
      481 tttgtttaac cgctgtatag agttatgcgg ttagtttgtg ctaacttaaa tatcttagaa
      541 aagccgcatt actgcggcct ttcagctgac tgttttggtt ttcgtttata ttatactgaa
      601 cttatttctg ttttgcaagg tagatttcca ccaatagata tttcttgtct tcttcccagt
      661 tatagtagaa ctgaatcagt ccgatgcggc tcctggtgcc ggtcttctca ttcaacgcag
      721 aaaacatgcc gctagagcag cgcacgggag acaaataagg ctgtcggcga tcaccggcac
      781 cgacaagaaa aatcccactg ccttgaacgc agtgggattc ccggttttgc ttttccataa
      841 tagaaaacag ctaatgaaag catggtctct ccttaaacag actggaaacc gccttccaag
      901 tcttcaataa tatcatcgat attctctgta ccgatggaca gacgaatggt attgctgtaa
      961 ataccctgtt cgttcagttc ttcctcagac aactgtgcat gcgtggtgga agccggatgg
     1021 atgaccagag acttcacatc tgccacgttt gccagcagtg agaacagctc cagattgtcg
     1081 cagaactttt tcgctgtctc cttgccgccc ttgatctcga aggtgaagat ggaaccgcct
     1141 ccgttcggga aatatttttt atacaactcc tgctgctccg gatcggtgga tacggaagga
     1201 tgatgcacac gctctacctg aggatgattg ttcagatact gtaccacctt cagtgcattc
     1261 tccacatgac gctccacgcg cagagacagt gtctccagtc cctgtaagaa aataaaagca
     1321 tggacagggg aaatgcatcc gcccatatca cgcagaagca ttgcacggat tgctgtcaca
     1381 aaagctgcac ccggtactgc cttggtgaag gtaacgccat gatagctggg gttcggagtg
     1441 cacagatgag ggaacttgcc gctgccctcc caatcaaata taccattttc cacgatcaca
     1501 ccgcctaagc tggtgccgtg tccgccgatg aatttcgtag cggaatgtac tacgatatct
     1561 gcaccgtact caaaaggacg taccagataa ggagtagcaa aagtattgtc cactaccaga
     1621 ggcaccttgt gtgcatgggc gatctgtgcc agacgctcga tgtctaccac atcagagttg
     1681 gggttgccca gagtctcgat gtagagagcc ttggtgttct cctgaaagct tgcttcgatc
     1741 ttatcataat cggaaggagc cacgaaagtg gtggtcacac cgtagtccac cagagtatgc
     1801 gccagcagat tgtaggtgcc gccgtaaata ttatttgcag aaatgatatg atctccctgt
     1861 tttgccaggt tctggatcgt gtagcagata gctgctgcgc cactggctac cgccagagct
     1921 gcggtaccac cttccagtgc tgcaatacgc tgctcgaaga cgccctgtgt tgggttgccc
     1981 agacgaccgt agatctcacc gggatcttcc agtccgaaac gagctgctgc gtgatcgcag
     2041 ttgcggaata cataagaaga tgtctgataa atgggaacgg ctctggcacc ggtcacggga
     2101 tcgggctgct cctgtcccac atgtaactgt aatgtctcga atttcagatt acgctctgca
     2161 tagggctttt tgctcatgtt ctgcctcatt tctgcacttg gatgtgctat gtattattgc
     2221 gtaacgatcc tggcggaacc gttacaattt ctataaaaac atgctaaaac acattgcaaa
     2281 atctgtcaat gtaaaacttt agcgcagcag atatttttgt ccactgcgcc tctattcctc
     2341 tccagattgc attatattac tttacttcac cagccggtcc ttatacttca gccattttcc
     2401 cgtacgtacc ctgttagtga acaaaaatgc ccgtacgacc cagtccatgg tcatagcgat
     2461 ccagacgccg atgacgccaa gtcccagacc ctgtcccagg atatatgcca tgcccacacg
     2521 gcagagccac atggaagcga tcgccacgat catggtaaat ctcacatctc ccgcagcgcg
     2581 aagtgcattg gactgtgcaa aggacaacgg atgcaccagc atacaacata cacagctgta
     2641 acataacaga aaaacagtta ttttcattgt ctccgaactc agatgataga ttccgcagat
     2701 cggccttgca aaaataatca tgggaattcc caacaggatc atacaggcat atgctacttt
     2761 catcattttt ttcagataat ataatgcctg ctctgtctct cctgcaccga tgcactgtcc
     2821 gatcacagtg atcattgcta caccgatggc gttggcaggg atctgctgaa agctggcaat
     2881 actgcctgtt acggcatttg ccgtcgtagc agccattcca aaaccggcga tcagactggt
     2941 ggtcagaagc ttaccgatct ggaaaatact gttttccagt ccgttgggaa caccgatata
     3001 cagaatcttt cccaagattt tcgtatcgat cctccagtga ttgctcacta tgatcttgtc
     3061 actttttcca agcagcacta tgatcatcac agccgacaat attctggaaa acaacgtgga
     3121 caacgcagca ccggcagcac cgatccctgc accatagatc aggatcgcat ttccggcaat
     3181 atttaccaga tttgccacca atgacacttt catggacacc ttggaatctc ctaccgcacg
     3241 gaacagagct gcaccaccgt tataaattcc cagaaaagga aaggacaccg ccacaatata
     3301 aaaataaatt cttgcggcat tcatgacatc cgcctccaca tttccgtaaa gcagtgccag
     3361 caatccccgg ttccagatca gtgctatcgt cataaacaga agggccacac cccctaccgc
     3421 taaaaaaagc tgattggagg cccggcctac catttcctta tctccgtgtc ccatatattg
     3481 cgccgccact accgcaccgc cgctggccat ggccgtgaac aaaccgatca gcaatacgca
     3541 gatactatcc accagagaga ttccggatac cacagcttct ccacaactgg ccgccatcac
     3601 cgtgtccgcc attcccattg cgattgccaa aaactgctct atgatcagcg gccacaataa
     3661 ttttttcagt gcatcctgtg aaaataacat ttttttctct tccgtcatac cttacatcgt
     3721 tcctttatca tcacattaac aactgccaaa atatcacatt gtcattgcat tgtaaataga
     3781 aatgtaattt tatgattcag taactattca gcaccccatt cgcaaaaccg cctctacgag
     3841 gctttcctga ataacatgct tgcatgttat ttgctcatag tggtcacttc gccctataaa
     3901 tagtatcaaa tactcttacg aatgcaagca ttcgacgcgc attcaatact atttacatgg
     3961 ctctgaataa ttacagctcc tgatatacga aatagtccgc agcgaccagg ccgccttcca
     4021 taccctgctc attgatggca aacagtccgt ccttgacacc gacccagcgg cccggagttg
     4081 cttccaccat ctgtcccaca ggctgtaaat tttcttctgc gattcctaca taaaatgtac
     4141 ggtaaatctc cttctccacc ttcatctgga tataaatgac atcactgttc cattcgccca
     4201 gaccggtgcg cacttcatcg tccttcgtcc agttgccggt gcgctgctgt aagctcatct
     4261 ttccgtttat tttctgcacc gcaagtgctg tataacatcc gcccagcgat accagtcctg
     4321 ccacatcgcc gtccttcatc tgttccagat gcagacaggt ggtaatggag aattccggtg
     4381 ccggccattt ctgcaataac aggttactga tatcgcacag ctgcgtcttc gggtctgcgg
     4441 cctgggcatg aagaatgagc tggccgtttt ccagatcata ccattccttt ttataattgg
     4501 cattccactg ccactgtaat cccaaatttt ccccttcgaa gaaatcggga ggtggtggta
     4561 ggggtttagt tgggtttaca gtggctgggg agttggaatt attaaatgga ttggttt
//
LOCUS       DAWDHD010000198         4617 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_1083_length_4617_cov_9.348093, whole genome shotgun sequence.
ACCESSION   DAWDHD010000198 DAWDHD010000000
VERSION     DAWDHD010000198.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4617)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4617)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4617
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1083_length_4617_cov_9.348093"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1218 a          968 c         1462 g          969 t
ORIGIN      
        1 tccttatggt gatcctgttg gccaagccgc tggaagccta ccagctggga gaaacctatg
       61 cgcagaatct aggagtgaat atcaagaccc tgcggatcct tctggtggtg ttgtccagcg
      121 tgctttcggc atgtatcgtg gcatttgccg gaccgatctc ttttgtgggg atcgctgtgc
      181 cgcagctgat ccgtaaactg ttcggcacta caaagccgtt actgatgata ccggcatgtt
      241 tcttaggagg cagtgtattc tgcctgttct gtgatctgct ggcgaggaaa tggatggcac
      301 ccacggaact tagcatcagc acggtgacgg ccatcttcgg agcaccggta gtattgtgga
      361 tcatgatatc acgaaacaga caggagcgtg gataactatg gaaacgcaga agtcttatta
      421 tattacaacg gaaaaaatga gcgtgggcta cgacggaaag cctctgatcg aagaagtgga
      481 gattatgctt cgaaaagggg aaatcctgac gttactgggt ccaaacggtg caggcaaatc
      541 cacgatctta aaaagtattg caaggcagct gtccctgatc gcaggaacgg tgcggttgga
      601 cggagaggat atgaagtcgc tcaccggagc agaattgtcg aaaaaaatgg cagtggtgat
      661 gaccaaacgc ctgcgggccg agctgatgac ctgtgaggat gtggtggcca ccggaaggta
      721 tccttatacc ggacgttttg gtgtgctgcg ggcagaagat catgcagcgg tgcaaaacgc
      781 catggaactg gtgcaggtgg cggagattgg ggattctttg tttgaccgga taagtgacgg
      841 gcagagacag cgggtgatgt tagcaagagc catttgtcag gagccggaga tattaatcct
      901 ggacgagccg acttcttacc tggatgtgcg gcataaactg gaatttttat cgatcctgca
      961 aaagctgtgc agggagagag aacttacggt gatcctgtcc ttgcatgagt tggaactggc
     1021 ggagaagatc tccgatagaa tcctgtgtgt gaacggcagg gcggtggacc ggattggaac
     1081 gccgggggaa gtcatgacag ctgggtatat tacacagtta tatggcatcc ggatgggaag
     1141 ctatgatgag ggcagcagtg atatggagct ggcggcgccg gaaggaacac cgcaggtctt
     1201 cgtgatcgcc ggagccggaa gcggacggaa aatttaccgg cgtctgcaga gagcgggaat
     1261 cccctttgtc accgggatcc tgtatgaaaa tgatctggat tatcccgtgg cgaaggcact
     1321 ggcggcggag gtgatcgcag agcatccttt tgagccggtg gcacaggaaa aaatacaggc
     1381 agcaaagaaa acgatagata agtgcaaacg tgttatctgt tgccgggaga atttcggcag
     1441 tctggagact tttcagagag aacttctgga gtatgcagaa cagctgggga agcagataga
     1501 aaagctagaa agaccggaac aacagagcgg gtgttagaaa acagagaaac gaaattcccg
     1561 ggatgtctat gacatctgtg ggatggagag aacgatggca aaagtaatca tgatacaggg
     1621 caccatgtcc ggtgccggga aaagtctgct ggtggcgggt ctctgtcgga ttttccggca
     1681 ggacggctac cgggtggccc cttttaaatc acagaatatg gcgctcaatt cctatatcac
     1741 caaggaagga ctggaaatgg gcagggcaca ggtcatgcag gcagaggccg cagggatcga
     1801 accgctggtg tgcatgaatc ccattttgtt aaagcccacc agtcataccg gatcccaggt
     1861 cattgtgaac ggggaagtac ggggcaatct gtccgccaga gactattttg cccacaagac
     1921 ggaactgatc ccggagatca aggccgcatt ccgcaagctg gagacctatg cggatattat
     1981 tgtcatcgag ggcgcgggaa gcccggcgga gatcaatctg aaacagaatg acatcgtaaa
     2041 tatgggaatg gcggccatgg tggatgcacc ggtacttctg gtaggagaca ttgacagagg
     2101 tggtgtgttc gcacagcttt tgggaaccct gatgctgctg accgaagagg agcgggagcg
     2161 ggtaaaggga ttgatcatca acaaattccg tggagacagt acaattttag atcccggcat
     2221 tcagatgtta acagaacggg gacaggtgcc ggtgctgggg actgtacctt atatggaact
     2281 tacgctggaa gatgaggata gtctgacgga tcgttttgat gcaaaacatg tggggaagat
     2341 cgatctggcg gtgatccact atcccagaat ttcaaatttt acggattttg atgtgtttga
     2401 gcagatgccg gaggtcagtg tccggtatgt gacaaatgtg agagagctgg gaacgccgga
     2461 tctgatattt ctccccggca gtaaaaatac catgggagat ctgaaatgga tgcggcagaa
     2521 cggactggaa gctgcagtaa aacgggctgc gggaaaagtt cccattttcg ggatctgtgg
     2581 aggctatcag atgttaggct gtgagattgc ggatccgttt ggtgtggagg agggcggaca
     2641 gatccgtggc atggagctgc ttccggtacg gacagtgctg cagaaggaga agcatcgctg
     2701 ccagacagat ggaaagctgg atgctgtgga gggtattttc tcaggactta cgggatgtaa
     2761 gtttgccggg tatgagatcc atatgggaca gacggtgtat tgtgacgggg atggcagcga
     2821 tgcgaagggt accgtggaca aggcagccag accggcaaac agtgcagaaa gcaatcggag
     2881 cgctttctgt gcagacgatg cgacgagaaa cacggaaatc acacaagctg ttatcgctga
     2941 ctccaccggc agaatctacg gctcctatat ccacggtctg ttcgacatgg gagagatcgc
     3001 cgggcgtatg atacagaccc tggcccggga aaaaggcatc tctctggaga acggagtctg
     3061 ggaggattac cgcatcatta aggaaagaca atacgataag ctagcagata ccctgcggga
     3121 atatctgcgt atggaggaaa tctatggaat gctcagagaa gcctatattt cataactaca
     3181 ccggacagga actggcgcag atccggatta cggcaccgga ggaagcggtg agaagtcttg
     3241 tgaaaaaaca ctgggatacc ctggcaaaac cgttggacgg catgggcagc tttgaaacca
     3301 ttacggcgca gatcggagct atactgggaa cggaacagat cgaccttcgg aaaaaaggtg
     3361 tgctgatctt ctgtgcagac aacggaatcg tggaggaagg cgtgagccag acggggcagg
     3421 aggtcacgtt ggcggtggca aagtccatgg cacggaaagg atcctccgtc tgcaagatgg
     3481 cgcagagcat cggcgcggag acgatccctg tggatatcgg aataaatagt gaggaatcca
     3541 ttcccggcgt cctggatcgt aaggtgcgtc ccggcacaag aaattttttg aaagaaccga
     3601 cgatgacgga ggaagagact gtccgggcca ttgtcacagg aatcgaactg gtacaggatt
     3661 gcaaggagaa gggttacgga cttctggcca ctggggaaat gggaatcggc aacacgacta
     3721 ccagcagtgc ggtgacggca gctcttttgc agtgcgaagc agaggaagtc accggcagag
     3781 gtgcgggact tacggaccag ggactgactc gtaagcaaca ggttgtccgc accgcattgg
     3841 aaacctatga tctgtggcat gcggatgcct tcacggtatt gcagacagtc ggagggctgg
     3901 acattgcagg acttaccggt atgtgcatcg gcggcgcgtt gtggcatata cccattgtgt
     3961 tggatggtgt gatcagcatg gcggcagcat tggtggcgga gcggctgttt cccggcgtgc
     4021 gggaatatct gattccttcc catcagggga aagaaccggc ggcagtgaag ctggcagatg
     4081 ctttgcagct gtctccggtg atccacgcgg gaatggcact gggagaagga accggagccg
     4141 ttatgatgtt cacactgctg gatatggcaa tgagcattta tggacagagc gctaccttct
     4201 cagaaatcga agtagaacaa tatagacgtt aacagttcag ccatgaacat atccgggcgc
     4261 aaaaatcatg ctcgcatgat tttttgagcg aatatgttca tgagatgagc gccggtctat
     4321 gagcaagggt agtcacgaag tgacgcaaaa gcgtgatagc gcgaccttgc gattggcgcg
     4381 gctgaaagac ggaacggatg gagaagcaac atgatgatat tgatcctggg gggcagcggc
     4441 agcgggaaat ccgcttatgc ggaagattat ctgctgcgga ctgccggtga taagaaaaaa
     4501 tattatatcg ctactatgca gatctgggat gaggaaatgc aggccaatgt tgacaggcat
     4561 cactgactgc ggcagggaaa aggtttcact accatcgaac agtctactgc actggag
//
LOCUS       DAWDHD010000199         4596 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_1087_length_4596_cov_8.129267, whole genome shotgun sequence.
ACCESSION   DAWDHD010000199 DAWDHD010000000
VERSION     DAWDHD010000199.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4596)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4596)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4596
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1087_length_4596_cov_8.129267"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1215 a         1094 c         1177 g         1110 t
ORIGIN      
        1 acagagaaaa tccctggagg aaaagatcca ggcaaaacag gagttgatca atgcgctgaa
       61 tatccgcatt gaatccgagg aacgggaact ggaagaatta tatgagcaga agaggcgcaa
      121 agaactggag gcagtaagtg acctgatcga ggatgccggc ctggcaccgg aggaagtcac
      181 cgaagtcttg caacaataca ttgagaaccg cagcgcaaat gcatcgtgag attgcaggtg
      241 aaagagagct tgctcacctt aaagaaccgg agctgtaccg cccaaaagtt agaaacagaa
      301 tcaatctaat gactggaggc cgatacacgc ccggtctttt tttgtgcccg tttggttatc
      361 gccaatcatg taatttatta aggcgagtgt ctaaattctg aatcataaaa aactcctttc
      421 aaaatggttt ggtgtaaata ggataaaata ccagaaaaaa atacaaaaaa gtctaatttt
      481 caagtggaaa aagggatgat tgtatcaaaa taaagaaaaa gggtaaatca gatacaaaaa
      541 aagaaaaaat atgagaaatt ttgtactaaa atagccggaa aacagctttt agcgaaaatg
      601 gagataaatg aacattgtgg gacatgcaga taagcaatag agcgggcact tttgaaggca
      661 caagcatatc tgagtgcatt ataacatgac aggggagatt ctgaaaagat gtttgtttgg
      721 gaaaagagat ctgaatacaa aacacccacg gcatggaatt ccaatggaat cccttgtgga
      781 atcccttgcc gcgggcagtg gtttcagact ataaatgttt tattccgcgc ttccttcacc
      841 cttcttcaga ttcttctggg cggtggagag catgagtttg attcggttga gctggttgac
      901 ttcgctggca ccgggatcgt agtcgatggc tacgatgttg gaggaaggat atgccttccg
      961 cagagccttg ataacgccct tacctactac gtggttcggc aggcagccga aaggctgggt
     1021 acatacgatg ttcggtacgc cttccttgat cagttccacc atctcaccgg tcaggaacca
     1081 tccctcaccg gtctggttgc cgatggatac gatcggttcc gcataggaga cgatcttgta
     1141 aatgttggta ggcggatcaa aatgcttact ttcttcaaaa gccttcacgg cagagccgcg
     1201 gaggatatgt tcgatggccc agatacccag cgcggaaatc tttgcagtct tcttgctggt
     1261 gcctaagtgt tctgctttgt agatctggtt gtagaaacag tacagcatga agtcgatcag
     1321 gtcgggtact acagcctccg caccttcgct ctccagcagc tctaccagat gattgttgcc
     1381 tgcaggagag aatttcacca gaatctcacc gaccacgccg acacggggct tcttgatatc
     1441 cagtaccgga atcgcatcga aatcacggat gatctgcaca cacatcttgc ggaacttcgg
     1501 aatgctgata tgcttcgcgg agacaaattt ctgtatcttc gcaagccatt ttttgtgcag
     1561 ggcatccacg gaacccggag ttgcttcata aggacgcatc cgatatacac aacgcatgaa
     1621 gatgtcacca aattccgcac ccacagcggc acgtaacagc agatccgcat tcagatggaa
     1681 gccgggattg ctctcgatac ctgccaggtt cagggagata acgggaatct gctccatgtc
     1741 tgctttcttc aaggcacgac ggatgaagcc gatatagttc gtcgcacggc atccgccacc
     1801 ggtctggctc atgatgatgg ctgtcttgtt caggtcatat ttaccggaca gcagagcttc
     1861 catgatctga cctactacca gcagggaagg atagcaggca tcattgttaa catatttcag
     1921 accaacatcc acagagtgct tgttatcgtt gtccaatacc acgaaattgt aaccgcaggc
     1981 attgaacgca ggctgcaaga tctcaaagtg gatcggggac atctgcggac agatgatggt
     2041 atagtcctta cgcatctctt cggtaaacgg taccttctcg atagcggaag aacggatgtt
     2101 gcggtgcttg cccatctttt cacggacacg gattgcagac aacagggaac ggatacggat
     2161 gcgggccgca cccaggttgt tgacctcatc gatcttcagg caggtgtaga ttttgtcgga
     2221 gccggagagg atatcgttca cctgatcggt ggtaacggca tccagaccgc agccgaagga
     2281 attcaactgg atcagatcca gattctccac cgtctttaca tagcttgcgg cagcgtaaag
     2341 tctggaatgg tacatccact ggtcggatac aatcaaagga cgttccggtt ttgccagatg
     2401 ggagatggaa tcctctgtca gtactgccat atcgtaggag gtgatcagct cgggaatacc
     2461 gtggttgatc tccgggtcta catgataggg acggcctgct agcacgatac cgcgcttgcc
     2521 ggtcttgcgt aagtattcca gagtctcttc gccctttttc tccatatcgt gacgggcatt
     2581 tgccagttcc ttccaaccct tttcgcaggc tgcggcgatc tctgcggctg gaatctcggt
     2641 gaattccttc gtcagcgcat ccgtcacggt accaagatct ctaaaggaca tgaacggatt
     2701 gcggaaagtg atctcaccgc cggcgatttc ctctacgtta ttcttgatat tctcagaata
     2761 agaagtaacg atagggcagt tgtaatggtt gttggcatcc tcgaactcgt tgcgctcata
     2821 gaatagcgca gggtagaaga tgaacttgat tccctgacga atcagccagg tcacatggcc
     2881 gtgagccagt ttcgccggat aacactcgga ttcactgggg atggattcaa tgccaagctc
     2941 gtagatctta cggttggagc tgggagagag cacaacgcgg tagcccagtt cggtgaagaa
     3001 ggtgaaccag aacggatagt cctcgtacat attcagcact ctggggatac caactacacc
     3061 tctgggagcc ttgtcagcgt ccagagattc ataagagaac agtctatgta atttgtattc
     3121 gtataaattc ggtacattgt gggcattctt ctgaccgccg ataccacgtt cacagcggtt
     3181 gccggagatg aactgacggc caccggagaa tttgttgatg gtcagacgac agttgttggt
     3241 acaaccctta catttcgcca tgctagtctc gaactggaga tcgcagatct tctgataatc
     3301 cagcattgtg gtcacataac cgtcttcgta atgttctctg gcgatcagag ccgcaccgaa
     3361 agcacccatg ataccggcga tgtcgggacg gatcgcctgg cagtctgcaa ttttctcaaa
     3421 gctgcgcaga acagcatcat tgtagaaagt accgccctgt accacgatat ttttaccaag
     3481 ctctgaagca tcggagactt tgattacctt aaataaagca ttcttgataa cggagtaagc
     3541 aagtcctgcg gagatatctg ctacggaagc gccttccttc tgcgcctgtt ttactttgga
     3601 gttcatgaat acggtacaac gggtacccag atcgatagga tgttctgcaa acagggcagc
     3661 ctgagcgaaa tcctgcacgg tatagttcag ggatttcgca aaggtctcaa taaaggagcc
     3721 acagccggag gaacatgctt cgttcagctg tacgctgtct acggtctggt ttttaatctt
     3781 gatgcacttc atatcctgac cgccgatgtc taagatacag tctacgctgg ggtcaaagaa
     3841 agcagcggca tagtaatggg ccacggtctc tacctcgccg tcatccagca gaaaagcagc
     3901 tttcatcaac gcttcgccgt aaccagtgga acaggagcgt acgatctcca cgccttcggg
     3961 caggagagag tagatctcct tcagggagcg gatcgctgtc ttcaaggggc ttccgtcgtt
     4021 actggaatag aaagagtaca gcagggatcc atcttcacct accagagcga tcttcgtggt
     4081 ggtggaaccg gcatcgatac ccaggaaagc cttgccctga taggactgta agtctccggt
     4141 ggtcacgtga tgcttgctgt gacgctccac gaattcctga taagcagcct tgtccgcaaa
     4201 cagaggctcc atacgttcta cttcgaactc catcttgatg tcggaggaca gctttttcac
     4261 catgtcagac agactgatgc agacttcctc cttcgcattt agtgcggaac cgatggctgc
     4321 gaacagatgg gagttgtcca cgtcaatgat gtgctcgtca tccagattta aggtacggat
     4381 aaaggcagct ttcagttcag acaggaaatg taaaggcccg cccagaaaag ctacgtgacc
     4441 gcggatcggc ttaccacagg ccagaccgga aattgtctgg ttcaccactg cctggaaaat
     4501 agaagcagac aggtcttcct tggtagcacc ttcgttgatc agcggctgga tttcagtctt
     4561 ggcaaataca ccactgcggg ctgcaatggt gaagag
//
LOCUS       DAWDHD010000200         4423 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_1140_length_4423_cov_15.424908, whole genome shotgun sequence.
ACCESSION   DAWDHD010000200 DAWDHD010000000
VERSION     DAWDHD010000200.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4423)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4423)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4423
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1140_length_4423_cov_15.424908"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1263 a          909 c         1239 g         1012 t
ORIGIN      
        1 ctttaaactc atatataata tcctttcaga gtaaaagtga tttttgaagc atttatggag
       61 gtttttacca tgtttaatat gaataataaa aaagtaaaac agcgaatctc cgcagctatc
      121 atcgtgctgc ttgtgattgc tatgatcgta cctactataa catctatttt ctactaattc
      181 aactgcggat ggaacagtag atgtgcccga aggaagggca gaggagtatt atgaataagt
      241 atcgtaaggg gttttacggc attctgttat tgactttgac aatgctgttc ggaatgacag
      301 cgcaggccaa gacagatgat actattaaga caggaattta tgccggggat gtggaactat
      361 ccggaatgac ggcacaggaa gcaacagcgg ttatcgaaga acatatcgaa agtctgaagg
      421 atgtggagat cacactgctt gcggcaaatg atcacgatgt gaccaccaca gccggagatc
      481 tgggtgtgac ctggaaaaat cctgagctgg tgcaggaagc attggaactg gggacacatg
      541 gaaatgtcat tgagcgttac aagatcctga tggatctgca gcatgaaaat tacgtatatc
      601 ccattgaact ggattttgac ttacaggcga tcaacgatct gctgaccaga tgcactaagt
      661 atgatcagga agccatcaac gtctctctga agcgggacgg cggtaaattt accgtagtgg
      721 aaggtcagac cggatatgtg ttggatgtgg agaaatctat cgatgcggta tatgactatc
      781 tgacagaaga atggaatcat gaggcctgca gcattccttt ggagatcgta gtggatgagc
      841 cgaagggaag tgctgaggaa ctggcacagg taacggatgt attgggaagc tttaccacat
      901 cttataagac atccggatcc tccagaagtg caaatgtggc gaacggctgc agtctgatca
      961 acggaacgac tctgtatccg ggagaagaat tttctactta taagacggta tctccttttt
     1021 ccgtggcgaa cggctattac atggcgggtt cttatgtcag cggaaaagtg gtagacagtc
     1081 tgggcggcgg tatctgccag gtatccacta ccctgtacaa tgcagtatta cttgcggagc
     1141 tggaagtaac ggaacgttat aatcattcca tgatcgtagg ctatgtggat ccttccgcag
     1201 atgcagcgat cgcggagagc tccggcaaag attttaagtt tgtaaataat acagatgcac
     1261 cgatctacat agaaggctat acgcatgaca agcagatcac gtttaacatc tatggcaagg
     1321 agaccagagc tgccggacat tcggtacgtt atgaaagtga agtgctggag acgatcacac
     1381 cccctgcgga tcagatttat gcggatgccg gtcaacccat cggttatatc gtaacagaga
     1441 gtgcgcatat cggctataag gcaagactgt ggaagatcac gatggaaaac ggtgtagagg
     1501 tcagcagaga acaggtcaac agcagtactt ataagatggt gccccgcagt gccactgtgg
     1561 gaaccgctac ctcggatccc caggcgtatg aagagatcat ggcagctatc agtacagcga
     1621 atattgacca tgtgaaaaat gtggcagcag cgttaaatgc aagagcagca gcggctgctg
     1681 ggcagacaga aatcgtagac gactaaaggt gagacaatga acagcggtaa ggtacccgtg
     1741 aacgtcttga aacgttccgt tctcagacaa ctgaagaata aaagatcgga aatagcaaat
     1801 agcgcaggtc tgggggcaga ctgcgctatt tttgagcctt tttcggaggg gcagctggtg
     1861 acctgtgttc aggagggcgt agtggagctt tgctgtgaga acgatctggc ggaagcggaa
     1921 agagaagatc catccgtgat gcctatgcgt cgctttttcc agaaatgtgc caacaatctg
     1981 gcaactgccg gtgcagagcc cgtggcagcg gaattggtga tcttcctgcc ggagagcgtg
     2041 gaagaaccgg aattgaaagc actgatgtcc gaagcggaag gtattgccgc agagttaaac
     2101 atacagatta tcggcggaca gaccagagta agttccgcgg tacggcagcc cctggccacg
     2161 gtgaccggat atggcatccg caagactggt gcggtgcaga tggatgtgcg gaagaagctg
     2221 gcgggacagg atattatcat tacgaaatgg atcggcctgg aaggcactgc ggatctggcg
     2281 gcccggaacc aggaagaact cctgacgaga tacccggcgt atctggtgga agaagctgcg
     2341 gcgtttgatc ggtattattc tattttgccg gaggccgcta ccgccgtgaa gtccggcggt
     2401 tgtaccatgc atgatatttc cgaaggcggt gtatttgcag cgctgtggga gatggcagag
     2461 ggtgcaggtg ttggactgac aatagatatg aagaaacttc ctctcagaca ggagacagtg
     2521 gaggtatgtg aattctgtaa tgtaaatccg tatgaactca gatccggcgg cagcctgatc
     2581 attgcaagcc cggagggaac cgcagtggtg gaagcactgg cggcagaggg aattccggca
     2641 gtgatcgtag gaagatttac agacagtaat gaacgactga tcctgaatga ggacgaagtt
     2701 cgttatatgg atcgtcctca gagagatgag atctataaag aaacagaata aaaggagata
     2761 gaggcatgag agaagaatta ttagcgatca tagagaagaa cagcaggatc gatctgaagg
     2821 aactggcagt gatcctggga gtggaagaga tcgatgtggt aaatgaactg gcagcactgg
     2881 aagcagaagg gatcatctgc ggttatcata ccctgattaa ctgggagaag acagacattg
     2941 aaaaagtaag cgctctgatc gaggtacgtg taactcccca gagaggccag ggctttgaca
     3001 gtattgcaga gcgtatctac aaatatcctg aagtacgcag tgtgtatctg atgtccggag
     3061 cgtatgatct gatggtcatt ctggagggta agaccctgcg ggaggtatct aattttgtat
     3121 ctgacaagct gtccacactg gattccgtgc tgagtacagc aacgcatttt atcctgaaga
     3181 aatacaagga tcacggaacc gcatttgctg taaaaaaaga agatgaaaga gagatgatca
     3241 ccccatgaga aatccattag ctgataaagt agtggatatc aaaccatcgg gtatccgcaa
     3301 attttttgat attgtaagtg agatgcagga tgcaatctct ctcggtgtgg gagaaccgga
     3361 cttcgacact ccctggcaca tccgggatga gggcatctat tccctggaaa agggaagaac
     3421 cttttacacc tccaatgcag gtctgaagga tctgcgtcag gagatctgca acacgatcca
     3481 gagaaaacag ggtgtaacct atgactggca gcacgaagtg ctggtaacgg tcggtggttc
     3541 cgaggctatc gacatcggac tgcgtgccat gtgtaatccg ggagacgaag tgctgatccc
     3601 ccagcccagt tatgtatcct atgagccctg tgcggtcctg gcaggagcaa ctccggtgat
     3661 cattgatctg aaggcggaga atgaattccg cctgactgca gaggaactgg aagcagcgat
     3721 cacggacaag acaaaagtgt tgatccttcc tttcccgaac aaccctaccg gtgccatcat
     3781 ggagaagtct gatctggaag ccattgcgaa agttatcgaa gaacatgaca tttttgttat
     3841 gtctgacgag atctattctg agcttactta taaagagaag catgtgtcca tcgtcagcct
     3901 gcccggcatg aaggagcgta ccatttacat taacggtttc tccaaggctt atgccatgac
     3961 cggttggaga ctgggctatt gctgcggacc ggctgctatc atccagcaga tgacgaagat
     4021 ccatcagttt gccattatgt gtgcacctac caccagccag tatgcggcag tggaagcact
     4081 gaaaaacgga gatcccgaca tcgaggaaat gcgcacggcc tataatcaga gaagacgctt
     4141 tctgatgaat gcgtttcgtg aaatgggact ggaatgcttt gagccttacg gcgcattcta
     4201 tgtattcccc tgcatcaagg aattcggcat gaccagtgaa gaatttgcca cccgtttcct
     4261 ggaggaggaa aaagtagctg ctgtacccgg gaccgcattc ggtgacagtg gcgaggggta
     4321 tcttagaatc tcctatgcgt actccctgga gaagctgaag attgccatcg gcagactggg
     4381 acatttcgta gaaaaattaa gagcagaaca aaataataaa taa
//
LOCUS       DAWDHD010000201         4372 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_1162_length_4372_cov_10.857077, whole genome shotgun sequence.
ACCESSION   DAWDHD010000201 DAWDHD010000000
VERSION     DAWDHD010000201.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4372)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4372)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4372
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1162_length_4372_cov_10.857077"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1022 a         1223 c          873 g         1254 t
ORIGIN      
        1 ttccggcgaa aagggcacaa aaaaagcctc gcataatcat cactcccaaa agagtccctt
       61 attactatga ttattatgag gctgattctt tgtcctattc tattatatgt ttaatgtgag
      121 ctgtacttcc tgctccgcct gctgtttgaa ttcctccacc tgcaccgggt tcagttccgg
      181 cagttcctcc aggctcttca caccaaagca gcgaaggaac tgttccgttg tgccgaacag
      241 cagaggtctt ccgggagcat ccagccgccc taactccgtg atcagatcgt attccaacag
      301 cttattgatg gcatgatcgc agctgacccc tctgactctc tcgatctcca gtcttgttac
      361 cggctgctta taggcaatga tggacagggt ctccagaacc gtatccgtca atgtcatctt
      421 tcggggagtc ttggcgatct tgatcaggta ttcatacata tccgacttgg tacacagctg
      481 taccgaattg tcaaactgtg tcagtccgat gccacggttc tcttcctcat accgcgccgc
      541 catatctttt aaaatggatt ttgtggtctt cacatcctct tcgatcactt ccgcaagtct
      601 gctgatctcc acggaatccc ccatggtaaa aaggatggct tctattactg cttctgcttt
      661 actgcgttcc atgttccgac ctcactgtat cttaaacgtt agatgtaatc ataatatcat
      721 cgaatatatt ttcctgactg atgttgatct tgccggtctt catcagctcc agaatgatca
      781 agaaagtgac gatgacctcc attttgctgt tctgcttctc cagaagcttt cggaaactga
      841 aggtcttatg ttctctggca taagcttcca catacagcat cttgatgtcc atatccacct
      901 cgtccttttc gatattgcca tactggctgc ggacagggtc gatcttgtcc tcctgtttct
      961 tcacaataga tttgaatatt tcatgaagct tgttcaagtt catatcgccg atcagctctt
     1021 cgtaatcgat gggctgtctg taggcttcca cttccttcgg aagtctctgt tctctgtaca
     1081 tatttctggc agcatccacc tgccggtctc tgagttcgaa ggacatatat ttgtacatct
     1141 tgtattccaa cagcttctgt accagctccg ctctgggatc ctcttcctcg ccgtcctcgt
     1201 tcacttcctt gggcagcaac attttgcact tgatatcgat cagtgttgcc gccattacca
     1261 ggaattcact catgacgttc atatcttccc gctgcatctg cctgatgtaa tccagatact
     1321 gggccgtgat ctccacaata ggaatatcat agatgtctat tttgtttttc tcgatcaaat
     1381 gaagcaacag atccagagga ccttcaaatg cttccagttt tacagatatt gccatgtaat
     1441 aaaactccta tttcccctca tcactcccac cggcctcttc tccccccggc agcagatcca
     1501 cttccaggac ctctccagga ttgaacagcc ggatcggaat cgtatgcatt cccagtcctc
     1561 tgctgagcag cattctggtt ttccccagtg tgaactctcc accatcatat tttggaaaaa
     1621 gtctcacatt gggagacacc acgccttttc cccagatggg gactctcacc ataccgccgt
     1681 ggacatggcc ggccagcacc agatctgctc cccagtccgc gtatttcgga aaataatccg
     1741 gattgtgggc gatcaggatc gtatactttt ctgcggaagg ttgtcccaac agacttttca
     1801 gatactccgg atccatgggc tggatcccga agcgcttgta atacagcttg tcgatctccg
     1861 atccgtaaat gcagatcccg gattcttcca gaatggtatg ttcattcacc agcctccgga
     1921 ttccgatctt ctgcagggcc tcctcgtacc gctccgccat atctccgtag ttctccgggt
     1981 acagtttcaa acggtgttca tgattgccgt tgccgtagta gatcggatat ttcccggcaa
     2041 gcttcgtaag caggtccacc gccgtctcca gacttgcctt cggctttgcc gtcagaatat
     2101 ctccggcgat caggatcatg tccggcctta tctcatcaat ggcctgcagc aggcgctcat
     2161 tttcttttcc gtatctctta ttgtgcagat ccgccagaac cactgcctta acaggctttt
     2221 tgattctctg atcccgcagg aaataatgcc gtaccacaaa acgattgctg tcgtacagca
     2281 ttatccatac aagaaccacc aggatcactg cgatcacggt aatcactaca tccaaattct
     2341 ttgcccccat ggtctatttc cagttaattg ccaatatgtt ctatagtata acatattttt
     2401 cgggcttaca aaagataaaa ttccattatt ttaaagaggt agtccttgta taccgctttc
     2461 gccacatcat tttcatacag gatcggaacg cttccgatct ccactccgtt caacagatag
     2521 accgctctgc ctgcctctgc cccctttgcg acaggcgcct gtgcctcctc cggtaattct
     2581 atggttttct tcactccgtc cagcgaattg ccttccgtat ccagataccg gaattcgctc
     2641 tggtatctga caggtaccgt atcttccacg ccaccttcta tccgaaggtc ctccaataca
     2701 tccgtattct catccagata caggtcactg acattgaaac cgtaacttaa cagtgtctga
     2761 gcatccttaa aacgtgcctt gtagtccggg gctcccatga ccacggcaat caggtcaata
     2821 ccatccttcg tggcagtggc agacaggcag aatttagcct tggaggtgga tccggtcttc
     2881 agtcccgtag cccactggta ctgtttcaac agcttgttgg tactgttcag ggtaaatgta
     2941 gagctgccct gtctggtctc atggatgatg tcttccatcc agataccagt atactcaaag
     3001 acctgaggat atttcaccgt aagctctctg gacataatgg ccacatccat ggctgtcgta
     3061 tagtgtccgt cggaatccgt cagtccgcaa caatcctcga aatgtgtatc caccattccc
     3121 agttctgccg ccttttgatt catctgatcc acgaaagcct cttcactccc cgctatgtat
     3181 tctgccaccg ccacactggc atcgttgccg gaagctacag caatacattt gatcatggtc
     3241 tccaaggtct gtacctcgtt ctccgccagg aatacctggg agcctcccat ggaactggcg
     3301 taagcactga cggtcaccgg atcctccagt tttatcttcc cctgatccaa tgcctcgaat
     3361 gtcaatagca atgtcatgat cttggtaata ctggcgggac ttctgcgctc tctggcattc
     3421 tgttcaaaga tcactttccc ggtagagctc tccagaagca cggcactggg agcttcgatt
     3481 gccgccggct ccgcagatac actgatcccg tcccaccaga atacctgtat gctgaccgta
     3541 aaaagtaaga tccctgtgaa aatccttttc ctgttttttt gcataaaaaa gccacactcc
     3601 cttatccttt cttctaaaag atatggtcgt atggccttgt atatacctgt aatccaaccg
     3661 tccggaacac tacagtctgg tgatcagtgc ctgacagcgt tccttcaggg cttcaccctt
     3721 gctacgcaca tcgatgtgaa atactttgat cagctggacc acataatcca cagctacaat
     3781 cgccagaatt acctgaatca aacggatgcc cacctggatc tgtactctgt caataatgtg
     3841 atacatggca tcgtgaacga actgtacgat tcctattgca taaaagcccc aggcaacagt
     3901 actttccagg cagatgatac cacggtaatt gaatggcttg ttgttataat cccaccagag
     3961 ttctccgagg aaacggatca taccgattcc caccagatat tcaaaagtgg tggccagaat
     4021 ggctccaaga aaatacagct ttaccagttt cccgcgcagg ggacttaaaa tcagatatcc
     4081 cagcactccg ccgaaaccgt agatcgggca aaagggacct ttgccgaagc cacggttggt
     4141 cagcttgtga ttgcagaaag acatataggt agattccaat gcccagccaa gaatactgta
     4201 caatacaaac gctgctaaca gatgatagat atctgttccc agtatttcat aattccacat
     4261 tttgtttccc tcataagtac aaagtccccg gtgattacgc cgaggactgt tgtcttgcat
     4321 tagttatatt tacgttttct tgctgcttca gacttcttct tacgttttac gc
//
LOCUS       DAWDHD010000202         4354 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_1168_length_4354_cov_9.417539, whole genome shotgun sequence.
ACCESSION   DAWDHD010000202 DAWDHD010000000
VERSION     DAWDHD010000202.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4354)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4354)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4354
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1168_length_4354_cov_9.417539"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1072 a         1189 c          793 g         1300 t
ORIGIN      
        1 aagacaccgg tggctccgtt ttcaaattcc atataagccg tcacatcatc ctcgccctcg
       61 atatcatgcc attttccttc gtggcagaag gctctcacct tcacaggcat gccgcacagc
      121 cattgcagca gatccagctg gtgcggacac tggttcaaca gcacgccgcc accttctccg
      181 tcccaggttg ccttccagcc cgccgcatca taatagctct gattacgata ccagtcggtg
      241 atgatccagt tgacacgtcg cagctgccca agttcgccgc tctggatcat ttcgtgaagt
      301 tttctgtata cacagttggt tctctgattt aacatgatgg caaaggatct gtcagacctt
      361 tccgctgcct cattcatctc ccgtacctgt ttcgtgtaga ctcctgccgg tttttcgctg
      421 atcgcatgca gtccatgctc caatgcatag atgacgaatt ccggatgcag ataatgtggt
      481 gttgccacca gaacggcatc acagcttccc gaatcaatca ttttcctgcc atcatcaaat
      541 accttgaccg tctccggaag attgttcctt gcccagtcca ggcggctctg ccggatatcc
      601 gctacggcgg tcagttccat atccggcacc atccctttca acacattgcc tgcatgaccg
      661 gttcccatac tcccaatacc aataatgccc agtcttacct tttccattta gacacctttc
      721 ctttcattct ctgatgtatt tctttgatgt tcttattgta acttctctac gattgtaata
      781 atatgtatat aaaatactaa ttttatacaa aataacgacc tttggagtgc ctatgaaaac
      841 caaaatgact attccctatt ttacaattga tgcaccctat gaactggctt accgcaacaa
      901 cggaacctct tctcctgcca gaccgcatac gcacaacgct ctggaaatat atcttacaat
      961 gtccgaccta ccggatgtcc tcttagacga cactgtctcc agtgtttcga aaggatctct
     1021 gatcatcatt ccgcctcacc atgtgcatca gctgttcaat cagaagctga ccatctatga
     1081 gcgctacgtc ctgaatatta acagcgactg gctgtatact gtccttggag ccggttccgg
     1141 actcatgcct tatgcagatc cggctttctc tcctgcgatc ctccggcttt ctcccaccgg
     1201 gcttaccggg ctctgcagtc agttggatca ctatttgcag ctgcatcctc agccctccct
     1261 ttctgcctat gcggattttt tctctctgct aagtatactg gatagccgga tccagcacgg
     1321 cctacaggca tcttctaccg gacagcgttc tatttccaag tcccagaaaa acatcaatga
     1381 ggtcatggcc tttatcaacc ggcatctgac agagccactg accctggaaa gcattgctgc
     1441 agaattctac ctgaacaagg actatctcgg gcgcctgttc aaggaacata cccatgccac
     1501 catcgggcac tatatctccg tgcagcgggc taacctcgcc cagacgatgc tggcagaggg
     1561 ccggaccgtc accgaggtac aggaacttct cggcttctcc agctatgcct atttctttaa
     1621 atttttcaaa aaaatgaccg ggatctctcc cagtcaatat cgtaaagata acactatgcc
     1681 tctgtcgcat tctccgtctc cacattccaa aaaaggaact gtagcctgtg tataaacaca
     1741 cattgcagtt ccttttgctt ttacctgtct acttcacaag ctatcttatt atttagtcag
     1801 cagcatcata atgtcattgc ttcttgccac gaacttggtc atggcatcgg cagacagagg
     1861 tgcgtgctga agctttgcca gatcgtacag ctgctcgcag atcatttctg cattctcacc
     1921 atcctggtgc tcagtaatgt actgtaccag aggatggctg gcattcagga tcagggtctc
     1981 tccctcaccg ccgaaggcac tcatgtccat accgggcatg gaatacagct tgctgatgct
     2041 ctccgcctgc tcttccatct ctttctcggc cttcttggaa gtcttggact tcagggcatc
     2101 ggtcacatcc gcatcaatac gctggaactt gattccctcg ttctttttct tccgcagaaa
     2161 gagtgctcac acaagttccg ttggcaatgg cggtctgtct ggactgttcc tctctgtctt
     2221 tccagagtac tctttcatat tccgcagact ctctggcaca ttcccgcagg atttctccgt
     2281 ccaataccct ccacatatgc tccgaatctc tgtacagata cggcaattgc agtacattca
     2341 tctccggtgc ctgttctgcg atctgcgaca gggaaactct tgcaaaagca atgcctccgt
     2401 atttgatctg ttccagtact tcgctttccg aacccagttt tgcttcactc tccacgatga
     2461 tgtggatccg tcctccggta cgctcctgca ccagttctgc aaaacctcag cgttacccag
     2521 cgccgcatcc gggtgccatt gattcgctcc gacgtctctg tgaccgatgc cgctctcgtc
     2581 caaagttccc ggcagatgca tctgcgctgc cgttccttcc gtcaatatta ccttccacgt
     2641 tccctccagt gaaatcccca ccgcaatccc tctgttctta tataattttt tatatttttc
     2701 agtcagtcat cacgaaatat agttagtttc attatagact tcacctcaaa ttcaatgcaa
     2761 cggattctcc ttcatgttct atgcaaaaaa gaaccacatt accgatattt ctatcagtaa
     2821 tgcggttctt gaattcttat cagtatgccc acggttcaaa tacaaaatgc cgcataaagc
     2881 ggaaccacct ttttgttgtc ctcaaacgca aagtttttcg cggaaagttt gatcgcgtaa
     2941 gccggcttat aggtatccat atagaccttt aagcttttgg ctctggtatt atcggcggat
     3001 ttcacctcaa tcgggatcag ctgaccatcc cgctggataa cgaaatctat ttcagcccca
     3061 cgctcggatt cccagtagta agtgttataa ccattgatgg caagctgtac attgacataa
     3121 ttttctgtca tgccaccttt aaaatcattc agttcctcca ccatatagag aatatcgttt
     3181 gcagccaaat ccttcttggc gcaaagcagc cccagatccg aaacataaat cttaaacgca
     3241 tcaatatccc gataattttc caaaggcttc ttgatctgct ctaccctata gacctgtgac
     3301 acaattcccg acagacagag ccattcaatc gcattttcaa attcagaagc acgtccaccc
     3361 tttttgatca gcttatattg aaagcgggta tttttcttgg aaagctgaac cgtaatatta
     3421 tcataggcaa gtctggtttt cttaatttca ttcagattat tgtatttgcc catatcattc
     3481 agataacttg tcagaatggt atcctgcgta tggcggatca ggatataatc ccctgtctgg
     3541 acaaactgca tcacacactc tggcataccg cctaccacaa gatactgacg gtaaagctgc
     3601 atggctgcat catggagtgc ggatggcatc ggccgattcg tttcaaaaca ttttttaatc
     3661 tgctctacaa gttcatcctc tcccacagca atcataaatt cttccatatc catcggatac
     3721 agcgttttca tatcaacctt accaacggga aatgaaaatt ttgctctgtt gacggcaacg
     3781 cccaacaggc tgcctgccac aataatatga taatccgggg catcctcaca aaaatatttc
     3841 aatgacgtta aagccctttc gcaaagctgt acctcgtcaa atacaatcaa tgttttttct
     3901 tttacaatcg tctgacctgc aatatgagat aaaatcggta tcaaataatc aggactgatc
     3961 ttttcctcaa aggtttcatt cagttttgga ttcgtttcaa aattgaaata ggctacattt
     4021 tcataatgaa ttcgcccgaa ctctaaaata gaataagtct tccccacctg tcttgccccc
     4081 tgcaaaataa gaggtttgcg gtgttcattt tctttccatg cttccagaaa acccatgatt
     4141 tttctataca tgatccatcc tccttaaagg tcctgaatat agtataatta ttattcatgt
     4201 aaaattcaat atgtttttgt gctgtttttg cattaaaatg catgaatatt tttatgaaat
     4261 catcattttc cgacatgttt gctatggttt ttacattttt tggtaaatag caaatttaac
     4321 ttccgttttg caaaggtaac ttcttcttga atta
//
LOCUS       DAWDHD010000203         4291 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_1192_length_4291_cov_9.330737, whole genome shotgun sequence.
ACCESSION   DAWDHD010000203 DAWDHD010000000
VERSION     DAWDHD010000203.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4291)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4291)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4291
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1192_length_4291_cov_9.330737"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          929 a         1102 c          976 g         1284 t
ORIGIN      
        1 ctccgtctcg tgggctcgga gatgtgtata agagccatcc agtaccgctg ccgccagcac
       61 tgctgtgatc gcattttccg cttttttatt tttcatgcca gtttcccctt cctgcaagaa
      121 tcgttataat ttgttaatga ttatacctta tttttccgca ttcgtcttta cgaaagagtt
      181 attttttgat atttttaagg tatgacttta aataagaaac tattggggga ataaaatctt
      241 atgatacatc tttttgcact gctttcactg cttttgtgcg gtgtctgcta tacaggcttt
      301 cagaacagtt ctcatttcca caatacaagc aaccggcgtt ctcttctgtt gcttgtattt
      361 ggaggtgccc tgcttctgcg cctgctcctt gcatacacca cccacggctt cagtaatgat
      421 atcgcctgtt ttgcagcctg ggctgaccgg attttcactt tagggccagg gcagttctat
      481 tctgccgaga tgtttacgga ctatccgccc ggtttcatgt atgtattgta tctgatcgga
      541 gcgttacgat ctctgctgca gattccttat tattccgatc tgcatatttt actgttaaaa
      601 ttacccgcaa ttctctgtga tattgcctgt gggtttctac tctacagaga ggcggtaaag
      661 cgactgcatt tttccgatct gcagggaatc tttgctgcct ctgcgtatct gttccagccg
      721 gcggtcattc tgaattcttc ctgctgggga caggtggatt ccgtatacac gctgatgatc
      781 atccttatgt gcctgttcct catgaaggga aacctgcttc ctgcctatgc tgtctacgga
      841 ctgggtgtcc tgttaaagcc acagatgctg atcttcacgc cggtactgct ggcagggatc
      901 tgggaccatg tgttcttaca ggatttttcc tggcggaaat tcttctataa tttgtgcggt
      961 ggtctggttg tcatctgcgg gatgctcctt ctgtgcgctc ctttcggatt aaccgctgca
     1021 atctcacaat atacttcaac gttgggatcc tatgaatatg cagccatcaa tgcctacaat
     1081 ttctggggac tattaggaat gaactgggtc gaccagaaca cgattttcct cttcctgccc
     1141 tgtaagacct ggggcactat cgtgatcctg ctgatcgtac tctttacctt ccttattgca
     1201 gcccgctgcc ggaaagagcc ttcaagatat ttctgtctcg gtgcctttat catcctgacg
     1261 atgttcctgt tctccgtccg tatgcatgag cgttatatgt atcccgctct ggccctgttg
     1321 cttttctgct gcctgtaccg gccctctaca cctctctgga aatgcttcag tggatttgca
     1381 gtgctgcatt tttacaacac cgccaatgtg ctgtaccatt acgatccgca gaattatgac
     1441 cgcaaggctc ccatcattct gctggtatct gccggaatgt tgtgctgcct gtattatttc
     1501 tataagatca tctggaaata ttatgttcac gatgaaacag ggactgccac caatgcgaag
     1561 acgcagaccg caacaggccg cagagcttcc gggcatactg cttccacaag atctgccgca
     1621 ggactcggac aaagacttcg agaatatttt ctctctcccc tggagcccat tccttccgaa
     1681 gagagaatcc atttcacgaa accggatctg tgcctgttgc tggcaatcgg tattctgtac
     1741 agttgctttg ccctctatga tctgggagac cgcaaagctc cgacaacaac ttatgatatg
     1801 tcgggagaat tacaggccat cgagctggaa ttccccgaag atgcgctgcc tgtgactatg
     1861 gccagttatc tggctccctg gcaccagcga cattttggga tggatgtcaa aagtaatgcc
     1921 gaagactcct ggacttatct cggtgagatc attttaaaca atgtcttcac ctggcagaat
     1981 gtctccctgc aggatctgct gacacaggcc acagaaaaca gtacttctga tgtgtctgcc
     2041 acaaccagat atctgcgtct gtcattgacg gataatgatg cctctctgat tgaactggtc
     2101 ttcctggacg cgaatggcaa catcacacgc ccgttgaacg cagatgccta ccctacactg
     2161 tttgacgaaa gcggtctgta cccggaacgc tattccttcc gcaacagcat gtatttcgat
     2221 gaaatctatc acgcacgcac ggcttacgag ttccttcacg gcctgccgac ctacgaaaat
     2281 acccacccgc ctctgggcaa gatcttcatc gccctgggtg ttgccatctt tggaatgaat
     2341 cctttcggct ggcgtatcat gggtactctg ttcggcattg ccatgctgcc gtttatctac
     2401 ctgctgggga aaaaaatgac aaggaataca cctgcggcag ctctggcctg cttcctgttt
     2461 gcctttgatt ttatgcattt tacgcagaca aggattgcca ccatcgatgt ttatatcaca
     2521 ttctttgtga ttgcaatgta ctattttatg tactactatt gttctatgag tttttacgat
     2581 acaccgttgt ataagacctt tgtgcctctg ggcttatgtg gcatctgcat gggactggga
     2641 attgccagca aatggaccgg tatctatgcg ggctgtggac tggcactgct gttttttgcg
     2701 catctgctgc gacgctaccg ggaatacctc tatgccaaag cccatcccgg aaaaagcacc
     2761 aacggcatgg agcatcagca gattgtaaag agattcccgg attatacggt aaagacgatc
     2821 gacttctgcc tgaccttttt cgtgctggtt ccggcagtga tctatctgtt gtcctacctg
     2881 ccatttgtag acaattccca tccgggactt tttgaccgta tgctgaccaa ccagacttcc
     2941 atgttcaact atcacagcgg cctggaggca acgcatcctt attcctcttc ctggtatcag
     3001 tggcccacta tggtccgccc gatctggtac tattccggat atctgaccga tgcagtcaaa
     3061 gagggaatca gtgctttcgg caatcctgtt gtatggtgga tcggtattct ggcatttatt
     3121 tacatcctgt acctgttaat tcgcaacaat gcgtttctct gttctctgac aggacacaca
     3181 caaacagagt gctccacaga tactgcgacc accctgtccc acagagaata tgcagcagct
     3241 gctttcctcg cagtcggcta cctggcgcag tacctgccct ggttcttcgt caccagaatt
     3301 accttcatct accactattt ccccagtgtt ccttttgtag tgctgatgat cgtgtacagc
     3361 ctgatgcagt ggaagaagca tatgtctgac cgcaccttca tcatcgtctg ctgcgcttat
     3421 gctgcggtgg cgtttgccct gttcctgctg ttctatccgg tattgtccgg gcagccggtg
     3481 gatgtggatt ttgtaataaa atacttgcgt tggcgggata cctgggtatt gatctcaggt
     3541 taagtcctct ttataaaata cttcatataa aaaacagtgc caatccaggc actgtttttt
     3601 ggtatagcat atctattcac ttacccctca gcgatcgcat taccggtata caactgataa
     3661 tatctgccct tctgggcgat gagttcatcg tggttgccgc gctcgatgat gcggccctgt
     3721 tccagaacca tgatgcagtc actgttcttg acagtagaaa gtctgtgggc gatgacgaaa
     3781 gtggttcttc ctttcatcaa cttatccatg ccgtcctgta caattttctc ggtacgggta
     3841 tcgatagagc tggtagcctc atccaggatc agtaccggag gatccgctac ggctgctctg
     3901 gcaatagaaa gcagctgtct ctgaccctgg cttaagttcg caccgtcacc ggtgaggact
     3961 gtgttgtaac catccggaag ctgacggata aaggtatccg cgttagccag ttttgctgcg
     4021 gcaatgactt cctcgtctgt agcatccagc ttaccgaaac gaatattgtc cataacggta
     4081 gcggtgaaca aatgggtatc ctgcaatacg atacccagag acttccgcag atccgctttt
     4141 ttgatcttgt tgatattgat gccgtcataa cgtatcttac catcctgaat atcatagaaa
     4201 cggttgatca ggttcgtgat cgtggtcttg ccggcaccgg tggagcctac gaatgcgatc
     4261 ttctgaccgg gctttgcata catctcgatg t
//
LOCUS       DAWDHD010000204         4211 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_1218_length_4211_cov_11.390760, whole genome shotgun sequence.
ACCESSION   DAWDHD010000204 DAWDHD010000000
VERSION     DAWDHD010000204.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4211)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4211)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4211
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1218_length_4211_cov_11.390760"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1228 a          846 c         1146 g          991 t
ORIGIN      
        1 aatttatcaa ccgtattgat gatattatcg tgttccacca gctgaacaat gagaacatga
       61 aggagatcgt gaatcttctg gcatccaatc tgtacaagag atgtgaggat cagctgggga
      121 tccatctgac catcacagca gcgctgaagg agcatctggt atccaaatat gcggataaca
      181 agatgggagc aagacccttg aagcgtgcga tccagtccgt ggtagaggat gctctggcag
      241 aggagatcct gcagaaaaag gtggtaccgg gagataaggt atccgccgga ttcaaggatg
      301 gaaaagttgt atttactgtg aaaaattaag tatttgtaac tattcagtca ctcaatttgc
      361 aaaatcgctc ttgcagagcg ttccttttgc tcattgaggt tacttcgtcc tataaattac
      421 gtcaaaggtt cttacgaatg caagcattcg acgtacctat gacacaattt acatgactct
      481 gaatagttac aaaataaata aaactttttg tagaaaaaca gggtgagata tattatactg
      541 taactattca aaccccattc gcaaagaacg aaagagaaag atgtattgcg gatgcagtta
      601 ctttcaggag gacagacaga tggcagtagt ggaagaaata cttcgcagtg aagcagacgg
      661 atctatcagc tttggtaatc acaagctggc caagaaggca aaagtggaag attatgagca
      721 tgcaggagat ctgctgaagg ttaagaccta caatgagatg acaaagctgg aaaagaacgg
      781 catgttttta tatgaatctg ttcccggaac cagtgtactg gagttcaagg aagctgacaa
      841 cagtgtggag ttcatcgtag aaggtgacga ggattcccag atcactgtag gactgaagga
      901 cgataccgag tacgaagtat ttatcgacgg taagaacgta ggaatcatga aaaccggact
      961 tggcggcaaa ctgagcctga gcgtagaact ggaagcagca ggtgaagtac ctgttaagat
     1021 cgtagaggcg taaggacgca ctgcaaagga gtccaggcac ggaggatatt gtagtacttc
     1081 gtgactgggc tttttttaaa tattcagagt ttccggaggc ggttttgcgg gggctgaata
     1141 ggtattacaa tgattgagga gagagcatgg caaaaggaaa gacggccact gtatttttct
     1201 gtcagaactg tggctatgaa tccacgaaat ggctgggaca atgtcccggc tgcagagagt
     1261 ggaatacttt cgtggaagag acgatagata aaacggaatt gaaaaatacc ggaaacggtg
     1321 caaagagaga caggagagag gcttcggagc cctgtgtgct gtcggagatc gctatccggg
     1381 aggaaaataa gactctcacc ggaattaagg aactggaccg ggtgctggga ggcggcattg
     1441 tgcagggatc tctgacgctg gtaggcggag accccggtat cggtaaatcc acactgttat
     1501 tgcaggtctg ccataactta tccacggcgg gacataaagt attgtatatt tccggtgagg
     1561 aatccaaggt acagatcaag atgcgggcgg accgtctggg ggctttttcg gagcatatgc
     1621 tgctactgtg tgagacgaat ctggatatca tcagtgaagt gatccgcaag agcaagccgg
     1681 aggtcgtgat catcgactcc atacagacca tgtacaacga gaacgtgtcg gcggcgccgg
     1741 gaagcgtgtc tcaggtgcgg gaatccaccg gtattttgct gcagctggca aagggactgg
     1801 gaatctcaat cttcattgta ggccatgtga cgaaggaagg tacggtggca ggtcccagag
     1861 tgctggaaca tatggtggat accgttcttt attttgaagg ggacagacat gcgtcctacc
     1921 gtattctgcg gggggtgaag aaccgttttg gatctacgaa cgagatcggt gttttcgaga
     1981 tgcgggaaac gggactggca gaggtcaaga atccttcgga atatatgctg aacggacggc
     2041 cggagaatgc cagcggctct gtggtggcgt gtaccatgga gggcaccaga ccgcttctga
     2101 tcgaattgca ggcactggtg tgccacagta atttcggcat ccccagaaga cagaccaccg
     2161 gtacggattt caaccgtgtg aacctgctga tggcggtatt ggagaagaga tcgggagtgc
     2221 agctgtcctc ctgcgatgcc tacgtgaata ttacgggagg tatcaagatc caggagccgg
     2281 ccattgatct gggaatcgtg ctggcgattc tgtccagctt ccggaataag gcattgaatc
     2341 cgaaaatggt agctttcgga gaagtgggat taagcggcga agtccgcgcg gtcagcatgg
     2401 caaaacagcg tgtggcggag gcggagaaac tgggcttcga ggaatgcgtg gtgccctacg
     2461 tgtcactgga agagtgcaaa aaaggaagca aaatccgtgt catcggcgtc agctccgtgc
     2521 aggatgccat ggaccgattg ttctaaagaa gctttttagc atttcattat gaggaggaca
     2581 aagatatggc cgtaagaata ggaattgata tcggcagcag taccaccaag atcgtagctt
     2641 ttgaaggaga acatatgctt ccgcccatgg cagtcaaggc agacagccag gtggcatccc
     2701 tctacggtgc tttcggaaga tatctgtatg agaataatct gcagctttta gatgtggaag
     2761 gtgtctatat caccggcgtg ggaagcaaat atgtggagaa agcggtatac gatcgtccga
     2821 cctataaggt agatgaattc gttgccacag gaaccggagg ctattatctg acggataaaa
     2881 aggaagttat cgttatcagt atgggaaccg gaagcttttt cgtcaaggtg acggagaatg
     2941 agatgaagca tttaggcggt gtcggcttag gaggcggaac catctgcgga ctgtcctcga
     3001 tcatactgaa taccggggac atccatgaga tcgtccctct gtcccggaaa ggtgatgtgg
     3061 caaagatcga cctgcggatc ggcgatctgg ccaaggaaaa gctccccggc ctgaacctgg
     3121 acgtgactgc gtccaacttc ggcaaagcgg atgcccagtc caagcccgag gacatcgcgg
     3181 ctggtatcgt ccatatggtc atcgagaaca tctgccaggc cggaatcctg gcctcccgga
     3241 acaccggcat caaggattat atcctcatcg gcggcctgac caaattcttc gagtgccgcc
     3301 agatcatcga agacttcaag agcctgtggg atgtaaagat tactattccg gagtattctg
     3361 attatgctac cgctattggg gcggtgattg cgccggatgc ggagaaagag gagatttgag
     3421 aaataaaaaa gtaagaggga ttgatttctc aatccctctt ggtgtataat ctatttagaa
     3481 agtaagtcgg aggtgaaaat tccgatttgc cctcagtaaa atactagaaa agaaatagca
     3541 tctactttgg cgaggggatg ctatttcttt ttgttattat cgtcaccatg tctgtcaatg
     3601 tatgataaag ctgtaaagac aactaacaac agagtaagta cttccatcgt attcatgggc
     3661 atcaccctcc tttgtaagac tagagggcat ctaacaatcg gaaaatcgca tccgctttct
     3721 gttcgattac acaaatacag tataacataa atcgaacata tgttcaatat catgatgaaa
     3781 aacattcctg ttaattcaca ttaaaataac accccatcat ggcgtgattc ttcagatcgc
     3841 gccatggtga ggtaaaaagt gttaccaaaa cagaaaataa aaaaagccga taacatcgac
     3901 ttttgaaagc aggggaagag gggatcgaac ccccgttaac ggttttggag accgccgcac
     3961 taccgctgta ctattcccct atgaaattgt atcagctgaa tcactgacga aagctattat
     4021 agcatagatg tttatcattt agcaagtgaa aattttaaat ttttgcaaaa acttttgcaa
     4081 ttccggggaa aaccacaaag aataccttat aacacaatta aagcagccgc aggcgggaaa
     4141 cctgcagcca ctttaattgt gtaaaagggg aattcttccg gaaatctact tatataagcg
     4201 gttcggctct c
//
LOCUS       DAWDHD010000205         4141 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_1257_length_4141_cov_9.799559, whole genome shotgun sequence.
ACCESSION   DAWDHD010000205 DAWDHD010000000
VERSION     DAWDHD010000205.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4141)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4141)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4141
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1257_length_4141_cov_9.799559"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          903 a         1194 c          845 g         1199 t
ORIGIN      
        1 atgtagcaga gcgtggtcag atttctgagc tgctgtatac tcattttctt tctcctctat
       61 atccctatcc tctgaaaaat ctgtccaaaa ttattatttc ttacaatgtt ccgcaatggc
      121 tgcgatcgct ttctccatct ggaacggttc cgccagatgt gcattccttc cggcggtcag
      181 acatttcttc gcatcttctt catacgtcag cgcattttcc ctattcctca cattacaaat
      241 ccatttttgc atttgaatgt atatttaatt tattttacca tgttaactgc cggacggcaa
      301 ccttattgct ccagcatatt ctcaataaaa atcccatatc ctctggtact gtcctgcacg
      361 agaacatggg tgacggcccc tacgaattta ccgttctgga tgataggtgc cccgctcatt
      421 ccctggacga ttcctccggt cagggcgatc aggtcagaat ctgtgacttt cagttcgatt
      481 ccccggttca cgttgtcatg atcgagatga atgtcggtga tctcgatatc atagtatctg
      541 gtggtgccgt ctacggtaca caaaatctgc gccggtccct ttcggatctc ctgttttaat
      601 ccaatgggca tcgcctcttc cgttcccatt gccaatgctt tgtcattgca cttgccgaag
      661 attccccgga cactgttgct ggtaatatcc cccagaatcc tgtcatcaga atagacgatc
      721 attcccgtca tttcccccgg atgaccggcc gttcccttct gaatggagat gatctccgtc
      781 tggtacaggg ttccgtcatt catttccatc aggttgctgg tgtccacatc attgattcca
      841 tgccccagtg ctccgaaatt tccctgactg tcaatgtagg tcatggttcc cacaccctgg
      901 gcattgtccc tgacccagac accaatctta tatttcttcg tctgatcctg taccggatcg
      961 attttaacat caaaatattc ttcgccccgc cggatcgtca aaatcgctgc ttctccacta
     1021 ccgttttcga tccgggtgat cagatcatcc ttgtccgcca cttccgtgcc gttcagtttc
     1081 accacataat cgccgctttt caaaatatgt tccgccggag ccaccttctc cccgttgaca
     1141 gaacggaact ctccggtacc tatgaccatt accccttcgg tcttgacata gattccgatg
     1201 ggaacaccta ccgggatcag ctgctgatcc tcaatcaccc ggatccccac ctgcttaaaa
     1261 ggcaggatcc cgaacagctt cacctgcatc tcaaggctgt ccgtcattcc ggtcttcata
     1321 gtcaccggct gactcagatc cagtgtcacg gcaccttggg gaatattact ggttccctgt
     1381 tcactgacac ttaaaatctc accttttgcg ggaatatcca gtgccagtga ttcctccgat
     1441 tccgcccgca tgtaaatgac agaaggaata ctgctgtcca ccagataata gctgtagcca
     1501 aagagtccca taccggccag gataagggca gcacaaaggc aggcccggta aatccgcagt
     1561 ctgcgcacct tctgtttcca gagctgcaaa atgatgactg catcttctcc tgcctgcctt
     1621 tttacttttt ccgtttccgt aatccatagc ttcatacaca ttcccccgct gaacttctgt
     1681 cattgctgta tagggtatag tgtacgtcac attcctattt ttaatctgtg atcaccggga
     1741 tttgcggttt acagttttct ccagtttaac cacaaatgac cacattcccg ctgcttttat
     1801 attcctccgg gcagtcgtcc gtcagaatcg ttccactctg aaacgccccg aaggtcacag
     1861 agctgacatt tccgaacttg gaatgatctg ccagaataaa acattctctg cattgtccca
     1921 tggcagtctt tttcaccatg gcctcattca tatccggtgt ggtcaggcca ttctgcctgc
     1981 taatcccatt ggtcccgaaa aagcctttgg taaaatgata actctgcaga ttcatcaaag
     2041 cctgattacc taccacggcc tccgtggagc cttttaattc accgccgacc agatatacac
     2101 ggaatcctgc cgctgccagt gtcttggcat gcgctaccgc attggtcaca aaggtggcac
     2161 ttttctctgt cagataagaa agcatacttg ctgtggtcgt acctgcatcc agatagataa
     2221 aatcctccgg ctggatcagt gctgccgcct tggcagccac cagctgtttg gcttccacat
     2281 tgacttctgc cttctgttcc accgtgggtt catgtgttat caccgtccgg tcacatgcca
     2341 cagcacctcc aaagaccttt accagcttcc cggcattatg caatgctgtg atgtcccggc
     2401 gtaccgtgga ttccgacgta tttaacagat cttttacctc tgccaccgta atgctgtttt
     2461 tttcttccaa taatttcaga atgatctcat aacgttgttc tgtcagcata aattgttatt
     2521 ttctctttcc tttgccacat ctgtctccag tatgatctta cgcacgggaa gaatacttcc
     2581 gggagacatg gataattcat caacacccat agccagaaaa cgtctggtca acgatgtatc
     2641 tgcacccagt tctccgcaga tacctgccca gattccggcc ttatgcgcat tttccacgac
     2701 catctgtatc atacgcagca ctgcgggatg atgcggatca taaaatgcat ccagcttcgg
     2761 attctgcctg tcgatggcca gtgtgtactg ggtcagatcg ttagtaccga tactgaagaa
     2821 atccacttcc ttcgccagct ctgcactcat catgacagca gccggtgtct cgatcataat
     2881 accctgcttc ggttctccga aagccacacc ctgttctgtc agttcctgct ttacctctgc
     2941 cacgatttcc ttgatctgat gcacttcctc cacacttata atcataggat acataatgga
     3001 gatattacca aatgcactgg cacggaacaa tgcacggagc tgtgtcttga agatctccgg
     3061 tctggtcaga cagatacgga tggcacggca tcccatggcc ggattttcct ccggctccat
     3121 ttcgaagtaa tcgcacttct tgtctgcacc gatgtccagt gtacggatga tcacaggctt
     3181 tcccgccatg gtctccgcca cagtgcggta gatctgaaac tgttcttcct cggtagggaa
     3241 ggtttctctc tccagatata aaaactcact gcggaacagg ccgatgccgc cggcatcgtt
     3301 ctgcaatacg gccgccagat ccttgctgtt acctatatta gcatagagca tgatcttctt
     3361 accgtcaata gtgatatttt cacgtccctt taaggtctgg agcagttctt tcttctcctg
     3421 ctcctcctgc tttagctgct gcattttcga cagagtatct tcatcgggat ccacatagat
     3481 gcagccgttt cgtccgtcca cgataccaag ttttccgtct atggcatcgg tcagagggat
     3541 atcggtgccg atcagtgccg gaatgctcat ggtgcgggcc aggatcgccg tatgggaatt
     3601 cacagatcca tgtaccgtta caaaggataa taccagatcc ttgttcaact gtaccgtctc
     3661 ggagggtgcc aggtcgtctg ccacaatgat catgggttct tccgatgccg ccatgcctgc
     3721 cgttctgcca cccagcacgc ttaatacgcg ctcggaaatg tctttgacat ccgcagatct
     3781 ggctctcatg tagtcatcct ccatggaggc aaacatctgt gcaaaattgt ctccggtgga
     3841 agccaccgca tactctgcat tcacctgctg catatgaatg atgttctcaa tggattcatt
     3901 gtaatcatca tcctccagca tcatctgatg aatctcgaaa atagctgcat tggcttctcc
     3961 cacttctttc aaggctttct ggtacagatt ctgcagctgc tccatggcgg tttgtctggc
     4021 ctgtctgtaa cgggcgatct cccggtcggg attctcgatc ttcacacgct ttacctgctg
     4081 ctcatccttc ttatgaacac ggatcctgcc caaggcaatt ccgccgaata cacttttccc
     4141 c
//
LOCUS       DAWDHD010000206         4107 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_1272_length_4107_cov_9.243583, whole genome shotgun sequence.
ACCESSION   DAWDHD010000206 DAWDHD010000000
VERSION     DAWDHD010000206.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4107)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4107)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4107
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1272_length_4107_cov_9.243583"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          983 a         1171 c          882 g         1071 t
ORIGIN      
        1 cagctgtgtc ttcggatccg ccgtctgtgc atggagaagg agctgaccgt ctttcagatc
       61 gtaccattcc tttttataat tggcattcca ctgccattgc agtcctaaac gatctccttc
      121 gaagaaatcg ctgtcctccg gggtatcgat gggatatgca gctcccactt ccggcttctt
      181 gtaacgcagt accggctcac cgcagccgtc cacggcattt actccgatca ccggccagtc
      241 gttttcccag tgcatgggct gtaaatgtac gatacgtccc gcattgccca catcctggaa
      301 atgcaggaac cagtcctgtc cgtccggggt atccacccag gcaccctggt gaggtccgtt
      361 gacaggagaa tttccctgat gcaggacgat tttctcttcc cagggaccgt aaatattttt
      421 cgaacgcagt acggtctgcc agccgggctt cacgccccct gccggagcga agatatagta
      481 atagccgttt ctcttgtaca gcttcggtcc ctcgatggtg ggctgggtcg catggccatc
      541 atagatgaac tgaccgtcat ccagcactcc ggagcagtcc ggttcgatgg gagacatata
      601 cagcatgctc ttgaaaccga tacggcttct ggcgaaagct gtcaccatgt atgctttgcc
      661 gtcctcatcc cagaaaggac agggatcgat ccatcccact accttacgca cataagccgg
      721 ctcgctccac ttgccgaaag gatccgtggt cttgcacatc atgatgccct cgtccggcat
      781 ggggataaat acgtaataag ttccctcatg ataacggatg gccggtgccc aggtaccaca
      841 accgtgtacc ggcttgtcat aatagtcaaa gggaagcttg tcaatgatgt aattgatgac
      901 cttccagttc accagatcct tcgaatgcag cagaggcacc gcaggggtgt tacagaagct
      961 ggaagcgatc ataaaataat cctctcccac acgaatggca tccgggtccg aataatcggt
     1021 atacaggatc gggttggtat aggtgccgtc gccctgatcg gcgatccaca ttttatgtat
     1081 cttggtcttg tccataattt ctccattccg cagcctttgc cgccttttat ttacgaatgc
     1141 aggtgttcaa cactcatttg ataatagtta cctgtttctg aacaatttca gtataacaga
     1201 tgttcccttg tatcgcgtgc cattttccca ttagtttgtg tcaaatcttg catttagcga
     1261 ctataaatgt cataatagtg acaaaagttg caggagggaa tcccattgag tttcttttac
     1321 cagaaccgtc aggaacagct ttatttatat ttatccaaaa acaaccccta tgccatccat
     1381 cttcacagac agttggagtt gatctatgta ttgagcggtt ccaccaccgt caccgtggaa
     1441 cagcaagatt ttttacttac ccccggtcag ggtgttctgg tttttccgaa ccagatgcat
     1501 ggtctgaagc atgtggaacc cggtcagaat ctgctctgta tcttcgagcc ggatttctgc
     1561 cactctttcc gccatttttt ccagaacaaa aagcctgccc gcaataactt taccgtctcc
     1621 tccctgtcag agcacagccg tattgccctg cagggactta aaacactggc cgctgccatg
     1681 gaaaaggatc gtcccacacc ttccgatgta caagtctatg cggaagggta tctcacactc
     1741 ctgctggcag acctgctgcc atcgttgcct ctgcaagtgc gtaaaaacac agatgacctg
     1801 gaattggagc aaagactgct tctctatctg gatacccact ataccgaaga attgtccctg
     1861 gaaagtctct cgaaagaatt cggtgtcagc cgctttgtcc tgtcccggat cttcactgag
     1921 aaactccaca ccacttttcc tgattacgtg aactcccgaa ggctggacta tgccagagat
     1981 cttctattgt ccacggagct ctccgtgacg cagattgcgc tggatgtggg attcggaagc
     2041 tcccgcacct ttttccgggc ctttcattcc gcttatcaca ccactcccgg agcttaccgc
     2101 aggcagaaag aatcttcctg atcccttccc ttacgctgta tccacagaag ttcctctccc
     2161 gcccggatat aatctttcca ggtgccgacg atctgtcccc gctccgggat ctggcacatt
     2221 tccaccgtca ccgccgtatc cacgccttcc cttgccaggc cctcctgatc aaattccatc
     2281 agcagttttc ctttgcgcac gatctcgttt tgcaggacat tgcaatgata ataggcgctg
     2341 tgcagttctt ccctgtctct gcagatattc aacagcagtt ccagtccgct gtccgtgcgg
     2401 aatcggatcc ggtttcccat gggatagagt ttcaatacct ttcctgccgt gggcgcatag
     2461 acctttccat cctctggcac gatgctgata cagccagttc cggcatcagc ggtctcttcc
     2521 gcggtctctt ccatgcgctt acggatctct ccgttcaccg ggctgcctat agtgacacct
     2581 tccctcacca gttgcctggc tgctgtattt tttctcgctt ccctgtcttc ccctgtctct
     2641 tctgtttctt ccattttcca ccggatactc ctgccagacc agaagccgac caacagtccc
     2701 agcactcccg ttgccacgat gtcatacatc tgcatgtttc taccttcctt tttccatgat
     2761 aaggttcccg ctccttattc tgtctcattt tcgggaaaac atgccatttt ttcattcata
     2821 acttccaaaa gaagtgccgc cacgattcct gccgccatgc agaacagatt ccgggatgcg
     2881 gtctccggag atgtggcaaa actgttccat ggaaccgtca tcactgtagc tgcactgatg
     2941 atcccgaaga tcccgtggaa catccccgca tactgttttt caagcaggcg gtttaccagc
     3001 ctggccagac acacaaaagt caccacgcag ccggtcattc cgggaatcaa cacctccgac
     3061 cgaagatgag cgatcccctc tacgaacggt gtatacagcc ctaagggcat cagtagtgta
     3121 gaaaaactca tccccggagc gatcaccgac agagcaaggg caaatccaca gaacagatac
     3181 gctccgagtc ccggctccac tgcagtcttg gaagtctgca acgaaaacag caaaaaaatc
     3241 attgccgcaa acgtcactcc ggagacgatc ctgtttcctc cggtcctgcc ctgctctccc
     3301 gcttcccgcc acagggacgg cagcattccg ccgatcagcc ccacaaatac acagacggaa
     3361 ggctccggat aagtctctaa tacgtaagat aacagattcg ccacccccag aaatcctgct
     3421 gccgatccaa tcatataagg gaataattcc ggcagatggg tccgccactt atgtaccgga
     3481 tccgacagca gctccatgat cggtcgatag actccgaaca ctacacttaa aacgccgccg
     3541 gatatccccg ggagtaccgc tcccagaccg atcagcgccc cttgtaatac ttttcccaaa
     3601 aatctaagat ccatcgttat tctccgcatc ctgttgctct taaaatcata tgctttttcc
     3661 ataagaaaat aggtctaaaa atcaaagata attattgaca tttcgatcag ggcaatggta
     3721 tactctaacc gtattctaaa tgaaagtcat tgatctcaca tatttttgta agacattgct
     3781 actttttcac ggatgcttta caaaaatatg tgattttttt gtcctacgtt ttagaatcat
     3841 caaaaggatc aggcaaagcc tgaattatta aaggaggtac caataatgaa taacggtaca
     3901 gttaagtggt tcaatgctca gaagggttat ggtttcatca ccaacgatgc taacggcgag
     3961 gaagtattcg tacacttttc cgcaatcaat gctgatggtt tcaagtctct ggaagagggc
     4021 cagaaggtaa ctttcgatac cgagtgcgat cctcagaaca gcagcaagat ccgtgctacc
     4081 aacgttactg ttgtagctta atagcaa
//
LOCUS       DAWDHD010000207         4086 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_1281_length_4086_cov_9.995287, whole genome shotgun sequence.
ACCESSION   DAWDHD010000207 DAWDHD010000000
VERSION     DAWDHD010000207.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4086)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4086)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4086
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1281_length_4086_cov_9.995287"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          978 a         1106 c          729 g         1273 t
ORIGIN      
        1 tcgtgtatct gaaaagaagt aaagccttcc ggggatttta aaagacaata tttgttttct
       61 ccgctacgga aatcgcattc caacagttcc ataaactttt tcatgattct tccttttcat
      121 tctctgaaag cacgatcacc agccgctttg gcggcaatgt atccgcatcg ggattatcct
      181 ccataccgat tccatatacg ggcctgtcat cgtatttctc cggtatgtca tctctgtact
      241 tatacatttc accggaccag ttgtctctgt ccactacaga aaaatatccg aatatctgta
      301 aataaggttt caaatcccga aacaaaagct ttccttccgt actccgggca atatctcggg
      361 gtttctgata caatacgatc gccacccggt cgatccggta aggaacacgt cttccgtttt
      421 cttctgtgaa aaatctttct ttaaagattc tttccagcaa aagttctctg gcatctttcc
      481 agtatccctt cccacgaaac tccggataca gcaccggaga tatcttacag gtattttctt
      541 tgacttccaa cgcaacataa ccgggaatca gcctcttatg tttacttccc tcaaggaacc
      601 gctctttttc atcctcttct tccaactgtg tcagttctac cttaagatcc tccctgtaga
      661 aataattcct gtacataaat atcctcgtcc cgttttcagg cgtaaaatcg ctcttttgtc
      721 tccagacaga gacaggcaag tcttccaccg gcaaagccac atccgcaatc taagagatat
      781 acgttatgtc tgtcgttgca ccagatggaa ttgcccggct cgtccagata tcttctgtcc
      841 ttcttccaca caatgctgtc cgtgggcgta tgaccgcaca tggagatata tccatcgata
      901 ccttcaaaga aaaagcagtc aatatccatc gtcccccgca tgtaatatcg gtcctcttcc
      961 cggacatccg gatgggaagt catggcatgg gcaaacagat atttctttcc ctccagctcc
     1021 agttccttct gtagcggcag tcccttgatc agatcagcaa tgttccgcat gtctacttcg
     1081 gtaatacgct gtcgcagcag ttcaaaactg ttgtaaccat agggagctaa tttgtgccgt
     1141 tttttctccg gagtattaaa gtactcataa gtataatttg ccagccagtc atcgtggttg
     1201 cctcgtatcc aggagcactt tccctgcaag ccggcaaggg cacaatatac tccgaagggg
     1261 tccgcagttt ctccaccccg gtcaaacaca tcaccaagga cgatcagttc atcatccctg
     1321 gtgatattga tctgctttag gatctgtttc agtttcttca ggtcgttatg tatgtcgccg
     1381 ataacataat gcatatcctt cctccttttc tcatttcctt cggtagcact tctgatatct
     1441 gtcgagcttt ctggaagcgg gatcgctcct tgcttctgtt ccccttcgcc tcatataccg
     1501 gcgtatgctt atagcttctg ctcattcaga ctccttaata tactttgtcc catatagttt
     1561 ctgcctcacc cgcgaacccg gtgtgctctc tgcttctcat cctgcacctc ttccatctcc
     1621 ggaaaatctg cctccagata caggggattc ggaaaaactg atattcaaag cggtcctttc
     1681 tctgggatcc acccgctcct cttcctccgc aggaatctcg caagaatccc atgtcagaca
     1741 ttctccaccg gctttatgca tgctgttccc actgcttatt cctatgcatt cctccctgcc
     1801 gggaagttct ggctgtaata gtcctcaaat ttcacctgaa atacaattct ggaccgcata
     1861 ccgagatttc tgtcaaggaa cccctgcata ggttccgggt acccggcaaa tatcacaatg
     1921 acatcctctc tgtggttttc catttcccga agcaatgtcg taatagcgat ctcgccttgc
     1981 agagaatatg cttcatcaat gaataatacg cctccttttg ctacttcccg gatttcctcc
     2041 gaatatcata tgcatcatag atgcctgata atgctttcct ccccgtctct tcctctcttt
     2101 ctccacaaca tctatggcaa tatttttgta aataagtggt agtatattta gaaaataaaa
     2161 tgatttttgg atatggggat atggataagg ttataaaaaa tttctggcta cataaaacga
     2221 gacggccgca accgtctcat ttaaagtctt acttttaaaa agtatcatta tttgtattat
     2281 aggagctata ttcgcaagtg aatgaatatc tattcaggct ttcaatttgt ctctttcttc
     2341 aatccttttt gtaaccgtgg aaaaatattc catccaatca gcatccgttg cagatgctaa
     2401 attgctgtaa ccgtaactcc aaaatgggat tatttcctta cgcggatcct tatccacaaa
     2461 ataacactca aatttattgg tctttacata atcaacccac gctttttctt cttttatata
     2521 cttctcatgt atgtttttca gttttgcatc ccaattatcc aaggcaccac ccctggatgt
     2581 caccgctgca cttgtcatat tgcccacggc ataaactgat ccaaggaacc ccataaatgc
     2641 atctataacg tctttttcta ttttttcccc tgttttttca tgccgaattt ttgataagct
     2701 ttctcggagt tgctcttcat ccttccccat aggaatccgt tccaataaga cttcctccaa
     2761 ttctttcctt gatttttgca ttaactcctt cttatttccc aataaaaaga ttttagtcgg
     2821 agtccaccag cttgtcatga tatcatttct catcttttca tcactctgct tcggcatcag
     2881 tgaagcatct gctacgctat tttcaacatc tccaggatat aatttagaaa acataagaga
     2941 attatataac atattattgt tgtcccaatc cttcgacagc ttcacaaatt cgtccagtaa
     3001 tgtccttcta cagtctttca tctagatctt ctctttcatc gtaatcaccc tttagaagtc
     3061 cccgaaccag atattcagat ccacttcctc attaataccg tccacagcac ctttttcgct
     3121 gtactgccat accttatagg catacggata atacagatca tcattataat aagcgaacca
     3181 cttatcgtac tgttccagct gtcccagatc cagcatcagt gttcccatct ccatgttgtg
     3241 atagatcatg ggcttgtagc cggcatcctg gatggtctgg cagaacaggg cggtcaggcg
     3301 ggttctctct tccacgctca gttcgttggc acggcccttg ccgccgctga ctttttccac
     3361 gtcgaagacg atgggcagtt cgatgttata aggttttacc ttttccagta ccagattggc
     3421 ttcttccaac agttcctcgt ctgtgatggc ctgagagtag aagtagacac ctactttgat
     3481 tcctgcctgt aatgctccct tgacattctg ctcaaagtat tcgtcctcca ccagcttacc
     3541 ctcagtgccg tagcctctga ggcctacacg gatgaatgcg aattccacac cgtctgcggc
     3601 taccttggtc cagtcgatat tgccctgatg cttggagacg tcgattccct tgtgggatac
     3661 tacctgaccg tcctgcatat actgcacttc tccgtcctcg agaatgttca ggttctccaa
     3721 tacataatca tttttctgca aatcatcacg aatgggtacg aaattaaagg ttccgttgct
     3781 cacaacgacc agttcattgg gatagtaagg acgcagggtc tctaccatgg tagtgccgga
     3841 ggtcagacca tcacggatcc cggagaggat cttgttctct tcctcgtcct cagcctgctg
     3901 ctttgcttca gcgatcaggg catccacttc ctcctgcgta taggtcttca ccacttcctg
     3961 tgtgctctct tccgtctggc ttccggcttc cgctgctgcc gacagatcgt tggctatatt
     4021 ctggaacttg ctgacgatcc aggaatacag gattcccgca acatacgtta ccagaacaaa
     4081 caccaa
//
LOCUS       DAWDHD010000208         4001 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_1324_length_4001_cov_11.165737, whole genome shotgun sequence.
ACCESSION   DAWDHD010000208 DAWDHD010000000
VERSION     DAWDHD010000208.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4001)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4001)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4001
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1324_length_4001_cov_11.165737"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          931 a         1175 c          826 g         1069 t
ORIGIN      
        1 ccggcgggga tcggatccgt agaagctgtc tttacattgc tgttccggcg tctcctgccg
       61 gaggccaaag ccctgtctgc cgtgcttctc taccgtttcg ccaccatgct gttaccttgt
      121 gcgatcggtg cgttctttgt actgggggag aggattattt ctctgcacag gaaaaagaga
      181 acggcagact aatatgcttt cctaaaatct ccacacaaaa caagccacta cacagtattc
      241 tgtgtcgtgg ctcttattat gttccgttat tataaaaaca aatgctaagt attactttct
      301 ctgtaagaaa tcaggaatct tcagagattt ctcttctaca gagcttctga tatctgtagg
      361 cttatgaata ccgggcaccg gctgtatggg tctcggagcc tgtcctccct gtccctggga
      421 tgtcgtagga gcggtagact gtatattcat tcccttcaga gaactgggag tcatatggga
      481 agcaggctgt ctgaatgcag cgcctcctcc cagacgattc tgtacaccgt tgttggcctt
      541 gtcttccaga ccggtagcaa taaccgtaat ggtgcaactg tcggacttgg actcatcata
      601 catggcaccg aagataatat ttacatcgtc tcctgccagc tcctgaacat agcttgccgc
      661 atcggaagca tctgccaggg taatgtcacc ggaaatattg atgataacat gggtagcacc
      721 agaaatcgta gtctctaaca gagggctctc cactgccatc ttgacagctt ccatagcctt
      781 gtcatcgccc ttgccttcac cgataccgat gtgagcaata cccttgtcct tcataaccgt
      841 ctgtacatct gcgaaatcca gattgataac tgcgggaaca ttgatcaggt cagtgattcc
      901 ctgtactgcc tgctgcagta cttcatccgc cttcttcagt gcttccggca tggtggtacg
      961 tctgtccacg atctccagaa gcttatcatt agggattacg atcagggtat ccacgtgttc
     1021 cttgatacgc tcgataccgt ttaatgcatt gaccatacgg gcttttgctt caaaacggaa
     1081 aggcttcgtt accacaccaa cggttaagat gcccatatcc tttgcaagct tggccactac
     1141 cggagcagct ccggttccgg tacctccgcc cataccacag gtgacgaata ccatgtctgc
     1201 acccttcaga gctgcggaaa tctcttctgc actctcctcg gctgctttct caccgatctc
     1261 aggcttggct cccgcaccaa gacccttggt cagcttctca ccgatctgta acagtttcgg
     1321 agctttgcag agctgcaatg cctgcttatc ggtattcacc ccgataaaat ccactccgtc
     1381 aatcttctca tctatcattc tatttacagc gttgttaccc gcgccaccta caccgactac
     1441 gatgatcttg gcagctacat ccgcgtcatt cgtcttaatc tccagcaagg ttagttcctc
     1501 cttttaaccc ttttcaactt cacatagact atcatataat cattttacaa atttagcaac
     1561 ctattttttc aaaatgtttc acaaatgttt cacgcgtccg gtttgaatgt aaatttaccc
     1621 tttccttcct cgtagttttc catttgcagg gttccgcttt taccctgtaa cttctccagg
     1681 aagtccggca acaactgcag cttgtcctct atatggctgt tgtcactgcc catggacacc
     1741 ttgatctgtc cgaagtagat cgtaatgtca ccggaagaat gcaaatagat cttgtccgca
     1801 gccagtgaat atttgttcaa aagctttgtc agatccatga tcctggaaaa cacctgcgga
     1861 tcctccaccg gcagttgctc tcccagcacc acatggttga aggaaagccc cgtgatctgc
     1921 ggtattccct gggtgcggat cccggaactt tccaccacat agccgtcttt atcaaaatac
     1981 atataagtat ccagatattt cacatatccc gccagtgctt tttcatatac agtgatcttg
     2041 atcgtgtcgg gtgccagaat cgatacattc atcacatcca caaagggaat gtcctgaatc
     2101 cctctgtccc ggtatttcag agacagatac agggaattat ctcccaaagg tcccgacatt
     2161 acgatctcca tgatctcgtc ctcagtataa tgcacattgc cctccacata cacggtacgt
     2221 accgtataca cggagtgcaa tgccaccgca ccaccgccca gtgccagcag caggatgcct
     2281 atgatcacta ttcttctgat aattttgctt cggtctctat acactgatga tccgtgctcc
     2341 taattctctc aagtctctgc agatattttc atatccgcgt tctataaagt gctgtcctgt
     2401 cacgaaactc tcgccttctg ccgcaagccc cgccagaacc agtgccgcac cgccgcgaag
     2461 ttcacaggct tccatccggc ttccccgtaa atgctccact ccggtgatca cggcatgtct
     2521 tccgtccttc agaacaatct ctgctcccat gccctttagc ggttccgcaa tacgaaaacg
     2581 attctcgaaa atattctctt caatgcagct gcatccttcc gctttcgtca gcaccgccat
     2641 tgccaccgac tgcagatccg tgggaaaccc cgggtaaggt ccggtccgta aatacggtat
     2701 ggcctgcggt cttttggaac actgtacgaa cagaccttcc tccgaggtga tacactgtgc
     2761 acccattttt tccgccacaa aggtcaccgc ttccatctgc tcccagggag cctctgtcaa
     2821 aagtacactg cctccacagc cgatacaggc gaacagatat gtcccggcta caatccggtc
     2881 tgacggaact gtgtaccgtg tactatgaag tggtctgtca ccatgaacca gaagttccga
     2941 tcctccaatc ccctcaatct gtgcaccaca tgccgtcaga aattcgcaca atgccgtcac
     3001 ttccggttct ctggcggccc ccagtatcct ggtgtctcca tccgccttca ccgcagccag
     3061 caaaatattc tccgtagctc ccacactggg aaactccagt gtgatctctg ccccgtgcat
     3121 tttcccatcc ttcacctgtg cagacagaag cccttcttcc tcccggaatt cgacgcccat
     3181 ctgctgtagt gccctgatgt gcagatcgat cggccgttct ccgatgacgc acccacccgg
     3241 atgctccatg gtcacataac cacatcttcc cagcaatgct cccaacaggc acagagatga
     3301 acgcattccg gtgatagctt ccccctgcat ccgtctggcc tccgctctct cgcagggaag
     3361 ttcttcattt tctcccggct gtacccggat ccctttttcc tgccagtgca ccagacatcc
     3421 cagttcccga agcagatgca caaaacgatg tacatctgca atacgtgggc agttctgaat
     3481 ataggatact tcattggtaa gaagcgttgc cgccaggatc ggaagtgccg cattctttga
     3541 tccctgtatg cgtaccttcc cctgcagcgg aatgcctccc tgcatgcgga ttcctttcat
     3601 acagcaccat ccccgttttt atgataatct tatcttatgt aggaaagcac tgtttcgtac
     3661 catgtcttct gaatatgtat ttttctacag ctcttttaac tggatccggc gcgccacgct
     3721 taataccagt ccgatctccg acagcaaaaa caatgccgaa gaaccaccgt aactgataaa
     3781 cggcagggaa attccggtgt tgggaatgga gttggtaact acggcaatgt ttaagatcgc
     3841 ctggatcgcg atatgtccca taacgcccac caccagcatg gcgccgaaca ggtccggtgc
     3901 attgttggca atgaccatca ttcgccataa gagcataatg aacatgagaa taatggcaac
     3961 cgctccgaac aatcccagtt cctcgcagat aatggagaag a
//
LOCUS       DAWDHD010000209         3964 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_1342_length_3964_cov_11.007930, whole genome shotgun sequence.
ACCESSION   DAWDHD010000209 DAWDHD010000000
VERSION     DAWDHD010000209.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3964)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3964)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3964
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1342_length_3964_cov_11.007930"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          962 a          989 c          910 g         1103 t
ORIGIN      
        1 cctccggttg atgggtgttc tcattcttaa ggttccgtcg aacctatttt accaaactta
       61 cagtctctct ggcaatagca agttcctcat tcgtaggaat taccataaca gtggtcttgc
      121 tgtcaggagt ggaaatagcg atctcctcgc cacgcttgtg gtttgcctcc tcatcgatgg
      181 atactcccaa aaagcccaga tgttcgcata ccagggaccg aactaaaggt gcgttctcgc
      241 cgatacctgc ggtaaagcag atcacatcta caccgttcat ggctgcaaca taagagccga
      301 tatattttgc cacacggtag cagtaagtgt tcatggtacg gatgcagtgt gcatcgccgt
      361 tgttgtaacc tgcttccaga tcacggaaat cggaagacag attgttggac agaccatata
      421 caccggactt cttgttcaga acggtcatga tctcgtcgat gttcttgccc tctttgtgag
      481 cgatgaactc catgatggca ggatcgatat caccggaacg ggttcccatg atcagacctt
      541 ccagaggtgt cagacccatg gaggtgtcta cacacttgcc gttcttaaca gcggatacgc
      601 tggcaccatt gcccaggtgg caaacgatga tcttcagatc ttcgtacttt ttgcccagaa
      661 cctcagcagc cttcttggat acataagagt ggctggttcc gtggaagccg taacgtctga
      721 tcttgtattt ctcataatac tcgtaaggca gaccgtacat ataagcttcc tcaggcatgg
      781 tttgatggaa agcggtgtca aatacagcta ccatgggagt ggtgggcatc agcttcttgc
      841 atgcattgat accgatcagg ttagcagggt tatgtaaagg agccaggtcg ttgcattcct
      901 cgatggcttt cataacttca tcggtgataa tggtggatgc tgcgaacttc tcgccgccgt
      961 gtacgatacg atgtcctacg gcaccgatct catccagact ctttaccaca cctgtctttt
     1021 cattggtcaa agcttccagt accagacgga tagcttcggt atgatccggc atgggagtta
     1081 cagtctgctc tttctcacca ccatcggggg tataggtgat ctggcttcct tcgataccga
     1141 ttctctcaca aagacccttt gcgatgcatt tctcggtctc ggaatcaatg agctggaact
     1201 tcagggaaga gcttccgcag ttaataacta aaatattcat gatgaattcc tttctttcaa
     1261 atctgttact ctatggggtc atgttacccg tgcgggcaat acatgaatac ggtgcgggac
     1321 agatatcccg cacacgttta tgttcgttac cagtgattat actaactgtg cctgtacagc
     1381 ggtcagtgct acgacaccga cgatatcctg ccaggaacat cctctggaca gatcgttaac
     1441 aggctttgcg ataccctgta acatagggcc gtaagcttct gctccaccca gtctctgtac
     1501 cagcttgtaa ccgatgttac cagcatccag gttcgggaag atcaggacat tggcatgtcc
     1561 gtttacaggg cttccgggag ccttagactt ggcaacgctg ggaaccagag ctgcatccag
     1621 ctgcagttcg ccgtccagag tcagatgagg atactcctca tgtgcgatac gggtagcttc
     1681 tactactttg tcaaccagtg cgtgctttgc acttcccttg gtggagtggc tcagcatggc
     1741 gatcacgggc ttaggtccga tcagagactt gaagcttctg gaagaagtat ctgcgatagc
     1801 agccagttct tcgggagtag ggtcctggtt cagaccacag tcagcgaaca ggaaggttcc
     1861 gttctcaccc tgatccttga atacggtatc cataacgaag aatgcggata ccagtttaac
     1921 gccgggtgca gtcttcagga tctgcagtgc aggtcttaaa gtatctgcgg tagagtggca
     1981 tgcacctgct accataccgt ctgcatcatt agccttaacc attacgatac cgaaggtcag
     2041 gtaatcattc agcaggatct ctcttgcctt ctcgggggtc attccctttg cttttctggt
     2101 ttcatataat aaattcacat agtcatccaa cttgggagtt gttttagggt taactacctt
     2161 aactttggac aggtcaactt ccagccagcc tgcaccatcc atgatcttct cttcatcacc
     2221 gatcataatg atgtctgcga tgccctcttc cacgatacgg gcagcagcca gtaatgttct
     2281 cttatcatta gattccggta atacaatagt ctttttgtcg agtcttgctc tctctttaat
     2341 catgtcaatg tatgacatat ccattctcct ttccggcggg gcatacccgc ttcctcccaa
     2401 ataacttctg taaagttttg gttttatctt aacaaaccga tgtatttata ttatactaaa
     2461 tacatctgga gtacacaagg gcataatgtt cgcaaaattg tatattttca gatacaacca
     2521 ctattttttc agaataaaat aagctgataa ccatgcatat gtaaacgctt tcagtgtatg
     2581 ttattttttt cacattcctt gtcagggttt gtgttcgtta taacgtatat ttcttttcac
     2641 gaaaggagcc acattatgaa ggtcaatggt attgtcgcag agtataatcc cttccataat
     2701 ggtcatgctt accagttaca gcatgccaaa gaggccaatg atgccgatta tacgatcatt
     2761 gtcatgagcg gtaatttcat gcagcggggt gctcctgccc tcttagataa gttcacccgt
     2821 acaaaaatgg ctctggagaa cggtgcagat ctggtgctgg aactgcccac ccgctatgct
     2881 gcctccagtg cggaattttt tgccaagggc tcggttgcct tgttcgacaa actgggagtg
     2941 accacaaatc tgtgctttgg cagtgagtgc ggcaacattg aagtcttgtc acggatcgct
     3001 gagattttct atacggaacc ggaaccttac gtggaatcct tgcgctgcaa tatgcgtaag
     3061 ggaatgtcct tccccatcgc caggacctgg gcgttattgc aatacgcgcc ttccttaagc
     3121 gatgataagg acgttctttc ctctcctaat aatatattgg gaattgaata tctcaaagct
     3181 ctgatgtcca gaaacagcaa gatctcaccg ttcactacca ccagagtcgg tgcggattat
     3241 cacgacaaac gtctcggcac caatcagtgt tccgccattg ctatccgcca gtcggtggcc
     3301 gccgggcatg atctatccta tctggctgcc cagatgccgg aaagtgccta tgcattgatg
     3361 acggattctc tgtctcacaa aaagcctctg tttgccgatg atttttccgc agcactgcaa
     3421 tacaagctgc tgacggaata ctctgtgggc tacgacaagt atcaggatat ttcttcggat
     3481 ctgtccgacc ggatccgcaa taccctgcct tccttcgcag gttacacttc tttctgtgat
     3541 ctgctcaaga gtaaggatat gacctacacc cgcatcagca gatgtctgct tcatattttg
     3601 ctgaatatga ccaaggaaga attcgagacc tgcaagtcgg aagactatat ctcttacgcc
     3661 agagtgttgg gtttccgcaa gaatgcgacg ccactgcttg ccgagatcaa aaagaacagt
     3721 tccattcctc tcgtcaccag cctggctgac gccagacaga cgctttctcc cgaatctctg
     3781 cggatgctgg atctggatat cctccgcaat cagatctatc tggggaatct ggccttaaag
     3841 aaccagacgg aaatggtgaa tgaatatcgg acgccaattg tgattgtata aatggaaatt
     3901 tagcgaaata tccccgtagc ccttggcagg gtataagaaa cgcggcacgc tcgcgctctg
     3961 tcgc
//
LOCUS       DAWDHD010000210         3956 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_1345_length_3956_cov_8.698539, whole genome shotgun sequence.
ACCESSION   DAWDHD010000210 DAWDHD010000000
VERSION     DAWDHD010000210.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3956)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3956)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3956
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1345_length_3956_cov_8.698539"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1139 a          756 c         1125 g          936 t
ORIGIN      
        1 ttggtaaaat aggttcgacg gaaccttaag aatgagaaca cccatcaacc ggaggatccg
       61 attggtgggt gttttacgta tgcaaatcga gcatgagaaa tccccgtcga tgtatggtaa
      121 cgaatcaaca gagggagaaa aattatgaaa aaaaatgtga tcgtaggaca gtccggaggc
      181 cccacagcgg tgatcaatgc cagtctggca ggtgtctaca agacagcaaa ggatatggga
      241 gcggatacca tatacggtat gcgctatggc attcagggtt tgttggagaa aaagatcgtg
      301 gatctggggg aaaagatccg caacgacatg gatgtggaac ttttgaagag aacaccggct
      361 tcctttctgg gatcctgccg ctataagctg ccggagatca gtgaagataa agctatttac
      421 gagaaaatat ttgcaattct ggaagaactg gagatcatgg catttttcta tatcggcggc
      481 aatgactcca tggataccat caaaaagctt tccgattatg cagagacagt gggcaacccg
      541 attcgtttta tcggcgtacc caagaccatt gataacgact tagagggtac ggatcatacg
      601 cctggttacg gaagtgcggc aaaatatatt gctaccgtta ccaaggaact ggtacgtgac
      661 ggcctgatct atgagatgca gagtgtgact gtcatcgaga tcatgggaag aaatgccgga
      721 tggctcaccg gagcagcagt actggccaag agtgaagact gtgaaggccc ggatctgatc
      781 tacctgccgg aaatagcctt tgatgtggat gattttctga aaaaggtcaa gaaactggtc
      841 agcgagaaac agtccattgt cattgccgtg tccgaaggaa tcaagacggc agacggaaga
      901 tatgtctgcg aattgtctgc accttccgac aagacggatt ctttcggcca tgtacagtta
      961 tccggtacgg gcaaatattt gtctgacctg attattgaaa agctgggctg caaatccaga
     1021 gctattgaac tgtctactct gcagcgctgt gccggacatc tgacctcccg cgtggatatc
     1081 accgaggctt atcaggtagg tggaactgcg gtaaaggcag cttttgaagg caagaccggt
     1141 cagatggtga tcttaaagag agtatctcag gatccctata tctgcactac ggatctctat
     1201 gatgtgcata aggtggcaaa tctggagaaa aaggttcccc gttcctggat cacggaagag
     1261 ggagattatg tgaccgcaga gatgaagaac tatatcgagc ctctgatcca ggcggaactg
     1321 actccgatca tgatcacagg ccagcccaga cacattatta ttgacgatct gtaaaagaaa
     1381 atggaataaa acaacaaatc aaataacgat acaaaggagg tggaaagatg ccgaaacaca
     1441 ggaagagaag aatcatgctc agcatagtga gtattgtgct ttttgtagtt ttatctgcgc
     1501 agctttccac cttttctttt tctgtgactg cggccgggga acgcggagaa ctggtggcgg
     1561 aggagtacga acgataccgg gaacgctttg acaggataga aaagattgga gatttggagg
     1621 accagggatt ccgcctgctg gaggatcaga tctttgcgat gccgttgcaa aaacttccgg
     1681 aggaagccgt agatacgaca gaggaacccg gggatgaagt ctggttctat gcggcactgg
     1741 atacaaggta ccacagactg gcggtattta tcgcggatgc ttccgggcag attctgtaca
     1801 agacggacca gctggaagca aattactgct atccgggaga aatgcggcag ccggtgaaaa
     1861 aactggcttc ggtttctttt caggatgtag acaatgacag agatacagat atcatcctga
     1921 tcgtccagtg ccataatgac aggggggatt atcaggaaga atcctacaaa gtaggagatg
     1981 ttctgttcca ggacgatgga aacttttacc gtgattatcg gatctcagac aagatcaacc
     2041 gctttgatat gaacaagaac cctgcctgta ttttaaattt tgtaagagac gggcgttcta
     2101 cggaattcct ctatacggcg gagacactgg cagatcttct gaaccacaat ttcaatgtca
     2161 tcgaggaaca gtcttatacc cgtaattttg aaaagctggg aaaattaaag gtggttccag
     2221 gcacctaccg gatggcggaa tatgacgtgt tcatgatcta tctgatcgat gaacagggaa
     2281 atattgtgtg gagttttcaa cccatggagg attacgacaa tctctatgcg ctgaagggaa
     2341 tccagggaaa ggacctggac ggtgacggcc tgaaggatct ggtggtgttt gcaaaataca
     2401 gctatgaagg agaaaacggg gaattgttgg tggacaccgt gtgcaccatt tattaccaac
     2461 ggacggccgg atttgaaaaa gacaaagatt ttaccgcaaa ttatgaatgt acggaggagg
     2521 acaccttaga ggcactggtt gggaagatac gtgcctactg ggggtggcag acataatgat
     2581 aaaaatattg atcgtggatg atgaaaagcc gatctgtgat ctgatcgatc tgaatctgtc
     2641 agcagcagga tatttttgca aaagcgtaca ggacggcatg aaagcaattg aatgcatcga
     2701 gagagaaaaa atcgatctga tcttactgga tattatgctt cccggagtgg atggctttga
     2761 catcatggaa tatatcagac ctttgaagat cccggtgatc ttcattacag ccaggggaga
     2821 tgtgaaggac cgggtaaagg ggctgcggct tggtgcagag gattatctgg tgaagccctt
     2881 tgatgtcgtg gaacttctgg cgagagtgga ggtcgtatta cgcagattcc ataagacggg
     2941 agcacagttg tccgccggtg atgtggtggt tgatatggat gcccgaatgg tgaccaaggc
     3001 aggaattccc gtggtgctga ccaataagga gtatggactt ttggtgttgt ttatccagaa
     3061 caaaaatatt gccctgttca aggaaaatct gtatgaaaaa gtgtggggcg aagaatatga
     3121 cggagatagc agaaccttag aacttcatgt gcagcgcctg cgcaagaaga tgggatggga
     3181 gcgtcagctg gtggcggtct ataaggtggg ataccggctg gagatcgcat gacccggagt
     3241 gggtaaaagg agagatgtat gaaattttcc tggaaaatac tgttttgtac gatgttgatc
     3301 atggcagcgg catgcggagc aagcggttat ttttttgcaa attatgtgtt tcagacctcc
     3361 atggagcgtg agaccagaca ggcacttgat gaaagtagta ttttgaggtt cgcttttgag
     3421 acggcagctt taaatattcc gtccaaatat gatgtacttc cggacggaac agtggaacag
     3481 atcggagtat acctggagaa cagtggacag cacggcaaca gattgctgcg gatcagcgat
     3541 gagaacggta aggtgctgta tgtcagcgaa gggtttaccg gggatacttc tctgtgggaa
     3601 gaacgcggag aaaatacgag agtgtaccgg gtagtgcagt cgggggattc ctattatatc
     3661 cagacacaga caaggatcaa tgtgtccgac cgaatgctta cgttagagac catgaaaaat
     3721 gtaactgcgg tatatacgga gaggacggaa ggatttcgga tgtatcgtca ggtgacggtg
     3781 atcgtattgt tatgcagtgg agcggtcatg acgctgattg cctgttggct gaccaggcct
     3841 atcagacttt taacccatgc caccggcaaa atggcggagg gagaatacag ctatcgcgcg
     3901 gagcagatca gtaatgatga aatggggcag ctgaccgcgg ttttcaacca tatggc
//
LOCUS       DAWDHD010000211         3942 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_1353_length_3942_cov_13.057885, whole genome shotgun sequence.
ACCESSION   DAWDHD010000211 DAWDHD010000000
VERSION     DAWDHD010000211.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3942)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3942)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3942
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1353_length_3942_cov_13.057885"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          966 a         1064 c          803 g         1109 t
ORIGIN      
        1 ccggtggagc ctacgaatgc gatcttctga ccgggctttg catacatctc gatgttatgc
       61 agcacgatct tgtcatcatt gtaaccgaag tccacatcgt cgaataccac ttcaccctgt
      121 aattcaatat aatctacaga tccatccgcc tgatgtacat gcttccatgc ccacataccg
      181 gttctctcgg tacactccac gatcttgccg tttaccttct tggcacggac gatggtcaca
      241 tatccctcgt cggtctctac cggctcatct agcagatcga agactctctt ggcaccagcc
      301 tgtgccataa cgatactgtt cagctgctgg ctgacctggc tgataggcat gttgaagctc
      361 ttgttaaaag ttaagaagct ggcaagctta cccaacgtca gtcctgcgaa accacccagc
      421 gccatggcac caccgacgat ggcacatacc acatagctta agttacccag ctgggcattg
      481 atgggtccca tcaggtttgc aaacttattc gcattgtatg cgctgtcgca cagttcatca
      541 ttcagagcct tgaactcctc cagactctta tcttcatggc agaatacctt gactaccttc
      601 tggccttcca ccatttcctc aatgtaaccg ttgactttac cgatattctt ctgctgcttt
      661 acgaaatatt ttccgcttaa gcccgctacc ttaccagtga cgaacatcat cagtgccacc
      721 atgatcagac tgactgctgt cagaggcaca cttaatacca gcatacttcc cagggtaccg
      781 actacggtga tgaagctgga aaatacctgc gggatactct ggctgatcat ctgacgtaaa
      841 gtatcaatat cattggtgta aatggacatg atatcgccgt gggaatgggt atcaaaatac
      901 ttgatcggca gcttttccat gtgttcgaac agatcatcac ggagattctt cagtgttccc
      961 tgagttatgg ttaccatgat acggttgtaa gcataagtgg ccgccacacc gaccagataa
     1021 aaaccagcca cacggacaat ttcccggaac agattgccaa agtccggagt ctcctgtccg
     1081 atcatgggca tgatatagtc gtcgatcagt ctctgcatga acagagtacc ctgaatgctt
     1141 gccacaacgc tcagtacaat acagaccaac acaaagataa agtgaatgcc gtaattttta
     1201 aagatatacg ccatcagtcg tttaaatact tttcccggat tctcaatacg tgtaccgccc
     1261 tgcattcttc tgccgggtcc acgcattccg ccgcccatag gtcctggcat tatgactcac
     1321 ctccattctc atcaaagtcg ccgccacccg tctgggtctg ggcttcatat acttccttat
     1381 agatctcatt agtacgaagc agttcttcgt gaggtgcaaa tgcatcgatc ttaccgttat
     1441 ccataacgat gatacgatct gcatcctgaa tactggagat acgctgtgca atgatgattt
     1501 tcgtcgtacc ggggatctcc gtagcaaatg ccttacggat cttcgcatcg gtagcggtat
     1561 ccacggcact ggtagagtca tccagaatca gtaccttcgg ctttttcagc agtgctctgg
     1621 caatacacag tctctgcttc tgtccgccgg atacgttact tccgccctgt tcaatatagg
     1681 tattgtattt ctctggcatc ttctcaatga attcatcggc gcaggccaga cgacaggcac
     1741 ggatgcattc ctcttcagta gcattctcgt cgccccaacg gagattctct aagatggtac
     1801 cggagaacag cacgttcttc tgtaatacta cggatacttc gttacgcaga gtctccagat
     1861 catagcttct gacatcctta ccgccgacat agaccgtacc ctcatttaca tcatacagac
     1921 ggctgatgag gttcaccaga ctggtcttgg aactaccggt accacccaga ataccgatgg
     1981 tctctccggc tttgatctca aagttcaaat catccagtac cggcttctca ctctgtttgt
     2041 tatagcggaa ggtcacatga tcaaaacgga tactaccgtc tgtcacatcg tagtcaggtt
     2101 gttcggggtt ggtgatgtcc gccttttcat ttaatacttc cgcgatacgc tttccgctgg
     2161 cagcgctcat ggtcatcata acgaagatca tggctaacat catcaggttc atcagaatcg
     2221 tcatacaata ggtcagcagg ctcatcagtt caccggtagt caactggctg gcaacgatca
     2281 tgtgagcgcc aagccagctg attccaagga tacagccgta tacggtaaac tgcattaccg
     2341 gccatacata agtcatggta cattctgctt ttttgaacat cttgtagatg ttgtagctgg
     2401 ccttatggaa cttgtcgatc tcatagtcct cacgcacata agcctttacc acacgctgtg
     2461 cggctacgtt ctcctgtacg ctggcattta aatcatcgta tttcttgaat acttcactga
     2521 aatacttgga cactgctctc ataataaaaa tgataacgat tccaaggaac actaccgcca
     2581 ccagataaat acttgccact cttgcattaa tcaggaacgc catcagcata gcacagatca
     2641 ggctgaccgg tgcgcggaag cacatacgta agagcatctg gtaagcgttc tggatattgg
     2701 taacatccgt ggtcatacgg gtgaccagtc ctgccgtgga gaacttatca atgttggaga
     2761 aagagaaggt ctgaatgttc tcatacatgg cctctcttaa atttctggca aaaccggtgg
     2821 atgctttcgc accgtattta ccacccatga caccggcgaa caatccgaca atagctgcca
     2881 ggatcatgta acaaccgatg gcaaaaatat gtttcatatc tcccgcctgc acaccgtcat
     2941 caatgatgga tgccatcagg agcggaatca ccatttccat ggctacttcc aggatcatga
     3001 aaataggagt tacaagagat gcactcttat attccttgat ctgttttgct aatgttttga
     3061 tcatagctgg tttcctttct tgtatttttc tatcctataa taaatgtttt gtaacctctc
     3121 tgttatgcat gactgccagc atcactcgtt ccggcatggc ccgttacaac gtccggtccg
     3181 cctgctgtcc tgccccagat tctgcatcat cttatctaaa atagttaaga atgtctgctt
     3241 ctcttcttcg gtaataccgt tctccagctc tcgattgaaa tcccggatct gctcttcgat
     3301 cagattgtga tgctcgatcg ctttctccgt caacaggatc cttttcagtc gggcatcctc
     3361 cgtggatgcc acccggacga tatagccctt ctgttccaga ttttggagca ttaccgttgc
     3421 cgtggatctg cggattcgga attccttctc cacatccttc tgatatacat cgccgttccg
     3481 gctcttgtga tacagatatc ccaacagcgg cccctgcatt ccgttgattt catttccttc
     3541 catctgagca aagatcgcat cgatcttccg gcggatcatg ttgttggcgg ccttgatctc
     3601 aaatccaagc tgacggttcg tcagtacact tgtctcctcg ttcatcctgc ctccttatct
     3661 cctatgcctc taatgcggtg ttcgtcaaat cctcatgcca gcatgggatt ttcctctcta
     3721 ctcgcgcaaa ctatgagaaa cacgcttcgc gagtttctca agttatcgcg gcagtcgcca
     3781 aggtctcgtg caagcacgga ctttggctcg ttgctcgcgt aaataaaaat gttagatagc
     3841 taaccatttt tgttagccat ctaacattat aacgcaatct tttatgtttg caagaaaaaa
     3901 agtataaact ctttataaac tccgtcaatc ctccagtacc cg
//
LOCUS       DAWDHD010000212         3916 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_1365_length_3916_cov_12.554520, whole genome shotgun sequence.
ACCESSION   DAWDHD010000212 DAWDHD010000000
VERSION     DAWDHD010000212.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3916)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3916)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3916
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1365_length_3916_cov_12.554520"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1153 a          859 c         1042 g          862 t
ORIGIN      
        1 aggccatgga ggacggagtg gatgtgatcg ttttctctgc ggtggattac aatgccaatg
       61 cggagatcat cggtcaggca gcccagagag gaattaagat cgttgtcatt gacagtgatg
      121 tcaacagcag tatggtaagc tgccgcattg ggaccaataa cctgcaggcc ggtgtgaagg
      181 cggcggaagc agctctggcg gtgggagaag acgaactgta tatcggtatc gtgaattatg
      241 atgtgaattc cgccaacggg cagcaacggg aactgggatt ccggcagacg gtggagcagg
      301 acccgagagt aaaggatatt accaccatca atgtcctttc caccacggag gatgccagag
      361 cgggagcaaa agagatgctt ttgtcggatt ccaggatcaa tgtggtggtg acctttaacg
      421 aatggaccag cctcggtgtg ggctgggcca tccgggatct ggaccgggga gacaggacgc
      481 aggtggtggc gtttgactcc aatgtggtct ctgtgggaat gttggagacc ggggaggtgg
      541 atgccctgat cgtgcagaac ccctatgcca tggggtatct gggagtggag acggcggcga
      601 acctgatcaa cggacagacc ggcggcccca gcgtgataga cacagccacc actattgtga
      661 ccagagagaa catgtacgag cgggagagcc agaagatatt gttctctttt gaagagagaa
      721 agtaactatt cagaacccca ctcgcaaaac cgctctttca gagctattct tttgctcatg
      781 tggttacttc gccgaataag cattacataa ttcttacgaa tgcaagcgtt cgacgaaatt
      841 atgtaatgct tttcggctct gaatagttac aaattatgta cagaacatga acaaaatgtg
      901 tacagaatag tatatttttt aaccccatgc gtaagatttt gtatatcttt tatgtacaag
      961 atgccatata attacctcat caagagatga aacacataca ttttcatcaa aacaaggagg
     1021 tatttcatat gaaaaagttc atgtcactac tgttggtcgg cattatgatg ctcagcctcg
     1081 tagcgtgcgg aaagagcggc ggaggaaagg gagagttgaa tgtaggagta ttctattaca
     1141 cctacagtga tacatacatc tccagcgtgc gtaccgcact ggataatgcg cttaccgaag
     1201 caggcatcaa gttccagaat tatgacggca acagtaacca gaccacccag aacgagcaga
     1261 tcgataccgc tattgcacag ggtaccaacc ttctgatcgt caacatcgta acatccggtt
     1321 ccgtagacgc ttcccaggcc atcgttgaca aggcaagcgc agcaggaatc cctgtaatct
     1381 tcttcaaccg tgcggtagaa tccgatgagg atgaaggtaa ggtattaggc agctatgaca
     1441 agtgtgcatt cgttggaacc gatgctcccg aagcaggtca tatgcagggt aagatggttg
     1501 gtcagtatgt agtagacaac ttcgatgcta ttgatctgaa tggcgatggc aagatcagct
     1561 acgcaatgtt catgggtcag ttaggtaacg tagaagcaat ctatcgtacc cagtacggcg
     1621 tagaagatgc agatgcagtg atcaccgcag caggcaagcc cgctctggag tacttcgatg
     1681 catccaatac cgataagtat caggttgacc aggacggcaa ctggtccgca accgcagcta
     1741 acaactacat gaccaccaac ctgtctcagt acaatgagtc cgctggtaac atgatcgagc
     1801 tggttatctg caacaacgac ggtatggctg aaggcgttat ctccgctctg aatgacaagg
     1861 gctacaactt aggcgatggc aactgcacca ccattcctgt attcggtgtt gatgcaactg
     1921 acgcagccaa gcagctgatc gcagacggca agatgaccgg taccatcaag caggatgcag
     1981 aaggtatggc aaacggtatt gcttacctgg ctaagaacat ccaggctggc aaggatctga
     2041 tggcagatac cgacagcttc aacatctctg agaaggttag caacaagatc tacatccctt
     2101 acgctactta caccggagaa tgatctgaat aacaagtaaa taaaagaagc ggctgctgca
     2161 tctggcagcg gccgctcttt atgtatagga ggatgcgtat gggacaggaa gtattacttc
     2221 agatgacaga catatgcaag gaattccccg gcgtaaaggc actggatcat gtatccctta
     2281 ccgtaaaaaa aggaacggta cacgcgctca tgggagagaa cggcgccggt aaatccacct
     2341 taatgaagtg tctgttcggc atctacagta aggatgcagg caagatcgaa ctggaaggca
     2401 aggaagtcaa tttcaaaaat tccagagaag cactgaataa tggagtggcc atggttcacc
     2461 aggaactgaa tcaggcactg aagagaaatg tcatggataa tatctggctg ggccgttatc
     2521 ccatgacagc aggcattatg gtcaacgagc acaagatgct ggaagacacc aacaagattt
     2581 ttgaagaact ggggatcgct gtagacccca agagaatcat gagtacgatg ccggtatcac
     2641 aaaggcagat ggtagagatc gcgaaagcgg tatcctacaa cagtaagatc attgtatttg
     2701 acgaacctac ttcatccctg acggaagaag aagtggagca tctgttcaag atcatcaata
     2761 tgcttcgtga cagaggatgc ggtattatct atatttctca caagatggca gagatcctgc
     2821 ggatcgcaga tgaagtcacc gtcatgcgtg acggtacctg gatcgcaaca gagcctgcac
     2881 agaacctgac tacagacaag atcatcaaac tgatggtagg ccgtgaactg accaaccagt
     2941 tcccgcccaa gaccaacaag cccggtgaag tggcattgga ggtagaacac ttaagcgcga
     3001 gatattcctt gctgcaggat gtatccttta aggctcgaaa gggtgagatc gtaggtctgg
     3061 ccggactgga cggcagcggc agaacggaga cactggagaa tatcttcggt gtggccacca
     3121 gaaaagacgg tgtgatcaaa ctggacggca aagaggtcag aaaccgcaat gccagagagt
     3181 ccattaaaaa cggttttgca cttctgacgg aggaacgtcg tgccaccggt atcttcggaa
     3241 tcctgaatat ccgggagaac acggtgatct ccagtctgaa gaaacataaa aaagcaggat
     3301 tttatctgag tgataaatcc atggagaagg acactgcatg ggccattgat gccatgcata
     3361 tcaagacacc ttcccagaag acgaagatcc ggtcactgtc cggtggtaac cagcagaagg
     3421 ttatcctcgg cagatggctt ctcacagagc cggaggtgct gatgctggat gagcctacca
     3481 gaggtatcga cgtaggtgca aagtacgaga tctaccagct gatcatcgat ctggccaata
     3541 agggcaaagt tgtcctgatg gtatcttcgg agatgccgga gctgctgggt gtatgtgaca
     3601 gaattcttgt catgagcggc ggtaagctgg caggagaagt agatgccaaa aatacgacac
     3661 aggaagagat catgtctctt gctgcaaaat atgtatagga ggatatgcgt atgaatcccg
     3721 gcaaagcaat tcagaataaa attaacggcg taaagcaaaa ttttgcagac tacaagaagc
     3781 tggacagcag agacaagaag acctattgga aagaattttt acttaacaat gccttataca
     3841 tcctgctgat cgtagcaatt atttacactt acatccagaa ctccaatttc ctgtcggcgg
     3901 cttccattgt caacat
//
LOCUS       DAWDHD010000213         3900 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_1367_length_3900_cov_14.505852, whole genome shotgun sequence.
ACCESSION   DAWDHD010000213 DAWDHD010000000
VERSION     DAWDHD010000213.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3900)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3900)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3900
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1367_length_3900_cov_14.505852"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1201 a          823 c          904 g          972 t
ORIGIN      
        1 cctgtccatc agtgagaaga tgagaaagca gatcagaggt ctgacacagg cttccctgaa
       61 tgcacaggat ggtatcagtg cagtacagac cgctgaaggc gctctgactg aagtacacga
      121 tatgctgcag agaatgaatg agctggcagt taaggcatct aacggaacta actctgagga
      181 tgacagaaat tatattcagg atgaaatcga tcagttggta accgagattg accgtgtcgc
      241 tgagacaacc aagttcaatg agacttatct gttaaagggt gacaacacca agacttccag
      301 cgtatatagc tataacggaa ctcaggctat ggttacagat atggatggaa atgaacttac
      361 caacctgatg gacgtatgtg gtaataactt atccgcttct gctgcaaaga gtatgattga
      421 aaaaggcgaa aaggtatata agaccgctac aaacgctaca gcagctacag cagatgcagc
      481 atcagatact gagtataaga agttgactgg ctctttatat gatgcaaatg gtcatgaagt
      541 atacaaaaaa ggtgatatct taaagacaga tgtgagcact gcaaaactgt atacagatag
      601 taatgctacg aatgctgctg gtaatacagt agctgacatg accacaggaa cagaggcagt
      661 aaaagttgca aaagtagatc tgtataatgg agacggtacg gtggcattta agaaaggtgc
      721 tgaaatgact gcagcacagt ttggtaattt gactacaaag tactatacag cagttacttt
      781 gggcaatgaa gcagtagaag gaaccgactt caagattacc gagccgaatc tgtatgataa
      841 agatggcaat gcaatcgcag cgaatggtct ggagagctac tttgagacag acgatgacgg
      901 aaaggtaacc tacaagggtg gtctgtatga ttctaacggt aaagcggttg gcactaaggg
      961 caatacctta caggaagacg atgcgctgag taaattcatc ggcaaatctg agacaggtaa
     1021 gggcgcactc agcttgaatc tgcatgttgg cgctgatgcg accagcaaca accagattgg
     1081 tttggaaatc gaagctatgg atgcaactac tcttggcatc aaaggtatca aggtaaacgg
     1141 tgcagatgat acaaattctc tgagcgctat cgataccatt aaggctgcaa tccagaaggt
     1201 atccacccag cgttccgcac ttggtgctgt ccagaaccgt ctggagcata ccatcaataa
     1261 cctcgacaac gtagttgaga ataccaccag cgcagagagc cagatccgtg ataccgatat
     1321 ggctactgag atggttaagt actccaacaa caacatcctg gctcaggcag gtcaggctat
     1381 gctggctcag tccaatcagg ctaaccaggg cgtactgtct ctgttacagt aagcttaagc
     1441 tgagatcagg ttgtttccta agaggaacga accatatctg taagagttat gtaagaacca
     1501 aagggcagtc catgatgggc tgcccttttt gggttcttat aaggcctttt gatcatccaa
     1561 agatttcttt tccaattcct tcgaaaaatt atcatagtaa tccatggctg cctgtgtaga
     1621 aatagaaagc tcagcatcat actcaaaaaa gttcatatca atgtgcccct tttacataat
     1681 tttttgtgct tttgagaatt cattttcaaa ttcctttgaa aatttttcgt aatagtcaat
     1741 ggcagattgt gtagggattg atagtagagt ctctaacttg tggattgcat cctcttttga
     1801 caagttaaag tccttacatg accgaacaat ttcttttgct ctgccttcct ctacgccatc
     1861 cttgtaaata actctctttt ccgcctcagc atcatactca aaaaacacca tatcaataac
     1921 ctccttctta ttctcaagca aaaattcttt caaaatatcc ctcctgatac atacgtccac
     1981 ggcctgaacg atggcctgcc ggtttggata cacctttgcc tgctcacgga cttctgcgac
     2041 aaactgggcg tactccttta aaatctggca ggcatccatc agctcggagt tgaacccgag
     2101 attgatattg attacctgta cctgtaattc aagctgagga gtctcttcct tattccggta
     2161 ggcgctggac agcttcaggg tgctgtattc cgggagagga tccgtcccgt tataaaacac
     2221 tacgaaatgc ggagtatcca gcatctgtaa cttgttggaa taaagcaggt catctgagtt
     2281 catctttgaa tattcacttg ccagatacag caaaaagcga tacggcatat tgggattcag
     2341 ggtggactgg tgctcaaaaa accatagatc aaaatccacc acaaatgaca ggtcgttctg
     2401 caagcttagg taaaccgcat tttccaaagt tgtaatatgc agtagctccg gatcttcata
     2461 atgcgagtga ttcagcgcat tgtacagcga gagcagcttc tccttgtcat gaaatatttt
     2521 ccggaacacc agatccttat aattccgctt tccggaagcc ggtattgcca cagccacatt
     2581 ccccggcgca gcagctatcg cattcccata cgaaatttct tccgcaacaa aatttttcca
     2641 gaatctcgac acagtattca tcaaatactc ctccgagaaa tagttgctgg ttatattatt
     2701 ttgttttaac tccataaatc tcctttctgt tgcaaaataa atgtaaaaat aaaacaaata
     2761 ttttccatat aaaataaata ataagggaaa ctttgaccac tgacgggtct ataagaattt
     2821 ttgaaagaag aaaaattact tctaatacca ccataccata gggattcctt attggcaagt
     2881 ctgaaaaatg ttacaaaaag gctacaaaaa atccagaaaa atgttacaga acgcttctaa
     2941 agttttactt tcccggcatt ttggggtaag ataagcagta taggttccta aggacacaga
     3001 aagagaggat tactataatt atgagatttc aggacatgcc ttacgagaga atagattttg
     3061 ccaaagtgca ggaagagatg ggagaactga tccgggagct ggatgcggcg aagagcgggg
     3121 aggaacagtt cgaggtgcat aagaaatatt atgcgctgac ggatcgtgtc atgacgctga
     3181 tgaccattgc gaacatccgc tttgacattg atacttccga caagttttat gaggaagagc
     3241 ataaatacta cgatgagcag gggccggtat tcagcaatct ggtgctggct tatcagaaga
     3301 aattatatga atccccctac agagcttatc tggaggagaa gatcggtccg gtagcgttca
     3361 agaatatgga gctggcacag aagtccatga gcgaggcgct gatcccgctg atgcaggagg
     3421 agaatgctct gaccatggag tatgataaac tcattgccag tgcgaagatc gacttcgatg
     3481 gcaaggaact gaacctgtcc ttgttacggc cctatctggt aaactctgac cgcagcgtcc
     3541 gcagacaggc atgggagaag atgagcgcgt ttttcctggg aaatgaggaa cagctggata
     3601 ccatctacga taaactggtg aagaaccgta ccgcacaggc cagagccatg ggttacgaga
     3661 attacctgga gctgggatat taccgcatga accgcaactg ctacggcaag gacgaggtag
     3721 aagctttccg caggcagatc aaggagtatt tcgtaccctt tgcagagaag ctgcatgacc
     3781 gcagaagaca acgtctgggt ctggagaagc tcagctatat cgacgagcag gtatatttca
     3841 aggatggcaa cccggcaccc accggaaccc ccgaagagat catggcagcc ggacagaaga
//
LOCUS       DAWDHD010000214         3897 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_1370_length_3897_cov_10.915409, whole genome shotgun sequence.
ACCESSION   DAWDHD010000214 DAWDHD010000000
VERSION     DAWDHD010000214.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3897)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3897)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3897
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1370_length_3897_cov_10.915409"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          866 a         1120 c          812 g         1099 t
ORIGIN      
        1 agtcatcatt aggacttact ttccggatct cccgggtcat ataatatttt gtgatcacta
       61 ccgctgccag attgaacaat gcggtggtga tggccatggc cgctttcgcc ccgatcagcc
      121 aggcggaaag tctggctgtc cagaacagga tcggataggg aaactcataa tttcccgcaa
      181 tacctgccac cttattcaca tagtccggca tatccgactg aaagcccggc acctgatagc
      241 actccatcca aaacagatat aacataaccc cggtatacaa aaacagcagg aaaagaaatg
      301 tcacttttcc tgccattgtc tcccgcaggc cgctctttat gggattcttt ttcatagagc
      361 cgatcctttc tagttgaaac tgatcacggg atctttggga gcctttgcct tctccacact
      421 gatggcatgg agtacagggc cctctccctt atagtctgcg aagtattctc tggcaacctt
      481 cgctgtcact ttgacccatt ccttctgctt caggtctttc gtgcctgcaa actcacaggc
      541 atagcccaga aatgccatat cttccgcaca gcaggtcatt gccatgcgtc ccggtacgaa
      601 atagccatcg ggatattcct cgggcttcag taccatggcc agaaactgga tatttttgcc
      661 ttcatagcgc tccagatgat ccatggaatc cagataccag atgccgtagc cctggttgtc
      721 cagtacgata ggatcctgat tcagatcgta gggcagatcc tcctcgaaga tctcatcgat
      781 ctcaccgttg gaatcctcga agatcacatc tgcggaagga ttcaccgctt tgatgttgcg
      841 cttgtataca ttcagatcct tgataccgtc gcaacggttg aaaatgatca tctcggactt
      901 acggatcatc tctgccagca gagatttcat atttgtgtag tacataggga aggtggagcc
      961 atcaatggtc gtgatctgct gctctacttt ccagtaccag ggaagcgtca tgttcttaca
     1021 attccacata ccgttatact cgatgatgat acgggcgggc ttgtactgct tctccaactc
     1081 gatcagatga ttcggagtaa actcctcttc ctcttcgatc aatacgacat ccgtacgact
     1141 cttttttaat aaagcgggat cgtattcatt ctcgccctct tcacagagga tgagcagtgt
     1201 ctttcctttg atctggaaat acggctgtcc cagtgtaaat gtgataaatt ctgtcttacc
     1261 gctctccaga aaaccattta tgatatagac tggtttcgtc ataattcata cctttctctt
     1321 tcttatcctg ctgcctgtaa aatccgttcc ggcaatttat ttacggaaca gttctgccag
     1381 cttgtcctcg tccagtttgg aaccgatgac acagatctta ccggtaactt ccggcttacc
     1441 gcttctgatg tcatgctctt cgggaacata atcataatat acccaggttc cgtcgggagc
     1501 gggtaccata cccttggcac gcaggattgc accgtactct ccgctgtcca gagcagtaag
     1561 gatcttctcg atgtcctctg cgctgtaggt gttgggtgtc tcgatacccc aactggtgaa
     1621 tacttcgtct gcgtgatggt gatgatggtg gtggtcatga tcatgatcgt ggcagccgca
     1681 ggtgcagttc tcgtcatgct catgatgatg ctcatggtca tggtcttcat catggtcatg
     1741 atgatggtga tgctcatgct cgtcttcatc atcgtcgtca tcgtggtgat gtccacagca
     1801 gcactcatgg tcgtggtcat cctcgtcatg gtcgtgatgg tgatgatgct cgtggtcaca
     1861 gcactcatcc tcatcgtgat cgtcatggat atgctccagc atttccttca tcatatcttc
     1921 cagcttgtca gcgccttcga tggtcttcag tacttccctg ccatccagct gatcaagagg
     1981 agtggtgata atggtagcct tgtcgttatg ctcacggatc atctccacgc aggcattcag
     2041 ctttgcctga tccactttgt cggttctgct taagataatg gttccggcgt actcgatctg
     2101 gttgctgaag aattcgccga agttcttcat atacatcttg cacttggatg catctactac
     2161 tgctaccgca ctgttgatct ccacatccag atctcctgcc acgttctcca cagccttcag
     2221 gacatcggac agcttgccga caccggaggg ctcgatcaga atacgctcgg gagcataagt
     2281 gctgataact tccttcaggg aagtaccgaa atcacctacc agagagcagc agatacagcc
     2341 ggagttcatc tctttgatct cgataccggc ctcttttaag aaaccgccgt cgataccgat
     2401 ctcaccaaac tcattctcga tcagaacggt cttgctgccg tccagagctt cttttaacag
     2461 cttcttgatc agggtggtct taccggcacc caggaatccg gatactacat ctattttcgt
     2521 cattgttttt tcctccaatc tgagtctaag acccatcctt tttattttct atcatattta
     2581 cagtattgtc aataaaacga ttattaaatt tttatcaact aagccataat gttcgtcaga
     2641 cactgtgcgg atgctcgcat acgcaactgt gctacggtct ctccgtctcg atcatgctgt
     2701 aaggcaccac ggccactttt ccctcttcat caccgccaca caggattcct acgaacacac
     2761 cgtcctccgt aaaggctccg cctcctgaca ttccgggagt gatcagtccc tgcagcagaa
     2821 gcatatactg gtcaaaatcc tccacataga tccagggatc caccacgaaa gcatagaccg
     2881 gcagcccact ctcccctccg tacatccata catcgtcata ttccttcagt gcatcgaata
     2941 cttcccgatc cgtctgcacc ggcagacaga ccttccatgc ttcctctgac agttccgcct
     3001 tgtccaccca caggaaagcc agatcgcagc tgtccactgt ctccacgccg gtggcagcca
     3061 cctgtgttcc gtccggaaat gtcacccggg cctctccggt atcgtccgcc agtacgtgcc
     3121 ctgccgttgc gatcagaatc ctgttttcct cttcatcata gatcacaccg ctgccctgcc
     3181 gctctcccgt ctgaattccc accagggatt tcattacctt aaaatcattc tccgtcagtt
     3241 tctgctctgt ctgcaccggc agttccacaa attccattcc tgcgtcagcc tgtcccatgc
     3301 cactgttttc ttccgaaacc ttctggcctg cggtctccgt ggagacattt tcctcactct
     3361 caccgttttc cccgcagccg gcgaaaagta acaggcagct cagtatcata gtgatccctt
     3421 ttatccgcat tctctctttt ttctccatga cttctccata gtttctccgt gaaacatgct
     3481 ctgcattaca gtcgcttata acagaaaacg gaccgcatcc gcagtccgca tttttctcaa
     3541 aaggtgtaag caaaaaataa gccgagttct gtatatgacg atcatctatc tagtacgact
     3601 gtcaccagcc gcatcaagcg acctacctga aagcagaccg ggccggtcta tagctctctg
     3661 tttggtcttg cttcggatgg ggtttacatg gctccgtctg ttaccagccg gacggtagtc
     3721 tcttacactg cctttccacc cttaccggcc aaaagccggc ggtatatctc tgttgcacta
     3781 gccttggagt cacctccacc agacgttatc tggcatcctg ccctgtgaag cccggacttt
     3841 cctcacctgc ataaagcagc cgcgatcatc tatcttactt acacctgttc gattata
//
LOCUS       DAWDHD010000215         3854 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_1390_length_3854_cov_14.277441, whole genome shotgun sequence.
ACCESSION   DAWDHD010000215 DAWDHD010000000
VERSION     DAWDHD010000215.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3854)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3854)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3854
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1390_length_3854_cov_14.277441"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1131 a          799 c         1019 g          905 t
ORIGIN      
        1 tttcgggaaa agaaaaagcg aggaagcgta aaacatgaac gttatagaca ttatgaagcg
       61 ccctgcggcc tatgccagtg gctatgaaaa tatcccggga gaggaacaga accgggcaaa
      121 acagctttgc tgggaatata accggacagc tccgaaggaa cagcagaagc ggcgggaaat
      181 cttacaggag cttttgggaa cctgtcatcc gatgacaggc attgaaccgg attttcactg
      241 cgattacgga tttaatattc atacccacgg acttacagtc atcaattata actgtgtcat
      301 tctggatacc tcacccgtca atatcggtgc gggtgcattt atcgcgcccg gcgtctgtct
      361 ggcctgctcc ggacatgcgg ttgacctggg acagcgcagt cagggaatcg gtacttctgc
      421 acccatcacg ctggaagaaa atgtatggat cggggcgaat accacagtct gcggcggtgt
      481 gactatcgga gcaggatctg tgattggagc cggaagtgta gtcactcatg atattccggc
      541 gggagtgatt gcagcaggcg ttccctgccg ggtgatccgt cccattacgg agaaagataa
      601 gatccggccg gaggacattc ttttctaaaa aagatgacga tagccccact cttatgttat
      661 tatggtggaa aataatacaa aggagtgggg agaatatgtt tacaaaaagg agttgggaat
      721 ccttttattc cggtctcatt atggtgggac tggtatttat ggtgttcccg gttatttatt
      781 tctgcggtgc gacaaaggag aagcagatcc tgccgccgct tatcatgttt ctgctgggtc
      841 tggtgatctt cgtgattagt gttcactttt ctgtcaaata ccacaaggag aaaacgaaat
      901 acaccaaata taagatcgac aagtctctga cctacacatt caccttaact tacggggata
      961 aggtggagac cgtcacggat ttttcccaga tcgaatatgc catcaagtgg ctggcgaaat
     1021 accggacggg ccatgtggag atcgcagtca cgcctactat gggaagcctg aaaaagttct
     1081 acatctgcta caccgacaaa gatccgtaca ttcacatgct gatcgagcag gagagagaag
     1141 acggcgtggg atattgggaa gaacggggag attcttacaa gattcccatt atgacactcg
     1201 agcggctcta tgtgaaacat aagatgattt cgtttgatta cctgaaccgc agggagactg
     1261 gtgtggatgc aagggaggga ctgattttat gatgcagcgc tggcgggaaa aactgaaata
     1321 tatagaagcc ggaatgatca tcagtgcgtt gatccttttt gtggcaacgt tgatattcgg
     1381 agacaacctt acgctggaag tgatcgcgtt tatagtagga accatgttgt tttcccgtgt
     1441 gatcgagcgt atggataaaa aagaaatcga gaaaatgaaa tgccaggaag aggaagaact
     1501 gaaagatatt gtctgagaat aaatcctgtt tcccaaagga agaatgattg acacggaggt
     1561 tagcgagtgc taaccttagg aagagatttt gaaaactttg ggagtgacaa tatatggatc
     1621 tggcattctg gctggcgatt gcagcagcga tcctgtgcgt tgctgtcgcc atttacggtg
     1681 aaagtaaaaa ggataaaaaa tagggaaaag aatgacactt cagcaattaa agtatgtaat
     1741 aacggtggca gagaccggca ccatcacgga agcagccaac aggctgtatg tttcacagcc
     1801 cagtttaacc aacgcgatcc acgagttgga aaaggaaatg aatattgtta ttttcaaccg
     1861 gaccaacaag gggatcacgc tttccaaaga gggagatgac tttttgggat atgcgagaca
     1921 ggtgctggag caggcggcga tccttgagga taaatacaag ggaaatcccg ggggcaaaaa
     1981 acagttctgc gtatccaccc agcattattc ctttgccgtc aacgcattcg tggacctgat
     2041 caaacagtac gggcaggaag aatacgattt cagcctgcgg gagacacaga cctatgagat
     2101 catagaagat gtggcgcgtc tgcgcagcga gatcggaatc ctgtttttga atgatttcaa
     2161 tgaagcggta ctcagcaagc tgttgaaatc ccatgatctg gaattccacc agttgtttgt
     2221 tgccagtccc catgtattta tcagcagaaa acatccgctg gcgggtaaca gcataattac
     2281 gaacgaagaa ctggaagaat acccgtacct gtcttttgaa cagggagagc acaattcctt
     2341 ttatttttca gaggaaatct tctctgattt tgaacgcagg aaaaatatcc gtgtgcggga
     2401 cagagcgaca ctgttcaatc tgctgatcgg cctgaacggt tacaccgtgt gcagcggcgt
     2461 gatcgataaa aaattaaacg gaaaagatat tatcgccgtg ccactggcag atgagagtga
     2521 catgcggatc ggttatatca cacacagaaa aggaatgctc agcagactgg gtactaccta
     2581 cctggaagca ctgcagaagt atttaggatg aatgggttgt ccatagcttt aaactatgga
     2641 caacccttat ttttacgtat tttgcggatt tcatcatttc tcttataatg aggacaattc
     2701 aaaaaacgac aaagaaatgg agagaatgac aatgagcaat ttacagacac cttttcgcta
     2761 tgatttcgta ggaagttttt taagaccgga gaagttgaag aaagcaagaa gacagttcaa
     2821 tgaaggaaag atcgatgctg cggcactgaa gaaagtggaa gatgaagcca tcacagagct
     2881 tgtgagcaag atcaaagagc tgggctatca tgtgatcaca gacggtgaat tccgcagagc
     2941 cacctggcat ctggatttca tgtggggatt cgacggcatc ggccataccc ctaccaagac
     3001 gggacttccg ttccacggcg aggcggccat ggtggatgat acctatattg taggcaagat
     3061 cggactgacc ggggagcatc cttttgtaga tcatttccgg tttgtaaaag cattagagga
     3121 tgaaaatacc gtagcaaagc agaccatacc ttctcccgcc cagtttctgg cacagtttac
     3181 catgcccttt aaccgaggct gcacagagaa gcattatccg aatgagcagg aactggtaaa
     3241 tgacatcgtg gcggcctacg gaaaagtgat cgatgatctc tatgcggcag gatgccggaa
     3301 cttacagctg gatgactgca cctggggcat gttcgcagac aagatcggtc ataccctgta
     3361 cggaaccacc agagagggaa ttgtggagtt ccagaaagcc cacaaggata ttaacaataa
     3421 ggtgatcgcc aatgcaccga aggatatgat catcaacacc catgtatgcc gcggtaattt
     3481 ccattccacc tacgcaagcg agggtgccta tgattccgta gcggatatcc tgttcggaga
     3541 agaggatgtg aatgcctatt tcctggagtt tgatgatgag agatccggcg gtttcgcacc
     3601 tttggccaag gtatccgggg agaaaaaggt cgttctggga ctgatcacta ccaagtctcc
     3661 cgtactggag gacaaacagc ttgtcattga cagaatccat gatgcggcaa aatacattcc
     3721 actggaaaga ttatacttaa gcccccagtg cggatttgct tcctgcgaga tcggcaacaa
     3781 gctgaccgag gaagaacagt gggcaaaact ccgacttgtg aaagaggtgg cggaggaagt
     3841 ctggggaaaa taaa
//
LOCUS       DAWDHD010000216         3831 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_1404_length_3831_cov_10.203655, whole genome shotgun sequence.
ACCESSION   DAWDHD010000216 DAWDHD010000000
VERSION     DAWDHD010000216.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3831)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3831)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3831
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1404_length_3831_cov_10.203655"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1101 a          770 c         1045 g          915 t
ORIGIN      
        1 gacaggtagc ggaggaaatc gggaagaaat acgatgtggc cgtgatgccg gtgaactgcg
       61 agcaactgaa aaaggaagat attaatcgtc ttctggaaaa aatcctgtat gaatttcctc
      121 tgacagcagt ggaattttat ttcccgaaat ggatggatat gcttccggtg gatcatgcgg
      181 taaaacagga cctgttaaga cagatccggg aattgatgaa cagctatgac cggattcggg
      241 atgtggcggg acaccctgtg gaattgcagg gagattatat tcaaagctgc aagatggagc
      301 cggtgcagtt gtggaacggt attgttccgg tacaggtagg aatcgacgac aggtattatt
      361 acgaaatgct ttccggaatg ttaggagaga acgtagagga tgaataccaa ctgttgaata
      421 aactaaaaga gttggcagaa atgaaaaagg aatatacgaa ggtgcaggat gcattgtcca
      481 tggtgaagat caagggctac ggcgttgtga ctccggacag agaagacatt gcactggaga
      541 aaccggaatt gatccgccat ggcaacaagt ttggtgtcaa aatcaaggcc agcagcccct
      601 ccatacatat gatccgcgca aatatcgaga cagagatcgc tcccatcgtg ggcacagaac
      661 agcaggctca ggatctgatc cattttatcg atcaggcgga ggcggagcac ggtatctggg
      721 agactaatat cttcggcaag actgtggaac agctggtgga tgacggcatc accacgaaag
      781 ttgcttccat cggagaagaa agccagttga aactccagga taccatgcag aagatcgtca
      841 atgacagcaa cggtggcatg gtttgtatta ttatatgatt ccataaccca cccatagaaa
      901 gtctgctttg cctgcaggca gtctcgcaga ctttccatga ggggagtaaa tacacatgag
      961 cagggagaac gatagtccga aattcgaatg tgggttacaa tttcgagagt gcgaagcacg
     1021 gagaaattgt atgggatgtt agcttttcat tgcttttttc ccgtgcattt tgtataatac
     1081 ctttgttgac tttgctttat ttttctgaaa aacaggaaga atatttgcta aattgcatga
     1141 aaatacggct aaacggagga tatagatgaa tcaggtatat gcaaaattac agaactgtta
     1201 tgagtgtgtg agaaaaaaga cggattttgt acctaaggtg gcgatcgttt tgggatcggg
     1261 cctgggcgat tatgcggaac agatccgagt ggtgacagag atcccttatg cggagatcga
     1321 gggatttcct gtttccaccg taccggggca tgccggtaaa tttatcttcg gttatctcga
     1381 tgagattcct gtggtgtgta tgaagggaag agtacattac tacgaaggtt atgatgtgac
     1441 ggatgtggta ctgcccacca gactgatgaa actgttaggc gctgagattc tgtttctgac
     1501 caatgccgca ggcggtgtga atacttcttt ccatgctggt gacctgatgc tgatcagaga
     1561 tcatatttcc gtgtttgccc ccaatcctct gatcggtgcc aacatggatg aactgggact
     1621 gcgttttccg gatatgagcc atgtgtatga caaggactta cagcagatca tccgtgagac
     1681 tgcgaaggag aacggtatct atttacagga gggtatttat acccagctga ccggcccgtc
     1741 cttcgaatct ccggcagaga tccgtatgct ccggacactt ggctgtgacg cggtaggcat
     1801 gagcacagtg gtggaagcaa ttgctgccaa ccacatggga atgcggatct gtggcatctc
     1861 ctgtatcagt aatctggcgg cgggaatgac agatcagcct ctgaatcatg aggaagtaca
     1921 ggaagctgcg gatatggcag ctcccagatt caagaagctg gtaacggaat ctgtgaagaa
     1981 atttgtatag acacagagaa aaaggagatg cggatgatcc aggagacaga agaaaaggca
     2041 ttggtcagcc aggcattgga agccagaaaa ctggcatatg ctccttattc cggttatacg
     2101 gtgggagcgg cactactgac tgcggatgga cacagatacc ttggcggtaa tattgaaaac
     2161 gcttcttacg gagctacgaa ctgtgcggag cgtaccgcct ttttcaaggc ggtcagcgag
     2221 ggagaacggg aatttacagc cattgccatt gccggaggaa tatccggaga ggcaccggtg
     2281 gaatatgcgt atccatgcgg tatctgcaga caggtgatgc aggaattttg cagggatgat
     2341 tttgtgatct acgtggtaaa aagtgaacag gattatcaac gctatacctt aaaagaatta
     2401 cttccctttg gatttggagg agatgcaatc agatgaatat cagattatat aatgcacgaa
     2461 ttctcaccat ggaacccggc agagaagtct tctatggtga agtatgggta taaaatgaca
     2521 agatcgccta tgtggcagaa cagaaagaac ttgcggagga atgggacaaa ttccagtttc
     2581 ccagaattgc gtgggatatc gaactggatt gcaagggaaa ccttctgatg cccggattta
     2641 aaaatgcaca tacacattcc ggtatgactt tcctgcgctc catggcagat gacctgcctc
     2701 tggatcagtg gctgaacaag caggtatttc ctctggaagc aaaactgacc ggtgaggaca
     2761 tctatgaact gaccagactg gcagtgctgg aatacctgac ctcaggagtg acttccattt
     2821 tcgatatgta cctgacaccg gatactattg ctgaggcctg tctggacacc ggaatgcgtt
     2881 gtgtactggt atccggactg aataatttca gttcttctcc aaaacaggta gaagaggagt
     2941 ttttaaaatg gaataagaag aattccctga tcagttacca gctgggattt catgcggaat
     3001 atacctgttc caaggaactg ctctggaaag tgtcggaaat ggcacatcat taccggacac
     3061 cggtatatgc ccatatctcc gagacggaga aggaagtgga agagtgtaag gctcgctacg
     3121 gaatgacacc gcccatgttc ttagactctc tgggaatgtt tgattttggc ggaggaggtt
     3181 tccactgcgt tcatgtgacg gaacaggata tggatgtctt ccgcagacat cggatgtatg
     3241 tgatcacgaa tcccggctcc aacacgaaac tggccagcgg cattgcaccc attgccgact
     3301 atgtgcggca caagatcccg gtagccattg gaaccgatgg accggccagc aataactgtc
     3361 tggacatgtt ccgggagatg ttcttagtca ccggcttaag caaactgtta gagaaggatg
     3421 cgtccagcat ggatgccgtg gaggtactga agatggcaac ggtgaacggt gccaaggcaa
     3481 tgcgcctgaa ccgcgcagat gttcttgcaa agggaaaata tgcagatctt attatgatcg
     3541 atctgcacca gcccaatatg cagcccattc acaacatccc caagaatctg gtttacagcg
     3601 gtagcaagtc caatgtctgc atgaccatga tcggcggcaa gattttgtat cgtaacggag
     3661 aatttaatgt gggagagaat ccggaacgta tttacacaaa atgcgagaag atcgtaaaga
     3721 gactgttgac tgagtaagaa aaataactca gaagacatag aacggagctc ccgcgtggct
     3781 cccagaatac ttaggaggga aaagatgtta gaaaaaatct tcaaactgaa g
//
LOCUS       DAWDHD010000217         3805 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_1416_length_3805_cov_10.247733, whole genome shotgun sequence.
ACCESSION   DAWDHD010000217 DAWDHD010000000
VERSION     DAWDHD010000217.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3805)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3805)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3805
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1416_length_3805_cov_10.247733"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1149 a          796 c          955 g          905 t
ORIGIN      
        1 cataattatt gagtaggtat cggcacccga aacgacggca aaccttacag caactccgaa
       61 gacaacaacg gcttcgaata gtagcgggaa aacaggtagc atacattcaa aatatgtgtt
      121 ttgtcataaa aatggggagt ggataaaaac cgatgcctat gaaacttgtc ttagacgctt
      181 aatgtctggg ttggggtata atgtgacaaa caatcattct tttcgtatga gcttaaacag
      241 caatgttttt ataggcaaat atgggttacc tgctacagag agagcaagat tgctggggca
      301 ttcagtagag acaaatttaa gatattatag ttttgccgga aaggacagct tggatgatat
      361 ctgtgcaata ctgaatgaag agattgaggt ctcacctagg tctcaccaaa atatcgttac
      421 ttatacaaat gaaaaaagct caagaaccgc atattctcga gctttctaga acaaaatcct
      481 aatgcggaag atgggacttg aacccacacg gtattgctac cacaagatcc ttagtcttgc
      541 tcgtctgcca attccgacac ttccgcatag cgacacaaaa taattactat ctcgcatcac
      601 gcaagaatga taataacatc ttcccacggc aatgtcaaca tgattttgaa atttgtagga
      661 attatttata cacttagaac agtttgggag aaatgtaaag atagaaaatt atgacgatgg
      721 cgtcttagat ttatttctga tataatacat aacagaaaat acactacaga tgattattgg
      781 aaaaatataa acagaagagg gaacaacagt tatggaatca agagtggaac aagcagcaga
      841 gagaaaaatg tgtggttaca actgtgcgca ggcagtggcg tgcacgtact gtgatctggc
      901 gggaattgat gaagagacgg ctaggaatct tactcaggga tttgcggtag gaatcggcgg
      961 cagcatggaa gctacctgcg gagctgtcat cggagcggtc aatgtactgg gactgatcaa
     1021 taaaaatccg cagaagacca tgcaagggtc cagaaggata attcagagct tcaaggagca
     1081 gaacggaacc gtgatctgta aggaattaaa aggcatcaac gatggcattg taaagagaga
     1141 atgcatcgac tgcgtcagag atgcagcggc tttactggaa gcagaattac cggaaagcta
     1201 tggaaagtcg aaaccggtag taccatagag gtgtgacagc atgagtacaa aagaaacttc
     1261 agcggataaa acaaaatacc gcctagcgga agcggcgaaa aagtgtatgg cttcgtccgg
     1321 caccgatgcc atgacagtga cgcagatcgt gcagacctgc ggcgtgacga ggcagacctt
     1381 ttaccggaat tttcaggaca aatatgatct gatcaactgg tatttcgaca agctcttact
     1441 ggaatctttt gcacagatgg gagacggact taccgtatac gaaggactga ccagaaagtt
     1501 tgaattcatt cagaaggaga gagttttttt cgccggagca ttccgctcgg atgatcataa
     1561 ttctctgagg gaacatgatt acgaactgat cctggatttc tacacgcagc tgatcgggcg
     1621 taagaccggt agacagcccg aagagaatat tttattcccg ctgcggctct attgcagagg
     1681 ttctattgat atgaccgtgg aatggatctt cgctccgaag gagagaacgc cggagcagat
     1741 ggcggcgctt ttggtcagtg cacttcctat gccggtgaca gaattgtttc aacagttggg
     1801 cgtattgcgt tagtgaaaaa atcaccaaag ctatttgtga aaaattacta aaaaaaatgt
     1861 acacattgtg aactttctgt aaaaagtgac agtcaagcca aactgtcact ttttttatgt
     1921 ccagattgta gttagaataa aattatcatt aagatttgtt ctcaattcga aaggagatta
     1981 tgtatcatgg caaacagaat tatgctcaac cagacctctt atcatggtgc cggagcaatc
     2041 gaagaaatcg taaatgaagc aaaggctcat gcttttcaga aggcattcgt atgttccgat
     2101 cccgacctga tcaagttcgg tgtgaccgga aaagtaaccg gactgcttga caaggaaggt
     2161 cttgcttatg aaatttattc cgatattaag gctaacccca ccattgacaa tgtgaaaaac
     2221 ggtgtggctg ctttcaaggc atccggcgca gactatatca tcgccatcgg cggcggctcc
     2281 tccatggata cctccaaagc catcggtatc atcattgcca accctgaatt cgaagatgta
     2341 cgtagcctgg aaggcgtagc acctaccaag aagccctgcg tacccatcat cgcagtgccc
     2401 accacagcag gtaccgcagc agaggttacc atcaactacg ttatcactga tgtagagcgc
     2461 aagcgtaagt tcgtatgcgt agatcctcat gatatgccta ttatcgctgt ggtagatccc
     2521 gagatgatgt cttctatgcc caagggactg accgcttcta ccggtatgga tgctctgacc
     2581 cacgctatcg aaggttacac caccaaggcg gcatgggaga tgaccgatat gttccacctg
     2641 aaggcaatcg agatcatttc caagtccctg cgcggcgctg tagccaatac caaggaaggc
     2701 cgtgaaggca tggctctggg tcagtacatt gccggtatgg gcttctccaa tgtaggtctc
     2761 ggtatcgctc attccatggc acataccctg ggtgctgtct atgatacccc tcatggtgtt
     2821 gcctgtgcaa tgatgcttcc tatcgttatg gaatacaatg ctgactgcac cggtgaaaag
     2881 tatcgtgaga ttgcccgtgc tatgggtgtg gaaggtgtag atcagatgga tcaggccgcc
     2941 taccgtaagg ctgcagttga cgcagttcgt aagctgtccg aggatgtagg aattccttcc
     3001 aagctggaag cactgaagga agaggatctt cctttcctgg cagaatccgc tcatgcagat
     3061 gcctgcgcac ccggcaaccc taaggatgca agcatcgagg atctgaccga gctgtttaga
     3121 aagttgatgt aatcattaaa tttgctactg tttttccgaa aaataatatg gtatgctaag
     3181 aaacgagcag gcttgcgaga gcagacctgc tcgtttttaa aagaaaaatg gatgaaatca
     3241 acaggacaaa ggaaacaata atgggcaata caaaacatac caaagagcag atccgtgaaa
     3301 ggatccaaca gaaaaagatc ctctttgacg gagccttcgg gacctattac ggaggaaaat
     3361 atgacacgaa acagctgccg gagctggcga atctggaagc cccggaacgg gtgagggaga
     3421 ttcatacgga gtacctcgag gcaggagcac agattctgcg taccaacacc ttcgcagcca
     3481 acagcttctg catggatagg tccaaagaac agatcgagga gactctgcga agcggcgtac
     3541 gcctggcgag ggaagcggtg gctgcgtgga gagaacgcac cggtgagaca aaagaagtct
     3601 atattgccgg agatatcgga cagattcccg gggatgcgct ggcacagaag gatactttat
     3661 gccgcgaata caaagagatc tgccgtattt ttctggaaga gggagcggac ttttttgtct
     3721 ttgaaacttt ttccgagatg gaagagctcc tgcctgcgat caaaatgatc ggggaacagg
     3781 catttattac ggtacaattc tctgt
//
LOCUS       DAWDHD010000218         3756 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_1441_length_3756_cov_10.066739, whole genome shotgun sequence.
ACCESSION   DAWDHD010000218 DAWDHD010000000
VERSION     DAWDHD010000218.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3756)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3756)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3756
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1441_length_3756_cov_10.066739"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          856 a          971 c          845 g         1084 t
ORIGIN      
        1 gcgacctttt ctgtactata cacctatttt tctcatttgt aaatatagat ttttccttga
       61 attgtcagat aatttgtcaa ttcagttgtt cgtatggctg ttgtctgtga attgtgctaa
      121 ttttcttttt taagccatgc ttttagcaca actctttggg gattttccct atttttcctg
      181 tctccgacca tacactttgc ttgtcagtcc ctgatagatt gcagagaaat gtctgctttc
      241 tgccattgcg gtccggagag gtgccagggt cagtgccatc acagcgcggt aacagaacag
      301 ttctacgggt ttcatgtatt ttgccgtgat ctccttatgc tctgcggcac tgcgtttcag
      361 gttctccttt gtctcggagt agccgccgcc ttcataggaa gatacggtaa gccccagata
      421 ctgcatggga gccttcgccc ggtaatggca ccacagaaaa tgttcataat ctgcgcggat
      481 cttgtatttt ggttcgtagg gcttctgtcg gcacaggtct gcagcataga agcaggactg
      541 atggcaggga atgttgcggt agcagacaaa gccggtgatt cggggcgggg atgatatctt
      601 cgtgtccgtt ttcgcgctgt aggtatctcc gtaatagatg ccttgcagtt tcatagggtg
      661 ctgttccatg aattgtgcgg tgtgtgccag aacttccttg tcatagaaga ggtcaccgca
      721 gtttaagaac agaatgtaat ctccggtcac atgagagacc gcctggttca tagcctcgta
      781 gatactgtga tccggttcct ggtagagatg gatccgcccg tcagtgcgca tggactctat
      841 ggatccgtca cggctgccac cgtccttgac gatgacttcg aagtctgggc aggtctgggc
      901 caggatactg tcaagggtct gattcagttt ggtgccgggg ttcaggcata ctacgataat
      961 gctgaatttc atatgttgct ccttttaaga tataatatct ccgcagtcga gaacagggca
     1021 aggaacgcgg cacgttcgca ctctgtcgca gagcgtagcg gcacgtaagt gaccaaagta
     1081 cggtcactaa gtgccgacgt ctgcttacgg cctgcttatc cttaccctgt tttcggctgc
     1141 tgggttatga agtccagttc aatacagttt atgaatgcgg tcagaactgg taactaatgt
     1201 gttttgttat ctcattaaaa tatatttttc tgtttgtgca aagtgatcaa ggatacaact
     1261 gccactgcca ggaaaatcac gcatacaggc cagctgacga agccggtgac ggcgatggcg
     1321 gtgtaggaca ggcagtaggc gaggacattg gatgccacat gaacgaacac tgccattttg
     1381 aattctccga aatactcata agcgtaggcc atgaggcagc caatgagaaa gccgtagatg
     1441 ccctgcacgt agttcatgtg gtacacaccg aagatcagtg cggacagagc tatagcaagc
     1501 ttcagatcca taaatctgcg caggtaacca taaatcactc cacggaacag taattcctcc
     1561 gcaatcggag agataaagcc gaataccaga atacccacaa taaagtgtgc agaatactgc
     1621 tgtgaaacga cttccgtata ggcttcggat ttattggtca cacccagaag ttcaaacagg
     1681 aggttgaaac cgataacggc accgatggtg gctgccacca gcagggcata ttttgcctta
     1741 ccttcttttt tcagatggct taaatgcata tcctctttgt tcttcgtgat cagaagcttt
     1801 gccgtggacc ataccgccag tgctccgccg atgaagccca gtgccgctat gatactggac
     1861 acatctccgg aatagccgtt atatgcaccg gtctcatccc tcaggatcag cagatctacc
     1921 agtccaccgg aagcaccaga ctgtgccagc tgatacaaca gtgccagcag cgcatactgc
     1981 accgcatttc gcaccaggat aaacatcagg aacgaataca ccatagtcca gattctctgg
     2041 tacatgggat ttttcttttt attgtcggga ttgctgttca ccagttcctc gctgccgcga
     2101 agcagaaatt tttccagttc ttccacggat tgtacgatgt agtccgcttt tgcttccttt
     2161 aactcttcct tgcttccgta gccataagtc acgcccacgc gctccacgcc caacgctctg
     2221 gctccttcca catcgaactt tctgtcaccg atcatgtaga cctgatccgg ctctatggga
     2281 ttctccttga acagctgatg cagggtctcc tgcaccacct gatccttcgt ctctctggtg
     2341 ccgtccagct cactgcctac caccacggca aaatattttt tcagatcaaa atgctccagt
     2401 atgcgttcca catagatctg gggtttgctg gaagccagtg ccagaaaata gcccttggat
     2461 tgcaggttct tcagcatctc cggaataccg gcataaactt cattttcaaa aatacccgta
     2521 ttctgaaaac gctctctgta ttttgccacg gcctcctccg cctgatcagc ggtaaaaccg
     2581 tagaattcca taaagctgtc ccgtaagggc ggtccgataa aaggctccag cttgtccaga
     2641 tccggttcct caataccgaa agagtgcagc gcatactgca cgcaggtcgt aattccaatt
     2701 ttgggatctg tcagtgtacc gtccagatca aacagcagat attttttcat tcttataatt
     2761 cctttttttg tatctgttcc aagccctgta actatcgcaa aatattcatt gaataccggg
     2821 atccgcaaga atattctgta attgttatga ggctctgaat agttaccctt tctttctata
     2881 gcttgtgggg tccgcaaagc attagaagct gcggatcttg tccccgtcat cctcggtgtt
     2941 tttcagtgcc ttgatctcta cgtcatagcc cacattggca ttgacctgca catggaccac
     3001 atcacccact tttttgccaa gcagtgcctt gcccagcgga gattcgttgc tgatcagacc
     3061 ttccagtgag tttccgcgaa cggtggtcac gatcttgtaa tcctcttccg tgccgtcgtc
     3121 gatgaattcc acggtaacag tgttgttgat gcccacctca tccacggcgg agttctcgga
     3181 gatcaccttt gcggtcttga tcattctctc cagataacgg atccggctct cgttctgatt
     3241 cttgtatttt ttcgctgcat aatattcaaa attctcactg agatctccgt gggctctggc
     3301 ttccttcacg tcctccagtg ccttgggacg caccaccagc ttacgatact cgatctcatc
     3361 ttccattttc ttaatgtcag tgggggttaa ttcatcgtac ataatttctc catttctatc
     3421 tgttcaatta cttatatcat ttattgacag tttttctccc ggctcggcac atgctgattt
     3481 tctaccaaca gccgtccttc tttttcttcg gcgaccgacc ttcttttcct tcagcagacg
     3541 cctttctttt cttctgacgg ccgacctttt tccttccggc agccaacctt cttttcttgc
     3601 agaggatgac cggtcgcagt ctctatgctg tcccggtgtg cccagcattc tacaactgca
     3661 ttgcgtagat taccgccaga gacagaatga tctgaataat ggcaaaacgg aatacggtct
     3721 gtgtattgga ccagtcccac accttacgca catgat
//
LOCUS       DAWDHD010000219         3696 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_1464_length_3696_cov_9.686625, whole genome shotgun sequence.
ACCESSION   DAWDHD010000219 DAWDHD010000000
VERSION     DAWDHD010000219.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3696)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3696)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3696
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1464_length_3696_cov_9.686625"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1071 a          754 c         1003 g          868 t
ORIGIN      
        1 agtatttccc gatggatatc agtctctatg attccaccgg caataacaag atcacggata
       61 ccacaggatt gtccatcagc attaccctgc cgattccgga ttctctgatc acctatgcag
      121 gtaacaacaa ggtagcagga gtggtgaaca gtaagttaga caagctgaca ccgaagttct
      181 ccacgatttc cggagtatcc tgcattacct ttacggcgga gcatttctca ccgtatgtga
      241 tctatgtgga tacgaacaac ctgacagccg gaacaatcgc agacagcact cctaagaccg
      301 gagatgccat tcatccgaag tggttcctgt ctctggggct agcatgtctg tccatagtat
      361 tcttcatgaa gaaggataag aagactctgc gcaaggcaag agcctagtga aaataaagga
      421 tgggaaggtt tatgaatcat aaaaagtggg tagttgcatt ggtattgata gctaccgcat
      481 tgatcctgac acatcttcct gtgtcggagg cagatgcggc agcgtctgcc tccgattttc
      541 agattgaagg cagtacactg acaaaataca gaggaagcga tgaacgtgtc acgattcccg
      601 atacagtgga agtcgtgggt gagagtgcct ttgagaacaa tcagaaggta cagtttgttg
      661 tggttcccaa atctgtgaaa cgactggatg cctatgtgtt ctggggctgt aatcatctgg
      721 aagaggttgt acttggcaaa ggtcttacct cagtggatga atatgctttt tcaggatgca
      781 ccggactgaa acagatcacg atccctgaga atgtacagag tattgatgca caggcatttg
      841 ccggatgtac gaaccttacg gatatttata ttccggacac ggtgacaagc atcgcagacg
      901 atgcattctt aaattgtgat aacgtaacga ttcatgcgga cgaaggcagc gtggctgaaa
      961 agtttgcaca gacgctggca gagcagaaaa gtcagagtcc tctggtaacg gcagcgccga
     1021 cggcgacacc ggcggcagac ggaacttcgg attcacaggc aacaacagag cctgccccca
     1081 ctgaaacgcc gacccctgca ccgacaccgg tgcccggaaa tgtgatcggc agtacctttg
     1141 tcgtaggaaa tcatgcgatg gttatgatgc tgcctgagga agaaactgtg caacagggct
     1201 atacggaacc ggtagcagga caggagactg acggtgaacc gcagaatatc acagcggaaa
     1261 cagaagacgg aaaaattccg gaatggaaat attacaggaa ccagtcggta ggagcagtga
     1321 gcataccgga gggaacgacc gaaatcggac gttttgcttt ttccagaagt tctgtacagt
     1381 cgattacttt accggaaggt gtgaccacta ttgattatgc agcattttat cactgtgatg
     1441 atctgaatag tgtgatattg ccggatacgg tgacccgggt ggaagccaag gcatttacgc
     1501 ataccggatg gatggatgat tttgaagaaa acagcatgga tgattacctg atcagcgggg
     1561 acattctggt ggcttacaag ggggaccttc ccgaagtgac cataccggat ggtgttcgtg
     1621 tgatcgctga tgaagtgttc cgcagccata cggaactgaa aaaggtgcat ctgcctgcaa
     1681 gtgtcaccaa tatcggtgac agcgcattcc cggagggaat cgaaattata aatgaataag
     1741 ggtcagattt taccgacacc aggataagaa accggcataa gatagaataa gtcttatgcc
     1801 ggttttgtct tattttacgg ggaaggaaca aaatgcagcg ggtaagggaa caggtacagg
     1861 tgccggaaca attgcatagt ggggcaggac gtgtgaaatc tgtgactgtt gctgtgctgg
     1921 ataccggaat tgcttatcat ccggatctgg taggacggct gcttgctttt tccgactttg
     1981 tagaaggccg ttcctttccg tatgacgata atggacacgg gacacatgtc tgcgggattg
     2041 tatgcggaag cggagaactg tccggaggaa gatttcgcgg aatggcaccg gaggcaaagc
     2101 ttgtggtggg aaaagtgctg gacagacagg gggagggctc ctgtgattcc atgcaggaag
     2161 ctctggaatg ggtactgcgg gtgaaaaaca ggtatggaat ccgtgttctg aatatctctg
     2221 tggggatcgg agacctgaag gaacgttaca aggagcagat gttgcgaaaa tctctggaat
     2281 tgttgtggga tcataatatt ctcgtggtct gcgctgcagg taataccggt ccggaggatg
     2341 gtagtatttc ggagatggcc agtagcagaa aggttctgac ggtggggtgt catgacggaa
     2401 gatattgtaa aaatgatccc ggaaggtgtg agacgtactc cggcaggggc agaagatatg
     2461 atgtgacccg taaaccggat attgtggctc cggggacaag gattctgtcc tgtaatgctt
     2521 tctggcagaa ccggaacggc agaatctaca gaccctatgt agcaaaaagc ggcacatcca
     2581 tggcaacacc cattgtatcc ggggcggcag cccttgctat ggaaaaatat ccgggaatta
     2641 ccaatgaaga atttgttcgt ctgctgtccc ttacagctac cgatctggga gagccgtgga
     2701 acaagcaggg cttcggcatg cttaatgtac ggagattatt agaaaacaag taaaaggata
     2761 aaatcactgg gaaaaataaa aaatatgatt aaaataagtc aataaaataa ataccaaaga
     2821 tattgacaca aatggtatga ttgtatacaa tgatacgaga actgcaaaat gtatttttac
     2881 tgcgcagagt aaaaattata aaatcttgca gatatcgaat gtcagggaag agaggtatac
     2941 caatgagaga aagtgatgca tttcagaatc gtccggtctc catgaatccg aagaacaacc
     3001 ttttggagat tgaagagcat atgtacatac tggatgatgt ggagaagccg aacgtgttcc
     3061 gtaacatgtt cccttattcc gaaatcccaa aaataccttt taatgatcgt atcgtgcctc
     3121 ataacatgcc aaaggatatc tggatcacag atactacctt cagagacgga cagcagtcca
     3181 gagcacctta taccacagag cagatcgtaa agatctatga ctatctgcat gaactgggtg
     3241 gccccaacgg aatgatccgt gccagtgaat ttttcctgta cagcaagaag gacagagacg
     3301 ctgtttataa atgtatggag aggggataca agttccccga agttaccagc tggatccgtg
     3361 ccagcaagga ggactttaag ctggtaaaag agatcggaat gaaggagact ggcattctgg
     3421 tcagctgttc cgattatcat attttcctga aattaaaaat gaccagaaga caggccatgg
     3481 atcactattt aagcgttatc cgtgactgcc tggaggaggg tatcagcccc agatgtcatc
     3541 tggaggatat caccagagcg gatatctacg gttatgtggt tccgttctgc ctggaactga
     3601 tgaaactgat gaaggagtat cagatcccca tcaaggtccg ggcctgcgat accatgggat
     3661 acggtgtcaa ctatcccggt gccgttattc cccgta
//
LOCUS       DAWDHD010000220         3688 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_1467_length_3688_cov_14.845857, whole genome shotgun sequence.
ACCESSION   DAWDHD010000220 DAWDHD010000000
VERSION     DAWDHD010000220.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3688)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3688)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3688
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1467_length_3688_cov_14.845857"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          836 a         1053 c          718 g         1081 t
ORIGIN      
        1 tacttttctt cggaactttg gcgcaaacac aatatggtac ttacaattcc atttactgtg
       61 tgataaacta tttacatcca ctttggatac ctcctttgat tagtgcggtt ggcgaaccaa
      121 cactattata tcgttggagg tttttactgt aagctaaagc gattcttctt ttatttgcac
      181 tgttctaaaa atttcaaagc atccctactc cttctgtgcc gtcaggcatg ggtatgcttc
      241 tataaaaaat agcgatactg tacgaggtgt aatcacctac ctacaatatc gctgctttcc
      301 tgtagattct atctacaata accgttctca caattaccat tccctctgtg aaaccaagca
      361 gaacatattc ctgtatacca caatgttata catggtgttc gacttgttac ggtttaatgg
      421 agtagacggg agtcgaaccc gtgtccaaaa gcctattccc tgtccttcta cgagtgtagt
      481 ttattctttg acattccctc cgcgccccgg gaataaacac cctgagcatt tcagtagctt
      541 catgttacgc ccatatgcgc aaagctttgc atatgtcgtt tctcacatgt ttgaagccgg
      601 gatcctaagg tgtgagtgcc ctagggccga ctgctgccat taggcagcgt atgctaaatt
      661 atcgttagcg tttaatttta gtttggccat ttaacgcatt accatgcgac tcgcttctcc
      721 agctgcaaaa cccctgtcga aacctttact accccttatt caattgtcag accaagtata
      781 cttgatcgca cacacactgt caagcacttg cagtaaagca ttgcaggatc agagcctgtg
      841 gatcacagat tgcggacctt gaattccttc tctgcctctc tgcgctgatc ctttttggca
      901 atggcttctc tcttgtcata gagcttctta ccacgtgcca gcccgatctc tacctttacc
      961 ttaccgtctt taaagtatac ctgcaaaggg actaatgtat accctttttc cttgatcttg
     1021 cccagcagtt tgttgatctc atacttatgc agcagcagtt ttttcacacg aagaggatct
     1081 ttgttgaaaa tattcccctt ctcataagga cttacatgca ttccgtagac gaatacttct
     1141 ccgtcttcaa tgcggacgaa cgcctccttg atactgcact tgcccatacg catggacttt
     1201 acttccgtgc cgtgcaaggc aatgccgcac tcataggtct catcaatgaa aaaatcatga
     1261 tatgcttttt tattgttcgc gatcagcttc tgggactctt ttccttttgc catggctacc
     1321 tcttcaaaac cttgtaacta ttcagaaccc cattgcagaa cactcctgcg gaacttattt
     1381 ttaacggctc tgaatagtta cagtttacta tattttccgt atttgtcaat gaactaacag
     1441 ttcatttgat tcataggttt atttatccac cagccggaaa ttgatggtgc gcataaaacg
     1501 gtcgcagtct tccacttcga tccgtaccgg cattcccagt ttgaagcaac gtccggtggc
     1561 ttctcccacc atctcacagg ctgcctcatt gtagtgataa tagtcgcccg ggatcgtgga
     1621 aatatggatc agcccttcca cggtattggg cagttccaca tacagtcccc agccggtgac
     1681 accggagatc acaccctcat attcctctcc gatgtgctgt tccatatatt ccaccttctt
     1741 cagcttgtcc gtctcccgct ctgcctcatc ggcacggcgc tccatctcgg aagaatgctt
     1801 agccacctcc ggcagaatat cccgatagtg ctccactctc tcttccttca ggcgtccccg
     1861 cagctgttcc ttgataatac gatggatctg cagatccgga taacggcgga tcggcgaagt
     1921 aaaatgacag taatactgac atgccagtcc aaaatgtccg gtacactccg tgctgtattt
     1981 tgcctgtttc atgctgcgca gggtcagtct ggcgatcata ggctcttcgg gagttccctc
     2041 aatcttctgt aacagcttct ggatctcctt gggatggatc tcctccgtgg agaccttgct
     2101 gttcctgcgc cccagcgcct tcatgtaata cccgtaattg ctgatgaacg tagacagctt
     2161 ctggatccgc tccggatccg gtacatcatg gacgcggtag acgaagggca gctcctgcca
     2221 gtaaaaatgc tgtgccactg tctcattcgc tgcgagcatg aaatcctcga tcagcatagt
     2281 cgccacattc cgctcgtaag gcttaatatc cagtggatgt ccttccttat ctaacaggat
     2341 cttgcactcc gggaaatcga aatcaatgga tccccgcttc tgtctcttct tccgcagttt
     2401 gtccgcgagc tcagccattt gtcggaacat aggaagcagt tccctgtact gggaatagtc
     2461 tgttgaggtt tctatgcctt ccccggaggt tccgtccggc atatcggcgt tatgctctac
     2521 acactctggc tcctccagca gctttttcac cacggtataa gacatccgct tattgacccg
     2581 gatcacagat tcgcagatct catagtccac gatctcgccc tcttcatcca ccttcatcag
     2641 gcagcttaat gccagacggt ccacgccctc attcagggaa cagataccgt tactcaatgc
     2701 atggggcagc atagggatca cacggtccac cagataaaca ctggtgcctc gttttaatgc
     2761 ctctctgtcc agtgcggaat tctcctgcac atagttcgtc acgtctgcaa tgtgtactcc
     2821 taagtgataa tatgtcccat caaaggatac actgacagca tcatccagat ccttggcatc
     2881 ctctccgtcg atggtcacca tggtcacatc ccgcagatcc ctgcgtccgg cacaatccgc
     2941 cccggatacc tcctgggata ccctctccac ctggctcatg atcttctcgc cgaattccac
     3001 cggcagatca aagccacgta cgatagacat gatgtctaca ccgggatcat tgatatgtcc
     3061 caggatctcc acgacttttc cctcagggct cttcgtattc gtgccgtatc ctgtgatctc
     3121 caccacgacc ttatgtccag tcatagctcc cttcgaccac tccttcggca cgaaaatatc
     3181 ctgtgctatc ttcgtgttat ccggaataac aaagccgaaa ttactcttgg actgctcata
     3241 ggttcccacc acctgagtca ttccccgggc aatgatacgg atgacctgcg cttcctggcg
     3301 tttgccggtc ttcgcaggca gaagtgccac ctccacggta tccttatgga atgctccccc
     3361 ctgcttgtcc tccggaatga acagatcttc gtcccttcct tccacttcca caaatccaaa
     3421 gcctttggca ttgctgataa aggttcccgt cagaacctta ccgttggact tcatatattt
     3481 tcccttaacg gtcagggtaa gctttccttc cgctaaaagt tcccgcagaa tctcacgaaa
     3541 ttcctctctg tcttcctttg acacctgcaa gaacattgcc agttccttct ccttcatggg
     3601 gacatagaaa tcgtccttca ccagatcaca gattactttt tttctcttct ctctgatttc
     3661 gttattactc atgtaacctc tctcaata
//
LOCUS       DAWDHD010000221         3643 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_1487_length_3643_cov_10.417503, whole genome shotgun sequence.
ACCESSION   DAWDHD010000221 DAWDHD010000000
VERSION     DAWDHD010000221.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3643)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3643)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3643
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1487_length_3643_cov_10.417503"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          877 a         1000 c          729 g         1037 t
ORIGIN      
        1 cagaccatac cgtcacctca tgtccgttct tatgtaatac cactgacagg ccgatgcccc
       61 agcttccacc acccaagata catacttttg ccataattat ttgtcctccc ctgagatttc
      121 tgtctgcgct tcggctgatt tcttattctt gaacagataa gtcttcctct cgttcccgtg
      181 gattagacgc acgatattct gtctgtgttt ccaatatgcc atgactgtta aaaatgcggt
      241 gatggcgtac atttcatata gctgtgactg cggcatggtg ccgaacagcc ccgtctgtcc
      301 gcagaccacc atctggatca taaatcctgc atataccaat aatgatccca gagacacata
      361 atgggtggtc aggaaggttc cgaagaacaa taccacgccc atcacgataa aataaggatg
      421 gaacgacagg atcagtcctg ctgtggcagc gattcctttt cctcccttga atcccatgta
      481 aaacgggaag ttatgtccga tgatggctcc tgcaccggta tacaggcaca gcagataaat
      541 attctccgga tgtccggtag tgaacaatgc cttggtgatg catacggcaa cgatacactt
      601 taagcagtct cccgccagta cgatcagtcc tgccttggtt cccagcactc tcagagcatt
      661 ggtggtaccg gcattgccac ttccgtactt acggatatcg attccctttg cctttccgta
      721 aaagtatgcg gtctgaaaca ggccgaacac ataaccgatg agtaaacaga taacacgttc
      781 cattattgat ctttttcctt tcgttctctg ataataaacc ggaggggggt tcccttgaag
      841 ccaaaggtct cccggatctg attctcaata tatctggtat aggagaaatg catcagctcc
      901 ttatcattga caaagatcac aaaggtaggg ggctttaccg ctacctgggt aatgtaatac
      961 aatctcagtc tcttaccctt gtcggaaggt ggctgctgca tggccacggc ctctgccatg
     1021 atctcattca gcacaccggt agccacacgc atggcgtgat tctcactgac gatgtcgatc
     1081 gtctcgaaca gcttgggcag tctctgtcct gtcttcgcag agacgaacag aagctctgca
     1141 tagggcatga aggataaggt attacgcacc ttttcggtaa atttataaat ggtcttgtca
     1201 tccttttcga tagcatccca tttgttcacc acgatgatcg tagcctttcc tctctcatgg
     1261 gcgattccgg cgatcttcgc atcctgctcc gtaacacctt cctctgcatc gatcatcagt
     1321 accaccacct cggcacgctc tacggcactg acggtacgga cgatcatata acgctccagt
     1381 tcttccttga ttttgttctt ccggcgaaga ccggcagtat caatgaaaac atattctttg
     1441 ccgttataag tgatctcggt atccaccgca tctctggtgg tacccgcgat atcagaaaca
     1501 atcagacggt tctcacccag cagtttgttg atcagggaag acttgccgac attgggcttt
     1561 ccgacgatgg ccacacgggt gcggtcatcc tcttcttcct ctgcctgttc cttatcaaaa
     1621 tattccgtta cggcctccag catgtcaccg attcccagac gattcaccgc agagatcgga
     1681 tgaggatcgc caatacccag attgtagaat tcgtagacat ccgccatata cttctggaag
     1741 ctgtccacct tattgaccac cagtaccacg ggcttgtggg aacggcgcag catgtctgcc
     1801 accttggaat ccgcatccac cagtccctgc tttacatcca ccaggaaaat gatcacgtct
     1861 gcagtctcca tagcgatctc tgcctgttcc cgcatctggg acaggatcac gtccttgctg
     1921 tccggctcga tacctccggt gtcgatcagg gtaaatgtca tatccagcca gtggatatcc
     1981 gcatagatac ggtctctggt aataccgggt gtatctttca caatagatat attttcgcca
     2041 gccagtgcat tgaacagggt ggacttaccg acattcgggc gtcccaccac cgcaacaata
     2101 ggcttggttt ttctctttgc cataatttcc tccgtttctt atttaccatc ttaaaatcta
     2161 ttcagatgca tcttctccaa gcagggcatc cacaagatca tatccgcttg attttacaat
     2221 attcacaggt acttgtaaag ccccctccac gtctgccagc gtataatcat ccaggaacag
     2281 atcatctccg cttctgagca cattccgggg aagatacaac gcttctccca agttcttatc
     2341 ctttaactgc tttacgatat cctgtcccgt cagcagtccg gataccgtga tcagttctcc
     2401 gaaaaattca ttgatgatct catagacatg gatctgcaga ccgggatatt cctccatcag
     2461 ttcttccgac atcttcttta aataaggaaa cgccagtctt cccgtagcca gagaaatctc
     2521 tcttttgcct tcccaggatg gctttttccc agcttccttt tctgcggtac gtctcttcag
     2581 tccgtcgtgg aattcatcca ataacaggcg gatcattccg acaccattct ccagctgcag
     2641 atatccgtcg tagcgttcct cttccggcac ctctctgccc gccagaatat accattcatc
     2701 gctggcctgg ataaagtgtg tcccatattt ttccatgcag atcttctggt atttctcgat
     2761 cagatcaatg atctcaccgg catcctgcgc attaaacggc tctaacggat ataatccctc
     2821 ccggtacctg gataatccta ccggcaccac cgagacactt tccacatttg gcagatattc
     2881 catcagcttc cggatgctgt attccagttc cttgccatcg tttacgccct tgcagagcac
     2941 gatttgtcca ttcatacgga ttccggcggc attgaggatg tccacctttt tcagggcatc
     3001 ccccgcaaaa cggttgttca gcattttgca gcgcagttcc ggattcatgg tctggaagga
     3061 gatattgatc ggagataaat ggtatctgca gatccggtca atatcatgat ccgacatatt
     3121 ggtcagcgtc acataatttc cctgcaaaaa ggataatcgg gaatcatcat ccttaaaata
     3181 aagagtatca cgcatgccct tcggcatctg atcgttaaag cagaaagtgc atttgttatg
     3241 gcaatgacgg tattcgtcca tcagaccgtt ctcaaattcg atgcccatat cctcatcaaa
     3301 ttccttgtcg atctctaaga gccactgctc tccgtccggc ttctctacca gcatctcaat
     3361 ataagtatcc tgtgtcagaa actgataatc aaaaatgtct tcgatctctg tattatctat
     3421 ggccagtact ttatctccgg gagcgatctc cagttcctcc gcaatgcttc ccggctttac
     3481 tgtttttaca atatgttctt ttttcttcat tctaattcac ctaaccatct ttttcctatc
     3541 cattgtacaa gaaattcctt gacgtgtcac tagcataatg ttatacatta aatgttataa
     3601 tcagaagtgt tcctcaaagt tacgcataga agatgcgcta ttg
//
LOCUS       DAWDHD010000222         3539 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_1558_length_3539_cov_10.123134, whole genome shotgun sequence.
ACCESSION   DAWDHD010000222 DAWDHD010000000
VERSION     DAWDHD010000222.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3539)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3539)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3539
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1558_length_3539_cov_10.123134"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2010..2109
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1005 a          686 c          936 g          812 t
ORIGIN      
        1 ttatgggaga tgaccacctg gggatatcga atctatgcca tcgctttcat gggccgcggc
       61 atgatcgtgt ggggttcctc cttcttcaca gccctgaaca acggagccgt atccgcagca
      121 atctctttcc tgcgtacctt cgtgttccag atcgtcatcg tgctgatcct gccgaaactg
      181 atcggcatta ccggtgtatg gctgtccatc gtactggcag agttcctggc actgtttgtg
      241 acaataggat tcctgatcgc caagagaaaa aaatatcatt atgcataact attcagagcc
      301 atgcaaactg cgttctgcga atgcttcgga ttgtttcctt gacagaaaaa tgacaaatac
      361 ttatactgaa agtgcacttt gttctctata aaagcgcaga aacgaggaaa tgaaaatggg
      421 tgttttatcg aacttagaac cgaagaaggt attttactat tttgaagaga tcacgaagat
      481 ccctcacggc tccggcaatg tggagcagat cagcgatttc ctggtggact ttgcgaaagc
      541 acataagctt ttctatatac aggatgctat gaaaaatgtc attatggtca aggaggcaac
      601 tcccggatac gagaatgaac cggtggtgat cctgcaggga catatggaca tggtagcggt
      661 gcagacaccg gattgcacca tggatatgaa gacggaagga ctgaaggtag gaatcgacgg
      721 agacagcgtc tatgcggaga ataccagcct cggcggcgat gatggtatcg ctgtggctta
      781 tgcactggca ttattggatt ccgaggacat caggcatccc cgtttggaag tcatcattac
      841 agtggatgag gaagtcggta tggacggagc cagagagatc gacctgtcta tgttacaggg
      901 acatcgcatg atcaatctgg attccgagga tgaaggcatt ttcctgacca gctgtgccgg
      961 tggtgccaga gtgaactgcc gtttcccgat gaaaaaacag gagcttacgg gcgtgcgcta
     1021 tgaggtggaa gtctccggcc tcttaggagg acattccggc ggagagatac ataaggaaag
     1081 aggcaattcc aattgtatcc tgggaagact tctgtggaag ctgtctgaga aaatgcctgt
     1141 aggactggta tctgcggacg gaggactggc agacaatgcc atccccagac agacgaaagc
     1201 agtagtcgta gtagaggaga aagatgcgga aagcttagag cagcaggtag cagctttggc
     1261 agcagaactg ggtgacgaac ttgccaccaa ggatccggac gtaaaggtaa ccgcaaaaag
     1321 attagcagac actgcagaga caaccacctg tgctacgccg gaagctacaa aacgtgtggc
     1381 agcctttttg caggcgactc ccaacggcgt acaggcgatg agcgcagata tgccggggct
     1441 ggtacagact tctctgaatc tgggtatcct gaaagcagat gcggaagttt tgcgggtaga
     1501 gtattctgtc agaagcagcg tggaaagtga gaaagataac ctgatcgcaa agctttctgc
     1561 actggtggaa ctgacaggcg gcgattacgt ggtgaccgga gattatcccg gctggaagta
     1621 ccgcgtccat tctccactgc gggaaaaaat ggtgaagctg tatgcggaaa tgtacggcac
     1681 tgagcctaag gtggaggcta tccatgcagg actggagtgc ggactgttag gcagcaagat
     1741 cacagacctt gattgtgtat ccatgggacc ggatatgaag gacattcata cgaccgagga
     1801 gaccttaagc atatcttcca cgaagagagt gtgggaatat ctggtaaggg ttctggagac
     1861 gaaggactga ctgaatttga gtaacaggaa gtccagagaa gcaaataaga tagaaacgat
     1921 cgaaaaatgt aaagagttag caaattgaaa aagctaaaat ataaataacg ttggaggaaa
     1981 tacactgtca cggcggtgtc aataaaaaan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnng gacatatcaa tgtccatgag accggtgcgg tggaagcctg cggacataag
     2161 gtactgggac tagaaagccc cgacggtaag atcacggcag cacaggtagc agagacttac
     2221 gaagcccata ttcacaacga cagctttgaa cacatggtac agccgaaaat ggtttatatt
     2281 tcaaatccca cagaggttgg aaccatttac agcaaagcgg aactgaccgc tctgtccgag
     2341 acctgtcaca aatacggact gtatctgttc ttagatggcg ccagactggg ctacggactg
     2401 gcagcgccgg acaacgatct gacattaccg gagattgcgg cgctttgtga tgtcttttac
     2461 atcggtggaa ccaaggtcgg cgcattgttc ggagaagcag tagtgatcaa aaatccggag
     2521 cttgcacagg atttccggta tcttatcaag cagaatggcg gcatgcttgc caagggaaga
     2581 ttgctgggac tgcagtttga tgcgcttttc acagatggtc tctatcagga gatctccgcc
     2641 catgccattg ccatggcgga gaagctgcgg gaagcattta cggcgaaggg ctacaactat
     2701 ctggcaccca accggacgaa ccagatcttc gtcattgtac cggatgcaca cctggcgaag
     2761 atttcagaac agttcgagta cagctatgac cagcggatcg atgccacaca cagctgcgtg
     2821 cgattctgta ccggctgggc cacgagggaa gaaaatgtgg atgcactgat ccggtgcgtg
     2881 gaaaaattat aatatgtttg gaagattccg caagccggga aaggtttgcg gaattttaaa
     2941 aatcagtaat caatgaggta tagaacagag gagaaaacac atggataaag aaatgaccaa
     3001 tgccattcag ttaagcatca tttcagtggt tattgggcat tgtttgttag tttaacgatt
     3061 ataggaattg taaaaatact gatggtctat ttcaattttg aacaaggtaa aaagataatt
     3121 accgctttgt ctgtaggact tagtataatt gctgttttgt ttttggctat ggcaagagag
     3181 ccgtatgcga taaccgtaat gttcttatta ttgtttatta aaggcggtct gctttataaa
     3241 caagctaagg caggtcaatg agatatccaa tgaggtcttc tgttaatcgg tcaatgaagg
     3301 tggcatatga aggataaatc attgggaaat ggtttatgga aaggggacgg gaaatgacag
     3361 aaaacagtta taatacaccc tgtggctgca ttcattattt tgtaaatata atagataaac
     3421 agaaaattac gttagtgttt ttaccgggac ttacggcaga ccataggctt tttgaaaagc
     3481 agacagaata ttttgagaag agacagaatg tgtttgtatg ggatgcacca tcacatgca
//
LOCUS       DAWDHD010000223         3537 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_1562_length_3537_cov_6.939115, whole genome shotgun sequence.
ACCESSION   DAWDHD010000223 DAWDHD010000000
VERSION     DAWDHD010000223.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3537)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3537)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3537
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1562_length_3537_cov_6.939115"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          925 a          812 c          976 g          824 t
ORIGIN      
        1 aagttagcgt taaatgatga tattctgatg aagatcgaaa aaccggcccg ctacatcggc
       61 aacgaagtca atgccgtagt gaaggacagg gacagcattg atgtccgttt ctgtatgtgc
      121 ttcccggatg tctacgagat cggtatgtcc cacctgggaa tccagatcct gtatggcatg
      181 ctcaattcct gggatgatgt ctggtgtgag cgtgtgtatt ctccctgggt ggatctggat
      241 gaaattatgc gtaaggagaa cattccgctg tttgccctgg agagtcagga tccgataaaa
      301 gacttcgact ttttgggaat caccattcag tacgaaatgt gttatacgaa tattctgcag
      361 gttctggatc tggcggggat cccgttgctt gccaccgaac gtggagagga ctgtcccatc
      421 gttatcggcg gcggtccctg cacctataat cctgagccca ttgccgattt ctttgatatt
      481 ttctatatcg gagaaggcga gacccagtac cggccgctga tcgatctcta taagaaatgc
      541 cggacggaga agacaggcag agaagaattc ttacgccggg tggcaaagct gccgggaatg
      601 tatgtgcccc ggttctatga gaccacttac catgaaaatg gcaccattgc ttcctttcga
      661 cctacggaag agggaattcc cgcaaggatc cgcaaagagc tggtaacgga tatgaccgac
      721 agcttttatc ctatgaagcc ggtagttcct tatatcaaag tcacacagga cagagtcgtt
      781 ctggagattc agagaggctg tatccgtggc tgtcgtttct gtcaggcagg acagctgtat
      841 cgtcccgtca gagaaaggga tgtggaagtt ctgaaaaagt acgccatgga aatgttgaga
      901 aacaccggtc atgaggagat ttccttaagt tccttaagtt ccagcgatta cagcaagctg
      961 ccggagctga ttgactttct gatcgaagag tgtgataaga gacatatcaa tatctctctg
     1021 ccgtctttac gtatcgatgc cttttctctg gatgtcatga gcaaagtaca ggatgtgaag
     1081 aaatccagtc tgacctttgc tccggaagca ggcagccaga gactgcggga tgtcatcaat
     1141 aaaggactca ctgaagaagt gatcttacag ggttccgccc ttgccttcga aggcggctgg
     1201 accagagtca aactgtactt catgctggga catcccacgg aaaccgaaga ggatatccgg
     1261 ggaatcgctg agctttccaa caaaattgcg gccactttct tcgatacggt gccgaaggag
     1321 aaacgtgtca acggcagagt gcagattgtt accagtacct ccttcttcgt gccgaagccc
     1381 tttacgccat tccagtgggc gccccagtgt accaaggaag agttcgtgga aaaggcatat
     1441 ctgaccagaa aggccatttc cgagcagctg aaccagaaga gcatcaaata caactggcac
     1501 gaagcggatg tcagcgttct ggagggtgtt ctggccagag gtgacagaaa attgtcccag
     1561 gtgcttttat atgtctataa caagggctgc ttctacgatg cctggagtga gtatttccac
     1621 aacgatgtct ggatggaagc ttttgaagcc tgtggtctgg acccggattt ctattcccac
     1681 agagaacggc ctctggacga gatcctgccc tgggatttcc tggactgcgg tgtatccaga
     1741 gcctttctgg agagagaatg gcagaaagcg aaaaacgaga cgatctcacc gaactgcaaa
     1801 caggcatgtc agggctgcgg tgccgccaga ttcggctgcg gtatctgtgt ggagcccaga
     1861 ggatgattgc cacaacagac atcggaagta tagattacgg agttaagaag aaacattatg
     1921 aaattaagaa tcaagtttag aaaatatggt cccattcgtt ttatcggaca tctggatgtg
     1981 atgcgctttt tccagaaagc caaccgcaga gcggagctgg atgttgctta taccgggggc
     2041 ttcagcccgc atcagatcat gtcctttgct gcacctttgg gtgtgggact gaccagcaac
     2101 ggagaatata tggacctgga agtgcattcc ctgacctcct gtgaggatgt aaagcagcgg
     2161 ttgaatgccg cttccgtacc gggaatcgag atcacttcgg tgaaaattct gccggacaag
     2221 gccggcaatg ccatggccag cgttgctgcc gccggatata cggtagcttt ccgggagggc
     2281 agagggcctc attttgatct gggctccgcg gtggatcgtt ttcttgcaaa agatgagatc
     2341 ctcattacca aagagacaaa aaagggaagc cgggagatca atttaaaaga aggtatctat
     2401 gagatgaagg tggtggagtc cgaaaagctt tatctgctgg tggatgcctc cagtgccgga
     2461 aatatcaaac ccattcaggt cattgaagca ttattttccg aaaatggtga gaccctgcag
     2521 gaaaatgccc ttctggtcaa cagagaagag acctatctga gaaacgaaac agacgccctt
     2581 ctccccctgg atgccatagg tttcgacagt gaggaagctt acagggcagc ttccggtgac
     2641 acggaatgag ccgcagtctc accggcggac gaccggccag agaaaaagaa gtaaatcaga
     2701 tccgtaaatc cacggattgc acggagggaa aactgatctt cacctgtctg cgggaacgca
     2761 gggcagctct gcttgtcaat gcccggggcg tggctgctat acgcgtactg cggtcagatg
     2821 ccagtaagat cggtggcatc tatctgggga aaatacagaa tgtggcaaaa aatattgatg
     2881 cctgcttcgt ggagatcctg cccggagaat tgtgctttct ccccctgcgg gaagcaggtg
     2941 cggcgtatct gaccaatcgt aaggcagacg gtaccttgaa agctggagac gagctggtag
     3001 tcatggtcac cagagatgcc cagaagacaa agcgtgcctc cgccactgcg gatcctgccc
     3061 ggatgaagca gctgctttgt aaaaacggga gcacccctga gaacgcttct gaagcccttc
     3121 agagcctttt ggagcaggta gaccataaag tgtacttcac ctgccttttg aagccttctg
     3181 aggccgttta cgaggttctg gagcagatgg cggatccttc ggaatattct gagatcctta
     3241 ccgatgatcc tcagatctat cgtcagctgt tggaaggaga tcaccctttg ttaaaacaaa
     3301 agtccattcg gttctacgat gatccaacga tctctttacg cctgctgtat tctctggagc
     3361 gcggcatgga ggaagcactg gatacgagag tctggttaaa atgcggaggg tatctcgtca
     3421 tcgagcctac ggaggccatg acggtgatcg atgtcaattc cggtaaaaac gaagcgaaaa
     3481 aagaagggta ggacacctat tatcaggtaa agatggaggc tgccgaagaa gaagcaa
//
LOCUS       DAWDHD010000224         3524 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_1571_length_3524_cov_9.422312, whole genome shotgun sequence.
ACCESSION   DAWDHD010000224 DAWDHD010000000
VERSION     DAWDHD010000224.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3524)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3524)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3524
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1571_length_3524_cov_9.422312"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          923 a          928 c          755 g          918 t
ORIGIN      
        1 gagcacttca ctcgtaatga aggggtcgtg ggttcgagtc ccattttcag cttatatgta
       61 ttaaaaaaga gaaagctttt cgctttctct ttttttgtag taactcttca gagccatgca
      121 aagctacgtt ctgcaaatgt ctgcttatct tgcacctttg tcgtagatca ctcccagtgc
      181 tctcggtgca ccgttctgtt tggagaagaa taccagaagc agcaaagtca gtacataagg
      241 caggatcatg accagatcct gataactgct gggcatctgc agtacctgta ccagacggta
      301 tccaccggat ctggcaaatc cgaacaacag acacgctccc agagtcggca ggatacgcca
      361 gttgccgaag atcagtgccg caatagccag ataaccgtaa cctacataaa tactggagga
      421 aaagtttgcg gaaatggaat acgcgaaaca gatacctccc agtcccgaca acgcaccaca
      481 gatcataatg gctgccagac ggatctgtcc cacctgtctt ccggccgcat ccactgcatg
      541 gggattgtca ccgcaggcac gcagatgcat gccgaagcgg gtcttataca tcacaaacca
      601 tgccacgaaa gccactacca caatcacaag ttcaaaagga tacagattgg taaataccgc
      661 tcccagtacc ggaatcttag agattcccgg gatggtaatt ctggaagaaa cagtcagtac
      721 gaacttatcg gacgtagcac caaataccac tctgttgaac agcttcgtta aatatgcggt
      781 cagcgccatg gccagaatat tgactaccac accactgata acctgattgg ccttgaactt
      841 cagacataac atggcatgga tcagtgcaaa cagacttccg cccacagccg ccataaccat
      901 ggcaatgtag aaaggcgccg gagagtctcc ggcaaagttt cccgcaatgg caaccgccac
      961 gaatgcaccg ataaaggcgc caaaaccctg taagccttct accgccagca tggtcacacc
     1021 ggatttctca cagtagatgc caccgatggc cataataaat aaaggcagtg caaaggataa
     1081 accgtcaatg atcacaatat ccatttattt gtcctccttt gtcttgttgg ttttctctct
     1141 tctgtttcgc tccattttcc acttgatgta agcattacat gcgctgaaca aaaggatgat
     1201 accggtgata acggatgcaa tctccgcatc cacgccggcc gcagatttca tataagtact
     1261 tcccttgctg atcagggtga tcagcatagt agaaaataca ataccgaagg gattgttgtt
     1321 accaagcagg gataccgcaa tggcatcaaa tccggtactg atcaatacct ttggctgaat
     1381 ggatcccaca tagcccagat aataagtcac accggcaagt ccggccagtg caccggaaat
     1441 cgtcatggac agtaccatgc ttctgcctac cttgatacct gcatattttg cagcactgcg
     1501 ggaatagccc acagccttca gttcataacc aagctttgtc ttctctaaca ggaaccatac
     1561 agccaccacc gtaagcagag ccagaataat tcccagcgga atatccattt tcagattgcc
     1621 caccatggta tccatcagtg tcagtctgga agctgcagag atttcctttg actgtctgga
     1681 gaccggatcc acgaaaaagg tattgatgaa gaaactgatc acatactgga acgtatagtt
     1741 taacataata gaggaaacga cctcattgat attaaaccgg tatttcagga agccgatcag
     1801 tgcccccacc agaccaccga cgatcaggcc gatgatcact accagcggct ttgccagcac
     1861 ggcgttcaga ctgctgtagc ccacaacgat gcttgcggta aatccggctg ccagcatctg
     1921 tccggagaca ccgatattaa acagtccggc gcggagtgct accgccacag ccagtgccgc
     1981 gaagatcatg ggggtaaaat agttcacata actcataaag tccgtcagca tactcttgta
     2041 gctggcgtag gaggacttgg gtgccagtcc gcctccctgt agaagtgcgt aataagcctt
     2101 aaagggattg gaaccggtca cagcgatgat cacaccacct gccacaagac tacaaaggat
     2161 cgcaaggaga ggaatcttta ccgcattcca taattttgta aattttttca tggtcttatg
     2221 cctccttctc cttgtttttt acgcccatca tgaactctcc cacttcattg gtggtcagtt
     2281 cactggcttt ggcgatcttc tgtatgcttc cgttataaat aactgcgatg gtatctgcac
     2341 aattcatgat ctcatccaat tccagggaga tcagaaggat tgccttgccc ttgtctctct
     2401 ctgcaatgat ctgcttgtgg atattctcta tggcaccgat atccagtcca cgggtgggct
     2461 gcacaaagat catcagggag gaatcctgtt ccacttctct ggcaataatg gctttctgct
     2521 gattaccacc actcatggaa cgcaccactg tcttgctgcc ctggccgctt cggatatcgt
     2581 atttttctat cagtttttca ctgttgtctt caaaaatctt ctgatccagt actcccttat
     2641 gacagaacgg ctctttgtaa taattacgaa gcgccagatt ctccgaaagg gtaaaatcca
     2701 tgaccagtcc gtaattctgt ctatcctcgg gaatatagga aattccttcg gtcgttcgtt
     2761 ctctgataga caggttcgta atgtctctgc cgtttaacag gatcttgccg gaacgcaccg
     2821 gtaccagtcc cgcaatagca tctgcgattt ctacctgtcc gttgcctgcc acacctgcga
     2881 tcgcaacgat ctcaccggaa tgtacctcca gagatacatc ctttaccacc tggaaatcct
     2941 gtttattgta aacattcagt ttctcgatac tgagtatggt cttacccggt tttgcaggct
     3001 ctttttctgt ctcaaaattc acttcacggc ctaccatttt gttggccatg actttggtgg
     3061 acgtggtagc cacatccatg acatccatca gacgtccgtg gttcaggatg gcacatctgt
     3121 ccgcgatccg tttgatttct tccagtttat gtgtgatcag gatgatagtc ttgccgtcat
     3181 cccgcagaga tttgataata tccaacagga aatcgatctc ctgcggtgtc agaaccgctg
     3241 tgggttcatc aaaaatgagg atctccgctt ctctgtaaag catctttaag atctctactc
     3301 tctgctgtgt agagacatta atgtcgctga tcacatcggt gggggtcact gccagtccgt
     3361 atttttccga aaggttccgg atatcctgat tggccttctt catatccact accggaatac
     3421 atcccagcac cttcttcacc ggctccatac ccattacgat attctcagct atggtgtagt
     3481 tggataccag tttgaaatgc tggtgtacca taccgatgtt taat
//
LOCUS       DAWDHD010000225         3369 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_1690_length_3369_cov_10.668980, whole genome shotgun sequence.
ACCESSION   DAWDHD010000225 DAWDHD010000000
VERSION     DAWDHD010000225.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3369)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3369)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3369
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1690_length_3369_cov_10.668980"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          773 a          678 c          997 g          921 t
ORIGIN      
        1 cgcttttgct ttacataata tgttacaaag taatataatg ataccagggt caaaaggtct
       61 aagcccacat gagtttgctc ataagtgggt gaaagacctt gggtagaatt gtggtagtgc
      121 gtagcacgaa gcaatggcag gaaggaaacg ggaatcgata tgcagaaaaa agaacgtatg
      181 gcaaatctgg aagtactgcg ctgtgtggca atgatgatgg tcgtggtgct gcactatctg
      241 ggcaagggag gactgctgcc ggatctgacg gcgcctttat cagtgcagga tatggcagcc
      301 tggctgttgg aagcattctg tatcgtagct gtgaatgtgt atatgatgat cagcggatac
      361 tttttatgcg aaagctcttt taaactgagc agactgctca cgctgtgggt acagctgtgg
      421 ctgtattctg tgggaatcgg tgtgctggcg gcggtgactg ggatcgttcc cgcggcggaa
      481 gtcagcactc actattatct gaccttgttg ttcccggtga ctatgggaca ttactggttc
      541 ctgacagcgt atcttttctt atatatattg ctcccctttg tgggaatggg actgcggcgg
      601 atgacgaagc agcagtttca ggtggcatta gtactgctgt ttgtcacctt ctgcctgtta
      661 aaatctgtgc tgcccttccg gctggaagaa gacggtaaag gctatgattg tctctggtat
      721 ctgtgtgtat tctgctcggc ggcttacctg cgccgatttg gaatcccgtt tttacagaaa
      781 aaatcccggg cattattgtt gtatctgatc ggtatcttcg gaacctttgg agaagccatg
      841 cttttgcacc tgttttattt gaaaacaggc agtctggagc tgatcctgaa gattccgtat
      901 gagtataacc atattttccc gtttctggct tcactggggc tgttctgtct gtttttggac
      961 agcagcatac aggggaagat cggcagcgtg gcggtgaagc tcgcacctta cacgctggga
     1021 gtgtatctgc tgcatgaaaa tctgggtgtg cgctatgcct ggcagaagtg gcttggtgtg
     1081 gagcaaatcg acggtgtact gccgttgctg ttgtggacgg tacttgttgt tgtggtagta
     1141 tttgtactgg gaattctggt ggattttgtg cggaaagcta tctgcgacgg actgcataag
     1201 atattcctgc atatccgtcc ctatcgcagt ctgacagaga aaatacggga cgtagatact
     1261 atgttcaaaa gagaggtaat ggagtgacag agataataaa aaataaaaaa gagggtattg
     1321 acagtcagag acgtctctgg gaatttgtat tcgtggcaat ccttgtgctg tatcctctgc
     1381 ggcatattgc ctggggactg gatctgtggg ataccggtta cggctatgcg aactttgaat
     1441 atatggggac gcagcatatg gattccatgt ggctgttttc cacctatctg accacagcca
     1501 tcggacattt tttcagtttg ctgcccgggg ccgggacttt gatcggcatg aacttttata
     1561 ccggactgag tatcagtctg ctggcggtgc ttgggtatta tttctgcacg aaagtactga
     1621 agatccctgc acttctggta ttcctgggag aatttactgc ggtgagcttc tgctggtgcc
     1681 ccacgggctc tttttacaat tatgtgacct acgtgttcta tctggtatcg gtggtctgtc
     1741 tgtatctggg ccttgcccgg gggaaaaagg ggtggctgtt tgccgccgga gtagcgctgg
     1801 gctgtaatgt gctgtcccga ttctccaatc tgccggaggc ggccatgatc gtgggcgtgt
     1861 gggcttacgg aatcatctgc tggctggaag cgcgcaaaga gattggaaag agtctcggtc
     1921 aggctgggga gacgacagaa actgcggaaa aaataaaagc gcggaaaaag gcagcggggg
     1981 tgaaactccg taagaaatta ttacaggata ccggcatgtg tctggcgggg tatctgacgg
     2041 cactgctggt attattcggt tatatccaga tccgctacgg catggatgaa tatgtgaagg
     2101 gcattctgcg actgttttcc atgacggagg tggcaacgga ctatacggct gtatccatga
     2161 tcatgggcat gttcgactgg tattttcaga atctgtactg ggagatccgg atgtgcgtgt
     2221 tccttgtagt ggggattgtc gctgtgggag tattggaact catcggctcc tacgtgagaa
     2281 aagatacagt aacaaaggtt ctacggatcc tggaatgggc gggaagtatc gcactggcgg
     2341 ctgtgatggt gttctggctg taccgccagg gcttctgtgc cagagaatat acgaattacg
     2401 gtgccattat ctggcccgga gtgacgtttt tgacattgac tctgctggtg accctgtggc
     2461 ggatctttac gccctccgcg ccgaaggaag aaaagctgat cagcggactg atcttcctga
     2521 tcgtatggat cacttccctg ggcagtaaca acaagctata ccccagcatg aacaatctgt
     2581 ttctggcatt gccctatatg tactggcagt tctacaggtt ctgcaaatat gttggcagct
     2641 ttcggtggaa gcggatcacc atatctgcta tgccggtaaa atgtctcttg ggcgctttct
     2701 ttctgttgtt ctttgtacag gtggtgctgt ttggcagaag ctttgtgttt gcggaaggca
     2761 caggagtgca ggatcttaac gcgcaggtga cgaataatga gacattaaaa ggtgtgtgga
     2821 tgagcgagga acgggccgga tggatgcagg ggatctcaga gtatgtgaat gaacggggac
     2881 ttgccggaag agatgtactg atctacggac agatcccggc gctgtcttat tatttacaga
     2941 tgcctgcagc gttcaatccc tggccggatc tggattccta ccagagcgga caactggaac
     3001 aggatatgct taagatgcag gagcggatgg atgcggatgc ttcttatcgc ccggtggtac
     3061 tgttggagaa gaaatatgcc gtgtatctgg aagctggaga gaatgcactg gaggccttgc
     3121 agcccacgga aaaagagcgc ggcctgatcg tggacaatcc caagcttttg ctgatcggaa
     3181 aattcatgga agattatggg tatgagaaga cttttgaaaa tgagaaattt gtgatctatg
     3241 aatagagtca tggagttgtg cagggtcagc cgtgtgcaga aaaatccccg gaagaaaatc
     3301 tccggggatt tttgagtgtg tgcgcctagc ggcgcacgtt tctaacgggt taaagtcccg
     3361 aatccgccc
//
LOCUS       DAWDHD010000226         3361 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_1698_length_3361_cov_12.808832, whole genome shotgun sequence.
ACCESSION   DAWDHD010000226 DAWDHD010000000
VERSION     DAWDHD010000226.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3361)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3361)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3361
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1698_length_3361_cov_12.808832"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          826 a          888 c          707 g          940 t
ORIGIN      
        1 taaatgcttc aaaaatcact tttactctga aaggatatta tatatgagtt taaagttttt
       61 ggcaagttta ttcatattta ttgttttcgt gtataccatc cttgttgtgg cacagaatac
      121 acgtcgtcag cgagccctta ccgagaagaa agagcaggaa ttctgggacc gggaaaaaaa
      181 ggccaactct gtccgcaaaa aaccccttga tggtctggat tatgtaaaga ttcctctgga
      241 aaaacttccc atgaatgctc tgtcggagga tgaaaaagcc cgggaatata aggagctttt
      301 gacctatcta tccacacagc cggtcgtaaa tctcacaggc ttcaccaaca ccgacctcaa
      361 gttggaatac ggcactgcga acattacacc gctgtcccag tatgatcaga attacaccgt
      421 actggtacgt actctgcagc aatgggccga tctgcttctg gaatccggtc tgacagaaga
      481 tgcggaaacg gttctcgcgt atgccgtctc catcggcacc gacgtcagcc atacttacta
      541 tgcgctggct aaaatctatg ccggccggga ggaatatgac aaaattgccg atctgatcct
      601 ccgtgcggaa gaactgcgtt ccgctttacg taattctatc gtccgtactt tgcaagaatc
      661 ttatccgcaa gcctgctcgc atcactgcgg gtaagctttt ccacatcgac ctccaaagtt
      721 atcttatgct gctctgccag acgatataac cgctcttttt gcggtttcgt cgcgggcgta
      781 tctcttttta cctgaaaatg taatggctgc ggctcaaaca gattattgtt tgctttcttt
      841 tcgacggatt ctgtcgatgc ctgtccaccg gtttcctcct gataaaacaa ttctgccagc
      901 tttcggaaca accggtccgt ggctttcgca tcctccagag cccggtgtgc atgatgcggg
      961 atctcataat actgacatag ataccccaga ttacgatgag gcagttccgg cagatatttc
     1021 cgggcgacct gcaaagtgtc caccgccgat ctctcaaaag taatcttccg gtccactgcc
     1081 gcttttttca aaaaagaata atcaaacaaa attctgtgtc ccagcaacgg taattctccc
     1141 ataaattcta ataattgggg gaaaacctca tccagctgag gtgcatccgc aaggtcttcg
     1201 tcccggattc ctgtcagttc tgtgatccgt tcttccaatt tccgccccgg atccacaaac
     1261 gtagaaaatt cttccgcaat ctgtccctgc tccacacgga tcgcaccgat ctctatgatc
     1321 cgatctctct tgggattcag gcccgtggtc tccaaatcta tactgacata agtgtcatac
     1381 atactatttt cttatccttc cacagtactt ctgatatatt ctgactgcct acctcaattc
     1441 ttatgaaccg agctcctcct gtacttatag tatcggatac ccgatcactt gtaccttggc
     1501 ttcataattg agcggattcc gctcctgata atcaaacaac acgtaatggg gttccggata
     1561 tcgaaacagt tctaagtgtg tcattcggct taactgtttc cagtcatact gatcataggc
     1621 agaaagataa tgtctctcat gctcttctgt ctgataaaag cggcgaatat cctgtttttc
     1681 ctcttccgtg atctctgccg caaacgcagg cctgctcttc agattctccc gaaggtacat
     1741 atcataagtc agaagttctc tgtaaatctc acgattggcc ggatctttca tcgctgcgaa
     1801 ctccaataag atctgatacc ggtaggctct ggcggggctg ttggtaaaat atccctgttc
     1861 ctgataataa tccgccagtg cctcatacat ggcaaaggga ccggggtatg ctttttccag
     1921 aaccggcagg gtatgggtga actgcccgct attgtaatac agctccacca tttcttcgat
     1981 ccgtttcagt ctccggatct cgccatagct gatccagttg gtatacagca cttcatacgg
     2041 cggctgttcc agataacgga ttccgtattc ctgtgcatgc acatgcatat cggagccttt
     2101 taatactttc aggaaaccca gctgcagctg ctccggctgc atcccgtaga cccggtcgaa
     2161 ggatctgccg aaactctcat aatcctcata gggcagcccg gcgatcagat ccagatgctg
     2221 atgaatattc tgtcctctgt gaatggccgc aacaattttc tccagcttgt ccacatccat
     2281 gacacggtga atagctcgta ttgtctccgg attcgtagac tgcaccccga tctccagctg
     2341 tgccagtccc ggacggaagc ggttcaacaa tgcgatctct tcttcgcgca aaatgtccgc
     2401 agaaatctcg aaatggaagt tagtgacacc attgtcatgt tcgtaaatat accgccagat
     2461 ctccatggca tgtttgtggt cacagttaaa ggtacggtca ataaacttca cctgtttcac
     2521 gttttggtcc agaaaaaact gaagttctct ctttacgaca cttatgtcac gtagtcttac
     2581 cttcttatct atggaggaca gacaatagct acaccggtag ggacatcctc tgctggtctc
     2641 ataatagatg atccggttcg taaagggctc catatcctca tagagaaaag gaagtgtagt
     2701 cagatctgtc aggtctctgg gtgccgtata accggaagca agacataatc ccggaatctg
     2761 tccgaaacgt tctaccatag actttcctgc aaccgttccc cttcggtctg ctacctgatg
     2821 ttctgtgctg tcggattcgg gttgttgttc agactgtccg gccgtttctg cctcccgcag
     2881 atattgttcc agtacttccc ggaacgtgac ttctccttct cccaccatga ttccggtaac
     2941 ctgagggaac tctcttaaaa gccccggacc gtcataggta acctccggtc ctcccagcca
     3001 gatctccgta tccggcagga tcttcggcag ttccgtcaga atttcccgga tcagcttcca
     3061 gttccagata taacaggaaa atcctattac ctttggctga cgcttccaga tcccctccag
     3121 aatactttcc agagactcat tgatggtgaa ctccgccagt tccacatagg gctgtaactt
     3181 ttctcccgca ccggcccgca ggctgtatat ggcgggattg gaatgtatga attttgcatt
     3241 aatagctgtt aataagaatt tcatgggact attatactaa gaaaaaagaa ttgtgtcacc
     3301 acaattcctc agactgtaga caaaacgctt ttggtcagca ctccagaagc gtttttcttg
     3361 t
//
LOCUS       DAWDHD010000227         3305 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_1749_length_3305_cov_10.221231, whole genome shotgun sequence.
ACCESSION   DAWDHD010000227 DAWDHD010000000
VERSION     DAWDHD010000227.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3305)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1749_length_3305_cov_10.221231"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          866 a          888 c          600 g          951 t
ORIGIN      
        1 ccatggccat ctgtgctgcg atctctccgg acttatacat ggataccaca ttaccgccca
       61 gcagtccgtc accgattccg gcctccacca tgcggtaaac cggtacattg gaataattca
      121 cgaccatacg gaccgcctgg tcactggtat attgtttgcc actgccgtcc tttgacataa
      181 cgatatagat cagtatcgtc ttatcatcca ccttgctgat agcctgctgc aatctggctg
      241 tttccagttc cgcagcattg atctcagaaa attccagttc cggatagttt tccgctgagt
      301 tgtaaaaatt cttccgttct gcatctgcgg tcactgaatc atccagtatc gccactactt
      361 tatcggctgt cggattcacc ttcagtgcca tgtctatgtt cttctctacg gacagttttt
      421 caataattcc cgtcacaaga ggattctctg ccgctttcat ggcatattct tcatcgttca
      481 caccttcgaa gacaaccggt atctccggga acagttcttc ccggtattcc atggcaaact
      541 gcagcgccgc atcatctccc acgatcacca catcataggg atccgtattc tccagatggt
      601 atttcagcat atcatgaaac atattcagcc actcatctgt ccgaaaacgc ttcgtgtcca
      661 taaattcata gtcgatagtg gtattctcat ccactccggc tttgattcct tcgatctgtg
      721 tctgcaccgt atcccatccg taagaatagg agctgataaa cagtacccgc tttccctgat
      781 tcagattctg tgcttctgcc ttcattgaca ccgtcccaat agtcccaatg cctatgagaa
      841 cagccaatgc aaacagccat gctttccact ttctcatatt ctgtttcccc ctaattccaa
      901 cattatgcta tcggggtcaa aagtctgaac tcaataccac gatataagga attacatcac
      961 tggtactatt gtaccacata attggctgaa agcaaagagc ttaatttaat cttacgataa
     1021 taccttcttc ttttttctct ttcatttttc cgataacggt agggattgct ccgagcgcca
     1081 caaacagata ctgtagaacc agattgccta tgtagcttcc catatcaata atttctgcgg
     1141 aaagtgccgc agggatcagt cgataacact cgaacagact ataacctgct tccgcaccgc
     1201 cgccctgatt gaacagaatg atcgcccagt ccacacgatc tgcgaaatac gtcataagga
     1261 tcatgatcac agcgctgatg accacagcct ttttggtcag ttttccgccc agcatctcat
     1321 atcccttcag aacgcacacc gccatgatca caccggataa tgctgctaca tatcccaact
     1381 ggcttaagat cagaacactg agcattccca acagggatcc taacagggca cctacaatac
     1441 cgcctacgat gttttctttc ttctgcgcct tctgctgtgt cttcattgcg atgtcactgc
     1501 gcatcttcgt ctcgcagtcc ggacataaat gatagtattc accgcccatt ctgaacgctc
     1561 cggtctctac attctgaccg cacagatcac agcaggggct gtaactgttt gtacgcagga
     1621 atgtcgttgt agctgcaagt gcttcgttca atgtatcctt acatttttcc gcattggact
     1681 gtgccttcag tgatactctg atatccagat tcttctgacc aaaactcgcg atcttcttgc
     1741 tgcttttctg aaaccctttc actgcctgct tgtcaaatgt cgacccgtca gcgctttttg
     1801 ccgccgtatg cagcaccatc atatatggat atcttgcatc gggtgcatag acgataaagt
     1861 caaacccatc ccgttcccca tgaaacaggc cacccacttc atcataacgc aatccggttc
     1921 ccatggcaat ctgctgataa ttttcaatct tctttttctg attcattttt ctcccatcct
     1981 ttgtaataaa aaattagtaa tcgctctaaa gcgttacgaa aatcacactt ttccatttta
     2041 cactttaccg tgttaacatg caaccctatt agggggtttt tagccgatta acctatttgc
     2101 tatgaaaaaa ctgtaggcac atctgtgtaa cctacagttt ttgaacttcc ttataaatag
     2161 aacaaggtaa tcgaaatcac gcaaatacgg atttgcattc catcagcatt tgatttcaag
     2221 gtaacctaac agcttgctca tatcgtactc ttccagcaca cccagattgg aatcatagaa
     2281 ttttccatca tagtgtacca ggtaatgaca gaaacgtccc atcttttcca tcatgataca
     2341 gcacttcggc agtacagcgg gtctaccctc ggtatatacg atgatgtcgg tatggtcaat
     2401 gccgtaatag ttcagtgcag agatcacgcg acccatggta gcctgccact ctctgcagtc
     2461 catcacgctg atcacctctg cgatagtaac accggctaac attgcgacac aagcctgacc
     2521 gcacagaccg tcttcgggct ggatgatcac gttcatgctg tcgatcttgc ggattggatt
     2581 ctcaaagctg taagggctgt tctgatccat acggatcagg gaaccgttaa tatgtaacag
     2641 gatcttgtga ggcacaccca gctctacttt tacagcagct tcatcggtga tcacgatctc
     2701 gtaattgccc ttctccttgg gatgaagctc gcactgaatg atattgttat ccagaacgat
     2761 atctcccttg atgacttctc tctgatggca tacttcccat acattaaaag gaatctgaat
     2821 ggtgtatcct gcatcctttt tctctacttt agattcaaaa aaatatctca tgattcattc
     2881 ctccgttgct ttagctgttc ccgaatattc tcttaagaaa aagatttacg aaagcggtta
     2941 cattttctaa tttccttgat tctagcaaaa atttccgccc ttgcaaagag cttgtggaaa
     3001 agtacttaaa aaaaaacatc ctgtcaaaaa aatatcaaaa aagcgcccgt tttaaaacag
     3061 acgcttttta taaatccagt gcatttaaag ccctaaaatt gcagatgcaa cgcggaaata
     3121 tactaacagt gacattgcat ccacgatggt ggtgataaac ggacttgcca ttaccgccgg
     3181 atcaaagccg agacgctttg ccaggatcgg cagaaggcat ccaatgatca ttgcgatcac
     3241 taccgctgct accagagtca ggcacacgga tgctgccacc agcaccccta ctttatccaa
     3301 taaca
//
LOCUS       DAWDHD010000228         3290 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_1760_length_3290_cov_12.890263, whole genome shotgun sequence.
ACCESSION   DAWDHD010000228 DAWDHD010000000
VERSION     DAWDHD010000228.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3290)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3290)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3290
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1760_length_3290_cov_12.890263"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          746 a          906 c          708 g          930 t
ORIGIN      
        1 aatctacgaa ccgtgtcagg ttgggaacaa agcagcacta agtagaacct tccatgtgcc
       61 gtgaggaaac tgggtggagc tgttatcctg atccggcatg tgcggttttg atatttaaag
      121 tgttctgtga attcatggat aagtcagtta agaggttata gattgtgtca ggcgatatgt
      181 catccccttt ttcggtttcc aacagcatag catccgtttc cggcgatctg gctccccaag
      241 atgccgcatg gttccggaaa atcatgtccg actgcatcct gtgtcccagg gcctgtcatg
      301 ccgatcgcct ggatggtggc gtgggatatt gtggccagac ggcagatatt atggctgcca
      361 gagcctctct gcattattgg gaggagccct gtatctccgg tacttccggt tccggtaccg
      421 tctttttctc cggatgcaac ctgcgctgtg tcttctgcca gaaccataac atcgctctgg
      481 ggaaagccgg acgagtcatc actcccgaac atctggtgaa aatctttcta caattacagg
      541 aacagggtgc taacaatatc aatcttgtta cccccacaca ttttcttccc cagatcgtca
      601 ttgccttaca gcaggccaaa aatcagggac tgtccatccc cattgtatac aataccggca
      661 gttacgaatc tccggaggct ttacgccatc tggatggtct ggtggatatc tatctgccgg
      721 atctgaaata tttttctccg gagctttccg ctgcgtattc gcatgccccg gactattttg
      781 agattgcctg cgctgccatt gcggaaatgt accggcaggt cttagatccg gtcatggatc
      841 ccgacaccgg gctgatgcag cgggggatga tcgtacgcca tctgctcctg ccgggacaag
      901 cgaaagactc caagaagatc ctgcggtatc tccatgagac ctacggggat catatctata
      961 ttagcattat gaatcaatat acaccacttc cgcaggtggc agatattgaa gcactcaatc
     1021 gcacagtgac accggaggaa tatgaccggg tcctgcgctt tgcggagcga atcggcattg
     1081 aacagggctt ccggcaagag ggaagtgccg cctgtgaaag ctttattccg gaatttgacg
     1141 agcggggact gtgattctct cacagttcca ctgcgatcag ataatatccg aaatcccctt
     1201 cccatgccgg ttctctggtt ccttcaaaac tttcaaagcc aaacattctg gctacctgtt
     1261 tttctctttc ataaaaatta cccgggactc ctatgtaata tttcacttcc cctgagactt
     1321 cctgacgcat taataataaa tgtctgtaat tataatagcc atgcaacaaa aaactgttgt
     1381 gggtcatctg atagcaacgc tcccgcaaca caacgaaatc ctccggaccc acggaaagaa
     1441 attccctggc atcccggaag ggatggatgt gggggtagat actgcttaat tgctcccatt
     1501 tggtgtcttt catgtggaga gtgtgttccg tggcagtttt tttcgagctc cgggcttccg
     1561 attcactgac cattttttcg tttgcgacta cggtttctgc cactttttcg gtctgttcac
     1621 gggcttgcct gccttgctcc tgcacccgct cccgctgcat ctccctattt tgagtttcct
     1681 gctgttctgt ctgtatcggc cgctttgcct gctccggctg gtttccacct tgcccctgtc
     1741 cttcgatttc ccccaccgta accggtgcaa tcgcagtcac aggcgcaggt actttcttcg
     1801 caccagccgc tcctgcaaac cgccctatga tctcaaaatt acgttccagg ggaatgcgta
     1861 tccccagtgt cggctcccaa tccgcttttc tggaaattct tttttcacag gaaatggtcc
     1921 cccgttctgc cgtcatttca cataacagtt cttctccgct ttcccgctgc agatacagca
     1981 gcatcatctc cttgccagac acgatcccct gtcctctcag ctgcaccatc agaatctctt
     2041 ccttttccca gctttccagc tttgcatacc cggctccccg gatccgttgc ccgttttcct
     2101 gtaattccaa atagctgatc tgtttccgat atgacataaa atccccccgt ataaagtttc
     2161 tatctaactt tatgcgaggg gttgtcctta atattcctgg ctgtccagat atttcatata
     2221 atttacgacc atcaacgcga tcttgaaagt cagtgcatca tcaaagctcc gcagatccag
     2281 gccggtgctc ttctgaatct tctcaatacg atataccagc gtattacgat gcacgaacaa
     2341 ctgtctggaa gtctccgaca cattcagatt gttttcgaag aacttgttca gggtgttcag
     2401 ggtctcttca tccagatcca tgggtacatt gtcaccgaag atctcctcaa taaagatctt
     2461 gcacagattc accggcagct gatagatcag acgtccgatt cccaacgtgc tgtaggcgat
     2521 cacctttttc tctacgtaga agatcttgcc tacatccagt gccatcttgg cttccttgta
     2581 ggacttggat acgtccttca attcctgtac cacggtgcca tatgccacct tcacatccag
     2641 aagagcctcc gcattcatca tggctacaat agtctcggcc agttcatgca gtgtctcagg
     2701 tgtggtggtg ctctcaacag atttgatcag aatcaggctg gactcatcca ccgctgttac
     2761 ataatctccc gcctgcgggg aaaacatgcc cttcagcact tcagatacaa tgccatcttt
     2821 ctcatccttg gtctcaatga gatatacggc tctgggacag ctgacttcca catgaagttt
     2881 cttcgcccga ttgtaaatat ctaccagcaa cagattatcc aacaacaggt tctgaaagaa
     2941 attattccgg tcaaaccgtt ccttgtaggc aatgacaaga ttctggatct gactgacagc
     3001 aatcttaccc accatatata cgtcatccgt cattccccgt gccaccagta catataaaag
     3061 atccccttcg tcacggatct tcagaaggtg atgtactccg atcacctggc tatccgccgg
     3121 agaagccgca aagcctgtga tcagatctgt ggttatgtca tccttttcca tggtagatgc
     3181 cacttcggat ccgttcagat catagactcc gaggtctacc ttagtgattg cttttaattc
     3241 atcaatgctt gtctgtataa tctggcctgt aatcatattg tccacgcctt
//
LOCUS       DAWDHD010000229         3260 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_1785_length_3260_cov_9.892356, whole genome shotgun sequence.
ACCESSION   DAWDHD010000229 DAWDHD010000000
VERSION     DAWDHD010000229.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3260)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1785_length_3260_cov_9.892356"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          926 a          673 c          865 g          796 t
ORIGIN      
        1 agaattctgg gaggaatatg tagaagatgt atcgattgat taaacaggaa ggacgtgcca
       61 agagaggcga atttaccact gtacacggcg tgatccagac acctgtattt atgaatgtgg
      121 gaactgtggc tgccatcaag ggcgctgtgt ccacagcaga cttagagcag atcgggaccc
      181 aggtggaact gtccaatacc tatcatttac acgtaagacc gggagaccag atcatcaagc
      241 agatgggcgg ccttcataaa tttatgtcct ggaacaaacc gatcctgacg gattccggtg
      301 gatttcaggt attttctctg gcggggctgc gcaaaatcaa ggaagagggc gtaactttcc
      361 gctctcatat cgacggacac aagattttca tgggtccgga ggagagcatg cagatccagt
      421 ccaatctggg atctaccatt gccatggctt ttgacgagtg tgcccccagt aaggcagacc
      481 ggatgtatgt acaaaattcc gtggaacgta ccaccagatg gctgcagaga tgtcaggcgg
      541 agatgaagcg cctgaacagt ttagaggata cggtgaaccc acaccagctg ttattcggca
      601 tcaatcaggg ggctatctat gaagatatcc gtatcgagca tgccaagcgg atcgctgaga
      661 tggatctgga cggttatgcg gtcggcggtc tggcagttgg cgagacccat gaggaaatgt
      721 accacattct ggatgaggtg gtaccttatc tcccggtgaa caagccaacc taccttatgg
      781 gtgtgggtac cccggctaat attctggagg gtgtagaacg tggtgttgat ttcttcgact
      841 gtgtatatcc gacgagaaac ggacgccatg gacatttata taccaaccat ggaaagatta
      901 atctgtttaa tgccaaatac gaactggatg gcagacccat cgaagagggc tgcaactgtc
      961 ctgcctgcca gagatactcc agagcttata tccgtcatct gttaaaggcg aaagagatgc
     1021 tgggtatgcg tctttgtgta ctccacaatc tgtattttta taataccatg atgacggaga
     1081 tccgtgatgc cttggatgca ggccgttttg cagaatataa aaaacagaag ctggattcca
     1141 tggctacacc gttagaataa gttacaactg tcggagttta gaggaatttc atacaatttt
     1201 atgcaaaaag acttgcccct taagctattt ttgtgtatca tacttacttg tacagaaagt
     1261 tttacaggcg taacacgccg gaatggagga acaaatgggt attttattaa ctgagtcaga
     1321 ggctgctgca gcaggttccg gaaccagcat gatcgttatg cttcttgtat atgcagtatt
     1381 gatcggtgca atgtggtttt tctttatgag accgcagaag aaggaacaga agagagtagc
     1441 tgcaatgctg tccaacttag agacaggtga ctgcgttctg accacttccg gtttctatgg
     1501 catcgtgatc gacattaccg atgataccgt tatcgtagag ttcggtaata acaagaactg
     1561 ccgtattcct atggagaaat ctgccatcag caaggttgag aaggctggag agaactaaga
     1621 agaagtgaaa aaggagcaat gccgggataa ggtattgctc ctttttgcat atacggcaca
     1681 actaatggct tgatctattt taatgtccat aattgcattt atgttaattt tcaatgttga
     1741 aatttagtac tgtatcagtt attatgaaat aagtatttta cgtaaaaata caggaaatat
     1801 cacaggaaac gaggaaaata acactatgga gttacatatt acctgtattc ccggcgatgg
     1861 cattggacct gagatcgtca gggagacaaa aaaagtcttg gacaaagttg cgcataaata
     1921 tggtcataaa atgatctata aagatattct gatgggagga gcttccattg atgttcatgg
     1981 agtccctctg acagaggaag cgattgcgga tgccaaagca gcagatgcgg tgctgatggg
     2041 atccattggc ggcaacacta ctacctctcc ctggtacaag cttcctcccg agaagcgtcc
     2101 cgaggcggga ctgctggcac tgcgtaaggc attgaatctg tttgctaatt taagacccgc
     2161 agtactttac gatgaactgg cagcagcgtg tcccctgaag gaggagatca gcaaagccgg
     2221 aattgacatg ctgatcatga gagagctgac cggcgcactt tacttcggtg agcgtaagac
     2281 tatcgaagag aacggtgtga gaaaagctat tgataccctg acctacgatg agaacgagat
     2341 ccgtcgaatt gctatcaaag gttttgatat tgccagaaag cggaaaaaga aagttaccag
     2401 cgtggataaa gccaatgtcc tggattcctc cagattgtgg agaaaggtcg tggaagaggt
     2461 cgcgaaggat tatcctgacg ttgctttgga acatatgctg gtagacaact gtgccatgca
     2521 gttagttaag gatcccgcac agtttgatgt gatcctgaca gagaatatgt tcggagatat
     2581 cctgtccgat gaggcaagta tggtgaccgg ttccatcgga atgttagcca gtgccagctt
     2641 gaacgataca aagttcggtc tctacgagcc cagccacggt tccgctccgg acattgccgg
     2701 taaagatatt gcaaacccta tcgccactgt tttaagcgcg gctatgatgc ttcgttacag
     2761 ctttgacctg gatgaagaag cagatgccat cgaggcagcg gtgaagaaag tattgcagga
     2821 cggcttccgc accggcgata tcatgtctga gggctgcacg caggtaggct gtaccgagat
     2881 gggcgacctt ctggcagaga gaatctgaca acaattaagc ggttacattt gcgaatagag
     2941 aagagaaaga gagacaaaga tatgagaagt gattctgtaa agacaggaac tgcccaggca
     3001 ccccacagaa gtctgttcaa tgccctggga tttaccgaag aagagagaag acgccccatg
     3061 atcggtatcg tcagctcctt caatgagatc gtacccggac acatgaatct ggataagatc
     3121 accgaagctg taaaaatggg cgtagccatg gcaggaggca tgccagtggt attccccgcc
     3181 attgctgtat gtgacggtat tgccatggga catgtgggaa tgaagtattc cctggtgacc
     3241 agagatctga tcgccgacag
//
LOCUS       DAWDHD010000230         3260 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_1786_length_3260_cov_8.561934, whole genome shotgun sequence.
ACCESSION   DAWDHD010000230 DAWDHD010000000
VERSION     DAWDHD010000230.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3260)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1786_length_3260_cov_8.561934"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          766 a          847 c          668 g          979 t
ORIGIN      
        1 gtcggcgatc ttgctggccc ccaggattcc ctccatgccg ttctggtcgt cgaaaatttg
       61 cgtgatggct tccatctctg agaaagaaag agtgctgact gcagatttta cgacagccac
      121 cttacggctc tcctccgcgc tctcttcatt cacagcccgc aacatttcca gtcccaccac
      181 tgtggtacca tattcgcaaa ggataatatc atcaatatca tactgttcat tgcatttgta
      241 aatgaagagg gttcccaaac gctctccggc aatctcgatc ggagtgatga tcgcctgaaa
      301 ttttctcgta tcggtatttt caaagccaag agtctccagg ttgacgttct ctttcgtgga
      361 taatacgccc agcagtctct cgtttaacat cggatcaata aatccaccga tattgtcatc
      421 cagcagttct gtgatcggct caattccgtc ggattttcct acgccgagga cctttccttt
      481 ccggctgatc accagtacat tggaattcag aatttcccgc atcaccttac agatgtcgct
      541 gaataccact ttgctggagt tattgttgtg taataatttc ccgattttac gtgttttgtc
      601 caacagttgt acgctactca tctatttccc tccgtctaca catactaaga aactaccaga
      661 tgttatgcac attgtagcac atgtaatttc agattacaat gaaaatagcg ttaatttatg
      721 agaaaattct gacagtatat aaaactactt ctcttccgtc ggtttatttt tcacatagcc
      781 gcactgttcg tccatgcaga ccagtttgtt gcctttttcc agcagcagct tgccacagcg
      841 gggacacttc tctcccgaag gcttctgcca taccatgaaa tcacactccg gattatcgat
      901 acagccataa tattttctgc cttttttggt ctttttcatg acaatatcct tgccacactt
      961 ggggcaggct acaccgatct tttcaaaata aggcttggta ttacggcatt ccgggaatcc
     1021 aggacatgcc aggaatctgc cgtggggtcc gtatttgatc accattcgtc tgccgcacag
     1081 ttcacagacc tcatcggatt cctcgtccgc aatggtcacc tctgccaggt ccttctccgc
     1141 tttttttact gcttcatcca gatcaggata gaaattggac acgatggtct tccattttac
     1201 cgtgccttct tccacaccat ccaacagagc ttccatgttg gccgtgaaat tcacatccac
     1261 gatggagggg aatgcttcct tcatcatatt gttgaccact tcccccagtt ccgtaacgta
     1321 gagattgcgg ttctccttgg caatatatct cctggcaagg atggtggtaa tggtaggcgc
     1381 ataggtactg ggacgtccga tgcccagttc ttccatggca cgcaccagac tggcttcggt
     1441 gtaatgtgca gggggctggg taaaatgctg tttcggatca aagctctgga attccagcac
     1501 gctgttctcg tccagattac cgccgagcac attttcttct tccttatcat cttcctgggt
     1561 gtagacactc atgaatccat cgaaacgcat tctggaagct gccagagcaa aaatctggtc
     1621 ctttgcctga atcttcacag aagtggtatc atactgtgca gcactcatgc ggcttgccac
     1681 aaatcgtttc caaatcagct gatacagacg gaacagatcc ctgggaagct gctccttgat
     1741 agcaataggc atccggttca gatccgtggg acggatagct tcgtgggcat cctgaatctt
     1801 ctggctgttc ttcttctccg ttgctgcagc ggtcttgtat ttttcaccgt aatgaacatc
     1861 aataaattcc gctgcctgtg tctccgcctc ttcggataca cgggtagaat cggtacgcag
     1921 gtaggtgatc agaccaacag tgccctctcc cttgatatcc acaccttcat ataactgctg
     1981 tgccagacgc atagtcttct gggtagagaa atttaatacc ttgctggctt cctgctgcaa
     2041 tgtggaagtc gtaaagggaa gcggcgcttt tttcacacgt tcgccgtgac gcacttcttt
     2101 tacttcgaat tttgcgccat tcagggaatc cataatgctg tctgcctgtt ccttgctggt
     2161 aattccctgt tttttttcgg tggtaccgta atattttgca caaataggct tcttctcgcc
     2221 ttttactttc agattcacat ccagagacca atactcttcc ggaataaatg cgttgatctc
     2281 gtcctctctg tcacagatga tacggagtgc cacagactgt acacggccgg cacttaagcc
     2341 tctcttgatc tttgcccaca gaagtgggga gatccgatag cccaccatac ggtccagcat
     2401 acgccttgcc tgctgggcat ccaccagatt catatcgatc tctctggcgt ttttcagaga
     2461 ttcctttacc gcattcttgg tgatctcatt gaaggtaata cgatatactt ttttgctttc
     2521 cagtttcaag gcataataca gatgccagga aatagcttct ccttcacggt ccgggtcggt
     2581 tgcgagataa atcttatctg ccttttttac ttctttgcgc aaagaagcaa gaaggtctcc
     2641 ctttccgcgg atggtaatat atttcggttc ataatcatgt tccggatcaa atcccagctg
     2701 actcttgggc aggtccctca catgcccctg gctggccata acttcataat ttgctcccaa
     2761 aaactttttg attgtcttca ccttggccgg tgactctaca attactaaat attttgccat
     2821 atgcactccc atctatccgt ctgtctacgg acctacggca tccggttccc gcctctttgc
     2881 cgtattttcc cctgctttga gtaatcgtga accactatac aacaaaaaaa tgggaatggc
     2941 aagaagattt ttcatttgcc cagccccaga cagtataaaa aaagagccaa aaagcaattt
     3001 tctatgcttt ccgacacttt tcagggttgg gctattctat taaagaataa gcagctgata
     3061 ggggaatcga acccctgttt tcgccgtgag agggcgacgt cttaaccgct tgaccaatca
     3121 gccataggtt acttacgaac cttgttcagt ataatacatt cttttgaaaa ttgcaagtat
     3181 tttttgaaaa atttctacaa ttttcaataa gcagctgata ggggaatcga acccctgttt
     3241 tcgccgtgag agggcgacgt
//
LOCUS       DAWDHD010000231         3231 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_1805_length_3231_cov_13.367758, whole genome shotgun sequence.
ACCESSION   DAWDHD010000231 DAWDHD010000000
VERSION     DAWDHD010000231.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3231)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1805_length_3231_cov_13.367758"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          745 a          847 c          781 g          858 t
ORIGIN      
        1 ccgatacgga aatcagcact cagggaagcc acgccgggac ctacgaattc cattacttta
       61 ttttttacat agccattctt gaatactgcg ccaatgatag ccagtgccac gtcctgagga
      121 cctacaccct tcacgggagc accctttaag tatacggcta ccacgccggg catgttgata
      181 tcataggtct ggttcaacag ctgttttacc agttcgggac caccctcgcc catagccatg
      241 gtacccagtg caccgtaacg ggtatgggaa tcggaaccca ggatcatctt gccaccgccg
      301 gctaacatct ctctggcaaa ctgatggata actgcctgat gagggggtac atagactccg
      361 ccgtattttt tcgcacaggt cagaccaaac atgtggtcat cctcgttgat ggtaccgcct
      421 acggcacaca gggagttatg acagttggtc agtacatagg gcatggggaa cttctgcagt
      481 ccggatgctc tcgcggtctg gataatacct acaaaggtaa tatcatggct ggtaagcttg
      541 tcgaaacgga tcttcagctt gtccatattg ccggaggtat tatgctcctg cagaatacca
      601 tatgccatgg tgtttttcgc tgcttcttct ttggcgatcg taatgccggt cttcgcctgt
      661 atctgactgg ctgcttccgg ggtatccgct acgatgtccg taccgtttac caggtaagcg
      721 ccgccctgtg ttaactgaat catgctcgtc ctcctgttat tccacttctt atgcgtaatg
      781 tgcatctaaa tgtgcacttc tttcattata ctggattgtt ttctcagatg caatgatctt
      841 ataggatgtt tttgccactg taccacgcta aattttattt ttctccggga accattcctc
      901 ttttgttccg gtgttgctat aatatcctca gaaattatgc cattttccga aaatctatac
      961 gaaaacgaca gttgtcggcc gaattccggc ggcagcggaa aggaacctta tgaatgtagt
     1021 aatagctatc gattccttca agggaagtat gacctccatg caggcgggtt tgagcgccgc
     1081 cacggggatc aaacgtgttt ataaagatgc acatattacc gtccgtcctc tggcggacgg
     1141 cggggagggc accgtcgatg ctctggtaaa cggctgtgac ggccgtatga cacaggtgca
     1201 ggtcacaggc ccttccggtc accccgttgt ctgtccttac ggcatcgtcg acgagaccca
     1261 taccgcaatc attgaaatgt ccggtgccgc cgggattacc caggtcagcg gagaggaaaa
     1321 gaatcctctg aacaccacca cttacggtgt aggcgaagtc atcaaagatg caatccagaa
     1381 cggctgccgg cattttattg tcggtatcgg tggcagtgcc accaatgacg gcggtgtcgg
     1441 aatgctgcag gcacttggct ttggctttct ggataaaaac ggaaatcaga tccgcttcgg
     1501 agccaaagga ctggaggatc tggaatccat cactacggat catgtgcttc ccgaactcaa
     1561 ggaatgtact ttccgcatag cctgcgatgt ctccaacccg ctctgcggag agcagggatg
     1621 ttccgccatc tacggaccgc aaaagggcgc tacttccact atgatcctgc agatggacaa
     1681 atggctggct tattatgccg ctctggccag agaacagttt cctcatgccg atgccaaata
     1741 tcccggtgcc ggtgctgccg gcggtatggg atttgctttt cttaccttct gcaatgccac
     1801 actggaatcc ggtattaaga tcattctgga agaaacccat ctggaaaaat atatccggga
     1861 tgcagatgtc gtgatcaccg gggaaggccg ccttgacggt cagacggtta tgggaaaagc
     1921 tcccatcggt gttgccaaga ttgccaaaaa atatgaaaaa ccggtacttg ctttttccgg
     1981 ctgcgtggcc aaagaggccg ttgcctgcaa cgcagagggc attgatgcct ttttcccgat
     2041 tctgcggaac gtggtatccc tggatgaggc tatgcatacc gacaatgcca tgaccaatat
     2101 ggcggatacc gcagagcagg tattccgggc gcttcagatt tttcaccgtt aagaatcaca
     2161 aaaggacatg acccaccggc catgtccctt tgtatgtttc aatattgttt tattattctt
     2221 catatccatt cggattgttc ttctgccatc tccaggaatc ttcgcacatc tctttgatgc
     2281 cattctcagc cttccagcca agttccttct ctgccagaga agcatcggaa tagcaggtgg
     2341 cgatatcgcc tgcacggcgg ggtttgatca cgtaaggaat cttcacgccg gtagctgctt
     2401 cgaagttctt cacgatgtcc agaacactgt agcccacacc agttcccaga ttgtagatct
     2461 tcagtcctgc attctcctca atcttcttca gagcctttac gtggcccttt gccagatcaa
     2521 cgacatggat gtagtcacgt acaccggttc cgtcgggggt atcgtaatca tcaccgaaga
     2581 cacccagctc tttcagcttg ccgactgcta cctgtgtgat ataaggcatc aggttgttgg
     2641 ggataccgtt gggattctcg ccaatggtac cgctcttgtg agctccgata gggttgaagt
     2701 aacgaagcag gatcacattc cattcgggat ctgccttctg gatatccgtc aggatctgct
     2761 ccagcataga tttcgtccag ccgtaagggt tggtacactg tcccttgggg cattcctccg
     2821 taatgggaac aaatgcgggg ttgccgtata cggtagcgct ggaagagaag ataatgctct
     2881 tcacaccatg ttttctcatg acatctacca gggtcagggt accggcaata ttgttctcat
     2941 aatattccca gggcttctgt acggactcac cgactgcctt cagtcctgca aaatgaatga
     3001 ctgcgtcaat ctgttccttg ctgaagatct cttccagagc ttcacggtcc agaatgtctg
     3061 ccttatagaa aggaaccttc ttgcctgtga tggcttctac tctggggatc actttctcac
     3121 ttgcattgca gagattatcc aatacgacta catcatagcc tgcctgctgt aattccacta
     3181 cggtatgact gccgatgaat ccggcaccac ctgttaccaa aattctcata c
//
LOCUS       DAWDHD010000232         3214 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_1821_length_3214_cov_16.063311, whole genome shotgun sequence.
ACCESSION   DAWDHD010000232 DAWDHD010000000
VERSION     DAWDHD010000232.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3214)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3214)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3214
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1821_length_3214_cov_16.063311"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          795 a          870 c          699 g          850 t
ORIGIN      
        1 tgtcaacctt cagagtggtg ctctccttgt aaggacggaa gagtagataa atgaagccga
       61 tgatcagaag gaatgctact acagtgccga taccgaaagc accggtggtg atcagcgtac
      121 cgatctgata tacacacagg gataccacgt atgccagacc acactgataa ccaactgcga
      181 tccagaacca tctggtgttg ttcatctcac gcttgatcgc acccattgct gcgaagcaag
      241 gtgcacacag caggttgaat gccaggaaag cataaccgct tgctgcagta aagtgagatg
      301 ccatgttagc atatacggaa ccctcgcctg cgctgtagag gatacccata gtacctacga
      361 tattctcctt agctaccaga ccggtgatgg atgcaacggt tgcctgccag ttaccgaagc
      421 cctgagggat gaagatccat gccagacact gaccgatctt gcccaggata ctcatatcga
      481 tctcatcctc agccagcata cggaagccgt cctcggtgaa tccgaaataa gtagtaaacc
      541 atacaacgat ggtagacaga aggatgatgg tacctgcctt cttgatgaag gaccagccac
      601 gctcccacat gctgcgcagt accgtaccta cggtaggcca gtggtaagcg ggaagctcca
      661 taacgaaagg agcaggatcg ccctcgaaga tcttggtctt cttcaggata ataccggaac
      721 aaatgattgc tgcaattccc aggaagtaag cggaaggagc aacccatgct gcaccgccga
      781 agatagcacc tgctaccatg gcgatgaaag ggagcttagc accacaagga ataaatgtgg
      841 tagtcataat cgtcatctta cggtctctgt cgttctcgat ggtacgggaa gccatgatac
      901 cgggaacacc acatccggaa ccgatcagca taggaatgaa ggatttaccg gacagaccga
      961 acttacggaa gatacggtcc atgataaagg cgatacgtgc catataacca caggactcca
     1021 ggaatgccag gaagatgaac agtaccagca tctgaggtac gaaaccaagc actgcaccga
     1081 caccggctac aataccgtca aggatcagtc ctttcagcca gtcagcacat ccgatcgcat
     1141 ccagaccgct ctctaacagt gcaggtacgc cgggtaccca gataccgtag tctgcgggat
     1201 cgggagcttc tgcgccgtct gctgcgtata catcaactgc ctctgccagc tcagcaccgg
     1261 tgtaagtaac atcctctgtt gccagagtct cttcatcttc cagggtgtaa tctgcggtag
     1321 cggatgcatc gatgcctgct gcgaactcct gaacagctgc tactgctgct gcgggatcat
     1381 agtcttcaga ctcgctgtcg atgactgctg ccagagcttc atcaccgtca gcttctacga
     1441 atgcgttgat cacattcatt gcatcatcat aaggttcggc tgcttcttca taagcaccgg
     1501 ttccgatggt gaacagatgc cagccatcac cgaatacgcc atcgttcgcc cagtcggtag
     1561 cccaggtacc aacggtggta acagatacat aatatacgat gaacataacc actgcgaaga
     1621 tcggcagtgc cagccatctg ttggtcacga ttttatcgat cttatcggaa gtcgttaact
     1681 tctggccttc cttcttggtg tagcattctt tgatgataga ggagatgtat gtgtatctct
     1741 cattggtgat aatactctcg gtgtcatcat ccatagcagc ttccaactgc ttgatctctg
     1801 cggacacatc gggaacactc ttcatctgtg cctggatctt gtcatccttc tctaacagct
     1861 tgatagcgaa gaatctcttc tgttcctcgg gaacatcggt cagcttcttc tctacggtct
     1921 cgatcacgct ctcaacctca ggcgcgaatt tatgtaccgc agctgctgca ctcttcttct
     1981 gtgccagttc gatcgctttt ttggtagcgt tctcgatacc ggtcagtttc agagcggaga
     2041 tctcaacaac ggggcagccc agcttctcag caagctttgc tacgttgatc ttgtcgccat
     2101 tcttgttcac gatatcgatc atgttgacag ccatgacaac ggggatcccc agttccatca
     2161 gctgtgtgct cagatataag ttacgctcga tgttggtacc gtctaccagg ttcaggatcg
     2221 catcgggtct ctcaccgatc aggtagttac gtgctaccac ttcctccaga gtatacggag
     2281 acagggaata aataccgggt aagtccatga tgatcacgtc atcaccgtta ccgccgtact
     2341 gtttctttaa tttaccttcc tttttctcaa cggttacgcc gggccagtta cctacgaact
     2401 ggttggaacc ggtcagagcg ttgaaaagtg tcgttttacc gcagttaggg ttacctgcaa
     2461 gtgcaatctt aattgccatt tttctacctc tttcctctaa aatcatactt ctcataacta
     2521 ttcagagcct cattcacaaa atcgtctctt cgagactttc cttttgctca tgtgattagc
     2581 cttcactaat tagttctggc taaccttcct gttaaaaaaa agatcattta ctcaacgcaa
     2641 atcatctcag cgtctgcctt acgaagagac agctcatagc ctctgacagt tacttccacg
     2701 ggatctccca aaggagcaac ctttctcaca tagatctcaa cacctttggt aatgcccatg
     2761 tccatgatac gtctcttgac aggaccttca ccattcagtt tggttacctt tacggtctgt
     2821 ccgcagggaa cttccttcaa tgtcttcatt ttctcctcct attagcatct tttggtacag
     2881 ctgcccgcta caccatgatt ttattggcca tatccttgcc gatggctact cttgattcct
     2941 tgatattcac aatcaggttg ccttcggtct gggacacaac tgtcacttta ccacctacta
     3001 caaatccgag ggtctctaaa aacttcctcg tctcctcttt gccgccgatc ctctggatcg
     3061 tgttctcttc accggcacct accatactca ggggcatcat acacacttcc ttcccttcaa
     3121 tagtattgaa ctgcttgtgg tttctcatta gctcttaatg agattcattc tcaattcacg
     3181 tggttagtat atactaacct ttttttactg tcaa
//
LOCUS       DAWDHD010000233         3146 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_1879_length_3146_cov_13.426399, whole genome shotgun sequence.
ACCESSION   DAWDHD010000233 DAWDHD010000000
VERSION     DAWDHD010000233.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1879_length_3146_cov_13.426399"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          732 a          872 c          760 g          782 t
ORIGIN      
        1 acgtctgctt acggcctgct tattcttact tgctgtcgtg actactgggt tattcattaa
       61 tcaggaagga aacaccaatc catggcaaat aacatttacc gcgttcctac tatatatagt
      121 gatatcaaaa aaacgactga acctctctca gtccgggtac cgggttctaa gagtatcacg
      181 aaccgcagtc tccttctggc gatgctggcg gaggggaagt ccacgctgcg tggagtgttg
      241 ttctcggatg attcgagaca ttttcttaag tgtgtgcagg atctgggatt tgagacgacg
      301 gtggatgagg agagctatat cgtcacggta gaaggactgg gaggaaagct tccctgtgaa
      361 gagactacgt taaatgtggg aagtgcagga acggcagcgc gttttctgac agctacgtta
      421 ggtgtctcgg aaggtctttt tcacatggat tcttcggagc agatgaagag gcgccccatg
      481 gatccgcttc tgtcctctct ggcagaattg gggtgtgagg tccgctgtga aggagaagat
      541 gggcattttc cttttacgct gaccgctcat gggttcggac aggatcatat ctccatcgat
      601 atcgggcaca gcagtcagtt tctgagtgcg ctgctgattg cttcgacttt gtcttcggaa
      661 gattttatga ttcaggtaga gggtacccac ggtatggcat atatcgaaat gacacggaaa
      721 atgatggagc agttcggtgt cagggtggaa catcccgccg cagatcagtt ccgtatctgc
      781 accggtcaaa agtatcgtgc actggattat cagatcgaac cggacgtctc cgccgcctgc
      841 tatttttatg caatgacacc gttactgggt attcccgtat gtgtggagca tgtgcacttt
      901 gaaagtctgc agggagatgt ggaattctta catattttag agaaaatggg ctgtaccgca
      961 caggagacgg aaaatggggt actcctgctg cctcccgcag gccagacgga taccggaacg
     1021 caggttcctg ccttccatgg catcaccgtg gacatgtctt cctgctccga tcaggccatc
     1081 actctggcag ctattgcgcc ttttgctgac agtcccacct gtattacagg catcggtcac
     1141 atccgtttcc aggaaagtga ccgtatccat gccatctgca cggaactgac ccgcatgggc
     1201 atccactgcg aagagacaca ggacagcatc actatctatc ccggcatgcc gcacccctgc
     1261 accgtagcga cctacgatga tcaccgcatg gccatgggct ttgccctgac cgggctccgg
     1321 gccgaaggta tcgtgatcga tgatcccgga tgctgccgca aaactttcga gaactatttt
     1381 gaagtactgg atcaggtgat tgcgaagata atcgaaatgt aggaatagaa tcatccgtac
     1441 tcacataaac tataagtggc tgcgctgtgt tgcttgtgag gtacgcagcc aggggagccc
     1501 atgcgtgggc tcctttttca cgtacaattc acaaagcatc ccgatatacc cgcatcacat
     1561 ttccccagaa aatcccatcc agctggtttt ccgtaaagcc agctgttttt agtctttccc
     1621 acaataccgg cattgcctcc gcaccgggaa gagccggatt tgtggggatg ccgtcgaagt
     1681 cactgcccag acccagtatt tccataccgc cgacatcagt gatatgtctt gcctgccgta
     1741 tcagtgcttc ccacagcacc tctggatctt tgcttccgcc atccaccaga aatttatcat
     1801 aatagttcag tcccatacag ccgccccgct ccgccagtct gcggatctgg tcatccgtca
     1861 gattccgtag ggacggacat acagcccttg cgttggaatg gcttgccacg aagggcttct
     1921 tcgtacattc ccatacatcg tcaaagccgg catccgacag atgggagacg tccgggatca
     1981 ttccgagacg ctccatctct gtgacaaaat cgcggccggt ttccgtcaga ccacacgctg
     2041 catccctgcc atcggatcca cgtatcccag tctcaccttt ctccgtcagt ctcccatcta
     2101 cgtcgcccct gctctgcccc agacaccgac gcgtggctgg ccaccccagt tcattccgat
     2161 aattccaggt taacgtcagc atccgcaccc ccatgtcata cagttcccgt agcctctcca
     2221 gacttccgcc gcagaccgcc ccttcttcca ccgtcaaaag tgccgacagt ttctcacagg
     2281 cttcattttt caaaatgtct tcatagcaca gtaccgggac aatccggttc ttgtttttct
     2341 ccagctccgt ccggtagacc tcatacagag cacacacccg ctcccagggt gtcggtgtgc
     2401 actttccgat atccacaaac agggcgaaat tctgcaacag atatccgctc tcctgcatcc
     2461 gcttaagatc cagatgaccg ctgtttttcc aaagttcccc gccccggtcc gccagttcgg
     2521 atatggtgtc acaatgcata tctatgatct tcatgttttc tcctgatccc ctatgaaaaa
     2581 gagagcatcc acagctctat gttcctgcca tgaatactcc ctcttacttt ctttgttcaa
     2641 tttatgctgt tgtcagcaaa aaatacctta gttctcttca cccttgactg cgaagcatac
     2701 gccggtatcg tcgggcttat cctcatcgaa cttgtagtcc ttgccgggca ggatagcgtt
     2761 caggataatg ccggtcagcg cgccgactgc cagtccggac aggctgatgg tgatctcacc
     2821 gatgtggaat gcgatagcac ctgctgcact atagttgata ccgatggaca gtaccagaat
     2881 cagagcggcg atgataacgt tacgactctt gatgaaatct acctgagtct caacgatatt
     2941 acgaacaccg actgcggaaa tcataccata cagtaccagg gatacaccac cgcaggtagc
     3001 ggtaggcata caaccgatca atgctgctac cttcggggag aaggagaaca gtacagcaaa
     3061 gcctgctgca atacggataa ctctgggatc aaataccttg gtcagagaca gtacaccggt
     3121 gttctcaccg taagtggtat tcgcag
//
LOCUS       DAWDHD010000234         3101 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_1917_length_3101_cov_10.496717, whole genome shotgun sequence.
ACCESSION   DAWDHD010000234 DAWDHD010000000
VERSION     DAWDHD010000234.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3101)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1917_length_3101_cov_10.496717"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1936..2035
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          859 a          619 c          781 g          742 t
ORIGIN      
        1 tgcctggaaa atagaagcag acaggtcttc cttggtagca ccttcgttga tcagaggctg
       61 gatatcggtt ttggcaaata caccacagcg ggcagcaatg gtgtagaggg atttataatt
      121 tttcgcatat tcgtttaagc ccgaagcgtc cgtctgaatc agactggcca tctggtcgat
      181 gaaggaaccg gtaccgccgg cacagatacc gttcatacgc tgctccacgt taccaccttc
      241 aaaatagatg atcttcgcat cctcaccgcc taattcaatg gctacatcgg tcttgggtgc
      301 cagctcctgc agggaagtag ccacggctac gacctcctgt gtaaagggaa cgccgaggtg
      361 gttggccagt gtcagaccgc cggaaccggt gatcatgggg tgcagctgca attctcccag
      421 ctggtcataa gctttcttaa ggagctcaga cagagtttcc cggatattgg cgaagtgacg
      481 ctcataatcg gaaaataaaa tattgtgttc ttcgtccaaa atagcaatct tgacagtggt
      541 ggaaccgata tctatgccga gagttgcgta tttttcctgt gaattcatgt gatacatcct
      601 tcctgaaata aaaataaatc tacttattta atgcattttt ttcatcaaca aaacattcta
      661 attatacgac aaaaaacacc acaatgcaac gaataatggg ggagtttagt tgttaaagtt
      721 ttgtaaaatg taacttaaat ttttcgggaa aactctgaaa aaagagaacg tgcagtttac
      781 ttattctctt ccggatgtta caatctcgat agagaaaaat caaaatataa ctatttatgg
      841 agacattgtt ttatgagcaa attcgaaaaa aagtttggaa aatatgcgat ccataacctg
      901 accatggtat tgatcatgtg ttatgtggcg ggctatgtga tcgaactgat gggcagcgcg
      961 gccggaaata atctgctggg atttctgaca ctggatcctt accggatact gcacgggcag
     1021 atctggcgtc tggtgacctg ggtgatcgtt ccgccggaca gcctggatat ttttacgatc
     1081 attatgctgt tgttctatta taatctggga acagcactgg agcggacctg gggaacctac
     1141 cgttacaacg tctatatttt ttccggtatg ctgttcacga ttgccggcag cttcctgtgc
     1201 atgggagttc tttacctgct caccggaggt atggcaacgg agaccgcatc cgtggtattt
     1261 tacagcgggt cctatgcgtt cagcacctac tatatcaacc tgtccatctt tctggcgttt
     1321 gccgcaacgt atccggatat gcaggtactg ttgatgttcg tgatccctgt gaaggtaaaa
     1381 tggatgggaa ttctggatgc cattctgatg gtctatacgg taattgctgg aaatctgttc
     1441 accaagtttg cggtagtggc atcacttttg aattttgtgt tatactttta cagactgcat
     1501 cgcagccgta tctcgccgaa acagatgcac cgcaaggcgc agttcgaacg aaaatccaat
     1561 gctggacggg cgaaagtcac aagacacaaa tgtgccgtct gtggacggac agaagaggac
     1621 gatccgaatc tggagttccg tttctgctcc aaatgtaacg gcaactacga atattgccag
     1681 tatcatttat ttacccatga gcacgtaaaa tgataataag atttgcaata tccgttacgg
     1741 catgactgct tgcaggcaat gccgtgcgga tattagcaaa tcgaacagac atgagcaaga
     1801 aggacgatag tccggattgc gaatgtctgt acactttcgg gagtgcgaag cacggagaaa
     1861 gtgtattatt gcaagaggca tacggcatga ctgcttgcag gcaatgccgt gcggatatta
     1921 gcaaatcgaa cagacnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnacgga
     2041 gaaagtgtat tattgcaaga ggcatacggc atgactgctt gcaggcaatg tgcgaagcac
     2101 ggagaaagtg atgttattat aaaggagacc ctatatgaac aaagaagtat tatttgccca
     2161 gaccttagag caggtccgca agaccgccaa ggagcagggc aactgcatca gcgaagaaca
     2221 ggtaagggaa gcattcgccg agctggatct gagcaatgaa cagctgcaga tggtatttga
     2281 ctatctgtta aaacataaaa tcggtattgg acagcctatg gatcccgatg aattcctgac
     2341 ggaggaagaa aaggattact tacaggaata tctggatgaa gtagcggcat taccgaccta
     2401 cagtgacggt gaaaaactgg cattttccat tgcggccatg gcggaagaga cggatgcgca
     2461 gcagcgcctg attgaattgc atctggcgga tgtggcagag atcgcaaaac tctatgccgg
     2521 acagggtgtc ctgctggaag atctggtggg agaaggcaat ctggcgcttt ccttcggtgt
     2581 gaccatgctg ggcagtctgg agaagccgga cgaagtcgag ggaatgctcg gcaaaatgat
     2641 catggatgcc atggaagaat acatagccga acatgcagaa aattccaaga tcgacaagcg
     2701 agtggaagat aaggtcaaca aggtagcgga taaggccaga gaactggcgg aagaactgca
     2761 ccgcaaggtg accacggagg aactgatgga agagaccggt atgtcccgca agatgatcga
     2821 ggatgccata agaatgagcg gtttcaagat tgaggatatc gacaacaatg caaaagacag
     2881 tttatgagaa ttataaatac agcttgcagg atacggcaaa ggtctacata ggcgcaaaat
     2941 atacctttgc agagctgctg gatcaggacg aggtggcgtt taaattccgc ctgatcgtgg
     3001 aacgctatat tttagcggaa aaagaggtgg atccccagga tacgctggag actcatctgt
     3061 attatctgaa gccggacagt tttcttgtga agatctacga c
//
LOCUS       DAWDHD010000235         3070 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_1934_length_3070_cov_12.125041, whole genome shotgun sequence.
ACCESSION   DAWDHD010000235 DAWDHD010000000
VERSION     DAWDHD010000235.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3070)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1934_length_3070_cov_12.125041"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          806 a          843 c          647 g          774 t
ORIGIN      
        1 gcagatttac agtctgtccc ctttggccac tcgggaatcc atccatgtca ggcaagagta
       61 ccttaacaat gtgaaattgt agcatatgtt cacacaaatt gcaagatgtt tttattattt
      121 tagtgtaaaa gttcagccat aggatgacag aagcgacgat tgggtgctcg cacacaaagc
      181 gtcaattctg tcatcctatg agcagagcgc caatctatga gcaaaagtag tcacgaagtg
      241 acgaaaaagc gtgctaacgc aactttgcga atgacgcagg ctgaactgct tcaatataat
      301 ataaatagaa aggatactgc gacagttgca tcagtccgta atcggttacc tgatatccat
      361 cctgctgcaa tccggcgatg gcatctgcgc caagttcggt cggtgtaaaa taccagaacc
      421 tgtcggaatc cgcctggttg taatctccgt tcacgatttc attatccgga tagtaataat
      481 acagcacggt ccaacccaga tgttttacac cattcgtgac catatttaca tcctcatcgg
      541 gggtaccgat cagatccagt gtcttttcgg tctccacctt ctgctgttca tagctgtcat
      601 agcattcccg atagttctga attcccaggc cgaataatac taccagcaat accgccagca
      661 caattctgct tacagcctcc aggcccttcc aggacttctt ctccagctct gcagccagtt
      721 ccagcacacg gctgctgccc accgccagca tgacgattgc caccgcgctt aacggataca
      781 gatatctctg tgccagcatc ggtgccataa caacacttaa caaataagca aatgccaaag
      841 ttccaaggat tgtgcaggct cccactgcca tggcatatgt cttgtcagac cagtttctca
      901 cagaaggctt ctgcatatgt actccgtctt tttccacaga gaatacagaa gaatccactg
      961 ccagggtcac caccagaaac agaatcacca gcgggaatac gataccattc atccctctgc
     1021 cgcccataac gatctccaga ctctggtcca gtcccaggat ctctgtcatc caccagcctc
     1081 tgctgacatt tttcaagccg gcatagaaga aatatagcca gggtgcataa cctatgaaaa
     1141 atgctacgat ggccagaaca ccctttatcc atgttttccc acggtatttg atccatacgg
     1201 ccactccggt gaaaaacatc ataatgccta cagccaccag tgcataatag tgagaatacg
     1261 ctgctgccaa tgcccacagc accatgcctg cccaggtctt cctgctgccg tcagcaatga
     1321 ctctgtagga acaatagaag caacccatta cacacaggaa agccagcgca tacatgcgga
     1381 cttccagatt gtactccaga catgcctgtc ccagaccgga gatcatcaca aaaaatgttg
     1441 ccggaagcag tccgaaatgt ttccgcacaa cggtcagtgc cagtacgatc ccaatcacga
     1501 agggcaccag agaggccaga tggaataccg gtaccttata tccgaacagg gtcccgaata
     1561 atttcagcca gtaataatac agtggcggat gattgtccca gtaatacaat acctgcaaaa
     1621 tgccatccac atccttgtgg gccgtattcg cagaaaacgc ctcatccccc cacagtacgc
     1681 ggtcggacat catgctgaaa ttcagacaca ccaccagcac tgtcaatgcc agtactgcca
     1741 ccggtcctat gtatttgcga aatacgctcc atgccttgct atcggtaatc ttactcagga
     1801 cgctccggat cttctcatgc tttccggctt tcgcttcttc cacgaacaat tctgcaatgg
     1861 catagaccaa catcatgctg atctccagac atagaacttt cactgcatat tctctggatt
     1921 ccaacagctt tctcaagatc gtattgccgt cattgccggt cagcgacaac aggatccagg
     1981 gaatgaccag catacagagc aggcaggtaa gataacgaag gacatattct cccttcccga
     2041 tcgtgacaga ctgtccgctt tttttgttat tttttaaaat agattgccgc cagattccaa
     2101 tggataccag cggcaacagc agggtaataa tgatggtgaa catcgaatat cctctctgtc
     2161 ttttgtattt gtaactattt atagggcaaa gtaaccacca tgagcaaaaa ggaagcctcg
     2221 cagaggcggt tttgcgaatg gtgttctgaa tagttacata tatttattct tctacatatt
     2281 tcaggatcgt tgccgaggtc tgtggcatct cgatcagaac cttgcctgcc ctgatctcat
     2341 actccgcacc ttccatgcaa tacccgtcat ccctggactg cagaataact ttcatcttac
     2401 ctgtcttagg ggttccgatc tcccacacat gaatctcttt ggtgatggga tgattgttat
     2461 tgttgatcag gatcgcacac tgtcccttgc ggttaaaacg tccgtaagaa ataaagttgt
     2521 agtcattttc caacattttc aaggagccga tccgcagctc ctcgttctcc cggcgcaggc
     2581 ggataatgtc cttatgaaat gccagcagca cctgatcctc atgtccccag ggatacgttc
     2641 tgcggttgtc aggatctgtg aaaccacaga caccggcctc atctccgtaa tagattgtag
     2701 gagcacccgt ccatgtcatc tggatcacaa tgccttcccg gaagactgcc ttattgatgc
     2761 cctgctccgc agcctggggt cccagtgtat tggtacgtcc caccttatga ttcgttctcg
     2821 tcagaaaacg ggaatgatca tggttggaca gttcattcat agccacctgc cagctgggca
     2881 tggagaaact ggaggtatga tgccgcatgg ctccccagaa gctctctgca ttgccgagca
     2941 gatcctctct gtagtcatcg ctgtgcttct ccataccggt gaggaaccag gtcacgggct
     3001 ccatgaatgc atcgtagttc atgacagtat cccattcgtt gccctgcagc cagtctctgg
     3061 tattgccgta
//
LOCUS       DAWDHD010000236         3067 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_1940_length_3067_cov_9.420319, whole genome shotgun sequence.
ACCESSION   DAWDHD010000236 DAWDHD010000000
VERSION     DAWDHD010000236.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3067)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1940_length_3067_cov_9.420319"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          706 a          847 c          672 g          842 t
ORIGIN      
        1 gttttctctt catggtatcc acctgacgca ctctggcaaa atgctccggt gcgaaggtcg
       61 gaataccttc atactggaac ttctccctgg gcatgcacag ggtatctccg atggagaaaa
      121 tgccgggatc gaagacaccg ataatatcac ccgcataagc ctcgtttaag atcttacgtt
      181 catccgccat gatctgctgg ggctgggata atctcataac cttattgccc tgcacatgac
      241 gcacttccat gttggcatcg aatttaccgg agcagatacg catgaatgcg atacggtctc
      301 tgtgagcctt gttcatgtta gcttggattt tgaaaacaaa ggcactgaac tcatgctccg
      361 tcgagttgat catgccgatg tctgcttttc ttggcagcgg cgtggtagtc atattcagga
      421 aatgctttaa gaagatctcc acaccgaagt tggtcaaagc agatccgaag cataccggag
      481 tcaggttacc cttctgcact tcctccagat caaactctgc gctggcaccg tccaacagtt
      541 ccacctcgcc aagcagcttg tccgctgcat cctctccgat cagttccttc agatgttctt
      601 cgtcctgaat ggggatctcg gtggaagttc cttccttcgt tcccttctcc gtatcggaat
      661 atgcgatcac acactttttc tcacggtcgt agacaccctg gaattcctta ccggaaccaa
      721 tgggccagtt aatggggcag gtggcaatgc caagctcctt ctcgatatca tccagcagtt
      781 caaaggtatc tcttgcctca cggtccatct tgttgatgaa ggtgaaaata gggatcttgc
      841 gcatcacgca gaccttgaac agctttctcg tctgggcctc gacacccttg gaagcatcga
      901 tgaccataac tgcggagtcc gctgccatca gggtacggta ggtatcctca gagaaatcct
      961 gatgtcccgg ggtatccaga atattgatgc agtaaccgtc atattcaaat tgcaatacgg
     1021 aagaggtaac ggaaatacct ctgtctttct caatctgcat ccagtcggat accgcatgtc
     1081 tggccgttgc cttacccttt acgcttcctg ccaggttgat ggctcctccg tagagcagga
     1141 acttctcggt cagggtcgtc ttgccggcat ccgggtggga aataatggca aacgtccggc
     1201 ggcggttgat ctcttctgta taattattac tcactactat agtcctccaa atatatctct
     1261 tatatctcta aaaatctatt ttcgcagttc ttcgggatct gctatctatg atggatctgc
     1321 ctttaaaaca gagcttcctt caaactccgg ttggattccg aaataatcca ataccgtagc
     1381 tccgatatcg gaaaaagtcg gtctggttcc gcgattcccc ggtgtcacag gcgcaccgta
     1441 cataagaaac ggagtatatt ctctggtgtg atccgtggtg gctgtgtaag cgggatcaca
     1501 gccgtggtct gcggtgatca tcagaatatc atccggcttc atgtgtgcct ggatttccgg
     1561 cagcttttca tcaaaataag ccagtgcctt cgcatagccg tccacatctc ttctatggcc
     1621 gtagagcatg tcgtaatcca ccagattgat aaagcagagt ccttcgaaat ccttgtccag
     1681 atattccagt gtccgctcaa tgccttccgc attgccggaa gtgtatacat gctcggtaat
     1741 gcccctgcct acgaaaatat cctggatctt acccacagca atgacatcct taccggcggc
     1801 cttcagctga tccagcatag tcactgcggg aggcagcagg gaaaaatcgt gtcttctggg
     1861 agtacgggta taatgtccgc tctcaccgat aaaaggtctg gcaatgacac ggcccacgcc
     1921 gtgctctccc tgtaagatct gtctggcgat acgacaataa tcatataact gctccacagg
     1981 aaccacatcc tcatgggcag cgatctgaaa cacactatct gcggaagtat atacgatcag
     2041 tttgccggtg cgcacatgct cgtccccgta atccgcaatg acctgtgtgc cggagtacgg
     2101 cttattgcac agaacatctc tgccggtacg gatccggaac tcttccagca catcctcggg
     2161 gaaaccgtcc ggataagtgg gcagcggctt gggagaatag atacctgcga tctcccagtg
     2221 tccgatggtc gtatcctttc cacgggaacg ctccgtcatg cgggtataag ctgccgtggg
     2281 atggtctgtt ttctctccca cttccacgcc gtcaatatta aacaaaccca tgtttttcat
     2341 attttccata tgaaaataag ggctctttga cacgctcccc agtgtattta cattgatgtc
     2401 accgtaagct gcggcatccg gcatctgtcc gataccgaag ctgtccaata caattaaaaa
     2461 gattctcttc attgtttctt ccttttgtca agttttccag gttaagtcat gataccatcc
     2521 agattgttcc gtgctccgca ctctcacaat ctggaccgca ttcgtaatcc gctcattttt
     2581 ccgcgaaaaa tggctcctta ctcctccgag ttcttatgat accatccaga ttgcttcgtg
     2641 cttcgcactc tctcaatctg gaccgcattc gtaatccgct cattttcttc gaaaatggct
     2701 gcttgctcat gtggtacggg ttacgaattc aaaaagcctc ctgcaagcaa gcttgcgtag
     2761 gctttttaat tgtaaccctt ggtatcataa cataattttt cccctatgta cacaaaaata
     2821 tcactgtgca atggtgctga tgacgatatt ttccgccgca atatccgtct ttctttttac
     2881 gatatcttcg atctgtgccc gctgcacatc cgtcagttct gcggcattga ccaccacatc
     2941 cacggcatcc ccattgatgc tgaccacagc ttcactgaaa cctttcgcct gcaaaagcac
     3001 ctcggccgcc gcatcctttt ccgcgacatc agtcatgcgc accatgctgg ccaccgcttc
     3061 ctgtttc
//
LOCUS       DAWDHD010000237         3058 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_1945_length_3058_cov_10.745921, whole genome shotgun sequence.
ACCESSION   DAWDHD010000237 DAWDHD010000000
VERSION     DAWDHD010000237.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3058)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3058)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3058
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1945_length_3058_cov_10.745921"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          752 a          673 c          845 g          788 t
ORIGIN      
        1 tcatcaatgc ggggatctcg ctggtggtgc agacggtggc agcagtgctg cttcttatgt
       61 ttacgagcct ggatctgtac agcattgcca ttgccaatac gctgtattcc ggtatgatgt
      121 gcgtactgaa ccagtgggca gtaagaagag cagtaggcta ccggcaggag attgtcagaa
      181 cttttgtaat tccggcgttc gcatccgcgt ttatgggagc ggcagcctgg gcaatctacg
      241 aaggtctgta tatggtgacg aaatctatgc ggatctctgt cattccggcg attgtgatcg
      301 ctgccgtagt ctattttgtc atgctgatac tgatgcgggg catcacggag caggagcttc
      361 gcagcttccc gaagggatat ctgttagtga aggtagcgaa gaagtgccgc ttattgagat
      421 aatgagaagc gatgacagct acgatgctct aggtgaagaa acagtgaaga gaataagtac
      481 agaaaggagt ctgcctcatg acaatgttat tgtcggaggc agatttttgc gtacatgggg
      541 cgtagatata aggagcatgc tgattaagcc ccgggaagat cgagaaagcg gggcgcagag
      601 aactaaagaa gctgattaag ccccggggag atcgagagag cggggcgcag agaggaaaag
      661 aagctgatta agccccggga agatcgagag agcggggcgc agagaggaaa agaagctgat
      721 taagcccccg gggcatgaaa ccacgggggc acaaggtcaa ggtatatcat ggaatagaaa
      781 tgaaaaactt tcaaatgtat caattatctc tggcagaacg gatccccacc attcttgaat
      841 aatgtgatca tttcttgacc gacactgata gtggagggca gatccgggac atcctctctg
      901 gagaaccagg cagcctcgcc aagttcggat tcctgcaaag taatagaagg atccccctcc
      961 agcttcgcga agaagccgat catggcggaa tcgctgaagg accagggctg ggatttgtaa
     1021 tagaccagat cgctgacctt taaaccgact tcttccatga tttcccggcg ggcagcaccc
     1081 ttgaaggtct caccgacttc cacgaagccg gccaccagtg cccagtgacg ataggaaccg
     1141 ccggcgtagc gggtcagcag gatcttgtcc tgttcggtat ccacgatggc gataatgacg
     1201 gctggagaga tcttgggata ttcgatctga ccgcactccg ggcagatgca ggcccgctct
     1261 gtggtgctgt gaattgtggg atgtccgcag cggccgcaga atttccggtc cagacggaaa
     1321 cgattgatct gggtggcagt gattccggca aatgccagat attccggctc aaaattacgg
     1381 aacacaccgg aagcgtagag gtcttcttcc gggagcatga gagcaccggc atcctccgtc
     1441 gtcttgcgga agaatgctcg gtcatcaatg gaaaagagat attcacagtc cagaaaaaaa
     1501 cgttccatag gctgacggat tcccaagagg ccatggactt cttcaaaagt aggaagatat
     1561 acttgaggat gctccgagat gccggcatcg gcggaagtat ccgttacatc actgttttct
     1621 gtataggagg gcagatagac cttttctccg tcaaataaca gcagataatc ggaaggttta
     1681 ggatcctggc ttcggaattc cgggtaatat ttatgcggtt cgatcttatg gatcatcata
     1741 gagtatttct ccttttatgg ctatttttca gaaagtgttc ataagaatat agtagtccac
     1801 cgtggggaaa ctgtcaaacg taacagttca gccgcgccgt tcacgggagt cgcgttaaca
     1861 cgcttttatg tcacttcgtg actacctttg ctcatagatt gacgcttagc gcctgcacat
     1921 atttgcccgc aaaatcatgc aagcatgatt tctgcgccca gatatgttca gaactgaact
     1981 gttgtggaaa ttatttgaga ttccgggata tttgtgttaa aatattttgt gactgttctg
     2041 aggtgttcat ttctcgaatg cccctgtgaa cagtcacgga atttatcaga aaatacagca
     2101 tgagagtaca aaagtgagta tctcatgtgg aaacgaggga tagtatgtta cagatgatct
     2161 tgccggaggg aagcagttct ctccttgctt tttttggaat cctttttgct tttggagcga
     2221 cagtactggc gacggcaaaa ttaagcccgt atctgccgaa ggacgccgga agagaatttg
     2281 cccatgacgg aaaattgtct gcggggaaac ccagaggtgc cggtattatt tttgtcatgg
     2341 catttgtggc atccgttctg ctgttttccc ctttaagcgc agagctggtg atctatctgc
     2401 tgctgatcgt ggtatgcatg atgacgggat ttctggacga tgcttccaag actccctggg
     2461 gtgagtacaa aaaaggtttc ttagatctgt gcgtggcggc actggtagcc atcacgttcc
     2521 tgaaatataa ttccaacgtg gtggagctgg cactgtttca tgtaaagttc accctgccgc
     2581 ctgtggtatt cgccattctc acggtgatcc tggtatggac atctgtgaat gtgaccaact
     2641 gctccgacgg tgtggacgga ctttccggaa ccctgtcgat cattacgatc atgtccatct
     2701 atatcgtgga ccgtatgaag gatgtgaatg agacttactc tttcctgatc ctgctgtttg
     2761 ccgtatgtat tctgggctat ttgtggtaca atgccacacc cagtaagctg atgatggggg
     2821 atgcgggttc ccgggctatg ggattgttca tcagcatcgc tattttgaag agcggctgcc
     2881 cgttcctgtt catcccgctg gcactggtgc tgatcctgga cggaggtttg ggactgctta
     2941 aggtttccct gctccgcttc ctgaagattc atattttgaa aaatgtgcgt actcccttgc
     3001 atgatcatgt gcgtaaggtg tgggactggt ccaatacaca gaccgtattc cgttttgc
//
LOCUS       DAWDHD010000238         3038 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_1970_length_3038_cov_10.568220, whole genome shotgun sequence.
ACCESSION   DAWDHD010000238 DAWDHD010000000
VERSION     DAWDHD010000238.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3038)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1970_length_3038_cov_10.568220"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          796 a          666 c          770 g          806 t
ORIGIN      
        1 ctgcagtggc atccggtccc ctgatcacca cggtaaacga tctggtggca gtggtaacct
       61 attatggaat ggcatggtta cttttgatcc ggtaataaca gagatgagtt ttcatggtgg
      121 ccatgcttta atcgcatggc cgctttttag attgcgcaaa cagtttcagg aagctgtgct
      181 ttcggaagca tccggtgtcg tctcagggat aagattgctg taataagcgg tctttccgga
      241 taacacgtca tcatagaatc cttgtttcca gtcgatgtag tccacggcct cctgcagctg
      301 ctgcatggag gttaacagtg cttctttttt ccgggagagg atgtcttttc tggcgggaat
      361 ggaggattcg ccctccagac ataaatgcag atattctttc atttcggcga tggagagacc
      421 acagtttctg aggcacccga gactctggat ccatttgata tcgtggtcat cgaagacacg
      481 ataattgcgg ctgtcccgct tcacgttggg taccagtccc tccttgcagt aaaattttaa
      541 gttctcatag gtcatgccgg tcagggcaca ggcttctttc atagaataca taaaatggct
      601 tcctttttgc tcatgtggtt acttcgccat ataaaactgc ttatctgact gaatgcaagc
      661 attcgcagaa cgcagttttg catggctctg aaaagttaca aaaaagtatt gccggtagtt
      721 actaccgttt gataaaatca taacgtaaaa tagacgcact gtcaatgcgg aaaggattac
      781 aacacttatg aatcattcag gaattttgaa aaataagctg ttcttgtatc tcacagagtt
      841 cttcgccggg atgtccgtca tggcggtgga actgggagcc agcagactgt tggcaccgta
      901 tttcagttct tcccagatcg tatggaccat tgtcatcggc accatcatga tcgccatggc
      961 gctgggcaat atctacggcg gtaaaagtgc ggacaagtcc ccgaacccgg acaaactcta
     1021 cggcaggatc ctgatcgcgg ccatctggat tgccctgatc cctgtggtgg gcaagtacat
     1081 tatccttggg atctccgcac tgctgatctt taccgtcagc aacaatttcc tcatcattgc
     1141 ggcgtttgca gcctgcatgg tgatcttcgt gttcccctta tttttactgg gaactgtgac
     1201 cccttctctg gtaaaatatt ccgtggatag tctggatgac agcggacaga ccgtgggaac
     1261 tttaggtgca ttcaataccg tgggaagcat catcggaacc ttcgtaccga ctttcgtgac
     1321 gatcccggcg gtgggaacct ccattacatt tctgatcttt tccggaattc tgatcctgct
     1381 gtctgtgatt tactttatct gccagcatac gggaaagaaa aagattgcag catcggtact
     1441 gatctttatt ttatgctgtg gtctgggata ttcggattcc tttgctttct ggcagaagga
     1501 tctgacctac gaaggagagt ccatctataa ttatctgcag gtatcggaga cggacagaca
     1561 ggtggtcctg tccaccaacg tgctgttcgg tgtccaatcc ctctatatga aagaaggggg
     1621 acttaccggc atgtattatg actatgccat ggcggcgccc cttatggtgc cggataaacc
     1681 ggtagaggac atggatgtac tgatccttgg tatgggaacc ggaacctatg cgacccagtg
     1741 ccggaaatat ttcggcgata tgaatatcga gggcgtggag atcgacgaaa agatcacgga
     1801 gctgtcgaga gaatattttt ccctgccgga ggatgtgaag gtgactacct atgacggcag
     1861 ggcttttttg caggcggtgg ataccaccta tgatgtcatc atggtggatg cctatcagga
     1921 catcaccatt cctttccaga tgtcttccgt ggaattcttt accatggtca aagatcatct
     1981 gaaggacggt ggtgtcatgg tagtcaatat gaacatgcac ggcaacaagg aaggggatat
     2041 caatcagtac cttgccgaca ccattgccaa cgtatttgac aatgtctata ctgtagacgt
     2101 ggcaggctcc accaaccgcg aactgtttgc ttcccagcac agtgatatga tagaagtatt
     2161 atccgatcat gtggagaatc tgtcagatgc gggactgcag aacatgatgc agcgggtagc
     2221 ggacaacagc agtgcctacg tgaccggaga ttacctcatg accgatgaca aggcgcctgt
     2281 agaacttctt ggcatgcagg tcatcgatca gctgattcag gaagaagtag cgtattacaa
     2341 ggatatttat gaggaacagg ggatcagcgg attgatggaa cacctgtaaa tcggcagatt
     2401 tgcaaataga gtttgaaata acataaaggg taagaatacg acggtggagt caacaggctt
     2461 caccgttttt tcctaaaaaa atataaaaac cattgaccgg tagctactac cgactgctac
     2521 aatcacgcta tatgatgcaa taatgttggt cgtaggaaga aagagacctt ttcaatagaa
     2581 ggaggtaaca gaaatgatgt acaatcccca gttggatacc ttcatttgtg tggtggaagc
     2641 aggcagtttc agcaaggcag cggacaaact gtatatttcc ccaccggcgg tgatcaagca
     2701 gatcaattcg ttggaaaata atcttggagt acagcttttt gccagaaccc acagaggtct
     2761 tgtggtgacg gcggcgggag aatccctcta tcaggatgca aaatatatgg taaattacag
     2821 taagtatgag atcactccgg tagagcccta taccagtgat gacctgaact ggactaacag
     2881 ctccagccgg gtatccagag agcatgatga tgagagcctt cgggatattg cactgacaga
     2941 gattactccc gcagactggg attcttatga taccgttttg atcggtgctt gagttataat
     3001 tgggacaagt tagaagaaac ccagtaaaat caaggggt
//
LOCUS       DAWDHD010000239         3022 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_1981_length_3022_cov_10.718234, whole genome shotgun sequence.
ACCESSION   DAWDHD010000239 DAWDHD010000000
VERSION     DAWDHD010000239.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3022)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1981_length_3022_cov_10.718234"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          695 a          799 c          666 g          862 t
ORIGIN      
        1 gccggcgtgt cggaatggca gacgaggcag actcaaaatc tgttggtggc aacaccgtat
       61 gggttcaagt cccattgccg gcagttagtg cagaatcaaa tcggttctgc gctttctttt
      121 tgtattttta taaccacatc gcatttcctg ctttttctaa tttttttcca atcatctcct
      181 atggattaat tgttacaaaa ttgttacaat tcatttgtgc ttagctaaca cagacgtaac
      241 cgaacaccag gcaacaggaa aaggaggaag aaagtatgtg tttcaaattc attaactgtt
      301 ctcagtggtt gaatgcattc tgcggctact tcaattttgg ctgctaatat cgttttcgac
      361 gaatagagcg ttccatagac gcacgaaaag gctctcccgt ttgggatgag ccttttctta
      421 tttctacact ttttctatga atttatacat tttaccgata gtcataacgg agggtgacat
      481 cattgccctg tacctgtact tccgcacagc ttccggtgac aatcggcatc atgggtgcca
      541 gatgtcccag atccacatcc atgatcacgg gaacattctt ctctcccgcc acggccagaa
      601 ctgcctggta ctgatccagt cccatcatct cctgtccgaa acacaacggt cttccgatga
      661 gaaaaccttt gacatactga aaccatccgg cttcctccat ctgccacatg gctctgcgga
      721 tggcaaaaac attcaggtca caggcttcca ggaaccagat gattccgtcc tctttatact
      781 tttccagaaa atcctgtgtt ttatcaaacc gggttcccag taaattcacc agacaatcca
      841 tacagccgcc cagcaaccga cctgaaaagc ggatctccgg ttccttttct gctgtctgtc
      901 tgccaagata ctttttcagc actctggatt ccgtggtgtt atagggcaac agaggctgtt
      961 cttcattttt cagagactcc ttttcccact tgtcatatcc atgcacctct ttggtctctc
     1021 cggtcaatat tccgaaggca tctttcaggg atgcatgcca gggctccatg ccaaaggcgg
     1081 ccgcgcaggg gccgtagacg gaggcggtat cacagatggt tgccagcaga taggtcatgt
     1141 tggtattgtc agaatacccc agataccact tgggctctgc tgcctgcaga cgctcaaaat
     1201 ccacatggct catggtctcg cacatcagtt ctccgccacc gcaggagatc agacagtcat
     1261 tttccgggct gcaatagtaa tccgtcagct cctgcccgca tttttcgggg gtgttactga
     1321 taccgatgcc ctctccggca taacagttgg ggcctaattg taactgatag cccatttccc
     1381 ggaatttctt ctgggcattg ccaaaggcag tgtaataagg ttctatctga cagccaaaag
     1441 aaggagccac aaatccaatg gtccctcccc gctgtatatg tttcggatat ttcattgctt
     1501 cgtatcctcc tatatcgccc attcgcattc cgggctatcg cccttcttgc tcatgttcgt
     1561 tcacttccct cataggaggt ttgctttgcc tgcaagcagt cacgccaacc tcctatgggt
     1621 actcccattc gcattccggg ctatcgccct tcttgctcat gttcgttcac ttccctcata
     1681 ggaggtttgc tttgcctgca agcagtcacg ccaacctcct atgggtaagt gttatgcgtc
     1741 aaaattaatc ttattataaa tatcgaaaca gtcaaaggtt gcaaaataat ccttcggtgt
     1801 ctcggcacgg cggatcagtt ccacgctgcc gtcctccttc agcagaagct ctgcggattt
     1861 cagcttaccg ttgtaattgt aacccatggc aaaaccgtgt gcaccggtat cgtggatggc
     1921 aatatagtca ccgatatcga tctttggcag catccggtcg atggcgaatt tgtcgttgtt
     1981 ctcgcacagg gaaccggtca catcgtactt gtggtcgcag ggctcgtttt ccttgcccag
     2041 tacagtgatg tgatggtaag cgccgtacat ggcgggacgc atcaggttca cggcacaggc
     2101 atccacaccg atgtattcct tatgagtatg cttctcatgg atgacctggg tcaccagatg
     2161 tccgtaaggt gccatcatat aacgaccaag ctctgtgtag attgccacat cacccatgcc
     2221 tgccggtacc agaacttcct catatacctt acgcacgcct tcgccgatga ctctgatgtc
     2281 gttgggttcc tgatccggac ggtaaggaat accgatacca ccggacagat tgatgaaggt
     2341 aatgtgtgcg ccggtctcct ctttcagctt tactgccagt tcgaacagtt ctcttgccag
     2401 agtgggataa tagtcattcg ttaccgtgtt actggccaga aaagaatgga taccgaaatt
     2461 ctccacaccc tttgccttca ggatcttata tccctcaaac atctgctccg tggtgaaacc
     2521 gtatttagca tcgcccggat tgtccatgat gttattgctg atggaaaaat atccgccggg
     2581 attgtaacgg cagctcaggg tcttgggcag ataaccgatg gtcttctcca ggaaatcgat
     2641 atgggtgatg tcatccagat tgatggtggc accgattttt gccgcatatt cgaattcatg
     2701 ggccggggta tcgttggagg agaacataat gtcctctccc tttaccccca tagcattgct
     2761 gagcatcagc tctgtcagag aggagcagtc acatccacag ccgtaatctc ttaagatgtt
     2821 gatcagaaaa gggttgggag tcgcctttac cgcaaagaac tccttgtagc ccttgttcca
     2881 cgcaaaagct tccttcagcg ccttcgcatt ctcccggatt cccttttcat catagatatg
     2941 aaacggtgtg ggatattttt ctgcgatctt ttcgatcatt tctttggtaa caaatgcctt
     3001 tttttccata agtatctcct ct
//
LOCUS       DAWDHD010000240         3017 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_1989_length_3017_cov_9.095881, whole genome shotgun sequence.
ACCESSION   DAWDHD010000240 DAWDHD010000000
VERSION     DAWDHD010000240.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1989_length_3017_cov_9.095881"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          761 a          770 c          734 g          752 t
ORIGIN      
        1 cgcggcgttc gccgcatctc ggcataagta aagacagcga atcgtgcgca agcgcccatc
       61 gctgtcttca ctatgcttat gcgcggctca ttgctcgcgt aactttccat ttactttatt
      121 tcctccgcaa ccaattccgc ccacgccaca gcaaatccag ctctgtgggc gacccgttgg
      181 gaggcaatat gtgacatgct tgtgccgtaa tagggaacgg caccttgagc gatgatatcc
      241 tgcgccagaa gtcttaccag agtagaaccc acatgcattc cgacagcatg ggaatcaaca
      301 ttgataccaa tctgccagaa cagaggactg tcggcactgg caccggccat gccaaggatc
      361 tgtccgtctt ttgctgcggc aatacccaga acatccgggg catagggatc gaaggcaaat
      421 gcctcatcaa agcggttgtc ttcccggaac tgcagaatgg cattttggtc ataccgaatc
      481 aaatcgaagg cgagagcatc agacgcatct ggcaaaggaa tgccagagga aggcatgaca
      541 tcggcttccg gcagaaaaaa aggatgcgcc tgcgcaacac ggaggtggaa cggcaacagc
      601 agttcttcta tttcccgaag tcttttcgca tcgaagaacc attcaccggt tacatcggca
      661 taacgctttc tacattctgc aacaatttct tcggaaccgg taaataataa tttcccattg
      721 acgacagcaa ttttcaagcc acggatcaaa gcctcctgaa agcggcggcg tccttcctgt
      781 ggagcataaa ttgaaaaatg gttctccgaa tcagcaacat ctgcgggact acagcagtaa
      841 tccagtgcca gttgacgaga caattcttct ccatacataa atgtcggaac tcctttcaca
      901 atggaccgca tcggacggtt tgaatgctcg aaaaagcatt tcacgtaaag gttcagaatg
      961 tctgtggctc ccaacccttc atcacccgtt ccacggtata ctccgcagct tccgcatccg
     1021 tcagttggtg ggagaagtcg gcgcgggtgg caggggaggc accggggccg taggagttgt
     1081 attctccgta gtagaagtgg tcgtggggct ttttccagtc ctgccagccg gcggggtgga
     1141 tatgtgcacc cagttcacag tgcaggaaca ctgtctttgc ccattctctc cagggacgtc
     1201 ccagatagat ggaattgggc gggcagtcgc tctctaattt gcattcatgg aacacgtagc
     1261 cgtagggctc ttcctgcggg gtggaggcag cagtcacata gccgtagatt acttcagact
     1321 ccggactttc tggtggaatc cggtctccgg gcagcttgga gaagatcgtg caatgctcaa
     1381 accaggcgat tccactgccg aagatgaaat ccacatcacc ctggataaag caattttcat
     1441 aataatggcg tcctatggtc cggggcttga attctccggg acccttgaat ccgccgggct
     1501 gagcctcttt taatggcagg ggtgcatcga agatcgtatc ctgactgcct aagaaccggc
     1561 agttataaaa ggcattgcgg tcaccgtcca catagacggc cagtgcctgc cctacggtgt
     1621 ggccgtagcc ggaatcgttc tggaaagtca ggtttctggc ggtaaagtca tgagtgtcga
     1681 tccggacgga agcagtacgg aaggtacctc gcttatcccc ctccggcatg agatcgaagg
     1741 cagcatctcc atagacaagg atcgtctcgt caccgccggt accctcaaaa gtaatattcg
     1801 gacgggtgat taccagcttt tcccgataaa ttcctttacc gatatgcagg atcaccggcg
     1861 gcagctctga aacgggagcc gggaactgct ttccggattc tgaggcagga acatccggca
     1921 gggaagccaa tgcttctccg atggtggcaa aagaagtgcc cggaattgta tttccggaca
     1981 tatttacata caaatgaatt gtgtctgaca taaatagaat acctatacct ttccgtacct
     2041 ccgcattttc aagcccattt ctccgctttg cttcgatccg tgctaaacat gtgccactgg
     2101 cacataatat atgctttagt gctttgtatt atagtacaat gtgaggtaca aaattgtgcg
     2161 taaaaaataa ttttcaaatg ataacttaca acagcttttc aattttcaga tccggctggt
     2221 ctgccagatt gatatattcc tcgccgtact gtactacagg gcgggacaat ttttccttat
     2281 tgatgtaaat gattgtgact tttcgtccat cgttcaacag acaacggttt tgcaggcagg
     2341 aatttaccac gttttccagg aaaggaagca gaatggaaac atcgtatttc tggaagcctt
     2401 ccgtttcgaa catttcaatc acacagaacg gacacaaagg tcctctgtaa cagcgggctg
     2461 ccgtcatggc atcgtagaca tccgcaatgg ctaccatccg tgcataagga tcgatctggt
     2521 tcccttttaa tttcgagggg taaccgctgc cgtcactacg ttcgtggtgc atcagagctg
     2581 catttttcac atgcacgtcc acatcctgag actgcagtaa ttcatatccc agagcaggat
     2641 gtttttttac cgtggcaaac tcttcctcgg acagtctgcc gggcttggcg atgatggaat
     2701 agggaatcag cattttgccg atgtcatgga acagaccgca ggcagtagcc agttccacgt
     2761 cttctttcgg aagcttcagc catcccgcca gaatattaca gatcagtgcc acattcagac
     2821 tgtgggtata ggtggaatca tcgtattccc gcatattttg aagcatatcc agaatactga
     2881 tgctgccgga atgattcgaa acaatggaaa gtgtgttttt cagcagagtt ttcacatcca
     2941 gggctgtcat cttctgcacg accagattca tattttcccg gaaaagatcg acttcattct
     3001 caaaatcttc tttaaac
//
LOCUS       DAWDHD010000241         2976 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_2021_length_2976_cov_9.177679, whole genome shotgun sequence.
ACCESSION   DAWDHD010000241 DAWDHD010000000
VERSION     DAWDHD010000241.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2976)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2976
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2021_length_2976_cov_9.177679"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          680 a          823 c          599 g          874 t
ORIGIN      
        1 gtatagccct tttcctttgc atccgccacc agtttgtcga tgaattcctt caccttcggg
       61 taagtggcaa aatactgttc aatgtactgt gccgcttcct ttttgctgat actcagatcc
      121 tgactcagtc caaaggaact gataccgtag acgataccga aattgactgc cttggcattc
      181 cggcgctgca gatccgtgac ttgctcgaaa ggagtatgga ataccttgga tgccgtgatc
      241 cggtgaatat cctcttcctg tctgtaagca tcaatcagct gttcgtctcc ggacatatga
      301 gccagcacac gcagttcgat ctgggaatag tccgcatcca tgaattcata gccttctctg
      361 gggataaata ccttgcggat ctgtctgccc agttccatac gcatgggaat gttctgcaga
      421 ttcggctctg tagagctgat acgccccgta gcagtaatgg tctgattgaa attcgtatgg
      481 atcctgccgt cctcttcaat aaaagctgcc agtccgtctg cataggtgga ctttaacttg
      541 gtcagacctc tgtattccag gatatctctt acaatagggt aatccgccga cagcttttcc
      601 agcacatccg cagctgtgga atatccggtc ttggtcttct tgccgccggg gatctgcatg
      661 gtctcaaaca gtacctctcc cagctgcttg ggggaattga tattgaactc tgttcctgcc
      721 tgtgtgtgaa tggactgttc cagttcctca atgcgggcca ccagtgcatc cccataagcc
      781 ttcagttcct cccgctttac agccacacct tctttctgca tgtcatacag taccagtgaa
      841 agaggcatct ccatctcctc catgagacat ttcatgcctg ctgcctccag cttgtccttc
      901 aggacttttt tgcctttccg ggagacatag gctccgtagc acgcatactc catggctttc
      961 ccggcgtcct cggaaagcag tgtccggaaa tctctcttgc cgaatacttc cgctctgccc
     1021 gggatcagaa ttcccagatg ctccgaagca atggcctcca tgtcataatc attcttcaga
     1081 ggattcagca gatacgccgc gatcaatacg tcaaaatact gttctgtact gctgcggtcc
     1141 agatagtcat actggtgctt cacatcaaag gtagcgatct gtgtatccgt acctgcggaa
     1201 agttcctgta gagcttccgt gatctctgcg gtgtcatcca cagattccac cgcttccgca
     1261 aacagactta actgcccttc cggttccttt tcatgggtca gggcataaaa taccgtctcc
     1321 tcatcggaca cagacaatgc cacccccagc aattcttctt ttttcgtagt agcatccact
     1381 ggttccgtga tcagccacag gccgatctcc ttctgtttct gcgctttttt cagatattgt
     1441 aaaaattctt ttttatcttc aatctgtcgg aagctttctg cgatcttgct gtcattggcg
     1501 gcggcatctt tttcaaatct tcccaacaaa tttttgaatt ccagacgttt gcacaaggta
     1561 taggcttccg gggtatagaa cccctctgct ctggcctgtt cgtaatccaa ctgcaattcg
     1621 cagtcggtct tgatcgttgc cagcgtcttg ctcagatctg ccagatcctt atgctcgatc
     1681 agggattccc ggatactttt tttcgagatt tcttccacat gggcataaat attatccaga
     1741 gaaccgtact ccaccatcag tgcctgtgcg gttttctctc ccaccttcgg cactcctgga
     1801 atattatcgg cggtatctcc catcagggct ttcagttcga taaactgtaa cggagtcacc
     1861 tgataggcag ccagtacatc tgcggcataa taatcctcga tctccgttct gccctgcttc
     1921 gtcttcggaa tccggatctt gatatgatcc gtggcaatct gcagcagatc tctgtcaccg
     1981 gagaccagtg atacttccaa tccggcctgc tctgcttttt tggccagcgt tccaagaatg
     2041 tcatctgctt ccagtcccgc ctgctccata atggtaatat gcatggcctt caggatttcc
     2101 ttcatgacgg gcacctgctc ccgcaattcc tccggcatag gctttctcgt tcccttgtaa
     2161 gcctcataca ttttgtggcg gaaagtcggt gcatgaacat caaaagccac tgccagatat
     2221 tccggctttt cctcctccag aattttaaac ataatattca aaaaaccata aatggcattt
     2281 gtgtgcagtc cctctgcatt actcagatcc ggcactccgt agaatgcccg gttcaatata
     2341 ctgtgtccgt cgatcaatac cagtttctga ctcatactct gtctatccta tcttaattct
     2401 gtcctcttga ttctatcagg tgtcgctaag agatgatcca tcccagaaat cccataagaa
     2461 aaatcaccgt cagcacttcc agtacggccc ctgtcttcag aaaagccata tgaaatctta
     2521 ccttgcgttt tgcttccgca agtctcagtt ccagttctct ctgcaggtca aaagcatcgc
     2581 cgtcttccag acgctgcata ccttccgtta caaggaactg aatcaccaat tcctccttgc
     2641 agcccgggca ttcccgcata tgctccccga attcctgcaa ggtctgaaaa tccagttttc
     2701 ctgcaatgaa atcaggaata ttcttttcaa attctttgca attcatgctt ccaccctatt
     2761 ccaaaacagt tctatagatt gcgttcccat atactcatac acttctacta tacaacatct
     2821 tgtgtaaaaa gactagaaaa atagtagcaa gattccagaa aaaaactctt gtcaactttt
     2881 ttaatctatg ataagataat ctctgttgtg agtgtttgaa tgccggcgtg tcggaatggc
     2941 agacgaggca gactcaaaat ctgttggtgg caacac
//
LOCUS       DAWDHD010000242         2971 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_2027_length_2971_cov_17.232853, whole genome shotgun sequence.
ACCESSION   DAWDHD010000242 DAWDHD010000000
VERSION     DAWDHD010000242.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2027_length_2971_cov_17.232853"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          789 a          789 c          614 g          779 t
ORIGIN      
        1 gctccgtcca tgtcaggtat ttttcaatca ttttcccggg aaacatcttg tacagcaccg
       61 gtcttcccag acttcttgct attccaaaag caatacagga acccacactg attcctatat
      121 aattatagaa aaatcccggc accactccaa agagcagcac tcctgccaga cagctgatgc
      181 cacccggtat gatgggaaac accacctgaa cgatctggat cagcacgaag atcagtgcac
      241 cgatcattcc gaagcggttc acaaactgct gcatggtctc caccgattgg aaaatgccgc
      301 tctgatagcc ccagactgcc agtattccac agaggatcag actaacccag gatgcaatat
      361 tgatggccct ttgccaggtg tttttctctt tcgacctgtt tttcttcgat ctgtcgctgg
      421 tatagttgct ccatacttcc cgcaaatctt ttttgggaaa atatttctgc ggactgtttt
      481 gcataactgc acattcctct gcgttcatcg tcttctttct ccttccagtt cttcaggtct
      541 ttcaagaatt cctcttcggt ccggtactgc cagccgtttt ttccctcttc cacaaccgcc
      601 cgcaggcatt gatcccttct gcataataac ggtaatcctg ctgccagtgc ttccgcatag
      661 gtaagcccct gggtctcact ggtggaagcg ctgacgaaca gatctcctgc cggataataa
      721 gatgctactt cagcgggtga taccattccg gtaaaaataa cgcttcccgt cacacccagc
      781 tctgctgcct ttttccgtaa agtctccaaa taaggaccgc cgcctacaat catcagtatt
      841 gtgccgctct cctgaacttt ctgctggtac tccagcaatt cttccacatt cttctctttt
      901 gccagacgac ctacatataa aagcactgtg gtcttccggt ccattttata ttttcttcgg
      961 attctctccc gggaatccgt ccgggtctgc gcgtcatatt tactcagatc aatccccgat
     1021 gggatcactg ataccggaca atgaatgcca tagtctttta gtaatgtctc tatttttgtg
     1081 ctgggagcga tcatactgtc caccttctcg gagatctgtc gcgtcaggaa ctgtaccata
     1141 tccctgcccc atttcttgta aggggaaaaa tagtgggtat aatcttcgta tacagtatgg
     1201 taagtatgca ccagagggat cttacattcc tctgcgatct tttttgccat gaaaaaggta
     1261 gaaaattcac actgggaatg tacaatatcc ggattccact ctataatccc gcgatacagt
     1321 tcgcgtcccg gggacacccg aagtcttgct cccggataaa tatatccggc attcacggaa
     1381 cccatataga tcacaccctc ttcttcgtag gaacgagctg tacgggacaa ggtcaggatc
     1441 ttcacttcat gtcctctttg ctgtaattcc tcccgcagat tgcataccga agtaacaact
     1501 ccgttgatgg ccggcttgta ccagtctgtt gtgatcaata ctttcatctt ttttctccct
     1561 tccgtttatt ttctctgaat tgatctcagc ataacagaca caggtaaatt ccatccgaat
     1621 aaaatcaaaa gttttcctaa agtttctgtg agttttttcc aaaatgtgca ctaaaaaaca
     1681 gcattctccg gaaatttccg gaaaacgccg tttttaatcg tctttctata aaattattat
     1741 ctttcttctc tgaagtacgc cagtcccaga cttgcggggg gctgattctc tctcttgctc
     1801 cgcatactgg tgatgaccag taccagtaag gtcacgacat aagggatcat cttatacagc
     1861 tcctgatact ccatatgctc gataccggtg ggcagataca gatacagaat atacagacca
     1921 ccaaacagga aagaagcaaa tacactgata ttcggtctcc agatagtgaa gatgaccagc
     1981 gcaatggcaa gccagcctct gtcaccgaac gcatcgttgg accagacgcc gcaggcataa
     2041 tccattacat agtacagtcc gcccagacct gcgatcatac atcccacgca ggtagcgatg
     2101 tatttgtacc gggatacgtt gataccggct gcatctgcgg tattggggcc ctcaccgact
     2161 gctcttaagt gcaggcctgt tctggtatga ctcaggacat acgatgccgc aaacgcaatg
     2221 atcactgcca gataagccag gaatccatag gataaaaaca gcttaccaaa ccagccaagt
     2281 tttcccgcaa aaggaaggga cttactgaaa taactgctgg tagcagacag tgcaatggaa
     2341 ggtacatcac tgtttaccag cttgatcagg gatccgccga agaagttacc gaagccaaca
     2401 ccgaaggtgg tcattgccag accggtcacg ttctggttcg cccgcagcgt aacggtcagg
     2461 aaacagtaca gcagtcccat cagcagggaa cccagcagac agcaaagcat aggaatcgca
     2521 atagcgagga atccgttcat atttgccgca ccgcctgctg atctctcata gaagaaggag
     2581 ccaatgacac cgcagatacc tcccacatac ataacgccgg ggattcccag attcaggtta
     2641 ccggatttct ccgtgatgat ctcaccggta cttccgtata acagaggaat tccctgtaca
     2701 atagctctcg gaataaatgc aagtaaaaat gtccacatga ttactgttcc tccttttctg
     2761 cctttttacg gaactgcagg cgataggtaa tgaagaactc acagccgata ataaagaaca
     2821 ggataatacc tgtcagaata tcggagtagg aatgattcag tccaaaattg gtagagatct
     2881 cactggcacc acgatccatg aaaatgatca ggaagctgga gaacaccatg gtcagaggat
     2941 tgaacttgga catccaggat accagtacag c
//
LOCUS       DAWDHD010000243         2963 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_2034_length_2963_cov_10.169188, whole genome shotgun sequence.
ACCESSION   DAWDHD010000243 DAWDHD010000000
VERSION     DAWDHD010000243.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2963)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2034_length_2963_cov_10.169188"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          856 a          611 c          854 g          642 t
ORIGIN      
        1 attcaggtag agtatggaac aacgtgacag aataggttat gtgatgccgg gacagatgaa
       61 cattctctac agtggggaac aggtagcggc tactttagag aaaaatgccg tggtgaccct
      121 ggctaccatg gagaccaagt acggatattt caagaaagaa atgcatgtgg atattgctgc
      181 ccgcaacatc gccccttata acaacagctt tgaagccctg gtcaaggatc tgaaaaaata
      241 taaaaagaac ggctaccggg tgctgctgtt gtccggttcc cgtaccagag ccagacgtct
      301 ggcggaggat ctgcggaaca ccgaggatgc cggactgaca gcagtataca cagaagaccc
      361 catgagggaa gtacagccgg gagagatcct gacctattac gggcatgtga acaagggctt
      421 tgagtatccc tggctgaaat tcgtggtgct gtccgagagt gatattttcg gagcggagaa
      481 gcgtaagaaa aagaagaaaa agctctatca ggggcagaag atcaatgatt tcaacgacct
      541 gaagatcggg gattatgtgg tacatgagac ccacggactg ggcatctata agggcatcga
      601 gaaggtagag gtagacaagg tcgtcaagga ttacctcaag atcgaatacc gggacggcgg
      661 caatctgtac attctggcca ccggactgga tgtgattcag aagtacgcct ccgccgatgc
      721 agcgaagaag ccgaagctga acaaactggg cggcaaggaa tgggaacaca ccaagacgaa
      781 ggtacgcagt gccgtcagcg ccgtggcgaa ggatctggtg gaattatacg ctgtccgaca
      841 gcagagcgag ggctatgctt ttggaaaaga tacggtgtgg cagcgggagt tcgaggaaat
      901 gttccccttt gaagagacag aggatcagct gaatgccatc gcggacacca aggcggatat
      961 ggaaagccat cggatcatgg accgcctgat ctgcggagat gtgggctacg gcaagaccga
     1021 gatcgccatc cgtgccgctt tcaaggccgt gcaggaggga aaacaggtgg tctatctggt
     1081 gcccaccacc attctggcac agcagcatta caacaccttt gtgcagagaa tgaaagattt
     1141 ccccatcaat atcgcactga tgagccgttt ccgcaccccg tcggagatca aaaagacggt
     1201 gaaggatctg gaaaagggca tggtggatat cgtgatcggt acccacagag tattatctgc
     1261 ggatgtgaag ttcaaggatc tgggtctgct gatcattgac gaggagcagc gtttcggtgt
     1321 ggcccataag gagaagatca agaaattaaa agaaaacgtg gacgtgctga ccctgacggc
     1381 aacccccatt ccccggacac tgcatatgag cctgatcggt atccgggata tgagcgtgct
     1441 ggaggaggcc cccaatgacc gactgccgat ccagactttt gtctgcgaat acaatgacga
     1501 actggtgaga gaggccattg tccgggaaat ggcccggggc ggacagatct actatgtcta
     1561 caaccgggtg aacaatattg cggatatcgc agcacagatc gcaaagcttg tgccggaagc
     1621 caacgtggca tacgcccatg gacagatgaa ggaacatgag ctggagcgga tcatgttcga
     1681 tttcattaac ggagagatcg atgtgctggt gtccaccacc atcatagaga ccggactgga
     1741 tatttccaat gtcaacacga tgatcatcca cgattcggac aatctgggat tgtcccagct
     1801 gtatcagctc agaggccgtg tgggtcgttc caaccggaat gcctatgctt ttctgatgta
     1861 caagcgggat aagatgttaa aagaagtggc ggaaaagcgg ctggcggcca tcaaggaatt
     1921 tacggatctg ggcagcggct tcaagatcgc catgcgtgat ctggagatcc gcggcgccgg
     1981 taatctgctg ggattaagtc agcacggaca tatggaggcc gtgggatatg acctgtactg
     2041 caagatgctg aacgaagcgg tgcagaacct gaagggcatt catacggcag tggatttcgc
     2101 tacggtgatc gatctggatg tggatgctta cattccggcg gaatacattg tcaacgagac
     2161 acagaagctg gatatttaca aacggatcgc cggcatcgag acgataaaag agcgggatga
     2221 aatgaaggat gaactgttag accgcttcgg caacatcccc aaggcagtgg acaatctgct
     2281 gcggatcgcc ctgatccgta tggcggccca cggtctggac atgacggaga tcaagggcaa
     2341 gaacgaacgc attaccttca acttccggcc ggatgcgaag atcgatcccc gcaagattcc
     2401 ggatttcctg aaaaagcata aggatgaatt aagctttact gcttacggca atccgttttt
     2461 tacctataag tataaaaaga cgggactggt ggagaccgat gcggagcttt tgatgagcag
     2521 aaccgaggaa ttattggaag aaatgaaaga attggtgctg gaaacagagc cggttcagaa
     2581 taattaagag agttttatgg gactttaaaa atgatacctt tttatcagaa cagggaacga
     2641 aaggattctg ataaggaggt attttttatg aaaggatatg tgactgccag cggatatatg
     2701 ggatatgtgg agaatgaata tatcttattt gccagcgagg atgattattt tgaatatgtg
     2761 gattcgttaa atttttaatt aatgtccagt gtctttaccg gggctgtgta tatgtgatat
     2821 agtacaaatg taaagaaaaa atgtaagggg tttcaacgct gcactgtaag gagtaacggt
     2881 gaaattctga taaagcattt ggcaaaatat gcagatggga gtttatatta tggaactgac
     2941 tgagaagaag gaatttgacc tgc
//
LOCUS       DAWDHD010000244         2913 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_2085_length_2913_cov_12.773618, whole genome shotgun sequence.
ACCESSION   DAWDHD010000244 DAWDHD010000000
VERSION     DAWDHD010000244.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2913)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2913)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2913
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2085_length_2913_cov_12.773618"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          669 a          788 c          565 g          891 t
ORIGIN      
        1 tccgtgacag gtatttatgt taacatatct gtcagacttt gtcaacaact tttttgaaat
       61 cttttcaagt ttctttcgag ctgttgtgtt gtctcggtgt tgcctcaaca acgaatctga
      121 gtatatcatc cgctagtgta cttgtcaaca ctattttttg aattttttgc acaaattatc
      181 ccctgctacg tcaattccgc ataaacaggg gatttttgcc atgtttctat tctgtttttt
      241 actattttac aaagatagga agcttttgtc cgagctgttc caccagatat gccagatagt
      301 tattttcata caaacatgta agtattctgc tgtctctggt ataatctacg atcacctgtc
      361 ccagggttcc caggtctctg aagatattaa atgcgtatat tgtccaaaca attagaacag
      421 tttccaacat tccaaccaca atacctagta atttgtccag gctatgtact ataggtagtt
      481 tcacgatcat ctttgccgag aaaaatacga tattaagaag atgatgcacc aaaccgatcg
      541 ccgccagcaa cagtattgtc aggatcacat tgaatatctt tccatcaaag taactgctca
      601 gaccattccc gatcaggaac agcaccacac aggtaatcag cagggagatc agagagatca
      661 gagattttac cattcccttc ttgtagccgt ccatcaccat gcagatcaat actactgcca
      721 tgatgaccag taataaattc agattcattg gtatactcct ttattttaac tgtatcgtat
      781 ctattgtact atctgtcacc agataatatt tgtaggaacc tcctgctgga agcagaagcc
      841 gggcactctt accgaagctg ccatggtatt tttccacacc gtccgttgta aagatctggc
      901 actccgattc gttgtagatc accatgtcat catccccgaa gaaaatatcc gtataatcca
      961 catcaaaata gtagctgccc acctttgctg ccgaagtatt gtacacatcc atgcggtact
     1021 tggtctccgc ctggtccgaa aggaatacca gtcctacata tttatcactg tagaaaatcg
     1081 actggatctc gtcgtcatat aggtattctc ccgccgtcac aggcttctgg gtacccgagt
     1141 agatcataag acggctgtct cctaccgcaa acgctgtatc attattcata aactgcactt
     1201 tcggcacgac caggtcggaa taagaatatg cgctgaccat ataatcgctc tgattctcgc
     1261 ccaccggtcc gaaattgtag aatgtcacat tactttttac tacgcctgca tccacataca
     1321 ggtaactgat tgccagaagc tctccgtccg gtgacaaact gacggatacc ggataacctc
     1381 catccgtcat atgcgcctgt cccgtgtagc tgttggtacc gtccgcttcg taagtcatga
     1441 tccatgccac atccgtatcc gccagcaccg ctgtcactct tcccgtttcc gctactgtaa
     1501 tatttcggat cggcatggtc gttgtaatag ttcccagttg cttgtccgta ttctgcacgt
     1561 agatctcacg gccgttataa ctgccgatag ctgccacatc gccacaagta gcgatcttca
     1621 catcctggat cacataagtc tgattccact ttacatttcc cttggcatcc gtacagtgag
     1681 cgccgtcttt actatatgta agaatactgt ttttcaaccg tatgtctgtg gcatccgatg
     1741 agacctcccg gctaatggaa gatacggtgt catatcccgt atagatatgt ctctttgcct
     1801 gtaccaccac aagagcaagt gccgctccca aagccgccac caccaacagc acccggtaca
     1861 cgattttcag ccggtgttta cggatctttt tcttatagtc atcctgactc ttttgccgct
     1921 tttctttttc cttcagataa ttcttgatat ctgctgccat gtatctgttt ccttatgtat
     1981 tatcgtaact attaagaatc gcattcgcaa aattactgcg gcgcagtctg ctttttgctc
     2041 atgtagttac agtataacac acttcccctt tttgaaaata agttttttac gggagaagga
     2101 tttcctgacc gatcttgata tcatcggggt cagcaatacc gttcagggaa cagatctcag
     2161 atactttact gttgctgccg tagttccgca gagaaatacc gatcagtgtg tcccctctct
     2221 ggatagtata atgtgtgggt tgagcaaccg cctccactgt aggagtctct gccactgcag
     2281 agtctgccgt ctgtgtcaca tcctgtattg tttcctgtgt cgtgttctgt tctgttgcct
     2341 ccgtcacatc tgtcgtattt tttgtgacat tgctgtccgc agaggttcca tccgctacac
     2401 tgaccgtatt ttcttccgtc ggatctacag cactgtttcc tgttccatcc attgatgcat
     2461 tttccgtgcc ttccggcatc actgtctctg ctgcagtccc agtagtattt cccgccgcag
     2521 cattttcctg tcgcagagca tcctccaact tgtcctccgc caccagcgtg ctgatctggc
     2581 tctgttcctc caccgcatcc ggcagtttct gctccatcag gttgctcatg gcctgcctgg
     2641 ctgtctcccc cagggattct ccggtgcttt cctgccgcat caatgccaat gccatcacgc
     2701 agagcagaac aaagacaccg gcagccgcca ttttcatccg acgcagtccg gtcgttggca
     2761 tgggtataat attatccggt tccctgcgtc tgttctctac cgctctgcga tattccacag
     2821 gcgcattctc cgtatgtccg gcggcacgga aggcatttca gctctcctgt ctctgtctgc
     2881 gttcctcctg ccgtttctgc accacttcgt att
//
LOCUS       DAWDHD010000245         2901 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_2096_length_2901_cov_8.540408, whole genome shotgun sequence.
ACCESSION   DAWDHD010000245 DAWDHD010000000
VERSION     DAWDHD010000245.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2096_length_2901_cov_8.540408"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          841 a          592 c          834 g          634 t
ORIGIN      
        1 gaaatgtaaa aacgaatatc gggaaggaat caccgtatgt cccgattgcg gagaaacgct
       61 ggtaagcgag gaagaattga cggactggac ttccgtgctg ttcggtgaac aggacgaaat
      121 ggagaaactg aaagacttcc ttacatacaa tgggatccgc aagagcaaac tttccttcga
      181 tgaggcggag caggtctatg aactgtttgt agatgcaaaa gaggaaaaac aggcgaagac
      241 gattgcccgg gtatttctgg aacagcagga aaaagagcgg acggaaaatg ctgctgagga
      301 agaggctgcg gtagatgaat tcctgatgcc ggagacagaa gaagcatcgg aggaagtcaa
      361 tcccggagaa ctgctgaaaa cagcttccgc ccagggtgga cttacttata tgaacagtgg
      421 agccaaagca gaggagaatc gttcctctgc ctgggtactg ctggtggtgg gaatcctcgg
      481 aatgcttgtc atgatcctgg gaattaccgg cgtacttcct ttgaacatcg gcaaccccta
      541 tatgttctac ggagtgatga gtgctgtttt cattctgttc attgtcatgg gcgtggtatc
      601 catgcggaat gcaaagcttt ttgagaaaaa ggcagagtcc gagaactctc tggtggatac
      661 tcttctgaag tggagtgaag aaaatctgac ggcggaaaaa attgacgctc agattgaaaa
      721 tgctgcagat acaccggaag aggctcttta ttacaagaga ttcgaagtaa tccgcagcca
      781 gatgaatcat cagtttatga atctggatca gcagatgctg gagaacctta ttgacacaaa
      841 gatctatgaa cagatcttcc cggatacaaa cggagcagaa gaatgatcaa aatatccatc
      901 gtatgtgtag ggaagatcaa ggagaaattt ttccgggacg ccatagaaga atacagcaaa
      961 cgtctggggc gttatacgaa gttaaatatc atcgaagtga cggatgaaat gacaccggat
     1021 ggagccagtg cggctctgga agatcagatc cgtgaaaaag aaggggcgag gatcctggcg
     1081 aaactgccac aggatgccct gatcgttacg ctggagattg ccggtaaaaa cttttcttcc
     1141 gaagaatttg cggcatggat tgaagattgc actgtcagag gaaaaagcca tatctgcttt
     1201 gtcattggcg gatcgcttgg tttgcatcca agtgtctgta aaagagcgga tctggcctta
     1261 agcttttcca aaatgacatt tcctcatcag ttgatgcgag tgatcctgtg cgaacagatc
     1321 tatcgtgcat tccggatcat tcagcacgaa ccttaccata aataaagagt aaaagtagaa
     1381 gtatatgaca aaaaaacaac gtgcattaga aattatagaa cgtctgaaaa aggaataccc
     1441 ggatgccgga tgcactctgg attacgatca ggcatggaaa ctgcttgtca gtgtccgcct
     1501 ggcagctcag tgtaccgatg cccgggtaaa tatcgtagtg gaaaagctgt atgagaagtt
     1561 cccggatgta gatgccctgg cagaggctcc ggtggaagag atcgaggcca ttgtaaagcc
     1621 ctgtggcctg ggtcacagca aagccagaga tatcagtgcc tgcatgaaga ttctgcggga
     1681 taaatatgac ggaaaggttc cagacgattt tgacgaatta ttgtctctgc ctggagtggg
     1741 cagaaaaagt gcgaatctgg tcatggggga tgtctttggc agacctgcca ttgtgacaga
     1801 cacccactgt atccgcctgg tgaaccgtat gggacttgtg gacgggatca gggaaccggc
     1861 aaaggtggag aaggagctgt ggaagatcat tccgcccgaa gaaggcagcg atttctgcca
     1921 cagactggta tatcacggca gagaagtgtg taccgccaga acccatcctt actgcgaaaa
     1981 atgctgtatc agtgacatct gtaaaaaagt cggcatataa aaggcggaca gggaaatcta
     2041 ctgccgcaga caggagcaaa gcctatgagg atgtatgaca ttatcatgaa aaaaagaaac
     2101 ggcggagagc tttcgaaaga agagatccac ttttttatcg agggctatac ccgtggagag
     2161 attcctgatt atcaggtatc tgcgctgatg atggcgatct actttcagaa gatgaccgag
     2221 aaggagactt atgaactgac catggccatg gcacacagtg gtgagatgct ggatctgtcc
     2281 ggaatccatg gctgcaaggt ggacaaacac agtaccggag gtgtggggga taagacttct
     2341 ctggcattga cacccatggt ggctgcctgt gggatcctgg tggccaagat gagcggcaga
     2401 ggcctgggcc ataccggagg gaccattgac aagctggaaa gctttccagg attttccaca
     2461 tcactgacca cggagcagtt tattgaaaat gtgaatcgga tcggcattgc cattatggga
     2521 cagaccgcag atctggcacc ggcggataaa aagctgtacg ccctgcggga tgtgacagct
     2581 accgtagata atatgtccct gattgccagt tccatcatga gcaagaaact ggcagccggt
     2641 gcagatgcca ttgtactgga tgtcaagacc ggaagcggtg cctttatgaa gagcagggag
     2701 gatgcttttg ctctggcgga ggaaatggtg aagatcggta agaatgccgg acgtaagact
     2761 attgccgtaa tctcggatat ggatcagccg ctggggcggg ccgtgggaaa tgccctggaa
     2821 gtgaaggaag ccattgcaac cctgcgggga gagggcccgg atgctgggtc taatacacat
     2881 ctccgagccc acgagacgga g
//
LOCUS       DAWDHD010000246         2900 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_2098_length_2900_cov_14.274517, whole genome shotgun sequence.
ACCESSION   DAWDHD010000246 DAWDHD010000000
VERSION     DAWDHD010000246.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2900)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2098_length_2900_cov_14.274517"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          808 a          623 c          731 g          738 t
ORIGIN      
        1 tcacttattt aatttgatag caaaacgcta tcttataaac gaacgcgtgc gtttataaga
       61 tagccttttg caatcaaatt aaataagtga aacagtgttt agcttttttg aaaactcttt
      121 caccggttca gccaccacac tcccagattc agataccccg cgaacgcaac ccacaccaga
      181 taaggcagca tcagccatgc ggcaggcttc gagatgcggt aaaaagcaac ggtagtggcg
      241 aggatcagga tccacagagc gaccagccac aaaaaggcga aaagatacca tcccaggttg
      301 aagaaccaga tggaccagaa aaaattaaag ataagctgta cagcatacag ggtgagggct
      361 ttcgtgtcct cgcctttttg catgacaacg aggtaagagg cgattcccat aaggacgaac
      421 aggatactcc ataccaccgg gaagagccac atgggcggcg tcagtggagg ctgattcact
      481 gtttcaaagg ctttcatgcc gtctttggtg atccaggcgg agagtccgcc tactgccaga
      541 gggatggcaa cggcagtgat gaggtttttc cagtgaatat tgtgaaattt gtcggagagt
      601 tgcatgggag cctcctttga aaatacgatt ttgcatgtgc caatatccgt gtatggtatg
      661 attagtattg tatgaaaatg aaataaaaat tatgtgtcta gctataaaaa gaggacaaaa
      721 atttgggact atcggcaaac ggaggttttg gtgtatgatt aagagtgcaa taaagcatta
      781 tgactatcag caacagtaaa tatccaagaa aaggtaatag gtaacataga tatgggcaca
      841 gaacacacac agggttccct gacaaaagaa gcgatccggc tggcgtggcc ggcggtctgc
      901 gaatcctttt ttatcgcact ggcgggcatg gtggatactt ttatggtgag tacgctcgga
      961 gccaatgcgg tggcagcggt agggctgacc acacagccga agttcctggg gctggcactg
     1021 ttcatcgcag cgaatgtggc aatctctgcc ctggtggcga gacgatttgg agagaagagg
     1081 cagcaggcgg ccaatgagat ccttgtcatg acaatctgct tctgcatagt ggcagccgtg
     1141 atcatcagtg cgctgacagt gggattcgcg gatcagatca tcgaattttg cggatcgagt
     1201 gaggacacgc acgccatggc tgcgagatat taccggatca tcatgggcgg catgatcttc
     1261 aacgtattgt ccatgggaat caatgcggca cagcgcggcg cgggaaatac gatgatcgcc
     1321 cttaggacga atctgatctc caatctggtg aatatctgcg gtaactacct actgatcaac
     1381 ggccatttcg gattcccggc gctgggaatc accggagccg ccattgccac ggtattcggt
     1441 acggtagtag catgcgtgat gagcatcatc tcgatctgtc cgaaagacag atttatcagt
     1501 attccgtaca tgattaaaca tcacatcaga ctgcggttag aaccgctgtt ggagattatc
     1561 aaagtcggtt attccgtgtt tatcgagcaa gtattgatgc gtatcggatt catgtcgact
     1621 gccatgatgg ctgcgaaaat gggtacggaa gccatggcgg cacatcaggt aggcatgaat
     1681 atcctcggtc tgacgttttc attcggtgac ggtatgcagg tagcggcggt agccttgatc
     1741 ggacggagtc tgggagaacg tgacccggag aaagcgaaaa gctatggagc tatctgtaga
     1801 cgtatcggta tgggaatctc tgttgcactg gcggttatct atttcttcgg tggggagacc
     1861 atctaccgga tgttctttag agaggaaaat attatcgcat acggtgtgaa tattattcat
     1921 tgtatttgta tcattgtcct gttccaggtg tctcaggtca tctatatggg atgcctgcgc
     1981 ggcgcgggag ataccgcata cacggcagta gcttctacga tcagcgttac tttgatccgt
     2041 acggcggcat cctatatttt cggatttccc ctgggactgg gaatgaccgg tatctggatg
     2101 ggcattctgg cggatcaggt atccagattc ctgttcgcgt cgattcggtt ccgacaggga
     2161 aaatgggtga caattaagat ataatgagcg caggtaatga gcttatgata ataacaaaac
     2221 tcccggagca atatcgcacc gggagttctt gctttcaaat aacatgcaat gattcactgt
     2281 gagttctatt tcgtcttcaa tgtctttttc atagcagtga aagtctctgc actgatcaca
     2341 tgctcgatac ggcaggcatc ttcggcagcc acagtgggat ccacaccgat ggaagtcagc
     2401 cagtccgtaa gaaatgtgtg acgttcatag attttctctg caatttcccg ccccttatcc
     2461 agcagggtga tgtagccttc cttgtccatt tcaatatatt ccccattgcg gagattcttc
     2521 atagcgacgc tgacgctggg cttggacacg cccagatcgt tggcaatatc aatggaacgg
     2581 accacaccgt tctttttctt cagcactaag attgtttcca gataattctc agcagattcc
     2641 tgtattttca tgtaatgacc atacccttcc catacatagc attttgaacc gaaatacctg
     2701 ccctacatat ggaaaattga ccatccgcag gtattacaat aagaatagca gaaaaggata
     2761 ggaaatgcaa gatttcaact taatgagaaa aaatatcaat aagaaattcc caagagattt
     2821 ctatgaaaaa aatcaaaaaa atatattgac agtaaaaaaa ggttagtata tactaaccac
     2881 gtgaattgag aatgaatctc
//
LOCUS       DAWDHD010000247         2891 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_2113_length_2891_cov_9.316643, whole genome shotgun sequence.
ACCESSION   DAWDHD010000247 DAWDHD010000000
VERSION     DAWDHD010000247.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2891)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2113_length_2891_cov_9.316643"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1639..1648
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          637 a          840 c          592 g          812 t
ORIGIN      
        1 gatcatggct tcatcgtttt tgccgcggca tctgtggatc cgtgccagtc ccttccaggc
       61 aaaaggattg gccgggttcg tggaaagtgc cttttcataa tccgcttttg ccgcctccag
      121 acagcctgcc gcattatgaa aatctccccg ctccaccagt aaaaattcac aagggtaggc
      181 atacagatat tccgtaatga tcggaagtcc tctccggtaa aatatggtct tgcccgcttc
      241 cagtttgctc agatattcct tccggaattt atctatggcg tcgttcatct gttcctgggg
      301 aagccccgta ttggtcagct gccattcata ggcatccagg atcacacttt tgacattatt
      361 atccttcgcc tgttgtaaca gttccttcag ttcctccgga tgtgtcagat ccatgtacac
      421 ctttgccatc atttcatagg ccttgacata ggttggaaaa taatttagac aggctctgcc
      481 gttctggatc acccctgctg cattccactg cctcttcgcc gcttccagtt cgttggcata
      541 tgcagcgtaa acctgataat ccccgatcag tttctgggat atctccgcac agcgttcata
      601 ctcttccatc tccacataga tctgtgcctt ttcgatcagc ataccgatat ccttcggagt
      661 tccggtctcg gctttttccg cttccgcaat ggcttcggcg aatttttccg gctgtacata
      721 gccataagcc cgatagcact gcatccggat catatgtgac tgtcggatcc ggtctctgtc
      781 tttttcctcc tcttcggcat ccgtatcccg gaggatcttt ttctccaggc tttcttccca
      841 tttggctgcc gtggaaattg ccttctcata ttctgccgct tctgccagca gttttgtcat
      901 aagtcccaga tattcctcac gcctgtccgc aggaactttt gcttccagct ccctgaccgt
      961 gcggattccc agatcggtcc tgccatcctg cagataacac cagcccagat ccagtcccag
     1021 accgggatct tggttctgcg catattcccg tttcatttcc cgttccagtt cggtgttgat
     1081 cttctgcagt gtctccagaa atgtgttgtc tccgcctacg gaaagaatct cttccgcaca
     1141 tttttttgcc tctttatagt tcccctgctc ataataccag tccgtcagac gcaggttcgc
     1201 catgtagtgt ttcggcagct tctcccgaag actctcataa atagcagacg cttctacgcg
     1261 cccttcattt ctccacagta tttccgccgt attgtaagcg atcagatcat cctccggata
     1321 ttttgcagac agtttcttta acaaagtaat ggcttctgcg gtctgtccct ttccttcata
     1381 gagggacgca cggcagatct ccataacagg gtgggtaatc cccagagcat ccccacatcc
     1441 gatcatctgc tccgcttcct gtaatttttt ctcctgcagc gcctgataag ctctgtcata
     1501 atattgcaga aactgatcgt aatccgcatc ttccgcacca cggaactcgg aaaaatccac
     1561 ttcttccccg gattcgcact tatgtaccat atagcttaca aactgtgcgc gaaggagctc
     1621 gctgaaggcc cccgcattnn nnnnnnnntt ctcatccaga agcttccaga ttgcggtcgg
     1681 cagcttataa tgttccatca gaaaacgaag cagataagtg gtgcagtttt cctcttcctc
     1741 cagagaaaga aagtcatccg cctcaaaaag cgctttccat ttttccacat cacagcggtc
     1801 cgtaatattc tcatagacct tccgaacacc acgtacccac tgtccagccg gagtatcgtc
     1861 ctccagcgtc ggttccgcct cctcgttctc ttctgcatcc ggcgttccgg catagcggca
     1921 ggcctcctca taagccatcc gcagtcgctt gaagccttcc ggatcatcct ccggattcgt
     1981 taccgtcagc ttgtcacgat aggcattctt cagtgccctt tcgtcttttg tctgttcgat
     2041 tcccagaatc tgaaacactt cgatcggttc cacgctgtcc cctccactgt aagtatcaaa
     2101 gttttcgtac caatagaaaa agatattgaa aaggagcaga aaaatctttc tgctcctgaa
     2161 tagtgatctt agaatgtaaa cttatctgca aagtaagcat ccagatcttt gatagcaaca
     2221 cgctcctgct ccatggaatc acggaaacgt acggtaacgc agccatctgt ctcggaatca
     2281 aaatcgtaag ttacgcagaa aggagttccg atctcgtcct gacgacgata tctcttacca
     2341 atgtttcctc tgtcatcaaa ttcacagttg tatttcttgg aaagctctgt gtagatcttc
     2401 tccgcaccct cgttcagttt cttggacaga ggcagcacac cgatctttac cggtgccagt
     2461 gcgggatgga aatgaagcac agtacgaaca tcgccgccct ctaactcttc ctcgtcgtaa
     2521 gctgcacaca ggaatgccag aaccactctg tctgcgccaa gagaaggctc aataacgtaa
     2581 gggatgtatt tttccttctt ctcatcatcg aagtaagtca tatcctcgcc ggaggtattg
     2641 gcatgctggg tcagatcata atcggttctg tccgcaatac cccacagttc gccccatccg
     2701 aaggggaaca ggaactcaat atcggtggtt cccttactgt agaagcacag ttcctcagga
     2761 gagtgatcac gaagtctcat ctcgtcttcc ttgatgccaa gagacagcag ccagtcacgg
     2821 cagaagcctc tccagtactg gaaccactcc atatcggtgc cgggctcgca gaagaactcc
     2881 agctccatct g
//
LOCUS       DAWDHD010000248         2891 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_2112_length_2891_cov_11.194288, whole genome shotgun sequence.
ACCESSION   DAWDHD010000248 DAWDHD010000000
VERSION     DAWDHD010000248.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2891)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2112_length_2891_cov_11.194288"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          870 a          589 c          790 g          642 t
ORIGIN      
        1 gagaatactt atatcgtctg gtggagcaga cgtcgggcgt agaggactag gaacggaaac
       61 gtgtatggat cgactcccag acggaggaag aaatcttacg tggaatccgg gaggcaaaag
      121 atctctcagc ctacgacaat ctgggaaatg ctgcctatct gcgggccaag gaagattatc
      181 tcatgggtat caatttttcc agagccctga cgttaaaata cggcaacacg gtaaaagggt
      241 atctcaaaag agaccgggca gtgatttctg tgggtcgtgt catgacctgt gtcctcggaa
      301 tgatcgtgaa cagagagcgg gagattcgtt cgttcaccaa gactcccttt taccgggtga
      361 tcggtaattt taaattacag gagaagccct ttactgcaga gtggagagca gtggaaggct
      421 cgaaatatta taattctccg gcactgtata aagagaacgg cttcaagaaa aaagaggatg
      481 ccgagcgtct gatcgcagag cttaccgcag aaccggcagg cccggtgact gtcaccggca
      541 ttgagaggaa aaaagagaac aagaatgcac cgcttctgta caatctggcg gagttacaga
      601 atgactgttc caaatacttt aagatcagcc cggatgagac tttgcggatc gtacaggaac
      661 tgtatgagaa aaagatgacc acttatcccc gaacggatgc cagagtcctg tctacggcgg
      721 tggcaaagga aatccacaag aatatcggtg gcttacgcag ctatgctctg gccactcctt
      781 ttgccaaaga agtgttagag caggggacct ataagaatat tgcgaagaca cgttacgtga
      841 atgataagca gatcacggat cattatgcca tcattccaac gggacaggga ctgaacaatc
      901 tggcaggtct ggcccctacg gcggcaaaag tgtacgaggt cattgtacgc cggttcctga
      961 gtattttcta tcctccggca gtctatcaga agttctctct gacagtgaag gtaaaggaag
     1021 aaaacttttt tgccagcttt aaggtactga tacaggaagg gtatctgaag gtggcgaact
     1081 tctcttttaa gaaggagaat acggcaccga aggaggataa agagaacgag aatgaaagcg
     1141 ctgatgaaaa atgtgatgca gagcttacca atcagctgat gcatctgaaa aaaggagatt
     1201 ctctgactgc ggatacctat gagatcaagg agggcgagac ttcgccgccc aagcgttaca
     1261 cttcgggaag cattatcctg accatggaaa atgcgggcca gtttatcgag gacgaagaac
     1321 tgcgggccca gatcaaggga agcggcatcg gcaccagcgc cacgagagcg gagattctga
     1381 agaaactggt gaatatcgaa tatctggcac tgaacaagaa aacccaggtg ctgactccca
     1441 cccagatggg agagatgatc tatgacgtag tatcggattc cataagacca cttttaaatc
     1501 cggcactgac ggccagttgg gaaaaggggc ttaccatggt ggcggatggt gtgatcacac
     1561 cggaagagta catgggaaag ctgacggatt ttgtgggaag gcgaactacc gctgtacgca
     1621 gcctgaacaa tcagggagtg ctatggagac agttccaggc tacagaagaa ttttatcaga
     1681 agaagacaaa gcaatccaat gccaaaaata aggaataaag atgaggagaa attatcatgg
     1741 aaaatattac gattgagaat ctgtatgatc tgaaggagac cattgcggca gatctgttca
     1801 ctgaggcaga atatccctgg gaggtgcttc ccagaatcca cgatttcatt ctggaactgg
     1861 gtaagagact gccggaagaa atttatgaaa aaagaggcga ggatatctgg gtggcaaaga
     1921 acgcaaaggt agctcctacc gcctgcctga acggtccgct gatcattgat gaagaagcgg
     1981 aggtccgtca ctgtgccttt gtccgtggca atgccattgt gggtaaaggc gcggtagttg
     2041 gcaattccac ggaacttaag aacgtggttc tgttcaataa agtacaggtg cctcattata
     2101 attatgtggg agacagtgtg ttgggcttca agtcccatat gggagccggt tccattactt
     2161 ccaacgtgaa gagcgacaag acgctggtgg ttgtaaagaa cggagaagag aagattgaaa
     2221 ccgggctgaa gaagatgggg gccatgttgg gagaccatgt agaagtgggt tgcaacagcg
     2281 tactgaatcc cggaacagtc atcggacgca attccaacgt gtatcctact tcctgtgtca
     2341 gaggggtgat ccctgccgga catatcttca aaagacccgg agatgtggtg aaaaaagaca
     2401 atatctaaaa attagatcat tattattcaa agcatcgatg aattttgatt gacgaatgtc
     2461 cttttatggg ttatgatatt tatcagtgca acgctttgac ggggtaaagc gtctgcgcat
     2521 tacagtaagc ggatcgcaag atccgcagaa aagaggacat ttattatgaa aaagaaatta
     2581 ttatctgtag tacttaccgc agtaatggca gcatctgtcc tgacaggttg tggcagcacc
     2641 gataacggaa ctgcttccac cgcagcagct accggcacag ctcagagtga agcagcagct
     2701 actagtgccg cttccacaga tggtaaggta tataacatcg gtatctgcca gctggttgag
     2761 catgaagctt tggatgcagc aacccaggga ttccaggatg cattaaaaga aaagcttggt
     2821 gataatgtaa aatttgatct gcagaatgca cagggtgagc agaccaccgc agctaccatc
     2881 tgtaatggtt t
//
LOCUS       DAWDHD010000249         2887 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_2117_length_2887_cov_11.827684, whole genome shotgun sequence.
ACCESSION   DAWDHD010000249 DAWDHD010000000
VERSION     DAWDHD010000249.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2887)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2117_length_2887_cov_11.827684"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          582 a          813 c          567 g          925 t
ORIGIN      
        1 gagcctgtgc ctgtactctg catccgtagg aattattctg gtaaggattg tgctggtatc
       61 tgccgccctg attcggacgc tgtccgcctg catttctctg tccctgtcca ctctgaccac
      121 cctgacgctg tccgggcatc ttggaattct gagggttgct aacgaaaata atctttttct
      181 tctttttcgt ctcctcagta gatttggtct cctcggaaac tttcgtttcc tgagcctttg
      241 cttcccttgc gggtgcctga gccttttcct cagcaggctt tgcgttttcg gtatttaccg
      301 gagctttctg tgctgcgggt gcagctgctt ttttctttcc gaggctgttg cgtaccagtg
      361 ctgccgcatc ctcttccagc gagctctgtg ctgcttttgc ctcaattcct ttttcctgta
      421 aaaaacttaa tatatcctta ctctgtagtt ccaattcttt tgcgagttca tgtactttga
      481 ttttcgccat gctttcctcc attctgccta accgggcaaa ttcgcttcca attgcttgat
      541 gagtgcatct gccagtcccg catcacacac acctaccgaa gatctcagat cccggccgat
      601 ggcccagccg agttcttcct tcgtgccata ttcatatacc gggacttcat aataggaaca
      661 tttgtcgtga aacaactttt tcgtattggc cgatgcatcc tctgcaacga tcaccaacat
      721 ggcagaacct tttttcacag cgtcctccgt ctggaattca ccgcttacta ggttacgccc
      781 cctcatcgca agtcccaaaa gggaataaat cttattctgt ttcaagctga tcaaactcct
      841 tttccagtgt atcatatacc tcaggagaaa tactcatctt gaaagaacgt tctaatcctt
      901 tattcttccg ggctttctgc agacattcct tctgtctgca tatatatgca ccacgtccgt
      961 tttttttgcc ggttgcatcc agacatatct catcttccgg tgttttcagg acacgcagca
     1021 tttctttttt gcctttcatt tctccgcagc ctacgcactg cctcagtgga atcttttttg
     1081 ccattgatta ttcctgctct ccatcaaaat cgtctacggt atcatcttcg taatcatcca
     1141 catagtcctc ataacggaag ccgggagcat ccttggcctg agtctcggac ttgatgtcga
     1201 tcttgtagcc ggtcagtctt gctgccagtc ttgcgttctg accttcctta ccgatagcca
     1261 gagacaactg ataatcgggt acgactacct ttgcggtctt ctcatcggga tctgcaaata
     1321 ctgctacgat cttggcagga gaaagtgcat tctgaatcag gttaccgggg ttctcatccc
     1381 agttcacgat atcgatcttc tcaccgcgca gctcttccac gatagcattg acgcgggcac
     1441 cattcatacc tacacaggcg ccgacagcat ctacattggg gttattgctc catacagcga
     1501 tcttggtacg gcttcctgcc tctctggcaa tgctcttgat ctctacggta ccgtccttga
     1561 tctcggtaac ttcggactcg aacagtctct ttaccagttc cggatgggta cggctgacat
     1621 tgatgcgggg tcccttcggg gtgttcttta cttccacaat gtaaaccttg atacgctcgg
     1681 tgggcttgaa tacttcgccc tttacctgct cgttctcggt cagcatggca tctgccttac
     1741 ccagattgat gctgatgttc ttgcctacat aacgctgtac tacgccggtc acaacatcct
     1801 tttccttctc gtagtactgg ttgtagatca cgcttctctc ttcctcacgg atcttctgta
     1861 agatcacgtt cttggcattc tgggtagcga tacggccgaa ctccttggac ttgatctcca
     1921 catggatcat gtcacccacc tgtgctctct tggaaatctc ctgtgcatcc tccagagcaa
     1981 tctgcaggca gtcatcctcc acatcatcct tcgtgggaac gacttccttc tcagcatata
     2041 ccagaaaatc gcaggtctct ctattaatct ccacatggac attgtcagcc tttccaaaat
     2101 gattcttgca ggctgtcatc agggagttct cgatggcttc aaacaaggtc tccttgctga
     2161 tctccttctc cttctccaga acgtccagtg cctccattaa ttccttattc atcatttcct
     2221 ccattccggc tttcgccttt tcttagtgta tggtttcttc ttattctaca agctataact
     2281 attccttcgc cccttggctc tgaatagtat caaaaatcta atgcaagacg gatcagggca
     2341 atgtctgctc tggcaaaagt tctcgggctg ccgtcttccg tgatcgtaat gttatctgca
     2401 ttgaagctct ccagaatgcc agaaaactct ttgcatttgt caatgggctt gaacagcttg
     2461 atctctactt cttcaccgat gctcttctgc aaatgcttat cctttttcag tgttctaccc
     2521 agtcccgggc tgctgacttc cagaatgtaa gcatccggaa tgaaatcagc cttgtccaga
     2581 gcttcggata atgctctgct gacattctcg caatccagaa tgttcacacc ttccggcttg
     2641 tcgatgtaag ctctcagata gtagtcactg ccttctttta catactccac atcgtagatc
     2701 tccacctgac ttacttctgc gatgggcgtc agcagttctt cggttctgtt ttcgtagtcc
     2761 tctcgtttgg acatgtaggc cctcctttac tgatatgagc tgtcgttaca acaacaagaa
     2821 agagtggcgc acgtccactc ttactctagt aatcatcttt aaactataca cagtatagac
     2881 cctgtgt
//
LOCUS       DAWDHD010000250         2868 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_2138_length_2868_cov_9.994312, whole genome shotgun sequence.
ACCESSION   DAWDHD010000250 DAWDHD010000000
VERSION     DAWDHD010000250.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2138_length_2868_cov_9.994312"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          862 a          514 c          808 g          684 t
ORIGIN      
        1 ttttcccggc ggacatgtgg aaacgtggga gaccatcaaa gaatcggtga tgcgggaaat
       61 atatgaggag accggactga cgattcaaaa tcccagattg accggcatct atcactggat
      121 gacaggggac atacggaatg tgggattttt atataagacg gcggaatatg agggagaact
      181 ggtttcctcc gaagagggaa aggtctactg gatttccgga gaacagtttt tgaagaagcc
      241 gctggcgccc ggtatggagc aggtgtggca gatgatgcat gatgagaatg cacaggaatg
      301 tttgcagaca ttgacggaag aaggcattgt atcaaagatc cagtaggata tttcggaggc
      361 aggaggcccg tgaaaacgga aagcatcaca aaggaaaccc ggtaggagga aaataaaatg
      421 atcaacaaat tacatatggc aatgatcgaa ctttaccatg gagatgcaaa gcgcactcag
      481 catttttgca aggtacacag ctatgcgaag ctgattgcgg aaatggagaa tgtggatgct
      541 aagaccttat ttattttgga gacgtcagca ctgactcatg atatcggcat ccatctgtgt
      601 gaagaaaaat acggaaactg taacggaaaa ttacaggaaa aagaaggccc ggtcattgct
      661 gaaaagctgc tgtcagatct tggtttttcc ggagaagttt ctgagcgggt acagtatctg
      721 attgcccatc atcataccta caacaatata gacggcattg attatcagat tctggtggaa
      781 gcggatttcc ttgtaaacat gtgtgaggac gaactgtctg aagaagcctt gcagaacgct
      841 tatcggaata ttttccggac ggaaactggg aaaaagatct gcagagagat gtatgatata
      901 gcatgacttt tttgcgaatg aaaagatcgc cggagaactt gacatgacga aaggagcatt
      961 ataccgccat tacaagagta aaagagacat ttttgactgc attgtagagc ggatggagta
     1021 gggagacagc gagcaggctg cagagtatga catgtcggaa gatgataaag agagtatgcc
     1081 ggatcagtac aagacagtgt ctctggagga atttgtcgaa tatagtaagt ctatgtttgc
     1141 atactggacg gaggatgatt tcgcatccgt gatcagtagc atggcacagg aatggataga
     1201 aaggcgatga tccggaagtg cttatttaca aaaagagatg atgatacaat aaaaaatgca
     1261 attgaaaaat aagaaaaaca gcttgcaaaa caaatggtac agtgctacta taaccacgaa
     1321 aaaaacaagt tggaggtgaa tttcatggac agttgcgatg gttatgggcg aagtagaaaa
     1381 cacggtgctt tttcctgcga gcagggtaca gctgtctgtg gaaagtgacg gccgtatttt
     1441 gatttgttgt gcatagagat ttatgcagag gaagcatcgt gtgatgcttc ttttgttttg
     1501 ccctttacgt tgcatttaaa agatgagaac gagaaaggag acttagacta tgaacaagaa
     1561 atattccgaa ctggcagaat tatttcgcag cgagaaaacg gatgaggaga ttctggattt
     1621 tttattgaat tatcataaaa acgatattgc cgatatgatg gcggggatga cagaggaaga
     1681 acggaagcga ttgtatcatc tgctgggagc gcagaaggtc gcagagatct tcaaatacat
     1741 tgaggaaccc cgtaagtata tccgggaact gtctgtggag agtgctgcga gagtgatctc
     1801 cgagatggat tccgatgacg ccgtcgattt gttggaagat ctggatccgg aagagaaaga
     1861 acagatcgtc cggatgctgg acgaggatgc aaaaaaagat gtgaagatgc tgctgtccta
     1921 tgatgaagaa gagatcggca gctgcatgac gacgaactac atctgtatcc cggagaacct
     1981 gactatcagg caggctatga gcgagttggt aaagcaggcc ggagagaacg acaatatcat
     2041 gaccatctat gtggtagatg aaaagggtgt tttcgccgga gccatcgatc tgaaggatct
     2101 gatccgggca agggaaaatg cgcatctgga agatctggtg ttgaaatctt atccttatgt
     2161 gaccgatcat gagaagatcg atgattgtat ggacaggatc ctggattatg cggaggactc
     2221 gattcccgta ttgtcagaag ataagaaaat cctcggtgtc atcacatccg aagacctgat
     2281 cgaaatggta gacaatgaga tgggcgagga ctatgcaaag ttagcaggtc tgaccgcaga
     2341 agaggatctg aaggagccca cacttcaaag catgaaaaag cgactgccct ggctgatcat
     2401 cctgctgttc ttaggcatgg cggtatcctc tgtggtggga ctttttgaaa acgtggtagc
     2461 agtgcttccc atcgtgatat gcttccagtc attggtgctg gatatggcgg gaaatgtggg
     2521 aacccagtcc ctggcagtga caatccgtgt gctgatggac gagaacctga caggaaaaca
     2581 gaagttccag ctggtggtca aggaagtaaa gaccggttcc ttaaacggag cactgttggg
     2641 cattatggca ttactttgtc tcggtgtcta tgtgcatctg ttcaaaggat atacctggat
     2701 gggagccttt ggaatctccg gctgcgtggg agtctccctc ttagtggcaa tggcgatctc
     2761 cagtgccgta ggtacggtga tccccatgtt tttccataag atcaaggtag accctgcagt
     2821 ggcatccggt cccctgatca ccacggtaaa cgatctggtg gcagtggt
//
LOCUS       DAWDHD010000251         2845 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_2165_length_2845_cov_11.627957, whole genome shotgun sequence.
ACCESSION   DAWDHD010000251 DAWDHD010000000
VERSION     DAWDHD010000251.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2845)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2845
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2165_length_2845_cov_11.627957"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          780 a          780 c          532 g          753 t
ORIGIN      
        1 ctcgatcagc accagcaccg ggagcttatg ctccttgatc tgcatctgga gtctaccaag
       61 ctcctcctgc gccgcattca gtcgtttttc cagttcctct ttttccggct tcaccgccgg
      121 aatccactgt ttcagcatac tgtaaccacc ctttcccgaa atcctcgttt actaacttat
      181 ataagtttag tgtataccat ttttaaaaaa tataaaagca attgggaaat atatttacaa
      241 atattatttt tccatccgtt ctctgatatt attgtagatt tcccatgtct gcgcatcatc
      301 tgcaccgttt agaataacgg tgctcccctc tacttccatg actacataag tatcacagtc
      361 gttatgagta tagcggatgt aatctgcgtg gatttcatcg cgaaaatgtc ccatggagta
      421 tttcaggttg ccaaaaccgt tcgtccggat tgtattcgta tcccggaaca tattctcttc
      481 gtaagcaatg ctttcgatag cgtcatattt cactgtataa tcctgccagc cctccgcctg
      541 aacagtaaat tcattttctc cgtaacagat ctcgattttt cccgtatata acgtccatac
      601 cgtaaatagc acacatgcga gcattataaa cacatagact gcaactactt tatgattgca
      661 gtgtactgcc agcccttttc cggtgcgcag cttctttcta taatagagat aagaatacag
      721 gatgctgcca aaagctgctg ccgtgatagc gacaataaac agaaataatg cagccatgct
      781 gtcactgaac agtccacaga tcatgctcaa tagtcctgct gccacccata ttttcccact
      841 gaagcggtgc gtggcattcc agttctcctc atcctgcaat gtccatacga cgcggatgcc
      901 gatggtgcgg ttcggtttca ccttgggcag ataattgccc acaataacaa atagcaatcc
      961 cattccgaca tacaaaatta ccatgagcag attctctttc cttacaccca tatcaatcca
     1021 tctggcgata ccgttataga gcaatgtcat tcccggcacg atccacgtaa ccattcttat
     1081 gaccttatca ctctgctgcc tgtttctgtt gtcgtaaaaa gttactgcca ttgctaccac
     1141 ataagtcact gcaaaagtgc cgtttaccca cagactgttc tcacaggtat atcccaatat
     1201 cattcctatg cacataacaa atgccgagca gatcagcctg cctttatttt tctgtatcat
     1261 tttcttcatt atccaatgct cctttcaaat ctgtgatcca taacatgatt tcttccagca
     1321 cggaagcatt cagttcataa taaataaact ttccttcccg cttgtcccgg atcagatctg
     1381 cctcctttag caccgacaaa tgcctcgata tggaagcccc cgtcaccgga aaatgttcgc
     1441 agatctctcc tgccgacaat cgaccgtttt tcagaagatt tagtatttca cgccttatag
     1501 gatctgccag cgctttcaat gtatcctgta ttcccaaaaa tgaatcacat cctttcatcc
     1561 aaacatttaa catttaacct aattgttaaa tatatcgtat catgcggatt atttaatgtc
     1621 aacagcttca cagatcaagg cagatactta ttcctgcgta tggatacatc tgtacataaa
     1681 aatccctccg tcttatgacg aagggatctc attcttctct atacttccgt accatcttcc
     1741 ggcagtcccc ttaagaacac cactgccacc ggcaatgaca aaaccgcaga taaaatatcg
     1801 gacaccggct gtgaccactg gatgccccga attccccaga acgccgtcag gatcagcagt
     1861 gccggaatgt agatagtgcc acttcggaag gaggctaaga tcgtcgccct gccgcccttt
     1921 ccgatggact gaaacagcat gttgccgcag atacataccg gaataaacgg cagtgtcaga
     1981 cactggatct gcaacgcata aaatgcgatc tcataggcat ccatatccgt aaggaattcc
     2041 aacaacagct ctctgcggaa cacatacacc acacctgcaa acactgccat ggtcaccaga
     2101 ctggcacaca gggtgaacca gaacgctttc cttacacggg aatacttctt tgccccgtaa
     2161 ttgaaggacg ataccggctg gaatccctgg ccgatgccca gtgccacact aaatacgaaa
     2221 ttgaaaatac gggacaccac gctcatggca gcaatggcaa tatctccgta gacagctgcc
     2281 tgtgcattca acaccatggt ggacacgctg gccagtccct gccgcatcat actggggaag
     2341 cctgctgtaa gaatattctt atgcacggca aattcccagg taaaatattt cggtgtcagg
     2401 ctgctctggg tctttccacg caggaaaaag aataacagga ttccaaagga tatgtactgg
     2461 gagatcatag tggaaagcgc cgctccccgg atgccaagat ttaagcccca cataaggatg
     2521 gcatctcccg ccatatttaa gataccgccg gagaccagtc ccaccatcgc aagattggca
     2581 tagccttcat accggagcac attgttcatc acacagctgg atgccattgc cggtgccgac
     2641 accagaatat agaaagcata gattctcgca tagggaagga tggtctccgt acttccaagc
     2701 aatctgcaca atccgttcag atccaaaata ccgatcaccg cgatcaacac tccggctccc
     2761 accgccagat aaaatccagt agcggaaaaa gctgtcgctc gcgccttttt ttgagctcat
     2821 aacaaccggc tgatattgat tccgg
//
LOCUS       DAWDHD010000252         2837 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_2181_length_2837_cov_10.164630, whole genome shotgun sequence.
ACCESSION   DAWDHD010000252 DAWDHD010000000
VERSION     DAWDHD010000252.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2181_length_2837_cov_10.164630"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          815 a          598 c          837 g          587 t
ORIGIN      
        1 caagtagaca gaccggtaaa accggcacag aataacggta atccgcagga tcccggctgg
       61 aagtccaaga acttcatgtc cgatgatgac gagttcgaat tcgaattcct gaactgggac
      121 ggcgacgaag aacaatagga ataaaaagac cggaaacggc atcttacgag agtgggatgc
      181 cgctttcttt ttaaaatttt gataaacttt cccggtctgc agttgacagc accgccctca
      241 ctgttataat agacgtataa caatcggtga cagtgggaag ggagtgattt tgatgataat
      301 agagatagat ttcaacagta atgaagcgct ctatatgcaa ctgcgcaatc agatcatcat
      361 gggaattgcc acgtcacagt ttcatgaggg agattccctg ccgagtgtac ggcagctggc
      421 ggataccatc gggatcaata tgcatactgt aaataaggcc tatacggttc tgaagcagga
      481 gggctatgtc aaggtcgaca ggcgaaaggg agccgtgatc gctctggatg tggaccgtat
      541 gcgtgcggtg gatgaattgc ggaaggaact gcaggtaata cttgctaaga gcagctgcaa
      601 aaatatttcc aggcaggaag tacatgaatt gattgatgaa atttatgaag aatatggagg
      661 actgaattga tgcagtcaca gtttacggat aaagcgcaga atgcgcttgc ccaggccggc
      721 agatgtgcca gaggcctgaa acagggctat atcgggaccg aacacattct ggtgggactc
      781 ctaaaggaag ataccggtgt agccgcaaaa gtgttggcgg ataacggagt agaagtggat
      841 caggtcatgg aaatgatccg ggatctgatc gcctttgaaa acggtgtggc cgtgaaggac
      901 aaagagggat attctcccag agctgcccgg attctggaag aggcacaccg ccaggctgcc
      961 cgtttcggac agaagcagac cggcacggag catctgcttc ttgcactgat caaagagggc
     1021 gagaatgttg ccgtgcgtct gctgaacacg ttgggagcca atgtgcagaa gatctatgta
     1081 gataccctga tcgccatcgg acaggacggc aatctgtaca aggaagatct tgggaaaaag
     1141 ggagaccgca aggcgaagca gtctacactg gaacaataca gcagagacct gacggctctg
     1201 gccagagaag gaaaattgga tcctgtggta ggcagagaag aagagatccg cagagtagtg
     1261 cagatcttaa gtcgccgcac gaagaacaat ccctgtctcg tcggagaacc cggtgtaggt
     1321 aagaccgccg tggtagaagg tctggcagcc cgcattgtgg caggagacgt accttttacc
     1381 gtgcagaata agagagtact gacactagat ttgtcgggaa tggtggcagg aagtaagtac
     1441 cgtggcgagt tcgaggaacg tatcaagaag gtattgaagg aagtgacgga ggacgggaat
     1501 attattttat tcctggatga acttcatacg atcattggtg ccggaggtgc ggaaggcgcc
     1561 attgacgctt ccaatatcat gaagccttcc ctggcccgtg gagagatcca gctgattggt
     1621 gccaccacca ttgcggaata ccgcaagtat attgagaagg atgcggctct ggagcgcaga
     1681 ttccagccgg tgactgtgga agaacctaca gaggaagagg cggtccgtat tctggagggc
     1741 atcaaaggaa aatatgagga tcatcaccat gtgcagatca ccccggaggc cattgaggct
     1801 gctgtcagat tgtccagccg ttatatcaat gacagaaatc tgccggataa ggccatcgat
     1861 ctgatcgatg aagcttcggc aagcgcaaga ctgagtgcat tggatgcccc ggataaagcg
     1921 aaggagatcg ctgaccgtat ccacgagatg gatcaggaga tggagagagc tatccgcatg
     1981 gaagcatttg accagatggc agagatcaag aagcagcagg atgcgctggt gaaaaaatat
     2041 gaccgcctga tgaaaaagcg ggagaaacag gaagcgggca atgttatcag tatcggtgag
     2101 aatgagatcg ccgaggtcgt tgcccagtgg accaagatcc cagtacagaa gctggcggaa
     2161 aaagagagcg aacgactgtt aaaactggag aagacgctgc ataagcgtgt catcggccag
     2221 gaggaagcag tgaccgcagt ggctagggcc atgcgcagag gccgtgtggg tctgaaggat
     2281 cccaaccgtc ccatcggctc cttcctgttc ttaggtccta ccggtgttgg taagacggaa
     2341 ctgtccaagg cattggcaga agccatgttc ggctctgaag atgccatgat ccgtgttgat
     2401 atgtccgaat acatggaagg ccattcggtc tccaagatga tcggatcacc gcccggatat
     2461 gtaggctttg aagaaggcgg acagctgagc gagaaggttc gccgtaatcc ctactctgtt
     2521 gttctgtttg acgagatcga gaaagcgcat ccggatgtat ttaatgtact tttgcaggta
     2581 ctggatgacg gtcatatcac ggactccaag ggacgcaagg tcagcttcaa gaacactgtg
     2641 ctgatcatga cttccaatgc gggtgcacag cgtatcgtgg atcccaagaa tctgggcttt
     2701 gccacagaga agagcgaaac aaaggattac gagaagatga aatccaatgt catggaagaa
     2761 gtaaagcgca gcttcaagcc ggaatttatc aaccgtattg atgatattat cgtgttccac
     2821 cagctgaaca atgagaa
//
LOCUS       DAWDHD010000253         2823 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_2198_length_2823_cov_13.070448, whole genome shotgun sequence.
ACCESSION   DAWDHD010000253 DAWDHD010000000
VERSION     DAWDHD010000253.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2198_length_2823_cov_13.070448"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          814 a          588 c          762 g          659 t
ORIGIN      
        1 agaagatgca gaataaggga atattgatgg tgatctccgg attctccgga gccggcaaag
       61 gaacactggt gaaaaagctg ttatcggaat atgacaatta tgcgttgtcc atttccatga
      121 ctaccagaca gcccagagaa ggcgaggagg acggaagaga atatttcttc cgtaccagag
      181 aacaatttga ggaaaatatt gcaaaaaacg gatttatcga gtatgctcag tattgcggta
      241 attattacgg aacaccgaaa gcatatgtgg aagagcagat gcaggcaggc aaggatgtga
      301 tcttagagat cgagatccag ggagccatga agatcaaaga gcagttcccg gagagcctgt
      361 tactcttcgt taccccaccc agtgcagaag agctgcagaa gagactggtg ggcagaggaa
      421 ccgagacagc cgatgtgatc gcacagagac tggcacgggc ttatgaagaa tcagaaggca
      481 tggatgccta tgattatata gtagtgaacg acgatctgga cgagtgtgct gcagcggtac
      541 agaagtatgt ggaagcagcg aaaaatgtac cgtcccgtcg tagagaattt atcaaagaga
      601 tcagagagga actgaaaggt ttcgcgaaag gagcaaaata aaatgttaca tccctcttat
      661 accgatttaa tgaaagtagt aaacagcgag gtagaacccg gtgaagcacc tgtagtcaac
      721 agccgttatt ctatcgttat ggcaactgct aagagagcaa gacagatcat cgcaggtgac
      781 gagcctctgg taacagccaa ggtggataag cccctgtcta tcgcagtaga agagctgaat
      841 cagggtaaga tcaagatcct tggtgatgat gaggaagcat aagacgcatg aagatattgt
      901 ttgtatccct gggctgtgac aagaatcttg tcgattccga gaagatgctt ggcatgctcc
      961 aggaaaaagg ttataccttc acggacgatg aagcagaggc agatgtagta gtagtaaaca
     1021 cctgctgctt tatcggtgat gccaaggaag aaagcatcaa tacattactg cagatgggag
     1081 aactgaagga cagcggacag gtcaaagcac tgatcgcagc aggttgtctg gcacagcgtt
     1141 accgtgagga aatccagaag gagatcccgc aggtggatgc cattatcgga accactgcta
     1201 ttgatgagat cgtagctgcc ctggaggaaa tccttgcagg ccagaaacag aatcattatg
     1261 aagatatcaa tgccgcccct gtgacagaga ccaaccgtgt tgtcactaca ggcggacatt
     1321 ttgcgtatct gaaaattgca gagggctgtg ataaacactg tacctactgc attattccga
     1381 aggttagagg caattaccgc agcgttccca aggagagtct gttaaaagaa gccacagagt
     1441 tggcggacaa gggcgtacgg gagctgatcc tggtagcaca ggagaccacg ctgtacggtg
     1501 tggatctgta cggtaaaaag gagctgccca gacttttgca tgaactggcg cagattcccg
     1561 gcatttattg gatccgtatt ttatattgct atcctgagga gatcacggac gagctgattg
     1621 atgcaattgc tgcagagccc aaggtctgca attatctgga tattcccatt cagcatgctt
     1681 ccgacaatgt gttgaaacga atgggccgac gtaccaatca ggcagaactt cgtgctatca
     1741 tcggtaagct tcgggagaag attcccgata tctgtttgag aacgactctg atcagcggat
     1801 tccctggaga gactcaggag gatttcgagg aactgtatcg ctttgttaac gagatggaat
     1861 tcgatcgtct gggcgtattc ccttattccc aggaggaaga tactcccgca gccaccatgg
     1921 aagatcaggt gccgcaggag gtcaaggaat ttcgcaggga tgaactgatg gaactgcagc
     1981 aggccatcgc ttttgataaa gcggaggata tggtaggtcg tatcctgact gtaatgatcg
     2041 agggcaaggt agcggacgaa gagacctatg tcgcaagaac ctacagagat gcccctaatg
     2101 tggatggcta tctgtttgta aatacttcag cgaatctgat gaccggagat tttgtcaagg
     2161 ttctggtcac cgattccaat gaatatgatc tgataggaga gatataccat gagtaagatg
     2221 aatctgccta acaagctgac tattttccgt gtgatcctga tcgtgccgtt tattatcctg
     2281 ttgctgggag gccaggccgg atggttcggc gataatacct tcgtgacgga tatgatcgcg
     2341 ttggctatct tcatcatcgc cagtctgacg gatctgatcg acggtaagat cgccagaaaa
     2401 tacaatctga tcacagattt cggaaagttc atggatccgc tggcggataa gctgttagta
     2461 tgttccgcca tgatcgcact gatcgagatg aaccgcatcc cttcctgggt ggtcatcatc
     2521 attattgcca gagagttcat catcagcggc ttccgtctga tcgcttccga caacggtgta
     2581 gtcattgccg caagctactg gggcaagttc aagaccacct tccagatggt tatgatctgt
     2641 ctgatgatcg ccaacttccc gcagttacag atcctcaccg atattattat gtggatcgca
     2701 ctgatcctca ccgtagtatc tctgattgat tatctcgtga agaacaaaga tgttatgaag
     2761 gatacgaagt aaatagaaag ttacgcgagc aatgagccgc gcataggcat agtgaagaca
     2821 gcg
//
LOCUS       DAWDHD010000254         2809 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_2215_length_2809_cov_11.308279, whole genome shotgun sequence.
ACCESSION   DAWDHD010000254 DAWDHD010000000
VERSION     DAWDHD010000254.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2809)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2809
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2215_length_2809_cov_11.308279"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          724 a          724 c          573 g          788 t
ORIGIN      
        1 gaaatctgct tatcattttg agtcgcttgt gttatacttt tattcatagc atgagcctcc
       61 ggaagtatga ttgtttctga acaactcaat tatacaaaaa cctgatggtt tcatgctatt
      121 tttttgccag ttattattga attttcaagg tgcgtaggca cttattcaag tgcgaagttt
      181 gagttattat attgtaacct attggttttt atacaaacga aaagaagttc ctgcaggaac
      241 ttctttcacc acaaatcata ttcatatttt cgagggcagt ccggttaaca aaattttcaa
      301 aataggggta caaacccctt tccaagtcca gcactccctt gattttcagg catttcagac
      361 aaaagtgcgg attctgggca aattatagca tattgttgca agtctattct tacaattcca
      421 tcagaaaatg ttcatgcagc gcacgtttca cctgatcgaa attacctcgc tccggacatt
      481 ggatggaaac aaccaatccc ttctctggga gaatcgttgt aatctgtccg aatgcgccat
      541 cagcacgata ggatcctgga tatggactga tccaaaactg atagccatat cctacatgcc
      601 agagatctgc agatggctcg aaggtaaatt tcggggtgga cacttcctgc acccagtccc
      661 tgctgacaat ctgctcgccc ttccagttac cctcagccag atagagctgt cccagtttca
      721 tcatctcggt acaagtcaga tacatacctc cgccgccatt cggatgtccc tgtggatcat
      781 gctcccagag cggccatccc atatcaaggg gctggaatac cttctcatac agatactctc
      841 ccatccgctt gccaacggcc cgttctacca tatgagcagc cagaatactg tcggcagagg
      901 aatagcagaa tgcactgcct ggctccacag gaaccggctg tgccatcata tatttctcat
      961 aatccggtgc gccgacgccc ggacgtctgt ccgcattcat cagaagtgcc ttaccaaagc
     1021 cactggacat gcagagcaga tgtttcaact gaatcttctc cagatttgga tccggatttg
     1081 aaggaagttt atctttaaac acttccgcca catgatcttc caggcttagt tttccctctt
     1141 ccattgcgag tccaactgct gttaccgtaa aactcttggt atgtgaataa atatttctcg
     1201 gtgcatttgc tgcgtagtgc atctcacaga gtaccttgtt ctcatctccg acactgatag
     1261 cttccagcgg cagattgtgg ctgctggcat aacgatgaaa ttccaaaaac ttctcttcca
     1321 gtaacataat acctctatcc tttccggcaa atctctgatg tcagtccgat ataaccgatg
     1381 cgacgtgtcg caaggttaaa accaatctat ctgacatctc caatctggtt ctattatctc
     1441 atacacatct ctgcatctgt cggcagtggc atcagcggtg cggacggcag ggtctgatac
     1501 caataagcaa cactggtgta atcatcgtaa cgcggtccgg tccagcccat gttgtcgagt
     1561 gtcatacgaa acttattctc aaaacggatc gggtctgcaa tatggaaatg atacagcagg
     1621 aatctctgct gtccgttata gaattcccgg ttgtctccat agattgcata catgccggta
     1681 tataagccgc tgaaggtctg atagctcttg atgatgatgt catttccaaa tccataggaa
     1741 ccgccgaagt agtcttcagt tccggtatag tggatagacg gatacggatc atcgtccaga
     1801 tacatgcggg cttcgccttc tacccaacag gtattgttgc cattcatacc agttgccaga
     1861 gtcacaccaa caaattgtcc cctgccttcg attccgtcaa cgattgtgta ggtacgaccc
     1921 ttctgaaccg ggtgctcttg gcgataggtg gcatggaaat aaccagcctc cgccggaatc
     1981 tcctgataag ctccggtgat aatataaaag agctcttcat ccttgtcacc acggttttcc
     2041 atcgtgatcc gagctctctt atggaacggc atctcaaaat agctgttata gccgcgacca
     2101 ggagctacca gcatcatggc agaattcaac accggatatt tgccatcccg gtccacgaaa
     2161 ttttcatcat aggcacatcc gaagaatgca gagagcggag cttccacaga cggatattcc
     2221 tgatcatccc aatacatacg gatgataaaa tggtgcccga catatccggt gaaccacata
     2281 tgctggatca cacccggtcc tgcagcatct accagagtta cggtttcccc tgcaggaatc
     2341 atcacggtcg gacgaagctt ggtgcagtca ccgccctggg aaccacctgc gcgtacaccg
     2401 gttggattct ctgcggaaaa tgcaaacgtt ttggagttct ggatcataca atagttgttc
     2461 atattcttct cctctctgat caaacctgga tcatctgaaa ttattgctta ctacgctatc
     2521 gcgtttctct attcactata aattcactat aacatttatc tcccatgaag taaaggaatt
     2581 acagaatcat tccgtctgcg ccttctcatt ttctttccgg ataatctcct ttaattcttt
     2641 tcgcatcaca tccagtggag ccttctgtcc ggtccataat tcgatctgag cagccctctg
     2701 gaactggaca taccaagacc attcaggatt ttgcagccct tcttctcagc atttttcagg
     2761 aatgcggtct tggacgagtt gcagcaggca tcaaaataaa actgtcctg
//
LOCUS       DAWDHD010000255         2739 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_2303_length_2739_cov_13.486215, whole genome shotgun sequence.
ACCESSION   DAWDHD010000255 DAWDHD010000000
VERSION     DAWDHD010000255.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2303_length_2739_cov_13.486215"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          791 a          604 c          757 g          587 t
ORIGIN      
        1 gacaatatca agatccgtac ggaagaggcg gattgtatgt attccggtat catgatcgat
       61 accaataact ttatgaccaa gacaggtgtc cgcaccttcg aggctgccgc attcctgcgc
      121 agaaacggtg cggatgtgac ccgtgtgaga aaactgttcc gtgaggatgc cgcagaatac
      181 aaggccaagg cggatgctgt cagtcaggca gagatctaca agcagttctt tgcaatctct
      241 ctgtgtaccg cagaggatct gcccagccct accatcattg gtgcgcaggc agccaacgaa
      301 ctgctgaata tcaagggcat taaggcaagt tttgtactta ccgattatca gggcaagatc
      361 tatgtcagcg ccagatccat tgatgaggtc aatgtacaga tcatcatgga gcgtatgggc
      421 ggtggaggcc acatgaatac cgcagcctgc cagatggaag gcgtgggact ggtagaggcc
      481 attggcgtat tgaagcacac catcgacgac atgctggaat ccggtgagat ctaagggatt
      541 ccgtttgaaa agttttacca tataaaacag aagattcagg aaaggcaggt tgatcctatg
      601 aaaattattt tacttcagga tgaaaaaaag ctgggaaaaa aaggagacat cattgaggcc
      661 agcgaaggat atgcaagaaa ctatatttta ccgaagaaga tcggtgtgga ggcaagctcc
      721 aagaatctga acgacctgaa gctccataag gcaaacgaag agaaggtggc acaggagaat
      781 ctggatgcgg caaaggcgct ggcaacagag ctggagacca agcaggtcgt ggtcaagatc
      841 aaggcaggag agggcggaag aactttcggt tctgtatcct ccaaagagat tgcaactgcc
      901 tgcaaggaac agcatggtat cgaaattgac aagaagaaaa tccagctgcc ggagagtctg
      961 aagaacttcg gctcctatga ggtaacagtg aagcttcatc cccaggtggc agcgaagctg
     1021 acggtgaaag ttacggaggc gtagacgacc ccatgcccgc aatggatgaa aatgtaatca
     1081 aaagaataat gcctcacagt ttagaggcgg agcagtcagt catcggctct atgttcattg
     1141 acgcggaagc cattgcggct gccacagagc agatttccgg agaggatttc tacaacaggc
     1201 agtacggtgt ggtatttgac accatcgtag agctgaacaa tgaaggaaag cccattgact
     1261 ttgtcacttt gcaggatcgg ttgcgatcca aggaggatgt gcctcccgag gtcagtggac
     1321 tggatttcct gacgaatctg attgcagcag tacctacttc cgccaatatc aagtattatg
     1381 ctggaatcgt ggcggagaag gcgacacttc ggcgtctgat ccgtaccaat gaagagattg
     1441 ccaatacctg ttatagtggc aaggagagcc tggatacgat tctggagagt accgagagca
     1501 agatcaatga agtggtacag aagaaaaacg tcaaggaatt cgtaccgatt cgtaccgtag
     1561 tcatgaacgc catggacaag atcgagaagg cttccaaact gcacggaacc gtcacaggta
     1621 ttgccaccgg attcattgat ctggactatc gtaccgccgg aatgcagccc tcggacctgg
     1681 tgctgatcgc agcccgtcct tccatgggta agacggcgtt tgtattgaat atcgcgcagc
     1741 atgtggcatt taagcagaat ctgccggtag ccatattcag tctggaaatg agtaaggagc
     1801 agctgatcaa tcgtatgttt tccctggaat ccagcgtgga cgcccagaag ctccgtaccg
     1861 gccagctgaa tgaccaggaa tgggaacgac tgatcgagag tgccggtgtc atcggcaagt
     1921 ccaagctgat gatcgatgat acccccggta tcagcattgc cgagcttcgt tccaaatgca
     1981 gaagaatgaa gcaggaaaac ggtctgtcca tgatcatcat cgactactta cagttgatgt
     2041 cgggaagtgg cggaagaagc agcgattcca gacagcagga gatttccgat atttcgagat
     2101 ccctgaaagc agtcgccaga gagctgtccg taccggtgct ggcattgtct cagttgagcc
     2161 gggctgtaga gcagagaccg gatcatcgcc ctatgttgtc tgacctgcgt gagtccggag
     2221 ccatcgagca ggacgccgac gtggtaatgt tcatctaccg tgatgattat tataatcacg
     2281 attcgccgga taaaggtatt tcggagatca tcatagccaa acagagaaac ggtcccatcg
     2341 gaaccataca gctggcatgg ctgccggaat acaccaagtt tgccaatctg gaaaacgaca
     2401 gaaaaaaaga tttctaagta tattacgaat acagaacata aaatacgaaa gagaacaagt
     2461 gagggagatt ccccacacct gttctctttc gtatttttat tatatgatgc cagggtcaaa
     2521 aggtctaagc ccacatgagc ttgctcatag gtaggtgaaa gaccttagga cccgccccga
     2581 tatgagcata aaagtgggat gataatccca cgatatgcga atattgggta gggtgctatg
     2641 tgccagtggc acatgtttag cacggaccga agcggagcgg agacgtggga gtgcgtagca
     2701 cggaacaatc cgtaggcatc aaagtcgggg gcttttgac
//
LOCUS       DAWDHD010000256         2733 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_2315_length_2733_cov_10.426811, whole genome shotgun sequence.
ACCESSION   DAWDHD010000256 DAWDHD010000000
VERSION     DAWDHD010000256.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2315_length_2733_cov_10.426811"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          729 a          584 c          770 g          650 t
ORIGIN      
        1 tatgatgatg cggtactgga atgcttcctg gacaaacagc tccagctttt tccggaagct
       61 gtagcagaga cactggatga ggcagaagat tttctggaag agtgcatggc agtcgtggta
      121 gattccgtgg aggatgtcat tgagtatttt gatgaggccg gcatggatgt ggacggcgct
      181 gtggatgatg agattctgga ggcagatgaa gtattcgata tcggagacgg cagatatctg
      241 atcgtagaag cgtagaaccg aagattattg tacacagaaa ctggaatgaa ccggagctct
      301 ccgacatata tatgttggag ggcttctttg cgtaagtctc ctgtaaaaat ctcttcccca
      361 tttgtaggga atgcgttata atgaaataat ggacttttac agaaaggtat ggtaaggaaa
      421 atgttagaag gacaaaaatg tctggtcatc ggctccggca tcagcgggat cggtgcagtg
      481 gctttattgg aaaaatatca tgcagacgtg gttctgtatg acagcagtga taaattgaca
      541 aaggaagatc tggaggcgaa gcttcccggg gaaagcaagg cagtctgtat cgccggagaa
      601 ctgccggagg aggtagaact gagcattcag gcaaccgttt taagccccgg agtacctacg
      661 gatattccgc tggtgaaccg tatgagggac cgtggggtac agattctggg agagatcgaa
      721 ctgggattcc ttgcggagca aggcagagtg atcgccatta ccggcactaa cggcaagact
      781 acaaccacca cactgaccgg tgagatcatg aaggcacact tcggtaagaa taagactttc
      841 gtggtgggta acatcggtaa tccctatacc ctggaagcgg ataagacttc aaaggacagt
      901 gtcactgtgg gagagatcag ttctttccag ttagagacca tacatacatt ccatccggtg
      961 gtatcggcga tcctgaatat cacaccggat cacctgaaca gacatcatac catggaggcc
     1021 tatgtggcag ccaaggaagc agtggcttcc caccagacaa aaaatgatat ctgtgtattg
     1081 aattatgaca atgattacac cagagatttc gcaggaagat gtcctgccac cccggtactg
     1141 ttctcctccc atcacgagct ggaaaacggt tacttcctgc ggggagacaa gatcattaag
     1201 agtgtgaacg gcgcagagac tgaactaatg gatattcaca aggatatgaa tctggtaggc
     1261 ctgtgcaatg tggaaaatgt catggcagcc attgctattg cagagggtat gcaggtgccc
     1321 ctggagacga ttcttactgt gatccgcagt ttccacgcag tggagcacag aattgagttc
     1381 gtagccacca agggcggtgt ggattattac aacgattcca agggaaccaa tcccgatgcc
     1441 gctattcagg gcatcaaagc catgagcaag cctaccgtgc tgataggcgg aggctatgac
     1501 aagcagagcg agtacgatga gtggatcgac agctttgatg gcaaggtaaa gtgccttgtc
     1561 ctcatcggcc agaccagaga gaagattgcc gaatgtgcca gaaagcatgg ctttgacaag
     1621 atccgttttg cggacactta tgaggaatgc ctgaagctgt gtaccgaact ggcccagccg
     1681 ggagacgctg tattgttgtc tccggcctgt gcaagctggg gcatgttccc gaactatgaa
     1741 gtcagaggac agcttttcaa ggaatatgta cgcggactgg cggaataaga aatcataaca
     1801 gaagtaagga gtaaaagcag gcatggcgga aacgcgggga agaagacgta aaaagaaaca
     1861 acaatccgaa tacttttttg actacagcct gctttttatt gtgctgtttt tactgggctt
     1921 cgggctgatc atgatctatt cggccagctc ctatgaggct tatgattcct atggagatgc
     1981 cgcctattat atgaagaaac agctgatcgc caatatcatc ggtctggtct tcatgatggt
     2041 cattgccaat atcccgtata ccttctggga acgatttgcc accctgggat atgtggtgtc
     2101 catgatcctg atcttcctgg taaagacacc cctgggtatc acttcccacg gcgctaccag
     2161 atggatcggt atcccacata caggcttcaa cctgcagcct gcggaagtag cgaaattgtg
     2221 tatgatcctg ttcctggcaa gtctggtatg caagatggga aaaagcgtcc gtaccatgaa
     2281 aggcttcttt accatgatgg cggcacccct gccgatcgca gcatcggtgt acctcattac
     2341 ggataatttg agttccgcca ttattattat gggtattgcg gtgctgatgg tattcgtggc
     2401 aagcccggat tacaagaaat tcatcatcat gggaggcagc gtactggcgg cggctggact
     2461 tttggtagtc gcagtcgtac agttgggaga taagatcggt ggcaagttcc gtctggcgag
     2521 aatccaggcg tggctgaatc cggaaagtca ggcgcaggat aaaggtttcc agaccctgca
     2581 ggcattgtat gccatcggaa gcggtggcat ctggggcaag ggactggggc agagtatgca
     2641 gaagttaagc ttcctgcccg aggcgcagaa cgacatgatc ttctccatta tctgcgagga
     2701 actgggattg ttcggagcgg ttgccattat tct
//
LOCUS       DAWDHD010000257         2732 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_2317_length_2732_cov_12.049309, whole genome shotgun sequence.
ACCESSION   DAWDHD010000257 DAWDHD010000000
VERSION     DAWDHD010000257.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2317_length_2732_cov_12.049309"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          705 a          608 c          732 g          687 t
ORIGIN      
        1 atctgcagaa tgcacagggt gagcagacca ccgcagctac catctgtaat ggtttcgtat
       61 ctgacggtgt ggatctgatc cttgccaatg caacctctcc tttacagagt gcagcagcag
      121 ctaccaccac gatccccatc ttaggtactt ccgtaacgga ttatgctacc gcactggaga
      181 tctccgactg gtccggtgct accggcagaa atatttccgg tacttctgat ctggctccta
      241 ttgaagagca ggaagctatg ttaaaggaac tgttcccgga tgtaaagcag gttggtctgt
      301 tatattgctc cgcagaagct aactctctgt atcagatcca gctgtttgag gctgctctgg
      361 acaaagatgg tattgcatat aaagagtatg ctatcgccga taccaacgaa gtacagtccg
      421 ttaccacggc tgctgcaggc gagtgcgatg ttctgtacat ccctaccgac aacacactgg
      481 catccgtaac cgagactgta tataatgtcg tagctcccgc taagattcct gttattgcag
      541 gtgaagaggg tatctgcaac ggctgtggcg tagcaacctt atccatcagc tattacgatc
      601 tgggatatgc taccggtgag atggcttacg agatcctggc tgagggcgca gacatcacta
      661 ctatggatgt acgttatgct cccaatgtta ccaagaagta caatgccaag atctgtgagg
      721 aactgggtat caccgttccc gatgattacg tagcaattca gtaagagtta tacaggtatt
      781 taagtacggt tctaacaaaa tatacttcga tagggatagc gcataacagc gatatcccta
      841 tctggagttt taggaggaaa aagaaatgtc ttactttatg tcactgaatc ccctttccct
      901 gttcagggca atgccgggat cgatagcaca gggaatgatc tggggagtca tggcactggg
      961 agtgtatatt accttccgcc tgctggattt cgcagatctg actgtggatg gttccattgc
     1021 aaccggtgct gcggtatccg ttatgctgat tcgtgcggga gtttctccta tagctacgct
     1081 gcccattgct ttcctggccg gagcattggc aggtatggta acgggactgt tgaacaccgc
     1141 actgggaatc cccggaattc tggcaagtat tctgacgcag atctccctgt attccattaa
     1201 tctgaatatc atgggcaaag caaaccagcc ggtcagcgta gataattatc cgctggtggt
     1261 atctttgcgt tatgtgacgg atggttcggt gagcagactt ctctttttcc tgggaatgat
     1321 cgtattttta ctggcactga tcgctgtgat gtactggtat tttggaacag aacagggaca
     1381 tgctatacgt gctacgggtt gtaacagcaa tatggcaaga gcacagggca tcaataccaa
     1441 ctttatcaag gtgctggcac tgatgatctc caacggtctg gtaggcctgt gtggtgcgct
     1501 gtatgggcag ttccaggggg cgtcggatgt caatatggga cgtggagcca ttgtcatcgg
     1561 tctggcagct gttatcatcg gtgaggttct cttcggtaag tttgccagca agagaaaact
     1621 tgcttttgca tttactctgg catcggttat tctgggtgct gtgatctact atatggtata
     1681 tcagttcgta ctgtggctga agatgccttc agaggatatg aaattgttct ccgctatcgt
     1741 agttgccatt ttcctggcaa ttccttactt gaaggagaag agaacacttc ggaaaggagc
     1801 gtcaaagtaa tggcttacgc attggagata caggacgtac ataaagtatt taacagaggc
     1861 accatcaacg agaaggtggc cttgaatgga gtaaacttaa atctgaatcc cggggatttc
     1921 gtgacgatca ttggtggtaa cggcgcgggt aagtccacca cgctgaatgc catcgccggc
     1981 gtatggccca tcgattccgg caagatcatc attgatggta cggatattac gaacctgcca
     2041 gaacacaaga gagcaaaata tctgggaaga gtattccagg atcccatgac aggaaccgca
     2101 gctaccatgg atatcgaaga gaacatggcg attgcgttac gtcgcggtga gaagagaacc
     2161 cttcgctggg gtgtttccag agaggacaga gagttattcc gcgagaagct gcagactctt
     2221 ggattgggac tggaagaccg tatgaccagc aaggtggggc tgttatccgg tggtcagcgt
     2281 caggccatca cactgcttat ggcagcattg aagcagccca agctccttct gctggatgaa
     2341 cataccgcag cactggatcc caagaccgca gccaaggtac tggagatcag cgacaagatc
     2401 atcgcagaga atcagctgac cgccatgatg gtaactcaca acatgaagga tgccatcgtt
     2461 cacggcaatc gcctgatcat gatgcacgaa ggcaaagtga tttacgatgt ggctggggaa
     2521 gagaagaaga atcttcaggt gaaggatctg ctggcaaagt tcgaggaagt cagcggcgga
     2581 gaattcgcca atgaccgcat gatgctggcg taaaaataag aacgggacac gctgccgatt
     2641 taggcagcgt gtttttgcgt taaatggaaa gttagtaaag aaatgctatc aaatccccgt
     2701 agcccttggc aaggtataag aaacgcggca cg
//
LOCUS       DAWDHD010000258         2684 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_2367_length_2684_cov_8.076075, whole genome shotgun sequence.
ACCESSION   DAWDHD010000258 DAWDHD010000000
VERSION     DAWDHD010000258.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2684)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2684)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2684
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2367_length_2684_cov_8.076075"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          734 a          552 c          729 g          669 t
ORIGIN      
        1 cgtgcgtgtt cacctttgac cctacaccgg cagtgctgtt cggcctgtct gacggtaaag
       61 aactgaccac cagagaggaa aaaagacgcc tgtttgaacg tctgggaatc gacactctga
      121 tcgagtttcc tcttacgaag gagactgcag ccacagagcc agagcgtttt gcaacagaga
      181 ttctggccaa acagatgaat acccggtttg tggcggcagg cgaggatctg tcttttggca
      241 agaacggcgc aggaaatgcc gaacttctgg agagaatggc gccgcatctg ggcttttgcg
      301 tccagaccat agagaagatt gaagtgaacg gcatcgaagt cagttcctct tacatccgca
      361 agctggtgga agagggccgg atggaagagg ccgaagagat gctgggaatg ccttatacac
      421 tggtgggagc agtggagcat ggcaatcata tcggacatac cctgggattt ccaacggtga
      481 atctgctgcc tcccgcaggc aaacttctgc cgcccaacgg agtttactat gcggcggtgc
      541 gccataatgg aaaggtctat aaggccatca gcaatgttgg aaccaaaccc acggtttctt
      601 ctgaaaaagt gatgggggtg gaatcttatc tgtatgattt tgaccaggac atctacgggc
      661 aggagatcga ggtggctttg aaaagatttc ggcgcccgga acgcagattt ggatcgctgg
      721 aagaacttaa ggagcagctg gactgggata ttgcggatgg tcgaaatgcg taggaaaaac
      781 agctgtttta cgcaaacacg gtagaaattg cgtaaagttt tgtaaagaaa agtaaaagaa
      841 aagtaaaatt tacattgcaa gtttttattc tatctcgtaa aatatatttt ggattgttta
      901 tataagaata aatttgaaaa ttattgtttt ggtttttcgg aagagaatca agagagattg
      961 agggattaaa aatgagcttt ggaacaatat tgacaatggc aggaggtctg ggactgtttc
     1021 tattcggaat ggagttgatg agtgattcca ttgagaaggt agcaggtgcc agacttcgtc
     1081 gtattctgga aatctttacc acgaaccgtt tcatgggtat gatcgttggt attattttta
     1141 ccggtatcat ccagtcctct tcggcatgta cggttatggt cgtcagtttc gttaactctg
     1201 gtctgatgaa tctgtatcag gcggcgggcg ttatcctcgg tgcaaacatc ggtactacca
     1261 ttacttcaca gctggtatct tttaatttat ccaaaatagc accgctgatc ctgctggtgg
     1321 gtgtggtcgt gatgatgttt acgaagaagg aaaaggtccg taaggtggcg gaagtggtcg
     1381 ttggtttcgg tatcctgttc gtaggcctga gcaccatgtc ccaggctatg gcgaacatga
     1441 agaatgagcc acaggtggtc aatctgctga tgtctctgaa gaacccgttc ttagccacat
     1501 tgatgggatt tgctctgaca gccattatcc agagttcgtc cgtaacggtc agcattgtgt
     1561 tgttgctggc taatcaggat ctgctgccgt tacctattac gctgtacatt atcctgggat
     1621 gtaatatcgg tgcctgcgct accgcaatgt tagcttccat gaccggtaaa aaggacgcaa
     1681 agagagcagc actgatacat ctgctgttca atattatcgg tacggttatt atttacattg
     1741 ccctgtttgt ggcaggagac cagatcgtgg aattgatcag gtccatttct gcagataacg
     1801 gccggttcgt agcaaatgca catacgctga tcaagattgc acaggtaatt atgctgttcc
     1861 cgttcaccgg ctggctggtg aagatgactt acctgatcgt acccggcgag gatcagaagg
     1921 ttggctacag agagagctat cagttaaaat acatcggcga caaagtggtg ttcaaccctg
     1981 ctaccgcagt ggtagaggtt atcaaagagc tggagcgtat ggcttctctg gcagaggaaa
     2041 acttaaaccg tgccatgaat gccctgatca cgctggatga agaggacatt gcggaggtct
     2101 acgaggtaga gaaaaatatc aacttcctga accatgccat cacggactat ctggtaaaga
     2161 tcaaccagac cacactgccc atcgaggatt tgaacagtct gggtgccctg ttccatgtgg
     2221 tgaacgatat cgagcgtatc ggtgaccatg cggagaacgt ggcggatgct gccagacagc
     2281 gtaaggaaga aggcgtttcc atcagtaagg aagcccagaa ggaactggga gatatgttgg
     2341 aaatggtcaa caagatcatc cgttatgctg tagaaatgtt cgccaagagc gatgaatccc
     2401 atatgcagga aatcgttact ctggaggatc aggtagacga gaaggaacgc gaactgcaga
     2461 agaaacatgt agagcgtctg accaagggag agtgttctcc cgaagccggc atgatcttct
     2521 ccgatatcgt atccggactg gagagagtag cagaccatgc caccaatatc gcttttgcca
     2581 ttaccacaga agaggatgcc gaagacggcg atatcaaacg gtaattaact ggaaagttag
     2641 ttaaatacat tggcaaaatc cacgaactcc cggagcatac gata
//
LOCUS       DAWDHD010000259         2598 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_2496_length_2598_cov_10.126229, whole genome shotgun sequence.
ACCESSION   DAWDHD010000259 DAWDHD010000000
VERSION     DAWDHD010000259.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2598)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2496_length_2598_cov_10.126229"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          683 a          589 c          689 g          637 t
ORIGIN      
        1 tcgctgtctt cactatgcct atgcgcggct cattgctcgc gtaactttcc atttaccgat
       61 aaaattccac cagagaatcc agctttgcgg acatattcat catcatggca tccaggacgg
      121 tgatggcggt catgcattcc atgacgacta cagctctggg gacgatgatg ggatcgtggc
      181 ggccgcggat ggtaagctct gtattttcac cggagcagtt cacagtctgc tgggggcgga
      241 agatggaagg agtgggcttc acgtaagctc ggatgagtaa ggggctgcca tcactcatgc
      301 cacctaaagt tccgccggaa tgattgctgg tcttgacgac tttaccgttc tccatgcgga
      361 agggatcgtt gttctcgctg ccggtatggc gggctgcttc ggcaccgtca ccaagctcca
      421 cagccttaac ggcaccgatg gacataatgg ccttcgccag attggcatcc agtttctcaa
      481 aaacagggtc tccgattccg gcgggaagac cttccacgag gcattccata cagccaccaa
      541 cggaatcgca gttttctctg gcctggtgca gataagcaca ggccttttcg tcagcctctg
      601 catccggcat ggcagtaatg gtggagcggg cagtgtcccg gttatatttt gcaggatccg
      661 catcgatggg gccgatggaa cgggtatagg cgaatacggt aatacccatc tgttccagaa
      721 tctttgcagc aatagctccg gccgccacac gcccgatggt ctcacgaccg gaagaacggc
      781 caccaccccg ataatcccga aaaccgtatt tggcatcaaa gcagtaatct gcatggccgg
      841 gacggtaatt ctctgcaatc tcgctgtaat ctgcggagat ctgggaggta ttgcggacca
      901 tcagggagat gggagtaccg gtggtctttt cgtcaaagac accggacaga atctccacct
      961 gatcggcttc tttacgttgt gtagtgatag cactggtgcc gggtttacgg cgatccagat
     1021 atttctgaat ctcttcttcg gagagaggaa gtccggcggg acagccgtcc acgacgacac
     1081 ccagtgcctt tccgtgggat tctccccagg tagtgatctt gaaaatgtta ccaaatgttg
     1141 aaccggccat gacagttctc ctttttccac tgttcagaac ctcacccatg cattcgcaaa
     1201 agcgcacgat gaattgtgcg ttccggttgc tgcacagtta attttgctca tttatggagc
     1261 ggtgactcta ttcgagtcac cttacgaatc ataaaactgc tatttacatg caagcatgac
     1321 atatcagttt taacgatcct tagaggtcag gatagttact ttttatcata aattatacgc
     1381 aattactgaa aaaatcgcaa cttttttata gaaaaaggat gcaacacaaa acaaaatatg
     1441 ctatcatatg acataagtac taacgggaag taaaaggtcg acacgggaat ggaaaaaact
     1501 acggaagaaa aagccagagc gaaacttctg aaatttggca gaaagaacaa atggaacaga
     1561 gcggtagcag tgccgctgct gttttttgtg atgtcttttt ttcatatccg gaattattgt
     1621 cacaacaacg gaaagcgttt tgcagtgatg ctcggtacct ttttcctgtt tgcggtttat
     1681 agcagctttt cttttccggc ttttatcacc gaggataccg gaacggatta tgaactgaaa
     1741 aataccgaca cccagaacat ctctctggca gaggaaaagg aaccggatat ggcaggtctg
     1801 gaacttttgg aggatgagga tgtgttggaa cagtcggatt acaacagcac ttcccatggg
     1861 tatgcactgg tggataagta tgacgcttcc gatattctgc aggcgtccga ggctattgtg
     1921 agcgagaatg cggatgctga gaagaccgga gaagacggca gtgtgacaga agacgataac
     1981 gcagagcagg aggaattccg ttccgatgac tggcgtctgg ttctgatcaa caaacagaat
     2041 tccattccgg atgattatac gttccagcta ggtactatca aaggaaagat gcagtgcgac
     2101 aagagaattc tggaagatct gctggctatg ctggaagcgg cggaagagga cggtgtgaat
     2161 ctgaccatct gctctccata ccgggatctg gaatatcagc agatgttgtt taaccgcaag
     2221 atcgagcgct atatgaagcg tggtatgtcc tatatggagg cgtatcagct gtccagtcag
     2281 gcggtgaccg taccgggagc cagtgagcat cagatcggtc tggcactgga cattgtctgc
     2341 aacgactata tgtcgctaga cgaaggcttt ggagatacga aagcaggaaa atggctggca
     2401 accaacagct gtcgttttgg ctttatttta cgataccctg aaggcaagga aaacatcacg
     2461 ggaatcgaat atgaaccctg gcattttcgt tatgtgggca aggccgcagc aacggtgatt
     2521 atggaacagg gaatctcgtt agaagaattc tgggaggaat atgtagaaga tgtatcgatt
     2581 gattaaacag gaaggacg
//
LOCUS       DAWDHD010000260         2587 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_2516_length_2587_cov_11.550158, whole genome shotgun sequence.
ACCESSION   DAWDHD010000260 DAWDHD010000000
VERSION     DAWDHD010000260.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2516_length_2587_cov_11.550158"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          759 a          580 c          679 g          569 t
ORIGIN      
        1 aacggtatga atacccgtgt catcacggag ctggcagagt actatgagaa ggagatcggc
       61 tatcagatcc cgcccagaac cccgtttgtc ggcaagaatt tcaacgtgac cagagccggt
      121 atccatgcgg acggtctgct taagaacgaa gagatctaca acattttcga taccgagaaa
      181 ttcctgaatc gtccggtatt gtgcgctatt tccaacacct ccggtctggc aggtatcgca
      241 cactggctga acacctatta taaattaaag ggagacaagc aggtacagaa gacttccgaa
      301 ctggtagcgg agatcaaaaa gtgggtagac gaggagtacg ccggtgggcg tgtcaccgtg
      361 cttacggatg aagagattgt ggcctgcgtc aaccgtatct gcctggagaa gaatctgaag
      421 atcggcgagt aagtatagaa tgagcatgac aacggtaaca gcgttgtctg gaatcgagga
      481 ttatagtgag taagtatgat gtaaagcagg aagtaacgga caagttctcc ctgcggggac
      541 gggtatttca caagctgaga gaagacatct taagcggaaa atatcaggaa catgaagaat
      601 tgaaggaagt ggctatcggc gaggagatgg gagtcagcag aacccccgtc agagaggcgt
      661 tccgtcagct ggagctggag ggactgattc agatcatccc caacaaggga gcctatgtca
      721 ccggtattac tcagaaggat gtgaaggata tttacatgat ccgttccctg ctggaaggtc
      781 tgtgtgcccg ttgggctacg gaacatatca ccaaggagca gatggaagag atggaagaga
      841 atgtctatct gtccaagttc catgcccaga aggggcatct ggagcagctg gcggagttag
      901 ataatcgttt ccatgatatt ctgtatgagg catgtgacag caagatgctg gagcatcagc
      961 tgaaggactt ccatcagtat gtcctgcgtg tccgcaagaa aacattggcc agtgccaacc
     1021 gcggtcccaa atccaatgaa gagcatgagc agatcatgga ggccatcaag gcaggcaatg
     1081 ccgatctggc agagcaactg gcacaccagc acatgatcaa cgcctatgac aacatggtga
     1141 aaaacggtct gaacgaggcc tatgcacagc aggataagcc gcaggaatag gttgcctgtc
     1201 tgtgaaaatt actgtagaat gtaattattc agagccaatg ggcgaagtaa ctatatgagt
     1261 aaataacatg caggcatgtt attcggtgaa gccttgcaga agcgattttg ccaatgtggt
     1321 tctggatagc tattgcaaag taaaagaata aataccggaa aggaaatcat tatggaaaag
     1381 atcaaaatga caacccctct cgtagagatg gacggagacg aaatgaccag aatcctctgg
     1441 cagatgatca aggatgaact gttactgcct tacatcgatc tgaagacaga gtactatgat
     1501 cttggactgg agcatcgtaa tgagaccgat gatcaggtaa ccgtggattc tgccaatgcc
     1561 acattgaagt acggcgttgc tgtaaaatgt gcgaccatta cccccaacgc tgccagaatg
     1621 acagagtata atctgaagga aatgtggaag agtcccaacg gaaccatccg tgcgatctta
     1681 gacggtactg tattccgtgc accgattctg gtaaagggta tcgttcctta tgtcaagaac
     1741 tggaccaagc ccattaccat tgcccgtcat gcttacggcg atgtgtataa aaatactgag
     1801 atcaaggtac ccggccccgg taaggcagaa ctggtattta ctgcagcaga cggcaccgag
     1861 acccgtgaac tgatccataa ttttgatgga gcaggtatca ttcagggtat ccacaatacg
     1921 aataagtcta tcgagagctt tgcaagagca tgcttcagct atgccgtaga tacgaagcag
     1981 gatctgtggt tctccaccaa ggacaccatt tccaaaaaat acgatcatac cttcaaggat
     2041 attttccagg agatctatga taacgagtac aaggcaaaat tcgaggaact gggcattaca
     2101 tatttctata ccctgatcga tgatgccgta gcccgtgtca tccgttctga gggcggattc
     2161 atctgggcct gcaagaacta tgatggtgat gtaatgtccg atatggtagc taccgcttac
     2221 ggcgatctgt ccatgatgac ttccgtactg gtatctccca gcggtgtcta tgagtatgag
     2281 gctgctcacg gaaccgtaca gcgtcattac tacaagcact taaagggcga agagacctcc
     2341 accaactcca tcgctaccat ctacgcatgg accggagctc tcagaaagcg cggtgaactg
     2401 gatcagatcc ccgaactgtg caacttcgca gacaagctgg agcaggcatg catcaagacc
     2461 atcgaggacg gcaagatgac caagagcctg tccctgatct ctacctgtga gcatcctgtg
     2521 atcctcaaca gcctggactt catcaaggct atcagagaga ccctggacag cctgctgtaa
     2581 attgaga
//
LOCUS       DAWDHD010000261         2577 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_2531_length_2577_cov_14.674068, whole genome shotgun sequence.
ACCESSION   DAWDHD010000261 DAWDHD010000000
VERSION     DAWDHD010000261.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2577)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2531_length_2577_cov_14.674068"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          722 a          564 c          712 g          579 t
ORIGIN      
        1 atgcagatgg gagtttatat tatggaactg actgagaaga aggaatttga cctgctctcc
       61 ctgggagaga tcatgctgag attatcaccg cccgacaatg agcgtattac cagaggcgac
      121 agcttcagca agcaggcagg aggcgcagaa ctgaatgcca ttaccggtgc ggcaatgctg
      181 ggattgcgct gcggtatcat ttccaagctt ccggccaatg acctgggtgt atatatcaag
      241 aaccgtgtac gtctctgcgg tgtcagcgat gactatctgg tatacgatga tgacaaggat
      301 gccagattgg gcgtgtacta ctatgagaat ggtgcttacc ccagaaagcc ccgtattgta
      361 tatgaccgta agaatacctc catcaacaag ctgaccgttg atgattatga tgacaagatc
      421 tacagcgata cccgctgctt ccataccagc ggcatcacac tggctctcag cccggaactg
      481 agagctaccg ctgttgagat gatcaagcgc tttaaagcac agggggccat catctccttc
      541 gatgtgaact tccgtgggaa cttgtggacc ggcgccgagg cgaaggaatg catcgaatcc
      601 attctgcctt atgtggacat cttcttctgc tccgaggaca cggcgcgtct gaccttcctg
      661 aaggaaggcg atgccaagag cattatgaag agcttcacgg aggagtatcc aatctccatc
      721 gtggcatcca cacagcgtat cgtacatagc ccgaagcgtc ataccttcgg ctccattatc
      781 tacagtgcca aggatgatac ctattatgag gaagcacctt acaccgatat cgaagtcgta
      841 gaccgcatcg gaagcggtga cgcttatatc tccggtgtcc tgtacggact gttggcacat
      901 cccggtgagt atcagagagc actggaatat ggcaatgcca tcagcgcact taagaacacc
      961 atccccggtg acctgctgtg caccgactta aaggagatcc aggggatcat caaggagcac
     1021 aagtccaccg gacgtattgc ggagatggac cgctaaagca tttacacttc ttatctgatc
     1081 gaacaaatag aaaacacaaa gctctgccat gttaatttaa gtgcatggca gggctttttc
     1141 tgtttatttt taacaaaatt gaacctaaaa tatattaaaa agtctgctgt acaagattaa
     1201 attagtttga tatactaaag actaatatag aactaaaagc aggatgttat cagacggaag
     1261 attcgagaaa cggaagggag acagcaattt ggaaaaggaa gaccatatcc ggcagaaaga
     1321 acaatggaaa caactgtctc cttctgaaaa atggcagtat tttatggact attatctgtg
     1381 ggttaccata ggtgtggcag cggggatcgc tattgttatt ttcctgatcg tacattttgc
     1441 caccaagacc agcttcgtca tgggagtcat ggcagtgaat acggacggag agcacatcga
     1501 agccaccggt ccggattatt ttacggattt cctgcgcgaa cacggtgtga tatccaaacg
     1561 ggatgtggtg aacctgaatt atacgatctg gatcgatccg aactcggatt ccgatgtgga
     1621 ttccacgaat ctcagcacga tccaggtatt gttcatgacc cggtcggtgg atgttttttt
     1681 ttctgacgaa gagtttttga agctcatggg cggcacggat tatctggcgg atttaaggga
     1741 ttatctgcct aaggaactat tggacagata tgaagatcag cagatcatgg tgttaaatac
     1801 catgacggga gaaacgatcg tggcggggat ccggcttgag aacaacgaat gggtaaggaa
     1861 gaccggatgg tatcaggaga ccgcagccgt ggggctggcg gatggcatga agaatccgga
     1921 gctggcagtg gcactgttgg aggagatcct acagtagttc cgtcacggct gcctgcacct
     1981 gcggcagatg atgcgctgtg ggaacgtgga tgacagagca cgatgggaac agaaagacat
     2041 ggagtataaa tagggcatgg aaaacatgga cgcaaaagga aatgacagaa aacactatcg
     2101 gggaatcgag aaacagacgg ataatccgtt tttgaacctg tatcacattg atgccctggg
     2161 acgggacgga acgccgtttc attattattt tgcgtcccga aatgatgaaa acaatatcaa
     2221 acacaagacc cacagtatgc gccccgaggg catggcggtc tacgcagtga cagaggacgg
     2281 agagaaactg gtgctggtgc gtcagtaccg gtatcccgtg aatgattatc tgtatgaatt
     2341 gccggcggga ctgatcgagc cggaggagac cgcttcggag gccgcctgtc gggaaatgag
     2401 cgaggagacc ggctggaaac tggaagtcta cgaaggcgga gaaccggctt tccgaagagg
     2461 atttttcctg gcacaggggc tgacagacga gagcggcagc atgatcttcg gaaccgtgac
     2521 cgagaatgtg ggacagcaga tggaaaatac cgaggatatt caggagatcc tggcaga
//
LOCUS       DAWDHD010000262         2559 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_2569_length_2559_cov_10.371006, whole genome shotgun sequence.
ACCESSION   DAWDHD010000262 DAWDHD010000000
VERSION     DAWDHD010000262.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2569_length_2559_cov_10.371006"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          721 a          555 c          739 g          544 t
ORIGIN      
        1 tggaggaaaa taatacatgc aactgcctat ttttgagaaa tacattgatg aactgatcga
       61 gaagagtact ttggatgttc ccgcatggaa catagagaag gcgagagagg ggaaagctgc
      121 cggctggaat tacattgacg gttgcatgat cctggcactt ttagagatct acaacgctac
      181 cggagagaag aagtattacg aattcgcaga tgcatttatt gaccacagag tacaggaaga
      241 cggtacgatt acgaagtatt ccgtggaaga atataatatt gacaatgtta atgcgggcaa
      301 gactctgttc gcattgtatg ccctgaatgg taaggaaaaa taccgcaagg cgatcgacct
      361 gatctacagc caggtggaga cccagccccg caccgaggaa ggcaacttct ggcacaagaa
      421 gatctatccc aaccaggtat ggctggacgg catgtacatg ggacagcctt tctacatgga
      481 gtatgagacc aaattcgatg acaagaagca ctatgatgac atttttcatc agtttgccaa
      541 tgttgtaaaa tatatgagag atcccaagac cggattgtac tatcacggtt atgatgcctc
      601 caaaaaggca ttctggtgta acaaggagac cggtctgtcc cagaatttct ggctgagagc
      661 actgggctgg tattccatgg cattgttgga cactctgagc aaatgtgagc cgggggaaga
      721 gtacaaggca gagtatgaca atctgaagaa ggtattcgtg gatctgatcg attccatgtt
      781 aaaattccag gacgagagcg gtatgtggta ccagcttcca gcactggggg gtcaggagcc
      841 gaattattta gagaccagcg gaagtgcgat catggcttac tccctgctga agggcgtgag
      901 actcggattt ttaccggagt cctacagagc ctatggatta aaggcatttc agggaatctg
      961 tgaccgttat ctgaaggaga aggatggaca tttaagcctc ggcggtatct gcctcgtggc
     1021 aggactggga cctgaggata accgtcggcg tgacggcagc ttcgagtatt atatgtccga
     1081 gcccatcgtg gaagacgatg ccaagggtgt gggaccgctg cttttggctt acacagaaat
     1141 gagaagactg gatggctaaa tgaagtcagg cggaatgatg aagaacgata acagaaatga
     1201 cagaagaaca tccggacctg cgaaagggac ggaaaagacc aaagcaacgg gaaaagccaa
     1261 tgcggcggga aaaactaaag tgacggcagg caaggaaaac cgggtgcctg taggacaggg
     1321 ccgggaaaag acggccgtta aaaagaagag tggcctctgc ccggtggctg ataaatgcgg
     1381 cgggtgcagc tggatcaaca agccctatgt ggaacagttg aagaataagg aaaagcaatt
     1441 aaaggagctt ctggctcctt tttgcaaggt cgagggtgtc attcccatgg agcacccgga
     1501 gcattaccgg aacaaggtac atgcggtatt cggcgagaac cggtatcacg atgccatctc
     1561 cggcatttat gaacagaaga gccaccggat cgttcccgtg gacagctgcc tgatcgagaa
     1621 tgccaatgcg gatgagatca ttgtctctat aagagggctg ctgaaatctt ttcatatccg
     1681 cccttacaat gaggacaccg ggtatgggct gctgcgccat gtgctgatcc ggaccggcca
     1741 tgttaccggc cagatcatgg tggtgctggt actggcgtcc ccaatcttgc cctctaagaa
     1801 taattttgtg aaggcactgc tgaaactgca cccggagatc accactgtgg tcatcaatgt
     1861 caacaaccgc aataccagca tggtattggg ggacaaggag catgtgatct acggcaaagg
     1921 ttatattgag gatgagttgt gcggaaagcg tttccgaatc tctcccagat ctttctacca
     1981 ggtgaatccg gtacagaccg agatcctcta cgggaaagca ctggagtacg ccggacttac
     2041 cggcaaagag accgtggtgg atgcctattg tggcaccgga accatcggca tgattgccag
     2101 tgacaaggcc ggcaaggtca tcggcgtgga gttaaatgcc gatgctgtcc gggatgcccg
     2161 caacaatgcc aaggcaaacc agatccgcaa catccagttc taccagaacg acgccgggaa
     2221 attcctcgtg gaaatggccg gacagggcgc gaaggtcgat gtggtcctga tggatccccc
     2281 cagaagcggc agcaccgaag aattcatgaa ttccgtagcc cggatcggcc cggagcggat
     2341 cgtctacgtc tcctgtaatc ccgagacgct ggtcagggac ctgaagtact ttaagaagaa
     2401 gggttaccgc gtgtcgaagg gcgtgggcgt ggacatgttc ccgttcaccg agcatgtgga
     2461 gtcggtagtt ctgatgcagt attgtggaaa ataagaaaaa tagtcatgtt agaccacaag
     2521 atgttgtggt ttgagggcaa aaattggagc aaaaaatga
//
LOCUS       DAWDHD010000263         2513 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_2653_length_2513_cov_9.027258, whole genome shotgun sequence.
ACCESSION   DAWDHD010000263 DAWDHD010000000
VERSION     DAWDHD010000263.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2653_length_2513_cov_9.027258"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          585 a          709 c          523 g          696 t
ORIGIN      
        1 ctcccggtaa ggtaaaacca ccttttccca ttctgagttc ctcctcatat tcctgcttat
       61 ttttcattcc tgtcatttct tcacttctat tttccagtac aatatctgtc ctgttacagc
      121 aattcctctt tcagacacag tctgaccaca cgttccagct gctctgcctc agacaacagc
      181 acctgatctc ctgcaggatc cggaatgtcc ttgtagatct ccggtttctt aggaacattg
      241 atttgtccca caggctccca tgcgccgaaa ccatccgtca cgcactctgc cagataagcg
      301 taggctcttt cagaatgacc tttgcatttc tcccggatct cctgtgccac atatcctgcc
      361 atcttataag caccgaagtc attggtatgg gtatagtccc cagggaaaaa gatgggtttg
      421 gcatcctgta agcccttttc caacacatat ttcttgctgt gggcatgaag atccagctcc
      481 ggaattccgt actgtgctcc cagttccaga catacatccg cgaattcctc cagcagatcc
      541 agataagtct gatcccgcag tctccaggta tttcttgcaa tgggtgtcac cagtaccggg
      601 tatactccct ttgcctgatt ttccttaata tagcgttgga ggtttgcctg atagccgcct
      661 ctggcctgca attccggaag cttctggtca ttgtggccaa actggtaaaa cacataatcg
      721 ccgcgtttac tgtactggtc gatcaccgca taatgtcctt ccttgcggaa agtatcggtg
      781 gtcagtcccg aatgggaatg atttgacacc gaaagtctcg cgtccagata agccggaagc
      841 atctgacccc agccacagta acaggtaccg ggataataag gataatctcc aggctgatcc
      901 gtcaccgtgg aatctcctgc caggtaaatg gtcggacaat tcatctcttc gatcatgagt
      961 ccactgatcc gtggtctgtc tgccaatact gcaatatcta cggtcttatc cgcaaaaata
     1021 tgggtctgtc ccgacggtac tatatcacag acatttaccg tcatggtata gacaaactca
     1081 ccggctccca ctgtgccatg aaaagccagc cgtcttcttc cggtgaagat cagtatctct
     1141 cccatttcct cttctgagcg gatggtcagg gtgatcctgt agttcccctg tctgggtaca
     1201 tctaccttga agatcagcgg aatcctcctg ggtgagccag acatcggttc tcccgactgc
     1261 ctctccaacg atgccacttc ttccgcagaa tccagatagc agccgttctc atcttccttc
     1321 aggaaggaca gctgttcgtt ttgataccag tacatcgtat caaaagagga attcagttcc
     1381 cggatctgca gcaacttctg ctctcttctg tttttttctg ttacaaaacc gtaacctttg
     1441 cccggctgat agaggtcttc gctgcccaca tcgatcagat tggctgttcc tccatccttt
     1501 ccgaacagga aattcctgcg aaacatctat aacgcaccat ccttctcaat atcatcggaa
     1561 attaacaagg ttgcaaagaa ggaaagtgcc agtccctgtc cccatccctg aatatacgcc
     1621 ttgggaatgc ctctgtatcc ttccttgtct ctcatgacgg cagttccgcc ggagacattc
     1681 atcaccttac catcctcgct gacattggca agcaggccct tgatggcctt gttgatatat
     1741 ttgctgtgta aaggattgcc tctggtcagc attgctgcgg caattcctgc agatgcggaa
     1801 atctcttcat aagattccgg atcatccact acggttctcc acaatccatt ctccgtctgg
     1861 tataccttca gggctgccaa ctgctcattt aaggagccgg ccacatcgat atacttcgga
     1921 tacagatagc accgaggcag aataccgcct acctgcgaca tggtaaatgc tgcccaggca
     1981 ttggctcttc cccaatagat gccggacata tgatctccgg caatattgtc atagccgtga
     2041 taccagagtc cactgcttgg attctgtaaa tatttgatat gccagtagta ctggttcaag
     2101 gcatcatcga tcagttcttc atcgtgttcc atcacgccca cacgcagcat caggaacgct
     2161 gccatgaaca aagtgtcaca ccagcactgc tccgggaaat cgttgttggc agatacggtg
     2221 tgctgcaata catgatctcc gaaacgcagg gcatcttttc ttaaataagc gatcttgctc
     2281 ataagaatgt catgatactg ctgctctccg gtggcctggt ataaggtgat caggcaatgt
     2341 cccatggcac aggcgtttac tgtccatacc ttgggcagtc ccagatcgat cagttcgtcg
     2401 atacgatcct ttaacagatc cagatattcc ttcttgcctg tcttctcata ggcatcgctg
     2461 attccgtaat atgccacacc acagggccag tcccaggtga gatccatatt cat
//
LOCUS       DAWDHD010000264         2474 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_2710_length_2474_cov_10.199669, whole genome shotgun sequence.
ACCESSION   DAWDHD010000264 DAWDHD010000000
VERSION     DAWDHD010000264.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2710_length_2474_cov_10.199669"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          556 a          676 c          508 g          734 t
ORIGIN      
        1 acacccagtg ccaggatgcc gtagatcatt ccttgttcca gtacgcttaa taataacgcc
       61 atatttttct ccattcttaa aatcctgtat atccggatta ctctattatt ttctgcctgt
      121 cggcagctat ttcctgatta ctgtactacg atctcatcaa aggactggta agcatcggtc
      181 ttgtaattct cgggaagttc caggttgatc ttctctgctg ctgcaaagtt cacatacagg
      241 ctgggctcgg agattacttc gaaattcagt tcggaagctt ctgcctctcc cttcaggact
      301 ttcgctgcca tcttaccggt ctgcttgcca agctcgatgt attccagacc catggatgcc
      361 acgcaaccag ccttaacctg ctctacttcg gaaccaaata cggggatgcc tgccttgtta
      421 gcctcatcca ataccgtctg cagtgcgctt actacggtgt tgtcggtcag gttggtaatg
      481 cagtctacct tggatgcgat atccgccgct gccagagcta cctcagacat tgcggtaaca
      541 ccgctgtcca cgatctcgaa tccgtagttg cctgccagag ccttgtactc tgcgatggta
      601 ctgatggagt ttgcttcgct ggtggtgtag atgataccga tggtcttcgc ttcaggaagt
      661 acctcacgga tcattttcaa ctgtgcatct actgccagtg catcggaagt accggtgatg
      721 ttgcctacgg gagtaccatc ctcattggcc agacctgctt ccacaggatc agatactgca
      781 gtgtagatca cgggaatatc tgtattcatt gtagcattgt atgctgccat agcgcaagga
      841 gttgcgatag cacagatcat atctactttc ttacttacga agttgctggc ggtcatggcg
      901 gaagttccgt tgtctgcctg tgcattgaca tattcgatcg tcaggttctc accctctaca
      961 ataccttcct ctgccagccc ctgaataaat ccttcacgac agttgtccag ggaaccatgt
     1021 acggcgtact gcatgatacc aatggtgtaa ttgtccttgc ctgcggtgct gccacatccg
     1081 gtgagggcac ccattgccat aactgcggtt aatactgctg ccaaaattct cttcttcata
     1141 atcttattcc tcgctttctg tacgtcctgc gtaccatgat atgcagcgca taccataaaa
     1201 tagtttgctc ataagattac tgaacctttc actgtcatga aagccaaatg ctttcgcatc
     1261 tgtacccaca ttcgtatttg catacaagcg tcatgtggat tgaaagactt tcataacaaa
     1321 aaaccgcctc aaatgtatat tgctatacac ttgagacggt aataagctct tgctcatcat
     1381 cgtggtgcca ctcaatttga caattcctgc gaattgtgtc cactctctct ggatactatc
     1441 atatccatcg gcttgataac gggtccgaat cccgctgacg cctaaaactg atctgttctc
     1501 gggccagcct cggaagtcca ttcggcgaaa gttcctttac cgtgatccca ccatccacgg
     1561 ctctctgtaa aattccctaa ggcttactac tcttcctcac aggtttggta aacttatgaa
     1621 tgttgtttat gtgtcttaga ataatctcta ttttctattt tgtcaatact atttttagaa
     1681 aatttacaaa tcttatccgt actgtcacat acttacattc gtaaacccaa cgaatccaac
     1741 gaacaactgt tactctgtct ccggcagagt gatcaccacc ttgaacaggt cgccgtccgt
     1801 gacaatctgc atatcaccat tctgcagttc cgtcaggctc ttggctatgg ataatcccag
     1861 accgttgccc tccatgtggc gggatttgtc tcctctggtg aaccgctctt ccagttcctc
     1921 cggtgacatc tccagcggga aggctgacat attccggaaa atgattctca catcctgacc
     1981 gtcatgctcc acgttcaggt agacgcggct gcctgcctgg gcatatttgt aaatattatt
     2041 cagcagattg tcaaacactc tccacagatg cctgccgtcc gccatgacct ttacggtctc
     2101 ctccggctgt tttacgatag tttccagctt cttgtcagag aatcgctgct cgtattcccc
     2161 cgccgcctgt gacaacagta ccgatacatc gcagacttcc gggtgtactt ccagattgcc
     2221 tgtggtggct ttggaagcct ccagcagatc atccaacagt tttttcaact tcttggactg
     2281 gcgtaacaat acttccgcat actccgacag acgctgttcc ttttcggaat ccgtctccgc
     2341 cgtcagacca ttctctgttg ctgcattctc ttccacttcc ttcccgatga gatccgcata
     2401 attaataatg gaagtcagcg gggtcttgat gtcatgggac acattcgtga tcagctccgt
     2461 cttcagatgt tcac
//
LOCUS       DAWDHD010000265         2365 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_2896_length_2365_cov_12.804762, whole genome shotgun sequence.
ACCESSION   DAWDHD010000265 DAWDHD010000000
VERSION     DAWDHD010000265.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2896_length_2365_cov_12.804762"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          675 a          453 c          692 g          545 t
ORIGIN      
        1 cgaatgaggt gcatctggta ctgatggata ttatgatgcc gaagctggac ggcattcagg
       61 ctatgatcaa gattcgggaa aggagtaacg tgccggtgat cctgctgact gccaagggcg
      121 aggattccga caaggtgctg ggacttacgg taggagcgga tgactatatc acgaagcctt
      181 ttaaccccat cgaagtatcc gccagagtca aatcccaact gcgtcgctat atgcagctgg
      241 gtggaggtac tgtccgaccg gatgtgataa gagtcggtgg tatcgaactg gatgacaagg
      301 agaaaagggt gatgctggac ggcgagctgg tagcactgac gcccacggag tatgagatct
      361 taaagcttct gatccagaat ccgggacagg tattttcccc gaaggagatc tataccagaa
      421 tctggaacga cctgcccctg ggcagcgaga ataccgtggc ggtacatatc cggcatttac
      481 gggaaaagtt ggaaatcaat ccggcagaac ccagatactt aaaagtagtc tggggccagg
      541 gatacaaatg tgagaagcag ggataagagc aggcaggtga gcggagcagc ttgaggaaat
      601 aaggagacag gaatatggat aacagggaag aaaacagcag gagcacagaa caggacagag
      661 tggcaaatcc ggtggaagaa acagtgagta cgacggtaga accagcattg tcagagaata
      721 aaagggaacg gaaaaaaaga gaaaaagcag agaaaaaaaa gaatgcaccg gcacagggaa
      781 acagcctgtt gggcagcaca atggcaaagg ttgtggcatt tgttctgatg atcgcagccg
      841 caattgtggg ggtgttggga ctctatggta cctgtatgct tgcagacagt ggagcgtatt
      901 cctccggtga aaggatgttt atccatgatc agctgttgta ccggcaggtc aatcaggatc
      961 tgtatcggat acgcgactgg tatgagaatg agagcttgca gaccatggaa gcttattgca
     1021 ggcagcggaa catctgggta aaggtggaga gctacgatct ggatacggat gaattcctgg
     1081 gagaatggaa gaacgaggat tatgtagaca agacaaatga tctgacttac cggctggagt
     1141 cccaggtgca gcataggaat gataacacct atatcaacac agaaacttcc tctgcctctg
     1201 atagctatga aagttacgga agctctgaaa gctctgatat catggagctt gaaccatctg
     1261 tcaataccgg gtctgatgtg gaaagcataa cggcagcgga gcaggaagcc ctttcctccg
     1321 gcaatctgga gaaatatctg gatctggggg acagcgtatc cttaggtgga gagatttatg
     1381 tgccggttga acgggcggaa caatatgcaa aatggatgat caaggatgcc ttaagtgaga
     1441 cagaacggca gcaggaagag gctgcagagg aagcattagc agagcgtaac aagcgtattg
     1501 tggttaccct gacagtgaat cccagcctgc cgaaggaaga cgaatatgcc ctgatctatc
     1561 atcaggcaga gcagttttat gataaccgaa atgtgatccc tgtggtatgc tgtacgggaa
     1621 cgatactggc attattatgc tttatcttcc tgttgtgcag tgccggacat aagaatggca
     1681 gggagggtat tacccccagt gcgatccatg atatccacct ggatgtgtat acagtggtcg
     1741 ttattgttgg agcttttacc ggtctgtacc tggcttttgg atggatcggt atgaactcaa
     1801 gtatgatcaa tctgatcgtt ctagtggtac tgtttgccgc agaggtgata tggtgcaccc
     1861 tgtactgcat ggagttggcc atccgcctga aaatgggaaa atggtggcag aatacgatcc
     1921 tgtatagagt gttccggttc ttcggaagat tctgcaagag agttttccac ggtattgtga
     1981 aattgatccg tgggattcct atggtatgga gaacagcact tttgtgcctt gcagtctgcg
     2041 tggtagagtt tttcggttta atactgtttt ataataatac agatgtatta ctctttttct
     2101 gggccataga gaaatttatt ctgtgcggtg cgattacttt tgtagcattg atgtgcaaag
     2161 aactgcagga gggcagtgaa gcattggcgg acggagatct gaattataag ctggatacct
     2221 ctcatatggg actgtccttt aaggaacacg gagagaatct gaaccgcatc ggcgagggaa
     2281 tttccgcagc cgtggaacag aggatgaaaa gtgaacatct gaagacggag ctgatcacga
     2341 atgtgtccca tgacatcaag acccc
//
LOCUS       DAWDHD010000266         2268 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_3099_length_2268_cov_34.160868, whole genome shotgun sequence.
ACCESSION   DAWDHD010000266 DAWDHD010000000
VERSION     DAWDHD010000266.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3099_length_2268_cov_34.160868"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          677 a          520 c          531 g          540 t
ORIGIN      
        1 ttcataactt gtttgtgcct tgagcgaagc gaaaggaatg acaaaaacta tgacattaca
       61 aaaagtactc agtcgtgtca gacaggccgt agaggattat catatgatag aggaaggcga
      121 ccggatcgcc gtggggatct ccggtggcaa agatagtctt actcttctgc tggcactcaa
      181 tgaattaaga aaattttatc cgcagaagtt cagcatttat gccgtaaccg tggatctggg
      241 atttcaaaat ctggatctga acgagatccg ggcactctgt gcctcccatg agattcctta
      301 caccgtagta gaaacttcta tcgccaaaat cgtgtttgaa gaccgcaggg aaaataatcc
      361 ctgttctctc tgtgccaaaa tgcgtaaagg cgctctgaat gacgccataa aggatctggg
      421 ctgcaataag attgcctatg cccatcacga ggatgatgtt gtggagacca tgatgctctc
      481 cctcttttac gagggacgtc tgcatacctt ttccccggta acgcatctgg acaaaaccgg
      541 actgacggtg atccggccgc taatctatat gcgggaagcc gaggtcatcg gcttcgtgaa
      601 caaatatcag gttcccgtgg tcaaaagtcc ctgtcccgca gacggacata ccaagcggga
      661 atacatcaaa gaattattac aacagattca tcgggaaaat cccggtgtca gggatcgtat
      721 tttcaccgcg atccaacgag gaaatatgga aggttggtca gattactttg gcagtcatta
      781 acgaagaaaa ttatcacgat gaacagaata aaaacaatat cttaaaactc cgcaaggttc
      841 tggatacgct ccccagcttc tgcagagatt ttttccgtgg aattgagcct tatacttcca
      901 ccagaacaag actggcttat gcctatgata ttcgactttt ctttgaattt ctccatgaga
      961 aaaacagtta ttgccataaa atggagatta ctgaatttcc cctttccgtt ctggatatgg
     1021 tacagcggac ggatatcgaa gaatatctgg actatatgag cctgtatcag aaggatggga
     1081 aaaatattac gaacgaggaa cggggaaagg caagaaaact ggctgctctt cgaagcttct
     1141 ataattacta ttttcagagc gaacgtatcg agaaaaacac agcggcactg gttcctctgc
     1201 cgaagcgtca tgaaaaagag atcatccgtc tggaacccaa tgaggtggct attctgttag
     1261 atcaggtgga ggacggcacc aagctgacca aaaaggaact agaataccac aagaaaacaa
     1321 agctccggga tgttgccctc ctgacgcttc tgttgggaac cggcatccgt gtatcggaat
     1381 gtgtaggtct ggatattaac gacgtggatt tcaataacgg cggcatcaag atccggagaa
     1441 aaggtggtta tgaagcaatc gtctacttcg gcgatgaagt agaaaccgct ctgctcgact
     1501 atctggagca aagggaacat atcattccag ccgaaggaca tgagaatgct ctgttcctgt
     1561 ccctgcagag tcgccggatg gccgtccgct ccgtagagaa tctggtaaaa aaatatgcct
     1621 cccgggtaac caccttaaag aaaatcacac cccataaact acgcagtacc tacggtaccg
     1681 aactctataa agaaaccggg gatatctatc tggtagcgga tgttctgggt cataaagatg
     1741 taaataccac aagaaaacac tatgccgcac tggaagaaca acgccgtcgg caggccgcca
     1801 atgcttttca tctacgggaa cacagcaata gacattcgga ccagccggac aaatgacaat
     1861 tagaaaagat acccaacact tttggtgtat taggttaatc atctctgcat aaaatggaaa
     1921 gttagttaaa tacattggca aaatccacgg actcccggag catgcgatac acatgtctcg
     1981 atgtaaggtc tccggatgtg aattttctac gaaaagtgat gagtagttct cgtcatgacg
     2041 gggccgccta cagtcgacat ccatgtcgac aatcggctga cgcggacatc gtgtccgctt
     2101 tccccctgtc tttcagcact tttctagaaa atatcaacat cctccgacaa acatcttcga
     2161 catggcaccg catacgccgg gagttctgcg ttttggaaag ctttcaaaaa aaacagtagc
     2221 cccggctggg tataagaata agcagtgccg taagcagacg tcggtact
//
LOCUS       DAWDHD010000267         2189 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_3266_length_2189_cov_8.023899, whole genome shotgun sequence.
ACCESSION   DAWDHD010000267 DAWDHD010000000
VERSION     DAWDHD010000267.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3266_length_2189_cov_8.023899"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          609 a          483 c          670 g          427 t
ORIGIN      
        1 ccacggcact gggagcagcc tcccagaatg ctatggatta ccagccgccg ccgaacgaca
       61 gcggagagga aatgaacggt ctgacagatg ccatgcagcg tatcaacgat acttccaggc
      121 agatcagcga tattatcggt gagattgagg atatcgcatc ccagacgaat ctgttgtccc
      181 tgaatgcagc cattgaggca gccagagcag gagaggccgg aagaggcttt gcagtggttg
      241 cggatcagat ccgtaagctg gcagatgaca gtgcacagtc tgcggtacat accagagatt
      301 tgattgtgaa ttccctgcag gagattgagc atggcaacca gctgtcggac ttgacagccg
      361 aagcattgca gaaggtcgtg gaaggcattg aggatctggc caacgagtcc aagaaagcca
      421 tggaagaatc caaggcacag gcagatgcca tgatgcagat cgaacagggc attgaacaga
      481 tatccacagt ggtgcagaac aactctgcga ccgcagaaga aacttctgcc accagtgagg
      541 aattgtctgc gcaggccacc aacatgaacg agctgaccgc agcattccag ttaagagatc
      601 aaaatgtacg atgattcggt gtaatgaatt ctgaagatat gacatacaat gcttaataga
      661 aacaagcatt ggaggcagcc gtgttcgaaa aacagaatat acaggaaacc gtaaatgaac
      721 tgaaaagtga cgcagtgaag ggccttaccg atggtgaggc ctttcgtcgt ttacagcaaa
      781 atggcagaaa tgaaatgaaa gctgccagaa aaaagacgaa aatacagctt tttctggagc
      841 agttaaagga cccgctgatc tacatattat tgatcgcagc tgtggtatcc gtctttctgg
      901 gggagctcag tgatgccgtg atcatcggca ctgtggtagt ggtcaatgcc ctggtgggca
      961 tgctgcagga gggaaaagcc cgaaaggcac tggaagcgtt gaaaaagctg accagtccac
     1021 agactatagt gatccgggat ggaatacgca gagagattcc ggcggcggag ctggtgacag
     1081 gggatctggt agacctggag gcaggggcac aggtacctgc ggatatacgg ctgaccagaa
     1141 gctccaatct gcaggtagag gaatccgccc tgaccggaga atcggtacct gtggaaaaag
     1201 atgcgctgtt tctggcagac tgcagacacg agattccatt ggctgaacgg gtcaatatgg
     1261 tatatatgtc tactgtggtg acccatggcc acggagaagg aatcgtgaca gctacgggca
     1321 tggccacgga gatcggcagg atcgcctcca tgatcacaga tgcggaagag gaaatgacac
     1381 cgttacagaa acgtctgggg gatatgggaa agcttctgag tattttatcg ctgggagtat
     1441 gtgcggcatt gtttctgctg gcggtatttc aacacagaaa tattccggaa atgctgctgg
     1501 tggcgatttc cctggcggtg gctgcggtgc cggaaggtct gccggcagtg gtgacgatct
     1561 gtctggcatt gtcagtgacc agaatggtca aagtacatac gatcatccgc agactgcctt
     1621 ccgttgagac tctgggagcg gtcagcgtgg tctgctcgga taagaccggc acactgaccc
     1681 agaaccggct cacggtggaa aaatgctggt ggtatgatgt gctggaggat gctgccggca
     1741 gcaatggaag gggagaatcc cgatgcgtac aggagatgct ccggggaata ctgttgtgca
     1801 atgatgcttc cctgcaggga gagcaacgga tcggagatcc aacggaactg gcgctcttag
     1861 acttcggaga gaagatgggg atgcatagag aacgtctgga gaagcagtac ccccgctatg
     1921 atgaaaagcc tttcgattcg gaccggaaga tgatgacgac ctatcacaga atcccgaaaa
     1981 acggagggca taagggaagc attgcctata cgaagggggc accggatgtg atcgtacagc
     2041 actgcaccca tattttacag gacggaagaa gtgtgccaat gacagccgga cagagaaaaa
     2101 ggatcagtga ggttgtagaa ctcaggaatt ccctggcgct gcggccactg gcggcagcac
     2161 cgagcggaaa cacgcccggg tgtggggaa
//
LOCUS       DAWDHD010000268         2187 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_3271_length_2187_cov_7.726548, whole genome shotgun sequence.
ACCESSION   DAWDHD010000268 DAWDHD010000000
VERSION     DAWDHD010000268.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3271_length_2187_cov_7.726548"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          634 a          432 c          677 g          444 t
ORIGIN      
        1 gccttgatct gcctgagaaa atgtgtattt ttctatgaaa agagcggaac acagaatcat
       61 gcctggccgg aactgattgc ggaacaggca gaaatttatt ttttgctggg caatacggaa
      121 atggcggaac agttttaccg gagataccgt gagatgacgg gaagtgccgg agatcgggac
      181 cggaaccgta tgagggattt cgccagatgt ctggcctgca atgacaagac tacggaggga
      241 ttaaaagttc tggagaaggc atttgtcaat gtactggatg cggcaggaga aaagttggat
      301 ctgtgtgtct ggtgcgggga aaagacgatc gccggaaata ttctgacatc gtggccggag
      361 aagatcgaac tgattgggaa aaataccggg aatacccagg agtatttcga agattacttt
      421 ttccatctgg gatggtatgg gctgatctgt ggttccggaa aagtggccat caagaatatg
      481 gataaggcac tgatcttcca taaggaggat ctcagcaaaa aggatgacat tgcggacctg
      541 atcctggcat gcatcctgta cggagatgag aagaaaggtg ctgactatgc acaggcgttg
      601 aaagcctgta tggaaagaga agataaaagc ggcaaggatg tgtatctgaa atatccgaag
      661 cttcggatcg tacacgaata tctggcggga tattatacgg ccaccgatga ggaacaggat
      721 acgctcctgc aactggacaa agactgttcc ttctgccatg gctgtgtaca tcccgtctgc
      781 gaggaactgg agatggtacg gatactgcag atgctgaaaa agggccggga gaaagaagcg
      841 ctggagcggc tgaaagagca gatgcaggaa catcccggaa tgggattgca ggccatctgg
      901 caccggtatc attcggaaca ggtgacgaag gatacggatc ctgctgtagc cgcttttcac
      961 aaggaaaagc cgcagccgga gaaacgggga ttctggcaga ggctgttcgg gaagaagtaa
     1021 aaaattaaaa tgaagagaga agaaaacata tgatagtcgg aatcgatttg ggaaccacca
     1081 acagtctggt ggcagtattt acggaggaag gacctgtgat catccccaac cgtctgggaa
     1141 aacatctgac cccctctgtg gtcagcgtgg atgagaacgg caatgtctat gtgggggaga
     1201 cggcacagga acgcagaaac ctctatcccg acagtgtggc gcaggcattt aagcgcagca
     1261 tgggaacgga tcgcacttat gacatgtccg gcaaaaagtt ccgcccagaa gaattgtcca
     1321 gcatgatcct gcgttatttg aaagaagatg cggaagccta tctgggggaa gaagttacag
     1381 aggccgtgat cagtgtaccg gcctattttg acgacaagag aagaaaagcc accaagcggg
     1441 ccggagaact ggccgggctg aaggtggagc gtatgatctc ggagcctacc gcagcggcgg
     1501 tggcctacgg cctgtacgaa aaagaaaaag acaccagatt tctggtattt gacctgggtg
     1561 gcggaacttt tgatgtctcc attctggagc tgtatcacaa tattctggag gtgcgggcgg
     1621 tagccggaga caattatctt ggcggtgaag atttcacgga agtgatgtcg aaactttttc
     1681 tgcaaaagac aggactgcat tataaggatc tgtcggagaa agaacaggtg cgtctctata
     1741 aaaaggcaga agaagccaag cggggaatca gtgatcagac tgcggtgacc atggaactta
     1801 tgctgggtga agagaacaaa acggcagaga tcaccttaaa agaatatgag gaagagtgcg
     1861 aagaactgct aatgaagatc agggaaccgg tgaagaagag tctggcggat gcaggactga
     1921 agctgtcgga catcgatgag gtattgctga tcggcggagc caccagactg tcggtggtca
     1981 gggatttcct gatcaggctg ttccgcaaat tcccggatac aagactgaac ccggacgaag
     2041 ccgtggcact gggcgcggcg atccaggcgg ccatgaagga acgccgggcg gaggtaaagg
     2101 aagtcattct gacggatgta tgctccttta cactgggaac ggaagtcgtg gtggaatacg
     2161 aggaaggcaa atttgaagac ggccgtt
//
LOCUS       DAWDHD010000269         2174 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_3296_length_2174_cov_9.800378, whole genome shotgun sequence.
ACCESSION   DAWDHD010000269 DAWDHD010000000
VERSION     DAWDHD010000269.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3296_length_2174_cov_9.800378"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          625 a          414 c          633 g          502 t
ORIGIN      
        1 ttcgcaccgg agcattttgc cagagtgcgt caggtggata ccatgaagag aaaacagttc
       61 gtcaagggcg tagagcagat cgctcaggaa ggtgccatcc agatctttca ggagttcaat
      121 accggtctgg aagagatcat cgtcggtgtg gtaggtgtgc tgcagttcga tgtactgaaa
      181 taccgtctgg agaacgaata taacgtggag atccgtctgg agaacctgcc ttatgagcat
      241 atccgctgga tcgagaataa ggatgagatc gacctggcca agctgtccgg tacttccgat
      301 atgaagaagg tcaaggacat gaagggtaat cctttattgc tgtttgtgaa ttcctggagc
      361 atcggcatga ccctggaccg taataaggga ctggtgctga cggagttcgg acgcaattag
      421 taattatatg gaaacaaaaa gtgtgtaggg ggatagaatg cggaataaga aaaagttatg
      481 tgccggaatt ctgatgttac tgcttttgac aggatgctcc tcacaggagg ttctgcagga
      541 agtaccccag accatcgtgc tgcccgagtc acagatagat actatgcctg tgcaggagac
      601 ggcggctgcg gaaaatgaca atacagagag cttttccctt ttagaggagg gaggctctcg
      661 ttttgcatat gagagtctgg atgcgcagga acagatctgg tatcaggaga tcgagcaggc
      721 gattgggtct atggatgaca cgatcaaact gtccacggaa ccgttggaac agggaatgga
      781 tgaacaggac attgacaaag tatttcagtg cgtgctgata gatcatccgg agatttttta
      841 caccacagga tatacttata ccaaatattc cagaggggag aaaaccgtag gaatcgactt
      901 cgcaggcact tacgatcaga cgaaggaaga ggccgtagcg cgggcggagg ccattcgcag
      961 tgtgacggcg gaatggctgt ccggtatgga agaggcggct tctgagtacg ataaggtaaa
     1021 agcggtgtat gaggcggtca tcttttcaac aaattatgat ctgagtgcgg cggataatca
     1081 gaacattgcc tcggtattct taggaggagc ctctgtctgc cagggatatg ccaaagccac
     1141 ccagtatctg ttgaatcatc tgggggtgat gtgcaccctg gtgcagggaa cggtggatac
     1201 cggagaagct catgcctgga atctggtaca ggtggacggg gactattatt atgtagatac
     1261 cacctgggga gatgcctctt accgcatgga gaatggcagc gagcagagga gcctgccgga
     1321 gatcaattat gattatctgt gcgtgactac agaggatctg ctacggacgc atcgtatcga
     1381 agcggtgatc cccatgccgg aatgtacggc gatggaagca aattattatg tgcgggaagg
     1441 ttcctacttt acttcctatg accatgaaca gatgcaggcg gtcttcgaca gagccataga
     1501 gggcggcaga acggaagtga cgataaaatg tgcagatgaa ctctgttatc aggaaatctg
     1561 tcatacattg atcgatgagc aggagatttt ttcctatatg cagatacagg ggaacagcaa
     1621 taccgtcact tatgcccaga acgggaaaca attatccttg actttttggg tgacaaatga
     1681 ataaggattt ggtatactgt aactaattag ttattattag gaggatcagc tatcatggaa
     1741 aaagaaatag ataagaagaa cctggtatta agggaagacg agagcatcgg taccgtacag
     1801 atcgccgatg atgtagtggc tatgatcgca tctttagcag ctaccgaggt agacggtgtc
     1861 agcgccatgg ctggtaatgt taccaacgaa ctcatgagca aggtaggcgt taagagcctg
     1921 accaagggtg tcaaagtaga ggtgctggac aagaatgtga ccgtggatct gactgtgacc
     1981 atggagtacg gttacaatat tcccgctacc tgtcaggcag tacagcagaa ggtgaaaaat
     2041 gccattgaga atatgacagg actgacctgc tctgatgtga acatccgtat tgccggtgtg
     2101 aacatgaaga aggataaata agaggaatta caggaagata ttatgggaag acacgagcag
     2161 agagaacagg tttt
//
LOCUS       DAWDHD010000270         2159 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_3322_length_2159_cov_9.189639, whole genome shotgun sequence.
ACCESSION   DAWDHD010000270 DAWDHD010000000
VERSION     DAWDHD010000270.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3322_length_2159_cov_9.189639"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          582 a          462 c          514 g          601 t
ORIGIN      
        1 aagtcatttt tgttttattg ttttgtgtat ttttatcctg tgacttttgt attcctttcg
       61 ttattcccat tcataattct caaaagagcg gaagtgccgg tgcggttcgt ggatctcaaa
      121 agagcggaag tgccggtgca gttcgtggac ctcaaaagag cggaagtgcc ggtgcggttc
      181 gtggatctca aaagctgcgg gagtgccaga actgtccgtg tgcctcaggg actgcggaac
      241 cgtcagagtt attcgtgatt ctcagaggtc tcggaactgt gttccacatg ttcttcgttg
      301 ggttctggaa tcgtcatcag taccttgatg atacggttct ggtcgatacc ctgtaccttc
      361 aagtgaatac cgttctccag ggtaacttcc tcaccatcct ccggcagacg atccagacag
      421 tcgatgatca gaccgccgat ggagtcataa tcttcactgg tcagatttgt tccaagaacg
      481 tcgttgatgt catccagctt catgctgcct tccaccagat aagtatcttc tgacatcttc
      541 tggatcagtt cttcctcgtc ctcatcgtat tcgtcacgga tctcaccgac gatctcttct
      601 aacagatcct ccagagtgat cataccaacg gtagcaccgt attcattcaa tacgaaagag
      661 acattggtgg tcttttcccg catctcatac atgaggtctg cgattttctt gtactcgtaa
      721 gtatagtaag catcacgcaa aatgtttccc acatggaaag cactctgatc ctccgtcagg
      781 ataaagtcct tgatattgat cagaccgata atattgtcct tgtcatcctc atagaccgga
      841 agcctggtat acatggaatc ccggaaaact gccagaacat cgtcataagt agcatccaga
      901 ctgaccgtta ccatattgat gcgtggaatc atgatatcct tggcgtgggc atcggaaaaa
      961 tcgaacacat tgtagatcag cttgcgttcc tcggattcga tgacaccgtc ctcatgactg
     1021 acgtccacgt aggttttcag ctcgctttca gtcatggtgc tggctttttt gttgggatcg
     1081 atatgtaaca gcttcatgat accgtgggac agcagatcca caatgaagat gaccggcgtg
     1141 agcacctgca tcagaaaata gattacattg caatatgtca gggccagctt ctcagaatac
     1201 agtattgccc aggtcttggg tacgatttcg ccgcagagca gcacgacaat ggtcagaaca
     1261 ccggttgcga tacctacagc cagattgatt cgcatagcta atgttgttgc cagggaagat
     1321 gcagtcagat tgacaatatt gttgccgatc agaatggtac tgatcatttt actgtaattg
     1381 tccaggactt tgttgacgcg tgcagcacgc ttgctgcctt cctcttccag ggtacgcatc
     1441 cgtacacggt tgaccgtgga cagcgcagtc tcggcagaag agaaaaagcc ggatagaaaa
     1501 atcagaatta cgatagaaac aagttgtatg acaccagagg tatccaaaaa agttcactcc
     1561 ttcttaaata atagtagtag gcataagccc catttataag ttattaagca acaatataca
     1621 ataggacagg aatagttgtc aagttttccg aatgatttga atatacattt tacataggta
     1681 aaaaggagga gcaacatgga aaaaagaggc gagagaaaag aaggggttcc ggtgcttgca
     1741 ctggcaaaat gtctgctggc atcctatgtg ctgacactgt tgcttttatg tctgttggca
     1801 cttttattgt ataaattgca tctgacggaa ggaatggtga ccattgcaat ctctgctatt
     1861 tatgtcataa ctacctttat ggcgggattt ttggcaggaa aaaagatgga gacgcagaag
     1921 tttctttggg gagcactgct aggggcagta tatttcgtga tactggcggt ggtatccgta
     1981 ttgatcaaaa cgccggaaac tgcattgggc aattccttct ggaccacaat ggtattgtgt
     2041 gtggccgggg ggacactggg cggaatgtta gggtaaaata cagaaatgga caggaattta
     2101 acggtttttg agaaaaaaag cttttacaaa tgccaaatac atgatataat gagcaaaaa
//
LOCUS       DAWDHD010000271         2107 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_3440_length_2107_cov_7.522904, whole genome shotgun sequence.
ACCESSION   DAWDHD010000271 DAWDHD010000000
VERSION     DAWDHD010000271.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3440_length_2107_cov_7.522904"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          467 a          591 c          374 g          675 t
ORIGIN      
        1 gcatctacca gctcatccat cttctgctgg ctgttggctg cctcgatacc tacctgtttt
       61 gccttatcgt gaacgatatc ggtagacttg gtgttctcca ttacctgatt cgttacttct
      121 tctacggtag ccaccagttc ttcaacagct gcagcctgac tggtagcacc ttctgcaata
      181 tcctgtgcac tgacagccag ctgattgctt ccggcagaca cctgttcgga agatccctga
      241 ataccgtgca tagtctcact gaccttgttc accatatcat ccagttcatc cagtacattg
      301 cggagctgtc ctacatacgc atcggggcag ttgctatgta catcaaaatt accctcagca
      361 aaatgctcta caatatcctg cagatctgat acaacctttt tcaaaatacc ggctgcggtc
      421 tcaaaactgt cggataagcg tcccagttca tcctcggact cataaggagt gccgatctca
      481 aagtcaccgg aagcaatctt ctcagatgcc actaccagac tctcaatagg ctctaccaac
      541 attttggcaa gcatctttga catagctgca ttaaatgcaa tagctaatac aactatgatc
      601 acaacaatta cgcttcccac atagtttttt gactgtatca atcccacaac tgccaccagt
      661 gccagcacca catataaaac gtccataatg cggaacacaa aagaaagctt atttttaatc
      721 ggtttctccc ttagcatttc ttccagtttt ttcatttgat tccttctgct tccgttaatt
      781 aacctagata ctttttattt taaattcctc tggttgtttc gtcaagattt ctctgcttaa
      841 aattatgtaa aaatcatgta tgggatgact ggctcatctc cagcagtttc tcacgcattt
      901 cattttcgat ccgctgtaag tcttcctctg cgctctgtct tctggcacgg ccttcctcct
      961 ggatcttcat gacttcatcc agtgtggtca tcagtgtctg gttcgtggtc ttgagggtct
     1021 cgatatccac aatacctctc tctgattcct tcgctgtctc cacggttgcc actttcagtg
     1081 cttctgcatt tttcttcaac agttcattgg tcatatccga tactgcacgc tgtgccttgg
     1141 ctgcatcggt ggcatgatcc agaccgatgg caatgaccat ctggctcttc cacagaggga
     1201 tcgtattcac cagtgtggac tggatcttct cggacatagc gatatcattg gactggatca
     1261 gtcggatctg gggtgccatc tgcagagaaa tggctctggt aagttccaga tcatagatct
     1321 tcttctcgaa tctctcacac attgctgcaa aatccttcgc agcctgcgta tcctccggca
     1381 gtccgctctt ctgtgctttt tccagaagtt ccggcagttc cacgtttttc gcctgtgtca
     1441 gtttcttttt acctgccaga atgtacatgg acagttcttt aaaataattc agattcagct
     1501 gatacagctt gtccagtacc gccacatctt ttagcagcac cacctgatga ttttccatgg
     1561 catcacagat cttgctaatg ttcatttccg ctttatcata ctttgctttc agattattca
     1621 gcttatcccc gctcttttta aacaagccga agaatccctt gtcttcctct gtatcaaaaa
     1681 ccttgagctg tgccactaca tccgtcaaca gctgtccaac ctcgcccata tccttggttc
     1741 ggacattgct aagtgctgtc tcagagaaat ctgcgatctt tttctgactc cccgcaccgt
     1801 actgcaatac cgcctgggta ttggtcagat cgatctgtcc cgcaaaatca tctaccattt
     1861 tctgttcttc cggtgtcagt tccatctccg gaacagcctg ctcctctacc agttcctccg
     1921 gcttcttctc cacgatctct gcctttgcct ccgcataggg atccagtgta agggtaggtg
     1981 cctcttttgt tgctgcatcc agatccatat tcatacttat aaccccgcct ttccatactc
     2041 tctcatgatt ttttaattcc catcagcatt actttcctgc tgtcatacac ttttttctct
     2101 tacaaaa
//
LOCUS       DAWDHD010000272         2003 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_3736_length_2003_cov_14.160678, whole genome shotgun sequence.
ACCESSION   DAWDHD010000272 DAWDHD010000000
VERSION     DAWDHD010000272.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3736_length_2003_cov_14.160678"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          539 a          462 c          484 g          518 t
ORIGIN      
        1 actgcggcta tgactgcatc gaggtggtca acggcttccg gggtgatcat ttcggattcg
       61 aggatgtggc ttattttctt ctgttcggta agctgccgaa ccgtgaggaa ctgaaacatt
      121 ttaacgacat tcttgcgtcc agcagaactc tgccgaccaa cttcaccaga gacgtcatta
      181 tgaaagcccc cagcagtgat attatgaatt cactgaccag gagcgttctg acactggctt
      241 cctatgataa aaactgcagc gacacttcca ttgaaaatgt attaagacag tgcctgggac
      301 tgattgctgt gttccccatg ctggcagtct acggctacca tgcatataat cactattcca
      361 acgatgagag tatgtacata caccgtcccc agaagaaact ttccaccgca gagaatctgc
      421 tgatgatgct tcgcccggac aaacaataca ccgaactgga ggcgagggta ctggacaccg
      481 cactggtgtt gcatatggag catggcggtg gtaacaactc cacctttacc accagagtcg
      541 tgacttcttc cggatccgat acttattctg tcattgcggc agcactttcc tccctgaagg
      601 gacccaaaca tggtggtgcc aatatcaagg tcgtagagat gatgcgggat atcgaagcac
      661 atgtgtccga ctggacagat gaggatgcgg taagggcata tctgaacaag attctgagca
      721 aagaagcctt cgacggcaag ggactgatct atggtatggg gcatgcagtc tactccctgt
      781 ctgaccccag agcccaggta ttcaagagct ttgtggaaaa actggcggta gccaagggca
      841 gagacaagga tttcgccctg tattccatga tcgagcggat cgcaccggag gtcatttccc
      901 agaaatcccg gatctacaag ggtgtcagcg ccaacgttga tttctacagc ggtttcgtat
      961 acagcatgtt ggagattcct ctggaactgt ataccccgat cttcgccatt gccagaatcg
     1021 taggatggag tgcacatcgt atcgaagagc tgatcaatat ggataagatc attcgtcccg
     1081 cttacaagag cgtaatgcag gaactggaat atgttccttt ggatcagaga taaattatca
     1141 aataataaag gactgcgtat tttgcgcagt cctttgctga attactatgg agaacagatc
     1201 ggggaaccta cgaataaatc ttgcggataa tggatacgat ctcttcagcg gattctttgg
     1261 aatcctccag aatccaggtg acataaatat tgtagaggga accggagaag gctcggagcg
     1321 tgtaataaaa atcccgcccc ttgtcttcgg tataataggt atccagaatg tagctgtcca
     1381 gtgcctgtaa cagcaggtgc cccagaccgg tctgcaccag acaggcaatg aattctttgt
     1441 aatgattgaa atattcataa caatgcagca tattgcggga gtcattgtaa ttgcccttgt
     1501 ccggccatgt cgcaacatcc tgacggtagg attctaccag atccttcaga taactgctga
     1561 agatctcatc cagagaatgg taattgcgat aataggtcat gcgggagaca ccggcacgct
     1621 cggtcagctc ggtaattgta atattggaca gggatttggt cttcagcagc tggatcagag
     1681 cctgtaccat acaatctctg gaaaaaaagt tggaattctt tgtggacatg acggatcacc
     1741 ttctttatga tttctaccta ataatgtaac gattcagacc tccattcgca aaatcgcctc
     1801 tatgaggctt ccttggagaa caggccggcc tgttctctgc tcattgtggt tacttcgcca
     1861 tataaatcat atcaaatgtt cttacgaatg caagcattcg acgtacatgt tgacatgatt
     1921 tgcatggctc tgaatagtta ccatataatt atatgatgtc ggggtcaaaa gcccccgact
     1981 ttgatgccta cggattgttc cgt
//
LOCUS       DAWDHD010000273         1977 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            k141_25424_length_1977_cov_13.0000, whole genome shotgun sequence.
ACCESSION   DAWDHD010000273 DAWDHD010000000
VERSION     DAWDHD010000273.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25424_length_1977_cov_13.0000"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          634 a          350 c          582 g          411 t
ORIGIN      
        1 agaaactaaa agaaaaaaga aaaaaaacct cagtctggaa tattttttgt cggtgaaact
       61 ttttgcggga atacagcgtc aaaatcgtag aatatatcaa aagaatatgt gttacaataa
      121 ataccgaaaa ataattgcat gcagttttca aaagtgagga aaggatatat gtcggacaga
      181 aaaaaagaac aggcggtgga gcgtgccctg acggaagggt atgagaagta ttaccgtctt
      241 gcttatagtt atgtacataa tgaagcggac gcgctggata ttgtacagga agcagcctat
      301 aaagctattt taaaaagtga cagcctgaag gagtcgcaat acgtggagac ctgggtgtac
      361 cggatcgtga tcaacgaggc gtgcagcttc ctgcgcagcc ggaaggagag cgtggatgtg
      421 gaggagatcc aggcggcaag tgaagatatc tatgaaaata ttgatttaaa gcgcgcaata
      481 gaaaatcttg acccaaagga tcgtgccatc gtagtcctac gattttttga ggacaggcag
      541 ctggaggaga tcgcgaagat cctggatgag aacctgagta cggtaaagag caggctgtac
      601 cgggtgatga aaaaactgcg gctgaacctc gagggcagca tgggctgagc aatccgtagt
      661 caaagtcgtg ggattttgcc ccgacatcat atataggcag gacataagac agatacagac
      721 aaagagatat aggaagatat aggaagagat aacatgaccg gtccgaaccg accgggcaga
      781 tggagaatag atatgacaga acaggaacag ttgagacaat taaaagagga gtatgagaat
      841 atgataatac ccgaagcggg acgggaacgg ctgcaggcag gtatcgacag agcccggatg
      901 gagaaaaaaa gagtagagca cgccagaagg cgttctgcat ggacagcagt ggcggctgca
      961 gcagtcgtga tgatcgcact gccaaatacc aatatacaga tagcccatgc catggaaaat
     1021 attccgttgc tgggtggctt ttttaggctg gtaacagtgc ggcagtacaa ttacagtgac
     1081 gagaaccatg atgctgaagt agaacttgca cagatcaact atggagaaga cgcaggagaa
     1141 ggtgcttccg ttggagaagt ggcagccact cctaagggca cagcagccgg aagtgtggaa
     1201 ggtgtcggac aggaggctgc ggtagcaaat ctgtcggaag acggtgtgga agcggtcaac
     1261 caggatatgg aggctacggt agaggaactg atccgtcagt tcgaggatac cttatccgag
     1321 gaaggatacc atggtctgca tgtgtcccag gaggtcgtta cggacaatga gaggtattac
     1381 accgtaaaat taagcgtgtt ggagacggaa gccagcggct acgagaataa tcagttttat
     1441 acgattgata aacagacggg aaatgtggtg acactggaag atctgtttgt ggaagggagc
     1501 gactatattt ccgcgatcag cgagaacatt aagacccaga tgcaggagca gatggcggcg
     1561 gacgaaggcg tgatctattt cctggataat gacgatatgc cggaattcaa ttttcagggg
     1621 atcacggagc agacgaactt ctattttaat gaaaaagacg aactggtcat tgcctttgac
     1681 gaatacgaag tggctcccgg ttctatggga gcaccggaat ttgtgattcc gcaggaagtg
     1741 acggcagcaa ttttaaagta agcaaaaaat atctaaatgg ggaaactccc gcatatcatg
     1801 aaacaaaagg atatgcggga gtttttttat gtcattgatt gtattggatg cggggcatgg
     1861 tggtgcgaac cccggggcca cctataatgg aagaaaagaa agtgaggatg cgctggcact
     1921 gacactggcg gtgggaagca tcttagaaga aaacggagtg gatgtgtatt acaccag
//
LOCUS       DAWDHD010000274         1967 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_3839_length_1967_cov_8.650628, whole genome shotgun sequence.
ACCESSION   DAWDHD010000274 DAWDHD010000000
VERSION     DAWDHD010000274.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1967)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3839_length_1967_cov_8.650628"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          518 a          376 c          590 g          483 t
ORIGIN      
        1 tgtggctggt atgtgtggca atgggaattg cgggaattcc tgcaatctga cgaaccgcag
       61 gatgctctgt aaatagtaaa gtggaagacg gggaagacag tgacatggag gatatggcgt
      121 atatggaaga gaaaaaatgg gagacagggg acagcgaaga gttgatccgt gtacaggaga
      181 tcagcaagac atttcatctg tctgcaaaac aacgaaaaat gaagcacagt gcatcgaaga
      241 ccaaaacagc ggtggatcaa gtgagctttt gcgtaaaaag gggggaagtg ttcggacttt
      301 tgggacctaa cggtgccggt aagaccacaa cgcttcggat gctggcaacg ctgatccggc
      361 cggacagcgg ggatgcggtg ttatccggca gcagcgtagt gacacagccg gaggaggtac
      421 ggcgcaagat cggcttcctt accagtgaac tgaagctgga ggattttttt accccggatt
      481 atctgtatga ctttttctct gaattgtacg gtattccgac agacagacgg gacgccagaa
      541 aacgcatgct gtttgcacgg ttcggcattc aggagtttgc acaggtgcgc gttagggatt
      601 tgtccacggg aatgaagcag aaggtatccc tggtgatctc actggtgcag gatccggaga
      661 tcataatttt cgatgagccc accaatgggc tggatattct cacagcgaag gtggtgacgg
      721 atttcctgct ggatctgaag gagcagggga agaccatcat tgtatccacc catatcttta
      781 gtctggtgga gaagatctgt gaccgtgtgg ggattattat taatgggcag atgacggttt
      841 gtgaaaatct gcagacactc tgtggagaga agagtctgga ggatcgtttt tttgagatct
      901 atgaagcggt aagtggggag gaaacggacc atgacaaata atacgatcac agtaatgaaa
      961 aaagagctgg ccagattttt cggagacaga agactggtga tcaccacact gctgcttccc
     1021 ggaattatga tttatgtggt gtacagcttt ttgggcagtg tcatgatgaa gaccatgctt
     1081 ccggaagata cttatgtggc gaaggcttat gtggtggaca tgccggattc cgtaagagaa
     1141 gagatgcggg aactgcgggt ggactggcag caggcggact gagagcagct gacggagata
     1201 gggcaggaga tcaaggaaaa aaaggtggag ggtctggtgg tatttccggc ggattttgat
     1261 acaggggtgg aaaactacca ggtcagtagt ggggaacctg cgcccaatgt agaaatttat
     1321 tacaattccg cggaaccgga atccactcat ttttacaatg aggtatcgga ggtgctggag
     1381 cagtatgaga cttccttagc taataaactg gatatcaatg ccggagattc ggtatactat
     1441 gactgtgcca ccagcaaaga tactacgggg cagatgtttt ccatgatgat gccgttgttg
     1501 cttatgatgt ttttgtacag tggatgtatg tctgtggcac cggagtccat tgctggggaa
     1561 aaagagcggg gtaccatagc gacattactg gtgacaccca tgaagagaag ttccctggct
     1621 ctgggaaaag tgttttcctt aagtatcata gcgcttctgg caggctgttc cagttttatc
     1681 ggaacttttg cggcattgcc gaaaatgatg ggcggagaac tgaccggcgt ggattcctcg
     1741 gtgtacgtac ctatggatta tgcgatgctg cttctgatca ttctgtcaac ggtcatggtg
     1801 ttggtatcca tgatcgcact ggtctccgct tttgcaaaaa gcgtgaaaga agcggctacg
     1861 acagtctctc catttaccat cgtggtgaca tttatcggac tgagccctat gctcagtcag
     1921 ggaaaagaga ttccgctgta ccggtatctg attccggtat ataacag
//
LOCUS       DAWDHD010000275         1946 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_3912_length_1946_cov_10.024855, whole genome shotgun sequence.
ACCESSION   DAWDHD010000275 DAWDHD010000000
VERSION     DAWDHD010000275.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1946)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3912_length_1946_cov_10.024855"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          539 a          417 c          543 g          447 t
ORIGIN      
        1 tggcgctgtg ctggcagatc tttttcgcag gccccggagt gcggaatgtc tttggatttg
       61 ttgtgggatt tatattggat agaaaaagta taattgacta tcatctgtga gtgtgctaac
      121 atgagcaaat aaaaagacaa cgaagtccgt ggaaacggaa agcgttgtct cttttatttt
      181 gtagaaaaag tcaggaaatt acgaggtgaa acttatgagt gagcaggaaa agaagcaaaa
      241 tacagttgca gaggtgttag taaaatgtct ggaaaacgag ggcgtgaaat atgtcttcgg
      301 gattccgggg gaggaaaatc tggatatgat gaatgcactg gaaaaatcgg acatccgggt
      361 gatcgtggtg cggcatgaac agggagcggc attcatggct gatatgtacg gaagactgac
      421 gggaaaagca ggtgtgtgtt tctcaacgtt gggccccggt gccacgaacc tgatcacagg
      481 cacggcagat gctaattccg acggtgcacc gctgattgcc attaccggac aggtgggaac
      541 ggagcggatg catttgactt ctcatcagta tctggatctg gtagagctgt ttgcaccaat
      601 caccaagcgg agcaaacaga tcgtgaaccc caattccgtc aatgaaatcg tcaggatcgc
      661 ttttaaatat gcggagagtg agaagccggg agcctgtcat gtggatcttc ccacgaacgt
      721 ggcgaagctt ccggtcagct ccggtattgc cgcacagcct ttgaaaaagc cggagaggga
      781 taaggagtac gcatcctttt ccagtatcga tcaggcggcg gccatgatct tcaaggcaaa
      841 gcacccggtg atcctggtgg gacacagtgc ggtgcgcaat cacgccgggg aggctcttac
      901 cagcttcgca gatgtcctga agatcccggt ggtcaatact atgatggcca aaggcatcat
      961 tccttataac aacaaatatt ccctctggac cataggcatc ccccagaagg attaccagaa
     1021 caaggtgctg gatatggcag atctggtgat cgccgtcggt tatgatattg tggaatttgc
     1081 tcccggcaaa tggaacggag aggggaaaca taagattatt catatcgacc agaggccggc
     1141 gcatatcaac atgctgtacc agccggaggt ggaagttgtc ggagacattt cctattcttt
     1201 acagcagatc ctgtaccgca gtgacgcaaa agaggagccg gaagaatttc tcaaactgcg
     1261 ggaagaaatg gtggcggaat atgaaagcta tgcagatgac acctcttttc ccatgaagcc
     1321 acagaaaata ctgtacgatg tccgtaaatt tatgggtgcg gatgacatcg tgatctcgga
     1381 tgtgggcgct cataaaatgt ggattgccag agagtacaac tgttacgaac ccaatacctg
     1441 catcatctcc aacggttttg ccaccatggg catcgcagtg ccgggggctg tggcagccaa
     1501 gttgatctac ccggagaaaa aggtactggc gatctccgga gacggcggat tcatgatgaa
     1561 tagccaggag tatgagacag ccctcagaga ggggacaccc attgtgactc tgatcttctc
     1621 cgatgccagc tatggtctga tcaaatggaa gcagatggat cacttcggac acaactgctt
     1681 tgtggatttc cagaatcccg attttgtgaa atatgcggaa agcatgcatg ccaaaggcta
     1741 ccgcgtggag aaggcggaag acctgctgcc gattctggag gatgcctttc agcagaaggt
     1801 cccctgcatc atcgactgtc ctgtggacta cagcgaaaat accaagctct cggagtatct
     1861 gcatgataag tttgagaagt agagttttaa ctagaaagtt agttaagaaa tgctatcaaa
     1921 tccccgtagc ccttggcaag gtataa
//
LOCUS       DAWDHD010000276         1893 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_4085_length_1893_cov_8.187160, whole genome shotgun sequence.
ACCESSION   DAWDHD010000276 DAWDHD010000000
VERSION     DAWDHD010000276.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1893)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4085_length_1893_cov_8.187160"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          450 a          543 c          360 g          540 t
ORIGIN      
        1 gatcgtagag gtttgtaatt ttcttggcat gcatctcacc gatggttccg ttcagtacgg
       61 aagcatcctg gctatataca tatacaagat taccattgat cactccatag ccggtaatac
      121 aaccgtcaga aggagtttca ttgggtttca gattgaaatc cgtagctctt gcggttacaa
      181 gtccgccgat ttcaacgaaa ctgttgtcat cgagtaaagc tgctattctt tgactcgctt
      241 ttgaagtagt actcatatgt tttcagccct ccgttgataa tataataaaa tataatagaa
      301 aacgtttatt aatatagaag cacaaatgcc cccatgatta acagtttgta gtataacata
      361 aaatctgcaa ctcgcaaaga acgaattgca gatttttaca ttttttatat attgtacctc
      421 attttgaaca atttccccgt cattttttcc attccatttt tctgcaatcc ctacgggaat
      481 gctaccagaa aacgtttcag gatctgtctg ccgcaccagg aaagatccac ggcctccact
      541 gtccaagcag catacagccg ctccctgcgc cagatttcgc catccaccag ataacagatt
      601 tctcccaccg tcgttccttc ctttaccggt gccttcagtt ctttctcttt ccggtagaga
      661 atctccactt tctcgtgtgc gcgcataagc aggccttccg ctccttccgc ctccggatcc
      721 agttccaccg ccagctgcac ttctcctgtc agttcctcat cctgtgcctc cgtcaccggg
      781 atcggcataa ggtactgttc ccgtccggaa aattcttcct ctccgaactt ccggtaaaca
      841 aaattttcca gtccatatcc aaacaactct ctgctgtctc tccatttata ggtcttatga
      901 ttcggccacc cacaggccag aagtgccaca atcatacatt tcccatcccg ttccagtgct
      961 cccacatagc agtaccccgc cttgttcgta aatccggtct tgccggtaaa agctccgtcc
     1021 atcatctgca aaaaggcatt gtggttgtgg cactgaaagg agcgtccgtt ttccgtgaaa
     1081 caatactcct gtgtccgggt gatctcccgg aacagatctc tctgggaaga ctctctgata
     1141 cagtaccgta aaatacaggc cagatccttt gctgtggtat ggtgctcctt ttccagaata
     1201 ctcccgtcag gtaatgtcaa tgtctctgtg gcatccagcc cgttgggagt gataaaccag
     1261 gtatcctcgc agccgatctc caccgccttt tcattcatcg cctgtgcaaa tctatgtaat
     1321 gctgccttac tctcttctgt agtatgctcc gaagcctctt tcgtctcacc cagaagcttt
     1381 ccgccgatat attccgccag tgccgcagca gcatcattat ggctttccag catcagggaa
     1441 aacagcagtt ctcttaccgt ataatgctcc ccctttttca catagagctt taccttgggc
     1501 atagtggctg cataggcgga aatactgacc tcttcctcca cctttgcatt ttccagcacc
     1561 agaatgcagg tcatgatctt ggtggtgctg gccattgcca tgggagtctc ttcatttttt
     1621 ccatatagta cccgcccgga gtcggcatcc agaagcaccg ccgcctgtgc atacaggtgt
     1681 aaatcctcct gcagctgccc ggcttccttt tcctctgttt catgaaaaag gtgcgtagcc
     1741 tgttcccgga tttcctcgct gacatacccc tgccgacaga atccgcctat actttccaga
     1801 cacagaattc cggcgaaaag ggcacaaaaa aagcctcgca taatcatcac tcccaaaaga
     1861 gacgcttatt acctgtcgct taaacacatc aga
//
LOCUS       DAWDHD010000277         1819 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_4341_length_1819_cov_9.438776, whole genome shotgun sequence.
ACCESSION   DAWDHD010000277 DAWDHD010000000
VERSION     DAWDHD010000277.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4341_length_1819_cov_9.438776"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          492 a          381 c          441 g          505 t
ORIGIN      
        1 tttttttaag tttcggcctc caccgttttc ttcacttttg cgtttgtggg catgggcagt
       61 agtgtaatag agacagcatc gcttctctga aaaatatttc cgaccggccc atgaatctca
      121 agggtgagga actgtcagag agatttatcc ggggagatgc ttcccgtact accgagggaa
      181 gtggtctggg tctgtatatt gcgaaaaatc tgaccaaggc acagcacgga gaattccaga
      241 ttcagctgga tggagatctg tttaagatta tattggattt cccggaatac cataagccgg
      301 aagaacccgc cactgagcag ctgaaggaga ccgtgtcgaa agaggtttag aaatggcgaa
      361 ataagaaata ctgaaataag caatataact gaaaaacgca gaaaccataa cggcttctgc
      421 gtttttttat gcgttgtcag atctgttgga aattaaaaat gaaattcgct catttccgta
      481 cgattgttgt aggcttctac aatatcgtgg atcttttcgg tggtagccat gacattggac
      541 agagaagcgt catggttcat aaagtcgatc agggaagcaa tgaacttgct catatcggct
      601 tccacgaaat aaggtctggt gcagatctca ggcggcagat aggtaaggtt cgtagtaatg
      661 accttatcga aatcaccatg ctcgtaagca gcgtcgaaat tcttcagacc gtcggtgaac
      721 aagccgaaag tacagcagat atatacgcgt ctggctttca tggcctttaa ctgttttgca
      781 gtatccagca tacttccacc ggaggagatc atgtcatcga tgatgatgat atccttacct
      841 tcaatattgt cacctaagaa ttcgtgagca acgatagggt tcttgccgtt gacgatggtg
      901 gaatagtcac gtctcttgta gaacatacca gtatcgacac ccagaacgct ggcaaaataa
      961 atggcacgat ccagagctcc ttcatccggg ctgatgacta acagatgatc cttgtccacg
     1021 atcatatcgt cttcggaatt taacaaggac ttgatgaact ggtaaggggt ggtaaaatta
     1081 tcaaatccgt tcagcggagt ggagttctgt actctgggat catgggcatc aaaagtgatg
     1141 aagttatcaa ttcccatatc ccgtaactct tccagtgcat aagcacagtc cagggattct
     1201 ctgccgctgc ggcgatgctg tctgccttca tagaggaaag gcatgatgac gttgatacgt
     1261 ctggccttgc cgttgcaggc ggcgatgaca cgcttcagat cctggtagtg gtcatcagga
     1321 gacatgtggt tggtatagcc gttcatgttg taagtaatac tatgattgca tacatcggtg
     1381 agaatgaaca gatccttacc gcggatggtc tcgctgatct gcgctttggc ttcaccgctt
     1441 ccgaaacggg gcacatccac gtctacgaga tagttgctct cgatatagcc gtgaaatgca
     1501 ggatcgtttt tcactttgtc attatggata ctcttacgga attctaccat gtagtcattg
     1561 atgcggcggc ccattttttc tgcggaaggc agagcgatga tcttcaaagg agccacgggc
     1621 atggcatttt ccagttcgtg taaattaggc atgatgtccc tcccggacga gctttattgt
     1681 tcgtaaaaat atgcagatct tccttcgcaa aagcgcatct tcgatgctta attttgcgga
     1741 actattctga atataacaat taatttataa cattatgcta gtgacacgtc aaggaatttc
     1801 ttgtacaatg gataggaaa
//
LOCUS       DAWDHD010000278         1650 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_5012_length_1650_cov_10.657680, whole genome shotgun sequence.
ACCESSION   DAWDHD010000278 DAWDHD010000000
VERSION     DAWDHD010000278.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5012_length_1650_cov_10.657680"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          404 a          333 c          477 g          436 t
ORIGIN      
        1 tggcattaca caccggaaga gacaagaaaa gctatcggca gctggtgtcg gaatgtctgt
       61 gagaacaggg aactgcggca gagaacctat gaagcggaga gaaccctgat cgacgggcag
      121 ggagctgccc ggatcgcaga gatcctgcag acggcgggaa tagaagaata gaaggataat
      181 atctgaggtt tatgaagaaa aacggcagct ggaagaaaaa aattttatat agtgtgacag
      241 cagctgtttt gttcgtgctg cttttggtac tgttttttcc gggggaccgg gaatatcaga
      301 ggattcctta tgatgtagtg tttctgggag acagtgtata tggtctgtgc cgtgatgaga
      361 catccatcgc ggcaaagctt caggagaaga caggtttaaa atgctacaac ggtgggctgg
      421 gaggaacggt tcttggcaga gcggatgcgg agagaaggct gggatatacc aaggattcca
      481 tttctgcggc cggactggta cgttcctttg tggtgaagga tttcggtgtc cagaggaccg
      541 tgcacatccg agaagcggcc acggactatt ttgcagatac tttgggagat ctgggacaga
      601 tcgattttga ccaggtgaag atcctgttta tcggttccgg actgaatgat tatcactccg
      661 gcactcccat cgaatccacg gcagatccgt atgatgaata tacctactgt ggagctatcc
      721 gcagtattgt gaaggagttg cgggaagcct atccggagct gcgcattatc ttcatcaccc
      781 cgccctatac ctggtatacg gatccggaac tgacctgtga ggaatatgat ctgggcggtg
      841 gtgtgctgga ggattatgtc aatgcggaaa tcgggctgtg tgaggctttg aatgtagaag
      901 tcatagacat ctatcatgac tattatccac atgatacctg ggatgatctg tatctctata
      961 ccgatgacgg cctgcatccc aatgaggccg gcagagagaa gatcgcacag acgatcgcgg
     1021 aaagactaaa taaggagtga cagacggatg aagaccccgt gggatttttt tcaatctagc
     1081 agaacaaaaa taaaaaatac atggaagatc gcctttctct cagcgtttgt actggggatt
     1141 ctgatccatc tgccggtgat gctttcggat attccgaatc atgacggcct gagcagtatg
     1201 tattttgatc agaatatgat cacttccgga cgatggtttc tgtctgtggc atgcggcttt
     1261 tcttcgtatt ttaccattcc gtggatcatt ggtctgatcg gtctgctctg gctggcgctg
     1321 gcggcggtgg cattgacgga actgctggaa cttgcagacc cgttggtaat catggcagtg
     1381 agcggattgc tggtttcttt tccggcactg gcatccactt ttgcctatgt gtttaccatg
     1441 gatggctata tgatggcact gttcctcgca attctggcag tgctttttac gaaaaaacag
     1501 aaaaaaggct ggctggccgg ggcggtatgt cttgcgttca gtattgggat ctatcaggct
     1561 tatctgccct gtgccattgt gctgtgcgta tatgtgatcc tgctctatat tatggatgag
     1621 aagggctgga agacgtaagc gttctatgta
//
LOCUS       DAWDHD010000279         1638 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            k141_7263_length_1638_cov_10.0000, whole genome shotgun sequence.
ACCESSION   DAWDHD010000279 DAWDHD010000000
VERSION     DAWDHD010000279.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7263_length_1638_cov_10.0000"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          341 a          475 c          348 g          474 t
ORIGIN      
        1 catacgctgt ttgtagcatt ctgcggtcag catacggtta acactgaagt gtgctcccac
       61 atctcttaac agttccacgt aattcaggtt caacagccag tccgcattgt tgaccatcag
      121 tgccttaccg tcactgaaat caataaaacg gctcatctgc ttcttgaagc agtctacgtt
      181 atgctggatc gtctccttgg tcatcatggt acgcatgtca cttctgccgg aaggatcacc
      241 aatcattgca gtaccaccgc cgatcagagc aatcggcttg ttgcctgcca tctgcattct
      301 cttcatcagg cacagtgcca tgaaatgtcc cacatgaagg ctgtctgcgg taggatcaaa
      361 gccgatgtag aaggtagcct ttccgttatt gatcagttcc ttgatctcct cttcatctgt
      421 cagctgtgcc agcagtcctc ttgcttttaa ttcgtcaaat actgtcattt tctcttctcc
      481 tttttgggtt ctttttactc tgtatacctt ttttacattt tgggggaatc ggctccaaag
      541 gcactaaaaa agcccgcccc tttttcaaaa ggacgagctt gatcactcgt gttaccacct
      601 aatgttcccg ccagactcac atctgacagc tcagtaagta tctatcaata ctttcggccg
      661 atatcggtgc ctgccgtcct gatctactcg ttcgggatcc tctgataaaa tctactgatc
      721 cttctctttc caccggctgc tccgggatgt attcctgctc tgcttctgtg cgcctctcac
      781 cgaccggcta ctctctgtca gaagtggagg aacagtactt cttcccttca tcgcattctt
      841 cttatcctat gtttgcgcta aacatgacct tattatagcc agtctgcgtt tttctgtcaa
      901 cacttaaaat tttgctgctg gcaggagccg cctacgggtc tattcttccg gccagggcag
      961 gtgggatact tccagcttct tgtcacgaag catcaattct tttaccgcct gatccgcggg
     1021 cttaccatca aagagcactt catttacctg ctcgataata ggcagctgaa catcatattt
     1081 tttcgacagt tccatggccg ctttagcgga atacacgccc tctaccacca tctgtacttc
     1141 cttcatggct tcctcgtagg tcttgccctg cccgatcagg ataccggcac gacggttacg
     1201 ggaatgcatg ctggcgcagg tcacgatcag atctccgata ccggtcagac cacagaaggt
     1261 ctcgaaatta ccacccatgg cagtaccaag tctggcgatc tcggtaatgc ctctggtgat
     1321 cagtgctgcc ttcgtattgt ctccgtaacc cagtccgtcg gcaataccgg cagccagcgc
     1381 tactacgttt ttcagggcac cgcccagttc aatgcccagt acatccgggc tggtatagac
     1441 acggaacacc tcgttcatga acagattctg cacatattcc gcacagcttt tactcttaga
     1501 acccgctacg atcgtcgtgg ggataccacg gctgacttcc tccgcgtgcg tcggtccgga
     1561 cagtaccgct atctgtgcct gggggatctc atcttcgatg atctgtgcct gggtcatcag
     1621 ggtatgctcc tcgattcc
//
LOCUS       DAWDHD010000280         1600 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_5240_length_1600_cov_12.523625, whole genome shotgun sequence.
ACCESSION   DAWDHD010000280 DAWDHD010000000
VERSION     DAWDHD010000280.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5240_length_1600_cov_12.523625"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          417 a          403 c          427 g          353 t
ORIGIN      
        1 gtccatcccg ggacccgtga tcctcagaca gaaccataac cccgcgaacc ccgccgcaca
       61 tgcagctacc accggtcccg agatctggga agacactgat ggaaaggttg atatcttcgt
      121 agcaggtgta ggtaccggcg gaaccgtgac cggcattggt acttatttaa aatccaagaa
      181 tcccaatgtg aagatcgtag cagtagagcc tgcagattct cccgtacttt ccggcggaca
      241 gcccggaccc cacaagctgc agggtatcgg tgccggattt gttccttccg ttctgaatac
      301 cggcatttat gatgagatct ttaccgttac taccgatcag gcatttacca ccggccgcct
      361 catcgctcat aaggagggca ttctggtagg aatcacctcc ggcgcagctt tgtacgcagc
      421 agcacagatc gcaaagcgcc ctgaaaatgc aggcaaaaca gtcgtagcat tgcttcccga
      481 ttccggtgac agatacctgt ccactcccat gtttgcagaa gcttaagacg aaatttgcag
      541 aactggacag acttgcattc ttgtgttaga atgtatggca tggtactagg aattcaagga
      601 atttgcgctt ttctcccctt aaaagcacga acctggaatg gaggaactta tggaaccgat
      661 cattcagatc aagaatgtaa caaaaacttt tataggtaag gataatcagg tagaggccct
      721 gaagggcatc agcctggata tcaacaaggg agatatctat ggcatcatcg gtatgagtgg
      781 tgccggaaag tctaccctgg tgcgttgcct gaactacctg gagaagccca ccaccggaac
      841 tgttgtggta gaggatcagg atctttcgac cttgacagag gcggaactcc gcaaggtgcg
      901 gacgggcatc gctatgatct ttcagcattt taacctgctc atgcagcgca gtgtcctcga
      961 caatgtgtgc ttccccatgg agatcgtggg tgtgaagaag caggcggcca gaaagcgcgc
     1021 tatggaactg ctcgagatcg taggactgaa ggaaaaggca gacgcttatc cttcccagct
     1081 ctccggtgga cagaagcagc gtgtggcaat cgcaagagct cttgccaata cgccgaagat
     1141 cctgctgtgt gatgaggcga ccagtgcgct ggatcccacg acgaccaagg agatcctgaa
     1201 attattacag gacatcaaca aacagtacgg catcaccatc gtgatcatca cccatgagat
     1261 ggctgtggta caggagatct gttcccatgt agccattatc gatgccggag aactggcaga
     1321 gcacggtact gtggaggagg tattctccag accccagtcc gcagccgcaa gaaagctggt
     1381 gttcatggtg gatcccaagg caaaggaaat gtccggcaaa cgctgcatcc gcattgtttt
     1441 cactgagaat tcttcttttg aacctgtgat cgccaatatg gtgctggagt gcaaggctcc
     1501 cgttaatatc ctgttagcgg ataccaagga catcggcggt atcgcccatg gacagatgat
     1561 cttacagctg ccggaggatg aaatggcggc gaacaagatg
//
LOCUS       DAWDHD010000281         1598 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_5249_length_1598_cov_6.771225, whole genome shotgun sequence.
ACCESSION   DAWDHD010000281 DAWDHD010000000
VERSION     DAWDHD010000281.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5249_length_1598_cov_6.771225"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          452 a          319 c          478 g          349 t
ORIGIN      
        1 catacatgag tggtaaaaac tgctttgtac gcacagggaa acctgtgcat cggctttatg
       61 aagtggacat ctttcgtgac gggaggaatc gcggtggcag cggccctgct tacttttgcg
      121 tattattact atcgcagcaa aaaagagttc ggcggcatca ccggagatac ggcggggtat
      181 tttgtgacgt tttgcgaagg atgcgtggtg gttgcggcag caatcgcaga acatatgatg
      241 ctgacagtgt aaaatgtatt gccggataga gtggtgcaga agcacatttg aaatgggaga
      301 gggaaatgat attaattatt ggcggctttg ctcagggaaa attacattat gtagagcagc
      361 attacgttca gcgtgaagac aggcaggaag tgccggttct ggatggaaca ctgaggctgg
      421 catcggatag taaaataaca tgggcaactg agagcgcggt tgaaacaatg cctggaaagc
      481 agacagagca gcctacaggg cggcagacgg ctggaccgca ggtgatcgtc aatcatctgc
      541 accgatatat ccgggagcag ttgcgacagg gaacagatcc ggaggcaatg atagcgaatt
      601 tctataagga atatccggac tgcattctga tctgtgacga gatcggcaac ggcatcgtcc
      661 ccatggaagc ggaggagaga acttacagag aatgcaccgg cagaattctg gaagggttgg
      721 cggcgcaggc agatgaggtg gtgagagtcg tatgcggaat cggccagaaa atcaaataga
      781 gatccgtctg accctgatcc ggcacggagc gactctttcc aataaagaag gacgttatct
      841 tggaaaaacg gatgaagcat tgagcccgga aggaatagga atgctgaaaa aagctgtgac
      901 gggaggaaac tatccgactg ctgaccttct gttttccggt cccatgaagc ggtgtctcga
      961 gacagcacag atcctgtatc cagggcagac tcccattttt gtcccggagt ggacggagat
     1021 ggatttcggt gcattcgaag gacacaatta tcaggaactt tccggggatc cggcatatca
     1081 gagatggatc gacagcggcg gaacactgcc gtttccggaa ggcgagagca gagaggaatt
     1141 cattcgcaga aacgttgccg gatatgaaaa aatgttatac tacatgaaaa tgatcttgga
     1201 gagaagtgct gcatctgagc agggtgatta caatacagga tcggagaaaa cggagttgga
     1261 aagaagcgat gaaattggag cacaggacgc cgggatacaa agcgtatccg cagtcgtcca
     1321 cggtggaacc atcatggcac tgctcagtca ttttctggga gagcaatatt ttaattatca
     1381 ggtaaaatgc ggacagggtt atagcggcag gctggtattc ccggcaggcg gaggaacccc
     1441 ggagtggaaa gaatttcgcc cactgtcgga atttacaaaa ggagaaacat attgatgaaa
     1501 tatcatattc tggcattcgg agcgggattt ttactggatc agctctttgg agatccctat
     1561 tttctgcccc atcccatccg gggaatcggc tggctgat
//
LOCUS       DAWDHD010000282         1524 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_5646_length_1524_cov_8.715453, whole genome shotgun sequence.
ACCESSION   DAWDHD010000282 DAWDHD010000000
VERSION     DAWDHD010000282.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5646_length_1524_cov_8.715453"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          424 a          320 c          433 g          347 t
ORIGIN      
        1 gctacagcag ctgtgatcgc catggctgct gcagctgttt acggaagtac gcaggcgatg
       61 agtcagacac aggagagcgg aagcggactg caactgtttc gaggaaagga gacaatctat
      121 tgctggtatt ccgatgaggc attgtccggt tatatcaatg cggcagcagt atccttcgga
      181 gaacagaaca gtggagtccg tgtgattccc gtacttactt ctgacagtga atatctggaa
      241 gctatcaatc aggagaccct gcattccgat cagataccgg atatctacct gttgagcagt
      301 gactctctgg aaaaggctta tctggcagga ctggcatcga aagtgcctga cactgtggga
      361 atctgcaata cagaccattt ttcccaggcg gctctgtctg cggtaaccta tgatcagaag
      421 ctgatcggtt acccggttta ttttgatacc agtgcgttgg tatataatga agattatctt
      481 cgaacctggg caacccagca ggcggagaag gaactggcgg gaagcagtga gaatgatgaa
      541 cccatagggg aaggagaaga gatcatcgag gaagattctc ttccggaaga ccagaccacg
      601 gaacaggtga cagcggatga ggcagcggta aatgcgctgg cagagcagta tttcgcggaa
      661 gcacttccct ccacagtgga tgatctgctg aatattgcag atacctttga tgcaccggaa
      721 ggtgtggaag gtgtcatgaa atgggacgtg aacaatattt tctacaacta ctggatcgta
      781 ggcaactaca tgatcgtagg cggtgatccc ggggatgaca gaaatgatat tgatatcaat
      841 aatccggaga ccattcagtg tctggaagtg tacaaggcac tgaaccagtt cttctttatt
      901 gaatccgata cggtgactta tgattctgtg atccaggact ttatcgatgg caagaccatg
      961 ttcaccatcg gaaccacgga tgtggtcaaa cggctggaag aggcaaaagc ggacggcagc
     1021 cttgcattta attacggaat tgcccgtatg ccggatgtca gcgccgaact gcagagcaga
     1081 tctttgtctg ttacgggagc ggcagtcatc aacagctatt cggaacacaa ggaactggcg
     1141 gactgcttcg cagcgtatct ggcagaagga tatgcggacg gcctgtatga acgctccggc
     1201 aaaatgccgg caagtcttca ggcggcagag aatgcagaca acggtgcgct tatgatcttt
     1261 gccgatgagt atgcggacag tgtgtcattg ccgaagatgc tagagacggg aaatttctgg
     1321 atgcagctgg aagtactgtt ttccaaggta tggaacggtg acgacgtgac tactctggta
     1381 cagcagctgg acgagaccat agccggacag gtaggtggta cagagaacta aaaccgttac
     1441 aataaaatat gaatgaacga accggtcaca ggatagacta ctactaaaaa gaaaaggtgg
     1501 tagaatccta tgatcggttt tttg
//
LOCUS       DAWDHD010000283         1508 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_2728
            NODE_5745_length_1508_cov_15.766690, whole genome shotgun sequence.
ACCESSION   DAWDHD010000283 DAWDHD010000000
VERSION     DAWDHD010000283.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996151
            Sequence Read Archive: SRR5898940
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5745_length_1508_cov_15.766690"
                     /isolate="gSpreadC_ID_2728"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="12.51 N 75 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          338 a          368 c          334 g          468 t
ORIGIN      
        1 ttattgatga aattccgtaa ggtctcttgg ggtggtgatg ctcattttct ctctgagatc
       61 tgccagcatc agggtttacc ggggttaata gatcatgggt ttatcgtaca ccggcatgat
      121 ctgcttggaa atcgcttttg tcaggggata cagtctggtt ccggaacctc ccgccagtat
      181 aattcctttc atttcgtcct ccgatccgcc cttttcgggc ttctttttac ttgtttttta
      241 acacaccgaa tgggaacgac gttcatcgtt cccatttaac aggtattctt tacttttctt
      301 cgtacatctc attgtagtac ttctggtaat caccgctggt cacattattc atccagtctt
      361 catgctcgaa gtaccaggcg atggtcttgc ggatgccttc cttgaacatg gtctcgggat
      421 accagccgat ctctgccttg atcttgtccg gtgcaattgc atatcttctg tcatgtccct
      481 tacgatcctc cacgtaagtg atcagatcgt tgttaatatg tgccttgcgg ggatcggaat
      541 ccggaagcat ctcctgcaga gtctcaatga taatgtggat gatctcgata ttctgcttct
      601 cattatgtcc gcccacattg taggtttcga acagtcttcc ctgttcctgt accatatcga
      661 tagccttagc atggtcttcc acatacagcc agtcacggac gttcttaccg tcaccgtaga
      721 ccggaagctt tttgccatgc aatgcattat tgatgatcag agggatcagc ttctcaggga
      781 actggaaagg accgtaattg ttggagcagt tggtaatgtt ggcagggaaa tgataggtat
      841 ccatatatgc cttcaccaac atgtccgagc ttgccttact tgcggagtaa gggctgtggg
      901 gatcatacgg agtggtctca tagaagaatg cattgtcatc atcgggaaga gatccgtaga
      961 cctcatctgt agatacatgc aggaacttct tgccttcttt gaagctgcca tcgggaagct
     1021 cccaggctgc cttggcacag ttcagcatca ctgcagtacc caggacattg gtctgtacga
     1081 agatctccgg gttgcggata cttctgtcta catggctttc tgccgcaaaa tgtaccacac
     1141 ggtcgatatc attctctgcg aaaattttgc tgatggcatc cttgtcacag atattagcct
     1201 tgacaaaggt gtagttctca cgcttctcca cgtccttgag attctcaaga ttgcctgcat
     1261 aggtcagtgc atccacgttg atgatacgga tttcattatc gtatttttta aacatgtaat
     1321 gaatatagtt ggaaccgata aaaccggccc ctcctgttac cagataagtt ctcatttttt
     1381 ctccgttctg cgcacagcgc acattttttg ttatagatag ggatatttta ccataaaaca
     1441 agcaggatat caacacggct cctccgcact tcgtgctctc ggatccgtga cacccattcg
     1501 cattccgg
//
